title: A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity.
abstract: Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97×10(4) M(-1). Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway.
authors.affiliation: Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: r.vikneswaran@yahoo.com.my.
authors.forename: R
authors.initials: R
authors.lastname: Vikneswaran
authors.affiliation: Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors.forename: Muhamad Syamir
authors.initials: MS
authors.lastname: Syafiq
authors.affiliation: Department of Chemistry, Sciences & Arts College - Rabigh, King Abdulaziz University, Rabigh, Saudi Arabia.
authors.forename: Naser Eltaher
authors.initials: NE
authors.lastname: Eltayeb
authors.affiliation: Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohd Naqiuddin
authors.initials: MN
authors.lastname: Kamaruddin
authors.affiliation: Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: rameshtsubra@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Ramesh
authors.affiliation: Department of Chemistry, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: rosiyah@um.edu.my.
authors.forename: R
authors.initials: R
authors.lastname: Yahya
mesh_terms: D015374:Biosensing Techniques; D003300:Copper / Q000032:analysis* / Q000302:isolation & purification; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects; D003851:Deoxyribonucleases / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D057230:Limit of Detection; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000494:pharmacology; D013890:Thiourea / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D012545:Schiff Bases; D013438:Sulfhydryl Compounds; D003300:Copper; D004247:DNA; D003851:Deoxyribonucleases; D013890:Thiourea
keywords: Chemical nucleases; Chemosensor; DNA binding; Fluorescent; Thiosemicarbazone