title: Arbuscular mycorrhiza affects nickel translocation and expression of ABC transporter and metallothionein genes in Festuca arundinacea.
abstract: Mycorrhizal fungi are key microorganisms for enhancing phytoremediation of soils contaminated with heavy metals. In this study, the effects of the arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae (=Glomus mosseae) on physiological and molecular mechanisms involved in the nickel (Ni) tolerance of tall fescue (Festuca arundinacea = Schedonorus arundinaceus) were investigated. Nickel addition had a pronounced negative effect on tall fescue growth and photosynthetic pigment contents, as well as on AMF colonization. Phosphorus content increased markedly in mycorrhizal plants (M) compared to non-inoculated (NM) ones. However, no significant difference was observed in root carbohydrate content between AMF-inoculated and non-inoculated plants. For both M and NM plants, Ni concentrations in shoots and roots increased according to the addition of the metal into soil, but inoculation with F. mosseae led to significantly lower Ni translocation from roots to the aboveground parts compared to non-inoculated plants. ABC transporter and metallothionein transcripts accumulated to considerably higher levels in tall fescue plants colonized by F. mosseae than in the corresponding non-mycorrhizal plants. These results highlight the importance of mycorrhizal colonization in alleviating Ni-induced stress by reducing Ni transport from roots to shoots of tall fescue plants.
authors.affiliation: Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran. lshabani@gmail.com.
authors.forename: Leila
authors.initials: L
authors.lastname: Shabani
authors.affiliation: Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Sabzalian
authors.affiliation: Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran.
authors.forename: Sodabeh
authors.initials: S
authors.lastname: Mostafavi pour
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D031751:Festuca / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D055137:Glomeromycota / Q000378:metabolism; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008903:Minerals / Q000378:metabolism; D038821:Mycorrhizae / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009532:Nickel / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018520:Plant Shoots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D018528:ATP-Binding Cassette Transporters; D008903:Minerals; D012987:Soil; D012989:Soil Pollutants; D002734:Chlorophyll; D002338:Carotenoids; D009532:Nickel; D008668:Metallothionein
keywords: ABC transporters; Metallothionein; Mycorrhiza; Nickel; Schedonorus arundinaceus; Tall fescue