title: In Situ OH Generation from O2- and H2O2 Plays a Critical Role in Plasma-Induced Cell Death.
abstract: Reactive oxygen and nitrogen species produced by cold atmospheric plasma (CAP) are considered to be the most important species for biomedical applications, including cancer treatment. However, it is not known which species exert the greatest biological effects, and the nature of their interactions with tumor cells remains ill-defined. These questions were addressed in the present study by exposing human mesenchymal stromal and LP-1 cells to reactive oxygen and nitrogen species produced by CAP and evaluating cell viability. Superoxide anion (O2-) and hydrogen peroxide (H2O2) were the two major species present in plasma, but their respective concentrations were not sufficient to cause cell death when used in isolation; however, in the presence of iron, both species enhanced the cell death-inducing effects of plasma. We propose that iron containing proteins in cells catalyze O2- and H2O2 into the highly reactive OH radical that can induce cell death. The results demonstrate how reactive species are transferred to liquid and converted into the OH radical to mediate cytotoxicity and provide mechanistic insight into the molecular mechanisms underlying tumor cell death by plasma treatment.
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Xu
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Dingxing
authors.initials: D
authors.lastname: Liu
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Biqing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, United States of America.
authors.forename: Hailan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, China; Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, United States of America; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, United States of America.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Kong
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D005293:Ferritins / Q000737:chemistry / Q000378:metabolism; D005740:Gases / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000633:toxicity; D007501:Iron / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases; D013481:Superoxides / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D005740:Gases; D035683:MicroRNAs; D013481:Superoxides; D005293:Ferritins; D006861:Hydrogen Peroxide; D007501:Iron; C454461:FTH1 protein, human; D010088:Oxidoreductases