title: Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia.
abstract: Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets.
authors.affiliation: Universidade Estadual de Maringá-UEM-Maringá, Paraná, 87020-900, Brazil.
authors.forename: Elenice S R
authors.initials: ES
authors.lastname: Goes
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária-EMBRAPA Pantanal-Corumbá, Mato Grosso do Sul, Brazil.
authors.forename: Jorge A F
authors.initials: JA
authors.lastname: Lara
authors.affiliation: Department of Animal Science, Universidade Estadual de Maringá-UEM-Maringá, Paraná, Brazil.
authors.forename: Eliane
authors.initials: E
authors.lastname: Gasparino
authors.affiliation: Department of Animal Science, Universidade Estadual de Maringá-UEM-Maringá, Paraná, Brazil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Del Vesco
authors.affiliation: Universidade Federal do Paraná-UFPR-Palotina, Paraná, Brazil.
authors.forename: Marcio D
authors.initials: MD
authors.lastname: Goes
authors.affiliation: Department of Animal Science, Universidade Estadual de Maringá-UEM-Maringá, Paraná, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Alexandre Filho
authors.affiliation: Department of Animal Science, Universidade Estadual de Maringá-UEM-Maringá, Paraná, Brazil.
authors.forename: Ricardo P
authors.initials: RP
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D023681:Cichlids / Q000502:physiology*; D015870:Gene Expression; D006854:Hydrocortisone / Q000378:metabolism; D010641:Phenotype; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D013312:Stress, Physiological
chemical_list: D019837:Ryanodine Receptor Calcium Release Channel; D006854:Hydrocortisone