title: Sensitive simultaneous determination of three sulfanilamide artificial sweeters by capillary electrophoresis with on-line preconcentration and contactless conductivity detection.
abstract: A sensitive method followed by capillary electrophoresis with on-line perconcentration and capacitively coupled contactless conductivity detection (CE-C(4)D) was evaluated as a novel approach for the determination of three sulfanilamide artificial sweeteners (acesulfame-K, sodium saccharin and sodium cyclamate) in beverages. The on-line preconcentration technique, namely field-amplified sample injection, coupled with CE-C(4)D were successfully developed and optimized. The separation was achieved within 10 min under the following conditions: an uncoated fused-silica capillary (45 cm × 50 μm i.d., Leff=40 cm), 20 mmol L(-1) HAc as running buffer, separation voltage of -12 kV, electrokinetic injection of -11 kV × 8 s. The detection limits of acesulfame-K, sodium saccharin and sodium cyclamate were 4.4, 6.7 and 8.8 μg L(-1), respectively. The relative standard deviation varied in the range of 3.0-5.0%. Results of this study show a great potential method for the fast screening of these artificial sweeteners contents in commercial beverages.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: ShengJi
authors.initials: S
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Yuezhou
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: cesxty@mail.sysu.edu.cn.
authors.forename: Tianyao
authors.initials: T
authors.lastname: Xie
mesh_terms: D001628:Beverages / Q000032:analysis* / Q000592:standards; D003494:Cyclamates / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D015203:Reproducibility of Results; D012439:Saccharin / Q000032:analysis*; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000032:analysis*; D013549:Sweetening Agents / Q000032:analysis*
chemical_list: D003494:Cyclamates; D013424:Sulfanilamides; D013549:Sweetening Agents; D000077145:Sulfanilamide; D012439:Saccharin
keywords: Acesulfame-K; Capillary electrophoresis; Contactless conductivity detection; On-line preconcentration; Sodium cyclamate; Sodium saccharin