title: New easy handling and sampling device for bioavailability screening of topical formulations.
abstract: Biopharmaceutical assessment of topical drug formulations is widely carried out by using vertical diffusion cells (Franz type cell). Although Franz diffusion cell model is well designed for percutaneous absorption studies, the extent of drug penetration within the skin requires more adapted device. Recently, we have developed a new patented versatile, easy-to-use, and disposable diffusion cell called VitroPharma. In this study we have assessed the cutaneous bioavailability of caffeine as hydrophilic compound model using Franz diffusion cell and VitroPharma. The percutaneous absorption of caffeine assessed with Franz diffusion cell and VitroPharma was characterized by using (i) finite dose model and (ii) classical pharmacokinetic analysis. Furthermore, the follow-up of caffeine penetration within the skin was determined by sequential measurements of tissular drug concentration throughout the time of skin exposure with VitroPharma. However, classical experimental design using Franz diffusion cell involved unique determination of tissular concentration at the final point of skin exposure protocol. Finally, device equivalence between Franz diffusion cell and VitroPharma was claimed from percutaneous absorption data analysis. Concomitant assessment of dual penetration and permeation kinetics by using VitroPharma reinforced the understanding of skin drug delivery.
authors.affiliation: EA 4169 : Aspect fondamentaux, cliniques et thérapeutiques de la fonction barrière cutanée, Laboratoire de Pharmacie Galénique Industrielle, Plateforme FRIPHARM, Faculté de Pharmacie, SFR Lyon-Est Santé, Université Claude Bernard Lyon 1. 8, avenue Rockefeller; 69373 Lyon cedex 08, France www.fripharm.com, Unité de préparation et contrôle des médicaments, Service Pharmaceutique, Plateforme FRIPHARM, Groupement Hospitalier Edouard Herriot, Hospices Civils de Lyon. 5, Place d'Arsonval ; 69437 Lyon cedex 03, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Salmon
authors.affiliation: EA 4169 : Aspect fondamentaux, cliniques et thérapeutiques de la fonction barrière cutanée, Laboratoire de Pharmacie Galénique Industrielle, Plateforme FRIPHARM, Faculté de Pharmacie, SFR Lyon-Est Santé, Université Claude Bernard Lyon 1. 8, avenue Rockefeller; 69373 Lyon cedex 08, France www.fripharm.com.
authors.forename: Elodie
authors.initials: E
authors.lastname: Gilbert
authors.affiliation: Unité de préparation et contrôle des médicaments, Service Pharmaceutique, Plateforme FRIPHARM, Groupement Hospitalier Edouard Herriot, Hospices Civils de Lyon. 5, Place d'Arsonval ; 69437 Lyon cedex 03, France.
authors.forename: Bruna
authors.initials: B
authors.lastname: Gioia
authors.affiliation: EA 4169 : Aspect fondamentaux, cliniques et thérapeutiques de la fonction barrière cutanée, Laboratoire de Pharmacie Galénique Industrielle, Plateforme FRIPHARM, Faculté de Pharmacie, SFR Lyon-Est Santé, Université Claude Bernard Lyon 1. 8, avenue Rockefeller; 69373 Lyon cedex 08, France www.fripharm.com.
authors.forename: Marek
authors.initials: M
authors.lastname: Haftek
authors.affiliation: Unité de préparation et contrôle des médicaments, Service Pharmaceutique, Plateforme FRIPHARM, Groupement Hospitalier Edouard Herriot, Hospices Civils de Lyon. 5, Place d'Arsonval ; 69437 Lyon cedex 03, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Pivot
authors.affiliation: UMR 5305 : Laboratoire de Biologie Tissulaire et d'Ingénierie Thérapeutique, CNRS/Université Claude Bernard Lyon 1, Institut de Biologie et Chimie des Protéines. 7, Passage du Vercors ; 69367 Lyon Cedex 07, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Verrier
authors.affiliation: EA 4169 : Aspect fondamentaux, cliniques et thérapeutiques de la fonction barrière cutanée, Laboratoire de Pharmacie Galénique Industrielle, Plateforme FRIPHARM, Faculté de Pharmacie, SFR Lyon-Est Santé, Université Claude Bernard Lyon 1. 8, avenue Rockefeller; 69373 Lyon cedex 08, France www.fripharm.com, Unité de préparation et contrôle des médicaments, Service Pharmaceutique, Plateforme FRIPHARM, Groupement Hospitalier Edouard Herriot, Hospices Civils de Lyon. 5, Place d'Arsonval ; 69437 Lyon cedex 03, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pirot
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001682:Biological Availability; D002110:Caffeine / Q000032:analysis / Q000493:pharmacokinetics; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation*; D015194:Diffusion Chambers, Culture; D012869:Skin Absorption; D013552:Swine
chemical_list: D002110:Caffeine
keywords: Franz diffusion cell; VitroPharma; caffeine; permeability assay; porcine skin