title: Increased Levels of 8-Isoprostane in EBC of NO2-Exposed Rats.
abstract: Several epidemiological studies have shown the impact on respiratory health of pollution of nitrogen dioxide (NO2), particulate matter (PM10), and ozone (O3) as an environmental mixture. However, the influence of individual components of airborne pollutants is less well known. Our study examined the cumulative effects of a single pollutant, NO2, on sensitized rats by measurement of isoprostane release in exhaled breath condensate (EBC). Three groups of six rats were used: (1) controls (only exposed to air), (2) sensitized and challenged by ovalbumin and exposed to air, and (3) sensitized, challenged by ovalbumin, and exposed to NO(2). There was no marked change in 8-isoprostane levels in EBC of sensitized rats, whereas a significant increase of 8-isoprostane was found in rats sensitized and exposed to NO2. Data indicate effect of exposure to NO2 is evident as increased 8-isoprostane levels in EBC, a relevant marker for assessment of pulmonary inflammation or oxidant stress and conventionally found in EBC of asthmatic subjects.
authors.affiliation: a CHU de Lille , Lille , France.
authors.forename: Virginie
authors.initials: V
authors.lastname: de Broucker
authors.forename: Sebastien
authors.initials: S
authors.lastname: Hulo
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cherot-Kornobis
authors.forename: Annie
authors.initials: A
authors.lastname: Sobaszek
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Edme
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests / Q000379:methods*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D008297:Male; D009585:Nitrogen Dioxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D052638:Particulate Matter / Q000633:toxicity*; D011014:Pneumonia / Q000139:chemically induced; D051381:Rats
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D052638:Particulate Matter; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D009585:Nitrogen Dioxide