title: Determination and analysis of the dissipation and residue of cyprodinil and fludioxonil in grape and soil using a modified QuEChERS method.
abstract: A simple and accurate method coupled with a gas chromatography-nitrogen phosphorus detector was developed to detect cyprodinil and fludioxonil in grape and soil. The accuracy and precision of the method in detecting the two fungicides were evaluated by conducting intra- and inter-day recovery experiments. The limits of detection were 0.017 mg/kg for cyprodinil and 0.030 mg/kg for fludioxonil. The limits of quantitation were 0.05 mg/kg for cyprodinil and 0.10 mg/kg for fludioxonil in grape and soil. The recoveries of the fungicides in grape and soil were investigated at three spiked levels and were found to range from 85.81 to 102.94% for cyprodinil and from 92.00 to 106.86% for fludioxonil, with relative standard deviations below 7%. Field experiments were conducted in two experimental locations in China. The half-lives of cyprodinil were 9.6-20.8 days in grape and 5.8-15.6 day in soil, and the half-lives of fludioxonil were 6.2-7.2 days in grape and 6.0-12.1 days in soil. When the cyprodinil and fludioxonil 62% water-dispersible granule formulation was sprayed at a low dosage three times, terminal residues of cyprodinil and fludioxonil were below 1.0 mg/kg in grape 14 days after harvest. This work may serve as a reference to establish the maximum residue limits for cyprodinil and fludioxonil in grape and promote the proper and safe use of these two fungicides.
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Chen
authors.forename: Xinwei
authors.initials: X
authors.lastname: Han
authors.forename: Zaihui
authors.initials: Z
authors.lastname: Yang
authors.forename: Mingming
authors.initials: M
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Song
authors.initials: S
authors.lastname: Zeng
authors.forename: Deyu
authors.initials: D
authors.lastname: Hu
authors.forename: Kankan
authors.initials: K
authors.lastname: Zhang
mesh_terms: D002681:China; D002849:Chromatography, Gas / Q000379:methods; D004149:Dioxoles / Q000032:analysis* / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D005659:Fungicides, Industrial / Q000032:analysis* / Q000737:chemistry; D009584:Nitrogen / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D010758:Phosphorus / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis* / Q000737:chemistry; D011758:Pyrroles / Q000032:analysis* / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D027843:Vitis
chemical_list: D004149:Dioxoles; D005659:Fungicides, Industrial; D010573:Pesticide Residues; D011743:Pyrimidines; D011758:Pyrroles; D012987:Soil; D012989:Soil Pollutants; D010758:Phosphorus; C108338:cyprodinil; C108339:fludioxonil; D009584:Nitrogen