title: Discordance between protein and transcript levels detected by selected reaction monitoring.
abstract: Expression levels between transcript and protein are not always correlated. In the present study, the abundance of protein PDR9/ABCG37 in 3 Arabidopsis pdr9/abcg37 mutant alleles was evaluated using selected reaction monitoring analysis. The results showed that protein and mRNA expression levels were similar in 2 mutant alleles. The mRNA expression levels in another mutant, determined by both semi-quantitative and quantitative RT-PCR, were similar to the wild-type, although the abundance of protein was about half the abundance of the wild-type. These results suggested that using only mRNA expression levels to infer protein abundance, compare mutants or responses to various stimuli may lead to incorrect interpretation and conclusions.
authors.affiliation: a Plant Global Education Project; Graduate School of Biological Sciences; Nara Institute of Science and Technology ; Takayama , Ikoma , Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Fukao
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007501:Iron / Q000378:metabolism*
chemical_list: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; C515019:PDR9 protein, Arabidopsis; D007501:Iron
keywords: Arabidopsis thaliana; Fe, iron; LC/MS, high performance liquid chromatography coupled with mass spectrometry; PDR9/ABCG37; SRM analysis; SRM, selected reaction monitoring; absolute protein quantification; iTRAQ, isobaric tags for relative and absolute quantification; mass spectrometry