title: Detection of porcine circovirus type 2 and its association with PMWS in wild boars and domestic pigs in Germany: a histopathological, immunohistochemical and molecular biological study.
abstract: Beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (PMWS). For the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in Germany by histopathology, immunohistochemistry (IHC) and quantitative PCR (qPCR). Whereas 102/340 domestic pigs were immunoreactive for PCV2 antigen in at least one examined tissue, only 8/356 wild boars reacted positively. Similar findings could be found in qPCR: all domestic pigs showed viral DNA in at least one tissue, while in the examined tissues of 170 wild boars PCV2-DNA was not detectable. The specimens were examined histologically for histiocytosis and depletion of lymphocytes, both typical for PMWS. Based on these findings, six wild boars and 69 domestic pigs were assumed to be affected by PMWS.
authors.forename: Corinna
authors.initials: C
authors.lastname: Hohloch
authors.forename: Gerald
authors.initials: G
authors.lastname: Reiner
authors.forename: Bastian
authors.initials: B
authors.lastname: Bronnert
authors.forename: Hermann
authors.initials: H
authors.lastname: Willems
authors.forename: Manfred
authors.initials: M
authors.lastname: Reinacher
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics; D005858:Germany; D015614:Histiocytosis; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D034421:Sus scrofa; D013552:Swine
chemical_list: D004279:DNA, Viral