title: Antibacterial and hemolysis activity of polypyrrole nanotubes decorated with silver nanoparticles by an in-situ reduction process.
abstract: Polypyrrole nanotube-silver nanoparticle nanocomposites (PPy-NTs:Ag-NPs) have been synthesized by in-situ reduction of silver nitrate (AgNO3) to suppress the agglomeration of Ag-NPs. The morphology and chemical structure of the nanocomposites have been studied by HRTEM, SEM, XRD, FTIR and UV-vis spectroscopy. The average diameter of the polypyrrole nanotubes (PPy-NTs) is measured to be 130.59±5.5 nm with their length in the micrometer range, while the silver nanoparticles (Ag-NPs) exhibit spherical shape with an average diameter of 23.12±3.23 nm. In-vitro blood compatibility of the nanocomposites has been carried out via hemolysis assay. Antimicrobial activity of the nanocomposites has been investigated with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. The results depict that the hemolysis and antimicrobial activities of the nanocomposites increase with increasing Ag-NP concentration that can be controlled by the AgNO3 precursor concentration in the in-situ process.
authors.affiliation: Department of Physics, Tezpur University, Napaam, Tezpur 784028, Assam, India.
authors.forename: J
authors.initials: J
authors.lastname: Upadhyay
authors.affiliation: Department of Physics, Tezpur University, Napaam, Tezpur 784028, Assam, India. Electronic address: ask@tezu.ernet.in.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784028, Assam, India.
authors.forename: B
authors.initials: B
authors.lastname: Gogoi
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784028, Assam, India; Dibrugarh University, Dibrugarh 786004, Assam, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Buragohain
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006461:Hemolysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011758:Pyrroles / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D012834:Silver; D012835:Silver Nitrate
keywords: Antibacterial activity; Hemolysis activity; Nanocomposites; Polypyrrole nanotubes; Silver nanoparticles