title: Collagenase as an effective tool for drug quantitation in tissues.
abstract: BACKGROUND
  In early drug-discovery research, traditional techniques to analyze drug concentrations in tissues for bioanalytical needs include bead beaters and probe homogenization devices, but are not as effective for tough fibrous tissues. To prepare these tissues, the enzyme collagenase was used to digest the collagen fibers present in epithelial and connective tissue.
  
  
  RESULTS
  The benefits of tissue homogenization using a bead beater following collagenase treatment of samples, as opposed to using bead beating alone, was investigated. Matrix effect, recovery factor and stability with and without collagenase were assessed.
  
  
  CONCLUSIONS
  Little to no effects on the quality and reliability of collagenase treated samples were observed. This enzymatic approach is a feasible and effective tool for tissue homogenization and subsequent analysis by LC-MS/MS.
authors.forename: Ann Ran-Ran
authors.initials: AR
authors.lastname: Qin
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liang
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Deng
authors.forename: Brian
authors.initials: B
authors.lastname: Dean
authors.forename: Sheerin K
authors.initials: SK
authors.lastname: Shahidi-Latham
mesh_terms: D000818:Animals; D017364:Collagenases / Q000378:metabolism*; D055808:Drug Discovery; D005240:Feasibility Studies; D005615:Freezing; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats
chemical_list: D004364:Pharmaceutical Preparations; D017364:Collagenases