title: High-density mapping of quantitative trait loci for grain-weight and spikelet number in rice.
abstract: BACKGROUND
  High grain yield is one of the most important traits requiring improvement in rice breeding programs. Consequently, the genetic basis of spikelets per panicle (SPP) and grain weight (TGW) have received much research focus because of their importance in rice yield.
  
  
  RESULTS
  In this study, IL28, which is a near isogenic line (NIL) developed by introgressing chromosomal segments of the cultivar 'Moroberekan' into the cultivar 'Ilpumbyeo', showed a significant increase in the number of spikelets per panicle (SPP) and 1,000-grain weight (TGW) compared to the recurrent parent, Ilpumbyeo. Quantitative trait locus (QTL) analysis in 243 F2 plants derived from a cross between IL28 and Ilpumbyeo indicated that both qSPP6 and qTGW6 are located in the interval RM3430-RM20580. Following substitution mapping with 50 F3:4:5 lines, qSPP6 was mapped to a 429-kb interval between RM20521 and InDel-1, while qTGW6 was mapped to a 37.85-kb interval between InDel-1 and SNP--3 based on the japonica genome sequence. This result indicates that qSPP6 and qTGW6 are different genes. Yield trials with substitution lines indicated that lines harboring the homozygous Moroberekan segment at both the qSPP6 and qTGW6 region showed significantly higher grain yield than Ilpumbyeo.
  
  
  CONCLUSIONS
  Because the Moroberekan alleles for SPP and TGW have been shown to be beneficial in the genetic background of Ilpumbyeo, both the qSPP6 and qTGW6 alleles might prove valuable in improving rice yields. Closely linked SSR markers are expected to facilitate the cloning of genes that underlie these QTLs, as well as with marker-assisted selection for variation in SPP and TGW in rice breeding programs.
authors.affiliation: Department of Agronomy, College of Agriculture & Life Sciences, Chungnam National University, Daejeon, 305-764, South Korea, acekdm@naver.com.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Mark Edward
authors.initials: ME
authors.lastname: Fabreag
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Yeo-Tae
authors.initials: YT
authors.lastname: Yun
authors.forename: Chong-Tae
authors.initials: CT
authors.lastname: Chung
authors.forename: Sang-Nag
authors.initials: SN
authors.lastname: Ahn