title: Effect of process variables on the sulfate reduction process in bioreactors treating metal-containing wastewaters: factorial design and response surface analyses.
abstract: The individual and combined effect of the pH, chemical oxygen demand (COD) and SO4 (2-) concentration, metal to sulfide (M/S(2-)) ratio and hydraulic retention time (HRT) on the biological sulfate reduction (SR) process was evaluated in an inverse fluidized bed reactor by factorial design analysis (FDA) and response surface analysis (RSA). The regression-based model of the FDA described the experimental results well and revealed that the most significant variable affecting the process was the pH. The combined effect of the pH and HRT was barely observable, while the pH and COD concentration positive effect (up to 7 and 3 gCOD/L, respectively) enhanced the SR process. Contrary, the individual COD concentration effect only enhanced the COD removal efficiency, suggesting changes in the microbial pathway. The RSA showed that the M/S(2-) ratio determined whether the inhibition mechanism to the SR process was due to the presence of free metals or precipitated metal sulfides.
authors.affiliation: Chair Group Pollution Prevention and Resource Recovery, UNESCO-IHE, Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands, denyskristalia@hotmail.com.
authors.forename: D K
authors.initials: DK
authors.lastname: Villa-Gomez
authors.forename: K
authors.initials: K
authors.lastname: Pakshirajan
authors.forename: R
authors.initials: R
authors.lastname: Maestro
authors.forename: S
authors.initials: S
authors.lastname: Mushi
authors.forename: P N L
authors.initials: PN
authors.lastname: Lens
mesh_terms: D001673:Biodegradation, Environmental; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D003627:Data Interpretation, Statistical; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000737:chemistry*; D010084:Oxidation-Reduction; D013431:Sulfates / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry; D016999:Sulfur-Reducing Bacteria / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D019216:Metals, Heavy; D013431:Sulfates; D013440:Sulfides; D062065:Wastewater; D014874:Water Pollutants, Chemical