title: Bioactivities and extraction optimization of crude polysaccharides from the fruits and leaves of Rubus chingii Hu.
abstract: Polysaccharides of Rubus chingii Hu fruit and leaf were extracted to compare their antioxidant, anti-inflammatory, and anticancer activities against breast cancer cells MCF-7 and liver cancer cells Bel-7402. Results showed that all the tested bioactivities of polysaccharides from leaf (L-Ps) were better than those of polysaccharides from fruit (F-Ps). Response surface methodology was then used to optimize the extraction conditions of polysaccharides from leaf. Additionally, polysaccharides from fruit and leaf were characterized and their contents of total sugars, proteins and uronic acid were compared. It was found that polysaccharides from fruit and leaf were similar in IR and UV absorption, but significantly different in contents of total sugars, protein and uronic acid. Their elution profiles of DEAE-Sepharose fast flow column were different too. The main peak of polysaccharides from fruit was eluted with 0.3 mol/l NaCl solution and the main peak of polysaccharides from leaf was eluted with deionized water. The differences between the two polysaccharides may be responsible for their differences in bioactivities. Further studies are required to explore their complete structural characteristics, structure-activity relationship and the mechanism of their activities.
authors.affiliation: College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Chuan-Li
authors.initials: CL
authors.lastname: Lu
authors.affiliation: College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China. Electronic address: jgjiang@scut.edu.cn.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Jiang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China. Electronic address: zhuwei9201@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D065856:Rubus / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D018836:Inflammation Mediators; D011134:Polysaccharides; D012333:RNA, Messenger
keywords: Bioactivity analyses; Extraction; Polysaccharides; Rubus chingii Hu