title: Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari.
abstract: Bacteriocin producing strain BDU3 was isolated from a traditional fermented fish of Manipur Ngari. The strain BDU3 was identified as Bacillus coagulans by phenotypic and genotypic characterization. The BDU3 produced novel bacteriocin, which showed an antimicrobial spectrum toward a wide spectrum of food borne, and closely related pathogens with a MIC that ranged between 0.5 and 2.5 μg/mL. The isolate was able to tolerate pH as low as 2.0 and up to 0.2% bile salt concentration. Three step purification was employed to increase the specific activity of the antimicrobial compound. The fractions were further chromatographed by Rp-HPLC C-18 column and the purified bacteriocin had a specific activity of ∼8500 AU/mg. However, the potency of bacteriocin was susceptible to digestion with Proteinase K, Pepsin, SDS, EDTA and Urea. Molecular mass of purified bacteriocin was found to be 1.4 kDa using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). The functional group was revealed by FTIR analysis. The cytotoxicity assay (MTT) using purified bacteriocin showed 2 times lower EC50 values compared to SDS. This is the smaller bacteriocin ever reported before from B. coagulans with greater antimicrobial potency with lower cytotoxicity. This bacteriocin raises the possibilities to be used as a biopreservative in food industries.
authors.affiliation: Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India; Department of Biotechnology, Nandha Arts and Science College, Bharathiar University, Erode 638009, India.
authors.forename: Kaja
authors.initials: K
authors.lastname: Abdhul
authors.affiliation: Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Ganesh
authors.affiliation: Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
authors.forename: Santhanam
authors.initials: S
authors.lastname: Shanmughapriya
authors.affiliation: Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Vanithamani
authors.affiliation: Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
authors.forename: Murugesan
authors.initials: M
authors.lastname: Kanagavel
authors.affiliation: Microbial Technology Laboratory, Department of Marine Biotechnology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India.
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Anbarasu
authors.affiliation: Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India. Electronic address: natarajaseenivasan@rediffmail.com.
authors.forename: Kalimuthusamy
authors.initials: K
authors.lastname: Natarajaseenivasan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004492:Edetic Acid / Q000737:chemistry; D019286:Endopeptidase K / Q000737:chemistry; D005396:Fish Products / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010434:Pepsin A / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012967:Sodium Dodecyl Sulfate; D014508:Urea / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D012336:RNA, Ribosomal, 16S; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D004492:Edetic Acid; D019286:Endopeptidase K; D010434:Pepsin A
keywords: Bacillus coagulans; Bacteriocin; FTIR; MALDI-TOF; Ngari; Probiotics