title: Simple, Rapid, and Selective Isolation of 2S Albumins from Allergenic Seeds and Nuts.
abstract: The 2S albumins belong to the group of seed storage proteins present in different seeds and nuts. Due to their pronounced allergenic potential, which is often associated with severe allergic reactions, this protein family is of special interest in the field of allergen research. Here we present a simple, rapid, and selective method for the purification of 2S albumins directly from allergenic seeds and nuts. We systematically optimized the parameters "buffer system", "extraction temperature", "buffer molarity", and "pH " and were able to achieve 2S albumin purities of about 99% without further purification and demonstrate transferability of this method to nine different allergenic food matrices. Compared to conventional isolation routines, significant reduction of hands-on time and required laboratory equipment is achieved, but nonetheless higher protein yields are obtained. The presented method allows for the rapid purification of different 2S albumins including the corresponding isoforms from natural material.
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Marlene
authors.initials: M
authors.lastname: Hummel
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Wigger
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Tessa
authors.initials: T
authors.lastname: Höper
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Imke
authors.initials: I
authors.lastname: Westkamp
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Brockmeyer
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry / Q000302:isolation & purification*; D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D052179:Antigens, Plant / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D009754:Nuts / Q000737:chemistry* / Q000276:immunology
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant
keywords: 2S albumins; SDS-PAGE; allergens; extraction; isolation; nuts; purification; seed storage proteins; seeds