title: Resistive-Pulse Measurements with Nanopipettes: Detection of Vascular Endothelial Growth Factor C (VEGF-C) Using Antibody-Decorated Nanoparticles.
abstract: Quartz nanopipettes have recently been employed for resistive-pulse sensing of Au nanoparticles (AuNP) and nanoparticles with bound antibodies. The analytical signal in such experiments is the change in ionic current caused by the nanoparticle translocation through the pipette orifice. This paper describes resistive-pulse detection of cancer biomarker (Vascular Endothelial Growth Factor-C, VEGF-C) through the use of antibody-modified AuNPs and nanopipettes. The main challenge was to differentiate between AuNPs with attached antibodies for VEGF-C and antigen-conjugated particles. The zeta-potentials of these types of particles are not very different, and, therefore, carefully chosen pipettes with well-characterized geometry were necessary for selective detection of VEGF-C.
authors.affiliation: †Department of Chemistry and Biochemistry, Queens College-CUNY, Flushing, New York 11367, United States.
authors.forename: Huijing
authors.initials: H
authors.lastname: Cai
authors.affiliation: †Department of Chemistry and Biochemistry, Queens College-CUNY, Flushing, New York 11367, United States.
authors.forename: Yixian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: †Department of Chemistry and Biochemistry, Queens College-CUNY, Flushing, New York 11367, United States.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: †Department of Chemistry and Biochemistry, Queens College-CUNY, Flushing, New York 11367, United States.
authors.forename: Michael V
authors.initials: MV
authors.lastname: Mirkin
authors.affiliation: ‡Department of Chemistry, U-60, University of Connecticut, 55 N. Eagleville Rd., Storrs, Connecticut 06269-3060, United States.
authors.forename: Snehasis
authors.initials: S
authors.lastname: Bhakta
authors.affiliation: ‡Department of Chemistry, U-60, University of Connecticut, 55 N. Eagleville Rd., Storrs, Connecticut 06269-3060, United States.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Bishop
authors.affiliation: ‡Department of Chemistry, U-60, University of Connecticut, 55 N. Eagleville Rd., Storrs, Connecticut 06269-3060, United States.
authors.forename: Amit A
authors.initials: AA
authors.lastname: Joshi
authors.affiliation: ‡Department of Chemistry, U-60, University of Connecticut, 55 N. Eagleville Rd., Storrs, Connecticut 06269-3060, United States.|§Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut, United States.|∥School of Chemistry, National University of Ireland at Galway, Galway, Ireland.
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D013499:Surface Properties; D042582:Vascular Endothelial Growth Factor C / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D042582:Vascular Endothelial Growth Factor C; D006046:Gold