title: MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine for Cancer Therapy.
abstract: Gene modification of eukaryotic cells by electroporation is a widely used method to express selected genes in a defined cell population for various purposes, like gene correction or production of therapeutics. Here, we describe the generation of a cell-based tumor vaccine via fourfold transient gene modification of a human renal cell carcinoma (RCC) cell line for high expression of CD80, CD154, GM-CSF, and IL-7 by use of MIDGE(®) vectors. The two co-stimulatory molecules CD80 and CD154 are expressed at the cell surface, whereas the two cytokines GM-CSF and IL-7 are secreted yielding cells with enhanced immunological properties. These fourfold gene-modified cells have been used as a cell-based tumor vaccine for the treatment of RCC.
authors.affiliation: Mologen AG Berlin, Fabeckstr. 30, 14195, Berlin, Germany, maschmidt@mologen.com.
authors.forename: Manuel
authors.initials: M
authors.lastname: Schmidt
authors.forename: Barbara
authors.initials: B
authors.lastname: Volz
authors.forename: Patrick
authors.initials: P
authors.lastname: Großmann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Heinrich
authors.forename: Burghardt
authors.initials: B
authors.lastname: Wittig
mesh_terms: D018122:B7-1 Antigen; D023201:CD40 Ligand / Q000378:metabolism; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D018274:Electroporation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D015851:Interleukin-7; D009369:Neoplasms / Q000276:immunology* / Q000628:therapy*; D013535:Suspensions; D014184:Transplantation, Homologous
chemical_list: D018122:B7-1 Antigen; D019496:Cancer Vaccines; D015851:Interleukin-7; D013535:Suspensions; D023201:CD40 Ligand; D016178:Granulocyte-Macrophage Colony-Stimulating Factor