title: The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.
abstract: Peptide ligands play crucial roles in the life cycle of plants by modulating the innate immunity against pathogens and regulating growth and developmental processes. One well-studied example is INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), which controls floral organ abscission and lateral root emergence in Arabidopsis thaliana. IDA belongs to a family of five additional IDA-LIKE (IDL) members that have all been suggested to be involved in regulation of Arabidopsis development. Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses. Furthermore, we provide data that the recently identified PAMP-INDUCED SECRETED PEPTIDE (PIP) and PIP-LIKE (PIPL) peptides, which show similarity to the IDL and C-TERMINALLY ENCODED PEPTIDE (CEP) peptides, are not only involved in innate immune response in Arabidopsis but are also induced by abiotic stress. Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines. Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Ane Kjersti
authors.initials: AK
authors.lastname: Vie
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Javad
authors.initials: J
authors.lastname: Najafi
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Per
authors.initials: P
authors.lastname: Winge
authors.affiliation: Department of Biosciences, University of Oslo, N-0316 Oslo, Norway.
authors.forename: Melinka A
authors.initials: MA
authors.lastname: Butenko
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway Department of Biosciences, University of Oslo, N-0316 Oslo, Norway.
authors.forename: Karina S
authors.initials: KS
authors.lastname: Hornslien
authors.affiliation: Department of Biosciences, University of Oslo, N-0316 Oslo, Norway.
authors.forename: Robert
authors.initials: R
authors.lastname: Kumpf
authors.affiliation: Department of Biosciences, University of Oslo, N-0316 Oslo, Norway.
authors.forename: Reidunn B
authors.initials: RB
authors.lastname: Aalen
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Atle M
authors.identifier: http://orcid.org/0000-0003-0544-7437
authors.initials: AM
authors.lastname: Bones
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway tore.brembu@ntnu.no.
authors.forename: Tore
authors.identifier: http://orcid.org/0000-0001-5622-8037
authors.initials: T
authors.lastname: Brembu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D010455:Peptides
keywords: Arabidopsis; INFLORESCENCE DEFICIENT IN ABSCISSION; evolution; gene expression; peptide ligand.