title: Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection.
abstract: Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308ΔwbkA) and evaluated its virulence. The survival of 2308ΔwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308ΔwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308ΔwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|School of life sciences, Shangqiu normal university, Shangqiu 476000, China.
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Gui
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Sun
authors.affiliation: College of Biology, Agriculture and Forestry, Tongren University, Tongren 554300, China.
authors.forename: Jun-bo
authors.initials: JB
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
authors.forename: Wen-zhi
authors.initials: WZ
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|College of Co-Innovation Center for Zoonotic Infectious Diseases in the Western Region, Shihezi University, Shihezi 832000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Biology, Agriculture and Forestry, Tongren University, Tongren 554300, China.|College of Medicine, Shihezi University, Shihezi 832000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|College of Co-Innovation Center for Zoonotic Infectious Diseases in the Western Region, Shihezi University, Shihezi 832000, China.
authors.forename: Chuang-fu
authors.initials: CF
authors.lastname: Chen
mesh_terms: D000818:Animals; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D000067996:RAW 264.7 Cells; D014613:Vaccines, Attenuated / Q000276:immunology; D014774:Virulence
chemical_list: D002004:Brucella Vaccine; D014613:Vaccines, Attenuated
keywords: 2308ΔwbkA; Brucella; vaccine