title: A fourier transform infrared spectroscopy (FTIR) based assay for Candida parapsilosis ATCC 7330 mediated oxidation of aryl alcohols.
abstract: We present an FTIR based assay to monitor the whole cell mediated oxidation of aryl alcohols by measuring the characteristic IR absorption of the hydroxyl group [OH] of the substrate and the carbonyl group [CO] of the corresponding oxidized product. This method expedites the analysis of whole cell mediated catalysis which is usually done by GC and/or HPLC. The FTIR assay had linearity with R(2)≥0.980 and sensitivity up to 10μM. The accuracy and precision of FTIR assay was found ≥81% and ≥94%, respectively. This assay was validated by GC which exhibited ≥82% accuracy and ≥79% precision. The time of analysis taken by this assay was 2-3min per sample in comparison with 20-40min by GC.
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Biotechnology.
authors.forename: Sneha
authors.initials: S
authors.lastname: Sudhakara
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Biotechnology; National Center for Catalysis Research, Indian Institute of Technology Madras, Chennai 600 036, India. Electronic address: anjuc@iitm.ac.in.
authors.forename: Anju
authors.initials: A
authors.lastname: Chadha
mesh_terms: D000438:Alcohols / Q000032:analysis*; D002175:Candida / Q000254:growth & development* / Q000378:metabolism; D002384:Catalysis; D002849:Chromatography, Gas; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D000438:Alcohols
keywords: Biocatalytic oxidation; Candida parapsilosis ATCC 7330; FTIR spectroscopy; Gas chromatography