title: Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies.
abstract: Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human β2-glycoprotein I and prothrombin. The resulting phospholipid-protein conjugates show high binding affinity and specificity for the autoimmune antibodies against autoimmune complexes. Thus, the development of this work might become a milestone in further diagnostics and therapy of autoimmune diseases that involve the production of autoantibodies against the aforementioned phospholipids and proteins, such as antiphospholipid syndrome and systemic lupus erythematosus.
authors.affiliation: Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, Odense 5230, Denmark. arindam.ju05@gmail.com.|Dr. B C Roy College of Pharmacy and AHS, Durgapur, West Bengal 713212, India. arindam.ju05@gmail.com.
authors.forename: Arindam
authors.initials: A
authors.lastname: Maity
authors.affiliation: Divisions of Human Gene Therapy and Pediatric Rheumatology, Program in Immunology, Stanford University School of Medicine, 269 Campus Drive, Stanford, MC 5164, USA. macaubas@stanford.edu.
authors.forename: Claudia
authors.initials: C
authors.lastname: Macaubas
authors.affiliation: Divisions of Human Gene Therapy and Pediatric Rheumatology, Program in Immunology, Stanford University School of Medicine, 269 Campus Drive, Stanford, MC 5164, USA. mellins@stanford.edu.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Mellins
authors.affiliation: Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, Odense 5230, Denmark. ias@sdu.dk.
authors.forename: Kira
authors.initials: K
authors.lastname: Astakhova
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000936:Antigen-Antibody Complex / Q000737:chemistry; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D004983:Ethanolamines / Q000737:chemistry*; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D011485:Protein Binding; D011516:Prothrombin / Q000737:chemistry*; D012996:Solutions; D014230:Triazoles / Q000737:chemistry; D053482:beta 2-Glycoprotein I / Q000737:chemistry*
chemical_list: D000480:Alkynes; D000936:Antigen-Antibody Complex; D000941:Antigens; D001323:Autoantibodies; D001386:Azides; D004983:Ethanolamines; D018796:Immunoconjugates; D012996:Solutions; D014230:Triazoles; D053482:beta 2-Glycoprotein I; C005448:phosphorylethanolamine; D011516:Prothrombin
keywords: CuAAC click chemistry; antigens; antiphospholipid syndrome; phosphoethanolamine; prothrombin; β2-glycoprotein I