title: Clonal analysis of human dendritic cell progenitor using a stromal cell culture.
abstract: Different dendritic cell (DC) subsets co-exist in humans and coordinate the immune response. Having a short life, DCs must be constantly replenished from their progenitors in the bone marrow through hematopoiesis. Identification of a DC-restricted progenitor in mouse has improved our understanding of how DC lineage diverges from myeloid and lymphoid lineages. However, identification of the DC-restricted progenitor in humans has not been possible because a system that simultaneously nurtures differentiation of human DCs, myeloid and lymphoid cells, is lacking. Here we report a cytokine and stromal cell culture that allows evaluation of CD34(+) progenitor potential to all three DC subsets as well as other myeloid and lymphoid cells, at a single cell level. Using this system, we show that human granulocyte-macrophage progenitors are heterogeneous and contain restricted progenitors to DCs.
authors.affiliation: Columbia University Medical Center, Department of Microbiology and Immunology, New York, NY 10032, USA.
authors.forename: Jaeyop
authors.initials: J
authors.lastname: Lee
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Breton
authors.affiliation: Columbia University Medical Center, Department of Microbiology and Immunology, New York, NY 10032, USA.
authors.forename: Arafat
authors.initials: A
authors.lastname: Aljoufi
authors.affiliation: Columbia University Medical Center, Department of Microbiology and Immunology, New York, NY 10032, USA.
authors.forename: Yu Jerry
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: Columbia University Medical Center, Department of Microbiology and Immunology, New York, NY 10032, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Puhr
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
authors.affiliation: Columbia University Medical Center, Department of Microbiology and Immunology, New York, NY 10032, USA. Electronic address: kl2529@columbia.edu.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000276:immunology; D006410:Hematopoiesis / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D013234:Stem Cells / Q000276:immunology*; D017154:Stromal Cells / Q000276:immunology*
chemical_list: D018952:Antigens, CD34
keywords: Culture; Dendritic cell; Granulocyte–macrophage progenitor; Hematopoiesis; Single cell; Stromal cells