title: Time-dependent bladder tissue regeneration using bilayer bladder acellular matrix graft-silk fibroin scaffolds in a rat bladder augmentation model.
abstract: With advances in tissue engineering, various synthetic and natural biomaterials have been widely used in tissue regeneration of the urinary bladder in rat models. However, reconstructive procedures remain insufficient due to the lack of appropriate scaffolding, which should provide a waterproof barrier function and support the needs of various cell types. To address these problems, we have developed a bilayer scaffold comprising a porous network (silk fibroin [SF]) and an underlying natural acellular matrix (bladder acellular matrix graft [BAMG]) and evaluated its feasibility and potential for bladder regeneration in a rat bladder augmentation model. Histological (hematoxylin and eosin and Masson's trichrome staining) and immunohistochemical analyses demonstrated that the bilayer BAMG-SF scaffold promoted smooth muscle, blood vessel, and nerve regeneration in a time-dependent manner. At 12weeks after implantation, bladders reconstructed with the BAMG-SF matrix displayed superior structural and functional properties without significant local tissue responses or systemic toxicity. These results demonstrated that the bilayer BAMG-SF scaffold may be a promising scaffold with good biocompatibility for bladder regeneration in the rat bladder augmentation model.
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Urology, Jiaxing First Hospital, Jiaxing, Zhejiang Province 314001, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jia-Sheng
authors.initials: JS
authors.lastname: Wu
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Xiao
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China. Electronic address: zhongwang2000@sina.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, China. Electronic address: zhuyingjian_sjtu@126.com.
authors.forename: Ying-Jian
authors.initials: YJ
authors.lastname: Zhu
authors.affiliation: Department of Urology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China. Electronic address: lumujun@163.com.
authors.forename: Mu-Jun
authors.initials: MJ
authors.lastname: Lu
mesh_terms: D000818:Animals; D015653:Cystectomy / Q000379:methods; D005109:Extracellular Matrix / Q000737:chemistry* / Q000637:transplantation; D005349:Fibroins / Q000737:chemistry*; D008297:Male; D011474:Prosthesis Design; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures / Q000295:instrumentation; D012038:Regeneration / Q000502:physiology*; D013552:Swine; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D001743:Urinary Bladder / Q000737:chemistry / Q000254:growth & development* / Q000601:surgery*
chemical_list: D005349:Fibroins
keywords: Bladder acellular matrix graft; Bladder augmentation; Silk fibroin; Tissue engineering