title: Treatment by Therapeutic Magnetic Resonance (TMR™) increases fibroblastic activity and keratinocyte differentiation in an in vitro model of 3D artificial skin.
abstract: This study investigated the effect of extremely low-frequency pulsed electromagnetic fields (PEMFs) on skin wound healing in an in vitro dermal-like tissue. In this study, fibroblast and endothelial cells were utilized for the in vitro reconstruction of dermal-like tissues treated for various times up to 21 days with PEMFs. The effects of PEMFs on cell proliferation (MTT test), cell ageing (β-galactosidase test, ROS production), gene expression, the quality of the extracellular matrix and the amount of fibroblast growth factors were analysed. The high quality of the dermis products in the presence of PEMFs at the end of the study was confirmed through the high degree of organization of keratinocytes, which were subsequently seeded on the aforementioned in vitro reconstructed dermis. The cells organized themselves in well-defined multi-layers and were better organized compared with the epidermis present on the dermis that was obtained without PEMF treatment. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Ferroni
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: Gloria
authors.initials: G
authors.lastname: Bellin
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Emer
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Rizzuto
authors.affiliation: Dipartimenti di Medicina Animale, Produzioni e Salute (MAPS), University of Padova, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Isola
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gardin
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D017768:Skin, Artificial
keywords: artificial skin; electromagnetic field; fibroblasts; keratinocytes; wound healing