title: High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep.
abstract: Heteroplasmy due to length polymorphism with tandem repeats in mtDNAs within individual was hardly studied in domestic animals. In the present study, we identified intra-individual length variation in the control region of mtDNAs in Nepalese sheep by molecular cloning and sequencing techniques. We observed one to four tandem repeats of a 75-bp nucleotide sequences in the mtDNA control region in 45% of the total Nepalese sheep sampled in contrast to the Chinese sheep, indicating that the heteroplasmy is specific to Nepalese sheep. The high rate of heteroplasmy in Nepalese sheep could be a resultant of the mtDNA mutation and independent segregation at intra-individual level or a strand slippage and mispairing during the replication.
authors.affiliation: a CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS) , Beijing , China .|b Animal Breeding Division, National Animal Science Institute, Nepal Agriculture Research Council (NARC) , Kathmandu , Nepal , and.
authors.forename: Neena Amatya
authors.initials: NA
authors.lastname: Gorkhali
authors.affiliation: a CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS) , Beijing , China .
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: b Animal Breeding Division, National Animal Science Institute, Nepal Agriculture Research Council (NARC) , Kathmandu , Nepal , and.
authors.forename: Bhola Shankar
authors.initials: BS
authors.lastname: Shrestha
authors.affiliation: a CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS) , Beijing , China .
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: He
authors.affiliation: a CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS) , Beijing , China .
authors.forename: Qian
authors.initials: Q
authors.lastname: Junzhao
authors.affiliation: a CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS) , Beijing , China .|c International Livestock Research Institute (ILRI) , Nairobi , Kenya.
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Han
authors.affiliation: a CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS) , Beijing , China .
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Ma
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D009154:Mutation; D012756:Sheep; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial
keywords: Heteroplasmy; Nepalese sheep; mtDNA control region; tandem repeats