title: Polyelectrolyte complex containing silver nanoparticles with antitumor property on Caco-2 colon cancer cells.
abstract: Polyelectrolyte complex (beads) based on N,N,N-trimethyl chitosan/alginate was successful obtained and silver nanoparticles (AgNPs) were loaded within beads. In vitro cytotoxicity assays using beads/silver nanoparticles (beads/AgNPs) provided results, indicating that this material significantly inhibited the growth of colon cancer cells (Caco-2). In vitro release studies showed that the beads stabilized AgNPs and repressed Ag(0) oxidation under gastric conditions (pH 2.0). On the other hand, at physiological condition (pH 7.4) the beads/AgNPs released 3.3 μg of Ag(+) per each beads milligram. These studies showed that the concentration of Ag(+) released (3.3 μg) was cytotoxic for the Caco-2 cells and was not cytotoxic on healthy VERO cells. This result opens new perspectives for the manufacture of biomaterials based on beads/AgNPs with anti-tumor properties.
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brazil; Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil. Electronic address: afmartins50@yahoo.com.br.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Heveline D M
authors.initials: HD
authors.lastname: Follmann
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brazil; Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Johny P
authors.initials: JP
authors.lastname: Monteiro
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brazil; Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Elton G
authors.initials: EG
authors.lastname: Bonafé
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Samara
authors.initials: S
authors.lastname: Nocchi
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Cleiser T P
authors.initials: CT
authors.lastname: Silva
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Emerson M
authors.initials: EM
authors.lastname: Girotto
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000737:chemistry; D018938:Caco-2 Cells; D048271:Chitosan / Q000737:chemistry*; D002522:Chlorocebus aethiops; D065546:Drug Liberation; D005750:Gastric Juice / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008863:Microspheres; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013045:Species Specificity; D014709:Vero Cells
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; C118071:N-trimethyl chitosan chloride; D012834:Silver; D048271:Chitosan
keywords: Alginate; N,N,N-Trimethyl chitosan; Polyelectrolyte complex; Silver nanoparticles