title: Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model.
abstract: Bladder cancer is one of the most common malignancies of the urogenital tract. Intravesical injection of Bacillus Calmette-Guérin (BCG) is the gold standard treatment for the high-grade non-muscle invasive bladder cancer (NMIBC). However, since the treatment-related side effects are relevant, newer biological response modifiers with a better benefit/side effects ratio are needed. The tumour microenvironment can influence both tumour development and therapy efficacy. In order to obtain a good model, it is desirable to implant tumour cells in the organ from which the cancer originates. In this protocol, we describe a method for establishing a tumour in the bladder cavity of female mice and subsequent delivery of therapeutic agents; the latter are exemplified by our use of Helicobacter pylori neutrophil activating protein (HP-NAP). A preliminary chemical burn of the mucosa, followed by the injection of mouse urothelial carcinoma cell line MB49 via urethral catheterization, enables the cells to attach to the bladder mucosa. After a period, required to allow an initial proliferation of the cells, mice are treated with HP-NAP, administrated again via catheterization. The anti-tumour activity of HP-NAP is evaluated comparing the tumour volume, the extent of necrosis and the degree of vascularization between vehicle- and HP-NAP-treated animals.
authors.affiliation: Department of Biology, University of Padua.
authors.forename: Gaia
authors.initials: G
authors.lastname: Codolo
authors.affiliation: Department of Biology, University of Padua.
authors.forename: Fabio
authors.initials: F
authors.lastname: Munari
authors.affiliation: Department of Medical Diagnostic Sciences & Special Therapies, University of Padua.
authors.forename: Matteo
authors.initials: M
authors.lastname: Fassan
authors.affiliation: Department of Biology, University of Padua; marina.debernard@unipd.it.
authors.forename: Marina
authors.initials: M
authors.lastname: de Bernard
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000379:methods; D009389:Neovascularization, Pathologic / Q000628:therapy; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D014546:Urinary Catheterization / Q000379:methods
chemical_list: D001426:Bacterial Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori