title: High-sensitivity detection of ATP using a localized surface plasmon resonance (LSPR) sensor and split aptamers.
abstract: A highly sensitive localized surface plasmon resonance (LSPR) aptasensor for detection of adenosine triphosphate (ATP) has been developed. The sensor utilizes two split ATP aptamers, one (receptor fragment) being covalently attached to the surface of a gold nanorod (GNR) and the other labeled with a random DNA sequence and TAMRA dye (probe fragment). In the presence of both ATP and the probe fragment, a significant shift takes place in the wavelength of the LSPR band. This phenomenon is a consequence of the fact that the split fragments assemble into an intact folded structure in the presence of ATP, which brings about a decrease in the distance between the GNR surface and TAMRA dye and an associated LSPR wavelength. By using this sensor system, concentrations of ATP in the range of 10 pM-10 μM can be determined. In addition, by taking advantage of its denaturation properties, the LSPR aptasensor can be reused by simply subjecting it to quadruple salt-addition/2M NaCl washing steps. That the new method is applicable to biological systems was demonstrated by its use to measure ATP concentrations in E. coli and, thus to determine cell concentrations as low as 1.0×10(3) CFU.
authors.affiliation: Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712,Republic of Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712,Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Byun
authors.affiliation: Food Analysis Research Team, Industry Service Research Center, World Institute of Kimchi an Annex of Korea Food Research Institute, 86 Kimchi-ro, Nam-gu, Gwangju, Republic of Korea.
authors.forename: Won-Bo
authors.initials: WB
authors.lastname: Shim
authors.affiliation: Advanced Photonics Research Institute, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Seong U
authors.initials: SU
authors.lastname: Kim
authors.affiliation: Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712,Republic of Korea; Advanced Photonics Research Institute, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea. Electronic address: mkim@gist.ac.kr.
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes; D006046:Gold; D053768:Metal Nanoparticles; D036103:Nanotechnology; D043942:Nanotubes; D012235:Rhodamines; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012235:Rhodamines; D006046:Gold; D000255:Adenosine Triphosphate
keywords: Adenosine triphosphate; Gold nanorod; Localized surface plasmon resonance; Split aptamer