title: The genetic toolbox for Acidovorax temperans.
abstract: Environmental isolates belonging to the genus Acidovorax play a crucial role in degrading a wide range of pollutants. Studies on Acidovorax are currently limited for many species due to the lack of genetic tools. Here, we described the use of the replicon from a small, cryptic plasmid indigenous to Acidovorx temperans strain CB2, to generate stably maintained shuttle vectors. In addition, we have developed a scarless gene knockout technique, as well as establishing green fluorescent protein (GFP) reporter and complementation systems. Taken collectively, these tools will improve genetic manipulations in the genus Acidovorax.
authors.affiliation: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. Electronic address: ssboycheva@gmail.com.
authors.forename: S S
authors.initials: SS
authors.lastname: Boycheva
authors.affiliation: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: F B
authors.initials: FB
authors.lastname: Pichler
authors.affiliation: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: B D
authors.initials: BD
authors.lastname: Heijstra
authors.affiliation: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: K E M
authors.initials: KE
authors.lastname: Lau
authors.affiliation: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: S J
authors.initials: SJ
authors.lastname: Turner
mesh_terms: D042621:Comamonadaceae / Q000235:genetics* / Q000378:metabolism; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012093:Replicon
keywords: Counterselectable marker; Indigenous plasmid; Recombination; Scarless; Shuttle vector; gfp