title: Hydrogen sulfide mediated cascade reaction forming an iminocoumarin: applications in fluorescent probe development and live-cell imaging.
abstract: The study on a fluorescent probe that undergoes a H2S mediated cascade reaction to form an iminocoumarin fluorophore is reported. The probe features better water solubility and fast sensing time (t1/2 = 6.1 min and response time = 24 min) as key advances compared to the reported probe that works on a similar mechanism. The sensing mechanism of the probe was demonstrated by mass spectrometric, HPLC titration and FT-IR titration methods. H2S sensing by the probe was characterized by a 31-fold fluorescence enhancement and alimit of detection of 169 nM. Application of the probe was demonstrated by imaging of H2S in live cells.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Pune, India. ptalukdar@iiserpune.ac.in.
authors.forename: Pratryush Kumar
authors.initials: PK
authors.lastname: Mishra
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Saha
authors.forename: Pinaki
authors.initials: P
authors.lastname: Talukdar
mesh_terms: D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin; D006862:Hydrogen Sulfide