title: Metadherin is required for the proliferation, migration, and invasion of esophageal squamous cell carcinoma and its meta-analysis.
abstract: Metadherin (MTDH) was found to be highly expressed in various squamous cell carcinomas (SCCs); however, meta-analysis evaluating the association of MTDH in SCC has not been performed. The purpose of this study was to explore the biological functions of MTDH in esophageal squamous cell carcinoma (ESCC) and to meta-analyze the association between MTDH and SCC. Immunohistochemistry was performed to examine MTDH expression using an ESCC tissue array consisting of 86 ESCC and 78 paired normal adjacent tissues (NATs). MTDH was significantly overexpressed in ESCC tissues compared with NATs and was significantly associated with lymph node metastasis, differentiation, and prognosis. Knockdown of MTDH using an MTDH-short hairpin RNA plasmid caused cell cycle arrest at the G0/G1 phase and induced apoptosis of EC9706 cells. Knockdown of MTDH suppressed the proliferation, invasion, and migration of ESCC cells. Furthermore, meta-analysis revealed that overexpression of MTDH was significantly associated with the lymph node metastasis, advanced clinical stage, and T classification of tissues in SCC, suggesting that MTDH might be used as a potential therapeutic target in the lymph node metastasis of ESCC.
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Yang
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Mang
authors.initials: M
authors.lastname: Lu
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dai
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Gao
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Ilyar
authors.initials: I
authors.lastname: Sheyhidin
authors.affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China. Electronic address: luxiaomei88@163.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Lu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D016601:RNA-Binding Proteins; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins