title: Design and Generation of Gene-Targeting Vectors.
abstract: This unit provides an overview of the major types of mutant alleles that can be generated by gene targeting in ES cells. It presents the growing public resources of premade gene targeting vectors, modified ES cells, and mutant mice. General guidelines for the design of targeting vectors are followed by protocols for the construction of vectors to generate knockout (KO), conditional KO, and subtle mutant alleles. Curr. Protoc. Mouse Biol. 1:199-211. © 2011 by John Wiley & Sons, Inc.
authors.affiliation: German Research Center for Environmental Health, Munich, Germany.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Wefers
authors.affiliation: German Research Center for Environmental Health, Munich, Germany.|Technical University Munich, Munich, Germany.|Max-Planck-Institute of Psychiatry, Munich, Germany.|Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE), Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.affiliation: German Research Center for Environmental Health, Munich, Germany.|Technical University Munich, Munich, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Kühn
keywords: EUCOMM/KOMP; conditional KO mice; gene targeting; knockout mice; targeting vector design