title: Human cytomegalovirus microRNA miR-US25-1-5p inhibits viral replication by targeting multiple cellular genes during infection.
abstract: MicroRNAs (miRNAs) play important roles in regulating various cellular processes in plants, animals, and viruses. This mechanism is also utilized by human cytomegalovirus (HCMV) in the process of infection and pathogenesis. The HCMV-encoded miRNA, hcmv-miR-US25-1-5p, was highly expressed during lytic and latent infections, and was found to inhibit viral replication. Identification of functional target genes of this microRNA is important in that it will enable a better understanding of the function of hcmv-miR-US25-1-5p during HCMV infection. In the present study, 35 putative cellular transcript targets of hcmv-miR-US25-1-5p were identified. Down-regulation of the targets YWHAE, UBB, NPM1, and HSP90AA1 by hcmv-miR-US25-1-5p was validated by luciferase reporter assay and Western blot analysis. In addition, we showed that hcmv-miR-US25-1-5p could inhibit viral replication by interacting with these targets, the existence of which may impact virus replication directly or indirectly.
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China; Clinical Genetics, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Clinical Genetics, The Affiliated Shengjing Hospital, China Medical University, China. Electronic address: her@sj-hospital.org.
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Yaozhong
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, China. Electronic address: ruanq@sj-hospital.org.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D000090243:Nucleophosmin; D034622:RNA Interference; D012367:RNA, Viral / Q000502:physiology; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs; C000716967:NPM1 protein, human; D012367:RNA, Viral; D000090243:Nucleophosmin
keywords: DNA replication; Down-regulation; Human cytomegalovirus; Target genes; hcmv-miR-US25-1-5p