title: 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity.
abstract: In the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass(®)) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass(®)/SeNp and 45S5Bioglass(®)/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.
authors.affiliation: Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia. Electronic address: magdalena.stevanovic@itn.sanu.ac.rs.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Stevanović
authors.affiliation: Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia.
authors.forename: Nenad
authors.initials: N
authors.lastname: Filipović
authors.affiliation: Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Djurdjević
authors.affiliation: Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia.
authors.forename: Miodrag
authors.initials: M
authors.lastname: Lukić
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Milenković
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
authors.forename: Aldo
authors.initials: A
authors.lastname: Boccaccini
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002516:Ceramics; D019344:Lactic Acid / Q000737:chemistry*; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012643:Selenium / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; C022107:Bioglass; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012643:Selenium
keywords: Antibacterial activity; Bioglass; PLGA; Scaffold; Selenium nanoparticles