title: 10-Hydroxy-2-decenoic acid inhibiting the proliferation of fibroblast-like synoviocytes by PI3K-AKT pathway.
abstract: To reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Different genes in FLS cells from RA patients were primary cultured and treated with 10H2DA. They were then screened by Human Transcriptome 1.0 ST microarrays and verified by real-time PCR. The results showed dose-dependent and time-dependent decreases in cell viability and HDAC activity in FLSs treated with 10H2DA, and time-dependent induction in the acetylation of H3 and H4 at the same time. 697 different genes were identified by HTA 1.0. The expressions of 7 target genes of the PI3K-AKT pathway were decreased and 4 target genes of cytokine-cytokine receptor interaction were increased verified by real-time PCR. These results imply that 10H2DA is a potential HDACI which inhibits the proliferation of FLS cells by PI3K-AKT pathway.
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zou
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Lin
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Qiang
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Chifundo Martha
authors.initials: CM
authors.lastname: Chuka
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Renshan
authors.initials: R
authors.lastname: Ge
authors.affiliation: Department of Marine Biology, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China. Electronic address: zsj62@163.com.
authors.forename: Shujiang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China. Electronic address: cyrillyoung@163.com.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Yang
mesh_terms: D000107:Acetylation; D001172:Arthritis, Rheumatoid / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D051058:Oncogene Protein v-akt / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases / Q000187:drug effects*; D061251:Primary Cell Culture; D018121:Receptors, Cytokine / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects*; D013582:Synovial Fluid / Q000166:cytology* / Q000187:drug effects*
chemical_list: D016207:Cytokines; D005229:Fatty Acids, Monounsaturated; D006657:Histones; D018121:Receptors, Cytokine; C055543:10-hydroxy-2-decenoic acid; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D006655:Histone Deacetylases
keywords: 10H2DA; HDACIs; Microarray; PI3K–AKT pathway; Rheumatoid arthritis