title: Chimeric Protein Template-Induced Shape Control of Bone Mineral Nanoparticles and Its Impact on Mesenchymal Stem Cell Fate.
abstract: Protein-mediated molecular self-assembly has become a powerful strategy to fabricate biomimetic biomaterials with controlled shapes. Here we designed a novel chimeric molecular template made of two proteins, silk fibroin (SF) and albumin (ALB), which serve as a promoter and an inhibitor for hydroxyapatite (HA) formation, respectively, to synthesize HA nanoparticles with controlled shapes. HA nanospheres were produced by the chimeric ALB-SF template, whereas HA nanorods were generated by the SF template alone. The success in controlling the shape of HA nanoparticles allowed us to further study the effect of the shape of HA nanoparticles on the fate of rat mesenchymal stem cells (MSCs). We found that the nanoparticle shape had a crucial impact on the cellular uptake and HA nanospheres were internalized in MSCs at a faster rate. Both HA nanospheres and nanorods showed no significant influence on cell proliferation and migration. However, HA nanospheres significantly promoted the osteoblastic differentiation of MSCs in comparison to HA nanorods. Our work suggests that a chimeric combination of promoter and inhibitor proteins is a promising approach to tuning the shape of nanoparticles. It also sheds new light into the role of the shape of the HA nanoparticles in directing stem cell fate.
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.|Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
authors.forename: Gaojie
authors.initials: G
authors.lastname: Yang
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
authors.forename: Yinying
authors.initials: Y
authors.lastname: Du
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
authors.forename: Haoming
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Mao
authors.affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
authors.forename: Shengmin
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D002113:Calcification, Physiologic; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D005349:Fibroins / Q000235:genetics* / Q000378:metabolism; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D053758:Nanoparticles / Q000737:chemistry; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D023822:Tissue Engineering
chemical_list: D000418:Albumins; D001672:Biocompatible Materials; D011994:Recombinant Proteins; D005349:Fibroins; D017886:Durapatite