title: Diffusion and persistence of multidrug resistant Salmonella Typhimurium strains phage type DT120 in southern Italy.
abstract: Sixty-two multidrug resistant Salmonella enterica serovar Typhimurium strains isolated from 255 clinical strains collected in Southern Italy in 2006-2008 were characterised for antimicrobial resistance genes, pulsotype, and phage type. Most strains (83.9%) were resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline (ACSSuT) encoded in 88.5% by the Salmonella genomic island (SGI1) and in 11.5% by the InH-like integron (bla OXA-30-aadA1) and catA1, sul1, and tet(B) genes. STYMXB.0061 (75%) and DT120 (84.6%) were the prevalent pulsotype and phage type identified in these strains, respectively. Five other resistance patterns were found either in single or in a low number of isolates. The pandemic clone DT104 (ACSSuT encoded by SGI1) has been identified in Italy since 1992, while strains DT120 (ACSSuT encoded by SGI1) have never been previously reported in Italy. In Europe, clinical strains DT120 have been reported from sporadic outbreaks linked to the consumption of pork products. However, none of these strains were STYMXB.0061 and SGI1 positive. The prevalent identification and persistence of DT120 isolates would suggest, in Southern Italy, a phage type shifting of the pandemic DT104 clone pulsotype STYMXB.0061. Additionally, these findings raise epidemiological concern about the potential diffusion of these emerging multidrug resistant (SGI linked) DT120 strains.
authors.affiliation: Department of Basic Medical Sciences Neurosciences and Sense Organs Medical Faculty, University of Bari Piazza G. Cesare Policlinico, 70124 Bari, Italy.
authors.forename: Danila
authors.initials: D
authors.lastname: De Vito
authors.affiliation: Department of Basic Medical Sciences Neurosciences and Sense Organs Medical Faculty, University of Bari Piazza G. Cesare Policlinico, 70124 Bari, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Monno
authors.affiliation: Department of Biology, University of Bari "Aldo Moro", Via E. Orabona 4, 70125 Bari, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Nuccio
authors.affiliation: Department of Biology, University of Bari "Aldo Moro", Via E. Orabona 4, 70125 Bari, Italy.
authors.forename: Marilisa
authors.initials: M
authors.lastname: Legretto
authors.affiliation: Department of Biology, University of Bari "Aldo Moro", Via E. Orabona 4, 70125 Bari, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Oliva
authors.affiliation: Department of Basic Medical Sciences Neurosciences and Sense Organs Medical Faculty, University of Bari Piazza G. Cesare Policlinico, 70124 Bari, Italy.
authors.forename: Maria Franca
authors.initials: MF
authors.lastname: Coscia
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, National Health Institute, 00161 Rome, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Dionisi
authors.affiliation: Department of Basic Medical Sciences Neurosciences and Sense Organs Medical Faculty, University of Bari Piazza G. Cesare Policlinico, 70124 Bari, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Calia
authors.affiliation: Department of Basic Medical Sciences Neurosciences and Sense Organs Medical Faculty, University of Bari Piazza G. Cesare Policlinico, 70124 Bari, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Capolongo
authors.affiliation: Department of Biology, University of Bari "Aldo Moro", Via E. Orabona 4, 70125 Bari, Italy.
authors.forename: Carlo
authors.identifier: 0000-0003-0414-2965
authors.initials: C
authors.lastname: Pazzani
mesh_terms: D001434:Bacteriophage Typing; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D044404:Genomic Islands; D006801:Humans; D032023:Integrons; D007558:Italy; D012480:Salmonella Infections / Q000382:microbiology; D012483:Salmonella Phages / Q000145:classification / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000302:isolation & purification / Q000821:virology*
chemical_list: D004269:DNA, Bacterial