title: MAGEA10 gene expression in non-small cell lung cancer and A549 cells, and the affinity of epitopes with the complex of HLA-A(∗)0201 alleles.
abstract: MAGEA10, a cancer/testis antigens expressed in tumors but not in normal tissues with the exception of testis and placenta, represents an attractive target for cancer immunotherapy. However, suppressive cytoenvironment and requirement of specific HLA-alleles presentation frequently led to immunotherapy failure. In this study MAGEA10 was scarcely expressed in cancer patients, but enhanced by viili polysaccharides, which indicates a possibility of increasing epitopes presentation. Furthermore the correlation of gene expression with methylation, indicated by R(2) value for MAGEA10 that was 3 times higher than the value for other MAGE genes tested, provides an explanation of why MAGEA10 was highly inhibited, this is also seen by Kaplan-Meier analysis because MAGEA10 did not change the patients' lifespan. By using Molecular-Docking method, 3 MAGEA10 peptides were found binding to the groove position of HLA-A(∗)0210 as same as MAGEA4 peptide co-crystallized with HLA-A(∗)0210, which indicates that they could be promising for HLA-A(∗)0201 presentation in immunotherapy.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China. Electronic address: wonalbert69@gmail.com.
authors.forename: Likui
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of pharmacy, First Hospital Affiliated to PLA General Hospital, Beijing 100037, PR China. Electronic address: xyf304@126.com.
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Biotechnology, University of Tartu, Riia 23, Tartu 51010, Estonia. Electronic address: Luo58@yahoo.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Luo
authors.affiliation: Department of Chemistry, The University of Hong Kong, Hong Kong. Electronic address: Sunjian.nku@gmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, PR China. Electronic address: jlz20062621@163.com.
authors.forename: Jinlu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Medical Laboratory and Biotechnology, Chung-Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City 402, Taiwan. Electronic address: d880430@csmu.edu.tw.
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Lee
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02215, USA. Electronic address: baiap@163.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Bai
authors.affiliation: Department of Chemistry, The University of Hong Kong, Hong Kong. Electronic address: ghc@everest.hku.hk.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Mathematics and Computer Science, Mercy College, Dobbs Ferry, NY, USA. Electronic address: cfenz@gmail.com.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Frenz
authors.affiliation: School of Life Science, Chongqing University, Chongqing 400030, China. Electronic address: zhengguoli@cqu.edu.cn.
authors.forename: Zhengguo
authors.initials: Z
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China. Electronic address: wl_huang@hotmail.com.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Huang
mesh_terms: D000483:Alleles; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000276:immunology*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D008175:Lung Neoplasms / Q000276:immunology / Q000378:metabolism*; D062105:Molecular Docking Simulation; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D018984:Epitopes, T-Lymphocyte; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C121012:MAGEA10 protein, human; C095204:MAGEA4 protein, human; D009363:Neoplasm Proteins
keywords: Cancer/tissue antigen (CTA); Epitopes; Gene expression/methylation; HLA-A(∗)0201; MAGEA10