title: Transcriptional repression by MYB3R proteins regulates plant organ growth.
abstract: In multicellular organisms, temporal and spatial regulation of cell proliferation is central for generating organs with defined sizes and morphologies. For establishing and maintaining the post-mitotic quiescent state during cell differentiation, it is important to repress genes with mitotic functions. We found that three of the Arabidopsis MYB3R transcription factors synergistically maintain G2/M-specific genes repressed in post-mitotic cells and restrict the time window of mitotic gene expression in proliferating cells. The combined mutants of the three repressor-type MYB3R genes displayed long roots, enlarged leaves, embryos, and seeds. Genome-wide chromatin immunoprecipitation revealed that MYB3R3 binds to the promoters of G2/M-specific genes and to E2F target genes. MYB3R3 associates with the repressor-type E2F, E2FC, and the RETINOBLASTOMA RELATED proteins. In contrast, the activator MYB3R4 was in complex with E2FB in proliferating cells. With mass spectrometry and pairwise interaction assays, we identified some of the other conserved components of the multiprotein complexes, known as DREAM/dREAM in human and flies. In plants, these repressor complexes are important for periodic expression during cell cycle and to establish a post-mitotic quiescent state determining organ size.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan JST, CREST, Chikusa, Nagoya, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Iwata
authors.affiliation: WPI Institute of Transformative Bio-Molecules, Nagoya University, Chikusa, Nagoya, Japan Graduate School of Sciences, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Nakamichi
authors.affiliation: Graduate School of Sciences, Nagoya University, Chikusa, Nagoya, Japan JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
authors.forename: Poyu
authors.initials: P
authors.lastname: Chen
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Ohtani
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishida
authors.affiliation: Department of Biology, Tokyo Gakugei University, Koganei, Tokyo, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Hosoya
authors.affiliation: Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Müller
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Szeged, Hungary.
authors.forename: Tünde
authors.initials: T
authors.lastname: Leviczky
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Szeged, Hungary.
authors.forename: Aladár
authors.initials: A
authors.lastname: Pettkó-Szandtner
authors.affiliation: Laboratory of Proteomic Research, Biological Research Centre, Szeged, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Darula
authors.affiliation: Department of Biology, Tokyo Gakugei University, Koganei, Tokyo, Japan.
authors.forename: Akitoshi
authors.initials: A
authors.lastname: Iwamoto
authors.affiliation: Graduate School of Sciences, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Nomoto
authors.affiliation: Center for Gene Research, Division of Biological Science, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Tada
authors.affiliation: WPI Institute of Transformative Bio-Molecules, Nagoya University, Chikusa, Nagoya, Japan Graduate School of Sciences, Nagoya University, Chikusa, Nagoya, Japan JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Chikusa, Nagoya, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higashiyama
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Demura
authors.affiliation: The National Plant Phenomics Centre, Aberystwyth University, Aberystwyth, UK.
authors.forename: John H
authors.initials: JH
authors.lastname: Doonan
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Marie-Theres
authors.initials: MT
authors.lastname: Hauser
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan JST, CREST, Ikoma, Nara, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Umeda
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Royal Holloway, School of Biological Sciences, University of London, Egham, Surrey, UK.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Magyar
authors.affiliation: Royal Holloway, School of Biological Sciences, University of London, Egham, Surrey, UK.
authors.forename: László
authors.initials: L
authors.lastname: Bögre
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan JST, CREST, Chikusa, Nagoya, Japan masakito@agr.nagoya-u.ac.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ito
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D013058:Mass Spectrometry; D046228:Microarray Analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism; D038081:Organogenesis / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D046912:Multiprotein Complexes; D012097:Repressor Proteins; D014157:Transcription Factors
keywords: DREAM complex; G2/M phase; MYB transcription factors; cell cycle regulation; cell differentiation