title: The relationship between telomere length and clinicopathologic characteristics in colorectal cancers among Tunisian patients.
abstract: Alterations in telomere dynamics have emerged as having a causative role in carcinogenesis. Both the telomere attrition contribute to tumor initiation via increasing chromosomal instability and that the telomere elongation induces cell immortalization and leads to tumor progression. The objectives of this study are to investigate the dynamics of telomere length in colorectal cancer (CRC) and the clinicopathological parameters implicated. We measured the relative telomere length (RTL) in cancerous tissues and in corresponding peripheral blood leukocytes (PBL) using quantitative PCR (Q-PCR) from 94 patients with CRC. Telomere length correlated significantly in cancer tissues and corresponding PBL (r = 0.705). Overall, cancer tissue had shorter telomeres than PBL (p = 0.033). In both cancer tissue and PBL, the RTL was significantly correlated with age groups (p = 0.008 and p = 0.012, respectively). The RTL in cancer tissue was significantly longer in rectal tumors (p = 0.04) and in the late stage of tumors (p = 0.01). In PBL, the RTL was significantly correlated with the macroscopic aspect of tumors (p = 0.02). In addition, the telomere-length ratio of cancer to corresponding PBL increased significantly with late-stage groups. Shortening of the telomere was detected in 44.7%, elongation in 36.2%, and telomeres were unchanged in 19.1% of 94 tumors. Telomere shortening occurred more frequently in the early stage of tumors (p = 0.01). This study suggests that the telomere length in PBL is affected by the macroscopic aspect of tumors and that telomere length in cancer tissues is a marker for progression of CRC and depends on tumor-origin site.
authors.affiliation: Human Genetics Laboratory, Faculty of Medicine of Tunis, 15 rue Djebel Lakdhar La Rabta, Tunis, 1007, Tunisia.
authors.forename: Raja
authors.initials: R
authors.lastname: Mzahma
authors.affiliation: Human Genetics Laboratory, Faculty of Medicine of Tunis, 15 rue Djebel Lakdhar La Rabta, Tunis, 1007, Tunisia.
authors.forename: Maher
authors.initials: M
authors.lastname: Kharrat
authors.affiliation: Department of General Surgery A, La Rabta Hospital, Tunis, 1007, Tunisia.
authors.forename: Fadhel
authors.initials: F
authors.lastname: Fetiriche
authors.affiliation: Department of General Surgery B, Charles Nicole Hospital, Boulevard 9 Avril, Tunis, 1007, Tunisia.
authors.lastname: Bouasker
authors.affiliation: Department of General Surgery A, Charles Nicole Hospital, Boulevard 9 Avril, Tunis, 1007, Tunisia.
authors.forename: Mounir
authors.initials: M
authors.lastname: Ben Moussa
authors.affiliation: Department of General Surgery A, La Rabta Hospital, Tunis, 1007, Tunisia.
authors.forename: Zoubeir
authors.initials: Z
authors.lastname: Ben Safta
authors.affiliation: Department of General Surgery B, Charles Nicole Hospital, Boulevard 9 Avril, Tunis, 1007, Tunisia.
authors.forename: Chadli
authors.initials: C
authors.lastname: Dziri
authors.affiliation: Department of General Surgery A, Charles Nicole Hospital, Boulevard 9 Avril, Tunis, 1007, Tunisia.
authors.forename: AbdelJelil
authors.initials: A
authors.lastname: Zaouche
authors.affiliation: Human Genetics Laboratory, Faculty of Medicine of Tunis, 15 rue Djebel Lakdhar La Rabta, Tunis, 1007, Tunisia. habiba.chaabouni@rns.tn.|Human Genetics Department, Charles Nicole Hospital, Boulevard 9 Avril, Tunis, 1007, Tunisia. habiba.chaabouni@rns.tn.
authors.forename: Habiba
authors.initials: H
authors.lastname: Chaabouni-Bouhamed
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D043171:Chromosomal Instability / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D019098:Telomerase / Q000235:genetics; D016615:Telomere / Q000235:genetics; D059505:Telomere Homeostasis / Q000235:genetics*; D014416:Tunisia
chemical_list: D019098:Telomerase
keywords: Clinicopathologic characteristics; Colorectal cancer; Telomere length