title: T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells.
abstract: The ELISPOT is a powerful functional assay used to detect biological activity and immunological secretions from immune cells. In this chapter, we specifically discuss T cell ELISPOT methods for the detection of secreted cytokines. A detailed protocol is given enabling the detection of interferon gamma-secreting CD8(+) T cells and/or peripheral blood mononuclear cells following their isolation and polyclonal activation. Included is a brief discussion on choosing the activation method for your T cell ELISPOT assay, as well as additional instructions for the adaptation of this protocol for the study of memory and antigen-specific T cell responses.
authors.affiliation: Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, USA.
authors.forename: Kerry M
authors.initials: KM
authors.lastname: Leehan
authors.forename: Kristi A
authors.initials: KA
authors.lastname: Koelsch
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002469:Cell Separation; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*
chemical_list: D007371:Interferon-gamma