title: CYP1B1 deficiency ameliorates obesity and glucose intolerance induced by high fat diet in adult C57BL/6J mice.
abstract: Cytochrome P450 1B1 (CYP1B1) expression increases in multi-potential mesenchymal stromal cells C3H10T1/2 during adipogenesis, which parallel with PPARγ, a critical transcriptional factor in adipogenic process. To assess the role of CYP1B1 in fatty acid metabolism, adult C57BL/6J wild-type and CYP1B1 deficiency mice were fed with high fat diets (HFD) for 6 weeks. CYP1B1 deficiency attenuated HFD-induced obesity when compared with their wild type counterparts, and improve glucose tolerance. The reduction in body weight gain and white adipose tissue in CYP1B1 deficient mice exhibited coordinate decreases in fatty acid synthesis (PPARγ, CD36, FAS, SCD-1) and increases in fatty acid oxidation (UCP-2, CPT-1a) when compared with wild type ones. Lower hepatocyte TG contents were consistent with hepatic Oil-Red-O staining in the CYP1B1 deficiency mice. AMPK, a nutrient sensors for energy homeostasis, was activated in both fat pad and liver by CYP1B1 deficiency. However, in vitro system, knock down CYP1B1 in C3H10T1/2 cells does not abolish adipogenesis induced by adipogenic agents IDM (Insulin, Dexamethasone, Methylisobutylxanthine). Our in vivo and in vitro findings of CYP1B1 deficiency in fat metabolism suggest a complex regulation network between CYP1B1 and energy homeostasis.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Yumeng
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Yatao
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China ; Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Academy for Preventive Medicine China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Fan
keywords: CYP1B1; diet induced obesity (DIO); fat metabolism; glucose intolerance; high fat diet (HFD)