title: Discrimination of healthy and osteoarthritic articular cartilages by Fourier transform infrared imaging and partial least squares-discriminant analysis.
abstract: Fourier transform infrared imaging (FTIRI) combined with chemometrics algorithm has strong potential to obtain complex chemical information from biology tissues. FTIRI and partial least squares-discriminant analysis (PLS-DA) were used to differentiate healthy and osteoarthritic (OA) cartilages for the first time. A PLS model was built on the calibration matrix of spectra that was randomly selected from the FTIRI spectral datasets of healthy and lesioned cartilage. Leave-one-out cross-validation was performed in the PLS model, and the fitting coefficient between actual and predicted categorical values of the calibration matrix reached 0.95. In the calibration and prediction matrices, the successful identifying percentages of healthy and lesioned cartilage spectra were 100% and 90.24%, respectively. These results demonstrated that FTIRI combined with PLS-DA could provide a promising approach for the categorical identification of healthy and OA cartilage specimens.
authors.affiliation: Nanjing University of Aeronautics and Astronautics, Department of Biomedical Engineering, Nanjing, 210016 Jiangsu, China.
authors.forename: Xue-Xi
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: Nanjing University of Aeronautics and Astronautics, Department of Biomedical Engineering, Nanjing, 210016 Jiangsu, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Yin
authors.affiliation: Nanjing University of Aeronautics and Astronautics, Department of Biomedical Engineering, Nanjing, 210016 Jiangsu, China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Mao
authors.affiliation: Oakland University, Department of Physics and Center for Biomedical Research, Rochester, Michigan 48309, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D003952:Diagnostic Imaging / Q000379:methods*; D016002:Discriminant Analysis; D004285:Dogs; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D016018:Least-Squares Analysis; D010003:Osteoarthritis / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*