title: [Selective enrichment of Pseudomonas spp. in the rhizoplane of different plant species].
abstract: In contrast to rhizobia-legume symbiosis, the specificity for root colonization by pseudomonads seems to be less strict. However, several studies about bacterial diversity in the rhizosphere highlight the influence of plant species on the selective enrichment of certain microorganisms from the bulk soil community. In order to evaluate the effect that different crops have on the structure of pseudomonad community on the root surface, we performed plant trap experiments, using surface-disinfected maize, wheat or soybean seeds that were sown in pots containing the same pristine soil as substrate. Rhizoplane suspensions were plated on a selective medium for Pseudomonas, and pooled colonies served as DNA source to carry out PCR-RFLP community structure analysis of the pseudomonads-specific marker genes oprF and gacA. PCR-RFLP profiles were grouped by plant species, and were distinguished from those of bulk soil samples. Partial sequencing of 16S rDNA genes of some representative colonies of Pseudomonas confirmed the selective enrichment of distinctive genotypes in the rhizoplane of each plant species. These results support the idea that the root systems of agricultural crops such as soybean, maize and wheat, select differential sets of pseudomonads from the native microbial repertoire inhabiting the bulk soil.
authors.affiliation: Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Marrero
authors.affiliation: Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
authors.forename: Betina
authors.initials: B
authors.lastname: Agaras
authors.affiliation: Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Wall
authors.affiliation: Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina. Electronic address: cvalver@unq.edu.ar.
authors.forename: Claudio
authors.initials: C
authors.lastname: Valverde
mesh_terms: D001118:Argentina; D044822:Biodiversity; D005798:Genes, Bacterial; D005838:Genotype; D018517:Plant Roots / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification*; D012639:Seeds / Q000382:microbiology; D012641:Selection, Genetic; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology; D013045:Species Specificity; D014908:Triticum / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology
keywords: Plant growth-promoting rhizobacteria; Pseudomonas; Rhizoplane, Differential colonization; Rizobacterias promotoras del crecimiento vegetal; Rizoplano, Colonización diferencial