title: Western blotting using microfluidics.
abstract: Together with polyacrylamide gel electrophoresis, the western blot has been an invaluable research technique in biological sciences. It continues to serve as an important diagnostic tool in medical laboratories. The procedure, however, involves multiple steps that are often time and resource intensive in addition to being of low throughput. Using advances in microfluidics, Hughes and Herr et al. initially developed a microfluidic western blot approach that significantly optimizes resources and assay times. More recent developments have enabled multiplexing to facilitate probing of multiple proteins.
authors.affiliation: Division of Cardiovascular Sciences, National Heart, Lung and Blood Institute, National Institutes of Health, 6701 Rockledge Drive, Room 10214, MSC 7936, Bethesda, MD, 20892-7956, USA, srinivap@nhlbi.nih.gov.
authors.forename: Pothur R
authors.initials: PR
authors.lastname: Srinivas
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification
chemical_list: D011506:Proteins