title: Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol A in human serum.
abstract: In the present work, a highly sensitive and selective biosensor based on aptamer-functionalized nanoporous gold film (NPGF) was successfully developed for direct electrochemical detection of bisphenol A (BPA). NPGF was prepared by dealloying Ag from Au/Ag alloy leaf in concentrated nitric acid. The obtained NPGF was attached onto glassy carbon electrode and then was functionalized with BPA-specific aptamer via the formation of Au-S bond. The fabrication of the sensor was characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. NPGF exhibited excellent electrocatalytic activity towards the redox reaction of BPA, which ensured high sensitivity of the sensor. The aptamer-captured BPA showed a pair of redox peaks around 0.35/0.28 V (vs. Ag/AgCl). The experimental parameters in terms of aptamer concentration, reaction time, pH, and temperature were optimized. The calibration plot showed a linear range from 0.1 nM to 100 nM BPA with a remarkable detection limit of 0.056±0.004 nM BPA. Particularly, the successful application of the developed sensor for the detection of BPA in human serum samples suggests its promising potential for clinical diagnosis.
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China. Electronic address: zhuye@sdu.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
authors.forename: Chuqing
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Yan
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Institute of New Energy Materials & Low-Carbon Technologies, Tianjin University of Technology, Tianjin 300384, China. Electronic address: qiangwang@tjut.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D052244:Endocrine Disruptors / Q000097:blood*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010636:Phenols / Q000097:blood*; D016062:Porosity; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D006046:Gold; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aptamer sensor; Bisphenol A; Direct electrochemical detection; Electrocatalysis; Nanoporous gold