title: [Effect of electroacupuncture intervention combined with polysaccharide of Gastrodia elata Blume on expression of nestin and stem cell factor around the ischemic locus of frontal lobe cortex in local cerebral ischemia rats].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA), Polysaccharide of Gastrodia elata Blume (PGB), and EA + PGB on the expression of Nestin and stem cell factor (SCF) in the frontal lobe cortex around the ischemic loci of cerebral ischemia (CI) rats, so as to explore its mechanisms underlying improvement of CI.
  
  
  METHODS
  A total of 40 Sprague-Dawley adult rats were randomly divided into normal control, CI model, EA intervention, PGB intervention and EA + PGB groups (n = 8 in each group). The CI model was prepared by middle cerebral artery occlusion. EA (2 Hz, 2 V) was applied to "Baihui" (GV 20) and left "Zusanli" (ST 36) for 30 min, once daily for 14 days. Rats of the PGB and EA + PGB groups were treated by gastrogavage of PGB at a dose of 100 mg/kg, once daily for 14 successive days. The expression of Nestin and SCF in the frontal lobe around the ischemic loci of the frontal lobe was detected by immunohistochemistry.
  
  
  RESULTS
  Compared with the normal control group, the expression levels of regional cerebral cortical Nestin and SCF proteins were significantly increased in the model group (P < 0.05). After the treatment, the expression levels of Nestin and SCF were significantly further up-regulated in the EA, PGB and EA + PGB groups in comparison with the model group (P < 0.05).
  
  
  CONCLUSIONS
  EA combined with PGB can significantly up-regulate the expression of Nestin and SCF in the frontal lobe around the ischemic loci in cerebral ischemia rats, which may contribute to their function in improving CI.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Huai-Bin
authors.initials: HB
authors.lastname: Li
authors.forename: Ke-Ren
authors.initials: KR
authors.lastname: Xiong
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D003131:Combined Modality Therapy; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D015671:Electroacupuncture; D005625:Frontal Lobe / Q000378:metabolism*; D031668:Gastrodia / Q000737:chemistry*; D006801:Humans; D008297:Male; D064231:Nestin / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019089:Stem Cell Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D064231:Nestin; D019089:Stem Cell Factor