title: Simultaneous extraction, identification and quantification of phenolic compounds in Eclipta prostrata using microwave-assisted extraction combined with HPLC-DAD-ESI-MS/MS.
abstract: A simple and rapid method was developed using microwave-assisted extraction (MAE) combined with HPLC-DAD-ESI-MS/MS for the simultaneous extraction, identification, and quantification of phenolic compounds in Eclipta prostrata, a common herb and vegetable in China. The optimized parameters of MAE were: employing 50% ethanol as solvent, microwave power 400 W, temperature 70 °C, ratio of liquid/solid 30 mL/g and extraction time 2 min. Compared to conventional extraction methods, the optimized MAE can avoid the degradation of the phenolic compounds and simultaneously obtained the highest yields of all components faster with less consumption of solvent and energy. Six phenolic acids, six flavonoid glycosides and one coumarin were firstly identified. The phenolic compounds were quantified by HPLC-DAD with good linearity, precision, and accuracy. The extract obtained by MAE showed significant antioxidant activity. The proposed method provides a valuable and green analytical methodology for the investigation of phenolic components in natural plants.
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address: sdauwangjh@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Taishan Medical University, Taian, Shandong 271000, China.
authors.forename: Jifu
authors.initials: J
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Xueke
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
mesh_terms: D000975:Antioxidants; D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036601:Eclipta / Q000737:chemistry*; D005419:Flavonoids; D062385:Hydroxybenzoates / Q000737:chemistry*; D008872:Microwaves; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D001688:Biological Products; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid
keywords: Antioxidant activity; Eclipta prostrata L.; Green analytical chemistry; HPLC–DAD–ESI–MS/MS; Microwave assisted extraction; Phenolic compounds