title: Honey feeding protects kidney against cisplatin nephrotoxicity through suppression of inflammation.
abstract: Cisplatin is a highly effective chemotherapeutic drug used to treat a wide variety of solid tumors. However, its use was limited due its dose-limiting toxicity to the kidney. Currently, there are no therapies available to treat or prevent cisplatin nephrotoxicity. Honey is a naturally occurring complex liquid and widely used in traditional Ayurvedic medicine to treat many illnesses. However, its effect on cisplatin nephrotoxicity is unknown. To determine the role of honey in cisplatin nephrotoxicity, animals were pretreated orally for a week and then cisplatin was administered. Honey feeding was continued for another 3 days. Our results show that animals with cisplatin-induced kidney dysfunction, as determined by increased serum creatinine, which received honey feeding had less kidney dysfunction. Improved kidney function was associated with better preservation of kidney morphology in honey-treated group as compared to the cisplatin alone-treated group. Interestingly, honey feeding significantly reduced cisplatin-induced tubular epithelial cell death, immune infiltration into the kidney as well as cytokine and chemokine expression and excretion as compared to cisplatin treated animals. Western blot analysis shows that cisplatin-induced increase in phosphorylation of NFkB was completely suppressed with honey feeding. In conclusion, honey feeding protects the kidney against cisplatin nephrotoxicity through suppression of inflammation and NFkB activation.
authors.affiliation: Department of Medicine and Vascular Biology Center, Georgia Regents University, Augusta, GA, USA.|Department of Pathology, Suez Canal University, Suez Canal, Egypt.
authors.forename: Rania
authors.initials: R
authors.lastname: Hamad
authors.affiliation: Department of Medicine and Vascular Biology Center, Georgia Regents University, Augusta, GA, USA.
authors.forename: Calpurnia
authors.initials: C
authors.lastname: Jayakumar
authors.affiliation: Department of Medicine and Vascular Biology Center, Georgia Regents University, Augusta, GA, USA.
authors.forename: Punithavathi
authors.initials: P
authors.lastname: Ranganathan
authors.affiliation: Department of Medicine and Vascular Biology Center, Georgia Regents University, Augusta, GA, USA.
authors.forename: Riyaz
authors.initials: R
authors.lastname: Mohamed
authors.affiliation: Department of Pathology, Suez Canal University, Suez Canal, Egypt.
authors.forename: Mahmoud M I
authors.initials: MM
authors.lastname: El-Hamamy
authors.affiliation: Department of Pathology, Suez Canal University, Suez Canal, Egypt.
authors.forename: Amina A
authors.initials: AA
authors.lastname: Dessouki
authors.affiliation: Department of Pathology, Suez Canal University, Suez Canal, Egypt.
authors.forename: Abdelazim
authors.initials: A
authors.lastname: Ibrahim
authors.affiliation: Department of Medicine and Vascular Biology Center, Georgia Regents University, Augusta, GA, USA.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Ramesh
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D019610:Cytoprotection / Q000187:drug effects*; D006722:Honey; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D002945:Cisplatin
keywords: NFkB; acute kidney injury; cisplatin; honey; inflammation; nephrotoxicity