title: TSPAN1 functions as an oncogene in gastric cancer and is downregulated by miR-573.
abstract: Tetraspanin 1 (TSPAN1) has been reported to be upregulated in gastric cancer (GC). However, whilst TSPAN1 is positively correlated with clinical stage and negatively correlated with survival rates, its function in GC remains elusive. Here we show that expression of TSPAN1 is significantly higher in GC tissues compared to non-cancerous tissues. Furthermore, we demonstrate that RNAi-mediated down-regulation of TSPAN1 expression markedly blocks GC cell proliferation, cell cycle progression and invasive activity. We identified TSPAN1 as a novel target gene of miR-573. Overexpression of miR-573 suppressed proliferation and invasion of GC cells by down-regulation of TSPAN1 expression. Restoration of TSPAN1 rescued the effects of miR-573 overexpression. Therefore, our findings suggest that the miR-573/TSPAN1 axis is important in the control of gastric carcinogenesis.
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhengmao
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Tianhang
authors.initials: T
authors.lastname: Luo
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Nie
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Pang
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Liye
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Bi
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Guoen
authors.initials: G
authors.lastname: Fang
authors.affiliation: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China. Electronic address: xuexch1@163.com.
authors.forename: Xuchao
authors.initials: X
authors.lastname: Xue
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D017931:DNA Primers; D015536:Down-Regulation / Q000502:physiology*; D005260:Female; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009857:Oncogenes; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D059470:Tetraspanins / Q000235:genetics / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; C585063:MIRN573 microRNA, human; D035683:MicroRNAs; C508125:TSPAN1 protein, human; D059470:Tetraspanins
keywords: Gastric cancer; Tetraspanin 1; miR-573