title: Effects of skin-derived precursors on wound healing of denervated skin in a nude mouse model.
abstract: Denervated skin could result in impaired healing of wounds, such as decubitus ulcers and diabetic foot ulcers. Other studies indicated that cutaneous fiber density is reduced after inner nerve transection and that neuropeptide level depletes after denervation, leading to reduced cell proliferation around the wound and thus wound healing problems. Recent studies have revealed that skin-derived precursors (SKPs), which form a neural crest-related stem cell population in the dermis of skin, participate in cutaneous nerve regeneration. We hypothesized that injecting SKPs into denervated wound promotes healing. A bilateral denervation wound model was established followed by SKP transplantation. The wound healing rate was determined at 7, 14, and 21 d after injury. Cell proliferation activity during wound healing was analyzed by proliferating cell nuclear antigen immunohistochemistry (IHC). Nerve fiber density was measured by S-100 IHC. The contents of nerve growth factor, substance P, and calcitonin gene-related peptide were examined by enzyme-linked immunosorbent assay. The rate of epithelization in the SKP-treated group was faster than that in the control group. Wound cell proliferation and nerve fiber density were obviously higher in the SKP-treated group than in the control group. In addition, the content of neuropeptides was higher in the SKP-treated group than in the control group during wound healing. In conclusion, SKPs can promote denervated wound healing through cell proliferation and nerve fiber regeneration, and can facilitate the release of neuropeptides.
authors.affiliation: Department of Burns, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.affiliation: Department of Burns, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Ju-Lin
authors.initials: JL
authors.lastname: Xie
authors.affiliation: Department of Burns, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Xu
authors.affiliation: Department of Burns, Guangdong General Hospital Guangzhou, Guangdong, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Lai
authors.affiliation: Department of Emergency, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Dermatology, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Mao
authors.affiliation: Department of Burns, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Xu-Sheng
authors.initials: XS
authors.lastname: Liu
authors.affiliation: Department of Burns, First Affiliated Hospital of Sun Yat-Sen University Guangzhou, Guangdong, China.
authors.forename: Shao-Hai
authors.initials: SH
authors.lastname: Qi
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D049109:Cell Proliferation; D003714:Denervation; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009416:Nerve Regeneration / Q000502:physiology*; D058953:Neural Stem Cells / Q000637:transplantation*; D012867:Skin / Q000294:innervation*; D033581:Stem Cell Transplantation / Q000379:methods*; D013373:Substance P / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D013373:Substance P; D015740:Calcitonin Gene-Related Peptide
keywords: SKP; cell transplantation; denervation; neuropeptides; wound healing