title: Hypoxia inducible factor 1α expression and effects of its inhibitors in canine lymphoma.
abstract: Hypoxic conditions in various cancers are believed to relate with their malignancy, and hypoxia inducible factor-1α (HIF-1α) has been shown to be a major regulator of the response to low oxygen. In this study, we examined HIF-1α expression in canine lymphoma using cell lines and clinical samples and found that these cells expressed HIF-1α. Moreover, the HIF-1α inhibitors, echinomycin, YC-1 and 2-methoxyestradiol, suppressed the proliferation of canine lymphoma cell lines. In a xenograft model using NOD/scid mice, echinomycin treatment resulted in a dose-dependent regression of the tumor. Our results suggest that HIF-1α contributes to the proliferation and/or survival of canine lymphoma cells. Therefore, HIF-1α inhibitors may be potential agents to treat canine lymphoma.
authors.affiliation: Laboratory of Veterinary Internal Medicine, The United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kambayashi
authors.forename: Masaya
authors.initials: M
authors.lastname: Igase
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Ayana
authors.initials: A
authors.lastname: Kimura
authors.forename: Takako
authors.initials: T
authors.lastname: Shimokawa Miyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Baba
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Noguchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004283:Dog Diseases; D004285:Dogs; D004448:Echinomycin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism / Q000662:veterinary*; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D004448:Echinomycin