title: Burkholderiales participating in pentachlorophenol biodegradation in iron-reducing paddy soil as identified by stable isotope probing.
abstract: As the most prevalent preservative worldwide for many years, pentachlorophenol (PCP) has attracted much interest in the study of biodegradation in soil and aquatic ecosystems. However, the key microorganisms involved in anaerobic degradation are less well understood. Hence, we used DNA-based stable isotope probing (SIP) to identify the PCP-degrading microorganisms in iron-rich paddy soil under anaerobic conditions. (12)C- and (13)C-labeled PCP were almost completely degraded in 30 days under iron-reducing conditions. The results of terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes showed that 197 and 217 bp (HaeIII digests) restriction fragments (T-RFs) were enriched in heavy DNA fractions of (13)C-labeled samples, and the information from 16S rRNA gene clone libraries suggested that the microorganisms corresponding to these T-RF fragments, which increased in relative abundance during incubation, belonged to the order of Burkholderiales, in which 197 and 217 bp were classified as unclassified Burkholderiales and the genus Achromobacter, respectively. The results of the present study indicated that Burkholderiales-affiliated microorganisms were responsible for PCP degradation in anaerobic paddy soil and shed new light on in situ bioremediation in anaerobic PCP contaminated soil.
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tong
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Fangbai
authors.initials: F
authors.lastname: Li
authors.forename: Manjia
authors.initials: M
authors.lastname: Chen
authors.forename: Yahui
authors.initials: Y
authors.lastname: Lv
mesh_terms: D001673:Biodegradation, Environmental; D042521:Burkholderiaceae / Q000378:metabolism; D002247:Carbon Isotopes; D017753:Ecosystem; D004784:Environmental Monitoring; D010416:Pentachlorophenol / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D012987:Soil; D012989:Soil Pollutants; D010416:Pentachlorophenol