title: Continuous Flow Microfluidic Bioparticle Concentrator.
abstract: Innovative microfluidic technology has enabled massively parallelized and extremely efficient biological and clinical assays. Many biological applications developed and executed with traditional bulk processing techniques have been translated and streamlined through microfluidic processing with the notable exception of sample volume reduction or centrifugation, one of the most widely utilized processes in the biological sciences. We utilize the high-speed phenomenon known as inertial focusing combined with hydraulic resistance controlled multiplexed micro-siphoning allowing for the continuous concentration of suspended cells into pre-determined volumes up to more than 400 times smaller than the input with a yield routinely above 95% at a throughput of 240 ml/hour. Highlighted applications are presented for how the technology can be successfully used for live animal imaging studies, in a system to increase the efficient use of small clinical samples, and finally, as a means of macro-to-micro interfacing allowing large samples to be directly coupled to a variety of powerful microfluidic technologies.
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Martel
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Kyle C
authors.initials: KC
authors.lastname: Smith
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Mcolisi
authors.initials: M
authors.lastname: Dlamini
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Kendall
authors.initials: K
authors.lastname: Pletcher
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Yang
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Murat
authors.initials: M
authors.lastname: Karabacak
authors.affiliation: Massachusetts General Hospital Cancer Center, Boston, Massachusetts, 02114, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Haber
authors.affiliation: BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.
authors.forename: Ravi
authors.initials: R
authors.lastname: Kapur
authors.affiliation: 1] BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA [2] Shriners Hospital for Children, Boston, Massachusetts, 02114, USA.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
mesh_terms: D002498:Centrifugation; D005434:Flow Cytometry / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*