title: Characteristic Formation of Hyaluronan-Cartilage Link Protein-Proteoglycan Complex in Salivary Gland Tumors.
abstract: Hyaluronan (HA) and its binding molecules, cartilage link protein (LP) and proteoglycan (PG), are structural components of the hydrated extracellular matrix. Because these molecules play important roles in the tumor microenvironment, we examined the distribution of HA, LP, versican, and aggrecan in salivary gland tumors using histochemical and immunohistochemical methods, including double staining. LP was present in pleomorphic adenoma (PA) and adenoid cystic carcinoma (ACC) tissues, and aggrecan was absent in the malignant tumors that we investigated. LP colocalized with both HA and aggrecan in the chondromyxoid matrix of PA, suggesting the presence of a HA-LP-aggrecan complex. Furthermore, the HA-LP-versican complex could be observed in the pseudocystic space of the cribriform structures in ACC. The characteristic HA-LP-PG complex in PA and ACC might play a role in the behavior of tumors, and immunohistochemical analysis of these molecules could represent a diagnostic adjunct for salivary gland tumors.
authors.affiliation: Departments of *Pathology †Otorhinolaryngology, Osaka Medical College, Takatsuki ‡Department of Anatomy, Kansai University of Welfare Science, Kashiwara §Department of Advance Medicine, National Center for Geriatrics and Gerontology, Obu ∥Biochemistry and Molecular Biology Laboratory, Department of Nursing and Health, Aichi Prefectural University, Nagoya, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kuwabara
authors.forename: Akira
authors.initials: A
authors.lastname: Nishikado
authors.forename: Hana
authors.initials: H
authors.lastname: Hayasaki
authors.forename: Zenzo
authors.initials: Z
authors.lastname: Isogai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Yoneda
authors.forename: Ryo
authors.initials: R
authors.lastname: Kawata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hirose
mesh_terms: D002356:Cartilage / Q000378:metabolism*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D059016:Tumor Microenvironment
chemical_list: D011506:Proteins; D011509:Proteoglycans; D006820:Hyaluronic Acid