title: Evaluation of surfactants on waste activated sludge fermentation by pyrosequencing analysis.
abstract: The effects of three widely-used surfactants on waste activated sludge (WAS) fermentation and microbial community structures were investigated. Rhamnolipid bio-surfactants (RL) showed more positive effects on WAS hydrolysis and acidification compared to chemosynthetic surfactants, such as sodium dodecylsulphate (SDS) and sodium dodecyl benzene sulfonate (SDBS). The highest SCOD and VFAs concentrations obtained with RL were 1.15-fold and 1.16-fold that of SDS, and up to 1.73 and 3.63 times higher than those obtained with SDBS. Pyrosequencing analysis showed that an evident reduction in bacterial diversity in surfactant-treated WAS. Moreover, acid-producing bacteria (such as Megasphaera and Oscillibacter), detected with RL, were (6.8% and 6.4% in proportion) more abundant than with SDS, and were rarely found in SDBS and the control. The results also revealed that RL allowed efficient hydrolysis enhancement and was favorable to functional microorganisms for further acidification during WAS fermentation.
authors.affiliation: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, China.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Zhou
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wenzong
authors.initials: W
authors.lastname: Liu
authors.affiliation: Section for Sustainable Biotechnology, Aalborg University Copenhagen, Denmark.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Varrone
authors.affiliation: School of Mechanical Engineering and Automation, Northeastern University, Shenyang, China.
authors.forename: Youzhao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China.
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.affiliation: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, China. Electronic address: yuexiuping1990@126.com.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Yue
mesh_terms: D001483:Base Sequence; D005285:Fermentation; D000068536:Bacillota / Q000302:isolation & purification / Q000502:physiology*; D016680:Genome, Bacterial / Q000235:genetics; D045851:Megasphaera / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D012037:Refuse Disposal / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology*; D013501:Surface-Active Agents
chemical_list: D012722:Sewage; D013501:Surface-Active Agents
keywords: Anaerobic digestion; Microbial community; Pyrosequencing; Surfactant; Waste activated sludge (WAS)