title: ACE-I Inhibitory Activity from Phaseolus lunatus and Phaseolus vulgaris Peptide Fractions Obtained by Ultrafiltration.
abstract: The involvement of angiotensin-I-converting enzyme (ACE-I) as one of the mechanisms controlling blood pressure is being studied to find alternative means of control of hypertension on human beings. On the market there are synthetic drugs that can control it, but these can cause undesirable health side effects. In this work was assessed the fractionation by ultrafiltration of the Lima bean (Phaseolus lunatus) and Jamapa bean (Phaseolus vulgaris), protein hydrolysates obtained with Alcalase(®) and Flavourzyme(®) on ACE-I inhibitory activity. Four membranes of different molecular cutoffs (10, 5, 3, and 1 kDa) were used. Fractions that had a higher inhibitory activity in both legumes were denominated as E (<1 kDa) with IC50 of 30.3 and 51.8 μg/mL values for the P. lunatus with Alcalase and Flavourzyme, respectively, and for the Phaseolus vulgaris with Alcalase and Flavourzyme with about 63.8 and 65.8 μg/mL values, respectively. The amino acid composition of these fractions showed residues in essential amino acids, which make a good source of energy and amino acids. On the other hand, the presence of hydrophobic amino acids such as V and P is a determining factor in the ACE-I inhibitor effect. The results suggest the possibility of obtaining and utilizing these peptide fractions in the development and innovation of a functional product that helps with treatment and/or prevention of hypertension.
authors.affiliation: 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México.
authors.forename: David
authors.initials: D
authors.lastname: Betancur-Ancona
authors.affiliation: 2 Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional , México, D.F., México.
authors.forename: Gloria
authors.initials: G
authors.lastname: Dávila-Ortiz
authors.affiliation: 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México.
authors.forename: Luis Antonio
authors.initials: LA
authors.lastname: Chel-Guerrero
authors.affiliation: 2 Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional , México, D.F., México.|3 Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Tuxtepec , Tuxtepec, México.
authors.forename: Juan Gabriel
authors.initials: JG
authors.lastname: Torruco-Uco
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D001794:Blood Pressure / Q000187:drug effects; D006801:Humans; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D020128:Inhibitory Concentration 50; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D027805:Phaseolus / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013045:Species Specificity; D014462:Ultrafiltration
chemical_list: D000596:Amino Acids; D000806:Angiotensin-Converting Enzyme Inhibitors; D010446:Peptide Fragments; D010940:Plant Proteins; D011492:Protein Hydrolysates; D007703:Peptidyl-Dipeptidase A
keywords: ACE-I inhibitors; Phaseolus lunatus; Phaseolus vulgaris; peptide fractions; ultrafiltration