title: Antiproliferative effects and molecular mechanisms of troglitazone in human cervical cancer in vitro.
abstract: We investigated the effects of troglitazone on human cervical cancer SiHa cells and its mechanisms of action. SiHa cells were incubated with different concentrations of troglitazone (100, 200, or 400 μg/mL) for 24, 48, and 72 hours. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay; cell cycle and apoptosis were detected by flow cytometry; and morphology of SiHa cells was observed under an inverted microscope. pcDNA3.1 and pcDNA3.1-Skp2 plasmids were constructed and then transfected into SiHa cells. Protein expression was analyzed by Western blotting. Troglitazone inhibited the proliferation of SiHa cells in a time- and concentration-dependent manner. Troglitazone caused G0/1 phase arrest but failed to reduce apoptosis in SiHa cells. Troglitazone significantly increased expression of p27 but decreased Skp2 expression. Skp2 overexpression inhibited the role of troglitazone in increasing expression of p27, and the cell cycle inhibitory effect of troglitazone. Troglitazone can inhibit SiHa cell viability by affecting cell cycle distribution but not apoptosis, and Skp2 and p27 may play a critical role.
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Chemotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Guoren
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Hanzi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Emei
authors.initials: E
authors.lastname: Lu
authors.affiliation: Department of Radiotherapy, Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: He
keywords: SiHa cells; apoptosis; cell cycle