title: miR-449b rs10061133 and miR-4293 rs12220909 polymorphisms are associated with decreased esophageal squamous cell carcinoma in a Chinese population.
abstract: Esophageal cancer is one of the most aggressive cancers in the world, 70% of which are from China and esophageal squamous cell carcinoma (ESCC) is the major histopathological form (>90%). The single nucleotide polymorphisms (SNPs) in mature sequence of microRNA (miRNA) (mmSNPs) could cause the alteration of microRNA expression and contribute to the susceptibility of cancers. To evaluate the association between mmSNPs and ESCC, a case-control study including 773 patients with ESCC and 882 gender- and age-matched controls was carried out to investigate the association of five mmSNPs (miR-449b rs10061133, miR-4293 rs12220909, miR-608 rs4919510, miR-627 rs2620381, and miR-646 rs6513497) with ESCC susceptibility. As a result, two SNPs, miR-449b rs10061133 and miR-4293 rs12220909, were associated with decreased ESCC risk. For miR-449b rs10061133 A>G, individuals carrying GG genotype had an odds ratio (OR) of 0.77 (95% confidence interval (95% CI) 0.62-0.97) compared with individuals with AA genotype. In the recessive model, the GG genotype also showed a protective effect on ESCC (OR = 0.78, 95% CI 0.63-0.97). For miR-4293 rs12220909 G>C, the heterozygous genotype GC was associated with a decreased ESCC risk (OR = 0.77, 95% CI 0.61-0.97) compared with GG genotype. The C allele conferred 23% decrease in ESCC risk compared with the G allele in the allelic model (95% CI 0.63-0.93). In the dominant model, the GC/CC genotypes decreased the risk of ESCC (adjusted OR = 0.77, 95% CI 0.61-0.96). This study provides the first evidence that miR-449b rs10061133 and miR-4293 rs12220909 are associated with ESCC risk in Chinese population.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.|Department of Medical Genetics, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lu
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Yabiao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Cancer Research Laboratory, Fudan University Shanghai Cancer Center, Shanghai, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Mengyun
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Kolluri
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.|Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Shen
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.|Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastric Cancer & Soft Tissue Sarcoma Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
authors.forename: Yanong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Research Laboratory, Fudan University Shanghai Cancer Center, Shanghai, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Qingyi
authors.initials: Q
authors.lastname: Wei
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China. jcwang@fudan.edu.cn.
authors.forename: Jiucun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu, China. mhwang@suda.edu.cn.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002681:China; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D014408:Biomarkers, Tumor; C000606151:MIRN4293 microRNA, human; C544530:MIRN449 microRNA, human; D035683:MicroRNAs
keywords: Esophageal squamous cell carcinoma; Susceptibility; miRNA; mmSNPs