title: A preliminary X-ray study of 3-deoxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from Burkholderia pseudomallei.
abstract: 3-Deoxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI), the third enzyme in the pathway for the biosynthesis of 3-deoxy-D-manno-oct-2-ulosonic acid (KDO), hydrolyzes KDO 8-phosphate to KDO and inorganic phosphate. YrbI belongs to the haloacid dehalogenase (HAD) superfamily, which is a large family of magnesium-dependent phosphatase/phosphotransferase enzymes. In this study, YrbI from Burkholderia pseudomallei, the causative agent of melioidosis, has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.25 Å resolution. The crystal belonged to the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 63.7, b = 97.5, c = 98.0 Å. A full structural determination is in progress to elucidate the structure-function relationship of this protein.
authors.affiliation: Global Top 5 Research Program, College of Pharmacy, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Jimin
authors.initials: J
authors.lastname: Park
authors.affiliation: Global Top 5 Research Program, College of Pharmacy, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Daeun
authors.initials: D
authors.lastname: Lee
authors.affiliation: Global Top 5 Research Program, College of Pharmacy, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: 402-803 Technopark, 65 Pyeongchon-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-734, Republic of Korea.
authors.forename: Dae Yong
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Global Top 5 Research Program, College of Pharmacy, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Dong Hae
authors.initials: DH
authors.lastname: Shin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000201:enzymology; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D010744:Phosphoric Monoester Hydrolases; C024329:3-deoxy-manno-octulosonate-8-phosphatase
keywords: 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase; Burkholderia pseudomallei; YrbI