title: Effect of chemical interaction on the bonding strengths of self-etching adhesives to deproteinised dentine.
abstract: OBJECTIVE
  The present study examined (1) the chemical interaction between three self-etching adhesives and sodium hypochlorite (NaOCl)-deproteinised dentine, and (2) the influence of NaOCl treatment on bond strength of self-etching adhesives with/without adhesive functional monomers to dentine.
  
  
  METHODS
  Caries-free dentine disks (control) and those treated with 5.25% NaOCl for 60s were prepared. Xeno V (no functional monomers), G-Bond (containing 4-MET) or S3 Bond (containing 10-MDP) were applied to the NaOCl-treated dentine and either left without further treatment, or rinsed with 100% ethanol or distilled water. Attenuated total reflection (ATR) spectroscopy and field-emission scanning electron microscopy (FE-SEM) were used to evaluate the affinity of functional monomers with deproteinised dentine. Chemical interaction between the functional monomers and deproteinised dentine was evaluated using thin-film X-ray diffraction (TF-XRD). Microtensile bond strength (MTBS) was used to evaluate the mechanical property of the adhesives, either immediately or after thermo-cycling (5-55°C) for 10,000 cycles.
  
  
  RESULTS
  According to the ATR and FE-SEM results, G-Bond and S3 Bond showed stronger affinity to deproteinised dentine than Xeno V even after rinsing with water. TF-XRD showed that chemical interaction between S3 Bond and deproteinised dentine occurred by formation of 10-MDP-Ca salt. Both deproteinisation and thermo-cycling adversely affected the MTBS of Xeno V (P<0.05) but deproteinisation had no significant influence on S3 Bond.
  
  
  CONCLUSIONS
  When bonding to NaOCl-treated dentine, self-etch adhesives containing functional monomers (10-MDP) can maintain immediate and aged bond strengths after 10,000 thermal cycles.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhou
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Yake
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: HongYe
authors.initials: H
authors.lastname: Yang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Endodontics, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA.
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: huangcui@whu.edu.cn.
authors.forename: Cui
authors.initials: C
authors.lastname: Huang
mesh_terms: D001840:Dental Bonding / Q000379:methods*; D003738:Dental Cements / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D003765:Models, Dental; D012973:Sodium Hypochlorite / Q000378:metabolism*; D013057:Spectrum Analysis; D013718:Tensile Strength
chemical_list: D003738:Dental Cements; D017220:Dentin-Bonding Agents; D012973:Sodium Hypochlorite
keywords: Adhesive; Functional monomer; Microtensile bond strengths; Sodium hypochlorite, Self-etching