title: Fluorescence and excited state dynamics of the deprotonated Schiff base retinal in proteorhodopsin.
abstract: The UV light absorbing species of proteorhodopsin with deprotonated Schiff base retinal was investigated using steady-state fluorescence and femtosecond pump-probe spectroscopy. Compared to the all-trans retinal with protonated Schiff base, the deprotonated chromophore absorbs at 365 nm and exhibits a blue-shifted fluorescence spectrum. The unusually long-lived excited state decays bi-exponentially with time constants of 8 ps and 130 ps to form a deprotonated 13-cis retinal as the primary photo-product.
authors.forename: Elena
authors.initials: E
authors.lastname: Bühl
authors.forename: Markus
authors.initials: M
authors.lastname: Braun
authors.forename: Andrea
authors.initials: A
authors.lastname: Lakatos
authors.forename: Clemens
authors.initials: C
authors.lastname: Glaubitz
authors.forename: Josef
authors.initials: J
authors.lastname: Wachtveitl
mesh_terms: D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D056004:Molecular Dynamics Simulation; D011522:Protons; D012172:Retinaldehyde / Q000737:chemistry*; D025604:Rhodopsins, Microbial / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D011522:Protons; D025604:Rhodopsins, Microbial; D012545:Schiff Bases; C413548:proteorhodopsin; D012172:Retinaldehyde