title: A composite method for mapping quantitative trait loci without interference of female achiasmatic and gender effects in silkworm, Bombyx mori.
abstract: The silkworm, Bombyx mori, is an economically important insect that was domesticated more than 5000 years ago. Its major economic traits focused on by breeders are quantitative traits, and an accurate and efficient QTL mapping method is necessary to explore their genetic architecture. However, current widely used QTL mapping models are not well suited for silkworm because they ignore female achiasmate and gender effects. In this study, we propose a composite method combining rational population selection and special mapping methods to map QTL in silkworm. By determining QTL for cocoon shell weight (CSW), we demonstrated the effectiveness of this method. In the CSW mapping process, only 56 markers were used and five loci or chromosomes were detected, more than in previous studies. Additionally, loci on chromosomes 1 and 11 dominated and accounted for 35.10% and 15.03% of the phenotypic variance respectively. Unlike previous studies, epistasis was detected between loci on chromosomes 11 and 22. These mapping results demonstrate the power and convenience of this method for QTL mapping in silkworm, and this method may inspire the development of similar approaches for other species with special genetic characteristics.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: W
authors.initials: W
authors.lastname: Zuo
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: X
authors.initials: X
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: H
authors.initials: H
authors.lastname: Hu
authors.affiliation: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China.
authors.forename: L
authors.initials: L
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: J
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: G
authors.initials: G
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: R
authors.initials: R
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: F
authors.initials: F
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqi-ng, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
authors.forename: C
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D004843:Epistasis, Genetic; D005260:Female; D005838:Genotype; D008297:Male; D010641:Phenotype; D011679:Pupa; D040641:Quantitative Trait Loci; D047011:Silk
chemical_list: D047011:Silk
keywords: Bombyx mori; cocoon shell weight; genetic architecture; population selection; quantitative trait loci mapping