title: Characterization of Lactobacillus fermentum PL9988 Isolated from Healthy Elderly Korean in a Longevity Village.
abstract: In this work, we wanted to develop a probiotic from famous longevity villages in Korea. We visited eight longevity villages in Korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. Isolated colonies that appeared on MRS agar containing bromophenol blue were identified by means of 16S rRNA sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 species). Lactobacillus fermentum was the most frequently found species. Eight isolates were selected on the basis of their ability to inhibit the growth of six intestinal pathogens (Escherichia coli O157:H7, Salmonella enterica subsp. enterica Typhimurium, Salmonella enterica subsp. enterica Enteritidis, Enterococcus faecalis, Staphylococcus aureus, and Listeria monocytogenes) and their susceptibility to 15 antimicrobial agents. Among these eight isolates, four Lactobacillus fermentum isolates were found not to produce any harmful enzymes or metabolites. Among them, Lactobacillus fermentum isolate no. 24 showed the strongest binding to intestinal epithelial cells, the highest immune-enhancing activity, anti-inflammation activity, and anti-oxidation activity as well as the highest survival rates in the presence of artificial gastric juice and bile solution. This isolate, designated Lactobacillus fermentum PL9988, has all the characteristics for a good probiotic.
authors.affiliation: R&D Center, Namyang Dairy Products Co., Sejong 339-914, Republic of Korea.
authors.forename: Jong-Su
authors.initials: JS
authors.lastname: Park
authors.affiliation: Culture Collection of Antimicrobial Resistant Microbes (CCARM), Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Eunju
authors.initials: E
authors.lastname: Shin
authors.affiliation: Culture Collection of Antimicrobial Resistant Microbes (CCARM), Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Hong
authors.affiliation: R&D Center, Namyang Dairy Products Co., Sejong 339-914, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Shin
authors.affiliation: R&D Center, Namyang Dairy Products Co., Sejong 339-914, Republic of Korea.
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Hyanglim Co., Chunan 331-280, Republic of Korea.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Ahn
authors.affiliation: Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.
authors.forename: Kyungsoo
authors.initials: K
authors.lastname: Paek
authors.affiliation: Culture Collection of Antimicrobial Resistant Microbes (CCARM), Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000368:Aged; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000898:Antibiosis; D044466:Asian People; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000032:analysis; D002460:Cell Line; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D013293:Enterococcus faecalis; D004798:Enzymes / Q000032:analysis; D004847:Epithelial Cells / Q000382:microbiology; D019453:Escherichia coli O157; D005243:Feces / Q000382:microbiology*; D006801:Humans; D052197:Limosilactobacillus fermentum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008089:Listeria monocytogenes; D008136:Longevity; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D016877:Oxidants / Q000633:toxicity; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D016877:Oxidants; D012336:RNA, Ribosomal, 16S
keywords: Lactobacillus fermentum; lactic acid-producing bacteria; longevity; probiotic