title: Noninvasive high-speed photoacoustic tomography of cerebral hemodynamics in awake-moving rats.
abstract: We present a noninvasive method of photoacoustic tomography (PAT) for imaging cerebral hemodynamics in awake-moving rats. The wearable PAT (wPAT) system has a size of 15 mm in height and 33 mm in diameter, and a weight of ~8 g (excluding cabling). The wPAT achieved an imaging rate of 3.33 frames/s with a lateral resolution of 243 μm. Animal experiments were designed to show wPAT feasibility for imaging cerebral hemodynamics on awake-moving animals. Results showed that the cerebral oxy-hemoglobin and deoxy-hemoglobin changed significantly in response to hyperoxia; and, after the injection of pentylenetetrazol (PTZ), cerebral blood volume changed faster over time and larger in amplitude for rats in awake-moving state compared with rats under anesthesia. By providing a light-weight, high-resolution technology for in vivo monitoring of cerebral hemodynamics in awake-behaving animals, it will be possible to develop a comprehensive understanding on how activity alters hemodynamics in normal and diseased states.
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Tang
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Xi
authors.affiliation: Department of Pediatrics, University of Florida, Gainesville, Florida, USA.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhou
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: 1] J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA [2] Department of Pediatrics, University of Florida, Gainesville, Florida, USA [3] Department of Neurology, University of Florida, Gainesville, Florida, USA [4] Department of Neuroscience, University of Florida, Gainesville, Florida, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Carney
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
authors.forename: Huabei
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D018756:GABA Antagonists / Q000494:pharmacology; D018496:Hyperoxia / Q000503:physiopathology; D008124:Locomotion / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology; D061088:Photoacoustic Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology*
chemical_list: D018756:GABA Antagonists; D010433:Pentylenetetrazole