title: Transmissible microbial and metabolomic remodeling by soluble dietary fiber improves metabolic homeostasis.
abstract: Dietary fibers are increasingly appreciated as beneficial nutritional components. However, a requisite role of gut microbiota in fiber function and the overall impact of fibers on metabolomic flux remain unclear. We herein showed enhancing effects of a soluble resistant maltodextrin (RM) on glucose homeostasis in mouse metabolic disease models. Remarkably, fecal microbiota transplantation (FMT) caused pronounced and time-dependent improvement in glucose tolerance in RM recipient mice, indicating a causal relationship between microbial remodeling and metabolic efficacy. Microbial 16S sequencing revealed transmissible taxonomic changes correlated with improved metabolism, notably enrichment of probiotics and reduction of Alistipes and Bacteroides known to associate with high fat/protein diets. Metabolomic profiling further illustrated broad changes, including enrichment of phenylpropionates and decreases in key intermediates of glucose utilization, cholesterol biosynthesis and amino acid fermentation. These studies elucidate beneficial roles of RM-dependent microbial remodeling in metabolic homeostasis, and showcase prevalent health-promoting potentials of dietary fibers.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston, 6431 Fannin St., Houston, TX 77030.
authors.forename: Baokun
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston, 6431 Fannin St., Houston, TX 77030.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Nohara
authors.affiliation: The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Nadim J
authors.initials: NJ
authors.lastname: Ajami
authors.affiliation: Metabolon Inc., 617 Davis Drive, Durham, NC 27713.
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Michalek
authors.affiliation: The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Tian
authors.affiliation: The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Matthew
authors.initials: M
authors.lastname: Wong
authors.affiliation: RhythMed, 14673 W Birch Lane, Wadsworth, IL 60083.
authors.forename: Susan H
authors.initials: SH
authors.lastname: Losee-Olson
authors.affiliation: The Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Petrosino
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston, 6431 Fannin St., Houston, TX 77030.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Yoo
authors.affiliation: Matsutani America Inc., 500 Park Blvd. Suite 1240, Itasca, IL 60143.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shimomura
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston, 6431 Fannin St., Houston, TX 77030.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers; D001786:Blood Glucose; D016000:Cluster Analysis; D004043:Dietary Fiber; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome; D006706:Homeostasis; D008659:Metabolic Diseases / Q000378:metabolism / Q000382:microbiology; D055442:Metabolome; D055432:Metabolomics; D051379:Mice; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D004043:Dietary Fiber; D011134:Polysaccharides; C008315:maltodextrin