title: Variable Results for Uveal Melanoma-Specific Gene Expression Profile Prognostic Test in Choroidal Metastasis.
abstract: OBJECTIVE
  We report our experience with uveal melanoma-specific gene expression profile (GEP) testing on a series of choroidal metastatic tumors confirmed by cytopathology so that clinicians may be aware that receiving a class 1 or class 2 test result in nonmelanoma is possible.
  
  
  METHODS
  Retrospective review of all cytopathology and DecisionDx-UM GEP reports between January 2012 to December 2014 from intraoperative fine-needle aspiration biopsy of choroidal tumors undergoing brachytherapy. Four patients were identified to have cytopathology consistent with a nonmelanoma primary. All 4 patients presented with a unilateral, single choroidal tumor, which was treated with iodine-125 brachytherapy and underwent intraoperative fine-needle aspiration biopsy for cytopathology and uveal melanoma-specific GEP testing for molecular prognostication. Gene expression profile testing of the choroidal tumor in each patient revealed class 1A in 3 patients and class 2 in 1 patient.
  
  
  CONCLUSIONS
  DecisionDx-UM GEP may be a helpful test for molecular prognostication in patients with uveal melanoma; however, class 1 and class 2 test results are indeed possible in the setting of a nonmelanoma malignancy. We recommend that cytopathology and/or other melanoma-specific testing be performed in all cases of suspected choroidal melanoma because GEP with this assay is unable to rule out the diagnosis of a choroidal melanoma.
authors.affiliation: Department of Ophthalmology, Stein Eye Institute, University of California, Los Angeles.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Klufas
authors.affiliation: Department of Ophthalmology, Stein Eye Institute, University of California, Los Angeles.
authors.forename: Sujit
authors.initials: S
authors.lastname: Itty
authors.affiliation: Department of Ophthalmology, Stein Eye Institute, University of California, Los Angeles.
authors.forename: Colin A
authors.initials: CA
authors.lastname: McCannel
authors.affiliation: Department of Ophthalmology, Stein Eye Institute, University of California, Los Angeles.
authors.forename: Ben J
authors.initials: BJ
authors.lastname: Glasgow
authors.affiliation: Department of Ophthalmology, Stein Eye Institute, University of California, Los Angeles.
authors.forename: Christian
authors.initials: C
authors.lastname: Moreno
authors.affiliation: Department of Ophthalmology, Stein Eye Institute, University of California, Los Angeles.
authors.forename: Tara A
authors.initials: TA
authors.lastname: McCannel
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D001918:Brachytherapy; D002830:Choroid Neoplasms / Q000235:genetics* / Q000532:radiotherapy / Q000556:secondary; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007457:Iodine Radioisotopes / Q000627:therapeutic use; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000532:radiotherapy / Q000556:secondary; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis; D012189:Retrospective Studies; D014792:Visual Acuity
chemical_list: D007457:Iodine Radioisotopes; D009363:Neoplasm Proteins