title: PCR melting profile as a tool for outbreak studies of Salmonella enterica in chickens.
abstract: BACKGROUND
  Salmonellosis is of great economic concern in all phases of the poultry industry, from production to marketing, leading to severe economic losses. Monitoring the source of the bacterial contamination has fundamental importance in the spreading of salmonellosis.
  
  
  RESULTS
  We applied a ligation-mediated PCR method, PCR MP (PCR melting profile), to type S. enterica ssp. enterica ser. Enteritidis (56 strains) and 43 control strains classified to other serovars isolated from poultry. We demonstrated the PCR MP potential for salmonellosis spreading monitoring. Our rapid test presents higher discriminatory power (0.939 vs. 0.608) compared to current molecular subtyping tool such as pulsed-field gel electrophoresis (PFGE), which ineffectiveness underlies the high degree of clonality of S. Enteritidis.
  
  
  CONCLUSIONS
  PCR MP was found to be a highly discriminating, sensitive and specific method that could be a valuable molecular tool, particularly for analyzing epidemiological links of limited number of S. enterica ser. Enteritidis strains.
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszów, Rejtana 16c, 35-959, Rzeszów, Poland. ania.zaczek@yahoo.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Zaczek
authors.affiliation: Proteon Pharmaceuticals S.A., Tylna 3, 90-364, Łódź, Poland. awojtasik@proteonpharma.com.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Wojtasik
authors.affiliation: Molecular Microbiology Department, National Medicines Institute, Chełmska 30/34, 00-725, Warsaw, Poland. radekizdebski@yahoo.com.
authors.forename: Radosław
authors.initials: R
authors.lastname: Izdebski
authors.affiliation: Proteon Pharmaceuticals S.A., Tylna 3, 90-364, Łódź, Poland. egorecka@proteonpharma.com.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Gorecka
authors.affiliation: Proteon Pharmaceuticals S.A., Tylna 3, 90-364, Łódź, Poland. ewojcik@proteonpharma.com.
authors.forename: Ewelina A
authors.initials: EA
authors.lastname: Wojcik
authors.affiliation: Vet- Lab Brudzew, Turkowska 58C, 62-720, Brudzew, Poland. tnowak@poczta.onet.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Nowak
authors.affiliation: Vet- Lab Brudzew, Turkowska 58C, 62-720, Brudzew, Poland. lab.brudzew@interia.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kwiecinski
authors.affiliation: Institute of Medical Biology, Polish Academy of Science, Lodowa 106, 93-232, Łódź, Poland. jdziadek@toya.net.pl.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Dziadek
mesh_terms: D000818:Animals; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D009691:Nucleic Acid Denaturation / Q000235:genetics*; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D019779:Salmonella enterica / Q000235:genetics*