title: Advances in proteomics for production strain analysis.
abstract: Proteomics is the large-scale study and analysis of proteins, directed to analysing protein function in a cellular context. Since the vast majority of the processes occurring in a living cell rely on protein activity, proteomics offer a unique vantage point from which researchers can dissect, characterise, understand and manipulate biological systems. When developing a production strain, proteomics offers a versatile toolkit of analytical techniques. In this commentary, we highlight a number of recent developments in this field using three industrially relevant case studies: targeted proteomic analysis of heterologous pathways in Escherichia coli, biofuel production in Synechocystis PCC6803 and proteomic investigations of lignocellulose degradation. We conclude by discussing future developments in proteomics that will impact upon metabolic engineering and process monitoring of bio-producer strains.
authors.affiliation: Department of Chemical and Biological Engineering, University of Sheffield, S1 3JD, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Landels
authors.affiliation: Department of Chemical and Biological Engineering, University of Sheffield, S1 3JD, UK.
authors.forename: Caroline
authors.initials: C
authors.lastname: Evans
authors.affiliation: Chaire de bioinformatique, Laboratory 'Genomics, Bioinformatics and Applications', Conservatoire national des arts et métiers, 75003 Paris, France.
authors.forename: Josselin
authors.initials: J
authors.lastname: Noirel
authors.affiliation: Department of Chemical and Biological Engineering, University of Sheffield, S1 3JD, UK. Electronic address: p.c.wright@sheffield.ac.uk.
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Wright
mesh_terms: D056804:Biofuels; D006801:Humans; D008031:Lignin / Q000378:metabolism; D060847:Metabolic Engineering; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D056804:Biofuels; D011506:Proteins; C036909:lignocellulose; D008031:Lignin