title: Association of common eNOS/NOS3 polymorphisms with preeclampsia in Tunisian Arabs.
abstract: We investigated the association of endothelial nitric oxide synthase (NOS3) polymorphisms -786T>C, 27-bp repeat 4b/4a, and Glu298Asp with preeclampsia (PE). This was a case-control study involving 345 unrelated Tunisian women with PE and 289 unrelated age- and ethnically matched control women. The -786C allele was significantly increased in PA patients when compared to healthy controls (P=0.015). In contrast, MAF of Glu298Asp (P=0.103) and 4b/4a (P=0.168) were not significantly different between the study groups. Higher frequencies of heterozygous Glu298/298Asp and homozygous -786T/-786T genotypes were seen in PE cases compared to healthy subjects. The combination of genotypes 221 (-786T>C, Glu298Asp, 4a/4a) was more in PE cases compared with control women (17.68% vs. 8.36%; P=0.029). Multivariate regression analysis confirmed this association. Genetic variation at the NOS3 locus represents a genetic risk factor for increased susceptibility to PE.
authors.affiliation: Laboratory of Human Genome and Multifactorial Diseases (LR12ES07), Faculty of Pharmacy of Monastir, University of Monastir, Tunisia. Electronic address: marwabenali24@yahoo.fr.
authors.forename: Marwa
authors.initials: M
authors.lastname: Ben Ali Gannoun
authors.affiliation: Laboratory of Human Genome and Multifactorial Diseases (LR12ES07), Faculty of Pharmacy of Monastir, University of Monastir, Tunisia.
authors.forename: Hedia
authors.initials: H
authors.lastname: Zitouni
authors.affiliation: Laboratory of Human Genome and Multifactorial Diseases (LR12ES07), Faculty of Pharmacy of Monastir, University of Monastir, Tunisia.
authors.forename: Nozha
authors.initials: N
authors.lastname: Raguema
authors.affiliation: Laboratory of Human Genome and Multifactorial Diseases (LR12ES07), Faculty of Pharmacy of Monastir, University of Monastir, Tunisia.
authors.forename: Wided
authors.initials: W
authors.lastname: Maleh
authors.affiliation: Department of Hematology, Nimes University Hospital, F-30029 Nîmes Cedex 9, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Gris
authors.affiliation: Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, P.O. Box 22979 Manama, Bahrain.
authors.forename: Wassim
authors.initials: W
authors.lastname: Almawi
authors.affiliation: Laboratory of Human Genome and Multifactorial Diseases (LR12ES07), Faculty of Pharmacy of Monastir, University of Monastir, Tunisia.
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D012189:Retrospective Studies; D014416:Tunisia
chemical_list: D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Nitric oxide; Polymorphism; Preeclampsia