title: Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets.
abstract: The effects of crude protein (CP) levels in the diet on the mRNA expression of amino acid (AA) transporters were studied in a 45-d trial. Eighteen piglets with an initial body weight (BW) of 9.57 kg were assigned to three groups (14%, 17%, and 20% CP in the diet) in a completely randomized design (six replicates per treatment). Diets were supplemented with crystalline AA to achieve equal standardized ileal digestible contents of Lys, Met plus Cys, Thr, and Trp, and were provided ad libitum. After 45 d, all piglets were slaughtered to collect small intestine samples. Compared with the values in the 14% CP group, the expressions of ASCT2, 4F2hc, and ATB(0) mRNA in the jejunum were increased by 23.00%, 12.00%, 6.00% and 48.00%, 47.00%, 56.00% in the 17% and 20% CP groups, respectively. These results indicate that a 14% CP diet supplemented with crystalline AA may not transport enough AA into the body and maintain growth performance of piglets. However, a reduction of dietary 17% CP may reduce the excretion of nitrogen into the environment while supporting the development of piglets. Therefore, the 17% CP level is more suitable than 14% CP level.
authors.affiliation: Research Center of Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Liu-qin
authors.initials: LQ
authors.lastname: He
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Cui
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Kang
authors.initials: K
authors.lastname: Yao
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Li
mesh_terms: D026905:Amino Acid Transport Systems / Q000378:metabolism*; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology; D000818:Animals; D018753:Diet, Protein-Restricted / Q000379:methods*; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D007421:Intestine, Small / Q000378:metabolism*; D013552:Swine / Q000254:growth & development* / Q000378:metabolism*; D014886:Weaning
chemical_list: D026905:Amino Acid Transport Systems; D004044:Dietary Proteins
keywords: Amino acid balance; Amino acid transporters; Crude protein