title: Characterization of Multidrug Resistant Extended-Spectrum Beta-Lactamase-Producing Escherichia coli among Uropathogens of Pediatrics in North of Iran.
abstract: Escherichia coli remains as one of the most important bacteria causing infections in pediatrics and producing extended-spectrum beta-lactamases (ESBLs) making them resistant to beta-lactam antibiotics. In this study we aimed to genotype ESBL-producing E. coli isolates from pediatric patients for ESBL genes and determine their association with antimicrobial resistance. One hundred of the E. coli isolates were initially considered ESBL producing based on their MIC results. These isolates were then tested by polymerase chain reaction (PCR) for the presence or absence of CTX, TEM, SHV, GES, and VEB beta-lactamase genes. About 30.5% of isolated E. coli was ESBL-producing strain. The TEM gene was the most prevalent (49%) followed by SHV (44%), CTX (28%), VEB (8%), and GES (0%) genes. The ESBL-producing E. coli isolates were susceptible to carbapenems (66%) and amikacin (58%) and showed high resistance to cefixime (99%), colistin (82%), and ciprofloxacin (76%). In conclusion, carbapenems were the most effective antibiotics against ESBl-producing E. coli in urinary tract infection in North of Iran. The most prevalent gene is the TEM-type, but the other resistant genes and their antimicrobial resistance are on the rise.
authors.affiliation: Nosocomial Infection Research Center, Mazandaran University of Medical Sciences, Buali Sina Hospital, Sari, Iran.
authors.forename: Mohammad Sadegh
authors.initials: MS
authors.lastname: Rezai
authors.affiliation: Department of Clinical Pharmacy, Faculty of Pharmacy, Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Salehifar
authors.affiliation: Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Rafiei
authors.affiliation: Department of Pharmacotherapy and Translational Research, University of Florida, Box 100486, Gainesville, FL 32610-0486, USA.
authors.forename: Taimour
authors.identifier: 0000-0001-5040-6365
authors.initials: T
authors.lastname: Langaee
authors.affiliation: Department of Clinical Pharmacy, Faculty of Pharmacy, Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Rafati
authors.affiliation: Fatemeh Zahra Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Kheironesa
authors.initials: K
authors.lastname: Shafahi
authors.affiliation: Department of Pharmacotherapy and Translational Research, University of Florida, Box 100486, Gainesville, FL 32610-0486, USA ; Department of Clinical Pharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Km 18 Khazarabad Road, Khazar Square, Sari, Mazandaran 48471-16548, Iran.
authors.forename: Gohar
authors.identifier: 0000-0001-6258-0395
authors.initials: G
authors.lastname: Eslami
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D015780:Carbapenems / Q000008:administration & dosage; D002648:Child; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000235:genetics / Q000382:microbiology; D005260:Female; D006801:Humans; D007492:Iran; D008297:Male; D014552:Urinary Tract Infections / Q000188:drug therapy* / Q000235:genetics / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D047090:beta-Lactams; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1