title: Bifidobacterium breve alters immune function and ameliorates DSS-induced inflammation in weanling rats.
abstract: BACKGROUND
  Bifidobacterium breve M-16V (M16V) is a probiotic bacterial strain with a long tradition of use in neonatal intensive care units in some countries. Previous study showed that the effects of M16V administration on gene expression were greater during the weaning period than in the neonatal period and were greater in the colon than in the small intestine and spleen, suggesting that M16V has anti-inflammatory effects. In this study, we evaluated the effects of inflammation during the weaning period and the effects of M16V on normal and inflammatory conditions.
  
  
  METHODS
  From postnatal day (PD) 21 to 34, weanling rats were administered of 2.5 × 10(9) of M16V daily, and colitis was induced by administration of 2% dextran sulfate sodium from PD28 to 35. Colitis severity, immune function, and microbiota were investigated.
  
  
  RESULTS
  Colitis caused a reduction in body weight gain, colon shortening, poor nutritional status, anemia, changes in blood and spleen lymphocyte populations, spleen T-cell malfunctions, and alterations in colon microbiota. M16V administration improved some but not all of the changes induced by colitis.
  
  
  CONCLUSIONS
  M16V could suppress inflammation and, therefore, can be considered a safe strain to use not only during the neonatal period but also the weaning period.
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co., Ltd., Higashihara, Zama-City, Kanagawa-Pref, Japan.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Izumi
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co., Ltd., Higashihara, Zama-City, Kanagawa-Pref, Japan.
authors.forename: Mario
authors.initials: M
authors.lastname: Minegishi
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co., Ltd., Higashihara, Zama-City, Kanagawa-Pref, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co., Ltd., Higashihara, Zama-City, Kanagawa-Pref, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co., Ltd., Higashihara, Zama-City, Kanagawa-Pref, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sekine
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co., Ltd., Higashihara, Zama-City, Kanagawa-Pref, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Takase
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn; D001644:Bifidobacterium / Q000276:immunology*; D002478:Cells, Cultured; D003092:Colitis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D009752:Nutritional Status; D019936:Probiotics; D011916:Rats, Inbred F344; D012720:Severity of Illness Index; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000382:microbiology; D013997:Time Factors; D014886:Weaning
chemical_list: D016264:Dextran Sulfate