title: Higher transcription levels in ascorbic acid biosynthetic and recycling genes were associated with higher ascorbic acid accumulation in blueberry.
abstract: In our preliminary study, the ripe fruits of two highbush blueberry (Vaccinium corymbosum L.) cultivars, cv 'Berkeley' and cv 'Bluecrop', were found to contain different levels of ascorbic acid. However, factors responsible for these differences are still unknown. In the present study, ascorbic acid content in fruits was compared with expression profiles of ascorbic acid biosynthetic and recycling genes between 'Bluecrop' and 'Berkeley' cultivars. The results indicated that the l-galactose pathway was the predominant route of ascorbic acid biosynthesis in blueberry fruits. Moreover, higher expression levels of the ascorbic acid biosynthetic genes GME, GGP, and GLDH, as well as the recycling genes MDHAR and DHAR, were associated with higher ascorbic acid content in 'Bluecrop' compared with 'Berkeley', which indicated that a higher efficiency ascorbic acid biosynthesis and regeneration was likely to be responsible for the higher ascorbic acid accumulation in 'Bluecrop'.
authors.affiliation: College of Life Sciences, Ludong University, Yantai, Shandong 264025, PR China.
authors.forename: Fenghong
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Ludong University, Yantai, Shandong 264025, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Pomology, Yantai Academy of Agricultural Sciences, Yantai, Shandong 264025, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gu
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: suhongyan66@126.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Su
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: chengxianhao@sohu.com.
authors.forename: Xianhao
authors.initials: X
authors.lastname: Cheng
mesh_terms: D001205:Ascorbic Acid / Q000096:biosynthesis*; D029798:Blueberry Plants / Q000737:chemistry* / Q000235:genetics*; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000737:chemistry* / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism
chemical_list: D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C093910:galactonolactone dehydrogenase; C020666:glutathione dehydrogenase (ascorbate); D002238:Carbohydrate Epimerases; C019192:GDPmannose 3,5-epimerase; D001205:Ascorbic Acid
keywords: Blueberry; Fruit ripening; Gene expression; l-Ascorbic acid