title: Polymerase Chain Reaction on a Viral Nanoparticle.
abstract: The field of synthetic biology includes studies that aim to develop new materials and devices from biomolecules. In recent years, much work has been carried out using a range of biomolecular chassis including α-helical coiled coils, β-sheet amyloids and even viral particles. In this work, we show how hybrid bionanoparticles can be produced from a viral M13 bacteriophage scaffold through conjugation with DNA primers that can template a polymerase chain reaction (PCR). This unprecedented example of a PCR on a virus particle has been studied by flow aligned linear dichroism spectroscopy, which gives information on the structure of the product as well as a new protototype methodology for DNA detection. We propose that this demonstration of PCR on the surface of a bionanoparticle is a useful addition to ways in which hybrid assemblies may be constructed using synthetic biology.
authors.forename: James
authors.initials: J
authors.lastname: Carr-Smith
authors.forename: Raúl
authors.initials: R
authors.lastname: Pacheco-Gómez
authors.forename: Haydn A
authors.initials: HA
authors.lastname: Little
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Hicks
authors.forename: Sandeep
authors.initials: S
authors.lastname: Sandhu
authors.forename: Nadja
authors.initials: N
authors.lastname: Steinke
authors.forename: David J
authors.initials: DJ
authors.lastname: Smith
authors.affiliation: Department of Chemistry, University of Warwick , Coventry, Warwickshire CV4 7AL, United Kingdom.
authors.forename: Alison
authors.initials: A
authors.lastname: Rodger
authors.affiliation: Defence Science and Technology Laboratory , Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Goodchild
authors.affiliation: Defence Science and Technology Laboratory , Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom.
authors.forename: Roman A
authors.initials: RA
authors.lastname: Lukaszewski
authors.forename: James H R
authors.initials: JH
authors.lastname: Tucker
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Dafforn
mesh_terms: D017104:Bacteriophage M13 / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers
keywords: M13; PCR; bacteriophage; linear dichroism; nanoparticle; shear flow