title: Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome.
abstract: The present study aimed to evaluate the expression of CD80 and CD18 in subpopulations of peripheral blood leukocytes and oxidative kidney damage in rats with nephrotic syndrome (NS) induced by doxorubicin (Dox) in comparison to control animals at different time points. Male adult Wistar rats were submitted to 24-hour urine and blood collection for biochemical and immunological analysis at 7, 14, 21, and 28 days after Dox injection. After euthanasia, the kidneys were removed for histological analysis and the evaluation of oxidative stress. The phenotypic characterization of leukocytes was performed using flow cytometry. Dox-injected animals exhibited increased CD18 expression in cytotoxic T lymphocytes, NK cells, and monocytes and high CD80 expression in monocytes. Kidney oxidative damage was positively correlated with CD80 expression in monocytes and serum levels of creatinine. These results suggest that phagocytic and cytotoxic cells are preferentially recruited to the tissue injury site, which may contribute to kidney dysfunction in this animal model of NS. The blockade of integrin and costimulatory molecules may provide new therapeutic opportunities for NS.
authors.affiliation: Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, Brazil.
authors.forename: Wagner de Fátima
authors.initials: Wde F
authors.lastname: Pereira
authors.affiliation: Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, Brazil.
authors.forename: Gustavo Eustáquio Alvim
authors.initials: GE
authors.lastname: Brito-Melo
authors.affiliation: Immunopathology Laboratory, Center for Research in Biological Sciences (LIMP/NUPEB), Universidade Federal de Ouro Preto (UFOP), 30140-081 Ouro Preto, MG, Brazil.
authors.forename: Cláudia Martins
authors.initials: CM
authors.lastname: Carneiro
authors.affiliation: Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, Brazil.
authors.forename: Dirceu de Sousa
authors.initials: Dde S
authors.lastname: Melo
authors.affiliation: Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, Brazil.
authors.forename: Karine Beatriz
authors.initials: KB
authors.lastname: Costa
authors.affiliation: Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, Brazil.
authors.forename: Fábio Lourenço Tadeu
authors.initials: FL
authors.lastname: Guimarães
authors.affiliation: Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, Brazil.
authors.forename: Etel
authors.initials: E
authors.lastname: Rocha-Vieira
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, Pediatric Nephrology Unit, Universidade Federal de Minas Gerais (UFMG), 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Érica Leandro Marciano
authors.initials: ÉL
authors.lastname: Vieira
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, Pediatric Nephrology Unit, Universidade Federal de Minas Gerais (UFMG), 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Simões e Silva
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D005434:Flow Cytometry; D007668:Kidney / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009404:Nephrotic Syndrome / Q000276:immunology* / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D004317:Doxorubicin