title: Stigmasterol protects against Ang II-induced proliferation of the A7r5 aortic smooth muscle cell-line.
abstract: Excessive proliferation of vascular smooth muscle cells is a crucial event in the pathogenesis of several cardiovascular diseases, including atherosclerosis and restenosis. In this study, we reported that stigmasterol was effective in inhibiting vascular cell proliferation exemplified by using A7r5 cells stimulated by Ang II. Mechanism analysis showed that this inhibiting effect mainly occurred by the arrest of the cell-cycle and promotion of apoptosis. In addition, stigmasterol inhibition effects were detected in association with decreased ROS production, enhanced SOD and CAT activity, decreased abundance of cyclin A, CDK2, PCNA, bax and bcl-2, and increased levels of p53 protein. Our study provided implications for the development of therapeutic strategies to protect against certain cardiovascular pathologies, such as atherosclerosis and restenosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical College, Guangzhou, China. luqun2@126.com.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Xie
authors.forename: Qun
authors.initials: Q
authors.lastname: Lu
authors.forename: Shaohong
authors.initials: S
authors.lastname: Luo
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000166:cytology* / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction; D013265:Stigmasterol / Q000494:pharmacology*
chemical_list: D019925:Cyclin A; D018809:Proliferating Cell Nuclear Antigen; D020011:Protective Agents; D000804:Angiotensin II; D013265:Stigmasterol; D051357:Cyclin-Dependent Kinase 2