title: A doubly responsive probe for the detection of Cys4-tagged proteins.
abstract: Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys-Cys-X-X-Cys-Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized (129)Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the (129)Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.
authors.affiliation: iBiTec-S/SCBM, LabEx LERMIT, CEA Saclay, 91191 Gif-sur-Yvette, France. bernard.rousseau@cea.fr.
authors.forename: N
authors.initials: N
authors.lastname: Kotera
authors.forename: E
authors.initials: E
authors.lastname: Dubost
authors.forename: G
authors.initials: G
authors.lastname: Milanole
authors.forename: E
authors.initials: E
authors.lastname: Doris
authors.forename: E
authors.initials: E
authors.lastname: Gravel
authors.forename: N
authors.initials: N
authors.lastname: Arhel
authors.forename: T
authors.initials: T
authors.lastname: Brotin
authors.forename: J-P
authors.initials: JP
authors.lastname: Dutasta
authors.forename: J
authors.initials: J
authors.lastname: Cochrane
authors.forename: E
authors.initials: E
authors.lastname: Mari
authors.forename: C
authors.initials: C
authors.lastname: Boutin
authors.forename: E
authors.initials: E
authors.lastname: Léonce
authors.forename: P
authors.initials: P
authors.lastname: Berthault
authors.forename: B
authors.initials: B
authors.lastname: Rousseau
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D003545:Cysteine / Q000032:analysis*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D011083:Polycyclic Compounds / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014979:Xenon Isotopes / Q000737:chemistry
chemical_list: C518706:CrAsH compound; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010455:Peptides; D011083:Polycyclic Compounds; D011994:Recombinant Proteins; D014979:Xenon Isotopes; C523242:cryptophane; C024098:6-carboxyfluorescein; D003545:Cysteine