title: Inhibiting Smooth Muscle Cell Proliferation via Immobilization of Heparin/Fibronectin Complexes on Titanium Surfaces.
abstract: The aim of this study was to investigate the inhibitory effect of heparin/fibronectin (Hep/Fn) complexes on neointimal hyperplasia following endovascular intervention. Hep/Fn complexes were immobilized onto titanium (Ti) surfaces, with subsequent X-ray photoelectron spectroscopy (XPS), Toluidine Blue O (TBO) and immunohistochemistry methods were used to characterize surface properties. Smooth muscle cell (SMC) cultures were used to evaluate the effect of Hep/Fn complexes on SMC proliferation. Results showed that Hep/Fn complexes successfully immobilized onto Ti surfaces and resulted in an inhibition of SMC proliferation. This study suggests that Hep/Fn surface-immobilized biomaterials develop as a new generation of biomaterials to prevent neointimal hyperplasia, particularly for use in cardiovascular implants.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; Key Laboratory for Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, Sichuan, China.
authors.forename: Gui Cai
authors.initials: GC
authors.lastname: Li
authors.affiliation: Department of Radiology, Linyi People's Hospital, Linyi 276000, Shandong, China.
authors.forename: Qi Fei
authors.initials: QF
authors.lastname: Xu
authors.affiliation: Key Laboratory for Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, Sichuan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
mesh_terms: D001672:Biocompatible Materials; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014469:Umbilical Arteries
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D055767:Immobilized Proteins; D006493:Heparin; D014025:Titanium