title: Crystallization and preliminary X-ray diffraction analysis of the two distinct types of zebrafish β2-microglobulin.
abstract: β(2)-Microglobulin (β(2)m) noncovalently associates with the heavy chain of major histocompatibility complex class I (MHC I) molecules, which bind foreign antigen peptides to control the cytotoxic T lymphocyte (CTL) immune response. In contrast to mammals, there are distinct types of β(2)ms derived from two loci in a number of teleost species. In order to clarify the structures of the β(2)ms, the zebrafish (Danio rerio) β(2)ms Dare-β(2)m-I and Dare-β(2)m-II were expressed in Escherichia coli, purified and crystallized, and diffraction data were collected to 1.6 and 1.9 Å resolution, respectively. Both crystals belonged to space group P2(1)2(1)2(1). The unit-cell parameters were determined to be a = 38.2, b = 50.4, c = 50.9 Å for Dare-β(2)m-I and a = 38.9, b = 52.7, c = 65.8 Å for Dare-β(2)m-II. Each asymmetric unit was constituted of one molecule, with Matthews coefficients of 2.22 and 3.01 Å(3) Da(-1) and solvent contents of 45 and 59% for Dare-β(2)m-I and Dare-β(2)m-II, respectively. These two β(2)m structures will provide relevant information for further studies of the structures of the MHC I complex.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Zhaosan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Mansoor
authors.initials: M
authors.lastname: Tariq
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Junya
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D014961:X-Ray Diffraction; D015027:Zebrafish / Q000235:genetics* / Q000276:immunology; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D029941:Fish Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin
keywords: MHC class I; zebrafish; β2-microglobulin