title: Long term consequences on spatial learning-memory of low-calorie diet during adolescence in female rats; hippocampal and prefrontal cortex BDNF level, expression of NeuN and cell proliferation in dentate gyrus.
abstract: Calorie restriction (CR) is argued to positively affect general health, longevity and normally occurring age-related reduction of cognition. Obesity during adolescence may adversely affect cognition in adulthood but, to date effects of CR have not been investigated. We hypothesized that feeding with as low as 15% low-calorie diet (LCD) during adolescence would increase hippocampal and prefrontal BDNF (Brain-derived neurotrophic factor) levels, proliferative cells and neuron numbers in dentate gyrus (DG), thus positively affecting spatial memory in adulthood. Spatial learning-memory function was improved in adult female Sprague-Dawley rats fed with LCD during adolescence. PCNA (Proliferating cell nuclear antigen-cell proliferation marker) expressing cells and NeuN (Neuronal nuclear antigen-neuron marker) expressing cells in hippocampus DG which are critically involved in memory were increased. Hippocampus and prefrontal cortex BDNF levels were increased while serum glucose levels and level of lipid peroxidation indicator malondialdehyde in serum and hippocampus were reduced. Our unique results suggest that improved cognition in adult rats with LCD feeding during adolescence may result from the increase of neurogenesis and BDNF. These findings reveal the importance of nutrition in adolescence for cognitive function in adulthood. Our results may be useful for further studies aiming to treat age-related cognitive impairments.
authors.affiliation: Department of Physiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Zülal
authors.initials: Z
authors.lastname: Kaptan
authors.affiliation: Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: Akgün-Dar
authors.affiliation: Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Kapucu
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Marmara University, Istanbul, Turkey.
authors.forename: Huri
authors.initials: H
authors.lastname: Dedeakayoğulları
authors.affiliation: Department of Biochemistry, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
authors.forename: Şule
authors.initials: Ş
authors.lastname: Batu
authors.affiliation: Department of Physiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. Electronic address: gulayuzum@gmail.com.
authors.forename: Gülay
authors.initials: G
authors.lastname: Üzüm
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D031204:Caloric Restriction; D049109:Cell Proliferation / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D015227:Lipid Peroxidation; D018782:Maze Learning / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D065853:Spatial Learning / Q000502:physiology*; D065852:Spatial Memory / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D019208:Brain-Derived Neurotrophic Factor; D018809:Proliferating Cell Nuclear Antigen; D010751:Phosphopyruvate Hydratase
keywords: Adolescent; Calorie restriction; Hippocampus; Learning; Memory; Neurogenesis