title: Swiprosin-1 stimulates cancer invasion and metastasis by increasing the Rho family of GTPase signaling.
abstract: Ectopic expression of Swiprosin-1, an actin-binding protein (also known as EF hand domain containing 2; EFHD2), enhanced motile protrusions associated with actin, such as lamellipodia and membrane ruffles. Swiprosin-1 levels were increased in various human cancer tissues, particularly at highly invasive stages of malignant melanoma. Expression of Swiprosin-1 was correlated with that of epidermal growth factor receptor (EGFR) and induced by EGF. In a mouse metastasis model, Swiprosin-1 overexpression induced pulmonary metastasis whereas its knockdown led to marked inhibition of metastasis of highly invasive melanoma cells. Swiprosin-1 at the lamellipodia and membrane ruffles controlled the direction of cell protrusion and enhanced migration velocity through activating the Rho family of small GTPases, including Rac1, Cdc42 and RhoA. Our collective findings support the potential utility of Swiprosin-1 as a therapeutic target to prevent cancer invasion and metastasis.
authors.affiliation: Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Yun Hyun
authors.initials: YH
authors.lastname: Huh
authors.affiliation: Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Sena
authors.initials: S
authors.lastname: Oh
authors.affiliation: Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Yu Ra
authors.initials: YR
authors.lastname: Yeo
authors.affiliation: Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: In Hee
authors.initials: IH
authors.lastname: Chae
authors.affiliation: Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: So Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Pathology, Chonnam National University Hwasun Hospital, Hwasun, Korea.
authors.forename: Ji Shin
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Dermatology, Chonnam National University Hospital, Gwangju, Korea.
authors.forename: Sook Jung
authors.initials: SJ
authors.lastname: Yun
authors.affiliation: The Division of Natural Medical Sciences, College of Health Science, Chosun University, Gwangju, Korea.
authors.forename: Kyu Yeong
authors.initials: KY
authors.lastname: Choi
authors.affiliation: Dental Science Research Institute and Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Korea.
authors.forename: Je-Hwang
authors.initials: JH
authors.lastname: Ryu
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Chang-Duk
authors.initials: CD
authors.lastname: Jun
authors.affiliation: Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Woo Keun
authors.initials: WK
authors.lastname: Song
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D045325:HCT116 Cells; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D051379:Mice; D015398:Signal Transduction; D014162:Transfection; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; C502299:EFHD2 protein, human; C530752:EFHD2 protein, mouse; D020742:rhoA GTP-Binding Protein
keywords: Rho GTPase; Swiprosin-1; invasion; metastasis; migration