title: Multistrip Western blotting: a tool for comparative quantitative analysis of multiple proteins.
abstract: The qualitative and quantitative measurements of protein abundance and modification states are essential in understanding their functions in diverse cellular processes. Typical Western blotting, though sensitive, is prone to produce substantial errors and is not readily adapted to high-throughput technologies. Multistrip Western blotting is a modified immunoblotting procedure based on simultaneous electrophoretic transfer of proteins from multiple strips of polyacrylamide gels to a single membrane sheet. In comparison with the conventional technique, Multistrip Western blotting increases data output per single blotting cycle up to tenfold; allows concurrent measurement of up to nine different total and/or posttranslationally modified protein expression obtained from the same loading of the sample; and substantially improves the data accuracy by reducing immunoblotting-derived signal errors. This approach enables statistically reliable comparison of different or repeated sets of data and therefore is advantageous to apply in biomedical diagnostics, systems biology, and cell signaling research.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA.
authors.forename: Edita
authors.initials: E
authors.lastname: Aksamitiene
authors.forename: Jan B
authors.initials: JB
authors.lastname: Hoek
authors.forename: Anatoly
authors.initials: A
authors.lastname: Kiyatkin
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008163:Luminescent Measurements; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate