title: L-Phenylalanine catabolism and 2-phenylethanol synthesis in Yarrowia lipolytica--mapping molecular identities through whole-proteome quantitative mass spectrometry analysis.
abstract: A world-wide effort is now being pursued towards the development of flavors and fragrances (F&F) production independently from traditional sources, as well as autonomously from depleting fossil fuel supplies. Biotechnological production of F&F by microbes has emerged as a vivid solution to the current market limitations. Amongst a wide variety of fragrant chemicals, 2-PE is of significant interest to both scientific and industrial community. Although the general overview of the 2-PE synthesis pathway is commonly known, involvement of particular molecular identities in this pathway has not been elucidated in Yarrowia lipolytica to date. The aim of this study was mapping molecular identities involved in 2-PE synthesis in Y. lipolytica. To acquire a comprehensive landscape of the proteins that are directly and indirectly involved in L-Phe degradation and 2-PE synthesis, we took advantage of comprehensibility and sensitivity of high-throughput LC-MS/MS-quantitative analysis. Amongst a number of proteins involved in amino acid turnover and the central carbon metabolism, enzymes involved in L-Phe conversion to 2-PE have been identified. Results on yeast-to-hyphae transition in relation to the character of the provided nitrogen source have been presented.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland celinska@up.poznan.pl.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Celińska
authors.affiliation: Department of Biochemistry, Medical University of Gdansk, 80-210 Gdańsk, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Olkowicz
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Grajek
mesh_terms: D015536:Down-Regulation / Q000235:genetics; D005421:Flavoring Agents / Q000737:chemistry; D005579:Fossil Fuels; D020869:Gene Expression Profiling; D057166:High-Throughput Screening Assays; D010649:Phenylalanine / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation / Q000235:genetics; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
chemical_list: D005421:Flavoring Agents; D005579:Fossil Fuels; D020543:Proteome; D010649:Phenylalanine; D010626:Phenylethyl Alcohol
keywords: 2-phenylethanol; L-phenylalanine; Yarrowia lipolytica, proteomics; amino acid metabolism; aroma compounds