title: [Effect of ultrafiltration-membrane extracts of Radix Rehmanniae Praeparata on proliferation and genetic stability of bone marrow-derived mesenchymal stem cells induced by cadmium chloride].
abstract: OBJECTIVE
  To study the effect of ultrafiltration-membrane extracts of Radix Rehmanniae Praeparata (UMERRP) on theproliferation and genetic stability of bone marrow-derived mesenchymal stem cells (BMSCs) induced by cadmium chloride (CdCl2).
  
  
  METHODS
  Protective effects on the proliferation, micronuclear rates, chromosome aberration rates, and apoptosis rates were observed by micronuclei test, karyotype analysis, and flow cytometry.
  
  
  RESULTS
  Compared with the CdCl2 group, UMERRP with different molecular weights at 0. 8 g/L could obviously promote the proliferation (P <0. 05). Compared with the control group, micronuclear rates, chromosome aberration rates, and apoptosis rates were obviously enhanced in the CdCl2 group (P <0. 05). Compared with the CdCl2 group, UMERRP with different molecular weights could obviously decreased CdCl2 induced micronuclear rates, chromosome aberration rates, and apoptosis rates (P <0. 05). Of them, BMSC micronuclear rates and chromosome aberration rates decreased most obvious in UMERRP groups with molecular weight below 10 000 (P <0. 05). The apoptosis rate decreased most obviously in UMERRP groups with molecular weight ranging 100 000 and 200 000 (P <0. 05).
  
  
  CONCLUSIONS
  UMERRP could reduce CdCl2 induced micronuclear rates, chromosome aberration rates, and apoptosis rates.
authors.forename: Yong-qi
authors.initials: YQ
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.lastname: Li Jing-ya
authors.forename: Rui
authors.initials: R
authors.lastname: Da
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Luo
authors.forename: Yun
authors.initials: Y
authors.lastname: Su
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Wu
authors.forename: Chun-lul
authors.initials: CL
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Nie
mesh_terms: D017209:Apoptosis; D001853:Bone Marrow; D019256:Cadmium Chloride / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D014462:Ultrafiltration
chemical_list: D004365:Drugs, Chinese Herbal; D019256:Cadmium Chloride