title: Evaluation of the role of autogenous bone-marrow-derived mesenchymal stem cell transplantation for the repair of mandibular bone defects in rabbits.
abstract: The repair of craniofacial bony defects by traditional grafting techniques requires substantial time and effort, with associated morbidity. Tissue engineering has therefore become a novel approach targeting application for bone regeneration. This study used the rabbit model for radiographic and histological evaluation of bone bioengineering for mandibular defects reconstruction using only β-tricalcium phosphate (β-TCP) and, when loaded with autogenous; bone marrow-derived undifferentiated mesenchymal stem cells (BM-MSCs). Critical-sized defects (10 × 15 mm) were created unilaterally in the mandibular body region of each rabbit (n = 16), to be filled with the BM-MSCs/β-TCP constructs for the study group (group I) (n1 = 8) and with scaffold devoid of cells for the control group (group II) (n2 = 8). Two rabbits from each group were sacrificed after healing periods of 2, 4, 12, and 24 weeks. The results revealed that the BM-MSCs endowed β-TCP scaffold with a better and more rapid bone regenerating potential: since the first evaluation period of 2 weeks, the regenerated bone tissue in group I was more mature, denser and homogeneously distributed. From these findings we could infer that the bone regeneration process was jump-started within the study group cases, which led to better quality of regenerated bone.
authors.affiliation: Tanta University, Egypt.
authors.forename: Khaled Abd-Elhamid
authors.initials: KA
authors.lastname: Saad
authors.affiliation: Tanta University, Egypt. Electronic address: infrol@yahoo.com.
authors.forename: Ahmed Gamal Taha
authors.initials: AG
authors.lastname: Abu-Shahba
authors.affiliation: Tanta University, Egypt.
authors.forename: Ezzat Abd-Elaziz
authors.initials: EA
authors.lastname: El-Drieny
authors.affiliation: Tanta University, Egypt.
authors.forename: Mohamed Saad
authors.initials: MS
authors.lastname: Khedr
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration; D008297:Male; D008334:Mandible / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
keywords: Bone marrow; Mandibular bone defects; Mesenchymal stem cells; New Zealand White rabbits; Repair; β-TCP scaffold