title: Daucus carota Pentane/Diethyl Ether Fraction Inhibits Motility and Reduces Invasion of Cancer Cells.
abstract: Daucus carota (DC) is a herb used in folklore medicine in Lebanon to treat numerous diseases including cancer. Recent studies in our laboratory on DC oil and its fractions revealed potent anticancer activities in vitro and in vivo. The present study aims to investigate the effect of the most potent DC fraction, pentane/diethyl ether (50:50), on lung, skin, breast and glioblastoma cancer cell motility and invasion. Upon treatment, a pronounced decrease in cancer cell motility was observed in the 4 cell lines. The treatment also led to a decrease in cancer cell invasion and an increased cell adhesion. Additionally, the DC fraction caused a decrease in the activation of the ρ-GTPases Rac and CDC42, a finding that may partially explain the treatment-induced decrease in cell motility. The current study demonstrates a crucial effect of the DC pentane/diethyl ether fraction on cancer cell motility and metastasis, making it a potential candidate for cancer therapy specifically targeting cancer motility and metastasis.
authors.affiliation: Department of Natural Sciences, Lebanese American University, Beirut, Lebanon.
authors.forename: Perla
authors.initials: P
authors.lastname: Zgheib
authors.forename: Costantine F
authors.initials: CF
authors.lastname: Daher
authors.forename: Mohamad
authors.initials: M
authors.lastname: Mroueh
authors.forename: Anita
authors.initials: A
authors.lastname: Nasrallah
authors.forename: Robin I
authors.initials: RI
authors.lastname: Taleb
authors.forename: Mirvat
authors.initials: M
authors.lastname: El-Sibai
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects*; D018552:Daucus carota; D004986:Ether / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D008546:Melanoma, Experimental; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000517:prevention & control*; D010420:Pentanes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves; D010938:Plant Oils / Q000302:isolation & purification / Q000627:therapeutic use; D012878:Skin Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: D010420:Pentanes; D010936:Plant Extracts; D010938:Plant Oils; D004986:Ether; C033353:pentane