title: Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy.
abstract: When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF2 α. Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P4) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P4 concentrations during the maternal recognition period in cows.
authors.affiliation: Department of Animal ScienceTokyo University of Agriculture, Atsugi 243-0034, JapanGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanAnimal Feeding and Management Research DivisionNARO Institute of Livestock and Grassland Science, Nasushiobara 329-2793, JapanGraduate School of Environmental and Life ScienceOkayama University, Okayama 700-8530, JapanClinic for Reproduction MedicineUniversity of Zürich, Zurich, Switzerland.
authors.forename: Koumei
authors.initials: K
authors.lastname: Shirasuna
authors.affiliation: Department of Animal ScienceTokyo University of Agriculture, Atsugi 243-0034, JapanGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanAnimal Feeding and Management Research DivisionNARO Institute of Livestock and Grassland Science, Nasushiobara 329-2793, JapanGraduate School of Environmental and Life ScienceOkayama University, Okayama 700-8530, JapanClinic for Reproduction MedicineUniversity of Zürich, Zurich, Switzerland.
authors.forename: Haruka
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Department of Animal ScienceTokyo University of Agriculture, Atsugi 243-0034, JapanGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanAnimal Feeding and Management Research DivisionNARO Institute of Livestock and Grassland Science, Nasushiobara 329-2793, JapanGraduate School of Environmental and Life ScienceOkayama University, Okayama 700-8530, JapanClinic for Reproduction MedicineUniversity of Zürich, Zurich, Switzerland.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Matsuyama
authors.affiliation: Department of Animal ScienceTokyo University of Agriculture, Atsugi 243-0034, JapanGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanAnimal Feeding and Management Research DivisionNARO Institute of Livestock and Grassland Science, Nasushiobara 329-2793, JapanGraduate School of Environmental and Life ScienceOkayama University, Okayama 700-8530, JapanClinic for Reproduction MedicineUniversity of Zürich, Zurich, Switzerland.
authors.forename: Koji
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Department of Animal ScienceTokyo University of Agriculture, Atsugi 243-0034, JapanGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanAnimal Feeding and Management Research DivisionNARO Institute of Livestock and Grassland Science, Nasushiobara 329-2793, JapanGraduate School of Environmental and Life ScienceOkayama University, Okayama 700-8530, JapanClinic for Reproduction MedicineUniversity of Zürich, Zurich, Switzerland.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Bollwein
authors.affiliation: Department of Animal ScienceTokyo University of Agriculture, Atsugi 243-0034, JapanGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanAnimal Feeding and Management Research DivisionNARO Institute of Livestock and Grassland Science, Nasushiobara 329-2793, JapanGraduate School of Environmental and Life ScienceOkayama University, Okayama 700-8530, JapanClinic for Reproduction MedicineUniversity of Zürich, Zurich, Switzerland akiomiya@obihiro.ac.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D007370:Interferon Type I / Q000494:pharmacology*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D018375:Neutrophil Activation / Q000187:drug effects; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D019899:Paracrine Communication / Q000187:drug effects; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
chemical_list: D007370:Interferon Type I; D016209:Interleukin-8; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C020947:interferon tau; D011374:Progesterone