title: Self-seeding microwell chip for the isolation and characterization of single cells.
abstract: A self-seeding microwell chip is introduced for the isolation and interrogation of single cells. A cell suspension is transferred to a microwell chip containing 6400 microwells, each microwell with a single 5 μm pore in the bottom. The fluid enters the microwell and drags a cell onto the pore. After a cell has landed onto the pore, it will stop the fluid flow through this microwell. The remaining fluid and cells will be diverted to the next available microwell. This results in a fast and efficient distribution of single cells in individual microwells. After identification by fluorescence microscopy, the cells of interest are isolated from the microwell by punching the bottom together with the cell. The overall single cell recovery of seeding followed by isolation of the single cell, is >70% with a specificity of 100% as confirmed by the genetic make-up of the isolated cells.
authors.affiliation: Medical Cell BioPhysics Group, MIRA Institute, University of Twente, Room CR4437, Hallenweg 23, 7522 NH, Enschede, The Netherlands. l.w.m.m.terstappen@utwente.nl.
authors.forename: Joost F
authors.initials: JF
authors.lastname: Swennenhuis
authors.forename: Arjan G J
authors.initials: AG
authors.lastname: Tibbe
authors.forename: Michiel
authors.initials: M
authors.lastname: Stevens
authors.forename: Madhumohan R
authors.initials: MR
authors.lastname: Katika
authors.forename: Joost
authors.initials: J
authors.lastname: van Dalum
authors.forename: Hien Duy
authors.initials: HD
authors.lastname: Tong
authors.forename: Cees J M
authors.initials: CJ
authors.lastname: van Rijn
authors.forename: Leon W M M
authors.initials: LW
authors.lastname: Terstappen
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation; D045744:Cell Line, Tumor; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008856:Microscopy, Fluorescence; D059010:Single-Cell Analysis