title: Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate.
abstract: Alfalfa leaf protein, a potential source of high quality protein for human consumption, was hydrolyzed with protease. Alfalfa leaf protein hydrolysate was fractionated by ultrafiltration and the obtained peptides were purified by dynamical adsorption. The antioxidant activity of alfalfa leaf peptides (ALPs) was investigated and compared with that of a native antioxidant, reduced glutathione (GSH), which was used as a reference. The reducing power of ALPs was 0.69 at 2.00mg/mL. ALPs at 1.60mg/mL and 0.90mg/mL exhibited 79.71% and 67.00% of scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide radical, respectively. In addition, ALPs showed 65.15% chelating effect on ferrous ion at 0.50mg/mL. The molecular weight of the peptides was determined and 67.86% of the total amount was below 1000Da. Combined with the result of the amino acid profiles, ALPs was believed to have high nutritive value in addition to antioxidant activity.
authors.affiliation: School of Food Science and Technology, JiangNan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu Province, China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Xie
authors.affiliation: College of Life Science and Engineering, Shaanxi University of Science and Technology, Weiyang District, 710021 Xi'an, Shaanxi Province, China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Food Science and Technology, JiangNan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu Province, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Food Science and Technology, JiangNan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu Province, China. Electronic address: zjin@jiangnan.edu.cn.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jin
keywords: Alfalfa leaf peptides; Amino acid; Antioxidant; Free radical; Molecular weight