title: Altered protein expression pattern in skin fibroblasts from parkin-mutant early-onset Parkinson's disease patients.
abstract: Parkinson's disease (PD) is the most common neurodegenerative movement disorder caused primarily by selective degeneration of the dopaminergic neurons in substantia nigra. In this work the proteomes extracted from primary fibroblasts of two unrelated, hereditary cases of PD patients, with different parkin mutations, were compared with the proteomes extracted from commercial adult normal human dermal fibroblasts (NHDF) and primary fibroblasts from the healthy mother of one of the two patients. The results show that the fibroblasts from the two different cases of parkin-mutant patients display analogous alterations in the expression level of proteins involved in different cellular functions, like cytoskeleton structure-dynamics, calcium homeostasis, oxidative stress response, protein and RNA processing.
authors.affiliation: Institute of Biomembranes and Bioenergetics, Italian National Research Council (CNR), Via G. Amendola 165/A, Bari, Italy. Electronic address: r.lippolis@ibbe.cnr.it.
authors.forename: Rosa
authors.initials: R
authors.lastname: Lippolis
authors.affiliation: Institute of Food Sciences, Italian National Research Council (CNR), Via Roma, 64, Avellino, Italy.
authors.forename: Rosa Anna
authors.initials: RA
authors.lastname: Siciliano
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Universitè de Montreal, 2900 Boulevard Edouard-Montpetit, Montreal QCH3T1J4, Canada.
authors.forename: Consiglia
authors.initials: C
authors.lastname: Pacelli
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Ferretta
authors.affiliation: Institute of Food Sciences, Italian National Research Council (CNR), Via Roma, 64, Avellino, Italy.
authors.forename: Maria Fiorella
authors.initials: MF
authors.lastname: Mazzeo
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Scacco
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Papa
authors.affiliation: CNR NANOTEC-Istituto di Nanotecnologia, Polo di Nanotecnologia c/o Campus Ecotekne, via Monteroni, 73100 Lecce, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gaballo
authors.affiliation: Neurology Unit, Bonomo Hospital, Andria, BA, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Dell'Aquila
authors.affiliation: Neurology Unit, Bonomo Hospital, Andria, BA, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: De Mari
authors.affiliation: Institute of Biomembranes and Bioenergetics, Italian National Research Council (CNR), Via G. Amendola 165/A, Bari, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy. Electronic address: tizianamaria.cocco@uniba.it.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cocco
keywords: Human skin fibroblasts; Mass spectrometry; Parkinson's disease; Proteomics; Two-dimensional gel electrophoresis