title: Leaf cDNA-AFLP analysis reveals novel mechanisms for boron-induced alleviation of aluminum-toxicity in Citrus grandis seedlings.
abstract: Little information is available on the molecular mechanisms of boron (B)-induced alleviation of aluminum (Al)-toxicity. 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing different concentrations of B (2.5 or 20μM H3BO3) and Al (0 or 1.2mM AlCl3·6H2O). B alleviated Al-induced inhibition in plant growth accompanied by lower leaf Al. We used cDNA-AFLP to isolate 127 differentially expressed genes from leaves subjected to B and Al interactions. These genes were related to signal transduction, transport, cell wall modification, carbohydrate and energy metabolism, nucleic acid metabolism, amino acid and protein metabolism, lipid metabolism and stress responses. The ameliorative mechanisms of B on Al-toxicity might be related to: (a) triggering multiple signal transduction pathways; (b) improving the expression levels of genes related to transport; (c) activating genes involved in energy production; and (d) increasing amino acid accumulation and protein degradation. Also, genes involved in nucleic acid metabolism, cell wall modification and stress responses might play a role in B-induced alleviation of Al-toxicity. To conclude, our findings reveal some novel mechanisms on B-induced alleviation of Al-toxicity at the transcriptional level in C. grandis leaves.
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Wang
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Lin-Tong
authors.initials: LT
authors.lastname: Yang
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Xin-Xing
authors.initials: XX
authors.lastname: Zhou
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: En-Jun
authors.initials: EJ
authors.lastname: Chen
authors.affiliation: College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: lisongchen2002@hotmail.com.
authors.forename: Li-Song
authors.initials: LS
authors.lastname: Chen
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D001895:Boron / Q000494:pharmacology*; D002957:Citrus / Q000737:chemistry / Q000187:drug effects*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D050356:Lipid Metabolism / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015203:Reproducibility of Results; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D018076:DNA, Complementary; D017382:Reactive Oxygen Species; D000535:Aluminum; D001895:Boron
keywords: Aluminum-toxicity; Boron; Boron and aluminum interactions; Citrus grandis; cDNA-AFLP