title: Mouse genetic background impacts both on iron and non-iron metals parameters and on their relationships.
abstract: Iron is reported to interact with other metals. In addition, it has been shown that genetic background may impact iron metabolism. Our objective was to characterize, in mice of three genetic backgrounds, the links between iron and several non-iron metals. Thirty normal mice (C57BL/6, Balb/c and DBA/2; n = 10 for each group), fed with the same diet, were studied. Quantification of iron, zinc, cobalt, copper, manganese, magnesium and rubidium was performed by ICP/MS in plasma, erythrocytes, liver and spleen. Transferrin saturation was determined. Hepatic hepcidin1 mRNA level was evaluated by quantitative RT-PCR. As previously reported, iron parameters were modulated by genetic background with significantly higher values for plasma iron parameters and liver iron concentration in DBA/2 and Balb/c strains. Hepatic hepcidin1 mRNA level was lower in DBA/2 mice. No iron parameter was correlated with hepcidin1 mRNA levels. Principal component analysis of the data obtained for non-iron metals indicated that metals parameters stratified the mice according to their genetic background. Plasma and tissue metals parameters that are dependent or independent of genetic background were identified. Moreover, relationships were found between plasma and tissue content of iron and some other metals parameters. Our data: (i) confirms the impact of the genetic background on iron parameters, (ii) shows that genetic background may also play a role in the metabolism of non-iron metals, (iii) identifies links between iron and other metals parameters which may have implications in the understanding and, potentially, the modulation of iron metabolism.
authors.affiliation: INSERM UMR 991, CHU Pontchaillou, 35033, Rennes, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Cavey
authors.forename: Martine
authors.initials: M
authors.lastname: Ropert
authors.forename: Marie
authors.initials: M
authors.lastname: de Tayrac
authors.forename: Edouard
authors.initials: E
authors.lastname: Bardou-Jacquet
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Island
authors.forename: Patricia
authors.initials: P
authors.lastname: Leroyer
authors.forename: Claude
authors.initials: C
authors.lastname: Bendavid
authors.forename: Pierre
authors.initials: P
authors.lastname: Brissot
authors.forename: Olivier
authors.initials: O
authors.lastname: Loréal
mesh_terms: D000818:Animals; D003035:Cobalt / Q000097:blood / Q000378:metabolism; D003300:Copper / Q000097:blood / Q000378:metabolism; D000068617:Genetic Background; D064451:Hepcidins / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008274:Magnesium / Q000097:blood / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000097:blood / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics / Q000378:metabolism; D012413:Rubidium / Q000097:blood / Q000378:metabolism; D015032:Zinc / Q000097:blood / Q000378:metabolism
chemical_list: C579599:Hamp protein, mouse; D064451:Hepcidins; D012333:RNA, Messenger; D003035:Cobalt; D008345:Manganese; D003300:Copper; D007501:Iron; D008274:Magnesium; D015032:Zinc; D012413:Rubidium