title: Transmitted Extended-Spectrum Extensively Drug-Resistant Tuberculosis in Beijing, China, with Discordant Whole-Genome Sequencing Analysis Results.
abstract: Drug resistance to tuberculosis remains a major public health threat. Here, we report two cases of extended-spectrum extensively drug-resistant (XXDR) tuberculosis showing resistance to most first- and second-line agents. The results of a correlation of whole-genome sequencing (WGS) and phenotypic testing were discordant, suggesting that overreliance on WGS may miss clinically relevant resistance in extensively drug-resistant disease.
authors.affiliation: Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Masood ur Rehman
authors.initials: Mu
authors.lastname: Kayani
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
authors.forename: Yunting
authors.initials: Y
authors.lastname: Gu
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Duan
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Ma
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China huanghairong@tb123.org bjavid@gmail.com.
authors.forename: Hairong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University, Beijing, China huanghairong@tb123.org bjavid@gmail.com.
authors.forename: Babak
authors.identifier: http://orcid.org/0000-0002-6354-6305
authors.initials: B
authors.lastname: Javid
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology; D000068476:Beijing; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D054908:Extensively Drug-Resistant Tuberculosis / Q000175:diagnosis* / Q000635:transmission*; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial