title: Differentiation of bovine satellite cell-derived myoblasts under different culture conditions.
abstract: The aim of this study was to develop adequate in vitro conditions for the differentiation of bovine skeletal muscle cells. Therefore, satellite cells isolated from the left foreleg of a Holstein-Friesian fetus at 4.5 mo of gestation were seeded on 24-well plates coated with extracellular matrix gel. Cells were cultured for 5 d in growth medium containing 10% fetal bovine serum. After reaching confluence, several differentiation media were tested for inducing myotube formation. The highest fusion rate of approximately 30% was achieved with a serum-free medium containing 1 μM dexamethasone, 1 μg/ml linoleic acid, and 0.1 μM insulin after a differentiation phase of 72 h. Two different culture conditions (serum-free and serum-containing) appropriate for bovine skeletal muscle cell differentiation are described in detail which allow the investigation of bovine skeletal muscle cell proliferation and differentiation in general as well as in response to bioactive compounds.
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. will@fbn-dummerstorf.de.
authors.forename: Katja
authors.initials: K
authors.lastname: Will
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Schering
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Albrecht
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Kalbe
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Maak
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D018482:Muscle, Skeletal / Q000166:cytology* / Q000196:embryology; D032446:Myoblasts / Q000166:cytology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*
keywords: Bovine satellite cells; Differentiation; Myoblasts; Serum-free medium