title: Experience of more than 100 preimplantation genetic diagnosis cycles for monogenetic diseases using whole genome amplification and linkage analysis in a single centre.
abstract: OBJECTIVE
  To report the outcomes of more than 100 cycles of preimplantation genetic diagnosis for monogenetic diseases.
  
  
  METHODS
  Case series.
  
  
  METHODS
  Tertiary assisted reproductive centre in Hong Kong, where patients needed to pay for the cost of preimplantation genetic diagnosis on top of standard in-vitro fertilisation charges.
  
  
  METHODS
  Patients undergoing preimplantation genetic diagnosis for monogenetic diseases at the Centre of Assisted Reproduction and Embryology, Queen Mary Hospital-The University of Hong Kong between 1 August 2007 and 30 April 2014 were included.
  
  
  METHODS
  In-vitro fertilisation, intracytoplasmic sperm injection, embryo biopsy, and preimplantation genetic diagnosis.
  
  
  METHODS
  Ongoing pregnancy rate and implantation rate.
  
  
  RESULTS
  Overall, 124 cycles of preimplantation genetic diagnosis were initiated in 76 patients, 101 cycles proceeded to preimplantation genetic diagnosis, and 92 cycles had embryo transfer. The ongoing pregnancy rate was 28.2% per initiated cycle and 38.0% per embryo transfer, giving an implantation rate of 35.2%. There were 16 frozen-thawed embryo transfer cycles in which, following preimplantation genetic diagnosis, cryopreserved embryos were replaced resulting in an ongoing pregnancy rate of 37.5% and implantation rate of 30.0%. The cumulative ongoing pregnancy rate was 33.1%. The most frequent indication for preimplantation genetic diagnosis was thalassaemia, followed by neurodegenerative disorder and cancer predisposition. There was no misdiagnosis.
  
  
  CONCLUSIONS
  Preimplantation genetic diagnosis is a reliable method to prevent couples conceiving fetuses severely affected by known genetic disorders, with ongoing pregnancy and implantation rates similar to those for in-vitro fertilisation for routine infertility treatment.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong.
authors.forename: Judy F C
authors.initials: JF
authors.lastname: Chow
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong.
authors.forename: William S B
authors.initials: WS
authors.lastname: Yeung
authors.affiliation: Department of Obstetrics and Gynaecology, Queen Mary Hospital, Hong Kong.
authors.forename: Vivian C Y
authors.initials: VC
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynaecology, Queen Mary Hospital, Hong Kong.
authors.forename: Estella Y L
authors.initials: EY
authors.lastname: Lau
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong.
authors.forename: P C
authors.initials: PC
authors.lastname: Ho
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong.
authors.forename: Ernest H Y
authors.initials: EH
authors.lastname: Ng
mesh_terms: D000328:Adult; D015925:Cryopreservation; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000706:statistics & numerical data; D005260:Female; D005307:Fertilization in Vitro / Q000706:statistics & numerical data; D020022:Genetic Predisposition to Disease / Q000196:embryology*; D005820:Genetic Testing / Q000379:methods*; D006723:Hong Kong; D006801:Humans; D019636:Neurodegenerative Diseases / Q000175:diagnosis / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods* / Q000706:statistics & numerical data; D013789:Thalassemia / Q000175:diagnosis / Q000235:genetics
keywords: Genetic diseases, inborn; Genome; Preimplantation diagnosis