title: Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone.
abstract: A fast, simple, and sensitive flow injection analysis method was developed for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human serum. Benzaldehyde, generated by the action of SSAO after incubation of serum with benzylamine, was derivatized with a novel aromatic aldehyde-specific reagent (1,2-diaminoanthraquinone) and the fluorescent product was measured by fluorescence detection at excitation and emission wavelengths of 390 and 570nm, respectively. Serum SSAO activity was defined as benzaldehyde (nmol) formed per milliliter serum per hour. The method was linear over SSAO activity of 0.2-150.0nmolmL(-1)h(-1) with a detection limit of 0.06nmolmL(-1)h(-1). The %RSD of intra-day and inter-day precision did not exceed 9.4% and the accuracy ranged from -6.5 to -0.6%. The method was applied for the determination of the serum SSAO activity in healthy controls (C, n=24) and diabetes mellitus patients (DM, n=18). It was demonstrated that the activity (mean±SE) of SSAO in diabetics sera was significantly higher than that in healthy subjects' ones (DM; 73.3±1.8nmolmL(-1)h(-1)vs C; 58.9±2.2nmolmL(-1)h(-1), P<0.01).
authors.affiliation: Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan; Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Mahmoud H
authors.initials: MH
authors.lastname: El-Maghrabey
authors.affiliation: Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.affiliation: Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.affiliation: Sasebo Chuo Hospital, 15 Yamato-machi, Sasebo 857-1165, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Imazato
authors.affiliation: Sasebo Chuo Hospital, 15 Yamato-machi, Sasebo 857-1165, Japan.
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ueki
authors.affiliation: Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. Electronic address: n-kuro@nagasaki-u.ac.jp.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000880:Anthraquinones / Q000737:chemistry*; D001547:Benzaldehydes / Q000097:blood* / Q000737:chemistry; D001596:Benzylamines / Q000737:chemistry; D003920:Diabetes Mellitus / Q000097:blood / Q000201:enzymology; D005260:Female; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000032:analysis / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D000880:Anthraquinones; D001547:Benzaldehydes; D001596:Benzylamines; D007093:Imidazoles; C030796:benzylamine; D006631:Amine Oxidase (Copper-Containing); C118201:1,2-diaminoanthraquinone; C032175:benzaldehyde
keywords: 1,2-Diaminoanthraquinone; Benzaldehyde; Flow injection analysis; Fluorescence detection; Semicarbazide-sensitive amine oxidase activity