title: Effect of tannic and gallic acids alone or in combination with carbenicillin or tetracycline on Chromobacterium violaceum CV026 growth, motility, and biofilm formation.
abstract: Chromobacterium violaceum is an opportunistic pathogen that causes infections that are difficult to treat. The goal of this research was to evaluate the effect of selected tannins (tannic acid (TA) and gallic acid (GA)) on bacterial growth, motility, antibiotic (carbenicillin, tetracycline) susceptibility, and biofilm formation. Both tannins, particularly TA, impaired bacterial growth levels and swimming motilities at sub-minimum inhibitory concentrations (sub-MICs). In combination with tannins, antibiotics showed increased MICs, suggesting that tannins interfered with antibacterial activity. Sub-MICs of tetracycline or TA alone enhanced biofilm formation of C. violaceum; however, in combination, these compounds inhibited biofilm formation. In contrast, carbenicillin at sub-MICs was effective in inhibiting C. violaceum biofilm formation; however, in combination with lower concentrations of TA or GA, biofilms were enhanced. These results provide insights into the effects of tannins on C. violaceum growth and their varying interaction with antibiotics used to target C. violaceum infections.
authors.affiliation: Department of Chemical Engineering, McGill University, Montréal, QC H3A 0C5, Canada.|Department of Chemical Engineering, McGill University, Montréal, QC H3A 0C5, Canada.
authors.forename: Devendra H
authors.initials: DH
authors.lastname: Dusane
authors.affiliation: Department of Chemical Engineering, McGill University, Montréal, QC H3A 0C5, Canada.|Department of Chemical Engineering, McGill University, Montréal, QC H3A 0C5, Canada.
authors.forename: Che
authors.initials: C
authors.lastname: O'May
authors.affiliation: Department of Chemical Engineering, McGill University, Montréal, QC H3A 0C5, Canada.|Department of Chemical Engineering, McGill University, Montréal, QC H3A 0C5, Canada.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Tufenkji
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002228:Carbenicillin / Q000494:pharmacology*; D002861:Chromobacterium / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D004357:Drug Synergism; D005707:Gallic Acid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013634:Tannins / Q000494:pharmacology*; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013634:Tannins; D005707:Gallic Acid; D013752:Tetracycline; D002228:Carbenicillin
keywords: Chromobacterium violaceum; antibiotic; antibiotique; biofilm; motility; motilité; tannins