title: Yeast red pigment modifies Amyloid beta growth in Alzheimer disease models in both Saccharomyces cerevisiae and Drosophila melanogaster.
abstract: The effect of yeast red pigment on amyloid-β (Aβ) aggregation and fibril growth was studied in yeasts, fruit flies and in vitro. Yeast strains accumulating red pigment (red strains) contained less amyloid and had better survival rates compared to isogenic strains without red pigment accumulation (white strains). Confocal and fluorescent microscopy was used to visualise fluorescent Aβ-GFP aggregates. Yeast cells containing less red pigment had more Aβ-GFP aggregates despite the lower level of overall GFP fluorescence. Western blot analysis with anti-GFP, anti-Aβ and A11 antibodies also revealed that red cells contained a considerably lower amount of Aβ GFP aggregates as compared to white cells. Similar results were obtained with exogenous red pigment that was able to penetrate yeast cells. In vitro experiments with thioflavine and TEM showed that red pigment effectively decreased Aβ fibril growth. Transgenic flies expressing Aβ were cultivated on medium containing red and white isogenic yeast strains. Flies cultivated on red strains had a significant decrease in Aβ accumulation levels and brain neurodegeneration. They also demonstrated better memory and learning indexes and higher locomotor ability.
authors.affiliation: Institute of Cytology of RAS , St. Petersburg , Russia .
authors.forename: Olga V
authors.initials: OV
authors.lastname: Nevzglyadova
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Mikhailova
authors.forename: Triana R
authors.initials: TR
authors.lastname: Amen
authors.forename: Valeriy V
authors.initials: VV
authors.lastname: Zenin
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Artemov
authors.forename: Elena I
authors.initials: EI
authors.lastname: Kostyleva
authors.forename: Daria A
authors.initials: DA
authors.lastname: Mezhenskaya
authors.forename: Dmitry I
authors.initials: DI
authors.lastname: Rodin
authors.forename: Alsu F
authors.initials: AF
authors.lastname: Saifitdinova
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Khodorkovskii
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Sarantseva
authors.forename: Tonu R
authors.initials: TR
authors.lastname: Soidla
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000473:pathology; D004331:Drosophila melanogaster; D005434:Flow Cytometry; D009043:Motor Activity / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments
keywords: Alzheimer disease; Aβ; amyloid; neurodegeneration; yeast red pigment