title: Glycoprotein 5 of porcine reproductive and respiratory syndrome virus strain SD16 inhibits viral replication and causes G2/M cell cycle arrest, but does not induce cellular apoptosis in Marc-145 cells.
abstract: Cell apoptosis is common after infection with porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV GP5 has been reported to induce cell apoptosis. To further understand the role of GP5 in PRRSV induced cell apoptosis, we established Marc-145 cell lines stably expressing full-length GP5, GP5(Δ84-96) (aa 84-96 deletion), and GP5(Δ97-119) (aa 97-119 deletion). Cell proliferation, cell cycle progression, cell apoptosis and virus replication in these cell lines were evaluated. Neither truncated nor full-length GP5 induced cell apoptosis in Marc-145 cells. However, GP5(Δ97-119), but not full-length or GP5(Δ84-96), induced a cell cycle arrest at the G2/M phase resulting in a reduction in the growth of Marc-145 cells. Additionally, GP5(Δ84-96) inhibited the replication of PRRSV in Marc-145 cells through induction of IFN-β. These findings suggest that PRRSV GP5 is not responsible for inducing cell apoptosis in Marc-145 cells under these experimental conditions; however it has other important roles in virus/host cell biology.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: muyang@nwsuaf.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: lifeiyang2007@126.com.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: diana851218@163.com.
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: hbch228@163.com.
authors.forename: Baicheng
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: jimingao2006@163.com.
authors.forename: Jiming
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: wangxiangpeng2003@126.com.
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: wangchengbao@126.com.
authors.forename: Chengbao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: shqxiaojd@hotmail.com.
authors.forename: Shuqi
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: qinzhao_2004@sina.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: yanisun_2005@sina.com.
authors.forename: Yani
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China. Electronic address: Zhanggaiping2003@yahoo.com.cn.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK. Electronic address: julian.hiscox@liverpool.ac.uk.
authors.forename: Julian A
authors.initials: JA
authors.lastname: Hiscox
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People׳s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: zhouem@nwsuaf.edu.cn.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D002460:Cell Line; D049109:Cell Proliferation; D017353:Gene Deletion; D054884:Host-Pathogen Interactions; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000502:physiology*; D013552:Swine; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: Cell apoptosis; GP5; IFN; PRRSV; Virus replication