title: Association of 14-bp insertion/deletion polymorphism of HLA-G gene with idiopathic recurrent miscarriages in infertility center patients in Yazd, Iran.
abstract: HLA-G is supposed to play a pivotal role in tolerance of the semi-allogeneic graft in pregnancy by inhibiting the cytotoxic functions of T and NK cells. A 14-bp insertion and/or deletion polymorphism in exon-8 has a possible role in HLA-G expression. The present study analyzed the 14-bp insertion/deletion polymorphism in normal pregnancy and recurrent miscarriage patients in order to discover a possible correlation between the 14-bp polymorphism and recurrent miscarriage (RM). In this study, genomic DNA from 200 RM patients and 200 normal fertile control individuals using the routine salting out method were isolated. Exon-8 of HLA-G gene of the two groups were amplified using polymerase chain reaction and analyzed by electrophoresis on 10% non-denaturing polyacrylamide gel electrophoresis containing ethidium bromide and visualized under ultraviolet light. HLA-G allele frequencies and genotypes in RM women and the fertile control group were compared using a Chi-square test. The results showed that there was a difference in allelic frequencies of 14-bp insertion polymorphism between fertile controls and RM patients; the frequency of +14 bp/-14 bp heterozygotes was significantly higher in RM patients as compared with fertile controls. Furthermore, the frequency of +14-bp insertion allele was significantly higher in those with RM as compared with normal fertile controls. From the findings here, it was concluded that a 14-bp insertion/deletion polymorphism in exon 8 could play a possible role in recurrent miscarriages. These results might ultimately be of significance for clinicians and those involved in understanding infertility and RM.
authors.affiliation: a Department of Immunology, Faculty of Medicine and.
authors.forename: Fateme
authors.initials: F
authors.lastname: Arjmand
authors.affiliation: a Department of Immunology, Faculty of Medicine and.|b Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences , Yazd , Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Samadi
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics* / Q000276:immunology; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D059951:HLA-G Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D054643:INDEL Mutation; D007492:Iran; D011110:Polymorphism, Genetic; D011247:Pregnancy
chemical_list: D059951:HLA-G Antigens
keywords: 14-bp insertion/deletion polymorphism; HLA-G; recurrent miscarriage; recurrent spontaneous abortion