title: Vasopressin induces endolymphatic hydrops in mouse inner ear, as evaluated with repeated 9.4 T MRI.
abstract: From histopathological specimens, endolymphatic hydrops has been demonstrated in association with inner ear disorders. Recent studies have observed findings suggestive of hydrops using MRI in humans. Previous studies suggest that vasopressin may play a critical role in endolymph homeostasis and may be involved in the development of Ménière's disease. In this study we evaluate the effect of vasopressin administration in vivo in longitudinal studies using two mouse strains. High resolution MRI at 9.4 T in combination with intraperitoneally delivered Gadolinium contrast, was performed before and after chronic subcutaneous administration of vasopressin via mini-osmotic pumps in the same mouse. A development of endolymphatic hydrops over time could be demonstrated in C57BL6 mice (5 mice, 2 and 4 weeks of administration) as well as in CBA/J mice (4 mice, 2 weeks of administration; 6 mice, 3 and 4 weeks of administration). In most C57BL6 mice hydrops developed first after more than 2 weeks while CBA/J mice had an earlier response. These results may suggest an in vivo model for studying endolymphatic hydrops and corroborates the future use of MRI as a tool in the diagnosis and treatment of inner ear diseases, such as Ménière's disease. MRI may also be developed as a critical tool in evaluating inner ear homeostasis in genetically modified mice, to augment the understanding of human disease.
authors.affiliation: Department of Experimental Medical Sciences, Section for Diabetes, Metabolism and Endocrinology, Lund University, Lund, Sweden. Electronic address: eva.degerman@med.lu.se.
authors.forename: Eva
authors.initials: E
authors.lastname: Degerman
authors.affiliation: Lund University Bioimaging Center, Lund University, Lund, Sweden.
authors.forename: René
authors.initials: R
authors.lastname: In 't Zandt
authors.affiliation: Department of Experimental Medical Sciences, Section for Diabetes, Metabolism and Endocrinology, Lund University, Lund, Sweden.
authors.forename: Ann-Ki
authors.initials: AK
authors.lastname: Pålbrink
authors.affiliation: Department of Otorhinolaryngology, Clinical Sciences, Lund University & Skåne University Hospital, Lund, Sweden.
authors.forename: Måns
authors.initials: M
authors.lastname: Magnusson
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000187:drug effects* / Q000503:physiopathology; D004487:Edema; D018159:Endolymphatic Hydrops / Q000139:chemically induced* / Q000503:physiopathology; D005260:Female; D005682:Gadolinium / Q000737:chemistry; D006706:Homeostasis; D007263:Infusions, Parenteral; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009995:Osmosis; D014667:Vasopressins / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D014667:Vasopressins; D005682:Gadolinium
keywords: Cochlea; Endolymphatic hydrops; Magnetic resonance imaging; Ménière's disease; Vasopressin