title: Synthesis, crystal structure and biological activity of two Mn complexes with 4-acyl pyrazolone derivatives.
abstract: In order to study the biological activities of transitional metal complexes based on 4-acyl pyrazolone derivatives, two Mn complexes [Mn(HLa)(La)]·(CH3CN)1.5·H2O (1) and [Mn2(Lb)2(μ-EtO)2(EtOH)2] (2) (H2La = N-(1-phenyl-3-methyl-4-benzoyl-5-pyrazolone)-2-thiophenecarboxylic acid hydrazide, H2Lb = N-(1-phenyl-3-methyl-4-propenylidene-5-pyrazolone)-2-thiophenecarboxylic acid hydrazide) have been synthesized and characterized. Single crystal X-ray diffraction analysis indicated that 1 is a mononuclear complex and 2 exhibits a dinuclear centrosymmetric structure. Binding of the complexes with Herring Sperm DNA (HS-DNA) showed that complexes 1 and 2 could intercalate to DNA with quenching constant of 5.3×10(4) M(-1) and 4.9×10(4) M(-1), respectively. The interactions of the complexes with bovine serum albumin (BSA) indicated that complexes 1 and 2 could quench the intrinsic fluorescence of BSA in a static quenching process. Further, the inhibitory effects of the complexes on the cell population growth of the human esophageal cancer Eca-109 cells and the cervical cancer HeLa cells were determined by MTT assay, which indicated that both 1 and 2 significantly inhibited the growth of Eca-109 and HeLa cells, the inhibitory activity of complex 1 is stronger than that of 2. We further observed that complex 1 inhibited the growth of HeLa cells through inducing the apoptosis and arresting cell cycle at S phase. Our results suggested that both complexes 1 and 2 have DNA- and protein-binding capacity and antitumor activity.
authors.affiliation: Key Laboratory of Material and Technology for Clean Energy, Ministry of Education, PR China; Key Laboratory of Advanced Functional Materials, Autonomous Region, PR China; Institute of Applied Chemistry, Xinjiang University, Urumqi, 830046 Xinjiang, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830046 Xinjiang, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Material and Technology for Clean Energy, Ministry of Education, PR China; Key Laboratory of Advanced Functional Materials, Autonomous Region, PR China; Institute of Applied Chemistry, Xinjiang University, Urumqi, 830046 Xinjiang, PR China.
authors.forename: Chuan-Chuan
authors.initials: CC
authors.lastname: He
authors.affiliation: Key Laboratory of Material and Technology for Clean Energy, Ministry of Education, PR China; Key Laboratory of Advanced Functional Materials, Autonomous Region, PR China; Institute of Applied Chemistry, Xinjiang University, Urumqi, 830046 Xinjiang, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830046 Xinjiang, PR China.
authors.forename: Su-Rong
authors.initials: SR
authors.lastname: Sun
authors.affiliation: Key Laboratory of Material and Technology for Clean Energy, Ministry of Education, PR China; Key Laboratory of Advanced Functional Materials, Autonomous Region, PR China; Institute of Applied Chemistry, Xinjiang University, Urumqi, 830046 Xinjiang, PR China. Electronic address: xuguancheng@163.com.
authors.forename: Guan-Cheng
authors.initials: GC
authors.lastname: Xu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D008345:Manganese / Q000737:chemistry*; D011720:Pyrazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D011720:Pyrazoles; D012545:Schiff Bases; D013778:Tetrazolium Salts; D013844:Thiazoles; D012710:Serum Albumin, Bovine; D008345:Manganese; D004247:DNA; C022616:thiazolyl blue
keywords: 4-Acyl pyrazolone derivatives; Antitumor activity; Crystal structure; DNA-binding; Manganese complex; Protein binding