title: Characterization of Shrimp Oil from Pandalus borealis by High Performance Liquid Chromatography and High Resolution Mass Spectrometry.
abstract: Northern shrimp (Pandalus borealis) oil, which is rich in omega-3 fatty acids, was recovered from the cooking water of shrimp processing facilities. The oil contains significant amounts of omega-3 fatty acids in triglyceride form, along with substantial long-chain monounsaturated fatty acids (MUFAs). It also features natural isomeric forms of astaxanthin, a nutritional carotenoid, which gives the oil a brilliant red color. As part of our efforts in developing value added products from waste streams of the seafood processing industry, we present in this paper a comprehensive characterization of the triacylglycerols (TAGs) and astaxanthin esters that predominate in the shrimp oil by using HPLC-HRMS and MS/MS, as well as 13C-NMR. This approach, in combination with FAME analysis, offers direct characterization of fatty acid molecules in their intact forms, including the distribution of regioisomers in TAGs. The information is important for the standardization and quality control, as well as for differentiation of composition features of shrimp oil, which could be sold as an ingredient in health supplements and functional foods.
authors.affiliation: Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. Guangling.Jiao@nrc-cnrc.gc.ca.|Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Guangling.Jiao@nrc-cnrc.gc.ca.
authors.forename: Guangling
authors.initials: G
authors.lastname: Jiao
authors.affiliation: Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Joseph.Hui@nrc-cnrc.gc.ca.
authors.forename: Joseph P M
authors.initials: JP
authors.lastname: Hui
authors.affiliation: Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Ian.Burton@nrc-cnrc.gc.ca.
authors.forename: Ian W
authors.initials: IW
authors.lastname: Burton
authors.affiliation: Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. marie-helene.thibault@umoncton.ca.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Thibault
authors.affiliation: Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. claude.pelletier@umoncton.ca.
authors.forename: Claude
authors.initials: C
authors.lastname: Pelletier
authors.affiliation: Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. josee.boudreau@umoncton.ca.
authors.forename: Josée
authors.initials: J
authors.lastname: Boudreau
authors.affiliation: Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. nadia.tchoukanova@irzc.umcs.ca.
authors.forename: Nadia
authors.initials: N
authors.lastname: Tchoukanova
authors.affiliation: Université de Moncton, Campus de Shippagan, 218, boulevard J.-D.-Gauthier, Shippagan, NB E8S 1P6, Canada. subramanian.balaji@umoncton.ca.
authors.forename: Balaji
authors.initials: B
authors.lastname: Subramanian
authors.affiliation: Université de Moncton, Campus de Shippagan, 218, boulevard J.-D.-Gauthier, Shippagan, NB E8S 1P6, Canada. yahia.djaoued@umoncton.ca.
authors.forename: Yahia
authors.initials: Y
authors.lastname: Djaoued
authors.affiliation: Novaceutics Consulting, 6501 Oak St., Halifax, NS B3L 1H5, Canada. stephen.ewart@novaceutics.ca.
authors.forename: Stephen
authors.initials: S
authors.lastname: Ewart
authors.affiliation: Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. jacques.gagnon@umoncton.ca.
authors.forename: Jacques
authors.initials: J
authors.lastname: Gagnon
authors.affiliation: Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. vewart@dal.ca.
authors.forename: Kathryn Vanya
authors.initials: KV
authors.lastname: Ewart
authors.affiliation: Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. junzeng.zhang@nrc-cnrc.gc.ca.
authors.forename: Junzeng
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009821:Oils / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D033542:Pandalidae / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014280:Triglycerides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D024341:Xanthophylls / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D015525:Fatty Acids, Omega-3; D009821:Oils; D014280:Triglycerides; D024341:Xanthophylls; C005948:astaxanthine
keywords: 13C-NMR; LC-HRMS and MS/MS; Pandalus borealis; astaxanthin fatty acids esters; fatty acid profile; shrimp oil; triacylglycerols