title: Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb/3T3 Embryonic Fibroblast Cell Line.
abstract: Metformin, a popular drug used to treat diabetes, has recently gained attention as a potentially useful therapeutic agent for treating cancer. In our research metformin was added to in vitro cultures of bone marrow-derived multipotent mesenchymal stromal cells (BMSCs) and Balb/3T3 fibroblast at concentration of 1 mM, 5 mM, and 10 mM. Obtained results indicated that metformin negatively affected proliferation activity of investigated cells. The drug triggered the formation of autophagosomes and apoptotic bodies in all tested cultures. Additionally, we focused on determination of expression of genes involved in insulin-like growth factor 2 (IGF2) signaling pathway. The most striking finding was that the mRNA level of IGF2 was constant in both BMSCs and Balb/3T3. Further, the analysis of IGF2 concentration in cell supernatants showed that it decreased in BMSC cultures after 5 and 10 mM metformin treatments. In case of Balb/3T3 the concentration of IGF2 in culture supernatants decreased after 1 and 5 mM and increased after 10 mM of metformin. Our results suggest that metformin influences the cytophysiology of somatic cells in a dose- and time-dependent manner causing inhibition of proliferation and abnormalities of their morphology and ultrastructure.
authors.affiliation: Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland ; Wrocławskie Centrum Badań EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland.
authors.forename: Agnieszka
authors.identifier: 0000-0002-7314-9821
authors.initials: A
authors.lastname: Śmieszek
authors.affiliation: Wrocławskie Centrum Badań EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Czyrek
authors.affiliation: Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Basinska
authors.affiliation: Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Trynda
authors.affiliation: Department of Biotechnology and Food Microbiology, Faculty of Food Science University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Skaradzińska
authors.affiliation: Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Siudzińska
authors.affiliation: Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland ; Wrocławskie Centrum Badań EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland.
authors.forename: Monika
authors.identifier: 0000-0002-7281-9272
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland ; Wrocławskie Centrum Badań EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D001854:Bone Marrow Cells / Q000187:drug effects / Q000648:ultrastructure*; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000648:ultrastructure*; D008687:Metformin / Q000008:administration & dosage*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C513121:IGF2 protein, mouse; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II; D008687:Metformin