title: Two leptin genes and a leptin receptor gene of female chub mackerel (Scomber japonicus): Molecular cloning, tissue distribution and expression in different obesity indices and pubertal stages.
abstract: Leptin is a hormone produced by fat cells that regulates the amount of fat stored in the body and conveys nutritional status to the reproductive axis in mammals. In the present study we identified two subtypes of leptin genes (lepa and lepb) and a leptin receptor gene (lepr) from chub mackerel (Scomber japonicus) and there gene expression under different feeding conditions (control and high-feed) and pubertal development stages was analyzed using quantitative real-time PCR. The protein lengths of LepA, LepB and LepR were 161 amino acids (aa), 163 aa and 1149 aa, respectively and both leptin subtypes shared only 15% similarity in aa sequences. In pubertal females, lepa was expressed in the brain, pituitary gland, liver, adipose tissue and ovary; however, in adult (gonadal maturation after the second in the life) females, lepa was expressed only in the liver. lepb was expressed primarily in the brain of all fish tested and was expressed strongly in the adipose tissue of adults. lepr was characterized by expression in the pituitary. The high-feed group showed a high conditioning factor level; unexpectedly, hepatic lepa and brain lepr were significantly more weakly expressed compared with the control-feed group. Furthermore, the expression levels of lepa, lepb and lepr genes showed no significant differences between pre-pubertal and post-pubertal fish. On the other hand, pituitary fshβ and lhβ showed no significant differences between different feeding groups of pre-pubertal fish. In contrast, fshβ and lhβ expressed abundantly in the post-pubertal fish of control feed group. Based on these results, whether leptin plays an important role in the nutritional status and pubertal onset of chub mackerel remains unknown.
authors.affiliation: Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ohga
authors.affiliation: Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Kojiro
authors.initials: K
authors.lastname: Matsumori
authors.affiliation: Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kodama
authors.affiliation: Fisheries Research Institute of Karatsu, Kyushu University, Saga 847-0132, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kitano
authors.affiliation: Fisheries Research Institute of Karatsu, Kyushu University, Saga 847-0132, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagano
authors.affiliation: Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yamaguchi
authors.affiliation: Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan. Electronic address: rinya_m@agr.kyushu-u.ac.jp.
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsuyama
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000378:metabolism*; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006801:Humans; D020738:Leptin / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D054411:Receptors, Leptin / Q000235:genetics*; D012098:Reproduction / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D020738:Leptin; D054411:Receptors, Leptin
keywords: Energy metabolism; Leptin; Leptin receptor; Puberty; Scombridae