title: A reliable phenotypic assay for detection of ESBLs and AmpCs in MBL-producing gram-negative bacteria with the use of aminophenylboronic acid, dipicolinic acid and cloxacillin.
abstract: ESBLs and AmpCs may escape detection when they coexist with metallo-β-lactamases such as New Delhi Metallo-β-lactamases-1. In this study a combination disk assay was established using cefotaxime, cefotaxime/clavulanic acid, cefotaxime/clavulanic acid/cloxacillin, cefoxitin and cefoxitin/phenylboronic acid/cloxacillin on Mueller Hinton agar supplemented with dipicolinic acid for determination of β-lactamases in the presence of NDM-1.
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
authors.forename: Saswati
authors.initials: S
authors.lastname: Datta
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
authors.forename: Somdatta
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
authors.forename: Shravani
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India. Electronic address: supabasu@yahoo.co.in.
authors.forename: Sulagna
authors.initials: S
authors.lastname: Basu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000494:pharmacology*; D003023:Cloxacillin / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D010641:Phenotype; D010848:Picolinic Acids / Q000494:pharmacology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001897:Boronic Acids; D010848:Picolinic Acids; C028592:3-aminobenzeneboronic acid; D001618:beta-Lactamases; D003023:Cloxacillin; C004860:dipicolinic acid
keywords: Cloxacillin; Combination disk test; Dipicolinic acid; Gram-negative bacteria; NDM-1; Phenylboronic acid