title: Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging.
abstract: Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other.
authors.affiliation: Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Republic of Korea.
authors.forename: Chang Su
authors.initials: CS
authors.lastname: Lim
authors.affiliation: KU-KIST Graduate School of Converging Science and Technology, Korea University, 1-Anamdong, Seoul, 136-701, Republic of Korea.
authors.forename: Seung Taek
authors.initials: ST
authors.lastname: Hong
authors.affiliation: KU-KIST Graduate School of Converging Science and Technology, Korea University, 1-Anamdong, Seoul, 136-701, Republic of Korea.
authors.forename: Seong Shick
authors.initials: SS
authors.lastname: Ryu
authors.affiliation: Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Republic of Korea. dongeun@korea.ac.kr.
authors.forename: Dong Eun
authors.initials: DE
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Republic of Korea. chobr@korea.ac.kr.
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006624:Hippocampus; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry / Q000502:physiology*; D008853:Microscopy / Q000379:methods*; D008928:Mitochondria / Q000737:chemistry / Q000502:physiology*; D051381:Rats; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes
keywords: fluorescent probes; imaging agents; lysosomes; mitochondria; two photon