title: G protein-coupled odorant receptors: From sequence to structure.
abstract: Odorant receptors (ORs) are the largest subfamily within class A G protein-coupled receptors (GPCRs). No experimental structural data of any OR is available to date and atomic-level insights are likely to be obtained by means of molecular modeling. In this article, we critically align sequences of ORs with those GPCRs for which a structure is available. Here, an alignment consistent with available site-directed mutagenesis data on various ORs is proposed. Using this alignment, the choice of the template is deemed rather minor for identifying residues that constitute the wall of the binding cavity or those involved in G protein recognition.
authors.affiliation: Institute of Chemistry-Nice, UMR 7272 CNRS-University Nice-Sophia Antipolis, Nice Cedex 2, 06108, France.
authors.forename: Claire A
authors.initials: CA
authors.lastname: de March
authors.affiliation: Materials and Process Simulation Center (MC139-74), California Institute of Technology, Pasadena, California, 91125.
authors.forename: Soo-Kyung
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Institute of Chemistry-Nice, UMR 7272 CNRS-University Nice-Sophia Antipolis, Nice Cedex 2, 06108, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Antonczak
authors.affiliation: Materials and Process Simulation Center (MC139-74), California Institute of Technology, Pasadena, California, 91125.
authors.forename: William A
authors.initials: WA
authors.lastname: Goddard
authors.affiliation: Institute of Chemistry-Nice, UMR 7272 CNRS-University Nice-Sophia Antipolis, Nice Cedex 2, 06108, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Golebiowski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017124:Conserved Sequence; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D018035:Receptors, Odorant / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000379:methods; D013329:Structure-Activity Relationship
chemical_list: D043562:Receptors, G-Protein-Coupled; D018035:Receptors, Odorant
keywords: binding cavity; odorant receptor; olfactory; sequence; structure