title: Microalgal growth with intracellular phosphorus for achieving high biomass growth rate and high lipid/triacylglycerol content simultaneously.
abstract: Nutrient deprivation is a commonly-used trigger for microalgal lipid accumulation, but its adverse impact on microalgal growth seems to be inevitable. In this study, Scenedesmus sp. LX1 was found to show similar physiological and biochemical variation under oligotrophic and eutrophic conditions during growth with intracellular phosphorus. Under both conditions microalgal chlorophyll content and photosynthesis activity was stable during this growth process, leading to significant increase of single cell weight and size. Therefore, while algal density growth rate dropped significantly to below 1.0 × 10(5)cells mL(-1) d(-1) under oligotrophic condition, the biomass dry weight growth rate still maintained about 40 mg L(-1) d(-1). Meanwhile, the lipid content in biomass and triacylglycerols (TAGs) content in lipids increased significantly to about 35% and 65%, respectively. Thus, high biomass growth rate and high lipid/TAG content were achieved simultaneously at the late growth phase with intracellular phosphorus. Besides, microalgal biomass produced was rich in carbohydrate with low protein content.
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory and State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China.
authors.forename: Yin-Hu
authors.initials: YH
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Research Center of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory and State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China. Electronic address: hyhu@tsinghua.edu.cn.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Hu
mesh_terms: D018533:Biomass; D048429:Cell Size; D002734:Chlorophyll / Q000378:metabolism; D050356:Lipid Metabolism; D058086:Microalgae / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010788:Photosynthesis; D044444:Scenedesmus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D014280:Triglycerides; D002734:Chlorophyll; D010758:Phosphorus
keywords: Biofuels; Biomass; Intracellular phosphorus; Lipid; Microalgae