title: Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17.
abstract: A putative gene for a transcriptional regulator (ophR) was detected near each copy of the duplicated phthalate-degrading operon of Rhodococcus sp. DK17. Sequence analysis and molecular modeling indicate that OphR belongs to the IclR family of transcriptional regulators and possesses the N-terminal DNA-binding and C-terminal effector-binding domains. DNA-binding assays demonstrate that OphR regulates the phthalate operon by binding to the ophA1-ophR intergenic region.
authors.affiliation: Department of Systems Biology, Yonsei University, Seoul, 120-749 Korea ; Department of Systems Biotechnology, Chung-Ang University, Anseong, 456-756 Korea.
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Choi
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Daegu, 702-701 Korea.
authors.forename: Beom Sik
authors.initials: BS
authors.lastname: Kang
authors.affiliation: Korea Basic Science Institute, Seoul Branch, Seoul, 136-701 Korea.
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Nam
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Anseong, 456-756 Korea.
authors.forename: Woo Jun
authors.initials: WJ
authors.lastname: Sul
authors.affiliation: Department of Systems Biology, Yonsei University, Seoul, 120-749 Korea.
authors.forename: Eungbin
authors.initials: E
authors.lastname: Kim
keywords: Phthalate; Regulator; Rhodococcus sp. strain DK17; Transcription initiation