title: Fermentative Production of Pyranone Derivate I from Marine Vibrio sp. SKMARSP9: Isolation, Characterization and Bioactivity Evaluation.
abstract: Pyranone derivative I was isolated from fermented broth of isolated marine bacterial strain Vibrio sp. SKMARSP9. The compound I was characterized, and evaluated for its antimicrobial properties. The isolated strain was identified based on 16S rRNA based phylogenetic analysis. The molecular analysis data suggested that this strain is closely related to Vibrio ruber, Vibrio sp. MSSRF10 and Vibrio rhizosphaerae. The best fermentative growth of this isolate was achieved under halophilic conditions and grew efficiently at 30 °C in the presence of 12 % NaCl. The compound I production by this strain is associated with growth. The unpurified extract is hydrophobic in nature, and released only during late growth phase. The extract was purified and characterized by spectral data using NMR, DEPT, and ESI-MS. The purity of I was 97 % which was confirmed by HPLC. The pyranone derivative I exhibited >50 % antioxidant activity and broad spectrum antimicrobial properties against gram negative and gram positive strains. Molecular docking analysis revealed that this pyranone derivative I may be a potential candidate at pharmaceutical sector.
authors.affiliation: Department of Microbiology, Kakatiya University, Warangal, 506009 India.
authors.forename: P
authors.initials: P
authors.lastname: Shiva Krishna
authors.affiliation: Bio Engineering & Environmental Sciences, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007 India.
authors.forename: B
authors.initials: B
authors.lastname: Sudheer Kumar
authors.affiliation: Medicinal Chemistry & Pharmacology, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007 India.
authors.forename: P
authors.initials: P
authors.lastname: Raju
authors.affiliation: Medicinal Chemistry & Pharmacology, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007 India.
authors.forename: M S R
authors.initials: MS
authors.lastname: Murty
authors.affiliation: Centre for NMR & Structural Chemistry, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007 India.
authors.forename: T
authors.initials: T
authors.lastname: Prabhakar Rao
authors.affiliation: Department of Microbiology, Kakatiya University, Warangal, 506009 India.
authors.forename: M A
authors.initials: MA
authors.lastname: Singara Charya
authors.affiliation: Bio Engineering & Environmental Sciences, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007 India.
authors.forename: R S
authors.initials: RS
authors.lastname: Prakasham
keywords: Antibacterial activity; Antioxidant activity; ESI–MS; NMR; Pyranone derivative