title: In vivo quantification of cochlin in glaucomatous DBA/2J mice using optical coherence tomography.
abstract: The expression of cochlin in the trabecular meshwork (TM) precedes the clinical glaucoma symptoms in DBA/2J mice. The ability to quantify cochlin in the local tissue (TM) offers potential diagnostic and prognostic values. We present two (spectroscopic and magnetomotive) optical coherence tomography (OCT) approaches for in vivo cochlin quantification in a periodic manner. The cochlin-antibody OCT signal remains stable for up to 24 hours as seen at 3.5 hours after injection allowing for repeated quantification in the living mouse eyes.
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136.
authors.forename: Ayman
authors.initials: A
authors.lastname: Aljohani
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136.
authors.forename: Teresia
authors.initials: T
authors.lastname: Carreon
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Gregori
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136.
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005123:Eye / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D005901:Glaucoma / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007429:Intraocular Pressure; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D018613:Microscopy, Confocal; D008875:Middle Aged; D011859:Radiography; D014019:Tissue Donors; D041623:Tomography, Optical Coherence; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism
chemical_list: C110668:COCH protein, human; C491729:Coch protein, mouse; D016326:Extracellular Matrix Proteins