title: The actions of neurotensin in rat bladder detrusor contractility.
abstract: This study assessed the expression, distribution and function of neurotensin (NTs) and two main neurotensin receptors (NTSR), NTSR1 and NTSR2 in normal rat urinary bladders. NTs is primarily located in the suburothelium and the interstitium of smooth muscle bundles. The NTSR1 and NTSR2 receptor subtypes are found to co-localize with smooth muscle cells (SMCs). NTs not only can directly act on bladder SMCs to induce intracellular calcium mobilization by activating the phospholipase C/inositol triphosphate (PLC/IP3) pathway, promoting extracellular calcium influx through a non-selective cation channels, but may be also involved in the modulation of the cholinergic system. Nowadays, the selective antimuscarinic drugs (solifenacin) and the selective beta 3-adrenergic agonist (mirabegron) are used as the first-line pharmacotherapy for overactive bladder (OAB), but without satisfactory treatment benefits in some patients. This study provided evidence suggesting that bladder NTs may play an important role in the regulation of micturition. Further research is needed to investigate the effects of NTs on bladder contractility and the underlying mechanism, which might reveal that the administration of NTSR antagonists can potentially relieve the symptoms of OAB by coordination with antimuscarinic pharmacotherapy.
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Xingyou
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Longkun
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D059329:Cholinergic Neurons / Q000378:metabolism; D005260:Female; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009496:Neurotensin / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018028:Receptors, Neurotensin / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D001743:Urinary Bladder / Q000502:physiology*; D053201:Urinary Bladder, Overactive / Q000503:physiopathology*
chemical_list: D018727:Muscarinic Antagonists; C099727:Ntsr2 protein, rat; D018028:Receptors, Neurotensin; C114036:neurotensin type 1 receptor; D009496:Neurotensin; D002118:Calcium