title: Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress.
abstract: Drought is one of the most widespread stresses leading to retardation of plant growth and development. We examined proteome changes of an important oil seed crop, canola (Brassica napus L.), under drought stress over a 14-day period. Using iTRAQ LC-MS/MS, we identified 1976 proteins expressed during drought stress. Among them, 417 proteins showed significant changes in abundance, and 136, 244, 286, and 213 proteins were differentially expressed in the third, seventh, 10th, and 14th day of stress, respectively. Functional analysis indicated that the number of proteins associated with metabolism, protein folding and degradation, and signaling decreased, while those related to energy (photosynthesis), protein synthesis, and stress and defense increased in response to drought stress. The seventh and 10th-day profiles were similar to each other but with more post-translational modifications (PTMs) at day 10. Interestingly, 181 proteins underwent PTMs; 49 of them were differentially changed in drought-stressed plants, and 33 were observed at the 10th day. Comparison of protein expression changes with those of gene transcription showed a positive correlation in B. napus, although different patterns between transcripts and proteins were observed at each time point. Under drought stress, most protein abundance changes may be attributed to gene transcription, and PTMs clearly contribute to protein diversity and functions.
authors.forename: Jin
authors.initials: J
authors.lastname: Koh
authors.affiliation: §Yangzhou University, Yangzhou, 225009 Jiangsu, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Yoo
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.affiliation: ⊥Palm Beach Central High School, Wellington, Florida 33411, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Dufresne
authors.forename: John E
authors.initials: JE
authors.lastname: Erickson
authors.affiliation: #Yantai Institute of Costal Zone Research, Chinese Academy of Sciences, Yantai, 264003 Shandong, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Shao
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Brassica napus; PTMs; drought stress; iTRAQ; proteomics; transcription