title: [Effect of Shen warming Pi strengthening method on the expression of serum T cell subsets in IBS-D rats].
abstract: OBJECTIVE
  To observe the effect of Shen warming Pi strengthening method on expressions of serum T cell subsets (C045+%, C03+%, and C04 +/COB+) in diarrhea-predominant irritable bowel syndrome (IBS-0) rats. Methods An IBS-0 rat model was established referring to AL-Chaer's modeling method combined with tail clamp and intragastric administration of sanna leaf. After modeling 30 SO rats were randomly divided into 6 groups according to random digit table, i.e., the model group, the high, middle, low dose Wenshen Jianpi Recipe (WJR) groups, and the Sishen Pill control group, 6 in each group. A normal control group consisting of 6 SO rats were also set up. Rats in high, middle, low dose WJ R groups were administered by gastrogavage with boil-free WJ R at the daily dose of 3. 100, 1. 550, 0. 775 g/kg, respectively. Rats in the Sis hen Pill control group were administered by gastrogavage with boil-free Sis hen Pill at the daily dose of 0. 736 g/kg. Equal volume of normal saline was given by gastrogavage to rats in the model group and the normal control group. All medication lasted for 2 successive weeks. Rats' general state, expressions of T cell subsets (CD45+%, CD3+%, and CD4+ /CDB+) changes were observed.
  
  
  RESULTS
  Compared with the normal control group, expressions of CD45+% and CD3+% increased, but CD4+ /CDB+ decreased with statistical difference (P < 0. 05). Compared with the model group, expressions of CD45+% and CD3+% decreased, but CD4+ ICDB+ increased with statistical difference in high, middle, low dose WJR groups, and the Sis hen Pill control group (P <0. 05). Compared with the Sis hen Pill control group, there was statistical difference in all indices except CD45+ value in the low dose SWPSM group (P <0. 05). Compared with the low dose WJ R group, the expression of CD3+% decreased in high and middle dose WJR groups, and the Sis hen Pill control group; CD4+ /CD8+ increased in the Sishen Pill control group and the high dose SWPSM group (all P < 0. 05).
  
  
  CONCLUSIONS
  WJR showed better treatment effect. The mechanism of Shen warming Pi strengthening method might be achieved by regulating expressions of CD45+% and CD3+%, and CD4+ /CD8+ ratios.
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Su
authors.forename: Ru-han
authors.initials: RH
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yi-bing
authors.initials: YB
authors.lastname: Bai
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Shi
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal; D005260:Female; D043183:Irritable Bowel Syndrome / Q000628:therapy*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D051381:Rats; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D017493:Leukocyte Common Antigens; C516771:Ptprc protein, rat