title: Associations of deregulation of mir-365 and its target mRNA TTF-1 and survival in patients with NSCLC.
abstract: microRNA (mir)-365 exerts tumor suppressor function by targeting thyroid transcription factor-1 (TTF-1) in lung cancer cells. The purpose of the present study was to assess mir-365 and its target mRNA TTF-1 in lung cancer and their correlations with patients' survival. Quantitative real-time PCR was used to examine the expression levels of mir-365 and TTF-1 in tumor tissue and its adjacent noncancerous tissue of 126 patients with non-small cell lung cancer (NSCLC). Our results showed that mir-365 was significantly decreased in tumor tissue than that in normal tissue (P=0.006), however, TTF-1 was significantly increased in tumor tissue than in normal tissue (P<0.001). Besides, significant correlations between decreased mir-365 and advanced tumor-node-metastasis (TNM) stage (P=0.001) and regional lymph node involvement (P=0.037) was observed. The similar result was also found between increased TTF-1 and TNM stage (P=0.003). Furthermore, mir-365 downregulation or TTF-1 upregulation were associated with poor outcome of patients than mir-365 upregulation or TTF-1 downregulation (for mir-365: P<0.001; for TTF-1: P=0.002). Of note, combination of decreased mir-365 and increased TTF-1 had worst overall survival (P<0.001). In conclusion, aberrant expression of mir-365/TTF-1 may be involved in the tumor development in patients with NSCLC. Moreover, mir-365 and TTF-1 could jointly predict the prognosis of patients and their combination may serve as a biomarker to predict risk of poor survival in NSCLC patients. Mir-365/TTF-1 might serve as a potential therapeutic target for clinical treatment of NSCLC.
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center 76 Yanta West Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, Tumor Hospital of Shaanxi Province 309 Yanta West Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Oncology Surgery, The First Affiliated Hospital of Xi'an Jiaotong University 277 Yanta West Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Thoracic Surgery, Tumor Hospital of Shaanxi Province 309 Yanta West Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Lv
authors.affiliation: Department of General Thoracic Surgery, 521 Ordnance Hospital 12 Zhangba East Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, Tumor Hospital of Shaanxi Province 309 Yanta West Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Guangyan
authors.initials: G
authors.lastname: Lei
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center 76 Yanta West Road, Xi'an, Shaanxi, P. R. China.
authors.forename: Changfu
authors.initials: C
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000556:secondary; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C546819:MIRN365 microRNA, human; D035683:MicroRNAs; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors
keywords: NKX2-1; microRNA-365; non-small cell lung cancer; outcome; prognostic