title: Development of a Model of Chronic Kidney Disease in the C57BL/6 Mouse with Properties of Progressive Human CKD.
abstract: Chronic kidney disease (CKD) is a major healthcare problem with increasing prevalence in the population. CKD leads to end stage renal disease and increases the risk of cardiovascular disease. As such, it is important to study the mechanisms underlying CKD progression. To this end, an animal model was developed to allow the testing of new treatment strategies or molecular targets for CKD prevention. Many underlying risk factors result in CKD but the disease itself has common features, including renal interstitial fibrosis, tubular epithelial cell loss through apoptosis, glomerular damage, and renal inflammation. Further, CKD shows differences in prevalence between the genders with premenopausal women being relatively resistant to CKD. We sought to develop and characterize an animal model with these common features of human CKD in the C57BL/6 mouse. Mice of this genetic background have been used to produce transgenic strains that are commercially available. Thus, a CKD model in this strain would allow the testing of the effects of numerous genes on the severity or progression of CKD with minimal cost. This paper describes such a mouse model of CKD utilizing angiotensin II and deoxycorticosterone acetate as inducers.
authors.affiliation: Department of Medicine, Division of Nephrology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Zahraa
authors.identifier: 0000-0001-8436-0553
authors.initials: Z
authors.lastname: Mohammed-Ali
authors.affiliation: Department of Medicine, Division of Nephrology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Gaile L
authors.initials: GL
authors.lastname: Cruz
authors.affiliation: Department of Medicine, Division of Nephrology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Medicine, Division of Nephrology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Carlisle
authors.affiliation: Department of Medicine, Division of Nephrology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Kaitlyn E
authors.initials: KE
authors.lastname: Werner
authors.affiliation: Department of Medicine, Division of Respirology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Kjetil
authors.initials: K
authors.lastname: Ask
authors.affiliation: Department of Medicine, Division of Nephrology, McMaster University and St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada L8N 4A6.
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Dickhout
mesh_terms: D000804:Angiotensin II / Q000008:administration & dosage; D000818:Animals; D064791:Desoxycorticosterone Acetate / Q000008:administration & dosage; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D051436:Renal Insufficiency, Chronic / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology*
chemical_list: D000804:Angiotensin II; D064791:Desoxycorticosterone Acetate