title: Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays.
abstract: Lotus (Nelumbo nucifera) leaves, a traditional Chinese medicinal herb, are rich in flavonoids. In an effort to thoroughly analyze their flavonoid components, macroporous resin chromatography coupled with HPLC-MS/MS was employed to simultaneously enrich and identify flavonoids from lotus leaves. Flavonoids extracted from lotus leaves were selectively enriched in the macroporous resin column, eluted subsequently as fraction II, and successively subjected to analysis with the HPLC-MS/MS and bioactivity assays. Altogether, fourteen flavonoids were identified, four of which were identified from lotus leaves for the first time, including quercetin 3-O-rhamnopyranosyl-(1→2)-glucopyranoside, quercetin 3-O-arabinoside, diosmetin 7-O-hexose, and isorhamnetin 3-O-arabino- pyranosyl-(1→2)-glucopyranoside. Further bioactivity assays revealed that these flavonoids from lotus leaves possess strong antioxidant activity, and demonstrate very good potential to be explored as food supplements or even pharmaceutical products to improve human health.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of Chinese Academy of Sciences, Wuhan 430074, China. mzzhucn@hotmail.com.
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Zhu
authors.affiliation: Changchun University of Chinese Traditional Medicine, Changchun 130000, China. weiwutian@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Changchun University of Chinese Traditional Medicine, Changchun 130000, China. jiaoaj@hotmail.com.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of Chinese Academy of Sciences, Wuhan 430074, China. yangpf@wbgcas.cn.
authors.forename: Ping-Fang
authors.initials: PF
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of Chinese Academy of Sciences, Wuhan 430074, China. guomq@wbgcas.cn.
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Guo
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology*; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts
keywords: antioxidant activity; lotus leaves; macroporous resin chromatography; mass spectrometry