title: Inbreeding compromises host plant defense gene expression and improves herbivore survival.
abstract: Inbreeding commonly occurs in flowering plants and often results in a decline in the plant's defense response. Insects prefer to feed and oviposit on inbred plants more than outbred plants--suggesting that selecting inbred host plants offers them fitness benefits. Until recently, no studies have examined the effects of host plant inbreeding on insect fitness traits such as growth and dispersal ability. In a recent article, we documented that tobacco hornworm (Manduca sexta L.) larvae that fed on inbred horsenettle (Solanum carolinense L.) plants exhibited accelerated larval growth and increased adult flight capacity compared to larvae that fed on outbred plants. Here we report that M. sexta mortality decreased by 38.2% when larvae were reared on inbred horsenettle plants compared to larvae reared on outbreds. Additionally, inbred plants showed a notable reduction in the average relative expression levels of lipoxygenease-D (LoxD) and 12-oxophytodienoate reductase-3 (OPR3), two genes in the jasmonic acid signaling pathway that are upregulated in response to herbivore damage. Our study presents evidence that furthers our understanding of the biochemical mechanism responsible for differences in insect performance on inbred vs. outbred host plants.
authors.affiliation: a Department of Biology; The Pennsylvania State University ; University Park, PA USA.
authors.forename: Scott L
authors.initials: SL
authors.lastname: Portman
authors.forename: Rupesh R
authors.initials: RR
authors.lastname: Kariyat
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Johnston
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Stephenson
authors.forename: James H
authors.initials: JH
authors.lastname: Marden
mesh_terms: D000818:Animals; D015870:Gene Expression; D060434:Herbivory; D007178:Inbreeding; D007814:Larva / Q000502:physiology; D008084:Lipoxygenase / Q000378:metabolism; D018402:Manduca / Q000502:physiology*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D032322:Solanum / Q000235:genetics*
chemical_list: D008084:Lipoxygenase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase
keywords: LOX; Manduca sexta; OPR3; Solanum carolinense; gene expression; horsenettle; inbreeding; survival