title: Preparation and characterization of silver nanoparticle loaded amorphous hydrogel of carboxymethylcellulose for infected wounds.
abstract: There is a growing demand for an appropriate and safe antimicrobial dressing to treat infected deep wounds. An amorphous gel formulation (SNP-CMC), containing silver nanoparticles (SNPs) and carboxymethylcellulose (CMC), was prepared in one step by the reduction of silver nitrate in situ. Spectrophotometric and microscopic analysis revealed that the SNPs were 7-21 nm in diameter. In simulated wound experiments, SNP-CMC gel was found to absorb 80.48 ± 4.69% w/w of saline and donate 17.43 ± 0.76% w/w of moisture within 24h indicating its dual fluid affinity. Cytocompatibility of the gel was assessed by proliferation studies with primary human skin cells. The antimicrobial activity studies showed that SNP-CMC containing 50 ppm of SNPs was effective against the growth of both Gram negative and Gram positive strains including methicillin-resistant Staphylococcus aureus (MRSA). These results indicate that SNP-CMC could be ideal for the treatment of deep infected wounds.
authors.affiliation: Department of Tissue Engineering & Wound Management, Regenerative Medicine, Reliance Life Sciences, Rabale, Navi Mumbai, Maharashtra 400701, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Das
authors.affiliation: Department of Tissue Engineering & Wound Management, Regenerative Medicine, Reliance Life Sciences, Rabale, Navi Mumbai, Maharashtra 400701, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Microbiology, Sri Harkishandas Narottamdas Hospital, Reliance Hospital Management Services, Girgaon, Mumbai, Maharashtra 400004, India.
authors.forename: Niranjan B
authors.initials: NB
authors.lastname: Patil
authors.affiliation: Department of Tissue Engineering & Wound Management, Regenerative Medicine, Reliance Life Sciences, Rabale, Navi Mumbai, Maharashtra 400701, India.
authors.forename: Chandra
authors.initials: C
authors.lastname: Viswanathan
authors.affiliation: Department of Tissue Engineering & Wound Management, Regenerative Medicine, Reliance Life Sciences, Rabale, Navi Mumbai, Maharashtra 400701, India. Electronic address: deepatghosh@hotmail.com.
authors.forename: Deepa
authors.initials: D
authors.lastname: Ghosh
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001458:Bandages; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D054368:Laxatives / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D012834:Silver / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy*
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D054368:Laxatives; D012834:Silver; D002266:Carboxymethylcellulose Sodium
keywords: Amorphous gel; Antimicrobial; Carboxymethylcellulose; Hydrogel wound dressing; Silver nanoparticles