title: Evaluation of store lesion in platelet obtained by apheresis compared to platelet derived from whole blood and its impact on the in vitro functionality.
abstract: Platelet units for transfusion purposes are obtained manually from whole blood or by apheresis, in an automated process. In both methods, platelets during storage present a characteristics grouped under the name "storage lesion" that are associated with adverse effects on platelet units. Oxidative stress has been claimed to be one of major causes, leading to activation and apoptosis processes affecting their post transfusion functionality. In this work, we observed an association between apheresis and a reduced presence of oxidative stress and better results in functional markers in stored platelets, compared to manually obtained platelets. Then, apheresis which would ensure a greater number of functional platelets during the 5 days of storage, compared to concentrates obtained from whole blood.
authors.affiliation: Blood Bank and Transfusion Service, Foundation Valle of Lili Clinic, Cali, Colombia; Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Universidad de Talca, Talca, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Quintero
authors.affiliation: Blood Bank, Santa María Clinic, Santiago de Chile, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Núñez
authors.affiliation: Blood Bank, Santa María Clinic, Santiago de Chile, Chile.
authors.forename: S
authors.initials: S
authors.lastname: Mellado
authors.affiliation: Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Universidad de Talca, Talca, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Maldonado
authors.affiliation: Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Universidad de Talca, Talca, Chile. Electronic address: snunez@utalca.cl.
authors.forename: S
authors.initials: S
authors.lastname: Wehinger
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D015539:Platelet Activation; D017713:Platelet Transfusion; D010983:Plateletpheresis; D013997:Time Factors
keywords: Oxidative stress; Platelet; Platelet activation; Platelet apheresis; Storage lesion