title: Micelle Bound Structure and Model Membrane Interaction Studies of the Peptide Hylin a1 from the Arboreal South American Frog Hypsiboas albopunctatus.
abstract: Antimicrobial peptides (AMPs) appear as a promising therapeutic candidate against multiresistant pathogens, because they are able to kill microorganisms and have low toxicity of resistance cells. Hylin a1 (Hy-a1, IFGAILPLALGALKNLIK-NH2) is a peptide extracted from the skin secretion of the frog Hypsiboas albopunctatus, which displays antimicrobial and hemolytic activities. We report here structural studies of Hy-a1 using different techniques such as fluorescence, CD and NMR. Our data showed that Hy-a1 acquires a well defined amphipathic α-helix when interacting with a membrane-like environment. Furthermore, Hy-a1 presented different affinity when compared to membranes of zwitterionic or anionic lipid composition. Finally, we proposed a molecular interaction model of this peptide with micelles.
authors.forename: Eliane S F
authors.initials: ES
authors.lastname: Alves
authors.forename: Edson C
authors.initials: EC
authors.lastname: Junior
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Cilli
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Castro
authors.forename: Wagner
authors.initials: W
authors.lastname: Fontes
authors.forename: Mariana T Q
authors.initials: MT
authors.lastname: de Magalhães
authors.forename: Luciano M
authors.initials: LM
authors.lastname: Lião
authors.affiliation: Instituto de Química, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Caixa Postal 04478, Cep: 70910-000, Asa Norte, Brasília, DF, Brasil. aloliveira@unb.br.
authors.forename: Aline L
authors.initials: AL
authors.lastname: de Oliveira
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D008823:Micelles; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D016062:Porosity; D011898:Ranidae
chemical_list: D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D008567:Membranes, Artificial; D008823:Micelles; C558935:hylin-a1 protein, Hypsiboas albopunctatus