title: Enrichment and purification process of astragalosides and their anti-human gastric cancer MKN-74 cell proliferation effect.
abstract: BACKGROUND
  Radix astragali mainly contains saponins, polysaccharides, flavonoids, amino acids and other chemical constituents of which total astragalosides have immunomodulatory, anti-viral, hepatoprotective, and gastric mucosa protective effects.
  
  
  OBJECTIVE
  To investigate the process conditions for extraction, purification and enrichment of total astragalosides by macroporous adsorption resin, and to study the inhibitory effect of total astragalosides on growth of human gastric cancer cell line MKN-74.
  
  
  METHODS
  UV spectrophotometry was applied to determine the adsorption and desorption capacity of macroporous adsorption resin on total astragaloside content, MTT assay was used to determine the inhibition of MKN-74 cell growth by total astragalosides.
  
  
  RESULTS
  The dynamic adsorption performance of DA201 adsorption resin was examined, and the dynamic adsorption curve of total astragalosides on DA201 resin column was plotted. Meanwhile, eluent and elution flow rate were investigated, the results showed that the choice of eluent of 80% ethanol, and a flow rate of 5 BV/h could maximize the yield of total astragalosides. MTT assay found that astragalosides could relatively pronouncedly inhibit the proliferation of MKN-74 cells, and the inhibitory effect was enhanced with the increase of astragaloside dose and the extension of processing time, which showed a dose-and time-dependence.
  
  
  CONCLUSIONS
  DA201 resin can effectively enrich total astragalosides, total astragalosides have an inhibitory effect on growth of MKN-74 cells.
authors.affiliation: Department of General Surgery 8W, Xiangya Hospital of Central-South University, Changsha, 410008 China.
authors.forename: Yang
authors.initials: Y
authors.lastname: OuYang
authors.affiliation: Department of General Surgery 8W, Xiangya Hospital of Central-South University, Changsha, 410008 China.
authors.forename: JianHua
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of General Surgery 8W, Xiangya Hospital of Central-South University, Changsha, 410008 China.
authors.forename: ZingTing
authors.initials: Z
authors.lastname: OuYang
authors.affiliation: Department of General Surgery 8W, Xiangya Hospital of Central-South University, Changsha, 410008 China.
authors.forename: Jin
authors.initials: J
authors.lastname: Kang
mesh_terms: D000327:Adsorption; D027883:Astragalus Plant / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D012117:Resins, Synthetic; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A
keywords: DA201 Resin; MKN-74 Cell; Total astragalosides