title: [miR-141-3p regulates the expression of androgen receptor by targeting its 3'UTR in prostate cancer LNCaP cells].
abstract: OBJECTIVE
  To detect the target degradation of miR-141-3p on androgen receptor (AR) gene in LNCaP cells and demonstrate whether AR gene is a target of miR-141-3p.
  
  
  METHODS
  After prostate cancer cell line LNCaP was transfected with miR-141-3p mimics, expression levels of AR mRNA and protein in the LNCaP cells were detected by reverse transcription PCR and Western blotting, respectively. The 3'untranslated regions (3'UTR) of AR mRNA containing the binding site of miR-141-3p was amplified by PCR and inserted into pmiR-report vector (a 3'downstream luciferase reporter gene). The product was termed pmiR-AR-3'UTR. Double luciferase reporter system was employed to verify the potential target effect of miR-141-3p on pmiR-AR-3'UTR.
  
  
  RESULTS
  Transfection of miR-141-3p mimics decreased both mRNA and protein expression levels of AR in LNCaP cells. Compared with control group, miR-141-3p transfection significantly inhibited the activity of luciferase of pmiR-AR-3'UTR.
  
  
  CONCLUSIONS
  AR is a direct target gene of miR-141-3p.
authors.affiliation: Central Laboratory, Tangdu hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Central Laboratory, Tangdu hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Yongri
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Central Laboratory, Tangdu hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Man
authors.initials: M
authors.lastname: Lu
authors.affiliation: Central Laboratory, Tangdu hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Wei
authors.affiliation: Central Laboratory, Tangdu hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Huizhong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000473:pathology*; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C410798:AR protein, human; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D011944:Receptors, Androgen