title: Urinary N telopeptide levels in predicting the anti-nociceptive responses of zoledronic acid and paclitaxel in a rat model of bone metastases.
abstract: The present study investigated the hypothesis that urinary levels of N telopeptide (NTx) can be used to predict the anti‑nociceptive responses of zoledronic acid and paclitaxel on bone metastases in a rat model. Rats were implanted with intra‑femur Walker 256 carcinoma cells or control solution, and were treated with either normal saline, zoledronic acid or paclitaxel on the 10th day following surgery. Mechanical allodynia was recorded and the urine collagen‑crosslinked NTx values were measured prior to, and 7, 14 and 21 days following the injections. Bone sections and osteoclasts were stained on the 14th day (4 days post‑injection). Furthermore, the mRNA and protein expression levels of c‑fos in the spinal cord and acid‑sensing ion channel 3 (ASIC3) in the dorsal root ganglion (DRG) were analyzed. The mechanical allodynia of rats was attenuated from day 14 in the zoledronic acid group and from day 21 in the paclitaxel group. A positive correlation was observed between the anti‑nociceptive responses of zoledronic acid and paclitaxel, and the urinary levels of NTx (r=0.619; P<0.001). The mRNA levels of c‑fos in the spinal cord and ASIC3 in the DRG in the zoledronic acid group were reduced 14 and 21 days after inoculation, and this reduction was observed in the paclitaxel group 21 days after inoculation. Low dose paclitaxel was observed to have a weaker anti‑nociceptive effect on bone cancer pain, with a later‑onset, compared with zoledronic acid. The results suggested that urinary levels of NTx may predict the anti‑nociceptive responses of zoledronic acid and paclitaxel in a rat model of bone metastases.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gui
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhuang
authors.affiliation: Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
authors.forename: Shu
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Yu
mesh_terms: D062565:Acid Sensing Ion Channels / Q000378:metabolism; D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D015415:Biomarkers / Q000652:urine; D001859:Bone Neoplasms / Q000556:secondary / Q000652:urine*; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000652:urine*; D004164:Diphosphonates / Q000494:pharmacology* / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D005260:Female; D005727:Ganglia, Spinal; D006930:Hyperalgesia / Q000188:drug therapy / Q000652:urine; D007093:Imidazoles / Q000494:pharmacology* / Q000627:therapeutic use; D009368:Neoplasm Transplantation; D010010:Osteoclasts / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology* / Q000627:therapeutic use; D010146:Pain / Q000188:drug therapy* / Q000652:urine; D010455:Peptides / Q000652:urine*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism; D000077211:Zoledronic Acid
chemical_list: C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; D000700:Analgesics; D015415:Biomarkers; D024042:Collagen Type I; D004164:Diphosphonates; D007093:Imidazoles; D010455:Peptides; D016760:Proto-Oncogene Proteins c-fos; C052929:collagen type I trimeric cross-linked peptide; D000077211:Zoledronic Acid; D017239:Paclitaxel