title: Quantitative Comparison of Retinal Capillary Images Derived By Speckle Variance Optical Coherence Tomography With Histology.
abstract: OBJECTIVE
  The purpose of this study was to correlate human retinal capillary network information derived from a prototype speckle variance optical coherence tomography (svOCT) device with histology to determine the utility of this instrument for quantitative angiography.
  
  
  METHODS
  A retina location 3 mm superior to the optic disk was imaged with svOCT in 14 healthy human eyes. Qualitative and quantitative features of capillary networks, including capillary diameter and density, were compared with perfusion-labeled histological specimens from the same eccentricity. Twelve human donor eyes with no history of eye disease were used for histological comparisons.
  
  
  RESULTS
  svOCT was able to clearly distinguish the morphological features of the nerve fiber layer capillary network, the retinal ganglion cell (RGC) layer capillary network, the capillary network at the border of the inner plexiform layer and superficial boundary of the inner nuclear layer, and the capillary network at the boundary of the deep inner nuclear layer and outer plexiform layer. The morphological features of these networks were highly comparable to those in previous histological studies. There were no statistical differences in mean capillary diameter between svOCT images and histology for all networks other than the RGC capillary network. Capillary density measurements were significantly greater in svOCT images, except in the RGC capillary network.
  
  
  CONCLUSIONS
  svOCT has the capacity to provide histology-like anatomical information about human retinal capillary networks in vivo. It may have great potential as a research and diagnostic tool in the management of retinal vascular diseases. Further work is required to clarify the cause of some quantitative differences between svOCT and histology.
authors.affiliation: Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia 2Lions Eye Institute, University of Western Australia, Perth, Australia.
authors.forename: Priscilla Ern Zhi
authors.initials: PE
authors.lastname: Tan
authors.affiliation: Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia 2Lions Eye Institute, University of Western Australia, Perth, Australia 3Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancou.
authors.forename: Chandrakumar
authors.initials: C
authors.lastname: Balaratnasingam
authors.affiliation: School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Zaid
authors.initials: Z
authors.lastname: Mammo
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Sherry X
authors.initials: SX
authors.lastname: Han
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Mackenzie
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Kirker
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Albiani
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Merkur
authors.affiliation: School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
authors.forename: Marinko V
authors.initials: MV
authors.lastname: Sarunic
authors.affiliation: Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia 2Lions Eye Institute, University of Western Australia, Perth, Australia.
authors.forename: Dao-Yi
authors.initials: DY
authors.lastname: Yu
mesh_terms: D000328:Adult; D002196:Capillaries / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D008875:Middle Aged; D011859:Radiography; D012160:Retina / Q000033:anatomy & histology*; D012165:Retinal Ganglion Cells / Q000000981:diagnostic imaging; D012171:Retinal Vessels / Q000000981:diagnostic imaging*; D041623:Tomography, Optical Coherence / Q000379:methods* / Q000592:standards