title: Subnetwork-Specific Homeostatic Plasticity in Mouse Visual Cortex In Vivo.
abstract: Homeostatic regulation has been shown to restore cortical activity in vivo following sensory deprivation, but it is unclear whether this recovery is uniform across all cells or specific to a subset of the network. To address this issue, we used chronic calcium imaging in behaving adult mice to examine the activity of individual excitatory and inhibitory neurons in the same region of the layer 2/3 monocular visual cortex following enucleation. We found that only a fraction of excitatory neurons homeostatically recover activity after deprivation and inhibitory neurons show no recovery. Prior to deprivation, excitatory cells that did recover were more likely to have significantly correlated activity with other recovering excitatory neurons, thus forming a subnetwork of recovering neurons. These network level changes are accompanied by a reduction in synaptic inhibition onto all excitatory neurons, suggesting that both synaptic mechanisms and subnetwork activity are important for homeostatic recovery of activity after deprivation.
authors.affiliation: MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House 4(th) Floor, London SE1 1UL, UK; Department of Neuroscience, Physiology, and Pharmacology, University College London, 21 University Street, London WC1E 6DE, UK.
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Barnes
authors.affiliation: MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House 4(th) Floor, London SE1 1UL, UK; Department of Neuroscience, Physiology, and Pharmacology, University College London, 21 University Street, London WC1E 6DE, UK.
authors.forename: Rosanna P
authors.initials: RP
authors.lastname: Sammons
authors.affiliation: MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House 4(th) Floor, London SE1 1UL, UK; Department of Neuroscience, Physiology, and Pharmacology, University College London, 21 University Street, London WC1E 6DE, UK.
authors.forename: R Irene
authors.initials: RI
authors.lastname: Jacobsen
authors.affiliation: MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House 4(th) Floor, London SE1 1UL, UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mackie
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland.
authors.forename: Georg B
authors.initials: GB
authors.lastname: Keller
authors.affiliation: MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House 4(th) Floor, London SE1 1UL, UK; Department of Neuroscience, Physiology, and Pharmacology, University College London, 21 University Street, London WC1E 6DE, UK. Electronic address: t.keck@ucl.ac.uk.
authors.forename: Tara
authors.initials: T
authors.lastname: Keck
mesh_terms: D000818:Animals; D005260:Female; D006706:Homeostasis / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009415:Nerve Net / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D010775:Photic Stimulation / Q000379:methods; D012683:Sensory Deprivation / Q000502:physiology*; D014793:Visual Cortex / Q000502:physiology*; D014795:Visual Pathways / Q000502:physiology*