title: Full-genome sequence of a novel myovirus, GF-2, infecting Edwardsiella tarda: comparison with other Edwardsiella myoviral genomes.
abstract: Edwardsiellosis, which is caused by Edwardsiella tarda, a Gram-negative bacterium, is one of the most serious infectious diseases in both marine and freshwater fish farms worldwide. Previously, we reported the complete genome sequences of three E. tarda-lytic bacteriophages (two podoviruses and a myovirus), which were isolated from fish tissues and fish-rearing seawater. Further genomic information regarding E. tarda phages is important for understanding phage-host interactions as well as for applications of the phages for the control of disease. Here, we report the complete genome sequence of a novel E. tarda phage (GF-2) of myovirus morphology (family Myoviridae), isolated from tissue homogenates of a cultured Japanese flounder (Paralichthys olivaceus) that succumbed to edwardsiellosis in Japan. The size of the entire genome was 43,129 bp, with a GC content of 51.3 % and containing 82 open reading frames (ORFs). The GF-2 genome possesses lysogeny-related genes that have not been found in the reported Edwardsiella phage genomes. Comparative genomics of Edwardsiella myophages suggest that the C-terminal domains of the tail fiber proteins have relevance to their host specificity. Thus, GF-2 genome information provides a novel resource for our understanding of the molecular mechanisms involved in their host specificity and for detection of E. tarda in aquaculture environments.
authors.affiliation: Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa, 236-8648, Japan, yasuike@affrc.go.jp.
authors.forename: Motoshige
authors.initials: M
authors.lastname: Yasuike
authors.forename: Issei
authors.initials: I
authors.lastname: Nishiki
authors.forename: Yuki
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Yoji
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Atushi
authors.initials: A
authors.lastname: Fujiwara
authors.forename: Emi
authors.initials: E
authors.lastname: Sugaya
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kawato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagai
authors.forename: Takanori
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ototake
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakai
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D001483:Base Sequence; D020594:Edwardsiella / Q000821:virology*; D020609:Edwardsiella tarda / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins