title: Morphology and Molecular Phylogeny of Two Freshwater Peritrich Ciliates, Epistylis chlorelligerum Shen 1980 and Epistylis chrysemydis Bishop and Jahn 1941 (Ciliophora, Peritrichia).
abstract: Two populations of Epistylis chlorelligerum Shen 1980, a colonial limnetic peritrich ciliate, were collected from different locations in China: E. chlorelligerum 1 from West Lake, Hangzhou; E. chlorelligerum 2 from East Lake, Wuhan. The morphology, infraciliature and SSU rRNA gene sequence of the two populations were investigated based on living and protargol-stained specimens. Although both populations are consistent with previous descriptions of protargol-stained specimens of this species, some differences in the morphology in vivo were observed. The two populations had identical SSU rRNA gene sequences. A second species, Epistylis chrysemydis Bishop and Jahn 1941, was also collected from East Lake, Wuhan, and was investigated for its morphology, infraciliature and SSU rRNA gene sequence. Phylogenetic analyses based on SSU rRNA gene sequence data indicate that the two populations of E. chlorelligerum are nested within the Epistylididae clade near E. wenrichi and E. urceolata. Epistylis chrysemydis is sister to the group comprising E. chlorelligerum, E. wenrichi, and E. urceolata.
authors.affiliation: Hangzhou Key Laboratory for Animal Sciences and Technology, School of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, China.
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Hangzhou Key Laboratory for Animal Sciences and Technology, School of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, China.|Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China.
authors.forename: Xinlu
authors.initials: X
authors.lastname: Shi
authors.affiliation: Hangzhou Key Laboratory for Animal Sciences and Technology, School of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Liu
authors.affiliation: Hangzhou Key Laboratory for Animal Sciences and Technology, School of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Life Sciences, Natural History Museum, London, SW7 5BD, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Warren
mesh_terms: D002681:China; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017125:Genes, Protozoan; D020459:Genes, rRNA; D060106:Lakes; D016802:Oligohymenophorea / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Infraciliature; SSU rRNA