title: [Analysis of twin-arginine translocation system gene homology and transcription in Vibrio species].
abstract: OBJECTIVE
  To determine the function of twin-arginine translocation system (Tat) and gene cluster in Vibrio strains and to analyze the homology of tat gene cluster among different Vibrio spp. strains based on N16961 and tatABC mutant strains N169-dtat.
  
  
  METHODS
  Different serotypes of biotype strains of Vibrio spp. were selected to detect the transcription of 4 genes of Tat transport system and upstream ubi aarF gene and downstream cyt551 gene by the total RNA reverse transcription and homologicity of the gene cluster by sequencing analysis.
  
  
  RESULTS
  Our results showed that the 4 genes of tat cluster (tatA, tatB, tatC, and tatE) were intragenic and co-transcribed. We found that ubi aarF gene could be co-transcribed with tatA, tatB, but not with tatC. The electron transport chain and energy metabolism-related genes, cytochrome C551 peroxidase gene, and 4 genes located at upstream of tatABC operon were not transcribed with tatABC. Although the co-transcription between ubi aarF and tatAB was blocked in N169-dtat strain, they were still transcribed separately. Homologous analysis of genes of tat cluster in different types of Vibrio cholerae showed that tat gene cluster was a very conservative.
  
  
  CONCLUSIONS
  The ubi and aarF gene might be co-transcribed with genes of tat cluster in Vibrio cholerae, which and the close relationship showed that they might play a key function in Vibrio cholerae.
authors.affiliation: Pathogen Diagnosis and Biosafety Department, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
authors.forename: Zhaoqin
authors.initials: Z
authors.lastname: Zhu
authors.forename: Haili
authors.initials: H
authors.lastname: Chen
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhou
authors.forename: Huaiqi
authors.initials: H
authors.lastname: Jing
authors.forename: Meiying
authors.initials: M
authors.lastname: Yan
authors.forename: Shubei
authors.initials: S
authors.lastname: Zai
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Cai
authors.forename: Yunwen
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Email: kanbiao@icdc.cn.
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D001120:Arginine; D001426:Bacterial Proteins; D003574:Cytochrome c Group; D026901:Membrane Transport Proteins; D014734:Vibrio cholerae
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D026901:Membrane Transport Proteins; C017167:cytochrome C(551); D001120:Arginine