title: Native Chondrocyte Viability during Cartilage Lesion Progression: Normal to Surface Fibrillation.
abstract: OBJECTIVE
  Early surgical intervention for articular cartilage disease is desirable before full-thickness lesions develop. As early intervention treatments are designed, native chondrocyte viability at the treatment site before intervention becomes an important parameter to consider. The purpose of this study is to evaluate native chondrocyte viability in a series of specimens demonstrating the progression of articular cartilage lesions to determine if the chondrocyte viability profile changes during the evolution of articular cartilage disease to the level of surface fibrillation.
  
  
  METHODS
  Osteochondral specimens demonstrating various degrees of articular cartilage damage were obtained from patients undergoing knee total joint replacement. Three groups were created within a patient harvest based on visual and tactile cues commonly encountered during surgical intervention: group 1, visually and tactilely intact surfaces; group 2, visually intact, tactilely soft surfaces; and group 3, surface fibrillation. Confocal laser microscopy was performed following live/dead cell viability staining.
  
  
  RESULTS
  Groups 1 to 3 demonstrated viable chondrocytes in all specimens, even within the fibrillated portions of articular cartilage, with little to no evidence of dead chondrocytes. Chondrocyte viability profile in articular cartilage does not appear to change as disease lesion progresses from normal to surface fibrillation.
  
  
  CONCLUSIONS
  Fibrillated partial-thickness articular cartilage lesions are a good therapeutic target for early intervention. These lesions retain a high profile of viable chondrocytes and are readily diagnosed by visual and tactile cues during surgery. Early intervention should be based on matrix failure rather than on more aggressive procedures that further corrupt the matrix and contribute to chondrocyte necrosis of contiguous untargeted cartilage.
authors.affiliation: B-Division, Los Alamos National Laboratory, Los Alamos, NM, USA.
authors.forename: Kumkum
authors.initials: K
authors.lastname: Ganguly
authors.affiliation: NuOrtho Surgical, Inc., Fall River, MA, USA.
authors.forename: Ian D
authors.initials: ID
authors.lastname: McRury
authors.affiliation: Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, NM, USA.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Goodwin
authors.affiliation: NuOrtho Surgical, Inc., Fall River, MA, USA.
authors.forename: Roy E
authors.initials: RE
authors.lastname: Morgan
authors.affiliation: NuOrtho Surgical, Inc., Fall River, MA, USA ; Center for Orthopaedic and Sports Performance Research, Inc., Santa Fe, NM, USA.
authors.forename: Wayne K
authors.initials: WK
authors.lastname: Augé
keywords: cartilage; chondrocyte; fibrillated; partial thickness; viability