title: The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm.
abstract: Centrobin resides in daughter centriole and play a critical role in centriole duplication. Nucleation and stabilization of microtubules are known biological activities of centrobin. Here, we report a specific localization of centrobin outside the centrosome. Centrobin was associated with the stable microtubules. In hippocampal cells, centrobin formed cytoplasmic dots in addition to the localization at both centrosomes with the mother and daughter centrioles. Such specific localization pattern suggests that cytoplasmic centrobin is not just a reserved pool for centrosomal localization but also has a specific role in the cytoplasm. In fact, centrobin enhanced microtubule formation outside as well as inside the centrosome. These results propose specific roles of the cytoplasmic centrobin for noncentrosomal microtubule formation in specific cell types and during the cell cycle.
authors.affiliation: a Department of Biological Sciences; Seoul National University ; Seoul , Korea.
authors.forename: Wonjung
authors.initials: W
authors.lastname: Shin
authors.forename: Nam-Kyung
authors.initials: NK
authors.lastname: Yu
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
authors.forename: Kunsoo
authors.initials: K
authors.lastname: Rhee
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000502:physiology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002502:Centrioles / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D000072379:NIMA-Related Kinases; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection
chemical_list: C507203:CNTROB protein, human; D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; C089441:NEK2 protein, human; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases
keywords: NEK2; centrobin; centrosome; microtubule; mouse hippocampus; nucleation