title: Association of Type 2 Diabetes Mellitus related SNP genotypes with altered serum adipokine levels and metabolic syndrome phenotypes.
abstract: The pathogenesis of T2DM involves secretion of several pro-inflammatory molecules by the dramatically increased adipocytes, both by number and size, and associated macrophages of adipose tissue. Since T2DM is usually preceded by obesity and chronic systemic inflammation, the objective of this study was to explore for any association between genetic variants of previously established 36 T2DM-associated SNPs and altered serum adipocytokine levels and metabolic syndrome phenotypes. Study consisted of 566 subjects (284 males and 282 females) of whom 147 were T2DM patients and 419 healthy controls. Study subjects were genotyped for 36 T2DM-linked single nucleotide polymorphisms (SNPs) using the KASPar SNP Genotyping System and grouped into different genotypes for each SNP. Various anthropometric and biochemical parameters were measured following standard procedures. The mean values of serum levels of individual adipocytokines and the presence/absence of metabolic syndrome phenotypes corresponding to various genotypes were compared by determining the odds ratios. Genotypic variants of five and seven of the 36 T2DM-related SNPs were significantly associated with altered serum levels of adiponectin and aPAI, respectively. Six variants of the 36 SNPs were associated with metabolic syndrome manifestations. This study identified positive associations between genotypic variants of five and seven of the 36 T2DM related SNPs and altered serum levels of adiponectin and aPAI, respectively. Six of 36 SNPs were also associated with metabolic syndrome in the studied population. The relation between specific SNPs and individual phenotypic traits may be useful in explaining the causal mechanisms of hereditary component of T2DM.
authors.affiliation: Department of Biochemistry, Biomarkers Research Program, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
authors.forename: Nasser M
authors.initials: NM
authors.lastname: Al-Daghri
authors.affiliation: Department of Biochemistry, Biomarkers Research Program, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
authors.forename: Omar S
authors.initials: OS
authors.lastname: Al-Attas
authors.affiliation: Department of Biochemistry, Biomarkers Research Program, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
authors.forename: Soundararajan
authors.initials: S
authors.lastname: Krishnaswamy
authors.affiliation: Department of Biochemistry, Biomarkers Research Program, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
authors.forename: Abdul Khader
authors.initials: AK
authors.lastname: Mohammed
authors.affiliation: School of Biological Sciences, University of Southampton Southampton SO17 1BJ, UK.
authors.forename: Amal M
authors.initials: AM
authors.lastname: Alenad
authors.affiliation: First Department of Pediatrics, Athens University Medical School Athens, Greece.
authors.forename: George P
authors.initials: GP
authors.lastname: Chrousos
authors.affiliation: Department of Biochemistry, Biomarkers Research Program, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
authors.forename: Majed S
authors.initials: MS
authors.lastname: Alokail
keywords: Adiponectin; GWAS; SNP; Type 2 Diabetes Mellitus; aPAI; insulin resistance; metabolic syndrome; obesity; resistin