title: B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells.
abstract: The B-cell ELISPOT assay is a sensitive tool that can be utilized to measure total immunoglobulin (Ig) and antigen-specific antibody-secreting cells. Typically, membrane-bound antigen enables binding of antibody secreted by B-cells. Bound antibody is then detected by using an anti-Ig antibody and a colorimetric substrate, resulting in colored spots on the membrane that can be easily enumerated. Here we have described a method to quantitate antigen-specific antibody-secreting cells from the spleen or bone marrow of a vaccinated mouse.
authors.affiliation: Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA.
authors.forename: Hemangi B
authors.initials: HB
authors.lastname: Shah
authors.forename: Kristi A
authors.initials: KA
authors.lastname: Koelsch
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002469:Cell Separation; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D051379:Mice; D014611:Vaccination
chemical_list: D000941:Antigens