title: Naive and radiolabeled antibodies to E6 and E7 HPV-16 oncoproteins show pronounced antitumor activity in experimental cervical cancer.
abstract: BACKGROUND
  In spite of profound reduction in incidence, cervical cancer claims >275,000 lives annually. Previously we demonstrated efficacy and safety of radioimmunotherapy directed at HPV16 E6 oncoprotein in experimental cervical cancer.
  
  
  METHODS
  We undertook a direct comparison of targeting E7 and E6 oncoproteins with specific (188)Rhenium-labeled monoclonal antibodies in CasKi subcutaneous xenografts of cervical cancer cells in mice.
  
  
  RESULTS
  The most significant tumor inhibition was seen in radioimmunotherapy-treated mice, followed by the unlabeled monoclonal antibodies to E6 and E7. No hematological toxicity was observed. Immunohistochemistry suggests that the effect of unlabeled antibodies is C3 complement mediated.
  
  
  CONCLUSIONS
  We have demonstrated for the first time that radioimmunotherapy directed toward E7 oncoprotein inhibits experimental tumors growth, decreases E7 expression and may offer a novel approach to cervical cancer therapy.
authors.affiliation: Department of Obstetrics & Gynecology, Albert Einstein College of Medicine, NY, USA.
authors.forename: R
authors.initials: R
authors.lastname: Phaëton
authors.affiliation: Department of Obstetrics & Gynecology, Albert Einstein College of Medicine, NY, USA.
authors.forename: J
authors.initials: J
authors.lastname: Gutierrez
authors.affiliation: Department of Radiology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, NY, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Karabakhtsian
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, NY, USA.
authors.forename: J
authors.initials: J
authors.lastname: Albanese
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, NY, USA.
authors.forename: J
authors.initials: J
authors.lastname: Sunkara
authors.affiliation: Dade Moeller Health Group, WA, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Fisher
authors.affiliation: Department of Obstetrics & Gynecology, Albert Einstein College of Medicine, NY, USA.
authors.forename: G L
authors.initials: GL
authors.lastname: Goldberg
authors.affiliation: Department of Radiology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA.
authors.forename: E
authors.initials: E
authors.lastname: Dadachova
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000276:immunology / Q000532:radiotherapy* / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D050725:Papillomavirus E7 Proteins / Q000276:immunology*; D030361:Papillomavirus Infections / Q000276:immunology / Q000628:therapy*; D016499:Radioimmunotherapy; D012097:Repressor Proteins / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000532:radiotherapy* / Q000821:virology
chemical_list: D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16
keywords: 188Rhenium; E6 and E7 oncoproteins; HPV16 positive cervical cancer; apoptosis; complement cytotoxicity; p53 expression; radioimmunotherapy; retinoblastoma