title: Aptamers Selected to Postoperative Lung Adenocarcinoma Detect Circulating Tumor Cells in Human Blood.
abstract: Circulating tumor cells (CTCs) are rare cells and valuable clinical markers of prognosis of metastasis formation and prediction of patient survival. Most CTC analyses are based on the antibody-based detection of a few epithelial markers; therefore miss an important portion of mesenchymal cancer cells circulating in blood. In this work, we selected and identified DNA aptamers as specific affinity probes that bind to lung adenocarcinoma cells derived from postoperative tissues. The unique feature of our selection strategy is that aptamers are produced for lung cancer cell biomarkers in their native state and conformation without previous knowledge of the biomarkers. The aptamers did not bind to normal lung cells and lymphocytes, and had very low affinity to A549 lung adenocarcinoma culture. We applied these aptamers to detect CTCs, apoptotic bodies, and microemboli in clinical samples of peripheral blood of lung cancer and metastatic lung cancer patients. We identified aptamer-associated protein biomarkers for lung cancer such as vimentin, annexin A2, annexin A5, histone 2B, neutrophil defensin, and clusterin. Tumor-specific aptamers can be produced for individual patients and synthesized many times during anticancer therapy, thereby opening up the possibility of personalized diagnostics.
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Krasnoyarsk Research Center, Siberian Branch Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Galina S
authors.initials: GS
authors.lastname: Zamay
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Krasnoyarsk Research Center, Siberian Branch Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Kolovskaya
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Zamay
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.
authors.forename: Yury E
authors.initials: YE
authors.lastname: Glazyrin
authors.affiliation: Krasnoyarsk Regional Clinical Cancer Center named after A.I. Kryzhanovsky, Krasnoyarsk, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Krat
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Zubkova
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Spivak
authors.affiliation: Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Wehbe
authors.affiliation: Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Ana
authors.initials: A
authors.lastname: Gargaun
authors.affiliation: Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Darija
authors.initials: D
authors.lastname: Muharemagic
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.
authors.forename: Mariia
authors.initials: M
authors.lastname: Komarova
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Grigorieva
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Savchenko
authors.affiliation: Krasnoyarsk Regional Clinical Cancer Center named after A.I. Kryzhanovsky, Krasnoyarsk, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Modestov
authors.affiliation: Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Berezovski
authors.affiliation: Krasnoyarsk State Medical University named after prof V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.|Krasnoyarsk Research Center, Siberian Branch Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Zamay
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D000077192:Adenocarcinoma of Lung; D052157:Aptamers, Nucleotide / Q000737:chemistry; D014408:Biomarkers, Tumor; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D060787:Neoplasm Grading; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D011184:Postoperative Period; D011485:Protein Binding; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D002352:Carrier Proteins