title: Simultaneous screening for 45 poisonous alkaloids in blood by LC-MS/MS.
abstract: OBJECTIVE
  To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for 45 poisonous alkaloids in blood.
  
  
  METHODS
  Identification was based on the compound's retention time and two precursor-to-production transitions. The method involved a liquid-liquid extraction (LLE) followed by LC-MS/MS with multiple-reaction monitoring (MRM). When 1 mL of blood was extracted with diethyl ether at pH = 9.2 with SKF525A as the internal standard, the target compounds were analyzed with LC-MS/MS in the positive ionization mode.
  
  
  RESULTS
  The target alkaloids had good linearity (r>0.995 1), both the intra-day precision and inter-day precision being less than 14.77%. The limits of detection ranged from 0.05 to 25 ng/mL in blood.
  
  
  CONCLUSIONS
  The method is selective and sensitive in detecting poisonous alkaloids with a total running time of 12 minutes; therefore it was successfully applied to some actual cases of suspected alkaloids poisoning.
authors.forename: Jin-Xiao
authors.initials: JX
authors.lastname: Zhai
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000470:Alkaloids / Q000097:blood / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005554:Forensic Medicine; D006801:Humans; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids