title: Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division.
abstract: Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division. In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR tomography. We find the miRNAs have distinct intracellular profiles that resemble two out of the three profiles we previously observed for mRNAs. Our results suggest that miRNAs in addition to proteins and mRNAs may have asymmetric distribution within the oocyte and may contribute to asymmetric cell division as cell fate determinants.
authors.affiliation: 1] Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 142 20 Czech Republic [2] Charles University in Prague, Faculty of Science, Department of Cell Biology, Vinicna 7, Prague, 128 43, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Sidova
authors.affiliation: Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 142 20 Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Sindelka
authors.affiliation: Department of Gastroenterology Hepatology and Infectiology, University Hospital of Dusseldorf, Moorenstrasse 5, Dusseldorf, 40225 Germany.
authors.forename: Mirco
authors.initials: M
authors.lastname: Castoldi
authors.affiliation: EMBL Genomics Core Facility, Meyerhofstr. 1, Heidelberg, D-69117 Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Benes
authors.affiliation: 1] Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 142 20 Czech Republic [2] TATAA Biocenter AB, Odinsgatan 28, Göteborg, 411 03 Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Kubista
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D035683:MicroRNAs