title: Secretome Identifies Tenascin-X as a Potent Marker of Ovarian Cancer.
abstract: CA-125 has been a valuable marker for the follow-up of ovarian cancer patients but it is not sensitive enough to be used as diagnostic marker. We had already used secretomic methods to identify proteins differentially secreted by serous ovarian cancer cells compared to healthy ovarian cells. Here, we evaluated the secretion of these proteins by ovarian cancer cells during the follow-up of one patient. Proteins that correlated with CA-125 levels were screened using serum samples from ovarian cancer patients as well as benign and healthy controls. Tenascin-X secretion was shown to correlate with CA-125 value in the initial case study. The immunohistochemical detection of increased amount of tenascin-X in ovarian cancer tissues compared to healthy tissues confirms the potent interest in tenascin-X as marker. We then quantified the tenascin-X level in serum of patients and identified tenascin-X as potent marker for ovarian cancer, showing that secretomic analysis is suitable for the identification of protein biomarkers when combined with protein immunoassay. Using this method, we determined tenascin-X as a new potent marker for serous ovarian cancer.
authors.affiliation: Department of Gynaecology Obstetrics, Faculty of Medicine, 1211 Geneva 14, Switzerland.
authors.forename: Marianne
authors.initials: M
authors.lastname: Kramer
authors.affiliation: Department of Gynaecology Obstetrics, Faculty of Medicine, 1211 Geneva 14, Switzerland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pierredon
authors.affiliation: Department of Gynaecology Obstetrics, Faculty of Medicine, 1211 Geneva 14, Switzerland.
authors.forename: Pascale
authors.initials: P
authors.lastname: Ribaux
authors.affiliation: Division of Clinical Pathology, HUG, 1211 Geneva 14, Switzerland.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Tille
authors.affiliation: Department of Gynaecology Obstetrics, Faculty of Medicine, 1211 Geneva 14, Switzerland.
authors.forename: Patrick
authors.identifier: 0000-0002-6835-533X
authors.initials: P
authors.lastname: Petignat
authors.affiliation: Department of Gynaecology Obstetrics, Faculty of Medicine, 1211 Geneva 14, Switzerland.
authors.forename: Marie
authors.initials: M
authors.lastname: Cohen
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000097:blood*; D019063:Tenascin / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D019063:Tenascin; C081865:tenascin X