title: Shell Cross-Linked Polymeric Micelles as Camptothecin Nanocarriers for Anti-HCV Therapy.
abstract: A suitable carrier for camptothecin to act as therapy against the hepatitis C virus is presented. The carrier relies on an amphiphilic hybrid dendritic-linear-dendritic block copolymer, derived from pluronic F127 and bis-MPA dendrons, that forms micelles in aqueous solution. The dendrons admit the incorporation of multiple photoreactive groups that allow the clean and effective preparation of covalently cross-linked polymeric micelles (CLPM), susceptible of loading hydrophilic and lipophilic molecules. Cell-uptake experiments using a newly designed fluorophore, derived from rhodamine B, demonstrate that the carrier favors the accumulation of its cargo within the cell. Furthermore, loaded with camptothecin, it is efficient in fighting against the hepatitis C virus while shows lower cytotoxicity than the free drug.
authors.affiliation: Departamento de Química Orgánica, Instituto de Ciencia de Materiales de Aragón (ICMA) - Universidad de Zaragoza-CSIC, 50009, Zaragoza, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Jiménez-Pardo
authors.affiliation: Instituto de Investigaciones Sanitarias de Aragon (IIS-Aragon), 50009, Zaragoza, Spain.|Instituto de Ciencias de la Salud (IACS), 50009, Zaragoza, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: González-Pastor
authors.affiliation: Departamento de Química Orgánica, Instituto de Nanociencia de Aragón, Universidad de Zaragoza, 50018, Zaragoza, Spain.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Lancelot
authors.affiliation: Instituto de Investigaciones Sanitarias de Aragon (IIS-Aragon), 50009, Zaragoza, Spain.|Instituto de Ciencias de la Salud (IACS), 50009, Zaragoza, Spain.|Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain.|Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit IQFR-CSIC-BIFI, Universidad de Zaragoza, 50018, Zaragoza, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Claveria-Gimeno
authors.affiliation: Instituto de Investigaciones Sanitarias de Aragon (IIS-Aragon), 50009, Zaragoza, Spain.|Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit IQFR-CSIC-BIFI, Universidad de Zaragoza, 50018, Zaragoza, Spain.|Fundación ARAID, Government of Aragon, 50018, Zaragoza, Spain.
authors.forename: Adrián
authors.initials: A
authors.lastname: Velázquez-Campoy
authors.affiliation: Instituto de Investigaciones Sanitarias de Aragon (IIS-Aragon), 50009, Zaragoza, Spain.|Instituto de Ciencias de la Salud (IACS), 50009, Zaragoza, Spain.|Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain.|Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit IQFR-CSIC-BIFI, Universidad de Zaragoza, 50018, Zaragoza, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Abian
authors.affiliation: Departamento de Química Orgánica, Instituto de Ciencia de Materiales de Aragón (ICMA) - Universidad de Zaragoza-CSIC, 50009, Zaragoza, Spain. bros@unizar.es.
authors.forename: M Blanca
authors.initials: MB
authors.lastname: Ros
authors.affiliation: Departamento de Química Orgánica, Instituto de Ciencia de Materiales de Aragón (ICMA) - Universidad de Zaragoza-CSIC, 50009, Zaragoza, Spain. tsierra@unizar.es.
authors.forename: Teresa
authors.initials: T
authors.lastname: Sierra
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002151:Calorimetry; D002166:Camptothecin / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004705:Endocytosis / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D016174:Hepacivirus / Q000187:drug effects*; D006801:Humans; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D020442:Poloxamer / Q000138:chemical synthesis / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008823:Micelles; D011108:Polymers; D013501:Surface-Active Agents; D020442:Poloxamer; D002166:Camptothecin
keywords: anti-HCV; camptothecin; cross-linked micelles; dendrimers; pluronic