title: Regulation of BAX/BCL2 gene expression in breast cancer cells by docetaxel-loaded human serum albumin nanoparticles.
abstract: Today, using nanoparticle-based drug delivery systems has expanded to avoid anticancer side effects. Taxanes are important chemotherapeutic agents in the treatment of metastatic breast cancer. In this study, docetaxel (DTX)-loaded human serum albumin (HSA) nanoparticles (NPs) were prepared and characterized. Drug toxicity of the nanoparticles was measured by MTT assay with different drug concentrations (0.01, 0.1, 0.5, 1 and 5 μM) at different incubation times (24, 48 and 72 h). Expression of BAX/BCL2 mRNA levels was determined by real-time PCR. The size of NPs prepared and used in our study was about 147 nm with surface charge of -29.6 mV. Results obtained from MTT assay showed that 0.5 μM of free drug had 50 % toxicity on MCF-7 cells after 48-h incubation. Real-time PCR results showed an increase in expression of BAX and no change for BCL2. In conclusion, a significant overexpression of BAX gene and changes in BAX/BCL2 ratio were observed for DTX-loaded HSA nanoparticles compared with free DTX and may provide a potential therapy to inhibit anticancer drug resistance.
authors.affiliation: Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Kordezangeneh
authors.forename: Shiva
authors.initials: S
authors.lastname: Irani
authors.forename: Reza
authors.initials: R
authors.lastname: Mirfakhraie
authors.forename: Mehdi
authors.initials: M
authors.lastname: Esfandyari-Manesh
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atyabi
authors.forename: Rassoul
authors.initials: R
authors.lastname: Dinarvand
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics; D045744:Cell Line, Tumor; D000077143:Docetaxel; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000008:administration & dosage*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012709:Serum Albumin / Q000008:administration & dosage*; D043823:Taxoids / Q000008:administration & dosage*; D051028:bcl-2-Associated X Protein / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D012709:Serum Albumin; D043823:Taxoids; D051028:bcl-2-Associated X Protein; D000077143:Docetaxel