title: LC-MS/MS for the simultaneous determination of polar endogenous ADMA and CML in plasma and urine from diabetics.
abstract: BACKGROUND
  Asymmetric dimethylarginine (ADMA) and ε-N-carboxymethyl-L-lysine (CML) are microvascular risk factors and potential biomarkers of diabetic microvascular complication.
  
  
  RESULTS
  Sample preparation was achieved using acetonitrile for protein precipitation step. ADMA, CML and IS CML-d2 were separated with gradient on a Welch Ultimate(®) XB- NH2 column. The assays were validated according to current bioanalytical guidelines with respect to specificity, linearity (20-1000 ng/ml for ADMA in human plasma, 50-2000 ng/ml in urine, 10-500 ng/ml for CML in human plasma and urine), accuracy and precision, extraction recovery, matrix effect and stability.
  
  
  CONCLUSIONS
  The LC-MS/MS method was successfully applied to quantification of ADMA and CML in plasma and urine samples from healthy individuals and patients with diabetic nephropathy.
authors.affiliation: 1Department of Physical Health, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Jing
authors.forename: Liqing
authors.initials: L
authors.lastname: Kuang
authors.forename: Naifeng
authors.initials: N
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000652:urine; D003928:Diabetic Nephropathies / Q000097:blood / Q000652:urine; D006801:Humans; D016014:Linear Models; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D012189:Retrospective Studies; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C018524:N,N-dimethylarginine; C048496:N(6)-carboxymethyllysine; D001120:Arginine; D008239:Lysine