title: Significance of CK19, TPO, and HBME-1 expression for diagnosis of papillary thyroid carcinoma.
abstract: OBJECTIVE
  To evaluate the expression and significance of CK19, TPO, and HBME-1 in the differential diagnosis of papillary thyroid carcinoma (PTC) and nonmalignant nodules.
  
  
  METHODS
  Tissue samples were obtained from 257 patients with PTC and 149 patients with nonmalignant thyroid specimens, and immunohistochemical staining for CK-19, TPO, and HBME-1 was performed.
  
  
  RESULTS
  The expression of CK-19, TPO, and HBME-1 was 96.3%, 12.0%, and 85.3%, respectively, for the PTC group. For nonmalignant thyroid lesions group, the expression of these markers was 40.4%, 86.2%, and 37.2%, respectively. Further, the expression of CK-19 and HBME-1 in PTCs was much higher than that in the benign thyroid lesions (P < 0.05). However, the positive expression of TPO in PTC specimens was much lower than that in the nonmalignant specimens (P < 0.05). CK-1 had the highest sensitivity (96.30%) for PTCs. The combination of the positive expression of CK-19 and negative expression of TPO had the highest sensitivity (98.50%), while that of the positive expression of HBME-1 and negative expression of TPO had the highest specificity (92.90%).
  
  
  CONCLUSIONS
  The combination of positive expression of CK-19 or HBME-1 or negative expression of TPO can improve the specificity of the diagnosis of PTC.
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Zeming
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, Hubei, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Yusufu
authors.initials: Y
authors.lastname: Maiaiti
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Shuntao
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ming
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
authors.affiliation: Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ge
authors.affiliation: Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science And Technology Wuhan, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
keywords: Papillary thyroid carcinoma; cytokeratin-19; human bone marrow endothelial cell-1; thyroid peroxidase