title: A miniaturized blotting system for simultaneous detection of different autoantibodies.
abstract: Sera of tumor patients frequently contain autoantibodies to tumor associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.
authors.affiliation: Institut für Immunologie, Carl Gustav Carus Technische Universität Dresden, Germany, Fetscherstrasse 74, 01307, Dresden, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Canzler
authors.forename: Holger
authors.initials: H
authors.lastname: Bartsch
authors.forename: Kai
authors.initials: K
authors.lastname: Großmann
authors.forename: Werner
authors.initials: W
authors.lastname: Lehmann
authors.forename: Karsten
authors.initials: K
authors.lastname: Conrad
authors.forename: Biji T
authors.initials: BT
authors.lastname: Kurien
authors.forename: Yaser
authors.initials: Y
authors.lastname: Dorri
authors.forename: R Hal
authors.initials: RH
authors.lastname: Scofield
authors.forename: Michael P
authors.initials: MP
authors.lastname: Bachmann
mesh_terms: D001323:Autoantibodies / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D001419:Bacteria / Q000235:genetics; D015153:Blotting, Western / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000737:chemistry; D006801:Humans; D008904:Miniaturization / Q000379:methods*; D013997:Time Factors
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D006639:Histidine