title: Ionic liquid and aqueous two-phase extraction based on salting-out coupled with high-performance liquid chromatography for the determination of seven rare ginsenosides in Xue-Sai-Tong injection.
abstract: A method of ionic liquid salt aqueous two-phase extraction coupled with high-performance liquid chromatography has been developed for the analysis of seven rare ginsenosides including Rg6 , F4 , 20(S)-Rg3 , 20(R)-Rg3 , Rk3 , Rk1 , and Rg5 in Xue-Sai-Tong injection. The injection was mixed with ionic liquid 1-butyl-3-methylimidazolium bromide aqueous solution, and a mixture was obtained. With the addition of sodium dodecyl sulfate and dipotassium phosphate into the mixture, the aqueous two-phase mixture was formed after ultrasonic treatment and centrifuged. Rare ginsenosides were extracted into the upper phase. To obtain a high extraction factors, various influences were considered systematically, such as the volume of ionic liquid, the category and amount of salts, the amount of sodium dodecyl sulfate, the pH value of system, and the time of ultrasonic treatment. Under the optimal condition, rare ginsenosides in Xue-Sai-Tong injection were enriched and detected, the recoveries of seven rare ginsenosides ranged from 90.05 to 112.55%, while relative standard deviations were lower than 2.50%. The developed method was reliable, rapid and sensitive for the determination of seven rare ginsenosides in the injections.
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Lan-Jie
authors.initials: LJ
authors.lastname: Li
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Yong-Ri
authors.initials: YR
authors.lastname: Jin
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Wang
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Quartermaster Technology, Jilin University, Changchun, P. R. China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Shi
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Xu-Wen
authors.initials: XW
authors.lastname: Li
mesh_terms: D002712:Chlorides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids; D057230:Limit of Detection; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D053723:Panax notoginseng; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate; D052616:Solid Phase Extraction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D014465:Ultrasonics
chemical_list: C525963:1-butyl-3-methylimidazolium; D002712:Chlorides; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D007093:Imidazoles; D052578:Ionic Liquids; D010936:Plant Extracts; D012503:Saponins; C509799:xuesetong; D012967:Sodium Dodecyl Sulfate
keywords: Aqueous two-phase extraction; Ginsenosides; High-performance liquid chromatography; Ionic liquids; Xue-Sai-Tong injection