title: Comparative proteomic analysis of Populus trichocarpa early stem from primary to secondary growth.
abstract: Wood is derived from the secondary growth of tree stems. In this study, we investigated the global changes of protein abundance in Populus early stems using a proteomic approach. Morphological and histochemical analyses revealed three typical stages during Populus early stems, which were the primary growth stage, the transition stage from primary to secondary growth and the secondary growth stage. A total of 231 spots were differentially abundant during various growth stages of Populus early stems. During Populus early stem lignifications, 87 differential spots continuously increased, while 49 spots continuously decreased. These two categories encompass 58.9% of all differential spots, which suggests significant molecular changes from primary to secondary growth. Among 231 spots, 165 unique proteins were identified using LC-ESI-Q-TOF-MS, which were classified into 14 biological function groups. The proteomic characteristics indicated that carbohydrate metabolism, oxido-reduction, protein degradation and secondary cell wall metabolism were the dominantly occurring biochemical processes during Populus early stem development. This study helps in elucidating biochemical processes and identifies potential wood formation-related proteins during tree early stem development. It is a comprehensive proteomic investigation on tree early stem development that, for the first time, reveals the overall molecular networks that occur during Populus early stem lignifications.
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors.forename: Jinwen
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Hai
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Shenquan
authors.initials: S
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. Electronic address: yangchuanpingnefu@yahoo.com.
authors.forename: Chuanping
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, United States.
authors.forename: Jack P
authors.initials: JP
authors.lastname: Wang
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Tree Genetics Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. Electronic address: chengyuxiang@nefu.edu.cn.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D010940:Plant Proteins / Q000096:biosynthesis*; D018547:Plant Stems / Q000378:metabolism*; D032107:Populus / Q000378:metabolism*; D020543:Proteome / Q000096:biosynthesis*; D040901:Proteomics
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Early stem development; Populus trichocarpa; Proteomics; Secondary growth