title: Comparison of the effect of raw and blanched-frozen broccoli on DNA damage in colonocytes.
abstract: Consumption of cruciferous vegetables may protect against colorectal cancer. Cruciferous vegetables are rich in a number of bioactive constituents including polyphenols, vitamins and glucosinolates. Before consumption, cruciferous vegetables often undergo some form of processing that reduces their content of bioactive constituents and may determine whether they exert protective effects. The aim of this study was to compare the ability of raw and blanched-frozen broccoli to protect colonocytes against DNA damage, improve antioxidant status and induce xenobiotic metabolizing enzymes (XME). Fifteen Landrace × Large White male pigs were divided into five age-matched and weight-matched sets (79 days, SD 3, and 34·7 kg, SD 3·9, respectively). Each set consisted of siblings to minimize genetic variation. Within each set, pigs received a cereal-based diet, unsupplemented (control) or supplemented with 600 g day(-1) of raw or blanched-frozen broccoli for 12 days. The consumption of raw broccoli caused a significant 27% increase in DNA damage in colonocytes (p = 0·03) relative to the control diet, whereas blanched-frozen broccoli had no significant effect. Both broccoli diets had no significant effect on plasma antioxidant status or hepatic and colonic XME. This study is the first to report that the consumption of raw broccoli can damage DNA in porcine colonocytes.
authors.affiliation: Food Group, Sheffield Hallam University, Sheffield, UK.
authors.forename: Anthony
authors.initials: A
authors.lastname: Lynn
authors.affiliation: School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, UK.
authors.forename: Zoë
authors.initials: Z
authors.lastname: Fuller
authors.affiliation: Department of Nutrition, University of Oslo, Oslo, Norway.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Collins
authors.affiliation: School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, UK.
authors.forename: Brian
authors.initials: B
authors.lastname: Ratcliffe
mesh_terms: D000818:Animals; D001937:Brassica / Q000009:adverse effects* / Q000201:enzymology; D003106:Colon / Q000166:cytology* / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D005628:Frozen Foods / Q000009:adverse effects*; D006026:Glycoside Hydrolases / Q000378:metabolism; D008297:Male; D066256:Raw Foods / Q000009:adverse effects*; D013552:Swine; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D015262:Xenobiotics; D006026:Glycoside Hydrolases; C022739:thioglucosidase
keywords: DNA damage; broccoli; comet assay; glucosinolates