title: Osteopontin can decrease the cartilage cellular inflammatory reaction induced by LPS.
abstract: OBJECTIVE
  This study aims to explore the effect of osteopontin on the inflammatory reaction of cartilage cells stimulated by LPS and its possible signal pathway.
  
  
  METHODS
  The inflammatory reaction of cartilage cells was induced by LPS, and then the cells were treated with OPN and PD98059 respectively. The expression of TNF-α, IL-1β and ERK1/2 were detected by ELISA or Western-blotting methods.
  
  
  RESULTS
  Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS with dose dependent, while PD98059 could reverse the inhibition of osteopontin.
  
  
  CONCLUSIONS
  Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS, which may be associated with the ERK1/2 signal pathway.
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou 510220, P. R. China.
authors.forename: Fengsheng
authors.initials: F
authors.lastname: Li
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou 510220, P. R. China.
authors.forename: Dongping
authors.initials: D
authors.lastname: Ye
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou 510220, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Duan
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou 510220, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
keywords: ERK1/2 signal pathway; Osteopontin; inflammatory reaction