title: IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein.
abstract: Interleukin-17A is a newly described proinflammatory cytokine, which plays important roles in autoimmune diseases as well as asthma. In current work, we constructed a recombinant plasmid pMFA42S-Ag85a-IL-17a by inserting fusion gene Ag85a-IL-17a into shuttle vector pMFA42S, which was transformed to Mycobacterium smegmatis by electroporation to obtain recombinant M. smegmatis named rMS-Ag85a-IL-17a. The comparison of growth pattern between M. smegmatis and rMS-Ag85a-IL-17a suggested fusion gene had no significant influence on the growth of strains, and rMS-Ag85a-IL-17a expressed fusion protein Ag85A-IL-17A which had good immunogenicity revealed by Western blot. M. smegmatis and rMS-Ag85a-IL-17a were performed to intranasally immunize mice; then, antibody response in sera was detected by enzyme-linked immunosorbent assay. Our findings demonstrated that rMS-Ag85a-IL-17a could induce specific IL-17A autoantibody in mice, which laid the foundation for further study.
authors.affiliation: Department of Pediatrics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.forename: Liangxia
authors.initials: L
authors.lastname: Wu
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Fan
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
authors.forename: Kang
authors.initials: K
authors.lastname: Wu
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020102:Mycobacterium smegmatis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001323:Autoantibodies; D020381:Interleukin-17; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis