title: Amine-functionalized TiO₂ nanoparticles for highly selective enrichment of phosphopeptides.
abstract: Specific enrichment of trace phosphoproteins or phosphopeptides from complex biological samples prior to mass spectrometry analysis is of profound significance for in-depth phosphoproteomics. In this work, an amine-functionalized TiO2 nano-material with N'[3-(trimethoxysilyl)-propyl] diethylenetriamine (TPDA) modified on the surface of nanoparticle TiO2 (TiO2@TPDA) was successfully designed and applied for the enrichment of phosphopeptides. Compared with pure TiO2, the novel prepared TiO2@TPDA possessed lower Lewis acidity, enhanced hydrophilicity and stronger affinity to phosphopeptides, and its performance for selective and effective enrichment of phosphopeptide was investigated by the standard protein digests and human serum.
authors.affiliation: College of Life Science, Nanjing Normal University, No.1, WenYuan Road, QiXia District, Nanjing, Jiangsu Province 210023, China; Proteome Experiment Center, Nanjing Normal University, Nanjing, Jiangsu Province 210023, China. Electronic address: lhl7083609@163.com.
authors.forename: Hailong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Analysis and Testing Center, Nanjing Normal University, Nanjing, Jiangsu Province 210023, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Analysis and Testing Center, Nanjing Normal University, Nanjing, Jiangsu Province 210023, China.
authors.forename: Heyong
authors.initials: H
authors.lastname: Huang
mesh_terms: D002364:Caseins / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D002364:Caseins; C575344:N-(3-trimethoxysilylpropyl)diethylenetriamine; D017646:Organosilicon Compounds; D010748:Phosphopeptides; D011073:Polyamines; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium
keywords: Amine-functionalized TiO(2); Enrichment; Mass spectrometry analysis; Phosphopeptides