title: Cloning and characterization of spliced variants of the porcine G protein coupled receptor 120.
abstract: The polyunsaturated fatty acids (PUFAs) receptor GPR120 exerts a significant impact on systemic nutrient homeostasis in human and rodents. However, the porcine GPR120 (pGPR120) has not been well characterized. In the current study, we found that pGPR120 had 3 spliced variants. Transcript 1 encoded 362-amino acids (aa) wild type pGPR120-WT, which shared 88% homology with human short form GPR120. Transcript 1 was the mainly expressed transcript of pGPR120. It was expressed predominantly in ileum, jejunum, duodenum, spleen, and adipose. Transcript 3 (coding 320-aa isoform) was detected in spleen, while the transcript 2 (coding 310-aa isoform) was only slightly expressed in spleen. A selective agonist for human GPR120 (TUG-891) and PUFAs activated SRE-luc and NFAT-luc reporter in HEK293T cells transfected with construct for pGPR120-WT but not pGPR120-V2. However, 320-aa isoform was not a dominant negative isoform. The extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation levels in cells transfected with construct for pGPR120-WT were well activated by PUFAs, especially n-3 PUFA. These results showed that although pGPR120 had 3 transcripts, transcript 1 which encoded pGPR120-WT was the mainly expressed transcript. TUG-891 and PUFAs, especially n-3 PUFA, well activated pGPR120-WT. The current study contributed to dissecting the molecular regulation mechanisms of n-3 PUFA in pigs.
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Tongxing
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Hong-Kui
authors.initials: HK
authors.lastname: Wei
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
mesh_terms: D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics; D013552:Swine
chemical_list: D005231:Fatty Acids, Unsaturated; D043562:Receptors, G-Protein-Coupled