title: Ursolic acid derivative ameliorates streptozotocin-induced diabestic bone deleterious effects in mice.
abstract: OBJECTIVE
  This study was performed to investigate bone deteriorations of diabetic mice in response to the treatment of ursolic acid derivative (UAD).
  
  
  METHODS
  The biomarkers in serum and urine were measured, tibias were taken for the measurement on gene and protein expression and histomorphology analysis, and femurs were taken for the measurement on bone Ca and three-dimensional architecture of trabecular bone.
  
  
  RESULTS
  UAD showed a greater increase in bone Ca, BMD and significantly increased FGF-23 and OCN, reduced PTH and CTX in diabetic mice. UAD reversed STZ-induced trabecular deleterious effects and stimulated bone remodeling. The treatment of STZ group with UAD significantly elevated the ratio of OPG/RANKL. Moreover, insulin and IGF-1 showed a negative correlation with both FBG and Hb1Ac in STZ group. We attributed down-regulating the level of Hb1Ac in diabetic mice to that ursolic acid derivative could primely control blood sugar levels. After analyzing of two adipocyte markers, PPARγ and aP2, increased expression in the tibias of diabetic mice, and UAD could improve STZ-induced adipocyte dysfunction.
  
  
  CONCLUSIONS
  These results demonstrated that UAD could ameliorate STZ-induced bone deterioration through improving adipocyte dysfunction and enhancing new bone formation and inhibiting absorptive function of osteoclast in the bone of diabetic mice.
authors.affiliation: Department of Endocrinology, Affiliated Hosptial of Binzhou Medical University Binzhou 256603, China.
authors.forename: Su-Guo
authors.initials: SG
authors.lastname: Yu
authors.affiliation: Department of Emergency, People's Hospital of Binzhou Binzhou 256610, China.
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Hospital of Yangxin Traditional Chinese Medicine Binzhou 251880, China.
authors.forename: Xiu-E
authors.initials: XE
authors.lastname: Xu
authors.affiliation: Department of Endocrinology, Affiliated Hosptial of Binzhou Medical University Binzhou 256603, China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, Affiliated Hosptial of Binzhou Medical University Binzhou 256603, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Affiliated Hosptial of Binzhou Medical University Binzhou 256603, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Yu
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005269:Femur / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D000089703:Fibroblast Growth Factor-23; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053244:Osteoprotegerin / Q000097:blood; D053245:RANK Ligand / Q000097:blood; D013977:Tibia / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D002118:Calcium; D000089703:Fibroblast Growth Factor-23; D007328:Insulin; D007334:Insulin-Like Growth Factor I; D053244:Osteoprotegerin; D053245:RANK Ligand; D014315:Triterpenes; D000097245:Ursolic Acid; C000717428:Fgf23 protein, mouse
keywords: OPG/RANKL; PPARγ; bone; diabetic; ursolic acid derivative