title: Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples.
abstract: An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. In this work, a novel molecularly imprinted polymer composite membrane (MIM) was synthesized and employed for the selective detection in urine samples of 2-deoxyadenosine (2-dA), an important tumoral marker. By thermal polymerization, the 2-dA-MIM was cross-linked on the surface of a polyvinylidene-difluoride (PVDF) membrane. By characterization techniques, the linking of the imprinted polymer on the surface of the membrane was found. Batch-wise guest binding experiments confirmed the absorption capacity of the synthesized membrane towards the template molecule. Subsequently, a time-course of 2-dA retention on membrane was performed and the best minimum time (30 min) to bind the molecule was established. HPLC analysis was also performed to carry out a rapid detection of target molecule in urine sample with a recovery capacity of 85%. The experiments indicated that the MIM was highly selective and can be used for revealing the presence of 2-dA in urine samples.
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy. sonia.scorrano@unisalento.it.
authors.forename: Sonia
authors.initials: S
authors.lastname: Scorrano
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy. lucia.mergola@unisalento.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mergola
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy. mariapia.dibello@unisalento.it.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Di Bello
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy. mariarosaria.lazzoi@unisalento.it.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Lazzoi
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy. giuseppe.vasapollo@unisalento.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Vasapollo
authors.affiliation: Dipartimento di Ingegneria dell'Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy. roberta.delsole@unisalento.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Del Sole
mesh_terms: D065966:Absorption, Physicochemical; D003188:Composite Resins / Q000737:chemistry*; D003839:Deoxyadenosines / Q000652:urine*; D006801:Humans; D008567:Membranes, Artificial; D054802:Molecular Imprinting; D011145:Polyvinyls / Q000737:chemistry; D016482:Urinalysis / Q000379:methods*; D005465:Fluorocarbon Polymers
chemical_list: D003188:Composite Resins; D003839:Deoxyadenosines; D008567:Membranes, Artificial; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C058118:2'-deoxyadenosine
keywords: 2-deoxyadenosine; molecularly imprinted membrane (MIM); selective separation; urine detection