title: Mining the key regulatory genes of chicken inosine 5'-monophosphate metabolism based on time series microarray data.
abstract: IMP (inosine 5'-monophosphate) is a compound that enhances the flavor of poultry meat. IMP has become a new breeding trait to improve poultry meat quality. We tried to identify several potential regulatory genes, and construct their predicted regulatory relationships. Time series gene expression profiles of thigh muscle tissues of Rugao chicken, a famous indigenous breed in China, were performed for analysis of genes that are co-expressed or correlated with the concentration of IMP. We found 15 crucial co-expression genes, which are Hspa2, Pten, Gabpa, Bpi, Mkl1, Srf, Cd34, Hspa4, Etv6, Bmpr2, Gde1, Igfbp5, Cd28, Pecam1 and Gja1, that may directly or indirectly regulate IMP metabolism. Eventually, we computed the correlation coefficient between 19 IMP Genes and 15 CGs (15 co-expression genes), and we identified and constructed a predicted regulation network. In conclusion, variation of IMP concentration was primarily connected with the muscle development process. During this process, 15 CGs were identified that may have significant influence on IMP metabolism. In particular, Bmpr2, Pten and co-expression genes correlated with Entpd8 might play important roles in regulating IMP de novo synthesis, decomposition and salvage synthesis.
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Wang
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Chang
authors.affiliation: Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Ave., Yangzhou, Jiangsu 225009 P. R. China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Chen
keywords: Co-expression; Hub genes; IMP metabolism; Regulation network