title: Arabidopsis thaliana KORRIGAN1 protein: N-glycan modification, localization, and function in cellulose biosynthesis and osmotic stress responses.
abstract: Plant cellulose biosynthesis is a complex process involving cellulose-synthase complexes (CSCs) and various auxiliary factors essential for proper orientation and crystallinity of cellulose microfibrils in the apoplast. Among them is KORRIGAN1 (KOR1), a type-II membrane protein with multiple N-glycans within its C-terminal cellulase domain. N-glycosylation of the cellulase domain was important for KOR1 targeting to and retention within the trans-Golgi network (TGN), and prevented accumulation of KOR1 at tonoplasts. The degree of successful TGN localization of KOR1 agreed well with in vivo-complementation efficacy of the rsw2-1 mutant, suggesting non-catalytic functions in the TGN. A dynamic interaction network involving microtubules, CSCs, KOR1, and currently unidentified glycoprotein component(s) likely determines stress-triggered re-organization of cellulose biosynthesis and resumption of cell-wall growth under stress.
authors.affiliation: a Molekulare Physiologie der Pflanzen; Institut für Biologie & Biotechnologie der Pflanzen; Westfälische Wilhelms-Universität Münster ; Münster , Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: von Schaewen
authors.forename: Stephan
authors.initials: S
authors.lastname: Rips
authors.forename: In Sil
authors.initials: IS
authors.lastname: Jeong
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koiwa
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D006031:Glycosylation; D008565:Membrane Proteins / Q000378:metabolism*; D009997:Osmotic Pressure; D011134:Polysaccharides / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C429844:KOR1 protein, Arabidopsis; D008565:Membrane Proteins; D011134:Polysaccharides; D002482:Cellulose; D002480:Cellulase
keywords: 4-endoglucanase; Arabidopsis; CSC, cellulose-synthase complex; ER, endoplasmic reticulum; GHF9, glycosyl-hydrolase family 9; PM, plasma membrane; TGN, trans-Golgi network; cell wall; complex N-glycans; trans-Golgi network; β1,