title: The STAT3-regulated long non-coding RNA Lethe promote the HCV replication.
abstract: BACKGROUND
  Long non-coding RNAs (lncRNAs) are a class of RNAs that do not code protein but play important roles in diverse biological processes. In recent years, with the application of high-throughput sequencing, a great deal of lncRNAs associated with virus infections have been discovered and intensively studied, but there are few studies about the relationship between lncRNAs and HCV replication.
  
  
  METHODS
  We identify that several lncRNAs can be upregulated and downregulated by phosphorylated STAT3 by using human PCR array method. And among these lncRNAs, lnc-Lethe was involved in the HCV replication. Transfection of siRNA Lethe partially blocked the replication of HCV in Huh7 cells.
  
  
  RESULTS
  In the present study, we have established that phosphorylated STAT3 can promote the HCV replication. Data also indicated that when transfected with siRNA Lethe, the expression levels of PKR, OAS and IRF1, which were all ISGs, were all up regulated.
  
  
  CONCLUSIONS
  Based on our findings from Lethe knockdown, we have identified that Lethe, which was upregulated by activated STAT3, may promoting the replication of HCV through a negative regulatory mechanism of type I IFN response.
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Kuang
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lan
authors.affiliation: Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China. Electronic address: wangxhke@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D007370:Interferon Type I; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor
keywords: HCV replication; Lethe; Long non-coding RNAs; STAT3