title: A calcium-binding protein, rice annexin OsANN1, enhances heat stress tolerance by modulating the production of H2O2.
abstract: OsANN1 is a member of the annexin protein family in rice. The function of this protein and the mechanisms of its involvement in stress responses and stress tolerance are largely unknown. Here it is reported that OsANN1 confers abiotic stress tolerance by modulating antioxidant accumulation under abiotic stress. OsANN1-knockdown [RNA interference (RNAi)] plants were more sensitive to heat and drought stresses, whereas OsANN1-overexpression (OE) lines showed improved growth with higher expression of OsANN1 under abiotic stress. Overexpression of OsANN1 promoted SOD (superoxide dismutase) and CAT (catalase) activities, which regulate H2O2 content and redox homeostasis, suggesting the existence of a feedback mechanism between OsANN1 and H2O2 production under abiotic stress. Higher expression of OsANN1 can provide overall cellular protection against abiotic stress-induced damage, and a significant accumulation of OsANN1-green fluorescent protein (GFP) signals was found in the cytosol after heat shock treatment. OsANN1 also has calcium-binding and ATPase activities in vitro, indicating that OsANN1 has multiple functions in rice growth. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays demonstrated that OsANN1 interacts with OsCDPK24. This cross-talk may provide additional layers of regulation in the abiotic stress response.
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Qiao
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Liu
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Haiqi
authors.initials: H
authors.lastname: Wang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Jingya
authors.initials: J
authors.lastname: Yin
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Mengli
authors.initials: M
authors.lastname: He
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Cui
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Shang
authors.affiliation: The Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China zhuzhengge@hebtu.edu.cn kay77@163.com.
authors.forename: Dekai
authors.initials: D
authors.lastname: Wang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang, Hebei, 050024, China zhuzhengge@hebtu.edu.cn kay77@163.com.
authors.forename: Zhengge
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D017302:Annexins / Q000235:genetics* / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D013312:Stress, Physiological
chemical_list: D017302:Annexins; D000975:Antioxidants; D010940:Plant Proteins
keywords: Annexin; Oryza sativa.; calcium binding activity; drought stress; heat stress; hydrogen peroxide