title: Diagnostic value of a plasma microRNA signature in gastric cancer: a microRNA expression analysis.
abstract: The differential expression of microRNAs (miRNAs) in plasma of gastric cancer (GC) patients may serve as a diagnostic biomarker. A total of 33 miRNAs were identified through the initial screening phase (3 GC pools vs. 1 normal control (NC) pool) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel (miRCURY-Ready-to-Use-PCR-Human-panel-I + II-V1.M). By qRT-PCR, these miRNAs were further assessed in training (30 GC VS. 30 NCs) and testing stages (71 GC VS. 61 NCs). We discovered a plasma miRNA signature including five up-regulated miRNAs (miR-185, miR-20a, miR-210, miR-25 and miR-92b), and this signature was evaluated to be a potential diagnostic marker of GC. The areas under the receiver operating characteristic curve of the signature were 0.86, 0.74 and 0.87 for the training, testing and the external validation stages (32 GC VS. 18 NCs), respectively. The five miRNAs were consistently dysregulated in GC tissues (n = 30). Moreover, miR-185 was decreased while miR-20a, miR-210 and miR-92b were increased in arterial plasma (n = 38). However, none of the miRNAs in the exosomes showed different expression between 10 GC patients and 10 NCs. In conclusion, we identified a five-miRNA signature in the peripheral plasma which could serve as a non-invasive biomarker in detection of GC.
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Pathology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Oncology, Clinical Medical College of Yangzhou University, No. 98 Nantong Western road, Yangzhou 225001, PR China.
authors.forename: Yinxia
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China.
authors.forename: Lianwen
authors.initials: L
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Emergency, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, No. 22 Hankou Road, Nanjing 210093, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Tongshan
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Oncology, Third Affiliated Hospital of Soochow University, 185 Juqian Road, Changzhou 213003, PR China.
authors.forename: Danxia
authors.initials: D
authors.lastname: Zhu
authors.affiliation: 1] Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China [2] Cancer Center of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Shu
authors.affiliation: 1] Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China [2] Cancer Center of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D035683:MicroRNAs