title: Relative Strengths of Promoters Provided by Common Mobile Genetic Elements Associated with Resistance Gene Expression in Gram-Negative Bacteria.
abstract: Comparison of green fluorescent protein expression from outward-facing promoters (POUT) of ISAba1, ISEcp1, and ISAba125 revealed approximate equivalence in strength, intermediate between PCS (strong) and PCWTGN-10 (weak) class 1 integron promoter variants, >30-fold stronger than POUT of ISCR1, and >5 times stronger than Ptac. Consistent with its usual role, PCWTGN-10 produces more mRNA from a "downstream" gfp gene transcriptionally linked to a "usual" PCWTGN-10-associated gene cassette than does POUT of ISAba1.
authors.affiliation: Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Kamruzzaman
authors.affiliation: Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Patterson
authors.affiliation: Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Shereen
authors.initials: S
authors.lastname: Shoma
authors.affiliation: Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Ginn
authors.affiliation: Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Sally R
authors.initials: SR
authors.lastname: Partridge
authors.affiliation: Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia jonathan.iredell@sydney.edu.au.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Iredell
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D001618:beta-Lactamases