title: A novel and highly specific phage endolysin cell wall binding domain for detection of Bacillus cereus.
abstract: Rapid, specific and sensitive detection of pathogenic bacteria is crucial for public health and safety. Bacillus cereus is harmful as it causes foodborne illness and a number of systemic and local infections. We report a novel phage endolysin cell wall-binding domain (CBD) for B. cereus and the development of a highly specific and sensitive surface plasmon resonance (SPR)-based B. cereus detection method using the CBD. The newly discovered CBD from endolysin of PBC1, a B. cereus-specific bacteriophage, provides high specificity and binding capacity to B. cereus. By using the CBD-modified SPR chips, B. cereus can be detected at the range of 10(5)-10(8) CFU/ml. More importantly, the detection limit can be improved to 10(2) CFU/ml by using a subtractive inhibition assay based on the pre-incubation of B. cereus and CBDs, removal of CBD-bound B. cereus, and SPR detection of the unbound CBDs. The present study suggests that the small and genetically engineered CBDs can be promising biological probes for B. cereus. We anticipate that the CBD-based SPR-sensing methods will be useful for the sensitive, selective, and rapid detection of B. cereus.
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Minsuk
authors.initials: M
authors.lastname: Kong
authors.forename: Jieun
authors.initials: J
authors.lastname: Sim
authors.forename: Taejoon
authors.initials: T
authors.lastname: Kang
authors.forename: Hoang Hiep
authors.initials: HH
authors.lastname: Nguyen
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Park
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
mesh_terms: D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000302:isolation & purification* / Q000821:virology; D001435:Bacteriophages / Q000201:enzymology*; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods; D002473:Cell Wall / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding
chemical_list: D010450:Endopeptidases; C001143:endolysin