title: Alteration and localization of glycan-binding proteins in human hepatic stellate cells during liver fibrosis.
abstract: Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays were used to probe the alteration of GBPs in the activated HSCs and quiescent HSCs. As a result, 12 carbohydrates (e.g. Gal, GalNAc, and Man-9Glycan) showed increased signal, while seven carbohydrates (e.g. NeuAc, Lac, and GlcNAc-O-Ser) showed decreased signal in activated HSCs. Three carbohydrates (Gal, GalNAc, and NeuAc) were selected and subsequently used to validate the results of the carbohydrate microarrays as well as assess the distribution and localization of their binding proteins in HSCs and liver tissues by cy/histochemistry; the results showed that GBPs mainly distributed in the cytoplasma membrane and perinuclear region of cytoplasm. The immunocytochemistry was further used to verify some GBPs really exist in Golgi apparatus of the cells. The precision alteration and localization of GBPs referred to pathological changes in HSCs may provide pivotal information to help understand the biological functions of glycans how to exert through their recognition by a wide variety of GBPs. This study could lead to the development of new anti-fibrotic strategies.
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Liuyi
authors.initials: L
authors.lastname: Dang
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Jia
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Haoxiang
authors.initials: H
authors.lastname: Wu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Cui
authors.affiliation: Cell Engineering Research Centre and Department of Cell Biology, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Bian
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D055166:Hepatic Stellate Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000503:physiopathology; D011134:Polysaccharides / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D037102:Lectins; D011134:Polysaccharides
keywords: Alteration; Glycan-binding proteins; Hepatic stellate cells; Liver fibrosis; Localization