title: Investigation of Gene Regulatory Networks Associated with Autism Spectrum Disorder Based on MiRNA Expression in China.
abstract: Autism spectrum disorder (ASD) comprise a group of neurodevelopmental disorders characterized by deficits in social and communication capacities and repetitive behaviors. Increasing neuroscientific evidence indicates that the neuropathology of ASD is widespread and involves epigenetic regulation in the brain. Differentially expressed miRNAs in the peripheral blood from autism patients were identified by high-throughput miRNA microarray analyses. Five of these miRNAs were confirmed through quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. A search for candidate target genes of the five confirmed miRNAs was performed through a Kyoto encyclopedia of genes and genomes (KEGG) biological pathways and Gene Ontology enrichment analysis of gene function to identify gene regulatory networks. To the best of our knowledge, this study provides the first global miRNA expression profile of ASD in China. The differentially expressed miR-34b may potentially explain the higher percentage of male ASD patients, and the aberrantly expressed miR-103a-3p may contribute to the abnormal ubiquitin-mediated proteolysis observed in ASD.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China; Department of Neurology at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan, 423000, P. R. China; Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan, 423000, P. R. China.
authors.forename: Fengzhen
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medical Genetics of China, Central South University, Changsha, Hunan,410078, P. R. China.
authors.forename: Jiada
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medical Genetics of China, Central South University, Changsha, Hunan,410078, P. R. China.
authors.forename: Zhengmao
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Information Science and Engineering, Central South University, Hunan, 410083, P. R. China; Hunan Engineering Laboratory for Advanced Control and Intelligent Automation, Hunan, 410083, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qiu
authors.affiliation: Department of Thoracic surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhuang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China; State Key Laboratory of Medical Genetics of China, Central South University, Changsha, Hunan,410078, P. R. China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan, 410008, P. R. China.
authors.forename: Beisha
authors.initials: B
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Medical Genetics of China, Central South University, Changsha, Hunan,410078, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China; State Key Laboratory of Medical Genetics of China, Central South University, Changsha, Hunan,410078, P. R. China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan, 410008, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
mesh_terms: D044466:Asian People / Q000235:genetics; D000067877:Autism Spectrum Disorder / Q000097:blood / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002681:China; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: C544749:MIRN103 microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D025801:Ubiquitin