title: Diacylglyceryltrimethylhomoserine content and gene expression changes triggered by phosphate deprivation in the mycelium of the basidiomycete Flammulina velutipes.
abstract: Diacylglyceryltrimethylhomoserines (DGTS) are betaine-type lipids that are phosphate-free analogs of phosphatidylcholines (PC). DGTS are abundant in some bacteria, algae, primitive vascular plants and fungi. In this study, we report inorganic phosphate (Pi) deficiency-induced DGTS synthesis in the basidial fungus Flammulina velutipes (Curt.: Fr.) Sing. We present results of an expression analysis of the BTA1 gene that codes for betaine lipid synthase and two genes of PC biosynthesis (CHO2 and CPT1) during phosphate starvation of F. velutipes culture. We demonstrate that FvBTA1 gene has increased transcript abundance under phosphate starvation. Despite depletion in PC, both CHO2 and CPT1 were determined to have increased expression. We also describe the deduced amino acid sequence and genomic structure of the BTA1 gene in F. velutipes. Phylogenetic relationships between putative orthologs of BTA1 proteins of basidiomycete fungi are discussed.
authors.affiliation: Komarov Botanical Institute, Russian Academy of Sciences, 2 Professor Popov str., St. Petersburg 197376, Russia. Electronic address: senik@binran.ru.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Senik
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina str., Moscow 119991, Russia.
authors.forename: Liliya G
authors.initials: LG
authors.lastname: Maloshenok
authors.affiliation: Komarov Botanical Institute, Russian Academy of Sciences, 2 Professor Popov str., St. Petersburg 197376, Russia.
authors.forename: Ekaterina R
authors.initials: ER
authors.lastname: Kotlova
authors.affiliation: Komarov Botanical Institute, Russian Academy of Sciences, 2 Professor Popov str., St. Petersburg 197376, Russia.
authors.forename: Alexey L
authors.initials: AL
authors.lastname: Shavarda
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, 33 Leninsky pr., Moscow 117071, Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Moiseenko
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina str., Moscow 119991, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Bruskin
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, 33 Leninsky pr., Moscow 117071, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Koroleva
authors.affiliation: Komarov Botanical Institute, Russian Academy of Sciences, 2 Professor Popov str., St. Petersburg 197376, Russia.
authors.forename: Nadezhda V
authors.initials: NV
authors.lastname: Psurtseva
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055393:Flammulina / Q000378:metabolism*; D015870:Gene Expression; D006017:Glycolipids / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D025282:Mycelium / Q000187:drug effects*; D010710:Phosphates / Q000032:analysis / Q000378:metabolism; D010713:Phosphatidylcholines / Q000032:analysis; D010802:Phylogeny; D014280:Triglycerides / Q000032:analysis* / Q000235:genetics
chemical_list: C021660:1(3),2-diacylglyceryl-3(1)-O-4'-(N,N,N-trimethyl)homoserine; D006017:Glycolipids; D010710:Phosphates; D010713:Phosphatidylcholines; D014280:Triglycerides
keywords: BTA1; Basidiomycetes; Betaine lipid; CHO2; CPT1; Flammulina velutipes; Gene expression; Glycerolipid metabolism; Phosphate starvation; Phylogenetic analysis