title: Linear IgA/IgG bullous dermatosis reacts with multiple laminins and integrins.
abstract: BACKGROUND
  Since the original description by Zone et al in 1994, the disease entity and target antigens in linear IgA/IgG bullous dermatosis (LAGBD) have not been clarified in 20 years.
  
  
  OBJECTIVE
  To determine autoantibodies and autoantigens in a new LAGBD case which showed atypical clinical and histopathological findings without apparent mucosal involvement.
  
  
  METHODS
  We performed various indirect immunofluorescence and immunoblotting studies.
  
  
  RESULTS
  Indirect immunofluorescence of 1M NaCl-split skin showed IgG and IgA reactivity with both epidermal and dermal sides. Immunoblotting studies using various antigen sources revealed circulating IgG and IgA antibodies reactive with laminin-332, laminin-γ1 and integrin α6β4 in various patterns. Absorption study using recombinant proteins of laminin-γ1 indicated that the patient serum reacted with different epitopes between laminin-γ1 and laminin-γ2.
  
  
  CONCLUSIONS
  This study presented for the first time a LAGBD patient with IgG and IgA antibodies to various laminins and integrins.
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Atsunari
authors.initials: A
authors.lastname: Tsuchisaka
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Kwesi
authors.initials: K
authors.lastname: Teye
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Norito
authors.initials: N
authors.lastname: Ishii
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sogame
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Harada
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Nakagomi
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Shimada
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tateishi
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Hirako
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hashimoto
mesh_terms: D000284:Administration, Oral; D000287:Administration, Topical; D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D001707:Biopsy, Needle; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D007797:Laminin / Q000276:immunology / Q000378:metabolism*; D062027:Linear IgA Bullous Dermatosis / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology*; D008297:Male; D018570:Risk Assessment; D012720:Severity of Illness Index; D016896:Treatment Outcome
chemical_list: D000305:Adrenal Cortex Hormones; D001323:Autoantibodies; D016023:Integrins; D007797:Laminin
keywords: integrin α6β4; laminin-332; laminin-γ1; linear IgA/IgG bullous dermatosis