title: Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy.
abstract: Babesia microti causes "emergency" human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells (Bm-RBCs) at the individual cell level. Through quantitative phase imaging techniques based on laser interferometry, we present the simultaneous measurements of structural, chemical, and mechanical modifications in individual mouse Bm-RBCs. 3-D refractive index maps of individual RBCs and in situ parasite vacuoles are imaged, from which total contents and concentration of dry mass are also precisely quantified. In addition, we examine the dynamic membrane fluctuation of Bm-RBCs, which provide information on cell membrane deformability.
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: HyunJoo
authors.initials: H
authors.lastname: Park
authors.affiliation: Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control &Prevention, Chungbuk 363-951, Republic of Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Kyoohyun
authors.initials: K
authors.lastname: Kim
authors.affiliation: Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control &Prevention, Chungbuk 363-951, Republic of Korea.
authors.forename: Shin-Hyeong
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control &Prevention, Chungbuk 363-951, Republic of Korea.
authors.forename: Won-Ja
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Youngchan
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Division of Malaria &Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control &Prevention, Chungbuk 363-951, Republic of Korea.
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: YongKeun
authors.initials: Y
authors.lastname: Park
mesh_terms: D000818:Animals; D041001:Babesia microti / Q000502:physiology*; D001404:Babesiosis / Q000097:blood / Q000469:parasitology*; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000469:parasitology* / Q000473:pathology*; D006696:Holography / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*