title: Ebselen reduces autophagic activation and cell death in the ipsilateral thalamus following focal cerebral infarction.
abstract: Previous studies have demonstrated that both oxidative stress and autophagy play important roles in secondary neuronal degeneration in the ipsilateral thalamus after distal middle cerebral artery occlusion (MCAO). This study aimed to investigate whether oxidative stress is associated with autophagy activation within the ipsilateral thalamus after distal MCAO. Sixty stroke-prone renovascular hypertensive rats were subjected to distal MCAO or sham operation, and were killed at 14 days after MCAO. Mn-SOD, LC3-II, Beclin-1 and p62 expression were evaluated by immunostaining and immunoblotting. Secondary damage in the thalamus was assessed with Nissl staining and immunostaining. The association of oxidative stress with autophagy activation was investigated by the antioxidant, ebselen. We found that treatment with ebselen at 24h after MCAO significantly reduced the expression of Mn-SOD in the ipsilateral thalamus at 14 days following focal cerebral infarction. In parallel, it prevented the elevation of LC3-II and Beclin-1, and the reduction of p62. Furthermore, ebselen attenuated the neuronal loss and gliosis in the ipsilateral thalamus. These results suggested that ebselen reduced oxidative stress, autophagy activation and secondary damage in the ipsilateral thalamus following MCAO. There are associations between oxidative stress, autophagy activation and secondary damage in the thalamus after MCAO.
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China; Department of Neurology, Department of Rehabilitation medicine, The Central Hospital of Loudi Affiliated to the University of South China, Loudi 417000, China.
authors.forename: Yiliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Xing
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, First Affiliated Hospital, Jinan University, No. 613 Huangpu Avenue West, Guangzhou 510630, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Guangzhou No. 8 People's Hospital, Guangzhou Medical University, Huaying Road 8, Guangzhou 510440, China.
authors.forename: Chuo
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Pei
authors.affiliation: Department of Neurology and Stroke Center, First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, China. Electronic address: zengjs@pub.guangzhou.gd.cn.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001343:Autophagy / Q000187:drug effects; D001393:Azoles / Q000494:pharmacology* / Q000627:therapeutic use; D016923:Cell Death / Q000187:drug effects*; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D005911:Gliosis / Q000473:pathology; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D054833:Isoindoles; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D016566:Organoselenium Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013788:Thalamus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; C042986:ebselen; D013482:Superoxide Dismutase
keywords: Autophagy; Cerebral infarction; Ebselen; Oxidative stress; Rats