title: Chinese medicine Jinlida (JLD) ameliorates high-fat-diet induced insulin resistance in rats by reducing lipid accumulation in skeletal muscle.
abstract: The present paper reports the effects of Jinlida (JLD), a traditional Chinese medicine which has been given as a treatment for high-fat-diet (HFD)-induced insulin resistance. A randomized controlled experiment was conducted to provide evidence in support of the affects of JLD on insulin resistance induced by HFD. The affect of JLD on blood glucose, lipid, insulin, adiponectin, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) in serum and lipid content in skeletal muscle was measured. Genes and proteins of the AMPK signaling pathway were analyzed by real time RT-PCR and Western blot. Adiponectin receptor 1 and 2 (ADIPOR1, ADIPOR2) and other genes involved in mitochondrial function and fat oxidation were analyzed by real time RT-PCR. Histological staining was also performed. JLD or pioglitazone administration ameliorated fasting plasma levels of glucose, insulin, triglyceride (TG), total cholesterol (TC), ALT, AST and non-esterified fatty acid (NEFA) (P < 0.05). Treatment with JLD or pioglitazone significantly reverted muscle lipid content (P < 0.05). JLD (1.5 g/kg) significantly increased plasma adiponectin concentration by 60.17% and increased AMPK and acetyl-CoA carboxylase (ACC) phosphorylation in skeletal muscle (P < 0.05). JLD administration increased levels of ADIPOR1 and ADIPOR2 by 1.48 and 1.29 respectively. Levels of genes involved in mitochondrial function and fat oxidation were increased. This study provides the molecular mechanism by which JLD ameliorates HFD-induced insulin resistance in rats.
authors.affiliation: Department of Geratology, The Affiliated Hospital of Hebei University, School of Medicine Baoding 071000, Hebei Province, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zang
authors.affiliation: Department of Clinical Medicine, Shandong University Jinan 250012, Shandong Province, China.
authors.forename: An
authors.initials: A
authors.lastname: Song
authors.affiliation: Department of Internal Medicine, Hebei Medical University Shijiazhuang, 050017, Hebei Province, China.
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Department of Clinical Medical Research Center and Geriatric Key Laboratory, Hebei General Hospital Shijiazhuang 050051, Hebei Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Hebei General Hospital Shijiazhuang 050051, Hebei Province, China.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Song
authors.affiliation: Department of Endocrinology, Bethune International Peace Hospital Shijiazhuang, 050000, Hebei Province, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Hebei General Hospital Shijiazhuang 050051, Hebei Province, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Zhu
authors.affiliation: Department of Endocrinology, Hebei General Hospital Shijiazhuang 050051, Hebei Province, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Internal Medicine, Hebei Medical University Shijiazhuang, 050017, Hebei Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, Hebei Medical University Shijiazhuang, 050017, Hebei Province, China.
authors.forename: Chen-Xi
authors.initials: CX
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, Hebei Medical University Shijiazhuang, 050017, Hebei Province, China.
authors.forename: Jun-Cong
authors.initials: JC
authors.lastname: Kang
authors.affiliation: Department of Endocrinology, Hebei General Hospital Shijiazhuang 050051, Hebei Province, China.
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Ren
keywords: AMP-activated protein kinase; Jinlida (JLD); insulin resistance; lipid accumulation; mitochondria; skeletal muscle