title: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord.
abstract: Adult rat and human spinal cord neural stem/progenitor cells (NSPCs) cultured in growth factor-enriched medium allows for the proliferation of multipotent, self-renewing, and expandable neural stem cells. In serum conditions, these multipotent NSPCs will differentiate, generating neurons, astrocytes, and oligodendrocytes. The harvested tissue is enzymatically dissociated in a papain-EDTA solution and then mechanically dissociated and separated through a discontinuous density gradient to yield a single cell suspension which is plated in neurobasal medium supplemented with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and heparin. Adult rat spinal cord NSPCs are cultured as free-floating neurospheres and adult human spinal cord NSPCs are grown as adherent cultures. Under these conditions, adult spinal cord NSPCs proliferate, express markers of precursor cells, and can be continuously expanded upon passage. These cells can be studied in vitro in response to various stimuli, and exogenous factors may be used to promote lineage restriction to examine neural stem cell differentiation. Multipotent NSPCs or their progeny can also be transplanted into various animal models to assess regenerative repair.
authors.affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Center; amothe@uhnres.utoronto.ca.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mothe
authors.affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Center; Department of Surgery, Division of Neurosurgery, Toronto Western Hospital and University of Toronto.
authors.forename: Charles H
authors.initials: CH
authors.lastname: Tator
mesh_terms: D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D000818:Animals; D001253:Astrocytes / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology; D051381:Rats; D013116:Spinal Cord / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods; D055815:Young Adult