title: Purification, crystallization and X-ray crystallographic studies of a Bacillus cereus MepR-like transcription factor, BC0657.
abstract: Transcription factors of the MarR family respond to internal and external changes and regulate a variety of biological functions through ligand association with microorganisms. MepR belongs to the MarR family, and its mutations are associated with the development of multidrug resistance in Staphylococcus aureus, which has caused a growing health problem. In this study, a Bacillus cereus MepR-like transcription regulator, BC0657, was crystallized. The BC0657 crystals diffracted to 2.05 Å resolution and belonged to either space group P6(2)22 or P6(4)22, with unit-cell parameters a = 110.57, b = 110.57, c = 67.29 Å. There was one molecule per asymmetric unit. Future comparative structural studies on BC0657 would extend knowledge of ligand-induced transcriptional regulatory mechanisms in the MarR family and would make a significant contribution to the design of antibiotic drugs against multidrug-resistant bacteria.
authors.affiliation: Division of Biological Science and Technology, Yonsei University, 1 Yonseidae-gil, Wonju-si, Ganwon-do 220-710, Republic of Korea.
authors.forename: Min Uk
authors.initials: MU
authors.lastname: Cho
authors.affiliation: Division of Biological Science and Technology, Yonsei University, 1 Yonseidae-gil, Wonju-si, Ganwon-do 220-710, Republic of Korea.
authors.forename: Meong Il
authors.initials: MI
authors.lastname: Kim
authors.affiliation: Division of Biological Science and Technology, Yonsei University, 1 Yonseidae-gil, Wonju-si, Ganwon-do 220-710, Republic of Korea.
authors.forename: Minsun
authors.initials: M
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors
keywords: Bacillus cereus; MepR; transcription factor