title: Functional properties of fish protein hydrolysates from Pacific whiting(Merluccius productus) muscle produced by a commercial protease.
abstract: Pacific whiting (Merluccius productus) muscle was used to produce hydrolysates with 10%, 15% and 20% degree of hydrolysis (DH) using the commercial protease Alcalase(®) and were characterized at pH 4.0, 7.0 and 10 according their solubility, emulsifying and foaming properties. Protein recovered in soluble fractions increased proportionally with the hydrolytic process, yielded 48.6±1.9, 58.6±4.1 and 67.8±1.4 of total protein after 10%, 15% and 20% DH, respectively. Freeze-dried hydrolysates presented almost 100% solubility (p>0.05) at the different pHs evaluated. Emulsifying properties (EC, EAI and ESI) were not affected by DH as most samples showed similar (p>0.05) results. Higher EC (p⩽0.05) than sodium caseinate, used as control, were obtained at pH 4 for most hydrolysates. Hydrolysates showed very low foaming capacity not affected by pH; but foam stability was equal or even better (p>0.05) than bovine serum albumin (BSA), except at pH 4.0. Results suggest that hydrolysates from Pacific whiting muscle can be produced with similar or better functional properties than the food ingredients used as standards.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD, A.C.) Carretera al Ejido La Victoria, Km 0.6, Apdo. Postal 1735, Col. Centro,CP 83000 Hermosillo, Sonora, Mexico.
authors.forename: Ramón
authors.initials: R
authors.lastname: Pacheco-Aguilar
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD, A.C.) Carretera al Ejido La Victoria, Km 0.6, Apdo. Postal 1735, Col. Centro,CP 83000 Hermosillo, Sonora, Mexico.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Mazorra-Manzano
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD, A.C.) Carretera al Ejido La Victoria, Km 0.6, Apdo. Postal 1735, Col. Centro,CP 83000 Hermosillo, Sonora, Mexico. Electronic address: jcramirez@ciad.mx.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Ramírez-Suárez
keywords: Emulsifying capacity; Fish protein hydrolysates; Foaming capacity; Functional characterization; Pacific whiting; Proteolytic activity