title: Protection of Lactobacillus acidophilus NRRL-B 4495 under in vitro gastrointestinal conditions with whey protein/pullulan microcapsules.
abstract: In this research, whey protein/pullulan (WP/pullulan) microcapsules were developed in order to assess its protective effect on the viability of Lactobacillus acidophilus NRRL-B 4495 under in vitro gastrointestinal conditions. Results demonstrated that WP/pullulan microencapsulated cells exhibited significantly (p ≤ 0.05) higher resistance to simulated gastric acid and bile salt. Pullulan incorporation into protein wall matrix resulted in improved survival as compared to free cells after 3 h incubation in simulated gastric solution. Moreover WP/pullulan microcapsules were found to release over 70% of encapsulated L. acidophilus NRRL-B 4495 cells within 1 h. The effect of encapsulation during refrigerated storage was also studied. Free bacteria exhibited 3.96 log reduction while, WP/pullulan encapsulated bacteria showed 1.64 log reduction after 4 weeks of storage.
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Gülbahçe Köyü, Urla, Izmir, Turkey. Electronic address: burcucabuk@iyte.edu.tr.
authors.forename: Burcu
authors.initials: B
authors.lastname: Çabuk
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Gülbahçe Köyü, Urla, Izmir, Turkey. Electronic address: sebnemharsa@iyte.edu.tr.
authors.forename: Şebnem
authors.initials: Ş
authors.lastname: Tellioğlu Harsa
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism*; D002214:Capsules; D003080:Cold Temperature; D004339:Drug Compounding; D004355:Drug Stability; D004356:Drug Storage / Q000379:methods; D005744:Gastric Acid / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D019936:Probiotics / Q000008:administration & dosage* / Q000378:metabolism*; D013997:Time Factors; D000067816:Whey Proteins / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D002214:Capsules; D005936:Glucans; D000067816:Whey Proteins; C009109:pullulan
keywords: Encapsulation; Lactobacillus acidophilus; Probiotic; Pullulan; Whey protein