title: Profiling leucocyte subsets in tuberculosis-diabetes co-morbidity.
abstract: The immune system plays an important role in the pathogenesis of pulmonary tuberculosis-type 2 diabetes mellitus (PTB-DM) co-morbidity. However, the phenotypic profile of leucocyte subsets at homeostasis in individuals with active or latent tuberculosis (LTB) with coincident diabetes is not known. To characterize the influence of diabetes on leucocyte phenotypes in PTB or LTB, we examined the frequency (Fo ) of leucocyte subsets in individuals with TB with (PTB-DM) or without (PTB) diabetes; individuals with latent TB with (LTB-DM) or without (LTB) diabetes and non-TB-infected individuals with (NTB-DM) or without (NTB) diabetes. Coincident DM is characterized by significantly lower Fo of effector memory CD4(+) T cells in LTB individuals. In contrast, DM is characterized by significantly lower Fo of effector memory CD8(+) T cells and significantly higher Fo of central memory CD8(+) T cells in PTB individuals. Coincident DM resulted in significantly higher Fo of classical memory B cells in PTB and significantly higher Fo of activated memory and atypical B cells in LTB individuals. Coincident DM resulted in significantly lower Fo of classical and intermediate monocytes in PTB, LTB and NTB individuals. Finally, DM resulted in significantly lower Fo of myeloid and plasmacytoid dendritic cells in PTB, LTB and NTB individuals. Our data reveal that coincident diabetes alters the cellular subset distribution of T cells, B cells, dendritic cells and monocytes in both individuals with active TB and those with latent TB, thus potentially impacting the pathogenesis of this co-morbid condition.
authors.affiliation: National Institutes of Health-NIRT-International Centre for Excellence in Research, Chennai, India.|National Institute for Research in Tuberculosis, Chennai, India.
authors.forename: Nathella Pavan
authors.initials: NP
authors.lastname: Kumar
authors.affiliation: National Institutes of Health-NIRT-International Centre for Excellence in Research, Chennai, India.
authors.forename: Kadar
authors.initials: K
authors.lastname: Moideen
authors.affiliation: National Institutes of Health-NIRT-International Centre for Excellence in Research, Chennai, India.
authors.forename: Sharmila D
authors.initials: SD
authors.lastname: Dhakshinraj
authors.affiliation: National Institute for Research in Tuberculosis, Chennai, India.
authors.forename: Vaithilingam V
authors.initials: VV
authors.lastname: Banurekha
authors.affiliation: National Institute for Research in Tuberculosis, Chennai, India.
authors.forename: Dina
authors.initials: D
authors.lastname: Nair
authors.affiliation: National Institute for Research in Tuberculosis, Chennai, India.
authors.forename: Chandrakumar
authors.initials: C
authors.lastname: Dolla
authors.affiliation: National Institute for Research in Tuberculosis, Chennai, India.
authors.forename: Paul
authors.initials: P
authors.lastname: Kumaran
authors.affiliation: National Institutes of Health-NIRT-International Centre for Excellence in Research, Chennai, India.
authors.forename: Subash
authors.initials: S
authors.lastname: Babu
mesh_terms: D000328:Adult; D000368:Aged; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology; D015415:Biomarkers / Q000032:analysis; D015897:Comorbidity; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007194:India / Q000453:epidemiology; D055985:Latent Tuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology* / Q000382:microbiology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology* / Q000382:microbiology; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: B cells; T cells; dendritic cells; diabetes; monocytes; tuberculosis