title: Role of CD11b+Gr-1+ myeloid cells in AGEs-induced myocardial injury in a mice model of acute myocardial infarction.
abstract: OBJECTIVE
  Polymorph neutrophils are the predominant inflammatory cells and play a crucial role on the pathogenesis of myocardial injury at the early stage of acute myocardial infarction (AMI). However, the precursors and the differentiation of neutrophils are not fully understood. Here we explored the role of CD11b+Gr-1+ myeloid-derived suppressor cells (MDSCs) on myocardial injury in the absence and presence of advanced glycation end-products (AGEs) in a mice model of AMI.
  
  
  RESULTS
  Male C57BL/6J mice were selected. Fluorescent actived cell sortor (FACS) data demonstrated significantly increased CD11b+Gr-1+ MDSCs both in peripheral blood circulation and in the ischemic myocardium at 24 hours post AMI. Quantitative-real-time PCR results also revealed significantly upregulated CD11b and Ly6G mRNA expression in the ischemic myocardium. AGEs treatment further aggravated these changes in AMI mice but not in sham mice. Moreover, AGEs treatment also significantly increased infarction size and enhanced cardiomyocyte apoptosis. The mRNA expression of pro-inflammatory cytokine IL-6 and iNOS2 was also significantly increased in AMI + AGEs group compared to AMI group.
  
  
  CONCLUSIONS
  These data suggest enhanced infiltration of MDSCs by AGEs contributes to aggravated myocardial injury in AMI mice, which might be one of the mechanisms responsible for severer myocardial injury in AMI patients complicating diabetes.
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Tongqing
authors.initials: T
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Zhongda Hospital Affiliated to Southeast 89#, Dingjiaqiao Road, Nanjing 210009, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Cardiology, Zhongda Hospital Affiliated to Southeast 89#, Dingjiaqiao Road, Nanjing 210009, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Cardiology, Zhongda Hospital Affiliated to Southeast 89#, Dingjiaqiao Road, Nanjing 210009, China.
authors.forename: Genshan
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Yachen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.
authors.forename: Chengxing
authors.initials: C
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis; D039481:CD11b Antigen / Q000276:immunology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology* / Q000473:pathology*; D009203:Myocardial Infarction / Q000276:immunology* / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000276:immunology
chemical_list: D039481:CD11b Antigen; D017127:Glycation End Products, Advanced; D011956:Receptors, Cell Surface; C551746:granulocyte receptor 1, mouse
keywords: Acute myocardial infarction (AMI); CD11b+Gr-1+ myeloid cells; advanced glycation end-products (AGEs); inflammation; myeloid-derived suppressor cells (MDSCs); pro-inflammatory cytokines