title: Quercus Suber L. Cork Extracts Induce Apoptosis in Human Myeloid Leukaemia HL-60 Cells.
abstract: Quercus suber L. cork contains a diversity of phenolic compounds, mostly low molecular weight phenols. A rising number of reports support with convergent findings that polyphenols evoke pro-apoptotic events in cancerous cells. However, the literature related to the anti-cancer bioactivity of Q. suber L. cork extractives (QSE) is still limited. Herein, we aim to describe the antitumor potential displayed by cork extractives obtained by different extraction methods in the human promyelocytic leukaemia cells. In order to quantify the effects of QSE on cancer cells viability, phosphatidylserine exposure, caspase-3 activity, mitochondrial membrane potential and cell cycle were evaluated. The results indicated that the QSE present a time-dependent and dose-dependent cytotoxicity in the human promyelocytic leukaemia cells. Such a noxious effect leads these leukaemia cells to their death through apoptotic processes by altering the mitochondrial outer membrane potential, activating caspase-3 and externalizing phosphatidylserine. However, cells cycle progression was not affected by the treatments. This study contributes to open a new way to use this natural resource by exploiting its anti-cancer properties. Moreover, it opens new possibilities of application of cork by-products, being more efficient in the sector of cork-based agriculture. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Physiology, University of Extremadura, Badajoz, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Bejarano
authors.affiliation: Institute of Cork, Wood and Charcoal. Centre for Scientific Research and Technology in Extremadura (CICYTEX), Government of Extremadura, Mérida, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Godoy-Cancho
authors.affiliation: Department of Physiology, University of Extremadura, Badajoz, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Franco
authors.affiliation: Institute of Cork, Wood and Charcoal. Centre for Scientific Research and Technology in Extremadura (CICYTEX), Government of Extremadura, Mérida, Spain.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Martínez-Cañas
authors.affiliation: Department of Physiology, University of Extremadura, Badajoz, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Tormo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010718:Phosphatidylserines / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D029963:Quercus / Q000737:chemistry*
chemical_list: D010636:Phenols; D010718:Phosphatidylserines; D010936:Plant Extracts; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: apoptosis; cancer cells; cork extracts; polyphenols