title: Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification.
abstract: This assay, termed branched cascade enzymatic amplification (BCEA), can be a novel and straightforward method for sensitive and specific microRNA detection in crude cellular extracts of cancer cells at physiological temperature, by coupling two ordinary polymerases, Klenow fragment exo(-) and terminal deoxynucleotidyl transferase.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, People's Republic of China. jdqiu@ncu.edu.cn.
authors.forename: Bao-Zhu
authors.initials: BZ
authors.lastname: Chi
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Liang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian-Ding
authors.initials: JD
authors.lastname: Qiu
mesh_terms: D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000032:analysis* / Q000096:biosynthesis*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D035683:MicroRNAs; D004253:DNA Nucleotidylexotransferase; D004256:DNA Polymerase I