title: Sensitive determination of 17β-estradiol in river water using a graphene based electrochemical sensor.
abstract: In this study, a novel material for the electrochemical determination of 17β-estradiol using an electrode based on reduced graphene oxide and a metal complex porphyrin has been applied to environmental monitoring. The electrochemical profile of the proposed electrode was analyzed by differential pulse voltammetry, which showed a shift of the oxidation peak potential of 17β-estradiol to 150mV in a less positive direction compared to the bare reduced graphene oxide electrode. DPV experiments were performed in PBS at pH 7.0 to determine 17β-estradiol without any previous step of extraction, cleanup, or derivatization, in the range of 0.1-1.0μmolL(-1) with a detection limit archived at 5.3nmolL(-1) (1.4μgL(-1)). The proposed sensor was successfully applied in the determination of 17β-estradiol in a river water sample without any purification step and was successfully analyzed under the standard addition method. All the obtained results were in agreement with those from the HPLC procedure.
authors.affiliation: Chemistry Department Federal University of São Carlos, C.P. 676, 13560-970 São Carlos, SP, Brazil.
authors.forename: Fernando C
authors.initials: FC
authors.lastname: Moraes
authors.affiliation: Chemistry Department Federal University of São Carlos, C.P. 676, 13560-970 São Carlos, SP, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Rossi
authors.affiliation: Chemistry Department Federal University of São Carlos, C.P. 676, 13560-970 São Carlos, SP, Brazil.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Donatoni
authors.affiliation: Chemistry Department Federal University of São Carlos, C.P. 676, 13560-970 São Carlos, SP, Brazil.
authors.forename: Kleber T
authors.initials: KT
authors.lastname: de Oliveira
authors.affiliation: Chemistry Department Federal University of São Carlos, C.P. 676, 13560-970 São Carlos, SP, Brazil. Electronic address: ernesto@ufscar.br.
authors.forename: Ernesto C
authors.initials: EC
authors.lastname: Pereira
mesh_terms: D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004958:Estradiol / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D010084:Oxidation-Reduction; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D004958:Estradiol; D006108:Graphite
keywords: 17β-estradiol; Metallic porphyrin; Reduced graphene oxide; River water