title: [The study of protease primary specificity by statistical analysis of MALDI mass-spectra of proteolysis products].
abstract: Experimental verification for studying of proteolytic enzymes' primary specificity by statistical analysis of MALDI (matrix-assisted laser desorption/ionization) mass spectra of products obtained by protein substrates proteolysis was done by the use of proteinases with known substrate specificity (trypsin and glutamylendopeptidase). Proposed technique not requires direct determination of proteolysis products amino acid sequences, reliably establishes proteinases with anarrow substrate specificity and shows a relative tolerance for the presence in MALDI mass-spectra peaks of contaminants. It was shown that the pseudo-positive results exception requires the use of protein substrates series with the following averaging received statistical data.
authors.forename: N L
authors.initials: NL
authors.lastname: Eremeev
authors.forename: A V
authors.initials: AV
authors.lastname: Borzenkova
mesh_terms: D010447:Peptide Hydrolases / Q000737:chemistry*; D059748:Proteolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010447:Peptide Hydrolases