title: [Effect of low-frequency electroacupuncture intervention on oxidative stress and glucose metabolism in rats with polycystic ovary syndrome].
abstract: OBJECTIVE
  To observe the effect of low-frequency electroacupuncture (EA) treatment on oxidative stress and metabolism disorder in polycystic ovary syndrome (PCOS) rats, so as to study its underlying mechanisms in improving PCOS.
  
  
  METHODS
  Thirty female SD rats were randomized into control group, model group and EA group (n = 10 in each group). The PCOS model was established by subcutaneous injection of testosterone propionate (1 mg/100 g, 10 mg/mL) for 8 weeks. EA (2 Hz) was applied to "Zhongwan" (CV 12) , "Guanyuan" (CV 4) and bilateral "Sanyinjiao" (SP 6) or "Housanli" (ST 36) for 30 min, once daily (except weekends) for 5 weeks. Serum testosterone (T) , sex hormone-binding globulin (SHBG) and fasting serum insulin (FINS) contents were detected by ELISA, serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assayed by thiobarbituric acid (TBA) and xanthine oxidation (XTO) methods, respectively, and fasting blood glucose (FBG) was determined by roche glucose meter. In addition, homeostasis model assessment for insulin resistance (HOMA-IR) and modified B cell function index (MBCI) were calculated.
  
  
  RESULTS
  Compared with the control group, serum T, FBG, FINS, HOMA-IR, and serum MDA levels were significantly increased in the model group (P < 0.01), while serum SHBG, MBCI and SOD levels were considerably decreased in the model group (P < 0.01). Following EA treatment, all the increased serum T, FBG, FINS, HOMA-IR, and serum MDA levels, and the decreased serum SHBG, MBCI and SOD levels were reversed obviously (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  EA treatment may normalize insulin sensitivity, ameliorate insulin resistance and hyperinsulinemia in PCOS rats, probably by regulating the function of pancreatic islets β cell and by reducing oxidative stress and free androgen.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zheng
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Dan-Feng
authors.initials: DF
authors.lastname: Ye
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Mao-Hua
authors.initials: MH
authors.lastname: Lai
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Ma
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D015671:Electroacupuncture; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008315:Malondialdehyde