title: Expression of apolipoprotein A-I and A-II in rainbow trout reproductive tract and their possible role in antibacterial defence.
abstract: Antimicrobial proteins such as apolipoproteins A (ApoA-I and ApoA-II) play an important role in the primary defence barrier in vertebrates including fish. The aims of the present study were to isolate and characterise rainbow trout seminal plasma ApoA-I and ApoA-II, to examine the mRNA expression of each apolipoprotein in testis and spermatic ducts, and to test the antibacterial properties of the apolipoproteins. Using a three-step isolation procedure consisting of ion-exchange chromatography, gel filtration and preparative SDS-PAGE, apolipoproteins were purified and identified as ApoA-I and ApoA-II. Both apolipoproteins were represented by several proteoforms. The expression of ApoA-I and ApoA-II mRNA in the reproductive tract and their antibacterial properties against Escherichia coli suggest that seminal apolipoproteins play an important role in innate immunity in the rainbow trout reproductive tract. The functions of seminal ApoA can be related to protection of sperm and reproductive tissue from microbial attack and to the maintenance of sperm membrane integrity.
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland. Electronic address: m.dietrich@pan.olsztyn.pl.
authors.forename: Mariola A
authors.initials: MA
authors.lastname: Dietrich
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Nynca
authors.affiliation: University of Veterinary Medicine in Hanover, Fish Disease Research Unit, Germany.
authors.forename: Mikołaj
authors.initials: M
authors.lastname: Adamek
authors.affiliation: University of Veterinary Medicine in Hanover, Fish Disease Research Unit, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Steinhagen
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Karol
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000235:genetics* / Q000378:metabolism; D016633:Apolipoprotein A-II / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007113:Immunity, Innate; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012661:Semen / Q000737:chemistry; D013737:Testis / Q000378:metabolism; D014649:Vas Deferens / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D029941:Fish Proteins; D012333:RNA, Messenger
keywords: Antimicrobial properties; Apolipoprotein; Rainbow trout; Seminal plasma