title: Genomic characterization of two novel HIV-1 unique (CRF01_AE/B) recombinant forms among men who have sex with men in Beijing, China.
abstract: We report here two novel HIV-1 recombinant forms (CRF01_AE/B) isolated from two HIV-positive male subjects infected through homosexual contact in Beijing, China. Recombination contributes substantially to the genetic diversity of HIV-1, and is likely to occur in populations in which multiple subtypes circulate. Molecular epidemiological studies showed that subtype B, CRF01_AE, and CRF07_BC are currently cocirculating in parallel among men who have sex with men (MSM) in China, providing the opportunity for the emergence of new recombinants. Phylogenetic analysis of near full-length genome (NFLG) sequences showed that the unique recombinant forms (URFs) were composed of gene regions from CRF01_AE and subtype B. The CRF01_AE region of the recombinants clustered together with a previously described cluster 4 lineage of CRF01_AE. The B regions of both the recombinants clustered within the B strains. The two recombinants were quite similar with six breakpoints in common. These data highlight the importance of continuous surveillance of the dynamic change of HIV-1 subtypes and new recombinants among the MSM population.
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Beijing, China .
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: 2 Beijing Center for Disease Control and Prevention , Beijing, China .
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Beijing, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.affiliation: 3 Vanderbilt Institute for Global Health, Vanderbilt University School of Medicine , Nashville, Tennessee.
authors.forename: Marcia L
authors.initials: ML
authors.lastname: Kalish
authors.affiliation: 2 Beijing Center for Disease Control and Prevention , Beijing, China .
authors.forename: Hongyan
authors.initials: H
authors.lastname: Lu
authors.affiliation: 3 Vanderbilt Institute for Global Health, Vanderbilt University School of Medicine , Nashville, Tennessee.
authors.forename: Lu
authors.initials: L
authors.lastname: Yin
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Beijing, China .
authors.forename: Lingjie
authors.initials: L
authors.lastname: Liao
authors.affiliation: 3 Vanderbilt Institute for Global Health, Vanderbilt University School of Medicine , Nashville, Tennessee.
authors.forename: Han-Zhu
authors.initials: HZ
authors.lastname: Qian
authors.affiliation: 4 Department of Veterinary Medicine, University of Cambridge , Cambridge, United Kingdom .
authors.forename: Simon D W
authors.initials: SD
authors.lastname: Frost
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Beijing, China .
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: 3 Vanderbilt Institute for Global Health, Vanderbilt University School of Medicine , Nashville, Tennessee.
authors.forename: Sten H
authors.initials: SH
authors.lastname: Vermund
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Beijing, China .
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Beijing, China .
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
mesh_terms: D002681:China; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D010802:Phylogeny; D011995:Recombination, Genetic