title: Advanced nitrogen removal via nitrite using stored polymers in a modified sequencing batch reactor treating landfill leachate.
abstract: A modified sequencing batch reactor (SBR) operated at the anaerobic-aerobic-anoxic mode was developed in this study to fully utilize the organics in landfill leachate (ammonia concentration of 1000 ± 50 mg N/L and COD/total nitrogen (TN) ratio of 1-4). The unique feature of modified SBR process was the addition of an anaerobic stage after feeding stage, so that microorganisms could store the organics during anaerobic stage and supply the carbon source for endogenous denitritation after aeration stage. The 70-day operational tests showed the effluent TN was below 10 mg N/L at C/N ratio of 4. The intracellular stored polymers were analyzed and the microorganisms were capable of storing the carbon source as polyhydroxybutyrate (PHB) and glycogen in anaerobic stage, which were the electron donors for endogenous denitritation. Fluorescence in situ hybridization (FISH) analysis showed that glycogen accumulating organisms (GAOs) account for 39.8% of microorganisms in SBR, and carried out advanced nitrogen removal.
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. Electronic address: miaolei2819@emails.bjut.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Miao
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT, USA.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Tianhao
authors.initials: T
authors.lastname: Cao
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Tonglai
authors.initials: T
authors.lastname: Xue
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. Electronic address: pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000693:Anaerobiosis; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism; D004867:Equipment Design; D006003:Glycogen / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D009573:Nitrites / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification* / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009573:Nitrites; D011091:Polyesters; D014874:Water Pollutants, Chemical; D002244:Carbon; D006003:Glycogen; D009584:Nitrogen
keywords: FISH; GAOs; Landfill leachate; Nitrogen removal; SBR