title: The Effect of Epigallocatechin Gallate on the Dentin Bond Durability of Two Self-etch Adhesives.
abstract: BACKGROUND
  Self-etch adhesives can activate matrix metalloproteinase (MMP) which hydrolyzes organic matrix of demineralized dentin. Epigallocatechin gallate (EGCG), especially found in green tea, could inhibit the activation of MMP.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the effect of adding Epigallocatechin gallate (EGCG) into two types of adhesives on dentin bond strength.
  
  
  METHODS
  In this experimental study, 64 extracted third molars were randomly divided into 16 groups. Clearfil SE Bond and Filtek Silorane System with 0 µM, 25µM, 50µM, and 100µM concentration of 95% EGCG were used for bonding. Following the bonding and fabrication of beams (1±0.1 mm(2)) and storage in distilled water, the specimens were subjected to thermal cycles. Microtensile bond strengths of 8 groups were examined after 24 hours and others were tested after 6 months. The fracture modes of specimens were evaluated by stereomicroscope and SEM. Data were analyzed by three-way ANOVA and t-test (α = 0.05).
  
  
  RESULTS
  The results of the three- way ANOVA test showed that types of bonding, storage time and interactive effect of EGCG concentration and bonding influenced the bond strength of specimens significantly (p<0.05). The results of the t-test indicated that storage time only had significant effect on bond strength of Clearfil SE Bond with no EGCG (p= 0.017). The most common failure modes in Filtek Silorane System groups and Clearfil SE Bond groups were adhesive and mixed/cohesive, respectively. The results of SEM at different magnifications showed that most fractures have occurred in the hybrid layer.
  
  
  CONCLUSIONS
  Although adding 100 µM volume of EGCG to Clearfil SE Bond can preserve the dentin bond, incorporation of EGCG in the silorane system, especially in high concentrations, decreases the bond strength after 6 months.
authors.affiliation: Dental Research Center, Dept. of Operative Dentistry, School of Dentistry, Hamedan University of Medical Sciences, Hamedan, Iran;
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khamverdi
authors.affiliation: Dept. of Operative Dentistry, School of Dentistry, Gilan University of Medical Sciences,Gilan, Iran;
authors.forename: Loghman
authors.initials: L
authors.lastname: Rezaei-Soufi
authors.affiliation: Dept. of Operative Dentistry, School of Dentistry, Gilan University of Medical Sciences,Gilan, Iran;
authors.forename: Tayebeh
authors.initials: T
authors.lastname: Rostamzadeh
keywords: Adhesives; Dentin; Epigallocatechin Gallate; Matrix Metalloproteinase