title: Chrysophanol affords neuroprotection against microglial activation and free radical-mediated oxidative damage in BV2 murine microglia.
abstract: In this study, chrysophanol, isolated from a marine fungus, was examined for its protective effects against inflammatory responses and oxidative stress in BV2 microglia. Chrysophanol was studied to assess its capabilities of protecting against lipopolysaccharide (LPS)-induced inflammatory responses in BV2 cells. It was found that chrysophanol reduced the level of nitric oxide (NO) and prostaglandin-E2 (PGE2) production by diminishing reducing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Assessment of the inhibitory activities of chrysophanol on the generation of pro-inflammatory cytokines was also performed. Furthermore, Chrysophanol treatment significantly reduced intracellular reactive oxygen species (ROS)-mediated cell damage and inhibited DNA oxidation in BV2 cells. Moreover, antioxidative mechanisms by of chrysophanol were evaluated investigated by measuring the expression levels of antioxidative enzymes such superoxide dismutase (SOD) and glutathione (GSH). Therefore, results suggested that chrysophanol has potential antioxidant and anti-inflammatory activities in microglia and further might be a useful therapeutic agent for the treatment of neurodegenerative diseases.
authors.affiliation: Department of Anesthesiology, Changhai Hospital, Second Military Medical University Shanghai 200433, China ; Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, 200072, China.
authors.forename: Fuqing
authors.initials: F
authors.lastname: Lin
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, 200072, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, 200072, China.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Chen
authors.affiliation: Lixiang Eye Hospital of Soochow University Suzhou, 215000, China.
authors.forename: E
authors.initials: E
authors.lastname: Song
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, 200072, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Sun
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, 200072, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Chen
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, 200072, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Pan
authors.affiliation: Department of Anesthesiology, Changhai Hospital, Second Military Medical University Shanghai 200433, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Deng
keywords: BV2; Chrysophanol; microglial activation; neuroprotection