title: Identification of peanut seed prolamins with an antifungal role by 2D-GE and drought treatment.
abstract: This work revealed peanut seed prolamins likely displaying a defensive role besides the known nitrogen storage. Drought stress and proteomic approaches were used in varieties of peanuts to explore the prolamin member in association with a test against Aspergillus flavus spore germination. The stress effect was showed by aerial biomass, leaf content of malondialdehyde, and seed contamination by A. flavus. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles were not informative for the antifungal polypeptides. From two-dimensional gel electrophoresis, the suspected polypeptides were those with pI 5.45-5.75 and sizes of 22.0-30.5 kDa specifically in Spanish-type peanuts. Regarding to the drought effect in most of these peanuts, the spot peak volume analysis deduced three novel prolamin-related antifungal polypeptides at pI 5.75-5.8 with 30.5, 27.5-28.5, and 22.0-22.5 kDa, which was confirmed after isoelectric purification at pH 5.60. The data could not yet conclude their correlation with resistance to drought and to seed infection by A. flavus.
authors.affiliation: a Faculty of Science, Department of Biochemistry , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Waraluk
authors.initials: W
authors.lastname: Senakoon
authors.affiliation: a Faculty of Science, Department of Biochemistry , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Suporn
authors.initials: S
authors.lastname: Nuchadomrong
authors.affiliation: b Department of Food Science, College of Life Sciences , National Chiayi University , Chiayi , Taiwan, ROC.
authors.forename: Robin Y-Y
authors.initials: RY
authors.lastname: Chiou
authors.affiliation: a Faculty of Science, Department of Biochemistry , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Gulsiri
authors.initials: G
authors.lastname: Senawong
authors.affiliation: c Faculty of Agriculture, Department of Plant Science and Agricultural Resources , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Sanun
authors.initials: S
authors.lastname: Jogloy
authors.affiliation: c Faculty of Agriculture, Department of Plant Science and Agricultural Resources , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Patcharin
authors.initials: P
authors.lastname: Songsri
authors.affiliation: c Faculty of Agriculture, Department of Plant Science and Agricultural Resources , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Aran
authors.initials: A
authors.lastname: Patanothai
mesh_terms: D000935:Antifungal Agents; D010367:Arachis / Q000737:chemistry / Q000235:genetics*; D001231:Aspergillus flavus / Q000378:metabolism / Q000472:pathogenicity; D055864:Droughts; D004591:Electrophoresis, Polyacrylamide Gel; D009584:Nitrogen / Q000378:metabolism*; D010455:Peptides; D055315:Prolamins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012639:Seeds / Q000737:chemistry; D013312:Stress, Physiological
chemical_list: D000935:Antifungal Agents; D010455:Peptides; D055315:Prolamins; D009584:Nitrogen
keywords: 2D-GE maps; Aspergillus flavus; antifungus; peanut seed prolamins