title: Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia.
abstract: Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evaluate the influence of acute stress on metabolic activity and oxidative stress of masseter muscles of rats subjected to occlusal modification through morphological and histochemical analyses. In this study, adult Wistar rats were divided into 4 groups: a group with extraction and acute stress (E+A); group with extraction and without stress (E+C); group without extraction and with acute stress (NO+A); and control group without both extraction and stress (NO+C). Masseter muscles were analyzed by Succinate Dehydrogenase (SDH), Nicotinamide Adenine Dinucleotide Diaphorase (NADH) and Reactive Oxygen Species (ROS) techniques. Statistical analyses and two-way ANOVA were applied, followed by Tukey-Kramer tests. In the SDH test, the E+C, E+A and NO+A groups showed a decrease in high desidrogenase activities fibers (P < 0.05), compared to the NO+C group. In the NADH test, there was no difference among the different groups. In the ROS test, in contrast, E+A, E+C and NO+A groups showed a decrease in ROS expression, compared to NO+C groups (P < 0.05). Modified dental occlusion and acute stress--which are important and prevalent problems that affect the general population--are important etiologic factors in metabolic plasticity and ROS levels of masseter muscles.
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil.
authors.forename: Mamie Mizusaki
authors.initials: MM
authors.lastname: Iyomasa
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil.
authors.forename: Fernanda Silva
authors.initials: FS
authors.lastname: Fernandes
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil.
authors.forename: Daniela Mizusaki
authors.initials: DM
authors.lastname: Iyomasa
authors.affiliation: Biology Dental Buco Graduate Program, School of Dentistry of Piracicaba, University of Campinas, Piracicaba, 13414-903, SP, Brazil.
authors.forename: Yamba Carla Lara
authors.initials: YC
authors.lastname: Pereira
authors.affiliation: Department of Physiology, Medical School, University of São Paulo, Ribeirão Preto, 14040-900, SP, Brazil.
authors.forename: Rodrigo Alberto Restrepo
authors.initials: RA
authors.lastname: Fernández
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil.
authors.forename: Ricardo Alexandre
authors.initials: RA
authors.lastname: Calzzani
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil; Psychobiology Graduate Program, School of Philosophy, Science and Literature of Ribeirão Preto, University of São Paulo, Ribeirão Preto, 14040-901, SP, Brazil.
authors.forename: Glauce Crivelaro
authors.initials: GC
authors.lastname: Nascimento
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil; Psychobiology Graduate Program, School of Philosophy, Science and Literature of Ribeirão Preto, University of São Paulo, Ribeirão Preto, 14040-901, SP, Brazil.
authors.forename: Christie Ramos Andrade
authors.initials: CR
authors.lastname: Leite-Panissi
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry Faculty, University of São Paulo, Ribeirão Preto, 14040-904, SP, Brazil.
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
mesh_terms: D000818:Animals; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D008297:Male; D008406:Masseter Muscle / Q000201:enzymology / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014081:Tooth Extraction
chemical_list: D017382:Reactive Oxygen Species; D009243:NAD; D013385:Succinate Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase