title: Resveratrol Decreases TXNIP mRNA and Protein Nuclear Expressions With an Arterial Function Improvement in Old Mice.
abstract: Aging leads to a high prevalence of glucose intolerance and cardiovascular diseases, with oxidative stress playing a potential role. Resveratrol has shown promising effects on glucose tolerance and tends to improve endothelial function in elderly patients. Thioredoxin-interacting protein (TXNIP) was recently proposed as a potential link connecting glucose metabolism to oxidative stress. Here, we investigated the resveratrol-induced improvement of arterial aging phenotype in old mice and the expression of aortic TXNIP. Using an in vivo model of old mice with or without 3-month resveratrol treatment, we investigated the effects of resveratrol on age-related impairments from a cardiovascular Doppler analysis, to a molecular level, by studying inflammation and oxidative stress factors. We found a dual effect of resveratrol, with a decrease of age-related glucose intolerance and oxidative stress imbalance leading to reduced matrix remodeling that forestalls arterial aging phenotype in terms of intima-media thickness and arterial distensibility. These results provide the first evidence that aortic TXNIP mRNA and protein nuclear expressions are increased in the arterial aging and decreased by resveratrol treatment. In conclusion, we demonstrated that resveratrol helped to restore several aging impaired processes in old mice, with a decrease of aortic TXNIP mRNA and protein nuclear expressions.
authors.affiliation: Faculty of Pharmacy, Inserm UMRS_1140, Paris, France. Paris Descartes University, Sorbonne Paris Cité, Paris, France. tbedarida@gsu.edu.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Bedarida
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Paris, France. Department of Physiology, Georges Pompidou European Hospital, AP-HP, Paris, France.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Baron
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Paris, France. Faculty of Pharmacy, UMR-S 1139, Paris, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Vibert
authors.affiliation: CNRS UMR 6214, INSERM U1083, Angers University, Angers, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Ayer
authors.affiliation: CNRS UMR 6214, INSERM U1083, Angers University, Angers, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Henrion
authors.affiliation: Biochemistry Laboratory, Trousseau Hospital, AP-HP, Paris, France.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Thioulouse
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Paris, France. PIPA, Cochin Institute - U1016, Paris, France.
authors.forename: Carmen
authors.initials: C
authors.lastname: Marchiol
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Paris, France. Faculty of Pharmacy, UMR-S 1139, Paris, France. Clinical Biochemistry, Necker Hospital, AP-HP, Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Beaudeux
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Paris, France. Clinical Biochemistry, Necker Hospital, AP-HP, Paris, France.
authors.forename: Charles-Henry
authors.initials: CH
authors.lastname: Cottart
authors.affiliation: Faculty of Pharmacy, Inserm UMRS_1140, Paris, France. Paris Descartes University, Sorbonne Paris Cité, Paris, France. Department of Biochemistry, Georges Pompidou European Hospital, AP-HP, Paris, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Nivet-Antoine
mesh_terms: D000818:Animals; D001011:Aorta / Q000000981:diagnostic imaging* / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D002352:Carrier Proteins / Q000378:metabolism*; D015150:Echocardiography, Doppler; D018149:Glucose Intolerance / Q000188:drug therapy*; D005951:Glucose Tolerance Test; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism*; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D013267:Stilbenes; C445672:Txnip protein, mouse; D013879:Thioredoxins; D000077185:Resveratrol; D002118:Calcium
keywords: Arterial aging; Glucose intolerance; Oxidative stress; Resveratrol; Thioredoxin-interacting protein