title: Aurora kinases are essential for PKC-induced invasion and matrix metalloproteinase-9 expression in MCF-7 breast cancer cells.
abstract: The Aurora kinase family of serine/threonine kinases are known to be crucial for cell cycle control. Aurora kinases are considered a target of anticancer drugs. However, few studies have assessed the effect of Aurora kinases in breast cancer. In the present study, to determine whether Aurora kinases play a role in oncogenic actions of protein kinase C (PKC), we investigated the effect of Aurora kinases on PKC-induced invasion and MMP-9 expression using breast cancer cells. Treatment of MCF-7 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced the upregulation and phosphorylation of Aurora kinases via the MAPK signaling pathway. Moreover, the inhibition of Aurora kinases by their siRNAs and inhibitors suppressed TPA-induced cell invasion and expression of MMP-9 by inhibiting the activation of NF-κB/AP-1, major transcription factors for MMP-9 expression in MCF-7 cells. These results suggested that Aurora kinases mediate PKC-MAPK signal to NF-κB/AP-1 with increasing MMP-9 expression and invasion of MCF-7 cells. To the best of our knowledge, this is the first study to show that Aurora kinases are key molecules in PKC-induced invasion in breast cancer cells.
authors.affiliation: Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 560‑182, Republic of Korea.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Noh
authors.affiliation: Department of Oral Biochemistry and Institute of Biomaterials-Implant, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea.
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 560‑182, Republic of Korea.
authors.forename: On-Yu
authors.initials: OY
authors.lastname: Hong
authors.affiliation: Department of Surgery and Research Institute of Clinical Medicine, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea.
authors.forename: Sung Hoo
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Department of Surgery and Research Institute of Clinical Medicine, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea.
authors.forename: Hyun Jo
authors.initials: HJ
authors.lastname: Youn
authors.affiliation: Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 560‑182, Republic of Korea.
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
mesh_terms: D064096:Aurora Kinase A / Q000096:biosynthesis* / Q000235:genetics; D064107:Aurora Kinase B / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D016328:NF-kappa B; D064096:Aurora Kinase A; D064107:Aurora Kinase B; D011493:Protein Kinase C; D020929:Mitogen-Activated Protein Kinase Kinases; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate