title: Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice.
abstract: A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank. The premature senescence phenotype appeared at the six-leaf stage and the plant died at the early heading stage. psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content. Furthermore, the expression of senescence-related genes was significantly altered in psd128. The mutant trait was controlled by a single recessive nuclear gene. Using map-based strategy, the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA-type ATPase with 809 amino-acid residuals. A single base substitution at position C2347T in psd128 resulted in a premature stop codon. Functional complementation could rescue the mutant phenotype. In addition, RNA interference resulted in the premature senescence and death phenotype. OsCDC48 was expressed constitutively in the root, stem, leaf and panicle. Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus. OsCDC48 was highly conserved with more than 90% identity in the protein levels among plant species. Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Qi-Na
authors.initials: QN
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.|School of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Song
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Bao-Hua
authors.initials: BH
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D020029:Base Pairing / Q000235:genetics*; D001483:Base Sequence; D016923:Cell Death; D002467:Cell Nucleus / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D002874:Chromosome Mapping; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D058503:Mesophyll Cells / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D010641:Phenotype; D010788:Photosynthesis; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D019655:Quantitative Trait, Heritable; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll
keywords: AAA-ATPase; Oryza sativa; OsCDC48; premature senescence and death