title: VEGF gene transfected umbilical cord mesenchymal stem cells transplantation improve the lower limb vascular lesions of diabetic rats.
abstract: BACKGROUND
  Previous studies had explored the therapeutic effect of hUC-MSCs transplantation on ischemia; in this study, we further assessed the effectiveness of VEGF over-expressed hUC-MSCs cells transplantation on vascular proliferation in lower limb ischemia model in type 2 diabetic rats.
  
  
  METHODS
  hUC-MSCs cells were over-expressed with VEGF, and transplanted to lower limb ischemia rats model of type 2 diabetes.
  
  
  RESULTS
  VEGF over-expression increased the hUC-MSCs cells proliferation activity and VEGF secretion. VEGF gene transfected hUC-MSCs transplantation increased VEGF expression at a high level throughout 4weeks in skeletal muscle tissues of rats. Importantly, the vascular proliferation and blood perfusion of VEGF over-expressed hUC-MSCs transplanted limb were significantly improved compared with those of control group. The expression levels of ERK, AKT, MMP2 and MMP9 in VEGF over-expressed hUC-MSCs transplantation group increased dramatically compared with control group, while TIMP1 and TIMP2 expression had no significant change.
  
  
  CONCLUSIONS
  VEGF over-expressed hUC-MSCs transplantation was more effective to stimulate angiogenesis and increase blood perfusion than the simply hUC-MSCs transplantation, as maybe a new choice to improve the lower limb vascular lesions of diabetics.
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Gan
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, China. Electronic address: sguangyao2@163.com.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Song
authors.affiliation: Department of endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000601:surgery*; D003925:Diabetic Angiopathies / Q000517:prevention & control / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012039:Regional Blood Flow / Q000502:physiology; D012680:Sensitivity and Specificity; D014162:Transfection; D014470:Umbilical Cord; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Diabetes; Lower limb vascular lesion; Stem cells; Transplantation; VEGF