title: Recruitment and Immobilization of a Fluorinated Biomarker Across an Interfacial Phospholipid Film using a Fluorocarbon Gas.
abstract: Perfluorohexane gas when introduced in the air atmosphere above a film of phospholipid self-supported on an aqueous solution of C2F5-labeled compounds causes the recruitment and immobilization of the latter in the interfacial film. When the phospholipid forms a liquid-condensed Gibbs monolayer, which is the case for dipalmitoylphosphatidylcholine (DPPC), the C2F5-labeled molecule remains trapped in the monolayer after removal of F-hexane. Investigations involve bubble profile analysis tensiometry (Gibbs films), Langmuir monolayers and microbubble experiments. The new phenomenon was utilized to incorporate a hypoxia biomarker, a C2F5-labeled nitrosoimidazole (EF5), in microbubble shells. This finding opens perspectives in the delivery of fluorinated therapeutic molecules and biomarkers.
authors.affiliation: Institut Charles Sadron (CNRS), University of Strasbourg, 23 rue du Loess, 67034 Strasbourg (France).
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA (UK).
authors.forename: Miriam
authors.initials: M
authors.lastname: O'Duill
authors.affiliation: Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA (UK).
authors.forename: Véronique
authors.initials: V
authors.lastname: Gouverneur
authors.affiliation: Institut Charles Sadron (CNRS), University of Strasbourg, 23 rue du Loess, 67034 Strasbourg (France). krafft@unistra.fr.
authors.forename: Marie Pierre
authors.initials: MP
authors.lastname: Krafft
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism*; D016503:Drug Delivery Systems; D005466:Fluorocarbons / Q000737:chemistry*; D005740:Gases / Q000737:chemistry*; D006845:Hydrocarbons, Fluorinated / Q000008:administration & dosage*; D007093:Imidazoles / Q000008:administration & dosage*; D045423:Microbubbles; D009603:Nitroso Compounds / Q000008:administration & dosage*
chemical_list: D005466:Fluorocarbons; D005740:Gases; D006845:Hydrocarbons, Fluorinated; D007093:Imidazoles; D009603:Nitroso Compounds; D015060:1,2-Dipalmitoylphosphatidylcholine; C078626:perflexane
keywords: drug delivery; fluorocarbons; microbubbles; molecular recognition; phospholipid interfaces