title: A MoS₂-based system for efficient immobilization of hemoglobin and biosensing applications.
abstract: A novel hydrogen peroxide (H2O2) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2) nanocomposite film modified glass carbon electrode. The AuNPs@MoS2 nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H2O2 and NO. Under optimal conditions, the current response was linear with the concentration of H2O2 and NO in the range from 10 to 300 μM and 10 to 1100 μM with a detection limit of 4 and 5 μM, respectively. This MoS2-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS2 nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.forename: Min
authors.initials: M
authors.lastname: Zou
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Haofan
authors.initials: H
authors.lastname: Sun
authors.forename: Dun
authors.initials: D
authors.lastname: Pan
authors.forename: Hao
authors.initials: H
authors.lastname: Pei
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.forename: Lihui
authors.initials: L
authors.lastname: Yuwen
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide; D055767:Immobilized Proteins / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D009569:Nitric Oxide
chemical_list: D004220:Disulfides; D006454:Hemoglobins; D055767:Immobilized Proteins; D009569:Nitric Oxide; D006046:Gold; D008982:Molybdenum; D006861:Hydrogen Peroxide; C082964:molybdenum disulfide