title: Activation of insulin-like growth factor 1 receptor regulates the radiation-induced lung cancer cell apoptosis.
abstract: OBJECTIVE
  The prevalence of lung cancer is increasing in the recent decades. The underlying mechanism is unclear. The insulin-like growth factor (IGF) and p53 protein are important molecules involving the tumor immunity. This study aims to investigate the role of IGF intervene the radiation-induced lung cancer apoptosis.
  
  
  METHODS
  Lung cancer cells were isolated from surgically removed lung cancer tissue. The lung cancer cell lines, A549 cells and H23 cells were irradiated. The expression of IGF1 receptor (IGF1R) by the lung cancer cells, and apoptosis, were assessed by flow cytometry.
  
  
  RESULTS
  The results showed that human lung cancer cells expressed IGF1R. IGF1R played a critical role in the radiation-induced lung cancer cell apoptosis. The histone deacetylase-1 (HDAC1) phosphorylation was up regulated by irradiation. The phosphorylated HDAC1 bound the p53 promoter to inhibit the gene transcription, which was abolished by the presence of an inhibitor of HDAC1 or a STAT3 inhibitor.
  
  
  CONCLUSIONS
  The data suggest that activation of IGF1R plays a critical role in the radioresistance, which can be prevented in the presence of the inhibitors of HDAC1 or STAT3 inhibitors.
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital of Central South University, No.87, Xiangya Road, Kaifu District, HuNan, ChangSha 410008, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital of Central South University, No.87, Xiangya Road, Kaifu District, HuNan, ChangSha 410008, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital of Central South University, No.87, Xiangya Road, Kaifu District, HuNan, ChangSha 410008, China. Electronic address: dongkairwu@outlook.com.
authors.forename: Dongkai
authors.initials: D
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D045744:Cell Line, Tumor; D005720:Gamma Rays; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D056284:Histone Deacetylase 1 / Q000037:antagonists & inhibitors / Q000276:immunology; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000276:immunology*; D011836:Radiation Tolerance / Q000187:drug effects / Q000276:immunology*; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000276:immunology*; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000276:immunology
chemical_list: D056572:Histone Deacetylase Inhibitors; C000597236:IGF1R protein, human; D009363:Neoplasm Proteins; D017451:Receptors, Somatomedin; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D017526:Receptor, IGF Type 1; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1
keywords: Cander; Histone deacetylase-1; Insulin-like growth factor; Lung; Radiotherapy