title: Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles.
abstract: In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between the catalytic activity, measured by pH-stat titration, and the change in morphology and nanostructure determined by cryogenic transmission electron microscopy and synchrotron small angle X-ray diffraction. The initial lipase catalyzed LCNP hydrolysis rate is approximately 25% higher for large 350nm nanoparticles compared to the small 190nm particles, which is attributed to the increased number of structural defects on the particle surface. At pH 8.0 and 8.4 bicontinuous Im3m cubic LCNPs transform into "sponge"-like assemblies and disordered multilamellar onion-like structures upon exposure to lipase. At pH 6.5 and 7.5 lipolysis induced phase transitions of the inner core of the particles, following the sequence Im3m cubic → reversed hexagonal → reversed micellar Fd3m cubic → reversed micelles. These transitions to the liquid crystalline phases with higher negative curvature of the lipid/water interface were found to trigger protonation of the oleic acid produced during lipase catalyzed reaction. The increase curvature of the reversed discrete micellar cubic phase was suggested to cause an increase in the oleic acid pKa to a larger value observed by pH-stat titration.
authors.affiliation: Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-20506 Malmö, Sweden; Camurus AB, Ideon Science Park, Gamma Building, Sölvegatan 41, SE-22370 Lund, Sweden.
authors.forename: Justas
authors.initials: J
authors.lastname: Barauskas
authors.affiliation: Physical Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden; Department of Biochemistry and Structural Biology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Anderberg
authors.affiliation: Novozymes A/S, Smørmosevej 25, DK-2880 Bagsvaerd, Denmark.
authors.forename: Allan
authors.initials: A
authors.lastname: Svendsen
authors.affiliation: Physical Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden. Electronic address: Tommy.Nylander@fkem1.lu.se.
authors.forename: Tommy
authors.initials: T
authors.lastname: Nylander
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002384:Catalysis; D003460:Crystallization; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D053758:Nanoparticles; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D008049:Lipase
keywords: Cubic phase; Hydrolysis; Lipase; Lipid liquid crystalline nanoparticles; X-ray diffraction; pH-stat titration