title: FoxM1 influences mouse hepatocellular carcinoma metastasis in vitro.
abstract: Lymph node metastasis is recognized as an important mode of liver cancer metastasis. Two hepatocarcinoma cell lines, Hca-F get high (75%) and Hca-P get low (25%) incidences of lymph node metastasis. Forkhead box M1 (FoxM1) is described as a major oncogenic transcription factor in tumor initiation, promotion, and progression. Ezrin is linked to aggressive tumor behavior by involving all stages of tumor metastasis. We firstly compared the expression of FoxM1 and Ezrin between two cells. Then we transiently transfected Hca-P cells with over-FoxM1 plasmid and Hca-F cells with sh-FoxM1 plasmid. We found that both FoxM1 and Ezrin expressed higher in Hca-F than Hca-P. We successfully down-regulated or up-regulated FoxM1 expression in Hca-F or Hca-P cells. And we found that FoxM1 had correlation with proliferation, invasion and migration in mouse hepatocellular carcinoma cell lines.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Dalian Medical University Dalian 116011, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University Dalian 116044, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Intervention, Dalian Sixth People's Hospital 116037, China.
authors.forename: Benke
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Dalian Medical University 116011, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Dalian Medical University Dalian 116011, China.
authors.forename: Aman
authors.initials: A
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Dalian Medical University Dalian 116011, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Cui
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000378:metabolism; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D000099149:Ezrin; C491524:Foxm1 protein, mouse
keywords: FoxM1; ezrin; hepatocarcinoma; lymphatic metastasis