title: Comparing mesophilic and thermophilic anaerobic digestion of chicken manure: Microbial community dynamics and process resilience.
abstract: While methane fermentation is considered as the most successful bioenergy treatment for chicken manure, the relationship between operational performance and the dynamic transition of archaeal and bacterial communities remains poorly understood. Two continuous stirred-tank reactors were investigated under thermophilic and mesophilic conditions feeding with 10%TS. The tolerance of thermophilic reactor on total ammonia nitrogen (TAN) was found to be 8000mg/L with free ammonia (FA) 2000mg/L compared to 16,000mg/L (FA1500mg/L) of mesophilic reactor. Biomethane production was 0.29 L/gVSin in the steady stage and decreased following TAN increase. After serious inhibition, the mesophilic reactor was recovered successfully by dilution and washing stratagem compared to the unrecoverable of thermophilic reactor. The relationship between the microbial community structure, the bioreactor performance and inhibitors such as TAN, FA, and volatile fatty acid was evaluated by canonical correspondence analysis. The performance of methanogenic activity and substrate removal efficiency were changed significantly correlating with the community evenness and phylogenetic structure. The resilient archaeal community was found even after serious inhibition in both reactors. Obvious dynamics of bacterial communities were observed in acidogenic and hydrolytic functional bacteria following TAN variation in the different stages.
authors.affiliation: Department of Civil and Environmental Engineering, Graduate School of Engineering Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
authors.forename: Qigui
authors.initials: Q
authors.lastname: Niu
authors.affiliation: Department of Civil and Environmental Engineering, Graduate School of Engineering Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Takemura
authors.affiliation: Department of Civil and Environmental Engineering, Graduate School of Engineering Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kubota
authors.affiliation: Department of Civil and Environmental Engineering, Graduate School of Engineering Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, PR China. Electronic address: yyli@epl1.civil.tohoku.ac.jp.
authors.forename: Yu-You
authors.initials: YY
authors.lastname: Li
mesh_terms: D000641:Ammonia / Q000737:chemistry; D000693:Anaerobiosis; D000818:Animals; D019149:Bioreactors; D002645:Chickens; D005232:Fatty Acids, Volatile / Q000737:chemistry; D005285:Fermentation; D005740:Gases; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008372:Manure; D008697:Methane; D019638:Methanomicrobiaceae / Q000502:physiology*; D017020:Methanosarcina / Q000502:physiology*; D010802:Phylogeny
chemical_list: D005232:Fatty Acids, Volatile; D005740:Gases; D008372:Manure; D000641:Ammonia; D008697:Methane
keywords: Ammonia inhibition; Chicken manure fermentation; Functional resilient; Microbial community dynamic