title: Applications of Yeast Surface Display for Protein Engineering.
abstract: The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine.
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Cherf
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Cochran
mesh_terms: D061505:Cell Surface Display Techniques / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods; D055550:Protein Stability; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins