title: Phytochemical Profiling and Evaluation of Pharmacological Activities of Hypericum scabrum L.
abstract: Phytochemical investigations of ethyl acetate-soluble part of the aerial part of Hypericum scabrum L. delivered eight pure phenolic compounds 1-8. The pure compounds were identified through physico-chemical, NMR (1D, 2D) and mass spectrometric studies as: 3-8''-bisapigenin (1), quercetin (2), quercetin-3-O-α-l-arabinofuranoside (3), quercetin-3-O-α-l-rhamnoside (4), quercetin-3-O-β-d-glucopyranoside (5), quercetin-3-O-β-d-galactopyranoside (6), (-)-epicatechin (7), (+)-catechin (8). Total polyphenolic compounds and total flavonoids contents were determined in the extract as 0.107 mg∙mg-1 and 0.023 mg∙mg-1 of the dried extract, respectively. Antioxidant activity using DPPH free radical scavenging assay delivered very strong activity for compounds 2 and 5, 6 and crude extract 10. Protein tyrosine phosphatase 1B (PTP-1B) inhibition experiment of isolated compounds and crude extracts resulted in significant inhibition activity for samples 2, 7a, 8a, 11 and 12 with IC50 values ranging from 1.57 to 2.91 µM. Antimicrobial activity of the pure compounds and extracts produced average results against Staphylococcus aureus, Escherichia coli and Candida albicans strains. From our literature survey, it appears that all pure compounds except 2 were isolated and reported for the first time in H. scabrum.
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. jianglan@ms.xjb.ac.cn.|Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. jianglan@ms.xjb.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. jianglan@ms.xjb.ac.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. sodikjon82@gmail.com.|Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. sodikjon82@gmail.com.|State Scientifically-Experimental and Production Organization, Academy of Sciences of the Republic of Tajikistan, Dushanbe 734063, Tajikistan. sodikjon82@gmail.com.
authors.forename: Sodik
authors.initials: S
authors.lastname: Numonov
authors.affiliation: Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 100170 Tashkent, Uzbekistan. khayrulla@rambler.ru.
authors.forename: Khayrulla
authors.initials: K
authors.lastname: Bobakulov
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. mnasimuq@yahoo.com.|Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. mnasimuq@yahoo.com.|Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan. mnasimuq@yahoo.com.
authors.forename: Muhammad Nasimullah
authors.initials: MN
authors.lastname: Qureshi
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. haiqing_zhq@126.com.|Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. haiqing_zhq@126.com.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. haji@ms.xjb.ac.cn.|Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. haji@ms.xjb.ac.cn.
authors.forename: Haji Akber
authors.initials: HA
authors.lastname: Aisa
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D020902:Hypericum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D064209:Phytochemicals / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1
keywords: ESI-MS; Hypericum scabrum L.; NMR; antidiabetic activity; antimicrobial activity; antioxidant activity; column chromatography; ethylacetate fraction