title: Protection by LPS-induced inhibitory CD11b(+) cells on corneal allograft.
abstract: OBJECTIVE
  It is widely reported that CD11b(+)Gr1+ myeloid-derived suppressor cells can cause allograft tolerance in mice and human, however, little is known on the therapy role in chronic transplantation rejection. In this paper, their role in corneal transplantation was studied for the first time.
  
  
  METHODS
  Inhibitory CD11b(+) cells were obtained by murine LPS-induced septic model. Phenotype, endocytosis, antigen presenting ability, and T cell suppression assays were performed by flow cytometry analysis. The suppressive ability in vivo was analyzed by targeting allogeneic corneal transplantation.
  
  
  RESULTS
  LPS was intraperitoneally injected into C57BL/6 mice, the percentage of CD11b(+) Gr1+ cells was increased in mice spleen, blood, and bone marrow, respectively. Compared with control mice, Ly6C, TLR2, and MHC-11 expression were higher in LPS treated mice. CD11b(+) Gr1+ cells could inhibit allogenic corneal reaction in vivo after adoptive transfer, in consistent with an observation of inhibition effect on the antigen presenting cells (APCs) and CD4+ T cells proliferation in vitro.
  
  
  CONCLUSIONS
  CD11b(+) cells induced by LPS could function as inhibitory APCs, suppress CD4+ T cells proliferation and improve corneal allograft survival. Predictly, its application for cells transfer therapy in clinic in the further.
authors.affiliation: Department of Corneal and Refractive Disease, Tianjin Medical University Eye Hospital Tianjin 300384, P. R. China ; Department of Corneal Disease, Shanxi Eye Hospital Taiyuan 030002, Shanxi, P. R. China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Corneal and Refractive Disease, Tianjin Medical University Eye Hospital Tianjin 300384, P. R. China.
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Zhao
keywords: CD11b+; LPS; corneal transplantation