title: Germ line transformation and in vivo labeling of nuclei in Diptera: report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae).
abstract: To understand how and when developmental traits of the fruit fly Drosophila melanogaster originated during the course of insect evolution, similar traits are functionally studied in variably related satellite species. The experimental toolkit available for relevant fly models typically comprises gene expression and loss as well as gain-of-function analyses. Here, we extend the set of available molecular tools to piggyBac-based germ line transformation in two satellite fly models, Megaselia abdita and Chironomus riparius. As proof-of-concept application, we used a Gateway variant of the piggyBac transposon vector pBac{3xP3-eGFPafm} to generate a transgenic line that expresses His2Av-mCherry as fluorescent nuclear reporter ubiquitously in the gastrulating embryo of M. abdita. Our results open two phylogenetically important nodes of the insect order Diptera for advanced developmental evolutionary genetics.
authors.affiliation: Centre for Organismal Studies, Universität Heidelberg, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany.
authors.forename: Francesca
authors.initials: F
authors.lastname: Caroti
authors.forename: Silvia
authors.initials: S
authors.lastname: Urbansky
authors.forename: Maike
authors.initials: M
authors.lastname: Wosch
authors.forename: Steffen
authors.initials: S
authors.lastname: Lemke
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus / Q000378:metabolism; D004175:Diptera / Q000145:classification* / Q000166:cytology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018014:Gene Transfer Techniques; D005854:Germ Cells / Q000378:metabolism