title: Alteration of Genetic Make-up in Karnal Bunt Pathogen (Tilletia indica) of Wheat in Presence of Host Determinants.
abstract: Alteration of genetic make-up of the isolates and monosporidial strains of Tilletia indica causing Karnal bunt (KB) disease in wheat was analyzed using DNA markers and SDS-PAGE. The generation of new variation with different growth characteristics is not a generalized feature and is not only dependant on the original genetic make up of the base isolate/monosporidial strains but also on interaction with host. Host determinant(s) plays a significant role in the generation of variability and the effect is much pronounced in monosporidial strains with narrow genetic base as compared to broad genetic base. The most plausible explanation of genetic variation in presence of host determinant(s) are the recombination of genetic material from two different mycelial/sporidia through sexual mating as well as through para-sexual means. The morphological and development dependent variability further suggests that the variation in T. indica strains predominantly derived through the genetic rearrangements.
authors.affiliation: Department of Molecular Biology and Genetic Engineering, CBSH, G.B. Pant University of Agriculture and Technology, Pantnagar.
authors.forename: Atul K
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Department of Molecular Biology and Genetic Engineering, CBSH, G.B. Pant University of Agriculture and Technology, Pantnagar.
authors.forename: J M
authors.initials: JM
authors.lastname: Seneviratne
authors.affiliation: Department of Plant Breeding and Genetics, Punjab Agriculture University, Ludhiana.
authors.forename: Ritu
authors.initials: R
authors.lastname: Bala
authors.affiliation: Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar.
authors.forename: J P
authors.initials: JP
authors.lastname: Jaiswal
authors.affiliation: Department of Molecular Biology and Genetic Engineering, CBSH, G.B. Pant University of Agriculture and Technology, Pantnagar.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
keywords: Fungal variability; Genetic divergence; ISSR; Karnal bunt; RAPD