title: Unraveling adaptation of Pontibacter korlensis to radiation and infertility in desert through complete genome and comparative transcriptomic analysis.
abstract: The desert is a harsh habitat for flora and microbial life due to its aridness and strong radiation. In this study, we constructed the first complete and deeply annotated genome of the genus Pontibacter (Pontibacter korlensis X14-1(T) = CCTCC AB 206081(T), X14-1). Reconstruction of the sugar metabolism process indicated that strain X14-1 can utilize diverse sugars, including cellulose, starch and sucrose; this result is consistent with previous experiments. Strain X14-1 is also able to resist desiccation and radiation in the desert through well-armed systems related to DNA repair, radical oxygen species (ROS) detoxification and the OstAB and TreYZ pathways for trehalose synthesis. A comparative transcriptomic analysis under gamma radiation revealed that strain X14-1 presents high-efficacy operating responses to radiation, including the robust expression of catalase and the manganese transport protein. Evaluation of 73 novel genes that are differentially expressed showed that some of these genes may contribute to the strain's adaptation to radiation and desiccation through ferric transport and preservation.
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.affiliation: BGI Shenzhen, Shenzhen 518083, China.
authors.forename: Wenkui
authors.initials: W
authors.lastname: Dai
authors.affiliation: BGI Shenzhen, Shenzhen 518083, China.
authors.forename: Chuangzhao
authors.initials: C
authors.lastname: Qiu
authors.affiliation: BGI Shenzhen, Shenzhen 518083, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Mengzhou
authors.initials: M
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Fang
authors.affiliation: BGI Shenzhen, Shenzhen 518083, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: East China Sea fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: BGI Yunnan, Kunming 650228, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
mesh_terms: D000222:Adaptation, Physiological; D003589:Cytophaga / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D018745:Genome, Plant; D007246:Infertility / Q000235:genetics / Q000503:physiopathology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D059467:Transcriptome