title: CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.
abstract: Tumour-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. The maturation and differentiation of tissue macrophages depends upon interactions between colony-stimulating factor 1 receptor (CSF1R) and its ligands. There remains, however, a lack of consistent information on CSF1R expression in TAMs. A new monoclonal antibody, FER216, was generated to investigate CSF1R protein distribution in formalin fixed tissue samples from 24 reactive lymphoid tissues and 187 different lymphoma types. We also analysed the distribution of CSF1R+, CD68+ and CD163+ macrophages by double immunostaining, and studied the relationship between CSF1R expression and survival in an independent series of 249 cHL patients. CSF1R+ TAMs were less frequent in B-cell lymphocytic leukaemia and lymphoblastic B-cell lymphoma than in diffuse large B-cell lymphoma, peripheral T-cell lymphoma, angioimmunoblastic T-cell lymphoma and cHL. HRS cells in cHL and, with the exception of three cases of anaplastic large cell lymphoma, the neoplastic cells in NHLs, lacked detectable CSF1R protein. A CSF1R+ enriched microenvironment in cHL was associated with shorter survival in an independent series of 249 cHL patients. CSF1R pathway activation was evident in the cHL and inactivation of this pathway could be a potential therapeutic target in cHL cases.
authors.affiliation: Pathology Department, MD Anderson Cancer Center, Madrid, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Martín-Moreno
authors.affiliation: Monoclonal Antibodies Unit, Spanish National Cancer Centre (CNIO), Madrid, Spain.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Roncador
authors.affiliation: Monoclonal Antibodies Unit, Spanish National Cancer Centre (CNIO), Madrid, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Maestre
authors.affiliation: Pathology Department, MD Anderson Cancer Center, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Mata
authors.affiliation: Monoclonal Antibodies Unit, Spanish National Cancer Centre (CNIO), Madrid, Spain.
authors.forename: Scherezade
authors.initials: S
authors.lastname: Jiménez
authors.affiliation: Proteomics Unit, Spanish National Cancer Centre (CNIO), Madrid, Spain.
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Martínez-Torrecuadrada
authors.affiliation: Monoclonal Antibodies Unit, Spanish National Cancer Centre (CNIO), Madrid, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Reyes-García
authors.affiliation: Pathology Department, MD Anderson Cancer Center, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rubio
authors.affiliation: Haematology Department, MD Anderson Cancer Center, Madrid, Spain.
authors.forename: José F
authors.initials: JF
authors.lastname: Tomás
authors.affiliation: Haematology Department, MD Anderson Cancer Center, Madrid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Estévez
authors.affiliation: Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
authors.forename: Karen
authors.initials: K
authors.lastname: Pulford
authors.affiliation: Pathology Department, Hospital Universitario Marqués de Valdecilla, Santander, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Piris
authors.affiliation: Pathology Department, MD Anderson Cancer Center, Madrid, Spain.
authors.forename: Juan F
authors.initials: JF
authors.lastname: García
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015870:Gene Expression; D006689:Hodgkin Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008221:Lymphoid Tissue / Q000378:metabolism* / Q000473:pathology; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D016186:Receptor, Macrophage Colony-Stimulating Factor