title: Antibacterial and anti-biofilm activities of thiazolidione derivatives against clinical staphylococcus strains.
abstract: Both Staphylococcus aureus and Staphylococcus epidermidis can form biofilms on natural surfaces or abiotic surfaces, such as medical implants, resulting in biofilm-associated diseases that are refractory to antibiotic treatment. We previously reported a promising antibacterial compound (Compound 2) and its derivatives with bactericidal and anti-biofilm activities against both S. epidermidis and S. aureus. We have further evaluated the antibacterial activities of four Compound 2 derivatives (H2-38, H2-39, H2-74 and H2-81) against 163 clinical strains of S. epidermidis and S. aureus, including methicillin-susceptible and methicillin-resistant strains, as well as biofilm-forming and non-biofilm-forming strains. The four derivatives inhibited the planktonic growth of all of the clinical staphylococcal isolates, including methicillin-resistant S. aureus and methicillin-resistant S. epidermidis and displayed bactericidal activities against both immature (6 h) and mature (24 h) biofilms formed by the strong biofilm-forming strains. The derivatives, which all target YycG, will help us to develop new antimicrobial agents against multidrug-resistant staphylococci infections and biofilm-associated diseases.
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University , Shanghai 200032, China.
authors.forename: Huayong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Affiliated Gulou Hospital, Medical College of Nanjing University , Nanjing 210008, Jiangsu, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology , Nanjing 210009, Jiangsu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University , Shanghai 200032, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Gong
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University , Shanghai 200032, China.
authors.forename: Youcong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University , Shanghai 200032, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Han
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University , Shanghai 200032, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Cellular and Molecular Microbiology Division, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen , Tübingen 72076, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Peschel
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology , Nanjing 210009, Jiangsu, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Han
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University , Shanghai 200032, China.
authors.forename: Di
authors.initials: D
authors.lastname: Qu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002681:China; D006784:Hospitals, Teaching; D006801:Humans; D016106:Methicillin Resistance / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D012189:Retrospective Studies; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000502:physiology; D062606:Tertiary Care Centers; D013844:Thiazoles / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013844:Thiazoles
keywords: MIC50; Staphylococcus epidermidis; anti-biofilm activity; antibacterial; methicillin-resistant Staphylococcus aureus; minimal inhibitory concentration