title: MicroRNA-138 regulates the balance of Th1/Th2 via targeting RUNX3 in psoriasis.
abstract: Psoriasis is a common chronic inflammatory and T cell-meditated autoimmune skin disease. A recent study found that Runt-related transcription factor 3 (RUNX3) is a susceptibility gene for psoriasis; however, its biological role in the disease has not been studied. RUNX3 was predicted to be the target gene of microRNA-138 (miR-138). The current research was designed to delineate the mechanism of miR-138 in regulating psoriasis via targeting RUNX3. In this study, we found that the expression of RUNX3 is increased significantly while the expression of miR-138 decreased significantly in CD4(+) T cells from psoriasis patients. Moreover, the luciferase report confirmed the targeting reaction between miR-138 and RUNX3. After transfection with the miR-138 inhibitor into CD4(+) T cells from healthy controls, we found that the inhibition of miR-138 increases RUNX3 expression and increased the ratio of Th1/Th2. Furthermore, the miR-138 mimic was transfected into CD4(+) T cells from psoriasis patients. The results showed that the overexpression of miR-138 inhibits RUNX3 expression and decreased the ratio of Th1/Th2 in CD4(+) T cells. Taken together, our study suggests that increased miR-138 regulates the balance of Th1/Th2 through inhibiting RUNX3 expression in psoriasis, providing a potential therapeutic target for psoriasis.
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Fu
authors.affiliation: Department of Otolaryngological, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Wenfa
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Otolaryngological, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Immunology, Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China.
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453000, Henan, China. Electronic address: zwtxinxiang@163.com.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Tian
mesh_terms: D050677:Core Binding Factor Alpha 3 Subunit / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D011565:Psoriasis / Q000235:genetics*; D018417:Th1 Cells / Q000276:immunology; D058408:Th1-Th2 Balance; D018418:Th2 Cells / Q000276:immunology
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; C546110:MIRN138 microRNA, human; D035683:MicroRNAs; C493729:Runx3 protein, human
keywords: CD4(+) T cells; Psoriasis; RUNX3; microRNA-138