title: C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization.
abstract: Three-dimensional organization of the chromatin has important roles in transcription, replication, DNA repair, and pathologic events such as translocations. There are two fundamental ways to study higher-order chromatin organization: microscopic and molecular approaches. In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or "3C" technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques. We further discuss the different types of data generated by the 3C-based methods and how they can be used to answer distinct biological questions.
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: A Rasim
authors.initials: AR
authors.lastname: Barutcu
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fritz
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Sayyed K
authors.initials: SK
authors.lastname: Zaidi
authors.affiliation: Departments of Orthopedic Surgery and Biochemistry & Molecular Biology, Mayo Clinic, Rochester, Massachusetts.
authors.forename: André J
authors.initials: AJ
authors.lastname: van Wijnen
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Jane B
authors.initials: JB
authors.lastname: Lian
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Janet L
authors.initials: JL
authors.lastname: Stein
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Nickerson
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Anthony N
authors.initials: AN
authors.lastname: Imbalzano
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont.
authors.forename: Gary S
authors.initials: GS
authors.lastname: Stein
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004260:DNA Repair / Q000502:physiology*; D004261:DNA Replication / Q000235:genetics*; D016678:Genome / Q000235:genetics*; D006801:Humans; D008853:Microscopy / Q000379:methods
chemical_list: D002843:Chromatin; D004247:DNA