title: In vitro evidence for endocrine-disrupting chemical (EDC)'s inhibition of drug metabolism.
abstract: BACKGROUND
  Humans can be frequently exposed to Bisphenol A (BPA) via multiple sources, and babies are considered to be the most sensitive group to exposure of BPA.
  
  
  OBJECTIVE
  To investigate the inhibition potential of BPA towards human liver microsomes (HLMs)-catalyzed zidovudine (AZT) glucuronidation.
  
  
  METHODS
  In vitro HLMs incubation system was used to investigate the inhibition potential of BPA towards AZT glucuronidation. Both Dixon and Lineweaver-Burk plots were employed to determine the inhibition kinetic type, and nonlinear repression was utilized to calculate the inhibition kinetic parameters (Ki).
  
  
  RESULTS
  Concentration-dependent inhibition of BPA towards AZT glucuronidation was observed. Both Dixon and Lineweaver-Burk plots showed that BPA exerted competitive inhibition towards the glucuronidation of AZT, and nonlinear repression with competitive equation was used to calculate the Ki value to be 3.2 µM.
  
  
  CONCLUSIONS
  Potential BPA-AZT interaction might occur when the patients administered with AZT is also exposed to BPA.
authors.affiliation: Gynecologic Surgical department, The General Hospital of Chinese People's Liberation Army, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Li
authors.affiliation: In patient department, The General Hospital of Chinese People's Liberation Army, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Gynecologic Surgical department, People's Hospital in Juye, Heze City, Shandong province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, The Military General Hospital of Beijing PLA, Beijing, 100700, China.
authors.forename: Ming-Lian
authors.initials: ML
authors.lastname: Yu
authors.affiliation: Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China.
authors.forename: Yan-Yang
authors.initials: YY
authors.lastname: Yu
authors.affiliation: Gynecologic Surgical department, The General Hospital of Chinese People's Liberation Army, China.
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Yao
mesh_terms: D000328:Adult; D001559:Benzhydryl Compounds / Q000493:pharmacokinetics*; D052244:Endocrine Disruptors / Q000493:pharmacokinetics*; D014453:Glucuronosyltransferase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010636:Phenols / Q000493:pharmacokinetics*; D015215:Zidovudine / Q000493:pharmacokinetics*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D014453:Glucuronosyltransferase; D010636:Phenols; D015215:Zidovudine; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A (BPA); human liver microsomes (HLMs); zidovudine (AZT)