title: Rescue of Brain Function Using Tunneling Nanotubes Between Neural Stem Cells and Brain Microvascular Endothelial Cells.
abstract: Evidence indicates that neural stem cells (NSCs) can ameliorate cerebral ischemia in animal models. In this study, we investigated the mechanism underlying one of the neuroprotective effects of NSCs: tunneling nanotube (TNT) formation. We addressed whether the control of cell-to-cell communication processes between NSCs and brain microvascular endothelial cells (BMECs) and, particularly, the control of TNT formation could influence the rescue function of stem cells. In an attempt to mimic the cellular microenvironment in vitro, a co-culture system consisting of terminally differentiated BMECs from mice in a distressed state and NSCs was constructed. Additionally, engraftment experiments with infarcted mouse brains revealed that control of TNT formation influenced the effects of stem cell transplantation in vivo. In conclusion, our findings provide the first evidence that TNTs exist between NSCs and BMECs and that regulation of TNT formation alters cell function.
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Geriatrics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Xie
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tian
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Desheng
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China. liu.mingyuan@foxmail.com.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China. yangtaiguan@sina.com.|Department of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. yangtaiguan@sina.com.
authors.forename: Yangtai
authors.initials: Y
authors.lastname: Guan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply* / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000166:cytology*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D015739:Nocodazole / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D015739:Nocodazole
keywords: Brain microvascular endothelial cells; MCAO model; Neural stem cells; Tunneling nanotubes