title: Genome wide transcriptional profiling of Herbaspirillum seropedicae SmR1 grown in the presence of naringenin.
abstract: Herbaspirillum seropedicae is a diazotrophic bacterium which associates endophytically with economically important gramineae. Flavonoids such as naringenin have been shown to have an effect on the interaction between H. seropedicae and its host plants. We used a high-throughput sequencing based method (RNA-Seq) to access the influence of naringenin on the whole transcriptome profile of H. seropedicae. Three hundred and four genes were downregulated and seventy seven were upregulated by naringenin. Data analysis revealed that genes related to bacterial flagella biosynthesis, chemotaxis and biosynthesis of peptidoglycan were repressed by naringenin. Moreover, genes involved in aromatic metabolism and multidrug transport efllux were actived.
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Michelle Z
authors.initials: MZ
authors.lastname: Tadra-Sfeir
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil ; Instituto Carlos Chagas, Fundação Oswaldo Cruz, Fiocruz-PR Curitiba, Brazil.
authors.forename: Helisson
authors.initials: H
authors.lastname: Faoro
authors.affiliation: Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina Londrina, Brazil.
authors.forename: Doumit
authors.initials: D
authors.lastname: Camilios-Neto
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Liziane
authors.initials: L
authors.lastname: Brusamarello-Santos
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Balsanelli
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Vinicius
authors.initials: V
authors.lastname: Weiss
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Valter A
authors.initials: VA
authors.lastname: Baura
authors.affiliation: Department of Genetics, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Roseli
authors.initials: R
authors.lastname: Wassem
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Cruz
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: De Oliveira Pedrosa
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Emanuel M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Nitrogen Fixation group, Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná Curitiba, Brazil.
authors.forename: Rose A
authors.initials: RA
authors.lastname: Monteiro
keywords: H. seropedicae; RNAseq; naringenin; plant-bacteria interaction; transcription regulation