title: Surface-modified cobalt oxide nanoparticles: new opportunities for anti-cancer drug development.
abstract: The development of smart nanoparticles that can exhibit the anti-cancer activity, introduces better efficacy and lower toxicity for treatment. The present study was aimed to evaluate the anti-cancer activity of surface functionalized CoO nanoparticles against Jurkat (T-cell lymphoma) and KB (oral carcinoma) cell lines. The nano-sized cobalt oxide nanoparticles (CoO) was prepared by thermal decomposition method followed by surface modification using phosphonomethyl iminodiacetic acid (PMIDA). The PMIDA-coated CoO nanoparticle was characterized by X-ray diffraction, dynamic light scattering, and transmission electron microscopy; and the conjugation was analyzed by Fourier transform infrared spectroscopy. The resultant nanoparticles with an average size less than 100 nm measured by dynamic light scattering and transmission electron microscopy. Cytotoxicity study, flow cytometric analysis and scanning electron micrographs have been revealed that PMIDA-coated nanoparticles significantly enhances the cellular uptake of the nanoparticle and thus facilitates apoptosis of cancer cell (Jurkat and KB). For the application of PMIDA-coated CoO nanoparticles in the medical field, doxorubicin, a potent anti-cancer drug, has been used in similar fashion in this experimental design and all these effects or patterns were observed.
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102 West Bengal India.
authors.forename: S
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102 West Bengal India.
authors.forename: S P
authors.initials: SP
authors.lastname: Chakraborty
authors.affiliation: Nano materials Laboratory, Department of Chemistry, Indian Institute of Technology, Kharagpur, West Bengal India.
authors.forename: D
authors.initials: D
authors.lastname: Laha
authors.affiliation: Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, 700 026 India.
authors.forename: R
authors.initials: R
authors.lastname: Baral
authors.affiliation: Nano materials Laboratory, Department of Chemistry, Indian Institute of Technology, Kharagpur, West Bengal India.
authors.forename: P
authors.initials: P
authors.lastname: Pramanik
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102 West Bengal India.
authors.forename: S
authors.initials: S
authors.lastname: Roy
keywords: Anti-cancer drug; Cancer cell line; Cellular apoptosis; Cobalt oxide nanoparticles; Cytotoxicity; SEM micrographs