title: Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues.
abstract: Adipose tissue growth is associated with preadipocyte proliferation and differentiation. Telomere length is a biological marker for cell proliferation. Preadipocyte factor-1 (pref-1) is specifically expressed in preadipocytes and acts as a molecular gatekeeper of adipogenesis. In the present study, we investigated the fat depot-specific differences in telomere length and pref-1 gene expression in various anatomical sites (subcutaneous, intramuscular and visceral) of fattening Wagyu cattle. Visceral adipose tissue expressed higher pref-1 mRNA than did subcutaneous and intramuscular adipose tissues. The telomere length in visceral adipose tissue tended to be longer than that of subcutaneous and intramuscular adipose tissues. The telomere length of adipose tissue was not associated with adipocyte size from three anatomical sites. No significant correlation was found between the pref-1 mRNA level and the subcutaneous adipocyte size. In contrast, the pref-1 mRNA level was negatively correlated with the intramuscular and visceral adipocyte size. These results suggest that anatomical sites of adipose tissue affect the telomere length and expression pattern of the pref-1 gene in a fat depot-specific manner.
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Nasushiobara-shi, Tochigi-ken 329-2793, Japan. Electronic address: toyamada@affrc.go.jp.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Nasushiobara-shi, Tochigi-ken 329-2793, Japan.
authors.forename: Mikito
authors.initials: M
authors.lastname: Higuchi
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Nasushiobara-shi, Tochigi-ken 329-2793, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Nakanishi
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D016615:Telomere
chemical_list: D017931:DNA Primers; D008565:Membrane Proteins
keywords: Adipose tissue; Cattle; Pref-1; Telomere length