title: Dimerization of TRAF-interacting protein (TRAIP) regulates the mitotic progression.
abstract: The homo- or hetero-dimerization of proteins plays critical roles in the mitotic progression. The TRAF-interacting protein (TRAIP) is crucial in early mitotic progression and chromosome alignment defects in the metaphase. The TRAIP is a 469 amino acid protein, including the Really Interesting New Gene (RING), coiled-coil (CC), and leucine zipper (LZ) domain. In general, the CC or LZ domain containing proteins forms homo- or hetero-dimerization to achieve its activity. In this study, a number of TRAIP mutants were used to define the TRAIP molecular domains responsible for its homo-dimerization. A co-immunoprecipitation assay indicated that the TRAIP forms homo-dimerization through the CC domain. The cells, expressing the CC domain-deleted mutant that could not form a homo-dimer, increased the mitotic index and promoted mitotic progression.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
authors.forename: I Seul
authors.initials: IS
authors.lastname: Park
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Ku-Sung
authors.initials: KS
authors.lastname: Jo
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea. Electronic address: wonhs@kku.ac.kr.
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Won
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea. Electronic address: khtcat@skku.edu.
authors.forename: Hongtae
authors.initials: H
authors.lastname: Kim
mesh_terms: D019281:Dimerization; D005455:Fluorescent Antibody Technique; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / Q000378:metabolism* / Q000502:physiology
chemical_list: D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
keywords: Coiled-coil domain; Dimerization; Mitotic progression; TRAIP