title: PXO_00987, a putative acetyltransferase, is required for flagellin glycosylation, and regulates flagellar motility, exopolysaccharide production, and biofilm formation in Xanthomonas oryzae pv. oryzae.
abstract: Acetyltransferases catalyze an important process for sugar or protein modification. In the genome of Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice, there are 32 acetyltransferase-encoding genes belonging to different families. In this work, we focused on PXO_00987, which encodes a putative acetyltransferase in the flagellar regulon. We found that mutation of PXO_00987 gene abolished the glycosylation of wild-type flagellin protein of Xoo. In addition, the PXO_00987 mutant showed enhanced swimming motility, and decreased exopolysaccharide production and biofilm formation. Virulence assays demonstrated that the PXO_00987 mutant caused shorter disease length on rice leaves, suggesting that the function of PXO_00987 contributes to the pathogenesis of Xoo.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: ftian@ippcaas.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Tian
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: He
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006031:Glycosylation; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D018085:Regulon; D016415:Sequence Alignment; D014774:Virulence; D014974:Xanthomonas / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D005408:Flagellin; D000123:Acetyltransferases
keywords: Bacterial blight of rice; CoA binding sites; Glycosylation island; Virulence