title: Linkage of an ABCC transporter to a single QTL that controls Ostrinia nubilalis larval resistance to the Bacillus thuringiensis Cry1Fa toxin.
abstract: Field evolved resistance of insect populations to Bacillus thuringiensis (Bt) crystalline (Cry) toxins expressed by crop plants has resulted in reduced control of insect feeding damage to field crops, and threatens the sustainability of Bt transgenic technologies. A single quantitative trait locus (QTL) that determines resistance in Ostrinia nubilalis larvae capable of surviving on reproductive stage transgenic corn that express the Bt Cry1Fa toxin was previously mapped to linkage group 12 (LG12) in a backcross pedigree. Fine mapping with high-throughput single nucleotide polymorphism (SNP) anchor markers, a candidate ABC transporter (abcc2) marker, and de novo mutations predicted from a genotyping-by-sequencing (GBS) data redefined a 268.8 cM LG12. The single QTL on LG12 spanned an approximate 46.1 cM region, in which marker 02302.286 and abcc2 were ≤ 2.81 cM, and the GBS marker 697 was an estimated 1.89 cM distant from the causal genetic factor. This positional mapping data showed that an O. nubilalis genome region encoding an abcc2 transporter is in proximity to a single QTL involved in the inheritance of Cry1F resistance, and will assist in the future identification the mutation(s) involved with this phenotype.
authors.affiliation: USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, IA 50011, USA; Department of Entomology, Iowa State University, Ames, IA 50011, USA. Electronic address: brad.coates@ars.usda.gov.
authors.forename: Brad S
authors.initials: BS
authors.lastname: Coates
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE, USA.
authors.forename: Blair D
authors.initials: BD
authors.lastname: Siegfried
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics; D004731:Endotoxins / Q000235:genetics; D008040:Genetic Linkage; D007814:Larva / Q000235:genetics; D009036:Moths / Q000235:genetics*; D009154:Mutation; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D003313:Zea mays / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004731:Endotoxins
keywords: Crystalline (Cry) toxin resistance; Genotyping by sequencing; QTL mapping