title: Mammary cancer stem cells reinitiation assessment at the metastatic niche: the lung and bone.
abstract: Mammary cancer stem cells (MCSC) have been operationally defined as cells that re-form secondary tumors upon transplantation into immunodeficient mice. Building on this observation, it has also been suggested that MCSCs are responsible for metastasis as well as evasion and resistance to therapeutic treatments. MCSC reinitiating potential is usually tested by implantation of limited amounts of cells orthotopically or subcutaneously, yet this poorly recapitulates the metastatic niche where truly metastatic reinitiation will occur. Herein, we describe the implantation of small amounts of MCSC selected populations in the bone (intra tibiae injection) and the lung (intra thoracic injection) to test for their metastatic reinitiation capabilities.
authors.affiliation: Oncology Program, Institute for Research in Biomedicine (IRB-Barcelona), PBB52 Parc Científic de Barcelona. C/Baldiri i Reixac 10-12, 08028, Barcelona, Spain.
authors.forename: Marc
authors.initials: M
authors.lastname: Guiu
authors.forename: Enrique J
authors.initials: EJ
authors.lastname: Arenas
authors.forename: Sylwia
authors.initials: S
authors.lastname: Gawrzak
authors.forename: Milica
authors.initials: M
authors.lastname: Pavlovic
authors.forename: Roger R
authors.initials: RR
authors.lastname: Gomis
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary; D001943:Breast Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary; D051379:Mice; D057054:Molecular Imaging / Q000379:methods; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured; D059016:Tumor Microenvironment