title: Effect on vascular permeability of a self-etching adhesive system containing an antimicrobial quaternary ammonium polymer QAMP into subcutaneous tissue of rats.
abstract: OBJECTIVE
  This study investigated the effect of a self-etching adhesive system containing an antimicrobial quaternary ammonium methacrylate polymer (QAMP) on the vascular permeability using Evans blue and laser-Doppler flowmetric methods.
  
  
  METHODS
  Forty rats were anesthetized and divided into groups: saline solution; Clearfil™ Protect Bond; Clearfil™ SE Bond; Clearfil™ SE Bond containing QAMP. Injections of Evans blue were administrated intravenously and the substances were injected intradermally. Immediately before injection and after 3 and 6h, was evaluated the blood flow. The animals were killed 3 and 6h after injection. The dorsal skin was dissected and experimental sites were obtained. The vascular permeability was evaluated by dye extravasation area, the dye was extracted and absorbance measured.
  
  
  RESULTS
  Concerning the Evans blue method, Clearfil™ SE Bond containing QAMP showed an extravasation area statistically similar to Clearfil™ SE Bond and lower than Clearfil™ Protect Bond. No statistical difference was verified among experimental groups for the amount of dye extracted. Clearfil™ SE Bond containing QAMP provided better laser-Doppler flowmetric parameters than Clearfil™ Protect Bond immediately and 3h after injection.
  
  
  CONCLUSIONS
  The QAMP had lower effect on the exudative phase of the inflammatory process.
authors.affiliation: Postgraduate Program in Dentistry, Department of Dentistry, State University of Ponta Grossa, Brazil. Electronic address: yasminemendes@hotmail.com.
authors.forename: Yasmine Mendes
authors.initials: YM
authors.lastname: Pupo
authors.affiliation: Laboratory of Pharmaceutical Products, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmaceutical Sciences, State University of Ponta Grossa, Brazil.
authors.forename: Paulo Vitor
authors.initials: PV
authors.lastname: Farago
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, Federal University of Parana, Brazil.
authors.forename: Jessica Mendes
authors.initials: JM
authors.lastname: Nadal
authors.affiliation: Postgraduate Program in Dentistry, Department of Dentistry, State University of Ponta Grossa, Brazil.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Kovalik
authors.affiliation: Postgraduate Program in Dentistry, Department of Dentistry, State University of Ponta Grossa, Brazil.
authors.forename: Fábio André Dos
authors.initials: FA
authors.lastname: Santos
authors.affiliation: Postgraduate Program in Dentistry, Department of Dentistry, State University of Ponta Grossa, Brazil.
authors.forename: Osnara Maria Mongruel
authors.initials: OM
authors.lastname: Gomes
authors.affiliation: Postgraduate Program in Dentistry, Department of Dentistry, State University of Ponta Grossa, Brazil.
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Gomes
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005070:Evans Blue; D005119:Extravasation of Diagnostic and Therapeutic Materials; D017078:Laser-Doppler Flowmetry; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D051381:Rats; D019279:Resin Cements / Q000737:chemistry*; D040521:Subcutaneous Tissue; D013499:Surface Properties
chemical_list: C503075:Clearfil Protect Bond; C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; D000644:Quaternary Ammonium Compounds; D019279:Resin Cements; D005070:Evans Blue
keywords: Acute-phase reaction; Evans blue; Inflammation; Laser-Doppler flowmetry; Quaternary ammonium compounds