title: Regulation of three isoforms of SOD gene by environmental stresses in citrus red mite, Panonychus citri.
abstract: Superoxide dismutase (SOD) is a family of enzymes with multiple isoforms that possess antioxidative abilities in response to environmental stresses. Panonychus citri is one of the most important pest mites and has a global distribution. In this study, three distinct isoforms of SOD were cloned from P. citri and identified as cytoplasmic Cu-ZnSOD (PcSOD1), extracellular Cu-ZnSOD (PcSOD2), and mitochondrial MnSOD (PcSOD3). mRNA expression level analysis showed that all three isoforms were up-regulated significantly after exposure to the acaricide abamectin and to UV-B ultraviolet irradiation. In particular, PcSOD3 was up-regulated under almost all environmental stresses tested. The fold change of PcSOD3 expression was significantly higher than those of the two Cu-ZnSOD isoforms. Taken together, the results indicate that abamectin and UV-B can induce transcripts of all three SOD isoforms in P. citri. Furthermore, PcSOD3 seems to play a more important role in P. citri tolerance to oxidative stress.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Ying-Cai
authors.initials: YC
authors.lastname: Feng
authors.forename: Chong-Yu
authors.initials: CY
authors.lastname: Liao
authors.forename: Wen-Kai
authors.initials: WK
authors.lastname: Xia
authors.forename: Xuan-Zhao
authors.initials: XZ
authors.lastname: Jiang
authors.forename: Feng
authors.initials: F
authors.lastname: Shang
authors.forename: Guo-Rui
authors.initials: GR
authors.lastname: Yuan
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D040102:Tetranychidae / Q000235:genetics* / Q000378:metabolism
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D013482:Superoxide Dismutase