title: Stem Cell-Derived Bioactive Materials Accelerate Development of Porcine In Vitro-Fertilized Embryos.
abstract: Stem cells show the capability to proliferate in an undifferentiated state with long-term self-renewal, which gives the cells advantages for use as bioactive material (BM) for embryo culture in vitro. The objective of this experiment was to investigate the effect of two BMs-human adipose tissue-derived mesenchymal stem cell BM (hAT-MSC-BM) and human embryonic stem cell-derived BM (hESC-BM)-on porcine embryo development compared to commonly used bovine serum albumin (BSA) or serum treatment groups. In vitro-fertilized (IVF) embryos were cultured in PZM-5 with 4 mg/mL BSA until day 4 and equally divided into four groups. Starting from day 4 (until day 6), each group was treated with the following protein additives: 4 mg/mL BSA (control), 10% fetal bovine serum (FBS), 10% hAT-MSC-BM, or 10% hESC-BM. Our results show FBS- and two other BM-treated groups showed significant increases in blastocyst formation rate, hatching rate, and total cell number compared with the control group (p<0.05). The hAT-MSC-BM and hESC-BM treatment groups presented better-quality embryo development, especially from the middle expanding stage to hatching. In particular, the hAT-MSC-BM-treated group showed the highest developmental potential of all groups and formed the most expanding-stage blastocysts. The relative expression of reprogramming-related transcription factor (POU5F1, SOX2, DPPA5, and CDH1), antioxidant (PRDX5), and apoptosis (BCL2L1 and BIRC5) genes also increased in two types of BMs compared to the control. In addition, we investigated the protein synthesis of the tight junction- and gap junction-related genes, connexin 43 and zonula occludens-1 (ZO-1); these increased more than in the control. These results demonstrate that stem cell-derived BMs accelerate porcine preimplantation embryo development and that the BMs would be helpful in the development of preimplantation embryos.
authors.affiliation: 1 Stem Cell Research Center, Jeju National University , Jeju Special Self-Governing Province 690-756, Korea.|2 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju Special Self-Governing Province 690-781, Korea.
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: 2 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju Special Self-Governing Province 690-781, Korea.
authors.forename: Jeremiah Ji-Man
authors.initials: JJ
authors.lastname: Moon
authors.affiliation: 1 Stem Cell Research Center, Jeju National University , Jeju Special Self-Governing Province 690-756, Korea.|2 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju Special Self-Governing Province 690-781, Korea.|3 Mirae Cell Bio , Seoul 143-854, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: 1 Stem Cell Research Center, Jeju National University , Jeju Special Self-Governing Province 690-756, Korea.|2 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju Special Self-Governing Province 690-781, Korea.|3 Mirae Cell Bio , Seoul 143-854, Korea.
authors.forename: Se-Pill
authors.initials: SP
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000187:drug effects / Q000235:genetics; D002457:Cell Extracts / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D054464:Peroxiredoxins / Q000187:drug effects / Q000235:genetics; D013552:Swine; D014157:Transcription Factors / Q000187:drug effects / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; D002457:Cell Extracts; D036341:Intercellular Signaling Peptides and Proteins; D014157:Transcription Factors; C515974:PRDX5 protein, human; D054464:Peroxiredoxins