title: Novel ageing-biomarker discovery using data-intensive technologies.
abstract: Ageing is accompanied by many visible characteristics. Other biological and physiological markers are also well-described e.g. loss of circulating sex hormones and increased inflammatory cytokines. Biomarkers for healthy ageing studies are presently predicated on existing knowledge of ageing traits. The increasing availability of data-intensive methods enables deep-analysis of biological samples for novel biomarkers. We have adopted two discrete approaches in MARK-AGE Work Package 7 for biomarker discovery; (1) microarray analyses and/or proteomics in cell systems e.g. endothelial progenitor cells or T cell ageing including a stress model; and (2) investigation of cellular material and plasma directly from tightly-defined proband subsets of different ages using proteomic, transcriptomic and miR array. The first approach provided longitudinal insight into endothelial progenitor and T cell ageing. This review describes the strategy and use of hypothesis-free, data-intensive approaches to explore cellular proteins, miR, mRNA and plasma proteins as healthy ageing biomarkers, using ageing models and directly within samples from adults of different ages. It considers the challenges associated with integrating multiple models and pilot studies as rational biomarkers for a large cohort study. From this approach, a number of high-throughput methods were developed to evaluate novel, putative biomarkers of ageing in the MARK-AGE cohort.
authors.affiliation: Aston Research Centre for Healthy Ageing and Life & Health Sciences, Aston University, Birmingham, B4 7ET, UK. Electronic address: h.r.griffiths@aston.ac.uk.
authors.forename: H R
authors.initials: HR
authors.lastname: Griffiths
authors.affiliation: Aston Research Centre for Healthy Ageing and Life & Health Sciences, Aston University, Birmingham, B4 7ET, UK.
authors.forename: E M
authors.initials: EM
authors.lastname: Augustyniak
authors.affiliation: Aston Research Centre for Healthy Ageing and Life & Health Sciences, Aston University, Birmingham, B4 7ET, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Bennett
authors.affiliation: NARILIS-URBC, University of Namur, Namur, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Debacq-Chainiaux
authors.affiliation: Aston Research Centre for Healthy Ageing and Life & Health Sciences, Aston University, Birmingham, B4 7ET, UK.
authors.forename: C R
authors.initials: CR
authors.lastname: Dunston
authors.affiliation: Aarhus University Department of Engineering Gustav Wieds Vej 10, 8000 Aarhus, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Kristensen
authors.affiliation: Aarhus University Department of Engineering Gustav Wieds Vej 10, 8000 Aarhus, Denmark.
authors.forename: C J
authors.initials: CJ
authors.lastname: Melchjorsen
authors.affiliation: Martin Luther University of Halle-Wittenberg, Clinic for Cardiac und Thoracic Surgery, Germany.
authors.forename: Santos A
authors.initials: SA
authors.lastname: Navarrete
authors.affiliation: Martin Luther University of Halle-Wittenberg, Clinic for Cardiac und Thoracic Surgery, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Simm
authors.affiliation: NARILIS-URBC, University of Namur, Namur, Belgium.
authors.forename: O
authors.initials: O
authors.lastname: Toussaint
mesh_terms: D000328:Adult; D000375:Aging / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D057225:Data Mining / Q000379:methods*; D006801:Humans; D012984:Software
chemical_list: D015415:Biomarkers
keywords: Biomarker discovery; Endothelial progenitor cells; Hyperoxia; Proteomics; miR array