title: Junction-mediating and regulatory protein (JMY) is essential for early porcine embryonic development.
abstract: Junction-mediating and regulatory protein (JMY) is a regulator of both transcription and actin filament assembly. JMY is a critical nucleation-promoting factor (NPF); however, its role in the development of mammalian embryos is poorly understood. In the current study, we investigated the functional roles of the NPF JMY in porcine embryos. Porcine embryos expressed JMY mRNA and protein, and JMY protein moved from the cytoplasm to the nucleus at later embryonic developmental stages. Knockdown of JMY by RNA interference markedly decreased the rate of blastocyst development, validating its role in the development of porcine embryos. Furthermore, injection of JMY dsRNA also impaired actin and Arp2 expression, and co-injection of actin and Arp2 mRNA partially rescued blastocyst development. Taken together, our results show that the NPF JMY is involved in the development of porcine embryos by regulating the NPF-Arp2-actin pathway.
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Zi Li
authors.initials: ZL
authors.lastname: Lin
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.forename: Suk
authors.initials: S
authors.lastname: Namgoong
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D051377:Actin-Related Protein 2 / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics / Q000378:metabolism; D021581:Active Transport, Cell Nucleus; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D004477:Ectogenesis; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques / Q000662:veterinary; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D008845:Microinjections / Q000662:veterinary; D009028:Morula / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010312:Parthenogenesis; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D034421:Sus scrofa / Q000196:embryology / Q000378:metabolism*; D015534:Trans-Activators / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051377:Actin-Related Protein 2; D000199:Actins; D009687:Nuclear Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D015534:Trans-Activators