title: Metallic nanocrystallites-incorporated ordered mesoporous carbon as labels for a sensitive simultaneous multianalyte electrochemical immunoassay.
abstract: This work reports on a facile, novel multianalyte electrochemical immunoassay for simultaneous detection of a-fetoprotein (AFP) and human epidermal growth factor receptor type-2 (HER-2) using metal-containing nanomaterials confined in the ordered mesoporous carbon matrix (OMC-M) as labels. Well-dispersed uniform metallic nanocrystallites incorporated OMC materials were fabricated through a simple, economical, and green preparative strategy toward phenolic resol as a carbon source and metal nitrate as metal sources. The large amount of metallic nanocrystallites loading on the OMC nanomaterials, greatly amplified the detection signals, and the good biocompatibility of carbon nanotubes-chitosan retained excellent stability for the sandwich-type immunoassay. Under optimal experimental conditions, the proposed immunoassay exhibited high sensitivity and selectivity for the detection of analytes, providing a better linear response range from 0.001 to 150 ng/mL for AFP and for HER-2, with a lower limit of detectionof 0.6p g/mL and 0.35 pg/mL (S/N=3), respectively. The immunosensor exhibited convenience, low cost, rapidity, good specificity, acceptable stability and reproducibility. Moreover, satisfactory results were obtained for the determination of AFP and HER-2 in real human serum samples, indicating that the developed immunoassay has the potential to find application in clinical detection of AFP and HER-2 and other tumor markers as an alternative approach.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Yishan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China. Electronic address: wanglsh@scut.edu.cn.
authors.forename: Lishi
authors.initials: L
authors.lastname: Wang
mesh_terms: D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002244:Carbon; D048271:Chitosan; D003429:Cross Reactions; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles; D037742:Nanotubes, Carbon; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood / Q000276:immunology; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology
chemical_list: D014408:Biomarkers, Tumor; D002244:Carbon; D048271:Chitosan; D037742:Nanotubes, Carbon; D018719:Erb-b2 Receptor Tyrosine Kinases; D000509:alpha-Fetoproteins; C097353:AFP protein, human; C508053:ERBB2 protein, human
keywords: A-fetoprotein; Electrochemical immunosensor; Human epidermal growth factor receptor type-2; Metallic nanocrystallites; Multiwalled carbon nanotubes; Ordered mesoporous carbon