title: Cholesterol regulatory effects and antioxidant activities of protein hydrolysates from zebra blenny (Salaria basilisca) in cholesterol-fed rats.
abstract: This study aims to explore the hypocholesterolemic effects and antioxidative activities of zebra blenny protein hydrolysates (ZBPHs) in rats fed with a hypercholesterolemic diet. The rats were fed during eight weeks a standard laboratory diet (normal rats), a high-cholesterol diet (HCD) (1%) or a HCD and orally treated with ZBPHs or undigested zebra blenny proteins (UZBPs) (400 mg per kg per day). Results showed that a hypercholesterolemic diet induced the increase of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C). Treatment with ZBPHs increased the level of high-density lipoprotein cholesterol (HDL-C) and decreased significantly the levels of TC, TG, and LDL-C. In addition, ZBPH treatment showed significant normalization of thiobarbituric acid-reactive substance (TBARS) levels as well as catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities in renal and hepatic tissues. Furthermore, ZBPHs may also exert significant protective effects on liver and kidney functions, evidenced by a marked decrease in the level of serum urea, uric acid, creatinine, alkaline phosphatase (ALP), and alanine aminotransferase (ALAT). Histological studies confirmed that ZBPHs effectively protected the livers and kidneys against hypercholesterolemia-mediated oxidative damage. Therefore, the study strengthens the hypothesis that ZBPHs can be used as novel antioxidants and hypocholesterolemic compounds against hyperlipidemia induced atherosclerosis.
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173-3038, Sfax, Tunisia. naourez.ktari@yahoo.fr.
authors.forename: Naourez
authors.initials: N
authors.lastname: Ktari
authors.forename: Olfa
authors.initials: O
authors.lastname: Belguith-Hadriche
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ben Amara
authors.forename: Aïda
authors.initials: A
authors.lastname: Ben Hadj
authors.forename: Mouna
authors.initials: M
authors.lastname: Turki
authors.forename: Fatma
authors.initials: F
authors.lastname: Makni-Ayedi
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudaouara
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.forename: Ahmed
authors.initials: A
authors.lastname: Boualga
authors.forename: Riadh
authors.initials: R
authors.lastname: Ben Salah
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D029941:Fish Proteins / Q000737:chemistry / Q000378:metabolism*; D005399:Fishes; D006801:Humans; D006937:Hypercholesterolemia / Q000178:diet therapy* / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D029941:Fish Proteins; D011492:Protein Hydrolysates; D014280:Triglycerides; D002374:Catalase; D013482:Superoxide Dismutase