title: Impact of fexofenadine, osthole and histamine on peripheral blood mononuclear cell proliferation and cytokine secretion.
abstract: This paper compares results of peripheral blood mononuclear cell (PBMC) incubation with fexofenadine (FXF) and osthole. FXF is a third-generation antihistamine drug and osthole is assumed a natural antihistamine alternative. To our best knowledge, this is the first comparative study on FXF, osthole and histamine cytokine secretion and cytotoxicity in PBMC in vitro cultures using cell proliferation ELISA BrdU. The cultures were treated 12, 42, 48 and 72h with FXF and osthole at 150, 300 and 450ng/ml concentrations and histamine at 50, 100 and 200ng/ml. Our study results confirm that FXF, osthole and histamine exert no cytotoxic effect on PBMCs and that IL-6, IL-10 and TNF-α cytokine secretion following osthole cell stimulation was similar to that by FXF stimulation.This confirms our hypothesis that osthole is a natural histamine antagonist, and can therefore be beneficially applied in antihistamine treatment.
authors.affiliation: Department of Biochemistry, Faculty of Biology & Biotechnology, University of Warmia and Mazury, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland. Electronic address: natalia.smulska@uwm.edu.pl.
authors.forename: Natalia
authors.initials: N
authors.lastname: Karolina Kordulewska
authors.affiliation: Department of Biochemistry, Faculty of Biology & Biotechnology, University of Warmia and Mazury, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland. Electronic address: elzbieta.kostyra@uwm.edu.pl.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kostyra
authors.affiliation: Department of Biochemistry, Faculty of Biology & Biotechnology, University of Warmia and Mazury, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland. Electronic address: michal.matysiewicz@uwm.edu.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Matysiewicz
authors.affiliation: Department of Biochemistry, Faculty of Biology & Biotechnology, University of Warmia and Mazury, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland. Electronic address: anna.cieslinska@uwm.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Cieślińska
authors.affiliation: Department of Biochemistry, Faculty of Biology & Biotechnology, University of Warmia and Mazury, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland. Electronic address: bj58@wp.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Jarmołowska
mesh_terms: D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003374:Coumarins / Q000494:pharmacology*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D003374:Coumarins; D016207:Cytokines; D006633:Histamine Antagonists; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D016593:Terfenadine; D006632:Histamine; C093230:fexofenadine; C046627:osthol
keywords: Allergic disease; Antihistamine drugs; Cultures in vitro; Cytokines secretion; Peripheral blood mononucleae cell (PBMC); Proliferation