title: Volume Changes During Active Shape Fluctuations in Cells.
abstract: Cells modify their volume in response to changes in osmotic pressure but it is usually assumed that other active shape variations do not involve significant volume fluctuations. Here we report experiments demonstrating that water transport in and out of the cell is needed for the formation of blebs, commonly observed protrusions in the plasma membrane driven by cortex contraction. We develop and simulate a model of fluid-mediated membrane-cortex deformations and show that a permeable membrane is necessary for bleb formation which is otherwise impaired. Taken together, our experimental and theoretical results emphasize the subtle balance between hydrodynamics and elasticity in actively driven cell morphological changes.
authors.affiliation: CNR-Consiglio Nazionale delle Ricerche, Istituto per l'Energetica e le Interfasi, Via Roberto Cozzi 53, 20125 Milano, Italy.|Center for Complexity and Biosystems and Department of Physics, University of Milano, Via Celoria 16, 20133 Milano, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Taloni
authors.affiliation: Departments of Biochemistry and Molecular Biology, Sciences II, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
authors.forename: Elena
authors.initials: E
authors.lastname: Kardash
authors.affiliation: CNR-Consiglio Nazionale delle Ricerche, Istituto per l'Energetica e le Interfasi, Via Roberto Cozzi 53, 20125 Milano, Italy.|CNRS, LSPM UPR3407, Université Paris 13, Sorbonne Paris Cit, 93430 Villetaneuse, France.
authors.forename: Oguz Umut
authors.initials: OU
authors.lastname: Salman
authors.affiliation: LMS, CNRS-UMR 7649, Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, France.
authors.forename: Lev
authors.initials: L
authors.lastname: Truskinovsky
authors.affiliation: CNR-Consiglio Nazionale delle Ricerche, Istituto per l'Energetica e le Interfasi, Via Roberto Cozzi 53, 20125 Milano, Italy.|Center for Complexity and Biosystems and Department of Physics, University of Milano, Via Celoria 16, 20133 Milano, Italy.|Institute for Scientific Interchange Foundation, Via Alassio 11/C, 10126 Torino, Italy.|Department of Applied Physics, Aalto University, P.O. Box 14100, FIN-00076 Aalto, Finland.
authors.forename: Stefano
authors.initials: S
authors.lastname: Zapperi
authors.affiliation: Center for Complexity and Biosystems and Department of Bioscience, University of Milano, Via Celoria 26, 20133 Milano, Italy.
authors.forename: Caterina A M
authors.initials: CA
authors.lastname: La Porta
mesh_terms: D000465:Algorithms; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism; D051400:Aquaporin 3 / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape / Q000502:physiology*; D003198:Computer Simulation; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism*; D018613:Microscopy, Confocal; D008954:Models, Biological; D009997:Osmotic Pressure; D014867:Water / Q000737:chemistry; D015027:Zebrafish; D054460:rho-Associated Kinases / Q000378:metabolism
chemical_list: D014867:Water; D051398:Aquaporin 1; D051400:Aquaporin 3; D054460:rho-Associated Kinases