title: Cyanidin-3-O-β-glucoside Purified from Black Rice Protects Mice against Hepatic Fibrosis Induced by Carbon Tetrachloride via Inhibiting Hepatic Stellate Cell Activation.
abstract: This study investigated whether cyanidin-3-O-β-glucoside (Cy-3-G), a predominant anthocyanin, could exert a protective role on liver injury and its further mechanisms of the anti-fibrosis actions in mice. The results demonstrated that the treatment of Cy-3-G (800 mg/kg diet) for 8 weeks significantly attenuated hepatotoxicity and fibrosis in carbon tetrachloride (CCl4) administered mice. Cy-3-G strongly down-regulated the expression of α-smooth muscle actin (α-SMA), desmin, and matrix metalloproteinase (MMPs), which showed its suppression effect on the activation of hepatic stellate cells (HSCs). In addition, Cy-3-G favorably regulated oxidative stress and apoptosis in liver. Furthermore, Cy-3-G ameliorated the infiltration of inflammatory cells such as neutrophils and leukocytes and meanwhile suppressed the production of pro-inflammatory cytokines and growth factors. In conclusion, daily intake of Cy-3-G could prevent liver fibrosis progression in mice induced by CCl4 through inhibiting HSC activation, which provides a basis for clinical practice of liver fibrosis prevention.
authors.affiliation: †Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: §Department of Nutrition, Henry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, People's Republic of China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Guo
authors.affiliation: †Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Tianran
authors.initials: T
authors.lastname: Shen
authors.affiliation: †Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Xilan
authors.initials: X
authors.lastname: Tang
authors.affiliation: †Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: †Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.|‡Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, People's Republic of China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ling
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage* / Q000302:isolation & purification; D002251:Carbon Tetrachloride / Q000009:adverse effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005960:Glucosides / Q000008:administration & dosage* / Q000302:isolation & purification; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012275:Oryza / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification; D020011:Protective Agents / Q000008:administration & dosage* / Q000302:isolation & purification
chemical_list: D000872:Anthocyanins; D016207:Cytokines; D005960:Glucosides; D010936:Plant Extracts; D020011:Protective Agents; C462279:cyanidin-3-O-beta-glucopyranoside; D002251:Carbon Tetrachloride
keywords: anthocyanins; cyanidin-3-O-β-glucoside; hepatic stellate cells; liver fibrosis