title: Preliminary study of genotoxicity evaluation of orthodontic miniscrews on mucosa oral cells by the alkaline comet assay.
abstract: Miniscrew implants are widely used nowadays in orthodontic treatments due to their good results in clinical practice. However, data regarding the biocompatibility of commercially available orthodontic miniscrews and temporary devices are very scarce, and their role as genotoxicity inducers has been not previously evaluated with the alkaline comet assay. The aim of this study was to investigate the DNA damage in buccal cells of patients subjected to orthodontic treatments. The alkaline comet assay has been applied in oral mucosa cells from patients treated with conventional orthodontic treatment in comparison to patients treated additionally with miniscrews, non-treated volunteers (control) and smoking volunteers (positive control). The application of orthodontic appliances and miniscrews induced significant and similar (2-fold) increases of %DNA in tail in comparison to control group. Females experienced a significant increase in %DNA in all the treatments in comparison to the control group, whereas males showed significant damage only with the combined orthodontic and miniscrew treatment. In conclusion, conventional orthodontic appliances induced genotoxicity, and the incorporation of miniscrews assayed did not imply any additional increase of DNA damage.
authors.affiliation: a Stomatology Department , School of Dentistry, University of Sevilla , Sevilla , Spain .
authors.forename: Ana
authors.initials: A
authors.lastname: Martín-Cameán
authors.affiliation: b Area of Toxicology, Faculty of Pharmacy, University of Sevilla , Sevilla , Spain .
authors.forename: María
authors.initials: M
authors.lastname: Puerto
authors.affiliation: b Area of Toxicology, Faculty of Pharmacy, University of Sevilla , Sevilla , Spain .
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Jos
authors.affiliation: c Department of Pharmacology and Toxicology, Faculty of Pharmacy , University of Navarra , Navarra , Spain , and.
authors.forename: Amaya
authors.initials: A
authors.lastname: Azqueta
authors.affiliation: d Stomatology Department , School of Dentistry, University Complutense of Madrid , Madrid , Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Iglesias-Linares
authors.affiliation: a Stomatology Department , School of Dentistry, University of Sevilla , Sevilla , Spain .
authors.forename: Enrique
authors.initials: E
authors.lastname: Solano
authors.affiliation: b Area of Toxicology, Faculty of Pharmacy, University of Sevilla , Sevilla , Spain .
authors.forename: Ana M
authors.initials: AM
authors.lastname: Cameán
mesh_terms: D000293:Adolescent; D000328:Adult; D001863:Bone Screws / Q000009:adverse effects*; D002648:Child; D020552:Comet Assay; D004249:DNA Damage; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008670:Metals / Q000009:adverse effects*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000187:drug effects* / Q000473:pathology; D052280:Orthodontic Anchorage Procedures / Q000009:adverse effects* / Q000295:instrumentation*; D018570:Risk Assessment; D055815:Young Adult
chemical_list: D008670:Metals
keywords: Buccal cells; comet assay; genotoxicity; miniscrew