title: Cardioprotective Effect of Licochalcone D against Myocardial Ischemia/Reperfusion Injury in Langendorff-Perfused Rat Hearts.
abstract: Flavonoids are important components of 'functional foods', with beneficial effects on cardiovascular function. The present study was designed to investigate whether licochalcone D (LD) could be a cardioprotective agent in ischemia/reperfusion (I/R) injury and to shed light on its possible mechanism. Compared with the I/R group, LD treatment enhanced myocardial function (increased LVDP, dp/dtmax, dp/dtmin, HR and CR) and suppressed cardiac injury (decreased LDH, CK and myocardial infarct size). Moreover, LD treatment reversed the I/R-induced cleavage of caspase-3 and PARP, resulting in a significant decrease in proinflammatory factors and an increase in antioxidant capacity in I/R myocardial tissue. The mechanisms underlying the antiapoptosis, antiinflammation and antioxidant effects were related to the activation of the AKT pathway and to the blockage of the NF-κB/p65 and p38 MAPK pathways in the I/R-injured heart. Additionally, LD treatment markedly activated endothelial nitric oxide synthase (eNOS) and reduced nitric oxide (NO) production. The findings indicated that LD had real cardioprotective potential and provided support for the use of LD in myocardial I/R injury.
authors.affiliation: The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yuan
authors.affiliation: The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong, China.
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Niu
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832002, Xinjiang, China.
authors.forename: Peng-long
authors.initials: PL
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832002, Xinjiang, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong, China.
authors.forename: Shi-han
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Binzhou Medical College, Yantai, 264000, Shandong, China; Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832002, Xinjiang, China.
authors.forename: Qiu-sheng
authors.initials: QS
authors.lastname: Zheng
authors.affiliation: The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong, China.
authors.forename: Chang-gui
authors.initials: CG
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002316:Cardiotonic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D006334:Heart Function Tests; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010477:Perfusion; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D047188:Chalcones; C541529:licochalcone D; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D013482:Superoxide Dismutase; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases; D005978:Glutathione