title: Activation of Pathogenesis-related Genes by the Rhizobacterium, Bacillus sp. JS, Which Induces Systemic Resistance in Tobacco Plants.
abstract: Plant growth promoting rhizobacteria (PGPR) are known to confer disease resistance to plants. Bacillus sp. JS demonstrated antifungal activities against five fungal pathogens in in vitro assays. To verify whether the volatiles of Bacillus sp. JS confer disease resistance, tobacco leaves pre-treated with the volatiles were damaged by the fungal pathogen, Rhizoctonia solani and oomycete Phytophthora nicotianae. Pre-treated tobacco leaves had smaller lesion than the control plant leaves. In pathogenesis-related (PR) gene expression analysis, volatiles of Bacillus sp. JS caused the up-regulation of PR-2 encoding β-1,3-glucanase and acidic PR-3 encoding chitinase. Expression of acidic PR-4 encoding chitinase and acidic PR-9 encoding peroxidase increased gradually after exposure of the volatiles to Bacillus sp. JS. Basic PR-14 encoding lipid transfer protein was also increased. However, PR-1 genes, as markers of salicylic acid (SA) induced resistance, were not expressed. These results suggested that the volatiles of Bacillus sp. JS confer disease resistance against fungal and oomycete pathogens through PR genes expression.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan ; Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Ji-Seong
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan ; Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Jeongeun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea.
authors.forename: Chan-Hui
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Su Young
authors.initials: SY
authors.lastname: Woo
authors.affiliation: Department of Biological and Environmental Science, Dongguk University, Seoul 100-715, Korea.
authors.forename: Hoduck
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Sang-Gyu
authors.initials: SG
authors.lastname: Seo
authors.affiliation: Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Kim
keywords: antifungal activities; disease resistance; plant growth promoting rhizobacteria