title: Direct immobilization of antibodies on a new polymer film for fabricating an electrochemical impedance immunosensor.
abstract: A new polymer bearing aldehyde groups was designed and synthesized by grafting 4-pyridinecarboxaldehyde onto poly(epichlorohydrin). Antibodies can be directly immobilized on the surface of the polymer film through the covalent bonding of aldehyde groups of the film with amino groups of antibodies. In this study, human immunoglobulin G (IgG) was used as a model analyte for the fabrication of an electrochemical impedance immunosensor. Using the proposed immunosensor, IgG in the range from 0.1 to 80 ng ml(-1) was detected with a detection limit of 0.07 ng ml(-1) (signal/noise [S/N]=3). In addition, the electrochemical impedance immunosensor displays good stability and reproducibility.
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China. Electronic address: zhangxiangy06@163.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China. Electronic address: shenyouming79@tom.com.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yin
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry*; D008567:Membranes, Artificial
chemical_list: D055767:Immobilized Proteins; D007074:Immunoglobulin G; D008567:Membranes, Artificial
keywords: Convenient immobilization method; Electrochemical impedance immunosensor; Human immunoglobulin G; New matrix