title: The activin-βA/BMP-2 chimera AB204 is a strong stimulator of adipogenesis.
abstract: Several of the bone morphogenetic proteins (BMPs) have been reported to induce white as well as brown adipogenesis. Here, we characterized the adipogenic potential of AB204, a recombinant chimeric protein of activin-βA and BMP-2, in in vitro, ex vivo and in vivo settings. BMP-2 is generally known to promote adipogenesis. When compared with BMP-2, which previously showed varying degrees of adipogenesis, AB204 displayed superior in vitro adipogenic differentiation of mouse 3 T3-L1 pre-adipocytes and human adipose-derived stem cells (hASCs). Surprisingly, implantation of hASCs, preconditioned with AB204 for as short a time as 48 h, into the subcutaneous space of athymic nude mice effectively produced fat pads, but not with BMP-2. When BMP-2 and AB204 were injected intraperitoneally, AB204 promoted dramatic systemic adipogenesis of C57BL/6 mice on a high-fat diet very effectively. The results implicate the novel clinical potential of AB204, including induction of fat tissue ex vivo or in vivo for tissue re-engineering and regenerative medicinal purposes, more than any known natural protein ligand. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Joint Centre for Biosciences, Lee Gil Ya Cancer and Diabetes Research Institute, Gachon University of Medicine and Science, Incheon, Korea.
authors.forename: Meejung
authors.initials: M
authors.lastname: Kim
authors.affiliation: National Creative Research Initiatives Centre for Adipose Tissue Remodelling, Department of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Republic of Korea.
authors.forename: Jong In
authors.initials: JI
authors.lastname: Kim
authors.affiliation: National Creative Research Initiatives Centre for Adipose Tissue Remodelling, Department of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Republic of Korea.
authors.forename: Jae Bum
authors.initials: JB
authors.lastname: Kim
authors.affiliation: Joint Centre for Biosciences, Lee Gil Ya Cancer and Diabetes Research Institute, Gachon University of Medicine and Science, Incheon, Korea.|Qualcomm Institute, University of California at San Diego, La Jolla, CA, USA.
authors.forename: Senyon
authors.initials: S
authors.lastname: Choe
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology; D006801:Humans; D028322:Inhibin-beta Subunits / Q000494:pharmacology*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C000597022:AB204; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004041:Dietary Fats; D011993:Recombinant Fusion Proteins; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits
keywords: BMP; TGFβ; adipogenesis; adipose-derived stem cells