title: Interdisciplinary approach to cell-biomaterial interactions: biocompatibility and cell friendly characteristics of RKKP glass-ceramic coatings on titanium.
abstract: In this work, titanium (Ti) supports have been coated with glass-ceramic films for possible applications as biomedical implant materials in regenerative medicine. For the film preparation, a pulsed laser deposition (PLD) technique has been applied. The RKKP glass-ceramic material, used for coating deposition, was a sol-gel derived target of the following composition: Ca-19.4, P-4.6, Si-17.2, O-43.5, Na-1.7, Mg-1.3, F-7.2, K-0.2, La-0.8, Ta-4.1 (all in wt%). The prepared coatings were compact and uniform, characterised by a nanometric average surface roughness. The biocompatibility and cell-friendly properties of the RKKP glass-ceramic material have been tested. Cell metabolic activity and proliferation of human colon carcinoma CaCo-2 cells seeded on RKKP films showed the same exponential trend found in the control plastic substrates. By the phalloidin fluorescence analysis, no significant modifications in the actin distribution were revealed in cells grown on RKKP films. Moreover, in these cells a high mRNA expression of markers involved in protein synthesis, proliferation and differentiation, such as villin (VIL1), alkaline phosphatase (ALP1), β-actin (β-ACT), Ki67 and RPL34, was recorded. In conclusion, the findings, for the first time, demonstrated that the RKKP glass-ceramic material allows the adhesion, growth and differentiation of the CaCo-2 cell line.
authors.affiliation: Institute of Translational Pharmacology (IFT-CNR), Via del Fosso del Cavaliere, 100, 00133 Rome, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Ledda
authors.forename: Angela
authors.initials: A
authors.lastname: De Bonis
authors.forename: Francesca Romana
authors.initials: FR
authors.lastname: Bertani
authors.forename: Ilaria
authors.initials: I
authors.lastname: Cacciotti
authors.forename: Roberto
authors.initials: R
authors.lastname: Teghil
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Lolli
authors.forename: Antonio
authors.initials: A
authors.lastname: Ravaglioli
authors.forename: Antonella
authors.initials: A
authors.lastname: Lisi
authors.forename: Julietta V
authors.initials: JV
authors.lastname: Rau
mesh_terms: D000199:Actins / Q000378:metabolism; D018938:Caco-2 Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D008422:Materials Testing; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044968:Regenerative Medicine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D000199:Actins; D020099:Coated Materials, Biocompatible; D012333:RNA, Messenger; C031323:bio-vitroceramic AP40; C023506:Glass ceramics; D014025:Titanium