title: The establish of the HPLC method to examine the plasma concentration of lamotrigine and oxcarbazepine.
abstract: To establish the HPLC method to examine plasma concentration of lamotrigine and oxcarbazepine. This study set chlorzoxazone as the internal standard, chromatographic column was Column C18 (200×4.6mm, 5um) of DIKMA company, the mobile phase was methanol, water and trifluoroacetic acid, with rate of 40: 60: 0.0005, at a flow rate of 1 mllmin(-1), the detected wavelength was 240 nm. The plasma concentrations of lamotrigine was 0.5-50ug•mL(-1), the standard curve was excellent for Y=0.5511C-0.5669, r=0.9940, average recovery was 91.40%; The plasma concentrations of oxcarbazepine was 0.5-50ugmL-1, the standard curve was good for Y=0.4026C-0.5895, r=0.9925, and the average recovery was 89.59%; The three plasma concentrations of lamotrigine were respectively 25μg•mL(-1), 10 μg•mL(-1) and 2μg•mL(-1) and its five parallel sample for injection RSD were respectively 4.01%, 6.15% and 4.64%; The three plasma concentration of oxcarbazepine were 25μg•mL(-1)-1(-1), 10μg•mL(-1)-1(-1) and 2μg•mL(-1)-1(-1), and its five parallel sample for injection RSD were respectively 3.05%, 4.27% and 9.01%. This method was easy to operate, high recovery and high precision, and was applicable to the clinical detection for plasma concentration of lamotrigine and oxcarbazepine.
authors.affiliation: The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
authors.forename: Xue-Ping
authors.initials: XP
authors.lastname: Zhu
authors.affiliation: The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
authors.forename: Yao-Dong
authors.initials: YD
authors.lastname: Zhao
authors.affiliation: The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Henan Provincial People's Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
authors.forename: Ning Min
authors.initials: NM
authors.lastname: Zhao
authors.affiliation: Zhengzhou University, Zhengzhou City, Henan Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
mesh_terms: D000927:Anticonvulsants / Q000097:blood*; D002138:Calibration; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D016903:Drug Monitoring / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D006801:Humans; D000077213:Lamotrigine; D016014:Linear Models; D000078330:Oxcarbazepine; D011237:Predictive Value of Tests; D012015:Reference Standards; D015203:Reproducibility of Results; D013696:Temperature; D014227:Triazines / Q000097:blood*
chemical_list: D000927:Anticonvulsants; D014227:Triazines; D002220:Carbamazepine; D000077213:Lamotrigine; D000078330:Oxcarbazepine