title: Tracking single-particle rotation during macrophage uptake.
abstract: We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. The size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA. yy33@indiana.edu.
authors.forename: Lucero
authors.initials: L
authors.lastname: Sanchez
authors.forename: Paul
authors.initials: P
authors.lastname: Patton
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Anthony
authors.forename: Yi
authors.initials: Y
authors.lastname: Yi
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000818:Animals; D001692:Biological Transport; D007074:Immunoglobulin G / Q000378:metabolism; D008024:Ligands; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008863:Microspheres; D000067996:RAW 264.7 Cells; D012399:Rotation
chemical_list: D007074:Immunoglobulin G; D008024:Ligands