title: Biological Properties and Characterization of ASL50 Protein from Aged Allium sativum Bulbs.
abstract: Allium sativum is well known for its medicinal properties. The A. sativum lectin 50 (ASL50, 50 kDa) was isolated from aged A. sativum bulbs and purified by gel filtration chromatography on Sephacryl S-200 column. Agar well diffusion assay were used to evaluate the antimicrobial activity of ASL50 against Candida species and bacteria then minimal inhibitory concentration (MIC) was determined. The lipid A binding to ASL50 was determined by surface plasmon resonance (SPR) technology with varying concentrations. Electron microscopic studies were done to see the mode of action of ASL50 on microbes. It exerted antimicrobial activity against clinical Candida isolates with a MIC of 10-40 μg/ml and clinical Pseudomonas aeruginosa isolates with a MIC of 10-80 μg/ml. The electron microscopic study illustrates that it disrupts the cell membrane of the bacteria and cell wall of fungi. It exhibited antiproliferative activity on oral carcinoma KB cells with an IC50 of 36 μg/ml after treatment for 48 h and induces the apoptosis of cancer cells by inducing 2.5-fold higher caspase enzyme activity than untreated cells. However, it has no cytotoxic effects towards HEK 293 cells as well as human erythrocytes even at higher concentration of ASL50. Biological properties of ASL50 may have its therapeutic significance in aiding infection and cancer treatments.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Kumar
authors.forename: Kumar
authors.initials: K
authors.lastname: Jitendra
authors.forename: Kusum
authors.initials: K
authors.lastname: Singh
authors.forename: Vaishali
authors.initials: V
authors.lastname: Kapoor
authors.forename: Mou
authors.initials: M
authors.lastname: Sinha
authors.forename: Immaculata
authors.initials: I
authors.lastname: Xess
authors.forename: Satya N
authors.initials: SN
authors.lastname: Das
authors.forename: Sujata
authors.initials: S
authors.lastname: Sharma
authors.forename: Tej P
authors.initials: TP
authors.lastname: Singh
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Dey
mesh_terms: D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005737:Garlic / Q000737:chemistry*; D057809:HEK293 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007624:KB Cells; D008050:Lipid A / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*; D017421:Sequence Analysis
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D008050:Lipid A; D037121:Plant Lectins