title: Flavonoids from Scutellaria attenuate okadaic acid-induced neuronal damage in rats.
abstract: OBJECTIVE
  To study the effect of flavonoids isolated from aerial parts of Scutellaria baicalensis Georgi (SSF) on cerebral damage induced by okadaic acid (OA) in rats.
  
  
  METHODS
  OA was microinjected into the right lateral ventricle of male rats at a dose of 200 ng kg(-1) twice with a 3-day interval between injections to establish a model of Alzheimer's-disease-like cerebral damage. Neuronal morphology was observed with thionin staining and the expressions of glial fibrillary acidic protein (GFAP) and β-amyloid peptide 1-40 (Aβ1-40) were monitored via immunohistochemistry. The level of malondialdehyde (MDA) and the activities of glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) were measured using spectrophotometry.
  
  
  RESULTS
  The results showed that OA-treated rats exhibited marked neuronal damage accompanied by increased levels of Aβ1-40 peptide and MDA accumulation, decreased GFAP protein expression and reduced GSH-Px and LDH activity in the brain. SSF at three doses (25, 50 and 100 mg kg(-1)) dramatically reversed the OA-induced changes in the brains of rats.
  
  
  CONCLUSIONS
  SSF-mediated amelioration of OA-induced neuronal damage in rats provides a rationale for assessing SSF as a means of to reducing tau hyperphosphorylation and Aβ expression in the treatment of Alzheimer's disease.
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zhang
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.|b Workers Hospital of Chengde Iron & Steel Group Co., Ltd , Chengde , Hebei , PR China.
authors.forename: Yong-Cai
authors.initials: YC
authors.lastname: Dong
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Wu
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Cheng
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Guan
authors.affiliation: a Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, Institute of Traditional Chinese Medicine, Chengde Medical College, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development , Chengde , Hebei , PR China and.
authors.forename: Ya-Zhen
authors.initials: YZ
authors.lastname: Shang
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D001930:Brain Injuries / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D005419:Flavonoids / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020547:Lateral Ventricles / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008845:Microinjections; D009474:Neurons / Q000187:drug effects*; D019319:Okadaic Acid; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D005419:Flavonoids; D005904:Glial Fibrillary Acidic Protein; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C528900:glial fibrillary astrocytic protein, mouse; D019319:Okadaic Acid; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase
keywords: Aβ1–40; GSH-Px; LDH; MDA; SSF; astrocyte; neuropathology; okadaic acid