title: Study on the molecular regulatory mechanism of MicroRNA-195 in the invasion and metastasis of colorectal carcinoma.
abstract: OBJECTIVE
  This study aims to identify the target gene of hsa-miR-195 and to research the molecular mechanism of hsa-miR-195 which is through its target genes in the colorectal cancer invasion and metastasis.
  
  
  METHODS
  We used biological informatics (RNAhybrid and Target Scan analysis database) to predict the target genes of hsa-miR-195. Collected colon cancer tissues from clinical colorectal cancer patients by surgical removal of the carcinoma and control tissues, and researched the expression of Bcl-2 in tissues by immunohistochemical. Next, Real-time PCR was used to research the expression of hsa-miR-195 in Caco-2 and NCM460 cell line. hsa -miR-195 Mimics was transient transfered to Caco-2 cells, western blot was used to analysis the expression changes of Bcl-2. To analysis the possibility that hsa-miR-195 can affect the invasive ability of tumor cells by Bcl-2, we transferred hsa-miR-195 Mimics and Bcl-2 expression plasmid, and used the cell invasion experiment to discusses hsa-miR-195 effect on the ability of tumor cell invasion.
  
  
  RESULTS
  the immunohistochemical results showed that, the semi-quantitative parameters for the Bcl-2: control by 0.89 ± 0.51, 6 colon cancer by 31 ± 0.79. The expression of has-miR-195 in Caco-2 is 0.39 ± 1.5 while the value in control is2.01 ± 0.2, **P < 0.01.
  
  
  CONCLUSIONS
  In colorectal cancer, has-miR-195 can promote cell apoptosis and inhibit the invasion and metastasis by inhibiting the expression of Bcl-2.
authors.affiliation: Department of Gastroenterology, Harrison International Peace Hospital NO. 180 East Renmin Road, Hengshui City, Hebei 053000, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Colorectal Surgery, China-Japan Union hospital of Jilin University No. 126 Xiantai Street, Changchun 130031, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department of Colorectal Surgery, China-Japan Union hospital of Jilin University No. 126 Xiantai Street, Changchun 130031, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
keywords: Bcl-2; Caco-2; MicroRNA-195; NCM460; colorectal cancer