title: Criteria and challenges of the human placental perfusion – Data from a large series of perfusions.
abstract: Perfusion of human placental cotyledon has been used extensively to study transplacental transfer of endogenous and exogenous compounds. However, many challenges in the use of the method exist, including availability of placentas and complexity of the method itself. In Kuopio, Finland we have carried out human placental perfusions since 2005 using the same method with data now from over one hundred perfusions. This has allowed us to study whether the way of delivery, placental weight, and/or the length of pregnancy affect the two major criteria of a successful perfusion: volume loss (leak) from fetal to maternal circulation, and transplacental transfer of the reference compound antipyrine. The only statistically significant result was the reduction of the fetomaternal ratio of antipyrine by the placental age over 40 weeks (p=0.0004). The success criteria were not affected by the weight of the placenta or the way of delivery. There was no effect by the antipyrine concentration on antipyrine transfer. In vitro incubation with different concentrations of study compounds and different tubing materials could offer an easy way to study potentially reduced recovery due to binding to perfusion system.
authors.affiliation: Faculty of Health Sciences, School of Pharmacy/Toxicology, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland. Electronic address: vesa.karttunen@uef.fi.
authors.forename: V
authors.initials: V
authors.lastname: Karttunen
authors.affiliation: Faculty of Health Sciences, School of Pharmacy/Toxicology, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Sahlman
authors.affiliation: Faculty of Health Sciences, School of Pharmacy/Toxicology, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
authors.forename: J K
authors.initials: JK
authors.lastname: Repo
authors.affiliation: School of Biological Sciences, Faculty of Science, Kadoorie Biological Sciences Building, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: C S J
authors.initials: CS
authors.lastname: Woo
authors.affiliation: Faculty of Health Sciences, School of Pharmacy/Toxicology, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Myöhänen
authors.affiliation: Center for Arctic Medicine, Thule Institute, University of Oulu, Finland; Nordlab Oulu, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Myllynen
authors.affiliation: Faculty of Health Sciences, School of Pharmacy/Toxicology, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
authors.forename: K H
authors.initials: KH
authors.lastname: Vähäkangas
mesh_terms: D000983:Antipyrine / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008431:Maternal-Fetal Exchange; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D000983:Antipyrine
keywords: Antipyrine; Leak; Placental age; Placental weight; Recovery