title: Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea.
abstract: Stria vascularis of the mammalian cochlea transports K(+) to establish the electrochemical property in the endolymph crucial for hearing. This epithelial tissue also transports various small molecules. To clarify the profile of proteins participating in the transport system in the stria vascularis, membrane components purified from the stria of adult rats were analysed by liquid chromatography tandem mass spectrometry. Of the 3236 proteins detected in the analysis, 1807 were membrane proteins. Ingenuity Knowledge Base and literature data identified 513 proteins as being expressed on the 'plasma membrane', these included 25 ion channels and 79 transporters. Sixteen of the former and 62 of the latter had not yet been identified in the stria. Unexpectedly, many Cl(-) and Ca(2+) transport systems were found, suggesting that the dynamics of these ions play multiple roles. Several transporters for organic substances were also detected. Network analysis demonstrated that a few kinases, including protein kinase A, and Ca(2+) were key regulators for the strial transports. In the library of channels and transporters, 19 new candidates for uncloned deafness-related genes were identified. These resources provide a platform for understanding the molecular mechanisms underlying the epithelial transport essential for cochlear function and the pathophysiological processes involved in hearing disorders.
authors.affiliation: Department of Molecular Physiology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata, 951-8510, Japan.|Center for Transdisciplinary Research, Niigata University, Niigata, Japan.|Department of Otorhinolaryngology - Head and Neck Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Uetsuka
authors.affiliation: Department of Molecular Physiology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata, 951-8510, Japan.|Center for Transdisciplinary Research, Niigata University, Niigata, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Ogata
authors.affiliation: Division of Bio-system Pharmacology, Department of Pharmacology, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Shushi
authors.initials: S
authors.lastname: Nagamori
authors.affiliation: Division of Bio-system Pharmacology, Department of Pharmacology, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Isozumi
authors.affiliation: Department of Molecular Physiology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata, 951-8510, Japan.|Center for Transdisciplinary Research, Niigata University, Niigata, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Nin
authors.affiliation: Department of Molecular Physiology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata, 951-8510, Japan.|Center for Transdisciplinary Research, Niigata University, Niigata, Japan.|Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Komune
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan.|Department of Otorhinolaryngology - Head and Neck Surgery, Nara Medical University, Nara, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kitahara
authors.affiliation: Mammalian Genetics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kikkawa
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Inohara
authors.affiliation: Division of Bio-system Pharmacology, Department of Pharmacology, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Kanai
authors.affiliation: Department of Molecular Physiology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata, 951-8510, Japan.|Center for Transdisciplinary Research, Niigata University, Niigata, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hibino
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D003638:Deafness / Q000235:genetics / Q000503:physiopathology*; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D040901:Proteomics; D051381:Rats; D013316:Stria Vascularis / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins
keywords: ion channel; mass spectrometry; proteomics; rat; transporter