title: Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture.
abstract: Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.
authors.affiliation: †Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Célio Fernando F
authors.initials: CF
authors.lastname: Angolini
authors.affiliation: †Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Pedro Henrique
authors.initials: PH
authors.lastname: Vendramini
authors.affiliation: †Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Francisca D S
authors.initials: FD
authors.lastname: Araújo
authors.affiliation: ‡Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, USP, 05508-900 São Paulo, SP, Brazil.
authors.forename: Welington L
authors.initials: WL
authors.lastname: Araújo
authors.affiliation: §Department of Chemistry, Federal University of Minas Gerais, UFMG, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Rodinei
authors.initials: R
authors.lastname: Augusti
authors.affiliation: †Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Marcos N
authors.initials: MN
authors.lastname: Eberlin
authors.affiliation: †Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Luciana Gonzaga
authors.initials: LG
authors.lastname: de Oliveira
mesh_terms: D000362:Agar; D013058:Mass Spectrometry / Q000379:methods*; D013302:Streptomyces / Q000254:growth & development
chemical_list: D000362:Agar