title: Tailoring the properties and functions of phosphate/silk/Ag/chitosan scaffolds.
abstract: Two novel silk composites of phosphatic phases with nanosilver/chitosan having enhanced biocompatibility were achieved. Hydroxyapatite and octa calcium phosphates were synthesized in situ within silk fibroin/chitosan/nanosilver composites recently studied. Thermo-gravimetric analysis (TGA) and Differential Scanning Calorimetry (DSC) verified their thermal behavior. The structural aspects were characterized applying X-ray diffraction analysis (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscope (FESEM) with EDAX. Additionally X-ray Photoelectron Spectroscopy (XPS) and Fourier Transform Infrared spectroscopy (FTIR) were applied. Mercury porosimeter was used to verify the pore size distribution. The in vitro degradation was followed in D-MEM for 48 h in a cumulative manner for five successive periods. Biochemical analyses of Ca, P and total protein using relevant chemical kits and atomic absorption for silver were performed. ANOVA statistics was carried out. Phosphatic crystalline phases along with the presence of silk, chitosan and nano-silver were developed. The diameters of hydroxyapatite and octa calcium phosphate particles were ~8-17 nm and 15-22 nm respectively. Comparatively higher degradation of Octa composite possessing higher porosity proved in turn more osteoinduction with in situ apatitic development.
authors.affiliation: National Research Centre, Biomaterials Department, Elbehoos St. Dokki, Giza, Egypt. Electronic address: nrcfifi@yahoo.com.
authors.forename: Wafa I
authors.initials: WI
authors.lastname: Abdel-Fattah
authors.affiliation: Faculty of Science, Physics Department, Ain Shams University, Cairo, Egypt. Electronic address: ab_m_sallam@yahoo.com.
authors.forename: Abdel Sattar M
authors.initials: AS
authors.lastname: Sallam
authors.affiliation: High Institute of Engineering, Physics Department, El-Shorouk Academy, Cairo, Egypt. Electronic address: amdiabdr55@yahoo.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Diab
authors.affiliation: National Research Centre, Biomaterials Department, Elbehoos St. Dokki, Giza, Egypt. Electronic address: wafaa_ghareeeb@yahoo.com.
authors.forename: Ghareib W
authors.initials: GW
authors.lastname: Ali
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D012831:Bombyx; D002130:Calcium Phosphates / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D016062:Porosity; D047011:Silk / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D010710:Phosphates; D047011:Silk; C022045:octacalcium phosphate; D012834:Silver; D005349:Fibroins; D048271:Chitosan; D017886:Durapatite
keywords: D-MEM; FESEM/EDAX; Hg-porosimeter; Hydroxyapatite vs octacalcium phosphate; Silk protein/chitosan/nanosilver; XPS-deconvolution