title: Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κB signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets.
abstract: The severity of intestinal inflammation in mammals can be profoundly impacted by weaning stress. Cathelicidins protect intestinal homeostasis by not only directly killing bacteria but also immune regulators. Here, we investigated the effects of cathelicidin-BF (C-BF) derived from the snake venoms of Bungarus fasciatus on weaning stress and intestinal inflammation and examined the mechanisms by which C-BF modulates intestinal immune responses in weanling piglets. We found that C-BF treatment significantly increased performance and reduced the diarrheal index in weanling piglets. Serum IL-6, IL-22 and TNF-α production was decreased by C-BF treatment. We demonstrated that C-BF inhibited the expression of the inflammatory cytokines TNF-α, IL-6 and IL-8 but increased the expression of the anti-inflammatory cytokine IL-10 in the intestine. We also demonstrated that C-BF suppressed inflammation by down-regulating the nuclear factor-κB (NF-κB) signaling pathway in the intestine and in LPS-induced macrophages in vitro. However, C-BF significantly induced the phosphorylation of signal transducer and activator of transcription 1 (STAT-1) to enhance the phagocytosis of macrophages when inflammation was suppressed. In summary, our study demonstrated that C-BF suppressed intestinal inflammation by down-regulating the NF-κB signaling pathway and enhancing the phagocytosis of immune cells by activating STAT-1 during weaning.
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yi
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Caihua
authors.initials: C
authors.lastname: Yu
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Haiwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Deguang
authors.initials: D
authors.lastname: Song
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Denghu
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Du
authors.affiliation: Laboratory of Animal Nutrition and Feed Science, Department of Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China. Electronic address: yzwang321@zju.edu.cn.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D016207:Cytokines / Q000097:blood; D003967:Diarrhea / Q000473:pathology; D015536:Down-Regulation / Q000187:drug effects; D004751:Enteritis / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology*; D007422:Intestines / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D016328:NF-kappa B / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D050794:STAT1 Transcription Factor / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects*; D013315:Stress, Psychological / Q000150:complications; D034421:Sus scrofa; D013552:Swine; D014886:Weaning; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D050794:STAT1 Transcription Factor; D054804:Cathelicidins
keywords: Cathelicidin-BF; Inflammation; Nuclear factor-κB; Phagocytosis; STAT-1; Weaning stress