title: Effects of minocycline-HCl paste root conditioning on periodontal surgery: in vitro and in vivo studies.
abstract: OBJECTIVE
  This study was aimed to investigate effects of 2% minocycline-HCl paste root conditioning on periodontal surgery.
  
  
  METHODS
  In vitro, cementum slices affected by periodontitis were randomly conditioned with 2% minocycline-HCl paste, 2% minocycline-HCl liquid, and 0.9% saline. NIH3T3 cells were cultured and attached to the each slide, and the viability and proliferation of cells were observed; In vivo, 21 deep periodontal pockets were treated by periodontal surgery, and the exposed root surfaces were randomly conditioned with 2% minocycline-HCl paste or 0.9% saline. The periodontal parameters were measured at baseline, after 3 and 6 months of periodontal surgery.
  
  
  RESULTS
  In vitro, NIH3T3 cell showed better viability and proliferation at 3, 5, and 7 day in groups conditioned with minocycline-HCl than the group conditioned with 0.9% saline (at 3 day (P < 0.05); at 7 day (P < 0.01)) Minimal differences were found between minocycline-HCl paste and liquid groups; In vivo, 3 months after periodontal surgery, the greater CAL reduction was found in the minocycline-HCl treated group than in the control group (P < 0.05). The similar results were found for both CAL and PD (P < 0.05; P < 0.05) between two groups at 6 months after surgery. PI and SBI variations showed no statistical differences between two groups after periodontal surgery.
  
  
  CONCLUSIONS
  Our results suggested that root conditioning with minocycline-HCl paste during periodontal surgery improve the periodontal healing, which may be associated with the promotion of the periodontal cell attachment and growth onto the root surfaces.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China ; Department of Periodontology, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China ; Department of Periodontology, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China ; Department of Periodontology, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China.
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Li
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China ; Department of Periodontology, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China.
authors.forename: Shuhuan
authors.initials: S
authors.lastname: Shang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China ; Department of Periodontology, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China.
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
keywords: Root conditioning; minocycline-HCl; periodontal surgery