title: Improvement of butanol production from a hardwood hemicelluloses hydrolysate by combined sugar concentration and phenols removal.
abstract: The feasibility of using hardwood hemicellulosic pre-hydrolysate recovered from a dissolving pulping process for Acetone-Butanol-Ethanol (ABE) fermentation has been investigated. Dilutions and detoxification methods based on flocculation and nanofiltration were tested to determine the inhibitory concentration of phenolic compounds and to evaluate the efficiency of inhibitors removal on fermentation. Flocculation carried out with ferric sulfate was the most effective method for removal of phenolics (56%) and acetic acid (80%). The impact on fermentation was significant, with an ABE production of 6.40 g/L and 4.25 g/L when using flocculation or combined nanofiltration/flocculation respectively, as compared to a non-significant production for the untreated hydrolysate. By decreasing the toxicity effect of inhibitors, this study reports for the first time that the use of these techniques is efficient to increase the inhibitory concentration threshold of phenols, from 0.3g/L in untreated hydrolysate, to 1.1g/L in flocculated and in nanofiltrated and flocculated hydrolysates.
authors.affiliation: Biocatalysis and Industrial Enzymes Group, Laboratory of Microbial Ecology and Technology, National Institute of Applied Sciences and Technology (INSAT) Tunis, University of Carthage, Tunisia; Research Unit on Energy Efficiency and Sustainable Development of the Forest Biorefinery, Department of Chemical Engineering, Polytechnique Montréal, Montréal, QC, Canada.
authors.forename: Fatma
authors.initials: F
authors.lastname: Mechmech
authors.affiliation: Centre National en Electrochimie et en Technologies Environnementales, Shawinigan, QC, Canada.
authors.forename: Hassan
authors.initials: H
authors.lastname: Chadjaa
authors.affiliation: Centre National en Electrochimie et en Technologies Environnementales, Shawinigan, QC, Canada.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Rahni
authors.affiliation: Research Unit on Energy Efficiency and Sustainable Development of the Forest Biorefinery, Department of Chemical Engineering, Polytechnique Montréal, Montréal, QC, Canada. Electronic address: mariya.marinova@polymtl.ca.
authors.forename: Mariya
authors.initials: M
authors.lastname: Marinova
authors.affiliation: Laboratory of Natural Substances, National Institute of Research and Physical and Chemical Analysis (INRAP), Biotechno pole Sidi Thabet, Ariana, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Ben Akacha
authors.affiliation: Biocatalysis and Industrial Enzymes Group, Laboratory of Microbial Ecology and Technology, National Institute of Applied Sciences and Technology (INSAT) Tunis, University of Carthage, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Gargouri
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000096:Acetone / Q000737:chemistry; D000440:Butanols / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005290:Ferric Compounds / Q000737:chemistry; D006868:Hydrolysis; D010636:Phenols / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D014934:Wood / Q000737:chemistry*
chemical_list: D000440:Butanols; D002241:Carbohydrates; D005290:Ferric Compounds; D010636:Phenols; D011134:Polysaccharides; D000096:Acetone; C024823:ferric sulfate; D000431:Ethanol; C007916:hemicellulose; D019342:Acetic Acid
keywords: Bio-butanol; Flocculation; Nanofiltration; Phenolics; Wood biomass hydrolysate