title: Natriuretic peptides stimulate oocyte meiotic resumption in bovine.
abstract: The aim of the present study was to evaluate the expression of mRNA encoding natriuretic peptides (NPs) and their receptors in the cumulus-oocyte complex in cattle, a monovular mammalian species, and also to investigate the role of NPs in oocyte meiotic resumption in vitro. mRNA was observed for the NP precursor type-A (NPPA), type-C (NPPC), NP receptor-1 (NPR-1), receptor-2 (NPR-2) and receptor-3 (NPR-3) in bovine cumulus cells, and NPR-2 mRNA was observed in oocytes. These results are different from those obtained in mouse and pig models. The effects of NPPA, NP precursor type-B (NPPB) and NPPC on the resumption of arrested meiosis maintained by forskolin were studied at three different doses (10, 100 and 1000nM) with a 12h culture system. The germinal vesicle breakdown rates were greater (P≤0.05) in oocytes that were cultured in the presence of one or a combination of NPs (from 44% to 73%) than the negative control (from 24% to 27%). Additionally, it was demonstrated that the concentration of cyclic guanosine 3',5'-monophosphate (cGMP) is increased by NPPA and NPPC in oocytes and cumulus cells after 3h of in vitro maturation. However, in both groups, the concentration of cyclic adenosine 3',5'-monophosphate (cAMP) in the oocyte did not increase between 3 and 6h of culture, even when forskolin was used. In summary, we observed the presence of mRNA for NPs and their receptors in the bovine cumulus-oocyte complex and demonstrated that, in vitro, NPPA, NPPB and NPPC stimulate oocyte meiotic resumption in a monovular species.
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: vematheus.pdc@gmail.com.
authors.forename: Matheus P
authors.initials: MP
authors.lastname: De Cesaro
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Mariana P
authors.initials: MP
authors.lastname: Macedo
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Joabel T
authors.initials: JT
authors.lastname: Santos
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Paulo R A
authors.initials: PR
authors.lastname: Rosa
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Ludke
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Vitor B
authors.initials: VB
authors.lastname: Rissi
authors.affiliation: Laboratory of Animal Reproduction-ReproPEL, Federal University of Pelotas, Capão do Leão, RS, Brazil.
authors.forename: Bernardo G
authors.initials: BG
authors.lastname: Gasperin
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Paulo B D
authors.initials: PB
authors.lastname: Gonçalves
mesh_terms: D000818:Animals; D002417:Cattle; D054885:Cumulus Cells / Q000502:physiology*; D006152:Cyclic GMP / Q000502:physiology; D005260:Female; D008540:Meiosis / Q000502:physiology*; D020098:Natriuretic Peptide, C-Type / Q000502:physiology; D045265:Natriuretic Peptides / Q000502:physiology*; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D017461:Receptors, Atrial Natriuretic Factor / Q000502:physiology; D012697:Serine Endopeptidases / Q000502:physiology
chemical_list: D045265:Natriuretic Peptides; D020098:Natriuretic Peptide, C-Type; C119812:CORIN protein, human; D012697:Serine Endopeptidases; D017461:Receptors, Atrial Natriuretic Factor; C091715:atrial natriuretic factor receptor A; D006152:Cyclic GMP
keywords: Cattle; Cumulus-oocyte complex; Forskolin; Germinal vesicle breakdown