title: Incubator proof miniaturized Holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections.
abstract: The in situ observation of cell movements and morphological parameters over longer periods of time under physiological conditions is critical in basic cell research and biomedical applications. The quantitative phase-contrast microscope applied in this study has a remarkably small size, therefore it can be placed directly into a humidified incubator. Here, we report on the successful application of this M4 Holomonitor to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications. A special mechanical stage was developed to position the sample into that range of the optical arrangement where digital autofocusing works with high reproducibility and precision. By in-depth analyzing the obtained single cell morphological parameters, we show that the limited vertical resolution of the optical setup results in underestimated single cell contact area and volume and overestimated single cell averaged thickness. We propose a simple model to correct the recorded data to obtain more precise single cell parameters. We compare the results with the kinetic data recorded by a surface sensitive optical biosensor, optical waveguide lightmode spectroscopy.
authors.affiliation: University of Pannonia, Doctoral School of Molecular and Nanotechnologies, 8200 Veszprém, HungarybNanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Peter
authors.affiliation: University of Pannonia, Doctoral School of Molecular and Nanotechnologies, 8200 Veszprém, HungarybNanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Nador
authors.affiliation: University of Pannonia, Doctoral School of Molecular and Nanotechnologies, 8200 Veszprém, HungarybNanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Juhasz
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Dobos
authors.affiliation: Enviroinvest Ltd., 7632 Pécs, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Körösi
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Inna
authors.initials: I
authors.lastname: Székács
authors.affiliation: University of Pannonia, Doctoral School of Molecular and Nanotechnologies, 8200 Veszprém, HungarybNanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Daniel
authors.initials: D
authors.lastname: Patko
authors.affiliation: University of Pannonia, Doctoral School of Molecular and Nanotechnologies, 8200 Veszprém, HungarybNanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary.
authors.forename: Robert
authors.initials: R
authors.lastname: Horvath
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D006367:HeLa Cells; D006696:Holography / Q000379:methods*; D006801:Humans; D051379:Mice; D008853:Microscopy / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism; D015203:Reproducibility of Results; D059010:Single-Cell Analysis / Q000379:methods; D013662:Tea / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry
chemical_list: D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D014025:Titanium