title: Proteomic Analysis of Duodenal Tissue from Escherichia coli F18-Resistant and -Susceptible Weaned Piglets.
abstract: Diarrhea and edema disease in weaned piglets due to infection by Escherichia coli F18 is a leading cause of economic loss in the pig industry. Resistance to E. coli F18 depends on expression of receptors on intestinal epithelial cells, and individual immunity. This study was conducted in Sutai pig E. coli F18-resistant and -susceptible full sib-pair individuals, identified on the basis of resource populations and verification of adhesion assays. The molecular mechanism underlying E. coli F18 resistance was investigated through analysis of the expression of E. coli F18 receptor associated and innate immunity proteins, using proteomics and bioinformatics techniques. Two-dimensional electrophoresis analysis revealed a total of 20 differentially expressed proteins in E. coli F18-resistant and -susceptible groups (10 upregulated and 10 downregulated). A total of 16 differentially expressed proteins were identified by MALDI TOF/TOF mass spectral analysis. According to gene ontology and pathway analysis, differentially expressed proteins were mainly involved in cell adhesion, immune response and other biologically relevant functions. Network analysis of interactions between differentially expressed proteins indicated a likelihood of their involvement in E. coli F18 infection. The expression levels of several important proteins including actin beta (ACTB), vinculin (VCL), heat stress proteins (HSPs) and transferrin (TF) in E. coli F18-resistant and -susceptible individuals were verified by Western blotting, supporting the identification of ACTB, VCL, HSPs and TF as promising candidate proteins for association with E. coli F18 susceptibility.
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.
authors.forename: Zhengchang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.
authors.forename: Riwei
authors.initials: R
authors.lastname: Xia
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Yin
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.
authors.forename: Yongjiu
authors.initials: Y
authors.lastname: Huo
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, P. R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.
authors.forename: Shenglong
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Bao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001422:Bacterial Adhesion; D060467:Disease Resistance; D004198:Disease Susceptibility; D004386:Duodenum / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D063990:Gene Ontology; D013058:Mass Spectrometry; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D034421:Sus scrofa / Q000382:microbiology*; D014886:Weaning