title: Collagen from common minke whale (Balaenoptera acutorostrata) unesu.
abstract: Collagen was prepared from common minke whale unesu and characterised. The yield of collagen was high, about 28.4% on a wet weight basis. By SDS-PAGE and CM-Toyopearl 650M column chromatography, the collagen was classified as type I collagen. The denaturation temperature of the collagen was 31.5°C, about 6-7°C lower than that of porcine collagen. Attenuated total reflectance-FTIR analysis indicated that acid-soluble collagen from common minke whale unesu held its triple helical structure well, but the structures of porcine skin collagen and pepsin-solubilized collagen from common minke whale unesu were changed slightly, due to the loss of N- and C-terminus domains.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Agriculture, Hokkaido 0992493, Japan. Electronic address: t1nagai@bioindustry.nodai.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Nagoya Research Institute, Toyoake, Aichi 4701131, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Department of Food Science and Technology, Tokyo University of Agriculture, Hokkaido 0992493, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Nagashima
keywords: Attenuated total reflectance-Fourier transform infra-red (ATR-FTIR) analysis; Collagen; Common minke whale unesu