title: Antioxidative Activity of Flavonoids from Abrus cantoniensis against Ethanol-Induced Gastric Ulcer in Mice.
abstract: The present study investigated the flavonoids from Abrus cantoniensis against ethanol-induced gastric ulcers in mice. The flavonoids from A. cantoniensis were extracted with ethanol and purified by macroporous resin and polyamide. The 2,2-diphenyl-1-picrylhydrazyl assay was used to measure the antioxidative activities in vitro. The ethanol-induced ulcer mouse model was used to evaluate the gastroprotective activities of the flavonoids from A. cantoniensis. In addition, a method was established to ensure accuracy for animal ulcer evaluation. The flavonoids from A. cantoniensis showed a strong free radical scavenging capacity with an IC50 of 43.83 µg/mL in the 2,2-diphenyl-1-picrylhydrazyl assay. At doses between 28.16-112.67 mg/kg, the flavonoids conspicuously reduced the ulcer index in ethanol-induced mice (p<0.001). Significant differences were found in the levels of superoxide dismutase, catalase, glutathione, and myeloperoxidase in the stomach tissues between the flavonoids from the A. cantoniensis groups and the ethanol control group. The gastroprotective effect of the flavonoids from A. cantoniensis could be due to its antioxidative activity of the defensive mechanism. The data revealed that the flavonoids from A. cantoniensis could be a potential therapeutic agent for gastric ulcer prevention and treatment.
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Zi-Jing
authors.initials: ZJ
authors.lastname: Song
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Dai
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Su-Li
authors.initials: SL
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, JS, China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Chang-Run
authors.initials: CR
authors.lastname: Guo
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Jiao-Ying
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA.
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA.
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D029912:Abrus / Q000737:chemistry*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D000431:Ethanol / Q000009:adverse effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D009195:Peroxidase / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D005419:Flavonoids; D000431:Ethanol; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione