title: Characterization of vacuolar amino acid transporter from Fusarium oxysporum in Saccharomyces cerevisiae.
abstract: Fusarium oxysporum causes wilt disease in many plant families, and many genes are involved in its development or growth in host plants. A recent study revealed that vacuolar amino acid transporters play an important role in spore formation in Schizosaccharomyces pombe and Saccharomyces cerevisiae. To investigate the role of vacuolar amino acid transporters of this phytopathogenic fungus, the FOXG_11334 (FoAVT3) gene from F. oxysporum was isolated and its function was characterized. Transcription of FoAVT3 was upregulated after rapamycin treatment. A green fluorescent protein fusion of FoAvt3p was localized to vacuolar membranes in both S. cerevisiae and F. oxysporum. Analysis of the amino acid content of the vacuolar fraction and amino acid transport activities using vacuolar membrane vesicles from S. cerevisiae cells heterologously expressing FoAVT3 revealed that FoAvt3p functions as a vacuolar amino acid transporter, exporting neutral amino acids. We conclude that the FoAVT3 gene encodes a vacuolar neutral amino acid transporter.
authors.affiliation: a Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Lunprom
authors.affiliation: a Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Pongsanat
authors.initials: P
authors.lastname: Pongcharoen
authors.affiliation: a Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.affiliation: a Faculty of Agriculture , Ehime University , Matsuyama , Japan.|b Advanced Research Support Center (ADRES) , Ehime University , Matsuyama , Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.affiliation: a Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
authors.affiliation: a Faculty of Agriculture , Ehime University , Matsuyama , Japan.|b Advanced Research Support Center (ADRES) , Ehime University , Matsuyama , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000166:cytology* / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D005656:Fungal Proteins
keywords: FoAVT3; Fusarium oxysporum; neutral amino acid transport; vacuolar amino acid transporter