title: Metabonomic Evaluation of Chronic Unpredictable Mild Stress-Induced Changes in Rats by Intervention of Fluoxetine by HILIC-UHPLC/MS.
abstract: Hydrophilic interaction-ultra high performance liquid chromatography (HILIC-UHPLC) allows the analysis of highly polar metabolites, providing complementary information to reversed-phase (RP) chromatography. By optimization of the preparation and analytical conditions in HILIC mode, HILIC-UHPLC/MS was applied for the global metabolic profiling of rat plasma samples generated in an experimental model of chronic unpredictable mild stress (CUMS), and the concomitant investigation of the protective effect of fluoxetine was also evaluated. Identification of plasma metabolic profiles indicated that significant changes in specific metabolites occurred after fluoxetine exposure, including increased phenylalanine, serine, acetyl-L-carnitine, carnitine and decreased creatine, betaine, proline, tryptophan, tyrosine, C16:0 LPC. Some novel biomarkers from this HILIC-UHPLC/MS approach were betaine, proline, tyrosine creatine and serine compared with the results of RP-UHPLC/MS. The complementary nature of this technique is confirmed and is on agreement with previously published studies.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Lu
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.
authors.forename: Shuning
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ge
authors.affiliation: Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Guangyue
authors.initials: G
authors.lastname: Su
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning China.
authors.forename: Chunfu
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003863:Depression / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D005473:Fluoxetine / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D018687:Antidepressive Agents, Second-Generation; D015415:Biomarkers; D005473:Fluoxetine