title: MiRNA-1469 promotes lung cancer cells apoptosis through targeting STAT5a.
abstract: MicroRNAs play key roles in cell growth, differentiation, and apoptosis. In this study, we described the regulation and function of miR-1469 in apoptosis of lung cancer cells (A549 and NCI-H1650). Expression analysis verified that miR-1469 expression significantly increased in apoptotic cells. Overexpression of miR-1469 in lung cancer cells increased cell apoptosis induced by etoposide. Additionally, we identified that Stat5a is a downstream target of miR-1469, which can bind directly to the 3'-untranslated region of the Stat5a, subsequently reducing both the mRNA and protein levels of Stat5a. Finally, co-expression of miR-1469 and Stat5a in A549 and NCI-H1650 cells partially abrogated the effect of miR-1469 on cell apoptosis. Our results show that miR-1469 functions as an apoptosis enhancer to regulate lung cancer apoptosis through targeting Stat5a and may become a critical therapeutic target in lung cancer.
authors.affiliation: Aviation Medicine Research Laboratory, Air Force General Hospital, PLA Beijing 100142, China.
authors.forename: Chengshan
authors.initials: C
authors.lastname: Xu
authors.affiliation: Aviation Medicine Research Laboratory, Air Force General Hospital, PLA Beijing 100142, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Aviation Medicine Research Laboratory, Air Force General Hospital, PLA Beijing 100142, China.
authors.forename: Hengheng
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, 100021, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Aviation Medicine Research Laboratory, Air Force General Hospital, PLA Beijing 100142, China.
authors.forename: Lianning
authors.initials: L
authors.lastname: Duan
authors.affiliation: Aviation Medicine Research Laboratory, Air Force General Hospital, PLA Beijing 100142, China.
authors.forename: Chengrong
authors.initials: C
authors.lastname: Lu
keywords: MicroRNA; Stat5a; apoptosis; lung cancer