title: Single cell analysis using a glass microchamber for studying movement fluctuations of Navicula pavillardii and Seminavis robusta diatom cells.
abstract: We propose a new technique for quantitative trajectory analysis of gliding phenomenon of Navicula pavillardii (NP) and Seminavis robusta (SR) diatom cells by single cell observation using a glass microchamber in this short technical note. Two-dimensional trajectory analysis of cell movements was used to determine the angular velocity, velocity, and migration distances of the diatom movement. Based on the trajectory analysis, we found that asymmetrically shaped SR had a larger angular velocity with large fluctuations compared to symmetrically shaped NP, although the velocity of SR was less than that of NP. It suggests that lateral frictional force in a culture medium is an important factor for diatom movements. Our results revealed that the single cell observation using a glass microchamber is effective on quantitative analysis of angular velocity of diatom gliding.
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan. Electronic address: meicun2006@163.com.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sadoya
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nagao
authors.affiliation: Graduate School of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Oikawa
authors.affiliation: Graduate School of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan; RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hanada
authors.affiliation: RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Sugioka
authors.affiliation: Faculty of Education, Tokyo Gakugei University, 4-1-1 Nukui-kita-machi, Koganei, Tokyo 184-8511, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Mayama
mesh_terms: D017377:Diatoms / Q000502:physiology* / Q000648:ultrastructure; D005898:Glass; D009068:Movement; D059010:Single-Cell Analysis / Q000379:methods*
keywords: Angular velocity; Diatom; Gliding; Microchamber; Trajectory