title: High expression of long non-coding RNA ANRIL is associated with poor prognosis in hepatocellular carcinoma.
abstract: BACKGROUND
  long non-coding RNA ANRIL (lncRNA ANRIL) has been demonstrated to play a crucial role in cancer progression. However, its effects in hepatocellular carcinoma (HCC) have not been explored. The aim of this study was to investigate the clinical significance of lncRNA NRIL in human HCC.
  
  
  METHODS
  In this study, we determined for the first time the expression of lncRNA ANRIL in human HCC by quantitative Real-time-PCR analysis. Kaplan-Meier curves and multivariate Cox proportional models were used to study the impact on clinical outcome. Small interfering RNA (siRNA) was used to silence lncRNA ANRIL and to explore the effects of reduced lncRNA ANRIL expression on cell growth and metastasis.
  
  
  RESULTS
  lncRNA ANRIL expression in HCC tissues was significantly higher than in the adjacent non-tumor tissues (P<0.05). The expression of lncRNA ANRIL was remarkably associated with the histologic grade and TNM stage of HCC patients (P<0.05). In addition, HCC patients with higher lncRNA ANRIL expression had significantly poorer overall survival (P<0.05). Multivariate analysis suggested that high lncRNA ANRIL expression was an independent predictor of poor prognosis (P<0.05). Moreover, in vitro assays revealed that the decreased expression of lncRNA ANRIL could suppress the cell proliferation, migration and invasion HCC cells.
  
  
  CONCLUSIONS
  Our results suggest that lncRNA ANRIL may serve as an efficient clinical biomarker and a therapeutic target for HCC patients.
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Long
authors.initials: L
authors.lastname: Hua
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Kun-Hou
authors.initials: KH
authors.lastname: Yao
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Wan-Li
authors.initials: WL
authors.lastname: Ma
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D014162:Transfection
chemical_list: C561518:CDKN2B antisense RNA, human; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering
keywords: Hepatocellular carcinoma; invasion; lncRNA ANRIL; migration; overall survival; proliferation