title: Comparison between mixed lysate antigen and α-actinin antigen in ELISA for serodiagnosis of trichomoniasis.
abstract: The aim of this study was to identify an antigen suitable for ELISA for serodiagnosis of Trichomonas vaginalis (T. vaginalis) infection. Mixed lysate antigen (Ag) from eight strains of T. vaginalis and recombinant α-actinin protein was compared. The sera of three groups were examined by ELISA: 73 women infected with trichomoniasis served as a positive control, 31 male volunteers as a negative control, and 424 women attending an outpatient health screening at Hanyang University Guri Hospital. Based on the cutoff optical density for each Ag obtained with a negative control, the serosensitivity of the mixed lysate Ag (79.5%) was significantly higher than that of the α-actinin (52.1%) in the 73 patients with trichomoniasis. The specificity using lysate Ag and α-actinin was 100% and 96.8%, respectively. On the other hand, the positivity rate in 424 outpatients was 39.2% and 11.8% with mixed lysate and α-actinin Ag, respectively. Taken together, mixed lysate Ag showed higher sensitivity and specificity than α-actinin. Therefore, mixed lysate may be a better Ag than α-actinin for ELISA for the diagnosis of trichomoniasis.
authors.affiliation: Department of Obstetrics and Gynecology, Hanyang University Guri Hospital, College of Medicine, Gyeonggi Province 471-701, Republic of Korea.
authors.forename: Seung-Ryong
authors.initials: SR
authors.lastname: Kim
authors.affiliation: Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 133-791, Republic of Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Biochemistry & Molecular Biology, Hanyang University College of Medicine, Seoul 133-791, Republic of Korea.
authors.forename: Yong-Suk
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Preventive Medicine, College of Medicine, Dong-A University, Busan 602-715, Republic of Korea.
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Parasitology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Yeon-Chul
authors.initials: YC
authors.lastname: Hong
authors.affiliation: Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 133-791, Republic of Korea. Electronic address: jsryu@hanyang.ac.kr.
authors.forename: Jae-Sook
authors.initials: JS
authors.lastname: Ryu
mesh_terms: D000185:Actinin / Q000276:immunology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D016022:Case-Control Studies; D019411:Clinical Laboratory Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014247:Trichomonas Vaginitis / Q000097:blood / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000235:genetics / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000185:Actinin
keywords: ELISA; Lysate; Recombinant α-actinin; Serodiagnosis; Trichomonas vaginalis