title: Effect of Dietary Vitamin E Supplementation on Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress.
abstract: We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU α-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver α-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q9 (CoQ9), reduced CoQ9, reduced CoQ10, and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities increased more greatly in ND-fed rats than in VESD-fed rats but the serum stress marker levels increased to a similar extent. The WIRS exposure caused no change in liver LPO concentration with the further increase in liver α-tocopherol concentration in VESD-fed rats but increased liver LPO concentration without changing liver α-tocopherol concentration in ND-fed rats. Upon the WIRS exposure, liver reduced glutathione concentration decreased with the further decrease in liver ascorbic acid concentration in VESD-fed rats and those concentrations decreased in ND-fed rats. The WIRS exposure recovered the decreased liver total CoQ9 and reduced CoQ9 concentrations in VESD-fed rats but decreased liver total CoQ9, reduced CoQ9, and reduced CoQ10 concentrations in ND-fed rats. These results indicate that dietary vitamin E supplementation protects against liver oxidative damage without affecting the stress response in rats with WIRS.
authors.affiliation: Department of Chemistry, Fujita Health University School of Medicine.
authors.forename: Yoshiji
authors.initials: Y
authors.lastname: Ohta
authors.forename: Koji
authors.initials: K
authors.lastname: Yashiro
authors.forename: Koji
authors.initials: K
authors.lastname: Ohashi
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Horikoshi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kusumoto
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Matsura
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuzawa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D005978:Glutathione / Q000378:metabolism; D007101:Immersion; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000187:drug effects*; D017392:Thiobarbituric Acid Reactive Substances; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D014451:Ubiquinone; D014810:Vitamin E; C024989:coenzyme Q10; D005978:Glutathione; D024502:alpha-Tocopherol; C030571:ubiquinone 9; D001205:Ascorbic Acid