title: Recombinant Lipoproteins as Novel Vaccines with Intrinsic Adjuvant.
abstract: A core platform technology for high production of recombinant lipoproteins with built-in immunostimulator for novel subunit vaccine development has been established. This platform technology has the following advantages: (1) easily convert antigen into lipidated recombinant protein using a fusion sequence containing lipobox and express high level (50-150mg/L) in Escherichia coli; (2) a robust high-yield up- and downstream bioprocess for lipoprotein production is successfully developed to devoid endotoxin contamination; (3) the lipid moiety of recombinant lipoproteins, which is identical to that of bacterial lipoproteins is recognized as danger signals by the immune system (Toll-like receptor 2 agonist), so both innate and adaptive immune responses can be induced by lipoproteins; and (4) successfully demonstrate the feasibility and safety of this core platform technology in meningococcal group B subunit vaccine, dengue subunit vaccine, novel subunit vaccine against Clostridium difficile-associated diseases, and HPV-based immunotherapeutic vaccines in animal model studies.
authors.affiliation: Vaccine R&D Center, National Health Research Institutes, Miaoli, Taiwan. Electronic address: pelechong@nhri.org.tw.
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
authors.affiliation: Vaccine R&D Center, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Huang
authors.affiliation: Vaccine R&D Center, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Leng
authors.affiliation: Vaccine R&D Center, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Shih-Jen
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: Vaccine R&D Center, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Hsin-Wei
authors.initials: HW
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic; D006801:Humans; D008074:Lipoproteins / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D008074:Lipoproteins; D014612:Vaccines
keywords: Dengue virus; Intrinsic adjuvant; Lipobox leader sequences; Lipoprotein; Meningococcal group B; Posttranslational modification; Subunit vaccine; Toll-like receptor