title: Determination of glucosamine and its derivatives released from photocrosslinked gelatin hydrogels using HPLC.
abstract: A simple, accurate and validated reverse-phase high-performance liquid chromatography (HPLC)/UV method is developed for the determination of glucosamine hydrochloride (GlcN), N-acetyl-glucosamine (NAG) and N-acryloyl-glucosamine (AGA) released from photocrosslinked gelatin hydrogels. The HPLC separation was achieved on a Shimadzu InertSustain amino column (250 × 4.6 mm, 5 µm particle size) at room temperature using a mobile phase of acetonitrile-phosphate buffer (75:25, v/v, pH 6.0) at a flow rate of 1.0 mL/min and UV detection of 194 nm. The method was validated for specificity, linearity, limit of detection and quantification, accuracy, precision, extraction recovery and solution stability. The calibration curves were with excellent linearity, with correlation coefficients (R(2)) >0.999 for all three drugs. The intra- and inter-day variation was <3.10% and the relative error was between -1.43 and 1.78%. The extraction recovery results ranged from 94.62 to 99.33%, demonstrating the absence of matrix effect. The sample and standard solutions were stable for more than 2 months. The method was successfully used for the analysis of released properties of drugs physically encapsulated and chemically crosslinked in the gelatin hydrogels.
authors.affiliation: Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, 310027, China.|Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Hangzhou, China.
authors.forename: Hairui
authors.identifier: http://orcid.org/0000-0002-6512-7091
authors.initials: H
authors.lastname: Suo
authors.affiliation: Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, 310027, China.|Qiushi Academy for Advanced Studies, Zhejiang University, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Hangzhou, China.
authors.forename: Kedi
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, 310027, China.|Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Hangzhou, China.
authors.forename: Hengyi
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, 310027, China.|Qiushi Academy for Advanced Studies, Zhejiang University, Hangzhou, China.|Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Hangzhou, China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Zheng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005780:Gelatin / Q000737:chemistry*; D005944:Glucosamine / Q000032:analysis*; D020100:Hydrogels / Q000737:chemistry*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D020100:Hydrogels; D005780:Gelatin; D005944:Glucosamine
keywords: HPLC; controlled release; glucosamine; hydrogel; photocrosslink