title: Nickel-Iron Hydrogenases: High-Resolution Crystallography Resolves the Hydride, but Not the Debate.
abstract: An (X-ray) eye for detail: Modern high-resolution protein crystallography allows H atoms to be located. Applied to nickel-iron hydrogenase, X-ray structural analysis has finally confirmed the presence of an active-site hydride and thiol, as well as unveiling the intricate pathways that protons take to and from the active site.
authors.affiliation: IBS Center for Multidimensional Carbon Materials, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Eonyan-eup, Ulju-gun, Ulsan 689-798 (South Korea). schilter@ibs.re.kr.
authors.forename: David
authors.initials: D
authors.lastname: Schilter
mesh_terms: D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D006859:Hydrogen / Q000032:analysis* / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry*
chemical_list: D006859:Hydrogen; D006864:Hydrogenase
keywords: X-ray crystallography; biomimetic models; enzyme catalysis; hydrides; hydrogenases