title: Scaling-up the synthesis of myristate glucose ester catalyzed by a CALB-displaying Pichia pastoris whole-cell biocatalyst.
abstract: The novel whole-cell biocatalyst Candida antarctica lipase B displaying-Pichia pastoris (Pp-CALB) is characterized by its low preparation cost and could be an alternative to the commercial immobilized Candida antarctica lipase B (CALB). This study addresses the feasibility of using Pp-CALB in large scale glucose fatty acid esters production. 1,2-O-Isopropylidene-α-D-glucofuranose (IpGlc) was used as the acyl acceptor to overcome the low solubility of glucose in an organic solvent and to avoid the addition of toxic co-solvents. IpGlc significantly improved the Pp-CALB catalyzing esterification efficiency when using long chain fatty acids as the acyl donor. Under the preferred operating conditions (50 °C, 40 g/L molecular sieve dosage and 200 rpm mixing intensity), 60.5% of IpGlc converted to 6-O-myristate-1, 2-O-isopropylidene-α-D-glucofuranose (C14-IpGlc) after a 96-h reaction in a 2-L stirred reactor. In a 5-L pilot scale test, Pp-CALB also showed a similar substrate conversion rate of 55.4% and excellent operational stability. After C14-IpGlc was collected, 70% trifluoroacetic acid was adopted to hydrolyze C14-IpGlc to myristate glucose ester (C14-Glc) with a high yield of 95.3%. In conclusion, Pp-CALB is a powerful biocatalyst available for industrial synthesis, and this study describes an applicable and economical process for the large scale production of myristate glucose ester.
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: DongHeng
authors.initials: D
authors.lastname: Guo
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: YanShan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: syhan@scut.edu.cn.
authors.forename: ShuangYan
authors.initials: S
authors.lastname: Han
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: spzheng@scut.edu.cn.
authors.forename: SuiPing
authors.initials: S
authors.lastname: Zheng
mesh_terms: D055162:Biocatalysis; D019149:Bioreactors / Q000382:microbiology; D002175:Candida / Q000201:enzymology; D004951:Esterification; D005240:Feasibility Studies; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D009005:Monosaccharides / Q000378:metabolism; D009226:Myristates / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D012997:Solvents
chemical_list: C516585:1,2-O-isopropylidene-alpha-D-glucofuranose; D005656:Fungal Proteins; D009005:Monosaccharides; D009226:Myristates; D011994:Recombinant Proteins; D012997:Solvents; D008049:Lipase; C431349:lipase B, Candida antarctica; D005947:Glucose
keywords: 1,2-O-Isopropylidene-α-d-glucofuranose; Candida antarctica lipase B; Myristate glucose ester; Scale synthesis; Yeast whole cell biocatalyst