title: Mitogen activated protein kinase pathway-dependent effects of platelet-derived growth factor on migration of trophectoderm cells.
abstract: Successful development of the conceptus and implantation requires an intimate trophic connection between maternal uterus and conceptus mediated by local regulators including growth factors. Platelet-derived growth factor (PDGF) acts as a chemotactic factor for a variety of cell types. Current studies have determined that PDGF participates in rapid growth and development of cleavage stage embryos, but PDGF-induced effects on the growth and development of peri-implantation conceptus remains unknown. In the present study, PDGF induced phosphorylation of ERK1/2, AKT and RPS6 proteins in porcine trophectoderm (pTr) cells in a dose- and time-dependent manner. Addition of U0126 (an inhibitor of ERK1/2) or LY294002 (a PI3K inhibitor) blocked PDGF-induced effects on phosphorylation of signaling proteins. Combinations of PDGF and U0126 decreased PDGF-induced p-ERK1/2 and p-AKT1, but combinations of PDGF and LY294002 blocked only PDGF-induced AKT phosphorylation. Furthermore, PDGF significantly induced pTr cell migration and these stimulatory effects were blocked by U0126 and LY294002. Immunoreactive p-ERK1/2 and p-RPS6 proteins were abundant in pTr cells treated with PDGF, but U0126 reduced PDGF-induced p-ERK1/2 and p-RPS6 levels to basal amounts. Present study suggests that PDGF secreted into the maternal-conceptus microenvironment stimulates pTr cell migration through signal transduction cascades mediated by the ERK1/2 MAPK and AKT1 pathways.
authors.affiliation: Department of Animal Resources Science, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Wooyoung
authors.initials: W
authors.lastname: Jeong
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Gwonhwa
authors.initials: G
authors.lastname: Song
authors.affiliation: Department of Animal Resources Science, Dankook University, Cheonan 330-714, Republic of Korea. Electronic address: jinyoungkim@dankook.ac.kr.
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D004475:Ectoderm / Q000166:cytology*; D020935:MAP Kinase Signaling System; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D013552:Swine; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D010982:Platelet-Derived Growth Factor
keywords: Migration; PDGF; Peri-implantation; Trophectoderm