title: Single-cell genome and transcriptome processing prior to high-throughput sequencing.
abstract: Single-cell genome and transcriptome characterizations will probe to be decisive within the stem cells research, especially to describe appropriately the genetic impact of the diverse stem cells populations that are present in each organism. In the present chapter, we describe in detail how to prepare sequencing libraries out of single cells, for whole genome DNA and mRNA sequencing.
authors.affiliation: Genome Analysis Platform, CIC bioGUNE & CIBERehd, Technological Park of Bizkaia - Building 801A, 48160, Derio, Spain, amaransay@cicbiogune.es.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Aransay
authors.forename: Laura
authors.initials: L
authors.lastname: Barcena
authors.forename: Aintzane
authors.initials: A
authors.lastname: Gonzalez-Lahera
authors.forename: Nuria
authors.initials: N
authors.lastname: Macias-Camara
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods; D015723:Gene Library; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D059010:Single-Cell Analysis / Q000379:methods; D059467:Transcriptome