title: Identification of a Circulating MicroRNA Profile as a Biomarker of Metastatic Cutaneous Melanoma.
abstract: No specific biomarkers for prognostication or evaluation of tumour load in melanoma have been reported to our knowledge. MicroRNAs (miRNAs) are strongly implicated in oncogenesis and tumour progression, and their circulating forms have been studied as potential biomarkers in oncology. The aim of this prospective study was to identify a melanoma-specific profile of plasma miRNAs. A screening phase, using RNA microarray, was conducted on plasma from 14 patients with metastatic melanoma and 5 healthy subjects. Selected miRNAs were analysed by RTqPCR in 2 independent training and validation cohorts including, respectively, 29 and 31 patients and 16 and 43 control subjects. A profile of 2 miRNAs (miR-1246 and miR-185) significantly associated with metastatic melanoma with a sensitivity of 90.5% and a specificity of 89.1% was identified. This plasma miRNA profile may become an accurate non-invasive biomarker for melanoma.
authors.affiliation: Inserm, UMR 1037-CRCT, FR-31052 Toulouse, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Armand-Labit
authors.forename: Nicolas
authors.initials: N
authors.lastname: Meyer
authors.forename: Anne
authors.initials: A
authors.lastname: Casanova
authors.forename: Henri
authors.initials: H
authors.lastname: Bonnabau
authors.forename: Valérie
authors.initials: V
authors.lastname: Platzer
authors.forename: Emilie
authors.initials: E
authors.lastname: Tournier
authors.forename: Benoît
authors.initials: B
authors.lastname: Sansas
authors.forename: Stéphane
authors.initials: S
authors.lastname: Verdun
authors.forename: Benoit
authors.initials: B
authors.lastname: Thouvenot
authors.forename: Benoit
authors.initials: B
authors.lastname: Hilselberger
authors.forename: Andrei
authors.initials: A
authors.lastname: Doncescu
authors.forename: Laurence
authors.initials: L
authors.lastname: Lamant
authors.forename: Magali
authors.initials: M
authors.lastname: Lacroix-Triki
authors.forename: Gilles
authors.initials: G
authors.lastname: Favre
authors.forename: Anne
authors.initials: A
authors.lastname: Pradines
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008545:Melanoma / Q000097:blood* / Q000235:genetics / Q000556:secondary; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C577700:MIRN1246 microRNA, human; C552431:MIRN185 microRNA, human; D035683:MicroRNAs