title: Arbuscular mycorrhizal colonization in field-collected terrestrial cordate gametophytes of pre-polypod leptosporangiate ferns (Osmundaceae, Gleicheniaceae, Plagiogyriaceae, Cyatheaceae).
abstract: To determine the mycorrhizal status of pteridophyte gametophytes in diverse taxa, the mycorrhizal colonization of wild gametophytes was investigated in terrestrial cordate gametophytes of pre-polypod leptosporangiate ferns, i.e., one species of Osmundaceae (Osmunda banksiifolia), two species of Gleicheniaceae (Diplopterygium glaucum, Dicranopteris linearis), and four species of Cyatheales including tree ferns (Plagiogyriaceae: Plagiogyria japonica, Plagiogyria euphlebia; Cyatheaceae: Cyathea podophylla, Cyathea lepifera). Microscopic observations revealed that 58 to 97% of gametophytes in all species were colonized with arbuscular mycorrhizal (AM) fungi. Fungal colonization was limited to the multilayered midrib (cushion) tissue in all gametophytes examined. Molecular identification using fungal SSU rDNA sequences indicated that the AM fungi in gametophytes primarily belonged to the Glomeraceae, but also included the Claroideoglomeraceae, Gigasporaceae, Acaulosporaceae, and Archaeosporales. This study provides the first evidence for AM fungal colonization of wild gametophytes in the Plagiogyriaceae and Cyatheaceae. Taxonomically divergent photosynthetic gametophytes are similarly colonized by AM fungi, suggesting that mycorrhizal associations with AM fungi could widely occur in terrestrial pteridophyte gametophytes.
authors.affiliation: Faculty of Agriculture, Saga University, 1 Honjyo-machi, Saga, 840-8752, Japan. ytsujita@cc.saga-u.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogura-Tsujita
authors.affiliation: Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Tokyo, 112-8681, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Hirayama
authors.affiliation: Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Tokyo, 112-8681, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Sakoda
authors.affiliation: Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Tokyo, 112-8681, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki, 305-0005, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ebihara
authors.affiliation: Mie Prefectural Museum, Isshinden-kouzubeta, Tsu, Mie, 514-0061, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Morita
authors.affiliation: Department of Chemical and Biological Sciences, Japan Women's University, 2-8-1 Mejirodai, Tokyo, 112-8681, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Imaichi
mesh_terms: D058448:Biota; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D029624:Ferns / Q000382:microbiology*; D008853:Microscopy; D038821:Mycorrhizae / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D018744:DNA, Plant; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: Arbuscular mycorrhizal fungi; Cyatheales; Gametophyte; Gleicheniales; Osmundales; Pteridophyte