title: Characterization of a Self-renewing and Multi-potent Cell Population Isolated from Human Minor Salivary Glands.
abstract: Adult stem cells play an important role in maintaining tissue homeostasis. Although these cells are found in many tissues, the presence of stem cells in the human minor salivary glands is not well explored. Using the explant culture method, we isolated a population of cells with self-renewal and differentiation capacities harboring that reside in the human minor salivary glands, called human minor salivary gland mesenchymal stem cells (hMSGMSCs). These cells show embryonic stem cell and mesenchymal stem cell phenotypes. Our results demonstrate that hMSGMSCs have the potential to undergo mesodermal, ectodermal and endodermal differentiation in conditioned culture systems in vitro. Furthermore, in vivo transplantation of hMSGMSCs into SCID mice after partial hepatectomy shows that hMSGMSCs are able to survive and engraft, characterized by the survival of labeled cells and the expression of the hepatocyte markers AFP and KRT18. These data demonstrate the existence of hMSGMSCs and suggest their potential in cell therapy and regenerative medicine.
authors.affiliation: Research Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.affiliation: 1] International Medical Plastic and Cosmetic Centre, China Meitan General Hospital, Beijing, PR China [2] Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cosmetic and Plastic Surgery, Evercare Beijing Medical &Beauty Hospital, Beijing, PR China.
authors.forename: Ming-juan
authors.initials: MJ
authors.lastname: Du
authors.affiliation: Microinvasive Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Xiang-yu
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Hai-zhou
authors.initials: HZ
authors.lastname: Tong
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Ting-lu
authors.initials: TL
authors.lastname: Han
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Wan-di
authors.initials: WD
authors.lastname: Li
authors.affiliation: Research Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Ning-bei
authors.initials: NB
authors.lastname: Yin
authors.affiliation: Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Ba Da Chu Road, Beijing, PR China.
authors.forename: Hai-dong
authors.initials: HD
authors.lastname: Li
authors.affiliation: 1] Research Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China [2] People's Hospital of Jincheng City, Jincheng, Shanxi, PR China.
authors.forename: Zhen-min
authors.initials: ZM
authors.lastname: Zhao
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D000066673:Cell Self Renewal / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D016513:Mice, SCID; D039902:Multipotent Stem Cells / Q000166:cytology*; D012470:Salivary Glands, Minor / Q000166:cytology*