title: Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation.
abstract: Nuf2 plays an important role in kinetochore-microtubule attachment and thus is involved in regulation of the spindle assembly checkpoint in mitosis. In this study, we examined the localization and function of Nuf2 during mouse oocyte meiotic maturation. Myc6-Nuf2 mRNA injection and immunofluorescent staining showed that Nuf2 localized to kinetochores from germinal vesicle breakdown to metaphase I stages, while it disappeared from the kinetochores at the anaphase I stage, but relocated to kinetochores at the MII stage. Overexpression of Nuf2 caused defective spindles, misaligned chromosomes, and activated spindle assembly checkpoint, and thus inhibited chromosome segregation and metaphase-anaphase transition in oocyte meiosis. Conversely, precocious polar body extrusion was observed in the presence of misaligned chromosomes and abnormal spindle formation in Nuf2 knock-down oocytes, causing aneuploidy. Our data suggest that Nuf2 is a critical regulator of meiotic cell cycle progression in mammalian oocytes.
authors.affiliation: a Institute of Reproductive Sciences; College of Animal Science and Technology; Qingdao Agricultural University ; Qingdao , China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Qi
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Wang
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Qian
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000782:Aneuploidy; D000818:Animals; D018797:Cell Cycle Proteins / Q000502:physiology*; D002478:Cells, Cultured; D002842:Chromatids / Q000502:physiology; D020090:Chromosome Segregation; D015870:Gene Expression; D008540:Meiosis; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000502:physiology*; D059705:Polar Bodies / Q000502:physiology; D021381:Protein Transport
chemical_list: D018797:Cell Cycle Proteins; C000607843:Nuf2 protein, mouse
keywords: Nuf2; kinetochore-microtubule attachments; meiosis; oocyte; spindle assembly checkpoint