title: Expression of 3-phosphoinositide-dependent protein kinase 1 in colorectal cancer as a potential therapeutic target.
abstract: 3-Phosphoinositide-dependent protein kinase 1 (PDK1) is centrally involved in cancer progression, including proliferation, apoptosis and invasion. However, its expression pattern and possible cellular functions in human colorectal cancer remain unclear. In the present study, we show that PDK1 expression is up-regulated at both mRNA and protein levels in colorectal cancer clinical specimens and cell lines. Transient knockdown of PDK1 suppresses cellular growth, induces cellular apoptosis and causes abnormal cell cycle distribution. Meanwhile, decreased PDK1 level is closely associated with reduced Akt/cyclin D1 activity. Activating AKT activity and reintroducing cyclin D1 expression significantly compromised the oncogenic activity induced by PDK1. Together, our findings elucidate a key role for PDK1 in colorectal cellular functions trigged by the Akt/cyclin D1 pathway, thus providing a novel insight of PDK1 in colorectal carcinogenesis.
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, 1017#, North Dongmen Road, Shenzhen, 518000, Guangdong Province, People's Republic of China.
authors.forename: Zhenglei
authors.initials: Z
authors.lastname: Xu
authors.forename: Bihong
authors.initials: B
authors.lastname: Liao
authors.forename: Ru
authors.initials: R
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Ruiyue
authors.initials: R
authors.lastname: Shi
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D045325:HCT116 Cells; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C000631429:PDK1 protein, human; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D012333:RNA, Messenger; D019938:Cyclin D1; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt