title: An effective plasma membrane proteomics approach for small tissue samples.
abstract: Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale tissue.
authors.affiliation: KU Leuven, Department of Biology, Laboratory of Neuroplasticity and Neuroproteomics, Leuven, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: Smolders
authors.affiliation: KU Leuven, Department of Biology, Laboratory of Neuroplasticity and Neuroproteomics, Leuven, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lombaert
authors.affiliation: 1] Unit Environmental Risk &Health, VITO, Mol, Belgium [2] Center for Proteomics, UAntwerp, Antwerp, Belgium [3] Interuniversity Institute for Biostatistics and statistical Bioinformatics, Hasselt University, Belgium.
authors.forename: Dirk
authors.initials: D
authors.lastname: Valkenborg
authors.affiliation: 1] Unit Environmental Risk &Health, VITO, Mol, Belgium [2] Center for Proteomics, UAntwerp, Antwerp, Belgium.
authors.forename: Geert
authors.initials: G
authors.lastname: Baggerman
authors.affiliation: KU Leuven, Department of Biology, Laboratory of Neuroplasticity and Neuroproteomics, Leuven, Belgium.
authors.forename: Lutgarde
authors.initials: L
authors.lastname: Arckens
mesh_terms: D000818:Animals; D019921:Biotinylation; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D058977:Molecular Sequence Annotation; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D020543:Proteome / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome