title: Effect of Fractionated Irradiation on the Hippocampus in an Experimental Model.
abstract: BACKGROUND
  Ionizing radiation induces altered brain tissue homeostasis and can lead to morphological and functional deficits. The aim of the present study was to investigate the short-term and long-term effect of ionizing radiation on cell population resides adult rat hippocampus.
  
  
  METHODS
  Adult male Wistar rats received whole- brain irradiation with fractionated doses of gamma rays (a total dose of 20 Gy) and were investigated 30 and 100 days later. A combination of Fluoro-Jade C histochemistry for visualization of degenerating neurons, immunohistochemistry for detection of astrocytes and confocal microscopy were used to quantify the neurodegenerative changes in the hippocampal dentate gyrus and CA1 subfield.
  
  
  RESULTS
  A significant increase of Fluoro-Jade C labelled neurons was seen in both of investigated areas through the whole experiment, predominantly 30 days after irradiation. Non- significant decrease of GFAP- immunoreactive astrocytes was found in the hippocampal dentate gyrus and CA1 subfield until 100 days after irradiation.
  
  
  CONCLUSIONS
  Our recent results showed that radiation response of cell types resides the adult hippocampus may play contributory role in the development of adverse radiation-induced late effects.
authors.forename: S
authors.initials: S
authors.lastname: Bálentová
authors.forename: E
authors.initials: E
authors.lastname: Hajtmanová
authors.forename: B
authors.initials: B
authors.lastname: Filova
authors.forename: V
authors.initials: V
authors.lastname: Borbelyova
authors.forename: J
authors.initials: J
authors.lastname: Lehotský
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D019583:Dose Fractionation, Radiation; D005720:Gamma Rays; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D007150:Immunohistochemistry; D008297:Male; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005904:Glial Fibrillary Acidic Protein