title: Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum.
abstract: Corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. However, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered C. glutamicum to obtain desired amounts of the target products. Therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of Escherichia coli, LdcC, were constructed using six synthetic promoters with different strengths and were examined in C. glutamicum for the production of cadaverine. Among six expression cassettes, the expression of the E. coli ldcC gene under the PH30 promoter supported the highest production of cadaverine in flask and fed-batch cultivations. A fed-batch fermentation of recombinant C. glutamicum expressing E. coli ldcC gene under the PH30 promoter resulted in the production of 40.91 g/L of cadaverine in 64 h. This report is expected to contribute toward developing engineered C. glutamicum strains to have desired features.
authors.affiliation: Center for Bio-based Chemistry, Green Chemistry & Engineering Division, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.forename: Jae Woo
authors.initials: JW
authors.lastname: Choi
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.forename: Ki Jun
authors.initials: KJ
authors.lastname: Jeong
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.forename: Il-Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Han Min
authors.initials: HM
authors.lastname: Woo
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
mesh_terms: D061249:Batch Cell Culture Techniques; D002103:Cadaverine / Q000096:biosynthesis*; D002262:Carboxy-Lyases / Q000235:genetics; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D002103:Cadaverine