title: Weight gain and inflammation regulate aromatase expression in male adipose tissue, as evidenced by reporter gene activity.
abstract: Obesity and white adipose tissue (WAT) inflammation are associated with enhanced aromatization in women, but little is known about the regulation of aromatase (CYP19A1) gene expression in male WAT. We investigated the impact of weight gain and WAT inflammation on the regulation of CYP19A1 in males, by utilizing the hARO-Luc aromatase reporter mouse model containing a >100-kb 5'-region of the human CYP19A1 gene. We show that hARO-Luc reporter activity is enhanced in WAT of mice with increased adiposity and inflammation. Dexamethasone and TNFα, as well as forskolin and phorbol 12-myristate 13-acetate, upregulate hARO-Luc activity, suggesting the involvement of promoters I.4 and I.3/II. Furthermore, we show that diet enriched with antioxidative plant polyphenols attenuates WAT inflammation and hARO-Luc activity in obese males. In conclusion, our data suggest that obesity-associated WAT inflammation leads to increased peripheral CYP19A1 expression in males, and that polyphenol-enriched diet may have the potential to attenuate excessive aromatization in WAT of obese men.
authors.affiliation: Functional Foods Forum, University of Turku, Turku, Finland; Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Polari
authors.affiliation: Functional Foods Forum, University of Turku, Turku, Finland; Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland.
authors.forename: E
authors.initials: E
authors.lastname: Yatkin
authors.affiliation: Functional Foods Forum, University of Turku, Turku, Finland; Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland.
authors.forename: M G
authors.initials: MG
authors.lastname: Martínez Chacón
authors.affiliation: Research Centre of Applied and Preventive Cardiovascular Medicine, University of Turku, Turku, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Ahotupa
authors.affiliation: Åbo Akademi University, Process Chemistry Centre, Laboratory of Wood and Paper Chemistry, Turku, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Smeds
authors.affiliation: Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland; Department of Physiology, Institute of Biomedicine, University of Turku, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Strauss
authors.affiliation: Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland; Department of Physiology, Institute of Biomedicine, University of Turku, Finland.
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland; Department of Physiology, Institute of Biomedicine, University of Turku, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Poutanen
authors.affiliation: Functional Foods Forum, University of Turku, Turku, Finland; Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland; Department of Physiology, Institute of Biomedicine, University of Turku, Finland.
authors.forename: N
authors.initials: N
authors.lastname: Saarinen
authors.affiliation: Functional Foods Forum, University of Turku, Turku, Finland; Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, Turku, Finland. Electronic address: sarmak@utu.fi.
authors.forename: S I
authors.initials: SI
authors.lastname: Mäkelä
mesh_terms: D052436:Adipose Tissue, White / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004790:Enzyme Induction; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D009765:Obesity / Q000209:etiology / Q000276:immunology / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage; D059808:Polyphenols / Q000008:administration & dosage; D015533:Transcriptional Activation; D015430:Weight Gain
chemical_list: D000975:Antioxidants; D016207:Cytokines; D010936:Plant Extracts; D059808:Polyphenols; D008156:Luciferases; D001141:Aromatase
keywords: Aromatase; Inflammation; Lignans; Obesity; Polyphenols; White adipose tissue