title: A Rationally Designed Thymidine-Based Self-Assembled Monolayer on a Gold Electrode for Electroanalytical Applications.
abstract: A self-assembled monolayer (SAM) of 1-(3,5-epidithio-2,3,5-trideoxy-β-D-threo-pentofuranosyl)thymine (EFT) on a gold electrode was prepared and characterized by Raman spectral and electrochemical measurements. Voltammetric and electrochemical impedance measurements show that the SAM of EFT on a Au electrode impedes the electron-transfer reaction. The SAM of EFT was successfully used for the voltammetric sensing of urate in neutral solution. The coexisting ascorbate anion does not interfere and therefore the EFT-based electrode was able to quantify urate at the micromolar level in the presence of a large excess amount of ascorbate. To demonstrate the practical applications, the amount of urate in two different human serum samples was quantified by using the EFT-based electrode; the results are in good agreement with those determined by the clinical method. DFT calculations show that both ascorbate and urate have noncovalent interactions including hydrogen-bonding interactions with EFT.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Datta
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Bera
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Saibal
authors.initials: S
authors.lastname: Jana
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Manna
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Debayan
authors.initials: D
authors.lastname: Roy
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Anakuthil
authors.initials: A
authors.lastname: Anoop
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India. crraj@chem.iitkgp.ernet.in.
authors.forename: C Retna
authors.initials: CR
authors.lastname: Raj
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India. tpathak@chem.iitkgp.ernet.in.
authors.forename: Tanmaya
authors.initials: T
authors.lastname: Pathak
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D008958:Models, Molecular; D013936:Thymidine / Q000737:chemistry*; D014527:Uric Acid / Q000032:analysis / Q000097:blood*
chemical_list: D014527:Uric Acid; D006046:Gold; D001205:Ascorbic Acid; D013936:Thymidine
keywords: gold; hydrogen bonds; monolayers; self-assembly; sensors