title: Characterization and comparative analysis of a simian foamy virus complete genome isolated from Brazilian capuchin monkeys.
abstract: Foamy viruses infect a wide range of placental mammals, including primates. However, despite of great diversity of New World primates, only three strains of neotropical simian foamy viruses (SFV) have been described. Only after 40 years since serological characterization, the complete sequence of an SFVcap strain infecting a family of six capuchin monkeys (Sapajus xanthosternos) was obtained. Co-culture of primate peripheral blood mononuclear cells with Cf2Th canine cells was established and monitored for the appearance of cytopathic effects, PCR amplification of integrated SFV proviral genome and viral reverse transcriptase activity. The novel SFVcap was fully sequenced through a next-generation sequencing protocol. Phylogenetic analysis of the complete genome grouped SFVcap and SFVmar, both infecting primate species of the Cebidae family with a genetic similarity of approximately 85%. Similar ORF sizes were observed among SFV from neotropical primates, and env and pol genes were the most conserved. Neotropical SFV presented the smallest LTRs among exogenous mammalians. The novel SFVcap strain provides a valuable research tool for the FV community.
authors.affiliation: Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A, sala A2-120, Cidade Universitária, 21949-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Lian L
authors.initials: LL
authors.lastname: Troncoso
authors.affiliation: Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A, sala A2-120, Cidade Universitária, 21949-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Cláudia P
authors.initials: CP
authors.lastname: Muniz
authors.affiliation: Programa de Oncovirologia, Instituto Nacional de Câncer, Rua André Cavalcanti, 37 - 4° andar, Bairro de Fátima, 20231-050 Rio de Janeiro, RJ, Brazil.
authors.forename: Juliana D
authors.initials: JD
authors.lastname: Siqueira
authors.affiliation: Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A, sala A2-120, Cidade Universitária, 21949-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Gislaine
authors.initials: G
authors.lastname: Curty
authors.affiliation: Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A, sala A2-120, Cidade Universitária, 21949-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Schrago
authors.affiliation: Fundação Jardim Zoológico da Cidade do Rio de Janeiro, Parque da Quinta Boa Vista, s/n - São Cristovão, 20940-040 Rio de Janeiro, RJ, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Augusto
authors.affiliation: Fundação Jardim Zoológico da Cidade do Rio de Janeiro, Parque da Quinta Boa Vista, s/n - São Cristovão, 20940-040 Rio de Janeiro, RJ, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Fedullo
authors.affiliation: Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A, sala A2-120, Cidade Universitária, 21949-570 Rio de Janeiro, RJ, Brazil; Programa de Oncovirologia, Instituto Nacional de Câncer, Rua André Cavalcanti, 37 - 4° andar, Bairro de Fátima, 20231-050 Rio de Janeiro, RJ, Brazil.
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Soares
authors.affiliation: Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A, sala A2-120, Cidade Universitária, 21949-570 Rio de Janeiro, RJ, Brazil. Electronic address: andre.santos@ufrj.br.
authors.forename: André F
authors.initials: AF
authors.lastname: Santos
mesh_terms: D000818:Animals; D001938:Brazil; D002428:Cebus / Q000821:virology; D004285:Dogs; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D010802:Phylogeny; D012192:Retroviridae Infections / Q000662:veterinary* / Q000821:virology; D053150:Simian foamy virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
keywords: Capuchin monkeys; Foamy virus; New World primates; Next-generation sequencing; SFVcap; Spumavirus