title: Morphological and Biomechanical Differences in the Elastase and AngII apoE(-/-) Rodent Models of Abdominal Aortic Aneurysms.
abstract: An abdominal aortic aneurysm (AAA) is a potentially fatal cardiovascular disease with multifactorial development and progression. Two preclinical models of the disease (elastase perfusion and angiotensin II infusion in apolipoprotein-E-deficient animals) have been developed to study the disease during its initiation and progression. To date, most studies have used ex vivo methods to examine disease characteristics such as expanded aortic diameter or analytic methods to look at circulating biomarkers. Herein, we provide evidence from in vivo ultrasound studies of the temporal changes occurring in biomechanical parameters and macromolecules of the aortic wall in each model. We present findings from 28-day studies in elastase-perfused rats and AngII apoE(-/-) mice. While each model develops AAAs specific to their induction method, they both share characteristics with human aneurysms, such as marked changes in vessel strain and blood flow velocity. Histology and nonlinear microscopy confirmed that both elastin and collagen, both important extracellular matrix molecules, are similarly affected in their levels and spatial distribution. Future studies could make use of the differences between these models in order to investigate mechanisms of disease progression or evaluate potential AAA treatments.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN 47907, USA.
authors.forename: Evan H
authors.initials: EH
authors.lastname: Phillips
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN 47907, USA.
authors.forename: Alexa A
authors.initials: AA
authors.lastname: Yrineo
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN 47907, USA.
authors.forename: Hilary D
authors.initials: HD
authors.lastname: Schroeder
authors.affiliation: Department of Biomedical Engineering, John A. White, Jr. Engineering Hall, 790 W. Dickson Street, Suite 120, Fayetteville, AR 72701, USA.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Wilson
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN 47907, USA.
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN 47907, USA.
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Goergen
mesh_terms: D000804:Angiotensin II / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism* / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics / Q000503:physiopathology*; D001057:Apolipoproteins E / Q000235:genetics*; D001696:Biomechanical Phenomena; D001783:Blood Flow Velocity / Q000235:genetics; D001794:Blood Pressure / Q000235:genetics*; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D010196:Pancreatic Elastase / Q000235:genetics* / Q000378:metabolism; D051381:Rats
chemical_list: D001057:Apolipoproteins E; D000804:Angiotensin II; D010196:Pancreatic Elastase