title: HPLC-NMR and HPLC-MS Profiling and Bioassay-Guided Identification of Secondary Metabolites from the Australian Plant Haemodorum spicatum.
abstract: Phytochemical dereplication was undertaken on the bioactive crude CH2Cl2 extract of the bulbs of the Australian plant Haemodorum spicatum employing HPLC-NMR and HPLC-MS methodologies. Subsequent bioassay-guided isolation resulted in the identification of two new phenylphenalenones [haemoxiphidone (8) and haemodoronol (17)] and two new chromenes [haemodordione (13) and haemodordiol (16)], together with seven previously described compounds. Antimicrobial testing showed that the compounds displayed selective antibacterial activity. Most noteworthy were the activities displayed by several of the compounds against multi-drug-resistant Pseudomonas aeruginosa.
authors.affiliation: School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi), RMIT University, GPO Box 2476 V Melbourne, Victoria 3001, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Brkljača
authors.affiliation: School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi), RMIT University, GPO Box 2476 V Melbourne, Victoria 3001, Australia.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Urban
mesh_terms: D001315:Australia; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D019684:Magnoliopsida / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D043803:Phenalenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects
chemical_list: D001578:Benzopyrans; D043803:Phenalenes