title: Decreased miR-154 expression and its clinical significance in human colorectal cancer.
abstract: BACKGROUND
  miRNA-154 (miR-154) has been identified as a tumor suppressor in several types of human cancers. However, its clinical significance in colorectal cancer (CRC) is still unclear. The aim of this study was to analyze the association of miR-154 expression with clinicopathologic features and prognosis in CRC patients.
  
  
  METHODS
  Quantitative RT-PCR was performed to evaluate miR-154 levels in 169 pairs of CRC specimens and adjacent noncancerous tissues. Then, the associations of miR-154 expression with clinicopathological factors or survival of patients suffering CRC were determined.
  
  
  RESULTS
  The expression levels of miR-154 in CRC tissues were significantly lower than those in corresponding noncancerous tissues (P < 0.001). Decreased miR-154 expression was significantly associated with large tumor size, positive lymph node metastasis, and advanced clinical stage. Moreover, the univariate analysis demonstrated that CRC patients with low miR-154 expression had poorer overall survival (P = 0.006). The multivariate analysis identified low miR-154 expression as an independent predictor of poor survival.
  
  
  CONCLUSIONS
  These findings suggested that miR-154 downregulation may be associated with tumor progression of CRC, and that this miR may be an independent prognostic marker for CRC patients.
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Kai
authors.affiliation: Department of neurology, Huai'an No. 2 Hospital, Huai'an, Jiangsu Province, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qiang
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Xinxin
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Miaomiao
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China. manulinkl@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Kuailu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate; D055815:Young Adult
chemical_list: C561122:MIRN154 microRNA, human; D035683:MicroRNAs