title: Up-regulation of iNOS by hypoxic postconditioning inhibits H9c2 cardiomyocyte apoptosis induced by hypoxia/re-oxygenation.
abstract: Apoptosis is a crucial mode of cell death induced by ischemia and reperfusion, and ischemic postconditioning (PostC) has been reported to inhibit cell apoptosis. Inducible nitric oxide synthase (iNOS) has been confirmed to play an important role in triggering and mediating the late cardio-protection against ischemia/hypoxia. In this study, we found that hypoxic PostC remarkably up-regulated the expression of iNOS and decreased cardiomyocyte apoptosis. Pre-treatment with 1400w (a highly selective inhibitor of iNOS) or iNOS siRNA weakened the anti-apoptotic effect of hypoxic PostC. These findings suggested that iNOS may be one of the key molecular mechanisms responsible for the inhibition of apoptosis by hypoxic PostC.
authors.affiliation: Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China zhaohx5@126.com.
authors.forename: Huanxin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Wanfang
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Guoyi
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Center for Cardiovascular Sciences, Albany Medical Center, Albany, NY 12208, USA.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D020868:Gene Silencing; D057775:Ischemic Postconditioning; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015854:Up-Regulation
chemical_list: D052247:Nitric Oxide Synthase Type II
keywords: apoptosis; cardiomyocyte; hypoxic postconditioning; inducible nitric oxide synthase