title: Authentication of "Cereza del Jerte" sweet cherry varieties by free zone capillary electrophoresis (FZCE).
abstract: The purpose of this work was to develop a procedure based on protein analysis by free zone capillary electrophoresis (FZCE) that can be used as an alternative to other methods in the determination of sweet cherry varieties for the authentication of "Cereza del Jerte". Two autochthonous varieties of sweet cherry type "Picota", 'Ambrunés' and 'Pico Negro', and the foreign variety 'Sweetheart' were used in the study. Two protocols for extracting the methanol-soluble proteins were tested. On the basis of the results, direct evaporation with nitrogen of a methanol extract was included in the extraction protocol for routine analysis. This method was found to give excellent repeatability of the corrected migration time (CMT), and showed greater effectiveness in discriminating sweet cherry varieties than the SDS-PAGE technique. Three peaks found in the FZCE electropherograms were investigated as a basis for discriminating between varieties. In addition, the FZCE analysis of methanol-soluble proteins provides information about the physico-chemical parameters relevant to the sensorial quality of the sweet cherries.
authors.affiliation: Hortofruticultura, Centro de Investigación Finca La Orden-Valdesequera, Junta de Extremadura, Autovía Madrid-Lisboa s/n, Guadajira, 06187 Badajoz, Spain.
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Serradilla
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain. Electronic address: amartin@unex.es.
authors.forename: Alberto
authors.initials: A
authors.lastname: Martín
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Aranda
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Hernández
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Benito
authors.affiliation: Hortofruticultura, Centro de Investigación Finca La Orden-Valdesequera, Junta de Extremadura, Autovía Madrid-Lisboa s/n, Guadajira, 06187 Badajoz, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Lopez-Corrales
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura,(1) Ctra. de Cáceres s/n, 06071 Badajoz, Spain.
authors.forename: María de Guía
authors.initials: Mde G
authors.lastname: Córdoba
keywords: Authentication; FZCE; Protein profile; Sweet cherry