title: Morphologic characteristics of the placental basal plate in in vitro fertilization pregnancies: a possible association with the amount of bleeding in delivery.
abstract: The aim of the present study was to investigate the relationship between assisted reproductive technology procedures, the morphology of the basal plate of placentas, and amount of bleeding in deliveries. Fifty-five whole placentas (fresh-embryo transfer in the in vitro fertilization cycle [n = 6], frozen-thawed embryo transfer in the natural cycle [n = 13] or in the hormonal cycle [n = 10], and age-matched spontaneously conceived pregnancies [n = 26]) were retrospectively enrolled and histologically analyzed. The whole placentas were stored in our pathological division among 512 singleton pregnancies with vaginal deliveries (34-41 weeks of gestation) at Hamamatsu University Hospital. The morphology of the placental basal plate was examined using Azan staining. A total of 20 digital images (each 0.53 mm(2)) of microscopic fields were analyzed per placenta to measure the mean values of the vertical maximum thickness of Rohr and Nitabuch fibrinoid layers and % loss of decidua. The thickness of Rohr fibrinoid layer and % loss of decidua were significantly higher in the frozen-thawed embryo transfer in the hormonal cycle group than in the frozen-thawed embryo transfer in the natural cycle and spontaneously conceived pregnancy groups (each P < .01). The z scores for both the thickness of Rohr fibrinoid layer and % loss of decidua positively correlated with those for the amount of bleeding in deliveries (P < .05 each). Assisted reproductive technology procedures changed the morphology of the placental basal plate, suggesting a possible association with an increase in the amount of bleeding in deliveries.
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Yaguchi
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan. Electronic address: hitou-endo@umin.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Itoh
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Sakamoto
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Furuta
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Uchida
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Tamura
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Kazunao
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Tsuruga Nursing University, Tsuruga 914-0814, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sumimoto
authors.affiliation: ACT Tower Clinic, Hamamatsu 430-7707, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Matsuda
authors.affiliation: ACT Tower Clinic, Hamamatsu 430-7707, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Nishimura Women's Clinic, Hamamatsu 433-8122, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nishimura
authors.affiliation: Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kanayama
mesh_terms: D036861:Delivery, Obstetric / Q000009:adverse effects*; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects*; D006470:Hemorrhage / Q000209:etiology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000473:pathology*; D011247:Pregnancy
keywords: Assisted reproductive technology (ART); Delivery; Placenta; Postpartum hemorrhage (PPH); Pregnancy