title: A High-Salt Diet Differentially Modulates Mechanical Activity of Afferent and Efferent Collecting Lymphatics in Murine Iliac Lymph Nodes.
abstract: BACKGROUND
  The lymphatic system contributes to fluid homeostasis in various tissues. Recent evidence suggests that lymphangiogenesis induced by a high-salt diet (HSD) is associated with blood pressure regulation. Lymph nodes, located along lymphatic pathways, are not only important secondary lymphoid tissues for cancer metastasis, inflammation, and immune responses, but are also important for fluid homeostasis. Afferent lymphatics collect lymph from the pre-nodal area and efferent lymphatics drain lymph out of the lymph nodes. However, the difference in mechanical activity between afferent and efferent lymphatics and the effect of a HSD on these vessels have not been shown.
  
  
  RESULTS
  Changes in mechanical activity of isolated afferent and efferent lymphatics in normal salt diet (NSD) and 4-week HSD mice in response to increases in intraluminal pressures from 3 to 7 cmH2O were measured using video-microscopy. The higher intramural pressure equivalently decreased pumping activity of afferent and efferent lymphatics in NSD mice. A HSD suppressed the amplitude, ejection fraction, and stroke volume of afferent lymphatics, leading to marked reductions in pumping activity. In contrast, the pumping activities of efferent lymphatics were resistant to a HSD and were preserved by enhancing the contraction frequency.
  
  
  CONCLUSIONS
  A HSD differentially modulated the mechanical activity of afferent and efferent collecting lymphatics in murine iliac lymph nodes.
authors.affiliation: 1 Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo, Japan .|2 Department of Molecular Vascular Endocrinology, Graduate School of Medicine, The University of Tokyo , Tokyo, Japan .
authors.forename: Risuke
authors.initials: R
authors.lastname: Mizuno
authors.affiliation: 3 Department of Endocrinology and Diabetes, School of Medicine, Saitama Medical University, Saitama, Japan .
authors.forename: Masashi
authors.initials: M
authors.lastname: Isshiki
authors.affiliation: 4 Department of Electronics and Control Engineering, Nagano National College of Technology , Nagano, Japan .
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Ono
authors.affiliation: 5 Division of Clinical Epigenetics, Research Center for Advanced Science and Technology, The University of Tokyo , Tokyo, Japan .
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Nishimoto
authors.affiliation: 4 Department of Electronics and Control Engineering, Nagano National College of Technology , Nagano, Japan .
authors.forename: Toshiro
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004032:Diet; D006339:Heart Rate; D008198:Lymph Nodes / Q000502:physiology*; D008208:Lymphatic System / Q000502:physiology; D042601:Lymphatic Vessels / Q000502:physiology*; D008297:Male; D051379:Mice; D023421:Models, Animal; D017673:Sodium Chloride, Dietary
chemical_list: D017673:Sodium Chloride, Dietary