title: Molecular phylogeny of anoplocephalid tapeworms (Cestoda: Anoplocephalidae) infecting humans and non-human primates.
abstract: Anoplocephalid tapeworms of the genus Bertiella Stiles and Hassall, 1902 and Anoplocephala Blanchard, 1848, found in the Asian, African and American non-human primates are presumed to sporadic ape-to-man transmissions. Variable nuclear (5.8S-ITS2; 28S rRNA) and mitochondrial genes (cox1; nad1) of isolates of anoplocephalids originating from different primates (Callicebus oenanthe, Gorilla beringei, Gorilla gorilla, Pan troglodytes and Pongo abelii) and humans from various regions (South America, Africa, South-East Asia) were sequenced. In most analyses, Bertiella formed a monophyletic group within the subfamily Anoplocephalinae, however, the 28S rRNA sequence-based analysis indicated paraphyletic relationship between Bertiella from primates and Australian marsupials and rodents, which should thus be regarded as different taxa. Moreover, isolate determined as Anoplocephala cf. gorillae from mountain gorilla clustered within the Bertiella clade from primates. This either indicates that A. gorillae deserves to be included into the genus Bertiella, or, that an unknown Bertiella species infects also mountain gorillas. The analyses allowed the genetic differentiation of the isolates, albeit with no obvious geographical or host-related patterns. The unexpected genetic diversity of the isolates studied suggests the existence of several Bertiella species in primates and human and calls for revision of the whole group, based both on molecular and morphological data.
authors.affiliation: Department of Physiology,Faculty of Veterinary Medicine,University of Veterinary and Pharmaceutical Sciences,Palackého tř.1/3,612 00 Brno,Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Doležalová
authors.affiliation: Department of Pathology and Parasitology,Faculty of Veterinary Medicine,University of Veterinary and Pharmaceutical Sciences,Palackého tř.1/3,612 00 Brno,Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Vallo
authors.affiliation: Department of Pathology and Parasitology,Faculty of Veterinary Medicine,University of Veterinary and Pharmaceutical Sciences,Palackého tř.1/3,612 00 Brno,Czech Republic.
authors.forename: Klára J
authors.initials: KJ
authors.lastname: Petrželková
authors.affiliation: Department of Botany and Zoology,Faculty of Science,Masaryk University,Kotlářská 267/2,611 37 Brno,Czech Republic.
authors.forename: Ivona
authors.initials: I
authors.lastname: Foitová
authors.affiliation: Department of Parasitology,Faculty of Veterinary Medicine,Gadjah Mada University,Yogyakarta,Indonesia.
authors.forename: Wisnu
authors.initials: W
authors.lastname: Nurcahyo
authors.affiliation: Rwanda Development Board,Gishushu,Nyarutarama Road,P.O. Box 6239 Kigali,Rwanda.
authors.forename: Antoine
authors.initials: A
authors.lastname: Mudakikwa
authors.affiliation: Primate Research Institute,Kyoto University,Inuyama,Aichi 484-8506,Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Hashimoto
authors.affiliation: Institute of Parasitology, Biology Centre, Czech Academy of Sciences,370 05 České Budějovice,Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Jirků
authors.affiliation: Institute of Parasitology, Biology Centre, Czech Academy of Sciences,370 05 České Budějovice,Czech Republic.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
authors.affiliation: Institute of Parasitology, Biology Centre, Czech Academy of Sciences,370 05 České Budějovice,Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Scholz
authors.affiliation: Department of Pathology and Parasitology,Faculty of Veterinary Medicine,University of Veterinary and Pharmaceutical Sciences,Palackého tř.1/3,612 00 Brno,Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Modrý
mesh_terms: D000349:Africa; D000818:Animals; D001210:Asia, Southeastern; D002589:Cestoda / Q000145:classification* / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D050259:Genes, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D010802:Phylogeny; D011323:Primates / Q000469:parasitology*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D013020:South America; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S
keywords: Anoplocephala; Bertiella; phylogeny; primates; zoonotic potential