title: Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins.
abstract: BACKGROUND
  Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human.
  
  
  OBJECTIVE
  The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (TGF-β).
  
  
  METHODS
  Toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and E/SA was harvested and used in animal immunization with and without adjuvant. Serum levels of anti-E/SA antibodies and TGF-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using ELISA technique. The measurements were statistically analyzed.
  
  
  RESULTS
  Our results showed that the serum levels of anti-E/SA immunoglobulins significantly increased in all of the immunized groups. The differences of the serum levels of TGF-β between the groups were statistically significant at days 28 and 56 after immunization with E/SA.
  
  
  CONCLUSIONS
  Based on our study, in vivo prepared E/SA may be considered as a good candidate for animal immunization.
authors.affiliation: Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Abdollahi
authors.affiliation: Physiology and Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Fateme
authors.initials: F
authors.lastname: Ayoobi
authors.affiliation: Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Khorramdelazad
authors.affiliation: Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Nasiri Ahmadabadi
authors.affiliation: Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Mohammadtaghi
authors.initials: M
authors.lastname: Rezayati
authors.affiliation: Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kazemi Arababadi
authors.affiliation: Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Zare-Bidaki
keywords: Excretory-Secretory Antigen; Immunization; Toxoplasma; Transforming Growth Factor-beta