title: Renalase does not catalyze the oxidation of catecholamines.
abstract: It is widely accepted that the function of human renalase is to oxidize catecholamines in blood. However, this belief is based on experiments that did not account for slow, facile catecholamine autoxidation reactions. Recent evidence has shown that renalase has substrates with which it reacts rapidly. The reaction catalyzed defines renalase as an oxidase, one that harvests two electrons from either 2-dihydroNAD(P) or 6-dihydroNAD(P) to form β-NAD(P)(+) and hydrogen peroxide. The apparent metabolic purpose of such a reaction is to avoid inhibition of primary dehydrogenase enzymes by these β-NAD(P)H isomers. This article demonstrates that renalase does not catalyze the oxidation of neurotransmitter catecholamines. Using high-performance liquid chromatography we show that there is no evidence of consumption of epinephrine by renalase. Using time-dependent spectrophotometry we show that the renalase FAD cofactor spectrum is unresponsive to added catecholamines, that adrenochromes are not observed to accumulate in the presence of renalase and that the kinetics of single turnover reactions with 6-dihydroNAD are unaltered by the addition of catecholamines. Lastly we show using an oxygen electrode assay that plasma renalase activity is below the level of detection and only when exogenous renalase and 6-dihydroNAD are added can dioxygen be observed to be consumed.
authors.affiliation: Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St, Milwaukee, WI 53211-3209, United States.
authors.forename: Brett A
authors.initials: BA
authors.lastname: Beaupre
authors.affiliation: Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St, Milwaukee, WI 53211-3209, United States.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Hoag
authors.affiliation: Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, 3210 N. Cramer St, Milwaukee, WI 53211-3209, United States. Electronic address: moran@uwm.edu.
authors.forename: Graham R
authors.initials: GR
authors.lastname: Moran
mesh_terms: D002384:Catalysis; D002395:Catecholamines / Q000737:chemistry*; D004789:Enzyme Activation; D006801:Humans; D008956:Models, Chemical; D008995:Monoamine Oxidase / Q000737:chemistry*; D009249:NADP / Q000737:chemistry*; D018377:Neurotransmitter Agents / Q000737:chemistry*; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D002395:Catecholamines; D018377:Neurotransmitter Agents; D009249:NADP; D008995:Monoamine Oxidase; C521557:renalase
keywords: Catecholamines; Dioxygen; FAD; NAD; Oxidase; Renalase