title: A proteomic study of the differential protein expression in MDBK cells after bovine herpesvirus type 1 infection (BHV-1) strain treatment.
abstract: Different BHV-1 strains, such as the virulent IBRV LN01/08 strains and the attenuated vaccine strain IBRV LNM, produces different clinical immune responses; however, the study of the differential protein expression in Madin-Darby bovine kidney (MDBK) cells after BHV-1-infection still remains unclear. Here, we applied a comparative proteomic strategy, based on 2D and MALDI-TOF/MS platforms, to examine the differential expression of proteins in MDBK cells that were treated and not treated with virulent IBRV LN01/08 and attenuated IBRV LNM strains. A total of eight differential proteins, including pyruvate kinase, heat shock protein (HSP) 90 (HSP90AA1 and HSP90AB1), annexin A, albumin (ALB), scinderin (SCIN), tubulin (alpha 1a) and vimentin (VIM), were identified. Among these proteins, pyruvate kinase, and HSP90 (HSP90AB1), tubulin and vimentin were identified in the virulent IBRV LN01/08 strain group, but were not identified in the attenuated IBRV LNM group. These results play an important role in tumor formation and development, cell migration, tumor cell line apoptosis, cell invasion and viral infection. The HSP90 (HSP90AA1) protein was identified in the control group and the attenuated IBRV LNM-infected group. Most studies have shown that HSP90 proteins were more of a cancer gene target, and inhibiting its function would result to oncogene degradation during cancer treatment. On the other hand, ALB is associated to cell differentiation, apoptosis, necrosis, cell death, viral infection, autophagy, interstitial tissue inflammation, and cell survival. These results provide a theoretical basis for the systematic understanding of BHV-1-infection mechanisms and BHV-1-induced immune responses.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences Changchun 130112, Jilin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences Changchun 130112, Jilin, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences 666 West Liuying Road, Changchun 130112, Jilin, China.
authors.forename: Linna
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha 410125, Hunan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences Changchun 130112, Jilin, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wen
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences Changchun 130112, Jilin, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences Changchun 130112, Jilin, China.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
keywords: BHV-1; MDBK; comparative proteome