title: Analysis of Ergot Alkaloids.
abstract: The principles and application of established and newer methods for the quantitative and semi-quantitative determination of ergot alkaloids in food, feed, plant materials and animal tissues are reviewed. The techniques of sampling, extraction, clean-up, detection, quantification and validation are described. The major procedures for ergot alkaloid analysis comprise liquid chromatography with tandem mass spectrometry (LC-MS/MS) and liquid chromatography with fluorescence detection (LC-FLD). Other methods based on immunoassays are under development and variations of these and minor techniques are available for specific purposes.
authors.affiliation: Fera Science Ltd., Sand Hutton, North Yorks YO41 1LZ, UK. colin.crews@fera.co.uk.
authors.forename: Colin
authors.initials: C
authors.lastname: Crews
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004876:Ergot Alkaloids / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D007118:Immunoassay; D053719:Tandem Mass Spectrometry
chemical_list: D004876:Ergot Alkaloids
keywords: LSD; ergot; ergotamine; ergovaline; lysergic acid; mycotoxins