title: A pilot study of the efficacy of wipes containing chlorhexidine 0.3%, climbazole 0.5% and Tris-EDTA to reduce Malassezia pachydermatis populations on canine skin.
abstract: BACKGROUND
  Wipes containing chlorhexidine and azole derivates have been recommended for veterinary use. No study has been published about their activity against Malassezia pachydermatis.
  
  
  OBJECTIVE
  To evaluate the in vivo and in vitro activity of wipes soaked in a chlorhexidine, climbazole and Tris-EDTA solution against Malassezia pachydermatis.
  
  
  METHODS
  Five research colony shar-pei dogs.
  
  
  METHODS
  Wipes were applied once daily onto the left axilla, left groin and perianal area (protocol A), and twice daily on the right axilla, right groin and umbilical region (protocol B) for 3 days. In vivo activity was evaluated by quantifying Malassezia colonies through contact plates on the selected body areas before and after wipe application. The activity of the solution in which the wipes were soaked was assessed in vitro by contact tests following the European Standard UNI EN 1275 guidelines.
  
  
  RESULTS
  Samples collected after wipe application showed a significant and rapid reduction of Malassezia yeast CFU. No significant difference in the Malassezia reduction was found between protocols A and B. In vitro assay showed 100% activity against Malassezia yeasts after a 15 min contact time with the wipe solution.
  
  
  CONCLUSIONS
  Wipes containing chlorhexidine, climbazole and Tris-EDTA substantially reduced the M. pachydermatis population on the skin of dogs. The results, although this was an uncontrolled study performed on a small number of dogs, suggest that these wipes may be useful for topical therapy of Malassezia dermatitis involving the lips, paws, perianal area and skin folds.
authors.affiliation: Department of Parasitology, Mycology and Dermatology, Dynamic Research Group, Ecole Nationale Vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Paola
authors.initials: P
authors.lastname: Cavana
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095, Grugliasco, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Peano
authors.affiliation: Institut de Recherche Clinique d'Alfort, Ecole Nationale Vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Jean-Yanique
authors.initials: JY
authors.lastname: Petit
authors.affiliation: Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095, Grugliasco, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Tizzani
authors.affiliation: Institut de Recherche Clinique d'Alfort, Ecole Nationale Vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrot
authors.affiliation: Dermatology Referral Service, 6 rue Mare Pavée, 35510, Rennes-Cesson, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bensignor
authors.affiliation: Department of Parasitology, Mycology and Dermatology, Dynamic Research Group, Ecole Nationale Vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Guillot
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use*; D003881:Dermatomycoses / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000188:drug therapy* / Q000382:microbiology; D004285:Dogs; D004359:Drug Therapy, Combination / Q000662:veterinary; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D007093:Imidazoles / Q000008:administration & dosage / Q000627:therapeutic use*; D008290:Malassezia; D008297:Male; D010865:Pilot Projects
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; D004492:Edetic Acid; C108286:climbazole; D002710:Chlorhexidine