title: Cationic star copolymers based on β-cyclodextrins for efficient gene delivery to mouse embryonic stem cell colonies.
abstract: A cationic star copolymer with a β-cyclodextrin core was developed for nonviral gene transfer to mouse embryonic stem cells (mESCs). The copolymer comprises poly(2-dimethyl aminoethyl methacrylate) as the cationic component and poly(2-hydroxyethyl methacrylate) as the non-toxic stealth component. These materials have very low toxicity and show highly efficient transfection to mESC colonies.
authors.affiliation: Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602, Singapore. lohxj@imre.a-star.edu.sg.
authors.forename: Xian Jun
authors.initials: XJ
authors.lastname: Loh
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Wu
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D014162:Transfection; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D002412:Cations; D011108:Polymers; D047392:beta-Cyclodextrins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins