title: High Rab27A expression indicates favorable prognosis in CRC.
abstract: BACKGROUND
  Rab27A is a peculiar member in Rab family and has been suggested to play essential roles in the development of human cancers. However, the association between Rab27A expression and clinicopathological characteristics of colorectal cancer (CRC) has not been elucidated yet.
  
  
  METHODS
  One-step quantitative real-time polymerase chain reaction (qPCR) test with 18 fresh-frozen CRC samples and immunohistochemistry (IHC) analysis in 112 CRC cases were executed to evaluate the relationship between Rab27A expression and the clinicopathological features of CRC. Cox regression and Kaplan-Meier survival analyses were performed to identify the prognostic factors for 112 CRC patients.
  
  
  RESULTS
  The results specified that the expression levels of Rab27A mRNA and protein were significantly higher in CRC tissues than that in matched non-cancerous tissues, in both qPCR test (p = 0.029) and IHC analysis (p = 0.020). The IHC data indicated that the Rab27A protein expression in CRC was statistically correlated with lymph node metastasis (p = 0.022) and TNM stage (p = 0.026). Cox multi-factor analysis and Kaplan-Meier method suggested Rab27A protein expression (p = 0.012) and tumor differentiation (p = 0.004) were significantly associated with the overall survival of CRC patients.
  
  
  CONCLUSIONS
  The data indicated the differentiate expression of Rab27A in CRC tissues and matched non-cancerous tissues. Rab27A may be used as a valuable prognostic biomarker for CRC patients.
authors.affiliation: Department of Pathology and Pathophysiology, School of Medicine, Southeast University, Nanjing, 210009, China.
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Traumatic Surgery, Changzhou No. 2 People's Hospital Affiliated with Nanjing Medical University, Changzhou, 213000, China.
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Nanjing, 210000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Hou
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Nanjing, 210000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Hepatobiliary and Laparoscopic Surgery, YiXing People's Hospital, the Affiliated YiXing Hospital of Jiangsu University, Yixing, 214200, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhan
authors.affiliation: Department of Pathology and Laboratory Medicine, the Affiliated Hospital of Nantong University, Nantong, 226000, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China.|The Key Laboratory of Cancer Biomarkers, Prevention & Treatment Cancer Center and The Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Pathology and Pathophysiology, School of Medicine, Southeast University, Nanjing, 210009, China. chenps@seu.edu.cn.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Chen
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002454:Cell Differentiation; D016009:Chi-Square Distribution; D003082:Colectomy; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000032:analysis* / Q000235:genetics; D000075745:rab27 GTP-Binding Proteins
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D000075745:rab27 GTP-Binding Proteins; C066694:RAB27A protein, human; D020691:rab GTP-Binding Proteins