title: Strategies for Engineering Protein N-Glycosylation Pathways in Mammalian Cells.
abstract: Complexity and heterogeneity of oligosaccharides present a considerable challenge to the biopharmaceutical industry to manufacture biotherapeutics with reproducible and consistent glycoform profiles. Mammalian cells, especially Chinese hamster ovary cells, are the most widely used platform for the production of biotherapeutics. The glycans produced are predominantly of the complex type, with some differences between human and nonhuman mammalian glycosylation existing. This review briefly summarizes metabolic glyco-engineering strategies used in mammalian cells in order to alter the glycosylation patterns attached to proteins applied for diverse biotechnology applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Mark
authors.initials: M
authors.lastname: Stuczynski
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Betenbaugh
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D016466:CHO Cells; D003412:Cricetulus; D005818:Genetic Engineering / Q000379:methods; D006031:Glycosylation; D006801:Humans; D008322:Mammals / Q000235:genetics*; D011134:Polysaccharides / Q000235:genetics; D011506:Proteins / Q000235:genetics*
chemical_list: D011134:Polysaccharides; D011506:Proteins