title: miR-217 targeting Wnt5a in osteosarcoma functions as a potential tumor suppressor.
abstract: Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults, however roles of microRNAs (miRNAs) in OS is still unclear. miR-217 is recently widely studied in various cancers, but not including OS. This study is aimed to investigate the expression and cellular function of miR-217 in OS. The data showed that miR-217 expression was consistently lower in OS tissues and cell lines than the normal controls. Restoration of miR-217 expression in MG-63 and U2OS cells could inhibit cell proliferation, migration and invasion, and induced apoptosis. Bioinformatic prediction suggested that Wnt5a is a target gene of miR-217. Using luciferase assay, mRNA and protein expression analysis, it was verified that Wnt5a was a target gene of miR-217 in OS cells. Restored expression of Wnt5a weakened miR-217-mediated suppression of tumor progression. Taken together, our data indicate that miR-217 functions as a tumor suppressor in OS by suppressing Wnt5a expression.
authors.affiliation: Department of Orthopaedic, Zhongnan Hospital of Wuhan University, 169, Donghu Road, Wuhan, Hubei 430071, China. Electronic address: renxiong_wei@126.com.
authors.forename: Renxiong
authors.initials: R
authors.lastname: Wei
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Zhouming
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Radiology, Hubei Cancer Hospital, Wuhan, Hubei, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Su
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism; D000071818:Wnt-5a Protein
chemical_list: C553315:MIRN217 microRNA, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
keywords: Osteosarcoma; Tumor progression; Wnt5a; miR-217