title: Retrograde NGF axonal transport--motor coordination in the unidirectional motility regime.
abstract: We present a detailed motion analysis of retrograde nerve growth factor (NGF) endosomes in axons to show that mechanical tugs-of-war and intracellular motor regulation are complimentary features of the near-unidirectional endosome directionality. We used quantum dots to fluorescently label NGF and acquired trajectories of retrograde quantum-dot-NGF-endosomes with <20-nm accuracy at 32 Hz in microfluidic neuron cultures. Using a combination of transient motion analysis and Bayesian parsing, we partitioned the trajectories into sustained periods of retrograde (dynein-driven) motion, constrained pauses, and brief anterograde (kinesin-driven) reversals. The data shows many aspects of mechanical tugs-of-war and multiple-motor mechanics in NGF-endosome transport. However, we found that stochastic mechanical models based on in vitro parameters cannot simulate the experimental data, unless the microtubule-binding affinity of kinesins on the endosome is tuned down by 10 times. Specifically, the simulations suggest that the NGF-endosomes are driven on average by 5-6 active dyneins and 1-2 downregulated kinesins. This is also supported by the dynamics of endosomes detaching under load in axons, showcasing the cooperativity of multiple dyneins and the subdued activity of kinesins. We discuss the possible motor coordination mechanism consistent with motor regulation and tugs-of-war for future investigations.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California.
authors.forename: Praveen D
authors.initials: PD
authors.lastname: Chowdary
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California.
authors.forename: Daphne L
authors.initials: DL
authors.lastname: Che
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California. Electronic address: bcui@stanford.edu.
authors.forename: Bianxiao
authors.initials: B
authors.lastname: Cui
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001499:Bayes Theorem; D001696:Biomechanical Phenomena; D015536:Down-Regulation; D004398:Dyneins / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology; D016547:Kinesins / Q000378:metabolism; D051379:Mice; D008959:Models, Neurological; D020409:Molecular Motor Proteins / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D045663:Quantum Dots / Q000737:chemistry; D013269:Stochastic Processes; D013696:Temperature; D013997:Time Factors
chemical_list: D020409:Molecular Motor Proteins; D020932:Nerve Growth Factor; D004398:Dyneins; D016547:Kinesins