title: Combination of Autoantibody Signature with PSA Level Enables a Highly Accurate Blood-Based Differentiation of Prostate Cancer Patients from Patients with Benign Prostatic Hyperplasia.
abstract: Although an increased level of the prostate-specific antigen can be an indication for prostate cancer, other reasons often lead to a high rate of false positive results. Therefore, an additional serological screening of autoantibodies in patients' sera could improve the detection of prostate cancer. We performed protein macroarray screening with sera from 49 prostate cancer patients, 70 patients with benign prostatic hyperplasia and 28 healthy controls and compared the autoimmune response in those groups. We were able to distinguish prostate cancer patients from normal controls with an accuracy of 83.2%, patients with benign prostatic hyperplasia from normal controls with an accuracy of 86.0% and prostate cancer patients from patients with benign prostatic hyperplasia with an accuracy of 70.3%. Combining seroreactivity pattern with a PSA level of higher than 4.0 ng/ml this classification could be improved to an accuracy of 84.1%. For selected proteins we were able to confirm the differential expression by using luminex on 84 samples. We provide a minimally invasive serological method to reduce false positive results in detection of prostate cancer and according to PSA screening to distinguish men with prostate cancer from men with benign prostatic hyperplasia.
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Leidinger
authors.affiliation: Chair for Clinical Bioinformatics, Saarland University, Building E.2.1, 66123 Saarbrücken, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Keller
authors.affiliation: Austrian Institute of Technology GmbH, Health & Environment Department, 1220 Wien, Austria.
authors.forename: Lisa
authors.initials: L
authors.lastname: Milchram
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Harz
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hart
authors.affiliation: Medical Practice of Urology, 66877 Ramstein-Miesenbach, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Werth
authors.affiliation: Center for Bioinformatics, Saarland University, Building E.1.1, 66123 Saarbrücken, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Lenhof
authors.affiliation: Austrian Institute of Technology GmbH, Health & Environment Department, 1220 Wien, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Weinhäusel
authors.affiliation: University Clinic of Urology, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Keck
authors.affiliation: University Clinic of Urology, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wullich
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Ludwig
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D002999:Clone Cells; D003937:Diagnosis, Differential; D006801:Humans; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000097:blood*; D011470:Prostatic Hyperplasia / Q000097:blood* / Q000175:diagnosis*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D017430:Prostate-Specific Antigen