title: Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells.
abstract: We studied the effects of alfalfa saponin extract (ASE) on low density lipoprotein receptor (Ldlr), liver X receptor α (LXRα), and farnesoid X receptor (FXR) in normal and hyperlipidemic Buffalo rat liver (BRL) cells. Normal and hyperlipidemic BRL cells were divided into eight groups: normal, or normal cells treated with 50, 100, and 150 mg/L ASE, hyperlipidemic, or hyperlipidemic cells treated with 50, 100, and 150 mg/L ASE. After treatment for 24 h, Ldlr, LXRα, and FXR mRNA expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Data showed that mRNA expression of Ldlr in normal BRL cells was significantly up-regulated by ASE treatment and mRNA expressions of LXRα and FXR were significantly down-regulated both in normal and hyperlipidemic BRL cells after ASE treatment. Thus, ASE might ameliorate hepatic steatosis by regulating genes involved in cholesterol metabolism, including up-regulation of Ldlr as well as down-regulation of LXRα and FXR.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Xin-ping
authors.initials: XP
authors.lastname: Liang
authors.forename: Dong-qiang
authors.initials: DQ
authors.lastname: Zhang
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Cheng-zhang
authors.initials: CZ
authors.lastname: Wang
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Shi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006949:Hyperlipidemias / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D000071518:Liver X Receptors; D000455:Medicago sativa / Q000737:chemistry*; D057093:Orphan Nuclear Receptors / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*; D012503:Saponins / Q000008:administration & dosage*; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D010936:Plant Extracts; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011973:Receptors, LDL; D012503:Saponins; D000099150:Receptor, Farnesoid X-Activated; C000605215:Nr1h3 protein, rat
keywords: Alfalfa saponin extract; Cholesterol metabolism; Hyperlipidemic BRL cells; mRNA expression