title: Upregulated TCRζ improves cytokine secretion in T cells from patients with AML.
abstract: Previous studies indicated that upregulating TCRζ partially recovers T cell function in patients with leukemia. In this study, we characterized the cytokine profile of TCRζ-transfected T cells from acute myeloid leukemia (AML) patients by QuantibodyArray Glass Chip. Firstly, the significantly lower expression of TCRζ in CD3(+)/TCRζ(+) cells from AML patients was found. Increased secretion of IL-2, IL-8, IL-10, IL-13, IFN-γ, TNF-α, GM-CSF, growth-regulated oncogene (GRO), MIP-1b, and regulated on activation, normal T cell expressed and secreted (RANTES) could be detected in T cells from AML patients after TCRζ upregulating. We concluded that upregulating TCRζ in T cells from AML can alter the secretion profile of cytokines and chemokine which are involved in T cell proliferation and activation.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. jnshaohuachen@163.com.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. 94603483@qq.com.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zha
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. 497823791@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. 251582936@qq.com.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. jnyanglijian@163.com.
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. jnlibo517@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. siulier@163.com.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. zhch2503@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. 931174453@qq.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. yhl2006jn@163.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. tzhuker@jnu.edu.cn.
authors.forename: Kanger
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D018925:Chemokines; D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha