title: Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum.
abstract: Besides hematological analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined from mouse blood samples. For most of these tests, plasma or serum samples are used. Data obtained by these investigations provide indications of genotype effects on metabolism and organ functions. Here we describe in detail the considerations that have to be taken into account to get adequate samples for plasma or serum analyses and the recommended sample processing for different investigations. Furthermore, we describe established methods used in the German Mouse Clinic (GMC) to determine clinical chemical parameters; for more in-depth analysis of specific classes of biomarkers, we provide instructions for ELISAs (sandwich and competitive) as well as LC-MS/MS, focusing on markers associated with bone or steroid metabolism in the mouse as working examples. Curr. Protoc. Mouse Biol. 3:69-100 © 2013 by John Wiley & Sons, Inc.
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.|Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Rathkolb
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hans
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Prehn
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Fuchs
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gailus-Durner
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Aigner
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.|Institute of Experimental Genetics, Life and Food Science Center Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Adamski
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.|Institute of Experimental Genetics, Life and Food Science Center Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.|German Research Center for Diabetes Research, Neuherberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hrabě de Angelis
keywords: ELISA; PINP; clinical chemistry; mass spectrometry; mouse; osteocalcin; steroids