title: Hydrophilic polyurethane matrix promotes chondrogenesis of mesenchymal stem cells.
abstract: Segmental polyurethanes exhibit biphasic morphology and can control cell fate by providing distinct matrix guided signals to increase the chondrogenic potential of mesenchymal stem cells (MSCs). Polyethylene glycol (PEG) based hydrophilic polyurethanes can deliver differential signals to MSCs through their matrix phases where hard segments are cell-interactive domains and PEG based soft segments are minimally interactive with cells. These coordinated communications can modulate cell-matrix interactions to control cell shape and size for chondrogenesis. Biphasic character and hydrophilicity of polyurethanes with gel like architecture provide a synthetic matrix conducive for chondrogenesis of MSCs, as evidenced by deposition of cartilage-associated extracellular matrix. Compared to monophasic hydrogels, presence of cell interactive domains in hydrophilic polyurethanes gels can balance cell-cell and cell-matrix interactions. These results demonstrate the correlation between lineage commitment and the changes in cell shape, cell-matrix interaction, and cell-cell adhesion during chondrogenic differentiation which is regulated by polyurethane phase morphology, and thus, represent hydrophilic polyurethanes as promising synthetic matrices for cartilage regeneration.
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
authors.forename: Sandeep M
authors.initials: SM
authors.lastname: Nalluri
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
authors.forename: G Rajesh
authors.initials: GR
authors.lastname: Krishnan
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
authors.forename: Calvin
authors.initials: C
authors.lastname: Cheah
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Arzumand
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
authors.forename: Caley A
authors.initials: CA
authors.lastname: Richardson
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY 14214, USA.
authors.forename: Shuying
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA; Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA. Electronic address: debanjan@buffalo.edu.
authors.forename: Debanjan
authors.initials: D
authors.lastname: Sarkar
mesh_terms: D002356:Cartilage / Q000166:cytology; D002450:Cell Communication; D002454:Cell Differentiation; D018914:Cells, Immobilized / Q000166:cytology; D020219:Chondrogenesis; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D011140:Polyurethanes; D011092:Polyethylene Glycols
keywords: Chondrogenic differentiation; Gel; Mesenchymal stem cell; Polyurethane