title: Structure and function of rhizosphere and non-rhizosphere soil microbial community respond differently to elevated ozone in field-planted wheat.
abstract: To assess the responses of the soil microbial community to chronic ozone (O3), wheat seedlings (Triticum aestivum Linn.) were planted in the field and exposed to elevated O3 (eO3) concentration. Three treatments were employed: (1) Control treatment (CK), AOT40=0; (2) O3-1, AOT40=1.59 ppm•h; (3) O3-2, AOT40=9.17 ppm•h. Soil samples were collected for the assessment of microbial biomass C, community-level physiological profiles (CLPPs), and phospholipid fatty acids (PLFAs). EO3 concentration significantly reduced soil microbial carbon and changed microbial CLPPs in rhizosphere soil, but not in non-rhizosphere soil. The results of the PLFAs showed that eO3 concentrations had significant effects on soil community structure in both rhizosphere and non-rhizosphere soils. The relative abundances of fungal and actinomycetous indicator PLFAs decreased in both rhizosphere and non-rhizosphere soils, while those of bacterial PLFAs increased. Thus the results proved that eO3 concentration significantly changed the soil microbial community function and composition, which would influence the soil nutrient supply and carbon dynamics under O3 exposure.
authors.affiliation: Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Ecology and Environment, State Forestry Administration, Beijing 100091, China. Electronic address: chenchen323@163.com.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: wangxk@rcees.ac.cn.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Ecology and Environment, State Forestry Administration, Beijing 100091, China.
authors.forename: He
authors.initials: H
authors.lastname: Shang
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D018533:Biomass; D002244:Carbon / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D010126:Ozone / Q000633:toxicity*; D010743:Phospholipids / Q000032:analysis; D025341:Principal Component Analysis; D012107:Research Design; D058441:Rhizosphere; D012988:Soil Microbiology; D014908:Triticum / Q000254:growth & development*
chemical_list: D000393:Air Pollutants; D005227:Fatty Acids; D010743:Phospholipids; D010126:Ozone; D002244:Carbon
keywords: CLPP; Non-rhizosphere soil; O(3); PLFA; Rhizosphere soil; Wheat