title: Sesamin Ameliorates Advanced Glycation End Products-Induced Pancreatic β-Cell Dysfunction and Apoptosis.
abstract: Advanced glycation end products (AGEs), the direct modulators of β-cells, have been shown to cause insulin-producing β-cell dysfunction and apoptosis through increase of intracellular reactive oxygen species (ROS) production. Sesamin has been demonstrated to possess antioxidative activity. This study was designed to investigate whether sesamin protects against AGEs-evoked β-cell damage via its antioxidant property. The effects of sesamin were examined in C57BL/6J mice and MIN6 cell line. In in vivo studies, mice were intraperitoneally injected with AGEs (120 mg/kg) and orally treated with sesamin (160 mg/kg) for four weeks. Intraperitoneal glucose tolerance and insulin releasing tests were performed. Insulin content, ROS generation and β-cell apoptosis in pancreatic islets were also measured. In in vitro studies, MIN6 cells were pretreated with sesamin (50 or 100 μM) and then exposed to AGEs (200 mg/L) for 24 h. Insulin secretion, β-cell death, ROS production as well as expression and activity of NADPH oxidase were determined. Sesamin treatment obviously ameliorated AGE-induced β-cell dysfunction and apoptosis both in vivo and in vitro. These effects were associated with decreased ROS production, down-regulated expression of p67(phox) and p22(phox), and reduced NADPH oxidase activity. These results suggest that sesamin protects β-cells from damage caused by AGEs through suppressing NADPH oxidase-mediated oxidative stress.
authors.affiliation: Department of Endocrinology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. wnmcyaolikx@sina.com.|Department of Pharmacology, Wannan Medical College, Wuhu 241002, China. wnmcyaolikx@sina.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241002, China. guodong201@163.com.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. mmz666@163.com.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Ma
authors.affiliation: Department of Endocrinology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. luckyruyuan@163.com.
authors.forename: Ru-Yuan
authors.initials: RY
authors.lastname: Deng
authors.affiliation: Department of Pharmacology, Wannan Medical College, Wuhu 241002, China. wy_glq@163.com.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Guo
authors.affiliation: Department of Pharmacology, Wannan Medical College, Wuhu 241002, China. junxiuzhang@qq.com.
authors.forename: Jun-Xiu
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Wannan Medical College, Wuhu 241002, China. wnmcyaoli@sina.com.
authors.forename: Jie-Ren
authors.initials: JR
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. suqingxinhua@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Su
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004149:Dioxoles / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004149:Dioxoles; D017127:Glycation End Products, Advanced; D007328:Insulin; D017705:Lignans; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases; C054125:sesamin
keywords: MIN6 cell; advanced glycation end products; apoptosis; reactive oxygen  species; sesamin