title: Enhanced Production of Polysaccharide Through the Overexpression of Homologous Uridine Diphosphate Glucose Pyrophosphorylase Gene in a Submerged Culture of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes).
abstract: This study aimed to improve polysaccharide production by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of the homologous UDP glucose pyrophosphorylase (UGP) gene. The effects of UGP gene overexpression on intracellular polysaccharide (IPS) content, extracellular polysaccharide (EPS) production, and transcription levels of 3 genes encoding the enzymes involved in polysaccharide biosynthesis, including phosphoglucomutase (PGM), UGP, and α-1,3-glucan synthase (GLS), were investigated. The maximum IPS content and EPS production in G. lucidum overexpressing the UGP gene were 24.32 mg/100 mg dry weight and 1.66 g/L, respectively, which were higher by 42% and 36% than those of the wild-type strain. The transcription levels of PGM, UGP, and GLS were up-regulated by 1.6, 2.6, and 2.4-fold, respectively, in the engineered strain, suggesting that increased polysaccharide biosynthesis may result from a higher expression of those genes.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Sen-Lin
authors.initials: SL
authors.lastname: Ji
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Meng-Fei
authors.initials: MF
authors.lastname: Ren
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
authors.forename: Huan-Jun
authors.initials: HJ
authors.lastname: Li
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Xu
mesh_terms: D015966:Gene Expression Regulation, Fungal; D008519:Medicine, Traditional; D010802:Phylogeny; D011134:Polysaccharides / Q000096:biosynthesis*; D020940:Reishi / Q000201:enzymology* / Q000235:genetics; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D011134:Polysaccharides; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase