title: One pot synthesis and anti-biofilm potential of copper nanoparticles (CuNPs) against clinical strains of Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa, an opportunistic pathogen frequently associated with nosocomial infections, is emerging as a serious threat due to its resistance to broad spectrum antimicrobials. The biofilm mode of growth confers resistance to antibiotics and novel anti-biofilm agents are urgently needed. Nanoparticle based treatments and therapies have been of recent interest because of their versatile applications. This study investigates the anti-biofilm activity of copper nanoparticles (CuNPs) synthesized by the one pot method against P. aeruginosa. Standard physical techniques including UV-visible and Fourier transform infrared spectroscopy, X-ray diffraction and transmission electron microscopy were used to characterize the synthesized CuNPs. CuNP treatments at 100 ng ml(-1) resulted in a 94, 89 and 92% reduction in biofilm, cell surface hydrophobicity and exopolysaccharides respectively, without bactericidal activity. Evidence of biofilm inhibition was also seen with light and confocal microscope analysis. This study highlights the anti-biofilm potential of CuNPs, which could be utilized as coating agents on surgical devices and medical implants to manage biofilm associated infections.
authors.affiliation: a Division of Microbial Biodiversity and Bioenergy, Department of Microbiology, School of Life Sciences , Bharathidasan University , Tiruchirappalli , India.
authors.forename: Felix
authors.initials: F
authors.lastname: LewisOscar
authors.forename: Davoodbasha
authors.initials: D
authors.lastname: MubarakAli
authors.forename: Chari
authors.initials: C
authors.lastname: Nithya
authors.forename: Rajendran
authors.initials: R
authors.lastname: Priyanka
authors.forename: Venkatraman
authors.initials: V
authors.lastname: Gopinath
authors.forename: Naiyf S
authors.initials: NS
authors.lastname: Alharbi
authors.forename: Nooruddin
authors.initials: N
authors.lastname: Thajuddin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D057219:Biofouling / Q000517:prevention & control; D003300:Copper / Q000737:chemistry*; D024881:Drug Resistance, Bacterial; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D003300:Copper
keywords: Pseudomonas aeruginosa; anti-biofilm; cell surface hydrophobicity; copper nanoparticles; exopolysaccharides; nosocomial infections