title: Identification and expression pattern of candidate olfactory genes in Chrysoperla sinica by antennal transcriptome analysis.
abstract: Chrysoperla sinica is one of the most prominent natural enemies of many agricultural pests. Host seeking in insects is strongly mediated by olfaction. Understanding the sophisticated olfactory system of insect antennae is crucial for studying the physiological bases of olfaction and could also help enhance the effectiveness of C. sinica in biological control. Obtaining olfactory genes is a research priority for investigating the olfactory system in this species. However, no olfaction sequence information is available for C. sinica. Consequently, we sequenced female- and male-antennae transcriptome of C. sinica. Many candidate chemosensory genes were identified, including 12 odorant-binding proteins (OBPs), 19 chemosensory proteins (CSPs), 37 odorant receptors (ORs), and 64 ionotropic receptors from C. sinica. The expression patterns of 12 OBPs, 19 CSPs and 37 ORs were determined by RT-PCR, and demonstrated antennae-dominantly expression of most OBP and OR genes. Our finding provided large scale genes for further investigation on the olfactory system of C. sinica at the molecular level.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China; Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China; Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Si-Bao
authors.initials: SB
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Lv
authors.affiliation: Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: sldong@njau.edu.cn.
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China. Electronic address: cuijinjie@126.com.
authors.forename: Jin-Jie
authors.initials: JJ
authors.lastname: Cui
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics; D007313:Insecta / Q000235:genetics*; D008297:Male
chemical_list: D019476:Insect Proteins
keywords: Chemosensory proteins; Chrysoperla sinica; Odorant receptors; Odorant-binding proteins; Transcriptomic analysis