title: Sphingopyxis fribergensis sp. nov., a soil bacterium with the ability to degrade styrene and phenylacetic acid.
abstract: Strain Kp5.2(T) is an aerobic, Gram-negative soil bacterium that was isolated in Freiberg, Saxony, Germany. The cells were motile and rod-shaped. Optimal growth was observed at 20-30 °C. The fatty acids of strain Kp5.2(T) comprised mainly C18 : 1ω7c and summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH). The major respiratory quinone was Q-10. The major polar lipids of strain Kp5.2(T) were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.7%. Sequencing of the 16S rRNA gene of strain Kp5.2(T) allowed its classification into the family Sphingomonadaceae, and the sequence showed the highest similarity to those of members of the genus Sphingopyxis, with Sphingopyxis italica SC13E-S71(T) (99.15% similarity), Sphingopyxis panaciterrae Gsoil 124(T) (98.96%), Sphingopyxis chilensis S37(T) (98.90%) and Sphingopyxis bauzanensis BZ30(T) (98.51%) as the nearest neighbours. DNA-DNA hybridization and further characterization revealed that strain Kp5.2(T) can be considered to represent a novel species of the genus Sphingopyxis. Hence, the name Sphingopyxis fribergensis sp. nov. is proposed, with the type strain Kp5.2(T) ( = DSM 28731(T) = LMG 28478(T)).
authors.affiliation: Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany.
authors.forename: Michel
authors.initials: M
authors.lastname: Oelschlägel
authors.affiliation: Technologieplattform Genomik, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
authors.affiliation: Technologieplattform Genomik, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.affiliation: Institut für Keramik, Glas- und Baustofftechnik, TU Bergakademie Freiberg, Agricolastr. 17, 09599 Freiberg, Germany.
authors.forename: Gert
authors.initials: G
authors.lastname: Schmidt
authors.affiliation: Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schlömann
authors.affiliation: Interdisciplinary Ecological Center, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Tischler
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005858:Germany; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010648:Phenylacetates / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020058:Styrene / Q000378:metabolism*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010648:Phenylacetates; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D020058:Styrene; C025136:phenylacetic acid; C025204:Ubiquinone Q2