title: Sucrose Transporter AtSUC9 Mediated by a Low Sucrose Level is Involved in Arabidopsis Abiotic Stress Resistance by Regulating Sucrose Distribution and ABA Accumulation.
abstract: Sucrose (Suc) transporters (SUCs or SUTs) are important regulators in plant growth and stress tolerance. However, the mechanism of SUCs in plant abiotic stress resistance remains to be dietermined. Here, we found that AtSUC9 expression was induced by abiotic stress, including salt, osmotic and cold stress conditions. Disruption of AtSUC9 led to sensitive responses to abiotic stress during seed germination and seedling growth. Further analyses indicated that the sensitivity phenotype of Atsuc9 mutants resulted from higher Suc content in shoots and lower Suc content in roots, as compared with that in wild-type (WT) plants. In addition, we found that the expression of AtSUC9 is induced in particular by low levels of exogenous and endogenous Suc, and deletion of AtSUC9 affected the expression of the low Suc level-responsive genes. AtSUC9 also showed an obvious response to treatments with low concentrations of exogenous Suc during seed germination, seedling growth and Suc distribution, and Atsuc9 mutants hardly grew in abiotic stress treatments without exogenous Suc. Moreover, our results illustrated not only that deletion of AtSUC9 blocks abiotic stress-inducible ABA accumulation but also that Atsuc9 mutants had a lower content of endogenous ABA in stress conditions than in normal conditions. Deletion of AtSUC9 also inhibited the expression of many ABA-inducible genes (SnRk2.2/3/6, ABF2/3/4, ABI1/3/4, RD29A, KIN1 and KIN2). These results indicate that AtSUC9 is induced in particular by low Suc levels then mediates the balance of Suc distribution and promotes ABA accumulation to enhance Arabidopsis abiotic stress resistance.
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China These authors contributed equally to this work. Present address: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Wanqiu
authors.initials: W
authors.lastname: Jia
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China These authors contributed equally to this work. Present address: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China These authors contributed equally to this work.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gong
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Cui
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
authors.forename: Na
authors.initials: N
authors.lastname: Cui
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Cao
authors.affiliation: Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China.
authors.forename: Longbing
authors.initials: L
authors.lastname: Rao
authors.affiliation: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture, and Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education, Rice Research Institute, Shenyang Agricultural University, Shenyang, China wangwangche@163.com.
authors.forename: Che
authors.initials: C
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001692:Biological Transport; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009997:Osmotic Pressure; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017384:Sequence Deletion; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D013395:Sucrose / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C078729:sucrose transport protein, plant; D012965:Sodium Chloride; D013395:Sucrose; D000040:Abscisic Acid
keywords: ABA; Abiotic stress; AtSUC9; Low levels of Suc; Suc transporter