title: Radial glial cell-specific ablation in the adult Zebrafish brain.
abstract: The zebrafish brain can continue to produce new neurons in widespread neurogenic brain regions throughout life. In contrast, neurogenesis in the adult mammalian brain is restricted to the subventricular zone (SVZ) and dentate gyrus (DG). In neurogenic regions in the adult brain, radial glial cells (RGCs) are considered to function as neural stem cells (NSCs). We generated a Tg(gfap:Gal4FF) transgenic zebrafish line, which enabled us to express specific genes in RGCs. To study the function of RGCs in neurogenesis in the adult zebrafish brain, we also generated a Tg(gfap: Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, which allowed us to induce cell death exclusively within RGCs upon addition of metronidazole (Mtz) to the media. RGCs expressing nitroreductase were specifically ablated by the Mtz treatment, decreasing the number of proliferative RGCs. Using the Tg(gfap:Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, we found that RGCs were specifically ablated in the adult zebrafish telencephalon. The Tg(gfap:Gal4FF) line could be useful to study the function of RGCs.
authors.affiliation: Department of Life Science and Medical Bioscience, Laboratory for Molecular Brain Science, Waseda University, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shimizu
authors.affiliation: Department of Life Science and Medical Bioscience, Laboratory for Molecular Brain Science, Waseda University, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Life Science and Medical Bioscience, Laboratory for Molecular Brain Science, Waseda University, Tokyo, Japan.
authors.forename: Hideomi
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Life Science and Medical Bioscience, Laboratory for Molecular Brain Science, Waseda University, Tokyo, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Ohshima
mesh_terms: D055011:Ablation Techniques / Q000379:methods; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism; D063928:Ependymoglial Cells / Q000166:cytology* / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D015027:Zebrafish
chemical_list: D005904:Glial Fibrillary Acidic Protein
keywords: Gal4-UAS; adult neurogenesis; cell ablation; transgenic; zebrafish