title: Differential changes in luteinizing hormone secretion after administration of the investigational metastin/kisspeptin analog TAK-683 in goats.
abstract: This study aimed to evaluate the hormonal and ovarian responses to the administration of a metastin/kisspeptin analog (TAK-683) under the endocrine environments of luteal and follicular phases in goats. Five estrous cycling goats received a prostaglandin F2α injection followed by 10 days of progesterone treatment by CIDR. The TAK-683 (35nmol) was intravenously administered (Hour 0) on 3 days after CIDR insertion (luteal phase condition; LC) and at 12h after CIDR removal (follicular phase condition; FC). Blood samples were collected at 10min (-2 to 6h), 2h (6-24h) or 6h intervals (24-48h). In the LC, small increases in the basal concentrations of LH were observed after TAK-683 administration from 0 to 6h, which were associated with an increase in estradiol concentration, followed by a surge-like release of LH with a peak at 12.5±1.0h (n=4) after TAK-683 administration. In the FC, a surge-like release of LH occurred immediately after TAK-683 administration with a peak at 6.0±3.5h (n=5), which was earlier than that in the LC (P<0.01). The peak concentration of estradiol did not differ between the two conditions, whereas the time interval from TAK-683 treatment to estradiol peak in the LC was longer than that in the FC (12.0±0.0 compared with 6.0±4.2h, P<0.05). These findings suggest that the timing of surge-like release of LH after TAK-683 administration is associated with blood estradiol concentration at the time of treatment.
authors.affiliation: Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Endo
authors.affiliation: Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Tamesaki
authors.affiliation: Nagoya University, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohkura
authors.affiliation: National Institute of Agrobiological Sciences, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wakabayashi
authors.affiliation: Takeda Pharmaceutical Company, Ltd., Japan.
authors.forename: Hisanori
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Takeda Pharmaceutical Company, Ltd., Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Takeda Pharmaceutical Company, Ltd., Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: National Institute of Agrobiological Sciences, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamura
authors.affiliation: Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology, Japan. Electronic address: tomomit@cc.tuat.ac.jp.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000187:drug effects / Q000502:physiology; D005260:Female; D006041:Goats / Q000502:physiology; D007275:Injections, Intravenous / Q000662:veterinary; D059648:Kisspeptins / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D010053:Ovary / Q000000981:diagnostic imaging / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D014463:Ultrasonography
chemical_list: D059648:Kisspeptins; C578072:TAK-683; D004958:Estradiol; D007986:Luteinizing Hormone
keywords: Goat; Kisspeptin; Luteinizing hormone; Ovarian follicle; Ovulation