title: Morphological and molecular characterization of Nosema pernyi, a microsporidian parasite in Antheraea pernyi.
abstract: Nosema pernyi is a lethal pathogen that causes microsporidiosis in the Chinese oak silkworm, Antheraea pernyi. In this study, we presented its morphological and some molecular characteristics. The mature spores were measured to be 4.36 × 1.49 μm. The spore wall consisted of an electron-dense exospore (EX) and electron-lucent endospore (EN) layer. The polar filament (PF) was isofilar with 10-12 coils that were frequently arranged in a single row. Investigation results indicated that N. pernyi can infect the gut wall, silk glands, and other tissues. A full-length SMART cDNA library of N. pernyi was constructed, and then 824 expressed sequence tags (ESTs) were sequenced. Ninety unigenes, out of 197 assembled unigenes, showed significant homology to known genes of Nosema ceranae, Nosema bombycis, Encephalitozoon cuniculi, and other microsporidian species. Based on the nucleotide sequence of the α- and β-tubulin genes and amino acid sequence of actin gene, phylogenetic trees analysis showed that N. pernyi was closely related to Nosema philosamiae and Nosema antheraeae. It was correctly assigned to the Nosema group.
authors.affiliation: College of Bioscience and Biotechnology, Liaoning Engineering and Technology Resource Center for Insect Resource, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yiren
authors.initials: Y
authors.lastname: Jiang
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Muhammad
authors.initials: M
authors.lastname: Irfan
authors.forename: Shenglin
authors.initials: S
authors.lastname: Shi
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020224:Expressed Sequence Tags; D015723:Gene Library; D006790:Host-Parasite Interactions; D009036:Moths / Q000469:parasitology*; D016818:Nosema / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D010271:Parasites; D010802:Phylogeny; D013172:Spores, Fungal; D014404:Tubulin / Q000235:genetics
chemical_list: D014404:Tubulin