title: Transcriptional landscape of trans-kingdom communication between Candida albicans and Streptococcus gordonii.
abstract: Recent studies have shown that the transcriptional landscape of the pleiomorphic fungus Candida albicans is highly dependent upon growth conditions. Here using a dual RNA-seq approach we identified 299 C. albicans and 72 Streptococcus gordonii genes that were either upregulated or downregulated specifically as a result of co-culturing these human oral cavity microorganisms. Seventy-five C. albicans genes involved in responses to chemical stimuli, regulation, homeostasis, protein modification and cell cycle were significantly (P ≤ 0.05) upregulated, whereas 36 genes mainly involved in transport and translation were downregulated. Upregulation of filamentation-associated TEC1 and FGR42 genes, and of ALS1 adhesin gene, concurred with previous evidence that the C. albicans yeast to hypha transition is promoted by S. gordonii. Increased expression of genes required for arginine biosynthesis in C. albicans was potentially indicative of a novel oxidative stress response. The transcriptional response of S. gordonii to C. albicans was less dramatic, with only eight S. gordonii genes significantly (P ≤ 0.05) upregulated at least two-fold (glpK, rplO, celB, rplN, rplB, rpsE, ciaR and gat). The expression patterns suggest that signals from S. gordonii cause a positive filamentation response in C. albicans, whereas S. gordonii appears to be transcriptionally less influenced by C. albicans.
authors.affiliation: School of Oral and Dental Sciences, University of Bristol, Bristol, UK.
authors.forename: L C
authors.initials: LC
authors.lastname: Dutton
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: K H
authors.initials: KH
authors.lastname: Paszkiewicz
authors.affiliation: School of Oral and Dental Sciences, University of Bristol, Bristol, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Silverman
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: P R
authors.initials: PR
authors.lastname: Splatt
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: S
authors.initials: S
authors.lastname: Shaw
authors.affiliation: School of Oral and Dental Sciences, University of Bristol, Bristol, UK.
authors.forename: A H
authors.initials: AH
authors.lastname: Nobbs
authors.affiliation: University of Louisville School of Dentistry, Louisville, KY, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Lamont
authors.affiliation: School of Oral and Dental Sciences, University of Bristol, Bristol, UK.
authors.forename: H F
authors.initials: HF
authors.lastname: Jenkinson
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: M
authors.initials: M
authors.lastname: Ramsdale
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion / Q000235:genetics; D018441:Biofilms; D002176:Candida albicans / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015966:Gene Expression Regulation, Fungal; D056265:Microbial Interactions; D009055:Mouth / Q000382:microbiology*; D054773:Streptococcus gordonii / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: C093538:ALS1 protein, Candida albicans; D018829:Adhesins, Bacterial; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C489432:TEC1 protein, Candida albicans; D014157:Transcription Factors
keywords: GPI anchor; RNA-Seq; adhesin; bacteria-fungi interactions; cell wall proteins