title: ApoM Suppresses TNF-α-Induced Expression of ICAM-1 and VCAM-1 Through Inhibiting the Activity of NF-κB.
abstract: To explore the anti-inflammatory effect of apolipoprotein M (apoM) on regulation of tumor necrosis factor-α (TNF-α)-induced expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and further investigate the molecular mechanism of apoM in this process. We found that TNF-α could decrease expression of apoM and inhibitor of NF-κB-α (IκBα) in HepG2 cells. Overexpression of apoM caused a significant decrease of ICAM-1 and VCAM-1 expression, while it caused a significant increase of IκBα expression in HepG2 cells. Furthermore, the treatment with TNF-α could increase ICAM-1 and VCAM-1 expression, decrease IκBα protein expression, and increase nuclear factor-κB (NF-κB) activity, and these effects were markedly enhanced by small interfering RNA (siRNA)-mediated silencing of apoM in HepG2 cells. Our findings demonstrated that apoM suppressed TNF-α-induced expression of ICAM-1 and VCAM-1 through inhibiting the activity of NF-κB.
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Ji-Juan
authors.initials: JJ
authors.lastname: Gao
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Hu
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Wang
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Sha
authors.affiliation: 2 Department of Anesthesiology, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Li
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: 3 Department of Vascular Surgery, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Lu
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Chuan
authors.initials: C
authors.lastname: Huang
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D001053:Apolipoproteins / Q000502:physiology*; D000075243:Apolipoproteins M; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism; D054834:Lipocalins / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism
chemical_list: C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D054834:Lipocalins; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1