title: A sensitive, rapid, and simple DR-EcoScreen bioassay for the determination of PCDD/Fs and dioxin-like PCBs in environmental and food samples.
abstract: In developing countries in Asia, such as China, Vietnam, and Thailand, there is a strong need for the development of relatively rapid and low-cost bioassays for the determination of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (DL-PCBs) in environmental and food samples. These compounds are known to induce a variety of toxic and biological effects through their ligand-specific binding of the aryl hydrocarbon receptor (AhR). Indeed, several AhR-mediated reporter gene assays are widely used as prescreening tools for high-resolution gas chromatography/high-resolution mass spectrometry (HRGC-HRMS) analysis, which individually measures 17 PCDD/Fs and 12 DL-PCBs. In 2008, we have developed a new sensitive and rapid reporter gene assay using a genetically engineered stable cell line, designated DR-EcoScreen cells. The DR-EcoScreen assay using these cells has a number of great advantages of its sensitivity to 2,3,7,8-tetrachlorodibenzo-p-dioxin and its simple procedure, which shows little variance in the data (within CV 10 %) compared to other reporter gene assays. In this review, we summarize the application of the DR-EcoScreen assay to the determination of PCDD/Fs and DL-PCBs in ambient air samples, in fish and shellfish samples, and in flue gas samples from incinerators and provide potential usefulness of this bioassay for the determination of PCDD/Fs and DL-PCBs in various matrices.
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo, 060-0819, Japan. kojima@iph.pref.hokkaido.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kojima
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo, 060-0819, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeuchi
authors.affiliation: Otsuka Pharmaceutical Company Ltd, 224-18 Ebisuno Hiraishi, Kawauchi-cho, Tokushima, 771-0195, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Iida
authors.affiliation: National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.
authors.forename: Shoji F
authors.initials: SF
authors.lastname: Nakayama
authors.affiliation: Environmental Sanitation Center, 10-6 Yotsuyakami-cho, Kawasaki-ku, Kawasaki, 210-0828, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Shiozaki
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000191:economics / Q000379:methods*; D002460:Cell Line; D000072338:Dibenzofurans, Polychlorinated / Q000032:analysis; D004784:Environmental Monitoring / Q000191:economics / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D005504:Food Analysis / Q000191:economics / Q000379:methods*; D017930:Genes, Reporter; D011078:Polychlorinated Biphenyls / Q000032:analysis; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis
chemical_list: D000072338:Dibenzofurans, Polychlorinated; D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D011078:Polychlorinated Biphenyls
keywords: Ambient air; Aryl hydrocarbon receptor; Dioxin-like PCBs; Fish; Flue gas; PCDDs/Fs; Reporter gene assay