title: iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.).
abstract: Male sterility is a common phenomenon in flowering plants, and it has been successfully developed in several crops by taking advantage of heterosis. Cotton (Gossypium hirsutum L.) is an important economic crop, used mainly for the production of textile fiber. Using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant CCRI9106 was isolated from the wild-type upland cotton cultivar CCRI040029. To use CCRI9106 in cotton hybrid breeding, it is of great importance to study the molecular mechanisms of its male sterility. Here, histological and iTRAQ-facilitated proteomic analyses of anthers were performed to explore male sterility mechanisms of the mutant. Scanning and transmission electron microscopy of the anthers showed that the development of pollen wall in CCRI9106 was severely defective with a lack of exine formation. At the protein level, 6121 high-confidence proteins were identified and 325 of them showed differential expression patterns between mutant and wild-type anthers. The proteins up- or down-regulated in MT anthers were mainly involved in exine formation, protein degradation, calcium ion binding,etc. These findings provide valuable information on the proteins involved in anther and pollen development, and contribute to elucidate the mechanism of male sterility in upland cotton.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China.
authors.forename: Chaoyou
authors.initials: C
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China.
authors.forename: Hengling
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Song
authors.affiliation: Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South Central University for Nationalities, Wuhan 430064, Hubei Province, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Meng
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan Province, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China. Electronic address: fansl@cricaas.com.cn.
authors.forename: Shuli
authors.initials: S
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China. Electronic address: yu@cricaas.com.cn.
authors.forename: Shuxun
authors.initials: S
authors.lastname: Yu
mesh_terms: D003368:Gossypium / Q000235:genetics / Q000378:metabolism*; D051479:Plant Infertility; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D010940:Plant Proteins
keywords: Exine; Male sterility; Photosensitive; Pollen development; Proteome; Tapetum