title: Dynamic subcellular localization of a respiratory complex controls bacterial respiration.
abstract: Respiration, an essential process for most organisms, has to optimally respond to changes in the metabolic demand or the environmental conditions. The branched character of their respiratory chains allows bacteria to do so by providing a great metabolic and regulatory flexibility. Here, we show that the native localization of the nitrate reductase, a major respiratory complex under anaerobiosis in Escherichia coli, is submitted to tight spatiotemporal regulation in response to metabolic conditions via a mechanism using the transmembrane proton gradient as a cue for polar localization. These dynamics are critical for controlling the activity of nitrate reductase, as the formation of polar assemblies potentiates the electron flux through the complex. Thus, dynamic subcellular localization emerges as a critical factor in the control of respiration in bacteria.
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: François
authors.initials: F
authors.lastname: Alberge
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: Leon
authors.initials: L
authors.lastname: Espinosa
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: Farida
authors.initials: F
authors.lastname: Seduk
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: Léa
authors.initials: L
authors.lastname: Sylvi
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: René
authors.initials: R
authors.lastname: Toci
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Walburger
authors.affiliation: Laboratoire de Chimie Bactérienne UMR7283, Institut de Microbiologie de la Méditerranée, Centre national de la recherche scientifique, Aix Marseille Université, Marseille, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Magalon
mesh_terms: D002458:Cell Fractionation; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009097:Multienzyme Complexes / Q000378:metabolism*; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D021122:Protein Subunits / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D021122:Protein Subunits; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C508365:NarG protein, E coli; D050901:Nitrate Reductase
keywords: E. coli; cell biology; infectious disease; microbiology; nitrate reductase; protonmotive force; respiration