title: Long noncoding RNA HIF1A-AS1A reduces apoptosis of vascular smooth muscle cells: implications for the pathogenesis of thoracoabdominal aorta aneurysm.
abstract: Long non-coding RNAs (IncRNAs) play important roles in various biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little is known about the role of IncRNA HIF 1 alpha-antisense RNA 1 (HIF1a-AS1) in regulating the proliferation and apoptosis of vascular smooth muscle cells (VSMCs) and the expression of HIF1a-AS1 in serum of thoracoabdominal aortic aneurysm (TAAA) patients. The cell viability was detected by the CCK8 assay. The cell apoptosis was assessed by annexin V-PI double-labeling staining. Expression of genes and proteins were analyzed by real-time PCR and western blotting, respectively. Cells were transfected with siRNAs as a gene silencing method. In serum of TAAA patients, the expression of HIF1a-AS1 was significantly increased (superior to 6-fold) compared to the normal control. Moreover, Palmitic acid (PA) induced cell apoptosis in VSMCs in a time- and dose-dependent manner, and the proportion of the apoptotic cells had gained as compared to untreatment group. PA also induced up-regulation expression of HIF1a-AS1. We also found that transfection of cells with HIF1a-AS1 siRNA decreased the expression of caspase-3 and caspase-8 and increased the expression of Bcl2, and protected PA-induced cell apoptosis in VSMCs. HIF1a-AS1 was overexpressed in the TAAA and the interaction between HIF1a-AS1 and apoptotic proteins plays a key role in the proliferation and apoptosis of VSMCs in vitro, which may contribute to the pathogenesis of TAAA.
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.forename: Jinyun
authors.initials: J
authors.lastname: Tan
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Weihao
authors.initials: W
authors.lastname: Shi
authors.forename: Kun
authors.initials: K
authors.lastname: Liang
mesh_terms: D001012:Aorta, Abdominal; D001013:Aorta, Thoracic; D017545:Aortic Aneurysm, Thoracic / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D034622:RNA Interference; D016372:RNA, Antisense / Q000494:pharmacology*; D062085:RNA, Long Noncoding / Q000494:pharmacology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D016372:RNA, Antisense; D062085:RNA, Long Noncoding