title: Effects of Immunoregulatory Cytokines (IL-2, IL-7, and IL-15) on Expression of Gfi1 and U2afll4 Genes in T Cells at Different Stages of Differentiation.
abstract: Opposite effects of common γ-chain cytokines (IL-2, IL-7, and IL-15) on the expression of Gfi1 and U2af1l4 genes regulating alternative splicing of Ptprc gene in T cells at different stages of differentiation were demonstrated in vitro. Generally, produced a dose-dependent activating effect on T cells, while to the effects of rIL-7 and rIL-15 on T cells at different stages of differentiation were opposite to that of rIL-2: maximum concentrations of recombinant cytokines IL-7 and IL-15 produced the most pronounced inhibitory effect on U2af1l4 and to a lesser extent on Gfi1 gene expression, thus limiting activation of resting cells. This is consistent with their biological effects on T cells. In general, common γ-chain cytokines IL-2, IL-7, and IL-15 prevent differentiation of naïve T cells in vitro and limit activation of primed T cells in the absence of antigenic stimulus, which can contribute to the formation of cytokine imbalance.
authors.affiliation: Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Sokhonevich
authors.forename: K A
authors.initials: KA
authors.lastname: Yurova
authors.forename: A A
authors.initials: AA
authors.lastname: Gutsol
authors.forename: O G
authors.initials: OG
authors.lastname: Khaziakhmatova
authors.forename: I O
authors.initials: IO
authors.lastname: Mazunin
authors.forename: L S
authors.initials: LS
authors.lastname: Litvinova
mesh_terms: D000328:Adult; D017398:Alternative Splicing / Q000187:drug effects*; D002454:Cell Differentiation / Q000276:immunology*; D016207:Cytokines / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D018189:Immunomagnetic Separation; D019409:Interleukin-15 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000235:genetics; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D000072668:Splicing Factor U2AF; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004268:DNA-Binding Proteins; C498275:GFI1 protein, human; D019409:Interleukin-15; D007376:Interleukin-2; D015851:Interleukin-7; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; D014157:Transcription Factors; C486290:U2AF1L4 protein, human; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human