title: Evaluation of the influence of three different temperatures on microleakage of two self-etch and one total-etch adhesives.
abstract: To evaluate the bonding temperature effect on dentin-restoration microleakage. The null hypothesis of the study is that the score of microleakage is identical among different adhesive bondings at different temperatures.
  
  
  
  Ninety caries free maxillary premolars were selected. Class V cavities were prepared on the cemento enamel junction (CEJ) of the buccal sides with enamel margins on occlusal sides and cementum margins on gingival sides. The specimens were divided into 3 groups: G1, single bond adhesive + Z250 composite; G2, P90 adhesive + Filtek Silorane composite; and G3, Clearfil SE bond + Clearfil APX. All groups were divided into three subgroups based on the adhesive temperature: A-4°C; B-25°C; and C-40°C. After coating the specimens with nail polish 1 mm beyond the margin of the restorations, they were stored in 0.5% basic Fuchsin dye solution for 24 hours. The teeth then were buccolingually sectioned and observed under a stereomicroscope.
  
  
  
  There was no significant difference between microleakage of occlusal and gingival margins in each group. Clearfil SE bond and Adper single bond displayed lower microleakage than P90 adhesive at 4°C and 25°C. The most and least microleakage score for Adper single bond was at 40°C and 25°C respectively. Clearfil SE bond showed less microleakage at 25°C than 4°C and 40°C.
  
  
  
  Clearfil SE bond and Adper single bond displayed less microleakage at 25°C while there was no significant difference among for P90 adhesive microleakage at three temperatures.
authors.affiliation: Department of Operative Dentistry, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Akbarian
authors.affiliation: Professor, Department of Operative Dentistry, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran, Phone: +989131415237, e-mail: sharafif@sums.ac.in.
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Sharafeddin
authors.affiliation: Department of Prosthodontics, College of Dental Medicine Nova South Eastern University, Fort Lauderdale, Florida, USA.
authors.forename: Golsa
authors.initials: G
authors.lastname: Akbarian
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003737:Dental Cavity Preparation / Q000379:methods; D003739:Dental Cementum / Q000648:ultrastructure; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification*; D003764:Dental Materials / Q000737:chemistry; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012394:Rosaniline Dyes; D062047:Silorane Resins / Q000737:chemistry; D013696:Temperature; D019237:Tooth Cervix / Q000648:ultrastructure
chemical_list: C112828:Clearfil AP-X; C438313:Clearfil SE Bond; D004396:Coloring Agents; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D008689:Methacrylates; D019279:Resin Cements; D012394:Rosaniline Dyes; D062047:Silorane Resins; C544925:filtek P90; C555513:silorane composite resin; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C025485:basic fuchsin
keywords: Adhesive temperature; Microleakage; Silorane composite.