title: Ultrasensitive and feasibly achieved protein detection based on the integration of three signal amplification reactions via sharing a DNA sequence.
abstract: This communication reports a novel strategy for the detection of proteins based on the integration of three signal amplification reactions via sharing a specially designed DNA sequence. This strategy has been demonstrated by the assay of human TNF-α in the serum of ovarian cancer patients, showing potential clinical applications.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, P. R. China. genxili@nju.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
authors.forename: Lizhou
authors.initials: L
authors.lastname: Sun
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D010051:Ovarian Neoplasms / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood* / Q000235:genetics*
chemical_list: C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D004247:DNA