title: Comparative genomic analysis of Brucella abortus vaccine strain 104M reveals a set of candidate genes associated with its virulence attenuation.
abstract: The Brucella abortus strain 104M, a spontaneously attenuated strain, has been used as a vaccine strain in humans against brucellosis for 6 decades in China. Despite many studies, the molecular mechanisms that cause the attenuation are still unclear. Here, we determined the whole-genome sequence of 104M and conducted a comprehensive comparative analysis against the whole genome sequences of the virulent strain, A13334, and other reference strains. This analysis revealed a highly similar genome structure between 104M and A13334. The further comparative genomic analysis between 104M and A13334 revealed a set of genes missing in 104M. Some of these genes were identified to be directly or indirectly associated with virulence. Similarly, a set of mutations in the virulence-related genes was also identified, which may be related to virulence alteration. This study provides a set of candidate genes associated with virulence attenuation in B.abortus vaccine strain 104M.
authors.affiliation: a Beijing Institute of Biotechnology ; Beijing , PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yu
authors.affiliation: b LanZhou Institute of Biological Products ; Lanzhou , PR China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Hui
authors.affiliation: a Beijing Institute of Biotechnology ; Beijing , PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zai
authors.affiliation: a Beijing Institute of Biotechnology ; Beijing , PR China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xu
authors.affiliation: a Beijing Institute of Biotechnology ; Beijing , PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Liang
authors.affiliation: b LanZhou Institute of Biological Products ; Lanzhou , PR China.
authors.forename: Bingxiang
authors.initials: B
authors.lastname: Wang
authors.affiliation: a Beijing Institute of Biotechnology ; Beijing , PR China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yue
authors.affiliation: a Beijing Institute of Biotechnology ; Beijing , PR China.
authors.forename: Shanhu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D002004:Brucella Vaccine / Q000235:genetics* / Q000276:immunology; D002003:Brucella abortus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity; D002006:Brucellosis / Q000382:microbiology; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods; D017385:Sequence Homology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014774:Virulence / Q000235:genetics / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D002004:Brucella Vaccine; D004269:DNA, Bacterial; D014613:Vaccines, Attenuated
keywords: Brucella abortus; comparative genomics; vaccine; virulence; virulence attenuation