title: DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing.
abstract: In this work, a simple, general, and sensitive potentiometric platform is presented, which allows potentiometric sensing to be applied to any class of molecule irrespective of the analyte charge. DNA nanostructures are self-assembled on magnetic beads via the incorporation of an aptamer into a hybridization chain reaction. The aptamer-target binding event leads to the disassembly of the DNA nanostructures, which results in a dramatic change in the surface charge of the magnetic beads. Such a surface charge change can be sensitively detected by a polycation-sensitive membrane electrode using protamine as an indicator. With an endocrine disruptor bisphenol A as a model, the proposed potentiometric method shows a wide linear range from 0.1 to 100 nM with a low detection limit of 80 pM (3σ). The proposed sensing strategy will lay a foundation for the development of potentiometric sensors for highly sensitive and selective detection of various targets.
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation and Shandong Provincial Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, Shandong 264003, P. R. China.
authors.forename: Jiawang
authors.initials: J
authors.lastname: Ding
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation and Shandong Provincial Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, Shandong 264003, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation and Shandong Provincial Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, Shandong 264003, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation and Shandong Provincial Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, Shandong 264003, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation and Shandong Provincial Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, Shandong 264003, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis*; D004247:DNA / Q000737:chemistry*; D008280:Magnetics; D049329:Nanostructures; D011199:Potentiometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D052157:Aptamers, Nucleotide; D004247:DNA