title: Krüppel-like transcription factor 8 (Klf8) is expressed and active in the neurons of the mouse brain.
abstract: Krüppel-like transcription factor 8 (KLF8) is a transcription factor suggested to be involved in various cellular events, including malignant cell transformation, still its expression in the adult rodent brain remained unknown. To analyze Klf8 in the mouse brain and to identify cell types expressing it, a specific transgenic Klf8(Gt1Gaj) mouse was used. The resulting Klf8 gene-driven β-galactosidase activity was visualized by X-gal histochemical staining of the brain sections. The obtained results were complemented by in situ RNA hybridization and immunohistochemistry. Klf8 was highly expressed throughout the adult mouse brain gray matter including the cerebral cortex, hippocampus, olfactory bulb, hypothalamus, pallidum, and striatum, but not in the cerebellum. Immunofluorescent double-labeling revealed that KLF8-immunoreactive cells were neurons, and the staining was located in their nucleus. This was the first study showing that Klf8 was highly expressed in various regions of the mouse brain and in particular in the neurons, where it was localized in the cell nuclei.
authors.affiliation: University of Zagreb School of Medicine, Croatian Institute for Brain Research, Zagreb, Croatia.
authors.forename: Marina
authors.initials: M
authors.lastname: Dobrivojević
authors.affiliation: University of Zagreb School of Medicine, Croatian Institute for Brain Research, Zagreb, Croatia.
authors.forename: Nikola
authors.initials: N
authors.lastname: Habek
authors.affiliation: University of Zagreb School of Medicine, Croatian Institute for Brain Research, Zagreb, Croatia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Kapuralin
authors.affiliation: University of Zagreb School of Medicine, Croatian Institute for Brain Research, Zagreb, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Ćurlin
authors.affiliation: University of Zagreb School of Medicine, Croatian Institute for Brain Research, Zagreb, Croatia. Electronic address: srecko.gajovic@hiim.hr.
authors.forename: Srećko
authors.initials: S
authors.lastname: Gajović
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D015870:Gene Expression; D051741:Kruppel-Like Transcription Factors; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C475812:Klf8 protein, mouse; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors
keywords: Krüppel-like factor 8; Mouse brain; Neurons; Transcription factor