title: An easy way to realize SPR aptasensor: A multimode plastic optical fiber platform for cancer biomarkers detection.
abstract: The introduction of new compact systems for sensitive, fast and simplified analysis is currently playing a substantial role in the development of point-of-care solutions aimed to assist both prognosis and diagnosis. Here we report a simple and low cost biosensor based on Surface Plasmon Resonance (SPR) taking advantage of a plastic optical fiber (POF) for the detection of Vascular endothelial growth factor (VEGF), selected as a circulating protein potentially associated with cancer. Our system is based onto two crucial aspects. By one hand, the functional layer which allows the transduction signal is based on DNA aptamers, short oligonucleotide sequences that bind to non-nucleic acid targets with high affinity and specificity. By the other hand, the light guiding structure is based on a POF with a planar gold layer as the sensing region, which is particularly suitable for bioreceptors implementation. The sensor revealed to be really useful in the interface characterization. The developed system is relatively easy to realize and could well address the development of a rapid, portable and low cost diagnostic platform, with a sensitivity in the nanomolar range.
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, Aversa, Italy. Electronic address: nunzio.cennamo@unipv.it.
authors.forename: Nunzio
authors.initials: N
authors.lastname: Cennamo
authors.affiliation: Department of Chemistry, University of Pavia, Via Taramelli 12, Pavia, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Pesavento
authors.affiliation: Fondazione Bruno Kessler-LaBSSAH, Via Sommarive 18, Povo, Trento, Italy; CNR - Institute of Biophysics, Unit at Trento, Via alla Cascata 56, Trento, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lunelli
authors.affiliation: Fondazione Bruno Kessler-CMM, Via Sommarive 18, Povo, Trento, Italy.
authors.forename: Lia
authors.initials: L
authors.lastname: Vanzetti
authors.affiliation: Fondazione Bruno Kessler-LaBSSAH, Via Sommarive 18, Povo, Trento, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pederzolli
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, Aversa, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Zeni
authors.affiliation: Fondazione Bruno Kessler-LaBSSAH, Via Sommarive 18, Povo, Trento, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Pasquardini
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D055100:Optical Fibers; D010969:Plastics / Q000737:chemistry; D019095:Point-of-Care Systems; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D010969:Plastics; D042461:Vascular Endothelial Growth Factor A; D006046:Gold
keywords: Aptasensor; Cancer; Plastic optical fiber; Protein biomarkers; Surface Plasmon Resonance; Vascular endothelial growth factor