title: A highly selective turn-on fluorescent probe for hypochlorous acid based on hypochlorous acid-induced oxidative intramolecular cyclization of boron dipyrromethene-hydrazone.
abstract: A BODIPY-based fluorescent probe, HBP, was developed for the detection of hypochlorous acid based on the specific hypochlorous acid-promoted oxidative intramolecular cyclization of heterocyclic hydrazone in response to the amount of HOCl. The reaction is accompanied by a 41-fold increase in the fluorescent quantum yield (from 0.004 to 0.164). The fluorescence intensity of the reaction between HOCl and HBP is linear in the HOCl concentration range of 1-8 μM with a detection limit of 2.4 nM (S/N=3). Confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe HBP could be used as an effective fluorescent probe for detecting HOCl in living cells.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan, ROC.
authors.forename: Wei-Chieh
authors.initials: WC
authors.lastname: Chen
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan, ROC.
authors.forename: Parthiban
authors.initials: P
authors.lastname: Venkatesan
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan, ROC. Electronic address: spwu@mail.nctu.edu.tw.
authors.forename: Shu-Pao
authors.initials: SP
authors.lastname: Wu
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002460:Cell Line; D003500:Cyclization; D005456:Fluorescent Dyes / Q000737:chemistry*; D006835:Hydrazones / Q000737:chemistry*; D006997:Hypochlorous Acid / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D051379:Mice; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D006835:Hydrazones; D006997:Hypochlorous Acid
keywords: Bioimaging; Boron dipyrromethene; Fluorescent probe; Hypochlorous acid