title: Non-contact intracellular binding of chloroplasts in vivo.
abstract: Non-contact intracellular binding and controllable manipulation of chloroplasts in vivo was demonstrated using an optical fiber probe. Launching a 980-nm laser beam into a fiber, which was placed about 3 μm above the surface of a living plant (Hydrilla verticillata) leaf, enabled stable binding of different numbers of chloroplasts, as well as their arrangement into one-dimensional chains and two-dimensional arrays inside the leaf without damaging the chloroplasts. Additionally, the formed chloroplast chains were controllably transported inside the living cells. The optical force exerted on the chloroplasts was calculated to explain the experimental results. This method provides a flexible method for studying intracellular organelle interaction with highly organized organelle-organelle contact in vivo in a non-contact manner.
authors.affiliation: State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Hongbao
authors.initials: H
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Xiaoshuai
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Li
mesh_terms: D001692:Biological Transport; D002736:Chloroplasts / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018521:Plant Physiological Phenomena