title: Effect of Ethanol Accumulation on Porcine Interferon-α Production by Pichia pastoris and Activities of Key Enzymes in Carbon Metabolism.
abstract: In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high ethanol concentration (>10 g/L) on pIFN-α production, and activities of key enzymes in carbon metabolism were discussed. As a result, compared with control group, pIFN-α expression level was decreased about 4~12 folds under long-term high ethanol concentration, from the level above 3 g/L to the level under 1 g/L; pIFN-α expression level was decreased about 8 folds under instantaneous high ethanol concentration, reaching to the low level of 0.42 g/L. The low production of pIFN-α was caused by the severe inhibitory effect of ethanol on these enzymes.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China, dingjian@jiangnan.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Ding
authors.forename: Minjie
authors.initials: M
authors.lastname: Gao
authors.forename: Guoli
authors.initials: G
authors.lastname: Hou
authors.forename: Kexue
authors.initials: K
authors.lastname: Liang
mesh_terms: D000818:Animals; D002244:Carbon / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods*; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D013552:Swine; D013997:Time Factors
chemical_list: D016898:Interferon-alpha; D000431:Ethanol; D002244:Carbon