title: Endogenous Estrogen-Mediated Heme Oxygenase Regulation in Experimental Menopause.
abstract: Estrogen deficiency is one of the main causes of age-associated diseases in the cardiovascular system. Female Wistar rats were divided into four experimental groups: pharmacologically ovariectomized, surgically ovariectomized, and 24-month-old intact aging animals were compared with a control group. The activity and expression of heme oxygenases (HO) in the cardiac left ventricle, the concentrations of cardiac interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), the myeloperoxidase (MPO) activity in the cardiac left ventricle, and the effects of heme oxygenase blockade (by 24-hour and 1-hour pretreatment with tin-protoporphyrin IX, SnPP) on the epinephrine and phentolamine-induced electrocardiogram ST segment changes in vivo were investigated. The cardiac HO activity and the expression of HO-1 and HO-2 were significantly decreased in the aged rats and after ovariectomy. Estrogen depletion was accompanied by significant increases in the expression of IL-6 and TNF-α. The aged and ovariectomized animals exhibited a significantly elevated MPO activity and a significant ST segment depression. After pretreatment with SnPP augmented ST segment changes were determined. These findings demonstrate that the sensitivity to cardiac ischemia in estrogen depletion models is associated with suppression of the activity and expression of the HO system and increases in the secretion of proinflammatory cytokines and biomarkers.
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Anikó
authors.initials: A
authors.lastname: Pósa
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Renáta
authors.identifier: 0000-0001-7881-2082
authors.initials: R
authors.lastname: Szabó
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Anett
authors.identifier: 0000-0002-9365-9837
authors.initials: A
authors.lastname: Csonka
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Médea
authors.initials: M
authors.lastname: Veszelka
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Anikó Magyariné
authors.initials: AM
authors.lastname: Berkó
authors.affiliation: Faculty of Dentistry and Department of Orthodontics and Pediatric Dentistry, University of Szeged, Szeged 6720, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Baráth
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Ménesi
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Pávó
authors.affiliation: Department of Cardiology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Mariann
authors.identifier: 0000-0002-7083-2107
authors.initials: M
authors.lastname: Gyöngyösi
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: László
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Krisztina
authors.identifier: 0000-0002-0644-1718
authors.initials: K
authors.lastname: Kupai
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Varga
mesh_terms: D000818:Animals; D004562:Electrocardiography; D004837:Epinephrine / Q000633:toxicity; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006331:Heart Diseases / Q000209:etiology; D006352:Heart Ventricles / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008593:Menopause; D008665:Metalloporphyrins / Q000737:chemistry; D010052:Ovariectomy; D009195:Peroxidase / Q000378:metabolism; D010646:Phentolamine / Q000633:toxicity; D011524:Protoporphyrins / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004967:Estrogens; D015850:Interleukin-6; D008665:Metalloporphyrins; D011524:Protoporphyrins; D014409:Tumor Necrosis Factor-alpha; C032628:tin protoporphyrin IX; D009195:Peroxidase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D004837:Epinephrine; D010646:Phentolamine