title: Enzymatic Decoration of Prebiotic Galacto-oligosaccharides (Vivinal GOS) with Sialic Acid Using Trypanosoma cruzi trans-Sialidase and Two Bovine Sialoglycoconjugates as Donor Substrates.
abstract: Decoration of prebiotic galacto-oligosaccharides (GOS) with sialic acid yields mixtures of GOS and sialylated GOS (Sia-GOS), novel products that are expected to have both prebiotic and antiadhesive functionalities. The recombinantly produced trans-sialidase enzyme from Trypanosoma cruzi (TcTS), an enzyme with the ability to transfer (α2-3)-linked sialic acid from sialogalactoglycans to asialogalactoglycans, was employed to catalyze this sialylation. As sialic acid acceptor substrates, Vivinal GOS and derived fractions of specific degree of polymerization were taken. As sialic acid donor substrates, bovine κ-casein-derived glycomacropeptide [>99% N-acetylneuraminic acid (Neu5Ac); <1% N-glycolylneuraminic acid (Neu5Gc)] and bovine blood plasma glycoprotein mixture (45% Neu5Ac; 55% Neu5Gc) were selected, yielding potential food and feed products, respectively. High-pH anion-exchange chromatography, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy were used for product analysis.
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Maarten H
authors.initials: MH
authors.lastname: Wilbrink
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Geert A
authors.initials: GA
authors.lastname: ten Kate
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Peter
authors.initials: P
authors.lastname: Sanders
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.|‡NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Padualaan 8, NL-3584 CH Utrecht, The Netherlands.
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Gerwig
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Sander S
authors.initials: SS
authors.lastname: van Leeuwen
authors.affiliation: §FrieslandCampina Research, Stationsplein 4, NL-3818 LE Amersfoort, The Netherlands.
authors.forename: Erik
authors.initials: E
authors.lastname: Sallomons
authors.affiliation: §FrieslandCampina Research, Stationsplein 4, NL-3818 LE Amersfoort, The Netherlands.
authors.forename: Bert
authors.initials: B
authors.lastname: Klarenbeek
authors.affiliation: #Darling Ingredients Nederland BV, Kanaaldijk Noord 20-21, NL-5691 NM Son, The Netherlands.
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Hage
authors.affiliation: #Darling Ingredients Nederland BV, Kanaaldijk Noord 20-21, NL-5691 NM Son, The Netherlands.
authors.forename: Carine A
authors.initials: CA
authors.lastname: van Vuure
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
authors.affiliation: †Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.|‡NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Padualaan 8, NL-3584 CH Utrecht, The Netherlands.
authors.forename: Johannis P
authors.initials: JP
authors.lastname: Kamerling
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D003116:Color; D006001:Glycoconjugates / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015394:Molecular Structure; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D009439:Neuraminidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*; D056692:Prebiotics / Q000032:analysis*; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000201:enzymology
chemical_list: D002364:Caseins; D006001:Glycoconjugates; D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D056692:Prebiotics; D015800:Protozoan Proteins; C020251:kappa-casein glycomacropeptide; C069715:trans-sialidase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid
keywords: Trypanosoma cruzi; bovine blood plasma glycoprotein; bovine glycomacropeptide; galacto-oligosaccharides; sialic acid; trans-sialidase