title: Blotting from PhastGel to Membranes by Ultrasound.
abstract: Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).
authors.affiliation: Department of Chemical Engineering, Ben Gurion University of the Negev, Beer-Sheva, 84105, Israel, kost@bgu.ac.il.
authors.forename: Joseph
authors.initials: J
authors.lastname: Kost
authors.forename: Aharon
authors.initials: A
authors.lastname: Azagury
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002021:Buffers; D002417:Cattle; D003101:Collodion / Q000737:chemistry; D005782:Gels; D008567:Membranes, Artificial; D009757:Nylons / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification; D000069453:Ultrasonic Waves; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002021:Buffers; D003101:Collodion; D005782:Gels; D008567:Membranes, Artificial; D009757:Nylons; D012710:Serum Albumin, Bovine; D000099166:Polyacrylamides