title: Characterisation and immunostimulatory activity of an α-(1→6)-d-glucan from the cultured Armillariella tabescens mycelia.
abstract: IPS-B2, an intracellular polysaccharide with anti-tumor effect, was isolated from cultured Armillariella tabescens mycelia by hot water extraction, anion-exchange and gel chromatography. Based on the results of paper chromatography (PC), gas chromatography (GC), infra-red (IR) spectroscopy and (13)C NMR, IPS-B2 was characterized as an α-(1→6)-d-glucan with a molecular weight of 49.5kDa. The effects of IPS-B2 on murine peritoneal macrophages were further investigated. The results demonstrated that IPS-B2 induced nitric oxide (NO) and cytokines (TNF-α, IL-1β and IL-6) production in macrophages. The outcomes of real-time reverse transcription-polymerase chain reaction (RT-PCR) proved that the transcribing level of inducible NO synthase (iNOS), TNF-α, IL-1β and IL-6 mRNA in the peritoneal macrophages have been augmented by IPS-B2. These data suggest that the anti-tumor activity of the polysaccharide from A. tabescens may due to activation of macrophage.
authors.affiliation: College of Life Sciences, Sichuan University, 610064 Sichuan, China; Sichuan Institute of Chinese Material Medica, Chengdu, 610041 Sichuan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Luo
authors.affiliation: Sichuan Institute of Chinese Material Medica, Chengdu, 610041 Sichuan, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Sichuan University, 610064 Sichuan, China; Sichuan Institute of Chinese Material Medica, Chengdu, 610041 Sichuan, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Sichuan University, 610064 Sichuan, China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Sichuan University, 610064 Sichuan, China; Soil and Fertilizer Institute, Sichuan Academy of agricultural Sciences, 610066 Sichuan, China. Electronic address: xuxiaoyan629@gmail.com.
authors.forename: Linyong
authors.initials: L
authors.lastname: Zheng
keywords: Armillariella tabescens; Cytokines; Macrophage; Nitric oxide; Polysaccharide