title: Detection of clarithromycin-resistant Helicobacter pylori strains in a dyspeptic patient population in Sri Lanka by polymerase chain reaction-restriction fragment length polymorphism.
abstract: OBJECTIVE
  The aim of this study was to investigate the proportion of common clarithromycin-resistant mutation types present in the 23S ribosomal ribonucleic acid (rRNA) gene of H. pylori strains in Sri Lanka.
  
  
  METHODS
  The study was a cross-sectional, descriptive study where 76 dyspeptic patients who were required to undergo endoscopy examination were included. The study was carried out at a Teaching Hospital in Sri Lanka.
  
  
  METHODS
  In-house urease test and polymerase chain reaction (PCR) amplification of the glmM gene of H. pylori was performed to confirm the H. pylori infection. Analysis of point mutations in 23S rRNA gene strains were performed by PCR-restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  Of the 16 urease-positive biopsies, 94% (n=15) were positive by PCR using the glmM primer. All H. pylori strains yeilded a point mutation at A2142G site of the 23S rRNA gene, while A2143G mutation was not detected.
  
  
  CONCLUSIONS
  For the first time in Sri Lanka, we reported predominance of A2142G point mutation associated with claritromycin resistance of H. pylori in a Sri Lankan population.
authors.forename: N L
authors.initials: NL
authors.lastname: Ubhayawardana
authors.forename: M M
authors.initials: MM
authors.lastname: Weerasekera
authors.forename: D
authors.initials: D
authors.lastname: Weerasekera
authors.forename: K
authors.initials: K
authors.lastname: Samarasinghe
authors.forename: C P
authors.initials: CP
authors.lastname: Gunasekera
authors.affiliation: Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayawardenepura, Sri Lanka.
authors.forename: N
authors.initials: N
authors.lastname: Fernando
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D003430:Cross-Sectional Studies; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004415:Dyspepsia / Q000382:microbiology*; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006784:Hospitals, Teaching; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013188:Sri Lanka
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin