title: CpG-ODN Class C Mediated Immunostimulation in Rabbit Model of Trypanosoma evansi Infection.
abstract: CpG oligodeoxynucleotides (CpG-ODN) stimulate immune cells from a wide spectrum of mammalian species. Class C CpG-ODN is relatively stable and has the combined immune effects of both A and B classes of CpG-ODN. Trypanosoma evansi produces the state of immuno-suppression in the infected hosts. The current chemotherapeutic agents against this parasite are limited in number and usually associated with severe side effects. The present work aimed to determine the immunostimulatory effects of CpG-ODN class C in T. evansi infected rabbits. Rabbits inoculated with CpG C and challenged with T. evansi resulted in delayed onset of clinical signs with reduced severity in comparison to that of T. evansi infected rabbits. The treatment also enhanced humoral immune responses. Histopathological findings in liver and spleen revealed enhancement of mononuclear cell infiltration and secondary B cell follicles. These results demonstrate that CpG-ODN class C, has immunostimulatory properties in rabbit model of trypanosomosis. The use of booster doses or sustained delivery of CpG-ODN will further elucidate the prolonged CpG-ODN generated immune responses.
authors.affiliation: National Research Centre on Equines, Sirsa road, Hisar-125001, Haryana, India; Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125001, Haryana, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125001, Haryana, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: National Research Centre on Equines, Sirsa road, Hisar-125001, Haryana, India.
authors.forename: Balvinder Kumar
authors.initials: BK
authors.lastname: Manuja
authors.affiliation: National Research Centre on Equines, Sirsa road, Hisar-125001, Haryana, India.
authors.forename: Harisankar
authors.initials: H
authors.lastname: Singha
authors.affiliation: Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125001, Haryana, India.
authors.forename: Anshu
authors.initials: A
authors.lastname: Sharma
authors.affiliation: National Research Centre on Equines, Sirsa road, Hisar-125001, Haryana, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Virmani
authors.affiliation: National Research Centre on Equines, Sirsa road, Hisar-125001, Haryana, India.
authors.forename: Suresh Chandra
authors.initials: SC
authors.lastname: Yadav
authors.affiliation: National Research Centre on Equines, Sirsa road, Hisar-125001, Haryana, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Manuja
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011817:Rabbits; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides