title: Studies on Unprocessed and Acid-Treated Arabinogalactan from Larch as an Inhibitor of Glycan Binding of a Plant Toxin and Biomedically Relevant Human Lectins.
abstract: The increasing evidence for the physiological significance of glycan-protein (lectin) interactions prompts considerations for respective bioactivity of plant polysaccharides. Arabinogalactan from larch, a polysaccharide with a β1,3-linked galactose core and branches at the 6'-hydroxyl, was thus tested, together with two processed forms treated either with oxalic or trifluoroacetic acid. Hydrolysis by acid reduced the arabinose contents without backbone degradation. The three preparations were tested as an inhibitor of lectin binding in solid-phase and cell-based assays, using the toxin from Viscum album and a panel of seven human lectins (six galectins and a C-type lectin). Increasing potency correlating with the molecular contents of galactose was seen for the plant toxin. In general, relatively weak or no inhibitory capacity was detected for the three preparations, when binding of the human galectins and avian orthologues used as controls was measured. Acid-treated polysaccharides also weakly interfered with binding of the galactose-specific C-type lectin of human macrophages. Larch arabinogalactan, tested as a model, will thus most likely not impair (ga)lectin functionality physiologically.
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, München, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.affiliation: Institute of Pharmacy, Department of Pharmaceutical Biology, University of Kiel, Kiel, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Classen
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, München, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
mesh_terms: D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005690:Galactose / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D028221:Larix / Q000737:chemistry*; D037102:Lectins / Q000037:antagonists & inhibitors / Q000378:metabolism; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014118:Toxins, Biological / Q000037:antagonists & inhibitors* / Q000378:metabolism; D028182:Viscum album / Q000737:chemistry*
chemical_list: D005685:Galactans; D037102:Lectins; D011134:Polysaccharides; D014118:Toxins, Biological; C005653:arabinogalactan; D005690:Galactose