title: Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients.
abstract: BACKGROUND
  Grass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.
  
  
  OBJECTIVE
  To analyze the IgE sensitization profile of a Brazilian grass pollen allergic population using individual allergen molecules.
  
  
  METHODS
  We analyzed sera from 78 grass pollen allergic patients for the presence of IgE antibodies specific for 103 purified micro-arrayed natural and recombinant allergens by chip technology. IgE-ELISA inhibition experiments with Lolium multiflorum, Phleum pratense extracts and a recombinant fusion protein consisting of Phl p 1, Phl p 2, Phl p 5 and Phl p 6 were performed to investigate cross-reactivities.
  
  
  RESULTS
  Within the Brazilian grass pollen allergic patients, the most frequently recognized allergens were Phl p 1 (95%), Phl p 5 (82%), Phl p 2 (76%) followed by Phl p 4 (64%), Phl p 6 (45%), Phl p 11 (18%) and Phl p 12 (18%). Most patients were sensitized only to grass pollen allergens but not to allergens from other sources. A high degree of IgE cross-reactivity between Phleum pratense, Lolium multiflorum and the recombinant timothy grass fusion protein was found.
  
  
  CONCLUSIONS
  Component-resolved analysis of sera from Brazilian grass pollen allergic patients reveals an IgE recognition profile compatible with a typical Pooideae sensitization. The high degree of cross-reactivity between Phleum pratense and Lolium multiflorum allergens suggests that diagnosis and immunotherapy can be achieved with timothy grass pollen allergens in the studied population.
authors.affiliation: Laboratory of Allergy and Clinical Immunology, Biomedical Science Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil; Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Priscila Ferreira de Sousa
authors.initials: PF
authors.lastname: Moreira
authors.affiliation: Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Gangl
authors.affiliation: Department of Medicine, University of Caxias do Sul, Rio Grande do Sul, Brazil.
authors.forename: Francisco de Assis Machado
authors.initials: Fde A
authors.lastname: Vieira
authors.affiliation: Laboratory of Allergy and Clinical Immunology, Biomedical Science Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Leandro Hideki
authors.initials: LH
authors.lastname: Ynoue
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Birgit
authors.initials: B
authors.lastname: Linhart
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Flicker
authors.affiliation: Allergopharma J. Ganzer KG, Reinbek, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Fiebig
authors.affiliation: Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Ines
authors.initials: I
authors.lastname: Swoboda
authors.affiliation: Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Margarete
authors.initials: M
authors.lastname: Focke-Tejkl
authors.affiliation: Laboratory of Allergy and Clinical Immunology, Biomedical Science Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Ernesto Akio
authors.initials: EA
authors.lastname: Taketomi
authors.affiliation: Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Valenta
authors.affiliation: Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Niederberger
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D001938:Brazil; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology*; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D008129:Lolium / Q000378:metabolism*; D008297:Male; D031784:Phleum / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011058:Pollen / Q000276:immunology*; D040081:Protein Array Analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012882:Skin Tests
chemical_list: D000485:Allergens; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E