title: Nup153 Recruits the Nup107-160 Complex to the Inner Nuclear Membrane for Interphasic Nuclear Pore Complex Assembly.
abstract: In metazoa, nuclear pore complexes (NPCs) are assembled from constituent nucleoporins by two distinct mechanisms: in the re-forming nuclear envelope at the end of mitosis and into the intact nuclear envelope during interphase. Here, we show that the nucleoporin Nup153 is required for NPC assembly during interphase but not during mitotic exit. It functions in interphasic NPC formation by binding directly to the inner nuclear membrane via an N-terminal amphipathic helix. This binding facilitates the recruitment of the Nup107-160 complex, a crucial structural component of the NPC, to assembly sites. Our work further suggests that the nuclear transport receptor transportin and the small GTPase Ran regulate the interaction of Nup153 with the membrane and, in this way, direct pore complex assembly to the nuclear envelope during interphase.
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Vollmer
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Lorenz
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Moreno-Andrés
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Mona
authors.initials: M
authors.lastname: Bodenhöfer
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Paola
authors.initials: P
authors.lastname: De Magistris
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Susanne Adina
authors.initials: SA
authors.lastname: Astrinidis
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
authors.forename: Allana
authors.initials: A
authors.lastname: Schooley
authors.affiliation: Max Planck Institute for Developmental Biology, Spemannstraße 37, 72076 Tübingen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Flötenmeyer
authors.affiliation: Institute for Microbiology and Molecular Biology, University of Hohenheim, Garbenstraße 30, 70599 Stuttgart, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Leptihn
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany. Electronic address: wolfram.antonin@tuebingen.mpg.de.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Antonin
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D028884:Karyopherins / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009685:Nuclear Envelope / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis; D020931:ran GTP-Binding Protein / Q000378:metabolism
chemical_list: D028884:Karyopherins; C512772:NUP107 protein, human; C079377:NUP153 protein, human; C440251:NUP160 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; C519169:Nup153 protein, Xenopus; C499080:Nup160 protein, Xenopus; D011994:Recombinant Proteins; D029867:Xenopus Proteins; D020931:ran GTP-Binding Protein