title: Structure of the neutral capsular polysaccharide of Acinetobacter baumannii NIPH146 that carries the KL37 capsule gene cluster.
abstract: Capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii NIPH146, and the following structure of branched pentasaccharide repeating unit was established by sugar analyses along with 1D and 2D NMR spectroscopy: In comparison to most other known capsular polysaccharides of A. baumannii, the CPS studied is neutral and lacks any specific monosaccharide component. The synthesis, assembly and export of this structure could be attributed to genes in a novel capsule biosynthesis gene cluster, designated KL37, which was found in the NIPH146 genome. The CPS of A. baumannii NIPH146 shares the α-d-Galp-(1→6)-β-d-Glcp-(1→3)-d-GalpNAc-(1→ trisaccharide fragment with the CPS units of several A. baumannii strains, including ATCC 17978 and LUH 5537 that carry the KL3 and KL22 gene clusters, respectively. KL37 contains two genes for glycosyltransferases that are related to two glycosyltransferase genes present in both KL3 and KL22, and the encoded proteins could be tentatively assigned to linkages between sugars in the CPS repeat.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Nikolay P
authors.initials: NP
authors.lastname: Arbatsky
authors.affiliation: M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Mikhail M
authors.initials: MM
authors.lastname: Shneider
authors.affiliation: School of Molecular Bioscience, The University of Sydney, Sydney, Australia; School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia.
authors.forename: Johanna J
authors.initials: JJ
authors.lastname: Kenyon
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors.forename: Anastasiya V
authors.initials: AV
authors.lastname: Popova
authors.affiliation: M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Miroshnikov
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Volozhantsev
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry* / Q000235:genetics*; D016667:Bacterial Capsules / Q000737:chemistry* / Q000235:genetics*; D002240:Carbohydrate Sequence; D056426:Genetic Loci / Q000235:genetics; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Acinetobacter baumannii; Capsular polysaccharide structure; Glycosyltransferase; K locus; KL37 gene cluster