title: A case-control study of genotoxicity endpoints in patients with papillary thyroid cancer.
abstract: Thyroid cancer is one of the fastest growing cancer types worldwide. Using the cytokinesis-block micronucleus (CBMN) and comet assays, we performed a case-control study of 23 untreated papillary thyroid cancer (PTC) patients and 23 healthy volunteers. PTC patients showed higher basal DNA damage in peripheral blood lymphocytes. The CBMN assay indicated that the numbers of micronuclei, nuclear buds, and nucleoplasmic bridges among the cases were 2.67-, 2.79-, and 7.72-fold higher, respectively, than among the controls (p < 0.05). Comet assay tail lengths and tail intensities were 1.20- and 1.94-fold higher, respectively (p < 0.05). In additional, 14 thyroid tissues from PTC patients were probed for Raf-B and Ret expression; all samples were positive for at least one of these proteins.
authors.affiliation: Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
authors.forename: Marko
authors.initials: M
authors.lastname: Gerić
authors.affiliation: Department of Head and Neck Surgery, Clinical Hospital for Tumours, Zagreb, Croatia.
authors.forename: Renato
authors.initials: R
authors.lastname: Janušić
authors.affiliation: Department of Pathology, Clinical Hospital for Tumours, Zagreb, Croatia.
authors.forename: Božena
authors.initials: B
authors.lastname: Šarčević
authors.affiliation: Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia. Electronic address: vgaraj@imi.hr.
authors.forename: Vera
authors.initials: V
authors.lastname: Garaj-Vrhovac
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000097:blood / Q000235:genetics*; D002291:Carcinoma, Papillary; D016022:Case-Control Studies; D004249:DNA Damage; D005260:Female; D042822:Genomic Instability; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D010865:Pilot Projects; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000097:blood / Q000235:genetics*
chemical_list: D051096:Proto-Oncogene Proteins c-ret; C099282:RET protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf
keywords: Cancer prevention; Comet assay; Micronucleus test; Papillary thyroid cancer; Raf-B; Ret