title: Aptamer-based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection.
abstract: A novel aptamer-based CE with chemiluminescence (CL) assay was developed for highly sensitive detection of human immunoglobulin E (IgE). The IgE aptamer was conjugated with gold nanoparticles (AuNPs) to form AuNPs-aptamer that could specifically recognize the IgE to produce an AuNPs-aptamer-IgE complex. The mixture of the AuNPs-aptamer-IgE complex and the unbounded AuNPs-aptamer could be effectively separated by CE and sensitively detected with luminol-H2 O2 CL system. By taking the advantage of the excellent catalytic behavior of AuNPs on luminol-H2 O2 CL system, the ultrasensitive detection of IgE was achieved. The detection limit of IgE is 7.6 fM (S/N = 3) with a linear range from 0.025 to 250 pM. Successful detection of IgE in human serum samples was demonstrated and the recoveries of 94.9-103.2% were obtained. The excellent assay features of the developed approach are its specificity, sensitivity, adaptability, and very small sample consumption. Our design provides a methodology model for determination of rare proteins in biological samples.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, P. R. China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, P. R. China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Cao
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, P. R. China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, P. R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, P. R. China.
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Huang
authors.affiliation: Xinyang Central Hospital, Xinyang, P. R. China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Xinyang Central Hospital, Xinyang, P. R. China.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Ren
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E; D006046:Gold
keywords: Aptamer; Capillary electrophoresis; Chemiluminescence; Gold nanoparticles; Human immunoglobulin E