title: SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients.
abstract: Diffuse large B-cell lymphoma (DLBCL), the most common lymphoma, is a heterogeneous disease where the outcome for patients with early relapse or refractory disease is very poor, even in the era of immunochemotherapy. In order to describe possible differences in global protein expression and network patterns, we performed a SILAC-based shotgun (LC-MS/MS) quantitative proteomic analysis in fresh-frozen tumor tissue from two groups of DLBCL patients with totally different clinical outcome: (i) early relapsed or refractory and (ii) long-term progression-free patients. We could identify over 3,500 proteins; more than 1,300 were quantified in all patients and 87 were significantly differentially expressed. By functional annotation analysis on the 66 proteins overexpressed in the progression-free patient group, we found an enrichment of proteins involved in the regulation and organization of the actin cytoskeleton. Also, five proteins from actin cytoskeleton regulation, applied in a supervised regression analysis, could discriminate the two patient groups. In conclusion, SILAC-based shotgun quantitative proteomic analysis appears to be a powerful tool to explore the proteome in DLBCL tumor tissue. Also, as progression-free patients had a higher expression of proteins involved in the actin cytoskeleton protein network, such a pattern indicates a functional role in the sustained response to immunochemotherapy.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden ; Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden.
authors.forename: Ulla
authors.initials: U
authors.lastname: Rüetschi
authors.affiliation: Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden ; Section of Hematology, Kungälv Hospital, 442 83 Kungälv, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Stenson
authors.affiliation: Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden ; Section of Hematology and Coagulation, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden.
authors.forename: Sverker
authors.initials: S
authors.lastname: Hasselblom
authors.affiliation: Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden ; Section of Hematology and Coagulation, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden.
authors.forename: Herman
authors.initials: H
authors.lastname: Nilsson-Ehle
authors.affiliation: Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden ; Department of Pathology and Cytology, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Hansson
authors.affiliation: Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden ; Department of Pathology and Cytology, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Fagman
authors.affiliation: Sahlgrenska Academy, University of Gothenburg, 413 45 Göteborg, Sweden ; Unit of Hematology, Department of Medicine, Södra Älvsborg Hospital, 504 55 Borås, Sweden.
authors.forename: Per-Ola
authors.initials: PO
authors.lastname: Andersson