title: Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila's Whole Larvae and Tissues.
abstract: The identification and study of protein's function in several model organisms is carried out using both nuclear and cytoplasmic extracts. For a long time, Drosophila's embryos have represented the main source for protein extractions, although in the last year, the importance of collecting proteins extracts also from larval tissues has also been understood. Here we report a very simple protocol, improved by a previously developed method, to produce in a single extraction both highly stable nuclear and cytoplasmic protein extracts from a small quantity of whole Drosophila's larvae or tissues, suitable for biochemical analyses like co-immunoprecipitation.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Viale delle Scienze, Universita' degli Studi di Palermo, Palermo 90128, Italy. lucalopiccolo@gmail.com.
authors.forename: Luca
authors.initials: L
authors.lastname: Lo Piccolo
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Viale delle Scienze, Universita' degli Studi di Palermo, Palermo 90128, Italy. rosabonaccorsog@gmail.com.
authors.forename: Rosa
authors.initials: R
authors.lastname: Bonaccorso
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Viale delle Scienze, Universita' degli Studi di Palermo, Palermo 90128, Italy. mconorati@dti.telethon.it.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Onorati
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D029721:Drosophila Proteins / Q000302:isolation & purification*; D004331:Drosophila melanogaster / Q000737:chemistry*; D007814:Larva / Q000737:chemistry
chemical_list: D029721:Drosophila Proteins
keywords: Drosophila; hnRNPs; proteins extraction