title: Atlantic Salmon Carries a Range of Novel O-Glycan Structures Differentially Localized on Skin and Intestinal Mucins.
abstract: Aquaculture is a growing industry, increasing the need for understanding host-pathogen interactions in fish. The skin and mucosal surfaces, covered by a mucus layer composed of mucins, is the first point of contact between fish and pathogens. Highly O-glycosylated mucins have been shown to be an important part of the defense against pathogens, and pathogens bind to host surfaces using lectin-like adhesins. However, knowledge of piscine O-glycosylation is very limited. We characterized mucin O-glycosylation of five freshwater acclimated Atlantic salmon, using mass spectrometry. Of the 109 O-glycans found, most were sialylated and differed in distribution among skin, pyloric ceca, and proximal and distal intestine. Skin O-glycans were shorter (2-6 residues) and less diverse (33 structures) than intestinal O-glycans (2-13 residues, 93 structures). Skin mucins carried O-glycan cores 1, 2, 3, and 5 and three types of sialic acids (Neu5Ac, Neu5Gc, and Kdn) and had sialyl-Tn as the predominant structure. Intestinal mucins carried only cores 1, 2, and 5, Neu5Ac was the only sialic acid present, and sialylated core 5 was the most dominant structure. This structural characterization can be used for identifying structures of putative importance in host-pathogen interactions for further testing in biological assays and disease intervention therapies.
authors.affiliation: †Department of Medical Chemistry and Cell Biology, ‡Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg SE-405 30, Sweden.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Jin
authors.affiliation: †Department of Medical Chemistry and Cell Biology, ‡Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg SE-405 30, Sweden.
authors.forename: János Tamás
authors.initials: JT
authors.lastname: Padra
authors.affiliation: †Department of Medical Chemistry and Cell Biology, ‡Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg SE-405 30, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Sundell
authors.affiliation: †Department of Medical Chemistry and Cell Biology, ‡Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg SE-405 30, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Sundh
authors.affiliation: †Department of Medical Chemistry and Cell Biology, ‡Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg SE-405 30, Sweden.
authors.forename: Niclas G
authors.initials: NG
authors.lastname: Karlsson
authors.affiliation: †Department of Medical Chemistry and Cell Biology, ‡Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg SE-405 30, Sweden.
authors.forename: Sara K
authors.initials: SK
authors.lastname: Lindén
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid; D029941:Fish Proteins / Q000378:metabolism*; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism*; D054794:Glycomics / Q000379:methods; D006031:Glycosylation; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D009928:Organ Specificity; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D020317:Salmo salar / Q000378:metabolism*; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D029941:Fish Proteins; D009077:Mucins; D011134:Polysaccharides; D012794:Sialic Acids; D005643:Fucose; D009439:Neuraminidase
keywords: Atlantic salmon; O-glycan; fish; gastrointestinal tract; glycosylation; mucins; mucus; skin