title: Effect of Melatonin Intake on Oxidative Stress Biomarkers in Male Reproductive Organs of Rats under Experimental Diabetes.
abstract: This study investigated the antioxidant system response of male reproductive organs during early and late phases of diabetes and the influence of melatonin treatment. Melatonin was administered to five-week-old Wistar rats throughout the experiment, in drinking water (10 μg/kg b.w). Diabetes was induced at 13 weeks of age by streptozotocin (4.5 mg/100 g b.w., i.p.) and animals were euthanized with 14 or 21 weeks old. Activities of catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and lipid peroxidation were evaluated in prostate, testis, and epididymis. The enzymes activities and lipid peroxidation were not affected in testis and epididymis after one or eight weeks of diabetes. Prostate exhibited a 3-fold increase in GPx activity at short-term diabetes and at long-term diabetes there were 2- and 3-fold increase in CAT and GST, respectively (p ≤ 0.01). Melatonin treatment to healthy rats caused a 47% increase in epididymal GPx activity in 14-week-old rats. In prostate, melatonin administration normalized GST activity at both ages and mitigated GPx at short-term and CAT at long-term diabetes. The testis and epididymis were less affected by diabetes than prostate. Furthermore, melatonin normalized the enzymatic disorders in prostate demonstrating its effective antioxidant role, even at low dosages.
authors.affiliation: Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista (UNESP), Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil ; Department of Cell Biology, Institute of Biology, State University of Campinas (UNICAMP), Charles Darwin Street, Building N, 13083 863 Campinas, SP, Brazil.
authors.forename: Marina G
authors.initials: MG
authors.lastname: Gobbo
authors.affiliation: Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista (UNESP), Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil ; Department of Cell Biology, Institute of Biology, State University of Campinas (UNICAMP), Charles Darwin Street, Building N, 13083 863 Campinas, SP, Brazil.
authors.forename: Carolina F Pereira
authors.initials: CF
authors.lastname: Costa
authors.affiliation: Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista (UNESP), Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil ; Department of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista (UNESP), Crisóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Danilo G Humberto
authors.initials: DG
authors.lastname: Silva
authors.affiliation: Department of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista (UNESP), Crisóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: de Almeida
authors.affiliation: Department of Cell Biology, Institute of Biology, State University of Campinas (UNICAMP), Charles Darwin Street, Building N, 13083 863 Campinas, SP, Brazil.
authors.forename: Rejane M
authors.initials: RM
authors.lastname: Góes
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000097:blood / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D005979:Glutathione Peroxidase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D008550:Melatonin