title: Evaluation of cationic micropeptides derived from the innate immune system as inhibitors of marine biofouling.
abstract: A series of 13 short synthetic amphiphilic cationic micropeptides, derived from the antimicrobial iron-binding innate defence protein lactoferrin, have been evaluated for their capacity to inhibit the marine fouling process. The whole biofouling process was studied and microfouling organisms such as marine bacteria and microalgae were included as well as the macrofouling barnacle Balanus improvisus. In total 19 different marine fouling organisms (18 microfoulers and one macrofouler) were included and both the adhesion and growth of the microfoulers were investigated. It was shown that the majority of the peptides inhibited barnacle cyprid settlement via a reversible nontoxic mechanism, with IC50 values as low as 0.5 μg ml(-1). Six peptides inhibited adhesion and growth of microorganisms. Two of these were particularly active against the microfoulers with MIC-values ranging between 0.01 and 1 μg ml(-1), which is comparable with the commercial reference antifoulant SeaNine.
authors.affiliation: a School of Biological Sciences , University of Portsmouth , Portsmouth , UK.
authors.forename: Rozenn
authors.initials: R
authors.lastname: Trepos
authors.forename: Gunnar
authors.initials: G
authors.lastname: Cervin
authors.forename: Claire
authors.initials: C
authors.lastname: Pile
authors.forename: Henrik
authors.initials: H
authors.lastname: Pavia
authors.forename: Claire
authors.initials: C
authors.lastname: Hellio
authors.forename: Johan
authors.initials: J
authors.lastname: Svenson
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000502:physiology*; D001419:Bacteria / Q000187:drug effects*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control*; D007113:Immunity, Innate; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D058086:Microalgae / Q000187:drug effects* / Q000502:physiology; D019151:Peptide Library; D001468:Thoracica / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D019151:Peptide Library; D007781:Lactoferrin
keywords: amphiphilic; antifouling; innate defence; nontoxic; peptides