title: Circulating small non-coding RNA signature in head and neck squamous cell carcinoma.
abstract: The Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common human cancer, causing 350,000 individuals die worldwide each year. The overall prognosis in HNSCC patients has not significantly changed for the last decade. Complete understanding of the molecular mechanisms in HNSCC carcinogenesis could allow an earlier diagnosis and the use of more specific and effective therapies. In the present study we used deep sequencing to characterize small non-coding RNAs (sncRNAs) in serum from HNSCC patients and healthy donors. We identified, for the first time, a multi-marker signature of 3 major classes of circulating sncRNAs in HNSCC, revealing the presence of circulating novel and known miRNAs, and tRNA- and YRNA-derived small RNAs that were significantly deregulated in the sera of HNSCC patients compared to healthy controls. By implementing a triple-filtering approach we identified a subset of highly biologically relevant miRNA-mRNA interactions and we demonstrated that the same genes/pathways affected by somatic mutations in cancer are affected by changes in the abundance of miRNAs. Therefore, one important conclusion from our work is that during cancer development, there seems to be a convergence of oncogenic processes driven by somatic mutations and/or miRNA regulation affecting key cellular pathways.
authors.affiliation: University of Central Florida, Burnett School of Biomedical Sciences, College of Medicine Orlando, FL, USA.
authors.forename: Berta
authors.initials: B
authors.lastname: Victoria Martinez
authors.affiliation: Department of Biochemistry, University of California at Riverside, Riverside, CA, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Dhahbi
authors.affiliation: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA.
authors.forename: Yury O
authors.initials: YO
authors.lastname: Nunez Lopez
authors.affiliation: Deptartment of Cancer Genetics, Greater Poland Cancer Centre, Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Lamperska
authors.affiliation: Department of Biology and Environmental Studies, Poznan University of Medical Sciences, Poznan, Poland.|Department of Head and Neck Surgery, Greater Poland Cancer Centre, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Golusinski
authors.affiliation: Department of Head and Neck Surgery, Greater Poland Cancer Centre, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Luczewski
authors.affiliation: Deptartment of Cancer Genetics, Greater Poland Cancer Centre, Poznan, Poland.|Postgraduate School of Molecular Medicine, Medical University of Warsaw, Warszawa, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kolenda
authors.affiliation: Department of Medical Education, California Northstate University, Elk Grove, CA, USA.
authors.forename: Hani
authors.initials: H
authors.lastname: Atamna
authors.affiliation: Department of Biochemistry, University of California at Riverside, Riverside, CA, USA.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Spindler
authors.affiliation: Department of Head and Neck Surgery, Greater Poland Cancer Centre, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Golusinski
authors.affiliation: University of Central Florida, Burnett School of Biomedical Sciences, College of Medicine Orlando, FL, USA.|Department of Head and Neck Surgery, Greater Poland Cancer Centre, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Michal M
authors.initials: MM
authors.lastname: Masternak
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005260:Female; D006258:Head and Neck Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D022661:RNA, Untranslated / Q000097:blood* / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D022661:RNA, Untranslated
keywords: circulating small RNAs; head and neck cancer; microRNAs; next-generation sequencing; tRNA halves