title: Effects of supercritical fluid chromatography conditions on enantioselectivity and performance of polyproline-derived chiral stationary phases.
abstract: The chromatographic behaviour and performance of four polyproline-derived chiral stationary phases (CSPs) were tested using supercritical fluid chromatography (SFC). A series of structurally related racemic compounds, whose enantioseparation was proved to be sensitive to the type of mobile phase used in NP-HPLC, were chosen to be tested in the SFC conditions. Good enantioselection ability was shown by the CSPs for the analytes tested in the new conditions. Resolution, efficiency and analysis time, were considerably improved with respect to NP-HPLC when CO2/alcohol mobile phases were used. Monolithic columns clearly show enhanced chromatographic parameters and improved performance respect to their bead-based counterparts.
authors.affiliation: University of Barcelona, Campus Torribera, E-08921 Santa Coloma de Gramenet Barcelona, Spain.
authors.forename: Arnau
authors.initials: A
authors.lastname: Novell
authors.affiliation: Waters Cromatografía S.A., Ronda Can Fatjó 7-A, 08290 Cerdanyola del Vallés Barcelona, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Méndez
authors.affiliation: University of Barcelona, Campus Torribera, E-08921 Santa Coloma de Gramenet Barcelona, Spain. Electronic address: cminguillon@ub.edu.
authors.forename: Cristina
authors.initials: C
authors.lastname: Minguillón
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D010455:Peptides / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D010455:Peptides; C011083:polyproline
keywords: Chiral stationary phases; Enantioseparation; Monolithic columns; Poly-l-proline oligomers; Supercritical fluid chromatography