title: Collagen cryogel cross-linked by naturally derived dialdehyde carboxymethyl cellulose.
abstract: We present the use of a natural derivative, dialdehyde carboxymethyl cellulose (DCMC) as the cross-linker for the preparation of spongy collagen cryogels by freezing-thawing method. The DCMC has been characterized by laser light scattering (LLS), showing the molecular weight of 2.38 × 10(5)g/mol. FT-IR studies demonstrate that the cross-linking reaction and the cryogenic treatment do not destroy the triple helix of collagen. SEM images indicate that the cryogel has a heterophase structure with interconnecting macropores. DSC measurements reveal that the incorporation of a very small amount of DCMC can significantly improve the thermal stability of collagen. Moreover, the cryogels exhibit fast swelling rate, and their equilibrium swelling ratio is related to DCMC content and pH-dependent. The in vitro blood-compatibility tests prove that the introduction of DCMC does not cause the reducing performance in hemolysis and blood clotting compared with pure collagen. Hence, the low-cost and non-toxic nature of DCMC confers the cryogel great potential in tissue engineering and other biomedical applications.
authors.affiliation: Department of Pharmaceutical and Bioengineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Tan
authors.affiliation: Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical and Bioengineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Changpeng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical and Bioengineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Changdao
authors.initials: C
authors.lastname: Mu
authors.affiliation: Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China; Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650031, China. Electronic address: hongli.li1982@gmail.com.
authors.forename: Hongli
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China. Electronic address: wlin@scu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D002266:Carboxymethylcellulose Sodium / Q000031:analogs & derivatives* / Q000494:pharmacology; D002417:Cattle; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D059985:Cryogels / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007834:Lasers; D011817:Rabbits; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D059985:Cryogels; D003094:Collagen; D002266:Carboxymethylcellulose Sodium
keywords: Collagen; Cross-linking; Cryogel; Dialdehyde carboxymethyl cellulose (DCMC); Laser light scattering (LLS)