title: [Cys-containing peptides cause migration of monocytes].
abstract: Automated Fmoc solid-phase technique was used to synthesize Cys-containing linear peptide fragments of monocyte chemoattractant protein-1 and chemokine domain of fractalkine along with their analogues with Cys residue being either modified or replaced with Ser. Chimeric symmetric and asymmetric disulfides were also prepared from the former linear precursors. A SAR study on a set of the newly synthesized peptides revealed that capacity to stimulate migration of monocytes and to influence cell motility in vitro, in general, critically depends on the presence of Cys free thiol group in the molecule. Notably, all analogs lacking this feature, including chimeric disulfides, demonstrated lack of effect on monocyte migration.
authors.forename: M V
authors.initials: MV
authors.lastname: Sidorova
authors.forename: T I
authors.initials: TI
authors.lastname: Arefieva
authors.forename: M E
authors.initials: ME
authors.lastname: Palkeeva
authors.forename: A S
authors.initials: AS
authors.lastname: Molokoedov
authors.forename: A A
authors.initials: AA
authors.lastname: Az'muko
authors.forename: N Yu
authors.initials: NY
authors.lastname: Ruleva
authors.forename: E A
authors.initials: EA
authors.lastname: Pylaeva
authors.forename: T L
authors.initials: TL
authors.lastname: Krasnikova
authors.forename: D
authors.initials: D
authors.lastname: Bespalova
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D018932:Chemokine CCL2; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D010455:Peptides