title: The N-Linked Outer Chain Mannans and the Dfg5p and Dcw1p Endo-α-1,6-Mannanases Are Needed for Incorporation of Candida albicans Glycoproteins into the Cell Wall.
abstract: A biochemical pathway for the incorporation of cell wall protein into the cell wall of Neurospora crassa was recently proposed. In this pathway, the DFG-5 and DCW-1 endo-α-1,6-mannanases function to covalently cross-link cell wall protein-associated N-linked galactomannans, which are structurally related to the yeast outer chain mannans, into the cell wall glucan-chitin matrix. In this report, we demonstrate that the mannosyltransferase enzyme Och1p, which is needed for the synthesis of the N-linked outer chain mannan, is essential for the incorporation of cell wall glycoproteins into the Candida albicans cell wall. Using endoglycosidases, we show that C. albicans cell wall proteins are cross-linked into the cell wall via their N-linked outer chain mannans. We further demonstrate that the Dfg5p and Dcw1p α-1,6-mannanases are needed for the incorporation of cell wall glycoproteins into the C. albicans cell wall. Our results support the hypothesis that the Dfg5p and Dcw1p α-1,6-mannanases incorporate cell wall glycoproteins into the C. albicans cell wall by cross-linking outer chain mannans into the cell wall glucan-chitin matrix.
authors.affiliation: Department of Biological Sciences, SUNY University at Buffalo, Buffalo, New York, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Ao
authors.affiliation: School of Dental Medicine, Periodontics and Endodontics, SUNY University at Buffalo Dental School, Buffalo, New York, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Chinnici
authors.affiliation: School of Dental Medicine, Periodontics and Endodontics, SUNY University at Buffalo Dental School, Buffalo, New York, USA.
authors.forename: Abhiram
authors.initials: A
authors.lastname: Maddi
authors.affiliation: Department of Biological Sciences, SUNY University at Buffalo, Buffalo, New York, USA free@buffalo.edu.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Free
mesh_terms: D002176:Candida albicans / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005690:Galactose / Q000031:analogs & derivatives; D005936:Glucans / Q000378:metabolism; D008351:Mannans / Q000378:metabolism*; D008364:Mannosyltransferases / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D008351:Mannans; D008562:Membrane Glycoproteins; C012990:galactomannan; D002686:Chitin; D008364:Mannosyltransferases; D005690:Galactose