title: In vivo characterization of the Drosophila mRNA 3' end processing core cleavage complex.
abstract: A core cleavage complex (CCC) consisting of CPSF73, CPSF100, and Symplekin is required for cotranscriptional 3' end processing of all metazoan pre-mRNAs, yet little is known about the in vivo molecular interactions within this complex. The CCC is a component of two distinct complexes, the cleavage/polyadenylation complex and the complex that processes nonpolyadenylated histone pre-mRNAs. RNAi-depletion of CCC factors in Drosophila culture cells causes reduction of CCC processing activity on histone mRNAs, resulting in read through transcription. In contrast, RNAi-depletion of factors only required for histone mRNA processing allows use of downstream cryptic polyadenylation signals to produce polyadenylated histone mRNAs. We used Dmel-2 tissue culture cells stably expressing tagged CCC components to determine that amino acids 272-1080 of Symplekin and the C-terminal approximately 200 amino acids of both CPSF73 and CPSF100 are required for efficient CCC formation in vivo. Additional experiments reveal that the C-terminal 241 amino acids of CPSF100 are sufficient for histone mRNA processing indicating that the first 524 amino acids of CPSF100 are dispensable for both CCC formation and histone mRNA 3' end processing. CCCs containing deletions of Symplekin lacking the first 271 amino acids resulted in dramatic increased use of downstream polyadenylation sites for histone mRNA 3' end processing similar to RNAi-depletion of histone-specific 3' end processing factors FLASH, SLBP, and U7 snRNA. We propose a model in which CCC formation is mediated by CPSF73, CPSF100, and Symplekin C-termini, and the N-terminal region of Symplekin facilitates cotranscriptional 3' end processing of histone mRNAs.
authors.affiliation: Department of Biology, University of Missouri-St. Louis, St. Louis, Missouri 63121, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Michalski
authors.affiliation: Department of Biology, University of Missouri-St. Louis, St. Louis, Missouri 63121, USA.
authors.forename: Mindy
authors.initials: M
authors.lastname: Steiniger
mesh_terms: D000818:Animals; D039223:Cleavage And Polyadenylation Specificity Factor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics; D009154:Mutation; D026723:Polyadenylation; D012333:RNA, Messenger / Q000378:metabolism*; D046509:Tissue Culture Techniques; D039221:mRNA Cleavage and Polyadenylation Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C000601911:CPSF100 protein, Drosophila; C000601910:CPSF73 protein, Drosophila; D039223:Cleavage And Polyadenylation Specificity Factor; D029721:Drosophila Proteins; D006657:Histones; D012333:RNA, Messenger; C541112:Sym protein, Drosophila; D039221:mRNA Cleavage and Polyadenylation Factors
keywords: CPSF100; CPSF73; Drosophila; Symplekin; cotranscriptional; mRNA 3′ end processing