title: Fish Oil-Rich Diet Promotes Hematopoiesis and Alters Hematopoietic Niche.
abstract: The self-renewal and differentiation of hematopoietic stem cells (HSCs) in bone marrow are essential to replenish all blood cell types, but how this process is influenced by diet remains largely unclear. Here we show that a diet rich in fish oils promotes self-renewal of HSCs and extramedullary hematopoiesis. Chronic intake of a fish oil-rich diet increases the abundance of HSCs, alters the hematopoietic microenvironment, and, intriguingly, induces the expression of matrix metalloproteinase 12 (MMP12) in the bone marrow. Pointing to a direct effect of fish oil on MMP12 expression, omega-3 polyunsaturated fatty acids induce the expression of MMP12 in a dose-dependent manner in bone marrow cells. Importantly, down-regulation of MMP12 activity using an MMP12-specific inhibitor attenuates diet-induced myelopoiesis in both bone marrow and spleen. Thus, a fish oil-rich diet promotes hematopoiesis in the bone marrow and spleen, in part via the activity of MMP12. Taken together, these data provide new insights into diet-mediated regulation of hematopoiesis.
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Li
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Lu
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Mu-tian
authors.initials: MT
authors.lastname: Han
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Miao-miao
authors.initials: MM
authors.lastname: Zhang
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Qi-xiang
authors.initials: QX
authors.lastname: Shao
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Hua-xi
authors.initials: HX
authors.lastname: Xu
authors.affiliation: Department of Immunology (S.X., M.Z., Q.S., H.X., L.Q.) and Institute of Clinic Laboratory Diagnosis (S.X., X.L., L.C., M.H., M.Z., Q.S., H.X.), School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; and Division of Nutritional Sciences (S.X., L.Q.), Cornell University, Ithaca, New York 14853.
authors.forename: Ling
authors.initials: L
authors.lastname: Qi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004032:Diet; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D006411:Hematopoiesis, Extramedullary / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D055153:Stem Cell Niche / Q000187:drug effects*
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils