title: Effects of Chlorella sp. on nutrient treatment in cultures with different carbon to nitrogen ratios.
abstract: Chlorella sp. is often used in the treatment of wastewater to produce lipids, a practice which could go beyond wastewater treatment and be used to generate green energy. Our objectives here are to explore how the ratio of carbon to nitrogen (C/N) affects the removal of carbon and nitrogen in a wastewater treatment system, while simultaneously generating biomass and lipids. In this study, the C/N ratio is adjusted to 0.002, 6, 8, 10, 12 and 32. The results indicate that a C/N of 10 is sufficient to ensure the consumption of carbon and nitrogen, achieving the lowest concentration in the shortest culturing time (32 h). When nitrogen is lacking in the culture, there will be a slight decrease in the rate of carbon consumption which leads to a limitation of nitrogen and an increase in the lipid/cell density even at 96 h of culture time. The highest lipid content (0.57 g/L) and lipid increase rate (0.4 g/L) occurs with a C/N of 32. The greatest amount of biomass, 1.42 g/L is achieved when the C/N is 32. The carbon concentration is the main factor affecting the nitrogen consumption and the increase in the biomass and lipid content.
authors.affiliation: Department of Water Resources and Environmental Engineering, Tamkang University, 151, Ying-chung Road, Tamsui Dist., New Taipei City 251, Taiwan, R.O.C.
authors.forename: Hsin-Hui
authors.initials: HH
authors.lastname: Wu
authors.affiliation: Department of Water Resources and Environmental Engineering, Tamkang University, 151, Ying-chung Road, Tamsui Dist., New Taipei City 251, Taiwan, R.O.C.
authors.forename: Sue-Huai
authors.initials: SH
authors.lastname: Gau
authors.affiliation: Department of Water Resources and Environmental Engineering, Tamkang University, 151, Ying-chung Road, Tamsui Dist., New Taipei City 251, Taiwan, R.O.C.
authors.forename: Ming-Guo
authors.initials: MG
authors.lastname: Li
authors.affiliation: Department of Water Resources and Environmental Engineering, Tamkang University, 151, Ying-chung Road, Tamsui Dist., New Taipei City 251, Taiwan, R.O.C.
authors.forename: Yi-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Environmental Technology and Management, Taoyuan Innovation Institute of Technology, 414, Section 3, Chung-Shang East Road, Chung-Li, Taoyuan County 320, Taiwan, R.O.C. E-mail: sun.3409@hotmail.com.
authors.forename: Chang-Jung
authors.initials: CJ
authors.lastname: Sun
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D002708:Chlorella / Q000378:metabolism*; D055772:Green Chemistry Technology / Q000379:methods*; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D014874:Water Pollutants, Chemical; D002244:Carbon; D009584:Nitrogen