title: Different sample types in pigs challenged with Haemophilus parasuis following two treatment schemes with tulathromycin.
abstract: This study aimed to test the efficacy of samplings for the detection of Haemophilus parasuis after metaphylactic treatment and subsequent challenge using an established model for Glässer's disease. In this model, 36 piglets were equally assigned to a negative control, a positive control, and two trial groups receiving tulathromycin 7 or 4 days prior to challenge. The piglets of three groups were challenged intratracheally with H. parasuis serovar 5. As a result, four pigs in each challenged group died or had to be euthanised within 10 days post challenge. The remaining 15 pigs of these challenged groups survived until termination of the experiment (days 14-15). All pigs were necropsied and collective swabs of serosal surfaces were tested by bacterial culture and PCR. Samples of tarsal synovial fluid and joint capsule, cerebrospinal fluid (CSF) and brain swabs were tested by PCR. A total of 22 out of the 27 challenged animals had macroscopically detectable polyserositis and all of them tested positive in the collective swab samples. Haemophilus parasuis was more frequently detected in pigs that died within the first 10 days compared to those surviving until days 14-15 (P < 0.001), and those that succumbed within 10 days showed higher positivity rates in the brain and CSF. All pigs which were positive in the CSF had detectable meningitis. At days 14-15, joint samples from 5 of the remaining 15 pigs tested positive for H. parasuis. Four of these five animals did not show any macroscopic or histological lesions in the joints. In conclusion, collective swabs were the best sample material in acute cases, whereas samples from the joints gave the best results in chronic cases. In this challenge model it was not possible to prove the metaphylactic effect of tulathromycin administered 4 and 7 days prior to infection with H. parasuis.
authors.affiliation: Clinic for Swine, Ludwig-Maximilians-University Munich , Sonnenstrasse 16, D-85764 Oberschleissheim , Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Palzer
authors.forename: Rose-Leah
authors.initials: RL
authors.lastname: Austin-Busse
authors.forename: Andrea
authors.initials: A
authors.lastname: Ladinig
authors.forename: Gyula
authors.initials: G
authors.lastname: Balka
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
authors.forename: Mathias
authors.initials: M
authors.lastname: Ritzmann
keywords: Experimental infection; Haemophilus parasuis; PCR; swab samples