title: Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia.
abstract: Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Colby.Souders@UMassMed.edu.
authors.forename: Colby A
authors.initials: CA
authors.lastname: Souders
authors.affiliation: Department of Medicine, Division of Nephrology, Lehigh Valley Health Network, University of South Florida Morsani College of Medicine, Allentown, PA 18105, USA. sharon_e.maynard@lvhn.org.
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Maynard
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA. jyan1013@gmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Yang.Wang@UMassMed.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. nboatwpi@gmail.com.
authors.forename: Naomi K
authors.initials: NK
authors.lastname: Boatright
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Jessica.Sedan@UMassMed.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Sedan
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. David.Balyozian@UMassMed.edu.
authors.forename: David
authors.initials: D
authors.lastname: Balyozian
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Peter.Cheslock@UMassMed.edu.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Cheslock
authors.affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Deborah.Molrine@UMassMed.edu.
authors.forename: Deborah C
authors.initials: DC
authors.lastname: Molrine
authors.affiliation: Department of Obstetrics and Gynecology, University of Massachusetts Medical School/ UMass Memorial Health Care, Worcester, MA 01605, USA. TiffanyA.MooreSimas@umassmemorial.org.|Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01655, USA. TiffanyA.MooreSimas@umassmemorial.org.
authors.forename: Tiffany A Moore
authors.initials: TA
authors.lastname: Simas
mesh_terms: D017398:Alternative Splicing / Q000276:immunology; D000653:Amniotic Fluid / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D016466:CHO Cells; D003412:Cricetulus; D005260:Female; D006801:Humans; D051379:Mice; D010865:Pilot Projects; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D020033:Protein Isoforms / Q000097:blood / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood* / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D011257:Pregnancy Proteins; D020033:Protein Isoforms; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: diagnostic; isoforms; monoclonal antibody (mAb); preeclampsia; soluble fms-like tyrosine kinase 1 (sFlt1); splice variants