title: Quercetin protects against perfluorooctanoic acid-induced liver injury by attenuating oxidative stress and inflammatory response in mice.
abstract: The aim of the present study was to investigate the protective effect of quercetin (Que) against perfluorooctanoic acid (PFOA)-induced liver injury in mice and its possible mechanisms of action. Mice were intragastrically administered PFOA (10mg/kg/day) alone or in combination with Que (75 mg/kg/day) for 14 consecutive days. The hepatic injury was evaluated by measuring morphological changes, liver function, oxidative stress, inflammatory response and hepatocellular apoptosis. Compared with mice treated with PFOA alone, simultaneous supplementation of Que significantly decreased serum levels of liver injury indicators alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and total bile acids. Moreover, Que treatment inhibited the production of oxidative stress biomarkers malondialdehyde, hydrogen peroxide and 8-hydroxy-2'-deoxyguanosine, reduced the levels of proinflammatory cytokines interleukin 6, cyclooxygenase-2 and C-reactive protein, and decreased the number of TUNEL-positive cells in the liver of PFOA-treated mice. These results combined with liver histopathology demonstrated that Que exhibited a potential protective effect against PFOA-induced liver damage via mechanisms involving the attenuation of oxidative stress, alleviation of inflammation and inhibition of hepatocellular apoptosis.
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Zou
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zou
authors.affiliation: Library, Nanchang University, Nanchang 330006, PR China.
authors.forename: Fangming
authors.initials: F
authors.lastname: Liu
authors.affiliation: Library, Nanchang University, Nanchang 330006, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang 330006, PR China. Electronic address: zhangdalei@ncu.edu.cn.
authors.forename: Dalei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001647:Bile Acids and Salts / Q000378:metabolism; D002210:Caprylates; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D005466:Fluorocarbons; D022781:Hepatocytes / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000627:therapeutic use*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D001647:Bile Acids and Salts; D002210:Caprylates; D016207:Cytokines; D005466:Fluorocarbons; C023036:perfluorooctanoic acid; D011794:Quercetin
keywords: Apoptosis; Inflammation; Liver injury; Oxidative stress; Perfluorooctanoic acid; Quercetin