title: Metabolomic profiles illuminate the efficacy of Chinese herbal Da-Cheng-Qi decoction on acute pancreatitis in rats.
abstract: OBJECTIVE
  Chinese herbal drug Da-Cheng-Qi decoction (DCQD) has been widely used for decades to treat acute pancreatitis (AP). Previous trials are mostly designed to state the potential mechanisms of the therapeutic effects rather than to detect its whole effect on metabolism. This study aimed to investigate the efficacy of DCQD on metabolism in AP.
  
  
  METHODS
  Twenty-two male adult Sprague-Dawley rats were randomized into three groups. AP was induced by retrograde ductal infusion of 3.5% sodium taurocholate solution in DCQD and AP group, while 0.9% saline solution was used in sham operation (SO) group. Blood samples were obtained 12 h after drug administration and a 600 MHz superconducting Nuclear Magnetic Resonance (NMR) spectrometer was used to detected plasma metabolites. Principal Components Analysis (PCA) and Partial Least Squares-Discriminant Analysis after Orthogonal Signal Correction (OSC-PLS-DA) were applied to analyze the Longitudinal Eddy-delay (LED) and Carr-Purcell-Meiboom-Gill (CPMG) spectra.
  
  
  RESULTS
  Differences in concentrations of metabolites among the three groups were detected by OSC-PLS-DA of 1HNMR spectra (both LED and CPMG). Compared with SO group, DCQD group had higher levels of plasma glycerol, glutamic acid, low density lipoprotein (LDL), saturated fatty acid (FA) and lower levels of alanine and glutamine, while the metabolic changes were reversed in the AP group.
  
  
  CONCLUSIONS
  Our results demonstrated that DCQD was capable of altering the changed concentrations of metabolites in rats with AP and 1HNMR-based metabolomic approach provided a new methodological cue for systematically investigating the efficacies and mechanisms of DCQD in treating AP.
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Shi-Feng
authors.initials: SF
authors.lastname: Zhu
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Xian-Lin
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China; China Tibetology Research Center, 100101, Beijing, China.
authors.forename: Yi-Xia
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Wan
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Han-Lin
authors.initials: HL
authors.lastname: Gong
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Chen
authors.affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China. Electronic address: hxtangwenfu@126.com.
authors.forename: Wen-Fu
authors.initials: WF
authors.lastname: Tang
mesh_terms: D000409:Alanine / Q000097:blood; D000818:Animals; D001711:Biotransformation; D008078:Cholesterol, LDL / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D005227:Fatty Acids / Q000097:blood; D018698:Glutamic Acid / Q000097:blood; D005973:Glutamine / Q000097:blood; D005990:Glycerol / Q000097:blood; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D010195:Pancreatitis / Q000188:drug therapy* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008078:Cholesterol, LDL; C551430:Da-Cheng-Qi; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D005973:Glutamine; D018698:Glutamic Acid; D000409:Alanine; D005990:Glycerol
keywords: 1HNMR; Acute pancreatitis; CPMG; Da-Cheng-Qi decoction; Metabolomics; OSC-PLS-DA analysis