title: Composite SERS-based satellites navigated by optical tweezers for single cell analysis.
abstract: Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.
authors.affiliation: Saratov State University, 410012, Saratov, Russia. gorinda@mail.ru.
authors.forename: Inna Y
authors.initials: IY
authors.lastname: Stetciura
authors.forename: Alexey
authors.initials: A
authors.lastname: Yashchenok
authors.forename: Admir
authors.initials: A
authors.lastname: Masic
authors.forename: Evgeny V
authors.initials: EV
authors.lastname: Lyubin
authors.forename: Olga A
authors.initials: OA
authors.lastname: Inozemtseva
authors.forename: Maria G
authors.initials: MG
authors.lastname: Drozdova
authors.forename: Elena A
authors.initials: EA
authors.lastname: Markvichova
authors.forename: Boris N
authors.initials: BN
authors.lastname: Khlebtsov
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Fedyanin
authors.forename: Gleb B
authors.initials: GB
authors.lastname: Sukhorukov
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Gorin
authors.forename: Dmitry
authors.initials: D
authors.lastname: Volodkin
mesh_terms: D000818:Animals; D002460:Cell Line; D004867:Equipment Design; D005347:Fibroblasts / Q000166:cytology*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D052898:Optical Tweezers; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D059010:Single-Cell Analysis / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*
chemical_list: D012834:Silver; D006046:Gold; D012822:Silicon Dioxide