title: The hepatoprotective role of Silymarin in isoniazid induced liver damage of rabbits.
abstract: OBJECTIVE
  To evaluate the hepatoprotective role of Silymarin against isonicotinylhydrazine-induced hepatotoxicity in rabbit model.
  
  
  METHODS
  The experimental animal study was held at Jinnah Postgraduate Medical Centre, Karachi, from April to September 2013 and comprised rabbits weighing 1-1.5kgof either gender. The animals were divided randomly into equal groups: group I underwent liver function test without any drug; in group II effects of Silymarin (50mg/kg/day orally) was observed; in group III isoniazid (50mg/kg/dayorally) was administered; and in group IV combined effects of isoniazid and silymarin were observed. Liver function tests were performed at day0 and after the treatment at day19. SPSS 16 was used for statistical analysis.
  
  
  RESULTS
  The 28 rabbits in the study were divided in four groups of 7(25%) each. No mortality was recorded in any group. In group III, bilirubin level was increased and alanine transaminase was decreased significantly (p<0.05 each). In group IV, there was significant improvement in serum billirubin and serum alanine transaminase (p<0.05 each).
  
  
  CONCLUSIONS
  Isonicotinylhydrazine-induced hepatotoxicity was well treated by concurrent administration of Silymarin.
authors.affiliation: Department of Pharmacology and Therapeutics, BMSI, Jinnah Postgraduate Medical Center (JPMC), Karachi.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Jahan
authors.affiliation: Department of Pharmacology and Therapeutics, BMSI, Jinnah Postgraduate Medical Center (JPMC), Karachi.
authors.forename: Moosa
authors.initials: M
authors.lastname: Khan
authors.affiliation: Department of Pharmacology and Therapeutics, BMSI, Jinnah Postgraduate Medical Center (JPMC), Karachi.
authors.forename: Sana
authors.initials: S
authors.lastname: Imran
authors.affiliation: Department of Pharmacology and Therapeutics, BMSI, Jinnah Postgraduate Medical Center (JPMC), Karachi.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sair
mesh_terms: D000410:Alanine Transaminase / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000995:Antitubercular Agents / Q000633:toxicity*; D001663:Bilirubin / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D005260:Female; D007538:Isoniazid / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008111:Liver Function Tests; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D012838:Silymarin / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D020011:Protective Agents; D012838:Silymarin; D000410:Alanine Transaminase; D001663:Bilirubin; D007538:Isoniazid
keywords: Hepatotoxicity, INH-induced hepatotoxicity, Silymarin, Liver function test.