title: TRIP through the chromatin: a high throughput exploration of enhancer regulatory landscapes.
abstract: Enhancers are regulatory elements that promote gene expression in a spatio-temporal way and are involved in a wide range of developmental and disease processes. Both the identification and subsequent functional dissection of enhancers are key steps in understanding these processes. Several high-throughput approaches were recently developed for these purposes; however, in almost all cases enhancers are being tested outside their native chromatin context. Until recently, the analysis of enhancer activities at their native genomic locations was low throughput, laborious and time-consuming. Here, we discuss the potential of a powerful approach, TRIP, to study the functioning of enhancers in their native chromatin environments by introducing sensor constructs directly in the genome. TRIP allows for simultaneously analyzing the quantitative readout of numerous sensor constructs integrated at random locations in the genome. The high-throughput and flexible nature of TRIP opens up potential to study different aspects of enhancer biology at an unprecedented level.
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk 630090, Russia. Electronic address: a.pindyurin@mcb.nsc.ru.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Pindyurin
authors.affiliation: Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands.
authors.forename: Johann
authors.initials: J
authors.lastname: de Jong
authors.affiliation: Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands. Electronic address: w.akhtar@nki.nl.
authors.forename: Waseem
authors.initials: W
authors.lastname: Akhtar
mesh_terms: D002843:Chromatin / Q000235:genetics*; D055012:Chromosomal Position Effects / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000235:genetics; D015894:Genome, Human; D023281:Genomics; D006801:Humans
chemical_list: D002843:Chromatin
keywords: Barcoded reporters; Chromatin; Enhancer; Gene regulation; Position effect; Sensor constructs; TRIP