title: Effects of dihydroxy gymnemic triacetate (DGT) on expression of apoptosis associated proteins in human prostate cancer cell lines (PC3).
abstract: Prostate cancer is the most common malignancies among men. The present study is aimed at the investigation of dihydroxy gymnemic triacetate (DGT) from Gymnema sylvestre on mitochondrial apoptotic pathway and cell cycle arrest. Treatment of DGT resulted in a dose-dependent inhibition of growth of PC-3 cells. The cell cycle arrest was observed at the G2/M phase and accumulation of apoptotic cells was observed in DGT-treated prostate cancer cell lines. The occurrence of apoptosis in these cells was observed by DNA fragmentation. These events were associated with increased levels of pro-apoptotic proteins Bax, Bad and reduced levels of the antiapoptotic proteins Bcl-2, Bcl-xL and Mcl-1. DGT also induces the activation of caspase-9 and caspase-3. The above results, clearly, suggest that DGT induces apoptosis by the intrinsic pathways which could be very useful for the treatment of prostate cancer.
authors.affiliation: a PG & Research Department of Biotechnology & Bioinformatics , Holy Cross College (Autonomous) , Tiruchirapalli , Tamil Nadu , India and.
authors.forename: Rajamanickam
authors.initials: R
authors.lastname: Pon Nivedha
authors.affiliation: a PG & Research Department of Biotechnology & Bioinformatics , Holy Cross College (Autonomous) , Tiruchirapalli , Tamil Nadu , India and.
authors.forename: Jayaraman
authors.initials: J
authors.lastname: Selvaraj
authors.affiliation: b Department of Pharmaceutical Chemistry , Ultra College of Pharmacy , Madurai , Tamil Nadu , India.
authors.forename: Keddal Govindaram
authors.initials: KG
authors.lastname: Lalitha
authors.affiliation: a PG & Research Department of Biotechnology & Bioinformatics , Holy Cross College (Autonomous) , Tiruchirapalli , Tamil Nadu , India and.
authors.forename: Manikkam
authors.initials: M
authors.lastname: Rajalakshmi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D051017:Apoptosis Regulatory Proteins; D006576:Heterocyclic Compounds, 4 or More Rings; D051034:Inhibitor of Apoptosis Proteins; C547930:dihydroxy gymnemic triacetate
keywords: Dihydroxy gymnemic triacetate; Gymnema sylvestre; PC-3; intrinsic; prostate cancer