title: Genome-wide transcriptome profiling reveals novel insights into Luffa cylindrica browning.
abstract: Luffa cylindrica (sponge gourd) is one of the most popular vegetables in China. Production and consumption of L. cylindrica are limited due to postharvest browning; however, little is known about the genetic regulation of the browning process. In the present study, transcriptome profiles of L. cylindrica cultivars, YLB05 (browning resistant) and XTR05 (browning sensitive), were analyzed using next-generation sequencing to clarify the genes and mechanisms associated with browning. A total of 9.1 Gb of valid data including 116,703 unigenes (>200 bp) were obtained and 39,473 sequences were annotated by alignment against five public databases. Of these, there were 27,407 genes assigned to 747 Gene Ontology functional categories; and 12,350 genes were annotated with 25 Eukaryotic Orthologous Groups (KOG) categories with 343 KOG functional terms. Additionally, by searching against the Kyoto Encyclopedia of Genes and Genomes database, 8689 unigenes were mapped to 189 pathways. Furthermore, there were 24,556 sequences found to be differentially regulated, including 4344 annotated unigenes. Several genes potentially associated with phenolic oxidation, carbohydrate and hormone metabolism were found differentially regulated between the cultivars of different browning sensitivities. Our results suggest that elements involved in enzymatic processes and other pathways might be responsible for L. cylindrica browning. The present study provides a comprehensive transcriptome sequence resource, which will facilitate further studies on gene discovery and exploiting the fruit browning mechanism of L. cylindrica.
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China; Key Laboratory of Horticultural Plant Biology, Ministry of Education, National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China. Electronic address: tantaiming_001@126.com.
authors.forename: Taiming
authors.initials: T
authors.lastname: Tan
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Huang
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tan
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei 430065, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Ministry of Education, National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xiech@mail.hzau.edu.cn.
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
mesh_terms: D018745:Genome, Plant; D029776:Luffa / Q000235:genetics* / Q000502:physiology; D059467:Transcriptome
keywords: Browning; Differentially expressed genes; Luffa cylindrica; Transcriptome profile