title: Identification of Malassezia Species Isolated from Patients with Pityriasis Versicolor Using PCR-RFLP Method in Markazi Province, Central Iran.
abstract: BACKGROUND
  The lipophilic yeasts of Malassezia species are members of the normal skin microbial that are cause of pityriasis versicolor. Pityriasis versicolor is a common superficial fungal infection with world-wide distribution. The phenotypic methods for identification of Malassezia species usually are time consuming and unreliable to differentiate newly identified species. But DNA-based techniques rapidly and accurately identified Malassezia species. The purpose of this study was isolation and identification of Malassezia Species from patients with pityriasis versicolor by molecular methods in Markazi Province, Central Iran in 2012.
  
  
  METHODS
  Mycologic examinations including direct microscopy and culture were performed on clinical samples. DNA extraction was performed from colonies. The ITS1 region of rDNA from isolates of Malassezia species were amplified by PCR reaction. The PCR were digested by Cfo I enzyme.
  
  
  RESULTS
  From 70 skin samples, were microscopically positive for Malassezia elements, 60 samples were grown on culture medium (85.7%). Using PCR-RFLP method, that was performed on 60 isolates, 37(61.6%) M. globosa, 14(23.3%) M. furfur, 5(8.4%) M. sympodialis and 4(6.7%) M. restrictawere identified. In one case was isolated M. globosa along with M. restricta.
  
  
  CONCLUSIONS
  The PCR-RFLP method is a useful and reliable technique for identification of differentiation of Malas-sezia species.
authors.affiliation: 1. Molecular and Medicine Research Center, Arak University of Medical Sciences , Arak, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Didehdar
authors.affiliation: 2. Dept. of Medical Parasitology and Mycology, Army University of Medical Sciences , Tehran, Iran.
authors.forename: Amir Sayed Ali
authors.initials: AS
authors.lastname: Mehbod
authors.affiliation: 3. Dept. of Medical Parasitology and Mycology, Arak University of Medical Sciences , Arak, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Eslamirad
authors.affiliation: 3. Dept. of Medical Parasitology and Mycology, Arak University of Medical Sciences , Arak, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Mosayebi
authors.affiliation: 3. Dept. of Medical Parasitology and Mycology, Arak University of Medical Sciences , Arak, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Hajihossein
authors.affiliation: 3. Dept. of Medical Parasitology and Mycology, Arak University of Medical Sciences , Arak, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Ghorbanzade
authors.affiliation: 4. Dept. of Laboratory Science, Islamic Azad University of Arak , Arak, Iran.
authors.forename: Mahmoud Reza
authors.initials: MR
authors.lastname: Khazaei
keywords: Identification; Iran; Malassezia spp.; PCR-RFLP; Pityriasis versicolor