title: The Ankyrin Repeat Domain 49 (ANKRD49) Augments Autophagy of Serum-Starved GC-1 Cells through the NF-κB Pathway.
abstract: The ankyrin repeat domain 49 (ANKRD49) is an evolutionarily conserved protein highly expressed in testes. However, the function of ANKRD49 in spermatogenesis is unknown. In this study, we found that ANKRD49 resides primarily in nucleus of spermatogonia, spermatocytes and round spermatids. ANKRD49 overexpression augments starvation-induced autophagy in male germ GC-1 cells whereas shRNA knockdown of ANKRD49 attenuates the autophagy. Inhibition of NF-κB pathway by its inhibitors or p65 siRNA prevents the ANKRD49-dependent autophagy augmentation, demonstrating that ANKRD49 enhances autophagy via NF-κB pathway. Our findings suggest that ANKRD49 plays an important role in spermatogenesis via promotion of autophagy-dependent survival.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Sha-sha
authors.initials: SS
authors.lastname: Fan
authors.affiliation: Department of Respiratory, the First Affiliated Hospital, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Pang
authors.affiliation: Class 041002, Department of Anestesioloy, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Yi-heng
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Center of Laboratory Animal, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, 5 Dong Dan San Tiao, Beijing, 100005, China.
authors.forename: Jun-bo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, PR China.
authors.forename: Guo-ping
authors.initials: GP
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D001343:Autophagy / Q000187:drug effects; D002460:Cell Line; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D008297:Male; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011418:Prophase / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D016895:Culture Media, Serum-Free; D009124:Muscle Proteins; D016328:NF-kappa B; C000607223:ankyrin repeat domain 49 protein, mouse