title: Evaluating the Thickness of Multivalent Glycopolymer Brushes for Lectin Binding.
abstract: Electrochemical impedance spectroscopy (EIS) is applied for investigating binding of lectins to multivalent glycopolymer brushes grafted from interdigital gold microelectrodes. By variation of the measuring frequency, EIS allows simultaneous analysis of binding at different subnanometer distances from the sensor surfaces. In this way, the binding dynamics along the brushes are quantified, giving an idea about the motion of the lectin through the brush layer. Two different brush lengths are investigated, revealing distinct dynamics of lectin binding due to changing topology of the brushes. Moreover, very low K D values in the nanomolar range are obtained. This unique platform may be used as sophisticated biosensor for detailed investigation of high-affinity protein binding to poly-mer layers.
authors.affiliation: Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Sommerfeldstr. 24, 52074, Aachen, Germany.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Lazar
authors.affiliation: DWI-Leibniz Institut für Interaktive Materialien e.V, Lehrstuhl für Makromolekulare Materialien und Oberflächen, Forckenbeckstr., 50, 52074, Aachen, Germany.
authors.forename: Hyunji
authors.initials: H
authors.lastname: Park
authors.affiliation: Laboratory for Biomaterials Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074, Aachen, Germany.
authors.forename: Ruben R
authors.initials: RR
authors.lastname: Rosencrantz
authors.affiliation: DWI-Leibniz Institut für Interaktive Materialien e.V, Lehrstuhl für Makromolekulare Materialien und Oberflächen, Forckenbeckstr., 50, 52074, Aachen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Böker
authors.affiliation: Laboratory for Biomaterials Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074, Aachen, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Elling
authors.affiliation: Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Sommerfeldstr. 24, 52074, Aachen, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Schnakenberg
mesh_terms: D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D008839:Microelectrodes; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D037102:Lectins; D011108:Polymers; D006046:Gold
keywords: atom transfer radical polymerization (ATRP); electrochemical impedance spectroscopy (EIS); glycopolymers; lectins; microfluidics