title: Evaluation of miR-29c inhibits endotheliocyte migration and angiogenesis of human endothelial cells by suppressing the insulin like growth factor 1.
abstract: MicroRNAs, a class of 22-nucleotide non-coding RNAs, modulate gene expression by associating with the 3'-untranslated regions (3'-UTRs) of messenger RNAs (mRNAs). Although multiple miRNAs are known to be regulated during angiogenesis, their individual roles in blood vessel development are still not fully understood. Herein, we investigate the role of miR-29c in regulating cell cycle and angiogenic phenotype of endothelial cells. The results showed that IGF-1 is highly expressed and down-regulated by miR-29c in human umbilical vein endothelial cells (HUVEC). Consistent with this preliminary finding, introduction of exogenous miR-29c or miR-29c inhibitor alters cell cycle progression, proliferation and tube formation of HUVEC, respectively. Furthermore, by using luciferase reporter assay, we find that the expression of IGF-1, a suppressor transcription factor, is directly regulated by miR-29c through 3'-UTR. In addition, we show that the selective inhibition of PI3K/AKT pathway prior to miR-29c stimulation prevents the expression of angiogenesis suppressor miRNAs that are family and cluster specific. As a conclusion, we find that miR-29c plays a significant role in regulating cell cycle, proliferation and angiogenic properties of HUVECs. This function is likely mediated through IGF-1 proteins at the post-transcriptional level. As a novel molecular target, miR-29c may have a potential value in the treatment of angiogenesis-associated diseases, such as cardiovascular diseases and cancers.
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Juhong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine Winston-Salem, NC, 27101, USA.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: West China Stomatological Hospital, Sichuan University Chengdu, Sichuan, 610041, China ; State Key Laboratory of Oral Diseases Chengdu, Sichuan, 610041, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tang
authors.affiliation: College of Stomatology, Chongqing Medical University Chongqing, 401147, China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing, 401147, China ; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing, 401147, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Zheng
keywords: IGF-1; angiogenesis; endothelial cell; miRNA-29c