title: Perfluorononanoic acid disturbed the metabolism of lipid in the liver of streptozotocin-induced diabetic rats.
abstract: Most studies on the liver toxicity of perfluorinated compounds (PFCs) are focused on healthy individuals, whereas the effects of PFCs on individuals with diabetes mellitus have not been fully characterized. This study aimed to investigate the acute exposure of perfluorononanoic acid (PFNA) on the metabolism of lipid in the liver of streptozotocin-induced diabetic rats. Male diabetic rats were orally dosed by gavage for 7 days with 0, 0.2, 1 and 5 mg/kg/day PFNA. The contents of lipid, the activities of enzyme, the expressions of protein in the liver and the serum parameters were detected. The results indicate that dose-dependent accumulation of triglyceride and total cholesterol occurred in the livers of diabetic rats after PFNA treatment. PFNA increased the activities of lipid synthetase, fatty acid synthease, glucose-6-phosphate dehydrogenase and decreased the activity of lipolytic enzyme, hepatic lipase, in the liver of diabetic rats. The changes of the isocitrate dehydrogenase, malicenzyme and lipoprotein lipase were not obvious. The expressions of protein related to lipid homeostasis, liver X receptor α and apolipoprotein E, were decreased after PFNA administration. Exposure to PFNA also increased the activity of serum alanine aminotransferase in diabetic rats. In conclusion, this study discloses that exposure to PFNA impacts on enzymes and proteins related to liver lipid metabolism and lead to obvious accumulation of lipid in the liver of diabetic rats, which may be responsible for hepatotoxicity of this compound in individuals with diabetes mellitus.
authors.affiliation: a Department of Biological and Chemical Engineering , Suzhou University , Suzhou , P.R. China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Fang
authors.affiliation: a Department of Biological and Chemical Engineering , Suzhou University , Suzhou , P.R. China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Gao
authors.affiliation: a Department of Biological and Chemical Engineering , Suzhou University , Suzhou , P.R. China.
authors.forename: Xingtao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: a Department of Biological and Chemical Engineering , Suzhou University , Suzhou , P.R. China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D060467:Disease Resistance / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D005227:Fatty Acids; D005466:Fluorocarbons / Q000633:toxicity*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000139:chemically induced* / Q000150:complications / Q000378:metabolism / Q000503:physiopathology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D005227:Fatty Acids; D005466:Fluorocarbons; D014280:Triglycerides; C101816:perfluoro-n-nonanoic acid; D013311:Streptozocin; D002784:Cholesterol
keywords: Diabetes; hepatotoxicity; lipid metabolism; perfluorononanoic acid