title: Increased 17ß-hydroxysteroid dehydrogenase type 1 levels in primary cervical cancer.
abstract: Infections with oncogenic human papillomavirus (HPV) strains are recognized as the major risk factor for developing malignant lesions in the uterine cervix. However, several findings have demonstrated cooperation between HPV infection and 17β-estradiol (E2) in cervical carcinogenesis. The 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1) is the enzyme involved in the transformation of estrone (E1) into E2. In this study, we identified the HSD17B1 transcript and protein in HeLa, SiHa, Ca Ski and C-33A cervical cancer cells. These cells were able to convert E1 to E2 in a time-dependent manner. Moreover, we identified the HSD17B1 transcript and protein in primary cancerous tissues (n=28) and in histologically unchanged tissues (n=25). We did not observe significant differences (P=0.33) between the HSD17B1 transcript levels in cancerous tissues and histologically unchanged tissues. However, we found an overrepresentation of the HSD17B1 protein in cancerous tissues compared with histologically unchanged tissues (P<0.001). This overrepresentation of the HSD17B1 protein in primary cervical cancerous tissues may be responsible for the local conversion of E1 to E2.
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland; The Centre for Orthodontic Mini-implants of the Chair and Clinic of Maxillofacial Orthopedics and Orthodontics, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Tomaszewska
authors.affiliation: Department of Electroradiology, Poznan University of Medical Sciences, Poznan, Poland; Department of Radiotherapy and Gynecological Oncology, Greater Poland Cancer Centre, Poznan, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Roszak
authors.affiliation: Division of Gynecological Surgery, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Pawlik
authors.affiliation: Division of Gynecological Surgery, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Sajdak
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: pjagodzi@am.poznan.pl.
authors.forename: Paweł Piotr
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D045744:Cell Line, Tumor; D004958:Estradiol / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D004970:Estrone; D004958:Estradiol; D004960:Estradiol Dehydrogenases; C513331:HSD17B1 protein, human
keywords: 17β-Estradiol; Cervical cancer; HSD17B1