title: Novel Soluble Dietary Fiber-Tannin Self-Assembled Film: A Promising Protein Protective Material.
abstract: In this experiment, a natural promising protein protective film was fabricated through soluble dietary fiber (SDF)-tannin nanocluster self-assembly. FT-IR, XRD, and DSC tests were employed to investigate the interaction between the SDF and tannins before and after cross-linking induced by calcium ion. On the other hand, referring to the SEM and TEM results, the self-assembly process of the protein protective film could be indicated as follows: first, calcium ion, with its cross-ability, served as the "nucleus"; SDF and tannins were combined to prepare the nanoscale SDF-tannin clusters; then, the clusters were homogeneously deposited on the surface of protein to form a protective film by self-assembling hydrogen bond between tannin component of clusters as "adhesive" and protein in aqueous solutions under very mild conditions. Film thickness could also be controlled by tannin of different concentrations ranging from 114 to 1384 μm. Antibacterial test and in vitro cytotoxicity test proved that the film had a broad spectrum of antimicrobial properties and excellent cell biocompatibility, respectively, which might open up new applications in the food preservation and biomedical fields.
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Song
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Shuang-shuang
authors.initials: SS
authors.lastname: Ge
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D004043:Dietary Fiber / Q000032:analysis* / Q000494:pharmacology; D005519:Food Preservation; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013634:Tannins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004043:Dietary Fiber; D011506:Proteins; D013634:Tannins
keywords: nanoclusters; protective film; self-assembly; soluble dietary fiber; tannin