title: Assessment of postoperative adhesion formation in a rat cecum model using different techniques.
abstract: Adhesion-related complications after abdominal surgery bring out momentous morbidity and costs. Outcomes from animal experiments investigating prevention of adhesions are limited due to lack of consistency in existing animal models. Different intraperitoneal adhesion models were compared the inter observer variability was evaluated to seek for best model. Forty male SD rats weighting 250-300g were included and assigned randomly to four groups with diverse techniques, (A) postoperative adhesion cecum rat model abraded with sterile rasp; (B) postoperative adhesion cecum rat model abraded with sterile dry gauze; (C) postoperative adhesion cecum rat model abraded with sterile blade; (D) postoperative adhesion cecum rat model abraded with vascular clamp. Macroscopic adhesion scores were evaluated by Bigatti scoring system, and the incidence of adhesion were surveyed on the 7th day after the surgery. The results showed that four techniques currently used Bigatti adhesion scoring system are subjective, the sterile rasp is the most consistent and reproducible tool to establish an intraperitoneal adhesion model which is helpful for related studies and the development of new substances for adhesion prevention in the future.
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Suzhou Hospital of Traditional Chinese Medicine, Suzhou, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yan
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Wen-lin
authors.initials: WL
authors.lastname: Li
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Suzhou Hospital of Traditional Chinese Medicine, Suzhou, China.
authors.forename: Yin-zi
authors.initials: YZ
authors.lastname: Yue
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Chen-xue
authors.initials: CX
authors.lastname: Jiang
authors.affiliation: Simcere Pharmaceutical Group, Nanjing, China.
authors.forename: Quan-fu
authors.initials: QF
authors.lastname: Feng
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Yao-yao
authors.initials: YY
authors.lastname: Bian
mesh_terms: D000818:Animals; D002432:Cecum / Q000601:surgery*; D013505:Digestive System Surgical Procedures / Q000379:methods*; D004195:Disease Models, Animal; D008297:Male; D010532:Peritoneal Diseases / Q000209:etiology* / Q000473:pathology; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013997:Time Factors; D000267:Tissue Adhesions