title: Screen for soil fungi highly resistant to dichloroaniline uncovers mostly Fusarium species.
abstract: Arylamines are frequent pollutants in soils. Fungi have proven to be efficient in detoxifying these chemicals by acetylating them using arylamine N-acetyl transferase enzymes. Here, we selected from natural soils fungi highly resistant to 3,4-dichloroaniline (DCA). Fusarium species were the most frequently isolated species, especially Fusarium solani. The sequenced strain of F. solani contains five NAT genes, as did all the DCA-resistant isolates. RT-PCR analysis showed that the five genes were expressed in F. solani. Expression of the F. solani genes in Podospora anserina and analysis of acetylation directly in F. solani showed that only the NhNAT2B gene conferred significant resistance to DCA and that F. solani likely uses pathways different from acetylation to resist high doses of DCA, as observed previously for Trichoderma.
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain (IED), F-75205 Paris, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Chan Ho Tong
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Unit of Functional and Adaptive Biology (BFA), CNRS UMR 8251, F-75205 Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Dairou
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Unit of Functional and Adaptive Biology (BFA), CNRS UMR 8251, F-75205 Paris, France.
authors.forename: Linh-Chi
authors.initials: LC
authors.lastname: Bui
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain (IED), F-75205 Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Bouillon
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Unit of Functional and Adaptive Biology (BFA), CNRS UMR 8251, F-75205 Paris, France.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rodrigues-Lima
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Unit of Functional and Adaptive Biology (BFA), CNRS UMR 8251, F-75205 Paris, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Dupret
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain (IED), F-75205 Paris, France. Electronic address: philippe.silar@univ-paris-diderot.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Silar
mesh_terms: D000123:Acetyltransferases; D000595:Amino Acid Sequence; D000814:Aniline Compounds / Q000633:toxicity*; D005670:Fusarium / Q000201:enzymology / Q000302:isolation & purification*; D020869:Gene Expression Profiling; D008658:Inactivation, Metabolic; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D000814:Aniline Compounds; D012989:Soil Pollutants; C014464:3,4-dichloroaniline; D000123:Acetyltransferases
keywords: Arylamine toxicity; Fusarium; N-acetyl transferases; Soil fungi