title: BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.
abstract: BACKGROUND
  Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
  
  
  RESULTS
  We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.
  
  
  METHODS
  Software implemented in Python and datasets available for download from http://busco.ezlab.org.
  
  
  BACKGROUND
  evgeny.zdobnov@unige.ch
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Felipe A
authors.initials: FA
authors.lastname: Simão
authors.affiliation: Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Waterhouse
authors.affiliation: Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Ioannidis
authors.affiliation: Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Evgenia V
authors.initials: EV
authors.lastname: Kriventseva
authors.affiliation: Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Evgeny M
authors.initials: EM
authors.lastname: Zdobnov
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D018628:Gene Dosage / Q000235:genetics*; D016678:Genome; D023281:Genomics / Q000379:methods*; D006801:Humans; D058977:Molecular Sequence Annotation / Q000379:methods*; D012984:Software