title: Chemerin Expression in the Peritoneal Fluid, Serum, and Ovarian Endometrioma of Women with Endometriosis.
abstract: OBJECTIVE
  Inflammation is an essential process in the pathogenesis of endometriosis.
  
  
  METHODS
  Serum and peritoneal fluid (PF) samples were collected from women with endometriosis (n = 31) and women without endometriosis (n = 48). Chemerin, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels in serum and PF samples were determined with a specific enzyme-linked immunosorbent assay. Eutopic endometrial tissue from controls and ovarian endometriotic cysts were obtained during surgery. Expression of chemerin and chemerin receptors in ectopic and eutopic endometrial tissues was measured on real-time reverse transcriptase-polymerase chain reaction. Protein expression was examined with Western blot and densitometric analysis.
  
  
  RESULTS
  Chemerin concentrations were higher in PF from women with endometriosis than that in that from controls. PF chemerin concentrations were significantly correlated with both TNF-α and IL-6 levels in PF. The mRNA and protein of chemerin and its receptor were significantly increased in the ovarian endometrioma tissue compared with eutopic endometrium of controls.
  
  
  CONCLUSIONS
  These findings suggest that chemerin plays a role in endometriosis-related pelvic inflammation.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Eulji University, Daejeon, Korea.
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Jin
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Kyong Wook
authors.initials: KW
authors.lastname: Yi
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Ha
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Hyun Tae
authors.initials: HT
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Tak
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Hur
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D018925:Chemokines / Q000096:biosynthesis / Q000097:blood*; D004715:Endometriosis / Q000276:immunology / Q000473:pathology*; D004717:Endometrium / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: C102741:CMKLR1 protein, human; D018925:Chemokines; C508600:IL6 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; C478630:RARRES2 protein, human; D019707:Receptors, Chemokine; D014409:Tumor Necrosis Factor-alpha
keywords: Chemerin; endometriosis; inflammation