title: Expression and partial characterisation of rabbit haemorrhagic disease virus non-structural proteins.
abstract: The intracellular replication and molecular virulence mechanisms of Rabbit haemorrhagic disease virus (RHDV) are poorly understood, mainly due to the lack of an effective cell culture system for this virus. To increase our understanding of RHDV molecular biology, the subcellular localisation of recombinant non-structural RHDV proteins was investigated in transiently transfected rabbit kidney (RK-13) cells. We provide evidence for oligomerisation of p23, and an ability of the viral protease to cleave the p16:p23 junction in trans, outside the context of the nascent polyprotein chain. Notably, expression of the viral polymerase alone and in the context of the entire RHDV polyprotein resulted in a redistribution of the Golgi network. This suggests that, similar to other positive-strand RNA viruses, RHDV may recruit membranes of the secretory pathway during replication, and that the viral polymerase may play a critical role during this process.
authors.affiliation: CSIRO Biosecurity Flagship, ACT, Australia; Invasive Animals Cooperative Research Centre, University of Canberra, ACT, Australia; Health Research Institute, University of Canberra, ACT, Australia.
authors.forename: Nadya
authors.initials: N
authors.lastname: Urakova
authors.affiliation: CSIRO Biosecurity Flagship, ACT, Australia; Invasive Animals Cooperative Research Centre, University of Canberra, ACT, Australia; Health Research Institute, University of Canberra, ACT, Australia; Institute for Applied Ecology, University of Canberra, ACT, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Frese
authors.affiliation: CSIRO Biosecurity Flagship, ACT, Australia.
authors.forename: Robyn N
authors.initials: RN
authors.lastname: Hall
authors.affiliation: CSIRO Biosecurity Flagship, ACT, Australia.
authors.forename: June
authors.initials: J
authors.lastname: Liu
authors.affiliation: CSIRO Biosecurity Flagship, ACT, Australia.
authors.forename: Markus
authors.initials: M
authors.lastname: Matthaei
authors.affiliation: CSIRO Biosecurity Flagship, ACT, Australia; Invasive Animals Cooperative Research Centre, University of Canberra, ACT, Australia; Institute for Applied Ecology, University of Canberra, ACT, Australia. Electronic address: tanja.strive@csiro.au.
authors.forename: Tanja
authors.initials: T
authors.lastname: Strive
mesh_terms: D000818:Animals; D002460:Cell Line; D006056:Golgi Apparatus / Q000378:metabolism / Q000821:virology; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000502:physiology*; D054884:Host-Pathogen Interactions; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020815:Polyproteins; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Calicivirus; Calicivirus polymerase; Golgi; Non-structural proteins; Oligomerisation; Polyprotein processing; RNA virus replication; Rabbit haemorrhagic disease virus; Replication