title: Induction of non-specific suppression in chicks by specific combination of maternal antibody and related antigen.
abstract: Specific immune suppression in newly hatched chicks induced by specific maternal antibodies has been reported. Laying hens were immunized with dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH). Purified maternal anti-DNP and non-specific immunoglobulin (Ig) Y antibodies were transferred by yolk sac inoculation to newly hatched chicks, and then, they were immunized with an optimum immunogenic dose of DNP-KLH at 1 and 4 weeks of age. Concentrations of anti-DNP antibodies in serum samples of these chicks were measured by using Enzyme-linked immunosorbent assay (ELISA). Proportions of T-cell subsets in peripheral blood of these chicks were also measured by flow cytometric analysis at 5 weeks of age (one week after the second immunization). Suppression of anti-DNP antibody response and down-regulation of CD3(+)CD4(+) cells were observed in the chicks received high dose of maternal anti-DNP antibodies and immunized with DNP-KLH. On the other hand, normal anti-DNP antibody response and normal proportion of CD3(+)CD4(+) cells were observed in the chicks received high dose of non-specific IgY antibodies and immunized with DNP-KLH. Furthermore, when chicks received high dose of maternal anti-DNP antibodies and immunized with DNP-KLH at 1 and 4 weeks of age and then with rabbit serum albumin (RSA) at 5 and 8 weeks of age, their primary anti-RSA response was also significantly suppressed. We indicate here that specific maternal antibodies can affect both B and T cell responses and induce non-specific suppression against different antigens. However, this non-specific suppression does not continue for a long time.
authors.affiliation: Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan.
authors.forename: Mohamed Fahmy
authors.initials: MF
authors.lastname: Abou Elazab
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiuchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Furusawa
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D002645:Chickens; D006433:Hemocyanins / Q000276:immunology*; D007112:Immunity, Maternally-Acquired; D007165:Immunosuppression Therapy / Q000379:methods / Q000662:veterinary; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000502:physiology*
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D012709:Serum Albumin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin