title: Effects of glycerol on enzymatic hydrolysis and ethanol production using sugarcane bagasse pretreated by acidified glycerol solution.
abstract: In this study, for the first time the effects of glycerol on enzymatic hydrolysis and ethanol fermentation were investigated. Enzymatic hydrolysis was inhibited slightly with 2.0 wt% glycerol, leading to reduction in glucan digestibility from 84.9% without glycerol to 82.9% (72 h). With 5.0 wt% and 10.0 wt% glycerol, glucan digestibility was reduced by 4.5% and 11.0%, respectively. However, glycerol did not irreversibly inhibit cellulase enzymes. Ethanol fermentation was not affected by glycerol up to 5.0 wt%, but was inhibited slightly at 10.0 wt% glycerol, resulting in reduction in ethanol yield from 86.0% in the absence of glycerol to 83.7% (20 h). Based on the results of laboratory and pilot-scale experiments, it was estimated that 0.142 kg ethanol can be produced from 1.0 kg dry bagasse (a glucan content of 38.0%) after pretreatment with acidified glycerol solution.
authors.affiliation: Syngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia. Electronic address: jan.zhang@qut.edu.au.
authors.forename: Zhanying
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia.
authors.forename: Heng H
authors.initials: HH
authors.lastname: Wong
authors.affiliation: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Albertson
authors.affiliation: Syngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Harrison
authors.affiliation: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia.
authors.forename: William O S
authors.initials: WO
authors.lastname: Doherty
authors.affiliation: Syngenta Centre for Sugarcane Biofuels Development, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2432, 2 George St, Brisbane, QLD 4001, Australia.
authors.forename: Ian M
authors.initials: IM
authors.lastname: O'Hara
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry*; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry; D010865:Pilot Projects; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
chemical_list: D005936:Glucans; D052578:Ionic Liquids; D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D005990:Glycerol
keywords: Enzymatic hydrolysis; Fermentation; Glycerol; Pretreatment; Sugarcane bagasse