title: [The optimization of the nitric oxide quantitative analysis for its determination in the cultural medium of mammalian cell culture].
abstract: The protocol for the quantitative analysis of nitric oxide as nitrite-ion suitable for determination of its production by a mammalian cell culture was developed. The optimal results were obtained using microvolume-adjusted Griess method after the preliminary reduction of NO3- to NO2- with non-activated cadmium. The protocol was verified on a rat glioma C6 cell culture. The developed method may be used for the nitric oxide determination in 96-well and 48-well microplates; the detection limit is 2.1 ± 0.1 μM for NO2- and 2.9 ± 0.1 μM for NO3-.
authors.forename: M G
authors.initials: MG
authors.lastname: Akimov
authors.forename: E V
authors.initials: EV
authors.lastname: Fomina-Ageeva
authors.forename: V V
authors.initials: VV
authors.lastname: Bezuglov
mesh_terms: D000818:Animals; D002104:Cadmium / Q000737:chemistry*; D045744:Cell Line, Tumor; D009566:Nitrates / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D009566:Nitrates; D002104:Cadmium; D009569:Nitric Oxide