title: Mass spectrometry-based N-linked glycomic profiling as a means for tracking pancreatic cancer metastasis.
abstract: The aberrant glycosylation profile on the surface of cancer cells has been recognized for its potential diagnostic value towards assessing tumor progression. In this study, we initially investigate N-glycan profiles on the surface of normal (HPDE) and cancerous (Capan-1, Panc-1, and MIA PaCa-2) pancreatic cell lines, which are from different sites of pancreatic tumor. The enzymatically deglycosylated total N-glycans are permethylated via a quantitative solid-phase method and then analyzed by using MALDI-TOF MS and MALDI-QIT-TOF MS. We demonstrate that the level of high-mannose type glycans is higher among Capan-1 cells-pancreatic cancer cells that have metastasized to the liver-than that observed among Panc-1 and MIA PaCa-2 cells-pancreatic cancer cells from the pancreas duct head and tail regions, respectively. Furthermore, the relative abundance of highly-branched sialyted N-glycans is significantly up-regulated on Panc-1 and MIA PaCa-2 pancreatic cancer cells compared to that of normal HPDE pancreas cells. Taken together, these results indicate that specific N-glycosylation profile changes in pancreatic cancer cells can be used to not only distinguish between normal and cancerous cells but also provide more information on their location and metastatic potential.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Hae-Min
authors.initials: HM
authors.lastname: Park
authors.affiliation: Biomedical Research Institute, Korea Institute of Science and Technology (KIST), 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 136-791, South Korea.
authors.forename: Mintai Peter
authors.initials: MP
authors.lastname: Hwang
authors.affiliation: Department of Chemical Engineering, Soongsil University, 369 Sangdo-Ro, Seoul 156-743, South Korea.
authors.forename: Yoon-Woo
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Soongsil University, 369 Sangdo-Ro, Seoul 156-743, South Korea.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Biomedical Omics Team, Korea Basic Science Institute, Ochang 363-883, South Korea.
authors.forename: Jang Mi
authors.initials: JM
authors.lastname: Jin
authors.affiliation: Biomedical Omics Team, Korea Basic Science Institute, Ochang 363-883, South Korea; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon 305-333, South Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
authors.affiliation: Biomedical Research Institute, Korea Institute of Science and Technology (KIST), 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 136-791, South Korea. Electronic address: kwanhyi@kist.re.kr.
authors.forename: Kwan Hyi
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Soongsil University, 369 Sangdo-Ro, Seoul 156-743, South Korea. Electronic address: ygkim@ssu.ac.kr.
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
mesh_terms: D014408:Biomarkers, Tumor / Q000737:chemistry; D045744:Cell Line, Tumor; D006001:Glycoconjugates / Q000737:chemistry; D054794:Glycomics; D006801:Humans; D013058:Mass Spectrometry; D009362:Neoplasm Metastasis; D009584:Nitrogen / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D006001:Glycoconjugates; D011134:Polysaccharides; D009584:Nitrogen
keywords: MALDI-TOF MS; N-glycans; Pancreatic cells; Qualitative and quantitative analysis