title: Comparison of capillary electrophoresis and high performance liquid chromatography for detection and quantification of hemoglobin New York.
abstract: BACKGROUND
  Hemoglobin (Hb) New York [β113 (G15) Val→Glu, GTG→GAG], also known as Hb Kaohsiung, is one of the most common Hb variants in South China. Currently, most used screening methods for hemoglobinopathies in South China are high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). However, there is no study comparing the performance of CE and HPLC in the detection of Hb New York.
  
  
  METHODS
  In total 15 samples (including 13 adult blood samples and 2 cord blood samples) with heterozygous Hb New York were analyzed by CE and HPLC. Levels of Hb New York, HbA2, Hb, mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were compared within the two groups.
  
  
  RESULTS
  All 15 cases (100%) were detected by CE, whereas none was detected by HPLC. Mean values of Hb New York and HbA2 in adult heterozygous group were (43.82±2.47)% and (2.85±0.31)%, respectively; mean values for cord blood group were (7.95±0.78)% and (0.30±0.14)%.
  
  
  CONCLUSIONS
  CE allows the detection of Hb New York, while this variant is not separated from Hb A on HPLC. CE may be the preferred method for hemoglobinopathy screening in areas with high prevalence of Hb New York.
authors.forename: Li
authors.initials: L
authors.lastname: You-Qiong
authors.forename: Huang
authors.initials: H
authors.lastname: Hui-Ping
authors.forename: Chen
authors.initials: C
authors.lastname: Zhi-Zhong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Lin
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.forename: Qin
authors.initials: Q
authors.lastname: Gui-Fang
authors.forename: Mo
authors.initials: M
authors.lastname: Yun
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn
chemical_list: D006455:Hemoglobins, Abnormal; C032181:hemoglobin New York