title: Eight-year follow-up study of three individuals accidentally exposed to (60)Co radiation: Chromosome aberration and micronucleus analysis.
abstract: We assessed dose levels and the persistence of chromosomal aberrations and micronuclei in three individuals in the 8 year following accidental (60)Co radiation exposure. Venous blood samples were collected and used for analyses: traditional chromosome aberration (CA) measurement, G-banding, and the cytokinesis-block micronucleus (CBMN) assay. For CA analysis, we scored dicentric chromosomes (dic) and rings (r) in peripheral blood lymphocytes. The radiation doses (Gy) suffered by the individuals were estimated as: 1.79-2.43 (A), 2.36-2.86 (B), 1.58-1.82 (C), based on CA analysis; and 1.8-2.34 (A), 2.52-2.98 (B), 1.53-1.77 (C), based on CBMN frequencies. G-banding analysis was used to record translocations (t), inversions (inv), and deletions (del). Following the accident, unstable CAs reduced gradually, but stable aberrations persisted. Unstable CAs and CBMN may be valuable biomarkers for dose estimation shortly after high-dose radiation accidents, while stable aberrations may be more useful for assessing long-term effects.
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, PR China; Qianfoshan Hospital of Shandong Province, PR China; Radiation Medical Institute, Shandong Academy of Medical Sciences, Jinan 250062, PR China. Electronic address: qqddfl1998@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Center of Disease Control and Prevention, Sichuan Province 610041, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, PR China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Du
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, PR China. Electronic address: liuqiang@irm-cams.ac.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, PR China. Electronic address: fanfeiyue10@126.com.
authors.forename: Feiyue
authors.initials: F
authors.lastname: Fan
mesh_terms: D054508:Acute Radiation Syndrome / Q000235:genetics* / Q000473:pathology*; D000328:Adult; D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes / Q000633:toxicity*; D020732:Cytogenetic Analysis / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests / Q000379:methods; D018788:Radioactive Hazard Release
chemical_list: D003037:Cobalt Radioisotopes
keywords: Cytogenetic analysis; Dose estimation; Ionizing radiation; Radiation accident