title: Transcriptomic Analysis Reveals Possible Influences of ABA on Secondary Metabolism of Pigments, Flavonoids and Antioxidants in Tomato Fruit during Ripening.
abstract: Abscisic acid (ABA) has been proven to be involved in the regulation of climacteric fruit ripening, but a comprehensive investigation of its influence on ripening related processes is still lacking. By applying the next generation sequencing technology, we conducted a comparative analysis of the effects of exogenous ABA and NDGA (Nordihydroguaiaretic acid, an inhibitor of ABA biosynthesis) on tomato fruit ripening. The high throughput sequencing results showed that out of the 25728 genes expressed across all three samples, 10388 were identified as significantly differently expressed genes. Exogenous ABA was found to enhance the transcription of genes involved in pigments metabolism, including carotenoids biosynthesis and chlorophyll degradation, whereas NDGA treatment inhibited these processes. The results also revealed the crucial role of ABA in flavonoids synthesis and regulation of antioxidant system. Intriguingly, we also found that an inhibition of endogenous ABA significantly enhanced the transcriptional abundance of genes involved in photosynthesis. Our results highlighted the significance of ABA in regulating tomato ripening, which provided insight into the regulatory mechanism of fruit maturation and senescence process.
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Wangshu
authors.initials: W
authors.lastname: Mou
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Luo
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Linchun
authors.initials: L
authors.lastname: Mao
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Tiejin
authors.initials: T
authors.lastname: Ying
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D016000:Cluster Analysis; D019295:Computational Biology; D005419:Flavonoids / Q000096:biosynthesis / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010860:Pigments, Biological / Q000378:metabolism*; D064210:Secondary Metabolism / Q000187:drug effects* / Q000235:genetics*; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010860:Pigments, Biological; D000040:Abscisic Acid