title: X-ray structure of cyanide-bound bovine heart cytochrome c oxidase in the fully oxidized state at 2.0 Å resolution.
abstract: The X-ray structure of cyanide-bound bovine heart cytochrome c oxidase in the fully oxidized state was determined at 2.0 Å resolution. The structure reveals that the peroxide that bridges the two metals in the fully oxidized state is replaced by a cyanide ion bound in a nearly symmetric end-on fashion without significantly changing the protein conformation outside the two metal sites.
authors.affiliation: Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Tokai, Ibaraki, Japan.
authors.forename: Naomine
authors.initials: N
authors.lastname: Yano
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Muramoto
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shinzawa-Itoh
authors.affiliation: Institute for Protein Research, Osaka University, Suita, Japan.
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamashita
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yoshikawa
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000302:isolation & purification; D008929:Mitochondria, Heart / Q000737:chemistry* / Q000201:enzymology; D008958:Models, Molecular; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D003576:Electron Transport Complex IV; D011190:Potassium Cyanide
keywords: cytochrome c oxidase; membrane protein