title: Research on the change of chemical composition in productive process of Re Du Ning injection by HPLC/Q-TOF MS.
abstract: A high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) was developed for the analysis of chemical composition change in the production process of Re Du Ning injection, a Chinese medicine preparation with a combination of Lonicera japonica Thunb., Gardenia jasminoides Ellis and Artemisia annua L. A total of 90 compounds from raw materials-intermediates-Re Du Ning injection were detected; among them, 55 compounds were identified or tentatively characterized, and the characteristic ions of different types of compounds were described. Based on these studies, the different types of compounds in the various process routes were analyzed. A total of 28 compounds, including seven iridoid glycosides and six monoterpenes from G. jasminoides Ellis, five iridoid glycosides, nine phenolic acids and one unknown compound from L. japonica Thunb., were transferred to Re Du Ning injection, and two unknown compounds were generated in the production process of Re Du Ning injection. The results indicated that the Chinese Medicine Pharmaceutical process control is very important. This method could provide some reference for other Chinese medicine preparations.
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Yan-Jing
authors.initials: YJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Bi
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000032:analysis / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D057889:Iridoid Glycosides; D010636:Phenols; D013729:Terpenes; C000596355:reduning
keywords: HPLC/Q-TOF MS; Re Du Ning injection; chemical composition change; production process; qualitative analysis