title: Structures of Large RNAs and RNA-Protein Complexes: Toward Structure Determination of Riboswitches.
abstract: Riboswitches are widespread and important regulatory elements. They are typically present in the mRNA of the gene under their regulation, where they form complex three-dimensional structures that can bind an effector and regulate either transcription or translation of the mRNA. Structural biology has been essential to our understanding of their ligand recognition and conformational switching mechanisms, but riboswitch determination presents several important complications. Overcoming these challenges requires a synergistic approach using rational design of the constructs and supporting methods to biochemically validate the designs and resulting structures.
authors.affiliation: The Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA.
authors.forename: Jason C
authors.initials: JC
authors.lastname: Grigg
authors.affiliation: The Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA. Electronic address: ailong.ke@cornell.edu.
authors.forename: Ailong
authors.initials: A
authors.lastname: Ke
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D003460:Crystallization; D018360:Crystallography, X-Ray; D005818:Genetic Engineering; D056506:Geobacillus / Q000737:chemistry / Q000235:genetics; D008024:Ligands; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D059370:RNA Folding; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D058928:Riboswitch / Q000235:genetics*; D012436:S-Adenosylmethionine / Q000737:chemistry; D046968:Thermoanaerobacter / Q000737:chemistry / Q000235:genetics
chemical_list: D008024:Ligands; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; D058928:Riboswitch; D012436:S-Adenosylmethionine; D012343:RNA, Transfer
keywords: Ligand binding; Regulation; Sensing; Switch; mRNA