title: Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD.
abstract: BACKGROUND
  Asymmetric dimethylarginine (ADMA) and nitric oxide (NO) show their mechanism of action reciprocally, the balance between these molecules contributes to the tight regulation of airways tone and function.
  
  
  OBJECTIVE
  The aim of this study to determine the serum levels of ADMA and NO in patients with chronic obstructive pulmonary disease (COPD) and establish whether their level vary in relation to forced expiratory volume in 1s (FEV1), to assess their role in pathophysiology of COPD.
  
  
  METHODS
  This study consisted of 58 patients with COPD and 30 healthy subjects. Serum ADMA and NO levels were measured using enzyme-linked immunosorbent assay and the colorimetric method, respectively.
  
  
  RESULTS
  Serum ADMA levels were significantly higher, however, NO levels were lower in patients with COPD compared with controls. ADMA levels were inversely correlated with NO levels. Serum ADMA and NO were significantly correlated with FEV1. Multivariable logistic regression analysis revealed that serum ADMA and NO were independently and significantly associated with the presence of COPD. Multiple linear regression analysis showed that COPD was positively associated with ADMA, additionally COPD and ADMA were independently and inversely associated with NO. NO levels were decreased, ADMA levels were increased compliant with progression of COPD stages.
  
  
  CONCLUSIONS
  While circulating ADMA is higher, NO is lower in COPD and both show a strong correlation to the degree of airflow limitation. ADMA seems to be a possible new marker of prognosis of COPD and can be a novel therapeutic target for the treatment of COPD.
authors.affiliation: Department of Biochemistry, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Aydin
authors.affiliation: Department of Pulmonary and Sleep Medicine, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Nejat
authors.initials: N
authors.lastname: Altintas
authors.affiliation: Department of Pulmonary and Sleep Medicine, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Cem Mutlu
authors.affiliation: Department of Internal Medicine, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Bilir
authors.affiliation: Department of Internal Medicine, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Oran
authors.affiliation: Department of Biochemistry, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Feti
authors.initials: F
authors.lastname: Tülübaş
authors.affiliation: Department of Biostatistics, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Birol
authors.initials: B
authors.lastname: Topçu
authors.affiliation: Department of Biochemistry, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Tayfur
authors.affiliation: Department of Biochemistry, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Volkan
authors.initials: V
authors.lastname: Küçükyalçin
authors.affiliation: Department of Pulmonary and Sleep Medicine, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Gizem
authors.initials: G
authors.lastname: Kaplan
authors.affiliation: Department of Biochemistry, School of Medicine, Namik Kemal University, Tekirdağ, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gürel
mesh_terms: D000368:Aged; D001120:Arginine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D003430:Cross-Sectional Studies; D018450:Disease Progression; D004791:Enzyme Inhibitors / Q000009:adverse effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D006801:Humans; D008168:Lung / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood / Q000172:deficiency*; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology*; D012129:Respiratory Function Tests; D012907:Smoking / Q000453:epidemiology
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; C487735:dimethylarginine; D009569:Nitric Oxide; C018524:N,N-dimethylarginine; D001120:Arginine
keywords: asymmetric dimethylarginine-chronic obstructive pulmonary disease-nitric oxide-pulmonary function test