title: A fusion protein derived from Moraxella catarrhalis and Neisseria meningitidis aimed for immune modulation of human B cells.
abstract: Moraxella IgD-binding protein (MID) is a well characterized trimeric autotransporter that specifically targets the IgD of B cells. We fused the membrane anchor of the meningococcal autotransporter NhhA with the IgD-binding region of MID (aa 962-1200) to create a chimeric protein designated as NID. The aim was to use this specific targeting to provide a better vaccine candidate against meningococci, in particular serogroup B by enhancing the immunogenicity of NhhA. NID was thereafter recombinantly expressed in E. coli. The NID-expressing E. coli bound to peripheral B lymphocytes that resulted in cellular activation. Furthermore, we also successfully expressed NID on outer membrane vesicles, nanoparticles that are commonly used in meningococcal vaccines. This study thus highlights the applicability of the menigococcal-Moraxella fusion protein NID to be used for specific targeting of vaccine components to the IgD B cell receptor.
authors.affiliation: a Clinical Microbiology; Department of Translational Medicine; Lund University ; Malmö , Sweden.
authors.forename: Oindrilla
authors.initials: O
authors.lastname: Mukherjee
authors.forename: Birendra
authors.initials: B
authors.lastname: Singh
authors.forename: Burcu
authors.initials: B
authors.lastname: Bayrak
authors.forename: Ann-Beth
authors.initials: AB
authors.lastname: Jonsson
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.forename: Kristian
authors.initials: K
authors.lastname: Riesbeck
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology* / Q000382:microbiology; D001422:Bacterial Adhesion; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007155:Immunologic Factors / Q000235:genetics / Q000378:metabolism*; D001936:Moraxella catarrhalis / Q000235:genetics / Q000276:immunology*; D009345:Neisseria meningitidis / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D007155:Immunologic Factors; D011993:Recombinant Fusion Proteins
keywords: B cells; MID; Neisseria meningitidis; NhhA; OMV; vaccine