title: Bacterial sensing underlies artificial sweetener-induced growth of gut Lactobacillus.
abstract: Disruption in stable establishment of commensal gut microbiota by early weaning is an important factor in susceptibility of young animals to enteric disorders. The artificial sweetener SUCRAM [consisting of neohesperidin dihydrochalcone (NHDC) and saccharin] included in piglets' feed reduces incidence of enteric disease. Pyrosequencing of pig caecal 16S rRNA gene amplicons identified 25 major families encompassing seven bacterial classes with Bacteroidia, Clostridia and Bacilli dominating the microbiota. There were significant shifts in microbial composition in pigs maintained on a diet containing SUCRAM, establishing SUCRAM as a major influence driving bacterial community dynamics. The most notable change was a significant increase of Lactobacillaceae population abundance, almost entirely due to a single phylotype, designated Lactobacillus 4228. The sweetener-induced increase in Lactobacillaceae was observed in two different breeds of pigs signifying a general effect. We isolated Lactobacillus 4228, sequenced its genome and found it to be related to Lactobacillus amylovorus. In vitro analyses of Lactobacillus 4228 growth characteristics showed that presence of NHDC significantly reduces the lag phase of growth and enhances expression of specific sugar transporters, independently of NHDC metabolism. This study suggests that sensing of NHDC by a bacterial plasma membrane receptor underlies sweetener-induced growth of a health promoting gut bacterium.
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Kristian
authors.initials: K
authors.lastname: Daly
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Alistair C
authors.initials: AC
authors.lastname: Darby
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Hall
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Wilkinson
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Pisut
authors.initials: P
authors.lastname: Pongchaikul
authors.affiliation: InVivo ANH, Saint Nolff, France.
authors.forename: David
authors.initials: D
authors.lastname: Bravo
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Soraya P
authors.initials: SP
authors.lastname: Shirazi-Beechey
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002432:Cecum / Q000378:metabolism / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D012439:Saccharin / Q000378:metabolism; D013549:Sweetening Agents / Q000378:metabolism*; D013552:Swine / Q000378:metabolism / Q000382:microbiology*; D014886:Weaning
chemical_list: D013549:Sweetening Agents; D012439:Saccharin