title: Nucleic acid quantification using nicking-displacement, rolling circle amplification and bio-bar-code mediated triple-amplification.
abstract: In the present study, an inductively-coupled plasma-mass spectrometry (ICP-MS)-based triple-amplification system, by combination of nicking-displacement, rolling circle amplification (RCA) and bio-bar-code probes, was fabricated for the detection of DNA target. By using this system, hepatitis B virus (HBV) DNA target down to 3.2×10(-17)M was detected by DNA probes labeled with Au nanoparticles (AuNPs). Single nucleotide polymorphisms in genes can also be effectively discriminated. In addition, we proved that this strategy is capable of detecting the target in complicated biological samples and holds great potential application in biomedical research.
authors.affiliation: School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China. Electronic address: xuemei_li@yeah.net.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Li
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China; State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Ning-Bo
authors.initials: NB
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Wei
mesh_terms: D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D006046:Gold / Q000737:chemistry; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D006046:Gold
keywords: Bio-bar-code; Hepatitis B virus; Inductively-coupled plasma-mass spectrometry; Rolling circle amplification; Triple-amplification