title: Anticancer properties of low molecular weight oat beta-glucan – An in vitro study.
abstract: Anticancer properties of 1-3, 1-4 oat beta glucan are under intensive investigation now. Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation. Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application. According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1. The low molecular weight beta-glucan from oat significantly deceased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly deceased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic. Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative. Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland. Electronic address: anna.choromanska@umed.wroc.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Choromanska
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Julita
authors.initials: J
authors.lastname: Kulbacka
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Rembialkowska
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Pilat
authors.affiliation: Department of Food Biotechnology, Wroclaw University of Economics, Komandorska 118-120, 53-345 Wroclaw, Poland.
authors.forename: Remigiusz
authors.initials: R
authors.lastname: Oledzki
authors.affiliation: Department of Food Biotechnology, Wroclaw University of Economics, Komandorska 118-120, 53-345 Wroclaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Harasym
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Saczko
mesh_terms: D000328:Adult; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D018554:Avena / Q000737:chemistry*; D053454:Caspase 12 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D008297:Male; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008970:Molecular Weight; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000556:secondary; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D047071:beta-Glucans; C506444:CASP12 protein, human; D053454:Caspase 12
keywords: Melanoma; Oat beta-glucan; Skin cancer