title: Formation of Silver Nanostructures by Rolling Circle Amplification Using Boranephosphonate-Modified Nucleotides.
abstract: We investigate the efficiency of incorporation of boranephosphonate-modified nucleotides by phi29 DNA polymerase and present a simple method for forming large defined silver nanostructures by rolling circle amplification (RCA) using boranephosphonate internucleotide linkages. RCA is a linear DNA amplification technique that can use specifically circularized DNA probes for detection of target nucleic acids and proteins. The resulting product is a collapsed single-stranded DNA molecule with tandem repeats of the DNA probe. By substituting each of the natural nucleotides with the corresponding 5'-(α-P-borano)deoxynucleosidetriphosphate, only a small reduction in amplification rate is observed. Also, by substituting all four natural nucleotides, it is possible to enzymatically synthesize a micrometer-sized, single-stranded DNA molecule with only boranephosphonate internucleotide linkages. Well-defined silver particles are then readily formed along the rolling circle product.
authors.affiliation: †Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SE-751 85, Sweden.
authors.forename: Camilla
authors.initials: C
authors.lastname: Russell
authors.affiliation: ‡Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, United States.
authors.forename: Subhadeep
authors.initials: S
authors.lastname: Roy
authors.affiliation: ‡Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, United States.
authors.forename: Saheli
authors.initials: S
authors.lastname: Ganguly
authors.affiliation: §Science for Life laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-171 21, Sweden.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qian
authors.affiliation: ‡Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, United States.
authors.forename: Marvin H
authors.initials: MH
authors.lastname: Caruthers
authors.affiliation: †Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SE-751 85, Sweden.|§Science for Life laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-171 21, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
mesh_terms: D001880:Boranes / Q000737:chemistry*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D009711:Nucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D001880:Boranes; D009711:Nucleotides; D010710:Phosphates; C510710:boranophosphate; D012834:Silver