title: Influence of Nano-HA Coated Bone Collagen to Acrylic (Polymethylmethacrylate) Bone Cement on Mechanical Properties and Bioactivity.
abstract: OBJECTIVE
  This research investigated the mechanical properties and bioactivity of polymethylmethacrylate (PMMA) bone cement after addition of the nano-hydroxyapatite(HA) coated bone collagen (mineralized collagen, MC).
  
  
  METHODS
  The MC in different proportions were added to the PMMA bone cement to detect the compressive strength, compression modulus, coagulation properties and biosafety. The MC-PMMA was embedded into rabbits and co-cultured with MG 63 cells to exam bone tissue compatibility and gene expression of osteogenesis.
  
  
  RESULTS
  15.0%(wt) impregnated MC-PMMA significantly lowered compressive modulus while little affected compressive strength and solidification. MC-PMMA bone cement was biologically safe and indicated excellent bone tissue compatibility. The bone-cement interface crosslinking was significantly higher in MC-PMMA than control after 6 months implantation in the femur of rabbits. The genes of osteogenesis exhibited significantly higher expression level in MC-PMMA.
  
  
  CONCLUSIONS
  MC-PMMA presented perfect mechanical properties, good biosafety and excellent biocompatibility with bone tissues, which has profoundly clinical values.
authors.affiliation: Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, 100730, PR China.
authors.forename: Xisheng
authors.initials: X
authors.lastname: Weng
authors.affiliation: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, 100730, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Bian
authors.affiliation: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, 100730, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing, 100087, PR China.
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing, 100087, PR China.
authors.forename: Zhiye
authors.initials: Z
authors.lastname: Qiu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D015415:Biomarkers / Q000378:metabolism; D001696:Biomechanical Phenomena; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D019245:Compressive Strength; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005269:Femur / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D005347:Fibroblasts; D015870:Gene Expression; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D001843:Bone Cements; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D015675:Osteocalcin; D003094:Collagen; D019904:Polymethyl Methacrylate; D017886:Durapatite