title: Downregulation of microRNA-210 inhibits osteosarcoma growth in vitro and in vivo.
abstract: MicroRNA‑210 (miR‑210), the master hypoxamir, has various roles in the development of certain cancer types. It has been reported that miR‑210 expression was upregulated in patients with osteosarcoma (OS). However, little is known regarding its role in the development of human OS. In the present study, to explore the feasibility of miR‑210 as an effective therapeutic target, miR‑210 inhibitor was transfected into the osteosarcoma cell line MG‑63 cells, and cell proliferation, colony formation, cycle, apoptosis, migration and invasion were assessed. It was found that miR‑210 downregulation significantly suppressed clonogenicity, migration and invasion, as well as induced cell apoptosis, increased the percentage of cells in G1 phrase and decreased the percentage of cells in S phase in vitro. In addition, the effect of miR‑210 on tumor growth was evaluated in vivo. The results indicated that miR‑210 downregulation significantly suppressed tumor growth in nude mouse models. In conclusion, the findings of the present study suggested that miR‑210 is a potential therapeutic agent for the treatment of OS.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Changjian
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D015854:Up-Regulation
chemical_list: C543800:MIRN210 microRNA, human; C549325:MIRN210 microRNA, mouse; D035683:MicroRNAs