title: [Effects of conditioned media for rat bone marrow-derived mesenchymal stem cells on palmitic acid-induced insulin resistance in HepG2 cells].
abstract: OBJECTIVE
  To study the effect of conditioned media for rat bone marrow mesenchymal stem cells (BMSCs-CM) on palmitic acid (PA)-induced insulin resistance (IR) in HepG2 cells and its underlying molecular mechanisms.
  
  
  METHODS
  HepG2 cells were treated with or without BMSCs-CM and L-DMEM in the presence or absence of PA.Glucose utilization in HepG2 cells were detected with PAS, glucose and glycogen measurements. Western blotting was used to assess the expression of phospho-insulin receptor substrate (p-IRS), phosphatidylinositol 3-kinase (PI3K) and p-AKT.
  
  
  RESULTS
  (1) Incubation of HepG2 cells with 0.25 mmol/L PA for 24 hours significantly increased the glucose concentration and decreased the glycogen content (P<0.05) in the media. (2) Treatment with BMSCs-CM significantly ameliorated the glucose and glycogen alteration in cells pretreated with PA (P<0.05), however, no obvious effect of BMSCs-CM on the cell glucose and glycogen production. (3) BMSCs-CM treatment also increased protein expression of p-IRS, PI3K and p-AKT in PA incubated HapG2 cells (P<0.05). The effect of BMSCs-CM on PI3K and p-AKT expression could be mimicked upon addition of 740Y-P, a PI3K agonist, but abolished by LY294002, a PI3K specific inhibitor.
  
  
  CONCLUSIONS
  BMSCs-CM could improve the insulin sensitivity in HepG2 cells pretreated with PA through upregulation of insulin signaling component expression.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Sun
authors.forename: Haojie
authors.initials: H
authors.lastname: Hao
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China; Email: muyiming@301hospital.com.cn.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Mu
mesh_terms: D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology*; D002867:Chromones; D017077:Culture Media, Conditioned; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells / Q000187:drug effects*; D006801:Humans; D007328:Insulin; D007333:Insulin Resistance; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D009025:Morpholines; D019308:Palmitic Acid; D019869:Phosphatidylinositol 3-Kinases; D051381:Rats; D011972:Receptor, Insulin; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D002867:Chromones; D017077:Culture Media, Conditioned; D007328:Insulin; D009025:Morpholines; D019308:Palmitic Acid; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D011972:Receptor, Insulin; D005947:Glucose