title: Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice.
abstract: The aim of this study is to evaluate the protective effect and underlying mechanism of hydrogen gas (H2) to glyoxylate induced renal calcium oxalate (CaOx) crystal deposition in mice. In present work, rodent renal CaOx crystal deposition model was introduced by intra-abdominal injection of glyoxylate (100 mg/kg/d) for 5 days. Two days before administration of glyoxylate, inhalation of H2 for 30 min per day was initiated and continued for 7 days. By the end of the study, the samples of 24 hours urine, serum and renal tissue were collected for biochemical and pathological assay. According to levels of urine calcium excretion, renal calcium deposition, a serum excretion of kidney injury molecule-1 (KIM-1) assay and a TUNEL assay, inhalation of H2 could successfully decrease the CaOx crystallizations and protect against renal injury. Crystal deposition in the kidneys is associated with oxidative stress, which was indicated by increased levels of renal malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) and decreased activities of superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT). These effects were reversed by a high-dose H2 pretreatment. The renal expressions of osteopontin (OPN), CD44, monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10) were markedly increased in glyoxylate-treated mice, and H2 significantly attenuated the increase of OPN, CD44 and MCP-1 but upregulated the expression of IL-10. Our findings demonstrate that inhalation of H2 reduces renal crystallization, renal oxidative injury and inflammation and it may be a candidate agent with few adverse effects for prevention of nephrolithiasis.
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Zhongjiang
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University Shanghai, China.
authors.forename: Zhouheng
authors.initials: Z
authors.lastname: Ye
authors.affiliation: School of Pharmacy, Second Military Medical University Shanghai, China.
authors.forename: Songyan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University Shanghai, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004195:Disease Models, Animal; D006038:Glyoxylates / Q000633:toxicity; D006859:Hydrogen / Q000008:administration & dosage*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053040:Nephrolithiasis / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D006038:Glyoxylates; D006859:Hydrogen; C031150:glyoxylic acid
keywords: Hydrogen gas; calcium oxalate; glyoxylate; kidney injury molecule-1; oxidative stress