title: Artemisia asiatica Nakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways.
abstract: The present study aimed to examine the anti-inflammatory effects and potential mechanism of action of Artemisia asiatica Nakai (A. asiatica Nakai) extract in activated murine macrophages. A. asiatica Nakai extract showed dose-dependent suppression of lipopolysaccharide (LPS)-induced nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 activity. It also showed dose-dependent inhibition of nuclear factor-κB (NF-κB) translocation from the cytosol to the nucleus and as an inhibitor of NF-κB-alpha phosphorylation. The extract's inhibitory effects were found to be mediated through NF-κB inhibition and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 in LPS-stimulated J774A.1 murine macrophages, suggesting a potential mechanism for the anti-inflammatory activity of A. asiatica Nakai. To our knowledge, this is the first report of the anti-inflammatory effects of A. asiatica Nakai on J774A.1 murine macrophages; these results may help develop functional foods possessing an anti-inflammatory activity.
authors.affiliation: 1 Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University , Chungju, Korea.|2 Korea Nokyong Research Center, Konkuk University , Chungju, Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.affiliation: 1 Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University , Chungju, Korea.|2 Korea Nokyong Research Center, Konkuk University , Chungju, Korea.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Tang
authors.affiliation: 3 Division of Sport Science, College of Science and Technology, Konkuk University , Chungju, Korea.
authors.forename: Kwang-Suk
authors.initials: KS
authors.lastname: Cha
authors.affiliation: 3 Division of Sport Science, College of Science and Technology, Konkuk University , Chungju, Korea.
authors.forename: Heeri
authors.initials: H
authors.lastname: Choi
authors.affiliation: 4 Department of Korean Food and Culinary Arts, Youngsan University , Busan, Korea.
authors.forename: Chun Bok
authors.initials: CB
authors.lastname: Lee
authors.affiliation: 5 Institute for Natural Science Research, Hannam University , Daejeon, Korea.
authors.forename: Jin-Hwan
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: 6 Division of Sports Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Kim
authors.affiliation: 7 Department of Sports Industry & Welfare, Wonkwang University , Jeonbuk, Korea.
authors.forename: Jong-Shik
authors.initials: JS
authors.lastname: Kim
authors.affiliation: 8 Department of Physical Medicine and Rehabilitation, Konkuk University Chungju Hospital , Chungbuk, Korea.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Kim
authors.affiliation: 9 Department Pathology, Sanbon Medical Center, Wonkwang University , Sanbon, Korea.
authors.forename: Weon Cheol
authors.initials: WC
authors.lastname: Han
authors.affiliation: 10 Department of Medical Non-Commissioned Officer, Wonkwang Health Science University , Jeonbuk, Korea.
authors.forename: Suck-Jun
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: 11 Department of Industrial Engineering, Seoul National University , Seoul, Korea.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Lee
authors.affiliation: 3 Division of Sport Science, College of Science and Technology, Konkuk University , Chungju, Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: 12 Laboratory of Immunotoxicology, Department of Pharmacology, School of Medicine, Kyungpook National University , Daegu, Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D019306:Artemisia / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects* / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Artemisia asiatica Nakai; ERK1/2; NF-κB; p38