title: Molecular epidemiology of an outbreak of hand, foot, and mouth disease associated with subgenotype C4a of enterovirus A71 in Nanchang, China in 2014.
abstract: An outbreak of hand, foot, and mouth disease was reported through hospital-based surveillance in Nanchang, China in 2014. A total of 244 cases were reported, 176 (72.1%) cases were tested positive for enteroviruses by direct reverse transcription-polymerase chain reaction, in which enterovirus A71 (EV-A71), coxsackievirus A16 (CV-A16), and untyped enteroviruses (UEV) accounted for 84.1%, 3.4%, and 12.5%, respectively. In this outbreak, children under 5 years old constituted more than 98% of the positive cases, and the ratio of male to female cases was 2.6 to 1 (P < 0.01). Phylogenetic analysis indicated that the Nanchang EV-A71 strains belonged to subgenotype C4a undergoing continuously evolutionary changes.
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Infectious Diseases, Children's Hospital of Jiangxi Province, Nanchang, China.
authors.forename: Qingxiong
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xia
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Fenglan
authors.initials: F
authors.lastname: He
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Maohong
authors.initials: M
authors.lastname: Hu
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Ni
authors.affiliation: Nanchang University Medical College, Nanchang, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Gao
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Chen
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China.
authors.forename: Shengen
authors.initials: S
authors.lastname: Chen
mesh_terms: D017677:Age Distribution; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D029821:Enterovirus A, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D005260:Female; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral
keywords: enterovirus A71; foot; hand; molecular epidemiology; mouth disease; subgenotype C4a