title: Vitamin D Promotes Odontogenic Differentiation of Human Dental Pulp Cells via ERK Activation.
abstract: The active metabolite of vitamin D such as 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) is a well-known key regulatory factor in bone metabolism. However, little is known about the potential of vitamin D as an odontogenic inducer in human dental pulp cells (HDPCs) in vitro. The purpose of this study was to evaluate the effect of vitamin D3 metabolite, 1α,25(OH)2D3, on odontoblastic differentiation in HDPCs. HDPCs extracted from maxillary supernumerary incisors and third molars were directly cultured with 1α,25(OH)2D3 in the absence of differentiation-inducing factors. Treatment of HDPCs with 1α,25(OH)2D3 at a concentration of 10 nM or 100 nM significantly upregulated the expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein1 (DMP1), the odontogenesis-related genes. Also, 1α,25(OH)2D3 enhanced the alkaline phosphatase (ALP) activity and mineralization in HDPCs. In addition, 1α,25(OH)2D3 induced activation of extracellular signal-regulated kinases (ERKs), whereas the ERK inhibitor U0126 ameliorated the upregulation of DSPP and DMP1 and reduced the mineralization enhanced by 1α,25(OH)2D3. These results demonstrated that 1α,25(OH)2D3 promoted odontoblastic differentiation of HDPCs via modulating ERK activation.
authors.affiliation: Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.|Medical Research Center for Biomineralization Disorders, Chonnam National University, Gwangju 500-757, Korea.|Department of Oral Physiology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Woo
authors.affiliation: Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.|Department of Dental Education, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Hae-Soon
authors.initials: HS
authors.lastname: Lim
authors.affiliation: Department of Dental Hygiene, Honam University, Gwangju 506-714, Korea.
authors.forename: Kyung-Yi
authors.initials: KY
authors.lastname: Jeong
authors.affiliation: Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.|Department of Pediatric Dentistry, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Seon-Mi
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.|Medical Research Center for Biomineralization Disorders, Chonnam National University, Gwangju 500-757, Korea.|Department of Oral Physiology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.|Department of Oral Physiology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Jung
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D008963:Molar / Q000166:cytology; D009805:Odontogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D014807:Vitamin D; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C118756:dihydroxy-vitamin D3
keywords: 1α,25(OH)2D3; human dental pulp cells; mitogen-activated protein kinase; odontoblastic differentiation; vitamin D