title: Norlignans from Pouzolzia zeylanica var. microphylla and their nitric oxide inhibitory activity.
abstract: Five new compounds, pouzolignan F [4-hydroxy-3-(3,5-dihydroxyphenyl)-2-[bis(4-hydroxy-3-methoxyphenyl)methyl]butyl acetate] (1), pouzolignan G [4-hydroxy-3-(3,5-dihydroxyphenyl)-2-[(4-hydroxy-3,5-dimethoxyphenyl)(4-hydroxy-3-methoxyphenyl)methyl]butyl acetate] (2), pouzolignan H [1,4-dihydroxy-3-(3,5-dihydroxyphenyl)-2-[bis(4-hydroxy-3-methoxyphenyl)methyl]butane] (3), pouzolignan I [1,4-dihydroxy-3-(3,5-dihydroxyphenyl)-2-[(4-hydroxy-3,5-dime thoxyphenyl)-(4-hydroxy-3-methoxyphenyl)methyl]butane] (4), and pouzolignan J [1,4-dihydroxy-3-(3,5-dihydroxyphenyl) -2-[(3,4,5-trimethoxyphenyl)(4-hydroxy-3-methoxyphenyl)methyl]butane] (5), along with two known compounds, indolyl-3-carboxylic acid (6) and uracil (7), were isolated from the aerial parts of Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W.T.Wang. The structures of these compounds were characterized based on spectroscopic methods, including IR, NMR ((1)H-(1)H COSY, HSQC, HMBC, and NOESY), and HR-ESI/TOF-MS experiments. All the new norlignans were assayed for inhibitory activity against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse peritoneal macrophages.
authors.affiliation: a School of Traditional Chinese Medicine, Guangdong Pharmaceutical University , Guangzhou 510006 , China.
authors.forename: Zhuo-Han
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: a School of Traditional Chinese Medicine, Guangdong Pharmaceutical University , Guangzhou 510006 , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a School of Traditional Chinese Medicine, Guangdong Pharmaceutical University , Guangzhou 510006 , China.
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Tao
authors.affiliation: a School of Traditional Chinese Medicine, Guangdong Pharmaceutical University , Guangzhou 510006 , China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Luo
authors.affiliation: a School of Traditional Chinese Medicine, Guangdong Pharmaceutical University , Guangzhou 510006 , China.
authors.forename: Chu-Qian
authors.initials: CQ
authors.lastname: Zhong
authors.affiliation: a School of Traditional Chinese Medicine, Guangdong Pharmaceutical University , Guangzhou 510006 , China.
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Guo
mesh_terms: D000818:Animals; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D035261:Plant Components, Aerial / Q000737:chemistry; D027003:Urticaceae / Q000737:chemistry
chemical_list: D017705:Lignans; D008070:Lipopolysaccharides; D009569:Nitric Oxide
keywords: Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W.T.Wang; Urticaceae; nitric oxide inhibition; norlignans; pouzolignans F–J