title: Monoclonal Antibody-Defined Specific C Epitope of Brucella O-Polysaccharide Revisited.
abstract: The C epitope of Brucella O-polysaccharide (O-PS) has so far lacked definitive structural identity. Revised structures for this antigen revealed a unique capping perosamine tetrasaccharide consisting of a sequence of 1,2:1,3:1,2 interresidue linkages. Here, using synthetic oligosaccharide glycoconjugates, the α-1,3 linkage of the O-PS is shown to be an integral structural requirement of this epitope. Although A-dominant strains possess only one or two copies of the capping tetrasaccharide, this creates a unique pentasaccharide antigenic determinant with the linkage sequence 1,2:1,3:1,2:1,2 that is always present in major pathogenic Brucella species.
authors.affiliation: INRA, UMR1282 Infectiologie et Santé Publique, Nouzilly, France Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, Tours, France michel.zygmunt@tours.inra.fr.
authors.forename: Michel S
authors.initials: MS
authors.lastname: Zygmunt
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: David R
authors.initials: DR
authors.lastname: Bundle
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: N Vijaya
authors.initials: NV
authors.lastname: Ganesh
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Guiard
authors.affiliation: INRA, UMR1282 Infectiologie et Santé Publique, Nouzilly, France Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, Tours, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Cloeckaert
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002002:Brucella / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D019081:O Antigens; D011135:Polysaccharides, Bacterial