title: An UPLC-MS/MS method for the quantitation of vortioxetine in rat plasma: Application to a pharmacokinetic study.
abstract: In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of vortioxetine in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) column with a gradient mobile phase consisting of 0.1% formic acid in water and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.05-20ng/mL (R(2)>0.997) with a lower limit of quantification (0.05ng/mL). The extraction recovery was in the range of 78.3-88.4% for vortioxetine and 80.3% for carbamazepine (internal standard, IS). The intra- and inter-day precision was below 8.5% and accuracy was from -11.2% to 9.5%. No notable matrix effect and astaticism was observed for vortioxetine. The method has been successfully applied to a pharmacokinetic study of vortioxetine in rats for the first time, which provides the basis for the further development and application of vortioxetine.
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Er-min
authors.initials: EM
authors.lastname: Gu
authors.affiliation: The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Liang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Lingjing
authors.initials: L
authors.lastname: Yuan
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Lan
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: hgx@wmu.edu.cn.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Hu
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: wzmuzhouhy@126.com.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000078784:Vortioxetine
chemical_list: D010879:Piperazines; D013440:Sulfides; D000078784:Vortioxetine
keywords: Pharmacokinetics; Plasma; Rat; UPLC–MS/MS; Vortioxetine