title: RNA-Seq analysis and whole genome DNA-binding profile of theVibrio choleraehistone-like nucleoid structuring protein (H-NS).
abstract: The data described in this article pertain to the genome-wide transcription profiling of aVibrio choleraemutant lacking the histone-like nucleoid structuring protein (H-NS) and the mapping of the H-NS chromosome binding sites [1, 2]. H-NS is a nucleoid-associated protein with two interrelated functions: organization of the bacterial nucleoid and transcriptional silencing [3]. Both functions require DNA binding and protein oligomerization [4, 5]. H-NS commonly silences the expression of virulence factors acquired by lateral gene transfer [6]. The highly pleiotropic nature ofhnsmutants inV. choleraeindicates that H-NS impacts a broad range of cellular processes such as virulence, stress response, surface attachment, biofilm development, motility and chemotaxis. We used aV. choleraestrain harboring a deletion ofhnsand a strain expressing H-NS tagged at the C-terminus with the FLAG epitope to generate datasets representing thehnstranscriptome and DNA binding profile under laboratory conditions (LB medium, 37°C). The datasets are publicly available at the Gene Expression Omnibus (GEO) repository (http://www.ncbi.nlm.nih.gov/geo/) with accession numbers GSE62785 and GSE64249.
authors.affiliation: University of Alabama at Birmingham, Department of Microbiology, Birmingham, Alabama ; Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, Atlanta, Georgia.
authors.forename: Julio C
authors.initials: JC
authors.lastname: Ayala
authors.affiliation: Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, Atlanta, Georgia.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, Atlanta, Georgia.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Benitez
authors.affiliation: Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, Atlanta, Georgia.
authors.forename: Anisia J
authors.initials: AJ
authors.lastname: Silva