title: Rapid and Easy Histological Evaluation of Alveolar Human Bone Quality at Dental Implant Sites Using a Nondecalcified Frozen Cryofilm Section Technique: A Technical Report.
abstract: BACKGROUND
  The evaluation of bone quality at the site of the alveolar bone for a dental implant is very important. This study presents an easy technique for direct evaluation of alveolar bone quality using nondecalcified cryofilm frozen sections on human alveolar bone core samples.
  
  
  METHODS
  Core samples harvested from alveolar bone were immediately frozen in cooled hexanen and slowly cut using a disposable tungsten carbide blade; the sliced sections were collected with adhesive cryofilms. Staining was performed using von toluidine blue and von Kossa for microscopic observations.
  
  
  RESULTS
  All core samples clearly showed bone structure components of cortical bone, trabecular bone, bone marrow, blood vessels, and bone-related cells.
  
  
  CONCLUSIONS
  These results suggest the efficacy of a nondecalcified cryofilm frozen section technique for histological observation of surgical implant sites.
authors.affiliation: *Assistant Professor, Department of Dentistry and Oral Surgery, Osaka Medical College, Takatsuki-city, Japan. †Lecturer, Department of Dentistry and Oral Surgery, Osaka Medical AU3 College, Takatsuki-city, Japan. ‡Professor and Chairman, Department of Dentistry and Oral Surgery, Osaka Medical College, Takatsuki-city, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujita
authors.forename: Miwa
authors.initials: M
authors.lastname: Kanou
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Takahashi-Nakagawa
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sunano
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kimura
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ueno
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology*; D016616:Cryoultramicrotomy / Q000379:methods*; D003758:Dental Implantation, Endosseous / Q000379:methods*; D015921:Dental Implants; D006801:Humans; D008334:Mandible / Q000033:anatomy & histology* / Q000098:blood supply / Q000166:cytology; D008437:Maxilla / Q000033:anatomy & histology* / Q000098:blood supply / Q000166:cytology; D008853:Microscopy / Q000379:methods
chemical_list: D015921:Dental Implants