title: Comparative study of dermal components and plasma TGF-β1 levels in Slc39a13/Zip13-KO mice.
abstract: Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a crucial role in formation of the extracellular matrix. It has been reported that the loss of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1 signaling pathway is observed, although the relationship between the dermis abnormalities and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS. Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the amount of collagen were decreased, and there were very few collagen fibrils with diameters of more than 150 nm in Zip13-KO mice dermis. We also found that the TGF-β1 level was significantly higher in Zip13-KO mice serum. These results suggest that collagen synthesis and collagen fibril fusion might be impaired in Zip13-KO mice and that the possible decrease of decorin level by reduction of the DS ratio probably caused an increase of free TGF-β1 in Zip13-KO mice. In conclusion, skin fragility due to defective ZIP13 protein may be attributable to impaired extracellular matrix synthesis accompanied by abnormal peripheral TGF-β homeostasis.
authors.affiliation: Department of Veterinary Microanatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Hirose
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ogura
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tanaka
authors.forename: Jun
authors.initials: J
authors.lastname: Minaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Fukada
authors.forename: Yoh-ichi
authors.initials: Y
authors.lastname: Koyama
authors.forename: Kazushige
authors.initials: K
authors.lastname: Takehana
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D003094:Collagen / Q000648:ultrastructure; D004195:Disease Models, Animal; D004535:Ehlers-Danlos Syndrome / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005838:Genotype; D006025:Glycosaminoglycans / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010009:Osteochondrodysplasias / Q000097:blood / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure; D053773:Transforming Growth Factor beta1 / Q000097:blood* / Q000235:genetics / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D006025:Glycosaminoglycans; C000611124:Slc39a13 protein, mouse; D053773:Transforming Growth Factor beta1; D003094:Collagen