title: Bone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin-Biotin Binding System.
abstract: Highly porous and interconnected scaffolds were fabricated using calcium phosphate glass (CPG) for bone tissue engineering. An avidin-biotin binding system was used to improve osteoblast-like cell adhesion to the scaffold. The scaffolds had open macro- and micro-scale pores, and continuous struts without cracks or defects. Scaffolds prepared using a mixture (amorphous and crystalline CPG) were stronger than amorphous group and crystalline group. Cell adhesion assays showed that more cells adhered, with increasing cell seeding efficiency to the avidin-adsorbed scaffolds, and that cell attachment to the highly porous scaffolds significantly differed between avidin-adsorbed scaffolds and other scaffolds. Proliferation was also significantly higher for avidin-adsorbed scaffolds. Osteoblastic differentiation of MG-63 cells was observed at 3 days, and MG-63 cells in direct contact with avidin-adsorbed scaffolds were positive for type I collagen, osteopontin, and alkaline phosphatase gene expression. Osteocalcin expression was observed in the avidin-adsorbed scaffolds at 7 days, indicating that cell differentiation in avidin-adsorbed scaffolds occurred faster than the other scaffolds. Thus, these CPG scaffolds have excellent biological properties suitable for use in bone tissue engineering.
authors.affiliation: Department of Applied Life Science, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Korea.
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Hong
authors.affiliation: Department of Applied Life Science, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Korea.
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Heon-Jin
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Research Center for Oral Disease Regulation of the Aged, Chosun University School of Dentistry, 309 Pilmun-daero, Dong-gu, Gwangju, 501-759, Korea.
authors.forename: Yeong-Mu
authors.initials: YM
authors.lastname: Ko
authors.affiliation: Research Center for Oral Disease Regulation of the Aged, Chosun University School of Dentistry, 309 Pilmun-daero, Dong-gu, Gwangju, 501-759, Korea. leeyk0129@naver.com.
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Lee
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D001842:Bone and Bones; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D005898:Glass / Q000737:chemistry; D006801:Humans; D011140:Polyurethanes / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D002130:Calcium Phosphates; D011140:Polyurethanes; D001360:Avidin; D001710:Biotin; C020243:calcium phosphate
keywords: Avidin–biotin binding system; Bone tissue engineering; Calcium phosphate glass; Scaffold