title: In situ characterizing membrane lipid phenotype of breast cancer cells using mass spectrometry profiling.
abstract: Lipid composition in cell membrane is closely associated with cell characteristics. Here, matrix-assisted laser desorption/ionization- Fourier transform ion cyclotron resonance mass spectrometry was employed to in situ determine membrane components of human mammary epithelial cells (MCF-10 A) and six different breast cancer cell lines (i.e., BT-20, MCF-7, SK-BR-3, MDA-MB-231, MDA-MB-157, and MDA-MB-361) without any lipid extraction and separation. Partial least-square discriminant analysis indicated that changes in the levels of these membrane lipids were closely correlated with the types of breast cell lines. Elevated levels of polyunsaturated lipids in MCF-10 A cells relative to six breast cancer cells and in BT-20 cells relative to other breast cancer cell lines were detected. The Western blotting assays indicated that the expression of five lipogenesis-related enzymes (i.e., fatty acid synthase 1(FASN1), stearoyl-CoA desaturase 1 (SCD1), stearoyl-CoA desaturase 5 (SCD5), choline kinase α (CKα), and sphingomyelin synthase 1) was associated with the types of the breast cells, and that the SCD1 level in MCF-7 cells was significantly increased relative to other breast cell lines. Our findings suggest that elevated expression levels of FASN1, SCD1, SCD5, and CKα may closely correlated with enhanced levels of saturated and monounsaturated lipids in breast cancer cell lines.
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P.R. China.
authors.forename: Manwen
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P.R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P.R. China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Li
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002797:Choline Kinase / Q000096:biosynthesis / Q000235:genetics; D054890:Fatty Acid Synthase, Type I / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D050155:Lipogenesis / Q000235:genetics; D061986:MCF-7 Cells; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013230:Stearoyl-CoA Desaturase / Q000096:biosynthesis / Q000235:genetics; D017855:Transferases (Other Substituted Phosphate Groups) / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C523776:SCD1 protein, human; C502659:SCD5 protein, human; D013230:Stearoyl-CoA Desaturase; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I; C517212:CHKA protein, human; D002797:Choline Kinase; C455440:SGMS1 protein, human; D017855:Transferases (Other Substituted Phosphate Groups)