title: Intravenously delivered neural stem cells migrate into ischemic brain, differentiate and improve functional recovery after transient ischemic stroke in adult rats.
abstract: Stem cell transplantation may provide an alternative therapy to promote functional recovery after various neurological disorders including cerebral infarct. Due to the minimal immunogenicity and neuronal differentiation potential of neural stem cells (NSCs), we tested whether intravenous administration of mice-derived C17.2 NSCs could improve neurological function deficit and cerebral infarction volume after ischemic stroke in rats. Additionally, we evaluated the survival, migration, proliferation, and differentiation capacity of transplanted NSCs in the rat brain. Intravenous infusion of NSCs after middle cerebral artery occlusion (MCAO) showed better performance in neurobiological severity scores after MCAO compared to control. However, the volume of cerebral infarction was not different at 7 days after MCAO compared with control. Transplanted NSCs were detected in the ischemic region but not in the contralateral hemisphere. NSCs differentiated into neurons or astrocytes after MCAO. These data suggest that intravenously transplanted NSCs can migrate, proliferate, and differentiate into neurons and astrocytes in the rat brain with focal ischemia and improve functional recovery.
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China ; Present address: Department of Pharmacy, Longyou People's Hospital Quzhou, Zhejiang, 324400, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China.
authors.forename: Jinjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China ; Translation Medicine Research Center, Lishui People's Hospital, Wenzhou Medical University Lishui, Zhejiang 323000, China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Bioengineering and The McGowan Institute for Regenerative Medicine, University of Pittsburgh Pittsburgh, PA 15219, USA.
authors.forename: Noah R
authors.initials: NR
authors.lastname: Johnson
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China.
authors.forename: Xichong
authors.initials: X
authors.lastname: Yu
authors.affiliation: Translation Medicine Research Center, Lishui People's Hospital, Wenzhou Medical University Lishui, Zhejiang 323000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Translation Medicine Research Center, Lishui People's Hospital, Wenzhou Medical University Lishui, Zhejiang 323000, China.
authors.forename: Tianzheng
authors.initials: T
authors.lastname: Lou
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Cixi People's Hospital, Wenzhou Medical University Ningbo 315300, PR China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Neurosurgery, Cixi People's Hospital, Wenzhou Medical University Ningbo 315300, PR China.
authors.forename: Zaifeng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurology, Zhoushan Hospital, Wenzhou Medical University Zhoushan, Zhejiang 316000, PR China.
authors.forename: Songbin
authors.initials: S
authors.lastname: He
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D064593:Heterografts; D002546:Ischemic Attack, Transient / Q000601:surgery*; D008297:Male; D051379:Mice; D058953:Neural Stem Cells / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D033581:Stem Cell Transplantation / Q000379:methods*
keywords: Ischemic stroke; cell transplantation; intravenous administration; neural stem cells