title: Optimization of Purification, Identification and Evaluation of the in Vitro Antitumor Activity of Polyphenols from Pinus Koraiensis Pinecones.
abstract: In this study, an efficient purification method for the polyphenols of Pinus koraiensis pinecone (PPP) has been developed. AB-8 resin was verified to offer good adsorption and desorption ratio for PPP. Response surface methodology (RSM) indicated that the optimized purification parameters for PPP were 1.70 mg GAE/mL phenolic sample concentration, 22.00 mL sample volume, and 63.00% ethanol concentration. Under these conditions, the experimental purity of PPP was 27.93 ± 0.14% (n = 3), which matched well with the predicted purity of 28.17%. Next, the antiproliferative effects of PPP on seven cancer cell lines, including A375 (human skin melanoma cancer cell line), A549 (human lung cancer cell line), SH-SY5Y (human neuroblastoma cell line), LOVO (human colon cancer stem cell line), MCF-7 (human breast cancer cell line), HeLa (human cervical cancer line), and HT29 (human colon cancer line), were examined by MTT assays. The results indicated that PPP had the highest capacity for inhibiting LOVO cells growth with an EC50 value of 0.317 ± 0.0476 mg/mL. Finally, Ultra-high performance liquid chromatography- tandem mass spectrometry (UPLC-MS) was used to tentatively identify twenty-four peaks in the purified PPP, of which five representative peaks were identified as catechin, methyl quercetin, o-vanillin, luteolin and coronaric acid. Our results demonstrate that Pinus koraiensis pinecone is a readily available source of polyphenols, and the purified PPP could be a promising natural antitumor agent for applications in functional foods.
authors.affiliation: Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Yi
authors.affiliation: Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China. wangzhenyu13001@163.com.|Northeast Forestry University, 26 Hexing Road, Xiangfang District, Harbin 150040, China. wangzhenyu13001@163.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China.
authors.forename: Haina
authors.initials: H
authors.lastname: Bai
authors.affiliation: Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jing
authors.affiliation: Jilin Medical College, 5 Jilin street, Jilin 132013, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zuo
mesh_terms: D000327:Adsorption; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D010636:Phenols; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology*; D012116:Resins, Plant
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D012116:Resins, Plant
keywords: antiproliferative activity; compound identification; pinecone of Pinus koraiensis; polyphenols purification; response surface methodology