title: A study of diagnostic criteria established for two oral mucous diseases by HMME-fluorescence spectroscopy.
abstract: Malignant oral ulcers are common pathological occurrence in oral and maxillofacial tumors. A noninvasive method for diagnosis of malignant oral ulcers was developed in the study, which is based on hematoporphyrin monomethylether (HMME) fluorescence spectroscopy. The objective of this work is to determine the feasibility of this method in differentiating the malignant tissues from the inflammatory ones in the hamster cheek pouch model. Adult hamsters were used for the study and a cheek pouch model was established. For the malignant model, the 9, 10-dimethyl-1, 2-benzanthracene carcinogenesis was applied to one cheek pouch for 10 weeks (N = 35). The simple ulcers were created on buccal cheek mucosa in a simple manner (N = 10). Prior to sacrifice, HMME solution was injected into the tissues. The induced fluorescence spectra of the cheek tissues were recorded by a fiber spectrometer with excitation at 405 nm. A spectral algorithm was used to eliminate the effect of autofluorescence, and a spectral parameter S was selected as diagnostic criterion. After fluorescence measurement, the animals were sacrificed and the measured tissues were collected. Histological staining was performed and the results of histopathological evaluation were documented. The diagnostic criteria that reflected the fluorescence intensity were set as follows: normal, S ≤ 10; simple ulcer, 230 ≤ S ≤ 290; and malignant ulcer, 140 ≤ S ≤ 200. The sensitivity and specificity of this detection method was verified by scalpel biopsy, and the overall accuracy was over 90%. The results of this study showed that the fluorescence spectroscopic method implemented by HMME can accurately differentiate the two kinds of clinically indistinguishable diseases.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People's Republic of China.
authors.forename: Moyang
authors.initials: M
authors.lastname: Lv
authors.affiliation: Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin, 150080, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People's Republic of China. hao621126@126.com.
authors.forename: Limin
authors.initials: L
authors.lastname: Mao
authors.affiliation: Department of Preventive Dentistry, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People's Republic of China. jenipherzl@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People's Republic of China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Lv
authors.affiliation: BoRui Dental Clinic, Harbin, 150001, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.affiliation: Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin, 150080, People's Republic of China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006224:Cricetinae; D006415:Hematoporphyrins / Q000737:chemistry*; D008027:Light; D008647:Mesocricetus; D009059:Mouth Diseases / Q000175:diagnosis*; D009061:Mouth Mucosa / Q000473:pathology*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D006415:Hematoporphyrins; C494379:hematoporphyrin monomethyl ether
keywords: HMME; Oral erosions; Oral ulcer; Photodynamic diagnosis