title: Relative expression analysis of IL-5 and IL-6 genes in tropical sheep breed Pelibuey infected with Haemonchus contortus.
abstract: Haemonchus contortus is a parasitic nematode of Pelibuey sheep, a meat breed used in tropical regions. Due to anthelmintic problems, the identification of hosts resistant to H. contortus is another option of control. The aim of this study was to analyse the relative expression of IL-5 and IL-6 genes in Pelibuey sheep after H. contortus infection. Nineteen lambs infected with H. contortus and three more lambs without infection were studied. The haemonchosis was determined by the number of eggs per gram of faeces (epg) and by the estimation of the percentage of the packed cell volume (%pcv). Peripheral blood mononuclear cells (PBMCs) were obtained to extract RNA at 0, 1, 2, 7, 14, 21 and 28 days after infection to quantify the relative expression of IL-5, IL-6 and GAPDH by real-time PCR. Five lambs were classified as low responders (lr) to haemonchosis with averages of 1519 ± 315·3 epg and 31·49 ± 5·13%pcv, and 14 lambs were identified as high responders (hr) with averages of 530 ± 132 epg and 34·88 ± 3·75%pcv. The expression ratio of IL-5 was significantly different compared with control lambs at 2, 7 and 14 days post-infection (PI), and IL-6 was significantly different after 14 days. The highest level of relative expression for IL-5 and IL-6 genes was 9·9-fold and 12-fold after 2 and 14 days for hr hosts (P < 0·05) compared with control group, respectively. In conclusion, the Pelibuey breed in grazing areas exhibited different expression of IL-5 and IL-6 obtained from PBMCs against H. contortus, suggesting the importance of these cytokines in regulating the nematode infection.
authors.affiliation: Universidad Politécnica del Estado de Morelos, Jiutepec, Morelos, México.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Estrada-Reyes
authors.affiliation: Instituto Nacional de Rehabilitación, México City, Mexico.
authors.forename: A G
authors.initials: AG
authors.lastname: López-Reyes
authors.affiliation: Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Lagunas-Martínez
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Jiutepec, Morelos, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Ramírez-Vargas
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Jiutepec, Morelos, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Olazarán-Jenkins
authors.affiliation: Universidad Politécnica del Estado de Morelos, Jiutepec, Morelos, México.
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Romano
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Jiutepec, Morelos, Mexico.
authors.forename: P
authors.initials: P
authors.lastname: Mendoza-de-Gives
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Jiutepec, Morelos, Mexico.
authors.forename: M E
authors.initials: ME
authors.lastname: López-Arellano
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology; D006188:Haemonchiasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000502:physiology*; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology*; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008800:Mexico; D010270:Parasite Egg Count; D060888:Real-Time Polymerase Chain Reaction; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology
chemical_list: D015848:Interleukin-5; D015850:Interleukin-6
keywords: Haemonchus contortus; IL-5; IL-6; PBMCs; Pelibuey breed; sheep