title: Loss of NFIX Transcription Factor Biases Postnatal Neural Stem/Progenitor Cells Toward Oligodendrogenesis.
abstract: Murine postnatal neural stem cells (NSCs) give rise to neurons, astrocytes, or oligodendrocytes (OLs); however, our knowledge of the genes that control this lineage specification is incomplete. In this study, we show that nuclear factor I X (NFIX), a transcription factor known to regulate NSC quiescence, also suppresses oligodendrogenesis (ODG) from NSCs. Immunostaining reveals little or no expression of NFIX in OL lineage cells both in vivo and in vitro. Loss of NFIX from subventricular zone (SVZ) NSCs results in enhanced ODG both in vivo and in vitro, while forced expression of NFIX blocks NSC differentiation into OLs in vitro. RNA-seq analysis shows that genes previously shown to be differentially expressed in OL progenitors are significantly enriched in RNA from Nfix(-/-) versus wild-type NSCs. These data indicate that NFIX influences the lineage specification of postnatal SVZ NSCs, specifically suppressing ODG.
authors.affiliation: 1 Department of Biochemistry, Genomics and Bioinformatics Program, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo , Buffalo, New York.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: 1 Department of Biochemistry, Genomics and Bioinformatics Program, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo , Buffalo, New York.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Osinski
authors.affiliation: 2 Centre for Organismal Studies Heidelberg, University of Heidelberg , Heidelberg, Germany .
authors.forename: Juan L
authors.initials: JL
authors.lastname: Mateo
authors.affiliation: 3 Division of Molecular Neurobiology, MRC , London, United Kingdom .
authors.forename: Ben
authors.initials: B
authors.lastname: Martynoga
authors.affiliation: 4 Department of Genetics, Genomics and Bioinformatics Program, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo , Buffalo, New York.|5 Department of Pharmacology and Toxicology, State University of New York at Buffalo , Buffalo, New York.
authors.forename: Fraser J
authors.initials: FJ
authors.lastname: Sim
authors.affiliation: 1 Department of Biochemistry, Genomics and Bioinformatics Program, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo , Buffalo, New York.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Campbell
authors.affiliation: 3 Division of Molecular Neurobiology, MRC , London, United Kingdom .
authors.forename: Francois
authors.initials: F
authors.lastname: Guillemot
authors.affiliation: 6 School of Biomedical Sciences, Queensland Brain Institute, The University of Queensland , Brisbane, Australia .
authors.forename: Michael
authors.initials: M
authors.lastname: Piper
authors.affiliation: 1 Department of Biochemistry, Genomics and Bioinformatics Program, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo , Buffalo, New York.|4 Department of Genetics, Genomics and Bioinformatics Program, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo , Buffalo, New York.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Gronostajski
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology; D019070:Cell Lineage; D002478:Cells, Cultured; D020547:Lateral Ventricles / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051839:NFI Transcription Factors / Q000235:genetics*; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology*; D055757:SOXE Transcription Factors / Q000378:metabolism
chemical_list: D051839:NFI Transcription Factors; C497650:Nfix protein, mouse; D055757:SOXE Transcription Factors; C527924:Sox10 protein, mouse