title: Mineral and Skeletal Homeostasis Influence the Manner of Bone Loss in Metabolic Osteoporosis due to Calcium-Deprived Diet in Different Sites of Rat Vertebra and Femur.
abstract: Rats fed calcium-deprived diet develop osteoporosis due to enhanced bone resorption, secondary to parathyroid overactivity resulting from nutritional hypocalcemia. Therefore, rats provide a good experimental animal model for studying bone modelling alterations during biochemical osteoporosis. Three-month-old Sprague-Dawley male rats were divided into 4 groups: (1) baseline, (2) normal diet for 4 weeks, (3) calcium-deprived diet for 4 weeks, and (4) calcium-deprived diet for 4 weeks and concomitant administration of PTH (1-34) 40 µg/Kg/day. Histomorphometrical analyses were made on cortical and trabecular bone of lumbar vertebral body as well as of mid-diaphysis and distal metaphysis of femur. In all rats fed calcium-deprived diet, despite the reduction of trabecular number (due to the maintenance of mineral homeostasis), an intense activity of bone deposition occurs on the surface of the few remaining trabeculae (in answering to mechanical stresses and, consequently, to maintain the skeletal homeostasis). Different responses were detected in different sites of cortical bone, depending on their main function in answering mineral or skeletal homeostasis. This study represents the starting point for work-in-progress researches, with the aim of defining in detail timing and manners of evolution and recovery of biochemical osteoporosis.
authors.affiliation: Dipartimento di Scienze Biomediche, Metaboliche e Neuroscienze-Sezione di Morfologia Umana, Università di Modena e Reggio Emilia, Istituti Anatomici, 41124 Modena, Italy.
authors.forename: Marzia
authors.identifier: 0000-0003-0280-3598
authors.initials: M
authors.lastname: Ferretti
authors.affiliation: Dipartimento di Scienze Biomediche, Metaboliche e Neuroscienze-Sezione di Morfologia Umana, Università di Modena e Reggio Emilia, Istituti Anatomici, 41124 Modena, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cavani
authors.affiliation: Dipartimento di Scienze Biomediche, Metaboliche e Neuroscienze-Sezione di Morfologia Umana, Università di Modena e Reggio Emilia, Istituti Anatomici, 41124 Modena, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Smargiassi
authors.affiliation: Laboratorio di Patologia Clinica Endocrinologia, Dipartimento Interaziendale ad Attività Integrata Medicina di Laboratorio ed Anatomia Patologica, AUSL Modena, 41126 Modena, Italy.
authors.forename: Laura
authors.identifier: 0000-0001-7154-189X
authors.initials: L
authors.lastname: Roli
authors.affiliation: Dipartimento di Scienze Biomediche, Metaboliche e Neuroscienze-Sezione di Morfologia Umana, Università di Modena e Reggio Emilia, Istituti Anatomici, 41124 Modena, Italy.
authors.forename: Carla
authors.identifier: 0000-0003-0587-0112
authors.initials: C
authors.lastname: Palumbo
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000503:physiopathology*; D001862:Bone Resorption / Q000378:metabolism* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism*; D004032:Diet; D005269:Femur / Q000378:metabolism / Q000503:physiopathology; D006706:Homeostasis; D006801:Humans; D008159:Lumbar Vertebrae / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D010024:Osteoporosis / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats
chemical_list: D002118:Calcium