title: [Spermidine enhances osteogenic differentiation and inhibits adipogenic differentiation of bone marrow-derived mesenchymal stem cells from ovariectomized mice].
abstract: OBJECTIVE
  To investigate the effects of spermidine (SPD) on the differentiation ability of bone marrow-derived mesenchymal stem cells (BMMSCs) from estrogen-deficient mouse models.
  
  
  METHODS
  Female C57/BL6J mice were randomly divided into sham group and ovariectomized (OVX) group. Two months after modeling, BMMSCs of sham and OVX mice were harvested and cultured, and then Western blotting was used to detect the expressions of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), peroxisome proliferator activated receptor gamma (PPARγ) and lipoprtein lipase (LPL). Mineralized nodules and lipid droplet formation ability of the BMMSCs from sham and OVX mice were evaluated and compared by alizarin red staining and oil red O staining, respectively. After BMMSCs from the OVX mice were cultured in osteogenesis-induced medium and adipogenesis-induced medium supplemented with SPD, their osteogenic and adipogenic differentiation capacities were evaluated by Western blotting and reverse transcription PCR to detect the expressions of Runx2, ALP, PPARγ and LPL, and the mineralized nodules and lipid droplet formation ability were examined by alizarin red staining and oil red O staining, respectively.
  
  
  RESULTS
  BMMSCs from OVX mice showed lower ability of osteogenic differentiation and higher ability of adipogenic differentiation compared to the ones from sham mice. SPD strengthened the ability of osteogenic differentiation and depressed the ability of adipogenic differentiation of BMMSCs from OVX mice compared with the BMMSCs without SPD intervention.
  
  
  CONCLUSIONS
  SPD enhances osteogenic differentiation and inhibits adipogenic differentiation of BMMSCs from OVX mice.
authors.affiliation: State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis / Q000187:drug effects*; D010052:Ovariectomy; D013095:Spermidine / Q000494:pharmacology*
chemical_list: D013095:Spermidine