title: Tubular cellulose/starch gel composite as food enzyme storehouse.
abstract: The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications. The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area. The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes. X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite. The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Eleftheria
authors.initials: E
authors.lastname: Barouni
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Theano
authors.initials: T
authors.lastname: Petsi
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Kanellaki
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors.forename: Argyro
authors.initials: A
authors.lastname: Bekatorou
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece. Electronic address: a.a.koutinas@upatras.gr.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Koutinas
mesh_terms: D000818:Animals; D002482:Cellulose / Q000737:chemistry*; D012085:Chymosin / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D016062:Porosity; D013213:Starch / Q000737:chemistry*
chemical_list: D002482:Cellulose; D013213:Starch; D012085:Chymosin
keywords: Composite; Milk coagulation; Pore dimensions; Porous cellulose; Rennin immobilization; Starch gel