title: HORMONE MEASUREMENT GUIDELINES: Tracing lipid metabolism: the value of stable isotopes.
abstract: Labelling molecules with stable isotopes to create tracers has become a gold-standard method to study the metabolism of lipids and lipoproteins in humans. There are a range of techniques which use stable isotopes to measure fatty acid flux and oxidation, hepatic fatty synthesis, cholesterol absorption and synthesis and lipoprotein metabolism in humans. Stable isotope tracers are safe to use, enabling repeated studies to be undertaken and allowing studies to be undertaken in children and pregnant women. This review provides details of the most appropriate tracers to use, the techniques which have been developed and validated for measuring different aspects of lipid metabolism and some of the limitations of the methodology.
authors.affiliation: Diabetes and Metabolic MedicineFaculty of Health and Metabolic Sciences, University of Surrey, Leggett Building, Daphne Jackson Road, Manor Park, Guildford GU2 7WG, UK m.umpleby@surrey.ac.uk.
authors.forename: A Margot
authors.initials: AM
authors.lastname: Umpleby
mesh_terms: D006728:Hormones / Q000032:analysis*; D006801:Humans; D007554:Isotopes; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*
chemical_list: D006728:Hormones; D007554:Isotopes; D008055:Lipids
keywords: cholesterol; fatty acids; lipoproteins; stable isotopes; tracers