title: Preparation of 4-arm star PELA and its encapsulation of rotavirus for drug delivery.
abstract: A relatively high molecular weight of 4-arm star PELA was obtained by ring-opening polymerization of l-lactic acid O-carboxyanhydride with 4-arm-PEG in the presence of DMAP as an initiator. The results via(1)H NMR and (13)C NMR show that the end of the star PELA chain is a hydroxyl group and the central core is a PEG group. Rotavirus (strain SA11) was incorporated into 4-arm star PELA microspheres formulated by the water in oil in water emulsification solvent extraction method. The microspheres produced were spherical, and the mean diameter was 1.34 μm with a narrow size distribution. The drug release profile displayed a low burst release effect of 1.8% on the first day and a sustained release of antigen over 100 days. After the immunization of mice, the microsphere-entrapped RV elicited improved and long-lasting IgA and IgG antibody response in serum detected by ELISA in comparison to the free RV antigen. This study shows that 4-arm-PEG is an effective initiator for the ring-opening polymerization of Lac-OCA by DMAP as an initiator and that the resulting polymer is useful as a delivery system for the rotavirus vaccine.
authors.affiliation: Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qingcong
authors.affiliation: Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Xiaoxia
authors.affiliation: Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hongli
authors.affiliation: Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, China. Electronic address: yml@188.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Minglong
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003692:Delayed-Action Preparations / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D007773:Lactates / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012401:Rotavirus / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000941:Antigens; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D007773:Lactates; D009821:Oils; D011108:Polymers; D012997:Solvents; D014867:Water; C058523:polyethylene oxide-polylactic acid block copolymer; D019344:Lactic Acid; D011092:Polyethylene Glycols
keywords: 4-arm star PELA; Immune response; Microspheres; Preparation; Rotavirus