title: Western blotting: remembrance of things past.
abstract: Western blotting sprung from the need to develop a sensitive visual assay for the antigen specificity of monoclonal antibodies. The technique employed SDS-PAGE of protein antigens, electrophoretic replica transfer of gel-resolved proteins to unmodified nitrocellulose sheets, probing the immobilized antigens with hybridomas, and detection of antibody-antigen complexes with radiolabeled staphylococcal protein A and autoradiography. The simplicity and relevance of the method have led to its expansive application as an immunodiagnostic and a ubiquitous research tool in biology and medicine.
authors.affiliation: Molecular Pharmaceutics Corporation, 85423 Dudley Drive, Chapel Hill, NC, 27517, USA, burnetten@iname.com.
authors.forename: W Neal
authors.initials: WN
authors.lastname: Burnette
mesh_terms: D015153:Blotting, Western / Q000266:history*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans