title: O-2'-hydroxypropyltrimethyl ammonium chloride chitosan nanoparticles for the delivery of live Newcastle disease vaccine.
abstract: A novel complex chitosan derivative, O-2'-hydroxypropyltrimethyl ammonium chloride chitosan (O-2'-HACC), was synthesized and used to make nanoparticles as a delivery vehicle for live attenuated Newcastle disease vaccine. We found that O-2'-HACC had high antimicrobial activity, low toxicity, and a high safety level. Newcastle disease virus (NDV) was then encapsulated in the O-2'-HACC nanoparticles (NDV/La Sota-O-2'-HACC-NPs) by the ionic crosslinking method, and the properties of the resulting nanoparticles were determined by transmission electron microscopy, Zeta potential analysis, Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and X-ray diffraction. NDV/La Sota-O-2'-HACC-NPs had regular spherical morphologies and high stability, with an encapsulation efficiency of 95.68 ± 2.2% and a loading capacity of 58.75 ± 4.03%. An in vitro release assay indicated that release of NDV from NDV/La Sota-O-2'-HACC-NPs occurred slowly. Specific pathogen-free chickens immunized with NDV/La Sota-O-2'-HACC-NPs intranasally had much stronger cellular, humoral and mucosal immune responses than did those immunized intramuscularly or with live attenuated Newcastle disease vaccine. NDV/La Sota-O-2'-HACC-NPs are a novel drug delivery carrier with immense potential in medical applications.
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, China; Key Laboratory of Chemical Engineering Process & Technology for High-Efficiency Conversion, Heilongjiang University, Harbin 150080, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Dai
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Kang
authors.affiliation: Harbin Pharmaceutical Group Bio-Vaccine Co., Ltd., Harbin 150069, China.
authors.forename: Wanqiu
authors.initials: W
authors.lastname: Yang
authors.affiliation: Animal Husbandry Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Sun
authors.affiliation: Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081, China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Engineering Process & Technology for High-Efficiency Conversion, Heilongjiang University, Harbin 150080, China.
authors.forename: Guogang
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Rong
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Engineering Process & Technology for High-Efficiency Conversion, Heilongjiang University, Harbin 150080, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, China. Electronic address: zybin395@126.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004337:Drug Carriers; D016503:Drug Delivery Systems; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D066244:Proton Magnetic Resonance Spectroscopy; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014765:Viral Vaccines / Q000627:therapeutic use*; D014961:X-Ray Diffraction
chemical_list: D016207:Cytokines; D004337:Drug Carriers; D000644:Quaternary Ammonium Compounds; D014765:Viral Vaccines; C576941:hydroxypropyltrimethyl ammonium chloride chitosan; D048271:Chitosan
keywords: Chitosan derivatives; Mucosal immune delivery system; Nanoparticles; Newcastle disease; Sustained release