title: Elevated Serum MicroRNA Let-7c in Moyamoya Disease.
abstract: BACKGROUND
  Few studies have examined the relationship between mircroRNAs and moyamoya disease (MMD). We performed a study of the significance of let-7c expression in the serum of MMD patients.
  
  
  METHODS
  The experimental group includes 49 MMD patients, and the control group consists of 30 normal people, 20 cerebral hemorrhage patients, 20 massive cerebral infarction patients, 20 nonmassive cerebral infarction patients, and 20 neurological autoimmune disease patients. Let-7 family levels were determined by polymerase chain reaction. A dual luciferase assay was used to test whether let-7c recognized the 3'UTR of RNF213.
  
  
  RESULTS
  The expression level of let-7c in MMD patients is higher than that observed in the control groups (P < .001). The luciferase assay results indicated that hsa-let-7c could diminish luciferase activity from a reporter vector containing the 3'-UTR of RNF213 (P < .05). The suppression of luciferase activity is not found in mutRNF213 (P > .05).
  
  
  CONCLUSIONS
  Increased expression of let-7c in MMD patients may contribute to MMD pathogenesis by targeting RNF213. Thus, let-7c may be a potential biomarker for the diagnosis of MMD.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Department of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Shaoyun
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Department of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Department of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Department of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Peidong
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Guan
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Teng
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Electronic address: jiayanjie1971@aliyun.com.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000328:Adult; D001327:Autoimmune Diseases / Q000097:blood; D002543:Cerebral Hemorrhage / Q000097:blood; D002544:Cerebral Infarction / Q000097:blood; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009072:Moyamoya Disease / Q000097:blood*; D012333:RNA, Messenger / Q000378:metabolism; D018709:Statistics, Nonparametric; D014162:Transfection
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; C487931:mirnlet7 microRNA, human
keywords: Moyamoya disease; PCR; RNF213; let-7c