title: Look-back study on recipients of Parvovirus B19 (B19V) DNA-positive blood components.
abstract: OBJECTIVE
  To assess the relevance of Parvovirus B19 (B19V) DNA at low to intermediate concentrations in blood donors for the recipients of their blood components.
  
  
  METHODS
  We studied recipients of B19V DNA-positive blood components [red blood cell concentrates (RBCs), pooled platelet concentrates and fresh frozen plasma]. This included archived pretransfusion samples as well as follow-up samples investigated by ELISA or NAT and genome sequence analysis.
  
  
  RESULTS
  In 132 out of 424 recipients, we could detect no anti-B19V IgG before transfusion. In 67 out of 132 sero-negative recipients, a follow-up sample was available. Sixty-five of these received blood components from donors with <10(4) IU B19V DNA/ml plasma and had no evidence of transfusion-transmitted (TT)-B19V infection. Homology in genome sequences in donor and recipient provided evidence for a TT-B19V infection in two recipients. Both patients received RBC containing 3.4 × 10(6) and 1.8 × 10(4) IU B19V DNA/ml plasma, respectively. The anti-B19V IgG titres in the donors were 2 and 76 IU/ml plasma, respectively. The antibodies in the second donor were directed against capsid proteins and are thus considered as potential neutralizing antibodies.
  
  
  CONCLUSIONS
  TT-B19V infections through blood components with low (<10(4) IU/ml plasma) B19V DNA concentrations did not occur in our study. One of the TT-B19V infections occurred from RBC with intermediate B19V DNA concentration despite the presence of potential neutralizing antibodies in the donor, but its clinical significance was low.
authors.affiliation: Institute of Transfusion Medicine, University Hospital of Schleswig-Holstein, Lübeck/Kiel, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Juhl
authors.affiliation: Institute of Transfusion Medicine, University Hospital of Schleswig-Holstein, Lübeck/Kiel, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Özdemir
authors.affiliation: Institute for Laboratory and Transfusion Medicine, Heart and Diabetes Center North Rhine-Westphalia, Bad Oeynhausen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Dreier
authors.affiliation: Institute of Transfusion Medicine, University Hospital of Schleswig-Holstein, Lübeck/Kiel, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Görg
authors.affiliation: Institute of Transfusion Medicine, University Hospital of Schleswig-Holstein, Lübeck/Kiel, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Hennig
mesh_terms: D000328:Adult; D001483:Base Sequence; D016913:Blood Component Transfusion; D001782:Blood Donors; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000097:blood* / Q000635:transmission; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral
keywords: B19V; Parvovirus B19 infection; blood donors; transfusion-transmitted infection