title: Comparison of redox and gene expression changes during vegetative/generative transition in the crowns and leaves of chromosome 5A substitution lines of wheat under low-temperature condition.
abstract: The aim of our experiments was to investigate the effect of chromosome 5A on the thiol-dependent redox environment and on the transcription of cold- and vernalization-related genes during the vegetative/generative transition in crowns and leaves of wheat. Chinese Spring, a moderately freezing-tolerant variety, and its more and less tolerant substitution lines - [CS(Ch5A)] and [CS(Tsp5A)], respectively - with different combinations of vernalization alleles were compared. At low temperature, the amount of cystine and glutathione disulphide and the related redox potentials increased in the crowns but not in the leaves. In the crowns of the substitution lines, the concentration and redox state of thiols were different only at the vegetative and double ridge (start of the generative transition) stages. The expression of the vernalization-related VRN1 gene increased significantly during the transition both in the crowns and leaves. The transcription of the freezing tolerance-related CBF14, COR14b and COR39 genes markedly increased in both organs after 2 weeks at 4 °C when the seedlings were still in the vegetative stage. This increment was greater in CS(Ch5A) than in CS(Tsp5A). The Ch5A chromosome in CS genetic background enhanced the expression of CBF regulon even in the generative phase in crown that is the key organ for overwintering and freezing tolerance. At certain developmental stages, both the thiol and the transcript levels differed significantly in the two substitution lines.
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Boldizsár
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.
authors.forename: Dániel Á
authors.initials: DÁ
authors.lastname: Carrera
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.|Doctoral School of Molecular- and Nanotechnologies, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Veszprém, 8200, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Gulyás
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Vashegyi
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.|Doctoral School of Molecular- and Nanotechnologies, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Veszprém, 8200, Hungary.
authors.forename: Aliz
authors.initials: A
authors.lastname: Novák
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.|Doctoral School of Molecular- and Nanotechnologies, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Veszprém, 8200, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kalapos
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.
authors.forename: Magda
authors.initials: M
authors.lastname: Pál
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.|Doctoral School of Animal- and Agricultural Environmental Sciences, Department of Meteorology and Water Management, Georgikon Faculty, University of Pannonia, Keszthely, 8360, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Galiba
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary. kocsy.gabor@agrar.mta.hu.|Doctoral School of Molecular- and Nanotechnologies, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Veszprém, 8200, Hungary. kocsy.gabor@agrar.mta.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Kocsy
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D013438:Sulfhydryl Compounds
keywords: Chromosome 5A; Freezing tolerance; Glutathione; Redox control; Triticum aestivum; Vernalization