title: Honey shows potent inhibitory activity against the bovine testes hyaluronidase.
abstract: The purpose of this study was to investigate the anti-hyaluronidase activities of honeys from different botanical origins honeys in order to determine their anti-inflammatory properties. The total phenolic contents, total flavonoids and total tannin levels of six types of honey, chestnut, oak, heather, pine, buckwheat and mixed blossom, were determined. Concentration-related inhibition values were tested turbidimetrically on bovine testis hyaluronidase (BTHase) as IC50 (mg/mL). All honeys exhibited various concentration-dependent degrees of inhibition against BTHase. Inhibition values varied significantly depending on honeys' levels of phenolic contents, flavonoid and tannin. The honeys with the highest anti-hyaluronidase activity were oak, chestnut and heather. In conclusion, polyphenol-rich honeys have high anti-hyaluronidase activity, and these honeys have high protective and complementary potential against hyaluronidase-induced anti-inflammatory failures.
authors.affiliation: a Department of Chemistry, Faculty of Science , Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kolayli
authors.affiliation: b Espiye Vocational School, Giresun University , Espiye , Giresun , Turkey .
authors.forename: Huseyin
authors.initials: H
authors.lastname: Sahin
authors.affiliation: c Şebinkarahisar Technical Sciences Vocational School, Giresun University , Giresun , Turkey , and.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Can
authors.affiliation: d Maçka Vocational School, Karadeniz Technical University , Maçka , Trabzon , Turkey.
authors.forename: Oktay
authors.initials: O
authors.lastname: Yildiz
authors.affiliation: a Department of Chemistry, Faculty of Science , Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Kübra
authors.initials: K
authors.lastname: Sahin
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006722:Honey; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013737:Testis / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D059808:Polyphenols; D006821:Hyaluronoglucosaminidase
keywords: Flavonoid; hyaluronidase; inhibition; phenolic content; tannin