title: A Newly Emerged Swine-Origin Influenza A(H3N2) Variant Dampens Host Antiviral Immunity but Induces Potent Inflammasome Activation.
abstract: We compared the innate immune response to a newly emerged swine-origin influenza A(H3N2) variant containing the M gene from 2009 pandemic influenza A(H1N1), termed "A(H3N2)vpM," to the immune responses to the 2010 swine-origin influenza A(H3N2) variant and seasonal influenza A(H3N2). Our results demonstrated that A(H3N2)vpM-induced myeloid dendritic cells secreted significantly lower levels of type I interferon (IFN) but produced significantly higher levels of proinflammatory cytokines and induced potent inflammasome activation. The reduction in antiviral immunity with increased inflammatory responses upon A(H3N2)vpM infection suggest that these viruses have the potential for increased disease severity in susceptible hosts.
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Weiping
authors.initials: W
authors.lastname: Cao
authors.affiliation: Battelle Memorial Institute.
authors.forename: Margarita
authors.initials: M
authors.lastname: Mishina
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Priya
authors.initials: P
authors.lastname: Ranjan
authors.affiliation: Battelle Memorial Institute.
authors.forename: Juan A
authors.initials: JA
authors.lastname: De La Cruz
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Jin Hyang
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Virology, Surveillance, and Diagnosis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Garten
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Amrita
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Microbiology Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
authors.forename: Adolfo
authors.initials: A
authors.lastname: García-Sastre
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Katz
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Shivaprakash
authors.initials: S
authors.lastname: Gangappa
authors.affiliation: Immunology and Pathogenesis Branch.
authors.forename: Suryaprakash
authors.initials: S
authors.lastname: Sambhara
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology* / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D016207:Cytokines; D058847:Inflammasomes
keywords: A(H3N2)vpM; ROS; inflammasome activation; myeloid dendritic cell; proinflammatory cytokines; type I IFN