title: [Protective effects of 3-methyladenine on acute lung injury caused by multiple trauma in rats].
abstract: OBJECTIVE
  To investigate the role of autophagy in acute lung injury (ALI) caused by multiple trauma in rats via pretreat with 3-methyladenine (3-MA).
  
  
  METHODS
  Forty-five Sprague-Dawley male rats, with age of 4 months and body weight of 250-300 g,were randomly divided into three groups. In the sham group, the rats received sphenotresia only;in the control group, the rats were made model of femur shaft fracture combined with brain injury, and treated with physiological saline by abdominal cavity at 1 hour before making model; in the 3-MA group, the rats were made model of femur shaft fracture combined with brain injury,and treated with 3-MA of 10 mg/kg by abdominal cavity at 1 hour before making model. Histologic changes and the concentration of related inflammatory factors in the damaged lung tissue were examined at 48 h after opteration, at the same time, the effect of 3-MA on the expression of LC-3 II and Beclin-1 was examined through reverse transcriptase polymerase chain reaction technique (RT-PCR).
  
  
  RESULTS
  Compared with sham group, LC-3 II and Beclin-1 level in control group at 48 h after operation were obviously increased (P < 0.01). Compared with control group, LC-3 II and Beclin-1 level in 3-MA group at 48 h after operation were obviously decreased (P < 0.01). Compared with sham group, the level of proinflammatory cytokines (TNF-α and IL-6) in control group obviously enhanced (P < 0.01). Compared with control group, above items in 3-MA group was obviously lower (P < 0.01). Compared with control group,the histopathological damage of lung in 3-MA group obviously reduced (P < 0.01).
  
  
  CONCLUSIONS
  Autophagy can aggravate the acute lung injury caused by fracture of shaft of femur combined with brain injuries,but 3-MA can reduce tissue damage by inhibiting the autophagy and inflammatory response.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Hao
authors.forename: Long
authors.initials: L
authors.lastname: Yi
authors.forename: Tian-Sheng
authors.initials: TS
authors.lastname: Sun
mesh_terms: D055371:Acute Lung Injury / Q000517:prevention & control*; D000225:Adenine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000032:analysis; D000071186:Beclin-1; D015850:Interleukin-6 / Q000032:analysis; D008168:Lung / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D008297:Male; D009104:Multiple Trauma / Q000150:complications*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C512829:Becn1 protein, rat; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C025946:3-methyladenine; D000225:Adenine