title: Scalable microfluidics for single-cell RNA printing and sequencing.
abstract: Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10-$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.
authors.affiliation: Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. sb3438@columbia.edu.
authors.forename: Sayantan
authors.initials: S
authors.lastname: Bose
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY, 10027, USA. wanzhenmao@gmail.com.
authors.forename: Zhenmao
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY, 10027, USA. ambrosejcarr@gmail.com.
authors.forename: Ambrose
authors.initials: A
authors.lastname: Carr
authors.affiliation: Department of Biochemistry & Molecular Biophysics, Columbia University Medical Center, New York, NY, 10032, USA. abbas.rizvi@gmail.com.
authors.forename: Abbas H
authors.initials: AH
authors.lastname: Rizvi
authors.affiliation: Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. gregorybv@gmail.com.
authors.forename: Gregory
authors.initials: G
authors.lastname: Vieira
authors.affiliation: Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. dpeer@biology.columbia.edu.|Department of Biological Sciences, Columbia University, New York, NY, 10027, USA. dpeer@biology.columbia.edu.
authors.forename: Dana
authors.initials: D
authors.lastname: Pe'er
authors.affiliation: Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. pas2182@columbia.edu.|Department of Biochemistry & Molecular Biophysics, Columbia University Medical Center, New York, NY, 10032, USA. pas2182@columbia.edu.|Sulzberger Columbia Genome Center, Columbia University Medical Center, New York, NY, 10032, USA. pas2182@columbia.edu.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Sims
mesh_terms: D045744:Cell Line, Tumor; D004129:Dimethylpolysiloxanes; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D061848:Optical Imaging; D012313:RNA / Q000302:isolation & purification; D017423:Sequence Analysis, RNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D012313:RNA