title: Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B.
abstract: Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson's disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection. The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway. To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD. Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN. We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively. This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion. However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures. Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model.
authors.affiliation: Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Batassini
authors.affiliation: Neuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, Brazil.
authors.forename: Núbia
authors.initials: N
authors.lastname: Broetto
authors.affiliation: Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Lucas Silva
authors.initials: LS
authors.lastname: Tortorelli
authors.affiliation: Neuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, Brazil.
authors.forename: Milene
authors.initials: M
authors.lastname: Borsoi
authors.affiliation: Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Zanotto
authors.affiliation: Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Galland
authors.affiliation: Neuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, Brazil.
authors.forename: Tadeu Mello
authors.initials: TM
authors.lastname: Souza
authors.affiliation: Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Marina Concli
authors.initials: MC
authors.lastname: Leite
authors.affiliation: Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, Brazil ; Neuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, Brazil.
authors.forename: Carlos-Alberto
authors.initials: CA
authors.lastname: Gonçalves
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000134:cerebrospinal fluid*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000134:cerebrospinal fluid* / Q000139:chemically induced / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit / Q000032:analysis / Q000134:cerebrospinal fluid*; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D064568:S100 Calcium Binding Protein beta Subunit; C579158:S100b protein, rat; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase