title: Toxicity of 8-Hydroxyquinoline in Cryprinus carpio Using the Acute Toxicity Test, Hepatase Activity Analysis and the Comet Assay.
abstract: To evaluate the environmental toxicity of 8-hydroxyquinoline (8-HOQ), an important industrial raw material found in China's major ornamental fish, Cryprinus carpio, using the acute toxicity test, hepatase activity analysis and the comet assay. The results indicated that 8-HOQ had significant acute toxicity in adult C. carpio with a 96 h-LC50 of 1.15 and 0.22 mg L(-1) hepatic quinoline residues as assessed by HPLC. 8-HOQ also induced genotoxicity in the form of strand breaks in the DNA of hepatic cells as shown by the comet assay. With regard to physiological toxicity, 8-HOQ induced a decrease in the activities of hepatic GOT and GPT with increased exposure concentration and time. These data suggest that 8-HOQ may be toxic to the health of aquatic organisms when accidentally released into aquatic ecosystems. The data also suggest that the comet assay may be used in biomonitoring to determine 8-HOQ genotoxicity and hepatic GPT and GOT activities may be potential biomarkers of physiological toxicity.
authors.affiliation: College of Life Science, Henan Normal University, No. 46, East Jianshe Road, Xinxiang City, 453007, Henan, People's Republic of China, yanshuaiguo@163.com.
authors.forename: Shuaiguo
authors.initials: S
authors.lastname: Yan
authors.forename: Lili
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Dou
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Du
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: He
authors.forename: Mingge
authors.initials: M
authors.lastname: Nan
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Chang
authors.forename: Ping
authors.initials: P
authors.lastname: Nan
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism*; D002681:China; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods; D015125:Oxyquinoline / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010575:Pesticides; D014874:Water Pollutants, Chemical; D015125:Oxyquinoline