title: Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?
abstract: BACKGROUND
  Cancer development involves an "injury" to the respiratory machinery (Warburg effect) due to decreased or impaired mitochondrial function. This circumstance results in a down regulation of some of the ATPase subunits of the malignant tissue. The objective of this work was to assess and compare the relative expression of mRNA of mitochondrial ATPase subunits between samples of thyroid cancer and benign nodules.
  
  
  METHODS
  Samples from 31 patients who had an operation for PTC at the General Hospital of Mexico were snap-frozen and stored at -70°C. Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).
  
  
  RESULTS
  ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).
  
  
  CONCLUSIONS
  Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis. This transcript alteration may be used as a tumoral marker in papillary thyroid cancer.
authors.affiliation: Thyroid Clinic, Hospital General de Mexico, Instituto Politecnico Nacional, Mexico, Mexico.
authors.forename: Luis Mauricio
authors.initials: LM
authors.lastname: Hurtado-López
authors.affiliation: Genetic Unit, Hospital General de Mexico, Mexico, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Fernández-Ramírez
authors.affiliation: Genetic Unit, Hospital General de Mexico, Mexico, Mexico.
authors.forename: Eva
authors.initials: E
authors.lastname: Martínez-Peñafiel
authors.affiliation: Thyroid Clinic, Hospital General de Mexico, Instituto Politecnico Nacional, Mexico, Mexico.
authors.forename: José Damián
authors.initials: JD
authors.lastname: Carrillo Ruiz
authors.affiliation: Molecular Oncology, Escuela Superior de Medicina, Instituto Politecnico Nacional, Mexico, Mexico.
authors.forename: Norma Estela
authors.initials: NE
authors.lastname: Herrera González
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis / Q000235:genetics*; D011446:Prospective Studies; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D055815:Young Adult; D000097795:ATPase Inhibitory Protein
chemical_list: D014408:Biomarkers, Tumor; D025261:Mitochondrial Proton-Translocating ATPases; D011506:Proteins; D012333:RNA, Messenger; D000097795:ATPase Inhibitory Protein