title: Spectrophotometric methods to determine protein concentration.
abstract: Measuring the concentration of proteins is an essential part of enzyme evaluations or to monitor protein yields during protein isolation procedures. Decisions on the usefulness of any isolation procedure depend on knowing the relative concentrations of a particular protein or enzyme in relation to the concentrations of all the proteins present. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting with the protein. This review describes protein determination methods for measuring protein concentration in solution.
authors.affiliation: Biochemistry, University of KwaZulu-Natal, Pietermaritzburg, PBX01, Scottsville, 3209, South Africa, GoldringD@ukzn.ac.za.
authors.forename: J P Dean
authors.initials: JP
authors.lastname: Goldring
mesh_terms: D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D011506:Proteins; D011804:Quinolines; D012394:Rosaniline Dyes; C047117:bicinchoninic acid; C004692:coomassie Brilliant Blue