title: Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis).
abstract: This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a 'small round cell' undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings.
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy. Electronic address: rubina.sirri2@unibo.it.
authors.forename: R
authors.initials: R
authors.lastname: Sirri
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bianco
authors.affiliation: Department of Food Science, Section Veterinary Pathology, University of Udine, Via Sondrio 2/a, 33100 Udine, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Beraldo
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mandrioli
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Pulvirenti
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Brachelente
authors.affiliation: Department of Food Science, Section Veterinary Pathology, University of Udine, Via Sondrio 2/a, 33100 Udine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Galeotti
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Sarli
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D005393:Fish Diseases / Q000473:pathology*; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D012208:Rhabdomyosarcoma / Q000473:pathology / Q000662:veterinary*; D012983:Soft Tissue Neoplasms / Q000473:pathology / Q000662:veterinary*; D014337:Trout
chemical_list: D014408:Biomarkers, Tumor
keywords: immunohistochemistry; protein blast; rhabdomyosarcoma; salmonid