title: Characterization and antitumor activities of a water-soluble polysaccharide from Ampelopsis megalophylla.
abstract: A water-soluble polysaccharide named as AMP was isolated and purified from the leaves of Ampelopsis megalophylla by DEAE-52 Cellulose and Sephadex G-100 column chromatography. AMP had an average molecular weight of about 8.4 × 10(4)Da and was composed of galactose (Gal), mannose (Man), glucose (Glc), arabinose (Ara), and rhamnose (Rha) in a molar ratio of 2.7:1.6:1.1:0.6:0.3. After 10 days of AMP (50, 100, and 200mg/kg) treatment once daily in tumor-bearing mice, AMP oral administration could inhibit the growth of transplantable Sarcoma 180 (S180) tumor in mice and increase the spleen index and body weight. Furthermore, AMP also promote splenocytes' proliferation induced by concanavalin A (ConA) and lipopolysaccharide (LPS), strengthen peritoneal macrophages to devour neutral red and increase the production of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) in serum. These results suggest that AMP had clear antitumor activity, which might be related to its regulation of immune function in mice.
authors.affiliation: Department of Pharmacology, Basic Medical School, Wuhan University, Wuhan, Wuhan 430072, PR China. Electronic address: xiexianfeiut@gmail.com.
authors.forename: Xianfei
authors.initials: X
authors.lastname: Xie
authors.affiliation: College of Pharmacy, Huazhong University of Science and Technology, Wuhan, Wuhan 430030, PR China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Huazhong University of Science and Technology, Wuhan, Wuhan 430030, PR China.
authors.forename: Hanping
authors.initials: H
authors.lastname: Zhang
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014867:Water / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D007371:Interferon-gamma
keywords: Ampelopsis megalophylla; Antitumor; Immunomodulation; Polysaccharides