title: Single-molecule insights into mRNA dynamics in neurons.
abstract: Targeting of mRNAs to neuronal dendrites and axons plays an integral role in intracellular signaling, development, and synaptic plasticity. Single-molecule imaging of mRNAs in neurons and brain tissue has led to enhanced understanding of mRNA dynamics. Here we discuss aspects of mRNA regulation as revealed by single-molecule detection, which has led to quantitative analyses of mRNA diversity, localization, transport, and translation. These exciting new discoveries propel our understanding of the life of an mRNA in a neuron and how its activity is regulated at the single-molecule level.
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Adina R
authors.initials: AR
authors.lastname: Buxbaum
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Young J
authors.initials: YJ
authors.lastname: Yoon
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Singer
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA; Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea; Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea. Electronic address: hyeyoon.park@snu.ac.kr.
authors.forename: Hye Yoon
authors.initials: HY
authors.lastname: Park
mesh_terms: D000818:Animals; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007425:Intracellular Membranes / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D014176:Protein Biosynthesis / Q000502:physiology; D012333:RNA, Messenger / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D012333:RNA, Messenger
keywords: RNA localization; local translation; mRNA tracking; smFISH