title: Clinicopathological significance and biological role of TCF21 mRNA in breast cancer.
abstract: TCF21 is known to function as a tumor suppressor and deregulated in several types of cancers; however, its role in breast cancer remains poorly understood. The aim of this study was to examine the expression of TCF21 messenger RNA (mRNA) in breast cancer and evaluate its clinical significance and biological role in tumor progression. TCF21 mRNA expression was analyzed in breast cancer cell lines and tissues by qRT-PCR. Overexpression approach was used to investigate the biological functions of TCF21 mRNA in breast cancer cell line (MDA-MB-231). A notably lower level of TCF21 mRNA expression was found in breast cancer cell lines and tissues. Furthermore, the low expression of TCF21 mRNA was associated with large tumor size and positive lymph node metastasis. Functional analysis showed that overexpression of TCF21 mRNA inhibited cell proliferation and epithelial-mesenchymal transition (EMT) of MDA-MB-231. In conclusion, our data provided the first evidence that TCF21 mRNA is significantly downregulated in breast cancer cell lines and tissues and regulates breast cancer cell proliferation and EMT. Thus, TCF21 may act as a potential therapeutic target for breast cancer intervention.
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Ministry of Education; Department of Microbiology and Immunology, Medical School Southeast University, Nanjing, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Ministry of Education; Department of Microbiology and Immunology, Medical School Southeast University, Nanjing, Jiangsu, China.
authors.forename: Xueren
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China.
authors.forename: Mingxi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Ministry of Education; Department of Microbiology and Immunology, Medical School Southeast University, Nanjing, Jiangsu, China. zhjq@seu.edu.cn.
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D061986:MCF-7 Cells; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D012333:RNA, Messenger; C491825:TCF21 protein, human
keywords: Breast cancer; Proliferation; TCF21; Tumor suppressor; mRNA