title: Impact of Atmospheric Microparticles on the Development of Oxidative Stress in Healthy City/Industrial Seaport Residents.
abstract: Atmospheric microsized particles producing reactive oxygen species can pose a serious health risk for city residents. We studied the responses of organisms to microparticles in 255 healthy volunteers living in areas with different levels of microparticle air pollution. We analyzed the distribution of microparticles in snow samples by size and content. ELISA and flow cytometry methods were employed to determine the parameters of the thiol-disulfide metabolism, peroxidation and antioxidant, genotoxicity, and energy state of the leukocytes. We found that, in the park areas, microparticles with a size of 800 μm or more were predominant (96%), while in the industrial areas, they tended to be less than 50 μm (93%), including size 200-300 nm (7%). In the industrial areas, we determined the oxidative modification of proteins (21% compared to the park areas, p ≤ 0.05) and DNA (12%, p ≤ 0.05), as well as changes in leukocytes' energy potential (53%, p ≤ 0.05). An increase in total antioxidant activity (82%, p ≤ 0.01) and thiol-disulfide system response (thioredoxin increasing by 33%, p ≤ 0.01; glutathione, 30%, p ≤ 0.01 with stable reductases levels) maintains a balance of peroxidation-antioxidant processes, protecting cellular and subcellular structures from significant oxidative damage.
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia ; Far Eastern Federal University, 8 Sukhanova Street, Vladivostok 690950, Russia.
authors.forename: Kirill
authors.initials: K
authors.lastname: Golokhvast
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Vitkina
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Gvozdenko
authors.affiliation: Far Eastern Center of Physiology and Pathology of Respiration, 22 Kalinina Street, Blagoveshchensk 675000, Russia.
authors.forename: Victor
authors.initials: V
authors.lastname: Kolosov
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia.
authors.forename: Vera
authors.initials: V
authors.lastname: Yankova
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kondratieva
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Gorkavaya
authors.affiliation: Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Nazarenko
authors.affiliation: Far Eastern Federal University, 8 Sukhanova Street, Vladivostok 690950, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chaika
authors.affiliation: Far Eastern Geological Institute FEB RAS, 159 Prospekt 100-letiya, Vladivostok 690022, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Romanova
authors.affiliation: Far Eastern Geological Institute FEB RAS, 159 Prospekt 100-letiya, Vladivostok 690022, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Karabtsov
authors.affiliation: Far Eastern Center of Physiology and Pathology of Respiration, 22 Kalinina Street, Blagoveshchensk 675000, Russia.
authors.forename: Juliy
authors.initials: J
authors.lastname: Perelman
authors.affiliation: Far Eastern Federal University, 8 Sukhanova Street, Vladivostok 690950, Russia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kiku
authors.affiliation: Department of Toxicology and Forensics, Medical School, University of Crete, Heraklion, 71300 Crete, Greece.
authors.forename: Aristidis
authors.initials: A
authors.lastname: Tsatsakis
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D005978:Glutathione / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D052638:Particulate Matter / Q000737:chemistry / Q000633:toxicity*; D012914:Snow / Q000737:chemistry; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052638:Particulate Matter; D013879:Thioredoxins; D004247:DNA; D005978:Glutathione