title: Comparative proteomic analysis in pea treated with microbial consortia of beneficial microbes reveals changes in the protein network to enhance resistance against Sclerotinia sclerotiorum.
abstract: Microbial consortia may provide protection against pathogenic ingress via enhancing plant defense responses. Pseudomonas aeruginosa PJHU15, Trichoderma harzianum TNHU27 and Bacillus subtilis BHHU100 were used either singly or in consortia in the pea rhizosphere to observe proteome level changes upon Sclerotinia sclerotiorum challenge. Thirty proteins were found to increase or decrease differentially in 2-DE gels of pea leaves, out of which 25 were identified by MALDI-TOF MS or MS/MS. These proteins were classified into several functional categories including photosynthesis, respiration, phenylpropanoid metabolism, protein synthesis, stress regulation, carbohydrate and nitrogen metabolism and disease/defense-related processes. The respective homologue of each protein identified was trapped in Pisum sativum and a phylogenetic tree was constructed to check the ancestry. The proteomic view of the defense response to S. sclerotiorum in pea, in the presence of beneficial microbes, highlights the enhanced protection that can be provided by these microbes in challenged plants.
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: akansha007@rediffmail.com.
authors.forename: Akansha
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: bhuaks29@gmail.com.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: surendrasingh.bhu@gmail.com.
authors.forename: Surendra
authors.initials: S
authors.lastname: Singh
authors.affiliation: Centre for Bioinformatics, Banaras Hindu University, Varanasi 221005, India. Electronic address: vinaysingh@bhu.ac.in.
authors.forename: Vinay
authors.initials: V
authors.lastname: Singh
authors.affiliation: Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India. Electronic address: hbs1@rediffmail.com.
authors.forename: Harikesh Bahadur
authors.initials: HB
authors.lastname: Singh
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D018532:Pisum sativum / Q000378:metabolism* / Q000382:microbiology / Q000502:physiology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D011550:Pseudomonas aeruginosa; D058441:Rhizosphere; D013312:Stress, Physiological; D014242:Trichoderma
chemical_list: D010940:Plant Proteins
keywords: Bacillus subtilis; Microbial consortium; Pseudomonas aeruginosa; Sclerotinia sclerotiorum; Trichoderma harzianum