title: Immunohistochemical and genetic markers to distinguish hemangiopericytoma and meningioma.
abstract: Hemangiopericytoma (HPC) and meningioma, for their morphology immunohistochemical markers similarity, were usually confused especially before surgery. This study aimed to develop a panel of biomarkers to differentiate HPC from meningioma. Real-time PCR and immunohistochemical staining were employed to determine the levels of p53, bcl-2, c-myc, vimentin, CD34, FVIIIa, MGMT and reticular fiber in 15 meningiomas, HPCs and their normal controls. We found that, in the mRNA expression level, both Bcl-2 and c-myc were high in HPC and meningiomas, but bcl-2 was higher in HPC than in meningiomas, c-myc was lower in HPC than in meningiomas. In protein expression level, reticular fibers were around most HPC tumor cells but observed null in meningiomas; CD34 and FVIIIa were both found positive in HPCs but negative in meningiomas; MGMT was weak focal in HPC but strong diffuse in meningiomas. In conclusion, bcl-2, c-myc, and MGMT could be employed as the new panels of biomarkers for distinguishing HPC from meningiomas.
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Nanjing Brain Hospital Nanjing 210029, Jiangsu, China.
authors.forename: Penglai
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Fudan University Huashan Hospital Shanghai 200040, China.
authors.forename: Tongming
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Fudan University Huashan Hospital Shanghai 200040, China.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Nanjing Brain Hospital Nanjing 210029, Jiangsu, China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Fudan University Huashan Hospital Shanghai 200040, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Nanjing Brain Hospital Nanjing 210029, Jiangsu, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhang
keywords: Meningioma; hemangiopericytoma; immunohistochemistry