title: Selection and evaluation of reference genes for expression analysis of Cassi.
abstract: Cassia obtusifolia, belonging to legume family, is important in many fields with high pharmaceutical, economic, and ecological values. These interests of C. obtusifolia triggered in-depth and fundamental genetic and molecular research. Therefore, the stable reference gene is necessary for normalization of the gene expression studies. In this study, 10 candidate reference genes were subjected to expression analysis in 12 different tissues and under different stresses by qRT-PCR. The expression stability was evaluated using geNorm, NormFinder, and BestKeeper software. In conclusion, different suitable reference genes were selected in different tissues and under different stress. CYP1, EF1α2, ACT2, UBQ1 were the most stable reference genes in all samples. The relative expression levels of WRKY gene were detected to confirm the reliability of the selected reference genes. These results provided suitable reference genes that could be used for normalization in C. obtusifolia tissues and under different stress.
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Zubi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: b College of Life Sciences , Zhejiang University , Hangzhou , China.
authors.forename: Qiankun
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Li
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Yu
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Xinhe
authors.initials: X
authors.lastname: Huang
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Wang
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Rui
authors.initials: R
authors.lastname: Tan
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: a School of Life Science and Engineering , Southwest Jiaotong University , Chengdu , China.
authors.forename: Hai
authors.initials: H
authors.lastname: Liao
mesh_terms: D002366:Cassia / Q000235:genetics*; D019295:Computational Biology; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012984:Software; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D010940:Plant Proteins
keywords: Cassia obtusifolia; quantitative real-time PCR; reference gene