title: Evolutionary Trends of the Transposase-Encoding Open Reading Frames A and B (orfA and orfB) of the Mycobacterial IS6110 Insertion Sequence.
abstract: BACKGROUND
  The IS6110 insertion sequence, a member of the IS3 family of insertion sequences, was found to be specific to the Mycobacterium tuberculosis complex (MTBC). Although IS6110 has been extensively characterized as a transposable genetic marker, the evolutionary history of its own transposase-encoding sequence has not, to the best of our knowledge, been investigated.
  
  
  RESULTS
  Here we explored the evolution of the IS6110 sequence by analysing the genetic variability and the selective forces acting on its transposase-encoding open reading frames (ORFs) A and B (orfA and orfB). For this purpose, we used a strain collection consisting of smooth tubercle bacilli (STB), an early branching lineage of the MTBC, and present-day M. tuberculosis strains representing the full breadth of genetic diversity in Tunisia. In each ORF, we found a major haplotype that dominated over a flat distribution of rare descendent haplotypes, consisting mainly of single- and double-nucleotide variant singletons. The predominant haplotypes consisted of both ancestral and present-day strains, suggesting that IS6110 acquisition predated the emergence of the MTBC. There was no evidence of recombination and both ORFs were subjected to strict purifying selection, as demonstrated by their dN/dS ratios (0.29 and 0.51, respectively), as well as their significantly negative Tajima's D statistics. Strikingly, the purifying selection acting on orfA proved much more stringent, suggesting its critical role in regulating the transpositional process. Maximum likelihood analyses further excluded any possibility of positive selection acting on single amino acid residues.
  
  
  CONCLUSIONS
  Taken together our data fit with an evolutionary scenario according to which the observed variability pattern of the IS6110 transposase-encoding ORFs is generated mainly through random point mutations that accrued on a functionally optimal IS6110 copy, whose acquisition predated the emergence of the MTBC complex. Background selection acting against deleterious mutations led to an excess of low-frequency variants.
authors.affiliation: Unit of Typing and Genetics of Mycobacteria, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development; Institut Pasteur de Tunis, Tunis, Tunisia.
authors.forename: Sara
authors.initials: S
authors.lastname: Thabet
authors.affiliation: Unit of Typing and Genetics of Mycobacteria, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development; Institut Pasteur de Tunis, Tunis, Tunisia.
authors.forename: Amine
authors.initials: A
authors.lastname: Namouchi
authors.affiliation: Unit of Typing and Genetics of Mycobacteria, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development; Institut Pasteur de Tunis, Tunis, Tunisia.
authors.forename: Helmi
authors.initials: H
authors.lastname: Mardassi
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D017354:Point Mutation; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D019895:Transposases / Q000235:genetics*; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases