title: Quantification of osseointegration of plasma-polymer coated titanium alloyed implants by means of microcomputed tomography versus histomorphometry.
abstract: A common method to derive both qualitative and quantitative data to evaluate osseointegration of implants is histomorphometry. The present study describes a new image reconstruction algorithm comparing the results of bone-to-implant contact (BIC) evaluated by means of µCT with histomorphometry data. Custom-made conical titanium alloyed (Ti6Al4V) implants were inserted in the distal tibial bone of female Sprague-Dawley rats. Different surface configurations were examined: Ti6Al4V implants with plasma-polymerized allylamine (PPAAm) coating and plasma-polymerized ethylenediamine (PPEDA) coating as well as implants without surface coating. After six weeks postoperatively, tibiae were explanted and BIC was determined by µCT (3D) and afterwards by histomorphometry (2D). In comparison to uncoated Ti6Al4V implants demonstrating low BIC of 32.4% (histomorphometry) and 51.3% (µCT), PPAAm and PPEDA coated implants showed a nonsignificant increase in BIC (histomorphometry: 45.7% and 53.5% and µCT: 51.8% and 62.0%, resp.). Mean BIC calculated by µCT was higher for all surface configurations compared to BIC detected by histomorphometry. Overall, a high correlation coefficient of 0.70 (p < 0.002) was found between 3D and 2D quantification of BIC. The μCT analysis seems to be suitable as a nondestructive and accurate 3D imaging method for the evaluation of the bone-implant interface.
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Gabler
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Carmen
authors.initials: C
authors.lastname: Zietz
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Richard
authors.initials: R
authors.lastname: Bieck
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Göhler
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Lindner
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Haenle
authors.affiliation: Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany.
authors.forename: Birgit
authors.identifier: 0000-0002-5941-0376
authors.initials: B
authors.lastname: Finke
authors.affiliation: Institute of Physics, University of Greifswald, Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Meichsner
authors.affiliation: Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany ; Institute of Physics, University of Greifswald, Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Testrich
authors.affiliation: Department of Informatics and Electrical Engineering, Institute of Device Systems and Circuit Technology, University of Rostock, Albert-Einstein-Straße 2, 18059 Rostock, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Nowottnick
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Center Rostock, Schillingallee 35, 18057 Rostock, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Frerich
authors.affiliation: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Rainer
authors.identifier: 0000-0002-9157-1858
authors.initials: R
authors.lastname: Bader
mesh_terms: D000465:Algorithms; D000497:Alloys; D000818:Animals; D020099:Coated Materials, Biocompatible; D005029:Ethylenediamines; D005260:Female; D021621:Imaging, Three-Dimensional; D008422:Materials Testing; D016348:Osseointegration; D011073:Polyamines; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D014025:Titanium; D055114:X-Ray Microtomography
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D005029:Ethylenediamines; D011073:Polyamines; C031462:titanium alloy (TiAl6V4); C063994:polyallylamine; D014025:Titanium