title: A critical role of mir-199a in the cell biological behaviors of colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is one of the most common cancer and the leading causes of cancer mortality worldwide. The critical role of hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) are important in the cancer development.
  
  
  METHODS
  The purpose of this study was to investigate the association of miR-199a expression in CRC and non-tumor tissues as well as assessed the effect of miR-199a on biological behaviors including cell proliferation, apoptosis, migration and invasion of CRC cells. The expression of miR-199a was distinctly decreased in colorectal cancer tissues compared with non-neoplastic colorectal tissues.
  
  
  RESULTS
  In this study, we found that miR-199a down-regulation was associated with the CRC and metastasis incidence. Advanced study showed that miR-199a up-regulation would lead to decreased CRC proliferation, migration and invasion. However, no significant association of miR-199a treatment and apoptosis rate and cell-cycle were detected in this study. The detection for the mechanisms of miR-199a on the development of CRC showed that the anticarcinogenic effect of miR-199a might be produced through HIF-1α/VEGF pathway.
  
  
  CONCLUSIONS
  It was found that miR-199a would reduce the proliferation, migration and invasion. However, overexpression of miR-199a on the apoptosis rate and cell cycles showed no significant results. The potential functionary mechanism of miR-199a might through HIF-1α/VEGF pathway.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/9806714131513041 .
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. yehua_gz@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. panglipinggz@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Pang
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. wu_qiong_09@163.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. drzheng_gu@163.com.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. ppdqj1@163.com.
authors.forename: Cancan
authors.initials: C
authors.lastname: Guo
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. pphuabai@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang, Guangdong, 524023, China. zheng_xuebao@163.com.
authors.forename: Xuebao
authors.initials: X
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D035683:MicroRNAs; C531408:mirn199 microRNA, human