title: Metalated nucleotide chemisorption on hydroxyapatite.
abstract: The experiments here reported evidence on the importance of the residual charge of a nucleotide derivative, for the adsorption on nHAP (hydroxyapatite nanocrystals), in water solution. We found that the simple presence of phosphates on the nucleotide derivative does not guarantee adsorption on nHAP. On the other hand, we demonstrated that a cationic or neutral charge on a nucleotide derivative produces a strongly reduced chemical adsorption (chemisorption) whereas, in the presence of a net negative charge, relevant adsorption on nHAP is observed. The number of phosphates can only modulate the adsorption efficiency of a molecule provided that this latter bears an overall negative charge. The neutral zwitterionic nucleotide Pt(II) complexes, bearing negatively charged phosphates, are unable to give stable chemisorption. Previous considerations are important to model the binding ability of phosphate bearing nucleotide derivatives or molecules on hydroxyapatite. The findings reported in the present paper could be relevant in bone tissue targeting or nHAP mediated drug delivery.
authors.affiliation: University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100, Lecce, Italy. Electronic address: michele.benedetti@unisalento.it.
authors.forename: Michele
authors.initials: M
authors.lastname: Benedetti
authors.affiliation: University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100, Lecce, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Antonucci
authors.affiliation: University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100, Lecce, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: De Castro
authors.affiliation: University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100, Lecce, Italy.
authors.forename: Chiara R
authors.initials: CR
authors.lastname: Girelli
authors.affiliation: University of Bologna, Department of Chemistry "G. Ciamician", Via Selmi 2, 40126, Bologna Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Lelli
authors.affiliation: University of Bologna, Department of Chemistry "G. Ciamician", Via Selmi 2, 40126, Bologna Italy.
authors.forename: Norberto
authors.initials: N
authors.lastname: Roveri
authors.affiliation: University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100, Lecce, Italy. Electronic address: fp.fanizzi@unisalento.it.
authors.forename: Francesco P
authors.initials: FP
authors.lastname: Fanizzi
mesh_terms: D000327:Adsorption; D017886:Durapatite / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D009696:Nucleic Acids / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D009696:Nucleic Acids; D009711:Nucleotides; D009944:Organoplatinum Compounds; D017886:Durapatite
keywords: Antitumor drug; Antiviral drug; Hydroxyapatite nanocrystals; Nanomaterials; Nucleotide adsorption; Platinum complex