title: The influence of bile acids homeostasis by cryptotanshinone-containing herbs.
abstract: BACKGROUND
  Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids.
  
  
  OBJECTIVE
  To investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids.
  
  
  METHODS
  The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS.
  
  
  RESULTS
  Initial screening showed that 100 µM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuronidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour.
  
  
  CONCLUSIONS
  All these data indicated the possibility of cryptotanshinone's influence towards bile acids metabolism and homeostasis of bile acids.
authors.affiliation: Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P. R. China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P. R. China.
authors.forename: Tianheng
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P. R. China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P. R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xie
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000037:antagonists & inhibitors; D006706:Homeostasis / Q000187:drug effects*; D006801:Humans; D010616:Phenanthrenes / Q000494:pharmacology*; D028321:Plant Preparations; D018517:Plant Roots; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D001647:Bile Acids and Salts; D010616:Phenanthrenes; D028321:Plant Preparations; C037886:cryptotanshinone; D014453:Glucuronosyltransferase
keywords: herbs; homeostasis; lithocholic acid (LCA)