title: In vitro platelet aggregation and oxidative stress caused by amorphous silica nanoparticles.
abstract: Amorphous silica nanoparticles (SiNP) are being investigated for their potential use in various industrial and medical fields. Therefore, the assessment of their possible pathophysiological effect on circulating cells such as platelets is essential. We recently showed that intraperitoneal administration of SiNP causes proinflammatory and prothrombotic responses in vivo. However, little is known about the interaction of amorphous SiNP with platelets in vitro. Presently, we investigated the in vitro effects of SiNP (1, 5 and 25 μg/ml) on platelet aggregation, oxidative stress and intracellular calcium in mouse platelets. Incubation of platelets with SiNP caused a significant and dose-dependent platelet aggregation. Similarly, the activity of lactate dehydrogenase (as a marker of cell membrane integrity) was significantly increased by SiNP. Total antioxidant activity and lipid platelets vulnerability to in vitro peroxidation (measured by malondialdehyde production) were significantly increased after SiNP exposure. Additionally, SiNP exposure significantly increased the cytosolic calcium concentration. In conclusion, our in vitro data show that incubation of platelets with SiNP caused platelet aggregation, oxidative stress and increased intracellular calcium. This finding provides evidence on the toxicity of SiNP on platelet physiology.
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University P.O. Box 17666, Al Ain, UAE.
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Nemmar
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University P.O. Box 17666, Al Ain, UAE.
authors.forename: Priya
authors.initials: P
authors.lastname: Yuvaraju
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University P.O. Box 17666, Al Ain, UAE.
authors.forename: Sumaya
authors.initials: S
authors.lastname: Beegam
authors.affiliation: Department of Internal Medicine, College of Medicine and Health Sciences, United Arab Emirates University P.O. Box 17666, Al Ain, UAE.
authors.forename: Javed
authors.initials: J
authors.lastname: Yasin
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University P.O. Box 17666, Al Ain, UAE.
authors.forename: Rauda Al
authors.initials: RA
authors.lastname: Dhaheri
authors.affiliation: Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University P.O. Box 17666, Al Ain, UAE.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Fahim
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, College of Medicine & Health Sciences, Sultan Qaboos University P.O. Box 35, Muscat 123, Al-Khod, Sultanate of Oman.
authors.forename: Badreldin H
authors.initials: BH
authors.lastname: Ali
keywords: Amorphous silica nanoparticles; Oxidative stress; mice; platelets