title: Ultrasound enhanced enzymatic hydrolysis of Parthenium hysterophorus: A mechanistic investigation.
abstract: This study has attempted to establish the mechanism of the ultrasound-induced enhancement of enzymatic hydrolysis of pretreated and delignified biomass of Parthenium hysterophorus. A dual approach of statistical optimization of hydrolysis followed by application of sonication at optimum conditions has been adopted. The kinetics of hydrolysis shows a marked 6× increase with sonication, while net sugar yield shows marginal rise of ∼ 20%. The statistical experimental design reveals the hydrolysis process to be enzyme limited. Profile of sugar yield in ultrasound-assisted enzymatic hydrolysis has been analyzed using HCH-1 model coupled with Genetic Algorithm optimization. The trends in the kinetic and physiological parameters of HCH-1 model reveal that sonication enhances enzyme/substrate affinity and reaction velocity of hydrolysis. The product inhibition of enzyme in all forms (free, adsorbed, complexed) also reduces with ultrasound. These effects are attributed to intense micro-convection induced by ultrasound and cavitation in the liquid medium.
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Shuchi
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Mayank
authors.initials: M
authors.lastname: Agarwal
authors.affiliation: Department of Chemical Engineering, National Institute of Technology Tiruchirappalli, Tiruchirappalli 620 015, Tamil Nadu, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Bhatt
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India. Electronic address: vmoholkar@iitg.ernet.in.
authors.forename: Vijayanand S
authors.initials: VS
authors.lastname: Moholkar
mesh_terms: D019659:Asteraceae / Q000737:chemistry / Q000528:radiation effects; D044602:Cellulases / Q000737:chemistry* / Q000528:radiation effects; D003198:Computer Simulation; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000528:radiation effects; D006868:Hydrolysis; D008956:Models, Chemical; D010312:Parthenogenesis; D010936:Plant Extracts / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D013010:Sonication / Q000379:methods*; D000069453:Ultrasonic Waves; D000097762:Parthenium hysterophorus
chemical_list: C519376:Parthenium hysterophorus extract; D010936:Plant Extracts; D044602:Cellulases
keywords: Cavitation; Hydrolysis; P. hysterophorus; Reducing sugar; Sonication