title: Analysis and measurement of dielectrophoretic manipulation of particles and lymphocytes using rail-type electrodes.
abstract: A particle manipulation and sorting device using the dielectrophoretic (DEP) force is described in this study. The device consists of "ladder-type", "flip-type" and "oblique rail-type" electrode regions. The ladder-type and rail-type electrodes can generate a DEP force distribution that captures the particles, the DEP force of which is negative, in the area located at the center of the electrodes. The ladder-type electrode can align the particles with equal spacing in the streamwise direction. Using the flip-type electrode, which pushes the particles away, in combination with these electrodes, the direction of the particle and timing can be selected with high accuracy, reliability, and response. In the first half of this study, a numerical simulation is carried out to calculate the particle motion and evaluate the performance of the ladder-type electrode. Several models are used to investigate the influences of the non-uniformity of the electric field and the electric interaction of the surface charges and polarizations. Experiments are then carried out to demonstrate the motions of the particles and the sorting reliability. The trajectories and the probability density functions of the particles at the inlet and outlet of the electrode region showed that by using these electrodes the particles can be aligned, sorted, and guided accurately.
authors.affiliation: Department of Mechanical Engineering and Science, Kyoto University, Japan. Electronic address: tatsumi@me.kyotou.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Tatsumi
authors.affiliation: Department of Mechanical Engineering and Science, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawano
authors.affiliation: Department of Mechanical Engineering and Science, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okui
authors.affiliation: Department of Mechanical Engineering and Science, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shintani
authors.affiliation: Department of Mechanical Engineering and Science, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakabe
mesh_terms: D017097:Electric Impedance; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation*; D008214:Lymphocytes / Q000166:cytology*; D008962:Models, Theoretical; D009038:Motion
keywords: Dielectrophoretic force; Lymphocytes; Manipulation; Microchannel flow; Microparticles; Sorting