title: Involvement of SF-1 in neurogenesis and neuronal migration in the developing neocortex.
abstract: The nuclear receptor steroidogenic factor-1 (SF-1) plays essential roles in the development and function of the endocrine and reproductive systems. During embryogenesis, SF-1 is expressed in the ventromedial hypothalamic nucleus (VMH) and regulates the migration and terminal differentiation of the VMH neurons. Additionally, in situ hybridization data indicated SF-1 expression in the dorsal telencephalon at embryonic day (E) 13.5. In this study, we investigated the neocortical development in SF-1 knockout (KO) mouse embryos. The number of neurons was increased in the intermediate/subventricular zones and decreased in the cortical plate in the SF-1 KO embryos. SF-1 KO embryos produced more neural stem/progenitor cells, especially apical progenitor cells, and showed abnormal radial glial fiber morphology. The increase in neural stem/progenitor cells was caused by an increased S-phase fraction in the proliferative cells and the inhibition of cell cycle exit in these cells. The mRNA expression of the estrogen receptor ESRα was up-regulated and that of the estrogen synthetase Cyp19a1 was down-regulated in the dorsal telencephalon of SF-1 KO embryos. We showed that SF-1 is expressed in the dorsal telencephalon at E15.5 and E18.5, but not in adult animals. Our data demonstrated that SF-1 is involved in cell cycle regulation, neurogenesis, and neuronal migration via controlling the estrogen signaling for proper neocortical development.
authors.affiliation: Department of Anatomy, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Munekazu
authors.initials: M
authors.lastname: Komada
authors.affiliation: Department of Anatomy, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors.forename: Mifumi
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Anatomy, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. Electronic address: yayoi@dpc.agu.ac.jp.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology / Q000196:embryology; D051379:Mice; D018345:Mice, Knockout; D019579:Neocortex / Q000166:cytology* / Q000196:embryology; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D054339:Steroidogenic Factor 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D054339:Steroidogenic Factor 1; D001141:Aromatase
keywords: Cell cycle; Dorsal telencephalon; Estrogen signaling; Neurogenesis; Proliferation; Steroidogenic factor-1