title: Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection.
abstract: The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP) 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an innocuous replication-defective bovine adenovirus serotype 3 (BAdV3) in a controlled laboratory environment. The ASAP efficiently trapped the surrogate virus at 5 × 10(3) plaque-forming units (p.f.u.) [2 × 10(5) genome copy equivalent] concentrations or more resulting in the successful detection of the virus using quantitative PCR. These results support the further development of ASAP for bioaerosol pathogen detection.
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette IN, USA.
authors.forename: Anurag
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette IN, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Clark
authors.affiliation: School of Health Sciences, College of Health and Human Sciences, Regenstrief Center for Healthcare Engineering, Purdue University, West Lafayette IN, USA.
authors.forename: James D
authors.initials: JD
authors.lastname: McGlothlin
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette IN, USA.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Mittal
keywords: air samples; airborne pathogens; ambient air sampler; bioaerosol; bioterrorism; pandemic; pathogen detection; virus detection