title: Synergistic Effects of SDF-1α and BMP-2 Delivery from Proteolytically Degradable Hyaluronic Acid Hydrogels for Bone Repair.
abstract: In order to achieve bone repair, bone morphogenetic protein-2 (BMP-2) is typically delivered in non-physiological doses and can result in significant adverse side effects. To reduce the amount of BMP-2 necessary for bone formation, we delivered a known chemokine (stromal cell derived factor-1α, SDF-1α) in combination with BMP-2 using proteolytically degradable hydrogels. A critical-sized calvarial defect was used to determine the effect of biomolecule delivery on bone formation in vivo. The treatment group with combined SDF-1α and BMP-2 hydrogel delivery showed significantly higher bone formation when compared to hydrogels loaded with the same BMP-2 or SDF-1α concentrations alone, suggesting the combined delivery of both biomolecules synergistically improves osteogenesis.
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 S 33rd St, Philadelphia, Pennsylvania, 19104, USA.
authors.forename: Julianne L
authors.initials: JL
authors.lastname: Holloway
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 S 33rd St, Philadelphia, Pennsylvania, 19104, USA.
authors.forename: Henry
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 S 33rd St, Philadelphia, Pennsylvania, 19104, USA.
authors.forename: Reena
authors.initials: R
authors.lastname: Rai
authors.affiliation: Departments of Small Animal Clinical Sciences and Physiology, Michigan State University, 736 Wilson Rd, East Lansing, Michigan, 48824, USA.
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Hankenson
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 S 33rd St, Philadelphia, Pennsylvania, 19104, USA. burdick2@seas.upenn.edu.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Burdick
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D054377:Chemokine CXCL12 / Q000008:administration & dosage* / Q000494:pharmacology; D004357:Drug Synergism; D006820:Hyaluronic Acid; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D059748:Proteolysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D020100:Hydrogels; D006820:Hyaluronic Acid
keywords: bone morphogenetic protein; bone tissue engineering; drug delivery; hyaluronic acid; hydrogel; stromal cell-derived factor-1α