title: MicroRNA-125-5p targeted CXCL13: a potential biomarker associated with immune thrombocytopenia.
abstract: BACKGROUND
  Immune thrombocytopenia (ITP) is an acquired and autoimmune disease of adults and children characterized by decreased platelet production. CXC chemokine ligand-13 (CXCL13) participates in multiple immunological responses. However, it is still unknown the relationship between CXCL13 and ITP.
  
  
  METHODS
  Plasma CXCL13 was detected in ITP (n = 30) children. CD4+ T cells was isolated from peripheral blood mononuclear cells (PBMCs) from healthy volunteers. Treated CD4+ T cells with dexamethasone and/or miR-125-5p mimic/inhibitor, to observe the regulation of CXCL13.
  
  
  RESULTS
  Compared with controls, ITP children had elevated plasma CXCL13, the concentration of which was reduced after treatment. In vitro, dexamethasone decreased CXCL13 level in in dose- dependent and in time-dependent manner. MiR-125-5p mimic decreased CXCL13 level and miR-125-5p inhibitor increased CXCL13 level in CD4+ T cells. CXCL13 was implied to be target gene of miR-125-5p. MiR-125-5p inhibitor also canceled dexamethasone induced decrease of CXCL13.
  
  
  CONCLUSIONS
  CXCL13 is the target gene of miR-125-5p, which is possibly involved in the pathological process of ITP.
authors.affiliation: Department of Hematology, Soochow University Affiliated Children's Hospital Suzhou 215003, Jiangsu, China.
authors.forename: Jian-Qin
authors.initials: JQ
authors.lastname: Li
authors.affiliation: Department of Hematology, Soochow University Affiliated Children's Hospital Suzhou 215003, Jiangsu, China.
authors.forename: Shao-Yan
authors.initials: SY
authors.lastname: Hu
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University Suzhou 215016, Jiangsu, China.
authors.forename: Zhao-Yue
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Department of Hematology, Soochow University Affiliated Children's Hospital Suzhou 215003, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University Suzhou 215016, Jiangsu, China.
authors.forename: Su
authors.initials: S
authors.lastname: Jian
authors.affiliation: Department of Hematology, Soochow University Affiliated Children's Hospital Suzhou 215003, Jiangsu, China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Dong
authors.affiliation: Department of Hematology, Soochow University Affiliated Children's Hospital Suzhou 215003, Jiangsu, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Wu
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University Suzhou 215016, Jiangsu, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Lan
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University Suzhou 215016, Jiangsu, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Cao
keywords: Immune thrombocytopenia; chemokine; lymphocytes; miR-125-5p