title: [Study on Genetic Diversity of Twelve Natural Zanthoxylum dissitum Populations].
abstract: OBJECTIVE
  The genetic diversity of twelve natural Zanthoxylum dissitum populations, which is a species of Chinese herbal medicines to four provinces of southwest China, has been investigated.
  
  
  METHODS
  By inter-simple sequence repeat markers (ISSR), the eight primers, which could amplify stable, clear and highly polymorphic bands, were screened from 100 candidate primers.
  
  
  RESULTS
  150 total ISSR discernible bands and 147 polymorphic were amplified by the eight checked primers. On one hand, the percentage of polymorphic bands was 98.0%, on the other hand, the population level the percent of polymorphic bands ranged from 26.0% to 62.0%. The Shannon's information index within species (Hsp) was 0.4175, while the values within population (Hpop) were ranged from 0.1328 to 0.3267. Analysis of molecular variance (ANOVA) revealed that the population genetic variation accounted for 47.98% but the intraspecific variation for 52.02%.
  
  
  CONCLUSIONS
  The high level of genetic diversity exists not only in population but also in species. A high degree of genetic differentiation populations is approved to exist in Zanthoxylum dissitum. These results lay a theoretical foundation for genetic diversity analysis of Zanthoxylum dissitum.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Ji-kang
authors.initials: JK
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Ming-liang
authors.initials: ML
authors.lastname: Fe
mesh_terms: D002681:China; D017931:DNA Primers; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats; D010802:Phylogeny; D032106:Zanthoxylum / Q000235:genetics*
chemical_list: D017931:DNA Primers