title: MiR-615 inhibited cell proliferation and cell cycle of human breast cancer cells by suppressing of AKT2 expression.
abstract: MicroRNAs (miRNAs) have emerged as important regulators that potentially play critical roles in various biological processes. Previous studies have shown that miR-615 regulates proliferation and apoptosis in many types of cancers. The biological function of this microRNA in breast cancer remains largely unexplored. In the present study, we found that miR-615 expression was markedly downregulated in breast cancer tissues and breast cancer cells. The enforced expression of miR-615 was able to inhibite the proliferation and anchorage-independent growth of breast cancer cells, while miR-615-in showed the opposite effect. Bioinformatics analysis further revealed AKT2, a putative tumor promoter as a potential target of miR-615. Ectopic expression of miR-615 led to downregulation of AKT2 protein, which resulted in the upregulation of p27 and p21 and the downregulation of cyclin D1. In sum, these results suggest that miR-615 represents a potential anti-onco-miR and participates in breast cancer carcinogenesis by suppressing AKT2 expression.
authors.affiliation: Department of Surgical Oncology, Cangzhou Centerl Hospital Cangzhou, Hebei Province, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Radiology, Hebei province Cangzhou Hospital of integrated traditional and Western Medicine (Cangzhou NO. 2 Hospital) Cangzhou, Hebei Province, P. R. China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, Cangzhou Centerl Hospital Cangzhou, Hebei Province, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, Cangzhou Centerl Hospital Cangzhou, Hebei Province, P. R. China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Surgical Oncology, Cangzhou Centerl Hospital Cangzhou, Hebei Province, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
keywords: AKT2; breast cancer; cell cycle; cell proliferation; miR-615