title: Adhesion of Asaia bogorensis to Glass and Polystyrene in the Presence of Cranberry Juice.
abstract: The aim of the study was to evaluate the adhesion abilities of the acetic acid bacterium Asaia bogorensis to glass and polystyrene in the presence of American cranberry (Vaccinium macrocarpon) juice. The strain of A. bogorensis used was isolated from spoiled commercial fruit-flavored drinking water. The cranberry juice was analyzed for polyphenols, organic acids, and carbohydrates using high-performance liquid chromatography and liquid chromatography-mass spectrometry techniques. The adhesive abilities of bacterial cells in culture medium supplemented with cranberry juice were determined using luminometry and microscopy. The viability of adhered and planktonic bacterial cells was determined by the plate count method, and the relative adhesion coefficient was calculated. This strain of A. bogorensis was characterized by strong adhesion properties that were dependent upon the type of surface. The highest level of cell adhesion was found on the polystyrene. However, in the presence of 10% cranberry juice, attachment of bacterial cells was three times lower. Chemical analysis of juice revealed the presence of sugars, organic acids, and anthocyanins, which were identified as galactosides, glucosides, and arabinosides of cyanidin and peonidin. A-type proanthocyanidins responsible for the antiadhesion properties of V. macrocarpon also were detected.
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland. hubert.antolak@gmail.com.
authors.forename: Hubert
authors.initials: H
authors.lastname: Antolak
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kregiel
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Czyzowska
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000502:physiology*; D000872:Anthocyanins / Q000737:chemistry; D001422:Bacterial Adhesion; D002241:Carbohydrates; D002851:Chromatography, High Pressure Liquid; D018857:Food Packaging; D005638:Fruit / Q000737:chemistry; D000067030:Fruit and Vegetable Juices; D005898:Glass / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D013058:Mass Spectrometry; D011137:Polystyrenes / Q000737:chemistry*; D044945:Proanthocyanidins; D029799:Vaccinium macrocarpon / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D002241:Carbohydrates; D005960:Glucosides; D011137:Polystyrenes; D044945:Proanthocyanidins; D014867:Water; C017154:cyanidin; C473205:peonidin