title: Generation of transgenic rice with reduced content of major and novel high molecular weight allergens.
abstract: BACKGROUND
  Rice seed proteins contain antigens that provoke allergic responses in some individuals with food allergy, particularly in those with cereal allergy, and these antigens can elicit clinical symptoms such as eczema and dermatitis. We previously generated transgenic rice with reduced accumulation of the three major allergens, which dramatically reduced the level of IgE binding from patients' sera. However, the transgenic rice still possesses allergenic reactivity. Recently, two globulin-like proteins were identified as candidates of novel high molecular weight (HMW) IgE-binding proteins that cause rice allergy.
  
  
  RESULTS
  We identified a glucosidase family encoded by four genes as novel HMW rice allergens based on IgE antibody reactivity from individuals with allergy to rice. To further reduce allergenicity, we generated transgenic rice with reduced accumulation of these HMW allergens. We crossed the rice with reduced HMW allergens and with reduced major allergens, and all major and HMW allergens were substantially reduced in the progeny of the crossed rice. Allergen suppression did not significantly alter accumulation patterns of seed storage proteins and protein folding enzymes. The sera of a portion of patients showed low IgE-binding to the crossed line, suggesting that the crossed line is effective for a portion of patients who are allergic to proteins other than major allergens.
  
  
  CONCLUSIONS
  The transgenic rice with reduced levels of all major and HMW allergens is thought to be an option for a portion of allergy patients with hypersensitive responses to various kinds of rice allergens.
authors.affiliation: Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 3-1-3, Tsukuba, Ibaraki, 305-8604, Japan, ogogogo@affrc.go.jp.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogo
authors.forename: Yuhya
authors.initials: Y
authors.lastname: Wakasa
authors.forename: Kana
authors.initials: K
authors.lastname: Hirano
authors.forename: Atsuo
authors.initials: A
authors.lastname: Urisu
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa