title: Decaffeinated green tea extract rich in epigallocatechin-3-gallate improves insulin resistance and metabolic profiles in normolipidic diet--but not high-fat diet-fed mice.
abstract: Supplementation with epigallocatechin-3-gallate (EGCG), which restores metabolic profiles, has been proposed as an option for preventing and treating obesity. We investigated whether decaffeinated green tea extract rich in EGCG, attenuates high-fat diet (HFD)-induced metabolic alterations in Swiss mice. The mice were maintained on either a control diet (CD) or HFD for 8 weeks and supplemented with either a placebo or EGCG (50mg/kg/day). Body weight, serum lipid profiles, cytokine protein expression, and content in epididymal (EPI) and retroperitoneal (RET) adipose tissues, and adipocyte area were measured. The body weights of HFD + placebo-fed mice were increased compared with those of HFD + EGCG-fed mice (28 and 21%, respectively), whereas the body weights of CD + EGCG-fed mice were decreased 16% compared with those of the CD + placebo group. Serum triglyceride levels were decreased 32% in the CD + EGCG group compared with the CD + placebo group. Compared with the CD + placebo group, increased phosphorylation of AMPK and hormone-sensitive lipase in EPI and RET, respectively, was found in the CD + EGCG group. Increased acetyl-CoA carboxylase phosphorylation was observed in both adipose tissues. In addition, TNF-α and IL-10 levels in EPI and adiponectin levels were higher in the CD + EGCG group than in the CD + placebo group. TNF-α levels were lower in the HFD + EGCG group than in the HFD + placebo group. Furthermore, the CD + EGCG group exhibited a lower adipocyte area than the CD + placebo group. These indicate that the effects of decaffeinated green tea extract on body mass may be related to the crosstalk between lipolytic and inflammatory pathways in normolipidic diet-fed mice but not in HFD-fed mice.
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Santana
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Santamarina
authors.affiliation: Laboratório de Movimento Humano, Universidade São Judas Tadeu, Sao Paulo, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Souza
authors.affiliation: Programa de Pós-Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Laís
authors.initials: L
authors.lastname: Mennitti
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Okuda
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Venancio
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, Sao Paulo, Brazil.
authors.forename: Marilia
authors.initials: M
authors.lastname: Seelaender
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Claudia Oller
authors.initials: CO
authors.lastname: do Nascimento
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Eliane
authors.initials: E
authors.lastname: Ribeiro
authors.affiliation: Exercise and Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Presidente Prudente, SP, Brazil.
authors.forename: Fabio
authors.initials: F
authors.lastname: Lira
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo, Sao Paulo, Brazil. Electronic address: lmoyama@gmail.com.
authors.forename: Lila
authors.initials: L
authors.lastname: Oyama
mesh_terms: D065966:Absorption, Physicochemical; D052436:Adipose Tissue, White / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D050154:Adiposity; D000818:Animals; D019440:Anti-Obesity Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D001938:Brazil; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements / Q000032:analysis; D005511:Food Handling; D006949:Hyperlipidemias / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D009765:Obesity / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D011897:Random Allocation; D015430:Weight Gain
chemical_list: D019440:Anti-Obesity Agents; D015415:Biomarkers; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Epigalocatechin-3-gallate; adipose tissue; lipid metabolism; lipolysis; obesity