title: Phloem-exudate proteome analysis of response to insect brown plant-hopper in rice.
abstract: Brown plant-hopper (Nilaparvata lugens Stål, BPH), one of the most devastating agricultural insect pests of rice throughout Asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. Planting resistant variety is an efficient and economical way to control this pest. Understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. Here, we used an iTRAQ-based quantitative proteomics approach to perform analysis of protein expression profiles in the phloem exudates of BPH-resistant and susceptible rice plants following BPH infestation. A total of 238 proteins were identified, most of which were previously described to be present in the phloem of rice and other plants. The expression of genes for selected proteins was confirmed using a laser capture micro-dissection method and RT-PCR. The mRNAs for three proteins, RGAP, TCTP, and TRXH, were further analyzed by using in situ mRNA hybridization and localized in the phloem cells. Our results showed that BPH feeding induced significant changes in the abundance of proteins in phloem sap of rice involved in multiple pathways, including defense signal transduction, redox regulation, and carbohydrate and protein metabolism, as well as cell structural proteins. The results presented provide new insights into rice resistance mechanisms and should facilitate the breeding of novel elite BPH-resistant rice varieties.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Ba
authors.initials: B
authors.lastname: Du
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Zhanqi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Du
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: gche@whu.edu.cn.
authors.forename: Guangcun
authors.initials: G
authors.lastname: He
mesh_terms: D000818:Animals; D004492:Edetic Acid / Q000737:chemistry; D005122:Exudates and Transudates / Q000737:chemistry; D020387:Food Chain; D006430:Hemiptera / Q000254:growth & development / Q000502:physiology*; D060434:Herbivory; D009758:Nymph / Q000254:growth & development / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D052585:Phloem / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020543:Proteome; D004492:Edetic Acid
keywords: Nilaparvata lugens; Oryza sativa; Plant-insect interactions