title: Establishment of three cell lines from Chinese giant salamander and their sensitivities to the wild-type and recombinant ranavirus.
abstract: Known as lethal pathogens, Ranaviruses have been identified in diseased fish, amphibians (including Chinese giant salamander Andrias davidianus, the world's largest amphibian) and reptiles, causing organ necrosis and systemic hemorrhage. Here, three Chinese giant salamander cell lines, thymus cell line (GSTC), spleen cell line (GSSC) and kidney cell line (GSKC) were initially established. Their sensitivities to ranaviruses, wild-type Andrias davidianus ranavirus (ADRV) and recombinant Rana grylio virus carrying EGFP gene (rRGV-EGFP) were tested. Temporal transcription pattern of ranavirus major capsid protein (MCP), fluorescence and electron microscopy observations showed that both the wild-type and recombinant ranavirus could replicate in the cell lines.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Wuhan, 430072, China. yuanjiangdi@ihb.ac.cn.
authors.forename: Jiang-Di
authors.initials: JD
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Wuhan, 430072, China. chenzy@ihb.ac.cn.
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Wuhan, 430072, China. huangxing@ihb.ac.cn.
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Wuhan, 430072, China. gaoxiaochan@ihb.ac.cn.
authors.forename: Xiao-Chan
authors.initials: XC
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Wuhan, 430072, China. zhangqy@ihb.ac.cn.
authors.forename: Qi-Ya
authors.initials: QY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line / Q000821:virology*; D004266:DNA Virus Infections / Q000662:veterinary* / Q000821:virology; D007668:Kidney / Q000821:virology; D017995:Ranavirus / Q000235:genetics / Q000502:physiology*; D016865:Reassortant Viruses / Q000235:genetics / Q000502:physiology; D013154:Spleen / Q000821:virology; D013950:Thymus Gland / Q000821:virology; D014562:Caudata