title: Single Molecules, Cells, and Super-Resolution Optics (Nobel Lecture).
abstract: The resolution of a microscope is determined by the diffraction limit in classical microscopy, whereby objects that are separated by half a wavelength can no longer be visually separated. To go below the diffraction limit required several tricks and discoveries. In his Nobel Lecture, E. Betzig describes the developments that have led to modern super high-resolution microscopy.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Dr., Ashburn, VA 20147 (USA).
authors.forename: Eric
authors.initials: E
authors.lastname: Betzig
mesh_terms: D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*
keywords: PALM; microscopy; near-field; super-resolution