title: Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma.
abstract: The present study aimed to identify serum biomarkers for the detection of hepatoblastoma (HB). Serum samples were collected from 71 HB patients (stage I, n = 19; stage II, n = 19, stage III, n = 19; and stage IV, n = 14) and 23 age- and sex-matched healthy children. Differential expression of serum protein markers were screened using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), and the target proteins were isolated and purified using HPLC and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), SEQUEST, and bioinformatics analysis. Differential protein expression was confirmed by enzyme-linked immunosorbent analysis (ELISA). SELDI-TOF-MS screening identified a differentially expressed protein with an m/z of 9348 Da, which was subsequently identified as Apo A-I; its expression was significantly lower in the HB group as compared to the normal control group (1546.67 ± 757.81 vs. 3359.21 ± 999.36, respectively; p < 0.01). Although the expression level decreased with increasing disease stage, pair-wise comparison revealed significant differences in Apo A-I expression between the normal group and the HB subgroups (p < 0.01). ELISA verified the reduced expression of Apo A-I in the HB group. Taken together, these results suggest that Apo A-I may represent a serum protein biomarker of HB. Further studies will assess the value of using Apo A-I expression for HB diagnosis and staging.
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. 13460300188@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. lijuan19881027@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. zhangjunjiezzu@126.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. gaopengfeizzu@126.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gao
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. peihangzzu@163.com.
authors.forename: Hang
authors.initials: H
authors.lastname: Pei
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. wangleizzuff@126.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. guofeizzu@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Institute of Cancer, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Jiefang Road, Hangzhou 310000, China. yujiekaizju@163.com.
authors.forename: Jiekai
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Cancer, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Jiefang Road, Hangzhou 310000, China. zhengshuzju@163.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China. wjiaxiang@zzu.edu.cn.
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018197:Hepatoblastoma / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins
keywords: 2D-LC-LTQ-MS; MALDI-TOF-MS; SELDI-TOF-MS; apolipoprotein A–I; hepatoblastom; protein biomarker