title: Capillary electrophoretic studies on quantum dots and histidine appended peptides self-assembly.
abstract: Herein, we designed four peptides appended with different numbers of histidine (Hisn -peptide). We launched a systematic investigation on quantum dots (QDs) and Hisn -peptide self-assembly in solution using fluorescence coupled CE (CE-FL). The results indicated that CE-FL was a powerful method to probe how ligands interaction on the surface of nanoparticles. The self-assembly of QDs and peptide was determined by the numbers of histidine. We also observed that longer polyhistidine tags (n ≤ 6) could improve the self-assembly efficiency. Furthermore, the formation and separation of QD-peptide assembly were also studied by CE-FL inside a capillary. The total time for the mixing, self-assembly, separation, and detection was less than 10 min. Our method greatly expands the application of CE-FL in QDs-based biolabeling and bioanalysis.
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Yiwan
authors.initials: Y
authors.lastname: Teng
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Cheli
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jinchen
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Dong
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006639:Histidine / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D010455:Peptides; C033223:polyhistidine; D006639:Histidine
keywords: CE-FL; Hisn-peptide; In-capillary; Quantum dots; Self-assembly