title: Analysis of the Humoral Immune Response to Human Leukocyte Antigens in Turkish Renal Transplant Candidates and Relationship Between Autoimmune Disorders.
abstract: BACKGROUND
  Pre-transplant sensitization to human leukocyte antigens (HLA) is a risk factor for graft failure. The purpose of the current single-center study was to evaluate humoral immune response to HLA antigens and the possible relationship between anti-HLA antibody titer and autoimmune disorders in renal transplant candidates.
  
  
  METHODS
  A total of 435 renal transplant candidates were analyzed; 50 sensitized patients were enrolled in this study. The HLA typing was performed by use of enzyme-linked immunoassay combined with Luminex technology. The patients were questioned for clinical evidence of hypothyroidism and systemic lupus erythematosus (SLE) and investigated for anti-nuclear antibody (ANA), anti-double-stranded DNA (anti-dsDNA), anti-thyroglobulin (anti-TG), anti-thyroid peroxidase (anti-TPO) antibodies, and thyroid function tests.
  
  
  RESULTS
  Among 50 patients with positive panel reactive antibody, 24 (48%) were positive for class I and negative for class II, 12 (24%) were negative for class I and positive for class II, and 14 (28%) were positive for both classes I and II. The specificities of anti-HLA antibodies-A23, A68, A69, B27, B49, DR6, and DR8-were the most frequent. ANA and anti-dsDNA antibodies were not correlated with either clinical symptoms of SLE or anti-HLA antibody titer of renal transplant candidates. Similarly, anti-TG and anti-TPO antibodies were not correlated with clinical hypothyroidism or anti-HLA antibody titer.
  
  
  CONCLUSIONS
  Our data have demonstrated the profile of anti-HLA antibodies in patients who were on the renal transplant waiting list in Turkey. The most frequent specificities of anti-HLA antibodies were A23, A68, A69, B27, B49, DR6, and DR8. There was no association between anti-HLA antibody titer and clinical and laboratory evidence of SLE and hypothyroidism.
authors.affiliation: Department of Nephrology, Kartal Research and Training Hospital, Istanbul, Turkey. Electronic address: elifaribakir@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Ari
authors.affiliation: Department of Transplantation, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Yildirim
authors.affiliation: Department of Transplantation, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: H F
authors.initials: HF
authors.lastname: Kucuk
authors.affiliation: Department of Transplantation, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Durmaz
authors.affiliation: Department of Transplantation, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Dogu
authors.affiliation: Department of Nephrology, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Yavuz
authors.affiliation: Department of Nephrology, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Parmaksiz
authors.affiliation: Department of Nephrology, Kartal Research and Training Hospital, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Asicioglu
authors.affiliation: Department of Immunology, Bilim University, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Carin
mesh_terms: D000328:Adult; D000974:Antibodies, Antinuclear / Q000276:immunology; D001323:Autoantibodies; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007037:Hypothyroidism / Q000276:immunology*; D056724:Immunity, Humoral / Q000502:physiology*; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000601:surgery; D016030:Kidney Transplantation; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014421:Turkey
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C006606:anti-thyroglobulin