title: Mechanisms linking metabolism of Helicobacter pylori to (18)O and (13)C-isotopes of human breath CO2.
abstract: The gastric pathogen Helicobacter pylori utilize glucose during metabolism, but the underlying mechanisms linking to oxygen-18 ((18)O) and carbon-13 ((13)C)-isotopic fractionations of breath CO2 during glucose metabolism are poorly understood. Using the excretion dynamics of (18)O/(16)O and (13)C/(12)C-isotope ratios of breath CO2, we found that individuals with Helicobacter pylori infections exhibited significantly higher isotopic enrichments of (18)O in breath CO2 during the 2h-glucose metabolism regardless of the isotopic nature of the substrate, while no significant enrichments of (18)O in breath CO2 were manifested in individuals without the infections. In contrast, the (13)C-isotopic enrichments of breath CO2 were significantly higher in individuals with Helicobacter pylori compared to individuals without infections in response to (13)C-enriched glucose uptake, whereas a distinguishable change of breath (13)C/(12)C-isotope ratios was also evident when Helicobacter pylori utilize natural glucose. Moreover, monitoring the (18)O and (13)C-isotopic exchange in breath CO2 successfully diagnosed the eradications of Helicobacter pylori infections following a standard therapy. Our findings suggest that breath (12)C(18)O(16)O and (13)C(16)O(16)O can be used as potential molecular biomarkers to distinctively track the pathogenesis of Helicobacter pylori and also for eradication purposes and thus may open new perspectives into the pathogen's physiology along with isotope-specific non-invasive diagnosis of the infection.
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Som
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Anulekha
authors.initials: A
authors.lastname: De
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Gourab Dutta
authors.initials: GD
authors.lastname: Banik
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Maity
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Chiranjit
authors.initials: C
authors.lastname: Ghosh
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Mithun
authors.initials: M
authors.lastname: Pal
authors.affiliation: 1] Department of Gastroenterology, AMRI Hospital, Salt Lake City, JC-16 &17, Kolkata 700098, India [2] Department of Gastroenterology, Ruby General Hospital, Kasba Golpark, E. M. Bypass Kolkata, West Bengal 700 107, India.
authors.forename: Sunil B
authors.initials: SB
authors.lastname: Daschakraborty
authors.affiliation: Department of Gastroenterology, AMRI Hospital, Salt Lake City, JC-16 &17, Kolkata 700098, India.
authors.forename: Sujit
authors.initials: S
authors.lastname: Chaudhuri
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Subhra
authors.initials: S
authors.lastname: Jana
authors.affiliation: 1] Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India [2] Thematic Unit of Excellence on Nanodevice Technology, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata 700098, India.
authors.forename: Manik
authors.initials: M
authors.lastname: Pradhan
mesh_terms: D000328:Adult; D001944:Breath Tests; D002245:Carbon Dioxide / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D016022:Case-Control Studies; D045853:Exhalation; D005260:Female; D005947:Glucose / Q000378:metabolism; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D010103:Oxygen Isotopes / Q000378:metabolism*; D012372:ROC Curve
chemical_list: D002247:Carbon Isotopes; D010103:Oxygen Isotopes; D002245:Carbon Dioxide; D005947:Glucose