title: Preventive effects of oleuropein against cardiac remodeling after myocardial infarction in Wistar rat through inhibiting angiotensin-converting enzyme activity.
abstract: OBJECTIVE
  Myocardial infarction remains the major cause of global death due to cardiovascular diseases. This study aimed to assess the protective role of oleuropein in attenuating the cardiac remodeling in isoproterenol-induced myocardial infarction in rats.
  
  
  RESULTS
  Male Wistar rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with oleuropein at two different doses (20 and 40 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop+Oleu20) and (Isop+Oleu40) groups. The subcutaneous injection of isoproterenol (100 mg/kg body weight) to untreated rats for two consecutive days showed significant increases in ST-segment elevation, heart weight index and alteration in the ECG pattern and hemodynamic function. Else, serum levels of cardiac troponin-T, creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) underwent a notable rise in serum of Isop group by (345, 82, 73 and 106%, respectively) as compared to normal rats. Isoproterenol-induced myocardial injury was evidenced by alteration in serum lipids profile and increased activities of pancreatic lipase by 94% and angiotensin-converting enzyme (ACE) by 78% which reflects the occurrence of cardiac remodeling process. The histopathological findings of the infarcted group showed myocardium necrosis and cells inflammatory infiltration. However, the treatment with oleuropein gave a good protection of the myocardium by decreasing cardiac injury markers specially troponin-T, restoring hemodynamic parameters and attenuating cardiac remodeling process through inhibition of ACE activity.
  
  
  CONCLUSIONS
  Oleuropein offers high preventive effects from cardiac remodeling process in rats with acute myocardial infarction.
authors.affiliation: a Faculty of Sciences of Sfax , Laboratory of Animal Physiology, University of Sfax , Sfax , Tunisia .|b Department of Internal Medicine , Hospital of Sidi Bouzid , Sidi Bouzid , Tunisia .
authors.forename: Kais
authors.initials: K
authors.lastname: Mnafgui
authors.affiliation: c Faculty of Sciences of Sfax , Laboratory of Plant Biotechnology , Sfax , Tunisia .
authors.forename: Ines
authors.initials: I
authors.lastname: Khlif
authors.affiliation: b Department of Internal Medicine , Hospital of Sidi Bouzid , Sidi Bouzid , Tunisia .
authors.forename: Raouf
authors.initials: R
authors.lastname: Hajji
authors.affiliation: b Department of Internal Medicine , Hospital of Sidi Bouzid , Sidi Bouzid , Tunisia .
authors.forename: Fatma
authors.initials: F
authors.lastname: Derbali
authors.affiliation: d Faculty of Medicine of Monastir , Monastir , Tunisia .
authors.forename: Ftouma
authors.initials: F
authors.lastname: Kraiem
authors.affiliation: e Department of Cardiology , Centre Hospitalier Intercommunal Robert Ballanger , Aulnay-Sous-Bois , France .
authors.forename: Hedi
authors.initials: H
authors.lastname: Ellefi
authors.affiliation: f ICN, Université Nice Sophia Antipolis , Cedex , France .|g Laboratory of Pharmacognosy and Natural Products Chemistry , School of Pharmacy, University of Athens , Athens , Greece , and.
authors.forename: Thomas
authors.initials: T
authors.lastname: Michel
authors.affiliation: g Laboratory of Pharmacognosy and Natural Products Chemistry , School of Pharmacy, University of Athens , Athens , Greece , and.
authors.forename: Maria
authors.initials: M
authors.lastname: Halabalaki
authors.affiliation: g Laboratory of Pharmacognosy and Natural Products Chemistry , School of Pharmacy, University of Athens , Athens , Greece , and.
authors.forename: Alexios Leandros
authors.initials: AL
authors.lastname: Skaltsounis
authors.affiliation: a Faculty of Sciences of Sfax , Laboratory of Animal Physiology, University of Sfax , Sfax , Tunisia .
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: Elfeki
authors.affiliation: h Faculty of Sciences of Sfax , Laboratory of Chemistry of Natural Products , Sfax , Tunisia.
authors.forename: Neji
authors.initials: N
authors.lastname: Gharsallah
authors.affiliation: c Faculty of Sciences of Sfax , Laboratory of Plant Biotechnology , Sfax , Tunisia .
authors.forename: Noureddine
authors.initials: N
authors.lastname: Allouche
mesh_terms: D000318:Adrenergic beta-Agonists; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000627:therapeutic use*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004562:Electrocardiography / Q000187:drug effects; D006334:Heart Function Tests; D006439:Hemodynamics / Q000187:drug effects; D057888:Iridoid Glucosides; D039823:Iridoids / Q000302:isolation & purification / Q000627:therapeutic use*; D007545:Isoproterenol; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control*; D031658:Olea / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014665:Vasodilator Agents / Q000627:therapeutic use*; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: D000318:Adrenergic beta-Agonists; D000806:Angiotensin-Converting Enzyme Inhibitors; D057888:Iridoid Glucosides; D039823:Iridoids; D014665:Vasodilator Agents; C002769:oleuropein; D007545:Isoproterenol
keywords: ACE; ECG; cardiac remodeling; oleuropein; troponin-T