title: A sensitive electrochemiluminescence cytosensor for quantitative evaluation of epidermal growth factor receptor expressed on cell surfaces.
abstract: A sensitive electrochemiluminescence (ECL) strategy for evaluating the epidermal growth factor receptor (EGFR) expression level on cell surfaces was designed by integrating the specific recognition of EGFR expressed on MCF-7 cell surfaces with an epidermal growth factor (EGF)-funtionalized CdS quantum dots (CdSQDs)-capped magnetic bead (MB) probe. The high sensitivity of ECL probe of EGF-funtionalized CdSQD-capped-MB was used for competitive recognition with EGFR expressed on cell surfaces with recombinant EGFR protein. The changes of ECL intensity depended on both the cell number and the expression level of EGFR receptor on cell surfaces. A wide linear response to cells ranging from 80 to 4×10(6)cellsmL(-1) with a detection limit of 40cellsmL(-1) was obtained. The EGF-cytosensor was used to evaluate EGFR expression levels on MCF-7 cells, and the average number of EGFR receptor on single MCF-7 cells was 1.35×10(5) with the relative standard deviation of 4.3%. This strategy was further used for in-situ and real-time evaluating EGFR receptor expressed on cell surfaces in response to drugs stimulation at different concentration and incubation time. The proposed method provided potential applications in the detection of receptors on cancer cells and anticancer drugs screening.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Shaolian
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address: typh@jnu.edu.cn.
authors.forename: Peihui
authors.initials: P
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004815:Epidermal Growth Factor / Q000737:chemistry; D066246:ErbB Receptors / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D058185:Magnetite Nanoparticles; D013440:Sulfides; C034939:cadmium sulfide; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
keywords: EGF-cytosensor; Electrochemiluminescence; Epidermal growth factor receptor