title: [Value of cervical liquid-based cytology and high-risk human papilloma virus for patients of high-grade squamous intraepithelial lesion excised with positive margins by loop electrosurgical excision procedure].
abstract: OBJECTIVE
  To explore the value of cervical liquid-based cytology test (LCT) and high-risk human papilloma virus for patients of high-grade squamous intraepithelial lesion (HSIL) excised with positive margins by loop electrosurgical excision procedure (LEEP).
  
  
  METHODS
  A total of 404 HSIL patients with positive margins by LEEP and a follow-up of 12 months between June 2004 to April 2013 were recruited. The results of LCT, high-risk human papillomavirus (HR-HPV) DNA were analyzed retrospectively. According to the pathological diagnosis of re-operation, the results of LCT and HR-HPV DNA were analytically predicted for focal residue and recurrence.
  
  
  RESULTS
  For patients with HSIL excised with positive margins by LEEP, the re-positive abnormality rate of LCT and/or HPV was 18.7% and the sustained positive rate of LCT and/or HPV 51.1%. The significance prediction of LCT and HR-HPV DNA examination for persistent and recurrent cervical dysplasia had a positive predictive value of 60%, a sensitivity of 100%, a positive likelihood ratio of 2.1, a negative likelihood ratio of 0.5 and an odds ratio of 4.5.
  
  
  CONCLUSIONS
  For patients of HSIL excised with positive margins by loop electrosurgical excision procedure, LCT plus HR-HPV test may be effective after operation. And the positive results should be re-evaluated by colposcopy to provide rationales for re-operation.
authors.affiliation: Cervical Department, International Peace Maternity & Child Health Hospital of China Welfare Institute, Shanghai 200030, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Cervical Department, International Peace Maternity & Child Health Hospital of China Welfare Institute, Shanghai 200030, China; Email: dr.wudan@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Cervical Department, International Peace Maternity & Child Health Hospital of China Welfare Institute, Shanghai 200030, China.
authors.forename: Zhunan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Cervical Department, International Peace Maternity & Child Health Hospital of China Welfare Institute, Shanghai 200030, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Cervical Department, International Peace Maternity & Child Health Hospital of China Welfare Institute, Shanghai 200030, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yu
authors.affiliation: Cervical Department, International Peace Maternity & Child Health Hospital of China Welfare Institute, Shanghai 200030, China.
authors.forename: Ruosi
authors.initials: R
authors.lastname: Ji
mesh_terms: D003127:Colposcopy; D004598:Electrosurgery; D005260:Female; D006801:Humans; D030361:Papillomavirus Infections; D011247:Pregnancy; D012189:Retrospective Studies; D065310:Squamous Intraepithelial Lesions of the Cervix; D002583:Uterine Cervical Neoplasms