title: A label-free and self-assembled electrochemical biosensor for highly sensitive detection of cyclic diguanylate monophosphate (c-di-GMP) based on RNA riboswitch.
abstract: Cyclic diguanylate monophosphate (c-di-GMP) is an important second messenger that regulates a variety of complex physiological processes involved in motility, virulence, biofilm formation and cell cycle progression in several bacteria. Herein we report a simple label-free and self-assembled RNA riboswitch-based biosensor for sensitive and selective detection of c-di-GMP. The detectable concentration range of c-di-GMP is from 50 nM to 1 μM with a detection limit of 50 nM.
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China(1).
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China(1).
authors.forename: Fulin
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China(1).
authors.forename: Hongrui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China(1).
authors.forename: Yanke
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China(1). Electronic address: feiliu24@njau.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D058928:Riboswitch
chemical_list: D058928:Riboswitch; D006152:Cyclic GMP
keywords: Biosensor; Cyclic diguanylate monophosphate (c-di-GMP); Label-free; Riboswitch; Self-assembled