title: Sulfonated Polyethylenimine for Photosensitizer Conjugation and Targeting.
abstract: Polysulfonated macromolecules are known to bind selectins, adhesion membrane proteins which are broadly implicated in inflammation. Commercially available branched polyethylenimine (PEI) was reacted with chlorosulfonic acid to generate sulfonated PEI with varying degrees of sulfonation. Remaining unreacted amine groups were then used for straightforward conjugation with pyropheophoribide-a, a near-infrared photosensitizer. Photosensitizer-labeled sulfonated PEI conjugates inhibited blood coagulation and were demonstrated to specifically bind to cells genetically programmed to overexpress L-selectin (CD62L) or P-selectin (CD62P). In vitro, following targeting, selectin-expressing cells could be destroyed via photodynamic therapy.
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Upendra
authors.initials: U
authors.lastname: Chitgupi
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Yumiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Chi Y
authors.initials: CY
authors.lastname: Lo
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shao
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Wentao
authors.initials: W
authors.lastname: Song
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Jumin
authors.initials: J
authors.lastname: Geng
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Sriram
authors.initials: S
authors.lastname: Neelamegham
authors.affiliation: †Department of Biomedical Engineering and ‡Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: Lovell
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D015316:Genetic Therapy; D019041:L-Selectin / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D019007:P-Selectin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D019007:P-Selectin; D017319:Photosensitizing Agents; D011108:Polymers; D013451:Sulfonic Acids; D019041:L-Selectin; D011094:Polyethyleneimine