title: Anti-adipogenic activity of an olive seed extract in mouse fibroblasts.
abstract: The administration of different polyphenols protects against increased body weight and fat accumulation. The aim of the study was to determine the anti-adipogenic activity of an olive-seed polyphenolic extract, by means of mouse fibroblast cell line 3T3-L1 adipocyte differentiation.
  
  
  METHODS
  Cells were incubated and differentiated (6000 cells/cup) in the presence of olive-seed extract at 10 and 50 mg/l biosecure concentrations of polyphenols, and with no extract in the control sample. After 5 to 7 days mature adipocytes are formed. The fat clusters are quantified by means of red-oil staining, 490 nm absorbance, and the expression of the leptin and PPARg genes, and then compared to the values obtained in the cultures before and after adipocyte differentiation.
  
  
  RESULTS
  The control samples, with no extract, presented an accumulation of fat of 100%. By contrast, the addition of 50 mg/l of olive-seed extract polyphenols resulted in a 50% accumulation of fat, similar to that of the non-differentiated cells. A 10 mg/l extract concentration had no effect. Anti-adipogenic activity is thus confirmed, as the expression of the PPARg and leptin genes is reduced in adipocyte differentiation in the presence of extract at 50 mg/l. In conclusion, both the formation of fatty substances characteristic of adipogenesis, and the expression of the adipogenic PPARg and leptin genes are found to be inhibited by the prior addition of olive-seed extract polyphenols at a 50 mg/l concentration.
authors.affiliation: Laboratorio de Biotecnología y Proyectos, Biopartner S.L., Alcoy.. ernesto.cortes@umh.es.
authors.forename: Carmen
authors.initials: C
authors.lastname: Veciana-Galindo
authors.affiliation: Department of Pharmacology, Pediatrics and Organic Chemistry. Miguel Hernández University. San Juan de Alicante.. ernesto.cortes@umh.es.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Cortés-Castell
authors.affiliation: Laboratorio de Biotecnología y Proyectos, Biopartner S.L., Alcoy.. ernesto.cortes@umh.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Torró-Montell
authors.affiliation: Department of Clinical Medicine. Miguel Hernández University. San Juan de Alicante.. ernesto.cortes@umh.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Palazón-Bru
authors.affiliation: Laboratorio de Biotecnología y Proyectos, Biopartner S.L., Alcoy.. ernesto.cortes@umh.es.
authors.forename: Elia
authors.initials: E
authors.lastname: Sirvent-Segura
authors.affiliation: Department of Nursing and Nutrition. University of Alicante. San Vicente del Raspeig, Spain.. ernesto.cortes@umh.es.
authors.forename: María M
authors.initials: MM
authors.lastname: Rizo-Baeza
authors.affiliation: Department of Clinical Medicine. Miguel Hernández University. San Juan de Alicante.. ernesto.cortes@umh.es.
authors.forename: Vicente F
authors.initials: VF
authors.lastname: Gil-Guillén
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
chemical_list: D000960:Hypolipidemic Agents; D010936:Plant Extracts