title: Effect of a carbonated HAP/β-glucan composite bone substitute on healing of drilled bone voids in the proximal tibial metaphysis of rabbits.
abstract: A novel elastic hydroxyapatite-based composite of high surgical handiness has been developed. Its potential application in orthopedics as a filler of bone defects has been studied. The biomaterial was composed of carbonated hydroxyapatite (CHAP) granules and polysaccharide polymer (β-1,3-glucan). Cylinders of 4mm in diameter and 6mm in length were implanted into bone cavities created in the proximal metaphysis of tibiae of 24 New Zealand white rabbits. 18 sham-operated animals were used as controls. After 1, 3 or 6 months, the rabbits were euthanized, the bones were harvested and subjected to analysis. Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. Peripheral quantitative computed tomography (pQCT) indicated the stimulating effect of the biomaterial on bone formation and mineralization. Densitometry (DXA) analysis suggested that biomineralization of bones was preceded by bioresorption and gradual disappearance of porous ceramic granules. The findings suggest that the CHAP-glucan composite material enables regeneration of bone tissue and could serve as a bone defect filler.
authors.affiliation: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland. Electronic address: leszek.borkowski@umlub.pl.
authors.forename: Leszek
authors.initials: L
authors.lastname: Borkowski
authors.affiliation: Department of Animal Physiology, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pawłowska
authors.affiliation: Department of Animal Physiology, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
authors.forename: Radosław P
authors.initials: RP
authors.lastname: Radzki
authors.affiliation: Department of Animal Physiology, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Bieńko
authors.affiliation: Department and Clinic of Animal Surgery, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Polkowska
authors.affiliation: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Belcarz
authors.affiliation: Department of Companion and Wildlife Animals, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Karpiński
authors.affiliation: Independent Radiology Unit at Lublin Small Animals Medical Centre, Stefczyka 11, 20-151 Lublin, Poland.
authors.forename: Tymoteusz
authors.initials: T
authors.lastname: Słowik
authors.affiliation: Children's Orthopaedic Clinic and Rehabilitation Department, Medical University of Lublin, Chodzki 2, 20-093 Lublin, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Matuszewski
authors.affiliation: Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ślósarczyk
authors.affiliation: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Ginalska
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D013977:Tibia / Q000187:drug effects* / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D047071:beta-Glucans; C033363:beta-1,3-glucan; D017886:Durapatite
keywords: Bone; Composite; Implantation; Mineralization; Osseointegration; Rabbit