title: Efficacious long-term cooling and freezing of Sapajus apella semen in ACP-118(®).
abstract: The objectives of the present study were to test the effect of coconut water solution (CWS), TES-TRIS and ACP-118(®) on the seminal cooling and cryopreservation of semen from capuchin monkeys (Sapajus apella). Semen was collected from six males by electro-ejaculation, diluted in TES-TRIS, CWS or ACP-118(®), and maintained at 4°C for 28h. Semen was subsequently evaluated (Experiment I) or cryopreserved in the presence of different glycerol concentrations (3%, 5% or 7%) (Experiment II). ACP-118(®) was the preferred extender to preserve sperm motility and viability after 28h incubation at 4°C. Cooled sperm were successfully frozen-thawed in a medium containing 3% glycerol. After thawing, sperm retained the capacity to fertilize oocytes and zygotes were obtained. In conclusion, ACP-118(®) can be effectively and efficiently used as extender for the cooling of S. apella semen. Furthermore, cryopreservation using ACP-118(®) by adding 3% glycerol is suitable to maintain sperm morphology and the capacity of these cells to fertilize in vitro.
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Belém, Pará, Brazil.
authors.forename: D L
authors.initials: DL
authors.lastname: Leão
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Belém, Pará, Brazil.
authors.forename: S A
authors.initials: SA
authors.lastname: Miranda
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Belém, Pará, Brazil.
authors.forename: A B
authors.initials: AB
authors.lastname: Brito
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Belém, Pará, Brazil.
authors.forename: J S
authors.initials: JS
authors.lastname: Lima
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Belém, Pará, Brazil; Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: R R
authors.initials: RR
authors.lastname: Santos
authors.affiliation: Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Belém, Pará, Brazil; Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil. Electronic address: R.Rodriguesdossantos@pq.cnpq.br.
authors.forename: S F S
authors.initials: SF
authors.lastname: Domingues
mesh_terms: D000818:Animals; D002428:Cebus; D003059:Cocos; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D008297:Male; D012661:Semen / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents
keywords: Capuchin monkey; Coconut; Glycerol; TES–TRIS