title: Large-Scale Measurement of Absolute Protein Glycosylation Stoichiometry.
abstract: Protein glycosylation is one of the most important protein modifications. Glycosylation site occupancy alteration has been implicated in human diseases and cancers. However, current glycoproteomic methods focus on the identification and quantification of glycosylated peptides and glycosylation sites but not glycosylation occupancy or glycoform stoichiometry. Here we describe a method for large-scale determination of the absolute glycosylation stoichiometry using three independent relative ratios. Using this method, we determined 117 absolute N-glycosylation occupancies in OVCAR-3 cells. Finally, we investigated the possible functions and the determinants for partial glycosylation.
authors.affiliation: Department of Pathology, Johns Hopkins University, 400 North Broadway, Smith Building, Room 4011, Baltimore, Maryland 21287, United States.
authors.forename: Shisheng
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Pathology, Johns Hopkins University, 400 North Broadway, Smith Building, Room 4011, Baltimore, Maryland 21287, United States.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D006031:Glycosylation; D006801:Humans; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins