title: Arabidopsis AINTEGUMENTA mediates salt tolerance by trans-repressing SCABP8.
abstract: The Arabidopsis AINTEGUMENTA (ANT) gene, which encodes an APETALA2 (AP2)-like transcription factor, controls plant organ cell number and organ size throughout shoot development. ANT is thus a key factor in the development of plant shoots. Here, we have found that ANT plays an essential role in conferring salt tolerance in Arabidopsis. ant-knockout mutants presented a salt-tolerant phenotype, whereas transgenic plants expressing ANT under the 35S promoter (35S:ANT) exhibited more sensitive phenotypes under high salt stress. Further analysis indicated that ANT functions mainly in the shoot response to salt toxicity. Target gene analysis revealed that ANT bound to the promoter of SOS3-LIKE CALCIUM BINDING PROTEIN 8 (SCABP8), which encodes a putative Ca(2+) sensor, thereby inhibiting expression of SCABP8 (also known as CBL10). It has been reported that the salt sensitivity of scabp8 is more prominent in shoot tissues. Genetic experiments indicated that the mutation of SCABP8 suppresses the ant-knockout salt-tolerant phenotype, implying that ANT functions as a negative transcriptional regulator of SCABP8 upon salt stress. Taken together, the above results reveal that ANT is a novel regulator of salt stress and that ANT binds to the SCABP8 promoter, mediating salt tolerance.
authors.affiliation: School of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui 741001, People's Republic of China Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, People's Republic of China Division of Molecular Life Sciences, Pohang University of Science and Technology, Hyoja-dong, Pohang, Kyungbuk 790-784, Republic of Korea menglaisheng@mail.kib.ac.cn ybwang2015@yeah.net liuaizhong@mail.kib.ac.cn.
authors.forename: Lai-Sheng
authors.initials: LS
authors.lastname: Meng
authors.affiliation: School of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui 741001, People's Republic of China menglaisheng@mail.kib.ac.cn ybwang2015@yeah.net liuaizhong@mail.kib.ac.cn.
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Wang
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, People's Republic of China.
authors.forename: Shun-Qiao
authors.initials: SQ
authors.lastname: Yao
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, People's Republic of China menglaisheng@mail.kib.ac.cn ybwang2015@yeah.net liuaizhong@mail.kib.ac.cn.
authors.forename: Aizhong
authors.initials: A
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics* / Q000502:physiology; D055051:Salt-Tolerant Plants / Q000235:genetics*; D012965:Sodium Chloride; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C099055:AINTEGUMENTA protein, Arabidopsis; D029681:Arabidopsis Proteins; C484078:CBL10 protein, Arabidopsis; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; C477653:SOS1 protein, Arabidopsis; C112941:SOS3 protein, Arabidopsis; D017923:Sodium-Hydrogen Exchangers; D014157:Transcription Factors; D012965:Sodium Chloride; C406738:SOS2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: AINTEGUMENTA (ANT); Arabidopsis; CALCINEURIN B-LIKE10; SCABP8; SOS3-LIKE CALCIUM BINDING PROTEIN8; Salt stress; Shoot; Trans-repression