title: Chronic restraint-induced stress has little modifying effect on radiation hematopoietic toxicity in mice.
abstract: Both radiation and stresses cause detrimental effects on humans. Besides possible health effects resulting directly from radiation exposure, the nuclear plant accident is a cause of social psychological stresses. A recent study showed that chronic restraint-induced stresses (CRIS) attenuated Trp53 functions and increased carcinogenesis susceptibility of Trp53-heterozygous mice to total-body X-irradiation (TBXI), having a big impact on the academic world and a sensational effect on the public, especially the residents living in radioactively contaminated areas. It is important to investigate the possible modification effects from CRIS on radiation-induced health consequences in Trp53 wild-type (Trp53wt) animals. Prior to a carcinogenesis study, effects of TBXI on the hematopoietic system under CRIS were investigated in terms of hematological abnormality in the peripheral blood and residual damage in the bone marrow erythrocytes using a mouse restraint model. Five-week-old male Trp53wt C57BL/6J mice were restrained 6 h per day for 28 consecutive days, and TBXI (4 Gy) was given on the 8th day. Results showed that CRIS alone induced a marked decrease in the red blood cell (RBC) and the white blood cell (WBC) count, while TBXI caused significantly lower counts of RBCs, WBCs and blood platelets, and a lower concentration of hemoglobin regardless of CRIS. CRIS alone did not show any significant effect on erythrocyte proliferation and on induction of micronucleated erythrocytes, whereas TBXI markedly inhibited erythrocyte proliferation and induced a significant increase in the incidences of micronucleated erythrocytes, regardless of CRIS. These findings suggest that CRIS does not have a significant impact on radiation-induced detrimental effects on the hematopoietic system in Trp53wt mice.
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan jp2813km@nirs.go.jp m_nenoi@nirs.go.jp.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Katsube
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Ninomiya
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Vares
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8509, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Morita
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba 263-8555, Japan jp2813km@nirs.go.jp m_nenoi@nirs.go.jp.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nenoi
mesh_terms: D000818:Animals; D001835:Body Weight; D001853:Bone Marrow / Q000473:pathology / Q000528:radiation effects; D004912:Erythrocytes / Q000166:cytology / Q000528:radiation effects; D016158:Genes, p53 / Q000235:genetics; D006413:Hematopoietic System / Q000528:radiation effects*; D007103:Immobilization / Q000523:psychology*; D007962:Leukocytes / Q000166:cytology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests; D011832:Radiation Injuries; D012307:Risk Factors; D013315:Stress, Psychological; D015430:Weight Gain; D014965:X-Rays
keywords: bone marrow micronucleated erythrocytes; chronic restraint-induced stress; mouse restraint model; peripheral blood hemogram; total-body irradiation