title: Discrimination of teas with different degrees of fermentation by SPME-GC analysis of the characteristic volatile flavour compounds.
abstract: As tea is traded all over the world, it is necessary for both customs officers and business investigators to develop an easy and reliable method to discriminate teas from each other. A total of 56 kinds of various green, Oolong, and black teas were collected from different countries and markets, and their catechin contents and volatile flavour compounds (VFC) were compared by analyses, using HPLC and solid-phase microextraction-gas chromatograph (SPME-GC). It was found that neither total catechin nor individual catechin contents in green and Oolong teas were significantly different among the samples investigated, but the fermentation processes altered the profiles of tea VFC. Because many of the individual VFC did not change in response to the fermentation levels, several VFC in combination might be more reliable than a single compound to identify broader ranges of teas. A total concentration of five VFC, trans-2-hexenal, benzaldehyde, methyl-5-hepten-2-one, methyl salicylate, and indole, was shown to be able to discriminate clearly unfermented and fermented teas, while that of trans-2-hexenal and methyl salicylate together supplied an index to differentiate semi- and fully-fermented teas. In addition, the SPME-GC analysis was also able to distinguish real jasmine teas from fake jasmine teas based on the disappearance of some grassy/green odorants.
authors.affiliation: Tea Research Group, Amore-Pacific Corporation R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
authors.forename: Li-Fei
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Department of Food Science and Biotechnology, Kyung Hee University, Seocheon-Dong, Giheung-Gu, Yongin-Si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Joo-Yeon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Tea Research Group, Amore-Pacific Corporation R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
authors.forename: Jin-Oh
authors.initials: JO
authors.lastname: Chung
authors.affiliation: Tea Research Group, Amore-Pacific Corporation R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
authors.forename: Joo-Hyun
authors.initials: JH
authors.lastname: Baik
authors.affiliation: Tea Research Group, Amore-Pacific Corporation R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
authors.forename: Sung
authors.initials: S
authors.lastname: So
authors.affiliation: Department of Food Science and Biotechnology, Kyung Hee University, Seocheon-Dong, Giheung-Gu, Yongin-Si, Gyeonggi-do 446-701, Republic of Korea. Electronic address: winepark@hotmail.com.
authors.forename: Seung-Kook
authors.initials: SK
authors.lastname: Park
keywords: Catechin contents; Fermentation; HPLC; SPME–GC; Tea discrimination; Volatile flavour compounds (VFC)