title: Specific humoral response of hosts with variable schistosomiasis susceptibility.
abstract: The schistosome blood flukes are some of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody-secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens increased. The local antibody response in rat skin and lung, measured with ASC probes, increased after parasite migration and contributed antigen-specific antibodies to the multivalent serological response. In addition, the temporal variation of anti-parasite serum antibodies after infection and reinfection followed patterns that appear related to the antigen driving the response. Among the 29 antigens differentially recognized by the infected hosts were numerous known vaccine candidates, drug targets and several S. japonicum homologs of human schistosomiasis resistance markers-the tegument allergen-like proteins. From this set, we prioritized eight proteins that may prove to be novel schistosome vaccine and diagnostic antigens.
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Patrick
authors.initials: P
authors.lastname: Driguez
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
authors.forename: Hamish E G
authors.initials: HE
authors.lastname: McWilliam
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, Queensland Tropical Health Alliance Laboratory, James Cook University, Cairns, Queensland, Australia.|Centro de Pesquisas René Rachou, Oswaldo Cruz Foundation, Belo Horizonte, Brazil.
authors.forename: Soraya
authors.initials: S
authors.lastname: Gaze
authors.affiliation: School of Applied Sciences and Engineering, Federation University, Churchill, Victoria, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Piedrafita
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, Queensland Tropical Health Alliance Laboratory, James Cook University, Cairns, Queensland, Australia.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Pearson
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA.
authors.forename: Rie
authors.initials: R
authors.lastname: Nakajima
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Mary
authors.initials: M
authors.lastname: Duke
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Angela
authors.initials: A
authors.lastname: Trieu
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Denise L
authors.initials: DL
authors.lastname: Doolan
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.|Institute for Molecular Biosciences, University of Queensland, St Lucia, Queensland, Australia.
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Cardoso
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA.
authors.forename: Algis
authors.initials: A
authors.lastname: Jasinskas
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Geoffrey N
authors.initials: GN
authors.lastname: Gobert
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA.
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, Queensland Tropical Health Alliance Laboratory, James Cook University, Cairns, Queensland, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Loukas
authors.affiliation: Department of Microbiology, School of Biomedical Sciences, Monash University, Melbourne, Victoria, Australia.
authors.forename: Els
authors.initials: E
authors.lastname: Meeusen
authors.affiliation: Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D004198:Disease Susceptibility / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D056724:Immunity, Humoral / Q000276:immunology*; D051379:Mice; D010271:Parasites / Q000276:immunology; D040081:Protein Array Analysis; D012372:ROC Curve; D017208:Rats, Wistar; D012549:Schistosoma japonicum / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology* / Q000469:parasitology*; D014612:Vaccines
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D014612:Vaccines