title: Pure Mitochondrial DNA Does Not Activate Human Neutrophils in vitro.
abstract: Excessive activation of the innate immune system often leads to fatal consequences and can be considered as one of the phenoptotic events. After traumatic injury, various components of mitochondria are released into the circulation and stimulate myeloid cells of the innate immunity. Presumably, mitochondrial DNA (mtDNA) might activate immune cells (Zhang, Q., et al. (2010) Nature, 464, 104-107). In the present study, we investigated the role of mtDNA as a direct activator of human neutrophils, as well as a prognostic marker in patients with severe trauma. Quantitative determination of mtDNA in the plasma of these patients revealed its significant increase (p < 0.02) in the group of survivors compared to non-survivors. Highly purified mtDNA was not able to induce activation of human neutrophils, thus possibly indicating the existence of additional factor(s) ensuring the recognition of mtDNA as a damage-associated molecular pattern.
authors.affiliation: Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119991, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Prikhodko
authors.forename: A K
authors.initials: AK
authors.lastname: Shabanov
authors.forename: L A
authors.initials: LA
authors.lastname: Zinovkina
authors.forename: E N
authors.initials: EN
authors.lastname: Popova
authors.forename: M A
authors.initials: MA
authors.lastname: Aznauryan
authors.forename: N O
authors.initials: NO
authors.lastname: Lanina
authors.forename: M V
authors.initials: MV
authors.lastname: Vitushkina
authors.forename: R A
authors.initials: RA
authors.lastname: Zinovkin
mesh_terms: D015415:Biomarkers / Q000097:blood; D004272:DNA, Mitochondrial / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D015599:Trauma Severity Indices; D014947:Wounds and Injuries / Q000097:blood / Q000276:immunology
chemical_list: D015415:Biomarkers; D004272:DNA, Mitochondrial