title: Identification of innovative potential quality markers in rocket and melon fresh-cut produce.
abstract: Ready-to-eat fresh cut produce are exposed to pre- and postharvest abiotic stresses during the production chain. Our work aimed to identify stress responsive genes as new molecular markers of quality that can be widely applied to leaves and fruits and easily determined at any stage of the production chain. Stress responsive genes associated with quality losses were isolated in rocket and melon fresh-cut produce and their expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time points after harvest at 20 °C and 4 °C. qRT-PCR results were supported by correlation analysis with physiological and biochemical determinations evaluated at the same conditions such as chlorophyll a fluorescence indices, total, reducing sugars, sucrose, ethylene, ascorbic acid, lipid peroxidation and reactive oxygen species. In both species the putative molecular markers increased their expression soon after harvest suggesting a possible use as novel and objective quality markers of fresh-cut produces.
authors.affiliation: Institut de Biologie Physico-Chimique, 13 Rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Cavaiuolo
authors.affiliation: Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, via Celoria 2, Milano 20133, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Cocetta
authors.affiliation: Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, via Celoria 2, Milano 20133, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Bulgari
authors.affiliation: Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, via Celoria 2, Milano 20133, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Spinardi
authors.affiliation: Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, via Celoria 2, Milano 20133, Italy. Electronic address: antonio.ferrante@unimi.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ferrante
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D019662:Cucurbitaceae / Q000737:chemistry*; D005030:Ethylenes / Q000032:analysis; D063427:Food Quality; D005638:Fruit / Q000737:chemistry*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D018515:Plant Leaves / Q000737:chemistry*; D018749:RNA, Plant / Q000235:genetics; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D002241:Carbohydrates; D005030:Ethylenes; D018749:RNA, Plant; D017392:Thiobarbituric Acid Reactive Substances; D002734:Chlorophyll; C036216:ethylene; D001205:Ascorbic Acid; D000077194:Chlorophyll A
keywords: Abiotic stress; Ascorbic acid (PubChem CID: 54670067); Chlorophyll (PubChem CID: 6477652); Ethylene (PubChem CID: 6325); Fresh-cut vegetables; Fructose (PubChem CID: 53782691); Galactose (PubChem CID: 439357); Glucose (PubChem CID: 53782692); Hydrogen peroxide (PubChem CID: 784); Malondialdehyde (PubChem CID: 10964); Postharvest; Production chain; Senescence; Sucrose (PubChem CID: 5988); Superoxide anion (PubChem CID: 5359597)