title: Purification and identification of a novel heteropolysaccharide RBPS2a with anti-complementary activity from defatted rice bran.
abstract: A novel heteropolysaccharide RBPS2a with anti-complementary activity was obtained from defatted rice bran by hot water extraction, ethanol precipitation, and purified by gel chromatography after anion-exchange chromatography. This fraction exhibited more potent anti-complementary activity than other polysaccharide fractions. RBPS2a was eluted as a single symmetrical narrow peak on high-performance gel-permeation chromatography (HPGPC) and the average molecular weight was 90,000Da. We found RBPS2a contained 86.7% polysaccharide and 8.7% protein. The amino acid pattern showed that RBPS2a contained large amount of glutamic acid, arginine, aspartic acid, lysine, and alanine. The molar content of the above five amino acids constituted 59.31% of the total amino acids. Gas chromatography of absolute acid hydrolysate of RBPS2a suggested that it was composed of arabinose, xylose, glucose and galactose with a molar ratio of 4:2:1:4. The Fourier-transform infrared spectra (FT-IR) and (1)H, (13)C NMR spectroscopy analysis revealed that RBPS2a had a backbone consisting of β-(1→3)-linked d-galacopyranosyl residues substituted at O-2 with glycosyl residues composed of α-d-xylose-(1→4)-α-d-arabinose-(1→ and α-d-glucose-(1→4)-α-d-arabinose-(1→ linked residues. Furthermore, some of the fractions extracted and purified from defatted rice bran exhibited strong anti-complementary activity. Among these fractions, the purified polysaccharide RBPS2a had the highest activity.
authors.affiliation: School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Biotechnology, Inner Mongolia Agricultural University, ZhaoWuDa Road 306, Hohhot 010018, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China. Electronic address: zxchen_2008@126.com.
authors.forename: Zhengxing
authors.initials: Z
authors.lastname: Chen
keywords: Anti-complementary activity; Heteropolysaccharide; Rice bran