title: Urine exoglycosidases are potential markers of renal tubular injury in children with ureteropelvic junction obstruction.
abstract: OBJECTIVE
  Hydronephrosis caused by ureteropelvic junction obstruction (UPJO) is an important problem in children and young adults. The aim of this pilot study was to determine the urine profiles of a number of lysosomal exoglycosidases to see whether they indicated tubular renal damage in children with UPJO.
  
  
  METHODS
  We measured lysosomal exoglycosidases urine activities in 32 patients with UPJO, dividing them into three groups. The surgical group comprised 16 children with severe hydronephrosis who required surgery, the nonsurgical group comprised 16 patients with mild hydronephrosis, and the reference group comprised 42 healthy children. The following indicators were measured: N-acetyl-β-hexosaminidase and its A and B isoenzymes, α-fucosidase, β-galactosidase, α-mannosidase and β-glucuronidase.
  
  
  RESULTS
  The urine activities of all exoglycosidases were significantly higher in children with UPJO than children in the reference group (p < 0.01). A strong positive correlation was also found between most of the urine exoglycosidases and the urine albumin/creatinine ratio (p < 0.01).
  
  
  CONCLUSIONS
  Our findings demonstrated that children with UPJO showed increased renal activities of assessed exoglycosidases, which correlated positively with the urine albumin/creatinine ratio. A larger multicentre study is required to confirm the clinical applications of these observations.
authors.affiliation: Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Taranta-Janusz
authors.affiliation: Department of Imaging Diagnostics, Children Hospital, Medical University of Białystok, Białystok, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Zalewska-Szajda
authors.affiliation: Medical Institute, College of Computer Science and Business Administration of Lomza, Lomza, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Chojnowska
authors.affiliation: Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wasilewska
mesh_terms: D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006026:Glycoside Hydrolases / Q000652:urine*; D006801:Humans; D007674:Kidney Diseases / Q000209:etiology* / Q000652:urine*; D007677:Kidney Function Tests; D007682:Kidney Pelvis; D007684:Kidney Tubules; D008297:Male; D010865:Pilot Projects; D014517:Ureteral Obstruction / Q000150:complications* / Q000628:therapy / Q000652:urine*
chemical_list: D015415:Biomarkers; D006026:Glycoside Hydrolases
keywords: Exoglycosidases; Hydronephrosis; Microalbuminuria; Renal damage; Ureteropelvic junction obstruction