title: In vitro Potential Effect of Morin in the Combination with β-Lactam Antibiotics Against Methicillin-Resistant Staphylococcus aureus.
abstract: Morin, a plant-derived flavonol, is known to be an effective inhibitor of Gram-positive bacteria. In this study, we explored the combined effect of morin with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA), a multidrug-resistant pathogen. The anti-MRSA activity of morin was investigated by the broth microdilution method, checkerboard dilution test, and time-kill curve assay. The expression of the resistant protein, penicillin-binding protein (PBP2a) encoded by mecA, was analyzed by the Western blotting method in the presence of morin and oxacillin. An increased susceptibility of MRSA toward oxacillin was observed in the presence of morin. The protein level of PBP2a was reduced when MRSA (ATCC 33591) was treated with the combination of morin and oxacillin, indicating that the combination of morin and oxacillin potentiates the killing effect against MRSA. The present study indicates that the killing effect by the combinative treatment of morin and β-lactam antibiotic is dependent on the PBP2a-mediated resistance mechanism.
authors.affiliation: 1 BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Mun
authors.affiliation: 2 Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science , RDA, Eumsung, Chungbuk, Korea.
authors.forename: Young-Seob
authors.initials: YS
authors.lastname: Lee
authors.affiliation: 2 Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science , RDA, Eumsung, Chungbuk, Korea.
authors.forename: Sin-Hee
authors.initials: SH
authors.lastname: Han
authors.affiliation: 2 Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science , RDA, Eumsung, Chungbuk, Korea.
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.affiliation: 2 Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science , RDA, Eumsung, Chungbuk, Korea.
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Cha
authors.affiliation: 1 BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Sung-bae
authors.initials: SB
authors.lastname: Kim
authors.affiliation: 3 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Yun-Soo
authors.initials: YS
authors.lastname: Seo
authors.affiliation: 1 BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Ryong
authors.initials: R
authors.lastname: Kong
authors.affiliation: 3 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Da-Hye
authors.initials: DH
authors.lastname: Kang
authors.affiliation: 4 Department of Oriental Medicine Resources, College of Bio Industry Science, Sunchon National University , Sunchon, Jeonnam, Korea.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Shin
authors.affiliation: 3 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Ok-Hwa
authors.initials: OH
authors.lastname: Kang
authors.affiliation: 1 BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University , Iksan, Jeonbuk, Korea.|3 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University , Iksan, Jeonbuk, Korea.
authors.forename: Dong-Yeul
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D000667:Ampicillin / Q000819:agonists / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002473:Cell Wall / Q000187:drug effects / Q000648:ultrastructure; D015169:Colony Count, Microbial; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D004357:Drug Synergism; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D010068:Oxacillin / Q000819:agonists / Q000494:pharmacology; D046915:Penicillin-Binding Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D056910:Republic of Korea; D013203:Staphylococcal Infections / Q000382:microbiology; D047090:beta-Lactams / Q000819:agonists* / Q000494:pharmacology; D047309:Flavones
chemical_list: D000667:Ampicillin; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005419:Flavonoids; D010068:Oxacillin; D046915:Penicillin-Binding Proteins; D047090:beta-Lactams; C487789:mecA protein, Staphylococcus aureus; C008548:morin; D047309:Flavones