title: Human Surfactant Proteins A2 (SP-A2) and B (SP-B) Genes as Determinants of Respiratory Distress Syndrome.
abstract: OBJECTIVE
  To study the relationship between SP-A2 and SP-B gene polymorphisms and respiratory distress syndrome in preterm neonates.
  
  
  METHODS
  Cross-sectional.
  
  
  METHODS
  Neonatal intensive care unit and the Molecular Biology unit of the Chemical Pathology Department, Kasr Alainy hospital, Cairo University.
  
  
  METHODS
  Sixty-five preterm infants with respiratory distress syndrome and 50 controls. The genomic DNA was isolated using DNA extraction kits. SYBR Green-based real-time PCR was used to determine the variant genotypes of SP-A2 c.751 G>A and SP-B c.8714 G>C single nucleotide polymorphisms.
  
  
  RESULTS
  Homozygosity of SP-A (OR 46, 95% CI 14-151) and SP-B (OR 5.2, 95% CI 2.3-11.4) alleles increased the risk of respiratory distress syndrome. The logistic regression model showed that genotypes SP-A2 (OR 164) and SP-B (OR 18) were directly related to the occurrence of respiratory distress syndrome, whereas gestational age (OR 0.57) and 5-minute Apgar score (OR 0.19) were inversely related to its occurrence.
  
  
  CONCLUSIONS
  There is a possible involvement of SP-A2 and SP-B genes polymorphisms in the genetic predisposition to respiratory distress syndrome.
authors.affiliation: Departments of Pediatrics, *Public Health and Community Medicine, and #Clinical and Chemical Pathology; Faculty of Medicine, Cairo University, Cairo, Egypt. Correspondence to: Dr W Rabie, Lecturer, Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt. wl.rabie@gmail.com.
authors.forename: Walaa A
authors.initials: WA
authors.lastname: Abuelhamed
authors.forename: Nancy
authors.initials: N
authors.lastname: Zeidan
authors.forename: Walaa A
authors.initials: WA
authors.lastname: Shahin
authors.forename: Hoda I
authors.initials: HI
authors.lastname: Rizk
authors.forename: Walaa A
authors.initials: WA
authors.lastname: Rabie
mesh_terms: D003430:Cross-Sectional Studies; D004534:Egypt / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011247:Pregnancy; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*; D037701:Pulmonary Surfactant-Associated Protein B / Q000235:genetics*; D012127:Respiratory Distress Syndrome, Newborn / Q000453:epidemiology / Q000235:genetics*
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B; C445843:SFTPA2 protein, human