title: Tissue Distribution, Metabolism, and Excretion of 3,3'-Dichloro-4'-sulfooxy-biphenyl in the Rat.
abstract: Polychlorinated biphenyls (PCBs) with less chlorine atoms exhibit a greater susceptibility to metabolism than their more-chlorinated counterparts. Following initial hydroxylation of these less-chlorinated PCBs, metabolic sulfation to form PCB sulfates is increasingly recognized as an important component of their toxicology. Because procedures for the quantitative analysis of PCB sulfates in tissue samples have not been previously available, we have now developed an efficient, LC-ESI-MS/MS-based protocol for the quantitative analysis of 4-PCB 11 sulfate in biological samples. This procedure was used to determine the distribution of 4-PCB 11 sulfate in liver, kidney, lung, and brain as well as its excretion profile following its intravenous administration to male Sprague-Dawley rats. Following initial uptake of 4-PCB 11 sulfate, its concentration in these tissues and serum declined within the first hour following injection. Although biliary secretion was detected, analysis of 24 h collections of urine and feces revealed recovery of less than 4% of the administered 4-PCB 11 sulfate. High-resolution LC-MS analysis of bile, urine, and feces showed metabolic products derived from 4-PCB 11 sulfate. Thus, 4-PCB 11 sulfate at this dose was not directly excreted in the urine but was instead redistributed to tissues and/or subjected to further metabolism.
authors.affiliation: †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States.
authors.forename: Fabian A
authors.initials: FA
authors.lastname: Grimm
authors.affiliation: †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States.
authors.forename: Xianran
authors.initials: X
authors.lastname: He
authors.affiliation: †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States.
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Teesch
authors.affiliation: †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Lehmler
authors.affiliation: †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States.
authors.forename: Larry W
authors.initials: LW
authors.lastname: Robertson
authors.affiliation: †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Duffel
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid; D007275:Injections, Intravenous; D008297:Male; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D014018:Tissue Distribution
chemical_list: C000604751:3,3'-dichloro-4'-sulfooxy-biphenyl; D011078:Polychlorinated Biphenyls