title: Human plasma protein adsorption onto alumina nanoparticles relevant to orthopedic wear.
abstract: OBJECTIVE
  Wear of ceramic orthopedic devices generates nanoparticles in vivo that may present a different biological character from the monolithic ceramic from which they are formed. The current work investigated protein adsorption from human plasma on alumina nanoparticles and monolithic samples representative of both wear particles and the ceramic components as implanted.
  
  
  METHODS
  A physicochemical characterization of the particles and their dispersion state was carried out, and the protein adsorption profiles were analyzed using 1D SDS-PAGE and mass spectrometry.
  
  
  RESULTS
  Significant differences in protein-binding profiles were identified where the nanoparticles selectively bound known transporter proteins rather than the more highly abundant serum proteins that were observed on the monoliths.
  
  
  CONCLUSIONS
  Proteins associated with opsonization of particles were seen to be present in the protein corona of the nanoparticles, which raises questions regarding the role of wear particles in periprosthetic tissue inflammation and aseptic loosening.
authors.affiliation: UCD School of Mechanical and Materials Engineering, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: John P
authors.initials: JP
authors.lastname: Gibbons
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin - Ireland.|Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Marco P
authors.initials: MP
authors.lastname: Monopoli
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin - Ireland.|Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Martin
authors.initials: M
authors.lastname: Lundqvist
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Iker
authors.initials: I
authors.lastname: Montes-Burgos
authors.affiliation: UCD School of Mechanical and Materials Engineering, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Eilis E
authors.initials: EE
authors.lastname: McGrath
authors.affiliation: Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Iseult
authors.initials: I
authors.lastname: Lynch
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
authors.affiliation: UCD School of Mechanical and Materials Engineering, University College Dublin, Belfield, Dublin - Ireland.
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Stanton
mesh_terms: D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D007595:Joint Prosthesis; D053758:Nanoparticles / Q000737:chemistry*; D000066970:Protein Corona / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D000066970:Protein Corona; D000537:Aluminum Oxide