title: Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction.
abstract: Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.
authors.affiliation: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), QLD 4072, Australia. y.wang27@uq.edu.au m.trau@uq.edu.au.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.forename: Eugene J H
authors.initials: EJ
authors.lastname: Wee
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D018899:CpG Islands; D004247:DNA / Q000097:blood* / Q000378:metabolism; D019175:DNA Methylation; D006046:Gold / Q000737:chemistry; D022521:Ligase Chain Reaction; D053768:Metal Nanoparticles / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D006046:Gold; D004247:DNA