title: Grape seed proanthocyanidin extracts prevent high glucose-induced endothelia dysfunction via PKC and NF-κB inhibition.
abstract: In our study, it has been detected in vivo and in vitro that GSPE reversed high glucose-induced the increase of ICAM-1 and VCAM-1. It is shown that by western blotting detection, GSPE significantly inhibited the activation of NF-κB induced by high glucose while there was significant decrease of the expression of PKC with GSPE intervention. By adding the NF-κB blocker PDTC and the PKC inhibitor peptide 19-31(10(-6) M), no significant difference was found in the levels of VCAM-1 and ICAM-1 among GSPE group, the PKC inhibitor peptide 19-31-added GSPE group and the PDTC-added GSPE group. So the conclusion could be drawn that PKC inhibition must be involved in GSPE decreasing the level of ICAM-1 and VCAM-1.We proved for the first time that GSPE prevented high glucose-induced the increase of ICAM-1 and VCAM-1 by PKC and NF-κB inhibition. These findings show a novel mechanism of the action GSPE preventing endothelial dysfunction, which may have clinical application values.
authors.affiliation: a Department of Nutrition and Food Hygiene , School of Public Health, Peking University , Beijing , China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Ding
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Dai
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000633:toxicity; D056604:Grape Seed Extract / Q000008:administration & dosage*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics*; D044945:Proanthocyanidins / Q000008:administration & dosage*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000235:genetics*; D015398:Signal Transduction; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
chemical_list: D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D016328:NF-kappa B; D044945:Proanthocyanidins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D011493:Protein Kinase C; D005947:Glucose
keywords: endothelial dysfunction; grape seed proanthocyanidin extracts; high glucose; intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesionmolecule-1 (VCAM-1); protein kinase C (PKC) and nuclear factor kappaB (NF-κB)