title: Coupling of Molecular Imprinted Polymer Nanoparticles by High Performance Liquid Chromatography as an Efficient Technique for Sensitive and Selective Trace Determination of 4-Chloro-2-Methylphenoxy Acetic Acid in Complex Matrices.
abstract: BACKGROUND
  4-chloro-2-methylphenoxy acetic acid (MCPA) is one of the most important pesticides which is extensively used to control weeds in arable farmland. Exposure to this compound occurs in general population and persons who occupationally handle it. The aim of this present work was the preparation of MCPA imprinting polymer and its application as a selective sample preparation technique for trace determination of MCPA in biological and environmental samples.
  
  
  METHODS
  In this study, MCPA imprinting polymer was obtained by precipitation polymerization using methacrylic acid (the functional monomer), ethylene glycol dimethacrylate (the cross-linker), 2, 2'-azobisisobutyronitrile (the initiator) and MCPA (the template molecule) in acetonitrile solution. The MIP-NPs were characterized by thermogravimetric analysis and scanning electron microscopy. The optimization process was carried out applying batch method. After optimization of the parameters, affecting the adsorption and desorption of analyte, urine and different water samples were used to determine MCPA.
  
  
  RESULTS
  Imprinted MCPA molecules were removed from the polymeric structure using acetic acid in methanol (20:80 v/v %) as the eluting solvent. Both sorption and desorption process occur within 10 min. The maximum sorbent capacity of the molecular imprinted polymer is 87.4 mg g-1. The relative standard deviation and limit of detection for water samples by introduced selective solid phase extraction were 4.8% and 0.9 μg L-1, and these data for urine samples were 4.5% and 1.60 μg L-1, respectively.
  
  
  CONCLUSIONS
  The developed method was successfully applied to determine MCPA in urine and different water samples.
authors.affiliation: 1. Dept. of Occupational Health Engineering, School of Public Health, Shahroud University of Medical Sciences , Shahroud, Iran.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Omidi
authors.affiliation: 2. Dept. of Chemistry, Faculty of Science, Shahid Beheshti University, G.C. , Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Behbahani
authors.affiliation: 2. Dept. of Chemistry, Faculty of Science, Shahid Beheshti University, G.C. , Tehran, Iran.
authors.forename: Saadi
authors.initials: S
authors.lastname: Samadi
authors.affiliation: 3. Dept. of Occupational Health, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Sedighi
authors.affiliation: 4. Dept. of Occupational Health Engineering, School of Public Health, Institute for Environmental Research, Tehran University of Medical Sciences , Tehran, Iran.
authors.forename: Seyed Jamaleddin
authors.initials: SJ
authors.lastname: Shahtaheri
keywords: 4-chloro-2-methylphenoxy acetic acid; Molecular imprinted polymer nanoparticles; Selective preconcentration; Urine and water samples.