title: FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver.
abstract: In this work we apply FT-IR imaging of large areas of liver tissue cross-section samples (∼5 cm × 5 cm) for quantitative assessment of steatosis in murine model of Non-Alcoholic Fatty Liver (NAFLD). We quantified the area of liver tissue occupied by lipid droplets (LDs) by FT-IR imaging and Oil Red O (ORO) staining for comparison. Two alternative FT-IR based approaches are presented. The first, straightforward method, was based on average spectra from tissues and provided values of the fat content by using a PLS regression model and the reference method. The second one – the chemometric-based method – enabled us to determine the values of the fat content, independently of the reference method by means of k-means cluster (KMC) analysis. In summary, FT-IR images of large size liver sections may prove to be useful for quantifying liver steatosis without the need of tissue staining.
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Krakow, Poland. baranska@chemia.uj.edu.pl.
authors.forename: K
authors.initials: K
authors.lastname: Kochan
authors.forename: E
authors.initials: E
authors.lastname: Maslak
authors.forename: S
authors.initials: S
authors.lastname: Chlopicki
authors.forename: M
authors.initials: M
authors.lastname: Baranska
mesh_terms: D000818:Animals; D005223:Fats / Q000032:analysis*; D008099:Liver / Q000737:chemistry* / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005223:Fats