title: In Vitro Inhibition of 4-Nitroquinoline-1-Oxide Genotoxicity by Probiotic Lactobacillus rhamnosus IMC501.
abstract: Inhibition of 4-nitroquinoline-1-oxide (4-NQO) genotoxicity by a probiotic strain of Lactobacillus rhamnosus (IMC501) was assessed by the prokaryotic short-term bioassay SOSChromotest, using Escherichia coli PQ37 as the target organism. Results showed the ability of strain IMC501 to rapidly and markedly counteract, in vitro, the DNA damage originated by the considered genotoxin. The inhibition was associated with a spectroscopic hypsochromic shift of the original 4-NQO profile and progressive absorbance increase of a new peak. IR-Raman and GC-MS analyses confirmed the disappearance of 4-NQO after contact with the microorganism, showing also the absence of any genotoxic molecule potentially available for metabolic activation (i.e., 4-hydroxyaminoquinoline-1-oxide and 4-nitrosoquinoline-1-oxide). Furthermore, we have shown the presence of the phenyl-quinoline and its isomers as major non-genotoxic conversion products, which led to the hypothesis of a possible pattern of molecular transformation. These findings increase knowledge on lactobacilli physiology and contribute to the further consideration of antigenotoxicity as a nonconventional functional property of particular probiotic strains.
authors.affiliation: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, I-06122 Perugia, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bocci
authors.affiliation: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, I-06122 Perugia, Italy.
authors.forename: Bartolomeo
authors.initials: B
authors.lastname: Sebastiani
authors.affiliation: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, I-06122 Perugia, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Trotta
authors.affiliation: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, I-06122 Perugia, Italy.
authors.forename: Ermanno
authors.initials: E
authors.lastname: Federici
authors.affiliation: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, I-06122 Perugia, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cenci
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000378:metabolism* / Q000633:toxicity*; D001681:Biological Assay; D001711:Biotransformation; D004926:Escherichia coli / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity*; D019936:Probiotics / Q000378:metabolism*; D013014:SOS Response, Genetics; D013059:Spectrum Analysis, Raman
chemical_list: D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide
keywords: 4-nitroquinoline-1-oxide; Lactobacillus rhamnosus; Probiotics; antigenotoxicity