title: High-quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols.
abstract: Due to the presence of a diverse array of metabolites, no standard method of RNA isolation is available for plants. We noted that polysaccharide and polyphenol contents of cardamom tissues critically hinder the RNA extraction procedure. Hence, we attempted several methods for obtaining intact mRNA and small RNA from various cardamom tissues. It was found that protocols involving a combination of commercial kits and conventional CTAB (cetyl trimethylammonium bromide) methods yielded RNA with good purity, higher yield, and good integrity. The total RNA isolated through this approach was found to be amenable for transcriptome and small RNA analysis through next-generation sequencing platforms.
authors.affiliation: Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI), Thiruvananthapuram 695562, Kerala, India.
authors.forename: Fasiludeen
authors.initials: F
authors.lastname: Nadiya
authors.affiliation: Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI), Thiruvananthapuram 695562, Kerala, India.
authors.forename: Narayanannair
authors.initials: N
authors.lastname: Anjali
authors.affiliation: Department of Botany, University of Kerala, Thiruvananthapuram 695037, Kerala, India.
authors.forename: Appukuttannair
authors.initials: A
authors.lastname: Gangaprasad
authors.affiliation: Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI), Thiruvananthapuram 695562, Kerala, India. Electronic address: sabu@jntbgri.res.in.
authors.forename: Kalluvettankuzhy Krishnannair
authors.initials: KK
authors.lastname: Sabu
mesh_terms: D028523:Elettaria / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry / Q000302:isolation & purification*; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D059808:Polyphenols; D011134:Polysaccharides; D012333:RNA, Messenger; D018749:RNA, Plant
keywords: Next-generation sequencing (NGS); RIN value; RNA isolation; mRNA; miRNA