title: Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity.
abstract: MicroDNAs are <400-base extrachromosomal circles found in mammalian cells. Tens of thousands of microDNAs have been found in all tissue types, including sperm. MicroDNAs arise preferentially from areas with high gene density, GC content, and exon density from promoters with activating chromatin modifications and in sperm from the 5'-UTR of full-length LINE-1 elements, but are depleted from lamin-associated heterochromatin. Analysis of microDNAs from a set of human cancer cell lines revealed lineage-specific patterns of microDNA origins. A survey of microDNAs from chicken cells defective in various DNA repair proteins reveals that homologous recombination and non-homologous end joining repair pathways are not required for microDNA production. Deletion of the MSH3 DNA mismatch repair protein results in a significant decrease in microDNA abundance, specifically from non-CpG genomic regions. Thus, microDNAs arise as part of normal cellular physiology—either from DNA breaks associated with RNA metabolism or from replication slippage followed by mismatch repair.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
authors.forename: Laura W
authors.initials: LW
authors.lastname: Dillon
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Shibata
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
authors.forename: Yuh-Hwa
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Lineberger Comprehensive Cancer Center, Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27514, USA.
authors.forename: Smaranda
authors.initials: S
authors.lastname: Willcox
authors.affiliation: Lineberger Comprehensive Cancer Center, Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27514, USA.
authors.forename: Jack D
authors.initials: JD
authors.lastname: Griffith
authors.affiliation: Laboratory of Molecular Pharmacology and Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
authors.forename: Yves
authors.initials: Y
authors.lastname: Pommier
authors.affiliation: CREST Research Project, Japan Science and Technology Corporation, Radiation Genetics, Faculty of Medicine, Kyoto University, Konoe Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Takeda
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA. Electronic address: ad8q@virginia.edu.
authors.forename: Anindya
authors.initials: A
authors.lastname: Dutta
mesh_terms: D000818:Animals; D001482:Base Composition; D045744:Cell Line, Tumor; D002645:Chickens; D002843:Chromatin / Q000235:genetics; D018899:CpG Islands; D053843:DNA Mismatch Repair; D004270:DNA, Circular; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000074081:MutS Homolog 3 Protein; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004270:DNA, Circular; C089762:Msh3 protein, mouse; D000074081:MutS Homolog 3 Protein; D011506:Proteins