title: Changes in Matrix Metalloproteinases in Diabetes Patients' Tears After Vitrectomy and the Relationship With Corneal Epithelial Disorder.
abstract: OBJECTIVE
  Previous studies indicate involvement of matrix metalloproteinases (MMPs) in the pathogenesis of diabetic keratopathy. To evaluate MMP levels in the tears of patients with diabetes, we investigated changes in MMP levels during perioperative periods and clarify the relationship with corneal epithelial disorders following vitrectomy.
  
  
  METHODS
  Matrix metalloproteinase levels in tears were measured by multiplex bead array in patients with or without diabetes who were scheduled for vitrectomy. Twenty-two patients with diabetes and proliferative diabetic retinopathy, and 20 patients with epiretinal membrane or macular hole (control group), were recruited. Changes in MMP levels during perioperative periods and the relationship with corneal epithelial disorders after vitrectomy were analyzed.
  
  
  RESULTS
  The levels of MMP-2, -9, and -10 at 1 day after surgery in the diabetic group were significantly higher than in the control group. At 1 week after surgery, MMP-10 levels in the diabetic group were significantly higher than in the control group. After vitrectomy, corneal epithelial disorders occurred in six patients in the diabetic group but not in the control group. In the diabetic group, MMP-10 levels in tears of patients with corneal epithelial disorders were significantly higher than those in patients without corneal epithelial disorders.
  
  
  CONCLUSIONS
  The MMP concentration in tears of patients with diabetes was higher than in nondiabetic patients after vitrectomy. High MMP-10 levels were observed in patients with diabetes and corneal epithelial disorders after vitrectomy. Aberrant levels of MMP-10 may cause corneal epithelial disorder after vitrectomy.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Matsumura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomomatsu
authors.forename: Shogo
authors.initials: S
authors.lastname: Arimura
authors.forename: Makoto
authors.initials: M
authors.lastname: Gozawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takihara
authors.forename: Masaru
authors.initials: M
authors.lastname: Inatani
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D003920:Diabetes Mellitus / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000601:surgery; D019773:Epiretinal Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D059035:Perioperative Period; D012167:Retinal Perforations / Q000378:metabolism; D013666:Tears / Q000378:metabolism*; D014821:Vitrectomy / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D020782:Matrix Metalloproteinases