title: Characterization of Adelphocoris suturalis (Hemiptera: Miridae) Transcriptome from Different Developmental Stages.
abstract: Adelphocoris suturalis is one of the most serious pest insects of Bt cotton in China, however its molecular genetics, biochemistry and physiology are poorly understood. We used high throughput sequencing platform to perform de novo transcriptome assembly and gene expression analyses across different developmental stages (eggs, 2(nd) and 5(th) instar nymphs, female and male adults). We obtained 20 GB of clean data and revealed 88,614 unigenes, including 23,830 clusters and 64,784 singletons. These unigene sequences were annotated and classified by Gene Ontology, Clusters of Orthologous Groups, and Kyoto Encyclopedia of Genes and Genomes databases. A large number of differentially expressed genes were discovered through pairwise comparisons between these developmental stages. Gene expression profiles were dramatically different between life stage transitions, with some of these most differentially expressed genes being associated with sex difference, metabolism and development. Quantitative real-time PCR results confirm deep-sequencing findings based on relative expression levels of nine randomly selected genes. Furthermore, over 791,390 single nucleotide polymorphisms and 2,682 potential simple sequence repeats were identified. Our study provided comprehensive transcriptional gene expression information for A. suturalis that will form the basis to better understanding of development pathways, hormone biosynthesis, sex differences and wing formation in mirid bugs.
authors.affiliation: Henan Key Laboratory of Crop Pest Control, MOA Key Regional Crop Integrated Pest Management (IPM) Laboratory in Southern Part of Northern China, International Joint Research Laboratory for Crop Protection of Henan Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Tian
authors.affiliation: CSIRO, Clunies Ross Street, ACT 2601, Australia.
authors.forename: Wee
authors.initials: W
authors.lastname: Tek Tay
authors.affiliation: Henan Key Laboratory of Crop Pest Control, MOA Key Regional Crop Integrated Pest Management (IPM) Laboratory in Southern Part of Northern China, International Joint Research Laboratory for Crop Protection of Henan Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Feng
authors.affiliation: Henan Key Laboratory of Crop Pest Control, MOA Key Regional Crop Integrated Pest Management (IPM) Laboratory in Southern Part of Northern China, International Joint Research Laboratory for Crop Protection of Henan Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Henan Key Laboratory of Crop Pest Control, MOA Key Regional Crop Integrated Pest Management (IPM) Laboratory in Southern Part of Northern China, International Joint Research Laboratory for Crop Protection of Henan Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Henan Key Laboratory of Crop Pest Control, MOA Key Regional Crop Integrated Pest Management (IPM) Laboratory in Southern Part of Northern China, International Joint Research Laboratory for Crop Protection of Henan Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D005260:Female; D006430:Hemiptera / Q000235:genetics / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008018:Life Cycle Stages / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D019476:Insect Proteins