title: Erythroivorensin: A novel anti-inflammatory diterpene from the root-bark of Erythrophleum ivorense (A Chev.).
abstract: The stem- and root-bark of Erythrophleum ivorense (A Chev., family, Fabaceae) are routinely employed in the West African traditional medicine to treat inflammation and a variety of other disease conditions. Although the chemistry and pharmacology of cassaine-type diterpene alkaloids isolated from the stem-bark of the plant are fairly established, the root-bark has not yet been investigated. In the present study, the crude aqueous-alcohol extract of the root-bark was demonstrated to display a time- and dose (30-300 mg/kg p.o.)-dependent anti-inflammatory effect in chicks. Comprehensive chromatographic analysis coupled with spectroscopic and X-ray study further allowed the assignment of one of the major anti-inflammatory constituents as a novel cassaine-type diterpene, erythroivorensin. The other major constituents were known anti-inflammatory compounds: a triterpene, betulinic acid and a flavonoid, eriodictyol. The dose (10-100mg/kg p.o.)-dependent anti-inflammatory effects of the three compounds were either comparable or more significant than the positive control, diclofenac.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy & Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
authors.forename: Francis A
authors.initials: FA
authors.lastname: Armah
authors.affiliation: Department of Herbal Medicine, KNUST, Kumasi, Ghana.
authors.forename: Kofi
authors.initials: K
authors.lastname: Annan
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy & Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
authors.forename: Abraham Y
authors.initials: AY
authors.lastname: Mensah
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy & Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
authors.forename: Isaac K
authors.initials: IK
authors.lastname: Amponsah
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London, UK.
authors.forename: Derek A
authors.initials: DA
authors.lastname: Tocher
authors.affiliation: Pharmacognosy Research Laboratories, Medway School of Science, University of Greenwich, Central Avenue, Chatham-Maritime, Kent ME4 4TB, UK. Electronic address: s.habtemariam@gre.ac.uk.
authors.forename: Solomon
authors.initials: S
authors.lastname: Habtemariam
mesh_terms: D045784:Abietanes / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification; D002645:Chickens; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification; D007887:Fabaceae / Q000737:chemistry*; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D045784:Abietanes; D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D010936:Plant Extracts; C000603144:erythroivorensin
keywords: Anti-inflammatory; Betulinic acid; Eriodictyol; Erythroivorensin; Erythrophleum ivorense; Fabaceae; Novel diterpene