title: Sporicidal Activities of Various Surfactant Components against Bacillus subtilis Spores.
abstract: The sporicidal activities against Bacillus subtilis spores of surfactant components with hydrophilic and hydrophobic properties that can lead to the denaturation of various proteins comprising the spore structure were investigated. The reduction in spore numbers by each of the surfactant components bornyl acetate, geranyl acetate, pinene, p-cymene, camphene, citral, 2,3-dihydrobenzofuran, polylysine, and thiamine dilaurylsulfate at 1% was estimated at 1 to 2 log CFU/ml. The average hydrophilelipophile balance value of surfactants with sporicidal activity causing a reduction of 1 to 2 log CFU/ml was 9.3, with a range from 6.7 to 15.8, which is similar to the values of various chemical surfactants of 9.6 to 16.7. The results also showed that the surfactants that were hydrophobic were more effective than those that were hydrophilic in killing B. subtilis spores. Furthermore, the sporicidal effect of surfactants like geranyl acetate and γ-terpinene was significantly enhanced in the presence of a germinant, because L-alanine and synergistic cofactors (e.g., K(+) ions) trigger cortex hydrolysis in spores.
authors.affiliation: CJ Foods R&D, CJCheiljedang Corp., 518 Gyeongin-ro, Guro-gu, Seoul 152-050, South Korea.
authors.forename: Won-Il
authors.initials: WI
authors.lastname: Cho
authors.affiliation: Department of Food and Food Service Industry, Kyungpook National University, Sangju 742-711, South Korea.
authors.forename: Chan-Ick
authors.initials: CI
authors.lastname: Cheigh
authors.affiliation: Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, South Korea.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Hwang
authors.affiliation: Department of Food Science and Engineering, Ewha Womans University, Seoul 120-750, South Korea. mschung@ewha.ac.kr.
authors.forename: Myong-Soo
authors.initials: MS
authors.lastname: Chung
mesh_terms: D000409:Alanine / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects*; D015169:Colony Count, Microbial; D004203:Disinfection; D005516:Food Microbiology; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions; D011188:Potassium / Q000737:chemistry; D013171:Spores, Bacterial / Q000187:drug effects*; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature
chemical_list: D000890:Anti-Infective Agents; D007477:Ions; D013501:Surface-Active Agents; D000409:Alanine; D011188:Potassium