title: Expression of DROSHA in the Uterus of Mice in Early Pregnancy and Its Potential Significance During Embryo Implantation.
abstract: Previous studies have shown that microRNAs are involved in the process of implantation. They play an important role in cell growth and proliferation. DROSHA is the microRNA-processing enzyme and is required for the maturation of microRNAs. However, its expression and function during early pregnancy in mice still remain unclear. In the present study, we analyzed the expression pattern of DROSHA in the mouse uterus during early pregnancy, pseudopregnancy, artificially induced decidualization, and in the ovariectomized mouse uterus using real-time quantitative polymerase chain reaction, Western blotting analyses, and immunohistochemistry. We found that DROSHA was spatiotemporally expressed in decidualizing stromal cells during early pregnancy and in pseudopregnant mice in which decidualization was artificially induced. In the ovariectomized mouse uterus, the expression of DROSHA was upregulated after progesterone treatment. In a stromal cell culture model, the expression of DROSHA gradually increased with the progression of stromal decidualization. Taken together, our findings suggest that DROSHA is involved in stromal decidualization and may play an important role in embryo implantation in mice.
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Cuizhen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Long
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
authors.affiliation: Graduate School of Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Shangjing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Yingxiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China cqmu_liuxueqing@126.com.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D003656:Decidua / Q000378:metabolism; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D051379:Mice; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D011555:Pseudopregnancy / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011374:Progesterone; C513400:Drosha protein, mouse; D043244:Ribonuclease III
keywords: DROSHA; embryo implantation; microRNA