title: Laparoscopic endometrioma resection increases peri-implantation endometrial HOXA-10 and HOXA-11 mRNA expression.
abstract: OBJECTIVE
  To determine whether laparoscopic endometrioma resection alters peri-implantation endometrial HOXA-10, HOXA-11, LIF, ITGB3 and ITGAV mRNA expression.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  Medical school.
  
  
  METHODS
  Twenty infertile patients with uni- or bilateral endometrioma, five infertile patients having nonendometriotic benign ovarian cyst, and five fertile control subjects.
  
  
  METHODS
  Mid-luteal-phase endometrial sampling was performed at the time of surgery. Second endometrial biopsies were obtained 3 months after laparoscopic endometrioma resection during the mid-luteal phase of the cycle.
  
  
  METHODS
  Endometrial HOXA-10, HOXA-11, LIF, ITGAV, and ITGB3 mRNA expressions were evaluated with the use of reverse-transcription polymerase chain reaction.
  
  
  RESULTS
  Significantly decreased endometrial ITGAV mRNA expression was noted in biopsies obtained from endometrioma and nonendometriotic cyst groups before surgery. Trends toward decreased endometrial HOXA-10, HOXA-11, LIF, and ITGB3 mRNA expressions were noted in the endometrioma and nonendometriotic cyst groups before surgery compared with the fertile subjects. However, the differences failed to show statistical significance. Compared with preoperative values, significantly increased HOXA-10 (12.1-fold change) and HOXA-11 (17.2-fold change) mRNA expressions were noted in endometrial biopsies obtained from subjects who were undergoing endometrioma surgery. Fold change in endometrial ITGAV mRNA after endometrioma surgery was found to be 30.1 and indicated a positive regulation. However, this fold increase was statistically insignificant. Expressions of these endometrial receptivity markers did not change significantly after surgical removal of nonendometriotic benign ovarian cysts.
  
  
  CONCLUSIONS
  Laparoscopic endometrioma resection increases peri-implantation endometrial HOXA-10 and HOXA-11 mRNA expression, suggesting an improvement in endometrial receptivity.
authors.affiliation: Private Clinic, Obstetrics and Gynecology, Usak, Turkey. Electronic address: celiksudenaz@gmail.com.
authors.forename: Onder
authors.initials: O
authors.lastname: Celik
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Acıbadem University, Istanbul, Turkey.
authors.forename: Cihat
authors.initials: C
authors.lastname: Unlu
authors.affiliation: Department of Medical Microbiology, Inonu University, School of Medicine, Malatya, Turkey.
authors.forename: Baris
authors.initials: B
authors.lastname: Otlu
authors.affiliation: Department of Biochemistry, Behçet Uz Children's Hospital, İzmir, Turkey.
authors.forename: Nilufer
authors.initials: N
authors.lastname: Celik
authors.affiliation: Department of Obstetrics and Gynecology, Medical Park Hospital, Kocaeli, Turkey.
authors.forename: Eray
authors.initials: E
authors.lastname: Caliskan
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000378:metabolism* / Q000601:surgery; D004717:Endometrium / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005786:Gene Expression Regulation; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D010535:Laparoscopy / Q000639:trends*; D019836:Preimplantation Diagnosis / Q000639:trends; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: C484298:HOXA11 protein, human; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; C092119:Hoxa10 protein, mouse
keywords: Endometrioma; eutopic endometrium; homeobox genes; laparoscopy