title: Development of an effective and efficient DNA isolation method for Cinnamomum species.
abstract: Different species of Cinnamomum are rich in polysaccharide's and secondary metabolites, which hinder the process of DNA extraction. High quality DNA is the pre-requisite for any molecular biology study. In this paper we report a modified method for high quality and quantity of DNA extraction from both lyophilized and non-lyophilized leaf samples. Protocol reported differs from the CTAB procedure by addition of higher concentration of salt and activated charcoal to remove the polysaccharides and polyphenols. Wide utility of the modified protocol was proved by DNA extraction from different woody species and 4 Cinnamomum species. Therefore, this protocol has also been validated in different species of plants containing high levels of polyphenols and polysaccharides. The extracted DNA showed perfect amplification when subjected to RAPD, restriction digestion and amplification with DNA barcoding primers. The DNA extraction protocol is reproducible and can be applied for any plant molecular biology study.
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India. Electronic address: bsbhau@gmail.com.
authors.forename: B S
authors.initials: BS
authors.lastname: Bhau
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: G
authors.initials: G
authors.lastname: Gogoi
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: D
authors.initials: D
authors.lastname: Baruah
authors.affiliation: Biotechnology Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: R
authors.initials: R
authors.lastname: Ahmed
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: G
authors.initials: G
authors.lastname: Hazarika
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: B
authors.initials: B
authors.lastname: Borah
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: B
authors.initials: B
authors.lastname: Gogoi
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Sarmah
authors.affiliation: Plant Genomics Laboratory, Medicinal Aromatic & Economic Plants (MAEP) Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: S C
authors.initials: SC
authors.lastname: Nath
authors.affiliation: Biotechnology Division, CSIR-Northeast Institute of Science & Technology (CSIR-NEIST), Jorhat 785006, Assam, India.
authors.forename: S B
authors.initials: SB
authors.lastname: Wann
mesh_terms: D027424:Cinnamomum / Q000737:chemistry*; D017931:DNA Primers; D018744:DNA, Plant / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D059808:Polyphenols; D011134:Polysaccharides
keywords: Activated charcoal; CTAB; Cinnamomum; DNA; DNA isolation; PCR reaction; Polyvinyl pyrrolidone