title: Glucose- and mannose-induced stomatal closure is mediated by ROS production, Ca(2+) and water channel in Vicia faba.
abstract: Sugars act as vital signaling molecules that regulate plant growth, development and stress responses. However, the effects of sugars on stomatal movement have been unclear. In our study, we explored the effects of monosaccharides such as glucose and mannose on stomatal aperture. Here, we demonstrate that glucose and mannose trigger stomatal closure in a dose- and time-dependent manner in epidermal peels of broad bean (Vicia faba). Pharmacological studies revealed that glucose- and mannose-induced stomatal closure was almost completely inhibited by two reactive oxygen species (ROS) scavengers, catalase (CAT) and reduced glutathione (GSH), was significantly abolished by an NADPH oxidase inhibitor, diphenylene iodonium chloride (DPI), whereas they were hardly affected by a peroxidase inhibitor, salicylhydroxamic acid (SHAM). Furthermore, glucose- and mannose-induced stomatal closure was strongly inhibited by a Ca(2+) channel blocker, LaCl3 , a Ca(2+) chelator, ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA) and two water channel blockers, HgCl2 and dimethyl sulfoxide (DMSO); whereas the inhibitory effects of the water channel blockers were essentially abolished by the reversing agent β-mercaptoethanol (β-ME). These results suggest that ROS production mainly via NADPH oxidases, Ca(2+) and water channels are involved in glucose- and mannose-induced stomatal closure.
authors.affiliation: Institute of Ecology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Ecology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: ShanShan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Ecology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Institute of Ecology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Pan
authors.affiliation: Institute of Ecology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: GenXuan
authors.initials: G
authors.lastname: Wang
mesh_terms: D020346:Aquaporins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology*; D049990:Membrane Transport Modulators / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D009861:Onium Compounds / Q000494:pharmacology; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012457:Salicylamides / Q000494:pharmacology; D031307:Vicia faba / Q000187:drug effects / Q000502:physiology*
chemical_list: D020346:Aquaporins; D049990:Membrane Transport Modulators; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D012457:Salicylamides; C028521:lanthanum chloride; D004533:Egtazic Acid; D008627:Mercuric Chloride; C007517:diphenyleneiodonium; D007811:Lanthanum; C005703:salicylhydroxamic acid; D002374:Catalase; D005978:Glutathione; D005947:Glucose; D008358:Mannose; D002118:Calcium; D004121:Dimethyl Sulfoxide