title: Conformational Change Observed in the Active Site of Class C β-Lactamase MOX-1 upon Binding to Aztreonam.
abstract: We solved the crystal structure of the class C β-lactamase MOX-1 complexed with the inhibitor aztreonam at 1.9Å resolution. The main-chain oxygen of Ser315 interacts with the amide nitrogen of aztreonam. Surprisingly, compared to that in the structure of free MOX-1, this main-chain carboxyl changes its position significantly upon binding to aztreonam. This result indicates that the interaction between MOX-1 and β-lactams can be accompanied by conformational changes in the B3 β-strand main chain.
authors.affiliation: Department of Science, Yamagata University, Yamagata, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Oguri
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ishii
authors.affiliation: Department of Science, Yamagata University, Yamagata, Japan Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan ibuka@nupals.ac.jp.
authors.forename: Akiko
authors.identifier: http://orcid.org/0000-0002-5310-9216
authors.initials: A
authors.lastname: Shimizu-Ibuka
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001398:Aztreonam / Q000737:chemistry* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000648:ultrastructure*; D001665:Binding Sites; D002245:Carbon Dioxide / Q000737:chemistry; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D009070:Moxalactam / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000494:pharmacology; D011487:Protein Conformation; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000235:genetics / Q000648:ultrastructure*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002245:Carbon Dioxide; C041069:carboxyl radical; D001618:beta-Lactamases; C000601044:beta-lactamase Mox-1, Klebsiella pneumoniae; D001398:Aztreonam; D009070:Moxalactam