title: Deciphering the protein-RNA recognition code: combining large-scale quantitative methods with structural biology.
abstract: RNA binding proteins (RBPs) are key factors for the regulation of gene expression by binding to cis elements, i.e. short sequence motifs in RNAs. Recent studies demonstrate that cooperative binding of multiple RBPs is important for the sequence-specific recognition of RNA and thereby enables the regulation of diverse biological activities by a limited set of RBPs. Cross-linking immuno-precipitation (CLIP) and other recently developed high-throughput methods provide comprehensive, genome-wide maps of protein-RNA interactions in the cell. Structural biology gives detailed insights into molecular mechanisms and principles of RNA recognition by RBPs, but has so far focused on single RNA binding proteins and often on single RNA binding domains. The combination of high-throughput methods and detailed structural biology studies is expected to greatly advance our understanding of the code for protein-RNA recognition in gene regulation, as we review in this article.
authors.affiliation: Institute of Structural Biology, Helmholtz Zentrum M, ü, nchen, München, Germany.|Department Chemie, Center for Integrated Protein Science Munich at Biomolecular NMR Spectroscopy, Technische Universität München, Garching, Germany.
authors.forename: Janosch
authors.initials: J
authors.lastname: Hennig
authors.affiliation: Institute of Structural Biology, Helmholtz Zentrum M, ü, nchen, München, Germany.|Department Chemie, Center for Integrated Protein Science Munich at Biomolecular NMR Spectroscopy, Technische Universität München, Garching, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Sattler
mesh_terms: D000818:Animals; D001665:Binding Sites; D019295:Computational Biology; D006801:Humans; D064267:Intrinsically Disordered Proteins / Q000737:chemistry / Q000502:physiology; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000502:physiology
chemical_list: D064267:Intrinsically Disordered Proteins; D016601:RNA-Binding Proteins; D012313:RNA
keywords: CLIP; RNA binding proteins; RNA recognition motif; cooperativity; mRNA interactome; protein-RNA recognition; structural biology