title: Purification and characterisation of a hypoglycemic peptide from Momordica Charantia L. Var. abbreviata Ser.
abstract: A water-soluble peptide MC2-1-5 from Momordica charantia L. Var. Abbreviata Ser., with hypoglycemic effect, was purified by ultrafiltration, gel filtration chromatography and reverse-phase high performance liquid chromatography (RP-HPLC). The infrared (IR) spectra showed characteristic absorption peaks and the molecular mass of MC2-1-5 was found to be 3405.5174Da by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The sequence of its first 10 N-terminal amino acids was GHPYYSIKKS as determined by a protein sequencer. MC2-1-5 reduced the blood glucose level in alloxan-induced diabetic mice by 61.70% and 69.18% at 2 and 4h, respectively, after oral administration at a dose of 2mg/kg. The oral glucose tolerance test (OGTT) showed MC2-1-5 produced a reduction of 25.50%, 39.62% and 41.74% in blood glucose level after 1, 2 and 3h, respectively, of oral administration compared with a diabetic control.
authors.affiliation: College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China. Electronic address: yxq1977@hotmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
keywords: Alloxan; Diabetes mellitus; Hypoglycemic effect; Momordica charantia L. Var. abbreviata Ser.; Peptide