title: [Tyramine and tryptamine as ligands for medical and biotechnological affinity sorbents].
abstract: A novel technique for preparation affinity sorbent based on tyramine and tryptamine was proposed. It was shown that tryptamine-Sepharose and tyramine-Sepharose effectively bind IgG, IgA, lipoprotein (a) (Lp(a)) and low density lipoproteins (LDL) from blood plasma. The sorption capacity is 4-9 mg of IgG, 2-4 mg IgA, 3-5:mg of Lp(a) and 5-7 mg of LDL per mL of gel. It was found that new sorbents can bind Lp(a) and IgG as themselves or in a complex of Lp(a) with IgG. The existence of this complex may indicate the presence of anti-Lp(a) autoantibodies in the blood of some patients. The advantages of new sorbents are easiness of its synthesis and stability during use and storage. In practice they can be applied for medical and biotechnological purposes where it is necessary to bind Lp(a), LDL, IgG, IgA.
authors.forename: P A
authors.initials: PA
authors.lastname: Levashov
authors.forename: E D
authors.initials: ED
authors.lastname: Ovchinnikova
authors.forename: M I
authors.initials: MI
authors.lastname: Afanasjev
authors.forename: D A
authors.initials: DA
authors.lastname: Fried
authors.forename: A A
authors.initials: AA
authors.lastname: Azmuko
authors.forename: I Yu
authors.initials: IY
authors.lastname: Adamova
authors.forename: S N
authors.initials: SN
authors.lastname: Pokrovsky
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007070:Immunoglobulin A / Q000737:chemistry / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification; D008024:Ligands; D017270:Lipoprotein(a) / Q000737:chemistry / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000302:isolation & purification; D014363:Tryptamines / Q000737:chemistry*; D014439:Tyramine / Q000737:chemistry*
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008024:Ligands; D017270:Lipoprotein(a); D008077:Lipoproteins, LDL; D014363:Tryptamines; C030820:tryptamine; D014439:Tyramine