title: Ginsenoside Rb2 Attenuates UV-B Radiation-Induced Reactive Oxygen Species and Matrix Metalloproteinase-2 through Upregulation of Antioxidant Components in Human Dermal Fibroblasts.
abstract: OBJECTIVE
  The antiphotoaging activities of ginsenoside Rb2 on the skin, one of the predominant protopanaxadiol-type ginsenosides, were evaluated in cultured human dermal fibroblasts.
  
  
  METHODS
  The antiphotoaging activity was examined by analyzing the levels of reactive oxygen species (ROS), matrix metalloproteinase-2 (MMP-2), total glutathione (GSH) and superoxide dismutase (SOD) activity as well as cell viability for fibroblasts under UV-B irradiation.
  
  
  RESULTS
  When cultured fibroblasts were exposed to Rb2 prior to UV-B irradiation, Rb2 displayed suppressive activities on UV-B-induced ROS elevation and MMP-2 on both activity and protein levels, while it exhibited an enhancing activity on total GSH level and SOD activity diminished by UV-B irradiation. However, Rb2 could not interfere with cell viabilities in UV-B-irradiated fibroblasts.
  
  
  CONCLUSIONS
  Ginsenoside Rb2 plays a photoprotective role against UV-B-induced oxidative stress in human dermal fibroblasts, which implies its skin antiphotoaging potential.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, Korea.
authors.forename: Sun-Joo
authors.initials: SJ
authors.lastname: Oh
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Kim
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D004790:Enzyme Induction / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D036145:Ginsenosides / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D036145:Ginsenosides; D017382:Reactive Oxygen Species; C048026:ginsenoside Rb2; D013482:Superoxide Dismutase; D020778:Matrix Metalloproteinase 2; D005978:Glutathione