title: Computational Prediction of acyl-coA Binding Proteins Structure in Brassica napus.
abstract: Acyl-coA binding proteins could transport acyl-coA esters from plastid to endoplasmic reticulum, prior to fatty acid biosynthesis, leading to the formation of triacylglycerol. The structure and the subcellular localization of acyl-coA binding proteins (ACBP) in Brassica napus were computationally predicted in this study. Earlier, the structure analysis of ACBPs was limited to the small ACBPs, the current study focused on all four classes of ACBPs. Physicochemical parameters including the size and the length, the intron-exon structure, the isoelectric point, the hydrophobicity, and the amino acid composition were studied. Furthermore, identification of conserved residues and conserved domains were carried out. Secondary structure and tertiary structure of ACBPs were also studied. Finally, subcellular localization of ACBPs was predicted. The findings indicated that the physicochemical parameters and subcellular localizations of ACBPs in Brassica napus were identical to Arabidopsis thaliana. Conserved domain analysis indicated that ACBPs contain two or three kelch domains that belong to different families. Identical residues in acyl-coA binding domains corresponded to eight amino acid residues in all ACBPs of B. napus. However, conserved residues of common ACBPs in all species of animal, plant, bacteria and fungi were only inclusive in small ACBPs. Alpha-helixes were displayed and conserved in all the acyl-coA binding domains, representing almost the half of the protein structure. The findings confirm high similarities in ACBPs between A. thaliana and B. napus, they might share the same functions but loss or gain might be possible.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang, 435599, China.
authors.forename: Nadia Haingotiana
authors.initials: NH
authors.lastname: Raboanatahiry
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei, 430062, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Lu
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang, 435599, China.
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D029688:Brassica napus / Q000737:chemistry* / Q000378:metabolism; D017124:Conserved Sequence; D026261:Diazepam Binding Inhibitor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D026261:Diazepam Binding Inhibitor; D010940:Plant Proteins