title: First isolation and establishment of in vitro culture of Theileria lestoquardi from a naturally infected cow.
abstract: Theileria infected cell line was isolated from the prescapular lymph node of an adult crossbred cow. Molecular study confirmed this cell line of bovine lymphocyte has been transformed by the Theileria lestoquardi. This strain of T. lestoquardi designated Ka-6 and sheep were inoculated with this strain didn't show any clinical signs of theileriosis which shows the significance of this cell line to develop a tissue-culture vaccine against malignant ovine theileriosis. Contrary to accepted belief that the T. lestoquardi not capable of causing disease in cattle, the present study describes the first isolation and establishment of in vitro culture of T. lestoquardi-infected cell line from a naturally infected cow with typical singes of acute theileriosis.
authors.affiliation: Razi Vaccine and Serum Research Institute, Shiraz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Namavari
authors.affiliation: Islamic Azad University of Jahrom, Jahrom, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Ezhdehakosh-Pour
authors.affiliation: Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: G R
authors.initials: GR
authors.lastname: Habibi
authors.affiliation: Razi Vaccine and Serum Research Institute, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Rahimian
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Namazi
keywords: Cell line; Cow; Theileria lestoquardi