title: Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.
abstract: Quorum sensing is a cell-to-cell communication mechanism, which is responsible for regulating a number of bacterial virulence factors and biofilm maturation and therefore plays an important role for establishing wound infection. Quorum-sensing signals may induce inflammation and predispose wounds to infection by Pseudomonas aeruginosa; however, the interaction has not been well investigated. We examined the effects of the P. aeruginosa las quorum-sensing signal, N-3-oxo-dodecanoyl homoserine lactone (3OC12-HSL), on matrix metalloproteinase (MMP) 9 expression in Rat-1 fibroblasts. 3OC12-HSL upregulated the expression of the MMP9 gene bearing an activator protein-1 (AP-1) binding site in the promoter region. We further investigated the mechanism underlying this effect. c-Fos gene expression increased rapidly after exposure to 3OC12-HSL, and nuclear translocation of c-Fos protein was observed; both effects were reduced by pretreatment with an AP-1 inhibitor. These results suggest that 3OC12-HSL can alter MMP9 gene expression in fibroblasts via the AP-1 signaling pathway.
authors.affiliation: a Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine , The University of Tokyo , Tokyo , Japan.
authors.forename: Gojiro
authors.initials: G
authors.lastname: Nakagami
authors.forename: Takeo
authors.initials: T
authors.lastname: Minematsu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Morohoshi
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamane
authors.forename: Toshiki
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Lijuan
authors.initials: L
authors.lastname: Huang
authors.forename: Mayumi
authors.initials: M
authors.lastname: Asada
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagase
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ikeda
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ikeda
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sanada
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D045784:Abietanes / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006714:Homoserine / Q000031:analogs & derivatives* / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism; D053038:Quorum Sensing; D051381:Rats; D015398:Signal Transduction; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D045784:Abietanes; C109860:N-(3-oxododecanoyl)homoserine lactone; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1; C021751:tanshinone; D006714:Homoserine; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat; D015107:4-Butyrolactone
keywords: 3-Oxododecanoyl homoserine lactone; inflammation; quorum sensing; wound infection