title: Structural, Dynamical, and Energetical Consequences of Rett Syndrome Mutation R133C in MeCP2.
abstract: Rett Syndrome (RTT) is a progressive neurodevelopmental disease affecting females. RTT is caused by mutations in the MECP2 gene and various amino acid substitutions have been identified clinically in different domains of the multifunctional MeCP2 protein encoded by this gene. The R133C variant in the methylated-CpG-binding domain (MBD) of MeCP2 is the second most common disease-causing mutation in the MBD. Comparative molecular dynamics simulations of R133C mutant and wild-type MBD have been performed to understand the impact of the mutation on structure, dynamics, and interactions of the protein and subsequently understand the disease mechanism. Two salt bridges within the protein and two critical hydrogen bonds between the protein and DNA are lost upon the R133C mutation. The mutation was found to weaken the interaction with DNA and also cause loss of helicity within the 141-144 region. The structural, dynamical, and energetical consequences of R133C mutation were investigated in detail at the atomic resolution. Several important implications of this have been shown regarding protein stability and hydration dynamics as well as its interaction with DNA. The results are in agreement with previous experimental studies and further provide atomic level understanding of the molecular origin of RTT associated with R133C variant.
authors.affiliation: Computational Biophysics and Bioinformatics, Department of Physics, Clemson University, Clemson, SC 29634, USA.
authors.forename: Tugba G
authors.initials: TG
authors.lastname: Kucukkal
authors.affiliation: Computational Biophysics and Bioinformatics, Department of Physics, Clemson University, Clemson, SC 29634, USA.
authors.forename: Emil
authors.initials: E
authors.lastname: Alexov
mesh_terms: D019943:Amino Acid Substitution; D019295:Computational Biology; D018360:Crystallography, X-Ray; D005260:Female; D006801:Humans; D006860:Hydrogen Bonding; D051783:Methyl-CpG-Binding Protein 2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D009154:Mutation; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D055550:Protein Stability; D015518:Rett Syndrome / Q000235:genetics* / Q000378:metabolism*; D055672:Static Electricity
chemical_list: C497392:MECP2 protein, human; D051783:Methyl-CpG-Binding Protein 2