title: The crux and crust of ebolavirus: Analysis of genome sequences and glycoprotein gene.
abstract: The recent 2013-15 epidemic of Ebola virus disease (EVD) has initiated extensive sequencing and analysis of ebolavirus genomes. All ebolavirus genomes available until December 2014 have been collated and analyzed in this study to obtain phylogenetic relationship and uncover the variations amongst them. The terminal 'leader' and 'trailer' nucleotide sequences of the genomes were omitted and analysis of the intermediate region accommodating the sole seven genes (hepta-CDS region) of the virus showed relative stability of the genome, including the ones isolated from the current epidemic. The genome information was scrutinized to detect the variation in the surface glycoprotein gene and annotate its three protein products, resulting from its atypical transcription. This study will make an easy understanding of the genomes for those who desire to exploit the genome sequences for different investigations in EVD.
authors.affiliation: National Centre for Cell Science, SP Pune University Campus, Pune, 411007, India. Electronic address: kkmahale@gmail.com.
authors.forename: Kiran Narasinha
authors.initials: KN
authors.lastname: Mahale
authors.affiliation: National Centre for Cell Science, SP Pune University Campus, Pune, 411007, India. Electronic address: patole@nccs.res.in.
authors.forename: Milind S
authors.initials: MS
authors.lastname: Patole
mesh_terms: D029043:Ebolavirus / Q000235:genetics*; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006031:Glycosylation; D010802:Phylogeny; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins
keywords: GP-gene; Genome phylogeny; Glycosylation; RNA polymerase slippage