title: Antimicrobial Hyaluronic Acid/Poly(amidoamine) Dendrimer Multilayer on Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Prepared by a Layer-by-Layer Self-Assembly Method.
abstract: In this article, we prepared hyaluronic acid/poly(amidoamine) dendrimer (HA/PAMAM) multilayers on a poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-4HB)] substrate by a layer-by-layer self-assembly method for antimicrobial biomaterials. The results of ζ potential and quartz crystal microbalance with dissipation (QCM-D) showed that HA/PAMAM multilayers could be formed on the substrate layer by layer. We used QCM-D to show that both the HA outer layer and the PAMAM outer layer exhibited good protein-resistant activity to bovine serum albumin and bacterial antiadhesion activity to Escherichia coli. By a live/dead assay and the colony counting method, we found that the PAMAM outer layer could also exhibit bactericidal activity against E. coli, while the HA outer layer had no bactericidal activity. Both the bacterial antiadhesion activity and the bactericidal activity of the samples could be maintained even after storage in phosphate-buffered saline for up to 14 days. An in vitro MTT assay showed that the multilayers had no cytotoxicity to L929 cells, and HA molecules in the multilayers could improve the biocompatibility of the film.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.|National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.|Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Jiezhao
authors.initials: J
authors.lastname: Zhan
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.|National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.|Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.|National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.|Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Sa
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.|National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.|Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.|National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.|Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.|National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.|Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D000094122:Polyhydroxybutyrates
chemical_list: D000890:Anti-Infective Agents; D050091:Dendrimers; D006820:Hyaluronic Acid; D006885:Hydroxybutyrates; D011091:Polyesters; C104700:PAMAM Starburst; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates
keywords: PAMAM dendrimer; QCM-D; antimicrobial activity; hyaluronic acid; layer by layer