title: Pentoxifylline Accelerates Wound Healing Process by Modulating Gene Expression of MMP-1, MMP-3, and TIMP-1 in Normoglycemic Rats.
abstract: BACKGROUND
  Although numerous recent advances in wound healing have highlighted the importance of adjunct therapies in maximizing healing potential, abnormal wound healing continues to cause significant expense, morbidity, and mortality.
  
  
  OBJECTIVE
  This study examines the effects of pentoxifylline (PTX) on wound healing.
  
  
  OBJECTIVE
  We are expecting that PTX administration can increase biomechanical parameters and modulate matrix metalloproteinaseses-1 (MMP-1) and MMP-3, and matrix metalloproteinase inhibitor-1 (TIMP-1) in normoglycemic (NG) rat model of wound healing.
  
  
  METHODS
  Each rat received an identical wound from a full-thickness incision on its back. Experimental rats received systemic administration of PTX. All rats underwent examinations for biomechanical and polymerase chain reaction(PCR) analysis. Maximum stress and energy absorption were calculated as the biomechanical examinations. Expressions of MMP-1, MMP-3 and TIMP-1 genes were evaluated semi-quantitatively by reverse transcriptional (RT)-PCR.
  
  
  RESULTS
  PTX significantly improved biomechanical parameters. Quantification of MMP-1, MMP-3, and TIMP-1 showed that PTX significantly reduced gene expressions of MMP-1 and MMP-3. There was a significant increase in TIMP-1 gene expression.
  
  
  CONCLUSIONS
  Systemic administration of PTX significantly accelerated the wound healing process in NG rats.
authors.affiliation: 1Department of Anatomy, Medical Faculty, Arak University of Medical Sciences, Arak, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Babaei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001786:Blood Glucose / Q000032:analysis; D004353:Drug Evaluation, Preclinical; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis* / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis* / Q000235:genetics; D010431:Pentoxifylline / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001786:Blood Glucose; C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D019278:Matrix Metalloproteinase 3; C576840:MMP1 protein, rat; D020781:Matrix Metalloproteinase 1; D010431:Pentoxifylline
keywords: biomechanical evaluation; normoglycemic rats; pentoxifylline; reverse transcriptional polymerase chain reaction; wound healing