title: Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance.
abstract: The impact of early life exposure to bisphenol A (BPA) through drinking water was investigated in mouse models of respiratory allergy, food allergy and oral tolerance. Balb/c mice were exposed to BPA (0, 10 or 100 μg/ml), and the offspring were intranasally exposed to the allergen ovalbumin (OVA). C3H/HeJ offspring were sensitized with the food allergen lupin by intragastric gavage, after exposure to BPA (0, 1, 10 or 100 μg/ml). In separate offspring, oral tolerance was induced by gavage of 5 mg lupin one week before entering the protocol for the food allergy induction. In the airway allergy model, BPA (100 μg/ml) caused increased eosinophil numbers in bronchoalveolar lavage fluid (BALF) and a trend of increased OVA-specific IgE levels. In the food allergy and tolerance models, BPA did not alter the clinical anaphylaxis or antibody responses, but induced alterations in splenocyte cytokines and decreased mouse mast cell protease (MMCP)-1 serum levels. In conclusion, early life exposure to BPA through drinking water modestly augmented allergic responses in a mouse model of airway allergy only at high doses, and not in mouse models for food allergy and tolerance. Thus, our data do not support that BPA promotes allergy development at exposure levels relevant for humans.
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: unni.cecilie.nygaard@fhi.no.
authors.forename: Unni Cecilie
authors.initials: UC
authors.lastname: Nygaard
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: nina.eriksen.vinje@gmail.com.
authors.forename: Nina Eriksen
authors.initials: NE
authors.lastname: Vinje
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: marisamuelsen@gmail.com.
authors.forename: Mari
authors.initials: M
authors.lastname: Samuelsen
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway; GenØk - Centre for Biosafety, PO Box 6418, 9294 Tromsø, Norway. Electronic address: monica.andreassen@fhi.no.
authors.forename: Monica
authors.initials: M
authors.lastname: Andreassen
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: else.carin.groeng@fhi.no.
authors.forename: Else-Carin
authors.initials: EC
authors.lastname: Groeng
authors.affiliation: Dep of Air Pollution and Noise, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: Anette.Kocbach@fhi.no.
authors.forename: Anette Kocbach
authors.initials: AK
authors.lastname: Bølling
authors.affiliation: Dep of Air Pollution and Noise, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: rune.becher@fhi.no.
authors.forename: Rune
authors.initials: R
authors.lastname: Becher
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway; Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University for Science and Technology, Trondheim, Norway. Electronic address: martloev@online.no.
authors.forename: Martinus
authors.initials: M
authors.lastname: Lovik
authors.affiliation: Dep of Food, Water and Cosmetics, Norwegian Institute of Public Health, PO Box 4404, 0403 Oslo, Norway. Electronic address: johanna.bodin@fhi.no.
authors.forename: Johanna
authors.initials: J
authors.lastname: Bodin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000633:toxicity*; D002478:Cells, Cultured; D003433:Crosses, Genetic; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity*; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000633:toxicity; D005260:Female; D005512:Food Hypersensitivity / Q000209:etiology* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007108:Immune Tolerance / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D007774:Lactation; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000008:administration & dosage / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020545:Respiratory Mucosa / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Food allergy; Intestine; Lung; Respiratory allergy; Tolerance