title: The triglyceride-lowering effect of supplementation with dual probiotic strains, Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032: Reduction of fasting plasma lysophosphatidylcholines in nondiabetic and hypertriglyceridemic subjects.
abstract: OBJECTIVE
  This study evaluated the triglyceride (TG)-lowering effects of consuming dual probiotic strains of Lactobacillus curvatus (L. curvatus) HY7601 and Lactobacillus plantarum (L. plantarum) KY1032 on the fasting plasma metabolome.
  
  
  RESULTS
  A randomized, double-blind, placebo-controlled study was conducted on 92 participants with hypertriglyceridemia but without diabetes. Over a 12-week testing period, the probiotic group consumed 2 g of powder containing 5 × 10(9) colony-forming units (cfu) of L. curvatus HY7601 and 5 × 10(9) cfu of L. plantarum KY1032 each day, whereas the placebo group consumed the same product without probiotics. Fasting plasma metabolomes were profiled using UPLC-LTQ-Orbitrap MS. After 12 weeks of treatment, the probiotic group displayed a 20% reduction (p = 0.001) in serum TGs and 25% increases (p=0.001) in apolipoprotein A-V (apoA-V). At the 12-week follow-up assessment, the following 11 plasma metabolites were significantly reduced in the probiotic group than the placebo group: palmitoleamide, palmitic amide, oleamide, and lysophosphatidyl choline (lysoPC) containing C14:0, C16:1, C16:0, C17:0, C18:3, C18:2, C18:1, and C20:3. In the probiotic group, changes (▵) in TG were negatively correlated with ▵ apoA-V, which was positively correlated with ▵ FFA. In addition, ▵ FFA was strongly and positively correlated with ▵ lysoPCs in the probiotic group but not the placebo group.
  
  
  CONCLUSIONS
  The triglyceride-lowering effects of probiotic supplementation, partly through elevated apoA-V, in borderline to moderate hypertriglyceridemic subjects showed reductions in plasma metabolites, fatty acid primary amides and lysoPCs (NCT02215694; http://www.clinicaltrials.gov). Clinical trials: NCT02215694; http://www.clinicaltrials.gov.
authors.affiliation: Interdisciplinary Course of Science for Aging, Yonsei University, Seoul, South Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Ahn
authors.affiliation: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, South Korea.
authors.forename: M
authors.initials: M
authors.lastname: Kim
authors.affiliation: Korea Yakult Co., Ltd., Yongin, Gyeonggi, South Korea.
authors.forename: Y-T
authors.initials: YT
authors.lastname: Ahn
authors.affiliation: Korea Yakult Co., Ltd., Yongin, Gyeonggi, South Korea.
authors.forename: J-H
authors.initials: JH
authors.lastname: Sim
authors.affiliation: Korea Yakult Co., Ltd., Yongin, Gyeonggi, South Korea.
authors.forename: I-D
authors.initials: ID
authors.lastname: Choi
authors.affiliation: Department of Family Practice, National Health Insurance Corporation Ilsan Hospital, Goyang, South Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, South Korea; National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, South Korea; Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, South Korea. Electronic address: jhleeb@yonsei.ac.kr.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000097:blood; D019587:Dietary Supplements; D004311:Double-Blind Method; D005215:Fasting / Q000097:blood; D005227:Fatty Acids / Q000097:blood; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood* / Q000178:diet therapy*; D048191:Lactiplantibacillus plantarum / Q000145:classification*; D008244:Lysophosphatidylcholines / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D005227:Fatty Acids; D008244:Lysophosphatidylcholines; D014280:Triglycerides
keywords: Apolipoprotein A-V; Hypertriglyceridemic; Lysopcs; Probiotic; Triglyceride