title: Tracing CD34+ Stromal Fibroblasts in Palatal Mucosa and Periodontal Granulation Tissue as a Possible Cell Reservoir for Periodontal Regeneration.
abstract: The aim of the present research was to trace CD34+ stromal fibroblastic cells (CD34+ SFCs) in the palatal connective tissue harvested for muco-gingival surgical procedures and in granulation tissues from periodontal pockets using immunohistochemical and transmission electron microscopy. Immunohistochemical analysis targeted the presence of three antigens: CD31, α-smooth muscle actin (α-SMA), and CD34. In the palate, CD31 staining revealed a colored inner ring of the vessels representing the endothelium, α-SMA+ was located in the medial layer of the vasculature, and CD34 was intensely expressed by endothelial cells and artery adventitial cells (considered to be CD34+ SFCs). Granulation tissue showed the same pattern for CD31+ and α-SMA, but a different staining pattern for CD34. Ultrastructural examination of the palatal tissue highlighted perivascular cells with fibroblast-like characteristics and pericytes in close spatial relationship to endothelial cells. The ultrastructural evaluation of granulation tissue sections confirmed the presence of neovasculature and the inflammatory nature of this tissue. The present study traced the presence of CD34+ SFCs and of pericytes in the palatal connective tissue thus highlighting once more its intrinsic regenerative capabilities. The clinical and systemic factors triggering mobilization and influencing the fate of local CD34+SCFs and other progenitors are issues to be further investigated.
authors.affiliation: 1Department of Periodontology, Faculty of Dental Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,15 V. Babeş Street,400012 Cluj-Napoca,Romania.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Roman
authors.affiliation: 1Department of Periodontology, Faculty of Dental Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,15 V. Babeş Street,400012 Cluj-Napoca,Romania.
authors.forename: Emőke
authors.initials: E
authors.lastname: Páll
authors.affiliation: 3Department of Histology, Faculty of Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,6 L. Pasteur Street,400349 Cluj-Napoca,Romania.
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Mihu
authors.affiliation: 1Department of Periodontology, Faculty of Dental Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,15 V. Babeş Street,400012 Cluj-Napoca,Romania.
authors.forename: Adrian S
authors.initials: AS
authors.lastname: Petruţiu
authors.affiliation: 4Department of Molecular Biology and Biotechnologies, Faculty of Biology and Geology,Babeş-Bolyai University,5-7 Clinicilor Street,400006 Cluj-Napoca,Romania.
authors.forename: Lucian
authors.initials: L
authors.lastname: Barbu-Tudoran
authors.affiliation: 5Department of Oral Rehabilitation, Faculty of Dental Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,15 V. Babeş Street,400012 Cluj-Napoca,Romania.
authors.forename: Radu S
authors.initials: RS
authors.lastname: Câmpian
authors.affiliation: 6Department of Cell and Molecular Biology, Faculty of Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,6 L. Pasteur Street,400349 Cluj-Napoca,Romania.
authors.forename: Adrian
authors.initials: A
authors.lastname: Florea
authors.affiliation: 7Department of Pathology, Faculty of Medicine,Iuliu Haţieganu University of Medicine and Pharmacy,8 V. Babeş Street,400012 Cluj-Napoca,Romania.
authors.forename: Carmen
authors.initials: C
authors.lastname: Georgiu
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology*; D005881:Gingiva / Q000166:cytology / Q000502:physiology*; D006097:Granulation Tissue / Q000254:growth & development*; D006801:Humans; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D009061:Mouth Mucosa / Q000166:cytology / Q000502:physiology*; D010159:Palate / Q000166:cytology / Q000502:physiology*; D020286:Pericytes / Q000737:chemistry / Q000502:physiology; D012038:Regeneration
chemical_list: D018952:Antigens, CD34
keywords: CD34 antigen; immunohistochemistry; pericytes; tissue graft; ultrastructure