title: Multimeric Near IR-MR Contrast Agent for Multimodal In Vivo Imaging.
abstract: Multiple imaging modalities are often required for in vivo imaging applications that require both high probe sensitivity and excellent spatial and temporal resolution. In particular, MR and optical imaging are an attractive combination that can be used to determine both molecular and anatomical information. Herein, we describe the synthesis and in vivo testing of two multimeric NIR-MR contrast agents that contain three Gd(III) chelates and an IR-783 dye moiety. One agent contains a PEG linker and the other a short alkyl linker. These agents label cells with extraordinary efficacy and can be detected in vivo using both imaging modalities. Biodistribution of the PEGylated agent shows observable fluorescence in xenograft MCF7 tumors and renal clearance by MR imaging.
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
authors.forename: Victoria S R
authors.initials: VS
authors.lastname: Harrison
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
authors.forename: Christiane E
authors.initials: CE
authors.lastname: Carney
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
authors.forename: Keith W
authors.initials: KW
authors.lastname: MacRenaris
authors.affiliation: ‡Center for Advanced Molecular Imaging, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Waters
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.|‡Center for Advanced Molecular Imaging, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Meade
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D005260:Female; D005682:Gadolinium / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D007259:Infrared Rays; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging; D008819:Mice, Nude; D064847:Multimodal Imaging; D009369:Neoplasms / Q000175:diagnosis; D061848:Optical Imaging; D014018:Tissue Distribution
chemical_list: D002614:Chelating Agents; D004396:Coloring Agents; D003287:Contrast Media; D005682:Gadolinium