title: Reconstructing spinal dura-like tissue using electrospun poly(lactide-co-glycolide) membranes and dermal fibroblasts to seamlessly repair spinal dural defects in goats.
abstract: Many neuro- and spinal surgeries involving access to the underlying nervous tissue will cause defect of spinal dural mater, further resulting in cerebrospinal fluid leakage. The current work was thus aimed to develop a package which included two layers of novel electrospun membranes, dermal fibroblasts and mussel adhesive protein for repairing spinal dural defect. The inner layer is electrospun fibrous poly(lactide-co-glycolide) membrane with oriented microstructure (O-poly(lactide-co-glycolide)), which was used as a substrate to anchor dermal fibroblasts as seed cells to reconstitute dura-like tissue via tissue engineering technique. The outer layer is chitosan-coated electrospun nonwoven poly(lactide-co-glycolide) membrane (poly(lactide-co-glycolide)-chitosan). During surgery, the inner reconstituted tissue layer was first used to directly cover dura defects, while the outer layer was placed onwards with its marginal area tightly immobilized to the surrounding normal spinal dura aided by mussel adhesive protein. Efficacy of the current design was verified in goats with spinal dural defects (0.6 cm × 0.5 cm) in lumbar. It was shown that seamless and quick sealing of the defect area with the implants was realized by mussel adhesive protein. Guided tissue growth and regeneration in the defects of goats were observed when they were repaired by the current package. Effective cerebrospinal fluid containment and anti-adhesion of the regenerated tissue to the surrounding tissue could be achieved in the current animal model. Hence, it could be ascertained that the current package could be a favorite choice for surgeries involving spinal dural defects.
authors.affiliation: Department of Orthopaedic Surgery, Chezhan Road, Huzhou, China.
authors.forename: Fengbin
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Orthopaedic Surgery, Chezhan Road, Huzhou, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: National Tissue Engineering Center of China, East Jiang Chuan Road, Shanghai, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yin
authors.affiliation: Department of Orthopaedic Surgery, Changzheng Hospital, Feng Yang Road, Shanghai, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Orthopaedic Surgery, Chezhan Road, Huzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Orthopaedic Surgery, Changzheng Hospital, Feng Yang Road, Shanghai, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
authors.affiliation: National Tissue Engineering Center of China, East Jiang Chuan Road, Shanghai, China chendyspine@sina.com.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Cao
authors.affiliation: National Tissue Engineering Center of China, East Jiang Chuan Road, Shanghai, China Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, Department of Product Engineering, School of Chemical Engineering, East China University of Science and Technology, Mei Long Road, Shanghai, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Cen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D004388:Dura Mater / Q000002:abnormalities*; D006041:Goats; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Spinal dura engineering; cerebrospinal fluid; chitosan; mussel adhesive protein; poly(lactide-co-glycolide)