title: Phospholipid hydroperoxide glutathione peroxidase is involved in the maintenance of male fertility under cryptorchidism in mice.
abstract: Severe oxidative stress by cryptorchidism leads to infertility. To assess the functional significance of phospholipid hydroperoxidase glutathione peroxidase (PHGPx) under cryptorchidism, PHGPx expression was spatiotemporally analyzed in testes and epididymis excised at 1, 4, 7, 14, 21, and 28 days after experimental bilateral cryptorchidism in adult mice. In testes, while apoptosis-related caspase 3 and Bcl-xL mRNAs were significantly changed after 14 days, 3 beta-hydroxysteroid dehydrogenase mRNA was greatly reduced immediately after cryptorchidism. Under cryptorchidism, PHGPx was significantly decreased in both organs after 21 days, while its mRNA was greatly reduced in testes after 14 days and in epididymis after 4 days. However, PHGPx was upregulated in degenerative spermatids, multinucleated giant cells, and Leydig cells in testes and desquamous spermatids in epididymis until 21 days, but was weakly detected in the spermatids at 28 days. These findings suggest that PHGPx is necessary for maintenance of male fertility under cryptorchidism in testes.
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Ki Youn
authors.initials: KY
authors.lastname: Jung
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Yon
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: A Young
authors.initials: AY
authors.lastname: Jung
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Jong Geol
authors.initials: JG
authors.lastname: Lee
authors.affiliation: Asan Institute for Life Sciences, Asan Medical Center and University of Ulsan, Seoul 138-736, Republic of Korea.
authors.forename: In-Jeoung
authors.initials: IJ
authors.lastname: Baek
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Beom Jun
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Young Won
authors.initials: YW
authors.lastname: Yun
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea. Electronic address: synam@cbu.ac.kr.
authors.forename: Sang-Yoon
authors.initials: SY
authors.lastname: Nam
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000818:Animals; D053148:Caspase 3 / Q000235:genetics; D003456:Cryptorchidism / Q000378:metabolism* / Q000473:pathology; D004822:Epididymis / Q000378:metabolism* / Q000473:pathology; D005298:Fertility / Q000502:physiology*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008297:Male; D008813:Mice, Inbred ICR; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D051020:bcl-X Protein / Q000235:genetics; D000094765:Glutathione Peroxidase GPX1
chemical_list: C494812:Bcl2l1 protein, mouse; D012333:RNA, Messenger; D051020:bcl-X Protein; D015096:3-Hydroxysteroid Dehydrogenases; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D053148:Caspase 3; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse
keywords: Antioxidant enzyme; Apoptosis; Cryptorchidism; Fertility; Oxidative stress; PHGPx