title: Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulinaplatensis.
abstract: The invitro scavenger activities of different reactive oxygen species (superoxide radical, hydroxyl radical, hydrogen peroxide, hypochlorous acid and peroxyl radical), the effects on lipid peroxidation and the iron-chelating ability of a Spirulinaplatensis protean extract and the biliprotein, phycocyanin, isolated from this microalga were studied. S. platensis protean extract inhibited the generation of hydroxyl radical (IC50=537μg/ml for the system with EDTA and 1500μg/ml without EDTA), the production of peroxyl radical (IC50=230μg/ml), and the lipid peroxidation process (IC50=2320μg/ml for the enzymatic system and 2180μg/ml for the non-enzymatic system). Besides, phycocyanin inhibited hydroxyl and peroxyl radicals and the lipid peroxidation process. The iron ions decreased the maximum fluorescence emission spectra of S. platensis protean extract and phycocyanin and it was an indicator of the metal-chelating activity. The antioxidant properties of S. platensis and phycocyanin may arise from both radical-scavenging and metal chelation. Our results suggest that S. platensis could be used as a dietary supplement to prevent some diseases where free radicals are involved.
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain. Electronic address: bescos@farm.ucm.es.
authors.forename: Paloma
authors.initials: P
authors.lastname: Bermejo
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Piñero
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Ángel M
authors.initials: ÁM
authors.lastname: Villar
keywords: Free radical scavenger; Iron-chelating activity; Lipid peroxidation; Phycocyanin; Spirulinaplatensis