title: Nanoparticles Made From Xyloglucan-Block-Polycaprolactone Copolymers: Safety Assessment for Drug Delivery.
abstract: Xyloglucan-block-polycaprolactone (XGO-PCL) copolymer nanoparticles have been proposed as nanocarriers for drug delivery. However, the possible harmful effects of exposure to nanoparticles still remain a concern. Therefore, the aim of this study is to evaluate the potential toxicity of XGO-PCL nanoparticles using in vitro and in vivo assays. Cytotoxicity and genotoxicity studies were conducted on MRC-5 human fetal lung fibroblast cells upon exposure to XGO-PCL nanoparticles. No significant reduction in the cell viability and no DNA damage were observed at the different concentrations tested. Erythrocyte toxicity was assessed by the incubation of nanoparticles with human blood. XGO-PCL nanoparticles induced a hemolytic ratio of less than 1%, indicating good blood compatibility. Finally, the subacute toxicity of XGO-PCL nanoparticles (10 mg/kg/day) was evaluated in BALB/c mice when administered orally or intraperitoneally for 14 days. Results of the in vivo toxicity study showed no clinical signs of toxicity, mortality, weight loss, or hematological and biochemical alterations after treatment with nanoparticles. Also, microscopic analysis of the major organs revealed no histopathological abnormalities, corroborating the previous results. Thus, it can be concluded that XGO-PCL nanoparticles induced no effect indicative of toxicity, indicating their potential use as drug delivery systems.
authors.affiliation: *Departamento de Ciências Farmacêuticas, leticiamazzarino@yahoo.com.br.
authors.forename: Letícia
authors.initials: L
authors.lastname: Mazzarino
authors.affiliation: *Departamento de Ciências Farmacêuticas.
authors.forename: Gecioni
authors.initials: G
authors.lastname: Loch-Neckel
authors.affiliation: Departamento de Análises Clínicas, and.
authors.forename: Lorena
authors.initials: L
authors.lastname: Dos Santos Bubniak
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina 88040-900, Brazil;
authors.forename: Fabiana
authors.initials: F
authors.lastname: Ourique
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales (CERMAV, UPR-CNRS 5301), Université Grenoble Alpes, Grenoble Cedex 9, France; and.
authors.forename: Issei
authors.initials: I
authors.lastname: Otsuka
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales (CERMAV, UPR-CNRS 5301), Université Grenoble Alpes, Grenoble Cedex 9, France; and.
authors.forename: Sami
authors.initials: S
authors.lastname: Halila
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina 88040-900, Brazil;
authors.forename: Rozangela
authors.initials: R
authors.lastname: Curi Pedrosa
authors.affiliation: Departamento de Análises Clínicas, and.
authors.forename: Maria Cláudia
authors.initials: MC
authors.lastname: Santos-Silva
authors.affiliation: *Departamento de Ciências Farmacêuticas.
authors.forename: Elenara
authors.initials: E
authors.lastname: Lemos-Senna
authors.affiliation: Departamento de Química, Universidade Estadual de Maringá, Maringá, Paraná 87020-970, Brazil.
authors.forename: Edvani
authors.initials: E
authors.lastname: Curti Muniz
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales (CERMAV, UPR-CNRS 5301), Université Grenoble Alpes, Grenoble Cedex 9, France; and.
authors.forename: Redouane
authors.initials: R
authors.lastname: Borsali
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D016503:Drug Delivery Systems / Q000009:adverse effects*; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005936:Glucans / Q000633:toxicity*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009153:Mutagens / Q000633:toxicity; D053758:Nanoparticles / Q000633:toxicity*; D011091:Polyesters / Q000633:toxicity*; D011108:Polymers; D014990:Xylans / Q000633:toxicity*
chemical_list: D005936:Glucans; D009153:Mutagens; D011091:Polyesters; D011108:Polymers; D014990:Xylans; C016240:polycaprolactone; C029353:xyloglucan
keywords: cytotoxicity; genotoxicity; hemolysis; in vivo toxicity.; nanoparticle; nanotoxicology