title: Rapid and mild purification method for nanoparticles from a dispersed solution using a monolithic silica disk.
abstract: A rapid and mild purification method for nanoparticles using the commercially available monolithic silica disk, MonoSpin(®), was developed. The nanoparticles were purified from a dispersed solution by filtration with the aid of centrifugation at 2290×g for 2min. The purification conditions were rapid, mild, and simple compared with those of the conventional purification methods such as ultracentrifugation, dialysis, size exclusion chromatography, and ultrafiltration. The method was shown to be applicable for the purification of various nanoparticles, regardless of their size (from 21 to 100nm), composition material (silica, polyethylene glycol, and pegylated liposome), and encapsulated molecule (rhodamine 110 and doxorubicin). It was shown that this method is applicable to the purification of a wide range of nanoparticles in many different fields.
authors.affiliation: Graduate School of Pharmaceutical Sciences and GPLLI Program, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Itoh
authors.affiliation: Graduate School of Pharmaceutical Sciences and GPLLI Program, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Santa
authors.affiliation: Graduate School of Pharmaceutical Sciences and GPLLI Program, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: masaru-kato@umin.ac.jp.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kato
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D005374:Filtration; D008081:Liposomes; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D014461:Ultracentrifugation
chemical_list: D008081:Liposomes; D012996:Solutions; D011092:Polyethylene Glycols; D012822:Silicon Dioxide
keywords: Centrifuge; Monolith; Nanoparticle; Purification