title: Angiotensinogen gene M235T and angiotensin II-type 1 receptor gene A/C1166 polymorphisms in chronic obtructive pulmonary disease.
abstract: Chronic obstructive pulmonary disease (COPD) occurs irreversibly and is characterized by progressive airflow obstruction. Renin angiotensin system (RAS) has many different key enzymes and receptors that have a role for different systemic processes. We aimed to determine genotype and allele frequencies of angiotensinogen (AGT) M235T and angiotensin II-type 1 receptor (AT1-R) A/C1166 polymorphisms in patients with COPD. This study was performed on 56 unrelated COPD patients and 29 healthy subjects. DNA samples for each individual were isolated from peripheral blood by phenol/chloroform method, analyzed by polymerase chain reaction and enzymatic digestion methodologies. The distribution for each of AGT genotypes were 23.2% for MM (13), 75.0% for MT (42) and 1.8% for TT (1) in the COPD group; 37.9% for MM (11), 34.5% for MT (10) and 27.6% for TT (8) in the control group. The distribution of AGT genotypes was found significantly different between groups (X(2) = 18.604; df = 2; P = 0.000). The frequencies for each of the AT1-R genotypes were found as 53.6% for AA (30), 42.9% for AC (24), 3.6% for CC (2) in the COPD group; 55.2% for AA (16), 41.4% for AC (12) and 3.4% for CC (1) in the control group. The distribution of AT1-R genotypes did not change significantly between groups. Allele frequencies of interested genes were not significantly different between groups. We suggest that AGT polymorphism may play a role for the development of COPD. We believe these data can be served for large scale population genetics research, considering the frequency of AGT and AT1-R genes and alleles in COPD patients in the Turkish population.
authors.affiliation: Departments of Physiology, Dumlupınar University, Medical Faculty Kütahya 43100, Turkey.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Ayada
authors.affiliation: Departments of Chest Diseases, Dumlupınar University, Medical Faculty Kütahya 43100, Turkey.
authors.forename: Ümran
authors.initials: Ü
authors.lastname: Toru
authors.affiliation: Departments of Physiology, Dumlupınar University, Medical Faculty Kütahya 43100, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Genç
authors.affiliation: Departments of Medical Biology, Dumlupınar University, Medical Faculty Kütahya 43100, Turkey.
authors.forename: Server
authors.initials: S
authors.lastname: Şahin
authors.affiliation: Department of Physiology, Pamukkale University, Medical Faculty Denizli 20070, Turkey.
authors.forename: Sebahat
authors.initials: S
authors.lastname: Turgut
authors.affiliation: Department of Physiology, Pamukkale University, Medical Faculty Denizli 20070, Turkey.
authors.forename: Günfer
authors.initials: G
authors.lastname: Turgut
keywords: A/C1166 polymorphism; Angiotensinogen; M235T polymorphism; angiotensin-II type 1 receptor; chronic obstructive pulmonary disease