title: A new mouse model for wound healing in hemophilia A.
abstract: OBJECTIVE
  To establish a new mouse model for wound healing studies on hemophilia A.
  
  
  METHODS
  Total 54 male mice with different genotypes including wild-type nude mice, heterozygous mice (FVIII-/-/Nu) and FVIII deficient mice (FVIII-/-) were generated and verified by PCR. Mice were subjected to wound healing research by making a 5 mm-thickness wound on mice skin and applying recombinant human epidermal growth factor (EGF, 10 μg/g) ointment, FVIII ointment (30 IU) or the ointment base to heal the wounds. Furthermore, keratinocytes were isolated from these newborn mice and subjected to migration assay by stimulation of EGF (ng/ml), insulin (10 μM) or vehicle.
  
  
  RESULTS
  A new hemophilic mouse model (FVIII-/-/Nu) was constructed successfully after genotyping verified by PCR. Compared to FVIII-/- mice, FVIII-/-/Nu and Nu mice showed greater degree of wound contraction and loss of the crust. Topical treatment with EGF exhibited faster wound healing than FVIII and ointment base. Insulin treatment showed more increased migration distance than treated with EGF or vehicle. FVIII-/-/Nu mice showed greater migration than FVIII-/- and Nu mice.
  
  
  CONCLUSIONS
  A new mouse model (FVIII-/-/Nu) for wound healing in hemophilia A was constructed, and topical treatment of insulin may be a better therapy than EGF for healing wounds in hemophilia A.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Gao
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors.forename: Dhahiri Saidi
authors.initials: DS
authors.lastname: Mashausi
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors.forename: Hema
authors.initials: H
authors.lastname: Negi
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006467:Hemophilia A; D051379:Mice; D008817:Mice, Mutant Strains; D016133:Polymerase Chain Reaction; D014945:Wound Healing
keywords: Wound healing; insulin; keratinocytes; recombinant human epidermal growth factor