title: Evaluation of photosynthetic electrons derivation by exogenous redox mediators.
abstract: Oxygenic photosynthesis is the complex process that occurs in plants or algae by which the energy from the sun is converted into an electrochemical potential that drives the assimilation of carbon dioxide and the synthesis of carbohydrates. Quinones belong to a family of species commonly found in key processes of the Living, like photosynthesis or respiration, in which they act as electron transporters. This makes this class of molecules a popular candidate for biofuel cell and bioenergy applications insofar as they can be used as cargo to ship electrons to an electrode immersed in the cellular suspension. Nevertheless, such electron carriers are mostly selected empirically. This is why we report on a method involving fluorescence measurements to estimate the ability of seven different quinones to accept photosynthetic electrons downstream of photosystem II, the first protein complex in the light-dependent reactions of oxygenic photosynthesis. To this aim we use a mutant of Chlamydomonas reinhardtii, a unicellular green alga, impaired in electron downstream of photosystem II and assess the ability of quinones to restore electron flow by fluorescence. In this work, we defined and extracted a "derivation parameter" D that indicates the derivation efficiency of the exogenous quinones investigated. D then allows electing 2,6-dichlorobenzoquinone, 2,5-dichlorobenzoquinone and p-phenylbenzoquinone as good candidates. More particularly, our investigations suggested that other key parameters like the partition of quinones between different cellular compartments and their propensity to saturate these various compartments should also be taken into account in the process of selecting exogenous quinones for the purpose of deriving photoelectrons from intact algae.
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Longatte
authors.affiliation: Laboratoire de physiologie membranaire et moléculaire du chloroplaste, CNRS, UPMC UMR 7141, I.B.P.C., 13 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Han-Yi
authors.initials: HY
authors.lastname: Fu
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Buriez
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Labbé
authors.affiliation: Laboratoire de physiologie membranaire et moléculaire du chloroplaste, CNRS, UPMC UMR 7141, I.B.P.C., 13 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Francis-André
authors.initials: FA
authors.lastname: Wollman
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Amatore
authors.affiliation: Laboratoire de physiologie membranaire et moléculaire du chloroplaste, CNRS, UPMC UMR 7141, I.B.P.C., 13 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Rappaport
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France. Electronic address: manon.guille@ens.fr.
authors.forename: Manon
authors.initials: M
authors.lastname: Guille-Collignon
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France. Electronic address: frederic.lemaitre@ens.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lemaître
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D004583:Electrons; D010788:Photosynthesis / Q000187:drug effects*; D011809:Quinones / Q000378:metabolism / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism
chemical_list: D011809:Quinones
keywords: Chlamydomonas reinhardtii algae; Fluorescence; Membranes; Photosynthesis; Photosystem II; Quinones