title: Modulation effects of sweroside isolated from the Lonicera japonica on melanin synthesis.
abstract: In the course of screening for the melanogenesis inhibitors, sweroside was isolated from Lonicera japonica. Its chemical structure was determined on the basis of spectroscopic analysis, including mass spectroscopy and nuclear magnetic resonance analysis. Sweroside inhibited potent melanogenesis in melan-a cells at 300μM without cytotoxicity. Also, sweroside decreased tyrosinase, tyrosinase-related protein-1 (TRP-1) and TRP-2 protein production in melan a cells. To identify the signaling pathway of sweroside, the ability of sweroside to influence Akt and extracellular signal-regulated protein kinase (ERK) activation was investigated. Sweroside induced Akt and ERK in a dose-dependent manner. In addition, the specific inhibition of the Akt and ERK signaling pathways were studied by specific inhibitor LY294002 and U0126, respectively and it was causing the increased melanin synthesis. Furthermore, sweroside presented inhibition of the body pigmentation and tyrosinase activity in zebrafish in vivo model. These results suggest that sweroside isolated from L. japonica may be an effective skin-whitening agent through the regulates the expression of MAP kinase and melanogenic enzymes.
authors.affiliation: HK Bio, Business Incubator, Daegu Haany University, Gyeongsan, Gyeongbuk 712-715, Republic of Korea.
authors.forename: Yong Tae
authors.initials: YT
authors.lastname: Jeong
authors.affiliation: HK Bio, Business Incubator, Daegu Haany University, Gyeongsan, Gyeongbuk 712-715, Republic of Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Jeong
authors.affiliation: Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: College of Herbal Bio-Industry, Daegu Haany University, Gyeongsan, Gyeongbuk 712-715, Republic of Korea. Electronic address: jinheekim@dhu.ac.kr.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D057888:Iridoid Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031239:Lonicera / Q000737:chemistry* / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010858:Pigmentation / Q000187:drug effects*; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015027:Zebrafish / Q000502:physiology
chemical_list: D057888:Iridoid Glucosides; D008543:Melanins; D010088:Oxidoreductases; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase; C049412:sweroside
keywords: Akt; Anti-melanogenesis; ERK; Lonicera japonica; Sweroside; Zebrafish