title: Biotinylated magnetic nanoparticles for pretargeting: synthesis and characterization study.
abstract: In this paper, we have proposed a simple method to covalently conjugate biotin to magnetic nanoparticles, which can be targeted to the tumour sites by using pretargeting approach with avidin or streptavidin. Magnetic nanoparticles of manganese ferrite were synthesized by alkaline coprecipitation of ferric chloride hexahydrate, ferrous sulphate heptahydrate and manganese sulphate monohydrate using ammonium hydroxide. The synthesized magnetic nanoparticles were then successfully surface modified by using 3-aminopropyl trimethoxysilane, and the amount of aminopropylsilane bound to the surface of magnetic nanoparticles was quantified by measuring the absorbance of a purple-coloured complex (Ruhemann's purple) formed between amine group and ninhydrin at 576 nm. The aminated magnetic nanoparticles were then conjugated to biotin by reacting them withN-hydroxysuccinimide-biotin in dimethylsulphoxide. The successful conjugation of biotin to magnetic nanoparticles was confirmed by Fourier transform infrared spectroscopy. The size, phase and magnetic nature of the synthesized nanoparticles were analysed by using various techniques like transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy and vibrating sample magnetometry.
authors.affiliation: Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Brig.S.K.Mazumdar Marg, Delhi, 110054 India ; Department of Chemistry, Panjab University, Chandigarh, 160014 India.
authors.forename: Ram Prakash
authors.initials: RP
authors.lastname: Chauhan
authors.affiliation: Department of Chemistry, Panjab University, Chandigarh, 160014 India.
authors.forename: Gurjaspreet
authors.initials: G
authors.lastname: Singh
authors.affiliation: Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Brig.S.K.Mazumdar Marg, Delhi, 110054 India.
authors.forename: Sweta
authors.initials: S
authors.lastname: Singh
authors.affiliation: Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Brig.S.K.Mazumdar Marg, Delhi, 110054 India.
authors.forename: Narmada
authors.initials: N
authors.lastname: Bag
authors.affiliation: Defence Laboratory, Ratanada Palace Road, Jodhpur, 342011 India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Patra
authors.affiliation: Defence Laboratory, Ratanada Palace Road, Jodhpur, 342011 India.
authors.forename: S R
authors.initials: SR
authors.lastname: Vadera
authors.affiliation: Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Brig.S.K.Mazumdar Marg, Delhi, 110054 India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Mishra
authors.affiliation: Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Brig.S.K.Mazumdar Marg, Delhi, 110054 India.
authors.forename: Rashi
authors.initials: R
authors.lastname: Mathur
keywords: 3-Aminopropyltrimethoxysilane (APS); Biotinylation; EDX; Pretargeted magnetic nanoparticles; Ruhemann’s purple; Superparamagnetic behaviour