title: Effect of Chromium Supplementation on Element Distribution in a Mouse Model of Polycystic Ovary Syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder and one of the most common causes of anovulatory infertility. In addition, insulin resistance is commonly associated with PCOS and contributed to pathophysiology connected to dietary minerals including chromium (Cr), copper (Cu), iron (Fe), and zinc (Zn). The aims of this study were to explore whether PCOS in mice alters levels of these elements and determine if Cr supplementation resolves changes. Twenty-four female BALB/c mice were divided into three groups of eight mice [normal control (NC), PCOS+placebo milk (PP), and PCOS+Cr-containing milk (PCr)]. Each group received a high-fat diet for 4 weeks. Our results show significantly higher levels of dehydroepiandrosterone (DHEA) (p<0.001), fasting glucose (p<0.05), and fasting insulin (p<0.05) in the PP group compared with both NC and PCr group. However, Cr levels were significantly lower in muscle, bone, and serum in the PP group (p<0.05) compared with NC and PCr groups. In liver, bone, and serum, Fe levels were significantly higher in the PP group compared with the NC group (p<0.05). In addition, we found significant correlations between Cu/Zn ratio and fasting insulin in all mice (r=0.61; p=0.002). Given that significant research shows that Cr supplementation improves fasting glucose, fasting insulin, and metal metabolism disorders for PCOS mice, our data suggest that trace element levels can serve as biomarkers to prescribe therapeutic supplementation to maintain a healthy metabolic balance and treat disease conditions.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, 402, Taiwan.
authors.forename: Tsung-Sheng
authors.initials: TS
authors.lastname: Chen
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, 402, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Chen
authors.affiliation: Department of Research and Development, Maxluck Biotechnology Corporation, Taichung, 402, Taiwan.
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Department of Research and Development, Maxluck Biotechnology Corporation, Taichung, 402, Taiwan.
authors.forename: Chi-Ching
authors.initials: CC
authors.lastname: Sun
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, 402, Taiwan. fcmao@nchu.edu.tw.
authors.forename: Frank Chiahung
authors.initials: FC
authors.lastname: Mao
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D001842:Bone and Bones / Q000378:metabolism; D002857:Chromium / Q000008:administration & dosage* / Q000032:analysis; D003300:Copper / Q000032:analysis; D004032:Diet; D059305:Diet, High-Fat; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007501:Iron / Q000032:analysis; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy*; D015032:Zinc / Q000032:analysis
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D007328:Insulin; D002857:Chromium; D003300:Copper; D007501:Iron; D015032:Zinc
keywords: Chromium; Copper; Insulin resistance; Iron; Polycystic ovary syndrome; Zinc