title: Detection of trace metallic elements in oral lichenoid contact lesions using SR-XRF, PIXE, and XAFS.
abstract: Oral lichen planus (OLP) and oral lichenoid contact lesions (OLCL) are chronic inflammatory mucocutaneous reactions with a risk of malignant transformation that alter the epithelium. OLP and OLCL have similar clinical and histopathological features and it is difficult to distinguish one from the other. Metallic restorations are suspected to generate OLCLs. Trace metal analysis of OLCL specimens may facilitate the discrimination of symptoms and identification of causative metallic restorations. The purpose of this study was to assess OLCL tissue samples for the prevalence of metallic elements derived from dental restorations, and to discriminate OLCL from OLP by using synchrotron radiation-excited X-ray fluorescence analysis (SR-XRF), particle-induced X-ray emission (PIXE), and X-ray absorption fine structure (XAFS). Typical elements of dental materials were detected in the OLCL, whereas no obvious element accumulation was detected in OLP and negative control specimens. The origin of the detected metallic elements was presumed to be dental alloys through erosion. Therefore, our findings support the feasibility of providing supporting information to distinguish OLCL from OLP by using elemental analysis.
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sugiyama
authors.affiliation: 1] Advanced Biomaterials Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan [2] Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Uo
authors.affiliation: Advanced Biomaterials Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Wada
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Omagari
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komiyama
authors.affiliation: Graduate School of Science, Chiba University, Chiba, Japan.
authors.forename: Serika
authors.initials: S
authors.lastname: Miyazaki
authors.affiliation: Graduate School of Science, Chiba University, Chiba, Japan.
authors.forename: Chiya
authors.initials: C
authors.lastname: Numako
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Tadahide
authors.initials: T
authors.lastname: Noguchi
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Jinbu
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Kusama
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000328:Adult; D003722:Dental Alloys / Q000009:adverse effects; D005260:Female; D006801:Humans; D017676:Lichen Planus, Oral / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism* / Q000473:pathology; D014131:Trace Elements / Q000378:metabolism*
chemical_list: D003722:Dental Alloys; D014131:Trace Elements