title: Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana.
abstract: The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani. HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes.
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.
authors.forename: V C
authors.initials: VC
authors.lastname: Beracochea
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.
authors.forename: N I
authors.initials: NI
authors.lastname: Almasia
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Peluffo
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.
authors.forename: V
authors.initials: V
authors.lastname: Nahirñak
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.|Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (EHA1428), Buenos Aires, Argentina.
authors.forename: E H
authors.initials: EH
authors.lastname: Hopp
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma De Buenos Aires, Buenos Aires, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Paniego
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.|Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (EHA1428), Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma De Buenos Aires, Buenos Aires, Argentina.
authors.forename: R A
authors.initials: RA
authors.lastname: Heinz
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma De Buenos Aires, Buenos Aires, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Vazquez-Rovere
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina. lia.veronica@inta.gob.ar.|Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (EHA1428), Buenos Aires, Argentina. lia.veronica@inta.gob.ar.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma De Buenos Aires, Buenos Aires, Argentina. lia.veronica@inta.gob.ar.
authors.forename: V V
authors.initials: VV
authors.lastname: Lia
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D001203:Ascomycota / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006368:Helianthus / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C058814:germin
keywords: Arabidopsis thaliana; Germin-like protein; ROS accumulation; Rhizoctonia solani; Sclerotinia sclerotiorum; Sunflower