title: Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections.
abstract: Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.
authors.forename: B Santhosh
authors.initials: BS
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Sandhyamani
authors.forename: Shaiju S
authors.initials: SS
authors.lastname: Nazeer
authors.forename: R S
authors.initials: RS
authors.lastname: Jayasree
mesh_terms: D005453:Fluorescence; D005557:Formaldehyde; D006801:Humans; D008168:Lung / Q000473:pathology*; D008856:Microscopy, Fluorescence; D016612:Paraffin Embedding; D038761:Photobleaching
chemical_list: D005557:Formaldehyde