title: Effect of orally administered collagen hydrolysate on gene expression profiles in mouse skin: a DNA microarray analysis.
abstract: Dietary collagen hydrolysate has been hypothesized to improve skin barrier function. To investigate the effect of long-term collagen hydrolysate administration on the skin, we evaluated stratum corneum water content and skin elasticity in intrinsically aged mice. Female hairless mice were fed a control diet or a collagen hydrolysate-containing diet for 12 wk. Stratum corneum water content and skin elasticity were gradually decreased in chronologically aged control mice. Intake of collagen hydrolysate significantly suppressed such changes. Moreover, we used DNA microarrays to analyze gene expression in the skin of mice that had been administered collagen hydrolysate. Twelve weeks after the start of collagen intake, no significant differences appeared in the gene expression profile compared with the control group. However, 1 wk after administration, 135 genes were upregulated and 448 genes were downregulated in the collagen group. This suggests that gene changes preceded changes of barrier function and elasticity. We focused on several genes correlated with functional changes in the skin. Gene Ontology terms related to epidermal cell development were significantly enriched in upregulated genes. These skin function-related genes had properties that facilitate epidermal production and differentiation while suppressing dermal degradation. In conclusion, our results suggest that altered gene expression at the early stages after collagen administration affects skin barrier function and mechanical properties. Long-term oral intake of collagen hydrolysate improves skin dysfunction by regulating genes related to production and maintenance of skin tissue.
authors.affiliation: Food Science Research Labs, Meiji Company, Limited, Naruda, Odawara, Kanagawa, Japan; chisato.ohba@meiji.com.
authors.forename: Chisato
authors.initials: C
authors.lastname: Oba
authors.affiliation: Food Science Research Labs, Meiji Company, Limited, Naruda, Odawara, Kanagawa, Japan;
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ito
authors.affiliation: Food Science Research Labs, Meiji Company, Limited, Naruda, Odawara, Kanagawa, Japan;
authors.forename: Satomi
authors.initials: S
authors.lastname: Ichikawa
authors.affiliation: Food Science Research Labs, Meiji Company, Limited, Naruda, Odawara, Kanagawa, Japan;
authors.forename: Masashi
authors.initials: M
authors.lastname: Morifuji
authors.affiliation: Institute for Food Sciences, Hirosaki University, Yanagawa, Aomori, Japan; and.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishijima
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.affiliation: Food Science Research Labs, Meiji Company, Limited, Naruda, Odawara, Kanagawa, Japan;
authors.forename: Keiko
authors.initials: K
authors.lastname: Kawahata
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016000:Cluster Analysis; D003094:Collagen / Q000008:administration & dosage* / Q000494:pharmacology*; D020405:Dermis / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004548:Elasticity; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005399:Fishes; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006868:Hydrolysis; D008812:Mice, Hairless; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D003094:Collagen
keywords: DNA microarray; collagen hydrolysate; epidermal barrier function; skin aging; skin elasticity