title: Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions.
abstract: Overexpression of SPS in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions. Sucrose phosphate synthase (SPS; EC 2.3.1.14) is the key enzyme in the synthesis of sucrose in plants. The outcome of overexpression of SPS in different plants using transgenic approaches has been quite varied, but the general consensus is that increased SPS activity is associated with the production of new sinks and increased sink strength. In legumes, the root nodule is a strong C sink and in this study our objective was to see how increasing SPS activity in a legume would affect nodule number and function. Here we have transformed alfalfa (Medicago sativa, cv. Regen SY), with a maize SPS gene driven by the constitutive CaMV35S promoter. Our results showed that overexpression of SPS in alfalfa, is accompanied by an increase in nodule number and mass and an overall increase in nitrogenase activity at the whole plant level. The nodules exhibited an increase in the level of key enzymes contributing to N assimilation including glutamine synthetase and asparagine synthetase. Moreover, the stems of the transformants showed higher level of the transport amino acids, Asx, indicating increased export of N from the nodules. The transformants exhibited a dramatic increase in growth both of the shoots and roots, and earlier flowering time, leading to increased yields. Moreover, the transformants showed an increase in elemental N and protein content. The overall conclusion is that increased SPS activity improves the N status and plant performance, suggesting that the availability of more C in the form of sucrose enhances N acquisition and assimilation in the nodules.
authors.affiliation: Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, 88003, USA.
authors.forename: Sayed
authors.initials: S
authors.lastname: Gebril
authors.forename: Mark
authors.initials: M
authors.lastname: Seger
authors.forename: Fabiola Muro
authors.initials: FM
authors.lastname: Villanueva
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Ortega
authors.forename: Suman
authors.initials: S
authors.lastname: Bagga
authors.forename: Champa
authors.initials: C
authors.lastname: Sengupta-Gopalan
mesh_terms: D005964:Glucosyltransferases / Q000378:metabolism*; D000455:Medicago sativa / Q000201:enzymology* / Q000254:growth & development; D009586:Nitrogen Fixation; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase