title: Sequence Analysis of lip R: A Good Method for Molecular Epidemiology of Clinical Isolates of Mycobacterium tuberculosis.
abstract: Advances in DNA sequencing have greatly enhanced the molecular epidemiology studies. In order to assess evolutionary and phylogenetic relation of Mycobacterium tuberculosis isolates several gene targets were evaluated. In this study, appropriate fragments of 5 highly variable genes (rpsL, mprA, lipR, katG, and fgd1 genes) were sequenced. The sequence data were analyzed with neighbor-joining method using mega and Geneious software. The phylogenetic trees analyzes revealed that the discriminatory power of lipR is much stronger than that observed in the other genes. lipR could distinguish between more clinical isolates. Therefore, lipR is a promising target for sequence analyzes of M. tuberculosis.
authors.affiliation: Department of Microbiology and Virology, Faculty of Medicine, Antimicrobial Resistance Research Center, Buali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Saedi
authors.forename: Masoud
authors.initials: M
authors.lastname: Youssefi
authors.forename: Hadi
authors.initials: H
authors.lastname: Safdari
authors.forename: Saman
authors.initials: S
authors.lastname: Soleimanpour
authors.forename: Parviz
authors.initials: P
authors.lastname: Marouzi
authors.forename: Kiarash
authors.initials: K
authors.lastname: Ghazvini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D017385:Sequence Homology
chemical_list: D001426:Bacterial Proteins