title: Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.
abstract: Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. To achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 was used to label the cancer cells of a pancreatic cancer PDOX. The PDOX was previously grown in a red fluorescent protein (RFP) transgenic mouse that stably labeled the PDOX stroma cells bright red. The color-coded PDOX model enabled FGS to completely resect the pancreatic tumors including stroma. Dual-colored FGS significantly prevented local recurrence, which bright-light surgery or single-color FGS could not. FGS, with color-coded cancer and stroma cells has important potential for improving the outcome of recalcitrant-cancer surgery.
authors.affiliation: 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA [3] Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yano
authors.affiliation: 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Hiroshima
authors.affiliation: Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: A
authors.initials: A
authors.lastname: Maawy
authors.affiliation: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kishimoto
authors.affiliation: Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Suetsugu
authors.affiliation: 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: S
authors.initials: S
authors.lastname: Miwa
authors.affiliation: 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Toneri
authors.affiliation: 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Surgical Oncoloy, MD Anderson Cancer Center, University of Texas, Houston, TX, USA.
authors.forename: M H G
authors.initials: MH
authors.lastname: Katz
authors.affiliation: Department of Surgical Oncoloy, MD Anderson Cancer Center, University of Texas, Houston, TX, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Fleming
authors.affiliation: Oncolys BioPharm, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Urata
authors.affiliation: Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tazawa
authors.affiliation: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kagawa
authors.affiliation: Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.affiliation: 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008819:Mice, Nude; D008822:Mice, Transgenic; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D025321:Surgery, Computer-Assisted; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein