title: Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots.
abstract: Cell encapsulation was developed to entrap viable cells within semi-permeable membranes. The engrafted encapsulated cells can exchange low molecular weight metabolites in tissues of the treated host to achieve long-term survival. The semipermeable membrane allows engrafted encapsulated cells to avoid rejection by the immune system. The encapsulation procedure was designed to enable a controlled release of bioactive compounds, such as insulin, other hormones, and cytokines. Here we describe a method for encapsulation of catabolic cells, which consume lipids for heat production and energy dissipation (thermogenesis) in the intra-abdominal adipose tissue of obese mice. Encapsulation of thermogenic catabolic cells may be potentially applicable to the prevention and treatment of obesity and type 2 diabetes. Another potential application of catabolic cells may include detoxification from alcohols or other toxic metabolites and environmental pollutants.
authors.affiliation: Department of Human Sciences, The Ohio State University.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Human Sciences, The Ohio State University.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Department of Minimally Invasive Surgery, The First Affiliated Hospital of Soochow University.
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Mao
authors.affiliation: NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University.
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
authors.affiliation: Department of Human Sciences, The Ohio State University; ziouzenkova.1@osu.edu.
authors.forename: Ouliana
authors.initials: O
authors.lastname: Ziouzenkova
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology*; D000464:Alginates; D000818:Animals; D018920:Coculture Techniques; D003584:Cytological Techniques / Q000379:methods*; D004339:Drug Compounding / Q000379:methods; D004734:Energy Metabolism; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D050356:Lipid Metabolism; D051379:Mice; D009765:Obesity; D011107:Polylysine
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011107:Polylysine; D020723:Glucuronic Acid