title: Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase.
abstract: Dactyloscopy or fingerprint identification is a vital part of forensic evidence. Identification with fingerprints has been known since the finding of finger impressions on the clay surface of Babylonian legal contracts almost 4,000 years ago. The skin on the fingers and palms appears as grooves and ridges when observed under a microscope. A unique fingerprint is produced by the patterns of these friction skin ridges. Visible fingerprints can be deposited on solid surfaces. Colored inks have been used to deposit fingermarks on documents. Herein, we show that alkaline phosphatase can be used to transfer prints from fingers or palm to nitrocellulose or polyvinylidene difluoride membranes. The prints can be detected by using the nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate method of detection.
authors.affiliation: Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA, biji-kurien@omrf.org.
authors.forename: Biji T
authors.initials: BT
authors.lastname: Kurien
authors.forename: Debashish
authors.initials: D
authors.lastname: Danda
authors.forename: R Hal
authors.initials: RH
authors.lastname: Scofield
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D003101:Collodion / Q000737:chemistry*; D003878:Dermatoglyphics; D044707:Forensic Sciences / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D008567:Membranes, Artificial; D011145:Polyvinyls / Q000737:chemistry*; D013778:Tetrazolium Salts / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D000469:Alkaline Phosphatase; D003101:Collodion; D007211:Indoles; D008567:Membranes, Artificial; D011145:Polyvinyls; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C035455:5-bromo-4-chloro-3-indoxyl phosphate