title: Measurement of feline lipase activity using a dry-chemistry assay with a triolein substrate and comparison with pancreas-specific lipase (Spec fPL(TM)).
abstract: Pancreatic lipase immunoreactivity (Spec fPL) is currently considered to be the most accurate blood test for the diagnosis of feline pancreatitis. In this study, we measured lipase activity in cats using a newer catalytic lipase assay of dry-chemistry system (FDC-v-LIP) to determine the reference range and compared the results with those for Spec fPL. Based on the results of healthy cats, the reference range of FDC-v-LIP was determined to be less than 30 U/l. FDC-v-lip did not show a strong correlation with Spec fPL in cats with various diseases, which resulted in the low sensitivity and positive predictive value. However, the relatively high (>90%) specificity and negative predictive value indicated that FDC-v-LIP could be a useful patient-side screening test for the exclusion of feline pancreatitis.
authors.affiliation: Veterinary Medical Center and Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Oishi
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Toru
authors.initials: T
authors.lastname: Sato
authors.forename: Takashi
authors.initials: T
authors.lastname: Tamamoto
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanemoto
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000097:blood* / Q000175:diagnosis; D002415:Cats; D005260:Female; D008049:Lipase / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D010195:Pancreatitis / Q000097:blood / Q000662:veterinary*; D012016:Reference Values; D012680:Sensitivity and Specificity; D014304:Triolein / Q000737:chemistry*
chemical_list: D014304:Triolein; D008049:Lipase