title: iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes.
abstract: Non-starch polysaccharide enzymes (NSPEs) have long been used in the feed production of monogastric animals to degrade non-starch polysaccharide to oligosaccharides and promote growth performance. However, few studies have been conducted on the effect of such enzymes on skeletal muscle in monogastric animals. To elucidate the mechanism of the effect of NSPEs on skeletal muscle, an isobaric tag for relative and absolute quantification (iTRAQ) for differential proteomic quantitation was applied to investigate alterations in the proteome in the longissimus muscle (LM) of growing pigs after a 50-d period of supplementation with 0.6% NSPEs in the diet. A total of 51 proteins were found to be differentially expressed in the LM between a control group and the NSPE group. Functional analysis of the differentially expressed protein species showed an increased abundance of proteins related to energy production, protein synthesis, muscular differentiation, immunity, oxidation resistance and detoxification, and a decreased abundance of proteins related to inflammation in the LM of the pigs fed NSPEs. These findings have important implications for understanding the mechanisms whereby dietary supplementation with NSPEs enzymes can promote growth performance and improve muscular metabolism in growing pigs.
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Animal Science and Technology, Jilin University, Changchun 130062, China.
authors.forename: Ji-ze
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Qing-ping
authors.initials: QP
authors.lastname: Lu
authors.forename: Ren-na
authors.initials: RN
authors.lastname: Sa
authors.forename: Hong-fu
authors.initials: HF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004798:Enzymes / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D064170:Paraspinal Muscles / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D013552:Swine / Q000502:physiology*
chemical_list: D004798:Enzymes; D009124:Muscle Proteins; D020543:Proteome
keywords: Growing pigs; Longissimus muscle; Non-starch polysaccharide enzymes (NSPEs); Proteomics