title: [Nuclear protein 1 knockdown inhibits proliferation and migration of HepG2 cells].
abstract: OBJECTIVE
  To observe the expression of nuclear protein 1 (Nupr1), a stress-related nuclear protein, in a panel of human hepatocellular carcinoma cell lines, and its effects on the proliferation and migration of hepatocellular carcinoma cells by RNA interference-mediated knockdown.
  
  
  METHODS
  Real-time quantitative PCR was employed to detect Nupr1 mRNA levels in hepatocellular carcinoma cell lines, including BEL-7402, QSG-7703, SMMC-7721 and HepG2. After Nupr1 expression was knocked down by RNA interference, cell proliferation was monitored by MTT assay and colony formation in plate. Cell cycle was analyzed by flow cytometry and cell migration was assayed by TranswellTM assay.
  
  
  RESULTS
  Higher expression of Nupr1 was detected in HepG2 as compared with the other cell lines. Nupr1 expression in HepG2 cells were efficiently knocked down by two short hairpin RNAs (shRNAs), with inhibitory rates being 72.25% and 84.25%, respectively. HepG2 cells with Nupr1 knockdown displayed lower rate of proliferation, G1 arrest, and significantly decreased abilities of cell migration and colony formation. Western blotting showed that Nupr1 knockdown increased the expressions of p21 and p27, two negative regulators of cell cycle.
  
  
  CONCLUSIONS
  Knockdown of Nupr1 inhibited the proliferation and migration of HepG2 hepatocellular carcinoma cells.
authors.affiliation: Department of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Therapeutic Vaccine, Beijing 100038, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Therapeutic Vaccine, Beijing 100038, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Therapeutic Vaccine, Beijing 100038, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Therapeutic Vaccine, Beijing 100038, China.
authors.forename: Xinna
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Therapeutic Vaccine, Beijing 100038, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Manna
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Therapeutic Vaccine, Beijing 100038, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000172:deficiency* / Q000235:genetics*; D063646:Carcinogenesis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000172:deficiency* / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C491643:NUPR1 protein, human