title: Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells.
abstract: The use of micro- and nanodevices as multifunctional systems for biomedical applications has experienced an exponential growth during the past decades. Although a large number of studies have focused on the design and fabrication of new micro- and nanosystems capable of developing multiple functions, a deeper understanding of their interaction with cells is required. In the present study, we evaluated the effect of different microparticle surfaces on their interaction with normal and tumoral human breast epithelial cell lines. For this, AlexaFluor488 IgG functionalized polystyrene microparticles (3 μm) were coated with Polyethyleneimine (PEI) at two different molecular weights, 25 and 750 kDa. The effect of microparticle surface properties on cytotoxicity, cellular uptake and endocytic pathways were assessed for both normal and tumoral cell lines. Results showed a differential response between the two cell lines regarding uptake efficiency and mechanisms of endocytosis, highlighting the potential role of microparticle surface tunning for specific cell targeting.
authors.affiliation: Unitat de Biologia Cel·lular, Departament de Biologia Cel·lular, Fisiologia i Immunologia. Facultat de Biociències. Universitat Autònoma de Barcelona, 08139 Bellaterra, Spain.
authors.forename: Tania
authors.initials: T
authors.lastname: Patiño
authors.affiliation: Unitat de Biologia Cel·lular, Departament de Biologia Cel·lular, Fisiologia i Immunologia. Facultat de Biociències. Universitat Autònoma de Barcelona, 08139 Bellaterra, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Soriano
authors.affiliation: Unitat de Biologia Cel·lular, Departament de Biologia Cel·lular, Fisiologia i Immunologia. Facultat de Biociències. Universitat Autònoma de Barcelona, 08139 Bellaterra, Spain.
authors.forename: Lleonard
authors.initials: L
authors.lastname: Barrios
authors.affiliation: Unitat de Biologia Cel·lular, Departament de Biologia Cel·lular, Fisiologia i Immunologia. Facultat de Biociències. Universitat Autònoma de Barcelona, 08139 Bellaterra, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Ibáñez
authors.affiliation: Unitat de Biologia Cel·lular, Departament de Biologia Cel·lular, Fisiologia i Immunologia. Facultat de Biociències. Universitat Autònoma de Barcelona, 08139 Bellaterra, Spain.
authors.forename: Carme
authors.initials: C
authors.lastname: Nogués
mesh_terms: D000912:Antibodies, Neoplasm / Q000737:chemistry / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000912:Antibodies, Neoplasm; D020099:Coated Materials, Biocompatible; D007074:Immunoglobulin G; D011094:Polyethyleneimine