title: Mobile genetic elements related to the diffusion of plasmid-mediated AmpC β-lactamases or carbapenemases from Enterobacteriaceae: findings from a multicenter study in Spain.
abstract: We examined the genetic context of 74 acquired ampC genes and 17 carbapenemase genes from 85 of 640 Enterobacteriaceae isolates collected in 2009. Using S1 pulsed-field gel electrophoresis and Southern hybridization, 37 of 74 bla AmpC genes were located on large plasmids of different sizes belonging to six incompatibility groups. We used sequencing and PCR mapping to investigate the regions flanking the acquired ampC genes. The bla CMY-2-like genes were associated with ISEcp1; the surrounding bla DHA genes were similar to Klebsiella pneumoniae plasmid pTN60013 associated with IS26 and the psp and sap operons; and the bla ACC-1 genes were associated with IS26 elements inserted into ISEcp1. All of the carbapenemase genes (bla VIM-1, bla IMP-22, and bla IMP-28) were located in class 1 integrons. Therefore, although plasmids are the main cause of the rapid dissemination of ampC genes among Enterobacteriaceae, we need to be aware that other mobile genetic elements, such as insertion sequences, transposons, or integrons, can be involved in the mobilization of these genes of chromosomal origin. Additionally, three new integrons (In846 to In848) are described in this study.
authors.affiliation: Servicio de Microbiología and Unidad de Investigación, Hospital Universitario Son Espases, Instituto de Investigación Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Zamorano
authors.affiliation: Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Miró
authors.affiliation: Servicio de Microbiología and Unidad de Investigación, Hospital Universitario Son Espases, Instituto de Investigación Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Juan
authors.affiliation: Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Gómez
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario a Coruña, A Coruña, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Bou
authors.affiliation: Servei de Microbiologia, Hospital Vall d'Hebrón, Barcelona, Spain Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: González-López
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla-IDIVAL, and Departamento de Biología Molecular, Universidad de Cantabria, Cantabria, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Martínez
authors.affiliation: Laboratorio de Antibióticos, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Aracil
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Sevilla, Sevilla, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Conejo
authors.affiliation: Servicio de Microbiología and Unidad de Investigación, Hospital Universitario Son Espases, Instituto de Investigación Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Oliver
authors.affiliation: Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain fnavarror@santpau.cat.
authors.forename: F
authors.identifier: http://orcid.org/0000-0002-4302-2838
authors.initials: F
authors.lastname: Navarro
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology*; D032023:Integrons / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D013030:Spain; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C063912:carbapenemase