title: Simultaneous separation and sensitive detection of naringin and naringenin in nanoparticles by chromatographic method indicating stability and photodegradation kinetics.
abstract: A simple, sensitive, precise and linear method by liquid chromatography was established for simultaneous determination and quantification of naringin and naringenin in polymeric nanoparticles. The method results in excellent separation in <11 min and with a peak purity of both flavonoids. The analyses were performed using a C18 column (4.6 × 150 mm, 5 µm), at a 1 mL/min flow rate. The mobile phase consisted of a gradient of acetonitrile-water (pH 4.0; v/v) at a temperature of 25°C. The nanoparticles were prepared according to the method of interfacial deposition of a pre-formed polymer. The method were validated in compliance with guidelines, and was found to be linear in the 1-40 µg/mL concentration range for both naringin and naringenin (r > 0.99). Repeatability was determined at three concentration levels, obtaining an RSD (%) <0.9%, and the accuracy of the method was >98%. The photodegradation kinetics was determined for naringin; the coefficient that best represents degradation was of first order and naringenin presented a zero-order kinetics. To our knowledge, a rapid and sensitive method for naringin and naringenin in polymeric nanoparticles has not been published elsewhere and this method is applicable to simultaneous evaluation of flavonoids.
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Porto Alegre, RS, Brazil.
authors.forename: Leticia Malgarim
authors.initials: LM
authors.lastname: Cordenonsi
authors.affiliation: Programa de Pós Graduação em Nanociências, Centro Universitário Franciscano, 97010-032, Santa Maria, RS, Brazil.
authors.forename: Nathany Genro
authors.initials: NG
authors.lastname: Bromberger
authors.affiliation: Programa de Pós Graduação em Nanociências, Centro Universitário Franciscano, 97010-032, Santa Maria, RS, Brazil.
authors.forename: Renata Platcheck
authors.initials: RP
authors.lastname: Raffin
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Porto Alegre, RS, Brazil.
authors.forename: Elfrides Eva
authors.initials: EE
authors.lastname: Scherman
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D044950:Flavanones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D010782:Photolysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D044950:Flavanones; C005273:naringenin; C005274:naringin
keywords: HPLC validation; naringenin; naringin; stability