title: Fuzzy clustering demonstrates that codon 72 SNP rs1042522 of TP53 gene associated with HNSCC but not with prognoses.
abstract: It is estimated that 7.6 million people will die as a consequence of head and neck squamous cell carcinoma (HNSCC). Genetic predisposition has emerged as an important risk factor in the development and prognosis of HNSCC. Considering this, the aim of the current study is to assess whether codon 72 SNP of the TP53 gene (rs1042522) is associated with an increased odds ratio of developing HNSCC or with a worse prognosis in patients with HNSCC. Analysis of the rs1042522 in HNSCC patients and in control individuals. Differences between the case and control groups were determined using chi-squared tests. Multivariate analysis was performed to evaluate the odds ratio of HNSCC. Fussy C Means Clustering was to cluster HNSCC patients for survival analyses. Time of survival was calculated using the Kaplan-Meier estimator and comparing this to the log rank test. Statistical significance was set at p < 0.05. A total of 71.4 % of the Arg/Arg genotype were from HNSCC patients, while only 28.6 % of Arg/Arg genotype were found in the control group. Logistic regression demonstrated that the Arg/Arg genotype, smoking, and alcohol consumption increase the odds ratio of HNSCC. No association between TP53 codon 72 polymorphism and P53 expression. No association between rs1042522 and survival or prognoses was observed. This study identified that individuals carrying the arginine allele at rs1042522 have an increased odds ratio of HNSCC. However, no association between codon 72 SNP of the TP53 gene and HNSCC prognosis or P53 expression was observed.
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Ugo Borges
authors.initials: UB
authors.lastname: Pinheiro
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Carlos Alberto de Carvalho
authors.initials: CA
authors.lastname: Fraga
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Danilo Cangussu
authors.initials: DC
authors.lastname: Mendes
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Lucyana Conceição
authors.initials: LC
authors.lastname: Farias
authors.affiliation: Department of Medicine and Department of Computer Science, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Cláudio Marcelo
authors.initials: CM
authors.lastname: Cardoso
authors.affiliation: Department of Medicine and Department of Computer Science, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Christine Mendes
authors.initials: CM
authors.lastname: Silveira
authors.affiliation: Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Marcos Flávio Silveira Vasconcelos
authors.initials: MF
authors.lastname: D'Angelo
authors.affiliation: Center for Research, The Educative Association of Brazil, Montes Claros, Brazil.
authors.forename: Kimberly Marie
authors.initials: KM
authors.lastname: Jones
authors.affiliation: Department Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Sérgio Henrique Souza
authors.initials: SH
authors.lastname: Santos
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
authors.forename: Alfredo Maurício Batista
authors.initials: AM
authors.lastname: de Paula
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil. andreluizguimaraes@gmail.com.
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D003062:Codon; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011379:Prognosis; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D003062:Codon; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Oral cancer; Survival; Tumor suppressor gene; p53