title: Dye-Free Porcine Model of Experimental Branch Retinal Vein Occlusion: A Suitable Approach for Retinal Proteomics.
abstract: Branch retinal vein occlusion induces complex biological processes in the retina that are generated by a multitude of interacting proteins. These proteins and their posttranslational modifications can effectively be studied using modern proteomic techniques. However, no method for studying large-scale protein changes following branch retinal vein occlusion has been available until now. Obtainment of retinal tissue exposed to branch retinal vein occlusion is only available through experimental animal models. Traditional models of experimental branch retinal vein occlusion require the use of Rose Bengal dye combined with argon laser photocoagulation. The use of Rose Bengal dye is problematic in proteomic studies as the dye can induce multiple protein modifications when irradiated. This paper presents a novel technique for proteomic analysis of porcine retinal tissue with branch retinal vein occlusion combining a dye-free experimental model with label-free liquid chromatography mass spectrometry based proteomics.
authors.affiliation: Department of Ophthalmology, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark ; Biomedical Research Laboratory, Aalborg University Hospital, Ladegårdsgade 3, 9000 Aalborg, Denmark.
authors.forename: Lasse Jørgensen
authors.initials: LJ
authors.lastname: Cehofski
authors.affiliation: Department of Ophthalmology, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark.
authors.forename: Anders
authors.initials: A
authors.lastname: Kruse
authors.affiliation: Biomedical Research Laboratory, Aalborg University Hospital, Ladegårdsgade 3, 9000 Aalborg, Denmark ; Department of Heart and Lung Surgery, Center for Cardiovascular Research, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark.
authors.forename: Benedict
authors.initials: B
authors.lastname: Kjærgaard
authors.affiliation: Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3, Building B, 9220 Aalborg, Denmark.
authors.forename: Allan
authors.initials: A
authors.lastname: Stensballe
authors.affiliation: Department of Biomedicine, Aarhus University, Ole Worms Allé 3, Building 1182, Room 024, 8000 Aarhus C, Denmark.
authors.forename: Bent
authors.initials: B
authors.lastname: Honoré
authors.affiliation: Department of Ophthalmology, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Vorum