title: Comparison of cell death and accumulation of reactive oxygen species in wheat lines with or without Yr36 responding to Puccinia striiformis f. sp. tritici under low and high temperatures at seedling and adult-plant stages.
abstract: Yr36 is an important gene conferring resistance to stripe rust of wheat caused by Puccinia striiformis f. sp. tritici (Pst). To determine if the Yr36 resistance is correlated to reactive oxygen species (ROS) burst and cell death, wheat near-isogenic lines with (UC1041 + Yr36) and without (UC1041) the gene were histologically characterized for response to Pst infection. Yr36 conferred stripe rust resistance at both seedling and adult-plant stages when the gene line was tested with Pst race CYR29 at a high-temperature (HT) cycle (12 °C at night and 33 °C during the day). At the HT cycle, the growth of secondary hyphae was obviously suppressed in both seedlings and adult plants of UC1041 + Yr36 compared with those of UC1041. The percentages of infection sites with necrotic host cells in UC1041 + Yr36 were significantly higher than UC1041 60 hours after inoculation (hai) at both seedling and adult-plant stages. Mesophyll cell death in the inoculated UC1041 + Yr36 leaves at the HT cycle was stronger than at a low-temperature (LT) cycle (12 °C at night and 18 °C during the day). At the HT cycle, the level of ROS burst started increasing in the inoculated leaves of UC1041 + Yr36 when Pst hyphae started differentiating and extending, and simultaneously, the number of penetration sites with hypersensitive cell death was also increasing. The results indicate that Yr36 product affects the ROS accumulation and cell death of the host in interaction of wheat with Pst.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. huanglili1@hotmail.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Huang
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity*; D016923:Cell Death; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D054884:Host-Pathogen Interactions; D006861:Hydrogen Peroxide / Q000378:metabolism; D058503:Mesophyll Cells / Q000382:microbiology / Q000473:pathology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D013696:Temperature; D014908:Triticum / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Hypersensitive response; Plant disease resistance; Puccinia striiformis; Reactive oxygen species; Triticum aestivum