title: COX-2 induces lytic reactivation of EBV through PGE2 by modulating the EP receptor signaling pathway.
abstract: Inflammation is one of the predisposing factors known to be associated with Epstein Barr Virus (EBV) mediated tumorigenesis. However it is not well understood whether inflammation in itself plays a role in regulating the life cycle of this infectious agent. COX-2, a key mediator of the inflammatory processes is frequently over-expressed in EBV positive cancer cells. In various tumors, PGE2 is the principle COX-2 regulated downstream product which exerts its effects on cellular processes through the EP1-4 receptors. In this study, we further elucidated how upregulated COX-2 levels can modulate the events in EBV life cycle related to latency-lytic reactivation. Our data suggest a role for upregulated COX-2 on modulation of EBV latency through its downstream effector PGE2. This study demonstrates a role for increased COX-2 levels in modulation of EBV latency. This is important for understanding the pathogenesis of EBV-associated cancers in people with chronic inflammatory conditions.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India.
authors.forename: Jaya
authors.initials: J
authors.lastname: Gandhi
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Gaur
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India.
authors.forename: Lohit
authors.initials: L
authors.lastname: Khera
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India. Electronic address: rkaul@south.du.ac.in.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Kaul
authors.affiliation: Department of Microbiology and Tumour Virology Program, Abramson Cancer Center, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA. Electronic address: erle@upenn.edu.
authors.forename: Erle S
authors.initials: ES
authors.lastname: Robertson
mesh_terms: D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D064368:Healthy Volunteers; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D018078:Receptors, Prostaglandin E / Q000378:metabolism*; D015398:Signal Transduction; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D018078:Receptors, Prostaglandin E; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone
keywords: COX-2; EBV; Inflammation; PGE2; Reactivation