title: Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop.
abstract: We recently reported the induction of potent, cross-clade neutralizing antibodies (nAbs) against Human Immunodeficiency Virus type-1 (HIV-1) in rabbits using gp120 based on an M-group consensus sequence. To better characterize these antibodies, 93 hybridomas were generated, which represent the largest panel of monoclonal antibodies (mAbs) ever generated from a vaccinated rabbit. The single most frequently recognized epitope of the isolated mAbs was at the very C-terminal end of the protein (APTKAKRRVVEREKR), followed by the V3 loop. A total of seven anti-V3 loop mAbs were isolated, two of which (10A3 and 10A37) exhibited neutralizing activity. In contrast to 10A3 and most other anti-V3 loop nAbs, 10A37 was atypical with its epitope positioned more towards the C-terminal half of the loop. To our knowledge, 10A37 is the most potent and broadly neutralizing anti-V3 loop mAb induced by vaccination. Interestingly, all seven anti-V3 loop mAbs competed with PGT121, suggesting a possibility that early induction of potent anti-V3 loop antibodies could prevent induction of more broadly neutralizing PGT121-like antibodies that target the conserved base of the V3 loop stem.
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Yali
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Saikat
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Aditi
authors.initials: A
authors.lastname: Agrawal
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Heliang
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Marisa
authors.initials: M
authors.lastname: Banasik
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Kari
authors.initials: K
authors.lastname: Rohl
authors.affiliation: Department of Surgery, Duke University, Durham, NC, 27710, United States of America.
authors.forename: Celia
authors.initials: C
authors.lastname: LaBranche
authors.affiliation: Department of Surgery, Duke University, Durham, NC, 27710, United States of America.
authors.forename: David C
authors.initials: DC
authors.lastname: Montefiori
authors.affiliation: Department of Biomedical Sciences, Iowa State University, Ames, IA, 50011, United States of America; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, Iowa State University, Ames, IA, 50011, United States of America.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D017124:Conserved Sequence; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D015699:HIV Envelope Protein gp120 / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011817:Rabbits; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120