title: Vitamin A Deficiency Impairs Induction of Oral Tolerance in Mice.
abstract: Oral tolerance is a phenomenon of induction of systemic unresponsiveness to antigens ingested by the oral route and loss of immune response. Studies have shown the importance of vitamin A in oral tolerance in vitro but not in an in vivo experimental model. Therefore, we carried out experiments to determine how vitamin A deficiency affects tolerance induction and the ability of mesenteric lymph node (MLN) CD11c(+) cells to induce regulatory T cells (Tregs). Immunological tolerance was induced by oral ovalbumin (OVA) administration in vitamin A-sufficient mice. OVA-specific antibody and cytokine production were significantly reduced. On the other hand, in vitamin A-deficient mice, both OVA-specific antibody and cytokine production were not suppressed by oral OVA administration. Regarding induction of Tregs, the conversion rate of Foxp3(+) cells from naïve CD4(+) cell by CD11c(+) cells was decreased in vitamin A-deficient mice. Our study indicates that vitamin A deficiency causes the breakdown of oral tolerance in vivo.
authors.affiliation: Department of Public Health and Applied Nutrition, Institution of Health Bioscience, The University of Tokushima Graduate School.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakamoto
authors.forename: Emi
authors.initials: E
authors.lastname: Shuto
authors.forename: Rie
authors.initials: R
authors.lastname: Tsutsumi
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamoto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nii
authors.forename: Tohru
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D018845:CD11 Antigens; D015704:CD4 Antigens; D016207:Cytokines / Q000096:biosynthesis; D051858:Forkhead Transcription Factors / Q000378:metabolism; D007108:Immune Tolerance; D007110:Immunity, Active; D008198:Lymph Nodes; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D014801:Vitamin A / Q000378:metabolism*; D014802:Vitamin A Deficiency / Q000150:complications* / Q000378:metabolism
chemical_list: D000906:Antibodies; D018845:CD11 Antigens; D015704:CD4 Antigens; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D014801:Vitamin A; D010047:Ovalbumin