title: Effects of androstenedione exposure on fathead minnow (Pimephales promelas) reproduction and embryonic development.
abstract: High concentrations (300 ng/L) of androstenedione (A4) were identified in snowmelt runoff from fields fertilized with manure from livestock feeding operations in Wisconsin, USA. In fishes, A4 is an active androgen and substrate for biosynthesis of functional androgens (e.g., testosterone and 11-ketotestosterone) and estrogens (e.g., estradiol-17β). Thus, A4 has the potential to be a powerful endocrine disruptor. This hypothesis was tested by exposing reproductively mature fathead minnows to 0.0 ng/L, 4.5 ng/L, 74 ng/L, and 700 ng/L A4 for 26 d in a flow-through system. Various reproductive endpoints were measured including fecundity, fertilization success, secondary sexual characteristics, gonadosomatic index (GSI), and hepatic vitellogenin messenger RNA (mRNA) expression. In addition, fertilized embryos from the reproduction assay were used in an embryonic development assay to assess A4 effects on development and hatchability. In males, A4 significantly increased Vtg mRNA expression (estrogenic effect), significantly reduced GSI, and had no effect on tubercle expression (p = 0.067). In females, A4 induced tubercle development (androgenic effect) with no effects on GSI. Fecundity was not significantly impacted. Exposure to A4 had no effect on fertilization, embryonic development, or hatchability. These data indicate that exogenous A4, at environmentally relevant concentrations, can significantly modulate the reproductive physiology of the fathead minnows in a sex-specific manner and that A4 should be monitored as an endocrine disruptor.
authors.affiliation: Department of Zoology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Zachary A
authors.initials: ZA
authors.lastname: DeQuattro
authors.affiliation: Department of Animal Sciences, Laboratory of Fish Endocrinology and Aquaculture, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Jocelyn D C
authors.initials: JD
authors.lastname: Hemming
authors.affiliation: Department of Environmental Sciences, Wisconsin State Laboratory of Hygiene, Madison, Wisconsin, USA.
authors.forename: Terence P
authors.initials: TP
authors.lastname: Barry
mesh_terms: D000735:Androstenedione / Q000633:toxicity*; D000818:Animals; D003530:Cyprinidae / Q000502:physiology*; D047108:Embryonic Development / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000187:drug effects; D005306:Fertilization / Q000187:drug effects; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects*; D013739:Testosterone / Q000031:analogs & derivatives; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D012333:RNA, Messenger; D014819:Vitellogenins; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; C003600:11-ketotestosterone
keywords: Androstenedione; Concentrated animal feeding operations; Endocrine disruption; Reproductive toxicity; Risk assessment