title: Impact of membrane-associated hydrogenases on the F₀F₁-ATPase in Escherichia coli during glycerol and mixed carbon fermentation: ATPase activity and its inhibition by N,N'-dicyclohexylcarbodiimide in the mutants lacking hydrogenases.
abstract: Escherichia coli is able to ferment glycerol and to produce molecular hydrogen (H2) by four membrane-associated hydrogenases (Hyd) changing activity in response to different conditions. In this study, overall ATPase activity of glycerol alone and mixed carbon sources (glucose and glycerol) fermented E. coli wild type and different Hyd mutants and its inhibition by N,N'-dicyclohexylcarbodiimide (DCCD) were first investigated. ATPase activity was higher in glycerol fermented wild type cells at pH 7.5 compared to pH 6.5 and pH 5.5; DCCD inhibited markedly ATPase activity at pH 7.5. The ATPase activity at pH 7.5, compared with wild type, was lower in selC and less in hypF single mutants, suppressed in hyaB hybC selC triple mutant. Moreover, total ATPase activity of mixed carbon fermented wild type cells was maximal at pH 7.5 and lowered at pH 5.5. The ATPase activities of hypF and hyaB hybC selC mutants were higher at pH 5.5, compared with wild type; DCCD inhibited markedly ATPase activity of hypF mutant. These results demonstrate that in E. coli during glycerol fermentation the membrane proton-translocating FOF1-ATPase has major input in overall ATPase activity and alkaline pH is more optimal for the FOF1-ATPase operation. Hyd-1 and Hyd-2 are required for the FOF1-ATPase activity upon anaerobic fermentation of glycerol. The impact of Hyd-1 and Hyd-2 on the FOF1-ATPase is more obvious during mixed carbon fermentation at slightly acidic pH.
authors.affiliation: Department of Microbiology & Plants and Microbes Biotechnology, Faculty of Biology, Yerevan State University, 1 A. Manoukian Str., Yerevan 0025, Armenia.
authors.forename: Syuzanna
authors.initials: S
authors.lastname: Blbulyan
authors.affiliation: Department of Microbiology & Plants and Microbes Biotechnology, Faculty of Biology, Yerevan State University, 1 A. Manoukian Str., Yerevan 0025, Armenia. Electronic address: Trchounian@ysu.am.
authors.forename: Armen
authors.initials: A
authors.lastname: Trchounian
mesh_terms: D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006864:Hydrogenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D004024:Dicyclohexylcarbodiimide; D010088:Oxidoreductases; C029746:uptake hydrogenase; D006864:Hydrogenase; D006180:Proton-Translocating ATPases; D005947:Glucose; D005990:Glycerol
keywords: Escherichia coli; F(O)F(1)-ATPase; Fermentation; Hydrogenases; Membrane proteins; Mutants