title: Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata.
abstract: We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS). Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81±1.84ngg(-1) wet tissue mass (WTM) and then started to decrease to a minimum of 1.57±0.74ngg(-1) WTM at the seventh week (p<0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p<0.001 and p<0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p<0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p<0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p<0.05). Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication.
authors.affiliation: IQUIBICEN-Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2° Pabellón, 4° piso, Ciudad Universitaria, (CP 1428) Buenos Aires, Argentina; Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2° Pabellón, 4° piso, Ciudad Universitaria, (1428) Buenos Aires, Argentina. Electronic address: sabatini@bg.fcen.uba.ar.
authors.forename: Sebastián E
authors.initials: SE
authors.lastname: Sabatini
authors.affiliation: Departamento de Biociencias, Cátedras de Bioquímica, Facultad de Química, Universidad de la República, Montevideo, Uruguay. Electronic address: bbrena@fq.edu.uy.
authors.forename: Beatríz M
authors.initials: BM
authors.lastname: Brena
authors.affiliation: Departamento de Biociencias, Cátedras de Bioquímica, Facultad de Química, Universidad de la República, Montevideo, Uruguay. Electronic address: macapirez@yahoo.com.
authors.forename: Macarena
authors.initials: M
authors.lastname: Pirez
authors.affiliation: IQUIBICEN-Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2° Pabellón, 4° piso, Ciudad Universitaria, (CP 1428) Buenos Aires, Argentina. Electronic address: mcrios@qb.fcen.uba.ar.
authors.forename: María Del Carmen Ríos
authors.initials: Mdel C
authors.lastname: de Molina
authors.affiliation: LEA, INIBIOMA-CONICET-Universidad Nacional del Comahue, CEAN, Junín de los Andes, Argentina. Electronic address: luquet@gmail.com.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Luquet
mesh_terms: D000818:Animals; D000975:Antioxidants; D003386:Brachyura / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000493:pharmacokinetics / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D043143:Hepatopancreas / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D052998:Microcystins / Q000493:pharmacokinetics / Q000633:toxicity*; D046931:Microcystis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D052998:Microcystins; D014874:Water Pollutants, Chemical; C078588:microcystin; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: ELISA; Estuarine crab; Hepatopancreas; Microcystin depuration; Toxin bioaccumulation