title: Controlled Delivery of Human Cells by Temperature Responsive Microcapsules.
abstract: Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into. Encapsulation of individual cells has been proposed as a means of increasing cell viability. In this study, we developed a facile, high throughput method for creating temperature responsive microcapsules comprising agarose, gelatin and fibrinogen for delivery and subsequent controlled release of cells. We verified the hypothesis that composite capsules combining agarose and gelatin, which possess different phase transition temperatures from solid to liquid, facilitated the destabilization of the capsules for cell release. Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line-human umbilical vein endothelial cells (HUVECs). While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. mamak@ifm.liu.se.|Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linkӧping University, SE58183, Linköping, Sweden. mamak@ifm.liu.se.
authors.forename: W C
authors.initials: WC
authors.lastname: Mak
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. kim.olesen@liu.se.
authors.forename: K
authors.initials: K
authors.lastname: Olesen
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. petter.sivler@s2m.se.
authors.forename: P
authors.initials: P
authors.lastname: Sivlér
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. chyanjang@gmail.com.
authors.forename: C J
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. inesmorenojimenez@gmail.com.|Bone & Joint Research Group, Stem Cells & Regeneration Institute of Developmental Sciences, Southampton General Hospital, Southampton, Hampshire SO16 6YD, UK. inesmorenojimenez@gmail.com.
authors.forename: I
authors.initials: I
authors.lastname: Moreno-Jimenez
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. joel.edin@liu.se.
authors.forename: J
authors.initials: J
authors.lastname: Edin
authors.affiliation: Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario K1H 8L6, Canada. dcourtman@ohri.ca.
authors.forename: D
authors.initials: D
authors.lastname: Courtman
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. marten.skog@liu.se.
authors.forename: M
authors.initials: M
authors.lastname: Skog
authors.affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. may.griffith@liu.se.
authors.forename: M
authors.initials: M
authors.lastname: Griffith
keywords: cell delivery; cell encapsulation; human fibroblast; human umbilical vein endothelial cells; hydrogel; microcapsules; temperature responsive