title: [Effect of reduced oxygen concentrations and hydrogen sulfide on the amino acid metabolism and mesenchymal cells proliferation].
abstract: We investigated the effect of hydrogen sulfide donor (10(-12) mol/l NaHS--I group) alone and together with the reduced oxygen concentrations (5% O2--II group, 3% O2--III group, 24 h) on the biological processes of human stem cells culture. It was shown that the cells proliferation by the third day of cultivation in I, II and III group decreased 1,7; 2,8 and 4,2 times. On the 4th day of culture proliferation inhibited in I, II and III group by 29; 33 and 54% compared to the control. Thus, adverse effects NaHS enhanced by reducing the oxygen concentration. It was established that in all experimental versions rapidly absorbed from the culture medium amino acids: cysteine and cystine, serine and aspartic acid, valine and tryptophan, proline and hydroxyproline, which are involved in the synthesis of proteins, in particular collagen. In the culture medium increased the concentration of free amino acids of the three factions: arginine, histidine and taurine; glycine and methionine; alanine and glutamine. We believe that in the applied concentration of hydrogen sulfide donor in conditions of low oxygen in a gaseous medium incubation inhibits the proliferation and alters the amino acid metabolism of human cells line 4BL.
authors.forename: L N
authors.initials: LN
authors.lastname: Plotnikova
authors.forename: V A
authors.initials: VA
authors.lastname: Berezovskii
authors.forename: S P
authors.initials: SP
authors.lastname: Veselskii
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D015687:Cell Hypoxia; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D013440:Sulfides / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D013440:Sulfides; D003094:Collagen; C025451:sodium bisulfide; D010100:Oxygen; D006862:Hydrogen Sulfide