title: Visfatin promotes osteosarcoma cell migration and invasion via induction of epithelial-mesenchymal transition.
abstract: Visfatin is considered to be a biomarker in various types of cancers. However, no evidence has been reported for the direct effect of visfatin on osteosarcoma cell metastasis. The aims of the present study were to investigate the influence of visfatin on the migration and invasion of osteosarcoma cells and clarify the underlying mechanism. The expression levels of epithelial-mesenchymal transition (EMT) markers, as well as the transcriptional factor Snail-1, were first detected at both the protein and mRNA levels in U2OS osteosarcoma cells after stimulation of visfatin. Then the expression of NF-κB (p65) was detected by western blot analysis, and siRNA of Snail-1 and inhibitor of NF-κB were used to investigate the effect of visfatin. Finally, migration and invasion of the cells were detected respectively by scratch wound healing and transwell assays. Visfatin downregulated E-cadherin and upregulated N-cadherin in concentration- and time-dependent manners at the protein and mRNA levels. The expression of Snail-1 was also upregulated. Moreover, visfatin also promoted the nuclear translocation of the NF-κB pathway. Administration of siRNA of Snail-1 and the inhibitor BAY11-7082 validated the roles of Snail-1 and NF-κB in the visfatin-induced regulation of EMT markers. Migration and invasion of U2OS osteosarcoma cells were promoted following the application of visfatin. These results demonstrated that visfatin enhances the migration and invasion of osteosarcoma cells via the NF-κB/Snail-1/EMT pathway.
authors.affiliation: Department of Anatomy, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Department of Orthopedics, People's Hospital of Linyi City, Linyi, Shandong, P.R. China.
authors.forename: Changying
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Sports Medicine, Yantaishan Hospital, Yantai, Shandong, P.R. China.
authors.forename: Xiujiang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Central Hospital of Zibo, Zibo, Shandong, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Central Hospital of Zibo, Zibo, Shandong, P.R. China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: Department of Cardiology, Central Hospital of Zibo, Zibo, Shandong, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D016207:Cytokines / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D016328:NF-kappa B; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human