title: A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours.
abstract: Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials.
authors.affiliation: Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.|Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Lies
authors.initials: L
authors.lastname: Bogaert
authors.affiliation: Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Woodham
authors.affiliation: Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.|Departments of Molecular Microbiology & Immunology and Obstetrics & Gynecology, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
authors.forename: Diane M
authors.initials: DM
authors.lastname: Da Silva
authors.affiliation: Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Ann
authors.initials: A
authors.lastname: Martens
authors.affiliation: Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Meyer
authors.affiliation: Departments of Molecular Microbiology & Immunology and Obstetrics & Gynecology, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.|Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
authors.forename: W Martin
authors.initials: WM
authors.lastname: Kast
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D006734:Horse Diseases / Q000517:prevention & control / Q000628:therapy / Q000821:virology*; D006736:Horses; D051379:Mice; D030361:Papillomavirus Infections / Q000517:prevention & control / Q000628:therapy / Q000662:veterinary* / Q000821:virology; D012878:Skin Neoplasms / Q000517:prevention & control / Q000628:therapy / Q000662:veterinary* / Q000821:virology