title: A naturally monomeric infrared fluorescent protein for protein labeling in vivo.
abstract: Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
authors.affiliation: 1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Dan
authors.identifier: 0000000223176696
authors.initials: D
authors.lastname: Yu
authors.affiliation: 1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Baird
authors.affiliation: 1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Allen
authors.affiliation: 1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Howe
authors.affiliation: 1] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Physiology, University of California, San Francisco, San Francisco, California, USA. [3] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Klassen
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Reade
authors.affiliation: 1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Makhijani
authors.affiliation: 1] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Physiology, University of California, San Francisco, San Francisco, California, USA. [3] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Yuanquan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Murthy
authors.affiliation: 1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [3] Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Zhang
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Orion D
authors.initials: OD
authors.lastname: Weiner
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Kornberg
authors.affiliation: 1] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Physiology, University of California, San Francisco, San Francisco, California, USA. [3] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Yuh-Nung
authors.initials: YN
authors.lastname: Jan
authors.affiliation: 1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Davidson
authors.affiliation: 1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Shu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000737:chemistry; D015509:Developmental Biology; D004331:Drosophila melanogaster; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007259:Infrared Rays; D008164:Luminescent Proteins / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D014162:Transfection; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C033223:polyhistidine; D006639:Histidine; D004247:DNA