title: Extreme multifunctional proteins identified from a human protein interaction network.
abstract: Moonlighting proteins are a subclass of multifunctional proteins whose functions are unrelated. Although they may play important roles in cells, there has been no large-scale method to identify them, nor any effort to characterize them as a group. Here, we propose the first method for the identification of 'extreme multifunctional' proteins from an interactome as a first step to characterize moonlighting proteins. By combining network topological information with protein annotations, we identify 430 extreme multifunctional proteins (3% of the human interactome). We show that the candidates form a distinct sub-group of proteins, characterized by specific features, which form a signature of extreme multifunctionality. Overall, extreme multifunctional proteins are enriched in linear motifs and less intrinsically disordered than network hubs. We also provide MoonDB, a database containing information on all the candidates identified in the analysis and a set of manually curated human moonlighting proteins.
authors.affiliation: 1] Aix-Marseille University, TAGC, Marseille F-13009, France [2] INSERM UMR_S1090, Marseille F-13009, France.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Chapple
authors.affiliation: 1] Aix-Marseille University, TAGC, Marseille F-13009, France [2] INSERM UMR_S1090, Marseille F-13009, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Robisson
authors.affiliation: 1] Aix-Marseille University, TAGC, Marseille F-13009, France [2] INSERM UMR_S1090, Marseille F-13009, France [3] Aix-Marseille University, CIML, Marseille F-13009, France [4] CNRS, UMR 7280, Marseille F-13009, France [5] INSERM, U631, Marseille F-13009, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Spinelli
authors.affiliation: 1] Aix-Marseille University, TAGC, Marseille F-13009, France [2] INSERM UMR_S1090, Marseille F-13009, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Guien
authors.affiliation: 1] Aix-Marseille University, TAGC, Marseille F-13009, France [2] INSERM UMR_S1090, Marseille F-13009, France.
authors.forename: Emmanuelle
authors.identifier: 0000000276730582
authors.initials: E
authors.lastname: Becker
authors.affiliation: 1] Aix-Marseille University, TAGC, Marseille F-13009, France [2] INSERM UMR_S1090, Marseille F-13009, France [3] CNRS, Marseille F-13009, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Brun
mesh_terms: D030562:Databases, Protein; D006801:Humans; D011485:Protein Binding; D020543:Proteome
chemical_list: D020543:Proteome