title: Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK).
abstract: This study was conducted to test the effect of DnaK on priming immune responses in Penaeid shrimp. Juvenile-specific pathogen-free (SPF) P. vannamei shrimp were injected with 0.05 μg recombinant DnaK. One hour post-DnaK priming, a non-lethal dose of Vibrio campbellii (10(5) CFU shrimp(-1)) was injected. Other treatments include only DnaK or V. campbellii injection or control with blank inocula. The haemolymph of three shrimp from each treatment was collected at 1.5, 6, 9 and 12 h post-DnaK priming (hpp). It was verified that injection with DnaK and V. campbellii challenge affected the transcription of 3 immune genes, transglutaminase-1 (TGase-1), prophenoloxidase-2 (proPO-2) and endogenous HSP70 (lvHSP70). In P. monodon, shrimp were first injected with DnaK at a dose of 10 μg shrimp(-1) and one hour later with 10(6) CFU of V. harveyi (BB120) shrimp(-1). Shrimp injected with DnaK showed a significant increase in proPO expression compared to the control (P < 0.05). Yet a double injection (DnaK and Vibrio) seemed to cause an antagonistic response at the level of expression, which was not equalled at the level of PO activity. Those results suggest that DnaK is able to modulate immune responses in P. vannamei and P. monodon.
authors.affiliation: Research Institute for Aquaculture No2, Ho Chi Minh City, Vietnam.
authors.forename: L H
authors.initials: LH
authors.lastname: Phuoc
authors.affiliation: Laboratory for Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Hu
authors.affiliation: Laboratory for Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Wille
authors.affiliation: Research Institute for Aquaculture No2, Ho Chi Minh City, Vietnam.
authors.forename: N T
authors.initials: NT
authors.lastname: Hien
authors.affiliation: Research Institute for Aquaculture No2, Ho Chi Minh City, Vietnam.
authors.forename: V H
authors.initials: VH
authors.lastname: Phuong
authors.affiliation: Research Institute for Aquaculture No2, Ho Chi Minh City, Vietnam.
authors.forename: N T N
authors.initials: NT
authors.lastname: Tinh
authors.affiliation: Research Institute for Aquaculture No2, Ho Chi Minh City, Vietnam.
authors.forename: N H
authors.initials: NH
authors.lastname: Loc
authors.affiliation: Laboratory for Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Sorgeloos
authors.affiliation: Laboratory for Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Bossier
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000494:pharmacology*; D007107:Immune System / Q000187:drug effects; D007155:Immunologic Factors / Q000494:pharmacology; D033561:Penaeidae / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D011503:Transglutaminases / Q000235:genetics; D014733:Vibrio / Q000276:immunology
chemical_list: D004792:Enzyme Precursors; D018840:HSP70 Heat-Shock Proteins; D007155:Immunologic Factors; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D011503:Transglutaminases; C105804:transglutaminase 1
keywords: DnaK heat-shock proteins; Penaeus monodon; Penaeus vannamei; Vibrio; heat-shock protein; recombinant protein