title: Identification of Actinobacillus pleuropneumoniae Genes Preferentially Expressed During Infection Using In Vivo-Induced Antigen Technology (IVIAT).
abstract: Porcine pleuropneumonia is an infectious disease caused by Actinobacillus pleuropneumoniae. The identification of A. pleuropneumoniae genes, specially expressed in vivo, is a useful tool to reveal the mechanism of infection. IVIAT was used in this work to identify antigens expressed in vivo during A. pleuropneumoniae infection, using sera from individuals with chronic porcine pleuropneumonia. Sequencing of DNA inserts from positive clones showed 11 open reading frames with high homology to A. pleuropneumoniae genes. Based on sequence analysis, proteins encoded by these genes were involved in metabolism, replication, transcription regulation, and signal transduction. Moreover, three function-unknown proteins were also indentified in this work. Expression analysis using quantitative real-time PCR showed that most of the genes tested were up-regulated in vivo relative to their expression levels in vitro. IVI (in vivoinduced) genes that were amplified by PCR in different A. pleuropneumoniae strains showed that these genes could be detected in almost all of the strains. It is demonstrated that the identified IVI antigen may have important roles in the infection of A. pleuropneumoniae.
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Yangyi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Qigui
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University, Chengdu 611130, P.R. China.
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D005798:Genes, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D015533:Transcriptional Activation
chemical_list: D000942:Antigens, Bacterial
keywords: Actinobacillus pleuropneumoniae; Differential expression; In vivo-induced genes