title: Production of 4-ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: a comparative study.
abstract: A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 μM NAA (α-Naphthaleneacetic acid) and 1 μM KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g(-1)) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora.
authors.forename: Remya
authors.initials: R
authors.lastname: Mohanraj
authors.forename: S
authors.initials: S
authors.lastname: Subha
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D002146:Bony Callus / Q000737:chemistry; D002686:Chitin / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D006801:Humans; D027723:Ipomoea batatas / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D013729:Terpenes; D002686:Chitin; D048271:Chitosan; C001470:4-ipomeanol