title: Characteristic miR-24 Expression in Gastric Cancers among Atomic Bomb Survivors.
abstract: OBJECTIVE
  To elucidate the mechanism of radiation-induced cancers, we analyzed the expression profiles of microRNAs extracted from formalin-fixed paraffin-embedded (FFPE) gastric cancer (GC) tissue samples from atomic bomb survivors.
  
  
  METHODS
  The expression levels of miR-21, miR-24, miR-34a, miR-106a, miR-143, and miR-145 were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  The expression of microRNAs was measured by qRT-PCR in a Hiroshima University Hospital cohort comprising 32 patients in the high-dose-exposed group and 18 patients in the low-dose-exposed group who developed GC after the bombing. The GC cases showing high expression of miR-24, miR-143, and miR-145 were more frequently found in the high-dose-exposed group than in the low-dose-exposed group. We next performed qRT-PCR of miR-24, miR-143, and miR-145 in a cohort from the Hiroshima Red Cross Hospital and Atomic-Bomb Survivors Hospital comprising 122 patients in the high-dose-exposed group and 48 patients in the low-dose-exposed group who developed GC after the bombing. High expressions of miR-24 and miR-143 were more frequently found in the high-dose-exposed group than in the low-dose-exposed group. Multivariate analysis demonstrated that only high expression of miR-24 was an independent predictor for the exposure status.
  
  
  CONCLUSIONS
  These results suggest that the measurement of miR-24 expression from FFPE samples is useful to identify radiation-associated GC.
authors.affiliation: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Naito
authors.forename: Naohide
authors.initials: N
authors.lastname: Oue
authors.forename: Trang T B
authors.initials: TT
authors.lastname: Pham
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Megumu
authors.initials: M
authors.lastname: Fujihara
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Ishida
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Mukai
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sentani
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Eisuke
authors.initials: E
authors.lastname: Hida
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Wataru
authors.initials: W
authors.lastname: Yasui
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D054043:Nuclear Weapons; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics*; D017741:Survivors
chemical_list: D015415:Biomarkers; C543845:MIRN24 microRNA, human; D035683:MicroRNAs