title: Barley Hv CIRCADIAN CLOCK ASSOCIATED 1 and Hv PHOTOPERIOD H1 Are Circadian Regulators That Can Affect Circadian Rhythms in Arabidopsis.
abstract: Circadian clocks regulate many aspects of plant physiology and development that contribute to essential agronomic traits. Circadian clocks contain transcriptional feedback loops that are thought to generate circadian timing. There is considerable similarity in the genes that comprise the transcriptional and translational feedback loops of the circadian clock in the plant Kingdom. Functional characterisation of circadian clock genes has been restricted to a few model species. Here we provide a functional characterisation of the Hordeum vulgare (barley) circadian clock genes Hv circadian clock associated 1 (HvCCA1) and Hv photoperiodh1, which are respectively most similar to Arabidopsis thaliana circadian clock associated 1 (AtCCA1) and pseudo response regulator 7 (AtPRR7). This provides insight into the circadian regulation of one of the major crop species of Northern Europe. Through a combination of physiological assays of circadian rhythms in barley and heterologous expression in wild type and mutant strains of A. thaliana we demonstrate that HvCCA1 has a conserved function to AtCCA1. We find that Hv photoperiod H1 has AtPRR7-like functionality in A. thaliana and that the effects of the Hv photoperiod h1 mutation on photoperiodism and circadian rhythms are genetically separable.
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, United Kingdom.
authors.forename: Jelena
authors.initials: J
authors.lastname: Kusakina
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom; National Institute of Agricultural Botany, Cambridge, United Kingdom.
authors.forename: Zoe
authors.initials: Z
authors.lastname: Rutterford
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, United Kingdom.
authors.forename: Sean
authors.initials: S
authors.lastname: Cotter
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom.
authors.forename: María C
authors.initials: MC
authors.lastname: Martí
authors.affiliation: John Innes Centre, Coney Lane, Norwich, United Kingdom.
authors.forename: David A
authors.initials: DA
authors.lastname: Laurie
authors.affiliation: National Institute of Agricultural Botany, Cambridge, United Kingdom.
authors.forename: Andy J
authors.initials: AJ
authors.lastname: Greenland
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, United Kingdom.
authors.forename: Anthony
authors.initials: A
authors.lastname: Hall
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom.
authors.forename: Alex A R
authors.initials: AA
authors.lastname: Webb
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D057906:Circadian Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D005816:Genetic Complementation Test; D001467:Hordeum / Q000502:physiology*; D009154:Mutation; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C114425:CCA1 protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors