title: miR-181c associates with tumor relapse of high grade osteosarcoma.
abstract: High-grade osteosarcoma (OS) is characterized by low incidence, high aggressiveness and moderate 5-years survival rate after aggressive poly-chemotherapy and surgery. Here we used miRNA profiling as a tool to possibly predict and monitor OS's development and therapeutic outcome. First, we evaluated the altered expression of selected miRNAs from a case of Giant Cell Tumor (GCT) apparently evolved into an OS. We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors. Second, we validated the observed miRNA profile in two independent casuistries of ten GCT (not evolved into malignant tumors) and sixteen OS patients. Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients. Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells. These findings indicate that: i) miRNAs aberrantly modulated in GCT could be predictive of its development into OS and ii) miRNAs expression could be useful to monitor the OS therapeutic outcome.
authors.affiliation: Molecular Chemoprevention Unit, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Mori
authors.affiliation: Translational Oncogenomics, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sacconi
authors.affiliation: Translational Oncogenomics, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Canu
authors.affiliation: Translational Oncogenomics, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Ganci
authors.affiliation: Department of Pathology, Catholic University, Rome, Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Novello
authors.affiliation: UOC Radiology, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Anelli
authors.affiliation: UOC Department of Pathology, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Covello
authors.affiliation: Medical Oncology A, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Virginia
authors.initials: V
authors.lastname: Ferraresi
authors.affiliation: Department of Oncology, McMaster University, Hamilton, ON, Canada.
authors.forename: Paola
authors.initials: P
authors.lastname: Muti
authors.affiliation: UOC Orthopedic Surgery, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Biagini
authors.affiliation: Translational Oncogenomics, Regina Elena National Cancer Institute, Rome, Italy.|Department of Oncology, McMaster University, Hamilton, ON, Canada.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Blandino
authors.affiliation: Molecular Chemoprevention Unit, Regina Elena National Cancer Institute, Rome, Italy.|Department of Oncology, McMaster University, Hamilton, ON, Canada.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Strano
mesh_terms: D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs
keywords: giant cell tumor; miRNA profiling; osteosarcoma; relapse