title: Increased Skeletal Muscle Capillarization Independently Enhances Insulin Sensitivity in Older Adults After Exercise Training and Detraining.
abstract: Intramuscular signaling and glucose transport mechanisms contribute to improvements in insulin sensitivity after aerobic exercise training. This study tested the hypothesis that increases in skeletal muscle capillary density (CD) also contribute to exercise-induced improvements in whole-body insulin sensitivity (insulin-stimulated glucose uptake per unit plasma insulin [M/I]) independent of other mechanisms. The study design included a 6-month aerobic exercise training period followed by a 2-week detraining period to eliminate short-term effects of exercise on intramuscular signaling and glucose transport. Before and after exercise training and detraining, 12 previously sedentary older (65 ± 3 years) men and women underwent research tests, including hyperinsulinemic-euglycemic clamps and vastus lateralis biopsies. Exercise training increased Vo2max (2.2 ± 0.2 vs. 2.5 ± 0.2 L/min), CD (313 ± 13 vs. 349 ± 18 capillaries/mm(2)), and M/I (0.041 ± 0.005 vs. 0.051 ± 0.007 μmol/kg fat-free mass/min) (P < 0.05 for all). Exercise training also increased the insulin activation of glycogen synthase by 60%, GLUT4 expression by 16%, and 5' AMPK-α1 expression by 21%, but these reverted to baseline levels after detraining. Conversely, CD and M/I remained 15% and 18% higher after detraining, respectively (P < 0.05), and the changes in M/I (detraining minus baseline) correlated directly with changes in CD in regression analysis (partial r = 0.70; P = 0.02). These results suggest that an increase in CD is one mechanism contributing to sustained improvements in glucose metabolism after aerobic exercise training.
authors.affiliation: Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD Baltimore Veterans Affairs Geriatric Research Education and Clinical Center and Research and Development Service, Baltimore, MD sprior@grecc.umaryland.edu.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Prior
authors.affiliation: Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD Baltimore Veterans Affairs Geriatric Research Education and Clinical Center and Research and Development Service, Baltimore, MD.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Goldberg
authors.affiliation: Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD Baltimore Veterans Affairs Geriatric Research Education and Clinical Center and Research and Development Service, Baltimore, MD.
authors.forename: Heidi K
authors.initials: HK
authors.lastname: Ortmeyer
authors.affiliation: Department of Kinesiology, University of Maryland School of Public Health, College Park, MD.
authors.forename: Eva R
authors.initials: ER
authors.lastname: Chin
authors.affiliation: Department of Kinesiology, University of Maryland School of Public Health, College Park, MD.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Chen
authors.affiliation: Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD Baltimore Veterans Affairs Geriatric Research Education and Clinical Center and Research and Development Service, Baltimore, MD.
authors.forename: Jacob B
authors.initials: JB
authors.lastname: Blumenthal
authors.affiliation: Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD Baltimore Veterans Affairs Geriatric Research Education and Clinical Center and Research and Development Service, Baltimore, MD.
authors.forename: Alice S
authors.initials: AS
authors.lastname: Ryan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002196:Capillaries / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism
chemical_list: D005947:Glucose