title: Confined chemiluminescence detection of nanomolar levels of H2O2 in a paper-plastic disposable microfluidic device using a smartphone.
abstract: We report the design and characterization of a disposable light shielded paper-plastic microfluidic device that can detect nanomolar levels of H2O2 using a smartphone camera and a light sealed accessory. Chemiluminescence reaction of H2O2 with bis(2,4,6-trichlorophenyl)oxalate in the presence of rubrene and imidazole is confined to a paper reaction site where the individual reagents are delivered via plastic microfluidic channels. The net photon emission from the chemiluminescence reactions, detected by using a smartphone, is correlated with H2O2 concentrations. With a total reagent volume of 25 μL, the sensor system was able to detect H2O2 concentrations as low as 250 nM. The smartphone based chemiluminescence sensing system has great potential as a point of care diagnostic tool for monitoring nanomolar levels of H2O2 in biological samples.
authors.affiliation: Department of Mechanical Engineering, Lyle School of Engineering, Southern Methodist University, 3101 Dyer Street, Dallas, TX 75205, USA. rennyedwin@gmail.com.
authors.forename: Elise
authors.initials: E
authors.lastname: Lebiga
authors.forename: Renny
authors.initials: R
authors.lastname: Edwin Fernandez
authors.forename: Ali
authors.initials: A
authors.lastname: Beskok