title: Timing readout in paper device for quantitative point-of-use hemin/G-quadruplex DNAzyme-based bioassays.
abstract: This work describes a quantitative point-of-use hemin/G-quadruplex DNAzyme-based assay that integrates a simple timing detection motif with low-cost, portable microfluidic paper-based analytical devices (μPADs). The timing readout is based on the selective DNAzyme-mediated wettability change of paper from hydrophilicity to hydrophobicity. Its utility is well demonstrated with sensitive, specific detection of K(+) ion as a model analyte in artificial samples as well as real human serum samples. This new method only requires a ubiquitous cheap timer (or a cell phone with a timing function) to provide quantitative results. It could offer new opportunities for the development of more peroxidase-mimicking DNAzyme-based bioassays that are simple, affordable, portable, and operable by minimally-trained users for broad point-of-use applications especially in resource-poor settings.
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China. Electronic address: zy@glut.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Fan
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China. Electronic address: Niejinfang@glut.edu.cn.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Nie
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Shangwang
authors.initials: S
authors.lastname: Le
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Gao
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
mesh_terms: D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes; D021881:DNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054856:G-Quadruplexes; D006427:Hemin; D006801:Humans; D057230:Limit of Detection; D010209:Paper; D019095:Point-of-Care Systems; D011188:Potassium / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D015342:DNA Probes; D021881:DNA, Catalytic; D006427:Hemin; D011188:Potassium
keywords: DNAzyme; Microfluidic paper-based analytical device; Point-of-use assay; Potassium ion; Timing detection