title: Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria.
abstract: This paper presents the results of a study to determine the effect of Quillaja saponaria saponins on the lysis of industrial yeast strains. Cell lysis induced by saponin from Q. saponaria combined with the plasmolysing effect of 5% NaCl for Saccharomyces cerevisiae, Kluyveromyces marxianus yeasts biomass was conducted at 50 °C for 24-48 h. Membrane permeability and integrity of the yeast cells were monitored using fluorescent techniques and concentrations of proteins, free amino nitrogen (FAN) and free amino acids in resulting lysates were analyzed. Protein release was significantly higher in the case of yeast cell lysis promoted with 0.008% Q. saponaria and 5% NaCl in comparison to plasmolysis triggered by NaCl only.
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland. Electronic address: joanna.berlowska@p.lodz.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Berlowska
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Dudkiewicz
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kregiel
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Czyzowska
authors.affiliation: Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Witonska
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001329:Autolysis; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology*; D007218:Industrial Microbiology; D007716:Kluyveromyces / Q000187:drug effects / Q000378:metabolism; D059748:Proteolysis / Q000187:drug effects; D031990:Quillaja / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D003902:Detergents; D012503:Saponins; D000641:Ammonia
keywords: Cell lysis; Saponin; Yeast; Yeast extract