title: Comparative proteomic analysis of the silkworm middle silk gland reveals the importance of ribosome biogenesis in silk protein production.
abstract: The silkworm middle silk gland (MSG) is the sericin synthesis and secretion unique sub-organ. The molecular mechanisms of regulating MSG protein synthesis are largely unknown. Here, we performed shotgun proteomic analysis on the three MSG subsections: the anterior (MSG-A), middle (MSG-M), and posterior (MSG-P) regions. The results showed that more strongly expressed proteins in the MSG-A were involved in multiple processes, such as silk gland development and silk protein protection. The proteins that were highly expressed in the MSG-M were enriched in the ribosome pathway. MSG-P proteins with stronger expression were mainly involved in the oxidative phosphorylation and citrate cycle pathways. These results suggest that the MSG-M is the most active region in the sericin synthesis. Furthermore, comparing the proteome of the MSG with the posterior silk gland (PSG) revealed that the specific and highly expressed proteins in the MSG were primarily involved in the ribosome and aminoacyl-tRNA biosynthesis pathways. These results indicate that silk protein synthesis is much more active as a result of the enhancement of translation-related pathways in the MSG. These results also suggest that enhancing ribosome biogenesis is important to the efficient synthesis of silk proteins.
authors.affiliation: Institute of Developmental and Regenerative Biology, Hangzhou Normal University, Hangzhou 310036, PR China; College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Li
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Lu-peng
authors.initials: LP
authors.lastname: Ye
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Jia-qian
authors.initials: JQ
authors.lastname: Che
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Zheng-ying
authors.initials: ZY
authors.lastname: You
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Ki-chan
authors.initials: KC
authors.lastname: Yun
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Wang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China. Electronic address: bxzhong@zju.edu.cn.
authors.forename: Bo-xiong
authors.initials: BX
authors.lastname: Zhong
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D005088:Exocrine Glands / Q000473:pathology*; D040901:Proteomics; D012270:Ribosomes / Q000378:metabolism*; D047011:Silk / Q000378:metabolism*
chemical_list: D047011:Silk
keywords: Bombyx mori; Label-free; Middle silk gland; Ribosome biogenesis; Shotgun proteomics; Silk protein synthesis