title: An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier.
abstract: A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
authors.affiliation: Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 689-798, Korea. sabsab7@unist.ac.kr.
authors.forename: Jisu
authors.initials: J
authors.lastname: Lee
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Song
authors.forename: Yoonji
authors.initials: Y
authors.lastname: Bae
authors.forename: Junseon
authors.initials: J
authors.lastname: Min
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Rhee
authors.forename: Tae Joo
authors.initials: TJ
authors.lastname: Park
authors.forename: Moonil
authors.initials: M
authors.lastname: Kim
authors.forename: Sebyung
authors.initials: S
authors.lastname: Kang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D060387:Ascorbate Peroxidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D017434:Protein Structure, Tertiary; D058525:Quartz Crystal Microbalance Techniques; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D020349:Surface Plasmon Resonance
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases