title: Altered Expression of Pro-inflammatory Cytokines in Ovarian Follicles of Cows with Cystic Ovarian Disease.
abstract: A growing body of evidence suggests that ovulation shares many of the features of an inflammatory reaction and that cytokines play many diverse and important roles in reproductive biology. The aim of this study was to examine the expression of the pro-inflammatory cytokines interleukin (IL)-1α, IL-6 and tumour necrosis factor (TNF)-α in ovarian cells from cows with cystic ovarian disease (COD) as compared with that in ovarian structures from regularly cycling cows. Expression of genes encoding IL-1α, IL-6 and TNF-α was detected by real-time polymerase chain reaction in follicular cells from ovaries from healthy cows and cows with COD with no significant differences. However, immunohistochemistry showed increased expression of IL-1α, IL-6 and TNF-α in cystic follicles, suggesting that this expression may be related to the persistence of follicular cysts. The effect of COD was evident for IL-1α and TNF-α, and a follicular structure-disease interaction was observed in the expression of all the cytokines evaluated. Thus, altered expression of these proinflammatory cytokines may be related to ovulation failure and development of follicular cysts.
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Baravalle
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina.
authors.forename: A F
authors.initials: AF
authors.lastname: Stassi
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina.
authors.forename: M M L
authors.initials: MM
authors.lastname: Velázquez
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina.
authors.forename: E M
authors.initials: EM
authors.lastname: Belotti
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina.
authors.forename: F M
authors.initials: FM
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina.
authors.forename: H H
authors.initials: HH
authors.lastname: Ortega
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina. Electronic address: salvetti@fcv.unl.edu.ar.
authors.forename: N R
authors.initials: NR
authors.lastname: Salvetti
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000473:pathology*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D007150:Immunohistochemistry; D010048:Ovarian Cysts / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D006080:Ovarian Follicle / Q000276:immunology / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016207:Cytokines
keywords: cow; cytokine; immunohistochemistry; ovarian cyst