title: Inverse colloidal crystal membranes for hydrophobic interaction membrane chromatography.
abstract: Hydrophobic interaction membrane chromatography has gained interest due to its excellent performance in the purification of humanized monoclonal antibodies. The membrane material used in hydrophobic interaction membrane chromatography has typically been commercially available polyvinylidene fluoride. In this contribution, newly developed inverse colloidal crystal membranes that have uniform pores, high porosity and, therefore, high surface area for protein binding are used as hydrophobic interaction membrane chromatography membranes for humanized monoclonal antibody immunoglobulin G purification. The capacity of the inverse colloidal crystal membranes developed here is up to ten times greater than commercially available polyvinylidene fluoride membranes with a similar pore size. This work highlights the importance of developing uniform pore size high porosity membranes in order to maximize the capacity of hydrophobic interaction membrane chromatography.
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.|Ralph E Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Anh T
authors.initials: AT
authors.lastname: Vu
authors.affiliation: Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, USA.
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.|Ralph E Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, AR, USA.
authors.forename: S Ranil
authors.initials: SR
authors.lastname: Wickramasinghe
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Cong
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemical Engineering, Qingdao University, Qingdao, China.|Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, USA.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Tang
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D061067:Antibodies, Monoclonal, Humanized / Q000302:isolation & purification*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D016062:Porosity
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D008567:Membranes, Artificial
keywords: Membrane chromatography; Monoclonal antibodies; Polyvinylidene fluoride membrane; Protein purification; Uniform pore size