title: The role of Duffy antigen receptor for chemokines in keloids.
abstract: This study aims to determine the relationship between Duffy antigen receptor for chemokines (DARC) and keloid pathogenesis. DARC expression was determined by immunohistochemistry, real-time PCR, and Western blot analysis. Cell proliferation was assessed by CCK-8 assay. Cell migration and invasion abilities were measured by the shift assay. Levels of CC chemokine ligand 2 (CCL2), CXC chemokine ligand 8 (CXCL8), and matrix metalloproteinase 2 (MMP2) were detected by real-time PCR and ELISA. Our results showed that DARC levels were elevated in human keloid fibroblasts. After knocking down DARC, cell proliferation was not altered, whereas the migration and invasion abilities of keloid fibroblasts were significantly elevated. Additionally, the mRNA expression levels of CCL2, CXCL8, and MMP2 were not influenced by DARC knockdown. However, the secretion of CCL2, but not CXCL8 or MMP2, was significantly increased after DARC knockdown. Our results suggest that DARC might inhibit the secretion of CCL2. Moreover, DARC knockdown increases the migration and invasion abilities of keloid fibroblasts.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China; Department of Breast Surgery, Guangdong Traditional Chinese Medicine Hospital, Guangzhou 510000, Guangdong, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China; Department of Plastic Surgery, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong, China.
authors.forename: Nong
authors.initials: N
authors.lastname: Liao
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Pathology, Guangdong Traditional Chinese Medicine Hospital, Guangzhou 510000, Guangdong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China. Electronic address: 15989280535@139.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Gao
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D004375:Duffy Blood-Group System / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: C473745:ACKR1 protein, human; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D004375:Duffy Blood-Group System; D011956:Receptors, Cell Surface
keywords: Chemokine; Duffy antigen receptor for chemokines (DARC); Keloids; Migration; Proliferation