title: Detection of urine metabolites in polycystic ovary syndrome by UPLC triple-TOF-MS.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is an endocrine disorder whose pathogenesis remains unclear. There are also no effective biomarkers for this disease. We evaluated the metabolic changes in PCOS patients and to investigate potential metabolic biomarkers for PCOS.
  
  
  METHODS
  Twenty-two women with PCOS and 15 healthy controls were studied. Urine samples were assessed through ultra-performance liquid chromatography-mass spectrometry followed by principal component analysis and partial least squares discriminant analysis.
  
  
  RESULTS
  Using the presented methods, 59 urine metabolites were found at different concentrations in PCOS patients. Moreover, two novel potential biomarkers, testosterone-glucuronide and 11α-hydroxyprogesterone, and four candidate biomarkers, benzofenap, methionyl-phenylalanine, MG(18:4(6Z,9Z,12Z,15Z)/0:0/0:0) and 2-(14,15-epoxyeicosatrienoyl) glycerol, were found to show significant differences through variance analysis (P<0.01) and were identified as target metabolites. The two potential biomarkers identified in this study highly correlate to the metabolites catalyzed by the ovarian cytochrome P450c17α.
  
  
  CONCLUSIONS
  Two novel potential urinary biomarkers, testosterone-glucuronide and 11α-hydroxyprogesterone, and four candidate urinary biomarkers, benzofenap, methionyl-phenylalanine, MG(18:4(6Z,9Z,12Z,15Z)/0:0/0:0), and 2-(14,15-epoxyeicosatrienoyl) glycerol, were identified in PCOS patients by a metabolomic approach. Further study of the biomarkers using larger populations is needed to validate these biomarkers and thereby understand the pathogenesis of PCOS, potentially allowing for its diagnosis.
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Wencui
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Shenglin
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Tan
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Meiqin
authors.initials: M
authors.lastname: Wen
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Life Sciences, School of Clinical Medicine, Chongqing Medical University, Chongqing 400016, P. R. China. Electronic address: yuchaom@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D011085:Polycystic Ovary Syndrome / Q000378:metabolism / Q000652:urine*; D025341:Principal Component Analysis; D053719:Tandem Mass Spectrometry; D016482:Urinalysis; D055815:Young Adult
keywords: Biomarkers; Metabolomics; Polycystic ovary syndrome; Urine