title: Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury.
abstract: OBJECTIVE
  To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury.
  
  
  METHODS
  36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (n = 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-rich saline treatment group (I+HS). 30 min after injury, normal saline and hydrogen-rich saline were injected intraperitoneally (5 mL/kg) in I+NS group and I+HS group, respectively. All rats were euthanized and blood and organ specimens were collected for determination 24 h after inhalation injury.
  
  
  RESULTS
  Tumor necrosis factor-alpha (TNF-α) levels, malondialdehyde (MDA) concentrations, nuclear factor kappa B (NF-κB) p65 expression, and apoptosis index (AI) in I+HS group were significantly decreased (P < 0.05), while superoxide dismutase (SOD) activities were increased compared with those in I+NS group; and a marked improvement in alveolar structure was also found after hydrogen-rich saline treatment.
  
  
  CONCLUSIONS
  Hydrogen-rich saline treatment exerts protective effects in acute lung injury induced by inhalation injury, at least in part through the activation of anti-inflammatory and antioxidant pathways and inhibition of apoptosis.
authors.affiliation: The Fourth Hospital of Tianjin, Tianjin 300222, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Tianjin Medical University, Tianjin 300070, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Tianjin Medical University, Tianjin 300070, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Fourth Hospital of Tianjin, Tianjin 300222, China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Feng
authors.affiliation: The Fourth Hospital of Tianjin, Tianjin 300222, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The Fourth Hospital of Tianjin, Tianjin 300222, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: The Fourth Hospital of Tianjin, Tianjin 300222, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D006859:Hydrogen / Q000494:pharmacology / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D021381:Protein Transport / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D015208:Smoke Inhalation Injury / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D013482:Superoxide Dismutase