title: Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis.
abstract: BACKGROUND
  Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein.
  
  
  METHODS
  CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1.
  
  
  RESULTS
  Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (P < 0.001). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions (P < 0.0001) and was related to the severity of the lesions (Spearman's rho = 0.775, P = 0.0001).
  
  
  CONCLUSIONS
  Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.
authors.affiliation: Department of Pathology and IBIMER, School of Medicine, University of Granada, 18012 Granada, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: O'Valle
authors.affiliation: The Spanish Institute of Social Security (INSS), 18006 Granada, Spain.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Peregrina
authors.affiliation: Department of Pathology and IBIMER, School of Medicine, University of Granada, 18012 Granada, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Crespo-Lora
authors.affiliation: Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, 18017 Granada, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Galindo-Moreno
authors.affiliation: Plastic Surgery Department, Virgen de las Nieves University Hospital, 18014 Granada, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Roman
authors.affiliation: Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, 18017 Granada, Spain.
authors.forename: Miguel
authors.identifier: 0000-0001-6222-1341
authors.initials: M
authors.lastname: Padial-Molina
authors.affiliation: Periodontics Department, School of Dentistry, University of Granada, 18017 Granada, Spain.
authors.forename: Francisco
authors.identifier: 0000-0002-8293-2527
authors.initials: F
authors.lastname: Mesa
authors.affiliation: Department of Pathology and IBIMER, School of Medicine, University of Granada, 18012 Granada, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Aneiros-Fernandez
authors.affiliation: Department of Pathology and IBIMER, School of Medicine, University of Granada, 18012 Granada, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Aguilar
authors.affiliation: Parasitology and Biomedicine López-Neyra Institute, CSIC, 18016 Armilla, Granada, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Gonzalez-Rey
authors.affiliation: Parasitology and Biomedicine López-Neyra Institute, CSIC, 18016 Armilla, Granada, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Delgado
authors.affiliation: Orthopedic Surgery Department, San Cecilio University Hospital of Granada, 18012 Granada, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Hernandez-Cortes
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics* / Q000503:physiopathology; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism
chemical_list: C491297:MSI1 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins