title: Sensitive and selective determination of polycyclic aromatic hydrocarbons in mainstream cigarette smoke using a graphene-coated solid-phase microextraction fiber prior to GC/MS.
abstract: A simple method has been developed for the simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) in mainstream cigarette smoke. The procedure is based on employing a homemade graphene-coated solid-phase microextraction (SPME) fiber for extraction prior to GC/MS. In comparison to commercial 100-μm poly(dimethyl siloxane) (PDMS) fiber, the graphene-coated SPME fiber exhibits advantageous cleanup and preconcentration efficiencies. By collecting the particulate phase 5 cigarettes, the LODs and LOQs of 16 target PAHs were 0.02-0.07 and 0.07-0.22 ng/cigarette, respectively, and all of the linear correlation efficiencies were larger than 0.995. The validation results also indicate that the method has good repeatability (RSD between 4.2% and 9.5%) and accuracy (spiked recoveries between 80% and 110%). The developed method was applied to analyze two Kentucky reference cigarettes (1R5F and 3R4F) and six Chinese brands of cigarettes. In addition, the PAH concentrations in the particulate phase of the smoke from the 1R5F Kentucky cigarettes were in good agreement with recently reported results. Due to easy operation and good validation results, this SPME-GC/MS method may be an excellent alternative for trace analysis of PAHs in cigarette smoke.
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China. Electronic address: wangxiaoyu@iccas.ac.cn.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
mesh_terms: D004867:Equipment Design; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D015203:Reproducibility of Results; D012906:Smoke / Q000032:analysis*; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012906:Smoke; D006108:Graphite
keywords: Graphene; Mainstream cigarette smoke; Polycyclic aromatic hydrocarbons; Solid phase micro-extraction