title: Simultaneous characterisation and quantitation of flavonol glycosides and aglycones in noni leaves using a validated HPLC-UV/MS method.
abstract: The leaves of Morinda citrifolia L. (noni) have been utilized in a variety of commercial products marketed for their health benefits. This paper reports on a rapid and selective HPLC method for simultaneous characterization and quantitation of four flavonols in an ethanolic extract of noni leaves by using dual detectors of UV (365nm) and ESI-MS (negative mode). The limits of detection and quantitation were between 0.012 and 0.165μg/mL. The intra- and inter-assay precisions, in terms of percent relative standard deviation, are less than 4.38% and 3.50%, respectively. The accuracy, in terms of recovery percentage, ranged from 96.66% to 100.03%. Good linearity (correlation coefficient >0.999) for each calibration curve of standards was achieved in the range investigated. The contents of four flavonoids in the noni leaves varied from 1.16 to 371.6mg/100g dry weight.
authors.affiliation: Research and Development Department, Tahitian Noni International, 737 East, 1180 South, American Fork, Utah 84003, USA. Electronic address: shixin_deng@tni.com.
authors.forename: Shixin
authors.initials: S
authors.lastname: Deng
authors.affiliation: Research and Development Department, Tahitian Noni International, 737 East, 1180 South, American Fork, Utah 84003, USA.
authors.forename: Brett J
authors.initials: BJ
authors.lastname: West
authors.affiliation: Research and Development Department, Tahitian Noni International, 737 East, 1180 South, American Fork, Utah 84003, USA.
authors.forename: C Jarakae
authors.initials: CJ
authors.lastname: Jensen
keywords: Flavonoids; HPLC-UV/ESI-MS; Kaempferol; Kaempferol-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside; Leaves; Morinda citrifolia L.; Noni; Quantitative determination; Quercetin; Quercetin-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside; Rutin