title: Determination of mannitol sorbitol and myo-inositol in olive tree roots and rhizospheric soil by gas chromatography and effect of severe drought conditions on their profiles.
abstract: This study reports a method for the analysis of mannitol, sorbitol and myo-inositol in olive tree roots and rhizospheric soil with gas chromatography. The analytical method consists of extraction with a mixture of dichloromethane:methanol (2:1, v/v) for soil samples and a mixture of ethanol:water (80:20) for root samples, silylation using pyridine, hexamethyldisilazane (HMDS) and trimethylchlorosilane (TMCS). The recovery of mannitol sorbitol and myo-inositol (for extraction and analysis in dichloromethane:methanol and ethanol:water) was acceptable and ranged from 100.3 to 114.7%. The time of analysis was <24 min. Among identified polyols extracted from rhizosphere and roots of olive plants, mannitol was the major compound. A marked increase in mannitol content occurred in rhizosphere and roots of water-stressed plants, suggesting a much broader role of mannitol in stress response based on its ability to act as a compatible solute.
authors.affiliation: Laboratoire de Biochimie, USCR Spectrométrie de Masse, LR-NAFS/LR12ES05 Nutrition-aliments fonctionnels et santé vasculaire, Faculté de Médecine, Université de Monastir, Monastir 5019, Tunisia beligh.mechri77@yahoo.fr.
authors.forename: Beligh
authors.initials: B
authors.lastname: Mechri
authors.affiliation: Laboratoire de Biochimie, USCR Spectrométrie de Masse, LR-NAFS/LR12ES05 Nutrition-aliments fonctionnels et santé vasculaire, Faculté de Médecine, Université de Monastir, Monastir 5019, Tunisia.
authors.forename: Meriem
authors.initials: M
authors.lastname: Tekaya
authors.affiliation: Laboratoire d'Ecophysiologie, Institut de L'Olivier de Sousse, Sousse, Tunisia.
authors.forename: Hechmi
authors.initials: H
authors.lastname: Cheheb
authors.affiliation: Laboratoire de Biochimie, USCR Spectrométrie de Masse, LR-NAFS/LR12ES05 Nutrition-aliments fonctionnels et santé vasculaire, Faculté de Médecine, Université de Monastir, Monastir 5019, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D055864:Droughts; D007294:Inositol / Q000032:analysis*; D008353:Mannitol / Q000032:analysis*; D031658:Olea / Q000737:chemistry* / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry*; D013012:Sorbitol / Q000032:analysis*
chemical_list: D008353:Mannitol; D007294:Inositol; D013012:Sorbitol