title: Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.
abstract: BACKGROUND
  Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices.
  
  
  RESULTS
  For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices.
  
  
  CONCLUSIONS
  A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gonzalez
authors.affiliation: 2Duke Clinical Research Institute, Duke University Medical Center, Durham, NC, USA.
authors.forename: Chiara
authors.initials: C
authors.lastname: Melloni
authors.affiliation: 3Department of Pediatrics, Riley Hospital for Children, Indiana University, Indianapolis, IN, USA.
authors.forename: Brenda B
authors.initials: BB
authors.lastname: Poindexter
authors.forename: Ram
authors.initials: R
authors.lastname: Yogev
authors.affiliation: 5Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Atz
authors.affiliation: 6Kosair Charities Pediatric Clinical Research Unit, Department of Pediatrics, University of Louisville, Kosair Children's Hospital, Louisville, KY, USA.
authors.forename: Janice E
authors.initials: JE
authors.lastname: Sullivan
authors.affiliation: 7Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Susan R
authors.initials: SR
authors.lastname: Mendley
authors.affiliation: 8Department of Neonatology, Wesley Medical Center, Wichita, KS, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Delmore
authors.affiliation: 9OpAns, LLC, 4134 S Alston Ave # 101, Durham, NC 27713, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Delinsky
authors.affiliation: 2Duke Clinical Research Institute, Duke University Medical Center, Durham, NC, USA.
authors.forename: Kanecia
authors.initials: K
authors.lastname: Zimmerman
authors.affiliation: 10EMMES Corp., 401 N Washington St # 700, Rockville, MD 20850, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Lewandowski
authors.affiliation: 2Duke Clinical Research Institute, Duke University Medical Center, Durham, NC, USA.
authors.forename: Barrie
authors.initials: B
authors.lastname: Harper
authors.affiliation: 9OpAns, LLC, 4134 S Alston Ave # 101, Durham, NC 27713, USA.
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Lewis
authors.affiliation: 2Duke Clinical Research Institute, Duke University Medical Center, Durham, NC, USA.|11Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Benjamin
authors.affiliation: 2Duke Clinical Research Institute, Duke University Medical Center, Durham, NC, USA.|11Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Cohen-Wolkowiez
mesh_terms: D000328:Adult; D002138:Calibration; D002648:Child; D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing / Q000379:methods*; D006801:Humans; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000097:blood* / Q000652:urine*; D016482:Urinalysis / Q000379:methods*
chemical_list: D015662:Trimethoprim, Sulfamethoxazole Drug Combination