title: Anti-Inflammatory Effect of Angelica gigas via Heme Oxygenase (HO)-1 Expression.
abstract: Angelica gigas (AG) is effective against various medical conditions such as bacterial infection, inflammation, and cancer. It contains a number of coumarin compounds and the group of interest is the pyranocoumarin, which comprises decursin and decursinol angelate. This group has an effect on controlling inflammation, which is caused by excessive nitric oxide (NO) production. Heme oxygenases (HOs), particularly HO-1, play a role in regulating the production of NO. Thus, this study aimed to investigate the anti-inflammatory effects of AG by measuring HO-1 expression. Treatments with CH2Cl2 layer and Angelica gigas extract (AGE) showed the highest NO inhibition effects. Decursin, decursinol angelate, and nodakenin were isolated from the CH2Cl2 layer of AGE. Decursin also demonstrated the highest anti-oxidative effect among the coumarins. Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin. This is owing to the combination effect of the coumarins present within AGE, which is a solvent extract of AG. The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG. Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.
authors.affiliation: Department of Biological and Environmental Science, Dongguk University, Goyang 410-820, Korea. jhcho@dongguk.edu.
authors.forename: Joon Hyeong
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Biological Science, Gachon University, Seongnam 461-701, Korea. jjung@nmr.kr.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Kwon
authors.affiliation: Department of Biological Science, Gachon University, Seongnam 461-701, Korea. ym473@nmr.kr.
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Cho
authors.affiliation: Department of Biological Science, Gachon University, Seongnam 461-701, Korea. ihyekim@gmail.com.
authors.forename: Inhye
authors.initials: I
authors.lastname: Kim
authors.affiliation: Department of Biological Science, Gachon University, Seongnam 461-701, Korea. sckang73@gachon.ac.kr.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001578:Benzopyrans / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D003374:Coumarins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D005960:Glucosides / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001578:Benzopyrans; D002087:Butyrates; D003374:Coumarins; D005960:Glucosides; D008565:Membrane Proteins; D010936:Plant Extracts; D009569:Nitric Oxide; C101278:decursin; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; C471579:nodakenin
keywords: Angelica gigas; Anti-inflammation; HO-1 expression; coumarins; decursin; vascular smooth muscle cells