title: Effect of tocopherol and acetylsalicylic acid on the biochemical indices of blood in dioxin-exposed rats.
abstract: New sources of dioxins and increased dioxin concentrations in the environment, coupled with their increased bioavailability along the food chain and accumulation in adipose tissues, contribute to various adverse long-term biological effects. The purpose of the study was to determine whether tocopherol protects the CNS by decreasing the pro-inflammatory influence of free radicals generated by TCDD; whether acetylsalicylic acid inhibits the production of inflammatory mediators; and whether the combined administration of tocopherol and acetylsalicylic acid to TCDD-exposed rats has a potential CNS-protective effect. The study included 117 rats divided into 8 groups: 75 female and 12 male Buffalo rats aged 8-10 weeks, weighing 140-160 g; as well as 30 female rats aged 6 weeks and weighing 120 g, which were the offspring of females from each study group. In the experiment, the following substances were used: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), dosed at 5 μg/kg BW and 12.5 μg/kg BW, diluted in a 1% DMSO solution at the concentration of 1 μg/ml; α-tocopherol acetate, dosed at 30 mg/kg BW, in 0.2 ml of oil solution; and acetylsalicylic acid, 50mg/kg BW, suspended in 0.5 ml of starch solution, administered orally using a feeding tube. Pleurisy was induced by an injection of 0.15 ml of 1% carrageenin solution. The use of tocopherol reduces the adverse effects of the inflammatory reaction induced by TCDD. Administering tocopherol improves protein metabolism by reducing protein catabolism, and raises γ-globulin fraction levels. Combined acetylsalicylic acid and tocopherol suppress catabolic processes accompanying inflammation.
authors.affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland. Electronic address: joanna.rosinczuk@umed.wroc.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rosińczuk
authors.affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland. Electronic address: ireneusz.calkosinski@umed.wroc.pl.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Całkosiński
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001769:Blood; D005260:Female; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D051381:Rats; D024505:Tocopherols / Q000494:pharmacology*
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D024505:Tocopherols; D001241:Aspirin
keywords: Ascorbic acid; Biochemical parameters; Dioxin; Inflammatory reaction; Tocopherol