title: Cellular and molecular markers in monitoring the fate of lymphoid cell culture from Penaeus monodon Fabricius (1798).
abstract: Lymphoid cell culture from penaeid shrimps has gained much acceptance as an in vitro platform to facilitate research on the development of prophylaxis, and therapeutic strategies against viruses and for cell line development. However, lymphoid cells can be used as platform for in vitro research, only if they are in metabolically and mitotically active state in vitro with unaltered cell surface receptors. Through this study, we addressed the response of lymphoid cells to a new microenvironment at cellular and molecular levels; including the study of mitotic events, DNA synthesis, expression profile of cell cycle genes, cytoskeleton organization, metabolic activity and viral susceptibility. The S-phase entry and synthesis of new DNA was recorded by immunoflourescent technique. Cdc2, CycA, CycB, EF-1α and BUB3 genes involved in cell cycle were studied in both the cells and tissue, of which EF-1α showed an elevated expression in cells in vitro (∼ 19.7%). Cytoskeleton network of the cell was examined by studying the organization of actin filaments. As the markers for metabolic status, mitochondrial dehydrogenase, protein synthesis and glucose assimilation by the cells were also assessed. Viral susceptibility of the cell was determined using WSSV to confirm the preservation of cellular receptors. This study envisages to strengthen the shrimp cell line research and to bring forth lymphoid cell culture system as a 'model' in vitro system for shrimp and crustaceans altogether.
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India; Department of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India. Electronic address: jayeshp24@gmail.com.
authors.forename: Jayesh
authors.initials: J
authors.lastname: Puthumana
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India.
authors.forename: Seena
authors.initials: S
authors.lastname: Jose
authors.affiliation: Department of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India.
authors.forename: Rosamma
authors.initials: R
authors.lastname: Philip
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682016, India. Electronic address: isbsingh@gmail.com.
authors.forename: I S Bright
authors.initials: IS
authors.lastname: Singh
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D008214:Lymphocytes / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D015415:Biomarkers
keywords: Cellular and molecular markers; Cytoskeleton network; Lymphoid cell culture; Penaeus monodon; Shrimp cell line