title: Understanding liver immunology using intravital microscopy.
abstract: The liver has come a long way since it was considered only a metabolic organ attached to the gastrointestinal tract. The simultaneous ascension of immunology and intravital microscopy evidenced the liver as a central axis in the immune system, controlling immune responses to local and systemic agents as well as disease tolerance. The multiple hepatic cell populations are organized in a vascular environment that promotes intimate cellular interactions, including initiation of innate and adaptive immune responses, rapid leukocyte recruitment, pathogen clearance and production of a variety of immune mediators. In this review, we focus on the advances in liver immunology supported by intravital microscopy in diseases such as isquemia/reperfusion, acute liver injury and infections.
authors.affiliation: Laboratório de Imunobiofotônica, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Pedro Elias
authors.initials: PE
authors.lastname: Marques
authors.affiliation: Laboratório de Imunobiofotônica, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
authors.forename: André Gustavo
authors.initials: AG
authors.lastname: Oliveira
authors.affiliation: Nikon Instruments, Inc., Melville, NY, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Chang
authors.affiliation: Departamento de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Heitor Affonso
authors.initials: HA
authors.lastname: Paula-Neto
authors.affiliation: Laboratório de Imunobiofotônica, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil. Electronic address: menezesgb@ufmg.br.
authors.forename: Gustavo Batista
authors.initials: GB
authors.lastname: Menezes
mesh_terms: D055371:Acute Lung Injury / Q000473:pathology; D000818:Animals; D019446:Endotoxemia / Q000473:pathology; D006801:Humans; D000069416:Intravital Microscopy / Q000379:methods*; D008099:Liver / Q000098:blood supply / Q000276:immunology* / Q000469:parasitology / Q000601:surgery; D015427:Reperfusion Injury / Q000473:pathology
keywords: In vivo imaging; Liver immunology; Liver infection; Liver intravital microscopy; Sterile injury