title: Toxicodynamic mechanisms and monitoring of acaricide resistance in the two-spotted spider mite.
abstract: The two-spotted spider (Tetranychus urticae) is one of the most serious pests world-wide and has developed resistance to many types of acaricides. Various mutations on acaricide target site genes have been determined to be responsible for toxicodynamic resistance, and the genotyping and frequency prediction of these mutations can be employed as an alternative resistance monitoring strategy. A quantitative sequencing (QS) protocol was reported as a population-based genotyping technique, and applied for the determination of resistance allele frequencies in T. urticae field populations. In addition, a modified glass vial bioassay method (residual contact vial bioassay, RCV) was implemented as a rapid on-site resistance monitoring tool. The QS protocol, together with the RCV, would greatly facilitate monitoring of T. urticae resistance. Recent completion of T. urticae genome analysis should facilitate the identification of additional resistance genetic markers that can be employed for molecular resistance monitoring.
authors.affiliation: Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: jota486@snu.ac.kr.
authors.forename: Deok Ho
authors.initials: DH
authors.lastname: Kwon
authors.affiliation: Department of Veterinary & Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: J Marshall
authors.initials: JM
authors.lastname: Clark
authors.affiliation: Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Si Hyeock
authors.initials: SH
authors.lastname: Lee
mesh_terms: D056810:Acaricides / Q000633:toxicity*; D000818:Animals; D004351:Drug Resistance / Q000235:genetics*; D009154:Mutation; D040102:Tetranychidae / Q000187:drug effects / Q000235:genetics*
chemical_list: D056810:Acaricides
keywords: Quantitative sequencing; Residual contact vial bioassay; Resistance detection; Resistance monitoring; Tetranychus urticae; Toxicodynamic resistance mechanism