title: Phosphoproteome dynamics of Saccharomyces cerevisiae under heat shock and cold stress.
abstract: The ability of cells and organisms to survive and function through changes in temperature evolved from their specific adaptations to nonoptimal growth conditions. Responses to elevated temperatures have been studied in yeast and other model organisms using transcriptome profiling and provided valuable biological insights on molecular mechanisms involved in stress tolerance and adaptation to adverse environment. In contrast, little is known about rapid signaling events associated with changes in temperature. To gain a better understanding of global changes in protein phosphorylation in response to heat and cold, we developed a high temporal resolution phosphoproteomics protocol to study cell signaling in Saccharomyces cerevisiae. The method allowed for quantitative analysis of phosphodynamics on 2,777 phosphosites from 1,228 proteins. The correlation of kinetic profiles between kinases and their substrates provided a predictive tool to identify new putative substrates for kinases such as Cdc28 and PKA. Cell cycle analyses revealed that the increased phosphorylation of Cdc28 at its inhibitory site Y19 during heat shock is an adaptive response that delays cell cycle progression under stress conditions. The cellular responses to heat and cold were associated with extensive changes in phosphorylation on proteins implicated in transcription, protein folding and degradation, cell cycle regulation and morphogenesis.
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Kanshin
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada Department of Chemistry, Université de Montréal, Montréal, QC, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Kubiniok
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada Department of Pathology and Cell Biology, Université de Montréal, Montréal, QC, Canada.
authors.forename: Yogitha
authors.initials: Y
authors.lastname: Thattikota
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada Department of Pathology and Cell Biology, Université de Montréal, Montréal, QC, Canada.
authors.forename: Damien
authors.initials: D
authors.lastname: D'Amours
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada Department of Chemistry, Université de Montréal, Montréal, QC, Canada Department of Biochemistry, Université de Montréal, Montréal, QC, Canada pierre.thibault@umontreal.ca.
authors.forename: Pierre
authors.initials: P
authors.lastname: Thibault
mesh_terms: D003080:Cold Temperature; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response / Q000235:genetics*; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D010766:Phosphorylation / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D015398:Signal Transduction
chemical_list: D010750:Phosphoproteins
keywords: cold stress; dynamics; heat shock; phosphoproteomics; signaling