title: Dengue virus detection in Aedes aegypti larvae from southeastern Brazil.
abstract: The transmission of dengue, the most important arthropod-borne viral disease in Brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its Aedes vectors. In the present study, we mapped dengue vectors in Ouro Preto and Ouro Branco, Minas Gerais, by installing ovitraps in 32 public schools. The traps were examined monthly between September, 2011 through July, 2012 and November, 2012 to April, 2013. The larvae were reared until the fourth stadium and identified according to species. The presence of dengue virus was detected by real time PCR and agarose gel electrophoresis. A total of 1,945 eggs was collected during the 17 months of the study. The Ovitrap Positivity Index (OPI) ranged from 0 to 28.13% and the Eggs Density Index (EDI) ranged from 0 to 59.9. The predominant species was Aedes aegypti, with 84.9% of the hatched larvae. Although the collection was low when compared to other ovitraps studies, vertical transmission could be detected. Of the 54 pools, dengue virus was detected in four Ae. aegypti pools.
authors.affiliation: Laboratório de Microbiologia Geral e Enzimologia. samyracecilio@gmail.com.
authors.forename: Samyra Giarola
authors.initials: SG
authors.lastname: Cecílio
authors.affiliation: Laboratório de Microbiologia Geral e Enzimologia.
authors.forename: Willer Ferreira Silva
authors.initials: WF
authors.lastname: Júnior
authors.affiliation: Laboratório de Bioquímica e Imunologia Celular e Molecular, Departamento de Química, Biotecnologia e Engenharia de Bioprocessos (DQBIO/Campus Alto Paraopeba/UFSJ), Ouro Branco, MG, Brazil.
authors.forename: Antônio Helvécio
authors.initials: AH
authors.lastname: Tótola
authors.affiliation: Laboratório de Biologia e Tecnologia de Micro-organismos, Departamento de Ciências Biológicas, (DCB/UFOP), Ouro Preto, MG, Brazil.
authors.forename: Cíntia Lopes
authors.initials: CL
authors.lastname: de Brito Magalhães
authors.affiliation: Laboratório de Microbiologia (Campus Centro Oeste Dona Lindu/UFSJ), Divinópolis, MG, Brazil.
authors.forename: Jaqueline Maria Siqueira
authors.initials: JM
authors.lastname: Ferreira
authors.affiliation: Laboratório de Microbiologia Geral e Enzimologia.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: de Magalhães
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001938:Brazil; D003715:Dengue / Q000635:transmission; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D005260:Female; D018445:Infectious Disease Transmission, Vertical; D007303:Insect Vectors / Q000821:virology; D007814:Larva / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction
keywords: Aedes; dengue virus; mosquitoes; oviposition; ovitraps; transovarial transmission