title: Antihepatic Fibrosis Effect of Active Components Isolated from Green Asparagus (Asparagus officinalis L.) Involves the Inactivation of Hepatic Stellate Cells.
abstract: Green asparagus (Asparagus officinalis L.) is a vegetable with numerous nutritional properties. In the current study, a total of 23 compounds were isolated from green asparagus, and 9 of these compounds were obtained from this genus for the first time. Preliminary data showed that the ethyl acetate (EtOAc)-extracted fraction of green asparagus exerted a stronger inhibitory effect on the growth of t-HSC/Cl-6 cells, giving an IC50 value of 45.52 μg/mL. The biological activities of the different compounds isolated from the EtOAc-extracted fraction with respect to antihepatic fibrosis were investigated further. Four compounds, C3, C4, C10, and C12, exhibited profound inhibitory effect on the activation of t-HSC/Cl-6 cells induced by TNF-α. The activation t-HSC/Cl-6 cells, which led to the production of fibrotic matrix (TGF-β1, activin C) and accumulation of TNF-α, was dramatically decreased by these compounds. The mechanisms by which these compounds inhibited the activation of hepatic stellate cells appeared to be associated with the inactivation of TGF-β1/Smad signaling and c-Jun N-terminal kinases, as well as the ERK phosphorylation cascade.
authors.affiliation: †College of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Chunge
authors.initials: C
authors.lastname: Zhong
authors.forename: Chunyu
authors.initials: C
authors.lastname: Jiang
authors.affiliation: †College of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Xichun
authors.initials: X
authors.lastname: Xia
authors.affiliation: †College of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Mu
authors.forename: Lige
authors.initials: L
authors.lastname: Wei
authors.forename: Yuntian
authors.initials: Y
authors.lastname: Lou
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: †College of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Bi
mesh_terms: D000818:Animals; D027761:Asparagus Plant / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D051785:Smad Proteins; D016212:Transforming Growth Factor beta
keywords: Asparagus officinalis L; ERK; TGF-β1/Smad signaling; active components; antihepatic fibrosis; c-Jun N-terminal kinases