title: microRNA regulation of the embryonic hypoxic response in Caenorhabditis elegans.
abstract: Layered strategies to combat hypoxia provide flexibility in dynamic oxygen environments. Here we show that multiple miRNAs are required for hypoxic survival responses during C. elegans embryogenesis. Certain miRNAs promote while others antagonize the hypoxic survival response. We found that expression of the mir-35 family is regulated by hypoxia in a HIF-1-independent manner and loss of mir-35-41 weakens hypoxic survival mechanisms in embryos. In addition, correct regulation of the RNA binding protein, SUP-26, a mir-35 family target, is needed for survival in chronic hypoxia. The identification of the full mRNA target repertoire of these miRNAs will reveal the miRNA-regulated network of hypoxic survival mechanisms in C. elegans.
authors.affiliation: Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Ole Maaløes Vej 5, Copenhagen, Denmark.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Kagias
authors.affiliation: 1] Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Ole Maaløes Vej 5, Copenhagen, Denmark [2] Department of Anatomy and Developmental Biology, Faculty of Biomedical and Psychological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Roger
authors.initials: R
authors.lastname: Pocock
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D015687:Cell Hypoxia / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; C541178:HIF-1 protein, C elegans; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C000607969:SUP-26 protein, C elegans; D014157:Transcription Factors; D010100:Oxygen