title: Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2.
abstract: Our previous study has showed that co-grafted Schwann cells (SCs) promote proliferation and migration of the grafted oligodendrocyte precursor cells (OPCs). However, how the co-grafted SCs affect OPCs has not been clarified. In the present study, we confirmed that SC-induced proliferation and migration of OPCs were mediated by SC-secreted factors using SC-conditioned medium (SCM). Then, we detected several candidate factors, PDGF-AA, FGF-2, and IGF-1, in SCs and SCM, and their receptors in OPCs. Finally, by using the selective inhibitors, the effects of these candidate factors on proliferation and migration of OPCs were examined. Our results showed that SCM-stimulated proliferation and migration of OPCs could be markedly decreased by both AG1295 (the inhibitor of PDGFR) and PD173074 (the inhibitor of FGFR). Together, our study suggests that SCs affect proliferation and migration of OPCs through secreting PDGF-AA and FGF-2. Identity of these molecules not only contributes to understand the mechanism of SC-induced proliferation and migration of OPCs but also provides possible target for treatment of CNS diseases.
authors.affiliation: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.|Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Yue-Juan
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.|Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Jing-Xing
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Laboratory of Cell Biology and Molecular Genetics, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, People's Republic of China.
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Cheng
authors.affiliation: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.|Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Zheng-Rong
authors.initials: ZR
authors.lastname: Zhong
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Jiang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China. lhz233003@163.com.|Central Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China. lhz233003@163.com.
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
authors.affiliation: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China. jghu9200@163.com.|Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China. jghu9200@163.com.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I