title: Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats.
abstract: Mesenchymal stem cells (MSCs) are an attractive source for the clinical cell therapy of liver injury. Although the use of adult serum, platelet lysate, or cord blood serum solves some of the problems caused by fetal bovine serum (FBS), the allogeneic immune response, contamination, and donor-to-donor and donor-to-receptor differences still obstruct the application of MSCs. In this study, the influences of autoserum from liver-injured rats (LIRs) and allogeneic serum from healthy rats on the isolation and culture of bone marrow MSCs (BMSCs) were examined and compared to FBS. The results showed that BMSCs cultured with autoserum or allogeneic serum exhibited better MSC-specific morphology, lower rate of cell senescent, and higher proliferation kinetics than those with FBS. In addition, autoserum promoted the osteogenic differentiation potential of BMSCs as allogeneic serum did. Although there were no significant differences in proliferation activity, immunophenotypic characterization, and differentiation potential between BMSCs cultured with autoserum and those with allogeneic serum, the potential adverse immunological reactions in patients with allogeneic material transplantation must be considered. We therefore believe that the autoserum from liver-injured patients may be a better choice for MSC expansion to meet the needs of liver injury therapy.
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Endocrinology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Meng