title: Human embryonic stem cell-derived pancreatic endoderm alleviates diabetic pathology and improves reproductive outcome in C57BL/KsJ-Lep(db/+) gestational diabetes mellitus mice.
abstract: Gestational diabetes mellitus is a condition commonly encountered during mid to late pregnancy with pathologic manifestations including hyperglycemia, hyperinsulinemia, insulin resistance, and fetal maldevelopment. The cause of gestational diabetes mellitus can be attributed to both genetic and environmental factors, hence complicating its diagnosis and treatment. Pancreatic progenitors derived from human embryonic stem cells were shown to be able to effectively treat diabetes in mice. In this study, we have developed a system of treating diabetes using human embryonic stem cell-derived pancreatic endoderm in a mouse model of gestational diabetes mellitus. Human embryonic stem cells were differentiated in vitro into pancreatic endoderm, which were then transplanted into db/+ mice suffering from gestational diabetes mellitus. The transplant greatly improved glucose metabolism and reproductive outcome of the females compared with the control groups. Our findings support the feasibility of using differentiated human embryonic stem cells for treating gestational diabetes mellitus patients.
authors.affiliation: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou 061001, People's Republic of People's Republic of China.
authors.forename: Baoheng
authors.initials: B
authors.lastname: Xing
authors.affiliation: Department of Neurology, Cangzhou City People's Hospital, Cangzhou 061000, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou 061001, People's Republic of People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou 061001, People's Republic of People's Republic of China.
authors.forename: Yalei
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Obstetrics, Cangzhou City Central Hospital, Cangzhou 061001, People's Republic of People's Republic of China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Gynecology and Obstetrics, Bethune International Peace Hospital, Shijiazhuang 050051, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Gynecology and Obstetrics, Bethune International Peace Hospital, Shijiazhuang 050051, People's Republic of China. Electronic address: xhwu12@163.com.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002454:Cell Differentiation; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000628:therapy*; D016640:Diabetes, Gestational / Q000097:blood / Q000628:therapy*; D004707:Endoderm; D005260:Female; D047109:Fetal Development; D000066449:Human Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D010179:Pancreas; D011247:Pregnancy; D011256:Pregnancy Outcome; D016378:Tissue Transplantation
chemical_list: D001786:Blood Glucose
keywords: C57BL/KsJ-Lep(db/+) mouse model; Gestational diabetes mellitus; Human embryonic stem cell–derived pancreatic endoderm; Hyperglycemia; Hyperinsulinemia; Stem cell therapy