title: Cationic electrophoresis and eastern blotting.
abstract: Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis, staining, and blotting of such gels are presented.
authors.affiliation: Ross University School of Medicine, 266, Roseau, Dominica, West Indies, engelbert_buxbaum@web.de.
authors.forename: Engelbert
authors.initials: E
authors.lastname: Buxbaum
mesh_terms: D001391:Azo Compounds / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015151:Immunoblotting / Q000379:methods*
chemical_list: D001391:Azo Compounds; D002593:Cetrimonium Compounds; C032756:ponceau S; D000077286:Cetrimonium