title: Effect of Nitric Oxide Synthesis Blockade on the Morphology of Langerhans Islets in August and Wistar Rats with Acute Alloxan Diabetes.
abstract: Alloxan diabetes was modeled in August rats with high activity of the NO system and in Wistar rats, and the effects of NO system blockade (by a course treatment with L-NNA) on Langerhans islet β cells were studied in 15 days. The toxic effects of diabetes on the rat β cells and islets were similar: the content of active β cells in the islets decreased to 15-20%, the number of islets to 24-29% of control. A course of L-NNA reduced the β cell and islet death, in August cells greater than in Wistar: the number of islets in August rats was restored to 81%, in Wistar rats to 60% of initial level; the activity of β cells remained at the control level in the former and 2-fold lower than in the control in the latter. It seems that a less pronounced protective effect of L-NNA in Wistar rats was explained by excessive reduction of NO level essential for β cell regeneration.
authors.affiliation: Research Institute of Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Smirnova
authors.forename: A B
authors.initials: AB
authors.lastname: Michunskaya
authors.forename: O L
authors.initials: OL
authors.lastname: Terekhina
authors.forename: L P
authors.initials: LP
authors.lastname: Kobozeva
authors.forename: S V
authors.initials: SV
authors.lastname: Kruglov
authors.forename: L M
authors.initials: LM
authors.lastname: Belkina
authors.forename: O M
authors.initials: OM
authors.lastname: Pozdnyakov
mesh_terms: D000818:Animals; D001837:Body Weights and Measures; D002452:Cell Count; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects; D007515:Islets of Langerhans / Q000254:growth & development / Q000473:pathology*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology*; D059606:Polydipsia / Q000517:prevention & control; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
chemical_list: D019335:Nitroarginine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase