title: The relationship between antioxidant enzymes and lipid peroxidation in senescent rat erythrocytes.
abstract: The aim of this study was to gain more complete information about the relationships between some endogenous antioxidants and the malondialdehyde (MDA) as a marker of lipid peroxidation, during D-galactose induced senescence. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and the concentrations of uric acid (UA) in plasma and MDA in erythrocyte's hemolysate, were determined in 15 D-galactose (D-gal), treated rats and compared with 15 placebo. The activity of the erythrocyte's CAT was found significantly increased due to the senescence. The ratio of the activities of antioxidant enzymes R=SOD/(GPx+CAT) was significantly decreased due to the senescence and negatively correlated with the MDA (rho=-0.524, p=0.045). The antioxidant enzymes SOD and GPx negatively correlated with the MDA, while CAT displayed no correlation. Further, the UA positively correlated with the ratio of activities of the antioxidant enzymes R=SOD/(GPx+CAT), (rho=0.564, p=0.029 for senescent rats). Obtained results may contribute to better understanding of the process of D-gal induced senescence in the erythrocytes.
authors.affiliation: Faculty of Natural Sciences and Mathematics, Institute of Biology, "Sts, Cyril and Methodius" University, Skopje, Macedonia. mitkom@pmf.ukim.mk.
authors.forename: M
authors.initials: M
authors.lastname: Mladenov
authors.forename: M
authors.initials: M
authors.lastname: Gokik
authors.forename: N
authors.initials: N
authors.lastname: Hadzi-Petrushev
authors.forename: I
authors.initials: I
authors.lastname: Gjorgoski
authors.forename: N
authors.initials: N
authors.lastname: Jankulovski
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D016922:Cellular Senescence; D004912:Erythrocytes / Q000201:enzymology*; D005690:Galactose; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014527:Uric Acid; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005690:Galactose