title: Development of a New Paenibacillin-Producing Strain and Testing its Usability in Improving Food Safety.
abstract: A new bacterial strain that produces a bacteriocin (paenibacillin) without polymyxin was developed from Paenibacillus polymyxa that co-produces the 2 antimicrobial agents. Gamma radiation was used successfully to develop the new strain, P. polymyxa OSY-HG. Subsequently, we explored the feasibility of using food or food ingredients as growth media for the new strain. Milk supported the growth of P. polymyxa OSY-HG which produced up to 32 mg paenibacillin/L milk without polymyxin. Fermentation crude extract was applied in a model food (Vienna sausage) to control Listeria innocua, a Listeria monocytogenes surrogate. The treatment increased Listeria lag time by 2 d at 7 °C and at least 6 h at 37 °C. In conclusion, a new paenibacillin-producing P. polymyxa strain has been developed for potential industrial use. Using the new strain in applications that enhance food safety is feasible.
  
  
  CONCLUSIONS
  As low concentrations of paenibacillin can inhibit Listeria in a food matrix, paenibacillin has potential to be used as a natural food preservative to deter this pathogen’s growth in food, after FDA approval.
authors.affiliation: Dept. of Microbiology, The Ohio State Univ., 2015 Fyffe Court, Columbus, OH, U.S.A.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Gerst
authors.affiliation: Dept. of Food Science and Technology, The Ohio State Univ., 2015 Fyffe Court, Columbus, OH, U.S.A.
authors.forename: En
authors.initials: E
authors.lastname: Huang
authors.affiliation: Mass Spectrometry and Proteomics Facility, Campus Chemical Instrument Center, The Ohio State Univ., 460 W. 12th Ave. 209, Biomedical Research Tower, Columbus, OH, U.S.A.
authors.forename: Liwen
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Dept. of Microbiology, The Ohio State Univ., 2015 Fyffe Court, Columbus, OH, U.S.A.|Dept. of Food Science and Technology, The Ohio State Univ., 2015 Fyffe Court, Columbus, OH, U.S.A.
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Yousef
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis*; D001430:Bacteriocins / Q000096:biosynthesis; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D005240:Feasibility Studies; D005285:Fermentation; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D005520:Food Preservatives / Q000378:metabolism; D059022:Food Safety / Q000379:methods*; D005720:Gamma Rays; D008087:Listeria / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects; D008461:Meat Products / Q000382:microbiology; D008892:Milk; D056507:Paenibacillus / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D023181:Antimicrobial Cationic Peptides; D001430:Bacteriocins; D003470:Culture Media; D004269:DNA, Bacterial; D005520:Food Preservatives; C535070:paenibacillin N; C535069:paenibacillin P
keywords: Listeria; bacteriocin; gamma irradiation; meat product; paenibacillin