title: [Lice in Mali: frequency of infestation, genotyping, infection rate and case management].
abstract: OBJECTIVE
  Because lice-transmitted infections are a real public health problem, epidemiological studies in different ecoclimatic zones of Africa are useful. This article aims to describe the frequency of lice infestation, their genotypes, and their infection by pathogens in the regions of Koulikoro and Mopti.
  
  
  METHODS
  A cross-sectional survey allowed us to collect lice from rural populations. Techniques of molecular biology (real-time PCR, standard PCR, and genotyping) were used for analysis of lice samples.
  
  
  RESULTS
  Infestation rates were 57% (12/21) among subjects in Diankabou, in the Sahelian zone; 91% (39/43) in Doneguebougou, and 86% (59/69) in Zorocoro, in a savanna zone. The overall lice infestation rate in the samples in the three localities was 83% (110/133). Real-time PCR showed 3% (4/92) of Acinetobacter baumanii but no B. quintana in Diankabou. Phylogenetic analysis of the mitochondrial gene (Cytb) showed that head lice in Mali belong to genotype C.
  
  
  CONCLUSIONS
  The high frequency of lice infestation in the study population indicates that it would be useful to conduct national epidemiological surveys to estimate the magnitude of this public health problem.
authors.affiliation: 60, rue Sainte-Baume, 13010 Marseille, France, Malaria Research and Training Center, département d'épidémiologie des affections parasitaires, faculté de médecine et d'odonto-stomatologie, faculté de pharmacie (MRTC/DEAP/FMOS-FAPH). Université des sciences, des techniques et des technologies de Bamako (USTTB), Bamako, Mali, Unité de recherche sur les maladies infectieuses et tropicales émergentes (Urmite), université Aix Marseille, faculté de médecine, 27 bd Jean-Moulin, 13005, France.
authors.forename: A K
authors.initials: AK
authors.lastname: Sangaré
authors.affiliation: Malaria Research and Training Center, département d'épidémiologie des affections parasitaires, faculté de médecine et d'odonto-stomatologie, faculté de pharmacie (MRTC/DEAP/FMOS-FAPH). Université des sciences, des techniques et des technologies de Bamako (USTTB), Bamako, Mali.
authors.forename: S N
authors.initials: SN
authors.lastname: Doumbo
authors.affiliation: Malaria Research and Training Center, département d'épidémiologie des affections parasitaires, faculté de médecine et d'odonto-stomatologie, faculté de pharmacie (MRTC/DEAP/FMOS-FAPH). Université des sciences, des techniques et des technologies de Bamako (USTTB), Bamako, Mali.
authors.forename: A K
authors.initials: AK
authors.lastname: Koné
authors.affiliation: Malaria Research and Training Center, département d'épidémiologie des affections parasitaires, faculté de médecine et d'odonto-stomatologie, faculté de pharmacie (MRTC/DEAP/FMOS-FAPH). Université des sciences, des techniques et des technologies de Bamako (USTTB), Bamako, Mali.
authors.forename: M A
authors.initials: MA
authors.lastname: Thera
authors.affiliation: Malaria Research and Training Center, département d'épidémiologie des affections parasitaires, faculté de médecine et d'odonto-stomatologie, faculté de pharmacie (MRTC/DEAP/FMOS-FAPH). Université des sciences, des techniques et des technologies de Bamako (USTTB), Bamako, Mali.
authors.forename: A
authors.initials: A
authors.lastname: Dabo
authors.affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (Urmite), université Aix Marseille, faculté de médecine, 27 bd Jean-Moulin, 13005, France.
authors.forename: P
authors.initials: P
authors.lastname: Brouqui
authors.affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (Urmite), université Aix Marseille, faculté de médecine, 27 bd Jean-Moulin, 13005, France.
authors.forename: D
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Malaria Research and Training Center, département d'épidémiologie des affections parasitaires, faculté de médecine et d'odonto-stomatologie, faculté de pharmacie (MRTC/DEAP/FMOS-FAPH). Université des sciences, des techniques et des technologies de Bamako (USTTB), Bamako, Mali.
authors.forename: O K
authors.initials: OK
authors.lastname: Doumbo
mesh_terms: D000818:Animals; D019090:Case Management; D003430:Cross-Sectional Studies; D005838:Genotype; D006801:Humans; D010373:Lice Infestations / Q000453:epidemiology* / Q000628:therapy*; D008302:Mali / Q000453:epidemiology; D008003:Phthiraptera / Q000235:genetics*
keywords: Mali; Pediculus humanus; frequency; genotypes; therapy