title: Neuroprotective effect of epigallocatechin-3-gallate in a mouse model of chronic glaucoma.
abstract: Epigallocatechin-3-gallate (EGCG) is a powerful antioxidant with suggested neuroprotective action. This study investigated the protective effects of EGCG against retinal ganglion cells (RGCs) degeneration in an animal model of glaucoma. C57BL/6J mice (n=54) were divided randomly into four groups: normal control group (group A, n=12); EGCG control group with EGCG in drinking water (group B, n=12); microbeads control group with anterior chamber microbeads injection to induce elevation in intraocular pressure (IOP) plus normal drinking water (group C, n=18); and EGCG study group receiving an anterior chamber microbeads injection plus EGCG in drinking water (group D, n=12). Animals were treated orally with either vehicle or EGCG (50mg/kg×d). IOP was measured and animals were sacrificed at days 15 and 27. Neurons were retrograde-labeled by fluorogold and immnunolabeled by class III β-tubulin to quantify RGCs in the retinal ganglion cell layer on flat mounts histologically and compared. All mice that received microbeads injections (groups C and D) developed IOP elevation higher than un-injected control mice. At days 15 and 27, progressive loss of RGCs was observed after microbeads injection in group C (P<0.01). In contrast, the fluorogold-labeled RGC density and class III β-tubulin-positive RGC density were significantly higher in group D as compared to group C (P<0.01) but significantly lower than group B (P<0.01). These parameters did not differ significantly between groups A and B (P>0.05). The findings suggest the consumption of EGCG plays a neuroprotective role on RGCs in a mouse model of elevated IOP.
authors.affiliation: Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: Ceying
authors.initials: C
authors.lastname: Shen
authors.affiliation: Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China. Electronic address: jlbjlb@sina.com.
authors.forename: Libin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Hong Kong Eye Hospital, Kowloon, Hong Kong; Retina & Macula Centre, Kowloon, Hong Kong.
authors.forename: Timothy Y Y
authors.initials: TY
authors.lastname: Lai
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D002908:Chronic Disease; D005260:Female; D005901:Glaucoma / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D007429:Intraocular Pressure / Q000187:drug effects; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000473:pathology
chemical_list: D018696:Neuroprotective Agents; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Epigallocatechin-3-gallate; Glaucoma; High intraocular pressure; Neuroprotection; Retinal ganglion cells