title: Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy.
abstract: Simple, rapid, sensitive and specific detection of cancer cells is of great importance for early and accurate cancer diagnostics and therapy. By coupling nanotechnology and dual-aptamer target binding strategies, we developed a colorimetric assay for visually detecting cancer cells with high sensitivity and specificity. The nanotechnology including high catalytic activity of PtAuNP and magnetic separation & concentration plays a vital role on the signal amplification and improvement of detection sensitivity. The color change caused by small amount of target cancer cells (10 cells/mL) can be clearly distinguished by naked eyes. The dual-aptamer target binding strategy guarantees the detection specificity that large amount of non-cancer cells and different cancer cells (10(4) cells/mL) cannot cause obvious color change. A detection limit as low as 10 cells/mL with detection linear range from 10 to 10(5) cells/mL was reached according to the experimental detections in phosphate buffer solution as well as serum sample. The developed enzyme-free and cost effective colorimetric assay is simple and no need of instrument while still provides excellent sensitivity, specificity and repeatability, having potential application on point-of-care cancer diagnosis.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing, 100039, China; Department of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Daoqing
authors.initials: D
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; School of Food Engineering and Biological Technology, Tianjin University of Science and Technology, Tianjin 300222, China. Electronic address: yaqingliu@ciac.ac.cn.
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing, 100039, China. Electronic address: ekwang@ciac.ac.cn.
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics; D045744:Cell Line, Tumor; D003124:Colorimetry / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D018396:Mucin-1 / Q000235:genetics; D036103:Nanotechnology; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; C060192:MUC1 protein, human; D018396:Mucin-1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Breast cancer cells; Colorimetric detection; Cytosensor; Dual-aptamer; Nanomaterial