title: Amplified electrochemical hydrogen peroxide reduction based on hemin/G-quadruplex DNAzyme as electrocatalyst at gold particles modified heated copper disk electrode.
abstract: A new gold particles modified heated copper disk electrode (Au-HCuDE) with direct current was fabricated. The hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) was self-assembled on the heated electrode and resulted in a new biosensor denoted as HRP-DNAzyme/Au-HCuDE. By controlling the temperature of the surface of the electrode, the dramatic temperature effect on the electrocatalytic reduction of H2O2 at HRP-DNAzyme/Au-HCuDE sensing platform was demonstrated. This electrocatalytic activity of HRP-DNAzyme was enhanced with electrode temperature elevated. This method was thus preliminarily used to develop an electrochemical biosensor for highly sensitive detection of H2O2. A detection limit of 1.6×10(-7) M could be obtained (S/N=3) with an electrode temperature of 50 °C, which was more than one magnitude lower than that at electrode temperature of 0 °C. This heated electrochemical biosensor shows many merits such as easy fabrication and simple heating equipment, low cost, high thermal stability, and high sensitivity and good reproducibility.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: You
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Xian-Gui
authors.initials: XG
authors.lastname: Ma
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Song-Mao
authors.initials: SM
authors.lastname: Tian
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: jjsun@fzu.edu.cn.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Sun
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper; D021881:DNA, Catalytic; D055664:Electrochemical Techniques / Q000379:methods*; D054856:G-Quadruplexes; D006046:Gold; D006427:Hemin; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning
chemical_list: D021881:DNA, Catalytic; D006427:Hemin; D006046:Gold; D003300:Copper; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Electrocatalysis; Gold particles modified heated copper disk electrode; H(2)O(2); Hemin/G-quadruplex DNAzyme; Horseradish peroxidase-mimicking DNAzyme