title: Association of rs12255372 (TCF7L2) and D76N (PDX-1) Polymorphisms with Type 2 Diabetes in a Population Living in Northeast Iran.
abstract: The aim of this study was to investigate whether the rs12255372 (TCF7L2) and D76N (PDX-1) polymorphisms are associated with type 2 diabetes mellitus (T2DM) in Mashhad, northeast Iran. A hundred twenty seven patients with T2DM and 71 non-diabetic controls in Mashhad were genotyped by PCR-RFLP and ARMS-PCR methods. Single nucleotide polymorphisms (SNPs) were confirmed by sequencing in some samples and allelic and genotypic frequencies were then analyzed in each group. The T-allele of the SNP rs12255372 of TCF7L2 (OR = 2.70, 95% CI = 1.12-6.49, P = 0.027) and the A-allele of PDX-1 D76N (OR = 3.93, 95% CI = 1.60-7.68, P = 0.002) were significantly associated with an increased risk of T2DM. The rs12255372 SNP of TCF7L2 and D76N of PDX-1 genes may confer susceptibility to T2DM in the population living in Mashhad.
authors.affiliation: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Roya
authors.initials: R
authors.lastname: Rafati
authors.affiliation: 1)Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. 2)Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.razieh@um.ac.ir.
authors.forename: Razieh
authors.initials: R
authors.lastname: Jalal
authors.affiliation: 1)Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. 2)Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Asoodeh
authors.affiliation: 2)Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.3)Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Maryam M
authors.initials: MM
authors.lastname: Matin
mesh_terms: D000483:Alleles; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D015534:Trans-Activators / Q000235:genetics*; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; C544894:TCF7L2 protein, human; D015534:Trans-Activators; D058118:Transcription Factor 7-Like 2 Protein; C084010:pancreatic and duodenal homeobox 1 protein