title: Needle-free and adjuvant-free epicutaneous boosting of pertussis immunity: Preclinical proof of concept.
abstract: The limited durability of pertussis vaccine-induced protection requires novel approaches to reactivate immunity and limit pertussis resurgence in older children and adults. We propose that periodic boosters could be delivered using a novel epicutaneous delivery system (Viaskin) to deliver optimized pertussis antigens such as genetically-detoxified pertussis toxin (rPT). To best mimic the human situation in which vaccine-induced memory cells persist, whereas antibodies wane, we developed a novel adoptive transfer murine model of pertussis immunity. This allowed demonstrating that a single application of Viaskin delivering rPT and/or pertactin and filamentous hemagglutinin effectively reactivates vaccine-induced pertussis immunity and protects against Bordetella pertussis challenge. Recalling pertussis immunity without needles nor adjuvant may considerably facilitate the acceptance and application of periodic boosters.
authors.affiliation: World Health Organization Collaborating Center for Vaccine Immunology, Departments of Pathology-Immunology, University of Geneva, 1211 Geneva, Switzerland. Electronic address: beatris.mastelic@unige.ch.
authors.forename: Beatris Mastelic
authors.initials: BM
authors.lastname: Gavillet
authors.affiliation: DBV Technologies, Green Square, 80/84 rue des Meuniers, 92220 Bagneux, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Mondoulet
authors.affiliation: DBV Technologies, Green Square, 80/84 rue des Meuniers, 92220 Bagneux, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Dhelft
authors.affiliation: World Health Organization Collaborating Center for Vaccine Immunology, Departments of Pathology-Immunology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Christiane Sigrid
authors.initials: CS
authors.lastname: Eberhardt
authors.affiliation: World Health Organization Collaborating Center for Vaccine Immunology, Departments of Pathology-Immunology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Floriane
authors.initials: F
authors.lastname: Auderset
authors.affiliation: BioNet-Asia Co., Ltd., 19 Udomsuk 37, Sukhumvit 103, Bangjak, Prakanong, Bangkok 10260, Thailand.
authors.forename: Hong Thai
authors.initials: HT
authors.lastname: Pham
authors.affiliation: BioNet-Asia Co., Ltd., 19 Udomsuk 37, Sukhumvit 103, Bangjak, Prakanong, Bangkok 10260, Thailand.
authors.forename: Jean
authors.initials: J
authors.lastname: Petre
authors.affiliation: World Health Organization Collaborating Center for Vaccine Immunology, Departments of Pathology-Immunology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Paul-Henri
authors.initials: PH
authors.lastname: Lambert
authors.affiliation: DBV Technologies, Green Square, 80/84 rue des Meuniers, 92220 Bagneux, France.
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Benhamou
authors.affiliation: World Health Organization Collaborating Center for Vaccine Immunology, Departments of Pathology-Immunology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Claire-Anne
authors.initials: CA
authors.lastname: Siegrist
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D007117:Immunization, Secondary / Q000379:methods*; D008807:Mice, Inbred BALB C; D010567:Pertussis Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D014917:Whooping Cough / Q000517:prevention & control*
chemical_list: D000942:Antigens, Bacterial; D010567:Pertussis Vaccine
keywords: Adjuvant-free; Epicutaneous; Needle-free; Pertussis; Vaccine