title: TRIM26 functions as a novel tumor suppressor of hepatocellular carcinoma and its downregulation contributes to worse prognosis.
abstract: Hepatocellular carcinoma (HCC) is the one of the most common malignancies worldwide and its prognosis is extremely poor. Tripartite motif (TRIM) proteins play crucial roles in cancer cell biology but the function of tripartite motif 26 (TRIM26) has not been investigated. We demonstrated that low expression level of TRIM26 in tumor samples was significantly correlated with worse prognosis in HCC patients. We also demonstrated its expression level was associated with several clinicopathologic features such as AFP level and T stage of HCC patients. Furthermore, we validated that TRIM26 was significantly downregulated in HCC tissue compared with normal liver tissue. To further clarify the functional role of TRIM26 in HCC, We confirmed that TRIM26 silencing can promote cancer cell proliferation, colony forming, migration and invasion in vitro with HCC cell lines HepG2 and Bel-7402. Then we utilized bioinformatic tool to predict gene influenced by TRIM26, showing TRIM26 could modulate gene sets about cancer cell metabolism. In conclusion, we proved that TRIM26 is a novel tumor suppressor modulating multiple metabolism-related pathways in HCC. To our best knowledge, this is the first study to investigate the function of TRIM26 in cancer biology. Our findings provide useful insight into the mechanism of HCC origin and progression. Moreover, TRIM26 may represent a novel therapeutic target for HCC.
authors.affiliation: Department of General Surgery, The Affiliated Baoan Hospital of Southern Medical University, Shenzhen, Guangdong, 518101, China. Electronic address: wangyichenben@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Central Hospital of Enshi Autonomous of Prefecture, Enshi Clinical College of Wuhan University, Enshi, Hubei, 445000, China. Electronic address: hdu1234@163.com.
authors.forename: Du
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Oncology, Qianjiang Central Hospital, Qianjiang, Hubei, 433100, China. Electronic address: yliang0689@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Urology, The Affiliated Baoan Hospital of Southern Medical University, Shenzhen, Guangdong, 518101, China. Electronic address: tjwb001@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China. Electronic address: brilliant_510@126.com.
authors.forename: Jinfen
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Immunology, School of Basic Medicine, Wuhan University, Wuhan, Hubei, 430071, China. Electronic address: anny9655@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China. Electronic address: 984190619@qq.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Immunology, School of Basic Medicine, Wuhan University, Wuhan, Hubei, 430071, China. Electronic address: 996114664@qq.com.
authors.forename: Weiyang
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China. Electronic address: iamdqs@163.com.
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of General Surgery, The Affiliated Baoan Hospital of Southern Medical University, Shenzhen, Guangdong, 518101, China. Electronic address: 18938027146@126.com.
authors.forename: De
authors.initials: D
authors.lastname: He
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000070584:Tripartite Motif Proteins; C519439:TRIM26 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Hepatocellular carcinoma; Prognosis; TRIM26; Tumor suppressor