title: Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by laccase from Trametes versicolor covalently immobilized on amino-functionalized SBA-15.
abstract: A covalent immobilization method based on glutaraldehyde and amino-functionalized SBA-15 supports has been successfully applied to covalently and stably immobilize laccase from Trametes versicolor. The resultant biocatalysts displayed high incorporation yields of enzyme and led to excellent biodegradation rates of selected HPAs models, i.e. naphthalene, phenanthrene and anthracene, in water. The nature of the hydrocarbon chain accompanying the amino group has been shown as determinant for the immobilization as well as for the activity and reusability of the materials. Thus, alkyl moieties displayed higher enzyme loadings than phenyl moieties, being more adequate the larger n-butyl tethering residue likely due to its higher mobility. Using the aminobutyl-based laccase-SBA-15, 82%, 73%, and 55% conversion of naphthalene, phenanthrene and anthracene, respectively, were achieved after 48 h, very close to the values obtained with free laccase under the same reaction conditions. On the other hand, aminopropyl-based laccase-SBA-15 biocatalysts displayed the best reusability properties, retaining higher activity after four repeated uses than the corresponding aminobutyl-based materials.
authors.affiliation: Department of Chemical and Energy Technology, Chemical and Environmental Technology, Mechanical Technology and Analytical Chemistry, ESCET, Universidad Rey Juan Carlos, C/Tulipán s/n, 28933 Móstoles, Madrid, Spain. Electronic address: fernando.bautista@urjc.es.
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Bautista
authors.affiliation: Department of Chemical and Energy Technology, Chemical and Environmental Technology, Mechanical Technology and Analytical Chemistry, ESCET, Universidad Rey Juan Carlos, C/Tulipán s/n, 28933 Móstoles, Madrid, Spain.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Morales
authors.affiliation: Department of Chemical and Energy Technology, Chemical and Environmental Technology, Mechanical Technology and Analytical Chemistry, ESCET, Universidad Rey Juan Carlos, C/Tulipán s/n, 28933 Móstoles, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Sanz
mesh_terms: D000363:Agaricales / Q000378:metabolism; D000873:Anthracenes; D001673:Biodegradation, Environmental; D042845:Laccase / Q000378:metabolism; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012822:Silicon Dioxide / Q000378:metabolism*; D055454:Trametes / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; C509969:SBA-15; C031721:naphthalene; C031181:phenanthrene; D012822:Silicon Dioxide; D042845:Laccase; C034020:anthracene
keywords: Biodegradation; Enzyme immobilization; Laccase; Polycyclic aromatic hydrocarbons; SBA-15