title: Proteome catalog of Zymoseptoria tritici captured during pathogenesis in wheat.
abstract: Zymoseptoria tritici is an economically important pathogen of wheat. However, the molecular basis of pathogenicity on wheat is still poorly understood. Here, we present a global survey of the proteins secreted by this fungus in the apoplast of resistant (cv. Shafir) and susceptible (cv. Obelisk) wheat cultivars after inoculation with reference Z. tritici strain IPO323. The fungal proteins present in apoplastic fluids were analyzed by gel electrophoresis and by data-independent acquisition liquid chromatography/mass spectrometry (LC/MS(E)) combined with data-dependent acquisition LC-MS/MS. Subsequent mapping mass spectrometry-derived peptide sequence data against the genome sequence of strain IPO323 identified 665 peptides in the MS(E) and 93 in the LC-MS/MS mode that matched to 85 proteins. The identified fungal proteins, including cell-wall degrading enzymes and proteases, might function in pathogenicity, but the functions of many remain unknown. Most fungal proteins accumulated in cv. Obelisk at the onset of necrotrophy. This inventory provides an excellent basis for future detailed studies on the role of these genes and their encoded proteins during pathogenesis in wheat.
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands; Laboratory of Plant Molecular Physiology, Biotechnology Center of Borj-Cedria, BP 901 Hammam-Lif-2050, Tunisia.
authors.forename: Sarrah
authors.initials: S
authors.lastname: Ben M'Barek
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands; Center for BioSystems and Genomics, 6700AA Wageningen, The Netherlands; Netherlands Proteomics Center, Utrecht, The Netherlands.
authors.forename: Jan H G
authors.initials: JH
authors.lastname: Cordewener
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands.
authors.forename: Theo A J
authors.initials: TA
authors.lastname: van der Lee
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands; Center for BioSystems and Genomics, 6700AA Wageningen, The Netherlands; Netherlands Proteomics Center, Utrecht, The Netherlands.
authors.forename: Antoine H P
authors.initials: AH
authors.lastname: America
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands; Department of Plant Protection, College of Agriculture, University of Tehran, Plant Pathology Building, Karaj, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Mirzadi Gohari
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands; Cereal Research Department, Seed and Plant Improvement Institute, Karaj, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mehrabi
authors.affiliation: Laboratory of genetics, National Agronomic Institute of Tunisia, 43 Avenue Charles Nicolle, 1082 Tunis, Tunisia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Hamza
authors.affiliation: Laboratory of Phytopathology, Wageningen University, 6708PB Wageningen, The Netherlands.
authors.forename: Pierre J G M
authors.initials: PJ
authors.lastname: de Wit
authors.affiliation: Wageningen University & Research Center, Plant Research International, 6708PB Wageningen, The Netherlands. Electronic address: gert.kema@wur.nl.
authors.forename: Gerrit H J
authors.initials: GH
authors.lastname: Kema
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D004586:Electrophoresis; D005656:Fungal Proteins / Q000032:analysis*; D013058:Mass Spectrometry; D010935:Plant Diseases / Q000382:microbiology*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins; D020543:Proteome
keywords: Apoplastic fluid; Fungal proteins; Liquid chromatography/mass spectrometry; Pathogenesis; Proteomics; Zymoseptoria tritici