title: HK2 is a radiation resistant and independent negative prognostic factor for patients with locally advanced cervical squamous cell carcinoma.
abstract: The mechanism by which overexpression of hexokinase 2 (HK2) indicates locally advanced cervical squamous cell carcinoma (LACSCC) with radio-resistance is still unknown despite being an independent biomarker of poor prognosis. Here, we retrospectively analyzed 132 female patients receiving radiotherapy for cervical squamous cell carcinoma including 85 radiation-sensitive cases and 47 radiation-resistant cases. The expression of HK2 was examined by immunohistochemistry. The percentage of high HK2 expression in the radiation-resistant group differed from the radiation-sensitive group with statistical significance (P < 0.001) even if divided into three subgroups including a lower 5-year progression free survival group (PFS) for comparison (P < 0.001). The Kaplan Meier curve analysis showed that there were differences between the two groups (P < 0.001). Therefore, this study proves a close relationship between HK2 expression and radio-resistance. Multivariate Cox regression analysis implied that HK2 was an independent prognostic indicator of cervical squamous carcinoma (HR (95% CI), 2.940 (1.609, 1.609); P = 0.002).
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Xinqiong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Hunan Provincial Tumal Hospital & Affiliated Tumor Hospital of Xiangya Medical School, Central South University Hunan Province, P.R. China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Youyi
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University Hunan Province, P.R. China.
authors.forename: Liangfang
authors.initials: L
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D001918:Brachytherapy / Q000009:adverse effects / Q000401:mortality; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy*; D016009:Chi-Square Distribution; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D006593:Hexokinase / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D015999:Multivariate Analysis; D016016:Proportional Hazards Models; D011836:Radiation Tolerance; D012189:Retrospective Studies; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy*
chemical_list: D014408:Biomarkers, Tumor; C000609700:HK2 protein, human; D006593:Hexokinase
keywords: HK2; Locally advanced cervical squamous cell carcinoma; glycolysis; immunohistochemistry; radiation resistance