title: LC/MS/MS determination and pharmacokinetic study of iridoid glycosides monotropein and deacetylasperulosidic acid isomers in rat plasma after oral administration of Morinda officinalis extract.
abstract: Morinda officinalis is a famous traditional Chinese medicine containing iridoid glycoside compounds, such as monotropein and deacetylasperulosidic acid. The aim of the study was to develop a novel and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of the two isomeric iridoid glycosides and then evaluate their pharmacokinetic properties in rats. Selected-reaction monitoring mode was employed for quantification of two analytes in rat plasma. The calibration curves were linear over their respective concentration range with correlation coefficient >0.995 for both analytes. Precision for monotropein and deacetylasperulosidic acid ranged from 2.5 to 11.9% relative standard deviation, and the accuracy of two analytes was -2.0-3.7 and -6.4-10.7% relative error, respectively. This method was successfully applied in pharmacokinetic study after oral administration of M. officinalis extract in rats. The results provided a basis for further research on the bioactivity of M. officinalis.
authors.affiliation: Department of Pharmacy, Jinan Maternity and Child Care Hospital, Jinan, China.
authors.forename: Chunmin
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Linyi People's Hospital, Linyi, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Urinary Surgery, the First Hospital of Qiqihaer City, Qiqihaer, China.
authors.forename: Jingchang
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Respiratory, the First Hospital of Qiqihaer City, Qiqihaer, China.
authors.forename: Xiujing
authors.initials: X
authors.lastname: Song
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006027:Glycosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D057889:Iridoid Glycosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D032066:Morinda / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006027:Glycosides; D057889:Iridoid Glycosides; D010936:Plant Extracts; C432744:asperulosidic acid
keywords: LC/MS/MS; Morinda officinalis; deacetylasperulosidic acid; monotropein; pharmacokinetics