title: Isolation and purification of phenylethanoid glycosides from Cistanche deserticola by high-speed counter-current chromatography.
abstract: Five phenylethanoid glycosides (PhGs), echinacoside, cistanoside A, acteoside, isoacteoside and 2'-acetylacteoside, were isolated and purified from Cistanche deserticola for the first time by high-speed counter-current chromatography (HSCCC) using two biphasic systems, one consisting of ethyl acetate-ethanol-water (5:0.5:4.5, v/v/v) and another of ethyl acetate-n-butanol-ethanol-water (0.5:0.5:0.1:1, v/v/v/v). A total of 28.5mg of echinacoside, 18.4mg of cistanoside A, 14.6mg of acteoside, 30.1mg of isoacteoside and 25.2mg of 2'-acetylacteoside were purified from 1412mg of the n-butanol extract of C. deserticola, each at over 92.5% purity as determined by HPLC. The structures were identified by their retention time, UV, LC-ESI-MS in the negative ion mode, and confirmed by NMR experiments. The characteristic LC-ESI-MS(n) fragmentation pattern of the five compounds is discussed, and found to be a very specific and useful tool for the structural identification of PhGs from this important medicinal plant.
authors.affiliation: Department of Chemistry, Changchun Normal University, Changchun 130032, China; Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9. Electronic address: caor@agr.gc.ca.
authors.forename: Rong
authors.initials: R
authors.lastname: Tsao
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9.
authors.forename: Raymond
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Changchun Normal University, Changchun 130032, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9.
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Young
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9.
authors.forename: Honghui
authors.initials: H
authors.lastname: Zhu
keywords: 2′-Acetylacteoside; Acteoside; Cistanche deserticola; Cistanoside A; Echinacoside; HSCCC; Isoacteoside; LC–ESI-MS; NMR; Phenylethanoid