title: Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities.
abstract: We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and fluorescence-activated cell sorting.
authors.affiliation: Department of Chemistry, University of Chicago, 929 E57th Street, Chicago, IL, 60637, USA.
authors.forename: Keya
authors.initials: K
authors.lastname: Zhang
authors.forename: Karan
authors.initials: K
authors.lastname: Bhuripanyo
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D002384:Catalysis; D002469:Cell Separation; D061505:Cell Surface Display Techniques; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D019151:Peptide Library; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D019151:Peptide Library