title: [NRP-1/shRNA silencing suppresses nasopharyngeal carcinoma cell growth in vitro and in vivo].
abstract: OBJECTIVE
  To investigate the effect of RNA interference (RNAi) targeting NRP-1 gene on the growth and proliferation of nasopharyngeal carcinoma (NPC) CNE-2Z cells.
  
  
  METHODS
  Short hairpin RNA (shRNA) plasmids targeting NRP-1 were designed and synthesized. These plasmids were respectively transfected into human NPC CNE-2Z cells and xenograft tumors in nude mice. The expressions of Fluorescein-labeled plasmids in NPC CNE-2Z cells and xenograft tumors were observed by fluorescence microscopy. Cell proliferation was detected by MTT assay. The inhibitory effects on target genes were evaluated with RT-PCR and Western blotting, respectively. The inhibitory effect of plasmids with the most effective sequence on xenograft tumors in nude mice was observed.
  
  
  RESULTS
  CNE-2Z cell proliferation was significantly inhibited by NRP-1/shRNA silencing. RT-PCR showed NRP-1 mRNA expression was significantly decreased. Western blotting demonstrated the NRP-1/shRNA silencing can effectively inhibit the expression of target proteins in CNE-2Z cells. After six weeks, there were significant differences in the mean tumor volumes in nude mice between plasmid group and negative control group [(0.599±0.002) vs (1.141±0.013) cm(3), P<0.05] or blank control group [(0.599±0.002) vs (1.165±0.308) cm(3), P<0.05], and the inhibitory rate of tumor growth was 48.6%.
  
  
  CONCLUSIONS
  RNA interference targeting NRP-1 can remarkably inhibit the growth of CNE-2Z cells in vitro and in vivo.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Email: wangliangdoctor@hotmail.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Lou
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xiufen
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Sang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002277:Carcinoma; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D039942:Neuropilin-1 / Q000378:metabolism*; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D039942:Neuropilin-1