title: Photodegradable Gelatin-Based Hydrogels Prepared by Bioorthogonal Click Chemistry for Cell Encapsulation and Release.
abstract: In this study, we present a method for the fabrication of in situ forming gelatin and poly(ethylene glycol)-based hydrogels utilizing bioorthogonal, strain-promoted alkyne-azide cycloaddition as the cross-linking reaction. By incorporating nitrobenzyl moieties within the network structure, these hydrogels can be designed to be degradable upon irradiation with low intensity UV light, allowing precise photopatterning. Fibroblast cells encapsulated within these hydrogels were viable at 14 days and could be readily harvested using a light trigger. Potential applications of this new class of injectable hydrogel include its use as a 3D culturing platform that allows the capture and release of cells, as well as light-triggered cell delivery in regenerative medicine.
authors.affiliation: †Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton 3800 Victoria, Australia.
authors.forename: Vinh X
authors.initials: VX
authors.lastname: Truong
authors.affiliation: †Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton 3800 Victoria, Australia.|‡CSIRO Manufacturing Flagship, Clayton 3168 Victoria, Australia.|§CRC for Polymers, Notting Hill 3168 Victoria, Australia.
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Tsang
authors.affiliation: †Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton 3800 Victoria, Australia.
authors.forename: George P
authors.initials: GP
authors.lastname: Simon
authors.affiliation: ∥Anatomy and Developmental Biology, Monash Institute of Medical Engineering, Monash University, Clayton 3800 Victoria, Australia.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Boyd
authors.affiliation: ‡CSIRO Manufacturing Flagship, Clayton 3168 Victoria, Australia.|§CRC for Polymers, Notting Hill 3168 Victoria, Australia.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Evans
authors.affiliation: ‡CSIRO Manufacturing Flagship, Clayton 3168 Victoria, Australia.|§CRC for Polymers, Notting Hill 3168 Victoria, Australia.
authors.forename: Helmut
authors.initials: H
authors.lastname: Thissen
authors.affiliation: †Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton 3800 Victoria, Australia.
authors.forename: John S
authors.initials: JS
authors.lastname: Forsythe
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D060846:Cell Engineering; D002478:Cells, Cultured; D057930:Click Chemistry / Q000379:methods; D061565:Cycloaddition Reaction / Q000379:methods; D005347:Fibroblasts / Q000166:cytology*; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D010782:Photolysis
chemical_list: D020100:Hydrogels; D005780:Gelatin