title: Regulation analysis of AcMNPV-mediated expression of a Chinese scorpion neurotoxin under the IE1, P10 and PH promoter in vivo and its use as a potential bio-insecticide.
abstract: OBJECTIVE
  To analyze the regulation mechanism of AcMNPV (Autographa californica multicapsid nucleopolyhedrovirus)-mediated expression of BmK IT under IE1, P10 and PH promoters in the larva of Heliothis armigera..
  
  
  RESULTS
  The transcription level of BmK IT gene in midgut and epidermal tissue was analyzed by quantitative PCR. The start time of transcription of recombinant BmK IT gene was early under the regulation of IE promoter, whereas transcription of BmK IT was high under the regulation of P10 promoter in the midgut tissue of infected larvae. TdT-UTP nick-end labeling (TUNEL) assay showed the degree of apoptotic cell death in the midgut tissue of AcMNPV-BmK IT-transfected insect larvae was higher than that in the AcMNPV treatment group at 8 h post-infection. The time-effect relationship between the insect's humoral immunity and regulation of promoters was confirmed in the phenoloxidase activity assay.
  
  
  CONCLUSIONS
  The anti-insect mechanism and regulation of different promoters in AcMNPV-BmK IT at molecular and cellular levels provide an experimental basis for the development of recombinant baculovirus biopesticides.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China. yjfu@sxu.edu.cn.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.affiliation: University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000502:physiology / Q000821:virology; D007915:Lepidoptera / Q000502:physiology / Q000821:virology; D009498:Neurotoxins / Q000235:genetics / Q000378:metabolism*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D007306:Insecticides; D009498:Neurotoxins; D011994:Recombinant Proteins
keywords: Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV); Baculovirus; Bio-insecticide; Buthus martensii Karsch insect toxin (BmK IT); Heliothis armigera; Scorpion neurotoxin