title: The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant.
abstract: H2S is a signaling molecule in plants and animals. Here we investigated the effects of H2S on programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers. The H2S donor NaHS significantly delayed PCD in aleurone layers isolated from imbibed embryoless barley grain. NaHS at 0.25 mM effectively reduced the accumulation of superoxide anion (·O2 (-)), hydrogen peroxide (H2O2), and malondialdehyde (MDA), promoted the activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and decreased those of lipoxygenase (LOX) in isolated aleurone layers. Quantitative-PCR showed that NaHS treatment of aleurone tissue led to enhanced transcript levels of the antioxidant genes HvSOD1, HvAPX, HvCAT1, and HvCAT2 and repressed transcript levels of HvLOX (lipoxygenase gene) and of two cysteine protease genes HvEPA and HvCP3-31. NaHS treatment in gibberellic acid- (GA-) treated aleurone layers also delayed the PCD process, reduced the content of ·O2 (-), and increased POD activity while decreasing LOX activity. Furthermore, α-amylase secretion in barley aleurone layers was enhanced by NaHS treatment regardless of the presence or absence of GA. These data imply that H2S acted as an antioxidant in delaying PCD and enhances α-amylase secretion regardless of the presence of GA in barley aleurone layers.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Ying-Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Kang-Di
authors.initials: KD
authors.lastname: Hu
authors.affiliation: Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lv
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Li
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Hu
authors.affiliation: Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Xi-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ruan
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Liu
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005875:Gibberellins / Q000494:pharmacology; D001467:Hordeum / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013440:Sulfides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000032:analysis; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005875:Gibberellins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013440:Sulfides; D013481:Superoxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; C007842:gibberellic acid; C108078:catalase I; D060387:Ascorbate Peroxidases; D002374:Catalase; D008084:Lipoxygenase; D013482:Superoxide Dismutase; D000516:alpha-Amylases; C025451:sodium bisulfide