title: Downregulation of microRNA-382 is associated with poor outcome of esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  To study the potential prognostic role of microRNA-382 (miR-382) in esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  Forty six patients were divided into 2 groups according to postoperative survival time: the poor outcome group (28 patients), who showed early metastasis but no recurrence, and died within 1 year after surgery, 12 patients of the group received postoperative chemotherapy treatment that was given after early metastasis happening; the good outcome group (18 patients), who had no clinical metastasis and recurrence, and survived 5 years or more after surgery, all patients did not receive any postoperative treatment. Total RNA was extracted from the patients' formalin-fixed and paraffin-embedded esophageal cancer tissues. miR-382 level was evaluated using high-throughput real-time quantitative polymerase chain reaction analysis. The correlation between miR-382 level and clinicopathologic features was analyzed through COX regression model, and Kaplan-Meier analysis was used to analyze the relationship between miR-382 level and patient survival time.
  
  
  RESULTS
  miR-382 was differentially expressed in the two groups. Overall the average miR-382 level in the ESCC patients with good outcome was 9.8 ± 3.8, while miR-382 level in the ESCC patients with poor outcome was 3.0 ± 0.8. The differences of miR-382 levels between two groups were significant (P < 0.05). Kaplan-Meier analysis results showed that miR-382 expression level generally had a significant reverse-correlation with ESCC patient survival time (P < 0.001), in which the patients with higher expressions of miR-382 had a longer survival time either among individuals with the same tumor stage or among the overall patients.
  
  
  CONCLUSIONS
  miR-382 levels are reverse-correlated with ESCC poor outcomes, suggesting that miR-382 could be a potential predictive biomarker for both prognosis and treatment of ESCC.
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qi
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Lu
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Yao
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Ting-Min
authors.initials: TM
authors.lastname: Chang
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Xiu-Guang
authors.initials: XG
authors.lastname: Qin
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Ji
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Shang-Guo
authors.initials: SG
authors.lastname: Liu
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Han-Chen
authors.initials: HC
authors.lastname: Li
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Bo Qi, Jian-Guo Lu, Wen-Jian Yao, Xiu-Guang Qin, Shang-Guo Liu, Han-Chen Li, Yu-Zhen Liu, Bao-Sheng Zhao, Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Zhao
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000628:therapy; D017024:Chemotherapy, Adjuvant; D018450:Disease Progression; D018572:Disease-Free Survival; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D000077277:Esophageal Squamous Cell Carcinoma; D016629:Esophagectomy; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C555413:MIRN382 microRNA, human; D035683:MicroRNAs
keywords: Esophageal squamous cell carcinoma; Metastasis; Outcome; Prognosis; miR-382