title: [Protective Effect of Total Flavonoids of Epimedium on D-galactose-induced Senescence in H9c2 Cells].
abstract: OBJECTIVE
  To investigate the protective effect of Total Flavonids of Epimedium (TFE) on D-galactose-induced H9c2 cells senescence and to explore the underlying mechanisms.
  
  
  METHODS
  D-galactosed (50 mmol/L) were used to induce the aging of H9c2 cells. Different concentrations of TFE (5,25 and 50 µg/mL) were added into the medium before the use of D-galactosed. Cells senescence was identified by β-galactosidase and the changes in cell morphology were observed. The activity of superoxide dismutase(SOD) and the content of malondial dehyde (MDA) were measured. Reactive oxygen species( ROS) was observed by DCFH-DA detection and the apoptosis was tested by Hochest.
  
  
  RESULTS
  In the D-gal group,the number of β-galactosidase-positive cells, the content of MDA and the fluorescence intensity of ROS were markedly increased. In the meanwhile,the chromatin was condensated and the apoptotic bodies were seen by using Hochest. Compared with the D-gal group, TFE apparently decreased the quantity of β-galactosidase-positive cells, the content of MDA, and the florescence intensity of ROS, increased the activity of SOD, and enhanced the condition of chromatin.
  
  
  CONCLUSIONS
  TFE can confront D-galactose-induced senescence in H9c2 cells by increasing the ability of antioxidant and reducing the number of apoptosis.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Chang-cheng
authors.initials: CC
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.forename: Yao-yan
authors.initials: YY
authors.lastname: Dun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D005690:Galactose; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005690:Galactose