title: Assessment of plasma brain-derived neurotrophic factor (BDNF), activity-dependent neurotrophin protein (ADNP) and vasoactive intestinal peptide (VIP) concentrations in treatment-naïve humans with multiple sclerosis.
abstract: OBJECTIVE
  Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS) characterized by coexisting processes of inflammation, demyelination, axonal neurodegeneration and gliosis. Autoimmune processes play a pivotal role in the disease. The immune system may be modulated by neurotrophins and neurotrophin factors. Aim of the study was to assess plasma levels of brain-derived neurotrophic factor (BDNF), activity-dependent neurotrophin protein (ADNP) and vasoactive intestinal peptide (VIP) in treatment-naïve humans with newly diagnosed multiple sclerosis. We also elucidated the potential influence of selected inflammatory agents on peripheral concentration of BDNF and ADNP.
  
  
  METHODS
  The study population comprised of 31 untreated patients with MS and 36 controls from a single hospital centre. Assessment of BDNF and ADNP was performed with use of ELISA methods. VIP was measured with RIA. Selected cytokine levels (IL 6, IL 10, and TNF α) were evaluated with ELISA tests. Statistical analyses were performed.
  
  
  RESULTS
  We failed to find any significant differences between ADNP, BDNF, VIP, CRP levels and concentration of cytokines between individuals with MS and the controls. No correlation was observed between ADNP, BDNF and VIP as the first parameter and CRP, IL 6, IL 10, TNFα levels and the Expanded Disability Status Scale score in MS.
  
  
  CONCLUSIONS
  Newly diagnosed, treatment-naïve patients with MS have comparable levels of plasma BDNF, ADNP and VIP to those of healthy controls.
authors.affiliation: Department of Neurology, Medical University of Warsaw, Bielanski Hospital, Warsaw,, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Kochanowski
authors.affiliation: Department of Neurology, Medical University of Warsaw, Bielanski Hospital, Warsaw,, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Uchman
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Litwiniuk
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kalisz
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wolinska-Witort
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Martynska
authors.affiliation: Department of Neurology, Medical University of Warsaw, Bielanski Hospital, Warsaw,, Poland.
authors.forename: Boguslawa
authors.initials: B
authors.lastname: Baranowska
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bik
mesh_terms: D000328:Adult; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood*; D005260:Female; D018398:Homeodomain Proteins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood*; D009419:Nerve Tissue Proteins / Q000097:blood*; D014660:Vasoactive Intestinal Peptide / Q000097:blood*; D055815:Young Adult
chemical_list: C492178:ADNP protein, human; D019208:Brain-Derived Neurotrophic Factor; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D014660:Vasoactive Intestinal Peptide; C579455:BDNF protein, human