title: Synthesis of DNA fragments containing 2'-deoxy-4'-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4'-position in replication.
abstract: 4'-SelenoDNA fragments were synthesized for the first time using 4'-selenothymidine triphosphate (SeTTP) by taking advantage of its bioequivalence against DNA polymerases. DNA fragments each with a homologous element (O, S or Se) at the 4'-position of the thymidine units were effectively amplified using KOD Dash DNA polymerase.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi 1-78-1, Tokushima 770-8505, Japan. minakawa@tokushima-u.ac.jp.
authors.forename: N
authors.initials: N
authors.lastname: Tarashima
authors.forename: T
authors.initials: T
authors.lastname: Sumitomo
authors.forename: H
authors.initials: H
authors.lastname: Ando
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: N
authors.initials: N
authors.lastname: Minakawa
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008958:Models, Molecular; D016566:Organoselenium Compounds / Q000737:chemistry* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: C531225:4'-selenothymidine; D016566:Organoselenium Compounds; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine