title: Diversity of lactase persistence in African milk drinkers.
abstract: The genetic trait of lactase persistence is attributable to allelic variants in an enhancer region upstream of the lactase gene, LCT. To date, five different functional alleles, -13910*T, -13907*G, -13915*G, -14009*G and -14010*C, have been identified. The co-occurrence of several of these alleles in Ethiopian lactose digesters leads to a pattern of sequence diversity characteristic of a 'soft selective sweep'. Here we hypothesise that throughout Africa, where multiple functional alleles co-exist, the enhancer diversity will be greater in groups who are traditional milk drinkers than in non-milk drinkers, as the result of this sort of parallel selection. Samples from 23 distinct groups from 10 different countries were examined. Each group was classified 'Yes 'or 'No' for milk-drinking, and ethnicity, language spoken and geographic location were recorded. Predicted lactase persistence frequency and enhancer diversity were, as hypothesised, higher in the milk drinkers than the non-milk-drinkers, but this was almost entirely accounted for by the Afro-Asiatic language speaking peoples of east Africa. The other groups, including the 'Nilo-Saharan language speaking' milk-drinkers, show lower frequencies of LP and lower diversity, and there was a north-east to south-west decline in overall diversity. Amongst the Afro-Asiatic (Cushitic) language speaking Oromo, however, the geographic cline was not evident and the southern pastoralist Borana showed much higher LP frequency and enhancer diversity than the other groups. Together these results reflect the effects of parallel selection, the stochastic processes of the occurrence and spread of the mutations, and time depth of milk drinking tradition.
authors.affiliation: Research Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London, WC1E 6BT, UK.
authors.forename: Bryony Leigh
authors.initials: BL
authors.lastname: Jones
authors.forename: Tamiru
authors.initials: T
authors.lastname: Oljira
authors.forename: Anke
authors.initials: A
authors.lastname: Liebert
authors.forename: Pawel
authors.initials: P
authors.lastname: Zmarz
authors.forename: Nicolas
authors.initials: N
authors.lastname: Montalva
authors.forename: Ayele
authors.initials: A
authors.lastname: Tarekeyn
authors.forename: Rosemary
authors.initials: R
authors.lastname: Ekong
authors.forename: Mark G
authors.initials: MG
authors.lastname: Thomas
authors.forename: Endashaw
authors.initials: E
authors.lastname: Bekele
authors.forename: Neil
authors.initials: N
authors.lastname: Bradman
authors.forename: Dallas M
authors.initials: DM
authors.lastname: Swallow
mesh_terms: D000349:Africa; D000483:Alleles; D000818:Animals; D005260:Female; D005787:Gene Frequency; D006801:Humans; D043322:Lactase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008892:Milk; D040641:Quantitative Trait Loci
chemical_list: D043322:Lactase