title: Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium.
abstract: In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment.
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: amir.abdulmawjood@tiho-hannover.de.
authors.forename: A
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Wickhorst
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: SAC Consulting Veterinary Services, Drummondhill, Stratherrick Road, Inverness IV2 4JZ, UK.
authors.forename: G
authors.initials: G
authors.lastname: Foster
authors.affiliation: Animal Health and Veterinary Laboratories Agency, Penrith Regional Laboratory, Merrythought, Calthwaite, Penrith CA11 9RR, UK.
authors.forename: P N
authors.initials: PN
authors.lastname: Wragg
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Frankfurterstr. 85-91, 35392 Gießen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Klein
mesh_terms: D055667:Arcanobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial
keywords: Arcanobacterium pluranimalium; Loop-mediated isothermal amplification; Pluranimaliumlysin; pla