title: The Effect of LDL-Apheresis and Rheohaemapheresis Treatment on Vitamin E.
abstract: Lipid apheresis (extracorporeal lipoprotein elimination) is administered to patients with familial hypercholesterolemia who fail to respond to standard therapy. The nature of the treatment process raises the suspicion that it decreases not only cholesterol but also antioxidants. A group of 12 patients (average age 47±17 y, 4 homozygous and 8 heterozygous individuals) with familial hypercholesterolemia treated by LDL-apheresis or rheohaemapheresis for 3-12 y was included in the study. In addition to cholesterol and triacylglycerol levels, vitamin E and vitamin A and also other markers of antioxidant activity were investigated. Nevertheless, the most important determined parameter was the vitamin E/cholesterol ratio in serum and lipoproteins. The results indicate that both extracorporeal elimination methods are effective and suitable ways to treat severe familial hypercholesterolemia, as the LDL fraction of cholesterol decreased by approximately 77% and 66% following LDL-apheresis and rheohaemapheresis, respectively. In addition, the serum vitamin E decreased by 54% and 57% and the decrease of the serum vitamin A was approximately 20%. However, the main marker of antioxidant capacity, vitamin E/cholesterol ratio, in the serum, VLDL and LDL significantly increased. The increase of vitamin E levels in the erythrocyte membranes of 2% following LDL-apheresis and a significant increase of 4% following rheohaemapheresis were confirmed. The presented results indicate that LDL-apheresis and rheohaemapheresis can be considered to be safe procedures according to the antioxidant capacity of the serum, VLDL and LDL lipoprotein fractions and the erythrocyte membrane.
authors.affiliation: 3rd Internal Gerontometabolic Clinic, University Hospital Hradec Králové and Charles University in Prague, Faculty of Medicine in Hradec Králové
authors.forename: Dagmar
authors.initials: D
authors.lastname: Solichová
authors.forename: Milan
authors.initials: M
authors.lastname: Bláha
authors.forename: Jana
authors.initials: J
authors.lastname: Aufartová
authors.forename: Lenka Kujovská
authors.initials: LK
authors.lastname: Krcmová
authors.forename: Jirí
authors.initials: J
authors.lastname: Plíšek
authors.forename: Barbora
authors.initials: B
authors.lastname: Honegrová
authors.forename: Eva
authors.initials: E
authors.lastname: Kasalová
authors.forename: Miriam
authors.initials: M
authors.lastname: Lánská
authors.forename: Lubor
authors.initials: L
authors.lastname: Urbánek
authors.forename: Luboš
authors.initials: L
authors.lastname: Sobotka
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001781:Blood Component Removal / Q000379:methods*; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood*; D015243:Cholesterol, VLDL / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000628:therapy*; D008074:Lipoproteins / Q000097:blood; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008074:Lipoproteins; D014280:Triglycerides; D014801:Vitamin A; D014810:Vitamin E; D002784:Cholesterol