title: Current Review of Genetically Modified Lactic Acid Bacteria for the Prevention and Treatment of Colitis Using Murine Models.
abstract: Inflammatory Bowel Diseases (IBD) are disorders of the gastrointestinal tract characterized by recurrent inflammation that requires lifelong treatments. Probiotic microorganisms appear as an alternative for these patients; however, probiotic characteristics are strain dependent and each probiotic needs to be tested to understand the underlining mechanisms involved in their beneficial properties. Genetic modification of lactic acid bacteria (LAB) was also described as a tool for new IBD treatments. The first part of this review shows different genetically modified LAB (GM-LAB) described for IBD treatment since 2000. Then, the two principally studied strategies are discussed (i) GM-LAB producing antioxidant enzymes and (ii) GM-LAB producing the anti-inflammatory cytokine IL-10. Different delivery systems, including protein delivery and DNA delivery, will also be discussed. Studies show the efficacy of GM-LAB (using different expression systems) for the prevention and treatment of IBD, highlighting the importance of the bacterial strain selection (with anti-inflammatory innate properties) as a promising alternative. These microorganisms could be used in the near future for the development of therapeutic products with anti-inflammatory properties that can improve the quality of life of IBD patients.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), T4000ILC San Miguel de Tucumán, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: de Moreno de LeBlanc
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), T4000ILC San Miguel de Tucumán, Argentina.
authors.forename: Silvina
authors.identifier: 0000-0002-0285-9586
authors.initials: S
authors.lastname: Del Carmen
authors.affiliation: INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR1319 Micalis, 78350 Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, 78350 Jouy-en-Josas, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chatel
authors.affiliation: Federal University of Minas Gerais (UFMG), 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.affiliation: Federal University of Minas Gerais (UFMG), 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Vasco
authors.identifier: 0000-0002-4775-2280
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR1319 Micalis, 78350 Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, 78350 Jouy-en-Josas, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR1319 Micalis, 78350 Jouy-en-Josas, France ; AgroParisTech, UMR1319 Micalis, 78350 Jouy-en-Josas, France.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), T4000ILC San Miguel de Tucumán, Argentina.
authors.forename: Jean Guy
authors.identifier: 0000-0002-4634-8630
authors.initials: JG
authors.lastname: LeBlanc