title: Enhanced chrysene degradation by a mixed culture Biorem-CGBD using response surface design.
abstract: Degradation of chrysene, a four ringed highly carcinogenic polycyclic aromatic hydrocarbon (PAH) has been demonstrated by bacterial mixed culture Biorem-CGBD comprising Achromobacter xylosoxidans, Pseudomonas sp. and Sphingomonas sp., isolated from crude oil polluted saline sites at Bhavnagar coast, Gujarat, India. A full factorial Central Composite Design (CCD) using Response Surface Methodology (RSM) was applied to construct response surfaces, predicting 41.93% of maximum chrysene degradation with an experimental validation of 66.45% chrysene degradation on 15th day, using a combination of 0.175, 0.175 and 0.385 mL of OD600 = 1 inoculum of A. xylosoxidans, Pseudomonas sp. and Sphingomonas sp., respectively and a regression coefficient (R2) of 0.9485 indicating reproducibility of the experiment. It was observed that chrysene degradation can be successfully enhanced using RSM, making mixed culture Biorem-CGBD a potential bioremediation target for PAH contaminated saline sites.
authors.forename: Bharti P
authors.initials: BP
authors.lastname: Dave
authors.forename: Chirag M
authors.initials: CM
authors.lastname: Ghevariya
authors.forename: Jwalant K
authors.initials: JK
authors.lastname: Bhatt
authors.forename: Dushyant R
authors.initials: DR
authors.lastname: Dudhagara
authors.forename: Rahul K
authors.initials: RK
authors.lastname: Rajpara
mesh_terms: D042441:Achromobacter denitrificans / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D002273:Carcinogens / Q000737:chemistry / Q000378:metabolism; D002911:Chrysenes / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry* / Q000633:toxicity; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism; D020578:Sphingomonas / Q000737:chemistry / Q000378:metabolism
chemical_list: D002273:Carcinogens; D002911:Chrysenes; D011084:Polycyclic Aromatic Hydrocarbons; C031180:chrysene