title: Inhibition of discoidin domain receptor 2-mediated lung cancer cells progression by gold nanoparticle-aptamer-assisted delivery of peptides containing transmembrane-juxtamembrane 1/2 domain.
abstract: The delivery of biologically functional peptides into mammalian cells can be a direct and effective method for cancer therapy and treatment of other diseases. Discoidin domain receptor 2 (DDR2) is a collagen-induced receptor tyrosine kinase recently identified as a novel therapeutic target in lung cancer. In this study, we report that peptides containing the functional domain of DDR2 can be efficiently delivered into lung malignant cancer cells via a gold nanoparticle-DNA aptamer conjugate (AuNP-Apt)-based system. Peptide delivery resulted in the abrogation of DDR2 activation triggered by collagen. Moreover, the peptide delivered by the AuNP-Apt system inhibited cancer cell proliferation and invasion mediated by DDR2 activation. Thus, these results suggest that peptide loaded onto AuNP-Apt conjugates can be used for the development of peptide-based biomedical applications for the treatment of DDR2-positive cancer.
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Daehwan
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Yeom
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Boeun
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: jeehyeon@cau.ac.kr.
authors.forename: Jeehyeon
authors.initials: J
authors.lastname: Bae
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: sangmyung.rhee@cau.ac.kr.
authors.forename: Sangmyung
authors.initials: S
authors.lastname: Rhee
mesh_terms: D052157:Aptamers, Nucleotide; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D002462:Cell Membrane; D000070818:Discoidin Domain Receptors; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D053768:Metal Nanoparticles; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors* / Q000502:physiology; D011975:Receptors, Mitogen / Q000037:antagonists & inhibitors* / Q000502:physiology
chemical_list: D052157:Aptamers, Nucleotide; D010455:Peptides; D011975:Receptors, Mitogen; D006046:Gold; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases
keywords: DNA aptamer; Discoidin domain receptor 2; Gold nanoparticle; Juxtamembrane domain; Peptide delivery