title: A functional polymorphism in the promoter region of interleukin-10 gene increases the risk for spontaneous abortions--a triad study.
abstract: OBJECTIVE
  Spontaneous abortion or miscarriage is the natural death of an embryo or foetus in the early stages of prenatal development. Interleukin-10 is an anti-inflammatory cytokine, produced by human cytotrophoblasts, and defects in its production result in specific pathological conditions during pregnancy. The present study is aimed to evaluate the association of IL-10 -1082G/A polymorphism in spontaneous abortions by comparing foetal, maternal and paternal groups--a triad study.
  
  
  METHODS
  A total of 50 families with spontaneous abortions and 60 families with medically terminated pregnancies were considered for the present study. DNA from foetal tissue and parental blood samples were extracted, and the genotype analysis of IL-10 -1082G/A promoter polymorphism was carried out by amplification refractory mutation system-polymerase chain reaction followed by agarose gel electrophoresis. A statistical analysis was applied to test for the significance of the results.
  
  
  RESULTS
  There was a statistically significant difference in the distribution of AA genotypes and A allele of IL-10 -1082G/A between the two family groups among foetuses (P = 0.0002) and mothers (P = 0.00005). The paternal group showed no significant difference in the genotype distribution of IL-10 between cases and controls.
  
  
  CONCLUSIONS
  In conclusion, IL-10 G-1082A gene promoter polymorphism may act as a major genetic regulator in the etiology of spontaneous abortions.
authors.affiliation: Department of Cell Biology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, India.
authors.forename: M
authors.initials: M
authors.lastname: Vidyadhari
authors.forename: M
authors.initials: M
authors.lastname: Sujatha
authors.forename: P
authors.initials: P
authors.lastname: Krupa
authors.forename: A
authors.initials: A
authors.lastname: Jyothy
authors.forename: Pratibha
authors.initials: P
authors.lastname: Nallari
authors.forename: A
authors.initials: A
authors.lastname: Venkateshwari
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008423:Maternal Age; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011401:Promoter Regions, Genetic
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10