title: The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components.
abstract: Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe the host cell-derived proteome of isolated C. trachomatis inclusions by quantitative proteomics. Computational analysis indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells' antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular, SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, these findings suggest that C. trachomatis hijacks retrograde pathways for effective infection.
authors.affiliation: Junior Research Group `Sexually Transmitted Bacterial Pathogens´, Robert Koch Institute, Berlin, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Aeberhard
authors.affiliation: Junior Research Group `Sexually Transmitted Bacterial Pathogens´, Robert Koch Institute, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Banhart
authors.affiliation: Junior Research Group `Bioinformatics´, Robert Koch Institute, Berlin, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Fischer
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: Nico
authors.initials: N
authors.lastname: Jehmlich
authors.affiliation: Junior Research Group `Sexually Transmitted Bacterial Pathogens´, Robert Koch Institute, Berlin, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Rose
authors.affiliation: Junior Research Group `Sexually Transmitted Bacterial Pathogens´, Robert Koch Institute, Berlin, Germany.
authors.forename: Sophia
authors.initials: S
authors.lastname: Koch
authors.affiliation: Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute, Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Laue
authors.affiliation: Junior Research Group `Bioinformatics´, Robert Koch Institute, Berlin, Germany.
authors.forename: Bernhard Y
authors.initials: BY
authors.lastname: Renard
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, Ernst-Moritz-Arndt-University, Greifswald, Germany; ZIK-FunGene Junior Research Group `Applied Proteomics´, Interfaculty Institute for Genetics and Functional Genomics, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schmidt
authors.affiliation: Junior Research Group `Sexually Transmitted Bacterial Pathogens´, Robert Koch Institute, Berlin, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Heuer
mesh_terms: D002469:Cell Separation; D002690:Chlamydia Infections / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000378:metabolism / Q000472:pathogenicity*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D021381:Protein Transport; D020543:Proteome; D040901:Proteomics; D034741:RNA, Small Interfering; D053719:Tandem Mass Spectrometry; D014162:Transfection; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D020543:Proteome; D034741:RNA, Small Interfering