title: Characterization of acidic polysaccharides from the mollusks through acid hydrolysis.
abstract: Uronic acid-containing polysaccharides (UACPs) including glycosaminoglycans (GAGs) exist widely in nature. Herein we propose an elegant methodology to identify UACPs by analyzing their disaccharides produced from the acid hydrolysis using HPLC-MS(n) upon 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. Based on the optimization of experimental conditions by the single factor experiment and orthogonal test design, the combination of 1.3M TFA at 105°C for 3h is found to be the optimum. Subsequently, these conditions were applied to investigate the distribution of UACPs in 20 selected species of edible Bivalvia and Gastropoda. PMP-disaccharides derived from UACPs in mollusks were identified by comparing the retention time and mass spectra with those of the reference PMP-disaccharides from hyaluronic acid (HA), chondroitin sulfate (CS), heparin (HP), and AGSP with →4)-GlcA(1→2)-Man(1→ repeating units. The analysis reveals the prevalence of CS in the shellfishes as well as the HP, and existence of three non-GAG UACPs in 7 mollusks.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Jiuling
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Chengrong
authors.initials: C
authors.lastname: Wen
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Teng
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address: songs1008@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address: zhubeiwei@163.com.
authors.forename: Beiwei
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002809:Chondroitin Sulfates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis; D006025:Glycosaminoglycans / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006868:Hydrolysis; D013058:Mass Spectrometry; D008974:Mollusca; D011134:Polysaccharides / Q000737:chemistry*; D014574:Uronic Acids / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D006025:Glycosaminoglycans; D011134:Polysaccharides; D014574:Uronic Acids; D006493:Heparin; D002809:Chondroitin Sulfates
keywords: Disaccharide; Glycosaminoglycan; HPLC–MS; Shellfish; UACPs