title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of NAD synthetase from methicillin-resistant Staphylococcus aureus.
abstract: Staphylococcus aureus is an important human and animal pathogen that causes a wide range of infections. The prevalence of multidrug-resistant S. aureus strains in both hospital and community settings makes it imperative to characterize new drug targets to combat S. aureus infections. In this context, enzymes involved in NAD metabolism and synthesis are significant drug targets as NAD is a central player in several cellular processes. NAD synthetase catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide, making it a crucial intermediate enzyme linked to the biosynthesis of several amino acids, purine and pyrimidine nucleotides, coenzymes and antibiotics.
authors.affiliation: Department of Biosciences and Technology, Defence Institute of Advanced Technology, Pune, Maharashtra 411 025, India.
authors.forename: Gajanan Kashinathrao
authors.initials: GK
authors.lastname: Arbade
authors.affiliation: Department of Biosciences and Technology, Defence Institute of Advanced Technology, Pune, Maharashtra 411 025, India.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Srivastava
mesh_terms: D019732:Amide Synthases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D053858:Metabolic Networks and Pathways; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry* / Q000201:enzymology; D008969:Molecular Sequence Data; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D009243:NAD; D019732:Amide Synthases; C038479:NAD+ synthase
keywords: NAD synthetase; Staphylococcus aureus