title: Regulation of human mesenchymal stem cell differentiation by TREM-2.
abstract: Activation of the triggering receptor expressed on myeloid cells 2 (TREM-2) regulates myeloid cell function in vitro. However, the failure to detect TREM-2 protein expression in vivo has hampered studies on immunological and other physiological TREM-2 functions. This study demonstrates that TREM-2 is expressed by human mesenchymal stem cells (h-MSCs) and responds to the toll-like receptor (TLR) ligand lipopolysaccharide (LPS). Knockdown of TREM-2 in h-MSCs using a small interfering RNA (siRNA) reduced the expression levels of TLR2, TLR4, and TLR6, inhibited osteogenic, chondrogenic, and adipogenic differentiation under specific induction conditions, and enhanced LPS-evoked inflammatory cytokine production. Thus, activation of TREM-2 may restrain h-MSC immune activation and promote differentiation for tissue repair.
authors.affiliation: Department of Orthopedics, The Sun Yat Sen Memory Hospital, Sun Yat Sen University, Guangzhou 510120, China; Department of Orthopedics, Zeng Cheng People's Hospital, Guangzhou 511300, China.
authors.forename: Wei-Qiong
authors.initials: WQ
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The Sun Yat Sen Memory Hospital, Sun Yat Sen University, Guangzhou 510120, China; Department of Orthopedics, Zeng Cheng People's Hospital, Guangzhou 511300, China.
authors.forename: Sheng-Hui
authors.initials: SH
authors.lastname: Huang
authors.affiliation: Department of Immunology, Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.affiliation: Physiological Department of Guangzhou Medical University, Guangzhou 510080, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Li
authors.affiliation: Department of Orthopedics, The Sun Yat Sen Memory Hospital, Sun Yat Sen University, Guangzhou 510120, China; Department of Orthopedics, Zeng Cheng People's Hospital, Guangzhou 511300, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Ye
authors.affiliation: Department of Orthopedics, Zeng Cheng People's Hospital, Guangzhou 511300, China.
authors.forename: Jinhuang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Zeng Cheng People's Hospital, Guangzhou 511300, China.
authors.forename: Pei-Zhong
authors.initials: PZ
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, The Sun Yat Sen Memory Hospital, Sun Yat Sen University, Guangzhou 510120, China.
authors.forename: Hui-Yong
authors.initials: HY
authors.lastname: Shen
authors.affiliation: Department of Orthopedics, The Sun Yat Sen Memory Hospital, Sun Yat Sen University, Guangzhou 510120, China; Department of Orthopedics, Zeng Cheng People's Hospital, Guangzhou 511300, China. Electronic address: guke16@163.com.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Huang
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010012:Osteogenesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011971:Receptors, Immunologic / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D014945:Wound Healing
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; D011971:Receptors, Immunologic; C498715:TREM2 protein, human; D051193:Toll-Like Receptors
keywords: Human mesenchymal stem cells (h-MSCs); TREM-2 (triggering receptor expressed on myeloid cells 2); Toll-like receptors (TLRs)