title: In situ analysis of epigenetic modifications in the chromatin of Brachypodium distachyon embryos.
abstract: Epigenetic modifications of the chromatin structure are crucial for many biological processes and act on genes during the development and germination of seeds. The spatial distribution of 3 epigenetic markers, i.e. H4K5ac, H3K4me2 and H3K4me1 was investigated in 'matured,' 'dry,' 'imbibed" and 'germinating' embryos of a model grass, Brachypodium. Our results indicate that the patterns of epigenetic modification differ in the various types of tissues of embryos that were analyzed. Such a tissue-specific manner of these modifications may be linked to the switch of the gene expression profiles in various organs of the developing embryo.
authors.affiliation: a Department of Plant Anatomy and Cytology; Faculty of Biology and Environmental Protection; University of Silesia in Katowice , Katowice , Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Wolny
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Braszewska-Zalewska
authors.forename: Daria
authors.initials: D
authors.lastname: Kroczek
authors.forename: Robert
authors.initials: R
authors.lastname: Hasterok
mesh_terms: D058431:Brachypodium / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D044127:Epigenesis, Genetic; D012639:Seeds / Q000378:metabolism*
chemical_list: D002843:Chromatin
keywords: B. distachyon; Brachypodium, Brachypodium distachyon; DAPI, 4′, 6 diamidino-2-phenylindole; H3K4me1; H3K4me1, histone H3 methylation at lysine 4; H3K4me2; H3K4me2, histone H4 dimethylation at lysine 4; H4K5ac; H4K5ac, histone H4 acetylation at lysine 5; Poaceae; RAM, root apical meristem; SAM, shoot apical meristem; chromatin; embryo; epigenetic modifications; immunostaining techniques; organ; tissue