title: Downregulation of TRIM21 contributes to hepatocellular carcinoma carcinogenesis and indicates poor prognosis of cancers.
abstract: The aim of our work is to clarify the clinical implication and functional role of tripartite motif 21 (TRIM21) in hepatocellular carcinoma (HCC). We validated that TRIM21 was downregulated in liver cancer samples by immunohistochemical (IHC) staining. We also demonstrated that its downregulation was associated with several clinicopathologic features such as tumor numbers, T stage, Barcelona Clinic Liver Cancer (BCLC) stage, and Cancer of the Liver Italian Program (CLIP) stage of HCC patients. Importantly, the expression of TRIM21 in tumor samples is significantly correlated with the prognosis of the patients. We further silenced TRIM21 in HCC cell HepG2 and LM3 and confirmed that TRIM21 silencing will promote cancer cell proliferation (CCK-8 assay), colony forming (plate colony-forming assay), migration (transwell assay), and the ability of antiapoptosis (annexin V-FITC/PI staining) in vitro. Then, we predicted gene sets influenced by TRIM21 by using bioinformatic tools. For the first time, we prove that TRIM21 is a potential tumor suppressor in HCC and its low expression indicates poor prognosis. Our findings provide useful insight into the mechanism of HCC origin and progression and offer clues to novel HCC therapies.
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. iamdqs@163.com.
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of Oncology, The Central Hospital of Enshi Autonomous of Prefecture, Enshi Clinical College of Wuhan University, Enshi, 445000, China. hd1234@163.com.
authors.forename: Du
authors.initials: D
authors.lastname: He
authors.affiliation: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Xingang Central Road 446, Haizhu District, Guangzhou, 510515, Guangdong Province, China. hiker00006@126.com.
authors.forename: Ke
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Immunology, School of Basic Medicine, Wuhan University, Wuhan, 430071, China. anny9655@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medicine, Wuhan University, Wuhan, 430071, China. tammyhalo@163.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. brilliant_510@126.com.
authors.forename: Jinfen
authors.initials: J
authors.lastname: Dai
authors.affiliation: Central Laboratory, Renmin Hospital of Wuhan Unviersity, Wuhan, 430060, China. lvhanlin1234@whu.edu.cn.
authors.forename: Hanlin
authors.initials: H
authors.lastname: Lv
authors.affiliation: Central Laboratory, Renmin Hospital of Wuhan Unviersity, Wuhan, 430060, China. wangxiaochen001@yeah.net.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Xingang Central Road 446, Haizhu District, Guangzhou, 510515, Guangdong Province, China. guoan_66@163.com.
authors.forename: Guoan
authors.initials: G
authors.lastname: Xiang
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Institute for Gastroenterology and Hepatology, Wuhan University, Jiefang Road 238, Wuhan, 430060, Hubei Province, China. yhg_rmhwh@163.com.
authors.forename: Honggang
authors.initials: H
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D012261:Ribonucleoproteins / Q000096:biosynthesis* / Q000235:genetics; D000099207:SS-A Antigen
chemical_list: D014408:Biomarkers, Tumor; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
keywords: Cancer; Hepatocellular carcinoma; Prognosis; TRIM21; Tumor suppressor