title: Expression Levels of miR-30a-5p in Papillary Thyroid Carcinoma: A Comparison Between Serum and Fine Needle Aspiration Biopsy Samples.
abstract: BACKGROUND
  Fine needle aspiration biopsy (FNAB) is a useful tool in the diagnosis of thyroid nodules. However, some limitations exist as approximately 25% of the cases cannot be distinguished with this method. Therefore, identification of novel diagnostic markers is very important in improving the papillary thyroid carcinoma (PTC) diagnosis. microRNAs (miRNAs) are small regulatory RNA molecules that have been involved in a variety of biological processes, including tumorigenesis. Moreover, determination of miRNAs with prognostic, diagnostic, and therapeutic potential is of a great interest today.
  
  
  OBJECTIVE
  In the present study, we evaluated the expression level of miR-30a-5p in serum and FNAB samples of PTC patients.
  
  
  METHODS
  A total of 60 cases were included in the study, with the patients subdivided into four groups; benign, atypical cells of undetermined significance (ACUS), malignant group, including Hurthle cell PTC (HC-PTC), and malignant without Hurthle cell PTC (non-HC-PTC). Peripheral blood and FNAB samples of the cases were collected. The serum and FNAB expression levels of miR-30a-5p among the groups were compared. The miR-30a-5p expression level was determined using real-time polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  According to both pre- and postoperative pathological diagnosis, miR-30a-5p levels were significantly increased in both serum and FNAB samples of HC-PTC and non-HC-PTC groups compared to other groups. This increase was more evident in the non-HC-PTC group (p=0.0245 for FNAB, p=0.0166 for serum).
  
  
  CONCLUSIONS
  The results of this study suggest that miR-30a-5p might be a novel diagnostic marker candidate in PTC. Further studies are required to investigate this possibility.
authors.affiliation: 1 Department of Medical Biology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Yusuf Ziya
authors.initials: YZ
authors.lastname: Igci
authors.affiliation: 2 Department of Endocrinology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Mesut
authors.initials: M
authors.lastname: Ozkaya
authors.affiliation: 2 Department of Endocrinology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Hakan
authors.initials: H
authors.lastname: Korkmaz
authors.affiliation: 1 Department of Medical Biology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Esra
authors.initials: E
authors.lastname: Bozgeyik
authors.affiliation: 1 Department of Medical Biology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Recep
authors.initials: R
authors.lastname: Bayraktar
authors.affiliation: 1 Department of Medical Biology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ulasli
authors.affiliation: 3 Department of Pathology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Suna
authors.initials: S
authors.lastname: Erkilic
authors.affiliation: 2 Department of Endocrinology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Ayten
authors.initials: A
authors.lastname: Eraydin
authors.affiliation: 1 Department of Medical Biology, Faculty of Medicine, University of Gaziantep , Gaziantep, Turkey .
authors.forename: Serdar
authors.initials: S
authors.lastname: Oztuzcu
mesh_terms: D018249:Adenoma, Oxyphilic; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D044963:Biopsy, Fine-Needle / Q000379:methods; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D011379:Prognosis; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; C546086:MIRN30b microRNA, human; D035683:MicroRNAs