title: All-Aqueous Electrosprayed Emulsion for Templated Fabrication of Cytocompatible Microcapsules.
abstract: Encapsulation of biomolecules and cells in hydrogel capsules via emulsion templating frequently induces an irreversible loss of bioactivity, because of the use of nonaqueous solvents. Here, we introduce an all-aqueous electrospray (AAE) approach to generate aqueous two-phase emulsion droplets, and we use them as templates to fabricate microcapsules with preserved cell viability. The approach allows formation of monodisperse microparticles with tunable sizes, variable compositions, and interior architectures in a mild gelation process. This technique potentially benefits a variety of new biomedical applications, such as delivery of bioactive proteins, transplantation of living cells, and assembly of cell-mimicking structures.
authors.affiliation: ‡HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Guangdong 518000, China.|†Department of Mechanical Engineering, University of Hong Kong, Hong Kong.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: †Department of Mechanical Engineering, University of Hong Kong, Hong Kong.|§Department of Ophthalmology, University of Hong Kong, Hong Kong.
authors.forename: Yau Kei
authors.initials: YK
authors.lastname: Chan
authors.affiliation: ‡HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Guangdong 518000, China.|†Department of Mechanical Engineering, University of Hong Kong, Hong Kong.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Ma
authors.affiliation: ‡HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Guangdong 518000, China.|†Department of Mechanical Engineering, University of Hong Kong, Hong Kong.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Liu
authors.affiliation: ‡HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Guangdong 518000, China.|†Department of Mechanical Engineering, University of Hong Kong, Hong Kong.
authors.forename: Ho Cheung
authors.initials: HC
authors.lastname: Shum
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry*; D002214:Capsules / Q000138:chemical synthesis* / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D055664:Electrochemical Techniques / Q000379:methods*; D004655:Emulsions / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002214:Capsules; D004655:Emulsions; D020100:Hydrogels; D011092:Polyethylene Glycols
keywords: all-aqueous droplets; biomolecular assembly; cell encapsulation; core−shell microcapsules; electrospray; w/w/w double emulsion