title: [Expression and relationship of pendrin and MUC5AC in patients with chronic rhinosinusitis].
abstract: OBJECTIVE
  To explore the expression of pendrin in sinonasal mucosal tissue of patients with chronic rhinosinusitis (CRS) and examine its relationship with mucin5AC (MUC5AC).
  
  
  METHODS
  Thirty mucosal tissue (located on anterior wall of maxillary sinus ostium and close to uncinate process) of patients with CRS with or without nasal polyps and 10 normal sinonasal mucosal tissue located at the same sites as CRS were collected. Histological changes of sinonasal mucosa were examined by hematoxylin and eosin (HE) staining. The expression of total mucins was evaluated by periodic acid Schiff staining (PAS). And the expressions of pendrin and MUC5AC in sinonasal mucosa were determined by immunohistochemistry (IHC), quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot.
  
  
  RESULTS
  The tissue samples demonstrated mucosal thickening, goblet cell hyperplasia and metaplasia, epithelial cilia partial deletion, glandular hyperplasia and a dense infiltration of inflammatory cells in CRS with or without nasal polyps. IHC revealed that positive pendrin was expressed dominantly at apical membrane of epithelial cells and sparsely in submucosal glandular cells, there was a positive expression rate of CRS with nasal polyps and CRS without nasal polyps; MUC5AC was expressed abundantly in goblet cells and pseudostratified ciliated columnar epithelium cells and weakly in submucosal glandular cells in CRS. There were significant increases in positive expression rate of total mucins production, pendrin and MUC5AC in patients of CRS with nasal polyps or without nasal polyps than those in control (86.7% and 80.0% vs 20.0%, 73.3% and 70.0% vs 10.0%, 83.3% and 76.7% vs 20.0%, all P<0.05); the relative expression levels of mRNA and protein expression of pendrin were significantly greater in patients of CRS with or without nasal polyps than those in controls (1.685±0.111 and 1.537±0.262 vs 1.012±0.167, 2.066±0.188 and 1.996±0.153 vs 1.000±0.169, all P<0.05). The relative expression levels of mRNA expression of MUC5AC were significantly greater in patients of CRS with or without nasal polyps than in controls (1.861±0.718 and 1.562±0.428 vs 1.004±0.094, P<0.05). And the expression level of pendrin mRNA was positively correlated with that of MUC5AC mRNA in patients with CRS (r=0.670, P=0.006 and r=0.713, P=0.003).
  
  
  CONCLUSIONS
  An up-regulation of pendrin is correlated with an over-expression of MUC5AC in patients with CRS. And pendrin is an important cause of mucus hypersecretion in CRS.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Feng
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, China; Email: lqfri582@sina.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
mesh_terms: D002908:Chronic Disease; D004847:Epithelial Cells; D006801:Humans; D007150:Immunohistochemistry; D055271:Mucin 5AC; D009093:Mucus; D009297:Nasal Mucosa; D009298:Nasal Polyps; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D012220:Rhinitis; D012852:Sinusitis; D015854:Up-Regulation
chemical_list: D055271:Mucin 5AC; D012333:RNA, Messenger