title: Invasion process of induced deep nodular endometriosis in an experimental baboon model: similarities with collective cell migration?
abstract: OBJECTIVE
  To determine the implications of collective cell migration in the invasion phenomenon observed in deep endometriotic lesions induced in a baboon model.
  
  
  METHODS
  Study of morphology and collective cell migration markers in invasive and noninvasive deep endometriotic lesions induced in a baboon model. Invasive lesions were defined as the presence of endometrial glands and stroma in surrounding organs, and a distinction was made between the center of the lesion (glands present in the main lesion) and the invasion front (glands present in surrounding organs).
  
  
  METHODS
  Academic research unit.
  
  
  METHODS
  Ten female baboons (Papio anubis).
  
  
  METHODS
  Recovery of induced deep nodular endometriotic nodules.
  
  
  METHODS
  Evaluation of the morphology of glands by analysis of noninvasive and invasive lesions (center of the lesion and invasion front); staining with specific antibodies (Ki67, E-cadherin, β-catenin) for immunohistochemical study of mitotic activity and cell-cell junctions.
  
  
  RESULTS
  Glands from invasive lesions, particularly from the invasion front, showed a significantly lower thickness coefficient, higher mitotic activity, and lower expression of E-cadherin and β-catenin than glands from noninvasive lesions and the center of invasive lesions.
  
  
  CONCLUSIONS
  We report altered morphology, increased mitotic activity, and fewer adhesion molecules in invasive glands present in induced nodular endometriosis, particularly along the invasion front, suggesting that collective cell migration is involved in the invasion process of deep endometriotic lesions induced in a baboon model.
authors.affiliation: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: Donnez
authors.affiliation: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Renan
authors.initials: R
authors.lastname: Orellana
authors.affiliation: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: Van Kerk
authors.affiliation: Chirurgie Expérimentale et Transplantation, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Dehoux
authors.affiliation: Infertility Research Unit, Brussels, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Donnez
authors.affiliation: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium. Electronic address: marie-madeleine.dolmans@uclouvain.be.
authors.forename: Marie-Madeleine
authors.initials: MM
authors.lastname: Dolmans
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D004195:Disease Models, Animal; D004715:Endometriosis / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D010215:Papio
keywords: Induced endometriosis; baboon model; cell-cell junction; collective cell migration; deep nodular endometriosis; invasion