title: A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization.
abstract: OBJECTIVE
  Therapeutic efficacy is routinely assessed by measurement of lesion size using flatmounted choroids and confocal microscopy in the laser-induced choroidal neovascularization (L-CNV) rodent model. We investigated whether optical coherence tomography (OCT) quantification, using an ellipsoid volume measurement, was comparable to standard ex vivo evaluation methods for this model and whether this approach could be used to monitor treatment-related lesion changes.
  
  
  METHODS
  Bruch's membrane was ruptured by argon laser in the dilated eyes of C57BL/6J mice, followed by intravitreal injections of anti-VEGF164 or vehicle, or no injection. In vivo OCT images were acquired using Micron III or InVivoVue systems at 7, 10, and/or 14 days post-laser and neovascular lesion volume was calculated as an ellipsoid. Subsequently, lesion volume was compared to that calculated from confocal Z-stack images of agglutinin-stained choroidal flatmounts.
  
  
  RESULTS
  Ellipsoid volume measurement of orthogonal 2-dimensional OCT images obtained from different imaging systems correlated with ex vivo lesion volumes for L-CNV (Spearman's ρ=0.82, 0.75, and 0.82 at days 7, 10, and 14, respectively). Ellipsoid volume calculation allowed temporal monitoring and evaluation of CNV lesions in response to antivascular endothelial growth factor treatment.
  
  
  CONCLUSIONS
  Ellipsoid volume measurements allow rapid, quantitative use of OCT for the assessment of CNV lesions in vivo. This novel method can be used with different OCT imaging systems with sensitivity to distinguish between treatment conditions. It may serve as a useful adjunct to the standard ex vivo confocal quantification, to assess therapeutic efficacy in preclinical models of CNV, and in models of other ocular diseases.
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.|3 Department of Pharmacology and Toxicology, Indiana University School of Medicine , Indianapolis, Indiana.|4 Department of Biochemistry, Faculty of Pharmacy, Cairo University , Cairo, Egypt .
authors.forename: Rania S
authors.initials: RS
authors.lastname: Sulaiman
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.
authors.forename: Judith
authors.initials: J
authors.lastname: Quigley
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Qi
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.|5 School of Biomedical Science, University of Ulster , Coleraine, Northern Ireland, United Kingdom .
authors.forename: Michael N
authors.initials: MN
authors.lastname: O'Hare
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.
authors.forename: Maria B
authors.initials: MB
authors.lastname: Grant
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Boulton
authors.affiliation: 1 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.|2 Department of Ophthalmology, Indiana University School of Medicine , Indianapolis, Indiana.|3 Department of Pharmacology and Toxicology, Indiana University School of Medicine , Indianapolis, Indiana.|6 Department of Biochemistry and Molecular Biology, Indiana University School of Medicine , Indianapolis, Indiana.|7 Indiana University Melvin and Bren Simon Cancer Center , Indianapolis, Indiana.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Corson
mesh_terms: D000818:Animals; D016570:Bruch Membrane / Q000601:surgery; D002829:Choroid / Q000378:metabolism; D020256:Choroidal Neovascularization / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D005451:Fluorescein Angiography / Q000379:methods; D058449:Intravitreal Injections; D017075:Laser Coagulation / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods; D015203:Reproducibility of Results; D041623:Tomography, Optical Coherence / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors
chemical_list: D042461:Vascular Endothelial Growth Factor A