title: Effects of Arsenic Trioxide Exposure on Heat Shock Protein Response in the Immune Organs of Chickens.
abstract: Arsenic trioxide (As2O3), a kind of pentavalent arsenic, has recently been linked to disrupted immune function. Heat shock proteins (Hsps), a group of highly conserved proteins, are rapidly synthesised when living organisms are exposed to various stress conditions. The objective of this study is to determine the effects of As2O3 on the expression level of Hsps (Hsp90, Hsp70, Hsp60, Hsp40 and Hsp27) in the immune organs (spleen, thymus and bursa of Fabricius (BF)) of chickens. A total of 72 1-day-old male Hy-line chickens were randomly divided into four groups, including the low-As group (L group), middle-As group (M group), high-As group (H group) and control group (C group). Immune organs were collected, and levels of Hsp messenger RNA (mRNA) and protein were examined on days 30, 60 and 90. The results showed that the levels of Hsp mRNA (Hsp90, Hsp70, Hsp60, Hsp40 and Hsp27) and protein (Hsp70 and Hsp60) expression were significantly increased (p < 0.05 or p < 0.01) in the As2O3 treatment groups compared with the corresponding control groups. Taken together, these results suggest that As2O3 influences the level of Hsps in immune organs.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China. guoying0601@sina.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China. zpp13796033057@163.com.
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China.
authors.forename: Guangyang
authors.initials: G
authors.lastname: Guo
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Hu
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Heilongjiang Zhalong Natural Protection Area Management Bureau, Qiqihar, 161000, Heilongjiang Province, China. ZLzhangxianguang@163.com.
authors.forename: Xianguang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China. zhangwenwakeup@126.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China. xingmingweinefu@sina.com.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Xing
mesh_terms: D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002645:Chickens; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects*; D008297:Male; D010087:Oxides / Q000494:pharmacology*
chemical_list: D001152:Arsenicals; D055551:HSP27 Heat-Shock Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D010087:Oxides; D000077237:Arsenic Trioxide
keywords: Arsenic trioxide; Chicken; Heat shock protein; Immune organs