title: Dimethyl sulfoxide inactivates the anticancer effect of cisplatin against human myelogenous leukemia cell lines in in vitro assays.
abstract: OBJECTIVE
  To investigate the effect of DMSO on cisplatin induced cytotoxicity (invitro) against K562 (Human mylogenous leukemia) cell line and to study the cisplatin-DMSO adduct formation using UV-spectrophotometer.
  
  
  METHODS
  Effect of DMSO on the cytotoxicity of cisplatin was studied in K562 (Chronic mylogenous leukemia) cell line by MTT assay. Cisplatin-DMSO adduct formation was studied by continuously monitoring the increase in absorption peaks for 30 minutes using UV-spectrophotometer.
  
  
  RESULTS
  0.1-0.3% DMSO markedly reduced the cytotoxic activity of cisplatin in K562 cells. Cisplatin-DMSO adduct formation was detected using UV-spectrophotometer. Continuous increase in UV absorbance between 250nm-290nm was observed when cisplatin (0.5mg/ml) and DMSO (10%) were mixed.
  
  
  CONCLUSIONS
  Present study revealed that DMSO inactivates the cytotoxicity of cisplatin. Cisplatin-DMSO mixture showed increased absorbance at 250-290nm. Therefore, using DMSO in invitro assays might result in misinterpretation of actual efficacy of drugs.
authors.affiliation: Department of Biotechnology, University of Calicut, Malappuram, Kerala, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Raghavan
authors.affiliation: Department of Biotechnology, University of Calicut, Malappuram, Kerala, India.
authors.forename: Sanith
authors.initials: S
authors.lastname: Cheriyamundath
authors.affiliation: Department of Biotechnology, University of Calicut, Malappuram, Kerala, India.
authors.forename: Joseph
authors.initials: J
authors.lastname: Madassery
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*
chemical_list: D002945:Cisplatin; D004121:Dimethyl Sulfoxide
keywords: Cisplatin; DMSO; K562 cell line