title: Analgesic and anti-inflammatory effects of hydroalcoholic extract isolated from Semen vaccariae.
abstract: Semen vaccariae, the seeds of Vaccaria segetalis (Neck.) Garcke, is usually used as an important medication for female mammary gland diseases; it has also been used to promote lactation for centuries in China. The purpose of this work was to evaluate the analgesic and anti-inflammatory effects of hydroalcoholic extract from semen vaccariae (HESV) with oral doses of 50, 100 and 200mg/kg•bw in mice and rats. We observed that the HESV could effectively inhibit acetic acid-induced abdominal contraction and could elevate the latency time to thermal stimuli in the hot-plate test in mice. In the xylene-induced ear-swelling test in mice, HESV could suppress the ear swelling. Additionally, HESV could significantly decrease the peritoneal capillary permeability and leukocyte infiltration in mice induced by the intraperitoneal injection of acetic acid. HESV also significantly reduced paw thickness 2-4 hours after the injection of carrageenan in the carrageenan-induced rat paw edema test. This study was the first to demonstrate that the oral administration of HESV might play an important role in the process of analgesia and anti-inflammation, supporting its use for female mammary gland diseases in traditional medicine.
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Dongan
authors.initials: D
authors.lastname: Cui
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Xurong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Dongan
authors.initials: D
authors.lastname: Cui
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Kong
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Li
mesh_terms: D019342:Acetic Acid; D000284:Administration, Oral; D000438:Alcohols / Q000737:chemistry*; D000700:Analgesics / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D006358:Hot Temperature; D008807:Mice, Inbred BALB C; D010146:Pain / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D017288:Pain Threshold / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D012639:Seeds; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D031245:Vaccaria / Q000737:chemistry; D014992:Xylenes
chemical_list: D000438:Alcohols; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012997:Solvents; D014992:Xylenes; D002351:Carrageenan; D019342:Acetic Acid