title: Calea zacatechichi dichloromethane extract exhibits antidiarrheal and antinociceptive effects in mouse models mimicking irritable bowel syndrome.
abstract: Calea zacatechichi Schltdl. (Asteraceae alt. Compositae) is a Mexican plant commonly used in folk medicine to treat respiratory and gastrointestinal (GI) disorders. The objective of this study is to characterize the effect of C. zacatechichi extracts in mouse models mimicking the symptoms of irritable bowel syndrome (IBS). Powdered C. zacatechichi herb (leaves, stems, and flowers) was extracted with methanol. Methanolic extract was filtered and evaporated giving methanolic fraction. The residue was extracted with dichloromethane (DCM). Methanolic and DCM (200 mg/kg, per os) extracts were screened for their effect on GI motility in several in vitro tests, and the antidiarrheal and antinociceptive effects were assessed using mouse models. The influence of the DCM extract on motoric parameters and exploratory behaviors was also assessed. Finally, the composition of C. zacatechichi DCM extract was qualitatively analyzed using liquid chromatography-mass spectrometry (LC-MS) method. C. zacatechichi DCM extract significantly inhibited the contractility of mouse colon in vitro (IC50 = 17 ± 2 μg/ml). Administration of the DCM extract in vivo (200 mg/kg, per os) significantly prolonged the time of whole GI transit (46 ± 1 vs. 117 ± 27 min for control and DCM-treated animals, respectively; P = 0.0023), inhibited hypermotility, and reduced pain in mouse models mimicking functional GI disorders. Our findings suggest that constituents of the C. zacatechichi DCM extract exhibit antidiarrheal and analgesic activity. The extract may thus become an attractive material for isolation of compounds that may be used as a supplementary treatment for pain and diarrhea associated with IBS in the future.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Mazowiecka 6/8, 92-215, Lodz, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Sałaga
authors.forename: A
authors.initials: A
authors.lastname: Kowalczuk
authors.forename: M
authors.initials: M
authors.lastname: Zielinska
authors.forename: A
authors.initials: A
authors.lastname: Błażewicz
authors.forename: J
authors.initials: J
authors.lastname: Fichna
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000930:Antidiarrheals / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D019659:Asteraceae / Q000737:chemistry*; D002853:Chromatography, Liquid; D003967:Diarrhea / Q000188:drug therapy* / Q000209:etiology; D004195:Disease Models, Animal; D005772:Gastrointestinal Transit / Q000187:drug effects; D020128:Inhibitory Concentration 50; D043183:Irritable Bowel Syndrome / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D013058:Mass Spectrometry; D008519:Medicine, Traditional; D008800:Mexico; D051379:Mice; D008810:Mice, Inbred C57BL; D010146:Pain / Q000188:drug therapy / Q000209:etiology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D000930:Antidiarrheals; D010936:Plant Extracts