title: Rhamnolipid production, characterization and fermentation scale-up by Pseudomonas aeruginosa with plant oils.
abstract: OBJECTIVE
  To be competitive with common chemical surfactants, the cost of rhamnolipid production must be minimized by selecting suitable substrates and optimizing the fermentation process.
  
  
  RESULTS
  With different plant oils as substrates, Pseudomonas aeruginosa TIB-R02 can produce rhamnolipids with different structural characteristics that were confirmed by HPLC/MS analysis. Different rhamnolipids had different performances in interfacial tension. The production of rhamnolipid was greatly enhanced by fermentation optimization with palm oil as substrate. A fermentation-defoaming tandem system was developed to resolve the problems of foaming and medium overflow during scale-up. Finally, the titer of rhamnolipid reached 60 g/l and the yield reached 80% in a 300 l fermentation-defoaming tandem system.
  
  
  CONCLUSIONS
  The work reveals the potential for producing high-performance rhamnolipids from renewable resources on a large scale.
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin Airport Economic Area, Tianjin, 300308, China. gong_zj@tib.cas.cn.|College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China. gong_zj@tib.cas.cn.
authors.forename: Zhijin
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin Airport Economic Area, Tianjin, 300308, China. peng_yf@tib.cas.cn.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin Airport Economic Area, Tianjin, 300308, China. wang_qh@tib.cas.cn.
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006017:Glycolipids / Q000378:metabolism*; D013058:Mass Spectrometry; D010938:Plant Oils / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D010938:Plant Oils; C418382:rhamnolipid
keywords: Fermentation-defoaming; Interfacial tension; Plant oils; Pseudomonas aeruginosa; Rhamnolipids; Scale-up