title: Optimization of extraction of high purity all-trans-lycopene from tomato pulp waste.
abstract: The aim of this work was to optimize the extraction of pure all-trans-lycopene from the pulp fractions of tomato processing waste. A full factorial design (FFD) consisting of four independent variables including extraction temperature (30-50 °C), time (1-60 min), percentage of acetone in n-hexane (25-75%, v/v) and solvent volume (10-30 ml) was used to investigate the effects of process variables on the extraction. The absolute amount of lycopene present in the pulp waste was found to be 0.038 mg/g. The optimal conditions for extraction were as follows: extraction temperature 20 °C, time 40 min, a solvent composition of 25% acetone in n-hexane (v/v) and solvent volume 40 ml. Under these conditions, the maximal recovery of lycopene was 94.7%. The HPLC-DAD analysis demonstrated that, lycopene was obtained in the all-trans-configuration at a very high purity grade of 98.3% while the amount of cis-isomers and other carotenoids were limited.
authors.affiliation: University of Camerino, School of Science & Technology, Section Chemistry, Via S. Agostino 1, 62032 Camerino, Italy.
authors.forename: Mahesha M
authors.initials: MM
authors.lastname: Poojary
authors.affiliation: University of Camerino, School of Science & Technology, Section Chemistry, Via S. Agostino 1, 62032 Camerino, Italy. Electronic address: paolo.passamonti@unicam.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Passamonti
mesh_terms: D002338:Carotenoids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019649:Food Industry / Q000379:methods*; D007220:Industrial Waste / Q000032:analysis*; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D007220:Industrial Waste; D002338:Carotenoids; D000077276:Lycopene
keywords: All-trans-lycopene; Factorial design; Lycopene (PubChem CID: 446925); Lycopene extraction; Optimization; Tomato processing waste