title: Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells.
abstract: Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34+ CD31+ endothelial progenitors that can be purified to >95% CD34+ cells via magnetic activated cell sorting (MACS). These CD34+ progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.|Department of Cell and Molecular Biology, Karolinska Institutet, Sweden.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Lian
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Kaitlin K
authors.initials: KK
authors.lastname: Dunn
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Mengxuan
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Tianxiao
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Tongcheng
authors.initials: T
authors.lastname: Qian
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Vijesh J
authors.initials: VJ
authors.lastname: Bhute
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Scott G
authors.initials: SG
authors.lastname: Canfield
authors.affiliation: Department of Chemical & Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Palecek
mesh_terms: D000418:Albumins / Q000737:chemistry*; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D000418:Albumins; D015703:Antigens, CD; D003470:Culture Media