title: Anti-Androgenic Activity of Icarisid II from Epimedium Herb in Prostate Cancer LNCaP Cells.
abstract: Anti-androgens are regarded as potential therapeutic agents for the treatment of prostate cancer. We determined that an epimedium herb (EH) extract exhibited anti-androgenic activity in a luciferase assay using androgen receptor-positive prostate cancer LNCaP cells. Nine EH-derived flavonoids were examined. The results identified icarisid II as a very potent anti-androgenic EH-derived flavonoid. A quantitative RT-PCR analysis confirmed that the flavonol suppressed the expression of the androgen-responsive KLK3 gene.
authors.affiliation: Department of Infection Control and Prevention, Aichi Medical University.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Miura
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Oyama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iguchi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ito
authors.forename: Misako
authors.initials: M
authors.lastname: Baba
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shikama
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Usui
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hirano
authors.forename: Munekazu
authors.initials: M
authors.lastname: Iinuma
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Mikamo
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology / Q000627:therapeutic use*; D000728:Androgens / Q000378:metabolism*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007610:Kallikreins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008517:Phytotherapy; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017430:Prostate-Specific Antigen / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D011944:Receptors, Androgen; C060988:baohuoside I; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen