title: Retinal Angiogenesis Effects of TGF-β1 and Paracrine Factors Secreted From Human Placental Stem Cells in Response to a Pathological Environment.
abstract: Abnormal angiogenesis is a primary cause of many eye diseases, including diabetic retinopathy, age-related macular degeneration, and retinopathy of prematurity. Mesenchymal stem cells (MSCs) are currently being investigated as a treatment for several such retinal diseases based on their neuroprotective and angiogenic potentials. In this study, we evaluated the role of systemically injected human placental amniotic membrane-derived MSCs (AMSCs) on pathological neovascularization of proliferative retinopathy. We determined that AMSCs secrete higher levels of transforming growth factor-β (TGF-β1) than other MSCs, and the secreted TGF-β1 directly suppresses the proliferation of endothelial cells under pathological conditions in vitro. Moreover, in a mouse model of oxygen-induced retinopathy, intraperitoneally injected AMSCs migrated into the retina and suppressed excessive neovascularization of the vasculature via expression of TGF-β1, and the antineovascular effect of AMSCs was blocked by treatment with TGF-β1 siRNA. These findings are the first to demonstrate that TGF-β1 secreted from AMSCs is one of the key factors to suppress retinal neovascularization in proliferative retinopathy and further elucidate the therapeutic function of AMSCs for the treatment of retinal neovascular diseases.
authors.affiliation: College of Life Science, Department of Biotechnology, CHA University, Seoul, Korea.
authors.forename: Kyung-Sul
authors.initials: KS
authors.lastname: Kim
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.forename: TaeHo
authors.initials: T
authors.lastname: Kong
authors.forename: Chul
authors.initials: C
authors.lastname: Kim
authors.forename: Sang-Hun
authors.initials: SH
authors.lastname: Bae
authors.forename: Han Wool
authors.initials: HW
authors.lastname: Kim
authors.forename: Jisook
authors.initials: J
authors.lastname: Moon
mesh_terms: D000650:Amnion / Q000166:cytology; D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D003930:Diabetic Retinopathy / Q000473:pathology; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D007274:Injections, Intraperitoneal; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008810:Mice, Inbred C57BL; D019899:Paracrine Communication; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D015861:Retinal Neovascularization / Q000473:pathology / Q000628:therapy*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1