title: Rahnella sp. strain EK12: Cell surface properties and diesel oil biodegradation after long-term contact with natural surfactants and diesel oil.
abstract: The changes in cell surface properties of Rahnella sp. strain EK12 and modifications in genetic material after long-term contact with saponins and rhamnolipids, were investigated. Rhamnolipids caused a decrease of hydrophobicity in liquid cultures compared with saponins. On the other hand, in cultures with rhamnolipids, the addition of diesel oil results in a rapid rise of cell surface hydrophobicity. The similar effect was not so significant in the presence of saponins. For the bacteria grown in the presence of saponins or rhamnolipids, but without diesel oil, the ratio of unsaturated to saturated fatty acids decreased, in comparison to the control culture. The differences observed in hydrophobicity, zeta potential and fatty acids profiles, indicated various mechanisms of an interaction between a surfactant and a bacterial cells. The results have also shown an impact of the long-term contact on changes in genetic material of Rahnella sp. strain EK12 cells. Moreover, the presence of saponins led to significant increase of diesel oil biodegradation.
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Smułek
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Zdarta
authors.affiliation: University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Guzik
authors.affiliation: Hipolit Cegielski State College of Higher Education in Gniezno, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Dudzińska-Bajorek
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland. Electronic address: ewa.kaczorek@put.poznan.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kaczorek
mesh_terms: D001711:Biotransformation; D002462:Cell Membrane / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D005742:Gasoline; D006017:Glycolipids / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D009821:Oils / Q000378:metabolism*; D020638:Rahnella / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism; D013499:Surface Properties; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005742:Gasoline; D006017:Glycolipids; D009821:Oils; D012503:Saponins; D013501:Surface-Active Agents; C418382:rhamnolipid
keywords: Cell surface; Fatty acid; Rhamnolipids; Saponins; Zeta potential