title: Effect of lipo-chitooligosaccharide on early growth of C4 grass seedlings.
abstract: Although lipo-chitooligosaccharides (LCOs) are important signal molecules for plant-symbiont interactions, a number of reports suggest that LCOs can directly impact plant growth and development, separate from any role in plant symbioses. In order to investigate this more closely, maize and Setaria seedlings were treated with LCO and their growth was evaluated. The data indicate that LCO treatment significantly enhanced root growth. RNA-seq transcriptomic analysis of LCO-treated maize roots identified a number of genes whose expression was significantly affected by the treatment. Among these genes, some LCO-up-regulated genes are likely involved in root growth promotion. Interestingly, some stress-related genes were down-regulated after LCO treatment, which might indicate reallocation of resources from defense responses to plant growth. The promoter activity of several LCO-up-regulated genes using a β-glucuronidase reporter system was further analysed. The results showed that the promoters were activated by LCO treatment. The data indicate that LCO can directly impact maize root growth and gene expression.
authors.affiliation: Divisions of Plant Science and Biochemistry University of Missouri, Columbia, MO 65211, USA Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Divisions of Plant Science and Biochemistry University of Missouri, Columbia, MO 65211, USA Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Plant Transformation Core Facility,Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Divisions of Plant Science and Biochemistry University of Missouri, Columbia, MO 65211, USA Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: An Q
authors.initials: AQ
authors.lastname: Pham
authors.affiliation: Divisions of Plant Science and Biochemistry University of Missouri, Columbia, MO 65211, USA Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Josef M
authors.initials: JM
authors.lastname: Batek
authors.affiliation: Department of Statistics, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Cui
authors.affiliation: Department of Statistics, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA Informatics Institute, Department of Computer Science, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Saad M
authors.initials: SM
authors.lastname: Khan
authors.affiliation: Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA Informatics Institute, Department of Computer Science, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Trupti
authors.initials: T
authors.lastname: Joshi
authors.affiliation: Plant Transformation Core Facility,Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Zhanyuan J
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA Informatics Institute, Department of Computer Science, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.affiliation: Divisions of Plant Science and Biochemistry University of Missouri, Columbia, MO 65211, USA Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA staceyg@missouri.edu.
authors.forename: Gary
authors.initials: G
authors.lastname: Stacey
mesh_terms: D002244:Carbon / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D009844:Oligosaccharides; D006109:Poaceae / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects; D003313:Zea mays / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides; C493484:oligochitosan; D002686:Chitin; D002244:Carbon; D048271:Chitosan
keywords: Lipo-chitooligosaccharide; RNA-seq.; maize; non-legume; root growth promotion