title: 5'-UTR of malS increases the invasive capacity of Salmonella enterica serovar Typhi by influencing the expression of bax.
abstract: OBJECTIVE
  An RNA-seq analysis recently identified a 236-nucleotide transcript upstream from malS in Salmonella enterica serovar Typhi. Here, we investigated its molecular characteristics and function.
  
  
  METHODS
  RACE and northern blotting were used to determine the molecular characteristics of the sequence, and mutagenesis, microarray, immunoblotting and an invasion assay were used to investigate the functions of the transcript.
  
  
  RESULTS
  The transcript was identified as the malS 5'-untranslated region (UTR), which could influence the expression of the flagellar and SPI-1 genes and the invasion of HeLa cells by S. Typhi. Deletion of bax increased the expression of the invasion genes and the invasive capacity of S. Typhi, whereas the expression of the malS 5'-UTR reduced the expression of bax.
  
  
  CONCLUSIONS
  The malS 5'-UTR reduces the expression of bax and increases the invasive capacity of S. Typhi.
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.|Department of Biochemistry, Chengde Medical College, Chengde, Hebei 067000, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Gong
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Shungao
authors.initials: S
authors.lastname: Xu
authors.affiliation: Clinical Laboratory, The Affiliated Huai'an Hospital of Xuzhou Medical College, Huai'an, 223002, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Isaac
authors.initials: I
authors.lastname: Dadzie
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Zhexin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ni
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
mesh_terms: D020121:5' Untranslated Regions; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004705:Endocytosis; D004847:Epithelial Cells / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D012485:Salmonella typhi / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins
keywords: 5′-UTR; S. Typhi; bax; invasiveness; malS