title: A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins.
abstract: The design and synthesis of a fluorescent sensor of diphosphate esters along with its application for in-gel detection is described. Dinuclear zinc complex 1 selectively binds diphosphate esters in the presence of various other functional groups, including monophosphate esters. Complex 1 also constitutes a competent stain for visualization of pyrophosphorylated proteins in polyacrylamide gels. This reagent will facilitate the validation and exploration of protein pyrophosphorylation.
authors.affiliation: Department of Chemistry, Princeton University , Washington Road, Princeton, New Jersey 08544, United States.
authors.forename: Florence J
authors.initials: FJ
authors.lastname: Williams
authors.affiliation: Department of Chemistry, Princeton University , Washington Road, Princeton, New Jersey 08544, United States.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Fiedler
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D011756:Diphosphates / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D000180:Acrylic Resins; D011756:Diphosphates; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D010748:Phosphopeptides; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; C016680:polyacrylamide gels