title: A single base substitution in BADH/AMADH is responsible for fragrance in cucumber (Cucumis sativus L.), and development of SNAP markers for the fragrance.
abstract: Sequence analysis revealed that an SNP (A1855G) in CsBADH of cucumber accession PK2011T202 causes amino acid change in a highly conserved motif, Y163C. Gene mapping showed association between the SNP and the fragrance. Pandan-like fragrance is a value-added trait in several food crops such as rice, vegetable soybean and sorghum. The fragrance is caused by the volatile chemical 2-acetyl-1-pyrroline (2AP). Mutation(s) in betaine aldehyde dehydrogenase 2 (BADH2; also known as aminoaldehyde dehydrogenase 2) gene causes defective BADH2 and results in biosynthesis of 2AP. Recently, cucumber cultivars possessing pandan-like fragrance were discovered in Thailand. In this study, we report an association between CsBADH and the fragrance in cucumber accession "PK2011T202". Gene expression analysis of CsBADH in leaves of PK2011T202 and "301176" (non-fragrant) at various growth stages revealed that CsBADH was expressed in both accessions. Sequence comparison of CsBADH showed that PK2011T202 possesses a single base substitution (A1855G) in exon 5 which causes an amino acid change in a highly conserved motif of BADH, Y163C. Single nucleotide-amplified polymorphism markers were developed to detect the SNP polymorphism between the wild-type and fragrance alleles. Since CsBADH is located on chromosome 1, quantitative trait locus (QTL) mapping was conducted for this chromosome using an F2 and a backcross populations developed from the cross between PK2011T202 and 301176. QTL analysis in both populations showed that the major QTL for fragrance, qFgr, was co-localized with the CsBADH. We concluded that the defect function of CsBADH is responsible for fragrance in cucumber PK2011T202.
authors.affiliation: Program in Plant Breeding, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand.
authors.forename: Chutintorn
authors.initials: C
authors.lastname: Yundaeng
authors.forename: Prakit
authors.initials: P
authors.lastname: Somta
authors.forename: Sithichoke
authors.initials: S
authors.lastname: Tangphatsornruang
authors.forename: Sompong
authors.initials: S
authors.lastname: Chankaew
authors.forename: Peerasak
authors.initials: P
authors.lastname: Srinives
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D050728:Betaine-Aldehyde Dehydrogenase / Q000235:genetics*; D002874:Chromosome Mapping; D018553:Cucumis sativus / Q000201:enzymology / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009812:Odorants; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011758:Pyrroles / Q000737:chemistry; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins; D011758:Pyrroles; D050728:Betaine-Aldehyde Dehydrogenase; C426303:2-acetyl-1-pyrroline