title: [Effects of mesenchymal stem cells on angiogenesis of cervical cancer HeLa cancer cell line HeLa in vivo].
abstract: OBJECTIVE
  To explore the effects of bone marrow-derived mesenchymal stem cell (MSC) on angiogenesis of cervical cancer cell.
  
  
  METHODS
  MSCs from umbilical cord were mixed with cervical cancer cell line HeLa cells and subcutaneously plated in armpit area of nude mice to examine the in vivo tumor growth. Micro-vessel density (MVD) in tumor tissue was examined by CD34 immunohistochemical staining. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR) were employed to detect the expression of proangiogenesis factors.
  
  
  RESULTS
  MSC could effectively promote the cervical cancer growth in vivo as compared with controls (P<0.05). The immunohistochemical results of CD34 staining showed that MVD of cervical cancer was higher in MSC group than that in control group (P<0.05). And there was a higher expression of vascular endothelial growth factor (VEGF) in MSC than controls.
  
  
  CONCLUSIONS
  MSC promotes the in vivo angiogenesis of cervical cancer cell line by VEGF production.
authors.affiliation: Zhejiang University Hospital, Hangzhou 310058, China; Email: linlinbaiyitianshi@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
authors.forename: Lishuang
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006367:HeLa Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms; D042461:Vascular Endothelial Growth Factor A
chemical_list: D042461:Vascular Endothelial Growth Factor A