title: Granulocyte/macrophage colony-stimulating factor attenuates endothelial hyperpermeability after thermal injury.
abstract: Microvascular hyperpermeability followed by burn injury is the main cause of shock, and cardiovascular collapse can result if the condition is treated improperly. Our previous studies demonstrated that granulocyte/macrophage colony-stimulating factor (GM-CSF) clearly reduces microvascular permeability and protects microvessels against burn injury. However, the mechanism underlying the protective function of GM-CSF on burn-injured microvessels remains unknown. This study aimed to investigate the effect and mechanism of GM-CSF on endothelial cells after exposure to burn serum. We demonstrated that GM-CSF reduced post-burn endothelial "capillary leak" by inhibiting the activity of RhoA and maintaining the membrane localization of VE-cadherin. Membranous VE-cadherin enhances adherens junctions between endothelial cells and co-localizes with and activates VEGFR2, which protect cells from burn serum-induced apoptosis. Our findings suggest that the protective mechanism of GM-CSF on burn serum-injured endothelial monolayer hyperpermeability is achieved by strengthening cell adherens junctions and improving cell viability.
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Julin
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Burns, The First Affiliated Hospital of Sun Yat-sen University China.
authors.forename: Shaohai
authors.initials: S
authors.lastname: Qi
keywords: Granulocyte/macrophage colony-stimulating factor; RhoA; burn; permeability