title: Liquid Extraction Surface Analysis Mass Spectrometry Coupled with Field Asymmetric Waveform Ion Mobility Spectrometry for Analysis of Intact Proteins from Biological Substrates.
abstract: Previously we have shown that liquid extraction surface analysis (LESA) mass spectrometry is suitable for the analysis of intact proteins from a range of biological substrates. Here we show that LESA mass spectrometry may be coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for top-down protein analysis directly from thin tissue sections (mouse liver, mouse brain) and from bacterial colonies (Escherichia coli) growing on agar. Incorporation of FAIMS results in significant improvements in signal-to-noise and reduced analysis time. Abundant protein signals are observed in single scan mass spectra. In addition, FAIMS enables gas-phase separation of molecular classes, for example, lipids and proteins, enabling improved analysis of both sets of species from a single LESA extraction.
authors.affiliation: †School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Joscelyn
authors.initials: J
authors.lastname: Sarsby
authors.affiliation: †School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Rian L
authors.initials: RL
authors.lastname: Griffiths
authors.affiliation: ‡National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, United Kingdom.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Race
authors.affiliation: ‡National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, United Kingdom.|§School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.
authors.forename: Josephine
authors.initials: J
authors.lastname: Bunch
authors.affiliation: †School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Randall
authors.affiliation: †School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Creese
authors.affiliation: †School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000818:Animals; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D011506:Proteins / Q000032:analysis*; D013499:Surface Properties
chemical_list: D011506:Proteins