title: Improving arachidonic acid fermentation by Mortierella alpina through multistage temperature and aeration rate control in bioreactor.
abstract: Effective production of arachidonic acid (ARA) using Mortierella alpina was conducted in a 30-L airlift bioreactor. Varying the aeration rate and temperature significantly influenced cell morphology, cell growth, and ARA production, while the optimal aeration rate and temperature for cell growth and product formation were quite different. As a result, a two-stage aeration rate control strategy was constructed based on monitoring of cell morphology and ARA production under various aeration rate control levels (0.6-1.8 vvm). Using this strategy, ARA yield reached 4.7 g/L, an increase of 38.2% compared with the control (constant aeration rate control at 1.0 vvm). Dynamic temperature-control strategy was implemented based on the fermentation performance at various temperatures (13-28°C), with ARA level in total cellular lipid increased by 37.1% comparing to a constant-temperature control (25°C). On that basis, the combinatorial fermentation strategy of two-stage aeration rate control and dynamic temperature control was applied and ARA production achieved the highest level of 5.8 g/L.
authors.affiliation: a Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Gao
authors.affiliation: a Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zheng
authors.affiliation: a Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: a Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Xiao-Bei
authors.initials: XB
authors.lastname: Zhan
authors.affiliation: a Key Laboratory of Industrial Biotechnology , Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Lin
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D020094:Mortierella / Q000378:metabolism*; D013696:Temperature
chemical_list: D016718:Arachidonic Acid
keywords: Aeration rate; Mortierella alpina; airlift bioreactor; arachidonic acid; cell morphology; fermentation temperature