title: Infectious Bursal Disease Virus non-structural protein VP5 is not a transmembrane protein.
abstract: Infectious Bursal Disease Virus (IBDV) causes a highly relevant poultry disease that affects young chickens causing, among other effects, immunosuppression. IBDV is a bi-segmented double stranded RNA virus. The smaller ORF of larger RNA segment encodes VP5, a 17-kDa non-structural protein. Although it is an important protein for viral replication cycle, the definition of its specific role and subcellular localization remains unclear. In the present work we demonstrate, using imaging techniques, that VP5 is not a type II transmembrane protein but an intracellular membrane-associated protein. This finding might provide evidences of VP5 interaction with cellular proteins and its functions.
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Ciudad de Buenos Aires, Argentina.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Carballeda
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Ciudad de Buenos Aires, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Buenos Aires, Argentina.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Maroniche
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Ciudad de Buenos Aires, Argentina.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Lucero
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina.
authors.forename: Matías
authors.initials: M
authors.lastname: Richetta
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Ciudad de Buenos Aires, Argentina.
authors.forename: Evangelina
authors.initials: E
authors.lastname: Gómez
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Ciudad de Buenos Aires, Argentina.
authors.forename: Silvina
authors.initials: S
authors.lastname: Chimeno Zoth
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, Cc 25, B1712WAA, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Ciudad de Buenos Aires, Argentina. Electronic address: berinstein.analia@inta.gob.ar.
authors.forename: Analía
authors.initials: A
authors.lastname: Berinstein
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D007243:Infectious bursal disease virus / Q000502:physiology*; D011784:Quail; D017361:Viral Nonstructural Proteins / Q000032:analysis*
chemical_list: C518635:VP5 protein, infectious bursal disease virus; D017361:Viral Nonstructural Proteins
keywords: Infectious Bursal Disease Virus; Membrane topology; Protein localization; VP5