title: Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles.
abstract: Recent proof-of-principle studies demonstrated the suitability of the surface plasmon resonance imaging (SPRi) technique for the detection of individual submicrometer and nanoparticles in solutions. In the current study, we used the SPRi technique for visualization of the binding of round-shaped viruses (inactivated influenza A virus) and virus-like particles (human immunodeficiency virus (HIV)-based virus-like particles) to the functionalized sensor surface. We show the applicability of the SPRi technique for the detection of individual virus-like particles in buffers without serum as well as in buffers containing different concentrations of serum. Furthermore, we prove the specificity of visualized binding events using two different pseudotypes of HIV virus-like particles. We also demonstrate the applicability of the SPRi technique for the determination of relative particle concentrations in solutions. Moreover, we suggest a technical approach, which allows enhancing the magnitude of binding signals. Our studies indicate that the SPRi technique represents an efficient research tool for quantification and characterization of biological submicrometer objects such as viruses or virus-like particles, for example.
authors.affiliation: ISAS, Leibniz Institute for Analytical Sciences, Dortmund, Germany. Electronic address: viktoria.shpacovitch@isas.de.
authors.forename: Victoria
authors.initials: V
authors.lastname: Shpacovitch
authors.affiliation: Institute of Clinical and Molecular Virology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Temchura
authors.affiliation: Institute of Virology, Philipps University, Marburg, Germany.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Matrosovich
authors.affiliation: Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Hamacher
authors.affiliation: ISAS, Leibniz Institute for Analytical Sciences, Dortmund, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Skolnik
authors.affiliation: Department of Computer Science XII, TU Dortmund University, Dortmund, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Libuschewski
authors.affiliation: Department of Computer Science VII, TU Dortmund University, Dortmund, Germany.
authors.forename: Dominic
authors.initials: D
authors.lastname: Siedhoff
authors.affiliation: Department of Computer Science VII, TU Dortmund University, Dortmund, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Weichert
authors.affiliation: Department of Computer Science XII, TU Dortmund University, Dortmund, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Marwedel
authors.affiliation: Department of Computer Science VII, TU Dortmund University, Dortmund, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Müller
authors.affiliation: Institute of Clinical and Molecular Virology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Überla
authors.affiliation: ISAS, Leibniz Institute for Analytical Sciences, Dortmund, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Hergenröder
authors.affiliation: ISAS, Leibniz Institute for Analytical Sciences, Dortmund, Germany. Electronic address: alexander.zybin@isas.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Zybin
mesh_terms: D057809:HEK293 Cells; D006678:HIV / Q000737:chemistry; D006801:Humans; D009980:Influenza A virus / Q000378:metabolism*; D010316:Particle Size; D020349:Surface Plasmon Resonance / Q000379:methods*; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water
keywords: Biological nanoparticles; Biosensors; Detection of single nanoparticles; Surface plasmon resonance; Viruses