title: Alteration of mRNA and microRNA expression profiles in rat muscular type vasculature in early postnatal development.
abstract: The vascular system is characterized by a high degree of plasticity. In particular, functional and structural remodeling of the arterial system takes place during early postnatal development. However, the mechanisms providing such alterations in the rapidly growing organisms are poorly understood, especially for the peripheral vasculature. To explore this, we performed mRNA- and miRNA microarray analysis on muscular type saphenous arteries of young (10-12 days) and adult (2-3 months) rats. Thirty-eight significant pathways (such as oxidative phosphorylation, MAPK signaling, metabolism, cell cycle, DNA replication and focal adhesion) were obtained on differentially regulated genes during postnatal development. Many differentially regulated genes were determined as target- and miRNA-hubs. We also found 92 miRNAs differentially expressed in arteries of young and adult rats. Several significantly regulated pathways were found on these regulated miRNAs. Interestingly, these biological cascades also contain those significantly enriched pathways that were previously identified based on the differently expressed genes. Our data indicate that the expression of many genes involved in the regulation of pathways that are relevant for different functions in arteries may be under the control of miRNAs and these miRNAs regulate the functional, and structural remodeling occurring in the vascular system during early postnatal development.
authors.affiliation: 1] Cardiovascular Physiology, Centre for Biomedicine and Medical Technology Mannheim, Ruprecht-Karls-University Heidelberg, 68167 Mannheim, Germany [2] Faculty of Biology, M.V. Lomonosov Moscow State University, 119234, Moscow, Russia [3] Department of Physiology, Russian National Research Medical University, Ostrovityanova str. 1, 117997 Moscow, Russia.
authors.forename: Dina
authors.initials: D
authors.lastname: Gaynullina
authors.affiliation: Medical Research Center, University of Heidelberg, D-68167 Mannheim, Germany.
authors.forename: Harsh
authors.initials: H
authors.lastname: Dweep
authors.affiliation: Cardiovascular Physiology, Centre for Biomedicine and Medical Technology Mannheim, Ruprecht-Karls-University Heidelberg, 68167 Mannheim, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Gloe
authors.affiliation: Faculty of Biology, M.V. Lomonosov Moscow State University, 119234, Moscow, Russia.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Tarasova
authors.affiliation: Medical Research Center, University of Heidelberg, D-68167 Mannheim, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Sticht
authors.affiliation: Medical Research Center, University of Heidelberg, D-68167 Mannheim, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Gretz
authors.affiliation: Cardiovascular Physiology, Centre for Biomedicine and Medical Technology Mannheim, Ruprecht-Karls-University Heidelberg, 68167 Mannheim, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Schubert
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D019295:Computational Biology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D055806:Microvessels / Q000254:growth & development / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D018482:Muscle, Skeletal / Q000098:blood supply / Q000254:growth & development / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger