title: Assembly of bioactive multilayered nanocoatings on pancreatic islet cells: incorporation of α1-antitrypsin into the coatings.
abstract: A spontaneous multilayer deposition approach for presenting therapeutic proteins onto pancreatic islet surfaces, using a heparin polyaldehyde and glycol chitosan alternating layering scheme, has been developed to enable the nanoscale engineering of a microenvironment for transplanted cells. The nanocoating incorporating α1-antitrypsin, an anti-inflammatory protein, exhibited effective anti-coagulant activities in vitro.
authors.affiliation: Diabetes Research Group, King's College London Faculty of Life Sciences & Medicine, Guy's Campus, London, SE1 1UL, UK. z.zhi@kcl.ac.uk.
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Zhi
authors.forename: Jashandeep
authors.initials: J
authors.lastname: Singh
authors.forename: Amazon L F
authors.initials: AL
authors.lastname: Austin
authors.forename: David C D
authors.initials: DC
authors.lastname: Hope
authors.forename: Aileen J
authors.initials: AJ
authors.lastname: King
authors.forename: Shanta J
authors.initials: SJ
authors.lastname: Persaud
authors.forename: Peter M
authors.initials: PM
authors.lastname: Jones
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D000515:alpha 1-Antitrypsin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000588:Amines; D007328:Insulin; D000515:alpha 1-Antitrypsin; C118638:glycol-chitosan; D006493:Heparin; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate