title: Synthesis of carbon-14, carbon-13 and deuterium labeled forms of thioacetamide and thioacetamideS-oxide.
abstract: Thioacetamide (TA) is a model hepatotoxin that undergoes metabolic activation via two successiveS-oxidations. The ultimate toxic metabolite thioacetamideS,S-dioxide, or its tautomer acetimidoyl sulfinic acid CH3C(NH)SO2H, then acylates lysine side chains on cellular proteins leading to cellular dysfunction or death. To identify individual target proteins, quantitate the extent of their modification and elucidate the structural details of their modification we required both radio-labeled and stable-labeled forms of TA and its intermediate metabolite thioacetamideS-oxide (TASO). The latter is stable when purified but can be difficult to isolate. Considering currently available isotopic precursors we devised and report here methods for the synthesis and isolation of TA and TASO labeled with C-14, C-13 and/or deuterium. The methods are straightforward, utilize readily available precursors and are amenable to small scale.
authors.affiliation: Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045, USA.
authors.forename: Diganta
authors.initials: D
authors.lastname: Sarma
authors.affiliation: Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045, USA.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Hanzlik
keywords: acetate; deuterium exchange; pyrolysis; thioacetamide; thioacetamide S-oxide