title: Reproductive toxicity of chlorpyrifos tested in zebrafish (Danio rerio): Histological and hormonal end points.
abstract: This study was carried out to find out whether exposure to environmentally relevant concentration of chlorpyrifos (CP) modulates reproductive competence. To understand this, eight adult male and eight adult female zebrafish (Danio rerio) were exposed to 200 µg/L of CP for 24, 48, 72, and 96 h. Vitellogenin levels did not show much change in female fish, whereas in male the levels increased with increasing exposure time thereby indicating estrogenecity of the toxicant. Attenuation of serum 11-ketotestosterone in male and serum 17β-estradiol in female was noticed in the exposed fish and thus signified interference of CP in the reproductive endocrine system. Structural damage common to both the gonads was vacuolization. Elongation of seminiferous tubules in testes and atretic follicles in ovary was also observed.
authors.affiliation: Department of Zoology, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India Department of Life Sciences, Universidad de las Fuerzas Armadas-ESPE, Quito, Ecuador, South America.
authors.forename: Bangeppagari
authors.initials: B
authors.lastname: Manjunatha
authors.affiliation: Department of Zoology, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India ghphilip@rediffmail.com.
authors.forename: Gundala Harold
authors.initials: GH
authors.lastname: Philip
mesh_terms: D000818:Animals; D004390:Chlorpyrifos / Q000633:toxicity*; D004958:Estradiol / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006066:Gonads / Q000187:drug effects* / Q000473:pathology; D008297:Male; D013739:Testosterone / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D014819:Vitellogenins / Q000097:blood / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004958:Estradiol; D004390:Chlorpyrifos; C003600:11-ketotestosterone
keywords: Chlorpyrifos; gonadal histology; reproductive hormones; zebrafish (Danio rerio)