title: Depolymerization of polysaccharides from Opuntia ficus indica: Antioxidant and antiglycated activities.
abstract: The extraction, purification and degradation of polysaccharides from Opuntia ficus indica cladodes, as well as the evaluation of their antioxidant and antiglycated activities in vitro were investigated. The optimization of the extraction showed that extraction by ultrasound at 40 °C presented the best carbohydrates yield. The degradation of the extracted polysaccharides was achieved by free radical depolymerization with H2O2 in the presence of copper(II) acetate for various reaction times. Sugar contents were determined by colorimetric assays. The macromolecular characteristics of the different isolated and degraded carbohydrates were carried by size exclusion chromatography (SEC/MALS/VD/DRI). These experiments showed that all samples are polysaccharides, which are probably pectins and that molecular weight (Mw) has decreased from 6,800,000 to 14,000 g/mol after 3 h of depolymerization without changing the structure. Preliminary antioxidant and antiglycated tests indicated that degraded polysaccharides for 2 and 3 h showed even better antioxidant and antiglycated activities.
authors.affiliation: Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia.
authors.forename: Mohamed Aymen
authors.initials: MA
authors.lastname: Chaouch
authors.affiliation: Laboratoire de Biochimie, Faculté de Médecine, Université de Sousse, 4002 Sousse, Tunisia.
authors.forename: Jawhar
authors.initials: J
authors.lastname: Hafsa
authors.affiliation: Normandie Université, Laboratoire Polymères Biopolymères Surfaces (PBS), UMR 6270 & FR 3038 CNRS, Université de Rouen, 76821 Mont Saint Aignan, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Rihouey
authors.affiliation: Normandie Université, Laboratoire Polymères Biopolymères Surfaces (PBS), UMR 6270 & FR 3038 CNRS, Université de Rouen, 76821 Mont Saint Aignan, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Le Cerf
authors.affiliation: Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia. Electronic address: hatemmajdoub.fsm@gmail.com.
authors.forename: Hatem
authors.initials: H
authors.lastname: Majdoub
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002417:Cattle; D002850:Chromatography, Gel; D006031:Glycosylation; D006861:Hydrogen Peroxide / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation; D029747:Opuntia / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D010851:Picrates / Q000037:antagonists & inhibitors; D018547:Plant Stems / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D013010:Sonication; D013696:Temperature
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D009942:Organometallic Compounds; D010851:Picrates; D012996:Solutions; D012710:Serum Albumin, Bovine; C015092:cupric acetate; D010368:Pectins; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antiglycation activity; Antioxidant activity; Free radical depolymerization; Opuntia ficus indica; Polysaccharide