title: (-)-Epicatechin-3-gallate (a polyphenol from green tea) potentiates doxorubicin-induced apoptosis in H9C2 cardiomyocytes.
abstract: OBJECTIVE
  The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated.
  
  
  RESULTS
  Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomyocytes. The Bax/Bcl-2 ratio increased significantly after 1 h pretreatment with ECG. ECG also enhanced the phosphorylation of AMP-activated protein kinase (AMPK) which was induced by DOX in a dose-dependent manner.
  
  
  CONCLUSIONS
  Pretreatment with ECG sensitized H9C2 cells to DOX-mediated apoptosis through modulation of proteins involved in apoptosis and AMPK.
authors.affiliation: Guangzhou Research Institute of Snake Venom, Guangzhou Medical University, Guangzhou, 510182, Guangdong, People's Republic of China. yicaisysu@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, Guangdong, People's Republic of China.
authors.forename: Hui-Qi
authors.initials: HQ
authors.lastname: Hong
authors.affiliation: Guangzhou Research Institute of Snake Venom, Guangzhou Medical University, Guangzhou, 510182, Guangdong, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Guangzhou Key Laboratory of Materials for Energy Conversion and Storage, School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, Guangdong, People's Republic of China.
authors.forename: Yue-Peng
authors.initials: YP
authors.lastname: Cai
authors.affiliation: Guangzhou Research Institute of Snake Venom, Guangzhou Medical University, Guangzhou, 510182, Guangdong, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism; D002460:Cell Line; D004317:Doxorubicin / Q000378:metabolism*; D004347:Drug Interactions; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology*; D051381:Rats
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D002392:Catechin; C062669:epicatechin gallate
keywords: AMP-activated protein kinase; Apoptosis; Cardiomyocytes; Doxorubicin; Epicatechin-3-gallate; Green tea polyphenol; H9C2 cardiomyocytes