title: Non-extended cryoablation could be a new strategy in lung cancer management: An experiment on green fluorescent protein-labeled Lewis lung cancer-bearing mice.
abstract: Modern cryoablation has been performed in solid tumor management for more than two decades. Following the surgical spirits, it seems natural to pursue radical procedures in clinical practice, which results in unnecessary adverse effects. The attempt to use non-extended procedure made some marked achievements in practice but was criticized severely, because it was supposed to induce residual tumors, which would trigger the rapid development of cancer. Oncologists favored this procedure, however, claiming that non-extended cryoablation let lung cancer patients have higher quality of lives and longer survivals, in light of clinical observations. Therefore, this study was conducted trying to solve this controversy. In this study, fifty female C57BL/6J mice were grafted green fluorescent protein (GFP)-labeled Lewis lung cancer and randomized into two groups. The bidirectional diameters and fluorescence intensity of tumors, and the body weight of mice were recorded. Two weeks after the intervention, tumor volumes increased 20.95% in the cryoablation group, significantly different from that in the control group; the fluorescence intensity decreased 49.85% in the cryoablation group but increased 125.07% in the control group. Lung metastases could be observed in only 20% of mice in the cryosurgery group, contrasted to 64% in the control group. The non-extended lung cancer cryoablation does induce marginal tumor residuals, but will not trigger rapid tumor development. Inversely, the residual tumor cells are severely struck and the metastases are suppressed after the intervention. It could be a new strategy in lung cancer management, even for patients not in early stage.
authors.affiliation: Oncology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6 Fangxingyuan, No. 1 Community, Fengtai District, Beijing, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Oncology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6 Fangxingyuan, No. 1 Community, Fengtai District, Beijing, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Health-cultivation Laboratory of the Ministry of Education, Beijing University of Chinese Medicine, No. 11 Beisanhuan East Road, Chaoyang District, Beijing, China.
authors.forename: Linyi
authors.initials: L
authors.lastname: Li
authors.affiliation: Animal Experiment Laboratory, AntiCancer Biotech (Beijing) Co., Ltd., No. 11 Huatong Road, Changping district, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qi
authors.affiliation: Animal Experiment Laboratory, AntiCancer Biotech (Beijing) Co., Ltd., No. 11 Huatong Road, Changping district, Beijing, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Shi
authors.affiliation: Animal Experiment Laboratory, AntiCancer Biotech (Beijing) Co., Ltd., No. 11 Huatong Road, Changping district, Beijing, China.
authors.forename: Chongyi
authors.initials: C
authors.lastname: Lu
authors.affiliation: Research and Development Department, Hygea Medical Technology Co., Ltd., Block C, SP Tower, Tsinghua Science Park, Beijing, China.
authors.forename: Sida
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Research and Development Department, Hygea Medical Technology Co., Ltd., Block C, SP Tower, Tsinghua Science Park, Beijing, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Geng
authors.affiliation: Animal Experiment Laboratory, AntiCancer Biotech (Beijing) Co., Ltd., No. 11 Huatong Road, Changping district, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Oncology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6 Fangxingyuan, No. 1 Community, Fengtai District, Beijing, China.
authors.forename: Quanwang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Oncology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6 Fangxingyuan, No. 1 Community, Fengtai District, Beijing, China. Electronic address: drkaiwenhu@126.com.
authors.forename: Kaiwen
authors.initials: K
authors.lastname: Hu
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000601:surgery*; D045744:Cell Line, Tumor; D003452:Cryosurgery / Q000379:methods*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000601:surgery; D008175:Lung Neoplasms / Q000556:secondary / Q000601:surgery*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D011897:Random Allocation; D016896:Treatment Outcome
chemical_list: D049452:Green Fluorescent Proteins
keywords: Cryoablation method; Efficacy; Green fluorescent protein-labeled; Green therapy; Lewis lung cancer; Lung cancer; Non-extended cryoablation