title: Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules.
abstract: Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnII) and three nodulation genes (nodA, nodC and nodD), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus Rhizobium. In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA-DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA-DNA hybridization showed 50-62% relatedness to their closest relatives (the type strains of Rhizobium etli and Rhizobium phaseoli) and 50-60% relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87-92% with their close relatives and 88-89% with each other. On the basis of these results, three novel species, Rhizobium lentis sp. nov. (type strain BLR27(T) = LMG 28441(T) = DSM 29286(T)), Rhizobium bangladeshense sp. nov. (type strain BLR175(T) = LMG 28442(T) = DSM 29287(T)) and Rhizobium binae sp. nov. (type strain BLR195(T) = LMG 28443(T) = DSM 29288(T)), are proposed. These species share common nodulation genes (nodA, nodC and nodD) that are similar to those of the symbiovar viciae.
authors.affiliation: Molecular Biology Laboratory, Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA), Bangladesh.|Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Germany.
authors.forename: M Harun-Or
authors.initials: MH
authors.lastname: Rashid
authors.affiliation: Department of Biology, University of York, UK.
authors.forename: J Peter W
authors.initials: JPW
authors.lastname: Young
authors.affiliation: Department of Biology, University of York, UK.
authors.forename: Isobel
authors.initials: I
authors.lastname: Everall
authors.affiliation: Laboratory of Microbiology, Ghent University, Belgium.
authors.forename: Pia
authors.initials: P
authors.lastname: Clercx
authors.affiliation: Laboratory of Microbiology, Ghent University, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Willems
authors.affiliation: Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Santhosh Braun
authors.affiliation: Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae; D005227:Fatty Acids / Q000737:chemistry; D027806:Lens Plant; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D009711:Nucleotides; D010802:Phylogeny; D018517:Plant Roots; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013559:Symbiosis / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009711:Nucleotides; D012336:RNA, Ribosomal, 16S