title: Gene Editing in One-Cell Embryos by Zinc-Finger and TAL Nucleases.
abstract: Gene targeting by sequence-specific nucleases in one-cell embryos provides an expedited mutagenesis approach in rodents. This technology has been recently established to create knockout and knockin mutants through sequence deletion or sequence insertion. This article provides protocols for the preparation and microinjection of nuclease mRNA and targeting vector DNA into fertilized mouse eggs. Furthermore, we provide guidelines for genotyping the desired mouse mutants. Curr. Protoc. Mouse Biol. 2:347-364 © 2012 by John Wiley & Sons, Inc.
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Wefers
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.|Chair for Developmental Genetics, Technische Universität München, Munich, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Meyer
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.|Chair for Developmental Genetics, Technische Universität München, Munich, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Hensler
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.|Chair for Developmental Genetics, Technische Universität München, Munich, Germany.
authors.forename: Sudeepta
authors.initials: S
authors.lastname: Panda
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.
authors.forename: Oskar
authors.initials: O
authors.lastname: Ortiz
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.|Chair for Developmental Genetics, Technische Universität München, Munich, Germany.|Max Planck Institute of Psychiatry, Molecular Neurogenetics, Munich, Germany.|Deutsches Zentrum für Neurodegenerative Erkrankungen, Standort München, Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.affiliation: Helmholtz Center Munich, Institute for Developmental Genetics Munich, Germany.|Chair for Developmental Genetics, Technische Universität München, Munich, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Kühn
keywords: TAL nuclease; gene targeting; homologous recombination; mouse mutant; pronucleus injection; zinc-finger nuclease