title: Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel.
abstract: Western blotting enables the detection and characterization of proteins of low abundance. Sodium dodecyl sulfate (SDS) polyacrylamide gel-separated proteins are normally transferred electrophoretically to nitrocellulose or polyvinylidene difluoride membranes. Here we describe the transfer proteins [Ro 60 (or SSA) autoantigen, 220 and 240 kDa spectrin antigens, and prestained molecular weight standards] by diffusion from SDS polyacrylamide gels at 37 °C. Up to 12 immunoblots can be obtained from a single gel by this method.
authors.affiliation: Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA, biji-kurien@omrf.org.
authors.forename: Biji T
authors.initials: BT
authors.lastname: Kurien
authors.forename: R Hal
authors.initials: RH
authors.lastname: Scofield
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004058:Diffusion; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000941:Antigens; D005782:Gels; D011506:Proteins