title: Decreased expression of long non-coding RNA NBAT-1 is associated with poor prognosis in patients with clear cell renal cell carcinoma.
abstract: OBJECTIVE
  accumulating evidence suggest that long non-coding RNAs (lncRNAs) may play important roles in human cancers. LncRNA neuroblastoma associated transcript-1 (NBAT-1) was initially identified to be involved in the progression of neuroblastoma. However, there is no report about the role of NBAT-1 in clear cell renal cell carcinoma (ccRCC). The purpose of this study is to investigate the clinical significant of NBAT-1 in ccRCC.
  
  
  METHODS
  the expression pattern of NBAT-1 in ccRCC patients and renal cancer cell lines was detected by using quantitative real-time PCR (qRT-PCR), and its correlation with clinicopathologic features and prognosis of patients with ccRCC was assessed by Kaplan-Meier method and Cox proportional hazards model, respectively. Small interfering RNA (siRNA) was transfected into 786-O and ACHN cells to determine the effect of NBAT-1 knockdown on renal cancer cells.
  
  
  RESULTS
  NBAT-1 expression is significantly decreased in ccRCC tissues and renal cancer cells compared with adjacent normal tissues and normal human proximal tubule epithelial cell line HK-2, and its low level is associated with advanced features and poor prognosis. Also, multivariate analysis identified NBAT-1 expression as an independent prognostic factor for ccRCC. In vitro assays indicated that knockdown of NBAT-1 expression increased renal cancer cell proliferation, migration and invasion.
  
  
  CONCLUSIONS
  NBAT-1 is a novel molecular correlated with ccRCC progression; and it may represent a prognostic biomarker and therapeutic target in renal cancer diagnosis and treatment.
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Nanjing Medical University Nanjing, China ; Department of Urology, The First Affiliated Hospital of Bengbu Medical College Bengbu Anhui, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xue
authors.affiliation: Department of Urology, The First Affiliated Hospital of Bengbu Medical College Bengbu Anhui, China.
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Li
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Che
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
authors.forename: Feng-Qiang
authors.initials: FQ
authors.lastname: Yang
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Nanjing Medical University Nanjing, China ; Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai, China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Zheng
mesh_terms: D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D015996:Survival Rate
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering
keywords: NBAT-1; clear cell renal cell carcinoma; long non-coding RNAs; prognosis