title: Exploring the Material-Induced Transcriptional Landscape of Osteoblasts on Bone Graft Materials.
abstract: During the past decades, there have been major advances in the field of biomaterials, thereby generating a vast variety of materials for a broad range of tissue engineering and regeneration applications. Although gene expression profiling has been used occasionally in biomaterial research, its usefulness for understanding cell-biomaterial interactions should be further explored for it to fulfill its promise as a tool to assess and improve material properties. Here, the transcriptional landscape induced by 23 materials is explored with a variety of properties within the scope of bone regeneration. An osteoblast cell line is used to identify the gene expression profiles that can be adopted in response to biophysical and chemical cues. It is shown that TGF-β and WNT signaling may be involved in the cellular response to osteoinductive materials along with differential cell adhesion kinetics via attenuated FAK signaling. The previously reported effect of calcium and phosphate on BMP2 and TGF-β signaling is confirmed and the biological effect of the addition of nanohydroxyapatite in poly (d,l-lactic acid) polymer particles is studied. Together with future applications, this approach will help researchers understand cellular responses in relation to material properties, which will promote the development of more effective biomaterials for applications in tissue regeneration.
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Groen
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.
authors.forename: Niloofar
authors.initials: N
authors.lastname: Tahmasebi
authors.affiliation: Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Yamaguchi, 755-8505, Japan.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Shimizu
authors.affiliation: Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Yamaguchi, 755-8505, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Sano
authors.affiliation: Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Yamaguchi, 755-8505, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanda
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.|Xpand Biotechnology BV, Prof. Bronkhorstlaan 10-D, 3723, MB, Bilthoven, The Netherlands.
authors.forename: Davide
authors.initials: D
authors.lastname: Barbieri
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.|Xpand Biotechnology BV, Prof. Bronkhorstlaan 10-D, 3723, MB, Bilthoven, The Netherlands.
authors.forename: Huipin
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6200 MD, Maastricht, The Netherlands.
authors.forename: Pamela
authors.initials: P
authors.lastname: Habibovic
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6200 MD, Maastricht, The Netherlands.
authors.forename: Clemens A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, 7500, AE, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6200 MD, Maastricht, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D025341:Principal Component Analysis; D015398:Signal Transduction / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D059467:Transcriptome / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite
keywords: bone regeneration; cell-biomaterial interactions; transcriptional profiling