title: DNA interaction of europium(III) complex containing 2,2'-bipyridine and its antimicrobial activity.
abstract: The interaction of native fish salmon DNA (FS-DNA) with [Eu(bpy)3Cl2(H2O)]Cl, where bpy is 2,2'-bipyridine, is studied at physiological pH in Tris-HCl buffer by spectroscopic methods, viscometric techniques as well as circular dichroism (CD). These experiments reveal that Eu(III) complex has interaction with FS-DNA. Moreover, binding constant and binding site size have been determined. The value of Kb has been defined 2.46 ± .02 × 10(5) M(-1). The thermodynamic parameters are calculated by Van't Hoff equation, the results show that the interaction of the complex with FS-DNA is an entropically driven phenomenon. CD spectroscopy followed by viscosity as well as fluorescence and UV--Vis measurements indicate that the complex interacts with FS-DNA via groove binding mode. Also, the synthesized Eu(III) complex has been screened for antimicrobial activities.
authors.affiliation: a Department of Chemistry , University of Sistan and Baluchestan , Zahedan , P.O. Box 98155-147, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Jahani
authors.affiliation: a Department of Chemistry , University of Sistan and Baluchestan , Zahedan , P.O. Box 98155-147, Iran.
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Khorasani-Motlagh
authors.affiliation: a Department of Chemistry , University of Sistan and Baluchestan , Zahedan , P.O. Box 98155-147, Iran.
authors.forename: Meissam
authors.initials: M
authors.lastname: Noroozifar
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014783:Viscosity
chemical_list: D000890:Anti-Infective Agents; D012997:Solvents; D005063:Europium; D015082:2,2'-Dipyridyl; D004247:DNA
keywords: 2,2′-bipyridine; DNA binding; antimicrobial; circular dichroism; europium(III) complex; viscometric techniques