title: ANXA1Ac₂₋₂₆ peptide reduces ID1 expression in cervical carcinoma cultures.
abstract: Cervical cancer is the second most frequent cancer in women worldwide and is associated with genetic alterations, infection with human papilloma virus (HPV), angiogenesis and inflammatory processes. The idea that inflammation is involved in tumorigenesis is supported by the frequent appearance of cancer in areas of chronic inflammation. On the other hand, the inflammatory response is controlled by the action of anti-inflammatory mediators, among these mediators, annexin A1 (ANXA1), a 37 kDa protein was detected as a modulator of inflammatory processes and is expressed by tumor cells. The study was carried out on the epithelial cancer cell line (SiHa) treated with the peptide of annexin A1 (ANXA1Ac2-26). We combined subtraction hybridization approach, Ingenuity Systems software and quantitative PCR, in order to evaluate gene expression influenced by ANXA1. We observed that ANXA1Ac2-26 inhibited proliferation in SiHa cells after 72h. In these cells, 55 genes exhibited changes in expression levels in response to peptide treatment. Six genes were selected and the expression results of 5 up-regulated genes (TPT1, LDHA, NCOA3, HIF1A, RAB13) and one down-regulated gene (ID1) were research by real time quantitative PCR. Four more genes (BMP4, BMPR1B, SMAD1 and SMAD4) of the ID1 pathway were investigated and only one (BMPR1B) shows the same down regulation. The data indicate the involvement of ANXA1Ac2-26 in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of cervical cancer.
authors.affiliation: Department of Biology, Institute of Biosciences, Letters and Science - IBILCE/UNESP, São José do Rio Preto, SP, Brazil.
authors.forename: Janesly
authors.initials: J
authors.lastname: Prates
authors.affiliation: Department of Biology, Institute of Biosciences, Letters and Science - IBILCE/UNESP, São José do Rio Preto, SP, Brazil.
authors.forename: Gabriela Bueno
authors.initials: GB
authors.lastname: Franco-Salla
authors.affiliation: Department of Clinical Medical, Foundation Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo - FCFRP/USP, Ribeirão Preto, SP, Brazil.
authors.forename: Anemari Ramos
authors.initials: AR
authors.lastname: Dinarte Dos Santos
authors.affiliation: Department of Clinical Medical, Foundation Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo - FCFRP/USP, Ribeirão Preto, SP, Brazil.
authors.forename: Wilson Araújo
authors.initials: WA
authors.lastname: da Silva
authors.affiliation: Department of Molecular, Biology Faculty of Medicine of São José do Rio Preto - FAMERP, São José do Rio Preto, SP, Brazil.
authors.forename: Bianca Rodrigues
authors.initials: BR
authors.lastname: da Cunha
authors.affiliation: Department of Molecular, Biology Faculty of Medicine of São José do Rio Preto - FAMERP, São José do Rio Preto, SP, Brazil.
authors.forename: Eloiza Helena
authors.initials: EH
authors.lastname: Tajara
authors.affiliation: Department of Biology, Institute of Biosciences, Letters and Science - IBILCE/UNESP, São José do Rio Preto, SP, Brazil.
authors.forename: Sonia Maria
authors.initials: SM
authors.lastname: Oliani
authors.affiliation: Department of Biology and Animal Science, Faculty of Engineering of Ilha Solteira - FEIS/UNESP, Ilha Solteira, SP, Brazil. Electronic address: flavialisoni@hotmail.com.
authors.forename: Flávia Cristina
authors.initials: FC
authors.lastname: Rodrigues-Lisoni
mesh_terms: D017305:Annexin A1 / Q000502:physiology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D000090324:Tumor Protein, Translationally-Controlled 1; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D017305:Annexin A1; D017931:DNA Primers; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1
keywords: Cell culture; Cervical cancer; Differential gene expression; Quantitative PCR; Subtractive hybridization