title: The adpA-like regulatory gene from Actinoplanes teichomyceticus: in silico analysis and heterologous expression.
abstract: Analysis of the draft sequence of the genome of teicoplanin producer Actinoplanes teichomyceticus (NRRL-B16726) led to identification of several genes encoding AraC-family regulators that resemble AdpA, master regulator of transcription in Streptomyces. We elucidated possible regulatory functions of one of the identified genes, adpA19(at), most similar to archetypal adpA from model Streptomyces species, in a series of expression experiments. Introduction of adpA19 at under control of its own promoter on moderate copy number vector pKC1139 into NRRL-B16726 had no influence on antibiotic production and sporulation. Introduction of adpA19 at into Streptomyces coelicolor M145 and several S. ghanaensis strains had major influence on antibiotic production by these bacteria. Finally, adpA19 at expression in a set of soil actinomycete isolates led to induction of synthesis of antibiotic compounds. Our data point to pleiotropic regulatory role of adpA19(at), warranting its use as a tool to manipulate secondary metabolome of actinomycetes.
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st., Rm. 104, Lviv, 79005, Ukraine.
authors.forename: Bohdan
authors.initials: B
authors.lastname: Ostash
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Yushchuk
authors.forename: Stepan
authors.initials: S
authors.lastname: Tistechok
authors.forename: Halyna
authors.initials: H
authors.lastname: Mutenko
authors.forename: Lilia
authors.initials: L
authors.lastname: Horbal
authors.forename: Andriy
authors.initials: A
authors.lastname: Muryn
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Dacyuk
authors.forename: Jorn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
authors.forename: Victor
authors.initials: V
authors.lastname: Fedorenko
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003198:Computer Simulation; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008848:Micromonosporaceae / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins