title: Blood Collection from Mice and Hematological Analyses on Mouse Blood.
abstract: Basic phenotyping of inbred mouse strains and genetically modified mouse models usually includes the determination of blood-based parameters as a diagnostic screen for genotype effects on metabolism and organ function. A broad range of analytes, including hematological parameters, can be reliably determined in mouse blood, if appropriate samples are available. Here we describe recommended techniques for blood collection from mice and the considerations that have to be taken into account to get adequate samples for hematological investigations. Furthermore, we describe established methods used in the German Mouse Clinic (GMC) to determine hematological parameters in the mouse. Curr. Protoc. Mouse Biol. 3:101-119 © 2013 by John Wiley & Sons, Inc.
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.|Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Rathkolb
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Fuchs
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gailus-Durner
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Aigner
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
authors.affiliation: Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Research Center for Environmental Health, GmbH, Neuherberg, Germany.|Institute of Experimental Genetics, Life and Food Science Center Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.|German Research Center for Diabetes Research, Neuherberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hrabě de Angelis
keywords: blood sample collection; hematology; mouse