title: Expression of vascular endothelial growth factor and basic fibroblast growth factor in extramammary Paget disease.
abstract: Extramammary Paget's disease (EMPD) is a special type of cancers. The etiology of the disease is still unclear. We aimed to study the expression differences of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in EMPD tissues and corresponding adjacent normal tissues. The mRNA expression was detected by RT-PCR and the protein expression was explored by immunohistochemistry. Higher immunostaining signal scores of bFGF and VEGF in EMPD tissues had been found (z=-3.827, P<0.001, z=-3.729, P<0.001, respectively). In addition, the mRNA expression of bFGF and VEGF was higher in EMPD tissues, which had been validated by RT-PCR (t=5.771, P<0.001, t=3.304, P=0.004, respectively). The VEGF and bFGF might be the key signaling proteins in angiogenesis of EMPD. How to block the VEGF and bFGF in EMPD and to destroy the blood supply of the tumor cells becomes the focus of our future research.
authors.affiliation: Department of Dermatology, Jiangsu Province Geriatric Hospital 65 Jiangsu Road, Nanjing 210024, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Second People's Hospital of Wuxi Affiliated to Nanjing Medical University 68 Zhongshan Road, Wuxi 214002, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Shao
authors.affiliation: Department of Urology, Jiangsu Province Geriatric Hospital 65 Jiangsu Road, Nanjing 210024, China.
authors.forename: Di
authors.initials: D
authors.lastname: Qiao
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road, Nanjing 210029, China.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Shanghai Institute of Dermatology Shanghai 200050, China.
authors.forename: Ningjing
authors.initials: N
authors.lastname: Song
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road, Nanjing 210029, China.
authors.forename: Ninghong
authors.initials: N
authors.lastname: Song
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010145:Paget Disease, Extramammary / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2
keywords: Extramammary Paget disease; VEGF; bFGF