title: A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes.
abstract: 6α-Hydroxylup-20(29)-en-3-on-28-oic acid (1), a natural triterpenoid, was found to possess the ability in a dose-dependent manner inhibiting hormone-induced adipocyte differentiation in 3T3-L1 preadipocytes, and restoring glucose consuming ability in dexamethasone (DXM)-induced insulin resistant 3T3-L1 adipocytes. Compound 1 was also found to ameliorate DXM-induced adipocyte dysfunction in lipolysis and adipokine secretion. Mechanistic studies revealed that 1 inhibited adipocyte differentiation in 3T3-L1 preadipocytes via down-regulating hormone-stimulated gene transcription of peroxisome proliferator-activated receptor γ and CCAAT-enhancer-binding protein alpha which are key factors in lipogenesis, and restored DXM-impaired glucose consuming ability in differentiated 3T3-L1 adipocytes via repairing insulin signaling pathway and activating down-stream signaling transduction by phosphorylation of signaling molecules PI3K/p85, Akt2 and AS160, thus leading to increased translocation of glucose transporter type 4 and transportation of glucose.
authors.affiliation: Laboratory of Chinese Herbal Drug Discovery, Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou, 510405, People's Republic of China.
authors.forename: Ji-Huan
authors.initials: JH
authors.lastname: Qin
authors.forename: Jun-Zeng
authors.initials: JZ
authors.lastname: Ma
authors.forename: Xing-Wei
authors.initials: XW
authors.lastname: Yang
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Hu
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Lin-Chun
authors.initials: LC
authors.lastname: Fu
authors.forename: Ru-Hua
authors.initials: RH
authors.lastname: Tian
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Shen