title: In Situ Detection of Integrin Ligands.
abstract: Integrins are cell surface receptors for cell adhesion. Integrin-mediated cell adhesion regulates various cellular processes, including cell survival, migration, proliferation, and differentiation. In vivo, ligands for integrins are immobilized within extracellular matrices, insoluble sheet-like or fibrous supramolecular complexes that associate with or surround cells. To better understand the molecular basis of integrin-mediated regulation of cellular behavior in vivo, it is of critical importance to collect information regarding the activities as well as spatial distributions of integrin ligands in situ. This unit describes a protocol for detecting the spatial distribution of the complement of integrin ligands in situ by overlaying soluble recombinant integrins.
authors.affiliation: Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka, Japan.
authors.forename: Daiji
authors.initials: D
authors.lastname: Kiyozumi
authors.affiliation: Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Sato-Nishiuchi
authors.affiliation: Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka, Japan.
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Sekiguchi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands
chemical_list: D016023:Integrins; D008024:Ligands
keywords: basement membrane; cell adhesion; extracellular matrix (ECM); integrin