title: CD4+CD29+T cells are blamed for the persistent inflammatory response in ulcerative colitis.
abstract: Ulcerative colitis (UC) is a chronic gastrointestinal disorder eliciting occurrence of colorectal cancer, the third most common human malignancy. The diagnosis of UC is based on clinical symptoms combined with typical findings on endoscopy, radiology, and ultimately pathology. We investigated the variation trend of CD4+CD29+T cells together with MPO, VCAM-1 in different periods of rat UC model and UC patients. We also evaluated the relationship between CD4+CD29+T cells and disease severity. UC model was induced by administering DNCB liquid and acetate solution. We found upregulated expression of CD4+CD29+T cells in both peripheral blood and colon from rats, and a similar trend for MPO and VCAM-1 in colon (P<0.05); the expression was especially enhanced in UC rats at two weeks after the model was established (P<0.01). Such upregulation was also indicated in active and remission UC patients as compared to the healthy and enteritis groups (P<0.05), with the highest expression level detected in the active UC patients (P<0.01). Pearson correlation analysis showed a positive correlation of CD4+CD29+T cells in rat and human peripheral blood with DAI score (rrat=0.712, rhuman=0.677, P<0.01), and MPO in colon (rrat=0.514, rhuman=0.682, P<0.05). These results suggest that CD4+CD29+T cells may act as major effector cell subsets in persistent inflammatory responses for UC and that infiltration into colon inflammation may be induced by the combination of VCAM-1 and CD29.
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical College Zhanjiang City, Guangdong, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Digestive System, Huai'an First People's Hospital, Nanjing Medical University Huai'an City, Jiangsu, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Spleen and Stomach Diseases, Tai'an Hospital of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine Tai'an City, Shandong, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical College Zhanjiang City, Guangdong, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical College Zhanjiang City, Guangdong, China ; Department of Pharmacology, Guangdong Medical College Zhanjiang City, Guangdong, China.
authors.forename: Cancan
authors.initials: C
authors.lastname: Guo
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical College Zhanjiang City, Guangdong, China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University Guangzhou City, Guangdong, China.
authors.forename: Shixue
authors.initials: S
authors.lastname: Dai
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical College Zhanjiang City, Guangdong, China.
authors.forename: Xuebao
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003093:Colitis, Ulcerative / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D019012:Integrin beta1 / Q000276:immunology; D008297:Male; D009195:Peroxidase / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
chemical_list: D019012:Integrin beta1; D019010:Vascular Cell Adhesion Molecule-1; D009195:Peroxidase
keywords: CD4+CD29+T cells; Ulcerative colitis; VCAM-1; persistent inflammatory responses