title: Bioactive formulations prepared from fruiting bodies and submerged culture mycelia of the Brazilian edible mushroom Pleurotus ostreatoroseus Singer.
abstract: Pleurotus ostreatoroseus is a Brazilian edible mushroom whose chemical characterization and bioactivity still remain underexplored. In this study, the hydrophilic and lipophilic compounds as well as the antioxidant, anti-inflammatory and antimicrobial activities of formulations (ethanol extracts) prepared with its fruiting bodies and submerged culture mycelia were compared. The bioactive formulations contain at least five free sugars, four organic acids, four phenolic compounds and two tocopherols. The fruiting body-based formulation revealed higher reducing power, DPPH scavenging activity, β-carotene bleaching inhibition and lipid peroxidation inhibition in brain homogenates than the mycelium-based preparation, as well as higher anti-inflammatory and antimicrobial activities. The absence of hepatotoxicity was confirmed in porcine liver primary cells. These functional responses can be related to the levels of bioactive components including phenolic acids, organic acids and tocopherols.
authors.affiliation: Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5301-855 Bragança, Portugal. iferreira@ipb.pt.
authors.forename: Rúbia Carvalho Gomes
authors.initials: RC
authors.lastname: Corrêa
authors.forename: Aloisio Henrique Pereira
authors.initials: AH
authors.lastname: de Souza
authors.forename: Ricardo C
authors.initials: RC
authors.lastname: Calhelha
authors.forename: Lillian
authors.initials: L
authors.lastname: Barros
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamoclija
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
authors.forename: Rosane Marina
authors.initials: RM
authors.lastname: Peralta
authors.forename: Adelar
authors.initials: A
authors.lastname: Bracht
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D001938:Brazil; D003470:Culture Media / Q000737:chemistry; D048690:Fruiting Bodies, Fungal / Q000254:growth & development*; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development; D010084:Oxidation-Reduction; D020076:Pleurotus / Q000737:chemistry / Q000254:growth & development*; D014675:Vegetables / Q000737:chemistry / Q000254:growth & development
chemical_list: D000975:Antioxidants; D003470:Culture Media