title: Circulating MicroRNA-21 Is a Potential Diagnostic Biomarker in Gastric Cancer.
abstract: MicroRNA-21 was upexpressed in gastric cancer (GC) indicating that it is a potential diagnostic biomarker for GC. In this study, 50 GC patients and 50 healthy controls were recruited. miR-21 levels in serum and peripheral blood mononuclear cells (PBMCs) were quantified using quantitative real-time PCR. CA199, and CEA were measured using electrochemiluminescence assay. The sensitivity and specificity of circulating miR-21, CA199 and CEA in GC diagnosis, the correlation of circulating miR-21 to clinicopathological features, and the diagnostic value of miR-21 in different GC stages were determined. The levels of miR-21 in both serum and PBMCs increased significantly in GC patients comparing to healthy controls; however, no correlation was observed between circulating miR-21 level and clinicopathological features. The sensitivity and specificity of miR-21 in serum and PBMCs, and CA199 and CEA in GC diagnosis were 88.4%, 79.6%, 81.3%, 73.4%, 60.5%, 55.9%, and 68.6%, 59.3%, respectively. The positive prediction rates of circulating miR-21 in GC stages I to IV were all around 90%, while those of CA199 and CEA were around or less than 50%. Our data suggest circulating miR-21 (both in serum and in PBMCs) can serve as a good biomarker for GC and could be used in diagnosis of early (stage I) and late GC (stage IV).
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Jiangsu University, Kunshan 215300, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Pathology, Chongqing Cancer Institute, Chongqing 400030, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Cancer Research, Central South University, Changsha 410078, China.
authors.forename: Zeyou
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Jiangsu University, Kunshan 215300, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Pathology, Chongqing Cancer Institute, Chongqing 400030, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Jiangsu University, Kunshan 215300, China.
authors.forename: XiongYong
authors.initials: X
authors.lastname: Pu
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Jiangsu University, Kunshan 215300, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C578959:carbohydrate antigen 199, human