title: Zingiber mioga (Thunb.) Roscoe attenuates allergic asthma induced by ovalbumin challenge.
abstract: Zingiber mioga (Thunb.) Roscoe (ZM) is a traditional medicine, used to treat inflammatory diseases. The present study aimed to evaluate the inhibitory effects of ZM on the inflammatory response in lipopolysaccharide (LPS)‑stimulated RAW264.7 murine macrophage cells and in a mouse model of ovalbumin (OVA)‑induced allergic asthma. Mice received OVA sensitization on day 0 and 14, and were challenged with OVA between days 21 and 23. ZM was administered to the mice at a dose of 30 mg/kg, 1 h prior to OVA challenge. In LPS‑stimulated RAW264.7 cells, ZM significantly decreased nitric oxide (NO) and tumor necrosis factor (TNF)‑α production in a concentration‑dependent manner, and mRNA expression of inducible NO synthase (iNOS), TNF‑α and matrix metalloproteinase (MMP)‑9 was reduced. In addition, treatment with ZM decreased the inflammatory cell count in bronchoalveolar lavage fluid from the mice, and reduced the expression of interleukin (IL)‑4, IL‑5, IL‑13, eotaxin and immunoglobulin E. ZM also reduced airway hyperresponsiveness in OVA‑challenged mice, and attenuated the infiltration of inflammatory cells and mucus production in the airways, with a decrease in the expression of iNOS and MMP‑9 in lung tissue. In conclusion, the results of the present study indicate that ZM effectively inhibits inflammatory responses. Therefore, it may be that ZM has potential as a therapeutic agent for use in inflammatory diseases.
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Na-Rae
authors.initials: NR
authors.lastname: Shin
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Shin
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Chan-Mi
authors.initials: CM
authors.lastname: Jeon
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Ju-Mi
authors.initials: JM
authors.lastname: Hong
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Hui-Seong
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.affiliation: Department of Biological Science and Biotechnology, Hannam University, Yuesong‑Gu, Daejeon 305‑811, Republic of Korea.
authors.forename: Kyu-Woung
authors.initials: KW
authors.lastname: Hahn
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Chungbuk 363‑883, Republic of Korea.
authors.forename: Kyung-Seop
authors.initials: KS
authors.lastname: Ahn
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000737:chemistry / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002460:Cell Line; D054413:Chemokine CCL11 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D005260:Female; D015870:Gene Expression; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology; D018793:Interleukin-13 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015848:Interleukin-5 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D010047:Ovalbumin / Q000009:adverse effects / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D018927:Anti-Asthmatic Agents; D054413:Chemokine CCL11; D018793:Interleukin-13; D015848:Interleukin-5; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D009569:Nitric Oxide; D007073:Immunoglobulin E; D010047:Ovalbumin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D020780:Matrix Metalloproteinase 9