title: Endothelial nitric oxide synthase Glu298Asp gene polymorphism influences body composition and biochemical parameters but not the nitric oxide response to eccentric resistance exercise in elderly obese women.
abstract: Both endothelial nitric oxide synthase (eNOS) gene polymorphism and nitric oxide (NO) are involved in important cardiovascular, muscular and inflammatory physiological mechanisms during ageing and response to exercise. The aim of this study was to investigate the NO kinetic response following an acute eccentric resistance exercise (ERE) session and the possible effect of the Glu298Asp eNOS gene polymorphism in elderly obese women. Eighty-seven women (age 69·4 ± 6·1 years, body weight 74·9 ± 12·7 kg, height 151·9 ± 6·0 cm and BMI 32·5 ± 5·7 kg m-2) completed seven sets of ten eccentric repetitions at 110% of the ten repetitions maximum (10RM). NO concentrations remained elevated up to 48 h following the acute ERE session as compared with baseline, for GG and GT/TT groups (P<0·05), with no differences between genotypes. The GG genotype group had higher body weight, prevalence of obesity (BMI classification - 81% versus 56%), BMI and higher relative muscle strength, while they had significantly lower triglycerides, VLDL and urea concentrations as compared with TT/TG group. In conclusion, NO remains elevated for up to 48 h after an acute ERE session, without genotype interaction. The TT/TG genotype had a negative impact on triglycerides, VLDL and urea concentrations. Thus, T carriers should increase their attention to cardiovascular risk factor and metabolic disorders.
authors.affiliation: Catholic University of Brasilia, Graduation Program on Physical Education, Brasilia, Brazil.
authors.forename: Tatiane Gomes
authors.initials: TG
authors.lastname: Teixeira
authors.affiliation: Catholic University of Brasilia, Graduation Program on Physical Education, Brasilia, Brazil.
authors.forename: Ramires Alsamir
authors.initials: RA
authors.lastname: Tibana
authors.affiliation: Catholic University of Brasilia, Graduation Program on Physical Education, Brasilia, Brazil.
authors.forename: Dahan da Cunha
authors.initials: DD
authors.lastname: Nascimento
authors.affiliation: Laboratory of Exercise Physiology, Faculty Estacio of Vitoria, Vitoria, ES, Brazil.
authors.forename: Nuno Manuel Frade
authors.initials: NM
authors.lastname: de Sousa
authors.affiliation: Catholic University of Brasilia, Graduation Program on Physical Education, Brasilia, Brazil.|University of Brasilia, Brasilia, Brazil.
authors.forename: Vinicius Carolino
authors.initials: VC
authors.lastname: de Souza
authors.affiliation: Universitary Center of Brasilia (UDF), Brazil.
authors.forename: Denis César Leite
authors.initials: DC
authors.lastname: Vieira
authors.affiliation: University of Brasilia, Brasilia, Brazil.
authors.forename: Otávio de Toledo
authors.initials: OT
authors.lastname: Nóbrega
authors.affiliation: Department of Physical Education of Federal University of Mato Grosso do Sul, Brazil.
authors.forename: Jeeser Alves
authors.initials: JA
authors.lastname: de Almeida
authors.affiliation: Department of Kinesiology and Nutrition Sciences, University of Nevada, Las Vegas, NV, USA.
authors.forename: James
authors.initials: J
authors.lastname: Navalta
authors.affiliation: Catholic University of Brasilia, Graduation Program on Physical Education, Brasilia, Brazil. jonatop@gmail.com.
authors.forename: Jonato
authors.initials: J
authors.lastname: Prestes
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001823:Body Composition / Q000235:genetics*; D015992:Body Mass Index; D015444:Exercise; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007700:Kinetics; D008079:Lipoproteins, VLDL / Q000097:blood; D008875:Middle Aged; D053580:Muscle Strength; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology; D010641:Phenotype; D011110:Polymorphism, Genetic; D015995:Prevalence; D055070:Resistance Training; D012307:Risk Factors; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood; D014508:Urea / Q000097:blood
chemical_list: D015415:Biomarkers; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D009569:Nitric Oxide; D014508:Urea; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: ageing; eccentric training; genetics; obesity; strength training