title: Genetic and antigenic characterization of Bungowannah virus, a novel pestivirus.
abstract: Bungowannah virus, a possible new species within the genus Pestivirus, has been associated with a disease syndrome in pigs characterized by myocarditis with a high incidence of stillbirths. The current analysis of the whole-genome and antigenic properties of this virus confirms its unique identity, and further suggests that this virus is both genetically and antigenically remote from previously recognized pestiviruses. There was no evidence of reactivity with monoclonal antibodies (mAbs) that are generally considered to be pan-reactive with other viruses in the genus, and there was little cross reactivity with polyclonal sera. Subsequently, a set of novel mAbs has been generated which allow detection of Bungowannah virus. The combined data provide convincing evidence that Bungowannah virus is a member of the genus Pestivirus and should be officially recognized as a novel virus species.
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: peter.kirkland@dpi.nsw.gov.au.
authors.forename: P D
authors.initials: PD
authors.lastname: Kirkland
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: melinda.frost@dpi.nsw.gov.au.
authors.forename: M J
authors.initials: MJ
authors.lastname: Frost
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: katherine.king@dpi.nsw.gov.au.
authors.forename: K R
authors.initials: KR
authors.lastname: King
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: deborah.finlaison@dpi.nsw.gov.au.
authors.forename: D S
authors.initials: DS
authors.lastname: Finlaison
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: christine.hornitzky@dpi.nsw.gov.au.
authors.forename: C L
authors.initials: CL
authors.lastname: Hornitzky
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia.
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: maria.richter@fli.bund.de.
authors.forename: M
authors.initials: M
authors.lastname: Richter
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: ilona.reimann@fli.bund.de.
authors.forename: I
authors.initials: I
authors.lastname: Reimann
authors.affiliation: Department of Experimental Animal Facilities and Biorisk Management at the Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: malte.dauber@fli.bund.de.
authors.forename: M
authors.initials: M
authors.lastname: Dauber
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: horst.schirrmeier@fli.bund.de.
authors.forename: H
authors.initials: H
authors.lastname: Schirrmeier
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: martin.beer@fli.bund.de.
authors.forename: M
authors.initials: M
authors.lastname: Beer
authors.affiliation: National Animal Disease Centre, 2300 Dayton Avenue, Ames, IA, 50010, United States. Electronic address: julia.ridpath@ars.usda.gov.
authors.forename: J F
authors.initials: JF
authors.lastname: Ridpath
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D018182:Pestivirus Infections / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D000914:Antibodies, Viral
keywords: Antigenic characterisation; Bungowannah virus; Characterisation; Full-length genome sequence; Monoclonal antibodies; Pestivirus