title: Simple synthesis of biocompatible biotinylated porous hexagonal ZnO nanodisc for targeted doxorubicin delivery against breast cancer cell: In vitro and in vivo cytotoxic potential.
abstract: Targeted drug delivery with porous materials features great promise as improved therapeutic potential for treatment of various diseases. In the present study we have attempted a microwave synthesis of porous hexagonal nanodisc of zinc oxide (PZHD) for the first time and its subsequent targeted delivery to breast cancer cells, MCF7. PZHD has been fabricated suitably with 3-aminopropyltriethoxysilane to impart additional stability and surface amines to anchor site directing ligand NHS-biotin. Biotinylated scaffold showed targeted delivery of anticancer drug doxorubicin and pH triggered release to MCF 7 cells with preferential distribution on specified domain. A detailed in vitro cytotoxicity study was associated with it to evaluate the mode of action of Dox loaded PZHD on MCF-7 cells by means of cell cycle analysis, apoptosis assays, Western blot and immuno-fluorescence image analysis. The efficacy of the Dox loaded PZHD was further validated from our in vivo tumor regression studies. Finally, the whole study has been supported by in vitro and in vivo bio-safety studies which also signified its biocompatibility with real time applications. To the best of our knowledge this is the first effort to use biotinylated PZHD for targeted delivery of doxorubicin within MCF 7 cells with a detailed study of its mechanistic application. This study might thus hold future prospects for therapeutic intervention for treatment of cancer.
authors.affiliation: Centre for Research in NanoScience and NanoTechnology, Technology Campus, University of Calcutta, JD 2, Sec III, Salt lake, Kolkata 700098, India. Electronic address: bioprasun@gmail.com.
authors.forename: Prasun
authors.initials: P
authors.lastname: Patra
authors.affiliation: Biological Sciences Division, Indian Statistical Institute, 203 B.T. Road, Kolkata 700108, India.
authors.forename: Shouvik
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Department of Biotechnology, Birla Institute of Technology and Science, Vidya Vihar, Pilani, Rajasthan 333031, India.
authors.forename: Amarto
authors.initials: A
authors.lastname: Das Gupta
authors.affiliation: Biological Sciences Division, Indian Statistical Institute, 203 B.T. Road, Kolkata 700108, India.
authors.forename: Saheli
authors.initials: S
authors.lastname: Pradhan
authors.affiliation: Centre for Research in NanoScience and NanoTechnology, Technology Campus, University of Calcutta, JD 2, Sec III, Salt lake, Kolkata 700098, India.
authors.forename: Saurav
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Centre for Research in NanoScience and NanoTechnology, Technology Campus, University of Calcutta, JD 2, Sec III, Salt lake, Kolkata 700098, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Ahir
authors.affiliation: Department of Physiology, University College of Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: Biological Sciences Division, Indian Statistical Institute, 203 B.T. Road, Kolkata 700108, India.
authors.forename: Sampad
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Biological Sciences Division, Indian Statistical Institute, 203 B.T. Road, Kolkata 700108, India.
authors.forename: Subhrodeb
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Physiology, University College of Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
authors.forename: Sreya
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: Centre for Research in NanoScience and NanoTechnology, Technology Campus, University of Calcutta, JD 2, Sec III, Salt lake, Kolkata 700098, India.
authors.forename: Arghya
authors.initials: A
authors.lastname: Adhikary
authors.affiliation: Biological Sciences Division, Indian Statistical Institute, 203 B.T. Road, Kolkata 700108, India.
authors.forename: Arunava
authors.initials: A
authors.lastname: Goswami
authors.affiliation: Centre for Research in NanoScience and NanoTechnology, Technology Campus, University of Calcutta, JD 2, Sec III, Salt lake, Kolkata 700098, India.
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Chattopadhyay
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001672:Biocompatible Materials; D001710:Biotin / Q000378:metabolism*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004317:Doxorubicin / Q000008:administration & dosage*; D006801:Humans; D066298:In Vitro Techniques; D061986:MCF-7 Cells; D051379:Mice; D049329:Nanostructures; D013056:Spectrophotometry, Ultraviolet; D015034:Zinc Oxide / Q000008:administration & dosage*
chemical_list: D000903:Antibiotics, Antineoplastic; D001672:Biocompatible Materials; D001710:Biotin; D004317:Doxorubicin; D015034:Zinc Oxide
keywords: Antitumor agents; Cytotoxicity; Drug delivery; Porous; Zinc oxide