title: Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study.
abstract: Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG-LA complexes. BLG-LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60 °C, 10 min) enhanced BLG-LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG-LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1-6:1). Absorbance at 600 nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1-2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337-364 nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas de la Republica Argentina, Argentina; Grupo de Biocoloides, Instituto de Tecnología de Alimentos, Universidad Nacional del Litoral, 1 de mayo 3250, 3000 Santa Fe, Argentina.
authors.forename: Adrián A
authors.initials: AA
authors.lastname: Perez
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas de la Republica Argentina, Argentina; Grupo de Biocoloides, Instituto de Tecnología de Alimentos, Universidad Nacional del Litoral, 1 de mayo 3250, 3000 Santa Fe, Argentina.
authors.forename: Osvaldo E
authors.initials: OE
authors.lastname: Sponton
authors.affiliation: Grupo de Biocoloides, Instituto de Tecnología de Alimentos, Universidad Nacional del Litoral, 1 de mayo 3250, 3000 Santa Fe, Argentina.
authors.forename: Romina B
authors.initials: RB
authors.lastname: Andermatten
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas de la Republica Argentina, Argentina; Grupo de Biocoloides, Instituto de Tecnología de Alimentos, Universidad Nacional del Litoral, 1 de mayo 3250, 3000 Santa Fe, Argentina.
authors.forename: Amelia C
authors.initials: AC
authors.lastname: Rubiolo
authors.affiliation: Grupo de Biocoloides, Instituto de Tecnología de Alimentos, Universidad Nacional del Litoral, 1 de mayo 3250, 3000 Santa Fe, Argentina. Electronic address: lsanti@fiq.unl.edu.ar.
authors.forename: Liliana G
authors.initials: LG
authors.lastname: Santiago
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D005231:Fatty Acids, Unsaturated; D007782:Lactoglobulins; D011134:Polysaccharides; D010368:Pectins
keywords: Binding properties; Biopolymer nanoparticles; High methoxyl pectin; Linoleic acid; Vehiculization; β-Lactoglobulin