title: Subcellular Targeting of Proteins Involved in Modification of Plant N- and O-Glycosylation.
abstract: Plants are attractive expression hosts for the production of recombinant glycoprotein therapeutics. The quality and efficiency of these biopharmaceuticals are very often influenced by the glycosylation profile. Consequently, approaches are needed that enable the production of recombinant glycoproteins with customized and homogenous N- and O-glycan structures. Here, we describe convenient tools that allow targeting and retention of glycan-modifying enzymes in the early secretory pathway of plants. These protocols can be used to fine-tune the subcellular localization of glycosyltransferases and glycosidases in plants and consequently to increase the homogeneity of glycosylation on recombinant glycoproteins.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Dicker
authors.forename: Jennifer
authors.initials: J
authors.lastname: Schoberer
authors.forename: Ulrike
authors.initials: U
authors.lastname: Vavra
authors.forename: Richard
authors.initials: R
authors.lastname: Strasser
mesh_terms: D006023:Glycoproteins / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides; D011994:Recombinant Proteins; D016695:Glycosyltransferases; D006026:Glycoside Hydrolases