title: Visualization of RNA-Quadruplexes in Live Cells.
abstract: Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.
authors.affiliation: †Institute of Molecular Chemistry, University of Dijon, ICMUB CNRS, UMR6302 Dijon, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Laguerre
authors.affiliation: ‡Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
authors.forename: Kyle
authors.initials: K
authors.lastname: Hukezalie
authors.affiliation: §Dimacell Imaging Ressource Center, UMR PAM, University of Burgundy, Agrosup, 21000 Dijon, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Winckler
authors.affiliation: †Institute of Molecular Chemistry, University of Dijon, ICMUB CNRS, UMR6302 Dijon, France.
authors.forename: Fares
authors.initials: F
authors.lastname: Katranji
authors.affiliation: †Institute of Molecular Chemistry, University of Dijon, ICMUB CNRS, UMR6302 Dijon, France.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Chanteloup
authors.affiliation: †Institute of Molecular Chemistry, University of Dijon, ICMUB CNRS, UMR6302 Dijon, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Pirrotta
authors.affiliation: §Dimacell Imaging Ressource Center, UMR PAM, University of Burgundy, Agrosup, 21000 Dijon, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Perrier-Cornet
authors.affiliation: ‡Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
authors.forename: Judy M Y
authors.initials: JM
authors.lastname: Wong
authors.affiliation: †Institute of Molecular Chemistry, University of Dijon, ICMUB CNRS, UMR6302 Dijon, France.
authors.forename: David
authors.initials: D
authors.lastname: Monchaud
mesh_terms: D000818:Animals; D032701:Biomimetics; D002412:Cations; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry; D004247:DNA / Q000235:genetics*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D008024:Ligands; D061986:MCF-7 Cells; D008546:Melanoma, Experimental; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D017785:Photons; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D055672:Static Electricity
chemical_list: D002412:Cations; D002614:Chelating Agents; D005456:Fluorescent Dyes; D008024:Ligands; D012313:RNA; D004247:DNA