title: The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells.
abstract: OBJECTIVE
  This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs).
  
  
  METHODS
  Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml(-1) or 1 mg ml(-1) with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity assays were used to calculate the attached DPSCs after 1 day of incubation. Water soluble tetrazolium assays were performed to investigate DPSCs proliferation on the treated dentine samples after three additional days of incubation. Two-way anova followed by Tukey-Kramer tests was used for statistical analyses (α = 0.05).
  
  
  RESULTS
  Dentine treated with 1 or 500 mg ml(-1) of DAP followed by EDTA caused significant increases in DPSCs attachment compared to the dentine treated with the DAP alone. The 500 mg ml(-1) of DAP with or without EDTA caused significant reductions in DPSCs proliferation. However, the treatment of dentine with 1 mg ml(-1) of DAP did not have significant negative effects on DPSCs proliferation regardless of the use of EDTA.
  
  
  CONCLUSIONS
  The use of 1 mg ml(-1) of DAP followed by 10 min of irrigation with EDTA in endodontic regeneration procedure may have no negative effects on the attachment and proliferation of DPSCs.
authors.affiliation: Department of Endodontics, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: Ki Wan
authors.initials: KW
authors.lastname: Kim
authors.affiliation: Division of Dental Biomaterials, Department of Restorative Dentistry, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: Ghaeth H
authors.initials: GH
authors.lastname: Yassen
authors.affiliation: Department of Endodontics, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: Ygal
authors.initials: Y
authors.lastname: Ehrlich
authors.affiliation: Department of Endodontics, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Spolnik
authors.affiliation: Division of Dental Biomaterials, Department of Restorative Dentistry, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Platt
authors.affiliation: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: L Jack
authors.initials: LJ
authors.lastname: Windsor
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D013234:Stem Cells / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004492:Edetic Acid
keywords: Dental pulp stem cells; double antibiotic paste; endodontic regeneration; ethylenediaminetetraacetic acid; radicular dentine