title: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production.
abstract: We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. clove@mit.edu.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Mozdzierz
authors.forename: Kerry R
authors.initials: KR
authors.lastname: Love
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Lee
authors.forename: Harry L T
authors.initials: HL
authors.lastname: Lee
authors.forename: Kartik A
authors.initials: KA
authors.lastname: Shah
authors.forename: Rajeev J
authors.initials: RJ
authors.lastname: Ram
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Love
mesh_terms: D019149:Bioreactors; D002478:Cells, Cultured; D019382:Human Growth Hormone / Q000096:biosynthesis*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D010477:Perfusion / Q000295:instrumentation; D010843:Pichia / Q000166:cytology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D019382:Human Growth Hormone