title: Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.
abstract: The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.
authors.affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Delgado-González
authors.affiliation: Radiology Service, Magnetic Resonance Unit, Complejo Hospitalario Universitario de Albacete (CHUA), Albacete, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Mansilla-Legorburo
authors.affiliation: Radiodiagnostic Service, Hospital Nacional de Parapléjicos (HNP), Toledo, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Florensa-Vila
authors.affiliation: Department of Health, Physical Therapy School, Public University of Navarra, Tudela, Spain.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Insausti
authors.affiliation: School of Advanced Education, Research and Accreditation, Castellón de la Plana, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Viñuela
authors.affiliation: Pathology Service, Hospital Complex of Navarre, Pamplona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Tuñón-Alvarez
authors.affiliation: Department of Mathematics, Statistics and Computation, University of Cantabria, Santander, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: Cruz
authors.affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Mohedano-Moriano
authors.affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Insausti
authors.affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Artacho-Pérula
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001931:Brain Mapping / Q000379:methods; D016022:Case-Control Studies; D003337:Corpus Callosum / Q000473:pathology; D005260:Female; D006624:Hippocampus / Q000473:pathology*; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D008875:Middle Aged; D019636:Neurodegenerative Diseases / Q000473:pathology; D013702:Temporal Lobe / Q000473:pathology*