title: Gene Expression Profiles of Chicken Embryo Fibroblasts in Response to Salmonella Enteritidis Infection.
abstract: The response of chicken to non-typhoidal Salmonella infection is becoming well characterised but the role of particular cell types in this response is still far from being understood. Therefore, in this study we characterised the response of chicken embryo fibroblasts (CEFs) to infection with two different S. Enteritidis strains by microarray analysis. The expression of chicken genes identified as significantly up- or down-regulated (≥3-fold) by microarray analysis was verified by real-time PCR followed by functional classification of the genes and prediction of interactions between the proteins using Gene Ontology and STRING Database. Finally the expression of the newly identified genes was tested in HD11 macrophages and in vivo in chickens. Altogether 19 genes were induced in CEFs after S. Enteritidis infection. Twelve of them were also induced in HD11 macrophages and thirteen in the caecum of orally infected chickens. The majority of these genes were assigned different functions in the immune response, however five of them (LOC101750351, K123, BU460569, MOBKL2C and G0S2) have not been associated with the response of chicken to Salmonella infection so far. K123 and G0S2 were the only 'non-immune' genes inducible by S. Enteritidis in fibroblasts, HD11 macrophages and in the caecum after oral infection. The function of K123 is unknown but G0S2 is involved in lipid metabolism and in β-oxidation of fatty acids in mitochondria.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Ama
authors.initials: A
authors.lastname: Szmolka
authors.affiliation: Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Wiener
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Marta Elsheimer
authors.initials: ME
authors.lastname: Matulova
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Karolina
authors.initials: K
authors.lastname: Varmuzova
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Rychlik
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis; D011201:Poultry Diseases / Q000378:metabolism / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000378:metabolism / Q000503:physiopathology*; D012477:Salmonella enteritidis / Q000502:physiology*; D059467:Transcriptome; D015854:Up-Regulation