title: Antibacterial Nanofibrous Mats Composed of Eudragit for pH-Dependent Dissolution.
abstract: A pH-responsive nanofibrous mesh was prepared for the controlled release of antibiotics in response to pH changes. Eudragit EPO (EPO) and Eudragit L100 (L100) were injected through inner and outer needle and simultaneously electrospun through coaxial nozzles composed of inner and outer needles. Various amounts of EPO and L100 were coejected with tetracycline through the needle and simultaneously electrospun to the fibrous meshes. The mass erosion rates of the meshes at pH 6.0 gradually decreased as the amounts of EPO increased, whereas those at pH 2.0 showed negligible differences; these differences were confirmed by scanning electron microscopy and monitoring the dry weight changes. At pH 6.0, the fibrous structures of the meshes rapidly disappeared compared to those under acidic conditions because Eudragit L100 is localized to the shell of the nanofiber during the electrospinning process. Both the pH changes and the blend ratio of the two polymers significantly affected the tetracycline release; tetracycline was rapidly released from the meshes at pH 6.0, whereas the release rates were attenuated at pH 2.0. Tetracycline was released faster from the mesh at higher blend ratios of EPO for both pH values. The electrostatic interaction between EPO and L100 is expected to yield different release profiles of tetracycline. Consequently, higher amounts of encapsulated drugs were released from the mesh at neutral pH and successfully inhibited bacterial growth.
authors.affiliation: Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea.
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Son
authors.affiliation: Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea.
authors.forename: Younghee
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea.
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Kyung Hee University, Seoul, 130-701, Republic of Korea.
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Jeong
authors.affiliation: Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea.|Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Republic of Korea.
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000287:Administration, Topical; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012995:Solubility; D055672:Static Electricity; D013752:Tetracycline / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D057968:Transdermal Patch
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011109:Polymethacrylic Acids; C038300:methylmethacrylate-methacrylic acid copolymer; C518398:Eudragit E PO; D013752:Tetracycline
keywords: antiinfectives; drug delivery systems; nanotechnology; pH; polymeric drug delivery systems