title: miR-892a regulated PPP2R2A expression and promoted cell proliferation of human colorectal cancer cells.
abstract: Colorectal cancer (CRC) is one of the most common malignancy cancers in the world. Aberrant microRNA expression is involved in human diseases including cancer. In the present study, we investigated the miR-892a's role in CRC cell proliferation. We found that miR-892a was frequently upregulated in human colorectal cancer tissues and cell lines compared with the matched tumor adjacent tissues and normal colonic cell line FHC. Overexpression of miR-892a promoted cell proliferation and colony formation of CRC. Bioinformatics analysis further revealed PPP2R2A was identified as a potential miR-892a. Overexpression of miR-892a-in SW480 cells reduced PPP2R2A protein expression. Subsequently, data from luciferase reporter assays showed that PPP2R2A 3'-untranslated region (3'-UTR) carried the directly binding site of miR-892a. Furthermore, siRNA-mediated silencing of PPP2R2A blocked the inhibitory effect of miR-892a-in on CRC cell growth. In sum, our data provided compelling evidence that overexpression of miR-892a may provide a selective growth promotion for CRC cells by direct suppression of PPP2R2A expression.
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Wen-long
authors.initials: WL
authors.lastname: Liang
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China. Electronic address: jiecaodoctor@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Wang-lin
authors.initials: WL
authors.lastname: Li
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, 1, Panfu Road, Yuexiu District, Guangzhou 510180, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000235:genetics; D054648:Protein Phosphatase 2 / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C574666:MIRN892 microRNA, human; D035683:MicroRNAs; C512974:PPP2R2A protein, human; D054648:Protein Phosphatase 2
keywords: Cell proliferation; Colorectal cancer; PPP2R2A; miR-892a