title: Effect of Qnr on Plasmid Gyrase Toxins CcdB and ParE.
abstract: Plasmid toxins CcdB and ParE are part of addiction systems promoting plasmid maintenance. Both target host DNA gyrase, as do quinolones and plasmid-determined Qnr proteins that protect gyrase from quinolone inhibition. We cloned qnrB4, qnrS1, ccdB, parE, and the antitoxin-encoding genes ccdA and parD on compatible plasmids and tested them in combination. CcdB and ParE had no specific effect on quinolone susceptibility or Qnr protection, and Qnr did not act as a CcdB or ParE antitoxin.
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA Inje University College of Medicine, Busan, South Korea.
authors.forename: Yee Gyung
authors.initials: YG
authors.lastname: Kwak
authors.affiliation: Lahey Hospital and Medical Center, Burlington, Massachusetts, USA george.a.jacoby@lahey.org.
authors.forename: George A
authors.initials: GA
authors.lastname: Jacoby
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Hooper
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000992:Antitoxins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D002939:Ciprofloxacin / Q000494:pharmacology*; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D059005:Topoisomerase II Inhibitors / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000992:Antitoxins; D001426:Bacterial Proteins; C068356:CcdB protein, Plasmid F; D003603:Cytotoxins; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; D059005:Topoisomerase II Inhibitors; D002939:Ciprofloxacin; D011938:Rec A Recombinases; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase