title: Somatic Embryogenesis: Identified Factors that Lead to Embryogenic Repression. A Case of Species of the Same Genus.
abstract: Somatic embryogenesis is a powerful biotechnological tool for the mass production of economically important cultivars. Due to the cellular totipotency of plants, somatic cells under appropriate conditions are able to develop a complete functional embryo. During the induction of somatic embryogenesis, there are different factors involved in the success or failure of the somatic embryogenesis response. Among these factors, the origin of the explant, the culture medium and the in vitro environmental conditions have been the most studied. However, the secretion of molecules into the media has not been fully addressed. We found that the somatic embryogenesis of Coffea canephora, a highly direct embryogenic species, is disrupted by the metabolites secreted from C. arabica, a poorly direct embryogenic species. These metabolites also affect DNA methylation. Our results show that the abundance of two major phenolic compounds, caffeine and chlorogenic acid, are responsible for inhibiting somatic embryogenesis in C. canephora.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, México.
authors.forename: Geovanny I
authors.initials: GI
authors.lastname: Nic-Can
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, México.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Galaz-Ávalos
authors.affiliation: Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, México.
authors.forename: Clelia
authors.initials: C
authors.lastname: De-la-Peña
authors.affiliation: Department of Chemistry, University of Guanajuato, L. de Retana 5, CP 36000 Guanajuato, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Alcazar-Magaña
authors.affiliation: Department of Chemistry, University of Guanajuato, L. de Retana 5, CP 36000 Guanajuato, Mexico.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Wrobel
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, México.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Loyola-Vargas
mesh_terms: D040503:Coffea / Q000378:metabolism*; D019175:DNA Methylation; D018744:DNA, Plant / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D058439:Plant Somatic Embryogenesis Techniques; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D010636:Phenols