title: Development and characterization of a novel GHR antibody antagonist, GF185.
abstract: Here, we describe the development of a panel of monoclonal antibodies targeting the growth hormone receptor (GHR). Of these monoclonal antibodies (Mabs), GF185 was selected for further characterization due to its activities. Competitive receptor-binding assays and Western blotting analyses were used to demonstrate that GF185's epitopes are localized within subdomain 1 of the growth hormone receptor extracellular domain (GHR-ECD). Subsequently, we evaluated GF185's antagonistic activities in vivo and in vitro and showed that GF185 was able to neutralize growth hormone (GH) signalling and inhibit GH-induced Ba/F3-GHR proliferation. Our findings suggest that GF185 may serve as an attractive tool for GHR-related research and has a potential future application for the treatment of GH-dependent disease.
authors.affiliation: Department of Endocrinology, The First People's Hospital, Jining 272000, Shandong, PR China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, The First People's Hospital, Jining 272000, Shandong, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Central Laboratory, The First People's Hospital, Jining 272000, Shandong, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Traumatology Department, The First People's Hospital, Jining 272000, Shandong, PR China. Electronic address: tianbaofangtougao@163.com.
authors.forename: Baofang
authors.initials: B
authors.lastname: Tian
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D016466:CHO Cells; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000276:immunology*; D049109:Cell Proliferation; D003412:Cricetulus; D000939:Epitopes / Q000276:immunology*; D013006:Growth Hormone / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015398:Signal Transduction
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D002352:Carrier Proteins; D000939:Epitopes; D013006:Growth Hormone; C051815:somatotropin-binding protein
keywords: Anti-GHR antagonist; Growth hormone; Growth hormone receptor