title: Insights into the origin of the nuclear localization signals in conserved ribosomal proteins.
abstract: Eukaryotic ribosomal proteins, unlike their bacterial homologues, possess nuclear localization signals (NLSs) to enter the cell nucleus during ribosome assembly. Here we provide a comprehensive comparison of bacterial and eukaryotic ribosomes to show that NLSs appear in conserved ribosomal proteins via remodelling of their RNA-binding domains. This finding enabled us to identify previously unknown NLSs in ribosomal proteins from humans, and suggests that, apart from promoting protein transport, NLSs may facilitate folding of ribosomal RNA.
authors.affiliation: 1] Strasbourg University, 4 Rue Blaise Pascal, 67081 Strasbourg, France [2] Institute of Genetics and Molecular and Cellular Biology, 1 Rue Laurent Fries, 67400 Illkirch-Graffenstaden, France [3] Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06511, USA.
authors.forename: Sergey
authors.initials: S
authors.lastname: Melnikov
authors.affiliation: 1] Strasbourg University, 4 Rue Blaise Pascal, 67081 Strasbourg, France [2] Institute of Genetics and Molecular and Cellular Biology, 1 Rue Laurent Fries, 67400 Illkirch-Graffenstaden, France.
authors.forename: Adam
authors.initials: A
authors.lastname: Ben-Shem
authors.affiliation: 1] Strasbourg University, 4 Rue Blaise Pascal, 67081 Strasbourg, France [2] Institute of Genetics and Molecular and Cellular Biology, 1 Rue Laurent Fries, 67400 Illkirch-Graffenstaden, France.
authors.forename: Gulnara
authors.initials: G
authors.lastname: Yusupova
authors.affiliation: 1] Strasbourg University, 4 Rue Blaise Pascal, 67081 Strasbourg, France [2] Institute of Genetics and Molecular and Cellular Biology, 1 Rue Laurent Fries, 67400 Illkirch-Graffenstaden, France [3] CNRS, 1 Rue Laurent Fries, 67400 Illkirch-Graffenstaden, France.
authors.forename: Marat
authors.initials: M
authors.lastname: Yusupov
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism*; D017124:Conserved Sequence; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000378:metabolism*; D021381:Protein Transport; D012335:RNA, Ribosomal / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D019913:Nuclear Localization Signals; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins