title: Antiedematogenic Evaluation of Copaifera langsdorffii Leaves Hydroethanolic Extract and Its Major Compounds.
abstract: Inflammatory disorders affect many people worldwide, and medicinal plants are used to ameliorate these health problems. This paper reports the antiedematogenic and analgesic evaluation of Copaifera langsdorffii Desf. leaves hydroethanolic extract (Cop) and two of its isolated compounds: quercetin-3-O-α-l-rhamnopyranosyl (quercitrin) and kaempferol-3-O-α-l-rhamnopyranosyl (afzelin). For that, the following experimental protocols were undertaken locomotor performance, writhing induced by acetic acid, antinociceptivity induced by formalin, hot plate latency, paw oedema induced by carrageenan and dextran, and cell migration induced by lipopolysaccharide (LPS), as well as the measurement of nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 10 (IL-10) in macrophages. Neither the extract nor the isolated compounds displayed analgesic activity. The obtained results showed that C. langsdorffii extract possesses antiedematogenic properties acting on peripheral sites, whereas quercitrin and afzelin are not involved. Moreover, these properties are not associated with cell migration inhibition, TNF-α, IL-6, or IL-10 regulation.
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Ricardo Andrade
authors.identifier: 0000-0003-1828-9706
authors.initials: RA
authors.lastname: Furtado
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Cristiane Teixeira Vilhena
authors.identifier: 0000-0002-9251-4919
authors.initials: CT
authors.lastname: Bernardes
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Mauro Nogueira
authors.identifier: 0000-0002-8542-654X
authors.initials: MN
authors.lastname: da Silva
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Karina Furlani
authors.identifier: 0000-0002-9988-1009
authors.initials: KF
authors.lastname: Zoccal
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Lúcia Helena
authors.identifier: 0000-0002-4999-8305
authors.initials: LH
authors.lastname: Faccioli
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Jairo Kenupp
authors.identifier: 0000-0001-8641-9686
authors.initials: JK
authors.lastname: Bastos
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000633:toxicity; D002465:Cell Movement / Q000187:drug effects; D003911:Dextrans / Q000633:toxicity; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D007887:Fabaceae / Q000737:chemistry; D005557:Formaldehyde / Q000633:toxicity; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008362:Mannosides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D044945:Proanthocyanidins / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D011794:Quercetin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D003911:Dextrans; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008362:Mannosides; D010936:Plant Extracts; D044945:Proanthocyanidins; D014409:Tumor Necrosis Factor-alpha; D005557:Formaldehyde; C012526:quercitrin; C477954:afzelin; D002351:Carrageenan; D011794:Quercetin