title: Lactoferrin and ovotransferrin contribute toward antioxidative effects of Edible Bird's Nest against hydrogen peroxide-induced oxidative stress in human SH-SY5Y cells.
abstract: There are reports of improved redox outcomes due to consumption of Edible Bird's Nest (EBN). Many of the functional effects of EBN can be linked to its high amounts of antioxidants. Interestingly, dietary components with high antioxidants have shown promise in the prevention of aging and its related diseases like Alzheimer's disease. In this study, the antioxidative potentials of EBN and its constituents, lactoferrin (LF) and ovotransferrin (OVF), were determined and protective effects against hydrogen peroxide (H2O2)- induced toxicity on SH-SY5Y cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and acridine orange and propidium iodide (AO/PI) staining with microscopy were examined. Results showed that EBN and its constituents attenuated H2O2-induced cytotoxicity, and decreased radical oxygen species (ROS) through increased scavenging activity. Furthermore, LF, OVF, and EBN produced transcriptional changes in antioxidant related genes that tended towards neuroprotection as compared to H2O2-treated group. Overall, the results suggest that LF and OVF may produce synergistic or all-or-none antioxidative effects in EBN.
authors.affiliation: a Laboratory of Molecular Biomedicine , Institute of Bioscience, Universiti Putra Malaysia , Serdang , Malaysia.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Hou
authors.forename: Mustapha Umar
authors.initials: MU
authors.lastname: Imam
authors.forename: Maznah
authors.initials: M
authors.lastname: Ismail
authors.forename: Nur Hanisah
authors.initials: NH
authors.lastname: Azmi
authors.forename: Norsharina
authors.initials: N
authors.lastname: Ismail
authors.forename: Aini
authors.initials: A
authors.lastname: Ideris
authors.forename: Rozi
authors.initials: R
authors.lastname: Mahmud
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry; D001717:Birds; D045744:Cell Line, Tumor; D003207:Conalbumin / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007781:Lactoferrin / Q000302:isolation & purification / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D001688:Biological Products; D003207:Conalbumin; D006861:Hydrogen Peroxide; D007781:Lactoferrin; D018696:Neuroprotective Agents; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2; C000606290:SOD1 protein, human; C499320:PARP1 protein, human
keywords: Edible Bird’s Nest; antioxidant; lactoferrin; neuroprotection; ovotransferrin