title: Evaluation of anti-hyperglycemic effect of Actinidia kolomikta (Maxim. etRur.) Maxim. root extract.
abstract: This study aimed to evaluate the anti-hyperglycemic effect of ethanol extract from Actinidia kolomikta (Maxim. etRur.) Maxim. root (AKE).An in vitro evaluation was performed by using rat intestinal α-glucosidase (maltase and sucrase), the key enzymes linked with type 2 diabetes. And an in vivo evaluation was also performed by loading maltose, sucrose, glucose to normal rats. As a result, AKE showed concentration-dependent inhibition effects on rat intestinal maltase and rat intestinal sucrase with IC(50) values of 1.83 and 1.03mg/mL, respectively. In normal rats, after loaded with maltose, sucrose and glucose, administration of AKE significantly reduced postprandial hyperglycemia, which is similar to acarbose used as an anti-diabetic drug. High contents of total phenolics (80.49 ± 0.05mg GAE/g extract) and total flavonoids (430.69 ± 0.91mg RE/g extract) were detected in AKE. In conclusion, AKE possessed anti-hyperglycemic effects and the possible mechanisms were associated with its inhibition on α-glucosidase and the improvement on insulin release and/or insulin sensitivity as well. The anti-hyperglycemic activity possessed by AKE maybe attributable to its high contents of phenolic and flavonoid compounds.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Xuansheng
authors.initials: X
authors.lastname: Hu
authors.affiliation: Taisei Kogyo Co., Ltd, Minamiotsuka, Toshima-ku, Tokyo, Japan.
authors.forename: Delin
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Natural Product Industry of Tsukuba Co., Ltd, East Hiratuka, Tsukuba, Japan.
authors.forename: Linbo
authors.initials: L
authors.lastname: Wang
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Li
authors.affiliation: Natural Product Industry of Tsukuba Co., Ltd, East Hiratuka, Tsukuba, Japan.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Kejuan
authors.initials: K
authors.lastname: Li
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D029042:Actinidia / Q000737:chemistry; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000494:pharmacology; D005951:Glucose Tolerance Test; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010636:Phenols / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000737:chemistry; D013393:Sucrase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013997:Time Factors; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005419:Flavonoids; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; D000520:alpha-Glucosidases; D013393:Sucrase