title: Biocontrol Activity of Bacillus subtilis Isolated from Agaricus bisporus Mushroom Compost Against Pathogenic Fungi.
abstract: Bacillus subtilis strain B154, isolated from Agaricus bisporus mushroom compost infected by red bread mold, exhibited antagonistic activities against Neurospora sitophila. Antifungal activity against phytopathogenic fungi was also observed. The maximum antifungal activity was reached during the stationary phase. This antifungal activity was stable over a wide pH and temperature range and was not affected by proteases. Assay of antifungal activity in vitro indicated that a purified antifungal substance could strongly inhibit mycelia growth and spore germination of N. sitophila. In addition, treatment with strain B154 in A. bisporus mushroom compost infected with N. sitophila significantly increased the yield of bisporus mushrooms. Ultraviolet scan spectroscopy, tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-associated laser desorption ionization time-of-flight mass spectrometry, and electrospray ionization tandem mass spectrometry analyses revealed a molecular weight consistent with 1498.7633 Da. The antifungal compound might belong to a new type of lipopeptide fengycin.
authors.affiliation: †College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.|§Bioorganic and Natural Products Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, Massachusetts 02478, United States.
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.affiliation: ‡School of Agricultural Economics and Rural Development, Renmin University of China, 59 Zhong Guancun Street, Haidian District, Beijing 100872, China.|§Bioorganic and Natural Products Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, Massachusetts 02478, United States.
authors.forename: Jiping
authors.initials: J
authors.lastname: Sheng
authors.affiliation: †College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: †College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: §Bioorganic and Natural Products Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, Massachusetts 02478, United States.
authors.forename: David Yue Wei
authors.initials: DY
authors.lastname: Lee
authors.affiliation: †College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: †College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
authors.forename: Mingshuang
authors.initials: M
authors.lastname: Xu
authors.affiliation: †College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
mesh_terms: D000364:Agaricus / Q000254:growth & development*; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013058:Mass Spectrometry; D008970:Molecular Weight; D009491:Neurospora / Q000187:drug effects* / Q000254:growth & development; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014675:Vegetables / Q000254:growth & development / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D012987:Soil
keywords: Agaricus bisporus; Bacillus subtilis; antifungal activity; biological control; fengycin