title: Metabolic engineering of Escherichia coli for the production of 3-aminopropionic acid.
abstract: A novel metabolic pathway was designed for the production of 3-aminopropionic acid (3-AP), an important platform chemical for manufacturing acrylamide and acrylonitrile. Using a fumaric acid producing Escherichia coli strain as a host, the Corynebacterium glutamicum panD gene (encoding L-aspartate-α-decarboxylase) was overexpressed and the native promoter of the aspA gene was replaced with the strong trc promoter, which allowed aspartic acid production through the aspartase-catalyzed reaction. Additional overexpression of aspA and ppc genes, and supplementation of ammonium sulfate in the medium allowed production of 3.49 g/L 3-AP. The 3-AP titer was further increased to 3.94 g/L by optimizing the expression level of PPC using synthetic promoters and RBS sequences. Finally, native promoter of the acs gene was replaced with strong trc promoter to reduce acetic acid accumulation. Fed-batch culture of the final strain allowed production of 32.3 g/L 3-AP in 39 h.
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea; BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Song
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea; BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Joungmin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea; BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Yoo-Sung
authors.initials: YS
authors.lastname: Ko
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea; BioProcess Engineering Research Center, KAIST, Daejeon 305-701, Republic of Korea; BioInformatics Research Center, KAIST, Daejeon 305-701, Republic of Korea. Electronic address: leesy@kaist.ac.kr.
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D060847:Metabolic Engineering / Q000379:methods*; D015091:beta-Alanine / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D015091:beta-Alanine
keywords: 3-Aminopropionic acid; Escherichia coli; Fumaric acid; Metabolic engineering; β-Alanine