title: Comparative evaluation and correlation of salivary total antioxidant capacity and salivary pH in caries-free and severe early childhood caries children.
abstract: Dental caries is a major problem in preschool children. The contribution of saliva in providing defense during caries process is of primary importance. pH buffer capacity through bicarbonate, phosphate and protein buffer systems have universal acceptance as a caries defense mechanism. Antioxidant capacity of saliva can constitute a first line of defense against chronic degenerative diseases including dental caries. Till date, no study is presented with salivary antioxidant capacity of younger children affected with severe early childhood caries with its salivary pH correlation. Hence, this study was carried out to compare, evaluate and correlate the salivary total antioxidant capacity (TAC) and salivary pH of children with caries-free and severe early childhood caries.
  
  
  
  Fifty children from ages 3 to 5 years divided into two study groups had undergone screening. Group I (n = 25) with severe early childhood caries (S-ECC) and group II (n = 25) who were caries free. Unstimulated whole saliva of subjects were in the collection during the study by draining method. Salivary pH determination of saliva samples was done using pH indicator paper strips. The TAC was done using an antioxidant assay with the help of a spectrophotometer at wavelength 532 nm. The means of salivary pH and TAC were subjected to analysis using unpaired student 't' test and correlation was determined using Pearsons correlation coefficient analysis.
  
  
  
  Mean salivary pH was higher in group II (7.46 ± 0.37). Mean TAC was greater in group I (1.82 ± 0.19). A statistically significant negative correlation as seen between TAC and salivary pH in S-ECC patients.
  
  
  
  The study concludes that salivary TAC increases in patients with S-ECC are by that showing a high indirect relationship with salivary pH.
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Dr DY Patil Dental College and Hospital, Dr DY Patil Vidyapeeth, Pune, Maharashtra, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Muchandi
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Dr DY Patil Dental College and Hospital, Dr DY Patil Vidyapeeth, Pune, Maharashtra, India.
authors.forename: Hrishikesh
authors.initials: H
authors.lastname: Walimbe
authors.affiliation: Assistant Professor, Department of Preventive Dental Sciences, Division of Pedodontics, King Khalid University, College of Dentistry, Abha, Saudi Arabia, Phone: + 966-(0)50-4217533, e-mail: info@drnadeembijle.com.
authors.forename: Mohammed Nadeem Ahmed
authors.initials: MN
authors.lastname: Bijle
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Dr DY Patil Dental College and Hospital, Dr DY Patil Vidyapeeth, Pune, Maharashtra, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Nankar
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Dr DY Patil Dental College and Hospital, Dr DY Patil Vidyapeeth, Pune, Maharashtra, India.
authors.forename: Srishti
authors.initials: S
authors.lastname: Chaturvedi
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Dr DY Patil Dental College and Hospital, Dr DY Patil Vidyapeeth, Pune, Maharashtra, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Karekar
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002021:Buffers; D002675:Child, Preschool; D003731:Dental Caries / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012463:Saliva / Q000737:chemistry* / Q000502:physiology; D013053:Spectrophotometry / Q000295:instrumentation; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D000975:Antioxidants; D002021:Buffers; D017392:Thiobarbituric Acid Reactive Substances
keywords: Dental caries; Oxidative stress; Saliva