title: Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma.
abstract: BACKGROUND
  Lung adenocarcinoma (LAC), the primary histological type of non-small cell lung cancer (NSCLC), has displayed an increasing incidence and mortality worldwide. However, therapeutic approaches were limited. Dysregulation of some lncRNAs has been shown in various types of cancers including LAC. The aim of the present study was to vertify lncRNA DLX6-AS1 expression in LAC.
  
  
  METHODS
  Microarray assay revealed expression profile of lncRNAs in LAC. qRT-PCR ( quantitative reverse transcription PCR) was performed to identify lncRNA DLX6-AS1 expression level in 72 paired LAC and adjacent normal lung tissues. qRT-PCR and Western blotting were used to verify that down-regulation lncRNA DLX6-AS1 decreased DLX6 (distal-less homeobox 6) mRNA and protein expression.
  
  
  RESULTS
  Microarray analysis identified up-regulation of 272 lncRNAs and down-regulation of 635 lncRNAs in LAC tissues. The expression level of lncRNA DLX6-AS1 in LAC tissues was significantly higher compared to paired adjacent normal lung tissues (P< 0.05). In addition, its expression level was closed correlated with both histological differentiation (P = 0.004) and TNM stage (P = 0.033). qRT-PCR and Western blotting analysis showed that DLX6 mRNA and protein levels were lower in si-LncRNA group than in the NC (negative control) and Blank groups.
  
  
  CONCLUSIONS
  Microarray analysis identified that lncRNA DLX6-AS1 was up-regulated in LAC tissues. High DLX6-AS1 expression levels were significantly associated with both histological differentiation and TNM stage. Down-regulation of lncRNA DLX6-AS1 expression decreased the DLX6 mRNA and protein levels.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Shaozhi
authors.initials: S
authors.lastname: Dun
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
keywords: DLX6; LAC; Microarray assay; lncRNA DLX6-AS1