title: Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria.
abstract: Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated. Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively. Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138MPa for 3cycles. The composite films made from the micro-emulsions significantly (p<0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4logCFU/ml in TSB after 2days at 22°C and on meat samples after 35days at 10°C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects. This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Ave., Wuxi, Jiangsu 214122, China; U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 190382, United States.
authors.forename: Mingming
authors.initials: M
authors.lastname: Guo
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 190382, United States. Electronic address: tony.jin@ars.usda.gov.
authors.forename: Tony Z
authors.initials: TZ
authors.lastname: Jin
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 190382, United States.
authors.forename: Madhav P
authors.initials: MP
authors.lastname: Yadav
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Ave., Wuxi, Jiangsu 214122, China. Electronic address: rjyang2009@hotmail.com.
authors.forename: Ruijin
authors.initials: R
authors.lastname: Yang
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology; D015169:Colony Count, Microbial; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology*; D005516:Food Microbiology / Q000379:methods*; D018857:Food Packaging / Q000592:standards*; D008087:Listeria / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D004655:Emulsions; D048271:Chitosan; D019342:Acetic Acid
keywords: Acetic acid (PubChem CID: 176); Allyl isothiocyanate; Allyl isothiocyanate (PubChem CID: 5971); Barley straw arabinoxylan; Chitosan; Chitosan (PubChem CID: 21896651); High pressure homogenization; Lactic acid (PubChem CID: 612); Levulinic acid (PubChem CID: 11579); Listeria; Micro-emulsion; Tween 80 (PubChem CID: 5281955)