title: Selective inhibition of the hypoxia-inducible factor prolyl hydroxylase PHD3 by Zn(II).
abstract: We report herein that Zn(II) selectively inhibits the hypoxia-inducible factor prolyl hydroxylase PHD3 over PHD2, and does not compete with Fe(II). Independent of the oligomer formation induced by Zn(II), inhibition of the activity of PHD3 by Zn(II) involves Cys42 and Cys52 residues distantly located from the active site.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. eunyang@kist.re.kr hschung@kist.re.kr.
authors.forename: Yu-Ran
authors.initials: YR
authors.lastname: Na
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Woo
authors.forename: Hyunah
authors.initials: H
authors.lastname: Choo
authors.forename: Hak Suk
authors.initials: HS
authors.lastname: Chung
authors.forename: Eun Gyeong
authors.initials: EG
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D005454:Fluorescence Polarization; D006801:Humans; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D064800:Prolyl-Hydroxylase Inhibitors / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism
chemical_list: D064800:Prolyl-Hydroxylase Inhibitors; C490190:EGLN1 protein, human; C488894:EGLN3 protein, human; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases; D015032:Zinc