title: Oxidative damage of DNA induced by X-irradiation decreases the uterine endometrial receptivity which involves mitochondrial and lysosomal dysfunction.
abstract: X irradiation may lead to female infertility and the mechanism is still not clear. After X irradiation exposure, significantly morphological changes and functional decline in endometrial epithelial cells were observed. The mitochondrial and lysosomal dysfunction and oxidative DNA damage were noticed after X irradiation. In addition, pretreatment with NAC, NH4Cl or Pep A reduced the X irradiation induced damages. These studies demonstrate that the oxidative DNA damage which involved dysfunctional lysosomal and mitochondrial contribute to X irradiation-induced impaired receptive state of uterine endometrium and proper protective reagents can be helpful in improving endometrial function.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian, PRC.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Surgery, First Affiliated Hospital of Dalian Medical University Dalian, PRC.
authors.forename: Jin-Xiao
authors.initials: JX
authors.lastname: Liang
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian, PRC.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian, PRC.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Public Health, Dalian Medical University Dalian, PRC.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Liu
authors.affiliation: Department of Dermatology, Northwestern University's Feinberg School of Medicine Chicago, IL 60611, USA.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering Dalian, PRC.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yan
keywords: DNA damage; X irradiation; endometrial receptivity; lysosomal membrane instability; mitochondrial dysfunction; oxidative stress