title: Effects of sevoflurane on tight junction protein expression and PKC-α translocation after pulmonary ischemia-reperfusion injury.
abstract: Pulmonary dysfunction caused by ischemia-reperfusion injury is the leading cause of mortality in lung transplantation. We aimed to investigate the effects of sevoflurane pretreatment on lung permeability, tight junction protein occludin and zona occludens 1 (ZO-1) expression, and translocation of protein kinase C (PKC)-α after ischemia-reperfusion. A lung ischemia-reperfusion injury model was established in 96 male Wistar rats following the modified Eppinger method. The rats were divided into four groups with 24 rats in each group: a control (group C), an ischemia-reperfusion group (IR group), a sevoflurane control group (sev-C group), and a sevoflurane ischemia-reperfusion group (sev-IR group). There were three time points in each group: ischemic occlusion for 45 min, reperfusion for 60 min and reperfusion for 120 min; and there were six rats per time point. For the 120-min reperfusion group, six extra rats underwent bronchoalveolar lavage. Mean arterial pressure (MAP) and pulse oxygen saturation (SpO2) were recorded at each time point. The wet/dry weight ratio and lung permeability index (LPI) were measured. Quantitative RT-PCR and Western blot were used to measure pulmonary occludin and ZO-1, and Western blot was used to measure cytosolic and membranous PKC-α in the lung. Lung permeability was significantly increased after ischemia-reperfusion. Sevoflurane pretreatment promoted pulmonary expression of occludin and ZO-1 after reperfusion and inhibited the translocation of PKC-α. In conclusion, sevoflurane pretreatment alleviated lung permeability by upregulating occludin and ZO-1 after ischemia-reperfusion. Sevoflurane pretreatment inhibited the translocation and activation of PKC-α, which also contributed to the lung-protective effect of sevoflurane.
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang City, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chai
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang City, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Long
authors.affiliation: Central Laboratory of Shengjing Hospital, China Medical University, Shenyang City, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang City, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang City, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang City, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang City, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Chen
mesh_terms: D018685:Anesthetics, Inhalation / Q000627:therapeutic use*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008171:Lung Diseases / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008738:Methyl Ethers / Q000627:therapeutic use*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000077149:Sevoflurane; D062826:Zonula Occludens-1 Protein / Q000032:analysis / Q000235:genetics*
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D012333:RNA, Messenger; D062826:Zonula Occludens-1 Protein; D000077149:Sevoflurane; D051571:Protein Kinase C-alpha