title: miR-30e* is an independent subtype-specific prognostic marker in breast cancer.
abstract: BACKGROUND
  Breast cancer clinical outcome is affected by tumor molecular features, and the identification of subtype-specific prognostic biomarkers is relevant for breast cancer translational research. Gene expression signatures proved to be able to complement prognostic information provided by classical clinico-pathological features. Recently, microRNAs (miRNAs) have been causally linked to tumorigenesis and cancer progression and have been associated with patient outcome, also in breast cancer.
  
  
  METHODS
  MicroRNAs associated with the development of distant metastasis were identified in a cohort of 92 ESR1+/ERBB2- lymph node-negative breast cancers from patients not receiving adjuvant treatment. Results were confirmed and further investigated in a total of 1246 miRNA and gene expression profiles of the Molecular Taxonomy of Breast Cancer International Consortium data set. Moderated t-test, univariable and multivariable Cox regression models were used for statistical analyses.
  
  
  RESULTS
  miR-30e* was identified as independent protective prognostic factor in lymph node-negative untreated patients with ESR1+/ERBB2- tumours and retained a significant association with a good prognosis in treated patients with the same tumor subtype as well as in the ERBB2+ subtype, but not in ESR1-/ERBB2- tumours.
  
  
  CONCLUSIONS
  We highlighted a relevant and subtype-specific role in breast cancer for miR-30e* and demonstrated that adding miRNA markers to gene signatures and clinico-pathological features can help for a better prognostication.
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: D'Aiuto
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Callari
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Dugo
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Merlino
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Musella
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Miodini
authors.affiliation: Department of Pathology, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Venezian, 1 20133 Milan, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Paolini
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Cappelletti
authors.affiliation: Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Amadeo, 42 20133 Milan, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Daidone
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha / Q000032:analysis; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D011379:Prognosis; D016016:Proportional Hazards Models; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D059467:Transcriptome
chemical_list: D047628:Estrogen Receptor alpha; D035683:MicroRNAs; D018719:Erb-b2 Receptor Tyrosine Kinases; C506487:ESR1 protein, human; C546086:MIRN30b microRNA, human; C508053:ERBB2 protein, human