title: High-Resolution Respirometry for Mitochondrial Characterization of Ex Vivo Mouse Tissues.
abstract: This article describes methodologies to examine mitochondrial respiration in fresh preparations of mouse tissues, including skeletal muscle, heart, liver, white and brown adipose tissue, and brain. Reference values and tips to maximize experimental efficiencies are also provided. Finally, correction methods and complementary techniques to properly interpret the results are presented and contrasted.
authors.affiliation: Nestlé Institute of Health Sciences S.A, Lausanne, Switzerland.
authors.forename: Carles
authors.initials: C
authors.lastname: Cantó
authors.affiliation: Diabetes and Obesity Research Laboratory, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS) and Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Barcelona, Spain.
authors.forename: Pablo M
authors.initials: PM
authors.lastname: Garcia-Roves
mesh_terms: D000825:Animal Structures / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D019069:Cell Respiration; D051379:Mice; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D010100:Oxygen
keywords: bioenergetics; mitochondrial physiology; respiratory complexes