title: Nucleosome Organization around Pseudogenes in the Human Genome.
abstract: Pseudogene, disabled copy of functional gene, plays a subtle role in gene expression and genome evolution. The first step in deciphering RNA-level regulation of pseudogenes is to understand their transcriptional activity. So far, there has been no report on possible roles of nucleosome organization in pseudogene transcription. In this paper, we investigated the effect of nucleosome positioning on pseudogene transcription. For transcribed pseudogenes, the experimental nucleosome occupancy shows a prominent depletion at the regions both upstream of pseudogene start positions and downstream of pseudogene end positions. Intriguingly, the same depletion is also observed for nontranscribed pseudogenes, which is unexpected since nucleosome depletion in those regions is thought to be unnecessary in light of the nontranscriptional property of those pseudogenes. The sequence-dependent prediction of nucleosome occupancy shows a consistent pattern with the experimental data-based analysis. Our results indicate that nucleosome positioning may play important roles in both the transcription initiation and termination of pseudogenes.
authors.affiliation: The Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China ; School of Mathematics, Physics, and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Liu
authors.affiliation: School of Mathematics, Physics, and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Feng
authors.affiliation: The Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China ; School of Mathematics, Physics, and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: The Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China ; School of Mathematics, Physics, and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
mesh_terms: D015894:Genome, Human; D006801:Humans; D009707:Nucleosomes / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics*; D012313:RNA / Q000235:genetics; D061785:Transcription Initiation, Genetic; D014158:Transcription, Genetic
chemical_list: D009707:Nucleosomes; D012313:RNA