title: Therapeutic effects of human urocortin-1, -2 and -3 in intracerebral hemorrhage of rats.
abstract: Urocortin exerts neuroprotective effects in intracerebral hemorrhage (ICH) of rats. For pre-clinical trial, we intended to study the neuroprotective efficacy of human UCN (hUCN)-1, -2 and -3 in treating ICH rats. ICH was induced by infusing bacterial collagenase VII (0.23 U in sterile saline) to the striatum. The hUCN-1, -2, and -3 were administrated (2.5μg/kg, i.p.) at 1h after ICH insult, respectively. Neurological deficits were evaluated by modified Neurological Severity Scores. Brain edema and hematoma expansion was evaluated by coronal T2-WI and DWI magnetic resonance imaging on 1, 3, 6, 24, and 56h after ICH insult. Blood-brain barrier permeability was evaluated by Evans blue assay on day 3 after ICH. Brain lesion volume was evaluated by morphormetric measurement on day 7 after ICH. Our results demonstrated that the hUCN-1 significantly reduced hematoma, blood-brain barrier disruption and neurological deficits on day 3, and brain lesion volume on day 7 after ICH insult. The prediction of secondary structure of the hUCNs clarifies that the percentage of alpha-helix, random coil and extended strand between rat-UCN (rUCN)-1 and hUCN-1 are the same. The structure similarity between human- and rat-UCN-1 may be one of the reasons that both can exert similar therapeutic potential in ICH rats.
authors.affiliation: Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC.
authors.forename: Hock-Kean
authors.initials: HK
authors.lastname: Liew
authors.affiliation: Department of Radiology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC; Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan, ROC.
authors.forename: Li-Chuan
authors.initials: LC
authors.lastname: Huang
authors.affiliation: Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC.
authors.forename: Hui-I
authors.initials: HI
authors.lastname: Yang
authors.affiliation: Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC.
authors.forename: Hsiao-Fen
authors.initials: HF
authors.lastname: Peng
authors.affiliation: Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC; Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC.
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Li
authors.affiliation: Institute of Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan, ROC.
authors.forename: Andy Po-Yi
authors.initials: AP
authors.lastname: Tsai
authors.affiliation: Department of Neurosurgery, Buddhist Tzu Chi General Hospital, Tzu Chi University, Hualien, Taiwan, ROC; Neuro-Medical Scientific Center, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC.
authors.forename: Shin-Yuan
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Institute of Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan, ROC; Neuro-Medical Scientific Center, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC.
authors.forename: Jon-Son
authors.initials: JS
authors.lastname: Kuo
authors.affiliation: Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC; Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan, ROC. Electronic address: cypang@mail.tcu.edu.tw.
authors.forename: Cheng-Yoong
authors.initials: CY
authors.lastname: Pang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000517:prevention & control*; D003342:Corpus Striatum / Q000187:drug effects* / Q000473:pathology*; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D003012:Microbial Collagenase; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D054832:Urocortins / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D018696:Neuroprotective Agents; C576814:UCN protein, human; C426705:UCN2 protein, human; C426704:UCN3 protein, human; D054832:Urocortins; D003346:Corticotropin-Releasing Hormone; C533954:collagenase VII-S, Clostridium histolyticum; D003012:Microbial Collagenase
keywords: Brain edema; Brain lesion; Intracerebral hemorrhage (ICH); Neuroprotection; Urocortins