title: Common prefrontal cortical gene expression profiles between adolescent SHR/NCrl and WKY/NCrl rats which showed inattention behavior.
abstract: Factor analyses of attention-deficit/hyperactivity (ADHD) symptoms divide the behavioral symptoms of ADHD into two separate domains, one reflecting inattention and the other, a combination of hyperactivity and impulsivity. Identifying domain-specific genetic risk variants may aid in the discovery of specific biological risk factors for ADHD. In contrast with data available on genes involved in hyperactivity and impulsivity, there is limited information on the genetic influences of inattention. Transcriptional profiling analysis in animal models of disorders may provide an important tool to identify genetic involvement in behavioral phenotypes. To explore some of the potential genetic underpinnings of ADHD inattention, we examined common differentially expressed genes (DEGs) in the prefrontal cortex of SHR/NCrl, the most validated animal model of ADHD and WKY/NCrl, animal model of ADHD-inattentive type. In contrast with Wistar rats, strain representing the "normal" heterogeneous population, SHR/NCrl and WKY/NCrl showed inattention behavior in the Y-maze task. The common DEGs in the PFC of SHR/NCrl and WKY/NCrl vs. Wistar rats are those involved in transcription (e.g. Creg1, Thrsp, Zeb2), synaptic transmission (e.g. Atp2b2, Syt12, Chrna5), neurological system process (e.g. Atg7, Cacnb4, Grin3a), and immune response (e.g. Atg7, Ip6k2, Mx2). qRT-PCR analyses validated expression patterns of genes representing the major functional gene families among the DEGs (Grin3a, Thrsp, Vof-16 and Zeb2). Although further studies are warranted, the present findings indicate novel genes associated with known functional pathways of relevance to ADHD which are assumed to play important roles in the etiology of ADHD-inattentive subtype.
authors.affiliation: Uimyung Research Institute for Neuroscience, Sahmyook University, 26-21 Kongreung-2-dong, Hwarangro-815, Nowon-gu, Seoul 139-742, Republic of Korea; Department of Pharmaceutical and Administrative Sciences, Loma Linda University, CA 92350, USA. Electronic address: idelapena@llu.edu.
authors.forename: Ike
authors.initials: I
authors.lastname: Dela Peña
authors.affiliation: Department of Neuroscience, School of Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Bang
authors.affiliation: Department of Neuroscience, School of Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Jinhee
authors.initials: J
authors.lastname: Lee
authors.affiliation: Uimyung Research Institute for Neuroscience, Sahmyook University, 26-21 Kongreung-2-dong, Hwarangro-815, Nowon-gu, Seoul 139-742, Republic of Korea.
authors.forename: June Bryan
authors.initials: JB
authors.lastname: de la Peña
authors.affiliation: Division of Child and Adolescent Psychiatry, Clinical Research Institute, Seoul National University Hospital, 28 Yungundong, Chongrogu, Seoul 110-744, Republic of Korea.
authors.forename: Bung-Nyun
authors.initials: BN
authors.lastname: Kim
authors.affiliation: Department of Psychiatry, Chung-Ang University Medical School, 102 Heukseok-ro, Dongjak-gu, Seoul 156-755, Republic of Korea.
authors.forename: Doug Hyun
authors.initials: DH
authors.lastname: Han
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Noh
authors.affiliation: Department of Neuroscience, School of Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Shin
authors.affiliation: Uimyung Research Institute for Neuroscience, Sahmyook University, 26-21 Kongreung-2-dong, Hwarangro-815, Nowon-gu, Seoul 139-742, Republic of Korea. Electronic address: cheongjh@syu.ac.kr.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Cheong
mesh_terms: D000818:Animals; D001288:Attention / Q000502:physiology*; D001289:Attention Deficit Disorder with Hyperactivity / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D018782:Maze Learning / Q000502:physiology*; D009483:Neuropsychological Tests; D017397:Prefrontal Cortex / Q000254:growth & development / Q000378:metabolism*; D040081:Protein Array Analysis; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013045:Species Specificity
keywords: ADHD; Gene expression; Inattention; Prefrontal cortex; SHR/NCrl; WKY/NCrl