title: [Application of Nested PCR in the Diagnosis of Imported Plasmodium Ovale Infection].
abstract: OBJECTIVE
  To identity Plasmodium ovale infection by 18S rRNA gene nested PCR.
  
  
  METHODS
  Whole blood and filter paper blood samples of malaria patients in Shandong Province were collected during 2012-2013. The parasites were observed under a microscope with Giemsa staining. The genome DNA of blood samples were extracted as PCR templates. Genus- and species-specific primers were designed according to the Plasmodium 18S rRNA gene sequences. Plasmodium ovale-positive specimens were identified by nested PCR as well as verified by sequencing.
  
  
  RESULTS
  There were 7 imported cases of P. ovale infection in the province during 2012-2013. Nested PCR results showed that the P. ovale specific band (800 bp) was amplified in all the 7 specimens. Blast results indicated that the PCR products were consistent with the Plasmodium ovale reference sequence in GenBank.
  
  
  CONCLUSIONS
  Seven imported cases of ovale malaria in Shandong Province in 2012-2013 are confirmed by nested PCR.
authors.forename: Bing-cheng
authors.initials: BC
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.forename: Kun
authors.initials: K
authors.lastname: Yin
authors.forename: Gong-zhen
authors.initials: GZ
authors.lastname: Liu
authors.forename: Wei-yan
authors.initials: WY
authors.lastname: Wang
authors.forename: Gui-hua
authors.initials: GH
authors.lastname: Zhao
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Wei
authors.forename: Yong-bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Chang-lei
authors.initials: CL
authors.lastname: Zhao
authors.forename: Qing-kuan
authors.initials: QK
authors.lastname: Wei
mesh_terms: D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics; D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D041122:Plasmodium ovale; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S