title: Steady-state cross-correlations for live two-colour super-resolution localization data sets.
abstract: Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.
authors.affiliation: Department of Biophysics, University of Michigan, 930 N University, Ann Arbor, Michigan 48109, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Stone
authors.affiliation: Department of Biophysics, University of Michigan, 930 N University, Ann Arbor, Michigan 48109, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Veatch
mesh_terms: D001402:B-Lymphocytes / Q000737:chemistry; D002460:Cell Line; D003116:Color; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins