title: Impact of Cell-free Supernatant of Lactic Acid Bacteria on Putrescine and Other Polyamine Formation by Foodborne Pathogens in Ornithine Decarboxylase Broth.
abstract: Conversion of ornithine to putrescine by Salmonella Paratyphi A, Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli was investigated in ornithine decarboxylase broth (ODB) using cell-free supernatants (CFSs) obtained from Leuconostoc mesenterodies subsp. cremoris, Pediococcus acidilactici, Lactococcus lactis subsp. lactis, Streptococcus thermophilus. Two groups of cell-free supernatants (25 or 50%) and control (only ODB) were prepared to investigate putrescine (PUT) and other polyamine formation by foodborne pathogens (FBPs). Significant differences (p < 0.05) were observed among the species for each amine. All of the CFSs reduced the formation of PUT by ≥65%. The production of cadaverine (CAD) was scarcely affected by the presence of CFSs, with the exception of the samples inoculated with L. monocytogenes. The variation in polyamine was found with respect to the control samples. Spermidine (SPD) was produced in lower amount in many samples, especially in Gram-negative FBPs, whereas spermine (SPN) increased drastically in the major part of the samples concerning the control. Histamine (HIS) was characterized by a marked concentration decrease in all of the samples, and tyramine (TYR) was accumulated in very low concentrations in the controls. Therefore, the ability of bacteria to produce certain biogenic amines such as HIS, TYR, PUT, and CAD has been studied to assess their risk and prevent their formation in food products. The results obtained from this study concluded that the lactic acid bacteria (LAB) strains with non-decarboxylase activity are capable of avoiding or limiting biogenic amine formation by FBP.
authors.affiliation: †Department of Seafood Processing Technology, Faculty of Fisheries, Cukurova University, Adana, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Ozogul
authors.affiliation: ‡Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Università degli Studi di Bologna, Sede di Cesena, Piazza Goidanich 60, 47521 Cesena (FC), Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Tabanelli
authors.affiliation: §Vocational School of Feke, Cukurova University, 01660 Feke, Adana, Turkey.
authors.forename: Nurten
authors.initials: N
authors.lastname: Toy
authors.affiliation: ‡Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Università degli Studi di Bologna, Sede di Cesena, Piazza Goidanich 60, 47521 Cesena (FC), Italy.|#Dipartimento di Scienze e Tecnologie Agro-alimentari, Università degli Studi di Bologna, Sede di Cesena, Piazza Goidanich 60, 47521 Cesena (FC), Italy.
authors.forename: Fausto
authors.initials: F
authors.lastname: Gardini
mesh_terms: D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005517:Foodborne Diseases / Q000382:microbiology; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D007777:Lactobacillaceae / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000201:enzymology / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011073:Polyamines; D019344:Lactic Acid; D009955:Ornithine Decarboxylase; D011700:Putrescine
keywords: cell-free supernatant; foodborne pathogens; lactic acid bacteria; ornithine decarboxylase broth; polyamine; putrescine