title: Specific activation, signalling and secretion profiles of human platelets following PAR-1 and PAR-4 stimulation.
abstract: Blood platelets play a central haemostatic function; however, they also play a role in inflammation and are capable of secreting various cytokines, chemokines and related products. The purpose of this study was to identify subtle variations in platelet physiology using proteomics. We compared the levels of membrane proteins (n = 3), α and δ granule proteins (n = 18), and signalling proteins (n = 30) from unstimulated platelets with those of protease-activated receptor (PAR)-1- and PAR-4-stimulated platelets (n = 10). The vast majority of these proteins responded similarly to PAR-1 or PAR-4 engagement. However, differences were observed within membrane CD40L expressed, and α granule GRO-α and MDC secreted proteins.
authors.affiliation: a Université de Lyon , Saint Etienne , France .
authors.forename: Kim Anh
authors.initials: KA
authors.lastname: Nguyen
authors.affiliation: a Université de Lyon , Saint Etienne , France .
authors.forename: Hind
authors.initials: H
authors.lastname: Hamzeh-Cognasse
authors.affiliation: a Université de Lyon , Saint Etienne , France .|b Etablissement Français du Sang - Auvergne-Loire , Saint-Etienne , France , and.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Laradi
authors.affiliation: a Université de Lyon , Saint Etienne , France .
authors.forename: Bruno
authors.initials: B
authors.lastname: Pozzetto
authors.affiliation: a Université de Lyon , Saint Etienne , France .|c INTS - Institut National de la Transfusion Sanguine , Paris , France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Garraud
authors.affiliation: a Université de Lyon , Saint Etienne , France .|b Etablissement Français du Sang - Auvergne-Loire , Saint-Etienne , France , and.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Cognasse
mesh_terms: D015415:Biomarkers; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects; D044463:Receptor, PAR-1 / Q000819:agonists* / Q000378:metabolism; D018179:Receptors, Thrombin / Q000819:agonists* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D015415:Biomarkers; D044463:Receptor, PAR-1; D018179:Receptors, Thrombin; C112908:protease-activated receptor 4
keywords: Immunologic factors; inflammation; platelet; protease-activated receptors; signal transduction