title: Effects of α,β-unsaturated lactones on larval survival and gut trypsin as well as oviposition response of Aedes aegypti.
abstract: Lactones are organic cyclic esters that have been described as larvicides against Aedes aegypti and as components of oviposition pheromone of Culex quinquefasciatus. This work describes the effect of six α,β-unsaturated lactones (5a-5f) on survival of A. aegypti fourth instar larvae (L4). It is also reported the effects of the lactones on L4 gut trypsin activity and oviposition behavior of A. aegypti females. Five lactones were able to kill L4 being the lactones 5a (LC50 of 39.05 ppm), 5e (LC50 of 36.30 ppm) and 5f (LC50 of 40.46 ppm) the most promising larvicides. Only the lactone 5a inhibited L4 gut trypsin activity, with an IC50 of 115.15 µg/mL. Lactones 5a, 5c, 5d and 5e did not exert deterrent or stimulatory effects on oviposition, whereas lactone 5b exhibited a strong deterrent oviposition activity. In conclusion, this work introduces new α,β-unsaturated lactones as promising alternatives to control A. aegypti dissemination. The larvicidal mechanism of the lactone 5a can involve the disruption of proteolysis at larval gut.
authors.affiliation: Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil.
authors.forename: Maria Ester S B
authors.initials: ME
authors.lastname: Barros
authors.affiliation: Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil.
authors.forename: Juliano C R
authors.initials: JC
authors.lastname: Freitas
authors.affiliation: Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil.
authors.forename: Geanne K N
authors.initials: GK
authors.lastname: Santos
authors.affiliation: Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil.
authors.forename: Rayane Cristine Santos
authors.initials: RC
authors.lastname: da Silva
authors.affiliation: Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Rua Dom Manuel de Medeiros, s/n - Dois Irmãos, Recife, PE 52171-900, Brazil.
authors.forename: Emmanuel V
authors.initials: EV
authors.lastname: Pontual
authors.affiliation: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Av. Professor Moraes Rego, s/n - Cidade Universitária, Recife, PE 50670-420, Brazil.
authors.forename: Patrícia M G
authors.initials: PM
authors.lastname: Paiva
authors.affiliation: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Av. Professor Moraes Rego, s/n - Cidade Universitária, Recife, PE 50670-420, Brazil.
authors.forename: Thiago H
authors.initials: TH
authors.lastname: Napoleão
authors.affiliation: Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil. Electronic address: dmafn@ufpe.br.
authors.forename: Daniela M A F
authors.initials: DM
authors.lastname: Navarro
authors.affiliation: Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil.
authors.forename: Paulo H
authors.initials: PH
authors.lastname: Menezes
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D005260:Female; D020128:Inhibitory Concentration 50; D007303:Insect Vectors / Q000187:drug effects* / Q000502:physiology; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D010058:Oviposition / Q000187:drug effects*; D014357:Trypsin / Q000187:drug effects* / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D007306:Insecticides; D007783:Lactones; D014361:Trypsin Inhibitors; D014357:Trypsin
keywords: Aedes aegypti; Gut trypsin; Lactones; Larvicidal; Oviposition