title: Sealing ability of root-end filling materials.
abstract: The aim of this research was to compare the apical sealing ability of different root-end filling materials (SuperEBA(®), ProRoot MTA(®), thermoplasticized gutta-percha + AH-Plus(®), thermoplasticized RealSeal(®)), by means of microbial indicators.
  
  
  
  Thus, 50 human single-rooted teeth were employed, which were shaped until size 5 0, retro - prepared with ultrasonic tips and assigned to 4 groups, retro-filled with each material or controls. A platform was employed, which was split in two halves: upper chamber-where the microbial suspension containing the biological indicators was introduced (E. faecalis + S. aureus + P. aeruginosa + B. subtilis + C. albicans); and a lower chamber containing the culture medium brain, heart influsion, where 3 mm of the apical region of teeth were kept immersed. Lectures were made daily for 60 days, using the turbidity of the culture medium as indicative of microbial contamination. Statistical analyses were carried out at 5% level of significance.
  
  
  
  The results showed microbial leakage at least in some specimens in all of the groups. RealSeal(®) has more microbial leakage, statistically significant, compared to ProRoot(®) MTA and SuperEBA(®). No significant differences were observed when compared ProRoot(®) MTA and SuperEBA(®). The gutta-percha + AH Plus results showed no statistically significant differences when compared with the other groups.
  
  
  
  All the tested materials showed microbial leakage. Root-end fillings with Super-EBA or MTA had the lowest bacterial filtration and RealSeal shows highest bacterial filtration.
authors.affiliation: Department of Endodontics, University of Guadalajara, Jalisco, México.
authors.forename: Octávio
authors.initials: O
authors.lastname: Amezcua
authors.affiliation: Department of Endodontics, University of Guadalajara, Jalisco, México.
authors.forename: Álvaro Cruz
authors.initials: ÁC
authors.lastname: Gonzalez
authors.affiliation: Department of Endodontics, University of Cuiabá, MT, Brazil.
authors.forename: Álvaro Henrique
authors.initials: ÁH
authors.lastname: Borges
authors.affiliation: Department of Endodontics, University of Cuiabá, MT, Brazil.
authors.forename: Matheus Coelho
authors.initials: MC
authors.lastname: Bandeca
authors.affiliation: Department of Endodontics, University of Cuiabá, MT, Brazil.
authors.forename: Cyntia Rodrigues de Araújo
authors.initials: CR
authors.lastname: Estrela
authors.affiliation: Professor, Department of Stomatologic Sciences, Federal University of Goiás, Praça Universitária s/n, Setor Universitário CEP: 74605-220, Gaiânia, GO, Brazil, e-mail: estrela3@terra.com.br.
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry; D001412:Bacillus subtilis / Q000302:isolation & purification; D017610:Calcium Compounds / Q000737:chemistry; D002176:Candida albicans / Q000302:isolation & purification; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003763:Dental Leakage / Q000382:microbiology; D017220:Dentin-Bonding Agents / Q000737:chemistry; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004853:Epoxy Resins / Q000737:chemistry; D006171:Gutta-Percha / Q000737:chemistry; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D009391:Nephelometry and Turbidimetry / Q000379:methods; D010087:Oxides / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D011897:Random Allocation; D012184:Retrograde Obturation / Q000379:methods*; D012387:Root Canal Filling Materials / Q000737:chemistry*; D018915:Root Canal Preparation / Q000379:methods; D017640:Silicates / Q000737:chemistry; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D010087:Oxides; C511498:Real Seal composite resin; D012387:Root Canal Filling Materials; D017640:Silicates; C534916:epoxy resin-based root canal sealer; C086631:mineral trioxide aggregate; C084074:Super EBA; D006171:Gutta-Percha
keywords: Apical surgery; Microbial leakage; MTA; Resilon; Root-end filling materials; Super EBA