title: Immunofluorescence vs immunochemiluminescent methods: AIA 2000 vs Immulite 2000.
abstract: The aim of this study was to evaluate the measurement of C-peptide, insulin, sex hormone binding globulin (SHBG), and dehydroepiandrosterone sulfate (DHEAS), tests of specific clinical value that are not frequently measured in routine clinical laboratories, carried out using an automated system, AIA 2000 (Tosoh). The obtained data demonstrated that the evaluated system, characterized by fluorescence system detector provides satisfactory analytical performance comparable to those of different instrument that use the well known and widely diffused chemiluminescent principle (Immulite 2000). These characteristics allow an excellent comparability between methods for the measurement of same specific hormones not frequently used in clinical practice.
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padua, Italy. Electronic address: chiara.cosma@sanita.padova.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Cosma
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padua, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Baggio
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padua, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Casarotti
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padua, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Zaninotto
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padua, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Faggian
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padua, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Plebani
mesh_terms: D002096:C-Peptide / Q000378:metabolism; D019411:Clinical Laboratory Techniques / Q000379:methods*; D019314:Dehydroepiandrosterone Sulfate / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism
chemical_list: D002096:C-Peptide; D007328:Insulin; D012738:Sex Hormone-Binding Globulin; D019314:Dehydroepiandrosterone Sulfate
keywords: C-peptide; DHEA-S; Insulin; SHBG