title: Efficacy of anti-adhesion gel of carboxymethylcellulose with polyethylene oxide on peripheral nerve: Experimental results on a mouse model.
abstract: BACKGROUND
  Perineural scar formation is responsible for pain and loss of function after surgical procedures. Neurolysis and application of anti-adhesion gels are required to restore a gliding surface. We tested a carboxymethylcellulose (CMC) and polyethylene oxide (PEO) gel on mouse sciatic nerve to describe its safety and efficacy.
  
  
  METHODS
  Adult mice underwent a surgical procedure in which we burned the muscular bed of the sciatic nerve bilaterally (Burned group) and applied anti-adhesion gel to 1 of the nerves (Burned+gel group). After 3 weeks, we studied scar tissue by biomechanical and histological evaluation.
  
  
  RESULTS
  Both histological and biomechanical analysis showed that the gel reduced perineural scarring. The difference between the Burned and Burned+gel groups was statistically significant.
  
  
  CONCLUSIONS
  CMC-PEO gel can reduce perineural scar tissue. In histological section, scar tissue was present in both groups, but in the Burned+gel group a gliding surface was identified between scar and nerve.
authors.affiliation: Reconstructive Microsurgery Unit, Traumatology Department, CTO Hospital, Via Zuretti 29, 10100 Turin, Italy.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Tos
authors.affiliation: Reconstructive Microsurgery Unit, Traumatology Department, CTO Hospital, Via Zuretti 29, 10100 Turin, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Crosio
authors.affiliation: Plastic Surgery Unit, Department of Biotechnologies and Sciences of Life, University of Insubria/Varese, Varese, Italy.
authors.forename: Igor
authors.initials: I
authors.lastname: Pellegatta
authors.affiliation: Plastic Surgery Unit, Department of Biotechnologies and Sciences of Life, University of Insubria/Varese, Varese, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Valdatta
authors.affiliation: Human Anatomy Laboratory, Clinical and Biological Sciences Department, University of Turin, AOU San Luigi Gonzaga, Orbassano, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Pascal
authors.affiliation: Human Anatomy Laboratory, Clinical and Biological Sciences Department, University of Turin, AOU San Luigi Gonzaga, Orbassano, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Geuna
authors.affiliation: Plastic Surgery Unit, Department of Biotechnologies and Sciences of Life, University of Insubria/Varese, Varese, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Cherubino
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D002056:Burns / Q000503:physiopathology; D002266:Carboxymethylcellulose Sodium / Q000627:therapeutic use*; D004195:Disease Models, Animal; D051379:Mice; D008813:Mice, Inbred ICR; D010523:Peripheral Nervous System Diseases / Q000188:drug therapy* / Q000209:etiology; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D013501:Surface-Active Agents / Q000008:administration & dosage*
chemical_list: D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D002266:Carboxymethylcellulose Sodium
keywords: CMC-PEO; anti-adhesion gel; nerve repair; perineural adherence; peripheral nerve