title: In vitro 3-dimensional tumor model for radiosensitivity of HPV positive OSCC cell lines.
abstract: The incidence of oropharyngeal squamous cell carcinoma (OSCC) is increasing due to the rising prevalence of human papillomavirus (HPV) positive OSCC. HPV positive OSCC is associated with better outcomes than HPV negative OSCC. Our aim was to explore the possibility that this favorable prognosis is due to the enhanced radiosensitivity of HPV positive OSCC. HPV positive OSCC cell lines were generated from the primary OSCCs of 2 patients, and corresponding HPV positive cell lines generated from nodal metastases following xenografting in nude mice. Monolayer and 3 dimensional (3D) culture techniques were used to compare the radiosensitivity of HPV positive lines with that of 2 HPV negative OSCC lines. Clonogenic and protein assays were used to measure survival post radiation. Radiation induced cell cycle changes were studied using flow cytometry. In both monolayer and 3D culture, HPV positive cells exhibited a heterogeneous appearance whereas HPV negative cells tended to be homogeneous. After irradiation, HPV positive cells had a lower survival in clonogenic assays and lower total protein levels in 3D cultures than HPV negative cells. Irradiated HPV positive cells showed a high proportion of cells in G1/S phase, increased apoptosis, an increased proliferation rate, and an inability to form 3D tumor clumps. In conclusion, HPV positive OSCC cells are more radiosensitive than HPV negative OSCC cells in vitro, supporting a more radiosensitive nature of HPV positive OSCC.
authors.affiliation: a Sydney Medical School; Central Clinical School ; The University of Sydney ; Sydney , NSW , Australia.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Barbara
authors.initials: B
authors.lastname: Rose
authors.forename: C Soon
authors.initials: CS
authors.lastname: Lee
authors.forename: Angela M
authors.initials: AM
authors.lastname: Hong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000532:radiotherapy* / Q000821:virology*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D052162:Human papillomavirus 16 / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008819:Mice, Nude; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000473:pathology / Q000532:radiotherapy* / Q000821:virology*; D030361:Papillomavirus Infections / Q000150:complications*; D011836:Radiation Tolerance; D015203:Reproducibility of Results; D023041:Xenograft Model Antitumor Assays
keywords: 3 dimensional, 3D; 3D scaffold; 5-bromo-2 485 0 -deoxy-uridine, BrdU; DNA damage; Gray, Gy; Human papillomavirus, HPV; Immunohistochemistry, IHC; In situ ybridization, ISH; Oropharyngeal cancer; Oropharyngeal squamous cell carcinoma, OSCC; P16INK4, p16; Propodium iodide, PI; Ribonuclease A, RNAse; apoptosis; cell cycle; epithelial-mesenchymal transition, EMT; human papillomavirus; polymerase chain reaction, PCR; radiosensitivity