title: A Simple Evaluation Method for the Quality of Dietary Protein in Rats Using an Indicator Amino Acid Oxidation Technique.
abstract: We demonstrated that the indicator amino acid oxidation (IAAO) method could be employed for the evaluation of quality of dietary protein by comparing the protein intakes required to meet metabolic demand in rats fed different proteins. The objective of this study was to validate a simple evaluation method for determining the quality of dietary protein using the IAAO technique. Male Sprague-Dawley rats (5-6 wk old) were fed meals composed of graded protein, using either casein, wheat gluten (WG), soy protein isolate (SPI), or egg white protein (EW), every 3 h from 09:00 to 18:00. Administration of L-[1-(13)C]phenylalanine was performed hourly from 15:00 to 18:00. The (13)CO2 level in breath CO2 was measured at 18:30. The protein intake values required to meet the metabolic demand based on the breath (13)CO2 data for the dietary casein, WG, SPI, and EW intake were 18.0, 22.2, 17.5, and 10.1 g/kg BW/d, respectively. The breath (13)CO2 concentrations corresponding to the protein intake of 7.5 g/kg BW/d for casein, WG, SPI, and EW were 9.8, 10.9, 10.3, and 8.9 (‰)/100 g BW, respectively. A significant correlation was demonstrated between the protein intake required to meet the metabolic demands and the (13)CO2 concentration in the breath for a protein intake of 7.5 g/kg BW/d (r=0.967; p<0.05). These results demonstrated that the protein intake required to meet metabolic demand could be estimated and that the quality of the dietary protein could be evaluated using the (13)CO2 concentration in the breath with a protein intake of 7.5 g/kg BW/d.
authors.affiliation: Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University.
authors.forename: Aki
authors.initials: A
authors.lastname: Ogawa
authors.forename: Haruka
authors.initials: H
authors.lastname: Murayama
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Hayamizu
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Masashi
authors.initials: M
authors.lastname: Kuwahata
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kido
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001944:Breath Tests / Q000379:methods; D002245:Carbon Dioxide / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D004032:Diet / Q000592:standards*; D004044:Dietary Proteins / Q000378:metabolism / Q000592:standards*; D004527:Egg Proteins / Q000378:metabolism; D008297:Male; D009751:Nutritional Requirements; D010084:Oxidation-Reduction; D010649:Phenylalanine / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D000596:Amino Acids; D002364:Caseins; D004044:Dietary Proteins; D004527:Egg Proteins; D010940:Plant Proteins; D002245:Carbon Dioxide; D010649:Phenylalanine