title: Reliable Detection and Smart Deletion of Malassez Counting Chamber Grid in Microscopic White Light Images for Microbiological Applications.
abstract: In biology, hemocytometers such as Malassez slides are widely used and are effective tools for counting cells manually. In a previous work, a robust algorithm was developed for grid extraction in Malassez slide images. This algorithm was evaluated on a set of 135 images and grids were accurately detected in most cases, but there remained failures for the most difficult images. In this work, we present an optimization of this algorithm that allows for 100% grid detection and a 25% improvement in grid positioning accuracy. These improvements make the algorithm fully reliable for grid detection. This optimization also allows complete erasing of the grid without altering the cells, which eases their segmentation.
authors.affiliation: 1Service Systèmes d'Information,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Denimal
authors.affiliation: 1Service Systèmes d'Information,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Ambroise
authors.initials: A
authors.lastname: Marin
authors.affiliation: 3UMR A 02.102 Procédés Alimentaires et Microbiologiques,équipe Procédés Microbiologiques et Biotechnologiques,AgroSup Dijon/Université de Bourgogne,1 esplanade Erasme,21000 Dijon,France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Guyot
authors.affiliation: 2Groupe Modélisation Numérique en Agronomie et AgroAlimentaire,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Journaux
authors.affiliation: 2Groupe Modélisation Numérique en Agronomie et AgroAlimentaire,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Paul
authors.initials: P
authors.lastname: Molin
mesh_terms: D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008828:Microbiological Techniques / Q000379:methods*; D008853:Microscopy / Q000379:methods*
keywords: Fourier transform; Malassez; homomorphic filtering; image analysis; noise filtering