title: Temperature-dependent regulation of gene expression in poly (I:C)-treated Japanese flounder, Paralichthys olivaceus.
abstract: Gene expression profiling of poly (I:C)-treated Japanese flounder, Paralichthys olivaceus, under different temperatures was investigated using microarray analysis. The response was analyzed in spleen tissue at 3 and 24 h post injection (hpi) at 15 °C and 25 °C. A large number of genes in fish treated with poly (I:C) at 25 °C were expressed at 3 hpi, whereas the expression profiles at 24 hpi appeared to be similar to those of the controls. Cluster analysis of the different expression profiles showed three distinct groups of up-regulated genes in fish reared at 15 °C. These were early (3 hpi), early-to-late (3 and 24 hpi), and late (24 hpi) up-regulated genes. These genes included type I IFN-related genes and inflammatory genes. Among the up-regulated genes, most of the type I IFN-related genes played early-to-late- and late-responding genes at 15 °C but early-responding genes at 25 °C. Thus, several up-regulated genes in these groups from the microarray result were further verified by qPCR. These results indicate that the type I IFN gene expressions of P. olivaceus treated with poly (I:C) can be regulated in a temperature-dependent manner.
authors.affiliation: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kittipong
authors.initials: K
authors.lastname: Thanasaksiri
authors.affiliation: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.affiliation: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan. Electronic address: h-kondo@kaiyodai.ac.jp.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005412:Flatfishes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D011070:Poly I-C / Q000008:administration & dosage / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013696:Temperature
chemical_list: D011070:Poly I-C
keywords: Japanese flounder; Microarray; Poly (I:C); Temperature