title: Chronic inflammatory pain upregulates expression of P2Y2 receptor in small-diameter sensory neurons.
abstract: Roles of ionotropic purinergic (P2X) receptors in chronic pain have been intensively investigated. However, the contribution of metabotropic purinergic (P2Y) receptors to pathological pain is controversial. In the present study, using single cell RT-PCR (reverse transcription-polymerase chain reaction) and single cell nested-PCR techniques, we examined the expression of P2X(2), P2X(3), P2Y(1) and P2Y(2) mRNA transcripts in retrogradely labeled cutaneous sensory neurons from mouse lumber dorsal root ganglia (DRGs) following peripheral inflammation. The percentage of cutaneous sensory neurons expressing P2Y(2) mRNA transcripts increased after complete Freund's adjuvant (CFA) treatment. Particularly, the P2Y(2) mRNA transcripts were more frequently detected in small-diameter cutaneous neurons from CFA-treated mice than those from control mice. Coexpression of P2Y(2) and P2X (P2X(2) or P2X(3)) mRNAs was more frequently observed in cutaneous sensory neurons from CFA-treated mice relative to controls. Pain behavioral tests showed that the blockade of P2Y receptors by suramin attenuated mechanical allodynia evoked either by CFA or uridine triphosphate (UTP), an endogenous P2Y(2) and P2Y(4) agonist. These results suggest that chronic inflammatory pain enhances expression of P2Y(2) receptor in peripheral sensory neurons that innervate the injured tissue and the activation of P2Y receptors contributes to mechanical allodynia following inflammation.
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Chenli
authors.initials: C
authors.lastname: Li
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Jiangyuan
authors.initials: J
authors.lastname: Yu
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Xu
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wei
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China.
authors.forename: Qinwen
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. chenxiaowei@nbu.edu.cn.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism / Q000523:psychology; D001522:Behavior, Animal; D059350:Chronic Pain / Q000209:etiology / Q000378:metabolism* / Q000523:psychology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism / Q000523:psychology; D007249:Inflammation / Q000150:complications / Q000378:metabolism* / Q000523:psychology; D051379:Mice; D008813:Mice, Inbred ICR; D059225:Nociception; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D058470:Receptors, Purinergic P2Y / Q000096:biosynthesis / Q000235:genetics; D058473:Receptors, Purinergic P2Y2 / Q000096:biosynthesis* / Q000235:genetics; D011984:Sensory Receptor Cells / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000294:innervation; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D058470:Receptors, Purinergic P2Y; D058473:Receptors, Purinergic P2Y2
keywords: Inflammatory pain; P2Y; Purinergic receptor; Single cell PCR