title: Expression of HAX-1 in colorectal cancer and its role in cancer cell growth.
abstract: Colorectal cancer (CRC) is one of the most common types of cancer worldwide. Hematopoietic cell‑specific protein 1‑associated protein X‑1 (HAX‑1) has been found to be involved in several types of cancer. However, the role of HAX‑1 in CRC remains to be elucidated. The aim of the present study was to investigate whether the expression of HAX‑1 is associated with the progression of CRC, and to determine the effects of HAX‑1 on the apoptosis and proliferation of CRC cells. Tumor tissues and adjacent noncancerous tissues were collected from 60 patients with CRC, following the provision of informed consent. The expression levels of HAX‑1 and the association with clinical and pathological characteristics were then analyzed. The expression levels of HAX‑1 were significantly higher in the cancerous tissues from the patients with CRC, particularly in tissues of an advanced stage of cancer. In addition, HAX‑1 expression was associated with malignant progression and poor prognosis. Furthermore, SW480 CRC cells, overexpressing HAX‑1, exhibited increased resistance to camptothecin in vitro, and promoted proliferation in vitro and in vivo. By contrast, HAX‑1 knockdown significantly decreased the proliferation. In addition, the expression levels of ki‑67 and phosphorylated‑akt were inhibited following HAX‑1 knockdown. In conclusion, the expression levels of HAX‑1 were increased in cancerous tissue from patients with CRC, and were associated with progression of the disease. These results suggested that HAX‑1 may contribute to chemotherapy resistance and malignant progression in CRC.
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Xiangshang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Fayong
authors.initials: F
authors.lastname: Hu
authors.affiliation: Experimental Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Anding
authors.initials: A
authors.lastname: Liu
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Deding
authors.initials: D
authors.lastname: Tao
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Leng
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Hu
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Gong
authors.affiliation: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
authors.forename: Xuelai
authors.initials: X
authors.lastname: Luo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000368:Aged; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C104759:HAX1 protein, human; D019394:Ki-67 Antigen; D034741:RNA, Small Interfering