title: Mass spectrometry imaging of therapeutics from animal models to three-dimensional cell cultures.
abstract: Mass spectrometry imaging (MSI) is a powerful label-free technique for the investigation of the spatial distribution of molecules at complex surfaces and has been widely used in the pharmaceutical sciences to understand the distribution of different drugs and their metabolites in various biological samples, ranging from cell-based models to tissues. Here, we review the current applications of MSI for drug studies in animal models, followed by a discussion of the novel advances of MSI in three-dimensional (3D) cell cultures for accurate, efficient, and high-throughput analyses to evaluate therapeutics.
authors.affiliation: Department of Chemistry and Biochemistry, Harper Cancer Research Institute, University of Notre Dame , 251 Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biochemistry, Harper Cancer Research Institute, University of Notre Dame , 251 Nieuwland Science Hall, Notre Dame, Indiana 46556, United States.
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Hummon
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D057166:High-Throughput Screening Assays; D013058:Mass Spectrometry; D023421:Models, Animal; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism
chemical_list: D004364:Pharmaceutical Preparations