title: Nanobodies and recombinant binders in cell biology.
abstract: Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.
authors.affiliation: Department of Biology II, Ludwig Maximilians University Munich and Center for Integrated Protein Science Munich, 82152 Planegg-Martinsried, Germany.
authors.forename: Jonas
authors.initials: J
authors.lastname: Helma
authors.affiliation: Department of Biology, Technical University of Darmstadt, 64287 Darmstadt, Germany.
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Cardoso
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium.
authors.forename: Serge
authors.initials: S
authors.lastname: Muyldermans
authors.affiliation: Department of Biology II, Ludwig Maximilians University Munich and Center for Integrated Protein Science Munich, 82152 Planegg-Martinsried, Germany h.leonhardt@lmu.de.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Leonhardt
mesh_terms: D000818:Animals; D003585:Cell Biology; D005455:Fluorescent Antibody Technique / Q000379:methods; D015870:Gene Expression; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods; D061905:Single-Domain Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D061905:Single-Domain Antibodies