title: Synergistic action of laccases from Trametes hirsuta Bm2 improves decolourization of indigo carmine.
abstract: Laccase isoenzymes (LacI,II,III) produced on wheat bran from Trametes hirsuta were partially purified through anion exchange chromatography. The three isoenzymes had the same MW of 65 kDa, and their main physico-chemical properties were studied. As single isoenzymes, laccases were unable to decolourize dye. Among several mediators evaluated, syringaldehyde was the most effective in dye decolourization (100%). A remarkable increase in dye decolourization was observed when LacI, II, III in mixture or crude enzyme were added to the reaction system, indicating that the laccases acted synergistically.
  
  
  CONCLUSIONS
  Laccases have a great potential of application in bioremediation processes. White rot fungi produces several laccase isoenzymes and many of them have been purified and characterized. However, the additive or synergic action between laccase isoenzymes in dye decolourization has not yet been described. Such studies will help to better understand their action and to improve the process with isoenzymes mixtures. This study showed synergistic action between isoenzymes laccases produced by Trametes hirsuta Bm2 during decolourization of indigo carmine.
authors.affiliation: Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Mérida, Yucatán, México.
authors.forename: P
authors.initials: P
authors.lastname: Zapata-Castillo
authors.affiliation: Centro de Tecnología Enzimática, Facultad de Agronomía, Universidad de Matanzas, Matanzas, Cuba.
authors.forename: L
authors.initials: L
authors.lastname: Villalonga-Santana
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán. A.C., Mérida, Yucatán, México.
authors.forename: I
authors.initials: I
authors.lastname: Islas-Flores
authors.affiliation: Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Mérida, Yucatán, México.
authors.forename: G
authors.initials: G
authors.lastname: Rivera-Muñoz
authors.affiliation: Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Mérida, Yucatán, México.
authors.forename: W
authors.initials: W
authors.lastname: Ancona-Escalante
authors.affiliation: Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Mérida, Yucatán, México.
authors.forename: S
authors.initials: S
authors.lastname: Solís-Pereira
mesh_terms: D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry; D004043:Dietary Fiber / Q000382:microbiology; D007203:Indigo Carmine / Q000737:chemistry*; D042845:Laccase / Q000378:metabolism*; D008969:Molecular Sequence Data; D055454:Trametes / Q000201:enzymology*; D018505:Waste Management / Q000379:methods*; D062065:Wastewater
chemical_list: D004396:Coloring Agents; D004043:Dietary Fiber; D007203:Indigo Carmine; D042845:Laccase; D062065:Wastewater
keywords: Trametes hirsuta; dye decolourization; isoenzymes; laccase; purification