title: Relationship between the Increased Haemostatic Properties of Blood Platelets and Oxidative Stress Level in Multiple Sclerosis Patients with the Secondary Progressive Stage.
abstract: Multiple sclerosis (MS) is the autoimmune disease of the central nervous system with complex pathogenesis, different clinical courses and recurrent neurological relapses and/or progression. Despite various scientific papers that focused on early stage of MS, our study targets selective group of late stage secondary progressive MS patients. The presented work is concerned with the reactivity of blood platelets in primary hemostasis in SP MS patients. 50 SP MS patients and 50 healthy volunteers (never diagnosed with MS or other chronic diseases) were examined to evaluate the biological activity of blood platelets (adhesion, aggregation), especially their response to the most important physiological agonists (thrombin, ADP, and collagen) and the effect of oxidative stress on platelet activity. We found that the blood platelets from SP MS patients were significantly more sensitive to all used agonists in comparison with control group. Moreover, the platelet hemostatic function was advanced in patients suffering from SP MS and positively correlated with increased production of O2 (-∙) in these cells, as well as with Expanded Disability Status Scale. We postulate that the increased oxidative stress in blood platelets in SP MS may be primarily responsible for the altered haemostatic properties of blood platelets.
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Agnieszka
authors.identifier: 0000-0003-3634-3606
authors.initials: A
authors.lastname: Morel
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Bijak
authors.affiliation: Department of Physical Medicine, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland ; Neurorehabilitation Ward, III General Hospital in Lodz, Milionowa 14, 93-113 Lodz, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Miller
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Joanna
authors.identifier: 0000-0001-8895-7082
authors.initials: J
authors.lastname: Rywaniak
authors.affiliation: Department of Orthodontics, Medical University in Lodz, Pomorska 251, 92-213 Lodz, Poland.
authors.forename: Sergiusz
authors.initials: S
authors.lastname: Miller
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland ; Department of Toxicology, Faculty of Pharmacy with Division of Medical Analytics, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.
authors.forename: Joanna
authors.identifier: 0000-0002-1455-443X
authors.initials: J
authors.lastname: Saluk
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D016022:Case-Control Studies; D003094:Collagen / Q000494:pharmacology; D005260:Female; D005340:Fibrinogen / Q000494:pharmacology; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects*; D010973:Platelet Adhesiveness / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology
chemical_list: D006490:Hemostatics; D013481:Superoxides; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D003094:Collagen; D013917:Thrombin