title: Anti-cancer studies of noble metal nanoparticles synthesized using different plant extracts.
abstract: Biofunctionalized gold and silver nanoparticles synthesized using different plant extracts of guava and clove in vitro anti-cancer efficacy against four different cancer cell lines human colorectal adenocarcinoma, human kidney, human chronic myelogenous, leukemia, bone marrow, and human cervix have been studied and reported. The present experimental study suggests that flavonoids functionalized gold nanoparticles synthesized using aqueous clove buds extract are more potential than guava leaf extract towards anti-cancer activities. The microscopic and 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay infer that the functionalized irregular shaped gold nanoparticles synthesized with aqueous clove bud extract showed a satisfactory anti-cancer effect on all the cell lines. The silver nanoparticles synthesized using same extracts are devoid of anti-cancer activity. The XTT assay revealed dose-dependent cytotoxicity to cancer cell lines. The study revealed that the free radicals generated by gold nanoparticles are responsible for anti-cancer effect. To confirm the free-radical scavenging efficacy of gold nanoparticle, nitric oxide assay is followed. We observed that the gold nanoparticles swabbed the free radicals in dose-dependent manner. With continued improvements, these nanoparticles may prove to be potential anti-cancer agents.
authors.affiliation: HKES Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences, Sedam Road, Gulbarga-585105, Karnataka, India.
authors.forename: Deshpande
authors.initials: D
authors.lastname: Raghunandan
authors.affiliation: Materials Chemistry Laboratory, Department of Material Science, Gulbarga University, Gulbarga, 585106 Karnataka, India.
authors.forename: Bhat
authors.initials: B
authors.lastname: Ravishankar
authors.affiliation: Materials Chemistry Laboratory, Department of Material Science, Gulbarga University, Gulbarga, 585106 Karnataka, India.
authors.forename: Ganachari
authors.initials: G
authors.lastname: Sharanbasava
authors.affiliation: Materials Chemistry Laboratory, Department of Material Science, Gulbarga University, Gulbarga, 585106 Karnataka, India.
authors.forename: D Bedre
authors.initials: DB
authors.lastname: Mahesh
authors.affiliation: Periferal Cancer Institute, Sedam Road, Gulbarga-585105, Karnataka, India.
authors.forename: Vasanth
authors.initials: V
authors.lastname: Harsoor
authors.affiliation: Sri Krupa institute of Pharmaceutical Sciences, Village Velkatta, Siddipet-502277, Medak, Andhra Pradesh India.
authors.forename: Manjunath S
authors.initials: MS
authors.lastname: Yalagatti
authors.affiliation: CM College of Pharmacy, Maisammaguda, Dulapally, Hyderabad-500014, Andhra Pradesh India.
authors.forename: M
authors.initials: M
authors.lastname: Bhagawanraju
authors.affiliation: Materials Chemistry Laboratory, Department of Material Science, Gulbarga University, Gulbarga, 585106 Karnataka, India.
authors.forename: A
authors.initials: A
authors.lastname: Venkataraman
keywords: Anti-cancer agent; Cell lines; Flavonoid functionalized gold and silver nanoparticles; In vitro activity; XTT assay