title: Proteins involved in focal adhesion signaling pathways are differentially regulated in experimental branch retinal vein occlusion.
abstract: Branch retinal vein occlusion (BRVO) is a common retinal vascular disease, but global protein changes following the condition remain largely unelucidated. To bring new insights into pathological processes and identify potential therapeutic targets, large-scale retinal protein changes following BRVO were studied by combining a porcine model of experimental BRVO with proteomic analysis by label-free liquid chromatography mass spectrometry. Among a total set of 1974 proteins, 52 significantly upregulated proteins and 10 significantly downregulated proteins were identified in retinas with BRVO after 15 days. Significantly upregulated proteins were involved in signaling pathways of focal adhesion via integrin and blood coagulation. Proteins involved in focal adhesion signaling included collagen α-2 chain, laminin subunit β-2, laminin subunit γ-1, lipocalin-7, nidogen-2, osteopontin, integrin-β, α-actinin-1, isoform 2 of α-actinin-1, talin-2 and filamin C. The identified proteins indicate that BRVO was associated with extracellular matrix remodeling processes. The present study identified focal adhesion signaling and ECM remodeling as important biological mechanisms to evaluate in the search for signaling pathways that promote neovascularisation and macular edema following BRVO.
authors.affiliation: Department of Ophthalmology, Aalborg University Hospital, Aalborg, Denmark; Biomedical Research Laboratory, Aalborg University Hospital, Aalborg, Denmark. Electronic address: L.cehofski@rn.dk.
authors.forename: Lasse Jørgensen
authors.initials: LJ
authors.lastname: Cehofski
authors.affiliation: Department of Ophthalmology, Aalborg University Hospital, Aalborg, Denmark.
authors.forename: Anders
authors.initials: A
authors.lastname: Kruse
authors.affiliation: Biomedical Research Laboratory, Aalborg University Hospital, Aalborg, Denmark.
authors.forename: Benedict
authors.initials: B
authors.lastname: Kjærgaard
authors.affiliation: Department of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Allan
authors.initials: A
authors.lastname: Stensballe
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus, Denmark.
authors.forename: Bent
authors.initials: B
authors.lastname: Honoré
authors.affiliation: Department of Ophthalmology, Aalborg University Hospital, Aalborg, Denmark; Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Vorum
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000235:genetics*; D022001:Focal Adhesions / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D063990:Gene Ontology; D040901:Proteomics / Q000379:methods; D012170:Retinal Vein Occlusion / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D005136:Eye Proteins
keywords: Adhesion; Biological marker; Branch retinal vein occlusion; Extracellular matrix; Integrin; Lipocalin; Mass spectrometry; Myosin 9; Osteopontin; Proteomics; Retina