title: Acrylamide induces accelerated endothelial aging in a human cell model.
abstract: Acrylamide (AAM) has been recently discovered in food as a Maillard reaction product. AAM and glycidamide (GA), its metabolite, have been described as probably carcinogenic to humans. It is widely established that senescence and carcinogenicity are closely related. In vitro, endothelial aging is characterized by replicative senescence in which primary cells in culture lose their ability to divide. Our objective was to assess the effects of AAM and GA on human endothelial cell senescence. Human umbilical vein endothelial cells (HUVECs) cultured in vitro were used as model. HUVECs were cultured over 3 months with AAM or GA (1, 10 or 100 μM) until growth arrest. To analyze senescence, β-galactosidase activity and telomere length of HUVECs were measured by cytometry and semi-quantitative PCR, respectively. At all tested concentrations, AAM or GA reduced cell population doubling compared to the control condition (p < 0.001). β-galactosidase activity in endothelial cells was increased when exposed to AAM (≥10 μM) or GA (≥1 μM) (p < 0.05). AAM (≥10 μM) or GA (100 μM) accelerated telomere shortening in HUVECs (p < 0.05). In conclusion, in vitro chronic exposure to AAM or GA at low concentrations induces accelerated senescence. This result suggests that an exposure to AAM might contribute to endothelial aging.
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Sellier
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Boulanger
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France.
authors.forename: François
authors.initials: F
authors.lastname: Maladry
authors.affiliation: Institut Polytechnique de Lasalle Beauvais, EGEAL Unit, France.
authors.forename: Frédéric J
authors.initials: FJ
authors.lastname: Tessier
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Lorenzi
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Nevière
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Desreumaux
authors.affiliation: Department of Biostatistics, EA2694, CERIM, Lille School of Medicine, Lille University, Lille, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Beuscart
authors.affiliation: Geriatric Department, University Hospital of Lille, France.
authors.forename: François
authors.initials: F
authors.lastname: Puisieux
authors.affiliation: Inserm U995/Team "Glycation: From Inflammation to Aging", Lille School of Medicine, Lille University, France. Electronic address: nicolas.grossin@univ-lille2.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Grossin
mesh_terms: D020106:Acrylamide / Q000378:metabolism / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D015416:Maillard Reaction; D009994:Osmolar Concentration; D059506:Telomere Shortening / Q000187:drug effects; D059550:Toxicity Tests, Subchronic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D004852:Epoxy Compounds; D020106:Acrylamide; C071834:glycidamide; C061901:GLB1 protein, human; D001616:beta-Galactosidase
keywords: Acrylamide; Endothelial cell; Hayflick's limit; Senescence; Telomere length shortening