title: MicroRNA-429 inhibits the migration and invasion of colon cancer cells by targeting PAK6/cofilin signaling.
abstract: MicroRNAs (miRs), a class of non-coding RNAs 18-25 nucleotides in length, can lead to mRNA degradation or inhibit protein translation by directly binding to the 3'-untranslational region (UTR) of their target mRNAs. The deregulation of miR-429 has been suggested to be involved in the development and progression of colon cancer. However, the detailed molecular mechanism involved remains to be determined. The aim of the present study was to investigate the role of miR-429 in the regulation of migration and invasion of colon cancer cells using RT-qPCR and western blotting. The results showed that the expression of miR-429 was reduced in colon cancer cell lines, when compared to a normal colon epithelial cell line. Treatment with DNA demethylation agent 5-aza-2'-deoxycytidine and histone deacetylase inhibitor phenylbutyrate (PBA), or transfection with the pre-miR-429 lentivirus plasmid led to the upregulation of miR-429 expression, as well as inhibition of migration and invasion in colon cancer cells. Investigation of the molecular mechanism showed that PAK6 was a novel target of miR-429, and the expression of PAK6 was upregulated in colon cancer tissues and cell lines, and was negatively regulated by miR-429 in colon cancer cells. Moreover, the cofilin signaling acted as a downstream effector of miR-429 in colon cancer cells. In conclusion, the results of the present study suggested that miR-429 inhibits the migration and invasion of colon cancer cells, partly at least, by mediating the expression of PAK6, as well as the activity of cofilin signaling. Therefore, miR-429 is as a potential molecular target for the treatment of colon cancer.
authors.affiliation: Department of Oncology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Oncology, Peace Hospital of Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China.
authors.forename: Zibai
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Immunology, Peace Hospital of Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Oncology, Peace Hospital of Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Pathology, Peace Hospital of Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Oncology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Meizuo
authors.initials: M
authors.lastname: Zhong
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D051338:Cofilin 1 / Q000096:biosynthesis* / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D015398:Signal Transduction; D054462:p21-Activated Kinases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C495810:CFL1 protein, human; D051338:Cofilin 1; C546363:MIRN429 microRNA, human; D035683:MicroRNAs; C429007:PAK6 protein, human; D054462:p21-Activated Kinases