title: Vitamin D Potentiates the Inhibitory Effect of MicroRNA-130a in Hepatitis C Virus Replication Independent of Type I Interferon Signaling Pathway.
abstract: Calcitriol, the bioactive metabolite of vitamin D, was reported to inhibit HCV production in a synergistic fashion with interferon, a treatment in vitro. Our previous study established that miR-130a inhibits HCV replication by restoring the host innate immune response. We aimed to determine whether there is additive inhibitory effect of calcitriol and miR-130a on HCV replication. Here we showed that calcitriol potentiates the anti-HCV effect of miR-130a in both Con1b replicon and J6/JFH1 culture systems. Intriguingly, this potentiating effect of calcitriol on miR-130a was not through upregulating the expression of cellular miR-130a or through increasing the miR-130a-mediated IFNα/β production. All these findings may contribute to the development of novel anti-HCV therapeutic strategies although the antiviral mechanism needs to be further investigated.
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.
authors.forename: Xiaoqiong
authors.identifier: 0000-0002-3556-6739
authors.initials: X
authors.lastname: Duan
authors.affiliation: Guangzhou No. 8 People's Hospital, Guangzhou, China.
authors.forename: Yujuan
authors.identifier: 0000-0003-0263-1247
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.
authors.forename: Yujia
authors.identifier: 0000-0002-2636-5468
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.
authors.forename: Shan
authors.identifier: 0000-0001-6272-4686
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.
authors.forename: Shilin
authors.identifier: 0000-0001-7065-6150
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China ; Toronto General Research Institute, University of Toronto, Toronto, ON, Canada.
authors.forename: Limin
authors.identifier: 0000-0002-7370-6499
authors.initials: L
authors.lastname: Chen
mesh_terms: D002117:Calcitriol / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D014807:Vitamin D / Q000378:metabolism*
chemical_list: D007370:Interferon Type I; D014807:Vitamin D; D002117:Calcitriol