title: Shlnc-EC6 regulates murine erythroid enucleation by Rac1-PIP5K pathway.
abstract: Long noncoding RNAs (LncRNAs) are longer than 200 nucleotide noncoding RNAs without apparent functional coding capacity that function as regulators of cell growth and development. In recent years, increasing evidence implicates the involvement of LncRNAs in erythropoiesis. shlnc-EC6 is a LncRNA associated with erythroid differentiation but the mechanism remains undefined. In this study, we found that knockdown of shlnc-EC6 in purified mouse fetal liver erythroid progenitor and hematopoietic stem cells (FLEPHSCs) significantly blocked erythroid enucleation. We also showed that Rac1 was negatively regulated by shlnc-EC6 at the posttranscriptional level via specific binding to sites within the 3'UTR of Rac1 mRNA. Moreover, we found that knockdown of shlnc-EC6 led to upregulation of Rac1, followed by the activation of the downstream protein PIP5K, and subsequently resulted in the inhibition of enucleation in cultured mouse fetal erythroblasts. Thus, our findings suggest that shlnc-EC6 acts as a novel modulator to regulate mouse erythropoiesis via Rac1/PIP5K signaling pathway.
authors.affiliation: Non-coding RNA Center, Yangzhou University, 11 Huaihai Road, Yangzhou, 225001, China.
authors.forename: Chenghai
authors.initials: C
authors.lastname: Wang
authors.affiliation: Non-coding RNA Center, Yangzhou University, 11 Huaihai Road, Yangzhou, 225001, China.|Department of Pediatrics, Yangzhou University Affiliated Jingjiang Hospital, Jingjiang, 214500, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wu
authors.affiliation: Non-coding RNA Center, Yangzhou University, 11 Huaihai Road, Yangzhou, 225001, China.
authors.forename: Feiyang
authors.initials: F
authors.lastname: Shen
authors.affiliation: Non-coding RNA Center, Yangzhou University, 11 Huaihai Road, Yangzhou, 225001, China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Non-coding RNA Center, Yangzhou University, 11 Huaihai Road, Yangzhou, 225001, China.|Department of Pathology, Yangzhou University School of Medicine, 11 Huaihai Road, Yangzhou, 225001, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Non-coding RNA Center, Yangzhou University, 11 Huaihai Road, Yangzhou, 225001, China.|Department of Physiology, Yangzhou University School of Medicine, 11 Huaihai Road, Yangzhou, 225001, China.|Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, 11 Huaihai Road, Yangzhou, 225001, China.|Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, 11 Huaihai Road, Yangzhou, 225001, China.
authors.forename: Duonan
authors.initials: D
authors.lastname: Yu
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004912:Erythrocytes / Q000502:physiology; D041905:Erythroid Cells / Q000187:drug effects / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000502:physiology*; D004920:Erythropoiesis / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D062085:RNA, Long Noncoding / Q000494:pharmacology / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology / Q000502:physiology*
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering
keywords: Fetal liver erythroid progenitor; PIP5K; Rac1; Shlnc-EC6; enucleation