title: Effects of Lycium barbarum extract on production and immunomodulatory activity of the extracellular polysaccharopeptides from submerged fermentation culture of Coriolus versicolor.
abstract: Polysaccharopeptides (PSPs) from Coriolus versicolor have been used as immunomodulatory and anticancer agents. However, most studies have concentrated on the mycelial PSPs and not those in the fermented broth. On the other hand, Lycium barbarum fruit has been used as a traditional Chinese herbal medicine for two millennia. Its extract contains various nutrients, minerals, and also polysaccharide-protein complexes, which are proven to be bioactive. Herein we report the effects of L. barbarum fruit extract on the mycelial growth and extracellular PSP (ePSP) production of C. versicolor LH1 by using a submerged fermentation process in 20l fermenters. Fermentation production of C. versicolor biomass and its ePSP were augmented in the presence of L. barbarum extract. The ePSP such obtained differs from those obtained with normal culture medium in terms of simple sugar composition and protein content but shows similar overall chemical structures as analyzed by Fourier transformed infrared spectroscopy. Moreover, the ePSP from C. versicolor cultured with supplementary L. barbarum extract exhibits significant immunomodulatory activity as judged by its effects on the production of nitric oxide and several cytokines by murine RAW264.7 macrophages.
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, Changhua County 51591, Taiwan.
authors.forename: Fang-Yi
authors.initials: FY
authors.lastname: Lin
authors.affiliation: Department of Bioresources, Da-Yeh University, Changhua County 51591, Taiwan; Institute of Biotechnology and Department of Life Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Yiu-Kay
authors.initials: YK
authors.lastname: Lai
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, Changhua County 51591, Taiwan.
authors.forename: Hao-Chen
authors.initials: HC
authors.lastname: Yu
authors.affiliation: Department of Food Nutrition, Chung-Hwa University of Medical Technology, Tainan Hsien, 71703, Taiwan.
authors.forename: Nan-Yin
authors.initials: NY
authors.lastname: Chen
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, Changhua County 51591, Taiwan.
authors.forename: Chi-Yue
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Department of Bioscience Technology, Chang-Jung Christian University, Tainan 71101, Taiwan; Department of Medical Education and Research, Changhua Christian Hospital, Changhua 50006, Taiwan.
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Lo
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, Changhua County 51591, Taiwan. Electronic address: th4420@gmail.com.
authors.forename: Tai-Hao
authors.initials: TH
authors.lastname: Hsu
keywords: Coriolus versicolor; Extracellular polysaccharopeptides; Immunomdulatory activity; Lycium barbarum extract; Macrophages