title: Morphokinetics as a predictor of self-correction to diploidy in tripronucleated intracytoplasmic sperm injection-derived human embryos.
abstract: OBJECTIVE
  To describe, in morphokinetic terms, a tripronucleated embryo (TPN) population according to ploidy and to explore the value of such variables for predicting ploidy.
  
  
  METHODS
  Experimental.
  
  
  METHODS
  In vitro fertilization laboratory.
  
  
  METHODS
  Seventy-nine TPN embryos obtained after intracytoplasmic sperm injection (TPN-ICSI) were cultured in a time-lapse incubator for 6 days.
  
  
  METHODS
  Ploidy determinations were carried out for 35 TPN-ICSI at the cleavage and/or blastocyst stage. Their morphokinetics were then retrospectively compared.
  
  
  METHODS
  Direct (cleavage time from 2- to 8-cell stages) and indirect (cell cycle duration and blastomere synchrony at cleavage) morphokinetic variables; ploidy determination by FISH; in vitro development to the blastocyst stage.
  
  
  RESULTS
  TPN-ICSI cleaved later than bipronucleated control embryos (BPN). Diploid TPN displayed morphokinetic behavior closer to BPN than triploid TPN regarding almost all of the direct and indirect morphokinetic variables measured. Variable t5 was found to be a predictable variable of ploidy in TPN.
  
  
  CONCLUSIONS
  TPN-ICSI are not homogeneous in ploidy, cleavage, or morphokinetic terms. Diploid, but nontriploid, TPN are morphokinetically similar to diploid BPN. The ploidy of TPN can be predicted by variable t5.
authors.affiliation: IVF Laboratory, University Institute IVI Valencia, Valencia, Spain.
authors.forename: Noelia
authors.initials: N
authors.lastname: Grau
authors.affiliation: IVF Laboratory, University Institute IVI Valencia, Valencia, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Escrich
authors.affiliation: IVF Laboratory, University Institute IVI Valencia, Valencia, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Galiana
authors.affiliation: IVF Laboratory, University Institute IVI Valencia, Valencia, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: Meseguer
authors.affiliation: PGD Molecular Cytogenetics Unit, Igenomix, Valencia, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: García-Herrero
authors.affiliation: IVF Laboratory, University Institute IVI Valencia, Valencia, Spain; Department of Pediatrics, Obstetrics and Gynecology, University School of Medicine, Valencia University, Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Remohí
authors.affiliation: IVF Laboratory, University Institute IVI Valencia, Valencia, Spain. Electronic address: mariajose.escriba@ivi.es.
authors.forename: María-José
authors.initials: MJ
authors.lastname: Escribá
mesh_terms: D001755:Blastocyst / Q000473:pathology*; D002453:Cell Cycle; D002467:Cell Nucleus / Q000473:pathology*; D002970:Cleavage Stage, Ovum / Q000473:pathology*; D004171:Diploidy; D046149:Embryo Culture Techniques; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007700:Kinetics; D008297:Male; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D059008:Time-Lapse Imaging
keywords: Tripronucleated; diploidy; human; morphokinetics; time-lapse; triploidy