title: Chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin A.
abstract: In this work, a chemiluminescence (CL) immunoassay with targeted inhibition was developed for the determination of toxins in food products. For sample treatment, amine-functionalized magnetic nanoparticles (MNPs) were synthesized to extract target molecules, and horseradish peroxidase (HRP) tagged on an antibody was used as a label for CL reaction. In particular, amine-targeted inhibition using aldehyde, i.e., specifically capping the amine with an alkyl group, was developed for a non-specific extraction platform to lower background and improve signal-to-background ratio. For demonstration, ochratoxin A (OTA) was determined in rice using a lab-built drop-type chemiluminescence (DCL) system with luminol-H2O2 reagent. The obtained limit of detection was 1.39 pg mL(-1), which was about 7.3 times better than that of ELISA. Recovery of the method in the range of 87-99% was observed, which was compared with ELISA.
authors.affiliation: Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Sumin
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do, Republic of Korea. Electronic address: plasma@dankook.ac.kr.
authors.forename: H B
authors.initials: HB
authors.lastname: Lim
mesh_terms: D000586:Amination; D005516:Food Microbiology / Q000379:methods; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D012275:Oryza / Q000382:microbiology*
chemical_list: D058185:Magnetite Nanoparticles; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D008165:Luminol; D006861:Hydrogen Peroxide
keywords: Chemiluminescence; Determination of ochrotoxin A; Immunoassay; Inhibition; Magnetic nanoparticles