title: Intestinal B(0)AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources.
abstract: The objective of the present study was to examine the effect of diets with descending fish meal (FM) inclusion levels and the addition of salt to the diet containing the lowest FM level on growth performances, feed conversion ratio, and intestinal solute carrier family 6 member 19 (SLC6A19) and oligopeptide transporter 1 (PEPT1) transcript levels, in freshwater-adapted European sea bass (Dicentrarchus labrax). We first isolated by molecular cloning and sequenced a full-length cDNA representing the neutral amino acid transporter SLC6A19 in sea bass. The cDNA sequence was deposited in GenBank database (accession no. KC812315). The twelve transmembrane domains and the 'de novo' prediction of the three-dimensional structure of SLC6A19 protein (634 amino acids) are presented. We then analysed diet-induced changes in the mRNA copies of SLC6A19 and PEPT1 genes in different portions of sea bass intestine using real-time RT-PCR. Sea bass were fed for 6 weeks on different diets, with ascending levels of fat or descending levels of FM, which was replaced with vegetable meal. The salt-enriched diet was prepared by adding 3 % NaCl to the diet containing 10 % FM. SLC6A19 mRNA in the anterior and posterior intestine of sea bass were not modulated by dietary protein sources and salt supplementation. Conversely, including salt in a diet containing a low FM percentage up-regulated the mRNA copies of PEPT1 in the hindgut. Fish growth correlated positively with the content of FM in the diets. Interestingly, the addition of salt to the diet containing 10 % FM improved feed intake, as well as specific growth rate and feed conversion ratio.
authors.affiliation: Department of Biotechnology and Life Sciences , University of Insubria , 21100 Varese , Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Rimoldi
authors.affiliation: Department of Biotechnology and Life Sciences , University of Insubria , 21100 Varese , Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Bossi
authors.affiliation: Agricultural Research Organization, The Volcani Center , Bet Dagan 50250 , Israel.
authors.forename: Sheenan
authors.initials: S
authors.lastname: Harpaz
authors.affiliation: Department of Biotechnology and Life Sciences , University of Insubria , 21100 Varese , Italy.
authors.forename: Anna Giulia
authors.initials: AG
authors.lastname: Cattaneo
authors.affiliation: Department of Biotechnology and Life Sciences , University of Insubria , 21100 Varese , Italy ; Inter-University Centre for Research in Protein Biotechnologies , "The Protein Factory" , Polytechnic University of Milan and University of Insubria , Varese , Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bernardini
authors.affiliation: Department of Biotechnology and Life Sciences , University of Insubria , 21100 Varese , Italy ; Inter-University Centre for Research in Protein Biotechnologies , "The Protein Factory" , Polytechnic University of Milan and University of Insubria , Varese , Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Saroglia
authors.affiliation: Department of Biotechnology and Life Sciences , University of Insubria , 21100 Varese , Italy ; Inter-University Centre for Research in Protein Biotechnologies , "The Protein Factory" , Polytechnic University of Milan and University of Insubria , Varese , Italy.
authors.forename: Genciana
authors.initials: G
authors.lastname: Terova
keywords: ACE2, angiotensin-converting enzyme 2; Aquaculture; Dietary salt addition; FCR, feed conversion ratio; FM, fish meal; Fish meal replacement; Gene expression; PEPT1, oligopeptide transporter 1; RACE, rapid amplification of the cDNA ends; Real-time PCR; SBM, soyabean meal; SGR, specific growth rate; SLC6A19, solute carrier family 6 member 19; Vegetable meal