title: SNP in pre-miR-1666 decreases mature miRNA expression and is associated with chicken performance.
abstract: Polymorphisms in miRNA genes could potentially alter various biological processes by influencing the processing and (or) target selection of miRNAs. The rs14120863 (C > G) mutation, which we characterized in a Gushi-Anka F2 resource population, resides in the precursor region of miR-1666. Association analysis with chicken carcass and growth traits showed that the SNP was significantly associated with carcass weight, evisceration weight, breast muscle weight, leg muscle weight, and body weight at 8 weeks of age, as well as some body size indexes including shank girth, chest breadth, breast bone length, and body slanting length, in the Gushi-Anka F2 resource population. Quantitative RT-PCR results showed that miR-1666 expression levels in muscle tissues differed within various genotypes. Experiment in DF1 cells further confirmed that the SNP in miR-1666 could significantly alter mature miRNA production. Subsequently, using dual-luciferase report assay, we verified that miR-1666 could perform its function through targeting of the CBFB gene. In conclusion, the SNP in the precursor of miR-1666 could significantly reduce mature miR-1666 production. It may further affect the function of miR-1666 through the target gene CBFB, hence it is associated with chicken growth traits.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Shan-He
authors.initials: SH
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Shun-Hong
authors.initials: SH
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Sun
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Lyu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Zhuan-Jian
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, P.R. China.
authors.forename: Xiang-Tao
authors.initials: XT
authors.lastname: Kang
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics; D002460:Cell Line; D002645:Chickens / Q000235:genetics*; D050658:Core Binding Factor beta Subunit / Q000235:genetics; D005260:Female; D005838:Genotype; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050658:Core Binding Factor beta Subunit; D035683:MicroRNAs
keywords: CBFB; SNP; biogenesis; biogenèse; chicken; microARN; microRNA; poulet