title: Biodegradable Gelatin Microcarriers Facilitate Re-Epithelialization of Human Cutaneous Wounds - An In Vitro Study in Human Skin.
abstract: The possibility to use a suspended tridimensional matrix as scaffolding for re-epithelialization of in vitro cutaneous wounds was investigated with the aid of a human in vitro wound healing model based on viable full thickness skin. Macroporous gelatin microcarriers, CultiSpher-S, were applied to in vitro wounds and cultured for 21 days. Tissue sections showed incorporation of wound edge keratinocytes into the microcarriers and thicker neoepidermis in wounds treated with microcarriers. Thickness of the neoepidermis was measured digitally, using immunohistochemical staining of keratins as epithelial demarcation. Air-lifting of wounds enhanced stratification in control wounds as well as wounds with CultiSpher-S. Immunohistochemical staining revealed expression of keratin 5, keratin 10, and laminin 5 in the neoepidermal component. We conclude that the CultiSpher-S microcarriers can function as tissue guiding scaffold for re-epithelialization of cutaneous wounds.
authors.affiliation: Division of Experimental Plastic Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
authors.forename: Susanna
authors.initials: S
authors.lastname: Lönnqvist
authors.affiliation: Division of Experimental Plastic Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Center for Integrative Regenerative Medicine (IGEN), Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Rakar
authors.affiliation: Division of Experimental Plastic Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Briheim
authors.affiliation: Division of Experimental Plastic Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Hand and Plastic Surgery, Region Östergötland, Linköping, Sweden.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Kratz
mesh_terms: D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D017690:Cell Transplantation / Q000379:methods; D005780:Gelatin / Q000008:administration & dosage*; D006801:Humans; D007150:Immunohistochemistry; D053550:Keratin-10 / Q000096:biosynthesis; D053555:Keratin-5 / Q000096:biosynthesis; D015603:Keratinocytes / Q000166:cytology; D062326:Re-Epithelialization / Q000502:physiology*; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D046509:Tissue Culture Techniques; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology; D014947:Wounds and Injuries / Q000378:metabolism / Q000628:therapy*; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D005780:Gelatin; D053550:Keratin-10; D053555:Keratin-5; D000097794:Kalinin