title: Effect of 830-nm laser phototherapy on olfactory neuronal ensheathing cells grown in vitro on novel bioscaffolds.
abstract: BACKGROUND
  The purpose of this study was to analyze olfactory ensheathing cell (OEC) proliferation and growth on Biosilicate and collagen bioscaffolds, and to determine whether the application of laser phototherapy would result in increased OEC proliferation on the scaffolds. The use of bioscaffolds is considered a promising strategy in a number of clinical applications where tissue healing is suboptimal. As in vitro OEC growth is a slow process, laser phototherapy could be useful to stimulate proliferation on bioscaffolds.
  
  
  METHODS
  OEC cells were seeded on the Biosilicate and collagen scaffolds. Seeded scaffolds were irradiated with a single exposure of 830-nm laser. Nonirradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed 7 days after irradiation.
  
  
  RESULTS
  OECs were successfully grown on discs composed of a glass-ceramic and collagen composite. Laser irradiation produced a 32.7% decrease and a 13.2% increase in OEC proliferation on glass-ceramic discs and on collagen scaffolds, respectively, compared with controls. Laser phototherapy resulted in a reduction in cell growth on the Biosilicate scaffolds and an increase in cell proliferation on collagen scaffolds.
  
  
  CONCLUSIONS
  These results were probably due to the nature of the materials. Future research combining laser phototherapy and glass-ceramic scaffolds should take into account possible interactions of the laser with matrix compounds.
authors.affiliation: Department of Bioscience, Federal University of São Paulo, Santos, SP - Brazil.
authors.forename: Ana Claudia Muniz
authors.initials: AC
authors.lastname: Renno
authors.affiliation: School of Biomolecular and Physical Sciences, Griffith University, Nathan, Queensland - Australia.
authors.forename: Pauline Ann
authors.initials: PA
authors.lastname: McDonnell
authors.affiliation: Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos, São Carlos, SP - Brazil.
authors.forename: Murilo
authors.initials: M
authors.lastname: Camuri Crovace
authors.affiliation: Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos, São Carlos, SP - Brazil.
authors.forename: Edgar Dutra
authors.initials: ED
authors.lastname: Zanotto
authors.affiliation: School of Physiotherapy and Exercise Science, Griffith University, Gold Coast, Queensland - Australia.
authors.forename: E-Liisa
authors.initials: EL
authors.lastname: Laakso
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects*; D003094:Collagen / Q000737:chemistry*; D028022:Low-Level Light Therapy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D018034:Olfactory Receptor Neurons / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D017640:Silicates / Q000737:chemistry; D013234:Stem Cells; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017640:Silicates; D003094:Collagen