title: New polyhydroxylated sterols from Palythoa tuberculosa and their apoptotic activity in cancer cells.
abstract: The chemical study on the total extract of the zoanthid Palythoa tuberculosa, collected from the Red Sea, resulted in the isolation of seven polyhydroxylated sterols (1-7), six of which, palysterols A-F (2-7), are new. Their chemical structures were elucidated on the basis of extensive analysis of their 1-, 2D NMR and MS spectroscopic data. This is the first chemical investigation on the species collected from Red Sea. We studied the cytotoxic effects of the total extract and some of the new polyhydroxylated sterols in three human cancer cell lines (MCF-7, HeLa, and HT-29) and one non-cancerous human cell line (KMST-6). Palysterol F (7), in particular, was able to selectively induce high levels of apoptosis (>75%) in breast adenocarcinoma (MCF-7) cells but not HeLa, HT-29 and KMST-6 cells.
authors.affiliation: Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
authors.forename: Abdulrahman M
authors.initials: AM
authors.lastname: Elbagory
authors.affiliation: Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
authors.forename: Mervin
authors.initials: M
authors.lastname: Meyer
authors.affiliation: National Institute of Oceanography and Fisheries, Attaka P.O. Box, 182, Suez, Egypt.
authors.forename: Abdel-Hamid A M
authors.initials: AH
authors.lastname: Ali
authors.affiliation: Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
authors.forename: Farouk
authors.initials: F
authors.lastname: Ameer
authors.affiliation: Zoology Department, Nelson Mandela Metropolitan University, P.O. Box 77000, Port Elizabeth 6031, South Africa.
authors.forename: Shirley
authors.initials: S
authors.lastname: Parker-Nance
authors.affiliation: Centro de Química Orgánica "Lora-Tamayo" (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Benito
authors.affiliation: Centro de Química Orgánica "Lora-Tamayo" (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Elisa Garcia
authors.initials: EG
authors.lastname: Doyagüez
authors.affiliation: Centro de Química Orgánica "Lora-Tamayo" (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Jimeno
authors.affiliation: Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa. Electronic address: ahmohammed@uwc.ac.za.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Hussein
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D006900:Hydroxylation; D010840:Phytosterols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010840:Phytosterols
keywords: Apoptosis; Marine natural products; Palysterols; Palythoa tuberculosa; Polyhydroxylated sterols; Red Sea