title: Design, characterization and skin permeating potential of Fluocinolone acetonide loaded nanostructured lipid carriers for topical treatment of psoriasis.
abstract: The aim of the current study was to develop and optimize Fluocinolone acetonide (FA) loaded nanostructured lipid carriers (NLC) and to evaluate its potential as topical delivery system for management of psoriasis. FA loaded NLCs were successfully developed by modified microemulsion method and optimized using 3-level Box-Behnken design. NLCs were evaluated for particle size, polydispersity index, zeta potential, drug entrapment efficiency and drug loading. Further X-ray diffraction (XRD) and Differential scanning calorimetry (DSC), in vitro release, in vitro skin distribution and stability study were also performed. Transmission electron microscopy confirmed spherical shape of prepared NLCs. Complete encapsulation of drug in the nanoparticles was confirmed by XRD and DSC. Release study showed prolonged drug release from the NLCs following Higuchi release kinetics and Zero order release kinetics, whereas pure FA suspension exhibited faster drug release following Zero order release kinetics with R(2) value of 0.995. Stability study confirmed that NLCs were stable for 3months at 4°C. Furthermore, in vitro skin distribution studies showed presence of significant amount of FA in the epidermal and dermal layer of skin when treated with FA loaded NLCs suspension while plain FA suspension showed significantly lesser amount of FA in the epidermis and dermis. Moreover, selective retention of FA in the epidermis might eliminate adverse side effects associated with systemic exposure. Thus FA loaded NLCs could be a potential system for psoriasis treatment but to create clinical value of the present system further studies are needed in clinically relevant models.
authors.affiliation: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010, India.
authors.forename: Madhulika
authors.initials: M
authors.lastname: Pradhan
authors.affiliation: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010, India; NCNPR, School of Pharmacy, University of Mississippi, Oxford, MS, USA.
authors.forename: Deependra
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, University of Mississippi, Oxford, MS, USA.
authors.forename: S Narasimha
authors.initials: SN
authors.lastname: Murthy
authors.affiliation: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010, India; Department of Pharmaceutics, School of Pharmacy, University of Mississippi, Oxford, MS, USA. Electronic address: manjursu@rediffmail.com.
authors.forename: Manju Rawat
authors.initials: MR
authors.lastname: Singh
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D015195:Drug Design; D005446:Fluocinolone Acetonide / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D006041:Goats; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011565:Psoriasis / Q000188:drug therapy*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012984:Software
chemical_list: D004337:Drug Carriers; D008055:Lipids; D005446:Fluocinolone Acetonide
keywords: Box–Behnken design; Fluocinolone acetonide; Nano structured lipid carriers; Psoriasis; Topical delivery