title: The structure of haemoglobin bound to the haemoglobin receptor IsdH from Staphylococcus aureus shows disruption of the native α-globin haem pocket.
abstract: Staphylococcus aureus is a common and serious cause of infection in humans. The bacterium expresses a cell-surface receptor that binds to, and strips haem from, human haemoglobin (Hb). The binding interface has previously been identified; however, the structural changes that promote haem release from haemoglobin were unknown. Here, the structure of the receptor-Hb complex is reported at 2.6 Å resolution, which reveals a conformational change in the α-globin F helix that disrupts the haem-pocket structure and alters the Hb quaternary interactions. These features suggest potential mechanisms by which the S. aureus Hb receptor induces haem release from Hb.
authors.affiliation: University of Tasmania, Hobart, TAS 7000, Australia.
authors.forename: Claire F
authors.initials: CF
authors.lastname: Dickson
authors.affiliation: University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Jacques
authors.affiliation: UCLA, Los Angeles, CA 90095, USA.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Clubb
authors.affiliation: University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: J Mitchell
authors.initials: JM
authors.lastname: Guss
authors.affiliation: University of Tasmania, Hobart, TAS 7000, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Gell
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000737:chemistry*; D055542:alpha-Globins / Q000737:chemistry*
chemical_list: D000942:Antigens, Bacterial; D006454:Hemoglobins; C512792:IsdH protein, Staphylococcus aureus; D011956:Receptors, Cell Surface; D055542:alpha-Globins
keywords: NEAT domain; Staphylococcus aureus; haemoglobin; iron-regulated surface determinant