title: Fractone aging in the subventricular zone of the lateral ventricle.
abstract: In adulthood, the subventricular zone (SVZ) is one of the restricted places where neurogenesis persists. In this neurogenic niche, specialized extracellular matrix (ECM) structures termed fractones contact neural stem cells and their immediate progeny. Fractones are composed of ubiquitous ECM components including heparan sulfate proteoglycans such as perlecan and agrin. We have previously shown that fractones can capture growth factors and promote growth factor activity through a heparin binding mechanism in order to regulate neurogenesis. With aging, neurogenesis is known to decrease. However, the effect of aging on fractones structure and composition remains unknown. Here, we report that, while fractone number decreased, fractone size dramatically increased with aging. Despite the changes in fractones morphology, niche cells expressing glial fibrillary acidic protein kept direct contact with fractones. Furthermore, we have observed that heparan sulfate chains contained in fractones were modified with aging. However, FGF-2 was still captured by fractones via heparan sulfates. Together, our results suggest that the changes observed in fractones structure and composition are critically related to aging of the SVZ neurogenic niche.
authors.affiliation: Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Kerever
authors.affiliation: Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Taihei
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Tropical Medicine and Infectious Diseases, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
authors.forename: Frederic
authors.initials: F
authors.lastname: Mercier
authors.affiliation: Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, Japan; Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan. Electronic address: ehirasaw@juntendo.ac.jp.
authors.forename: Eri
authors.initials: E
authors.lastname: Arikawa-Hirasawa
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D005109:Extracellular Matrix / Q000737:chemistry / Q000473:pathology*; D006497:Heparan Sulfate / Q000378:metabolism; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D018613:Microscopy, Confocal; D058953:Neural Stem Cells / Q000166:cytology; D055495:Neurogenesis / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D055153:Stem Cell Niche / Q000502:physiology
chemical_list: D006497:Heparan Sulfate
keywords: Aging; Extracellular matrix; Fractone; Heparan sulfate proteoglycan; Subventricular zone