title: Predictive Potential of Twenty-Two Biochemical Biomarkers for Coronary Artery Disease in Type 2 Diabetes Mellitus.
abstract: We investigated the potential of a panel of 22 biomarkers to predict the presence of coronary artery disease (CAD) in type 2 diabetes mellitus (DM2) patients. The study enrolled 96 DM2 patients with (n = 75) and without (n = 21) evidence of CAD. We assessed a biochemical profile that included 22 biomarkers: total cholesterol, LDL, HDL, LDL/HDL, triglycerides, glucose, glycated hemoglobin, fructosamine, homocysteine, cysteine, methionine, reduced glutathione, oxidized glutathione, reduced glutathione/oxidized glutathione, L-arginine, asymmetric dimethyl-L-arginine, symmetric dimethyl-L-arginine, asymmetric dimethyl-L-arginine/L-arginine, nitrate plus nitrite, S-nitrosothiols, nitrotyrosine, and n-acetyl-β-glucosaminidase. Prediction models were built using logistic regression models. We found that eight biomarkers (methionine, nitratate plus nitrite, n-acetyl-β-glucosaminidase, BMI, LDL, HDL, reduced glutathione, and L-arginine/asymmetric dimethyl-L-arginine) along with gender and BMI were significantly associated with the odds of CAD in DM2. These preliminary findings support the notion that emerging biochemical markers might be used for CAD prediction in patients with DM2. Our findings warrant further investigation with large, well-designed studies.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil ; Universidade Federal de São Paulo, 09913-030 Diadema, SP, Brazil.
authors.forename: Edimar Cristiano
authors.initials: EC
authors.lastname: Pereira
authors.affiliation: Instituto Dante Pazzanese de Cardiologia, 04012-180 São Paulo, SP, Brazil.
authors.forename: Marcelo Chiara
authors.initials: MC
authors.lastname: Bertolami
authors.affiliation: Instituto Dante Pazzanese de Cardiologia, 04012-180 São Paulo, SP, Brazil.
authors.forename: André Arpad
authors.initials: AA
authors.lastname: Faludi
authors.affiliation: Faculdade de Ciências M'edicas, Universidade Metodista de Santos, 11045-101 Santos, SP, Brazil.
authors.forename: Osmar
authors.initials: O
authors.lastname: Monte
authors.affiliation: Santa Casa de Misericórdia de São Paulo, 01221-020 São Paulo, SP, Brazil Santa Casa de Misericórdia de São Paulo, 01221-020 São Paulo, SP, Brazil.
authors.forename: Hermes Toros
authors.initials: HT
authors.lastname: Xavier
authors.affiliation: Unidade de Avaliação de Tecnologias em Saúde, Instituto de Educação e Ciências em Saúde, Hospital Alemão Oswaldo Cruz, 01323-903 São Paulo, SP, Brazil ; Laboratório de Genética e Cardiologia Molecular, Instituto do Coração (InCor), 05403-900 São Paulo, SP, Brazil.
authors.forename: Tiago Veiga
authors.initials: TV
authors.lastname: Pereira
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil.
authors.forename: Dulcineia Saes Parra
authors.identifier: 0000-0001-9608-3135
authors.initials: DS
authors.lastname: Abdalla