title: Characterization of a Thioredoxin-1 Gene from Taenia solium and Its Encoding Product.
abstract: Taenia solium thioredoxin-1 gene (TsTrx-1) has a length of 771 bp with three exons and two introns. The core promoter gene presents two putative stress transcription factor binding sites, one putative TATA box, and a transcription start site (TSS). TsTrx-1 mRNA is expressed higher in larvae than in adult. This gene encodes a protein of 107 amino acids that presents the Trx active site (CGPC), the classical secondary structure of the thioredoxin fold, and the highest degree of identity with the Echinococcus granulosus Trx. A recombinant TsTrx-1 (rTsTrx-1) was produced in Escherichia coli with redox activity. Optimal activity for rTsTrx-1 was at pH 6.5 in the range of 15 to 25°C. The enzyme conserved activity for 3 h and lost it in 24 h at 37°C. rTsTrx-1 lost 50% activity after 1 h and lost activity completely in 24 h at temperatures higher than 55°C. Best storage temperature for rTsTrx-1 was at -70°C. It was inhibited by high concentrations of H₂O₂ and methylglyoxal (MG), but it was inhibited neither by NaCl nor by anti-rTsTrx-1 rabbit antibodies that strongly recognized a ~12 kDa band in extracts from several parasites. These TsTrx-1 properties open the opportunity to study its role in relationship T. solium-hosts.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Ciudad Universitaria, Edificio A, No. 2 Piso, 04510 Mexico, DF, Mexico.
authors.forename: Lucía
authors.initials: L
authors.lastname: Jiménez
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Ciudad Universitaria, Edificio A, No. 2 Piso, 04510 Mexico, DF, Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Rodríguez-Lima
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Ciudad Universitaria, Edificio A, No. 2 Piso, 04510 Mexico, DF, Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Ochoa-Sánchez
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Ciudad Universitaria, Edificio A, No. 2 Piso, 04510 Mexico, DF, Mexico.
authors.forename: Abraham
authors.initials: A
authors.lastname: Landa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003551:Cysticercosis / Q000235:genetics* / Q000473:pathology / Q000662:veterinary; D006790:Host-Parasite Interactions; D006801:Humans; D017433:Protein Structure, Secondary; D013552:Swine; D041201:Taenia solium / Q000235:genetics* / Q000472:pathogenicity; D013879:Thioredoxins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D013879:Thioredoxins