title: Chemical fingerprinting of Gardenia jasminoides Ellis by HPLC-DAD-ESI-MS combined with chemometrics methods.
abstract: A fingerprint analysis method has been developed for characterization and discrimination of Gardenia jasminoides Ellis from different areas. The chemometrics methods including similarity evaluation, principal components analysis (PCA) and hierarchical clustering analysis (HCA) were introduced to identify more useful chemical markers for improving the quality control standard of dried ripe fruits of G. jasminoides Ellis. Then the selected chemical markers were analyzed by high performance liquid chromatography-diode array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) qualitatively and quantitatively. 23 characteristic peaks were assigned while 19 peaks of them were identified by comparing retention times, UV and MS spectra with authentic compounds or literature data. Moreover, 14 of them were determined quantitatively which could effectively evaluate the quality of G. jasminoides Ellis. This study was expected to provide comprehensive information for the quality evaluation of G. jasminoides Ellis, which would be a valuable reference for further study and development of this herb and related medicinal products.
authors.affiliation: School of Pharmacy, The Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Pharmacy, The Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wen
authors.affiliation: School of Pharmacy, The Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: tingting_zoo@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, The Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: guorfan@163.com.
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D032062:Gardenia / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D025341:Principal Component Analysis; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
keywords: Gardenia jasminoides Ellis; HPLC–DAD–ESI-MS; Hierarchical clustering analysis (HCA); Principal components analysis (PCA); Quality evaluation; Similarity evaluation