title: Bax and Bif-1 proteins interact on Bilayer Lipid Membrane and form pore.
abstract: Bax and Bax interacting factor-1(Bif-1) are cytosolic proteins, which translocate towards mitochondria during mitochondria-mediated apoptosis. Bif-1 has been identified to co-immunoprecipitate with Bax in apoptotic cells. We have studied the interaction of Bax and Bif-1 on Bilayer Lipid Membrane (BLM) through electrophysiological experiments. It has been observed that Bax-Bif-1 equimolar mixture can form a pore. The pore conductance is in the range of 4.96-5.41 nS. It also displays a sub-state with a conductance of 2.6 nS. No pore activity is observed on BLM when monomeric Bax and Bif-1 proteins are tested independently. The above-mentioned pore forming activity could be relevant in mitochondria-mediated apoptosis.
authors.affiliation: Department of Biophysics, University of Delhi South Campus, New Delhi, India.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Biophysics, University of Delhi South Campus, New Delhi, India. Electronic address: profsubhendu@gmail.com.
authors.forename: Subhendu
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D008051:Lipid Bilayers; D018408:Patch-Clamp Techniques; D011485:Protein Binding; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008051:Lipid Bilayers; C429293:SH3GLB1 protein, human; D051028:bcl-2-Associated X Protein
keywords: Bax; Bif-1; Bilayer electrophysiology; Mitochondria-mediated apoptosis; Pore