title: Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters.
abstract: We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200 aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancing the sensitivity, throughput, and accuracy of the analysis. Thus, aL-ALBiC broadens the opportunities for single-molecule analysis of various membrane transporters and can be used in pharmaceutical applications such as drug screening.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Soga
authors.affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] PRESTO, Japan Science and Technology, Bunkyo-ku, Tokyo 113-8656, Japan [3] CREST, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Rikiya
authors.initials: R
authors.lastname: Watanabe
authors.affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] CREST, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Noji
mesh_terms: D001692:Biological Transport; D008051:Lipid Bilayers / Q000737:chemistry*; D008956:Models, Chemical
chemical_list: D008051:Lipid Bilayers