title: Overexpression of Arabidopsis NADPH-dependent thioredoxin reductase C (AtNTRC) confers freezing and cold shock tolerance to plants.
abstract: Overexpression of AtNTRC (AtNTRC(OE)) in Arabidopsis thaliana led to a freezing and cold stress tolerance, whereas a knockout mutant (atntrc) showed a stress-sensitive phenotype. Biochemical analyses showed that the recombinant AtNTRC proteins exhibited a cryoprotective activity for malate dehydrogenase and lactic dehydrogenase. Furthermore, conclusive evidence of its interaction with nucleic acids in vitro is provided here on the basis of gel shift and electron microscopy analysis. Recombinant AtNTRC efficiently protected RNA and DNA from RNase A and metal catalyzed oxidation damage, respectively. The C-terminal thioredoxin domain is required for the nucleic acid-protein complex formation. From these results, it can be hypothesized that AtNTRC, which is known to be an electron donor of peroxiredoxin, contributes the stability of macromolecules under cold stress.
authors.affiliation: National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon-gun 325-813, Republic of Korea.
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Moon
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Lee
authors.affiliation: National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon-gun 325-813, Republic of Korea.
authors.forename: Su Young
authors.initials: SY
authors.lastname: Shin
authors.affiliation: Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Ho Byoung
authors.initials: HB
authors.lastname: Chae
authors.affiliation: Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Jung
authors.affiliation: Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
authors.affiliation: National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon-gun 325-813, Republic of Korea; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, United States. Electronic address: leejr73@nie.re.kr.
authors.forename: Jung Ro
authors.initials: JR
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21+ program), PMBBRC, Gyeongsang National University, Jinju, Republic of Korea. Electronic address: sylee@gnu.ac.kr.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D005615:Freezing; D009249:NADP / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*
chemical_list: D009696:Nucleic Acids; D009249:NADP; D013880:Thioredoxin-Disulfide Reductase
keywords: Cryoprotective activity; DNA binding; NADPH-dependent thioredoxin reductase; Peroxiredoxin; Thioredoxin