title: Fate of β-hexachlorocyclohexane in the mixed microbial cultures (MMCs) three-stage polyhydroxyalkanoates (PHA) production process from cheese whey.
abstract: This work aimed to study the fate and effect of β-hexachlorocyclohexane (β-HCH) during several steps of PHA production and purification, by using an artificially contaminated cheese whey (CW) as the feedstock. Most of β-HCH (around 90%) was adsorbed on CW solids and it was removed after the acidogenic fermentation step, when residual CW solids are separated along with anaerobic biomass from the liquid-phase. Purification steps also contributed strongly to the removal of residual β-HCH; overall, the PHA production process removed about 99.9% of initial β-HCH content. Moreover, it has been shown that β-HCH has neither detrimental effect on acidogenic fermentation nor on PHA accumulation, that were performed by using unacclimated mixed microbial cultures.
authors.affiliation: Dept. of Chemistry, "Sapienza" University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Electronic address: francesco.valentino@uniroma1.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Valentino
authors.affiliation: INAIL, Settore Ricerca, Certificazione e Verifica, Monte Porzio Catone, Rome, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Riccardi
authors.affiliation: Dept. of Chemistry, "Sapienza" University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Campanari
authors.affiliation: INAIL, Settore Ricerca, Certificazione e Verifica, Monte Porzio Catone, Rome, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Pomata
authors.affiliation: Dept. of Chemistry, "Sapienza" University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Majone
mesh_terms: D000143:Acids / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002611:Cheese / Q000382:microbiology*; D005285:Fermentation / Q000502:physiology; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D000067796:Whey / Q000378:metabolism*; D000067816:Whey Proteins / Q000378:metabolism
chemical_list: D000143:Acids; D054813:Polyhydroxyalkanoates; D000067816:Whey Proteins; D001556:Hexachlorocyclohexane; C023888:beta-hexachlorocyclohexane
keywords: Cheese whey; Decontamination efficiency; Hexachlorocyclohexane; Mixed microbial cultures; Polyhydroxyalkanoates