title: PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation.
abstract: Polycystic kidney disease-like (PKDL) genes that are expressed in sour taste cells have been proposed to be involved in the transduction of sourness by producing off-responses, which shows a large inward current after withdrawing the acid stimuli. However, the underlying mechanisms of off-responses are still unclear. Here, we demonstrate that an alkali-activated mechanism is responsible for eliciting off-responses, as evidenced by both experimental and theoretical analyses. In addition, we showed that the decaying phase of offset responses in PKD2L1/PKD1L3 channels was substantially accelerated by extracellular Ca(2+).
authors.affiliation: Key Laboratory for Biomedical Engineering of Ministry of Education of China, Department of Biomedical Engineering, School of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China.
authors.forename: Peihua
authors.initials: P
authors.lastname: Chen
authors.forename: Jin-zhi
authors.initials: JZ
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Jianhong
authors.initials: J
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Liu
mesh_terms: D000468:Alkalies / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000468:Alkalies; D015220:Calcium Channels; D007473:Ion Channels; C550142:PKD1L3 protein, human; C117185:PKD2L1 protein, human; D011956:Receptors, Cell Surface; D002118:Calcium