title: Effect of short-term cadmium stress on Populus nigra L. detached leaves.
abstract: Pollution by toxic metals, accumulating into soils as result of human activities, is a worldwide major concern in industrial countries. Plants exhibit different degrees of tolerance to heavy metals, as a consequence of their ability to exclude or accumulate them in particular tissues, organs or sub-cellular compartments. Molecular information about cellular processes affected by heavy metals is still largely incomplete. As a fast-growing, highly tolerant perennial plant species, poplar has become a model for environmental stress response investigations. To study the short-term effects of cadmium accumulation in leaves, we analyzed photosystem II (PSII) quantum yield, hydrogen peroxide (H2O2) generation, hormone levels variation, as well as proteome profile alteration of 50μM CdSO4 vacuum-infiltrated poplar (Populus nigra L.) detached leaves. Cadmium management brought about an early and sustained production of hydrogen peroxide, an increase of abscisic acid, ethylene and gibberellins content, as well as a decrease in cytokinins and auxin levels, whereas photosynthetic electron transport was unaffected. Proteomic analysis revealed that twenty-one proteins were differentially induced in cadmium-treated leaves. Identification of fifteen polypeptides allowed to ascertain that most of them were involved in stress response while the remaining ones were involved in photosynthetic carbon metabolism and energy production.
authors.affiliation: Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Pesche Italy.
authors.forename: Tonia
authors.initials: T
authors.lastname: Lomaglio
authors.affiliation: Dipartimento per la Biologia, Geologia e l'Ambiente, Università del Sannio, Benevento, Italy.
authors.forename: Mariapina
authors.initials: M
authors.lastname: Rocco
authors.affiliation: Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Pesche Italy.
authors.forename: Dalila
authors.initials: D
authors.lastname: Trupiano
authors.affiliation: Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Pesche Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: De Zio
authors.affiliation: Dipartimento di Biologia, Università di Roma Tor Vergata, Roma, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Grosso
authors.affiliation: Dipartimento di Biologia, Università di Roma Tor Vergata, Roma, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Marra
authors.affiliation: Dipartimento di Agricoltura, Ambientee Alimenti, Università degli Studi del Molise, Campobasso, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Delfine
authors.affiliation: Dipartimento di Biotecnologia e Scienze della Vita, Università dell' Insubria, Varese, Italy.
authors.forename: Donato
authors.initials: D
authors.lastname: Chiatante
authors.affiliation: Université d'Orléans, INRA, LBLGC, EA 1207, Laboratoire de Biologie des Ligneux et des Grandes Cultures, Orléans, France.
authors.forename: Domenico
authors.initials: D
authors.lastname: Morabito
authors.affiliation: Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Pesche Italy. Electronic address: scippa@unimol.it.
authors.forename: Gabriella Stefania
authors.initials: GS
authors.lastname: Scippa
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism*; D003583:Cytokinins / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D005030:Ethylenes / Q000378:metabolism; D005875:Gibberellins / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D032107:Populus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D020543:Proteome; D013312:Stress, Physiological
chemical_list: D003583:Cytokinins; D005030:Ethylenes; D005875:Gibberellins; D007210:Indoleacetic Acids; D045332:Photosystem II Protein Complex; D010937:Plant Growth Regulators; D020543:Proteome; D002104:Cadmium; D000040:Abscisic Acid; C036216:ethylene; D006861:Hydrogen Peroxide
keywords: Cadmium stress; Hormones; Hydrogen peroxide; Poplar; Proteomics