title: High-resolution, label-free imaging of living cells with direct electron-beam-excitation-assisted optical microscopy.
abstract: High spatial resolution microscope is desired for deep understanding of cellular functions, in order to develop medical technologies. We demonstrate high-resolution imaging of un-labelled organelles in living cells, in which live cells on a 50 nm thick silicon nitride membrane are imaged by autofluorescence excited with a focused electron beam through the membrane. Electron beam excitation enables ultrahigh spatial resolution imaging of organelles, such as mitochondria, nuclei, and various granules. Since the autofluorescence spectra represent molecular species, this microscopy allows fast and detailed investigations of cellular status in living cells.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nawa
authors.forename: Wataru
authors.initials: W
authors.lastname: Inami
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawata
authors.forename: Susumu
authors.initials: S
authors.lastname: Terakawa
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002470:Cell Survival; D004583:Electrons; D021621:Imaging, Three-Dimensional; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure; D055620:Optical Phenomena; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D013194:Staining and Labeling