title: Effect of mir-16 on proliferation and apoptosis in human A549 lung adenocarcinoma cells.
abstract: To investigate the expression of mir-16 in lung adenocarcinoma cancer line and to observe the effect of mir-16 on the biological behaviors of human lung adenocarcinoma cancer A549 cell. Methods the expression of mir-16 in A549 cells was examined by quantitative real-time (qRT)-PCR. mir-16 minics was chemically synthesized and transfected into A549 cells by Lipofectamine 2000. The cell cycle and apoptosis changes were assayed by flow cytometry, the cell proliferation was measured by MTS assay. The wild-type and mutant wip1 3'-UTR luciferase reporter rectors were constructed. The relative activity of renila luciferase was detected to confirm the binding site of mir-16 on wip1 mRNA. Results, the expression of mir-16 is reduced in A549 cell compared with the normal bronchial epithelial cell. Transfection of mir-16 minics significantly suppressed the luciferase reporter containing wild type not mutant wip1 3'-UTR. Furthermore enforced expression of mir-16 lead to reduced A549 cell proliferation and promote apoptosis. Conclusion Therapeutic strategies to resume miRNA-16 expression may be benefit to patients with NSCLC in the feature.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-Sen University Guangzhou 510080, Guangdong, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Ji-nan University Guangzhou 510630, Guangdong, P. R. China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-Sen University Guangzhou 510080, Guangdong, P. R. China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Lu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-Sen University Guangzhou 510080, Guangdong, P. R. China.
authors.forename: Yi-Yan
authors.initials: YY
authors.lastname: Lei
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-Sen University Guangzhou 510080, Guangdong, P. R. China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zou
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-Sen University Guangzhou 510080, Guangdong, P. R. China.
authors.forename: Hong-He
authors.initials: HH
authors.lastname: Luo
keywords: Mir-16; apoptosis; lung adenocarcinoma