title: Validation of algal viability treated with total residual oxidant and organic matter by flow cytometry.
abstract: Algal cell growth after starch and oxidant treatments in seawater species (Isochrysis galbana and Phaeodactylum tricornutum) and freshwater species (Selenastrum capricornutum and Scenedesmus obliquus) were evaluated by flow cytometry with fluorescein diacetate (FDA) staining to determine algal viability. Growth of algal cell was found to be significantly different among groups treated with NaOCl, starch and/or sodium thiosulfate, which are active substance (Total Residual Oxidant; TRO as Cl2), organic compound to meet efficacy testing standard and neutralizer of TRO by Ballast Water Management Convention of International Maritime Organization, respectively. The viability of algal cell treated with TRO in starch-add culture of 5days after treatment and neutralization was decreased significantly. ATP contents of the treated algal cells corresponded to the FL1 fluorescent signal of flow cytometry with FDA staining. I. galbana was the most sensitive to TRO-neutralized cultures during viability analysis.
authors.affiliation: Department of Biology, Kyung Hee University, Seoul, Republic of Korea. Electronic address: junghyun8809@naver.com.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biology, Kyung Hee University, Seoul, Republic of Korea. Electronic address: ejejej@naver.com.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Department of Biology, Kyung Hee University, Seoul, Republic of Korea. Electronic address: alga0720@naver.com.
authors.forename: Kitae
authors.initials: K
authors.lastname: Rhie
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000460:Chlorophyta / Q000166:cytology / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins / Q000378:metabolism; D005618:Fresh Water; D058087:Haptophyta / Q000166:cytology / Q000187:drug effects*; D016877:Oxidants / Q000494:pharmacology*; D044444:Scenedesmus / Q000166:cytology / Q000187:drug effects; D012623:Seawater; D012767:Ships; D013045:Species Specificity; D013213:Starch / Q000737:chemistry / Q000494:pharmacology; D018675:Toxicity Tests / Q000379:methods
chemical_list: D005452:Fluoresceins; D016877:Oxidants; D000255:Adenosine Triphosphate; D013213:Starch; C018506:diacetylfluorescein
keywords: ATP contents; Algal viability; Ballast water management; Flow cytometry; Organic matter; Total residual oxidant