title: Balanced oxidative status by nesfatin-1 in intestinal ischemia-reperfusion.
abstract: OBJECTIVE
  Ischemia causes reversible or irreversible cell or tissue damage and reperfusion can exaggerate cellular damage. Microvascular dysfunction is induced and causes enhanced fluid filtration in capillaries. At the acute phase of reperfusion more oxygen radicals are activated. Nesfatin-1 protects brain against oxidative damage and heart against ischemia/reperfusion damage. In our study, we aimed to investigate the acute effect of chronic peripheral nesfatin-1 administration in intestinal ischemia/reperfusion created rats.
  
  
  METHODS
  Two-months-old, 28 Wistar Albino male rats, weighing an average of 200-250 g, were used and randomly divided into four experimental groups (n=7) as; Laparotomy, Ischemia/Reperfusion, Nesfatin-1+Laparotomy, Nesfatin-1+Ischemia/Reperfusion. Serum levels of total oxidant status (TOS) and total antioxidant status (TAS) were determined by colorimetric measurement method. The plasma levels of endotelin-1 and endothelial nitric oxide syntheses (eNOS) were analyzed by rat ELISA assay kits.
  
  
  RESULTS
  Plasma levels of endothelin-1 significantly increased, plasma level of eNOS, serum levels of TOS and TAS significantly decreased in nesfatin-1 applied groups. Additionally, The oxidative stress index (OSI) parameters decreased significantly in three groups compared to laparotomy.
  
  
  CONCLUSIONS
  Chronic peripheral nesfatin-1 administration can decrease eNOS level and OSI at the acute phase of ischemia/reperfusion. We suppose that it can be protective for ischemia/reperfusion injury by balancing oxidant capacity. On the other hand, this effect of nesfatin-1 is not related with micro-circular compensation and increases anti-oxidant capacity.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Ayada
authors.affiliation: Department of Thoracic Medicine, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Ümran
authors.initials: Ü
authors.lastname: Toru
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Genç
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Raziye
authors.initials: R
authors.lastname: Akcılar
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey.
authors.forename: Server
authors.initials: S
authors.lastname: Şahin
keywords: Nesfatin-1; eNOS; endothelin-1; ischemia/reperfusion; total antioxidant capacity; total oxidant capacity