title: Identification and functional analysis of a cytochrome P450 gene CYP9AQ2 involved in deltamethrin detoxification from Locusta migratoria.
abstract: A 1578-bp cDNA of a cytochrome P450 gene (CYP9AQ2) was sequenced from the migratory locust, Locusta migratoria. It contains an open reading frame (ORF) of 1557 bp that encodes 519 amino acid residues. As compared with other known insect cytochrome P450 enzymes, the overall structure of its deduced protein is highly conserved. The expression of CYP9AQ2 was relatively higher in nymphal stages than in egg and adult stages, and the highest expression was found in fourth-instar nymphs, which was 8.7-fold higher than that of eggs. High expression of CYP9AQ2 was observed in foregut, followed by hindgut, Malpighian tubules, brain and fat bodies, which were 75~142-fold higher than that in hemolymph. Low expression was found in midgut, gastric cecum and hemolymph. The expression of CYP9AQ2 was up-regulated by deltamethrin at the concentrations of 0.04, 0.08, and 0.12 µg/mL and the maximal up-regulation was 2.6-fold at LD10 (0.04 µg/mL). RNA interference-mediated silencing of CYP9AQ2 led to an increased mortality of 25.3% when the nymphs were exposed to deltamethrin, suggesting that CYP9AQ2 plays an important role in deltamethrin detoxification in L. migratoria. Computational docking studies suggested that hydroxylation of the phenoxybenzyl moiety might be one of the deltamethrin metabolic pathways by CYP9AQ2.
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Yanqiong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Xueyao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Yu
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Jianzhen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA. Electronic address: kzhu@ksu.edu.
authors.forename: Kun Yan
authors.initials: KY
authors.lastname: Zhu
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: maenbo2003@sxu.edu.cn.
authors.forename: Enbo
authors.initials: E
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008658:Inactivation, Metabolic / Q000235:genetics*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000493:pharmacokinetics; D048188:Locusta migratoria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D009570:Nitriles / Q000493:pharmacokinetics*; D009928:Organ Specificity; D011722:Pyrethrins / Q000493:pharmacokinetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System
keywords: Cytochrome P450; Deltamethrin; Insecticide; Locusta migratoria; RNA interference