title: Studies on antioxidative and immunostimulating fucogalactan of the edible mushroom Macrolepiota dolichaula.
abstract: A water soluble fucogalactan (PS-II) of an average molecular weight ∼1.2×10(5) Da was isolated from the aqueous extract of an edible mushroom Macrolepiota dolichaula. It was composed of fucose, galactose and 3-O-methyl galactose in a molar ratio of nearly 1:4:1. Structural characterization of PS-II was carried out using total hydrolysis, methylation analysis, Smith degradation, and 1D/2D NMR experiments. These results indicated that the proposed repeating unit of the PS-II had a backbone chain consisting of four (1→6)- linked α-d-Galp residues, one residue methylated at O-3, and another one substituted at O-2 by (1→2)-α-d-Galp residue, which is terminated with a α-l-Fucp moiety. The PS-II exhibited the antioxidant properties in different in vitro test systems, and also showed in vitro macrophage activation in RAW 264.7 cell line as well as splenocyte and thymocyte activation in mouse cell culture medium.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India.
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Pattanayak
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal 721302, India.
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
authors.forename: Somanjana
authors.initials: S
authors.lastname: Khatua
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal 721302, India.
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D000067996:RAW 264.7 Cells; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000975:Antioxidants; D005685:Galactans; C031159:fucogalactan
keywords: Antioxidant; Fucogalactan; Immunostimulation; Macrolepiota dolichaula; NMR spectroscopy