title: Proteomics dedicated to biofilmology: What have we learned from a decade of research?
abstract: Advances in proteomics techniques over the past decade, closely integrated with genomic and physicochemical approach, have played a great role in developing knowledge of the biofilm lifestyle of bacteria. Despite bacterial proteome versatility, many studies have demonstrated the ability of proteomics approaches to elucidating the biofilm phenotype. Though these investigations have been largely used for biofilm studies in the last decades, they represent, however, a very low percentage of proteomics works performed up to now. Such approaches have offered new targets for combating microbial biofilms by providing a comprehensive quantitative and qualitative overview of their protein cell content. Herein, we summarized the state of the art in knowledge about biofilm physiology after one decade of proteomic analysis. In a second part, we highlighted missing research tracks for the next decade, emphasizing the emergence of posttranslational modifications in proteomic studies stemming from recent advances in mass spectrometry-based proteomics.
authors.affiliation: CNRS, UMR 6270, Laboratory "Polymères, Biopolymères, Surfaces", 76820, Mont-Saint-Aignan, France. arbia.khemiri@yahoo.fr.|University of Normandy, UR, Mont-Saint-Aignan, France. arbia.khemiri@yahoo.fr.|PISSARO Proteomic Facility, IRIB, 76820, Mont-Saint-Aignan, France. arbia.khemiri@yahoo.fr.
authors.forename: Arbia
authors.initials: A
authors.lastname: Khemiri
authors.affiliation: CNRS, UMR 6270, Laboratory "Polymères, Biopolymères, Surfaces", 76820, Mont-Saint-Aignan, France.|University of Normandy, UR, Mont-Saint-Aignan, France.|PISSARO Proteomic Facility, IRIB, 76820, Mont-Saint-Aignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: CNRS, UMR 6270, Laboratory "Polymères, Biopolymères, Surfaces", 76820, Mont-Saint-Aignan, France.|University of Normandy, UR, Mont-Saint-Aignan, France.|PISSARO Proteomic Facility, IRIB, 76820, Mont-Saint-Aignan, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D005786:Gene Expression Regulation; D006801:Humans; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D020543:Proteome
keywords: Biofilm; Posttranslational modifications; Proteomics; Signaling; Subcellular proteomics