title: Inhibition of mouse B16 melanoma by sodium butyrate correlated to tumor associated macrophages differentiation suppression.
abstract: OBJECTIVE
  As one member of the histone deacetylase inhibitor (HDACi) family, Sodium butyrate (NaB) was found out that could be used as a differentiation inducer of much cancer cell. But its effects on tumor microenvironment cells are not well recognized. The goal of this research is to investigate the effect of NaB on B16 melanoma and analysis its relevant mechanism.
  
  
  METHODS
  We observed the effect of sodium butyrate on B16 melanoma in vivo and in vitro. MTT method was performed to detect cell apoptosis rate after treatment. Tumor associated macrophage infiltration condition was detected by flow cytometry. Western-blotting and immunohistochemical method were used to detect the expression of tumor associated macrophage cytokines.
  
  
  RESULTS
  A certain concentration of sodium butyrate could effectively inhibit B16 melanoma growth in vivo and in vitro, and this inhibition effects related to the suppression of tumor associated macrophage differentiation. At the same time we observed the relevant macrophage factors were down-regulated compared to the control.
  
  
  CONCLUSIONS
  Sodium butyrate could effectively inhibit B16 melanoma growth through suppressing tumor associated macrophage proliferation and reduce relevant pro-tumor macrophage factors expression, which may help to promote the clinical study of melanoma epigenetic therapy.
authors.affiliation: Department of Dermatology in Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 611000, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Xiong
authors.affiliation: Department of Dermatology in Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 611000, China.
authors.forename: Yun-Zhu
authors.initials: YZ
authors.lastname: Mou
authors.affiliation: Department of Burn Injury in Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xiang
keywords: B16; Sodium butyrate (NaB); TAMs