title: Isolation and identification of an allelopathic substance from Hibiscus sabdariffa.
abstract: In this study, an allelopathic substance was isolated from an aqueous methanol extract of Hibiscus sabdariffa L. by column chromatography and reverse phase HPLC. The chemical structure of the substance was determined by 1H NMR spectroscopy and mass spectrometry as trimethyl allo-hydroxycitrate. Trimethyl allo-hydroxycitrate inhibited the growth of cress hypocotyls and roots at concentrations greater than 10 mM. The concentrations required for 50% growth inhibition of the hypocotyls and roots of cress were 20.3 and 14.4 mM, respectively. The inhibitory activity of trimethyl allo-hydroxycitrate suggests that the substance may act as an allelopathic substance of H. sabdariffa.
authors.forename: Prapaipit
authors.initials: P
authors.lastname: Suwitchayanon
authors.forename: Piyatida
authors.initials: P
authors.lastname: Pukclai
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohno
authors.forename: Kiyotake
authors.initials: K
authors.lastname: Suenaga
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kato-Noguchi
mesh_terms: D019607:Brassicaceae / Q000187:drug effects / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D031584:Hibiscus / Q000737:chemistry*; D010675:Pheromones / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D010675:Pheromones; D010936:Plant Extracts