title: Molecular Types of Methicillin-Resistant Staphylococcus aureus and Methicillin-Sensitive S. aureus Strains Causing Skin and Soft Tissue Infections and Nasal Colonization, Identified in Community Health Centers in New York City.
abstract: In November 2011, The Rockefeller University Center for Clinical and Translational Science (CCTS), the Laboratory of Microbiology and Infectious Diseases, and Clinical Directors Network (CDN) launched a research and learning collaborative project with six community health centers in the New York City metropolitan area to determine the nature (clonal type) of community-acquired Staphylococcus aureus strains causing skin and soft tissue infections (SSTIs). Between November 2011 and March 2013, wound and nasal samples from 129 patients with active SSTIs suspicious for S. aureus were collected and characterized by molecular typing techniques. In 63 of 129 patients, the skin wounds were infected by S. aureus: methicillin-resistant S. aureus (MRSA) was recovered from 39 wounds and methicillin-sensitive S. aureus (MSSA) was recovered from 24. Most-46 of the 63-wound isolates belonged to the CC8/Panton-Valentine leukocidin-positive (PVL(+)) group of S. aureus clone USA300: 34 of these strains were MRSA and 12 were MSSA. Of the 63 patients with S. aureus infections, 30 were also colonized by S. aureus in the nares: 16 of the colonizing isolates were MRSA, and 14 were MSSA, and the majority of the colonizing isolates belonged to the USA300 clonal group. In most cases (70%), the colonizing isolate belonged to the same clonal type as the strain involved with the infection. In three of the patients, the identity of invasive and colonizing MRSA isolates was further documented by whole-genome sequencing.
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Pardos de la Gandara
authors.affiliation: Department of Biochemistry and Molecular Biology, Penn State University, University Park, Pennsylvania, USA.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Raygoza Garay
authors.affiliation: Department of Biochemistry and Molecular Biology, Penn State University, University Park, Pennsylvania, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Mwangi
authors.affiliation: Clinical Directors Network (CDN), New York, New York, USA The Rockefeller University Center for Clinical and Translational Science, New York, New York, USA.
authors.forename: Jonathan N
authors.initials: JN
authors.lastname: Tobin
authors.affiliation: Clinical Directors Network (CDN), New York, New York, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Tsang
authors.affiliation: Clinical Directors Network (CDN), New York, New York, USA.
authors.forename: Chamanara
authors.initials: C
authors.lastname: Khalida
authors.affiliation: Clinical Directors Network (CDN), New York, New York, USA.
authors.forename: Brianna
authors.initials: B
authors.lastname: D'Orazio
authors.affiliation: The Rockefeller University Center for Clinical and Translational Science, New York, New York, USA.
authors.forename: Rhonda G
authors.initials: RG
authors.lastname: Kost
authors.affiliation: The Rockefeller University Center for Clinical and Translational Science, New York, New York, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Leinberger-Jabari
authors.affiliation: The Rockefeller University Center for Clinical and Translational Science, New York, New York, USA.
authors.forename: Cameron
authors.initials: C
authors.lastname: Coffran
authors.affiliation: The Rockefeller University Center for Clinical and Translational Science, New York, New York, USA.
authors.forename: Teresa H
authors.initials: TH
authors.lastname: Evering
authors.affiliation: The Rockefeller University Center for Clinical and Translational Science, New York, New York, USA.
authors.forename: Barry S
authors.initials: BS
authors.lastname: Coller
authors.affiliation: Urban Health Center, Bronx, New York, USA.
authors.forename: Shirish
authors.initials: S
authors.lastname: Balachandra
authors.affiliation: Urban Health Center, Bronx, New York, USA.
authors.forename: Tracie
authors.initials: T
authors.lastname: Urban
authors.affiliation: Urban Health Center, Bronx, New York, USA.
authors.forename: Claude
authors.initials: C
authors.lastname: Parola
authors.affiliation: Urban Health Center, Bronx, New York, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Salvato
authors.affiliation: Hudson River Health Care, Peekskill, New York, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Jenks
authors.affiliation: Open Door Family Medical Center, Ossining, New York, USA.
authors.forename: Daren
authors.initials: D
authors.lastname: Wu
authors.affiliation: Manhattan Physicians Group-125th Street Clinic, New York, New York, USA.
authors.forename: Rhonda
authors.initials: R
authors.lastname: Burgess
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Chung
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica (ITQB/UNL), Oeiras, Portugal.
authors.forename: Herminia
authors.initials: H
authors.lastname: de Lencastre
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA tomasz@rockefeller.edu.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tomasz
mesh_terms: D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology*; D003151:Community Health Centers; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016106:Methicillin Resistance; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009519:New York City / Q000453:epidemiology; D009666:Nose / Q000382:microbiology; D018461:Soft Tissue Infections / Q000453:epidemiology / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013207:Staphylococcal Skin Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014947:Wounds and Injuries / Q000382:microbiology