title: An anionic conjugated polymer as a multi-action sensor for the sensitive detection of Cu(2+) and PPi, real-time ALP assaying and cell imaging.
abstract: A Cu(2+) ensemble polyfluorene derivative, poly[5,5'-(((9H-fluorene-9,9-diyl)bis(hexane-6,1-diyl))bis(oxy))diisophthalate] sodium salt (PFT), displays unprecedented selectivity for PPi (LOD = 2.26 ppb) in aqueous solution as well as in random urine samples at physiological pH vis-a-vis monitoring ALP activity. Furthermore, intracellular imaging of Cu(2+) and PPi in mouse macrophage (J774A.1) and human breast cancer cells (MDA-MB231) was achieved to confirm the viability of PFT in biological systems.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati-781039, India. pki@iitg.ernet.in.
authors.forename: Akhtar H
authors.initials: AH
authors.lastname: Malik
authors.forename: Sameer
authors.initials: S
authors.lastname: Hussain
authors.forename: Arvin Sain
authors.initials: AS
authors.lastname: Tanwar
authors.forename: Sourav
authors.initials: S
authors.lastname: Layek
authors.forename: Vishal
authors.initials: V
authors.lastname: Trivedi
authors.forename: Parameswar K
authors.initials: PK
authors.lastname: Iyer
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D000838:Anions; D045744:Cell Line, Tumor; D003199:Computer Systems; D003300:Copper / Q000032:analysis*; D011756:Diphosphates / Q000652:urine*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008264:Macrophages / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D011108:Polymers / Q000737:chemistry*
chemical_list: D000838:Anions; D011756:Diphosphates; D005456:Fluorescent Dyes; D011108:Polymers; D003300:Copper; D000469:Alkaline Phosphatase