title: Isolation and purification of prenylated phenolics from Amorpha fruticosa by high-speed counter-current chromatography.
abstract: Prenylated phenolics such as amorfrutins are recently identified potent anti-inflammatory and antidiabetic natural products. In this work, high-speed counter-current chromatography was investigated for the isolation and purification of prenylated phenolics from the fruits of Amorpha fruticosa by using a two-phase solvent system composed of n-hexane/ethanol/water (5:4:1, v/v). As a result, 14.2 mg of 5,7-dihydroxy-8-geranylflavanone, 10.7 mg of amorfrutin A and 17.4 mg of amorfrutin B were obtained from 200 mg of n-hexane-soluble crude extract in one step within 250 min. The purities of 5,7-dihydroxy-8-geranylflavanone, amorfrutins A and B were 95.2, 96.7 and 97.1%, respectively, as determined by ultra high performance liquid chromatography. The structural identification was performed by mass spectrometry and (1) H and (13) C NMR spectroscopy. The results indicated that the established method is an efficient and convenient way to purified prenylated phenolics from A. fruticosa extract.
authors.affiliation: Sichuan Academy of Chinese Medicine Sciences, Chengdu, China.
authors.forename: Chu
authors.initials: C
authors.lastname: Chen
authors.affiliation: Sichuan Academy of Chinese Medicine Sciences, Chengdu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Sciences, Zunyi Medical University Zhuhai Campus, Zhuhai, Guangdong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Du
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D010636:Phenols; D010936:Plant Extracts
keywords: Amorfrutin; Amorpha fruticosa; High-speed counter-current chromatography; Prenylated phenolics