title: Three-dimensional migration of neutrophils through an electrospun nanofibrous membrane.
abstract: The study of immune cell migration is important for understanding the immune system network, which is associated with the response to foreign cells. Neutrophils act against foreign cells before any other immune cell, and they must be able to change shape and squeeze through narrow spaces in the extracellular matrix (ECM) during migration to sites of infection. Conventional in vitro migration assays are typically performed on two-dimensional substrates that fail to reproduce the three-dimensional (3-D) nature of the ECM. Here we present an in vitro method to simulate the 3-D migration of neutrophils using an electrospun nanofibrous membrane, which is similar to the ECM in terms of morphology. We examined the properties of neutrophil movement and the effects of gravity and the presence of IL-8, which has been widely used as a chemotactic attractant for neutrophils. The number of neutrophils passing through the nanofibrous membrane were higher, and their movement was more active in the presence of IL-8. Also, we confirmed that neutrophils could migrate against gravity toward IL-8 through a nanofibrous membrane.
authors.affiliation: Department of Mechanical Engineering, Korea Polytechnic University, Siheung, Gyeonggy-do, South Korea.
authors.forename: Songwan
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Advanced Convergence Technology, Korea Polytechnic University, Siheung, Gyeonggy-do, South Korea.
authors.forename: Tae-Min
authors.initials: TM
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Dong-A University, Busan, South Korea.
authors.forename: Cho-Hee
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Korea Polytechnic University, Siheung, Gyeonggy-do, South Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Korea Polytechnic University, Siheung, Gyeonggy-do, South Korea.
authors.forename: Binh Duong
authors.initials: BD
authors.lastname: Le
authors.affiliation: School of Mechanical Engineering, Kyungpook National University, Daegu, South Korea.
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Jeong
authors.affiliation: Department of Pharmacology, Ajou University School of Medicine, Suwon, Gyeonggy-do, South Korea.
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, South Korea.
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
mesh_terms: D000818:Animals; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D002630:Chemotactic Factors / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D005109:Extracellular Matrix / Q000737:chemistry; D006112:Gravitation; D016209:Interleukin-8 / Q000276:immunology; D008567:Membranes, Artificial; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology
chemical_list: D002630:Chemotactic Factors; D016209:Interleukin-8; D008567:Membranes, Artificial
keywords: 3-D cell migration; electrospinning; extracellular matrix; nanofibrous membrane; neutrophil