title: Biocompatible surgical meshes based on decellularized human amniotic membrane.
abstract: Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM's (dHAM) in vitro cytocompatibility. The in vivo test was performed via implantation at rabbits' uterus with clinic polypropylene mesh (PP) as the control. The results indicated that dHAM possessed good biocompatibility and will be a potential candidate for biological mesh.
authors.affiliation: The Medical School, Ningbo University, Ningbo 315211, China.
authors.forename: Peina
authors.initials: P
authors.lastname: Shi
authors.affiliation: The Medical School, Ningbo University, Ningbo 315211, China.
authors.forename: Mengna
authors.initials: M
authors.lastname: Gao
authors.affiliation: The Medical School, Ningbo University, Ningbo 315211, China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Shen
authors.affiliation: The Medical School, Ningbo University, Ningbo 315211, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.affiliation: The Medical School, Ningbo University, Ningbo 315211, China. Electronic address: zhuyabin@nbu.edu.cn.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Obstetrics and Gynecology Department of the Sixth Hospital, Shanghai Jiaotong University, Shanghai 201306, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000650:Amnion / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000378:metabolism; D005109:Extracellular Matrix; D006801:Humans; D008049:Lipase / Q000378:metabolism; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D019736:Prostheses and Implants; D011817:Rabbits; D013526:Surgical Mesh
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011126:Polypropylenes; D011140:Polyurethanes; C081821:microthane foam; D003851:Deoxyribonucleases; D008049:Lipase
keywords: Biocompatibility; Biological mesh; Decellularization; Human amniotic membrane