title: Platelet-bound cyclophilin A in patients with stable coronary artery disease and acute myocardial infarction.
abstract: OBJECTIVE
  Recently, we reported that extracellular cyclophilin A (CyPA) is an important agonist for platelets. Whereas soluble CyPA-levels have been associated with cardiovascular risk factors, cell-bound CyPA has not been investigated yet. In this study, we analyzed for the first time platelet-bound CyPA in patients with symptomatic coronary artery disease (CAD).
  
  
  RESULTS
  blood was obtained from 388 consecutive patients: 204 with stable CAD and 184 with acute coronary syndrome (76 with unstable angina, 78 with non ST-elevation myocardial infarction (NSTEMI), and 30 with STEMI). In vitro stimulation of platelets with classical agonists revealed an enhanced expression of CyPA on the platelet surface. In patients with stable CAD, platelet-bound CyPA correlated excellently with platelet activity measured by P-selectin exposure in flow cytometry. The analysis of classical risk factors for atherosclerosis revealed that patients with hypertension and hypercholesterolemia had significantly enhanced platelet-bound CyPA, whereas diabetes and smoking were not associated with enhanced CyPA-binding to the platelet surface. In multivariate analysis, hypercholesterolemia was the only significant predictor of enhanced platelet-bound CyPA. Interestingly, in patients with acute myocardial infarction (AMI) platelet-bound CyPA was significantly decreased compared with patients with stable CAD.
  
  
  CONCLUSIONS
  Enhanced platelet-bound CyPA is associated with hypertension and hypercholesterolemia in stable CAD patients. In patients with AMI platelet-bound CyPA is significantly decreased.
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Seizer
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Fuchs
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Saskia N I V
authors.initials: SN
authors.lastname: Ungern-Sternberg
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: David
authors.initials: D
authors.lastname: Heinzmann
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Langer
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Meinrad
authors.initials: M
authors.lastname: Gawaz
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Andreas E
authors.initials: AE
authors.lastname: May
authors.affiliation: a Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität Tübingen , Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Geisler
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000789:Angina, Unstable / Q000097:blood* / Q000150:complications / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D003324:Coronary Artery Disease / Q000097:blood* / Q000150:complications / Q000473:pathology; D021984:Cyclophilin A / Q000097:blood* / Q000235:genetics; D003920:Diabetes Mellitus / Q000503:physiopathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood* / Q000150:complications / Q000473:pathology; D006973:Hypertension / Q000097:blood* / Q000150:complications / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood / Q000235:genetics; D015539:Platelet Activation; D011485:Protein Binding; D012307:Risk Factors; D012907:Smoking / Q000503:physiopathology
chemical_list: D019007:P-Selectin; D021984:Cyclophilin A
keywords: Acute coronary syndrome; Cyclophilin A; platelets; thrombosis