title: Association analysis of -416 G>C polymorphism of T-cell immunoglobulin and mucin domain-1 gene with asthma in Iran.
abstract: TIM (T-cell immunoglobulin (Ig) and mucin domain)-1, one of the members of TIM family, expresses on Th2 cells and promotes the production of Th2 signature cytokines. This can increase a series of responses in these cells which could be one of the causes of asthma or asthma-related phenotypes. The aim of this study was to investigate whether a TIM-1 promoter single nucleotide polymorphism (SNP), -416 G>C, is associated with asthma in Iranian population. In this case-control study, existence of the -416 G>C polymorphism was assessed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in 300 patients with asthma (97 atopic, 203 nonatopic) and 309 healthy volunteers. Additionally, the relationship between these polymorphism genotypes and total serum IgE levels in this Iranian population was evaluated. We discovered a significant association between the -416 G>C polymorphism and atopic asthma susceptibility in the population, but this SNP showed no connection with nonatopic asthma (P < 0.05). However, our results showed significant relation between this polymorphism and serum IgE level (P < 0.05). Our results suggest that -416 G>C polymorphism in TIM-1 gene could be a predisposing factor for atopic asthma in Iranian population, and CC genotype of this SNP can be associated with increased level of IgE in patients with asthma in the same population.
authors.affiliation: Department of Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Shirzade
authors.affiliation: Department of Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Meshkat
authors.affiliation: Department of Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.|Cellular & Molecular Immunology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.|Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ganjalikhani-Hakemi
authors.affiliation: Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Mosayebian
authors.affiliation: Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Ghasemi
authors.affiliation: Department of Statistics, School of health, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Deress
authors.affiliation: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Parchami Barjui
authors.affiliation: Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Sadri
authors.affiliation: Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Salehi
mesh_terms: D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000473:pathology; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D000072596:Hepatitis A Virus Cellular Receptor 1; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics; D007492:Iran; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011991:Receptors, Virus / Q000235:genetics*
chemical_list: C491143:HAVCR1 protein, human; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D007073:Immunoglobulin E