title: Proposal of a Twin Arginine Translocator System-Mediated Constraint against Loss of ATP Synthase Genes from Nonphotosynthetic Plastid Genomes. [Corrected].
abstract: Organisms with nonphotosynthetic plastids often retain genomes; their gene contents provide clues as to the functions of these organelles. Yet the functional roles of some retained genes-such as those coding for ATP synthase-remain mysterious. In this study, we report the complete plastid genome and transcriptome data of a nonphotosynthetic diatom and propose that its ATP synthase genes may function in ATP hydrolysis to maintain a proton gradient between thylakoids and stroma, required by the twin arginine translocator (Tat) system for translocation of particular proteins into thylakoids. Given the correlated retention of ATP synthase genes and genes for the Tat system in distantly related nonphotosynthetic plastids, we suggest that this Tat-related role for ATP synthase was a key constraint during parallel loss of photosynthesis in multiple independent lineages of algae/plants.
authors.affiliation: Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan kamikawa.ryoma.7v@kyoto-u.ac.jp.
authors.forename: Ryoma
authors.initials: R
authors.lastname: Kamikawa
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Tanifuji
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Sohta A
authors.initials: SA
authors.lastname: Ishikawa
authors.affiliation: Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan.
authors.forename: Yusei
authors.initials: Y
authors.lastname: Matsuno
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada.
authors.forename: Naoko T
authors.initials: NT
authors.lastname: Onodera
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Ishida
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan Center for Computational Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Miyashita
authors.affiliation: Department of Biology, Tokyo Gakugei University, Tokyo, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Mayama
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan Center for Computational Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Inagaki
mesh_terms: D025222:Chloroplast Proton-Translocating ATPases / Q000378:metabolism*; D017377:Diatoms / Q000235:genetics*; D054627:Genome, Plastid; D008954:Models, Biological; D010788:Photosynthesis; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D000069294:Twin-Arginine-Translocation System / Q000378:metabolism*
chemical_list: D000069294:Twin-Arginine-Translocation System; D025222:Chloroplast Proton-Translocating ATPases
keywords: ATP synthase complex; apochlorotic diatoms; genome reduction; nonphotosynthetic plastid genome; twin arginine translocator