title: Comparison of the pathogenicity of the USDA challenge virus strain to a field strain of infectious laryngotracheitis virus.
abstract: Infectious laryngotracheitis virus (ILTV) causes respiratory disease in chickens. This alphaherpesvirus infects laryngeal tracheal epithelial cells and causes outbreaks culminating in decreases in egg production, respiratory distress in chickens and mortality. There are several different vaccines to combat symptoms of the virus, including chicken embryo origin, tissue culture origin and recombinant vaccines. All vaccines licensed for use in the U.S. are tested for efficacy and potency according to U.S. federal regulation using a vaccine challenge assay involving the use of an ILT challenge virus. This challenge virus is provided to biologics companies by the Center for Veterinary Biologics (CVB), United States Department of Agriculture (USDA). The current USDA challenge virus originated from a vaccine strain and has been subjected to multiple passages in eggs, and may not represent what is currently circulating in the field. The objective of this study was to evaluate and compare the pathogenicity of USDA's challenge virus strain to the pathogenicity of a recent ILT field isolate. Using the challenge virus and various dilutions of the field isolate, clinical signs, mortality and pathology were evaluated in chickens. Results indicate that the field isolate at a 1:20 dilution is comparable in pathogenicity to the USDA challenge virus at a 1:4 dilution, and that the ILTV field isolate is a viable candidate that could be used as a challenge virus when evaluating vaccine efficacy.
authors.affiliation: Center for Veterinary Biologics, USDA, APHIS, 1920 Dayton Avenue, Ames, IA, 50010, USA. Electronic address: danielle.m.koski@aphis.usda.gov.
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Koski
authors.affiliation: National Veterinary Services Laboratories, USDA, APHIS, 1920 Dayton Avenue, Ames, IA, 50010, USA. Electronic address: ann.s.predgen@aphis.usda.gov.
authors.forename: Ann S
authors.initials: AS
authors.lastname: Predgen
authors.affiliation: Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1802 University Boulevard, Ames, IA, 50011, USA.
authors.forename: Darrell W
authors.initials: DW
authors.lastname: Trampel
authors.affiliation: Center for Veterinary Biologics, USDA, APHIS, 1920 Dayton Avenue, Ames, IA, 50010, USA. Electronic address: sandra.k.conrad@aphis.usda.gov.
authors.forename: Sandra K
authors.initials: SK
authors.lastname: Conrad
authors.affiliation: Center for Veterinary Biologics, USDA, APHIS, 1920 Dayton Avenue, Ames, IA, 50010, USA. Electronic address: debra.r.narwold@aphis.usda.gov.
authors.forename: Debra R
authors.initials: DR
authors.lastname: Narwold
authors.affiliation: Center for Veterinary Biologics, USDA, APHIS, 1920 Dayton Avenue, Ames, IA, 50010, USA. Electronic address: joseph.r.hermann@aphis.usda.gov.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Hermann
mesh_terms: D000818:Animals; D002645:Chickens; D025921:Iltovirus / Q000276:immunology / Q000472:pathogenicity*; D014765:Viral Vaccines / Q000276:immunology; D014774:Virulence
chemical_list: D014765:Viral Vaccines
keywords: Avian herpesvirus; Challenge virus; Laryngotracheitis; Pathogenicity; Vaccine