title: Local administration of stromal cell-derived factor-1 promotes stem cell recruitment and bone regeneration in a rat periodontal bone defect model.
abstract: Stromal cell-derived factor-1 (SDF-1) recruits adult stem/progenitor cells via its specific receptor, C-X-C motif receptor 4 (CXCR4), to promote heart, kidney and tendon regeneration, but little is known about the effects of SDF-1 on bone regeneration in periodontal diseases. The objective of this study was to investigate whether local administration of SDF-1 in a collagen membrane scaffold enhanced the recruitment of host stem cells and improved periodontal bone defect repair. To this end, bone defects were established on the buccal side of bilateral mandibles in Wistar rats. After application of collagen membranes loaded with SDF-1 or phosphate-buffered saline (PBS) to the defects, the effects of SDF-1 on stem cell recruitment, inflammatory cell responses, angiogenesis, osteoclastogenesis, scaffold degradation, and bone regeneration were evaluated. It showed that SDF-1 recruited host-derived mesenchymal stem cells and hematopoietic stem cells to the wound area and significantly reduced the CD11b+ inflammatory cell response. Moreover, SDF-1 increased vascular formation, induced early bone osteoclastogenesis, accelerated scaffold degradation, and promoted the quality and quantity of regenerated bone. Our results suggest that this cell-free approach by local administration of SDF-1 may be an effective strategy for development as a simple and safe technique for periodontal bone regeneration.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China; The Second Hospital of Shandong University, Department of Stomatology, Jinan, Shandong Province, China.
authors.forename: Lingqian
authors.initials: L
authors.lastname: Du
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. Electronic address: yangps@sdu.edu.cn.
authors.forename: Pishan
authors.initials: P
authors.lastname: Yang
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. Electronic address: shaohuage@sdu.edu.cn.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D054377:Chemokine CXCL12 / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002633:Chemotaxis / Q000187:drug effects*; D003094:Collagen / Q000627:therapeutic use; D008338:Mandibular Injuries / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D054377:Chemokine CXCL12; D003094:Collagen
keywords: Chemotaxis; Mesenchymal stem cells; Periodontal bone regeneration; SDF-1; Tissue engineering