title: Decreased expression of microRNA-130a correlates with TNF-α in the development of osteoarthritis.
abstract: OBJECTIVE
  Increased expression of tumor necrosis factor a (TNF-α) has emerged as an important inflammatory factor in osteoarthritis (OA) and other joint diseases. The study was performed to investigate whether the expression of TNF-α in human chondrocytes was regulated by miRNAs.
  
  
  METHODS
  MiRNA-130a and TNF-α expression in cartilage specimens was examined in patients with knee osteoarthritis, chondrocytes and osteoarthritis rat model. Chondrocytes were transfected with siRNAs as a gene silencing methods. Expression of genes and proteins were analyzed by real-time PCR and western blotting respectively.
  
  
  RESULTS
  Increased TNF-α and decreased miRNA-130a were observed in tissues from osteoarthritis patients. Moreover, we found a highly negitive correlation between miRNA-130a and TNF-α. Next, miRNA-130a loss-of-function increased the expression of TNF-α and promoted inflammation in chondrocytes. It was reasonable that miRNA-130a regulated a distinct underlying molecular and pathogenic mechanism of OA by forming a negative feedback loop with TNF-α. Furthermore, there were the abnormalities of bone metabolism in OA rat, which showed the miRNA-130a and TNF-α dysfunction that was one of important factors for the occurrence and development of OA.
  
  
  CONCLUSIONS
  Our results indicated that miR-130a played an important role in regulating the expression of TNF-α in human chondrocytes and identified miR-130a as a novel therapeutic target in OA.
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Zeng-Chun
authors.initials: ZC
authors.lastname: Li
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Yang-Zhou
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Gui-Xin
authors.initials: GX
authors.lastname: Sun
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Guo-Ting
authors.initials: GT
authors.lastname: Chen
authors.affiliation: Department of Emergency Trauma Surgery, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019902:Chondrocytes / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D020370:Osteoarthritis, Knee / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha
keywords: Osteoarthritis; TNF-α; bone; chondrocytes; miRNA-130a