title: LASIC: Light Activated Site-Specific Conjugation of Native IgGs.
abstract: Numerous biological applications, from diagnostic assays to immunotherapies, rely on the use of antibody-conjugates. The efficacy of these conjugates can be significantly influenced by the site at which Immunoglobulin G (IgG) is modified. Current methods that provide control over the conjugation site, however, suffer from a number of shortfalls and often require large investments of time and cost. We have developed a novel adapter protein that, when activated by long wavelength UV light, can covalently and site-specifically label the Fc region of nearly any native, full-length IgG, including all human IgG subclasses. Labeling occurs with unprecedented efficiency and speed (>90% after 30 min), with no effect on IgG affinity. The adapter domain can be bacterially expressed and customized to contain a variety of moieties (e.g., biotin, azide, fluorophores), making reliable and efficient conjugation of antibodies widely accessible to researchers at large.
authors.affiliation: Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall, Philadelphia, Pennsylvania 19104, United States.
authors.forename: James Z
authors.initials: JZ
authors.lastname: Hui
authors.affiliation: Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall, Philadelphia, Pennsylvania 19104, United States.
authors.forename: Shereen
authors.initials: S
authors.lastname: Tamsen
authors.affiliation: Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall, Philadelphia, Pennsylvania 19104, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Bioengineering University of Pennsylvania 210 South 33rd Street, 240 Skirkanich Hall, Philadelphia, Pennsylvania 19104, United States.
authors.forename: Andrew
authors.initials: A
authors.lastname: Tsourkas
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry* / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D008027:Light; D011487:Protein Conformation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D007074:Immunoglobulin G