title: Two-colour fluorescent imaging in organisms using self-assembled nano-systems of upconverting nanoparticles and molecular switches.
abstract: The water-insolubility of a potentially versatile photoresponsive 'turn-on' fluorescence probe was overcome by incorporating it into a nano-assembly containing an upconverting nanoparticle wrapped in an amphiphilic polymer. The appeal of the nano-system is not only in the ability to turn "on" and "off" the fluorescence from the organic chromophore using UV and visible light, it is in the fact that the nanoparticle acts as a static probe because it emits red and green light when excited by near infrared light, which is not effected by UV and visible light. This dual-functioning emission behaviour was demonstrated in live organisms.
authors.affiliation: 4D LABS, Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6. nbranda@sfu.ca.
authors.forename: Tuoqi
authors.initials: T
authors.lastname: Wu
authors.forename: Bob
authors.initials: B
authors.lastname: Johnsen
authors.forename: Zhaozhao
authors.initials: Z
authors.lastname: Qin
authors.forename: Masakazu
authors.initials: M
authors.lastname: Morimoto
authors.forename: David
authors.initials: D
authors.lastname: Baillie
authors.forename: Masahiro
authors.initials: M
authors.lastname: Irie
authors.forename: Neil R
authors.initials: NR
authors.lastname: Branda
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes