title: Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition.
abstract: Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecularly imprinted polymer microarrays on bare gold SPR imaging chips. During electrospotting both the gold chip and the spotting tip are electrically connected to a potentiostat as working and counter electrodes, respectively. The spotting pin encloses the monomer-template protein cocktail that upon contacting the gold surface is in-situ electropolymerized resulting in surface confined polymer spots of ca. 500 µm diameter. By repeating this procedure at preprogrammed locations for various composition monomer-template mixtures microarrays of nanometer-thin surface-imprinted films are generated in a controlled manner. We show that the removal and rebinding kinetics of the template and various potential interferents to such microarrays can be monitored in real-time and multiplexed manner by SPR imaging. The proof of principle for microelectrospotting of electrically insulating surface-imprinted films is made by using scopoletin as monomer and ferritin as protein template. It is shown that microelectrospotting in combination with SPR imaging can offer a versatile platform for label-free and enhanced throughput optimization of the molecularly imprinted polymers for protein recognition and for their analytical application.
authors.affiliation: Fraunhofer Institute IZI-BB, D-14476 Potsdam, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Bosserdt
authors.affiliation: MTA-BME Research Group of Technical Analytical Chemistry, Szt. Gellért tér 4, H-1111 Budapest, Hungary.
authors.forename: Júlia
authors.initials: J
authors.lastname: Erdőssy
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, H-1111 Budapest, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Lautner
authors.affiliation: Carl von Ossietzky University of Oldenburg, Department of Chemistry, D-26111 Oldenburg, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Witt
authors.affiliation: Fraunhofer Institute IZI-BB, D-14476 Potsdam, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Köhler
authors.affiliation: Fraunhofer Institute IZI-BB, D-14476 Potsdam, Germany.
authors.forename: Nenad
authors.initials: N
authors.lastname: Gajovic-Eichelmann
authors.affiliation: Fraunhofer Institute IZI-BB, D-14476 Potsdam, Germany.
authors.forename: Aysu
authors.initials: A
authors.lastname: Yarman
authors.affiliation: Carl von Ossietzky University of Oldenburg, Department of Chemistry, D-26111 Oldenburg, Germany.
authors.forename: Gunther
authors.initials: G
authors.lastname: Wittstock
authors.affiliation: Fraunhofer Institute IZI-BB, D-14476 Potsdam, Germany.
authors.forename: Frieder W
authors.initials: FW
authors.lastname: Scheller
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, H-1111 Budapest, Hungary; MTA-BME "Lendület" Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, H-1111 Budapest, Hungary. Electronic address: robertgy@mail.bme.hu.
authors.forename: Róbert E
authors.initials: RE
authors.lastname: Gyurcsányi
mesh_terms: D000818:Animals; D002417:Cattle; D055664:Electrochemical Techniques; D005293:Ferritins / Q000737:chemistry; D006046:Gold; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012603:Scopoletin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation
chemical_list: D011108:Polymers; D012710:Serum Albumin, Bovine; D006046:Gold; D005293:Ferritins; D012603:Scopoletin
keywords: Electropolymerization; Electrospotting; Ferritin; Molecularly imprinted polymers; Scopoletin; Surface imprinting