title: Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level.
abstract: DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, 8000 Aarhus C, Denmark. kjems@inano.au.dk.
authors.forename: A H
authors.initials: AH
authors.lastname: Okholm
authors.forename: H
authors.initials: H
authors.lastname: Aslan
authors.forename: F
authors.initials: F
authors.lastname: Besenbacher
authors.forename: M
authors.initials: M
authors.lastname: Dong
authors.forename: J
authors.initials: J
authors.lastname: Kjems
mesh_terms: D000418:Albumins; D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D058105:Polymerization; D013499:Surface Properties
chemical_list: D000418:Albumins; D004247:DNA; D004253:DNA Nucleotidylexotransferase