title: Alpinia katsumadai Extracts Inhibit Adhesion and Invasion of Campylobacter jejuni in Animal and Human Foetal Small Intestine Cell Lines.
abstract: Alpinia katsumadai is used in traditional Chinese medicine for abdominal distention, pain, and diarrhoea. Campylobacter jejuni is the most common cause of bacterial food-borne diarrhoeal illnesses worldwide. Adhesion to gut epithelium is a prerequisite in its pathogenesis. The antimicrobial, cytotoxic, and anti-adhesive activities of a chemically characterised extract (SEE) and its residual material of hydrodistillation (hdSEE-R) from A. katsumadai seeds were evaluated against C. jejuni. Minimal inhibitory concentrations for SEE and hdSEE-R were 0.5 mg/mL and 0.25 mg/mL, respectively, and there was no cytotoxic influence in the anti-adhesion tests, as these were performed at much lower concentrations of these tested plant extracts. Adhesion of C. jejuni to pig (PSI) and human foetal (H4) small-intestine cell lines was significantly decreased at lower concentrations (0.2 to 50 µg/mL). In the same concentration range, the invasiveness of C. jejuni in PSI cells was reduced by 45% to 65% when they were treated with SEE or hdSEE-R. The hdSEE-R represents a bioactive waste with a high phenolic content and an anti-adhesive activity against C. jejuni and thus has the potential for use in pharmaceutical and food products.
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, 1000, Slovenia.|Medical Faculty, University of Maribor, Taborska ulica 8, Maribor 2000, Slovenia.
authors.forename: Maja Šikić
authors.initials: MŠ
authors.lastname: Pogačar
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, 1000, Slovenia.
authors.forename: Anja
authors.initials: A
authors.lastname: Klančnik
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitätsplatz 4,  A-8010, Graz, Austria.
authors.forename: Franz
authors.initials: F
authors.lastname: Bucar
authors.affiliation: Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311, Hoče, Slovenia.
authors.forename: Tomaž
authors.initials: T
authors.lastname: Langerholc
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, 1000, Slovenia.
authors.forename: Sonja Smole
authors.initials: SS
authors.lastname: Možina
mesh_terms: D032429:Alpinia / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016123:Campylobacter jejuni / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D007421:Intestine, Small / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: Alpinia katsumadai; Campylobacter jejuni; anti-adhesion; antimicrobial; cell cultures; distillation residue