title: Quantitative proteomic analysis of single or fractionated radiation-induced proteins in human breast cancer MDA-MB-231 cells.
abstract: BACKGROUND
  Radiotherapy is widely used to treat cancer alone or in combination with surgery, chemotherapy, and immunotherapy. However, damage to normal tissues and radioresistance of tumor cells are major obstacles to successful radiotherapy. Furthermore, the immune network around tumors appears to be connected to tumor progression and recurrence.
  
  
  METHODS
  We investigated the cytosolic proteins produced by irradiated tumor cells by using a quantitative proteomic approach based on stable isotope labeling by amino acids in cell culture. MDA-MB-231 breast cancer cells were treated with a single or fractionated 10 Gray dose of (137)Cs γ-radiation, which was selected based on cell viability.
  
  
  RESULTS
  Radiation-induced proteins were differentially expressed based on the fractionated times of radiation and were involved in multiple biological functions, including energy metabolism and cytoskeleton organization. We identified 46 proteins increased by at least 1.3-fold, and high ranks were determined for cathepsin D, gelsolin, arginino-succinate synthase 1, peroxiredoxin 5, and C-type mannose receptor 2.
  
  
  CONCLUSIONS
  These results suggest that a number of tumor-derived factors upregulated by γ-radiation are promising targets for modulation of the immune response during radiation treatment.
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706 Korea ; Laboratory of Immunology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Mi-Hyoung
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706 Korea.
authors.forename: Seung-Youn
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706 Korea.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706 Korea.
authors.forename: Sang-Gu
authors.initials: SG
authors.lastname: Hwang
authors.affiliation: Laboratory of Immunology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Woo
authors.affiliation: Department of Microbiological Engineering, Kon-Kuk University, Seoul, Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.affiliation: Radiation Health Institute, Korea Hydro & Nuclear Power Co., Ltd, Seoul, Korea.
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Nam
authors.affiliation: Department of Applied Bioscience, CHA University, Gyeonggi-do, Korea.
authors.forename: Dae-Seog
authors.initials: DS
authors.lastname: Lim
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706 Korea.
authors.forename: Jie-Young
authors.initials: JY
authors.lastname: Song
keywords: Breast cancer; Proteomics; Radiation therapy; SILAC