title: BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development.
abstract: Previously, we showed that BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. In this study, we provide strong evidence that BCAS2 regulates the activity of Delta-Notch signaling via Delta pre-mRNA splicing. Depletion of dBCAS2 reduces Delta mRNA expression and leads to accumulation of Delta pre-mRNA, resulting in diminished transcriptions of Delta-Notch signaling target genes, such as cut and E(spl)m8. Furthermore, ectopic expression of human BCAS2 (hBCAS2) and Drosophila BCAS2 (dBCAS2) in a dBCAS2-deprived fly can rescue dBCAS2 depletion-induced wing damage to the normal phenotypes. These rescued phenotypes are correlated with the restoration of Delta pre-mRNA splicing, which affects Delta-Notch signaling activity. Additionally, overexpression of Delta can rescue the wing deformation by deprivation of dBCAS2; and the depletion of dBCAS2 can restore the aberrant eye associated with Delta-overexpressing retinas; providing supporting evidence for the regulation of Delta-Notch signaling by dBCAS2. Taken together, dBCAS2 participates in Delta pre-mRNA splicing that affects the regulation of Delta-Notch signaling in Drosophila wing development.
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Meng-Hsuan
authors.initials: MH
authors.lastname: Chou
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Chu-Wei
authors.initials: CW
authors.lastname: Huang
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Po-Han
authors.initials: PH
authors.lastname: Chen
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Shih-Peng
authors.initials: SP
authors.lastname: Chan
authors.affiliation: Department of Ophthalmology, Mackay Memorial Hospital, Taipei, 104, Taiwan.
authors.forename: Yeou-Ping
authors.initials: YP
authors.lastname: Tsao
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Hsiu-Hsiang
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan; Department of Medical Research, National Taiwan University Hospital, Taipei, 100, Taiwan.
authors.forename: June-Tai
authors.initials: JT
authors.lastname: Wu
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Show-Li
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000818:Animals; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism
chemical_list: C579021:BCAS2 protein, Drosophila; D029721:Drosophila Proteins; D009363:Neoplasm Proteins; D012322:RNA Precursors; D051880:Receptors, Notch