title: Gene expression profiling leads to discovery of correlation of matrix metalloproteinase 11 and heparanase 2 in breast cancer progression.
abstract: BACKGROUND
  In order to identify biomarkers involved in breast cancer, gene expression profiling was conducted using human breast cancer tissues.
  
  
  METHODS
  Total RNAs were extracted from 150 clinical patient tissues covering three breast cancer subtypes (Luminal A, Luminal B, and Triple negative) as well as normal tissues. The expression profiles of a total of 50,739 genes were established from a training set of 32 samples using the Agilent Sure Print G3 Human Gene Expression Microarray technology. Data were analyzed using Agilent Gene Spring GX 12.6 software. The expression of several genes was validated using real-time RT-qPCR.
  
  
  RESULTS
  Data analysis with Agilent GeneSpring GX 12.6 software showed distinct expression patterns between cancer and normal tissue samples. A group of 28 promising genes were identified with ≥ 10-fold changes of expression level and p-values < 0.05. In particular, MMP11 and HPSE2 were closely examined due to the important roles they play in cancer cell growth and migration. Real-time RT-qPCR analyses of both training and testing sets validated the gene expression profiles of MMP11 and HPSE2.
  
  
  CONCLUSIONS
  Our findings identified these 2 genes as a novel breast cancer biomarker gene set, which may facilitate the diagnosis and treatment in breast cancer clinical therapies.
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Florida, 1600 SW Archer Rd, Health Science Center P5-31, Gainesville, FL, 32610, USA. jfu@ufl.edu.
authors.forename: Junjie
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Florida, 1600 SW Archer Rd, Health Science Center P5-31, Gainesville, FL, 32610, USA. ravilx84@gmail.com.
authors.forename: Ravil
authors.initials: R
authors.lastname: Khaybullin
authors.affiliation: Gene Expression and Genotyping, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA. yanp@ad.ufl.edu.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Columbia University, New York, NY, 10027, USA. ax2118@columbia.edu.
authors.forename: Amy
authors.initials: A
authors.lastname: Xia
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Florida, 1600 SW Archer Rd, Health Science Center P5-31, Gainesville, FL, 32610, USA. xqi@cop.ufl.edu.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
mesh_terms: D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D016000:Cluster Analysis; D019295:Computational Biology; D030561:Databases, Nucleic Acid; D005260:Female; D020869:Gene Expression Profiling; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D053507:Matrix Metalloproteinase 11 / Q000235:genetics*; D015203:Reproducibility of Results; D059467:Transcriptome; D000099175:Heparanase
chemical_list: D014408:Biomarkers, Tumor; D005966:Glucuronidase; D053507:Matrix Metalloproteinase 11; D000099175:Heparanase