title: Grouper voltage-dependent anion selective channel protein 2 is required for nervous necrosis virus infection.
abstract: Nervous necrosis virus (NNV) is a non-enveloped virus with 2 segmented positive-sense single-stranded RNAs. NNV-induced mass mortality has occurred worldwide in many species of cultured marine fish and resulted in substantial economic losses. In our previous study, we cloned the gene of voltage-dependent anion selective channel protein 2 (GVDAC2), and the NNV RNA2 expression level decreased in GVDAC2-knockdown GF-1 cells 24 h after infection. Here, we investigated the role of GVDAC2 in the NNV infection in GF-1 cells. NNV infection did not considerably affect GVDAC2 gene expression. After performing immunostaining, we detected GVDAC2 at the mitochondria and GVDAC2 was colocalized with NNV-RNA-dependent RNA polymerase. However, these 2 proteins did not interact with each other in immunoprecipitation assay. The cellular ATP level in GVDAC2-downregulated cells was lower than that in control cells, and NNV-induced apoptosis was delayed in GVDAC2-siRNA-transfected cells. Therefore, we suggest that GVDAC2 is required for NNV infection for maintaining the cellular ATP level and has positive impact on virus-induced apoptosis.
authors.affiliation: Department of Life Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Jui-Shin
authors.initials: JS
authors.lastname: Chang
authors.affiliation: Department of Life Science, National Taiwan University, Taipei 10617, Taiwan. Electronic address: shauchi@ntu.edu.tw.
authors.forename: Shau-Chi
authors.initials: SC
authors.lastname: Chi
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001492:Bass; D015536:Down-Regulation; D005393:Fish Diseases / Q000235:genetics* / Q000821:virology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D030381:Nodaviridae / Q000502:physiology*; D012327:RNA Virus Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D050996:Voltage-Dependent Anion Channel 2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D034741:RNA, Small Interfering; D050996:Voltage-Dependent Anion Channel 2; D000255:Adenosine Triphosphate
keywords: Apoptosis; Grouper; Grouper voltage-dependent anion selective channel protein 2 (GVDAC2); Nervous necrosis virus (NNV)