title: Response of yeast cells to high glucose involves molecular and physiological differences when compared to other osmostress conditions.
abstract: Yeast cells can be affected by several causes of osmotic stress, such as high salt, sorbitol or glucose concentrations. The last condition is particularly interesting during natural processes where this microorganism participates. Response to osmostress requires the HOG (High Osmolarity Glycerol) pathway and several transcription factors, including Hot1, which plays a key role in high glucose concentrations. In this work, we describe how the yeast response to osmotic stress shows differences in accordance with the stress agent responsible for it. Compared with other conditions, under high glucose stress, delocalization of MAPK (Mitogen-Activated Protein Kinase) Hog1 is slower, induction of HOT1 expression is higher and Msn2/4 transcription factors are involved to a lesser extent. The transcriptomic analyses carried out with samples incubated for 30 min in the presence of high glucose or sorbitol reveal the presence of two functional categories with a differential expression between these conditions: glycogen biosynthesis and mobilization, and membrane-anchored proteins. We present data to demonstrate that the cells treated with 20% (w/v) (1.11 M) glucose contain higher chitin levels and are more sensitive to calcofluor white and ethanol.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr Moliner 50, E-46100 Burjassot (Valencia, Spain).
authors.forename: Mercè
authors.initials: M
authors.lastname: Gomar-Alba
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr Moliner 50, E-46100 Burjassot (Valencia, Spain).
authors.forename: Ma Ángeles
authors.initials: MÁ
authors.lastname: Morcillo-Parra
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr Moliner 50, E-46100 Burjassot (Valencia, Spain) m.del.olmo@uv.es.
authors.forename: Marcel Lí Del
authors.initials: ML
authors.lastname: Olmo
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D001557:Benzenesulfonates / Q000494:pharmacology; D002686:Chitin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009994:Osmolar Concentration; D009997:Osmotic Pressure / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D001557:Benzenesulfonates; D004268:DNA-Binding Proteins; C120535:HOT1 protein, S cerevisiae; C081935:MSN2 protein, S cerevisiae; D012097:Repressor Proteins; C083962:SKN7 protein, S cerevisiae; C077832:SKO1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D002686:Chitin; D000431:Ethanol; D013012:Sorbitol; C007061:C.I. Fluorescent Brightening Agent 28; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases; D005947:Glucose
keywords: Chitin; Hot1; Saccharomyces cerevisiae; gene expression; glycogen; osmotic stress