title: Structure and Mechanism of the Siderophore-Interacting Protein from the Fuscachelin Gene Cluster of Thermobifida fusca.
abstract: Microbial iron acquisition is a complex process and frequently a key and necessary step for survival. Among the several paths for iron assimilation, small molecule siderophore-mediated transport is a commonly employed strategy of many microorganisms. The chemistry and biology of the extraordinary tight and specific binding of siderophores to metal is also exploited in therapeutic treatments for microbial virulence and metal toxicity. The intracellular fate of iron acquired via the siderophore pathway is one of the least understood steps in the complex process at the molecular level. A common route to cellular incorporation is the single-electron reduction of ferric to ferrous iron catalyzed by specific and/or nonspecific reducing agents. The biosynthetic gene clusters for siderophores often contain representatives of one or two families of redox-active enzymes: the flavin-containing "siderophore-interacting protein" and iron-sulfur ferric siderophore reductases. Here we present the structure and characterization of the siderophore-interacting protein, FscN, from the fuscachelin siderophore gene cluster of Thermobifida fusca. The structure shows a flavoreductase fold with a noncovalently bound FAD cofactor along with an unexpected metal bound adjacent to the flavin site. We demonstrated that FscN is redox-active and measured the binding and reduction of ferric fuscachelin. This work provides a structural basis for the activity of a siderophore-interacting protein and further insight into the complex and important process of iron acquisition and utilization.
authors.affiliation: Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, Florida 32611, United States.
authors.forename: Kunhua
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, Florida 32611, United States.
authors.forename: Wei-Hung
authors.initials: WH
authors.lastname: Chen
authors.affiliation: Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, Florida 32611, United States.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Bruner
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding; D016415:Sequence Alignment; D017262:Siderophores / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017262:Siderophores