title: Invariant Natural Killer T Cells Play a Role in Chemotaxis, Complement Activation and Mucus Production in a Mouse Model of Airway Hyperreactivity and Inflammation.
abstract: CD1d-restricted invariant natural killer T (iNKT) cells play a critical role in the induction of airway hyperreactivity (AHR). After intranasal alpha-galactosylceramide (α-GalCer) administration, bronchoalveolar lavage fluid (BALF) proteins from mouse lung were resolved by two-dimensional differential gel electrophoresis (2D-DIGE), and identified by tandem mass spectroscopy. A lack of iNKT cells prevented the development of airway responses including AHR, neutrophilia and the production of the proinflammatory cytokines in lungs. Differentially abundant proteins in the BALF proteome of α-GalCer-treated wild type mice included lungkine (CXCL15), pulmonary surfactant-associated protein D (SFTPD), calcium-activated chloride channel regulator 1 (CLCA1), fragments of complement 3, chitinase 3-like proteins 1 (CH3LI) and 3 (CH3L3) and neutrophil gelatinase-associated lipocalin (NGAL). These proteins may contribute to iNKT regulated AHR via several mechanisms: altering leukocyte chemotaxis, increasing airway mucus production and possibly via complement activation.
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Piia
authors.initials: P
authors.lastname: Karisola
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Maili
authors.initials: M
authors.lastname: Lehto
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Pia
authors.initials: P
authors.lastname: Kinaret
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Niina
authors.initials: N
authors.lastname: Ahonen
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Rita
authors.initials: R
authors.lastname: Haapakoski
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Minna
authors.initials: M
authors.lastname: Anthoni
authors.affiliation: RIKEN Center for Integrative Medical Sciences, Laboratory for Immune Regulation, RCAI Kanagawa, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Henrik
authors.initials: H
authors.lastname: Wolff
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Puustinen
authors.affiliation: Unit of Systems Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Harri
authors.initials: H
authors.lastname: Alenius
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D019743:Chemokines, CXC / Q000378:metabolism; D002633:Chemotaxis; D000071451:Chitinase-3-Like Protein 1; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology* / Q000502:physiology; D037102:Lectins / Q000378:metabolism; D000071068:Lipocalin-2; D054834:Lipocalins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009093:Mucus / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D000209:Acute-Phase Proteins; D019743:Chemokines, CXC; C509289:Chil1 protein, mouse; D000071451:Chitinase-3-Like Protein 1; D003176:Complement C3; C118893:Cxcl15 protein, mouse; D006023:Glycoproteins; D037102:Lectins; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D037663:Pulmonary Surfactant-Associated Protein D; C071332:Lcn2 protein, mouse; C429541:Chil3 protein, mouse; D001619:beta-N-Acetylhexosaminidases