title: Effect of size of bioactive glass nanoparticles on mesenchymal stem cell proliferation for dental and orthopedic applications.
abstract: Bioactive glass nanoparticles (nanostructured bioglass ceramics or nBGs) have been widely employed as a filler material for bone tissue regeneration. The physical properties of nBG particles govern their biological actions. In this study, the impact of the size of nBG particles on mouse mesenchymal stem cell (mMSC) proliferation was investigated. Three different sizes of nBG particles were prepared via the sol-gel method with varying concentrations of the surfactant and polyethylene glycol (PEG), and the particles were characterized. Increased concentrations of PEG decreased the size of nBG particles (nBG-1: 74.7±0.62 nm, nBG-2: 43.25±1.5 nm, and nBG-3: 37.6±0.81 nm). All three nBGs were non-toxic at a concentration of 20mg/mL. Increased proliferation was observed in mMSCs treated with smaller nBG particles. Differential mRNA expression of cyclin A2, B2, D1, and E1 genes induced by nBG particles was noticed in the mMSCs. nBG-1 and nBG-3 particles promoted cells in the G0/G1 phase to enter the S and G2/M phases. nBG particles activated ERK, but prolonged activation was achieved with nBG-3 particles. Among the prepared nBG particles, nBG-3 particles showed enhanced mMSC proliferation via the sustained activation of ERKs, upregulation of cyclin gene(s) expression, and promotion of cell transition from the G0/G1 phase to the S and G2/M phases. Thus, this study indicates that small nBG particles have clinical applications in dental and bone treatments as fillers or bone-tissue bond forming materials.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203 Tamil Nadu, India.
authors.forename: J
authors.initials: J
authors.lastname: Ajita
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203 Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Saravanan
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203 Tamil Nadu, India. Electronic address: selvamurugan.n@ktr.srmuniv.ac.in.
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D016213:Cyclins / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D010316:Particle Size
chemical_list: D001672:Biocompatible Materials; D016213:Cyclins; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Bioactive glass nanoparticles; Cell proliferation; Cyclins; ERK activation; Size