title: Human papillomavirus infection in oral squamous cell carcinomas from Chilean patients.
abstract: Human papillomavirus (HPV) is the causal agent of cervical, anogenital and a subset of oropharyngeal carcinomas. In addition, the role of HPV in oral carcinogenesis has been suggested, although the findings are inconclusive. In this study, using conventional polymerase chain reaction (PCR) and genotyping by specific PCR and DNA sequencing, we analyzed the HPV presence in 80 oral squamous cell carcinomas (OSCCs) from Chilean subjects. In addition, we determined the expression of p16, p53, pRb and Ki-67 using immunohistochemistry (IHC). The CDKN2A (p16) promoter methylation was evaluated using methylation-specific PCR (MSP). HPV sequences were found in 9/80 (11%) OSCCs. Non-statistically significant association with p53, pRb, Ki-67 and p16 levels were found (p=0.77; 0.29; 0.83; 0.21, respectively). HPV-16 and 18 were the most prevalent HPV genotypes in 8/9 (89%) OSCCs. In addition, CDKN2A (p16) was methylated in 39% of OSCCs. No association with HPV presence (p=0.917) was found. These results suggest that HPV positive OSCCs are entities that do not resemble the molecular alterations of HPV-associated tumors in a Chilean population. More studies are warranted to determine the role of HPV in OSCCs.
authors.affiliation: Oral Pathology and Medicine Department, Dental School, Universidad de Chile, Santiago 8380492, Chile. Electronic address: montserrat.reyes.r@gmail.com.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Reyes
authors.affiliation: Oral Pathology and Medicine Department, Dental School, Universidad de Chile, Santiago 8380492, Chile. Electronic address: grojasalcayaga@yahoo.es.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Rojas-Alcayaga
authors.affiliation: Oral Pathology and Medicine Department, Dental School, Universidad de Chile, Santiago 8380492, Chile. Electronic address: pennacchiotti@gmail.com.
authors.forename: Gina
authors.initials: G
authors.lastname: Pennacchiotti
authors.affiliation: Virology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8389100, Chile. Electronic address: diegocarrillo@med.uchile.cl.
authors.forename: Diego
authors.initials: D
authors.lastname: Carrillo
authors.affiliation: Virology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8389100, Chile. Electronic address: jp_182_mb@hotmail.com.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Muñoz
authors.affiliation: Virology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8389100, Chile. Electronic address: nelsonpena@med.uchile.cl.
authors.forename: Nelson
authors.initials: N
authors.lastname: Peña
authors.affiliation: National Cancer Institute, Santiago 8380455, Chile. Electronic address: rmontesf@gmail.com.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Montes
authors.affiliation: Oral Pathology and Medicine Department, Dental School, Universidad de Chile, Santiago 8380492, Chile. Electronic address: lobos.nelson@yahoo.cl.
authors.forename: Nelson
authors.initials: N
authors.lastname: Lobos
authors.affiliation: Virology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago 8389100, Chile. Electronic address: faguayo@med.uchile.cl.
authors.forename: Francisco
authors.initials: F
authors.lastname: Aguayo
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D002677:Chile; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000473:pathology / Q000821:virology*; D009062:Mouth Neoplasms / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D011401:Promoter Regions, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral; D019394:Ki-67 Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: HPV-16/18; Oral squamous cell carcinomas; p16; p53; pRb