title: Studies on metabolism of total glucosides of paeony from Paeoniae Radix Alba in rats by UPLC-Q-TOF-MS/MS.
abstract: Total glucosides of paeony are the active constituents of Paeoniae Radix Alba. In this study, a novel strategy was proposed to find more metabolites and the differences between paeoniflorin, albiflorin and total glucosides of paeony (TGP). This strategy was characterized as follows: firstly, the animals were divided into three groups (paeoniflorin, albiflorin and TGP) to identify the source of TGP metabolites from paeoniflorin or albiflorin; secondly, a generic information-dependent acquisition scan for the low-level metabolites was triggered by the multiple mass defect filter and dynamic background subtraction; thirdly, the metabolites were identified with a combination of data-processing methods including mass defect filtering, neutral loss filtering and product ion filtering; finally, a comparative study was used in the metabolism of paeoniflorin, albiflorin and TGP. Based on the strategy, 18 metabolites of TGP, 10 metabolites of paeoniflorin and 13 metabolites of albiflorin were identified respectively. The results indicated that the hydrolysis, conjugation reaction and oxidization were the major metabolic pathways, and the metabolic sites were the glycosidic linkage, the ester bond and the benzene ring. This study is first to explore the metabolism of TGP, and these findings enhance our understanding of the metabolism and the interactions of paeoniflrin and albiflorin in TGP.
authors.affiliation: Pharmacology Laboratory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Cao
authors.affiliation: Pharmacology Laboratory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Pharmacology Laboratory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.|Shijiazhuang City Hospital of Traditional Chinese Medicine, Shijiazhuang, 050091, China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Li
authors.affiliation: Pharmacology Laboratory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, 050035, China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Fan
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, 050035, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, 050035, China.
authors.forename: Minyan
authors.initials: M
authors.lastname: Liu
authors.affiliation: Pharmacology Laboratory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Niu
mesh_terms: D000818:Animals; D001952:Bridged-Ring Compounds / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D005960:Glucosides / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D008297:Male; D039821:Monoterpenes / Q000378:metabolism*; D029596:Paeonia / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001952:Bridged-Ring Compounds; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; C015423:peoniflorin; C014959:albiflorin
keywords: UPLC-Q-TOF-MS/MS; albiflorin; identification; paeoniflorin; total glucosides of paeony