title: Research of osteoblastic induced rat bone marrow mesenchymal stem cells cultured on β-TCP/PLLA porous scaffold.
abstract: BACKGROUND
  Ceramic and polymer composite scaffolds are widely used in tissue engineering for bone tissue regeneration. Composite of β-tricalcium phosphate (β-TCP) and poly L-lactic acid (PLLA), due to its biocompatibility and biodegradability, is widely used in bioengineering. However, optimal ratio, porosity and pore size of this kind of scaffolds were not very clear yet.
  
  
  METHODS
  We cultured osteoblastic induced rMSCs on β-TCP/PLLA scaffolds to investigate the optimum construction, which owned better properties for supporting cells growth, proliferation and differentiation. A total of 24 mice were divided into three groups: rMSCs + β-TCP/PLLA, osteoblastic rMSCs + β-TCP/PLLA and β-TCP/PLLA without cells. 8 rude mice were implanted with rMSCs + β-TCP/PLLA in the left thighs and β-TCP/PLLA without cells in the right thighs. 8 rude mice were implanted with osteoblastic rMSCs + β-TCP/PLLA in the left thighs and the same treatments in the right thighs as the above. After 8 and 12 weeks, the mice were sacrificed and implants with the surrounding tissues were harvested together. Paraffin sections were got and HE stain and Masson-Goldner stain were employed to observe the ectopic bone formation.
  
  
  RESULTS
  The scaffolds of β-TCP/PLLA = 2:1 significantly increased osteocalcin production of the cells. In addition, scaffolds with NaCl = 70 wt%, pore size 200~450 μm showed better compatibility to these seeding cells. A significantly larger area of bone formation in the osteoblastic rMSCs and β-TCP/PLLA composite than that in rMSCs/scaffold and in the scaffold without cells in vivo.
  
  
  CONCLUSIONS
  compounds of osteoblastic induced rMSCs and the scaffold with β-TCP/PLLA = 2:1, NaCl = 70 wt%, pore size = 200-450 μm had good properties as a kind of bone substitute.
authors.affiliation: Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of West China Basic Medicine and Forensic Medicine, Sichuan University, Laboratory of Cardiovascular Disease, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University Chengdu 610041, China.
authors.forename: Gele
authors.initials: G
authors.lastname: Jin
authors.affiliation: School of West China Basic Medicine and Forensic Medicine, Sichuan University, Laboratory of Cardiovascular Disease, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University Chengdu 610041, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University Chengdu 610041, China.
authors.forename: Cao
authors.initials: C
authors.lastname: Li
keywords: Mesenchymal stem cells; bone tissue engineering; osteoblastic induced; poly-L-lactic acid (PLLA); β-tricalcium phosphate (β-TCP)