title: Improvement and modeling of culture parameters to enhance biomass and lipid production by the oleaginous yeast Cryptococcus curvatus grown on acetate.
abstract: The improvement of culture parameters for lipid production from acetate as carbon source was investigated using the oleaginous yeast Cryptococcus curvatus. A new pH regulation system dispensing acetate was developed for fed-batch culture and allowed obtaining nearly 80 g/L biomass within 60 h with a maximal growth rate of 0.28 h(-1). A biological model was developed from experimental data. The influence of three C/N ratios of 300, 500 and 900 were tested during a multi-phases process on lipid accumulation. The C/N ratio of 300 was reported to be the most suitable for lipid storage. No significant increase of lipids content was obtained with higher value. A maximal content of 60% DCW of lipid was obtained. The determination of fatty acids profiles of the microbial oils has confirmed that the valorization of acetate by microbial oils production was a promising perspective.
authors.affiliation: Université Blaise Pascal, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubiere cedex, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Béligon
authors.affiliation: Université Blaise Pascal, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubiere cedex, France; Université Blaise Pascal, LABEX IMobS3, TSA 60026, CS 60026, F-63178 Aubiere cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poughon
authors.affiliation: Université Blaise Pascal, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubiere cedex, France; Université Blaise Pascal, LABEX IMobS3, TSA 60026, CS 60026, F-63178 Aubiere cedex, France.
authors.forename: Gwendoline
authors.initials: G
authors.lastname: Christophe
authors.affiliation: Université Blaise Pascal, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubiere cedex, France; Université Blaise Pascal, LABEX IMobS3, TSA 60026, CS 60026, F-63178 Aubiere cedex, France.
authors.forename: André
authors.initials: A
authors.lastname: Lebert
authors.affiliation: Université Blaise Pascal, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubiere cedex, France; Université Blaise Pascal, LABEX IMobS3, TSA 60026, CS 60026, F-63178 Aubiere cedex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Larroche
authors.affiliation: Université Blaise Pascal, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubiere cedex, France; Université Blaise Pascal, LABEX IMobS3, TSA 60026, CS 60026, F-63178 Aubiere cedex, France. Electronic address: Pierre.FONTANILLE@univ-bpclermont.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Fontanille
mesh_terms: D000085:Acetates / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000502:physiology; D003198:Computer Simulation; D003454:Cryptococcus / Q000737:chemistry / Q000145:classification / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000302:isolation & purification; D008954:Models, Biological
chemical_list: D000085:Acetates; D003470:Culture Media; D008055:Lipids
keywords: Bioconversion; Biodiesel; Cryptococcus curvatus; Microbial lipids; Volatile fatty acids