title: Fibroblast-Negative CD34-Negative Cells from Human Adipose Tissue Contain Mesodermal Precursors for Endothelial and Mesenchymal Cells.
abstract: There is considerable evidence that stem/progenitor cells reside in the vasculature during the prenatal and postnatal stages. The stromal vascular fraction (SVF) of human adipose tissue is markedly rich in blood vessels, and it is a source of mesenchymal/stromal cells (MSCs). Therefore, we hypothesized that, in addition to MSCs, the SVF may contain other mesodermal precursors. However, the SVF has a high content of CD34(+) cells with high proliferative capacity, which can prevent the growth of the most quiescent cells. By using an antifibroblast (FIB) antibody coupled to microbeads, we show that ∼ 90%-95% of the nonhematopoietic CD34(+) cells were retained in the CD45(-)FIB(+) fraction. Reverse transcription-polymerase chain reaction analysis revealed that the CD45(-)FIB(-)CD34(-) cell fraction expressed higher mRNA levels of KDR and GATA2 than its complementary CD45(-)FIB(-)CD34(+) cell fraction, which contained the SVF endothelial cells. Surprisingly, when CD45(-)FIB(-)CD34(-) cells were cultured in endothelial growth medium, they gave rise to endothelial colonies and mesenchymal colonies. Moreover, when CD45(-)FIB(-)CD34(-) cells were cultured in embryonic stem cell expansion medium, they gave rise to cells exhibiting the full range of phenotypes observed in the freshly isolated SVF, including CD34(+) and CD31(+) cells. Together, these results suggest that the CD45(-)FIB(-)CD34(-) cells within the SVF of human adipose tissue function as mesodermal precursors of mesenchymal and endothelial cells.
authors.affiliation: 1 Regenerative Medicine Laboratory, Fundación Hospital General Universitario , Valencia, Spain .
authors.forename: Amparo
authors.initials: A
authors.lastname: Navarro
authors.affiliation: 2 Department of Plastic and Reconstructive Surgery, Consorcio Hospital General Universitario , Valencia, Spain .
authors.forename: Severiano
authors.initials: S
authors.lastname: Marín
authors.affiliation: 3 Immunohematology Service , Centro de Transfusiones, Valencia, Spain .
authors.forename: Nicasia
authors.initials: N
authors.lastname: Riol
authors.affiliation: 3 Immunohematology Service , Centro de Transfusiones, Valencia, Spain .
authors.forename: Francisco
authors.initials: F
authors.lastname: Carbonell-Uberos
authors.affiliation: 1 Regenerative Medicine Laboratory, Fundación Hospital General Universitario , Valencia, Spain .
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Miñana
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply / Q000166:cytology* / Q000378:metabolism; D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D050989:GATA2 Transcription Factor / Q000235:genetics; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors