title: DNA methylation is necessary for erythropoietin to improve spatial learning and memory in SAMP8 mice.
abstract: OBJECTIVE
  To reveal the role of Dnmts in the improvement of spatial learning and memory induced by erythropoietin (EPO) in SAMP8 mice.
  
  
  METHODS
  The Morris water maze (MWM) was used to assess spatial learning and memory. Mice were administered by intraperitoneal (i.p.) injection of recombinant human EPO and hippocamppi infusion (IH) of 5-aza-2'-deoxycytidine (5-AZA). The expression of genes Dnmt1, Dnmt3a and Dnmt3b in the hippocampus was detected by real-time qPCR. The level of proteins DNMT1, DNMT3A and DNMT3B was measured by Western blotting.
  
  
  RESULTS
  Spatial learning and memory in SAMP8 were promoted after i.p. injection of EPO (5000IU/kg/day) and expression of Dnmt3b mRNA and DNMT3B proteins in the hippocampus increased. The improved memory by EPO was blocked after IH 5-AZA.
  
  
  CONCLUSIONS
  DNA methylation is necessary for EPO to enhance spatial learning and memory in SAMP8 mice.
authors.affiliation: Department of Neurology, Sichuan Provincial People's Hospital, 610072, 32 West Second Section First Ring Road, Chengdu, Sichuan, China. Electronic address: 18981838652@126.com.
authors.forename: Nengwei
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Neurology, Sichuan Provincial People's Hospital, 610072, 32 West Second Section First Ring Road, Chengdu, Sichuan, China. Electronic address: liujiesyy@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurology, Sichuan Provincial People's Hospital, 610072, 32 West Second Section First Ring Road, Chengdu, Sichuan, China. Electronic address: xiaogang817@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Yi
authors.affiliation: Department of Neurology, Sichuan Provincial People's Hospital, 610072, 32 West Second Section First Ring Road, Chengdu, Sichuan, China. Electronic address: 18981838651@126.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Neurology, Sichuan Provincial People's Hospital, 610072, 32 West Second Section First Ring Road, Chengdu, Sichuan, China. Electronic address: xiaojun3@medmail.com.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Neurology, Sichuan Provincial People's Hospital, 610072, 32 West Second Section First Ring Road, Chengdu, Sichuan, China. Electronic address: guofuqiang2005@126.com.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Guo
mesh_terms: D000818:Animals; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D019175:DNA Methylation / Q000187:drug effects / Q000502:physiology; D000068817:Epoetin Alfa / Q000494:pharmacology*; D006397:Hematinics / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology; D051379:Mice; D065853:Spatial Learning / Q000187:drug effects / Q000502:physiology; D016896:Treatment Outcome; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000068817:Epoetin Alfa; D006397:Hematinics; D000097584:DNA Methyltransferase 3B
keywords: DNA methylation; Erythropoietin; Learning and memory