title: Isolation of Brucella melitensis from a human case of chronic additive polyarthritis.
abstract: Brucellar arthritis remains under diagnosed owing to non-specific clinical manifestations. Here, we report isolation of Brucella melitensis from synovial fluid of 5th metatarsophalangeal joint of a 39-year-old lady having unusually chronic asymmetric, additive, peripheral polyarthritis. This isolation was confirmed by Bruce-Ladder polymerase chain reaction (PCR). The patient had a history of contact with an aborted goat. Rose Bengal Plate Agglutination Test (RBPT) and Standard Tube Agglutination Test (SAT) were positive for Brucella-specific antibodies both for patient and in contact with sheep and goats. The patient was treated with doxycycline and rifampicin for 16 weeks and was recovered fully.
authors.affiliation: Department of Veterinary Microbiology, Dr. GC Negi College of Veterinary and Animal Sciences, Chaudhary Sarwan Kumar Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, India.
authors.forename: R
authors.initials: R
authors.lastname: Chahota
authors.forename: A
authors.initials: A
authors.lastname: Dattal
authors.forename: S D
authors.initials: SD
authors.lastname: Thakur
authors.forename: M
authors.initials: M
authors.lastname: Sharma
mesh_terms: D000328:Adult; D000372:Agglutination Tests; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000907:Antibodies, Bacterial / Q000097:blood; D001168:Arthritis / Q000188:drug therapy / Q000209:etiology* / Q000382:microbiology / Q000473:pathology*; D017347:Brucella melitensis / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology*; D002908:Chronic Disease; D004318:Doxycycline / Q000627:therapeutic use; D005260:Female; D006041:Goats / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000627:therapeutic use; D012756:Sheep / Q000382:microbiology; D013582:Synovial Fluid / Q000382:microbiology*; D016896:Treatment Outcome; D015047:Zoonoses / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D004318:Doxycycline; D012293:Rifampin