title: Combined fiber probe for fluorescence lifetime and Raman spectroscopy.
abstract: In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated. Graphical Abstract An image comparison between FLIm and Raman spectroscopy acquired with the bimodal probe onseveral tissue samples.
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Dochow
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA, 95616, USA.
authors.forename: Dinglong
authors.initials: D
authors.lastname: Ma
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Latka
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bocklitz
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA, 95616, USA.
authors.forename: Brad
authors.initials: B
authors.lastname: Hartl
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA, 95616, USA.
authors.forename: Julien
authors.initials: J
authors.lastname: Bec
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA, 95616, USA.
authors.forename: Hussain
authors.initials: H
authors.lastname: Fatakdawala
authors.affiliation: EmVision LCC, 1471 F Road, Loxahatchee, FL, 33470, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Marple
authors.affiliation: EmVision LCC, 1471 F Road, Loxahatchee, FL, 33470, USA.
authors.forename: Kirk
authors.initials: K
authors.lastname: Urmey
authors.affiliation: Department of Pathology and Laboratory Medicine and Center for Biophotonics, University of California, Davis, 2700 Stockton Blvd., Sacramento, CA, 95817, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wachsmann-Hogiu
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmitt
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, 451 E. Health Sciences Drive, Davis, CA, 95616, USA. lmarcu@ucdavis.edu.
authors.forename: Laura
authors.initials: L
authors.lastname: Marcu
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany. juergen.popp@uni-jena.de.|Leibniz Institute of Photonic Technology Jena e.V., Albert-Einstein-Str. 9, 07745, Jena, Germany. juergen.popp@uni-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D004724:Endoscopy / Q000295:instrumentation; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D061848:Optical Imaging / Q000295:instrumentation*; D011916:Rats, Inbred F344; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*
keywords: Bimodal; Fiber probes; Fluorescence lifetime; Imaging; Raman spectroscopy