title: Microbial surface displayed enzymes based biofuel cell utilizing degradation products of lignocellulosic biomass for direct electrical energy.
abstract: In this work, a bacterial surface displaying enzyme based two-compartment biofuel cell for the direct electrical energy conversion from degradation products of lignocellulosic biomass is reported. Considering that the main degradation products of the lignocellulose are glucose and xylose, xylose dehydrogenase (XDH) displayed bacteria (XDH-bacteria) and glucose dehydrogenase (GDH) displayed bacteria (GDH-bacteria) were used as anode catalysts in anode chamber with methylene blue as electron transfer mediator. While the cathode chamber was constructed with laccase/multi-walled-carbon nanotube/glassy-carbon-electrode. XDH-bacteria exhibited 1.75 times higher catalytic efficiency than GDH-bacteria. This assembled enzymatic fuel cell exhibited a high open-circuit potential of 0.80 V, acceptable stability and energy conversion efficiency. Moreover, the maximum power density of the cell could reach 53 μW cm(-2) when fueled with degradation products of corn stalk. Thus, this finding holds great potential to directly convert degradation products of biomass into electrical energy.
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Shuqin
authors.initials: S
authors.lastname: Fan
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors.forename: Chuantao
authors.initials: C
authors.lastname: Hou
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Feng
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China. Electronic address: liuah@qibebt.ac.cn.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
mesh_terms: D018407:Bacterial Physiological Phenomena; D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004735:Energy Transfer; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008031:Lignin / Q000378:metabolism*; D012037:Refuse Disposal / Q000295:instrumentation*
chemical_list: C036909:lignocellulose; D008031:Lignin; D002237:Carbohydrate Dehydrogenases
keywords: Degradation products of lignocellulosic biomass; Direct electrical energy conversion; Enzymatic biofuel cell; Glucose dehydrogenase displayed bacteria; Xylose dehydrogenase displayed bacteria