title: MicroRNAs Signature in IL-2-Induced CD4+ T Cells and Their Potential Targets.
abstract: MicroRNAs (miRNAs) are a class of small non-coding RNAs regulated gene expression at the post-transcriptional level. Many studies have investigated role of miRNAs in the biological processes such as proliferation, apoptosis, differentiation, and development. To evaluate role of miRNAs in proliferation and death of T cell, we performed miRNA profiling in activated CD4+ T cells after IL-2 induction and depletion. Proliferation rate of IL-2-induced cells was measured by MTT assay. Then quantitative RT-PCR arrays on 739 miRNAs revealed up- and down-regulation of 170 miRNAs in IL-2-induced CD4+ T cells relative to IL-2-depleted ones. In addition, in silico analysis predicted miRNA's potential targets in pathways such as JAK/STAT and PI3K pathways. JAK1 expression, a potential target of modulated miRNAs, was decreased in IL-2-depleted cells. This study suggests that clonal expansion is regulated by miRNAs in the absence or presence of IL-2 by targeting genes implicated in JAK/STAT and PI3K pathways.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran, n_ranji@iaurasht.ac.ir.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Ranji
authors.forename: Majid
authors.initials: M
authors.lastname: Sadeghizadeh
authors.forename: Morteza
authors.initials: M
authors.lastname: Karimipoor
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Roohollah
authors.initials: R
authors.lastname: Nakhaei Sistani
authors.forename: Seyed Hassan
authors.initials: SH
authors.lastname: Paylakhi
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D019295:Computational Biology; D020869:Gene Expression Profiling; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D053613:Janus Kinase 1 / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D058990:Molecular Targeted Therapy; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D007376:Interleukin-2; D035683:MicroRNAs; D012333:RNA, Messenger; D050791:STAT Transcription Factors; D019869:Phosphatidylinositol 3-Kinases; D053613:Janus Kinase 1