title: Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction.
abstract: BACKGROUND
  Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as indicators for AMI.
  
  
  METHODS
  Detection of AMI patients and normal persons by using miRNA microarray chip analysis and miR-26a, miR-191, and miR-208b was screened out. Eighty-seven AMI patients and eighty-seven homogeneous healthy individuals were recruited. Total mRNA including miRNA was isolated and miR-26a, miR-191, and miR-208b expression were determined by qRT-PCR. Receiver operating characteristic curve analysis was performed to evaluate the instructive power of miR-26a, miR-191, and miR-208b for AMI. Dual-luciferase reporter assays indicated p21 is a direct target of miR-208b.
  
  
  RESULTS
  miR-26a and miR-191 were low expressed in AMI compared with normal healthy people, but miR-208b was expressed at a high level in AMI. miR-26a showed an area under the curve (AUC) of 0.745, with a sensitivity of 73.6 % and a specificity of 72.4 %.The AUC for miR-191 was 0.669, with a sensitivity of 62.1 % and a specificity of 69.0 %.The AUC for miR-208b was 0.674, with a sensitivity of 59.8 % and a specificity of 73.6 %.
  
  
  CONCLUSIONS
  miR-208b was significantly increased in the AMI compared with healthy people, while miR-26a and miR-191 were decreased. miR-26a, miR-191, and miR-208b were potential indices of AMI, and miR-208b was more effective in patients with non-ST-elevation myocardial infarction.
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. lichenchengzzu@163.com.
authors.forename: Chencheng
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, 450001, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Clinical Medicine, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, 450001, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Emergency, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*
chemical_list: D035683:MicroRNAs