title: Studying host-microbiota mutualism in Drosophila: Harnessing the power of gnotobiotic flies.
abstract: The complex interaction between the metazoan host and its commensal gut microbiota is one of the essential features of symbiosis in the animal kingdom. As there is a burgeoning interest to decipher the molecular dialog that shapes host-microbiota mutualism, the use of gnotobiotic model organism becomes an imperative approach to unambiguously parse the specific contributions to such interaction from the microbiome. In this review, we focus on several remarkable gnotobiotic studies in Drosophila that functionally depicted how the gut microbes can alter host physiology and behavior through transcriptomic regulation, hormonal control, and diet modification. These results in concert illustrate that the gnotobiotic flies mono- or poly-associated with members of its gut microbiota deliver a versatile and powerful model that is amenable to different types of studies ranging from classic genetics to large-scale systems approaches.
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon (IGFL), Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Dali
authors.initials: D
authors.lastname: Ma
authors.forename: Gilles
authors.initials: G
authors.lastname: Storelli
authors.forename: Mélanie
authors.initials: M
authors.lastname: Mitchell
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon (IGFL), Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: François
authors.initials: F
authors.lastname: Leulier
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D004330:Drosophila; D004331:Drosophila melanogaster / Q000254:growth & development; D041981:Gastrointestinal Tract / Q000378:metabolism*; D005856:Germ-Free Life / Q000502:physiology*; D006801:Humans; D064307:Microbiota / Q000502:physiology*; D013559:Symbiosis / Q000502:physiology*