title: Cytochrome cbb3 of Thioalkalivibrio is a Na+-pumping cytochrome oxidase.
abstract: Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb3 cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb3 cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ion-pumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia; muntyan@genebee.msu.ru skulach@genebee.msu.ru.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Muntyan
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia;
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Cherepanov
authors.affiliation: Department of Biochemistry, University of Turku, 20014 Turku, Finland;
authors.forename: Anssi M
authors.initials: AM
authors.lastname: Malinen
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia; Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland;
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Bloch
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia; Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands;
authors.forename: Dimitry Y
authors.initials: DY
authors.lastname: Sorokin
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia;
authors.forename: Inna I
authors.initials: II
authors.lastname: Severina
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia;
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Ivashina
authors.affiliation: Department of Biochemistry, University of Turku, 20014 Turku, Finland;
authors.forename: Reijo
authors.initials: R
authors.lastname: Lahti
authors.affiliation: Microbial Systems Ecology, Department of Aquatic Microbiology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.
authors.forename: Gerard
authors.initials: G
authors.lastname: Muyzer
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia; muntyan@genebee.msu.ru skulach@genebee.msu.ru.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Skulachev
mesh_terms: D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D020560:Proteobacteria / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: C414443:cbb3 oxidase; D003576:Electron Transport Complex IV; D000254:Sodium-Potassium-Exchanging ATPase
keywords: alkaliphily; cbb3-type oxidase; cytochrome c oxidase; sodium pumping