title: Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity.
abstract: Sulfated polysaccharides (fucoidan) from brown seaweed Ascophyllum nodosum were extracted by microwave assisted extraction (MAE) technology. Different conditions of temperature (90-150°C), extraction time (5-30 min) were evaluated and optimal fucoidan yield was 16.08%, obtained from 120°C for 15 min's extraction. Compositional analysis, GPC, HPAEC and IR analysis were employed for characterization of extracted sulfated polysaccharides. Fucose was the main monosaccharide of fucoidan extracted at 90°C while glucuronic acid was the main monosaccharide of fucoidan extracted at 150°C. Both the molecular weight and sulfate content of extracted fucoidan increased with decreasing extraction temperature. All fucoidans exhibited antioxidant activities as measured by DPPH scavenging and reducing power, among which fucoidan extracted at 90°C was highest. This study shows that MAE is an efficient technology to extract sulfated polysaccharides from seaweed and Ascophyllum nodosum could potentially be a resource for natural antioxidants.
authors.affiliation: Green Chemistry Centre of Excellence, University of York, Heslington, YO10 5DD York, UK.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Green Chemistry Centre of Excellence, University of York, Heslington, YO10 5DD York, UK. Electronic address: duncan.macquarrie@york.ac.uk.
authors.forename: Duncan
authors.initials: D
authors.lastname: Macquarrie
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D044646:Ascophyllum / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D008872:Microwaves; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002241:Carbohydrates; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D014574:Uronic Acids; C007789:fucoidan; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Ascophyllum nodosum; Fucoidan; Microwave assisted extraction; Sulfated polysaccharides