title: Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression.
abstract: Psoriasis is mediated primarily by T cells, which reduce epidermal turnover time and affect keratinocyte proliferation. We aimed to identify differentially expressed genes (DEG) in T cells from normal, five pairs of monozygotic twins concordant or discordant for psoriasis, to determine whether these DEG may account for the influence to epidermal turnover time and keratinocyte proliferation. The impact of T cells on keratinocyte proliferation and epidermal turnover time were investigated separately by immunohistochemistry and cultured with (3) H-TdR. mRNA expression patterns were investigated by RNA sequencing and verified by real-time reverse transcription polymerase chain reaction. After co-culture with psoriatic T cells, the expression of Ki-67, c-Myc and p53 increased, while expression of Bcl-2 and epidermal turnover time decreased. There were 14 DEG which were found to participate in the regulation of cell proliferation or differentiation. Psoriatic T cells exhibited the ability to decrease epidermal turnover time and affect keratinocyte proliferation because of the differential expression of PPIL1, HSPH1, SENP3, NUP54, FABP5, PLEKHG3, SLC9A9 and CHCHD4.
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Junqin
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Changzhi City Second People's Hospital, Changzhi, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Dong
authors.affiliation: Laboratory Animal Center, Shanxi Medical University, Taiyuan, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yin
authors.affiliation: Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000942:Antigens, Bacterial; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D049109:Cell Proliferation; D004817:Epidermis / Q000503:physiopathology; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000502:physiology; D019394:Ki-67 Antigen / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D011565:Psoriasis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D013601:T-Lymphocytes / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D019394:Ki-67 Antigen; C489427:MYC protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; C117321:streptococcal superantigen SSA
keywords: T cells; differentially expressed genes; epidermal turnover time; keratinocyte; psoriasis