title: Relationship between expression of IGFBP7 and clinicopathological variables in gastric cancer.
abstract: OBJECTIVE
  Insulin-like growth factor binding protein 7 (IGFBP7) is reported to have tumour suppressor function through an IGF-dependent pathway in various malignant tumours. However, the expression of IGFBP7 in adenocarcinoma and its relationship with tumour progression and survival differs among studies. Our aims were to investigate the relationship between the expression of IGFBP7 and clinicopathological variables and outcomes of patients with gastric cancer.
  
  
  METHODS
  Tumour samples were obtained from 219 patients with gastric cancer who underwent gastrectomy. The expression of IGFBP7 protein was examined by immunohistochemical staining. IGFBP7 mRNA levels were analysed using real-time quantitative reverse-transcriptase PCR in 24 of the gastric cancer tumours and in adjacent non-tumour tissues. Correlation of IGFBP7 expression with clinicopathological features was analysed.
  
  
  RESULTS
  The protein expression of IGFBP7 was positively correlated with depth of invasion, lymph node metastasis, distant metastasis or recurrence and pathological stage. High expression of IGFBP7 protein was associated with a significantly worse disease-specific survival (p<0.001) and was an independent prognostic factor in multivariable analysis (HR, 4.8; 95% CI 2.1 to 10.6; p<0.001). The IGFBP7 mRNA level was significantly higher in advanced gastric cancer than in early gastric cancer, in tumours with lymph node metastasis than in tumours without lymph node metastasis, and in tumours with distant metastasis or recurrence than in tumours without distant metastasis or recurrence.
  
  
  CONCLUSIONS
  Overexpression of IGFBP7 was associated with tumour progression and poor survival in gastric cancer. IGFBP7 may play a role in tumour progression in gastric cancer.
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Mikito
authors.initials: M
authors.lastname: Inokuchi
authors.affiliation: Department of Translational Oncology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takagi
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Otsuki
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Fujimori
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Yanaka
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Department of Gastric Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Higuchi
authors.affiliation: Center for Minimally Invasive Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Department of Esophageal Surgery, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kawano
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000556:secondary / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D001706:Biopsy; D016009:Chi-Square Distribution; D005260:Female; D005743:Gastrectomy; D006801:Humans; D007150:Immunohistochemistry; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis* / Q000235:genetics; D053208:Kaplan-Meier Estimate; D016014:Linear Models; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; C102529:insulin-like growth factor binding protein-related protein 1
keywords: GASTRIC CANCER; IGF & IGT BINDING PROTEINS; IMMUNOHISTOCHEMISTRY