title: Interaction between Polymorphisms in Pre-MiRNA Genes and Cooking Oil Fume Exposure on the Risk of Lung Cancer in Chinese Non-Smoking Female Population.
abstract: BACKGROUND
  Both genetic polymorphisms and environmental risk factors play important roles in the development of human chronic diseases including lung cancer. This is the first case-control study of interaction between polymorphisms in pre-miRNA genes and cooking oil fume exposure on the risk of lung cancer.
  
  
  METHODS
  A hospital-based case-control study of 258 cases and 310 controls was conducted. Six polymorphisms in miRNAs were determined by Taqman allelic discrimination method. The gene-environment interactions were assessed on both additive and multiplicative scale. The statistical analyses were performed mostly with SPSS.
  
  
  RESULTS
  The combination of the risk genotypes of five miRNA SNPs (miR-146a rs2910164, miR-196a2 rs11614913, miR-608 rs4919510, miR-27a rs895819 and miR-423 rs6505162) with risk factor (cooking oil fume exposure) contributed to a significantly higher risk of lung cancer, and the corresponding ORs (95% confidence intervals) were 1.91(1.04-3.52), 1.94 (1.16-3.25), 2.06 (1.22-3.49), 1.76 (1.03-2.98) and 2.13 (1.29-3.51). The individuals with both risk genotypes of miRNA SNPs and exposure to risk factor (cooking oil fumes) were in a higher risk of lung cancer than persons with only one of the two risk factors (ORs were 1.91, 1.05 and 1.41 for miR-146a rs2910164, ORs were 1.94, 1.23 and 1.34 for miR-196a2 rs11614913, ORs were 2.06, 1.41 and 1.68 for miR-608 rs4919510, ORs were 1.76, 0.82 and 1.07 for miR-27a rs895819, and ORs were 2.13, 1.15 and 1.02 for miR-423 rs6505162, respectively). All the measures of biological interaction indicate that there were not indeed biological interactions between the six SNPs of miRNAs and exposure to cooking oil fumes on an additive scale. Logistic models suggested that the gene-environment interactions were not statistically significant on a multiplicative scale.
  
  
  CONCLUSIONS
  The interactions between miRNA SNPs and cooking oil fume exposure suggested by ORs of different combination were not statistically significant.
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110013, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yin
authors.affiliation: China Medical University, Shenyang 110013, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110013, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guan
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110013, PR China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110013, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110013, PR China.
authors.forename: Yangwu
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China.
authors.forename: Qincheng
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110013, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110013, PR China.
authors.forename: Baosen
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000209:etiology; D000368:Aged; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D002681:China; D003296:Cooking; D004198:Disease Susceptibility; D005260:Female; D005740:Gases / Q000737:chemistry*; D005838:Genotype; D006801:Humans; D007407:Interviews as Topic; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000209:etiology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D016017:Odds Ratio; D009821:Oils / Q000009:adverse effects* / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D005740:Gases; D035683:MicroRNAs; D009821:Oils