title: Gab2 is a novel prognostic factor for colorectal cancer patients.
abstract: Gab2 (Grb2-associated binder 2), a member of the DOS/Gab family of scaffolding adapters, serves as a critical signal amplifier downstream of various growth factor receptors. Recent studies have identified that Gab2 is overexpressed in several cancer types and that increased Gab2 expression promotes cell proliferation, cell transformation, and tumor progression. Here, we show for the first time that Gab2 protein is overexpressed in clinical colorectal cancer (CRC) specimens. Elevated mRNA (P=0.014) expression and protein (P=0.003) expression of Gab2 were found in most CRC tissues compared with the matched adjacent non-tumor tissues using real-time quantitative reverse transcription PCR (qRT-PCR) and western blotting, respectively. Immunohistochemical analyses showed that Gab2 protein was upregulated in CRC tissues relative to adjacent normal tissues (P<0.001), and this overexpression was significantly correlated with lymph node metastasis (P=0.007), distant metastasis (P<0.001) and TNM stage (P=0.002). According to Kaplan-Meier model, CRC patients with Gab2-positive had a significantly poorer prognosis compared to those with Gab2-negative (P=0.007). Multivariate analysis suggested that the positive expression of Gab2 protein was an independent prognostic factor for CRC patients. In conclusion, our data demonstrated that Gab2 expression may play an important role in the progression of CRC, and underscored that Gab2 has the potential value as a prognostic predictor for CRC patients.
authors.affiliation: Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province Zunyi, China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province Zunyi, China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province Zunyi, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province Zunyi, China.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province Zunyi, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province Zunyi, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Oncology, Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Feng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis* / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D015153:Blotting, Western; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C490895:GAB2 protein, human; D012333:RNA, Messenger
keywords: Gab2; colorectal cancer; immunohistochemistry; prognosis; qRT-PCR; western blotting