title: Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3.
abstract: Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structural proteins are unclear. In this study, we used codon recoding to express the recombinant proteins VP1, VP2, and VP3 in Escherichia coli. SDS-PAGE analysis and Western blotting revealed that the target genes were expressed at high levels. Mice were then immunized with the purified, recombinant proteins, and antibody levels and lymphocyte proliferation were analyzed by ELISA and the MTT assay, respectively. The results show that the recombinant proteins induced high antibody levels and promoted lymphocyte proliferation. Polyclonal antibodies directed against these proteins will aid future studies of the molecular pathogenesis of CSBV.
authors.affiliation: Animal Husbandry and Veterinary Institute, Liaoning Medical University, Jinzhou, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Fei
authors.affiliation: Animal Husbandry and Veterinary Institute, Liaoning Medical University, Jinzhou, China.
authors.forename: Haochun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Honeybee Research Institute, the Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Diao
authors.affiliation: Animal Husbandry and Veterinary Institute, Liaoning Medical University, Jinzhou, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Liaoning Water Conservancy Vocational College, Shenyang, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Animal Husbandry and Veterinary Institute, Liaoning Medical University, Jinzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Animal Husbandry and Veterinary Institute, Liaoning Medical University, Jinzhou, China.
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Animal Husbandry and Veterinary Institute, Liaoning Medical University, Jinzhou, China.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Ma
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology*; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D010957:Plasmids / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D003062:Codon; D007074:Immunoglobulin G; D011994:Recombinant Proteins