title: Pharmacological substances in vitro in limiting growth and development of fungi Colletotrichum genera.
abstract: OBJECTIVE
  The aim of the study was to determine the antimycotic effect of selected substances, povidone iodine at various concentrations and fluconazole, on the growth and development of Colletotrichum spp., which is one of the ocular pathogens.
  
  
  METHODS
  The materials used for the study consisted of 1-spore cultures of 4 fungal species of the genus Colletotrichum: C. dematium, C. gloeosporioides, C. acutatum, and C. coccodes. The method of poisoning culture media and the method of stippling the substance onto fungal colonies were used in the study. Different concentrations of fluconazole (1%) and povidone iodine (1%, 2% and 5%) were evaluated.
  
  
  RESULTS
  The growth of the studied fungal species was inhibited in 100% on the medium containing povidone iodine at the concentration of 1%, 2%, and 5%. After 24 h from the application of povidone iodine, a local disappearance of aerial mycelium was observed. In the case of C. coccodes, the colonies were not damaged. After 24 h from the application of fluconazole on C. dematium, C. gloeosporioides and C. acutatum colonies, slight disappearance of aerial mycelium was observed at these points. Despite dispensing the substance during the next few days, the inhibitory effect did not increase. After the application fluconazole on the C. coccodes colonies, the inhibitory effect of the preparation was not observed.
  
  
  CONCLUSIONS
  The method of stippling of a preparation onto fungal colonies is a quick and reliable method to test many pharmacological substances. One percent, 2%, and 5% povidone iodine in culture medium is fungicidal for Colletotrichum spp. One percent fluconazole in culture medium is fungistatic for Colletotrichum spp. C. coccodes reveals the highest degree of insusceptibility to antimycotic treatment.
authors.affiliation: 1Department of Diagnostics and Microsurgery of Glaucoma, Medical University of Lublin, Lublin, Poland.
authors.forename: Eulalia
authors.initials: E
authors.lastname: Machowicz-Matejko
authors.affiliation: 2Department of Plant Pathology and Mycology, University of Life Sciences in Lublin, Lublin, Poland.
authors.forename: Ewa D
authors.initials: ED
authors.lastname: Zalewska
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage; D000935:Antifungal Agents / Q000494:pharmacology; D020231:Colletotrichum / Q000187:drug effects* / Q000254:growth & development* / Q000302:isolation & purification; D005128:Eye Diseases / Q000188:drug therapy / Q000382:microbiology; D015821:Eye Infections, Fungal / Q000188:drug therapy / Q000382:microbiology; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D011206:Povidone-Iodine / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D011206:Povidone-Iodine; D015725:Fluconazole