title: Evaluation of the Efficiency of the Sample Inactivation Reagent in the Abbott RealTime MTB Assay for Inactivation of Mycobacterium tuberculosis.
abstract: The Abbott RealTime MTB assay is a nucleic acid amplification test (NAAT) for the detection of Mycobacterium tuberculosis complex DNA. The sample inactivation procedure used in the assay, consisting of one part sample treated with 3 parts inactivation reagent for 60 min, effectively reduced viscosity and inactivated M. tuberculosis in clinical specimens.
authors.affiliation: Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA Clinical Microbiology Laboratory, Northwestern Memorial Hospital, Chicago, Illinois, USA cqi451@northwestern.edu.
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
authors.affiliation: Lancet Laboratories, Johannesburg, South Africa BARC-SA, Johannesburg, South Africa.
authors.forename: Carole
authors.initials: C
authors.lastname: Wallis
authors.affiliation: Abbott Molecular, Des Plaines, Illinois, USA.
authors.forename: Vihanga
authors.initials: V
authors.lastname: Pahalawatta
authors.affiliation: Abbott Molecular, Des Plaines, Illinois, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Frank
authors.affiliation: Lancet Laboratories, Johannesburg, South Africa BARC-SA, Johannesburg, South Africa.
authors.forename: Neeshan
authors.initials: N
authors.lastname: Ramdin
authors.affiliation: Lancet Laboratories, Johannesburg, South Africa BARC-SA, Johannesburg, South Africa.
authors.forename: Raquel
authors.initials: R
authors.lastname: Viana
authors.affiliation: Abbott Molecular, Des Plaines, Illinois, USA.
authors.forename: Klara
authors.initials: K
authors.lastname: Abravaya
authors.affiliation: Abbott Molecular, Des Plaines, Illinois, USA.
authors.forename: Gregor
authors.initials: G
authors.lastname: Leckie
authors.affiliation: Abbott Molecular, Des Plaines, Illinois, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Tang
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004203:Disinfection / Q000379:methods*; D006801:Humans; D050296:Microbial Viability; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D014783:Viscosity