title: [The expression of retrogene Nanogp8 is related to the biological characteristics of human SGC-7901 gastric cancer cells].
abstract: OBJECTIVE
  To explore the effects of NANOGP8 on proliferation, apoptosis, cell cycle, invasion and migration of human SGC-7901 gastric cancer cells.
  
  
  METHODS
  Eukaryotic expression vector pEGFP-N1-NANOGP8 and recombinant plasmid shRNA-NANOGP8 were constructed and confirmed by enzyme digestion and sequencing analysis. Then, vector pEGFP-N1-NANOGP8 and recombinant plasmid shRNA-NANOGP8 were transfected into SGC-7901 cells via liposome. The expression of NANOGP8 mRNA and protein were tested by fluorescence microscopy, reverse transcription PCR and Western blotting, respectively. The proliferation of SGC-7901 cells was detected by CCK-8 assay. The apoptosis and cell cycle were examined by flow cytometry. TranswellTM assay proved the changes in the invasion and migration abilities of SGC-7901 cells.
  
  
  RESULTS
  The recombinant plasmids pEGEP-N1-NANOGP8 and pshRNA-NANOGP8 were constructed successfully and transfected into gastric cancer cells. pEGEP-N1-NANOGP8 transfection group showed high expression of NANOGP8, conversely, pshRNA-NANOGP8 transfection group showed low expression of NANOGP8. High expression of NANOGP8 significantly promoted the proliferation of tumor cells, arrested cells cycle in the S phase, decreased apoptotic cells and increased cell invasion and migration obviously. While the low expression group presented with inhibited cell proliferation, cell-cycle arrest in the G0/G1 phase, promoted cell apoptosis and inhibited migration and invasion.
  
  
  CONCLUSIONS
  The transcription and expression of retrogene NANOGP8 has a close relationship with the proliferation, cycle, apoptosis, migration and invasion of SGC-7901 gastric cancer cells.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D000071317:Nanog Homeobox Protein; D009361:Neoplasm Invasiveness; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D012333:RNA, Messenger