title: Osteochondral Tissue Cell Viability Is Affected by Total Impulse during Impaction Grafting.
abstract: OBJECTIVE
  Osteochondral graft transplantation has garnered significant attention because of its ability to replace the lesion with true hyaline cartilage. However, surgical impaction of the graft to anchor it into the defect site can be traumatic and lead to cell death and cartilage degeneration. This study aimed to test the hypothesis that increasing impulse magnitude during impaction of osteochondral plugs has a direct effect on loss of cell viability.
  
  
  METHODS
  In this controlled laboratory study, the impaction force was kept constant while the impulse was varied. Ninety-six osteochondral plugs were extracted from the trochlea of bovine stifle joints and were randomly assigned into 3 experimental and 1 (nonimpacted) control group. The transferred impulse of the experimental groups reflected the median and the lower and upper quartiles of preceding clinical measurements. Data were obtained at day 0, day 4, and day 8; at each point, cell viability was assessed using the Live/Dead staining kit and histological assessments were performed to visualize matrix structural changes.
  
  
  RESULTS
  After impaction, cartilage samples stayed intact and did not show any histological signs of matrix disruption. As expected, higher impulse magnitudes introduced more cell death; however, this relationship was lost at day 8 after impaction.
  
  
  CONCLUSIONS
  Impulse magnitude has a direct effect on cell viability of the graft. Because impulse magnitude is mostly governed by the press-fit characteristics of the recipient site, this study aids in the definition of optimal insertion conditions for osteochondral grafts.
authors.affiliation: Rush University Medical Center, Chicago, Illinois, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Balash
authors.affiliation: Rush University Medical Center, Chicago, Illinois, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Kang
authors.affiliation: Rush University Medical Center, Chicago, Illinois, USA.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schwenke
authors.affiliation: Rush University Medical Center, Chicago, Illinois, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Cole
authors.affiliation: Rush University Medical Center, Chicago, Illinois, USA.
authors.forename: Markus A
authors.initials: MA
authors.lastname: Wimmer
keywords: impaction; impulse; osteochondral graft; viability