title: A new approach to detection of incomplete antibodies using hydrogel chromatography medium.
abstract: In assays for incomplete antibody detection, several washing steps are required to remove unbound globulins which may cause false negatives. Here, we present an improved approach employing hydrogel chromatography medium (HCM) in the detection of incomplete antibodies. After a rapid single-step centrifugation, incomplete antibodies, attached to red blood cells (RBCs), were separated from the reaction mixture using HCM and sedimentation. This method obviates the need for multiple centrifugation steps found in conventional Tube-Coombs tests. The HCM-Coombs tests may have a wide range of applications for incomplete antibody detection.
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China. Electronic address: wanghm449@aliyun.com.
authors.forename: HongMei
authors.initials: H
authors.lastname: Wang
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: YeZhou
authors.initials: Y
authors.lastname: Chen
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: ShaoHua
authors.initials: S
authors.lastname: Ding
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: ShengBao
authors.initials: S
authors.lastname: Duan
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: JingJing
authors.initials: J
authors.lastname: Tian
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: QingLin
authors.initials: Q
authors.lastname: Meng
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: ShuangShi
authors.initials: S
authors.lastname: Wei
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88, Keling Road, Suzhou New District, Jiangsu Province 215163, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000097:blood*; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male
chemical_list: D000906:Antibodies; D020100:Hydrogels
keywords: Coombs tests; Hydrogel chromatography medium; Immunoassay; Incomplete antibody