title: Preparation, in vitro mineralization and osteoblast cell response of electrospun 13-93 bioactive glass nanofibers.
abstract: In this study, silicate based 13-93 bioactive glass fibers were prepared through sol-gel processing and electrospinning technique. A precursor solution containing poly (vinyl alcohol) and bioactive glass sol was used to produce fibers. The mixture was electrospun at a voltage of 20 kV by maintaining tip to a collector distance of 10 cm. The amorphous glass fibers with an average diameter of 464±95 nm were successfully obtained after calcination at 625 °C. Hydroxyapatite formation on calcined 13-93 fibers was investigated in simulated body fluid (SBF) using two different fiber concentrations (0.5 and 1 mg/ml) at 37 °C. When immersed in SBF, conversion to a calcium phosphate material showed a strong dependence on the fiber concentration. At 1mg/ml, the surface of the fibers converted to the hydroxyapatite-like material in SBF only after 30 days. At lower solid concentrations (0.5 mg/ml), an amorphous calcium phosphate layer formation was observed followed by the conversion to hydroxyapatite phase after 7 days of immersion. The XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) assay was conducted to evaluate the osteoblast cell response to the bioactive glass fibers.
authors.affiliation: Celal Bayar University, Department of Materials Engineering, Manisa, Turkey. Electronic address: aylin.deliormanli@cbu.edu.tr.
authors.forename: Aylin M
authors.initials: AM
authors.lastname: Deliormanlı
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D055664:Electrochemical Techniques; D005898:Glass / Q000737:chemistry*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects; D010316:Particle Size; D013499:Surface Properties
chemical_list: C553965:13-93 bioactive glass; D001672:Biocompatible Materials
keywords: Bioactive glass; Electrospinning; Fibers; Tissue engineering applications