title: Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection.
abstract: A simple screening method for isolation and determination of the total content of some sulfonamides in milk using solid-phase extraction and a color reaction is described. This procedure is based on SPE of sulfonamides on hypercrosslinked polystyrene, elution with acetonitrile and off-line derivatization with p-dimethylaminocinnamaldehyde in acetonitrile followed by spectrophotometric determination. The reaction produces intense violet-red color and can be easily used both for quantitation of sulfonamides using spectrophotometry and for naked-eye semi-quantitative estimation. Maximum absorption of the reaction product was determined at 540 nm. The Lambert-Beer's law was obeyed in the range of 0.07-3.0 μg mL(-1) in eluate, with the squared correlation coefficient (R(2)) of 0.9875-0.9995, and the relative standard deviation (RSD) of 3-4%. The limits of SAs detection using preconcentration were of 0.02-0.03 μg mL(-1). The proposed method can be recommended as a routine screening method for quantitation of sulfonamides in milk.
authors.affiliation: Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia.
authors.forename: Stanislava G
authors.initials: SG
authors.lastname: Dmitrienko
authors.affiliation: Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kochuk
authors.affiliation: Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia.
authors.forename: Veronika V
authors.initials: VV
authors.lastname: Tolmacheva
authors.affiliation: Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia. Electronic address: apyari@mail.ru.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Apyari
authors.affiliation: Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia.
authors.forename: Yury A
authors.initials: YA
authors.lastname: Zolotov
mesh_terms: D000818:Animals; D002417:Cattle; D002934:Cinnamates / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*; D013449:Sulfonamides / Q000009:adverse effects* / Q000032:analysis / Q000737:chemistry*
chemical_list: D002934:Cinnamates; D013449:Sulfonamides; C018523:4-dimethylaminocinnamaldehyde
keywords: Hypercrosslinked polystyrene; Milk; Solid-phase extraction; Spectrophotometry; Sulfonamides; p-Dimethylaminocinnamaldehyde