title: Increasing incidence of base of tongue cancers from 2000 to 2010 due to HPV: the largest demographic study of 210 Danish patients.
abstract: BACKGROUND
  We assessed the development in the number of new base of tongue squamous-cell carcinoma (BSCC) cases per year in eastern Denmark from 2000 to 2010 and whether HPV may explain any observable increased incidence.
  
  
  METHODS
  We performed HPV DNA PCR and p16 immunohistochemistry analysis for all (n=210) BSCCs registered in the Danish Head and Neck Cancer Group (DAHANCA) and the Danish Pathology Data Bank, and genotyped all HPV-positive specimens with amplicon-based next-generation sequencing.
  
  
  RESULTS
  The overall crude incidence of BSCCs increased significantly (5.4% per year) during the study period. This was explained by a significant increase in the number of HPV-positive BSCCs (8.1% per year), whereas the number of HPV-negative BSCCs did not increase significantly. The overall HPV prevalence was 51%, with HPV16 as the predominant HPV type.
  
  
  CONCLUSIONS
  The increased number of HPV-positive BSCCs may explain the increasing incidence of BSCCs in eastern Denmark, 2000-2010.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Garnaes
authors.affiliation: Department of Pathology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Kiss
authors.affiliation: Department of Pathology, Roskilde Hospital, Roskilde, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Andersen
authors.affiliation: Department of Pathology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: M H
authors.initials: MH
authors.lastname: Therkildsen
authors.affiliation: Department of Pathology, Hvidovre Hospital, Hvidovre, Denmark.
authors.forename: M B
authors.initials: MB
authors.lastname: Franzmann
authors.affiliation: Department of Pathology, Naestved/Slagelse Hospital, Region of Zealand, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Filtenborg-Barnkob
authors.affiliation: Department of Pathology, Herlev Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Hoegdall
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: C B
authors.initials: CB
authors.lastname: Lajer
authors.affiliation: Department of Oncology, Herlev Hospital, University of Copenhagen, Copenhagen, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Andersen
authors.affiliation: Department of Oncology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Specht
authors.affiliation: Center for Genomic Medicine, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Joenson
authors.affiliation: Department of Statistics, Bioinformatics and Registry, Danish Cancer Society Research Center, Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Frederiksen
authors.affiliation: Center for Genomic Medicine, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Friis-Hansen
authors.affiliation: Center for Genomic Medicine, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: F C
authors.initials: FC
authors.lastname: Nielsen
authors.affiliation: Department of Virus, Lifestyle and Genes, Danish Cancer Society Research Center and Department of Gynaecology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: S K
authors.initials: SK
authors.lastname: Kjaer
authors.affiliation: Institute of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, Copenhagen, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Norrild
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: von Buchwald
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D003718:Denmark / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D014062:Tongue Neoplasms / Q000453:epidemiology* / Q000821:virology