title: The Chromatin Remodeling Protein Bptf Promotes Posterior Neuroectodermal Fate by Enhancing Smad2-Activated wnt8a Expression.
abstract: During vertebrate embryogenesis, the neuroectoderm is induced from dorsal ectoderm and then partitioned into anterior and posterior neuroectodermal domains by posteriorizing signals, such as Wnt and fibroblast growth factor. However, little is known about epigenetic regulation of posteriorizing gene expression. Here, we report a requirement of the chromatin remodeling protein Bptf for neuroectodermal posteriorization in zebrafish embryos. Knockdown of bptf leads to an expansion of the anterior neuroectoderm at the expense of the posterior ectoderm. Bptf functionally and physically interacts with p-Smad2, which is activated by non-Nodal TGF-β signaling, to promote the expression of wnt8a, a critical gene for neural posteriorization. Bptf and Smad2 directly bind to and activate the wnt8a promoter through recruiting NURF remodeling complex. When bptf function or TGF-β signal transduction is inhibited, the nucleosome density on the wnt8a promoter is increased. We propose that Bptf and TGF-β/Smad2 mediate nucleosome remodeling to regulate wnt8a expression and hence neural posteriorization.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and.
authors.forename: Zhaoting
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and.
authors.forename: Shi
authors.initials: S
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and.
authors.forename: Hanqiao
authors.initials: H
authors.lastname: Shang
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China qiangwang@ioz.ac.cn mengam@mail.tsinghua.edu.cn.
authors.forename: Anming
authors.initials: A
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, and qiangwang@ioz.ac.cn mengam@mail.tsinghua.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism*; D001549:Benzamides; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004149:Dioxoles; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D047468:Immunoprecipitation; D008297:Male; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D054258:Neural Plate / Q000196:embryology* / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D051899:Smad2 Protein / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*; D000092468:Bromodomain Containing Proteins
chemical_list: D034961:Antigens, Nuclear; D001549:Benzamides; D003598:Cytoskeletal Proteins; D004149:Dioxoles; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D051899:Smad2 Protein; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D051153:Wnt Proteins; D029961:Zebrafish Proteins; C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; C406238:fetal Alzheimer antigen; D000092468:Bromodomain Containing Proteins; C110484:tdgf1 protein, zebrafish; C571228:tp53 protein, zebrafish; C094521:wnt8a protein, zebrafish
keywords: bptf; neural posteriorization; nucleosome remodeling; smad2; wnt8a