title: Chondroitin Sulfate Induces Depression of Synaptic Transmission and Modulation of Neuronal Plasticity in Rat Hippocampal Slices.
abstract: It is currently known that in CNS the extracellular matrix is involved in synaptic stabilization and limitation of synaptic plasticity. However, it has been reported that the treatment with chondroitinase following injury allows the formation of new synapses and increased plasticity and functional recovery. So, we hypothesize that some components of extracellular matrix may modulate synaptic transmission. To test this hypothesis we evaluated the effects of chondroitin sulphate (CS) on excitatory synaptic transmission, cellular excitability, and neuronal plasticity using extracellular recordings in the CA1 area of rat hippocampal slices. CS caused a reversible depression of evoked field excitatory postsynaptic potentials in a concentration-dependent manner. CS also reduced the population spike amplitude evoked after orthodromic stimulation but not when the population spikes were antidromically evoked; in this last case a potentiation was observed. CS also enhanced paired-pulse facilitation and long-term potentiation. Our study provides evidence that CS, a major component of the brain perineuronal net and extracellular matrix, has a function beyond the structural one, namely, the modulation of synaptic transmission and neuronal plasticity in the hippocampus.
authors.affiliation: Teófilo Hernando Institute for Drug Discovery, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Pharmacology and Therapeutics, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Clinical Pharmacology, Instituto de Investigación Sanitaria Hospital de la Princesa, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Albiñana
authors.affiliation: Teófilo Hernando Institute for Drug Discovery, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Pharmacology and Therapeutics, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Clinical Pharmacology, Instituto de Investigación Sanitaria Hospital de la Princesa, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Gutierrez-Luengo
authors.affiliation: Teófilo Hernando Institute for Drug Discovery, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Pharmacology and Therapeutics, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Clinical Pharmacology, Instituto de Investigación Sanitaria Hospital de la Princesa, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Hernández-Juarez
authors.affiliation: Teófilo Hernando Institute for Drug Discovery, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Pharmacology and Therapeutics, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Clinical Pharmacology, Instituto de Investigación Sanitaria Hospital de la Princesa, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: Andrés M
authors.initials: AM
authors.lastname: Baraibar
authors.affiliation: Departamento de Investigación, Laboratorios Bioibérica, 08029 Barcelona, Spain.
authors.forename: Eulalia
authors.initials: E
authors.lastname: Montell
authors.affiliation: Departamento de Investigación, Laboratorios Bioibérica, 08029 Barcelona, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Vergés
authors.affiliation: Teófilo Hernando Institute for Drug Discovery, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Pharmacology and Therapeutics, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Clinical Pharmacology, Instituto de Investigación Sanitaria Hospital de la Princesa, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: Antonio G
authors.initials: AG
authors.lastname: García
authors.affiliation: Teófilo Hernando Institute for Drug Discovery, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Pharmacology and Therapeutics, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain ; Department of Clinical Pharmacology, Instituto de Investigación Sanitaria Hospital de la Princesa, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: Jesus M
authors.initials: JM
authors.lastname: Hernández-Guijo
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000502:physiology*; D019765:Chondroitin ABC Lyase / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects*; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002809:Chondroitin Sulfates; D019765:Chondroitin ABC Lyase