title: Optimization for ultrasound extraction of polysaccharides from mulberry fruits with antioxidant and hyperglycemic activity in vitro.
abstract: Single-factor experiment and Box-Behnken design (BBD) were applied to optimize the ultrasound-assisted extraction of mulberry fruits polysaccharides (MFP). Under optimum conditions: ratio of water to raw material 40.25, extraction temperature 69°C, ultrasonic power 190W and extraction time 75 min, the MFP yield was 3.13% (±0.07%), in accordance to the predicted value of 3.04%. The mulberry fruits polysaccharides fractions was obtained by deproteinization (MFP-1), followed by decolorization and deionization (MFP-2). Carbohydrate content in MFP, MFP-1 and MFP-2 was 58.61% (±1.47%), 69.98% (±0.91%), 81.18% (±1.29%), as well as proteins was estimated 16.50% (±0.86%), 1.57% (±0.63%), 1.02% (±0.18%), respectively. The FT-IR indicated that MFP, MFP-1 and MFP-2 were acidic polysaccharides. The MFP-1 exhibited the strongest antioxidant activity, while MFP-2 showed the strongest hyperglycemic activity in vitro. This may be caused by their different compositions and physical properties in the different mulberry fruit polysaccharides fractions.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: You
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
authors.forename: Arshad Mehmood
authors.initials: AM
authors.lastname: Abbasi
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China. Electronic address: lfxfu@scut.edu.cn.
authors.forename: Xiong
authors.initials: X
authors.lastname: Fu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Rui Hai
authors.initials: RH
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005591:Chemical Fractionation / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D008956:Models, Chemical; D009005:Monosaccharides / Q000032:analysis; D031625:Morus / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D014465:Ultrasonics / Q000379:methods; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D009005:Monosaccharides; D011134:Polysaccharides; D017665:Hydroxyl Radical; D000520:alpha-Glucosidases
keywords: Antioxidant activity; Hypoglycemic activity; Morus alba; Mulberry fruit; Polysaccharides; Ultrasound-assisted extraction