title: A Pilot Study Identifying a Set of microRNAs As Precise Diagnostic Biomarkers of Acute Kidney Injury.
abstract: In the last decade, Acute Kidney Injury (AKI) diagnosis and therapy have not notably improved probably due to delay in the diagnosis, among other issues. Precocity and accuracy should be critical parameters in novel AKI biomarker discovery. microRNAs are key regulators of cell responses to many stimuli and they can be secreted to the extracellular environment. Therefore, they can be detected in body fluids and are emerging as novel disease biomarkers. We aimed to identify and validate serum miRNAs useful for AKI diagnosis and management. Using qRT-PCR arrays in serum samples, we determined miRNAs differentially expressed between AKI patients and healthy controls. Statistical and target prediction analysis allowed us to identify a panel of 10 serum miRNAs. This set was further validated, by qRT-PCR, in two independent cohorts of patients with relevant morbi-mortality related to AKI: Intensive Care Units (ICU) and Cardiac Surgery (CS). Statistical correlations with patient clinical parameter were performed. Our results demonstrated that the 10 selected miRNAs (miR-101-3p, miR-127-3p, miR-210-3p, miR-126-3p, miR-26b-5p, miR-29a-3p, miR-146a-5p, miR-27a-3p, miR-93-3p and miR-10a-5p) were diagnostic biomarkers of AKI in ICU patients, exhibiting areas under the curve close to 1 in ROC analysis. Outstandingly, serum miRNAs estimated before CS predicted AKI development later on, thus becoming biomarkers to predict AKI predisposition. Moreover, after surgery, the expression of the miRNAs was modulated days before serum creatinine increased, demonstrating early diagnostic value. In summary, we have identified a set of serum miRNAs as AKI biomarkers useful in clinical practice, since they demonstrate early detection and high diagnostic value and they recognize patients at risk.
authors.affiliation: miRNAs-based Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: Elia
authors.initials: E
authors.lastname: Aguado-Fraile
authors.affiliation: miRNAs-based Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: Edurne
authors.initials: E
authors.lastname: Ramos
authors.affiliation: miRNAs-based Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Conde
authors.affiliation: miRNAs-based Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: Macarena
authors.initials: M
authors.lastname: Rodríguez
authors.affiliation: miRNAs-based Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Martín-Gómez
authors.affiliation: Department of Intensive Medicine, University Hospital Ramón y Cajal, Carretera de Colmenar, Madrid, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Lietor
authors.affiliation: Department of Anesthesia and Reanimation, University Hospital Ramón y Cajal, Carretera de Colmenar, Madrid, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Candela
authors.affiliation: Department of Internal Medicine, University Hospital of Geneve, Geneva, Switzerland.
authors.forename: Belen
authors.initials: B
authors.lastname: Ponte
authors.affiliation: Department of Nephrology, University Hospital Ramón y Cajal, Madrid, Spain. Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Liaño
authors.affiliation: miRNAs-based Biomarkers and Therapeutic Targets Unit, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Carretera de Colmenar, Madrid, Spain.
authors.forename: María Laura
authors.initials: ML
authors.lastname: García-Bermejo
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000235:genetics*; D000328:Adult; D006348:Cardiac Surgical Procedures; D005260:Female; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D010865:Pilot Projects; D012372:ROC Curve
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs