title: The early days of blotting.
abstract: The history of the development of DNA blotting is described in this chapter. DNA blotting, involving the transfer of electrophoretically separated DNA fragments to a membrane support through capillary action, is also known as Southern blotting. This procedure enables the detection of a specific DNA sequence by hybridization with probes. The term Southern blotting led to a "geographic" naming tradition, with RNA blotting bearing the name Northern blotting and protein transfer to membranes becoming known as Western blotting.
authors.affiliation: Oxford Gene Technology, Begbroke Business Park, Sandy Lane, Yarnton, Oxford, OX5 1PF, UK, ed.southern@bioch.ox.ac.uk.
authors.forename: Edwin
authors.initials: E
authors.lastname: Southern
mesh_terms: D000818:Animals; D015139:Blotting, Southern / Q000266:history*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D049673:History, 20th Century; D049674:History, 21st Century; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics
chemical_list: D012313:RNA; D004247:DNA