title: Isolation of phenazine 1,6-di-carboxylic acid from Pseudomonas aeruginosa strain HRW.1-S3 and its role in biofilm-mediated crude oil degradation and cytotoxicity against bacterial and cancer cells.
abstract: Pseudomonas sp. has long been known for production of a wide range of secondary metabolites during late exponential and stationary phases of growth. Phenazine derivatives constitute a large group of secondary metabolites produced by microorganisms including Pseudomonas sp. Phenazine 1,6-di-carboxylic acid (PDC) is one of such metabolites and has been debated for its origin from Pseudomonas sp. The present study describes purification and characterization of PDC isolated from culture of a natural isolate of Pseudomonas sp. HRW.1-S3 while grown in presence of crude oil as sole carbon source. The isolated PDC was tested for its effect on biofilm formation by another environmental isolate of Pseudomonas sp. DSW.1-S4 which lacks the ability to produce any phenazine compound. PDC showed profound effect on both planktonic as well as biofilm mode of growth of DSW.1-S4 at concentrations between 5 and 20 μM. Interestingly, PDC showed substantial cytotoxicity against three cancer cell lines and against both Gram-positive and Gram-negative bacteria. Thus, the present study not only opens an avenue to understand interspecific cooperation between Pseudomonas species which may lead its applicability in bioremediation, but also it signifies the scope of future investigation on PDC for its therapeutic applications.
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, 741246, India.|Centre for Environmental Science and Engineering (CESE), Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Debdeep
authors.initials: D
authors.lastname: Dasgupta
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, 741246, India.
authors.forename: Abhinash
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, 741246, India.
authors.forename: Balaram
authors.initials: B
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, 741246, India. senguptk@iiserkol.ac.in.
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Sengupta
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D001711:Biotransformation; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D010578:Petroleum / Q000378:metabolism*; D010619:Phenazines / Q000302:isolation & purification* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010578:Petroleum; D010619:Phenazines; C019040:phenazine-1,6-dicarboxylate
keywords: Biofilm; Cytotoxicity; Oil degradation; Phenazine 1,6-di-carboxylic acid; Pseudomonas; Skewness