title: Tauroursodeoxycholic acid improves the implantation and live-birth rates of mouse embryos.
abstract: We previously demonstrated that tauroursodeoxycholic acid (TUDCA) improved the developmental competence of mouse embryos by attenuating endoplasmic reticulum (ER) stress-induced apoptosis during preimplantation development. Here, we present a follow-up study examining whether TUDCA enhances the implantation and live-birth rate of mouse embryos. Mouse 2-cell embryos were collected by oviduct flushing and cultured in the presence or absence of 50 μM TUDCA. After culture (52 h), blastocysts were transferred to 2.5-day pseudopregnant foster mothers. It was found that the rates of pregnancy and implantation as well as the number of live pups per surrogate mouse were significantly higher in the TUDCA-treated group compared to the control group, but there was no significant difference in the mean weights of the pups or placentae. Thus, we report for the first time that TUDCA supplementation of the embryo culture medium increased the implantation and livebirth rates of transferred mouse embryos.
authors.affiliation: Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Tao
authors.initials: T
authors.lastname: Lin
authors.affiliation: Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Yun Fei
authors.initials: YF
authors.lastname: Diao
authors.affiliation: Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Kang
authors.affiliation: Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jae Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Dong Kyu
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea. Electronic address: dijin@cnu.ac.kr.
authors.forename: Dong Il
authors.initials: DI
authors.lastname: Jin
mesh_terms: D000818:Animals; D001724:Birth Weight / Q000187:drug effects; D001755:Blastocyst / Q000187:drug effects; D001757:Blastomeres / Q000187:drug effects*; D003433:Crosses, Genetic; D004477:Ectogenesis / Q000187:drug effects*; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D005300:Fertility Agents, Female / Q000009:adverse effects / Q000494:pharmacology*; D008098:Litter Size / Q000187:drug effects; D050498:Live Birth; D008297:Male; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D009994:Osmolar Concentration; D010929:Placentation / Q000187:drug effects; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted / Q000295:instrumentation; D013655:Taurochenodeoxycholic Acid / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D005300:Fertility Agents, Female; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine
keywords: Embryo transfer; Implantation; Mouse embryo; TUDCA