title: RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR-101 induces apoptosis in prostate cancer.
abstract: MicroRNA-101 (miR-101) participates in carcinogenesis and tumor progression in various cancers. However, its biological functions in prostate cancer are still unclear. Here, we demonstrate that miR-101 represents a critical role in regulating cell apoptosis in prostate cancer cells. We first demonstrated that miR-101 treatment promoted apoptosis in DU145 and PC3 cells by using flow cytometric analysis and transmission electron microscopy (TEM). To verify the mechanisms, we identified a novel miR-101 target, Ral binding protein 1 (RLIP76). We found miR-101 transfection significantly suppresses RLIP76 expression, which can transactivate phosphorylation of PI3K-Akt signaling, and resulted in an amplification of Bcl2-induced apoptosis. Furthermore, we demonstrated that RLIP76 overexpression could reverse the anti-tumor effects of miR-101 in DU145 and PC3 cells by using flow cytometry assay and MTT assay. Taken together, our results revealed that the effect of miR-101 on prostate cancer cell apoptosis was due to RLIP76 regulation of the PI3K/Akt/Bcl-2 signaling pathway.
authors.affiliation: Chinese PLA General Hospital, Department of Oncology, 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Chinese PLA General Hospital, Department of Oncology, 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.affiliation: Chinese PLA General Hospital, Department of Oncology, 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Chinese PLA General Hospital, Department of Oncology, 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Chinese PLA General Hospital, Department of Oncology, 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Chinese PLA General Hospital, Department of Oncology, 28 Fuxing Road, Beijing, 100853, China. Electronic address: zhangd1117@yahoo.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D020690:GTPase-Activating Proteins / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D020690:GTPase-Activating Proteins; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; C095637:RALBP1 protein, human; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: Apoptosis; Prostate cancer; RLIP76; miR-101