title: Preventive Effect of Lactobacillus fermentum Zhao on Activated Carbon-Induced Constipation in Mice.
abstract: The aim of this study was to investigate the effects of Lactobacillus fermentum Zhao (LF-Zhao) on activated carbon-induced constipation in ICR mice. ICR mice were administered lactic acid bacteria by gavage for 9 d. Body weight, diet intake, drinking amount, stool status, gastrointestinal transit distance and stool time, in addition to motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) levels in serum were monitored to evaluate the preventive effects of LF-Zhao on constipation. Bisacodyl, a laxative drug, was used as a positive control. Times to the first black stool for normal (untreated), control (no lactic acid bacteria treatment but activated carbon treated), bisacodyl-treated and L. delbrueckii subsp. bulgaricus (LB), LF-Zhao (L) (low concentration of 1×10(8) CFU/mL)- and LF-Zhao (H) (high concentration of 1×10(9) CFU/mL)-treated mice induced by activated carbon were 90, 218, 117, 180, 169 and 156 min, respectively. Following the consumption of LB, LF-Zhao (L) and LF-Zhao (H) or the oral administration of bisacodyl, the gastrointestinal transit distances were reduced by 55.2%, 61.3%, 70.6% and 94.6%, respectively. The serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Zhao compared with those in the control mice (p<0.05). These results demonstrated that lactic acid bacteria demonstrate preventive effects on mouse constipation and that LF-Zhao alleviated constipation symptoms better than LB.
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Qian
authors.forename: Huayi
authors.initials: H
authors.lastname: Suo
authors.forename: Muying
authors.initials: M
authors.lastname: Du
authors.forename: Guijie
authors.initials: G
authors.lastname: Li
authors.forename: Zhenhu
authors.initials: Z
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Li
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D001835:Body Weight; D002244:Carbon; D003248:Constipation / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D003672:Defecation; D016232:Endothelins / Q000097:blood; D005243:Feces; D005260:Female; D005755:Gastrins / Q000097:blood; D005772:Gastrointestinal Transit; D052197:Limosilactobacillus fermentum; D054368:Laxatives / Q000627:therapeutic use*; D008813:Mice, Inbred ICR; D009037:Motilin / Q000097:blood; D013004:Somatostatin / Q000097:blood; D013373:Substance P / Q000097:blood; D014660:Vasoactive Intestinal Peptide / Q000097:blood
chemical_list: D016232:Endothelins; D005755:Gastrins; D054368:Laxatives; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D009037:Motilin; D002244:Carbon; D000110:Acetylcholinesterase