title: Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill.
abstract: Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.
authors.affiliation: Centro de Desarrollo de Productos Bióticos del IPN, Km 8.5 carr, Yautepec-Jojutla, colonia San Isidro, apartado postal 24, 62731 Yautepec, Morelos, Mexico. Electronic address: aniurka10@hotmail.com.
authors.forename: A N
authors.initials: AN
authors.lastname: Hernández-Lauzardo
authors.affiliation: Centro de Desarrollo de Productos Bióticos del IPN, Km 8.5 carr, Yautepec-Jojutla, colonia San Isidro, apartado postal 24, 62731 Yautepec, Morelos, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Bautista-Baños
authors.affiliation: Centro de Desarrollo de Productos Bióticos del IPN, Km 8.5 carr, Yautepec-Jojutla, colonia San Isidro, apartado postal 24, 62731 Yautepec, Morelos, Mexico.
authors.forename: M G
authors.initials: MG
authors.lastname: Velázquez-Del Valle
authors.affiliation: Centro de Desarrollo de Productos Bióticos del IPN, Km 8.5 carr, Yautepec-Jojutla, colonia San Isidro, apartado postal 24, 62731 Yautepec, Morelos, Mexico.
authors.forename: M G
authors.initials: MG
authors.lastname: Méndez-Montealvo
authors.affiliation: Centro de Desarrollo de Productos Bióticos del IPN, Km 8.5 carr, Yautepec-Jojutla, colonia San Isidro, apartado postal 24, 62731 Yautepec, Morelos, Mexico.
authors.forename: M M
authors.initials: MM
authors.lastname: Sánchez-Rivera
authors.affiliation: Centro de Desarrollo de Productos Bióticos del IPN, Km 8.5 carr, Yautepec-Jojutla, colonia San Isidro, apartado postal 24, 62731 Yautepec, Morelos, Mexico.
authors.forename: L A
authors.initials: LA
authors.lastname: Bello-Pérez
keywords: Antifungal activity; Chitosan; Image analysis; Spore ornamentations