title: Immunodetection of P-selectin using an antibody to its C-terminal tag.
abstract: P-selectin is a multi-domain glycoprotein expressed on activated endothelial cells and activated platelets. We previously expressed a recombinant form of P-selectin containing only its N-terminal lectin and EGF domains in CHO-K1 cells and showed that these two domains are sufficient to mediate ligand binding. We have now expressed the same construct in CHO-Lec1 cells that make truncated glycans. The uniform glycosylation in these cells should make it easier to crystallize this protein.
authors.affiliation: Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73034, USA, padmaja.mehta.dsouza@omrf.org.
authors.forename: Padmaja
authors.initials: P
authors.lastname: Mehta-D'souza
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D002846:Chromatography, Affinity; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D019007:P-Selectin / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D016622:Silver Staining; D012995:Solubility
chemical_list: D000906:Antibodies; D019007:P-Selectin; D004815:Epidermal Growth Factor