title: [IL-23 promotes invasion of esophageal squamous cell carcinoma cells by activating DLL4/Notch1 signaling pathway].
abstract: OBJECTIVE
  To investigate the role of interlukin-23 (IL-23) in the invasion of human esophageal squamous cell carcinoma (ESCC) cells and the related mechanism.
  
  
  METHODS
  IL-23 expression in tumor and adjacent tissues from 10 ESCC patients were detected by immunohistochemistry. Real-time fluorescent PCR was used to examine the expressions of Notch1 and Foxn4 mRNAs in different concentration IL-23-treated TE-1 cells. After Notch pathway was blocked with γ-secretase inhibitor DAPT, expressions of Notch intracellular domain (NICD), Delta-like 4 (DLL4), hairy enhancer of split 1 (Hes1), matrix metalloproteinase 9 (MMP-9) in IL-23-treated TE-1 cells were measured by Western blotting. And the migration of IL-23-treated TE-1 cells was studied by TranswellTM migration assay.
  
  
  RESULTS
  Compared with adjacent tissues, IL-23 was highly expressed in ESCC tissues. IL-23 treatment up-regulated significantly the expressions of NICD, DLL4, Hes1 and MMP-9 in TE-1 cells. The blockade of Notch1 pathway inhibited the expressions induced by IL-23. Migration assay revealed that IL-23 treatment significantly enhanced the migration of TE-1 cells.
  
  
  CONCLUSIONS
  IL-23 could promote migration of human ESCC cells by activating DLL4/Notch1 signaling pathway.
authors.affiliation: Medical College, Jiangsu University, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Medical College, Jiangsu University, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Su
authors.affiliation: Medical College, Jiangsu University, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Medical College, Jiangsu University, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xie
authors.affiliation: Medical College, Jiangsu University, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Nuclear Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Mao
authors.affiliation: Institute of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D004938:Esophageal Neoplasms / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053759:Interleukin-23 / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D051881:Receptor, Notch1 / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: C515500:IL23R protein, human; D053759:Interleukin-23; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051881:Receptor, Notch1; D018123:Receptors, Interleukin; C064045:delta protein