title: Development of Lung Epithelium from Induced Pluripotent Stem Cells.
abstract: Considerable progress has been made in the field ofin vitrodevelopment of alveolar epithelium from induced pluripotent stem cells. Patient specific derived alveolar cells could potentially populate tissue engineered lungs, provide a cell source for drug testing or function as a model for research into lung diseases. Induced to pluripotency through a variety of techniques, stem cells can be differentiated to alveolar epithelium through exposure to a variety of different culture conditions and growth media. The ultimate success of differentiated cells for translational medicine applications will depend on further advances in the understanding of the human lung developmental pathway, and successful application toin vitroculture. In this review will focus the major signaling pathways and molecules in lung development and the existing protocol for directed different ion of iPSC and hESC to cells resembling respiratory epitheliumin vitro.
authors.affiliation: Departments of Anesthesia and Biomedical Engineering, Yale University, New Haven, Connecticut, 06520, USA.
authors.forename: Mahboobe
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Departments of Anesthesia and Biomedical Engineering, Yale University, New Haven, Connecticut, 06520, USA.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Niklason
authors.affiliation: Departments of Anesthesia and Biomedical Engineering, Yale University, New Haven, Connecticut, 06520, USA.
authors.forename: Jordana
authors.initials: J
authors.lastname: Williams
keywords: Pluripotent stem cells; differentiation; lung epithelial cells