title: Simple and rapid determination of unsaturated fatty acids in 1 µl of rat plasma by LC-MS/MS.
abstract: BACKGROUND
  Deficiency or imbalance of unsaturated fatty acids will promote the pathogenesis of many diseases. In order to monitor the exposure of unsaturated fatty acids, the method based on LC-MS/MS was developed.
  
  
  RESULTS
  Standard calibration curves for α-linolenic acid, linoleic acid, palmitoleic acid and oleic acid were linear (r ≥0.99). The intra-and interbatch accuracy (RE%) ranged from -4.5 to 8.6%, while the intra- and interbatch precisions (RSD%) were ≤8.7%. The extraction recovery varied from 85.4 to 99.6%, and no obvious matrix effect was observed.
  
  
  CONCLUSIONS
  The method offers a simple approach for measuring 4 unsaturated fatty acids in 1 μl rat plasma within 3.95 min.
authors.forename: Shirui
authors.initials: S
authors.lastname: Zheng
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Ma
authors.forename: Feifeng
authors.initials: F
authors.lastname: Song
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Ye
authors.forename: Ruwei
authors.initials: R
authors.lastname: Wang
authors.forename: Meijuan
authors.initials: M
authors.lastname: Tu
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
authors.forename: Huidi
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000097:blood* / Q000302:isolation & purification; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D005231:Fatty Acids, Unsaturated