title: Biophysicochemical Interaction of a Clinical Pulmonary Surfactant with Nanoalumina.
abstract: We report on the interaction of pulmonary surfactant composed of phospholipids and proteins with nanometric alumina (Al2O3) in the context of lung exposure and nanotoxicity. We study the bulk properties of phospholipid/nanoparticle dispersions and determine the nature of their interactions. The clinical surfactant Curosurf, both native and extruded, and a protein-free surfactant are investigated. The phase behavior of mixed surfactant/particle dispersions was determined by optical and electron microscopy, light scattering, and zeta potential measurements. It exhibits broad similarities with that of strongly interacting nanosystems such as polymers, proteins or particles, and supports the hypothesis of electrostatic complexation. At a critical stoichiometry, micron-sized aggregates arising from the association between oppositely charged vesicles and nanoparticles are formed. Contrary to the models of lipoprotein corona or of particle wrapping, our work shows that vesicles maintain their structural integrity and trap the particles at their surfaces. The agglomeration of particles in surfactant phase is a phenomenon of importance that could change the interactions of the particles with lung cells.
authors.affiliation: †Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS Université Denis Diderot Paris-VII, Bâtiment Condorcet, 10 rue Alice Domon et Léonie Duquet, 75205 Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Mousseau
authors.affiliation: ‡ImagoSeine Electron Microscopy Facility, Institut Jacques Monod, UMR 7592, CNRS - Université Paris Diderot Paris-VII, 75013 Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Le Borgne
authors.affiliation: †Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS Université Denis Diderot Paris-VII, Bâtiment Condorcet, 10 rue Alice Domon et Léonie Duquet, 75205 Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Seyrek
authors.affiliation: †Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS Université Denis Diderot Paris-VII, Bâtiment Condorcet, 10 rue Alice Domon et Léonie Duquet, 75205 Paris, France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Berret
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D055598:Chemical Phenomena; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D011663:Pulmonary Surfactants / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties; D013997:Time Factors
chemical_list: D010743:Phospholipids; D011663:Pulmonary Surfactants; D000537:Aluminum Oxide