title: Runx1 and Runx3 Are Downstream Effectors of Nanog in Promoting Osteogenic Differentiation of the Mouse Mesenchymal Cell Line C3H10T1/2.
abstract: Previously, we reported that the transcription factor Nanog, which maintains the self-renewal of embryonic stem cells (ESCs), promotes the osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2 through a genome reprogramming process. In the present study, to clarify the mechanism underlying the multipotency of mesenchymal stem cells (MSCs) and to develop a novel approach to bone regenerative medicine, we attempted to identify the downstream effectors of Nanog in promoting osteogenic differentiation of mouse mesenchymal cells. We demonstrated that Runx1 and Runx3 are the downstream effectors of Nanog, especially in the early and intermediate osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2.
authors.affiliation: 1 Department of Oral and Maxillofacial Surgery, The University of Tokyo , Tokyo 113-8655, Japan .|3 Nihon University School of Dentistry , Department of Oral and Maxillofacial Surgery, Tokyo 101-8310, Japan .
authors.forename: Tadahito
authors.initials: T
authors.lastname: Saito
authors.affiliation: 2 Department of Bioengineering, Graduate School of Engineering, The University of Tokyo , Tokyo 113-8655, Japan .
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohba
authors.affiliation: 1 Department of Oral and Maxillofacial Surgery, The University of Tokyo , Tokyo 113-8655, Japan .
authors.forename: Fumiko
authors.initials: F
authors.lastname: Yano
authors.affiliation: 1 Department of Oral and Maxillofacial Surgery, The University of Tokyo , Tokyo 113-8655, Japan .
authors.forename: Ichiro
authors.initials: I
authors.lastname: Seto
authors.affiliation: 3 Nihon University School of Dentistry , Department of Oral and Maxillofacial Surgery, Tokyo 101-8310, Japan .
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Yonehara
authors.affiliation: 1 Department of Oral and Maxillofacial Surgery, The University of Tokyo , Tokyo 113-8655, Japan .
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.affiliation: 1 Department of Oral and Maxillofacial Surgery, The University of Tokyo , Tokyo 113-8655, Japan .
authors.forename: Toru
authors.initials: T
authors.lastname: Ogasawara
mesh_terms: D000818:Animals; D002460:Cell Line; D050676:Core Binding Factor Alpha 2 Subunit / Q000378:metabolism*; D050677:Core Binding Factor Alpha 3 Subunit / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D000071317:Nanog Homeobox Protein; D010012:Osteogenesis; D015398:Signal Transduction
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D050677:Core Binding Factor Alpha 3 Subunit; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; C493726:Runx1 protein, mouse; C493730:Runx3 protein, mouse