title: Human psychophysics and rodent spinal neurones exhibit peripheral and central mechanisms of inflammatory pain in the UVB and UVB heat rekindling models.
abstract: Translational research is key to bridging the gaps between preclinical findings and the patients, and a translational model of inflammatory pain will ideally induce both peripheral and central sensitisation, more effectively mimicking clinical pathophysiology in some chronic inflammatory conditions. We conducted a parallel investigation of two models of inflammatory pain, using ultraviolet B (UVB) irradiation alone and UVB irradiation with heat rekindling. We used rodent electrophysiology and human quantitative sensory testing to characterise nociceptive processing in the peripheral and central nervous systems in both models. In both species, UVB irradiation produces peripheral sensitisation measured as augmented evoked activity of rat dorsal horn neurones and increased perceptual responses of human subjects to mechanical and thermal stimuli. In both species, UVB with heat rekindling produces central sensitisation. UVB irradiation alone and UVB with heat rekindling are translational models of inflammation that produce peripheral and central sensitisation, respectively. The predictive value of laboratory models for human pain processing is crucial for improving translational research. The discrepancy between peripheral and central mechanisms of pain is an important consideration for drug targets, and here we describe two models of inflammatory pain that involve ultraviolet B (UVB) irradiation, which can employ peripheral and central sensitisation to produce mechanical and thermal hyperalgesia in rats and humans. We use electrophysiology in rats to measure the mechanically- and thermally-evoked activity of rat spinal neurones and quantitative sensory testing to assess human psychophysical responses to mechanical and thermal stimulation in a model of UVB irradiation and in a model of UVB irradiation with heat rekindling. Our results demonstrate peripheral sensitisation in both species driven by UVB irradiation, with a clear mechanical and thermal hypersensitivity of rat dorsal horn neurones and enhanced perceptual responses of human subjects to both mechanical and thermal stimulation. Additional heat rekindling produces markers of central sensitisation in both species, including enhanced receptive field sizes. Importantly, we also showed a correlation in the evoked activity of rat spinal neurones to human thermal pain thresholds. The parallel results in rats and humans validate the translational use of both models and the potential for such models for preclinical assessment of prospective analgesics in inflammatory pain states.
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, UK.
authors.forename: Jessica
authors.initials: J
authors.lastname: O'Neill
authors.affiliation: Wolfson Institute of Biomedical Research, University College London, UK.
authors.forename: Shafaq
authors.initials: S
authors.lastname: Sikandar
authors.affiliation: Wolfson Wing, King's College London, UK.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: McMahon
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, UK.
authors.forename: Anthony H
authors.initials: AH
authors.lastname: Dickenson
mesh_terms: D000328:Adult; D000818:Animals; D002056:Burns / Q000523:psychology*; D006358:Hot Temperature; D006801:Humans; D006930:Hyperalgesia / Q000503:physiopathology / Q000523:psychology*; D007249:Inflammation / Q000503:physiopathology / Q000523:psychology; D008297:Male; D010146:Pain / Q000503:physiopathology / Q000523:psychology*; D058748:Pain Perception; D017288:Pain Threshold; D010812:Physical Stimulation; D020671:Posterior Horn Cells / Q000502:physiology*; D011601:Psychophysics; D017207:Rats, Sprague-Dawley; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult