title: Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures.
abstract: Validation of a high-throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial cancer cell lines compared to 2D culture.
authors.affiliation: Department of BioSciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Engel
authors.affiliation: Department of BioSciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
authors.forename: Pamela E
authors.initials: PE
authors.lastname: Constantinou
authors.affiliation: Department of BioSciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
authors.forename: Lindsey K
authors.initials: LK
authors.lastname: Sablatura
authors.affiliation: BioTime, Incorporated, 1301 Harbor Bay Parkway, Alameda, CA, 94502, USA.
authors.forename: Nathaniel J
authors.initials: NJ
authors.lastname: Doty
authors.affiliation: Department of BioSciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Carson
authors.affiliation: Department of BioSciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Farach-Carson
authors.affiliation: Department of BioSciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Harrington
authors.affiliation: BioTime, Incorporated, 1301 Harbor Bay Parkway, Alameda, CA, 94502, USA.
authors.forename: Thomas I
authors.initials: TI
authors.lastname: Zarembinski
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D001331:Automation; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004353:Drug Evaluation, Preclinical; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D018613:Microscopy, Confocal; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid
keywords: 3D culture; coculture; drug screening; high throughput; hyaluronic acid