title: The Utilities of Chemical Reactions and Molecular Tools for O-GlcNAc Proteomic Studies.
abstract: The post-translational modification of nuclear and cytoplasmic proteins with O-linked β-N-acetylglucosamine (O-GlcNAc) is involved in a wide variety of cellular processes and is associated with the pathological progression of chronic diseases. Considering its emerging biological significance, systematic identification, site mapping, and quantification of O-GlcNAc proteins are essential and have led to the development of several approaches for O-GlcNAc protein profiling. This minireview mainly focuses on the various useful chemical reactions and molecular tools with detailed reaction mechanisms widely adopted for O-GlcNAc protein/peptide enrichment and its quantification for comprehensive O-GlcNAc protein profiling.
authors.affiliation: Department of Science Education-Chemistry Major, Daegu University, Gyeongsan-si, GyeongBuk 712-714 (Republic of Korea). eunkim@daegu.ac.kr, eunjukim151@gmail.com.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Kim
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis*; D000818:Animals; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
chemical_list: D010455:Peptides; D011506:Proteins; D000117:Acetylglucosamine
keywords: O-GlcNAc; enrichment technologies; molecular tools; proteomics; reaction mechanisms