title: Development of a novel biosensor based on F(0)F(1)-ATPase for the detection of 2-dodecylcyclobutanone in irradiated beef.
abstract: A novel biosensor regulated by the rotator of F0F1-ATPase was developed to analyze 2-dodecylcyclobutanone (2-DCB) to detect γ-ray irradiated beef rapidly. The biosensor was assembled by conjugating 2-DCB monoclonal antibodies with the "rotator" ε-subunit of F0F1-ATPase within chromatophores through an ε-subunit monoclonal antibody-biotin-avidin-biotin linker. The limit of detection (LOD) of 2-DCB was approximately 10(-8) μg/mL. The recovery ratio of 2-DCB from ground beef patties ranged from 75.1% to 116.4%. The intra-assay and inter-assay coefficients of variation were both <15.0%. The proposed method was validated by gas chromatography-mass spectrometry with high correlation. The biosensor was used to detect 2-DCB in ground beef patties with different fat contents (10%, 20%, and 30%) irradiated at 0.5, 1.0, 3.0, 5.0, and 7.0 kGy. The 2-DCB concentration linearly increased with the radiation dose in all the beef samples. 2-DCB concentration increased with fat levels in the three samples.
authors.affiliation: Institute of Agro-food Science and Technology, Chinese Academy of Agricultural Sciences/Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China.
authors.forename: Yueliang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Agro-food Science and Technology, Chinese Academy of Agricultural Sciences/Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Ha
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jiachang
authors.initials: J
authors.lastname: Yue
authors.affiliation: Institute of Agro-food Science and Technology, Chinese Academy of Agricultural Sciences/Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China. Electronic address: wangfengketizu@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003503:Cyclobutanes / Q000032:analysis*; D005514:Food Irradiation; D008401:Gas Chromatography-Mass Spectrometry; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D000069466:Red Meat / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D003503:Cyclobutanes; D006180:Proton-Translocating ATPases; C063381:2-dodecylcyclobutanone
keywords: 2-Dodecylcyclobutanone; F(0)F(1)-ATPase; GC–MS; Immuno-rotary biosensor; Irradiated beef