title: Transdermal Delivery of Functional Collagen Via Polyvinylpyrrolidone Microneedles.
abstract: Collagen makes up a large proportion of the human body, particularly the skin. As the body ages, collagen content decreases, resulting in wrinkled skin and decreased wound healing capabilities. This paper presents a method of delivering type I collagen into porcine and human skin utilizing a polyvinylpyrrolidone microneedle delivery system. The microneedle patches were made with concentrations of 1, 2, 4, and 8% type I collagen (w/w). Microneedle structures and the distribution of collagen were characterized using scanning electron microscopy and confocal microscopy. Patches were then applied on the porcine and human skin, and their effectiveness was examined using fluorescence microscopy. The results illustrate that this microneedle delivery system is effective in delivering collagen I into the epidermis and dermis of porcine and human skin. Since the technique presented in this paper is quick, safe, effective and easy, it can be considered as a new collagen delivery method for cosmetic and therapeutic applications.
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA.|Cardiovascular Pharmacology Division, Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Sun
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA.|Cardiovascular Pharmacology Division, Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Inayathullah
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA.|Department of Dermatology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Martin A C
authors.initials: MA
authors.lastname: Manoukian
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Malkovskiy
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA.
authors.forename: Sathish
authors.initials: S
authors.lastname: Manickam
authors.affiliation: Department of Dermatology, Stanford University School of Medicine, Stanford, CA, 94305, USA.|Division of Dermatology, Palo Alto VA Medical Center, Palo Alto, CA, 94304, USA.
authors.forename: M Peter
authors.initials: MP
authors.lastname: Marinkovich
authors.affiliation: Department of Dermatology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Alfred T
authors.initials: AT
authors.lastname: Lane
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA.|Department of Developmental Sciences, Marquette University School of Dentistry, Milwaukee, WI, 53201, USA.
authors.forename: Lobat
authors.initials: L
authors.lastname: Tayebi
authors.affiliation: Division of Surgery and Interventional Science, University College London, London, UK.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Seifalian
authors.affiliation: Biomaterials and Advanced Drug Delivery Laboratory, Stanford University School of Medicine, 1050 Arastradero Road, Building A, Room A148, Palo Alto, CA, 94304, USA. jayraja@stanford.edu.|Cardiovascular Pharmacology Division, Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA. jayraja@stanford.edu.
authors.forename: Jayakumar
authors.initials: J
authors.lastname: Rajadas
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D024042:Collagen Type I / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008845:Microinjections; D009339:Needles; D011205:Povidone; D012867:Skin / Q000378:metabolism; D013552:Swine
chemical_list: D024042:Collagen Type I; D011205:Povidone
keywords: Biomaterials; Collagen; Microneedles; Transdermal delivery