title: Selective solid-phase extraction using molecular imprinted polymer for the analysis of diethylstilbestrol.
abstract: A simple imprinted amino-functionalized silica gel material was synthesized by combining a surface molecular imprinting technique with a sol-gel process for solid-phase extraction-high performance liquid chromatography (SPE-HPLC) determination of diethylstilbestrol (DES). Activated silica gel was used as the supporter and non-imprinted silica sorbent was synthesized without the addition of DES using the same procedure as that of DES-imprinted silica sorbent. Compared with non-imprinted polymer particles, the prepared DES-imprinted silica sorbent showed high adsorption capacity, significant selectivity, good site accessibility and fast binding kinetics for DES. The maximum static adsorption capacity of the DES-imprinted and non-imprinted silica sorbent for DES was 62.58mgg(-1) and 19.89mgg(-1), respectively. The relatively selective factor value of this DES-imprinted silica sorbent was 61.7 at the level of 50mgL(-1). And the uptake kinetics was fairly rapid so that the adsorbent equilibrium was achieved within 10min. Furthermore, the DES-imprinted polymers were used as the sorbent in solid-phase extraction to determine DES in fish samples. The MIP-SPE-HPLC method showed higher selectivity and good recoveries higher than 87.5% (R.S.D. 11.6%).
authors.affiliation: College of Chemistry and Chemical Engineering, Lanzhou University, No. 222 Tianshui Road, Lanzhou, Gansu 730000, PR China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Chemical Engineering, Lanzhou University, No. 222 Tianshui Road, Lanzhou, Gansu 730000, PR China.
authors.forename: Chuande
authors.initials: C
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Chemical Engineering, Lanzhou University, No. 222 Tianshui Road, Lanzhou, Gansu 730000, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Chemical Engineering, Lanzhou University, No. 222 Tianshui Road, Lanzhou, Gansu 730000, PR China. Electronic address: zhanghx@lzu.edu.cn.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Lanzhou University, No. 222 Tianshui Road, Lanzhou, Gansu 730000, PR China.
authors.forename: Mancang
authors.initials: M
authors.lastname: Liu
keywords: Diethylstilbestrol; High performance liquid chromatography; Molecularly imprinted polymers; Solid-phase extraction; Surface imprinted technique