title: Neuregulin-1β Regulates the migration of Different Neurochemical Phenotypic Neurons from Organotypically Cultured Dorsal Root Ganglion Explants.
abstract: Neuregulin-1β (NRG-1β) has multiple roles in the development and function in the nervous system and exhibits potent neuroprotective properties. In the present study, organotypically cultured dorsal root ganglion (DRG) explants were used to evaluate the effects of NRG-1β on migration of two major phenotypic classes of DRG neurons. The signaling pathways involved in these effects were also determined. Organotypically cultured DRG explants were exposed to NRG-1β (20 nmol/L), the phosphatidylinositol 3-kinase inhibitor LY294002 (10 μmol/L) plus NRG-1β (20 nmol/L), the extracellular signal-regulated protein kinase (ERK1/2) inhibitor PD98059 (10 μmol/L) plus NRG-1β (20 nmol/L), and LY294002 (10 μmol/L) plus PD98059 (10 μmol/L) plus NRG-1β (20 nmol/L), respectively, for 3 days. The DRG explants were continuously exposed to culture media as a control. After that, all above cultures were processed for detecting the mRNA levels of calcitonin gene-related peptide (CGRP) and neurofilament-200 (NF-200) by real-time PCR analysis. CGRP and NF-200 expression in situ was determined by fluorescent labeling technique. The results showed that NRG-1β elevated the mRNA and protein levels of CGRP and NF-200. NRG-1β also increased the number and the percentage of CGRP-immunoreactive (IR) migrating neurons and NF-200-IR migrating neurons. Inhibitors (LY294002, PD98059) either alone or in combination blocked the effects of NRG-1β. The contribution of NRG-1β on modulating distinct neurochemical phenotypic plasticity of DRG neurons suggested that NRG-1β signaling system might play an important role on the biological effects of primary sensory neurons.
authors.affiliation: Department of Cardiosurgery, Shandong University Qilu Hospital, 107 West Wenhua Xi Road, Jinan, 250012, Shandong Province, China. yunfengli630@live.com.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Binzhou Medical College, Binzhou, 256603, China. liuguixiang57@163.com.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Shandong University Qilu Hospital, Jinan, 250012, China. lihao462929@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Cardiosurgery, Shandong University Qilu Hospital, 107 West Wenhua Xi Road, Jinan, 250012, Shandong Province, China. yanwenbi126@yahoo.com.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Bi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology*; D020890:Neuregulin-1 / Q000494:pharmacology*; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009924:Organ Culture Techniques / Q000379:methods*; D010641:Phenotype; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar
chemical_list: D020890:Neuregulin-1; D016900:Neurofilament Proteins; D012333:RNA, Messenger; C051774:neurofilament protein H; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D015740:Calcitonin Gene-Related Peptide
keywords: Calcitonin gene-related peptide; Dorsal root ganglion; Neuregulin-1β; Neurofilament 200; Neuron; Phenotype