title: Inhibition of Aurora A promotes chemosensitivity via inducing cell cycle arrest and apoptosis in cervical cancer cells.
abstract: Aurora kinase A (AurA) regulates genomic instability and tumorigenesis in multiple cancer types. Although some studies have reported that Aur A may predict cervical cancer outcomes, its precise function and molecular mechanism in cervical cancer pathogenesis remain unclear. In this study, by overexpression or silencing of Aur A in cervical cancer cell lines, we found that overexpression of Aur A promoted cell proliferation through G1/S cell cycle transition and anti-apoptosis, xenograft tumor growth and chemoresistance to Taxol. We further found that inhibition of Aur A with its specific inhibitor VX-680 enhanced the antitumor effect of Taxol via inducing apoptosis. Moreover, the clinical analysis from tissue samples demonstrated that Aur A was overexpressed, and the expression of Aur A and pERK1/2 was negatively correlated in cervical cancer tissues. The above results may provide some potential insights in treatment of cervical cancer in clinic.
authors.affiliation: Cancer Institute, Fudan University Shanghai Cancer Center Shanghai 200032, China ; Department of Gynecological Oncology, Fudan University Shanghai Cancer Center Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University Shanghai 200032, China ; Department of Obstetrics and Gynecology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine Shanghai 200062, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Sun
authors.affiliation: Cancer Institute, Fudan University Shanghai Cancer Center Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University Shanghai 200032, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Yang
authors.affiliation: Department of Breast Oncology, Fudan University Shanghai Cancer Center Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University Shanghai 200032, China.
authors.forename: Han
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University Shanghai 200032, China ; Department of Pathology, Fudan University Shanghai Cancer Center Shanghai 200032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chang
authors.affiliation: Department of Gynecological Oncology, Fudan University Shanghai Cancer Center Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University Shanghai 200032, China.
authors.forename: Hua-Ying
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Cancer Institute, Fudan University Shanghai Cancer Center Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University Shanghai 200032, China ; Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University Shanghai 200240, China.
authors.forename: Gong
authors.initials: G
authors.lastname: Yang
keywords: Aurora A; Taxol; VX-680; cervical cancer; chemoresistance