title: Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum.
abstract: A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Tykistokatu 6, 20521 Turku, Finland.
authors.forename: Anastassios C
authors.initials: AC
authors.lastname: Papageorgiou
authors.affiliation: Department of Environmental Biology, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
authors.forename: Duochuan
authors.initials: D
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D002597:Chaetomium / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D043326:Glucan 1,3-beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D005810:Multigene Family; D010710:Phosphates / Q000737:chemistry; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D017680:Potassium Compounds / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D010710:Phosphates; D017680:Potassium Compounds; D011994:Recombinant Proteins; C013216:potassium phosphate; D043326:Glucan 1,3-beta-Glucosidase; C018279:sodium phosphate
keywords: cellulose; glucanase; in situ diffraction; seeding; thermophilic fungus