title: Optimization of enzyme complexes for efficient hydrolysis of corn stover to produce glucose.
abstract: Hydrolysis of cellulose to glucose is the critical step for transferring the lignocellulose to the industrial chemicals. For improving the conversion rate of cellulose of corn stover to glucose, the cocktail of celllulase with other auxiliary enzymes and chemicals was studied in this work. Single factor tests and Response Surface Methodology (RSM) were applied to optimize the enzyme mixture, targeting maximum glucose release from corn stover. The increasing rate of glucan-to-glucose conversion got the higher levels while the cellulase was added 1.7μl tween-80/g cellulose, 300μg β-glucosidase/g cellulose, 400μg pectinase/g cellulose and 0.75mg/ml sodium thiosulphate separately in single factor tests. To improve the glucan conversion, the β-glucosidase, pectinase and sodium thiosulphate were selected for next step optimization with RSM. It is showed that the maximum increasing yield was 45.8% at 377μg/g cellulose Novozyme 188, 171μg/g cellulose pectinase and 1mg/ml sodium thiosulphate.
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Jiatong
authors.initials: J
authors.lastname: Meng
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Qiyue
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Zaixiao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Jiajing
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Qingfan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Life Science, Jilin University, Changchun, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ren
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D009097:Multienzyme Complexes; D011096:Polygalacturonase / Q000378:metabolism*; D011136:Polysorbates / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013885:Thiosulfates / Q000737:chemistry; D003313:Zea mays / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D011136:Polysorbates; D013501:Surface-Active Agents; D013885:Thiosulfates; C036909:lignocellulose; D008031:Lignin; C427304:Novozym 188; D011096:Polygalacturonase; D001617:beta-Glucosidase; D002480:Cellulase; C017717:sodium thiosulfate; D005947:Glucose