title: Analysis of 24-h recovery of transfused stored RBCs in recipient mice of distinct genetic backgrounds.
abstract: OBJECTIVE
  Human studies have demonstrated substantial donor-to-donor variation in refrigerated RBC storage with respect to several variables, including 24-h post-transfusion RBC recovery. However, the human studies leading to these observations are mostly performed using autologous transfusions of stored RBCs, thereby avoiding issues of infectious disease transmission and alloimmunization. Accordingly, one cannot distinguish whether variability in 24-h RBC recovery is due to alterations in RBC storage, differences in phagocytic activity of the recipient's reticuloendothelial system or both. Similar to humans, genetically distinct inbred mouse strains have substantial differences in RBC storage biology, including 24-h post-transfusion RBC recovery.
  
  
  METHODS
  In this report, we juxtaposed 24-h recoveries in 15 distinct inbred strains of mice, holding the RBC donor constant to isolate transfusion recipient variation as an independent variable. Strains were chosen for differences in baseline reticulocyte count and haemoglobin, which may correlate to RBC life span and turnover.
  
  
  RESULTS
  Unlike large differences observed in storage of RBCs obtained from different strains of mice, only subtle strain-to-strain differences were observed regarding 24-h post-transfusion RBC recoveries.
  
  
  CONCLUSIONS
  These findings indicate that the murine strains examined are not likely to be useful in sorting out mechanisms of clearance of stored RBCs, and suggest that such mechanisms may be generally conserved in the strains of mice analysed.
authors.affiliation: Bloodworks NW Research Institute, Seattle, WA, USA.
authors.forename: H R
authors.initials: HR
authors.lastname: Waterman
authors.affiliation: Bloodworks NW Research Institute, Seattle, WA, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Kapp
authors.affiliation: Bloodworks NW Research Institute, Seattle, WA, USA.
authors.forename: H L
authors.initials: HL
authors.lastname: Howie
authors.affiliation: Laboratory of Transfusion Biology, Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Hod
authors.affiliation: Laboratory of Transfusion Biology, Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Spitalnik
authors.affiliation: Bloodworks NW Research Institute, Seattle, WA, USA.|Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA, USA.|Division of Hematology, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Zimring
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000009:adverse effects*; D017707:Erythrocyte Transfusion / Q000009:adverse effects*; D000068617:Genetic Background; D006454:Hemoglobins / Q000235:genetics / Q000276:immunology; D051379:Mice
chemical_list: D006454:Hemoglobins
keywords: blood components; red cell components; transfusion medicine