title: Estrogen deprivation does not affect vascular heat shock response in female rats: a comparison with oxidative stress markers.
abstract: Hot flashes, which involve a tiny rise in core temperature, are the most common complaint of peri- and post-menopausal women, being tightly related to decrease in estrogen levels. On the other hand, estradiol (E2) induces the expression of HSP72, a member of the 70 kDa family of heat shock proteins (HSP70), which are cytoprotective, cardioprotective, and heat inducible. Since HSP70 expression is compromised in age-related inflammatory diseases, we argued whether the capacity of triggering a robust heat shock (HS) response would be still present after E2 withdrawal. Hence, we studied the effects of HS treatment (hot tub) in female Wistar rats subjected to bilateral ovariectomy (OVX) after a 7-day washout period. Twelve h after HS, the animals were killed and aortic arches were surgically excised for molecular analyses. The results were compared with oxidative stress markers in the plasma (superoxide dismutase, catalase, and lipoperoxidation) because HSP70 expression is also sensitive to redox regulation. Extracellular (plasma) to intracellular HSP70 ratio, an index of systemic inflammatory status, was also investigated. The results showed that HS response was preserved in OVX animals, as inferred from HSP70 expression (up to 40% rise, p < 0.01) in the aortas, which was accompanied by no further alterations in oxidative stress, hematological parameters, and glycemic control either. This suggests that the lack of estrogen per se could not be solely ascribed as the unique source of low HSP70 expression as observed in long-term post-menopausal individuals. As a consequence, periodic evaluation of HSP70 status (iHSP70 vs. eHSP70) may be of clinical relevance because decreased HS response capacity is at the center of the onset of menopause-related dysfunctions.
authors.affiliation: Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite 500, ICBS, 2° andar, Porto Alegre, RS, 90050-170, Brazil.
authors.forename: Antônio Azambuja
authors.initials: AA
authors.lastname: Miragem
authors.forename: Mirna Stela
authors.initials: MS
authors.lastname: Ludwig
authors.forename: Thiago Gomes
authors.initials: TG
authors.lastname: Heck
authors.forename: Fernanda Giesel
authors.initials: FG
authors.lastname: Baldissera
authors.forename: Analu Bender
authors.initials: AB
authors.lastname: dos Santos
authors.forename: Matias Nunes
authors.initials: MN
authors.lastname: Frizzo
authors.forename: Paulo Ivo
authors.initials: PI
authors.lastname: Homem de Bittencourt
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D010052:Ovariectomy; D018384:Oxidative Stress; D051381:Rats
chemical_list: D015415:Biomarkers; D004967:Estrogens; D018840:HSP70 Heat-Shock Proteins