title: A universal entropy-driven mechanism for thioredoxin-target recognition.
abstract: Cysteine residues in cytosolic proteins are maintained in their reduced state, but can undergo oxidation owing to posttranslational modification during redox signaling or under conditions of oxidative stress. In large part, the reduction of oxidized protein cysteines is mediated by a small 12-kDa thiol oxidoreductase, thioredoxin (Trx). Trx provides reducing equivalents for central metabolic enzymes and is implicated in redox regulation of a wide number of target proteins, including transcription factors. Despite its importance in cellular redox homeostasis, the precise mechanism by which Trx recognizes target proteins, especially in the absence of any apparent signature binding sequence or motif, remains unknown. Knowledge of the forces associated with the molecular recognition that governs Trx-protein interactions is fundamental to our understanding of target specificity. To gain insight into Trx-target recognition, we have thermodynamically characterized the noncovalent interactions between Trx and target proteins before S-S reduction using isothermal titration calorimetry (ITC). Our findings indicate that Trx recognizes the oxidized form of its target proteins with exquisite selectivity, compared with their reduced counterparts. Furthermore, we show that recognition is dependent on the conformational restriction inherent to oxidized targets. Significantly, the thermodynamic signatures for multiple Trx targets reveal favorable entropic contributions as the major recognition force dictating these protein-protein interactions. Taken together, our data afford significant new insight into the molecular forces responsible for Trx-target recognition and should aid the design of new strategies for thiol oxidoreductase inhibition.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, Jupiter, FL 33458.
authors.forename: Prakash B
authors.identifier: http://orcid.org/0000-0001-8997-0860
authors.initials: PB
authors.lastname: Palde
authors.affiliation: Department of Chemistry, The Scripps Research Institute, Jupiter, FL 33458 kcarroll@scripps.edu.
authors.forename: Kate S
authors.initials: KS
authors.lastname: Carroll
mesh_terms: D001665:Binding Sites; D019277:Entropy; D004926:Escherichia coli / Q000201:enzymology; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D011487:Protein Conformation; D013879:Thioredoxins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D013879:Thioredoxins; D010088:Oxidoreductases; C052449:3'-phosphoadenylyl-5'-phosphosulfate reductase
keywords: entropy; oxidative stress; protein–protein interactions; redox regulation; thioredoxin