title: Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation.
abstract: UV-B radiation is a potential stressor to the aquacultural species. Catla catla, catla larvae (1.08±0.065g) were exposed to different doses of UV-B radiation, 0 (control), 504, 1008, 1512 and 2016mJ/cm(2) at a mean radiant energy of 80μW/cm(2) for 21days. The dose of UV-B radiation was selected on the basis of the field study conducted in Lake Naini, Delhi, India (Latitude: 28°41'26″N and Longitude: 77°12″37″E). Significantly (P<0.05) lower survival, average weight and specific growth rate were found in UV-B irradiated larvae compared to the control one. Food conversion ratio was 1.5-4-fold higher in UV-B treated larvae compared to the control one. The carbonyl protein (CP), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD) levels were significantly (P <0.05) higher in UV-B irradiated larvae compared to the control group. Among the treated larvae, CP and SOD were significantly (P <0.05) higher in larvae exposed at 1512mJ/cm(2) UV-B. A correlation was found between the CP and SOD (R(2)=0.834). Highest TBARS level was found in 2016mJ/cm(2) UV-B exposed catla. Nitric oxide synthase level was significantly (P <0.05) lower in UV-B exposed larvae compared to the control one. A 3-fold increased Hsp 70 level was recorded in UV-B irradiated catla compared to the control larvae. Comet assay analysis indicated that UV-B irradiation enhanced DNA fragmentation. Tail extent moment and the olive tail moment were significantly (P <0.05) higher in 2016mJ/cm(2) UV-B exposed catla compared to others. The tail length was significantly (P <0.05) higher in 1512 and 2016mJ/cm(2) UV-B exposed larvae compared to the other doses. The present study suggests that the catla is a useful species for the biomonitoring of stress in the aquatic environment.
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110 007, India.
authors.forename: Moirangthem Kameshwor
authors.initials: MK
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Delhi Technological University, Bawana Road, Delhi 110042, India.
authors.forename: Jai Gopal
authors.initials: JG
authors.lastname: Sharma
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110 007, India. Electronic address: aquaresearchlab@yahoo.co.in.
authors.forename: Rina
authors.initials: R
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D053938:DNA Fragmentation / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects*; D016019:Survival Analysis; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D018840:HSP70 Heat-Shock Proteins
keywords: Carbonyl protein; Catla catla; DNA damage; Hsp 70; Superoxide dismutase; UV-B