title: Photoscopic characterization of green synthesized silver nanoparticles from Trichosanthes tricuspidata and its antibacterial potential.
abstract: The present study focused on the finding of reducing agents for the formation of silver nanoparticles (AgNPs) from the plant, Trichosanthes tricuspidata. The synthesized AgNPs were characterized using UV-Visible spectroscopy, particle size analyzer (PSA), X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM) analyses. The UV-Visible spectrum resulted a sharp peak (at 430nm) represents the strong plasmon resonance of silver. The average size distributions of AgNPs were found to be 78.49nm, through (PSA), and the silver ion with its crystalline nature was confirmed using intensity (2θ) peak value of 38.22°, 44.66°, 64.61°, and 77.49°. The SEM micrograph revealed that the synthesized AgNPs have a spherical morphology with the size ranges from 20 to 28nm. AFM showed the presence of polydispersed AgNPs with its size (20 to 60nm in height). The gas chromatography-mass spectroscopy (GC-MS) study analyzed the responsible compounds present in the methanolic extracts for the bio-reduction of AgNPs and their antibacterial effect was studied. AgNPs exhibited preponderant activity than the methanolic extracts on clinical pathogens. Thus, the synthesized AgNPs might act as an effective antibacterial agent. Further studies are required to isolate the specific compound responsible for the reduction capability and its their inhibitory mechanisms for target bacterial strains.
authors.affiliation: Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem 636011, Tamil Nadu, India.
authors.forename: Ragunathan
authors.initials: R
authors.lastname: Yuvarajan
authors.affiliation: Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem 636011, Tamil Nadu, India. Electronic address: natarajpu@gmail.com.
authors.forename: Devarajan
authors.initials: D
authors.lastname: Natarajan
authors.affiliation: Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem 636011, Tamil Nadu, India.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Ragavendran
authors.affiliation: Centre for Nanoscience and Technology, Anna University, Chennai 600025, Tamil Nadu, India.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Jayavel
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles; D000432:Methanol / Q000737:chemistry; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D019163:Reducing Agents / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D029778:Trichosanthes / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D019163:Reducing Agents; D012834:Silver; D000432:Methanol
keywords: Antibacterial activity; Silver nanoparticles; Spectral studies; T. tricuspidata