title: Comparison of four commercial viral load techniques in an area of non-B HIV-1 subtypes circulation.
abstract: The aim of this study was to compare four HIV-1 viral quantitation platforms, Nuclisens EasyQ v2.0(®) (EQ), COBAS AmpliPreP/Cobas Taqman(®) HIV-1 test v 2.0 (CTM), GENERIC HIV CHARGE VIRALE(®) (GEN), with Abbott Real Time HIV-1(®) (m2000sp/rt) as reference technique. The study had first evaluated m2000sp/rt performances and then compared quantitation between techniques. Discordant samples were genotyped on gag and pol gene and sequences were analyzed using Sequence locator and SeqPublish to detect eventual mismatches. Performance analysis of m2000sp/rt showed good results with coefficients of variation values (CV) of 1.35%, 0.65%, and 0.54% for repeatability testing of low, intermediate and high concentrations, respectively. Reproducibility tests showed low CV values with 2.36% and 1.42% for low and high concentration levels, respectively and contamination test was very low value with 0.94%. Correlation and concordance between techniques ranged from r(2)=0.98 and bias=-0.00185 (for m2000sp/rt vs CTM) to r(2)=0.90 and bias=-0.135 (for EQ vs GEN). Discrepancies were observed on 37 samples mostly CRF02_AG but despite some mismatches, sequence analysis (26/37) did not show any remarkable differences between CRF02_AG queries and references. This study showed good correlation and good concordance between techniques. However, EQ yielded under-quantitation of CRF02_AG.
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Ousseynou
authors.initials: O
authors.lastname: Ndiaye
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal. Electronic address: halimatoudiop@yahoo.fr.
authors.forename: Halimatou
authors.initials: H
authors.lastname: Diop-Ndiaye
authors.affiliation: Université de Ouagadougou, Service de Bactériologie-Virologie, CHU Souro Sanou, Bobo Dioulasso, Burkina Faso.
authors.forename: Abdou Salam
authors.initials: AS
authors.lastname: Ouedraogo
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Fatim Zahra
authors.initials: FZ
authors.lastname: Fall-Malick
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Amina
authors.initials: A
authors.lastname: Sow-Sall
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Moussa
authors.initials: M
authors.lastname: Thiam
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Abou Abadallah Malick
authors.initials: AA
authors.lastname: Diouara
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Cheikh Tidiane
authors.initials: CT
authors.lastname: Ndour
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Aïssatou
authors.initials: A
authors.lastname: Gaye-Diallo
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Souleymane
authors.initials: S
authors.lastname: Mboup
authors.affiliation: Université Cheikh Anta Diop de Dakar, Laboratoire de Bactériologie-Virologie CHU A. le Dantec, Dakar, Senegal.
authors.forename: Coumba
authors.initials: C
authors.lastname: Toure-Kane
mesh_terms: D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D019562:Viral Load / Q000379:methods*; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus
chemical_list: D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus
keywords: Comparison; HIV-1; Viral load