title: High-resolution crystal structure of the leucine-rich repeat domain of the human tumour suppressor PP32A (ANP32A).
abstract: Acidic leucine-rich nuclear phosphoprotein 32A (PP32A) is a tumour suppressor whose expression is altered in many cancers. It is an apoptotic enhancer that stimulates apoptosome-mediated caspase activation and also forms part of a complex involved in caspase-independent apoptosis (the SET complex). Crystals of a fragment of human PP32A corresponding to the leucine-rich repeat domain, a widespread motif suitable for protein-protein interactions, have been obtained. The structure has been refined to 1.56 Å resolution. This domain was previously solved at 2.4 and 2.69 Å resolution (PDB entries 2je0 and 2je1, respectively). The new high-resolution structure shows some differences from previous models: there is a small displacement in the turn connecting the first α-helix (α1) to the first β-strand (β1), which slightly changes the position of α1 in the structure. The shift in the turn is observed in the context of a new crystal packing unrelated to those of previous structures.
authors.affiliation: Department of Genomics and Proteomics, Instituto de Biomedicina de Valencia - CSIC, Jaime Roig 11, 46010 Valencia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Zamora-Caballero
authors.affiliation: Department of Genomics and Proteomics, Instituto de Biomedicina de Valencia - CSIC, Jaime Roig 11, 46010 Valencia, Spain.
authors.forename: Lina
authors.initials: L
authors.lastname: Šiaučiunaite-Gaubard
authors.affiliation: Department of Genomics and Proteomics, Instituto de Biomedicina de Valencia - CSIC, Jaime Roig 11, 46010 Valencia, Spain.
authors.forename: Jeronimo
authors.initials: J
authors.lastname: Bravo
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics; D007930:Leucine / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: C087425:ANP32A protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D007930:Leucine
keywords: PP32A LRR domain structure; SET complex; tumour suppressor