title: Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae.
abstract: The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally.
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, W2 1PG, UK. Electronic address: j.bosse@imperial.ac.uk.
authors.forename: Janine T
authors.initials: JT
authors.lastname: Bossé
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, W2 1PG, UK.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, W2 1PG, UK.
authors.forename: Tom G
authors.initials: TG
authors.lastname: Atherton
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, W2 1PG, UK.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Walker
authors.affiliation: Animal and Plant Health Agency (APHA) Bury St Edmunds, Rougham Hill, Bury St Edmunds, Suffolk, IP33 2RX, UK.
authors.forename: Susanna M
authors.initials: SM
authors.lastname: Williamson
authors.affiliation: Animal and Plant Health Agency (APHA) Bury St Edmunds, Rougham Hill, Bury St Edmunds, Suffolk, IP33 2RX, UK.
authors.forename: Jon
authors.initials: J
authors.lastname: Rogers
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
authors.forename: Roy R
authors.initials: RR
authors.lastname: Chaudhuri
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
authors.forename: Lucy A
authors.initials: LA
authors.lastname: Weinert
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
authors.forename: Matthew T G
authors.initials: MT
authors.lastname: Holden
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: Maskell
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Tucker
authors.affiliation: Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, WC1E 7HT, UK.
authors.forename: Brendan W
authors.initials: BW
authors.lastname: Wren
authors.affiliation: Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, Hatfield, Hertfordshire, AL9 7TA, UK.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Rycroft
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, W2 1PG, UK. Electronic address: p.langford@imperial.ac.uk.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Langford
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology*; D016977:Actinobacillus pleuropneumoniae / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002702:Chloramphenicol Resistance / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D015661:Pasteurellaceae / Q000235:genetics* / Q000302:isolation & purification; D016871:Pasteurellaceae Infections / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C035534:florfenicol; D013839:Thiamphenicol