title: Western blotting: an introduction.
abstract: Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in a variety of ways.
authors.affiliation: Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA, biji-kurien@omrf.org.
authors.forename: Biji T
authors.initials: BT
authors.lastname: Kurien
authors.forename: R Hal
authors.initials: RH
authors.lastname: Scofield
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D002021:Buffers; D055767:Immobilized Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D008567:Membranes, Artificial
chemical_list: D002021:Buffers; D055767:Immobilized Proteins; D008567:Membranes, Artificial