title: Methylation of KvDMR1 involved in regulating the imprinting of CDKN1C gene in cattle.
abstract: The CDKN1C gene encodes a cyclin-dependent kinase inhibitor and is one of the key genes involved in the development of Beckwith-Wiedemann syndrome and cancer. In this study, using a direct sequencing approach based on a single nucleotide polymorphism (SNP) at genomic DNA and cDNA levels, we show that CDKN1C exhibits monoallelic expression in all seven studied organs (heart, liver, spleen, lung, kidney, muscle and subcutaneous fat) in cattle. To investigate how methylation regulates imprinting of CDKN1C in cattle, allele-specific methylation patterns in two putative differential methylation regions (DMRs), the CDKN1C DMR and KvDMR1, were analyzed in three tissues (liver, spleen and lung) using bisulfite sequencing PCR. Our results show that in the CDKN1C DMR both parental alleles were unmethylated in all three analyzed tissues. In contrast, KvDMR1 was differentially methylated between the two parental alleles in the same tissues. Statistical analysis showed that there is a significant difference in the methylation level between the two parental alleles (P < 0.01), confirming that this region is the DMR of KvDMR1 and that it may be correlated with CDKN1C imprinting.
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Mengnan
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Science and Life Engineering, Science and Technology, University of Hebei, Shijiazhuang, 050018, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Cui
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics*; D019175:DNA Methylation; D018392:Genomic Imprinting; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D050761:Cyclin-Dependent Kinase Inhibitor p57; D024642:Potassium Channels, Voltage-Gated
keywords: allele-specific DNA methylation; allele-specific expression; bovine; cyclin-dependent kinase inhibitor; differentially methylated region; imprinting status