title: Correlation between some hematological parameters, acute phase proteins and serum immunoglobulins in experimental caprine besnoitiosis.
abstract: The present study was undertaken to investigate correlation between some hematological parameters, acute phase proteins and immunoglobulins in the experimentally infected goats with Besnoitia caprae from the time of infection till 360 days post infection (DPI). Six male goats, approximately 12-16 months old, were inoculated subcutaneously with approximately 1.3 × 10(8) bradyzoites of B. caprae and blood samples were collected at weekly intervals from the jugular vein of the goats. Total leukocyte count and differential leukocyte counts were determined. Acute phase proteins (APPs) including serum amyloid A (SAA), haptoglobin (Hp), fibrinogen and ceruloplasmin were undertaken at weekly intervals. We evaluated an enzyme-linked immunosorbent assay (ELISA) (using a somatic antigen of bradyzoite) to detect anti-B. caprae antibodies in caprine sera. Cysts were present in the skin biopsies of the distal parts of the leg of the infected goats from 28 DPI. From 30 to 360 DPI, results showed that the APPs concentrations including SAA, Hp, fibrinogen and ceruloplasmin were enhanced in the serum of infected goats. However, there were some variation in hematological parameters; the differences were not significant with those of the normal values. Some variations were seen in the levels of specific antibodies against this parasite and they had correlation with some hematological parameters and acute-phase proteins.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, PO Box 1731, 71345 Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Hosseini
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, PO Box 1731, 71345 Shiraz, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Namazi
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, PO Box 1731, 71345 Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Oryan
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, PO Box 1731, 71345 Shiraz, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Nazifi
keywords: Acute phase proteins; Besnoitia caprae; Hematological parameters; Immunoglobulin