title: Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells.
abstract: In spite of the strong expression of Wnt-10b in melanomas, its role in melanoma cells has not been elucidated. In the present study, the biological effects of Wnt-10b on murine B16F10 (B16) melanoma cells were investigated using conditioned medium from Wnt-10b-producing COS cells (Wnt-CM). After 2 days of culture in the presence of Wnt-CM, proliferation of B16 melanoma cells was inhibited, whereas tyrosinase activity was increased. An in vitro wound healing assay demonstrated that migration of melanoma cells to the wound area was inhibited with the addition of Wnt-CM. Furthermore, evaluation of cellular senescence revealed prominent induction of SA-β-gal-positive senescent cells in cultures with Wnt-CM. Finally, the growth of B16 melanoma cell aggregates in collagen 3D-gel cultures was markedly suppressed in the presence of Wnt-CM. These results suggest that Wnt-10b represses tumor cell properties, such as proliferation and migration of B16 melanoma cells, driving them toward a more differentiated state along a melanocyte lineage.
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Misu
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. Electronic address: oujix@naramed-u.ac.jp.
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Kawai
authors.affiliation: Department of Neurosurgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Nishimura
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors.forename: Fukumi
authors.initials: F
authors.lastname: Nakamura-Uchiyama
authors.affiliation: Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. Electronic address: myoshika@naramed-u.ac.jp.
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000502:physiology; D017077:Culture Media, Conditioned; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051153:Wnt Proteins / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse
keywords: Differentiation; Melanoma; Wnt signal; Wnt-10b