title: The effect and mechanism of miR196a in HepG2 cell.
abstract: OBJECTIVE
  To explore the role of miR-196a on the regulatory mechanism in hepatocelluar carcinoma.
  
  
  METHODS
  The antisense RNA of microRNA-196a was synthesized and cloned into the vector. HepG2 cells were infected by inhibiting miR196a vector. The HepG2 cells were divided into miR196a lower expression group, NC group and N group in vitro. The expression of the targets of miR-196a was detected by qPCR. Cell growth was analyzed by cck8 assay. The invasion was detected by transwell method. Apoptosis was detected by annexinV/PI. The P53, caspase-3, HOXB9, HOXB8 mRNA and their protein was detected by qPCR and Western-blot.
  
  
  RESULTS
  (1) The expression level of miR-196a was less than normal (41%). (2) The proliferation of HepG2 was also markedly suppressed in inhibiting miR196a at the 24 h point than normal about 72.29±2.51% (P<0.01). (3) The number of cells that migrated through the chamber of miR196a inhibiting group is less than normal and NC (P<0.01). (4) The cell apoptosis in miR196a inhibiting group is more than NC and normal group (P<0.05). HOXB8 mRNA and protein expression, in HepG2 cell line miR196a inhibiting group is significantly less than normal, NC (P<0.05). Caspase-3 mRNA and protein expression is maximum in three groups (P<0.05). In three groups there was no significant difference in the expression of P53 mRNA and protein and HOXB9mRNA.
  
  
  CONCLUSIONS
  Our results demonstrate that miR-196a can effect the proliferation, the apoptosis and migration of HepG2 cell lines by gene HOXB8, caspase-3 regulation. However, there is no correlation between miRNA196a and P53 and HOXB9.
authors.affiliation: Shanxi Provincial People's Hospital, 030012 Shanxi, Taiyuan, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Shanxi Provincial People's Hospital, 030012 Shanxi, Taiyuan, China. Electronic address: Junpingwang@gmail.com.
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shanxi Provincial People's Hospital, 030012 Shanxi, Taiyuan, China.
authors.forename: Longfeng
authors.initials: L
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C490669:HOXB8 protein, human; D018398:Homeodomain Proteins; C542543:MIRN196 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3
keywords: Caspase-3; HCC; HOXB8; HOXB9; P53; miR196