title: Endostatin inhibits bradykinin-induced cardiac contraction.
abstract: Endogenous fragments of extracellular matrix are known to possess various biological effects. Levels of endostatin, a fragment of collagen type XVIII, increase in certain cardiac diseases, such as cardiac hypertrophy and myocardial infarction. However, the influence of endostatin on cardiac contraction has not been clarified. In the present study, we investigated the effects of endostatin on bradykinin-induced atrial contraction. Isometric contractile force of mouse isolated left atria induced by electrical current pulse was measured. Voltage-dependent calcium current of guinea pig ventricular myocytes was measured by a whole-cell patch-clamp technique. Endostatin (100-1,000 ng/ml) alone treatment had no influence on left atrial contraction. On the other hand, pretreatment with endostatin (300 ng/ml) significantly inhibited bradykinin (1 µM)-induced contraction and voltage-dependent calcium current. These data suggest that endostatin may decrease bradykinin-induced cardiac contraction perhaps through the inhibition of voltage-dependent calcium channel.
authors.affiliation: Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Higashi 23 bancho 35-1, Towada city, Aomori 034-8628, Japan.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Yasuda
authors.forename: Lila
authors.initials: L
authors.lastname: Takada
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kajiwara
authors.forename: Muneyosi
authors.initials: M
authors.lastname: Okada
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yamawaki
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D016279:Atrial Function, Left / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels; D004305:Dose-Response Relationship, Drug; D043169:Endostatins / Q000008:administration & dosage / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D009200:Myocardial Contraction / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D011433:Propranolol / Q000494:pharmacology
chemical_list: D000319:Adrenergic beta-Antagonists; D015220:Calcium Channels; D043169:Endostatins; D011433:Propranolol; D001920:Bradykinin; D002118:Calcium