title: Efficient staining of actinomycetoma and eumycetoma grains using henna extract.
abstract: The use of natural, nontoxic, convenient and eco-friendly dyes for histopathological diagnosis avoids some of the synthetic dyes' hazards. I used an aqueous extract of henna at a concentration of 20 g/ml and acidified with acetic acid to stain mycetoma grains. Henna stained mycetoma grains orange-red to brown. The engulfed mycetoma grains within inflammatory cells stained well with henna extract compared to hematoxylin and eosin (H & E) and hexamine silver.
authors.forename: Y M
authors.initials: YM
authors.lastname: Zakout
authors.affiliation: a Department of Histopathology and Cytology , Faculty of Medical Laboratory Sciences, University of Khartoum , Khartoum , Sudan.
authors.forename: S E
authors.initials: SE
authors.lastname: Batran
mesh_terms: D004396:Coloring Agents; D006801:Humans; D008271:Mycetoma / Q000473:pathology*; D009285:Naphthoquinones; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents; D009285:Naphthoquinones; C005090:lawsone
keywords: actinomycetoma; chitin; eosin; eumycetoma; hematoxylin; henna; hexamine silver; lawsone; mycetoma grains