title: Effects of ferulic acid on antioxidant activity in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and their combination.
abstract: The present study aimed at exploring different roles of the same compound in different environment, using preparative HPLC, and the significance to investigating bio-active constituents in traditional Chinese medicine (TCM) on the basis of holism. In this study, the depletion of target component ferulic acid (FA) by using preparative HPLC followed by antioxidant activity testing was applied to investigate the roles of FA in Angelicae Sinensis Radix (DG), Chuanxiong Rhizoma (CX) and their combination (GX). The antioxidant activity was performed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity testing. FA was successfully and exclusively depleted from DG, CX, and GX, respectively. By comparing the effects of the samples, it was found that FA was one of the main antioxidant constituents in DG, CX and GX, and the roles of FA were DG > CX > GX. Furthermore, the effects of FA varied at different doses in these herbs. This study provided a reliable and effective approach to clarifying the contribution of same compound in different TCMs to their bio-activities. The role of a constituent in different TCMs might be different, and a component with the same content might have different effects in different chemical environments. Furthermore, this study also suggested the potential utilization of preparative HPLC in the characterization of the roles of multi-ingredients in TCM.
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Lin-Yan
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China. Electronic address: yupingtang@njucm.edu.cn.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Tao
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China.
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Li
authors.affiliation: Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China.
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C004999:ferulic acid
keywords: Angelica sinensis; Antioxidant activity; Ferulic acid; HPLC; Herb pair; Ligusticum chuanxiong