title: Biodiversity and degradation potential of oil-degrading bacteria isolated from deep-sea sediments of South Mid-Atlantic Ridge.
abstract: The indigenous oil-degrading bacterial consortia MARA and MARB were enriched from the deep-sea sediments of South Mid-Atlantic Ridge (MAR) with crude oil as sole carbon and energy sources. Biodiversity and community analyses showed that members of α-Proteobacteria were the key players in consortium MARA, whereas those of γ-Proteobacteria were the key players in consortium MARB, which were studied by MiSeq sequencing method. Gravimetric method estimated the oil degradation rates of MARA and MARB to be 63.4% and 85.8%, respectively, after 20d. Eleven cultivable oil degraders with different morphologies were isolated. These strains were identified as Alcanivorax, Bacillus, Dietzia, Erythrobacter, Marinobacter, Nitratireductor, and Oceanicola based on 16S rRNA gene sequences. Three strains belonging to Dietzia exhibited the highest oil degradation capability. Results indicated that the intrinsic biodegradation capacity of oil contaminants by indigenous microbial communities exists in South MAR sediments.
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China.
authors.forename: Xiangxing
authors.initials: X
authors.lastname: Gao
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China; College of Marine life, Ocean University of China, Qingdao, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China.
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Peihua
authors.initials: P
authors.lastname: Yang
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Sun
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China. Electronic address: Zhengli@fio.org.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
mesh_terms: D001267:Atlantic Ocean; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019015:Geologic Sediments / Q000382:microbiology; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D010578:Petroleum; D012336:RNA, Ribosomal, 16S
keywords: Crude oil biodegradation; Metagenome; Microbial consortium; Mid-Atlantic Ridge; Sediments