title: Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain.
abstract: Synthetic auxotrophs are organisms engineered to require the presence of a particular molecule for viability. They have potential applications in biocontainment and enzyme engineering. We show that these organisms can be generated by engineering ligand-dependence into essential genes. We demonstrate a method for generating a Synthetic auxotroph based on a Ligand-Dependent Essential gene (SLiDE) using 5 essential genes as test cases: pheS, dnaN, tyrS, metG, and adk. We show that a single SLiDE strain can have a 1 × 10(8)-fold increase in viability when chemically complemented with the ligand benzothiazole. The optimized SLiDE strain engineering protocol required less than 1 week and $100 USD. We combined multiple SLiDE strain alleles into the industrial Escherichia coli strain BL21(DE3), yielding an organism that exceeds the biosafety criteria with an escape frequency below the limit of detection of 3 × 10(-11).
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Lopez
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Anderson
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods*
keywords: biosafety; biosensor; ligand-dependent essential gene; penicillin technique; protein engineering; synthetic auxotroph