title: miR-215 overexpression distinguishes ampullary carcinomas from pancreatic carcinomas.
abstract: Distinguishing ampullary carcinoma from pancreatic carcinoma is important because of their different prognoses. microRNAs are differentially expressed according to the tissue of origin. However, there is rare research on the differential diagnosis between the two types of cancers by microRNA in periampullary cancers. The present study was undertaken to compare microRNA profiles between ampullary and pancreatic carcinomas using microarrays. miR-215 was most significantly overexpressed in ampullary carcinomas; whereas the expressions of miR-134 and miR-214 were significantly lower in ampullary carcinomas than in pancreatic carcinomas. When these discriminatory microRNAs were applied to liver metastases, they were correctly predicted for the tissue of origin. Although this study is limited by small sample size, striking difference in microRNA expression and concordant expression of discriminating microRNAs in primary tumors and metastases suggest that these novel discriminatory microRNAs warrant future validation.
authors.affiliation: Biomolecular Function Research Branch, National Cancer Center, 323 Ilsan-ro, Goyang, Gyeonggi, 410-769, Korea. hkim@ncc.re.kr.
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Choi
authors.forename: Sang Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Hark Kyun
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000368:Aged; D014670:Ampulla of Vater / Q000737:chemistry* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics* / Q000556:secondary; D003138:Common Bile Duct Neoplasms / Q000235:genetics* / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011237:Predictive Value of Tests; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C569273:MIRN134 microRNA, human; C547397:MIRN214 microRNA, human; C553667:MIRN215 microRNA, human; D035683:MicroRNAs