title: Metabolomic analysis of serum from obese adults with hyperlipemia by UHPLC-Q-TOF MS/MS.
abstract: The prevalence of obesity has dramatically increased and poses a major threat to human health. Obesity often accompanies hyperlipemia, which is strongly related to the occurrence and development of obesity-related chronic diseases. Differences in metabolomic profiling of serum between obese (with hyperlipemia) and normal-weight men (n = 30 in each group) were investigated using ultrahigh-pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF MS/MS) and partial least-squares-discriminant analysis (PLS-DA). Obese men showed higher levels of weight, body mass index, fat mass, systolic blood pressure, fasting plasma glucose, triglyeride, total cholesterol, insulin, HOMA-IR and high-sensitivity CRP. Obese and normal-weight groups were clearly discriminated from each other on a PLS-DA score plot and nine major metabolites contributing to the discrimination were assigned, including increased 2-octenoylcarnitine, eicosadienoic acid, 12-hydroperoxyeicosatetraenoic acid, 4-hydroxyestrone sulfate, lysoPE[18:1(11Z)/0:0], thromboxane B2 and pyridinoline and decreased vitamin D3 glucosiduronate and 9,10-DHOME. These metabolites were associated with lipid metabolism and obesity-related diseases, and reflected the metabolic differences between normal and obese men, which may be important for future clinical diagnosis, treatment and assessment of the therapeutic effect on obesity-related chronic disease.
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Desheng
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Enyou
authors.initials: E
authors.lastname: Li
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006894:Hydroxyestrones / Q000097:blood; D006949:Hyperlipidemias / Q000097:blood*; D008297:Male; D055432:Metabolomics; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D044967:Serum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D006894:Hydroxyestrones; C026418:4-hydroxyestrone
keywords: UHPLC-Q-TOF MS/MS; metabolism; metabonomics; obesity