title: Expression of circulating miR-486 and miR-150 in patients with acute myocardial infarction.
abstract: BACKGROUND
  With its high morbidity and mortality, acute myocardial infarction (AMI) places a major burden on society and on individual patients. Correct, early correct diagnosis is crucial to the management of AMI.
  
  
  METHODS
  In this study, the expression of circulating miR-486 and miR-150 was investigated in AMI patients and the two miRNAs were evaluated as potential biomarkers for AMI. Plasma samples from 110 patients with AMI (65 patients with ST-segment elevation myocardial infarction (STEMI) and 45 patients with non-ST-segment elevation myocardial infarction (NSTEMI)) and 110 healthy adults were collected. Circulating levels of miR-486 and miR-150 were detected using quantitative real-time PCR in plasma samples.
  
  
  RESULTS
  Results showed that the levels of miR-486 and miR-150 were significantly higher in AMI patients than in healthy controls. Receiver operating characteristic (ROC) curve analyses indicated that the two plasma miRNAs were of significant diagnostic value for AMI, especially NSTEMI. The combined ROC analysis revealed an AUC value of 0.771 in discriminating AMI patients from healthy controls and an AUC value of 0.845 in discriminating NSTEMI patients from healthy controls.
  
  
  CONCLUSIONS
  Results indicated that the levels of circulating miR-486 and miR-150 are associated with AMI. They may be novel and powerful biomarkers for AMI, especially for NSTEMI.
authors.affiliation: Department of emergency, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, Henan, 450052, China. ruizhang0001@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of emergency, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, Henan, 450052, China. lanchao29@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Lan
authors.affiliation: Department of emergency, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, Henan, 450052, China. 13526545517@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Pei
authors.affiliation: Department of emergency, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, Henan, 450052, China. dgyduck@163.com.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Duan
authors.affiliation: Department of emergency, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, Henan, 450052, China. 304817694@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of emergency, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, Henan, 450052, China. lilieme@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015331:Cohort Studies; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D011446:Prospective Studies; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C533097:MIR150, human; C560945:MIRN486 microRNA, human