title: Down-regulation of microRNA-26b modulates non-small cell lung cancer cells chemoresistance and migration through the association of PTEN.
abstract: To explore the effect of microRNA-26b (miR-26b) in non-small cell lung cancer (NSCLC) cells, we investigated the mRNA levels of miR-26b in 4 NSCLC cell lines and 10 clinical samples from human patients with NSCLC by quantitative reverse transcriptase polymerase chain reaction. It was found that miR-26b was significantly down-regulated in both NSCLC cells and human carcinoma tissues. Synthetic oligonucleotides were used to up-regulate or down-regulate miR-26b in NSCLC cell lines H1299 and A549 cells. Results showed that both down-regulating and up-regulating miR-26b had no effect on cancer cell proliferation in H1299 or A549 cells, whereas miR-26b over-expression increased cancer cell migration and reduced cisplatin chemosensitivity. Phosphatase and tensin homolog (PTEN) was confirmed to be directly bound by miR-26b by dual-luciferase reporter assay, and was down-regulated in miR-26b over-expressing NSCLC cells. Finally, when PTEN was up-regulated in NSCLC cells, it reversed the effects of miR-2b over-expression on NSCLC migration and cisplatin chemosensitivity. In conclusion, our data showed a functional mechanism of miR-26b in regulating NSCLC. It indicates that miR-26b may regulate NSCLC migration and chemosensitivity through the regulation of PTEN.
authors.affiliation: Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing 100730, China liannxin445@126.com.
authors.forename: Naixin
authors.initials: N
authors.lastname: Liang
authors.affiliation: Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, The General Hospital of the People's Liberation Army Hospital, Beijing 100853, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Dachun
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Shanqing
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Huaxia
authors.initials: H
authors.lastname: Yang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000502:physiology*; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C528163:MIRN26 microRNA, human; C494929:PTEN protein, human
keywords: NSCLC; PTEN; cisplatin; miR-26b; migration