title: Transcervical chorionic villus sampling: a practical guide.
abstract: First trimester screening for fetal aneuploidies has made the implementation of diagnostic techniques essential. Chorionic villus sampling (CVS) is the method of choice for obtaining chorionic villi for molecular and cytogenetic analysis in the first trimester. Two techniques have been developed, a transcervical and a transabdominal. The selection criteria have been based historically on factors, such as placental location, parity, maternal weight and preference of the operator. In our institution, we developed an elevated level of expertise in the field of transcervical approach, resulting in good quality of samples and comparable fetal loss rate to other approaches. Despite three decades of transcervical CVS performance, little consensus in terms of its technique and clinical guidelines exists. Considering the expertise and the volume of procedures performed at our center, we suggest a practical clinical guideline for transcervical CVS.
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Iosifina
authors.initials: I
authors.lastname: Stergiotou
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Borobio
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Bennasar
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Goncé
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Mula
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Nuruddin
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Soler
authors.affiliation: a Prenatal Diagnosis Unit, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic, University of Barcelona , Barcelona , Catalonia , Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Borrell
mesh_terms: D000782:Aneuploidy; D002584:Cervix Uteri / Q000000981:diagnostic imaging; D015193:Chorionic Villi Sampling / Q000009:adverse effects / Q000379:methods*; D005260:Female; D006801:Humans; D056888:Patient Positioning; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D059285:Pregnancy, Twin; D011296:Prenatal Diagnosis; D014463:Ultrasonography; D014599:Uterus / Q000000981:diagnostic imaging
keywords: Aneuploidy; chorionic villus sampling; genetic testing; practical guide; prenatal diagnosis