title: Paenibacillus rhizoryzae sp. nov., isolated from rice rhizosphere.
abstract: A Gram-stain-positive, endospore-forming, rod-shaped bacterium, designated 1ZS3-5(T), was isolated from rice rhizosphere in Hunan Province, PR China. The isolate was identified as a member of the genus Paenibacillus on the basis of phenotypic characteristics and phylogenetic inference analysis. The 16S rRNA and rpoB gene (β-subunit of bacterial RNA polymerase) sequences were closely related to those of Paenibacillus taihuensis CGMCC 1.10966(T) with similarities of 97.2% and 89.7%, respectively. The DNA-DNA hybridization value between 1ZS3-5(T) and P. taihuensis CGMCC 1.10966(T) was 33.4%. The DNA G+C content of 1ZS3-5(T) was 47.5 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid and unknown phospholipid. The predominant respiratory quinone was MK-7. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. Based on these results, 1ZS3-5(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizoryzae sp. nov. is proposed. The type strain is 1ZS3-5(T) ( = ACCC 19782(T) = DSM 29322(T)).
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.|Qiyang Agro-ecosystem of National Field Experimental Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Qiyang 426182, PR China.
authors.forename: Ju-Sheng
authors.initials: JS
authors.lastname: Gao
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.|Department of Soil Science and Soil Water Conservation, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Rizwan
authors.initials: R
authors.lastname: Ali Sheirdil
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiu-Cheng
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D012275:Oryza; D056507:Paenibacillus / Q000145:classification / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid