title: Variation within three apoptosis associated genes as potential risk factors for Achilles tendinopathy in a British based case-control cohort.
abstract: Achilles tendon pathology (ATP) is a degenerative condition which exhibits excessive tenocyte apoptosis. Tumour necrosis factor receptor 1 (TNFR1), caspase-3 (CASP3) and caspase-8 (CASP8) are important regulators of apoptosis. To date, the effects of variation within the genes for TNFR1 and CASP3 as risk factors for ATP have not been described. There is evidence that two single nucleotide polymorphisms (SNPs) within the CASP8 gene are associated with ATP, but only in populations from the Southern Hemisphere. The primary aim of this study was to determine whether SNPs within the TNFRSF1A and CASP3 genes were associated with ATP in British Caucasians. We additionally sought to determine whether copy number variation (CNV) within the CASP8 gene was associated with ATP. We recruited 262 (131 ATP cases and 131 asymptomatic controls) Caucasian participants for this genetic association study and used quantitative PCR with chi-squared (χ(2)) tests and ANOVA to detect significant associations. For our entire cohort, we found no association between the TNFRSF1A rs4149577 (p=0.561), CASP3 rs1049253 (p=0.643) and CASP8 variants (p=0.219) and ATP. Likewise, when we tested potential interactions between gender, genotype and the risk of ATP, we found no association with the variants investigated. In conclusion, the TNFRSF1A, CASP3 and CASP8 gene variants were not associated with ATP in British Caucasians.
authors.affiliation: The Centre for Physical Activity and Chronic Disease, The Institute of Health and Wellbeing, University of Northampton, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Rickaby
authors.affiliation: The Centre for Physical Activity and Chronic Disease, The Institute of Health and Wellbeing, University of Northampton, UK.
authors.forename: Louis
authors.initials: L
authors.lastname: El Khoury
authors.affiliation: The Centre for Physical Activity and Chronic Disease, The Institute of Health and Wellbeing, University of Northampton, UK.
authors.forename: William J
authors.initials: WJ
authors.lastname: Ribbans
authors.affiliation: The Centre for Physical Activity and Chronic Disease, The Institute of Health and Wellbeing, University of Northampton, UK. Electronic address: stuart.raleigh@northampton.ac.uk.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Raleigh
mesh_terms: D000125:Achilles Tendon / Q000293:injuries* / Q000473:pathology; D000328:Adult; D017209:Apoptosis / Q000235:genetics*; D016022:Case-Control Studies; D053148:Caspase 3 / Q000235:genetics*; D053181:Caspase 8 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics*; D012307:Risk Factors; D052256:Tendinopathy / Q000453:epidemiology / Q000235:genetics*; D006113:United Kingdom / Q000453:epidemiology; D044465:White People / Q000235:genetics / Q000706:statistics & numerical data
chemical_list: D047888:Receptors, Tumor Necrosis Factor, Type I; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8
keywords: Achilles; Apoptosis; Genotype; Tendinopathy