title: Effects of carbon nanofiber on physiology of Drosophila.
abstract: As nanomaterials are now widely utilized in a wide range of fields for both medical and industrial applications, concerns over their potential toxicity to human health and the environment have increased. To evaluate the toxicity of long-term exposure to carbon nanofibers (CNFs) in an in vivo system, we selected Drosophila melanogaster as a model organism. Oral administration of CNFs at a concentration of 1,000 μg/mL had adverse effects on fly physiology. Long-term administration of a high dose of CNFs (1,000 μg/mL) reduced larval viability based on the pupa:egg ratio, adult fly lifespan, reproductive activity, climbing activity, and survival rate in response to starvation stress. However, CNFs at a low concentration (100 μg/mL) did not show any significant deleterious effect on developmental rate or fecundity. Furthermore, long-term administration of a low dose of CNFs (100 μg/mL) increased lifespan and climbing ability, coincident with mild reactive oxygen species generation and stimulation of the antioxidant system. Taken together, our data suggest that a high dose of CNFs has obvious physiological toxicity, whereas low-dose chronic exposure to CNFs can actually have beneficial effects via stimulation of the antioxidant defense system.
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, Republic of Korea.
authors.forename: Shin-Hae
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, Republic of Korea.
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, Republic of Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Molecular Medicine, Graduate School of Medicine, Gachon University, Incheon, Republic of Korea.
authors.forename: Dongwoo
authors.initials: D
authors.lastname: Khang
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, Republic of Korea.
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Min
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D005298:Fertility / Q000187:drug effects; D007814:Larva / Q000187:drug effects; D008136:Longevity / Q000187:drug effects; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D036401:Toxicity Tests, Chronic
chemical_list: D017382:Reactive Oxygen Species; D002244:Carbon
keywords: Drosophila melanogaster; lifespan; reactive oxygen species; toxicity