title: MiR-592 inhibited cell proliferation of human colorectal cancer cells by suppressing of CCND3 expression.
abstract: Accumulating evidence shows that microRNA (miRNA) is frequently associated with multiple kinds of human cancers, including colorectal cancer (CRC). Previous studies have shown that miR-592 play critical roles in cancer cell biological processes. However, the function of miR-592 in CRC remains largely unknown. In the present study, we investigated the miR-592's role in cell proliferation of colorectal cancer. MiR-592 expression was markedly down-regulated in CRC tissues and CRC cells. Overexpression of miR-592 reduced the proliferation and anchorage-independent growth of CRC cells. Furthermore, bioinformatics analysis further revealed CCND3, a putative tumor promoter, was found to be a potential target of miR-592 in CRC. The dual-luciferase reporter gene assay results showed that CCND3 was a direct target of miR-592. Ectopic expression of miR-592 led to down-regulation of CCND3 protein, which resulted in the down-regulation of phosphorylated retinoblastoma (p-Rb). In functional assays, CCND3-silenced in miR-592-in-transfected SW48 cells have positive effect to suppress cell proliferation, suggesting that direct CCND3 suppression is required for miR-592-induced cell proliferation of CRC. We conclude that miR-592 can regulate CCND3 and function as a tumor suppressor in CRC. Therefore, miR-592 represents a potential anti-onco-miR and serves as a useful therapeutic agent for miRNA-based CRC therapy.
authors.affiliation: Department of Cadre Health Protection, Liaocheng People's Hospital Liaocheng 252000, China.
authors.forename: Zhehui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Cadre Health Protection, Liaocheng People's Hospital Liaocheng 252000, China.
authors.forename: Ruiqin
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of Neurology, Liaocheng People's Hospital Liaocheng 252000, China.
authors.forename: Guanzeng
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Cadre Health Protection, Liaocheng People's Hospital Liaocheng 252000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Cadre Health Protection, Liaocheng People's Hospital Liaocheng 252000, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Cadre Health Protection, Liaocheng People's Hospital Liaocheng 252000, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
keywords: CCND3; cell proliferation; colorectal cancer; miR-592