title: Bone defect regeneration and cortical bone parameters of type 2 diabetic rats are improved by insulin therapy.
abstract: Zucker Diabetic Fatty (ZDF) rats represent an established model of type 2 diabetes mellitus (T2DM) and display several features of human diabetic bone disease, including impaired osteoblast function, decreased bone strength, and delayed bone healing. Here, we determined whether glycemic control by insulin treatment prevents skeletal complications associated with diabetes. Subcritical femur defects were created in diabetic (fa/fa) and non-diabetic (+/+) ZDF rats. Diabetic rats were treated once daily with long-lasting insulin glargin for 12weeks for glycemic control. Insulin treatment successfully maintained serum levels of glycated hemoglobin, while untreated diabetic rats showed a 2-fold increase. Trabecular and cortical bone mass measured by μCT were decreased in diabetic rats. Insulin treatment increased bone mass of the cortical, but not of the trabecular bone compartment. Dynamic histomorphometry revealed a lower bone formation rate at the trabecular and periosteal cortical bone in diabetic animals and decreased serum procollagen type 1 N-terminal propeptide (P1NP, -49%) levels. Insulin treatment partially improved these parameters. In T2DM, serum levels of tartrate-resistant acid phosphatase (TRAP, +32%) and C-terminal telopeptide (CTX, +49%) were increased. Insulin treatment further elevated TRAP levels, but did not affect CTX levels. While diabetes impaired bone defect healing, glycemic control with insulin fully reversed these negative effects. In conclusion, insulin treatment reversed the adverse effects of T2DM on bone defect regeneration in rats mainly by improving osteoblast function and bone formation. This article is part of a Special Issue entitled Bone and diabetes.
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolic Bone Diseases, Department of Medicine III, Technische Universität Dresden, Germany.
authors.forename: A-K
authors.initials: AK
authors.lastname: Picke
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolic Bone Diseases, Department of Medicine III, Technische Universität Dresden, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Gordaliza Alaguero
authors.affiliation: Section Biomedical Imaging, MOIN CC, Department of Radiology and Neuroradiology, Christian-Albrechts-Universität zu Kiel, Germany.
authors.forename: G M
authors.initials: GM
authors.lastname: Campbell
authors.affiliation: Section Biomedical Imaging, MOIN CC, Department of Radiology and Neuroradiology, Christian-Albrechts-Universität zu Kiel, Germany.
authors.forename: C-C
authors.initials: CC
authors.lastname: Glüer
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolic Bone Diseases, Department of Medicine III, Technische Universität Dresden, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Salbach-Hirsch
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolic Bone Diseases, Department of Medicine III, Technische Universität Dresden, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Rauner
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolic Bone Diseases, Department of Medicine III, Technische Universität Dresden, Germany; DFG Research Center and Cluster of Excellence for Regenerative Therapies, Technische Universität Dresden, Germany.
authors.forename: L C
authors.initials: LC
authors.lastname: Hofbauer
authors.affiliation: Department of Orthopedics, Technische Universität Dresden, Germany. Electronic address: Christine.Hofbauer@uniklinikum-dresden.de.
authors.forename: C
authors.initials: C
authors.lastname: Hofbauer
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects* / Q000502:physiology; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D007328:Insulin / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D011924:Rats, Zucker
chemical_list: D007328:Insulin
keywords: Bone defect regeneration; Bone loss; Insulin; Osteoblast; Type 2 diabetes mellitus