title: Development of a programmable piggyback syringe pump and four-times-a-day injection regimen for superovulation in non-lactating Holstein cows.
abstract: The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3- < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively).
authors.affiliation: United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan.
authors.forename: Abdul Razaq
authors.initials: AR
authors.lastname: Irshad
authors.forename: Taihei
authors.initials: T
authors.lastname: Sasaki
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kubo
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Odashima
authors.forename: Keiji
authors.initials: K
authors.lastname: Katano
authors.forename: Takeshi
authors.initials: T
authors.lastname: Osawa
authors.forename: Toru
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Izaike
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D001415:Back; D002417:Cattle; D003338:Corpus Luteum / Q000166:cytology / Q000000981:diagnostic imaging / Q000187:drug effects*; D003612:Dairying; D004334:Drug Administration Schedule / Q000662:veterinary; D004973:Estrus Synchronization / Q000187:drug effects; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage*; D007260:Infusion Pumps / Q000662:veterinary*; D007273:Injections, Intramuscular; D007700:Kinetics; D009929:Organ Size; D006080:Ovarian Follicle / Q000166:cytology / Q000000981:diagnostic imaging / Q000187:drug effects*; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D013480:Superovulation / Q000097:blood / Q000187:drug effects*; D034421:Sus scrofa; D014463:Ultrasonography
chemical_list: D005300:Fertility Agents, Female; D011374:Progesterone; D005640:Follicle Stimulating Hormone