title: Key immune cell cytokines have a significant role in the expansion of CD26 population of cord blood mononuclear cells.
abstract: BACKGROUND
  Umbilical cord blood expresses cluster of differentiation (CD) 26, a fraction of CD34 + cells, negatively regulating in vivo homing and engraftment of hematopoietic stem cells. CD26 is highly expressed in various cells such as HSCs, immune cells, fibroblasts, and epithelial cells. It has been shown that inhibition of the CD26 on CD34 + cells improve the efficiency of transplantation of hematopoietic stem and progenitor cells. This study aimed to investigate the effect of key immune cell cytokines on CD26 expansion.
  
  
  METHODS
  Cord blood mononuclear cells were cultured for 21 days using the stem cell factor, fetal liver tyrosine kinase 3 (Flt3) ligand (FL), interleukin (IL) 2, IL7, and IL15. Harvested cells were analyzed by flow cytometry at distinct time points.
  
  
  RESULTS
  Our results showed that utilization of IL7 significantly improved the expression of total CD26 + cells (8.6-fold higher). When either IL2 or IL15 were added to the culture, the expression also improved 2.5-fold. The IL2 and IL7 showed significant effect on the expansion of both the CD26 + and CD26 fractions of the CD34 + cells. However, the effects of IL15 on CD26 + and CD26 -expansion were similar.
  
  
  CONCLUSIONS
  Taken together, our data suggested that using IL7 causes higher proliferation of CD26 cells in comparison to that seen under other culture conditions.
authors.affiliation: a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Zeynab
authors.initials: Z
authors.lastname: Aliyari
authors.affiliation: c Tissue Engineering Research Group, Advanced Research School, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Khaziri
authors.affiliation: c Tissue Engineering Research Group, Advanced Research School, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Balal
authors.initials: B
authors.lastname: Brazvan
authors.affiliation: d Women's Reproductive Health Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Manizheh
authors.initials: M
authors.lastname: Saayah Melli
authors.affiliation: c Tissue Engineering Research Group, Advanced Research School, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Tayefi Nasrabadi
authors.affiliation: e Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Hojjatollah
authors.initials: H
authors.lastname: Nozad Charoudeh
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology*; D018819:Dipeptidyl Peptidase 4; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D016207:Cytokines; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4
keywords: CD26 cells; CD34 cell; cytokine; umbilical cord blood