title: Characterization of the binding of shikonin to human immunoglobulin using scanning electron microscope, molecular modeling and multi-spectroscopic methods.
abstract: Shikonin, one of the active components isolated from the root of Arnebia euchroma (Royle) Johnst, have anti-tumor, anti-bacterial and anti-inflammatory activities and has been used clinically in phlebitis and vascular purpura. In the present work, the interaction of human immunoglobulin (HIg) with shikonin has been investigated by using scanning electron microscope (SEM), Fourier transform infrared (FT-IR) spectroscopy, fluorescence polarization, synchronous and 3D fluorescence spectroscopy in combination with molecular modeling techniques under physiological conditions with drug concentrations of 3.33-36.67 μM. The results of SEM exhibited visually the special effect on aggregation behavior of the complex formed between HIg and shikonin. The fluorescence polarization values indicated that shikonin molecules were found in a motionally unrestricted environment introduced by HIg. Molecular docking showed the shikonin moiety bound to the hydrophobic cavity of HIg, and there are four hydrogen-bonding interactions between shikonin and the residues of protein. The synchronous and 3D fluorescence spectra confirmed that shikonin could quench the intrinsic fluorescence of HIg and has an effect on the microenvironment around HIg in aqueous solution. The changes in the secondary structure of HIg were estimated by qualitative and quantitative FT-IR spectroscopic analysis. The binding constants and thermodynamic parameters for shikonin-HIg systems were obtained under different temperatures (300 K, 310 K and 320 K). The above results revealed the binding mechanism of shikonin and HIg at the ultrastructure and molecular level.
authors.affiliation: College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China. Electronic address: hwylsl-0706@163.com.
authors.forename: Wenying
authors.initials: W
authors.lastname: He
authors.affiliation: College of Chemical and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Ye
authors.affiliation: College of Chemical and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
authors.affiliation: College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Guolei
authors.initials: G
authors.lastname: Huang
authors.affiliation: College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Hua
authors.affiliation: College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hui
mesh_terms: D001665:Binding Sites; D005453:Fluorescence; D006801:Humans; D006860:Hydrogen Bonding; D007136:Immunoglobulins / Q000737:chemistry* / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D009285:Naphthoquinones / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D007136:Immunoglobulins; D009285:Naphthoquinones; C016101:shikonin
keywords: Human immunoglobulin; Interaction; Molecular modeling; Scanning electron microscope; Shikonin; Spectroscopy