title: Proteomic analysis of 3-MCPD and 3-MCPD dipalmitate toxicity in rat testis.
abstract: Thermal treatment of foodstuff containing fats and salt promotes the formation of 3-chloropropane-1,2-diol (3-MCPD) and its fatty acid esters. 3-MCPD-exposed rats develop testicular lesions and Leydig cell tumors. 3-MCPD and 3-MCPD ester toxicity is thought to be caused by 3-MCPD and its metabolites, since 3-MCPD esters are hydrolyzed in the gut. Inhibition of glycolysis is one of the few known molecular mechanisms of 3-MCPD toxicity. To obtain deeper insight into this process, a comparative proteomic approach was chosen, based on a 28-days repeated-dose feeding study with male Wistar rats. Animals received equimolar doses of 3-MCPD or 3-MCPD dipalmitate. A lower dose of 3-MCPD dipalmitate was also administered. Absence of histopathological changes supported an analysis of early cellular disturbance. Testes were analyzed by two-dimensional gel electrophoresis followed by mass-spectrometric protein identification. Data provide a comprehensive overview of proteomic changes induced by 3-MCPD and 3-MCPD dipalmitate in rat testis in an early phase of organ impairment. Results are compatible with known 3-MCPD effects on reproductive function, substantially extend our knowledge about cellular responses to 3-MCPD and support the hypothesis that toxicity of 3-MCPD and 3-MCPD esters is mediated via common effectors. DJ-1 was identified as a candidate marker for 3-MCPD exposure.
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Sawada
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Oberemm
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Buhrke
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Meckert
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
authors.forename: Christel
authors.initials: C
authors.lastname: Rozycki
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany. Electronic address: Albert.Braeuning@bfr.bund.de.
authors.forename: Albert
authors.initials: A
authors.lastname: Braeuning
authors.affiliation: Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Lampen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination; D005511:Food Handling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006358:Hot Temperature / Q000009:adverse effects; D008297:Male; D008869:Microtubule-Associated Proteins / Q000819:agonists / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000819:agonists / Q000096:biosynthesis; D010168:Palmitates / Q000008:administration & dosage / Q000633:toxicity*; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D000071617:Protein Deglycase DJ-1; D040901:Proteomics / Q000379:methods; D011897:Random Allocation; D017208:Rats, Wistar; D013736:Testicular Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects; D000517:alpha-Chlorohydrin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010168:Palmitates; D000517:alpha-Chlorohydrin; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1
keywords: 3-Chloro-1,2-monopropanediol; Food carcinogen; Infertility; Protein DJ-1/PARK7; Testicular carcinogenicity