title: Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method.
abstract: Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid-liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.
authors.affiliation: CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal; Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno s/n, E-37007 Salamanca, Spain.
authors.forename: Lillian
authors.initials: L
authors.lastname: Barros
authors.affiliation: CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Correia
authors.affiliation: CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal. Electronic address: iferreira@ipb.pt.
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
authors.affiliation: CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Baptista
authors.affiliation: Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno s/n, E-37007 Salamanca, Spain.
authors.forename: Celestino
authors.initials: C
authors.lastname: Santos-Buelga
keywords: Analysis; HPLC; Mushrooms; Vitamin E