title: Vector sequence contamination of the Plasmodium vivax sequence database in PlasmoDB and In silico correction of 26 parasite sequences.
abstract: We found a 47 aa protein sequence that occurs 17 times in the Plasmodium vivax nucleotide database published on PlasmoDB. Coding sequence analysis showed multiple restriction enzyme sites within the 141 bp nucleotide sequence, and a His6 tag attached to the 3' end, suggesting cloning vector origins. Sequences with vector contamination were submitted to NCBI, and BLASTN was used to cross-examine whole-genome shotgun contigs (WGS) from four recently deposited P. vivax whole genome sequencing projects. There are at least 26 genes listed in the PlasmoDB database that incorporate this cloning vector sequence into their predicted provisional protein products.
authors.affiliation: Department of Parasitology, Bengbu Medical College, Bengbu, People's Republic of China. Bengbucity@163.com.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Tao
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Wuxi, China. uouping7@gmail.com.|Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health, Wuxi, China. uouping7@gmail.com.|Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Wuxi, China. uouping7@gmail.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Sui
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Wuxi, China. caojuncn@hotmail.com.|Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health, Wuxi, China. caojuncn@hotmail.com.|Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Wuxi, China. caojuncn@hotmail.com.
authors.forename: Cao
authors.initials: C
authors.lastname: Jun
authors.affiliation: Malaria Unit, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan. richard.culleton@gmail.com.
authors.forename: Richard
authors.initials: R
authors.lastname: Culleton
authors.affiliation: Department of Parasitology, Bengbu Medical College, Bengbu, People's Republic of China. fq333@sohu.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Department of Parasitology, Bengbu Medical College, Bengbu, People's Republic of China. xiahui912@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Wuxi, China. gaoqi54@hotmail.com.|Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health, Wuxi, China. gaoqi54@hotmail.com.|Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Wuxi, China. gaoqi54@hotmail.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D001483:Base Sequence; D003198:Computer Simulation; D030561:Databases, Nucleic Acid; D005822:Genetic Vectors / Q000235:genetics*; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000145:classification / Q000235:genetics*