title: Identification of outer membrane Porin D as a vitronectin-binding factor in cystic fibrosis clinical isolates of Pseudomonas aeruginosa.
abstract: BACKGROUND
  Pseudomonas aeruginosa is a pathogen that frequently colonizes patients with cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). Several pathogens are known to bind vitronectin to increase their virulence. Vitronectin has been shown to enhance P. aeruginosa adhesion to host epithelial cells.
  
  
  METHODS
  We screened clinical isolates from the airways of CF patients and from the bloodstream of patients with bacteremia for binding of vitronectin. Two-dimensional SDS-PAGE and a proteomic approach were used to identify vitronectin-receptors in P. aeruginosa.
  
  
  RESULTS
  P. aeruginosa from the airways of CF patients (n=27) bound more vitronectin than bacteremic isolates (n=15, p=0.025). Porin D (OprD) was identified as a vitronectin-binding protein. A P. aeruginosa oprD transposon insertion mutant had a decreased binding to soluble and immobilized vitronectin (p≤0.001).
  
  
  CONCLUSIONS
  P. aeruginosa isolates obtained from CF patients significantly bound vitronectin. Porin D was defined as a novel P. aeruginosa vitronectin-receptor, and we postulate that the Porin D-dependent interaction with vitronectin may be important for colonization.
authors.affiliation: Clinical Microbiology, Department of Translational Medicine, Lund University, Jan Waldenströms gata 59, SE-205 02 Malmö, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Paulsson
authors.affiliation: Clinical Microbiology, Department of Translational Medicine, Lund University, Jan Waldenströms gata 59, SE-205 02 Malmö, Sweden.
authors.forename: Birendra
authors.initials: B
authors.lastname: Singh
authors.affiliation: Clinical Microbiology, Department of Translational Medicine, Lund University, Jan Waldenströms gata 59, SE-205 02 Malmö, Sweden.
authors.forename: Tamim
authors.initials: T
authors.lastname: Al-Jubair
authors.affiliation: Clinical Microbiology, Department of Translational Medicine, Lund University, Jan Waldenströms gata 59, SE-205 02 Malmö, Sweden.
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Su
authors.affiliation: Department of Clinical Microbiology, University Hospital, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Høiby
authors.affiliation: Clinical Microbiology, Department of Translational Medicine, Lund University, Jan Waldenströms gata 59, SE-205 02 Malmö, Sweden. Electronic address: kristian.riesbeck@med.lu.se.
authors.forename: Kristian
authors.initials: K
authors.lastname: Riesbeck
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D015153:Blotting, Western; D003550:Cystic Fibrosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D018272:Porins / Q000096:biosynthesis / Q000235:genetics*; D011552:Pseudomonas Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D012137:Respiratory System / Q000382:microbiology; D019096:Vitronectin / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D018272:Porins; D019096:Vitronectin; C077157:OprD protein, Pseudomonas aeruginosa
keywords: Adhesion molecules; Extracellular matrix; Porin D; Pseudomonas aeruginosa; Vitronectin