title: Establishment and Use of Mouse Haploid ES Cells.
abstract: Haploid genetics has facilitated new insights into mammalian pathways and disease mechanisms. Most animal cells are diploid, and mammalian haploid cell cultures have remained elusive for a long time. Recent methodological progress has enabled the routine derivation of haploid stem cell lines from mammalian haploid embryos. Here we provide detailed protocols for the establishment, culture, and manipulation of parthenogenetic and androgenetic haploid embryonic stem cells from mouse embryos.
authors.affiliation: Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Martin
authors.initials: M
authors.lastname: Leeb
authors.affiliation: Laboratory of Mammalian Molecular Embryology, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom.
authors.forename: Anthony C F
authors.initials: ACF
authors.lastname: Perry
authors.affiliation: Institute of Molecular Health Sciences, Department of Biology, Swiss Federal Institute of Technology, ETH Zürich, Zürich, Switzerland.
authors.forename: Anton
authors.initials: A
authors.lastname: Wutz
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D005821:Genetic Techniques; D006238:Haploidy; D051379:Mice / Q000196:embryology* / Q000235:genetics; D016296:Mutagenesis; D009865:Oocytes / Q000737:chemistry / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods
keywords: androgenotes; embryonic stem cells; haploid ES cells; oocyte; parthenogenotes