title: MUC5B mucin production is upregulated by fibronectin and laminin in human lung epithelial cells via the integrin and ERK dependent pathway.
abstract: MUC5B mucin is a principal component of airway mucus and plays a key role in biodefense. We investigated the regulation of MUC5B production using the signals from extracellular matrix (ECM) components in NCI-H292 human lung epithelial cells. We found that MUC5B production in NCI-H292 cells cultured on fibronectin or laminin increased by 4-5-fold, with the increase occurring in a dose- and time-dependent manner. In contrast, MUC5B production was unchanged on type-IV collagen. Inhibition of integrin β1 induced upregulation of MUC5B and MUC5AC; however, inhibition of p38 MAPK did not show any remarkable change in overproduced MUC5B. Inhibition of extracellular signal-regulated kinase (ERK) pathway or the transcription factor NF-κB induced the recovery of overproduced MUC5B on fibronectin and laminin. These results suggest that MUC5B production can be regulated by ECM components and that MUC5B is upregulated by fibronectin and laminin via the integrin, ERK, and NF-κB dependent pathway.
authors.affiliation: a Faculty of Bioresource Sciences , Akita Prefectural University , Akita , Japan.
authors.forename: Yuho
authors.initials: Y
authors.lastname: Ito
authors.affiliation: a Faculty of Bioresource Sciences , Akita Prefectural University , Akita , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Iwashita
authors.affiliation: a Faculty of Bioresource Sciences , Akita Prefectural University , Akita , Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Kudoh
authors.affiliation: a Faculty of Bioresource Sciences , Akita Prefectural University , Akita , Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Kuramata
authors.affiliation: a Faculty of Bioresource Sciences , Akita Prefectural University , Akita , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Murata
mesh_terms: D024141:Collagen Type IV / Q000008:administration & dosage; D004847:Epithelial Cells / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019012:Integrin beta1 / Q000096:biosynthesis; D007797:Laminin / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000235:genetics; D055257:Mucin-5B / Q000096:biosynthesis* / Q000235:genetics; D009093:Mucus / Q000378:metabolism*; D012137:Respiratory System / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis
chemical_list: D024141:Collagen Type IV; D005353:Fibronectins; D019012:Integrin beta1; D007797:Laminin; C524578:MUC5B protein, human; D055257:Mucin-5B; D048051:p38 Mitogen-Activated Protein Kinases
keywords: MUC5AC; MUC5B; extracellular matrix; integrin; mucin