title: Crystallization and preliminary crystallographic analysis of the major acid phosphatase from Legionella pneumophila.
abstract: The major acid phosphatase from Legionella pneumophila (LpMAP) belongs to the histidine acid phosphatase superfamily. It contains the characteristic histidine acid phosphatase (HAP) sequence motif RHGXRXP responsible for the hydrolysis of a phosphoryl group from phosphate monoesters under acidic conditions. Here, the crystallization and preliminary X-ray analysis of crystals of LpMAP in the apo form and in complex with L-(+)-tartrate are described. By using the hanging-drop vapour-diffusion method, apo LpMAP and LpMAP-tartrate were crystallized in space group P2(1), with unit-cell parameters a = 91.50, b = 56.48, c = 146.35 Å, β = 110.01°, and in space group P1, with unit-cell parameters a = 55.51, b = 73.51, c = 98.78 Å, α = 78.82, β = 77.65, γ = 67.73°, respectively. Diffraction data were collected at 100 K and the phases were determined using the molecular-replacement method.
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei 230601, People's Republic of China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Ge
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry* / Q000235:genetics; D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016952:Legionella pneumophila / Q000737:chemistry* / Q000201:enzymology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000135:Acid Phosphatase
keywords: Legionella pneumophila major acid phosphatase; acid phosphatase; histidine acid phosphatase