title: Improved welan gum production by Alcaligenes sp. ATCC31555 from pretreated cane molasses.
abstract: Welan gum production by Alcaligenes sp. ATCC31555 from cane molasses was studied in batch fermentation to reduce production costs and enhance gum production. The pretreatment of cane molasses, agitation speed and the addition of supplements were investigated to optimize the process. Sulfuric acid hydrolysis was found to be the optimal pretreatment, resulting in a maximum gum concentration of 33.5 g/L, which is 50.0% higher than those obtained from the molasses' mother liquor. Agitation at 600 rpm at 30°C and addition of 10% n-dodecane following fermentation for 36 h increased the maximum gum production up to 41.0 ± 1.41 g/L, which is 49.1% higher than the greatest welan gum concentration in the literature so far. The welan gum product showed an acceptable molecular weight, similar rheological properties and better thermal stability to that obtained from glucose. These results indicate that cane molasses may be a suitable and inexpensive substrate for cost-effective industrial-scale welan gum production.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ai
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Pingru
authors.initials: P
authors.lastname: Yu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Shaozhi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: Suo
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: jufwang@scut.edu.cn.
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
mesh_terms: D000421:Alcaligenes / Q000187:drug effects / Q000378:metabolism*; D000473:Alkanes / Q000494:pharmacology; D061249:Batch Cell Culture Techniques; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D007700:Kinetics; D008965:Molasses; D008970:Molecular Weight; D010100:Oxygen / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012212:Rheology / Q000187:drug effects; D031786:Saccharum / Q000737:chemistry*; D012996:Solutions
chemical_list: D000473:Alkanes; D011135:Polysaccharides, Bacterial; D012996:Solutions; C007548:n-dodecane; D002244:Carbon; C068570:welan; D010100:Oxygen
keywords: Alcaligenes sp. ATCC31555; Cane molasses; Pretreatment; Welan gum; n-Dodecane