title: Detecting total toxicity in water using a mediated biosensor system with flow injection.
abstract: A novel total toxicity detection method based on a mediated biosensor system with flow injection (MB-FI) was developed to rapidly and reliably detect respiration inhibitors (i.e., As2O3, KCN, salicylic acid (SA), 2,4-dintirophenol (DNP)) in water. The mediated biosensor toxicity assessment using microorganisms immobilized in calcium alginate filaments can greatly simplify the testing process and save time. In the MB-FI system, ferricyanide together with a respiration inhibitor was injected into the bioreactor, inhibiting the respiration of the immobilized microorganisms. The degree of inhibition was measured by determining the ferrocyanide generated in the effluent, expressed as the 50% inhibition concentration (IC50). The IC50 values for the four respiration inhibitors obtained using this method were comparable to those obtained using the classic method, confirming that this approach is an alternative alert method. More importantly, this constructed biosensor system with flow injection will facilitate the application and commercialization of this toxicity monitoring technology.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Daming
authors.initials: D
authors.lastname: Yong
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Chengzhou
authors.initials: C
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Dengbin
authors.initials: D
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhai
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: dongsj@ciac.jl.cn.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
mesh_terms: D019297:2,4-Dinitrophenol / Q000032:analysis / Q000633:toxicity; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005295:Ferrocyanides / Q000737:chemistry; D017022:Flow Injection Analysis; D010087:Oxides / Q000032:analysis / Q000633:toxicity; D011190:Potassium Cyanide / Q000032:analysis / Q000633:toxicity; D016958:Pseudomonas putida / Q000187:drug effects* / Q000254:growth & development; D020156:Salicylic Acid / Q000032:analysis / Q000633:toxicity; D014867:Water; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D001152:Arsenicals; D005295:Ferrocyanides; D010087:Oxides; D014874:Water Pollutants, Chemical; D014867:Water; D011190:Potassium Cyanide; D020156:Salicylic Acid; D019297:2,4-Dinitrophenol; D000077237:Arsenic Trioxide
keywords: Flow injection mode; Immobilized microorganism; Mediated biosensor; Total toxicity