title: Increasing yield of nanocrystalline cellulose preparation process by a cellulase pretreatment.
abstract: In this work the introduction of a cellulase treatment prior to NCC isolation was assessed. NCC was produced using sulfuric acid at two different concentrations (62 and 64% wt.). The effect of pore size for filtration step was also assessed. The smaller acid dose leaded to yields up to 65-70% and average size up to 160 nm. It also produced crystals with reduced sulfur content (0.6-1%). Cellulase pretreatment influenced NCC characteristics, as it increased overall yield a 12%, increased average particle size around 35 nm and reduced NCC sulfur content up to a 0.8%. We found that different conditions of enzymatic treatments led to quantitative differences on their effects on NCC. Acetate buffer used for enzymatic treatments was found to counteract effects of acid. The evidence presented in this work suggested that pretreating fibers with this cellulase represents a very interesting option to partially replace chemicals on NCC isolation.
authors.affiliation: CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
authors.forename: Facundo
authors.initials: F
authors.lastname: Beltramino
authors.affiliation: CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain. Electronic address: roncero@etp.upc.edu.
authors.forename: M Blanca
authors.initials: MB
authors.lastname: Roncero
authors.affiliation: CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Vidal
authors.affiliation: CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Torres
authors.affiliation: CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Valls
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000138:chemical synthesis*; D003460:Crystallization / Q000379:methods*; D003368:Gossypium / Q000737:chemistry*; D006868:Hydrolysis; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D013464:Sulfuric Acids; D002482:Cellulose; D002480:Cellulase; C033158:sulfuric acid
keywords: Acid hydrolysis; Cellulase; Enzymatic treatment; Nanocrystalline cellulose; Yield increase