title: Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles.
abstract: Lineage tracing using Cre/lox technology has become a well-established technique to study the contribution of different (stem) cell populations to organ development and function. When used in the mammary gland, it forms a valuable addition to the already existing experimental toolbox and an important alternative to other readouts measuring stem cell potential, such as the fat pad transplantation assay.Here I describe how to set up and analyze an in vivo lineage tracing experiment using tamoxifen-inducible Cre/lox technology, highlighting the specific challenges that the investigator faces when employing this method and interpreting the results in the mammary gland.
authors.affiliation: Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands, r.vanamerongen@uva.nl.
authors.forename: Renée
authors.initials: R
authors.lastname: van Amerongen
mesh_terms: D000483:Alleles; D000818:Animals; D019070:Cell Lineage / Q000235:genetics*; D058948:Cell Tracking / Q000379:methods*; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D059765:Homologous Recombination; D008321:Mammary Glands, Animal / Q000166:cytology*; D051379:Mice; D018613:Microscopy, Confocal; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D013629:Tamoxifen
chemical_list: D013629:Tamoxifen