title: The expression of CCN2, IQSEC, RSPO1, DNAJC15, RIPK2, IL13RA2, IRS1, and IRS2 genes in blood of obese boys with insulin resistance.
abstract: The development of obesity and its metabolic complications is associated with dysregulation of various intrinsic mechanisms, which control basic metabolic processes via changes in the expression of numerous regulatory genes. We studied the expression of the subset of genes, which responsible for control of cell growth and glucose metabolism, in blood cells of obese boys with normal and impaired insulin sensitivity as well as in normal (control) individuals. It was shown that obesity with normal insulin sensitivity enhances the expression of IRS1, RIPK2, IL13RA2, RSPO1, IQSEC, and CCN2 genes but decreases the expression level IRS2 and DNAJC15 genes in the blood cells as compared to control group. Insulin resistance in obese boys leads to up-regulation of IRS2, RSPO1, and DNAJC15 gene expressions as wells to down-regulation of IRS1 and RIPK2 genes in the blood cells versus obese patients with normal insulin sensitivity. Results of this study provide evidence that obesity affects the expression of the subset of genes related to cell growth and glucose metabolism in blood cells and that insulin resistance in obesity is associated with changes in the expression level of IRS1, IRS2, RIPK2, RSPO1, and DNA JC15 genes, which contribute to the development of insulin resistance and glucose intolerance and possibly reflect some changes in fat tissue.
authors.forename: D O
authors.initials: DO
authors.lastname: Minchenko
authors.forename: V V
authors.initials: VV
authors.lastname: Davydov
authors.forename: O A
authors.initials: OA
authors.lastname: Budreiko
authors.forename: O S
authors.initials: OS
authors.lastname: Moliavko
authors.forename: D K
authors.initials: DK
authors.lastname: Kulieshova
authors.forename: O V
authors.initials: OV
authors.lastname: Tiazhka
authors.forename: O H
authors.initials: OH
authors.lastname: Minchenko
mesh_terms: D000293:Adolescent; D001773:Blood Cells / Q000378:metabolism* / Q000473:pathology; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism; D007333:Insulin Resistance; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000235:genetics / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D019699:Thrombospondins / Q000235:genetics / Q000378:metabolism; D000098907:R-Spondins
chemical_list: D001786:Blood Glucose; D055513:Connective Tissue Growth Factor; D020662:Guanine Nucleotide Exchange Factors; D050956:HSP40 Heat-Shock Proteins; D055504:Insulin Receptor Substrate Proteins; D053663:Interleukin-13 Receptor alpha2 Subunit; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D019699:Thrombospondins; C526317:CCN2 protein, human; C519405:DNAJC15 protein, human; C504483:IQSEC1 protein, human; C526292:IRS1 protein, human; C526297:IRS2 protein, human; C503243:RSPO1 protein, human; D000098907:R-Spondins; C506253:RIPK2 protein, human