title: Genetic Variation in NFKB1 and NFKBIA and Susceptibility to Coronary Artery Disease in a Chinese Uygur Population.
abstract: OBJECTIVE
  Coronary artery disease (CAD) is the most common chronic inflammatory disease worldwide. NF-κB, a central regulator of inflammation, is involved in various inflammatory diseases. The aim of this study was to investigate the association between NFKB1 and NFKBIA polymorphisms and the susceptibility to CAD and their impact on plasma levels of IL-6 in a Chinese Uygur population.
  
  
  METHODS
  We genotyped NFKB1-94ins/del ATTG (rs28362491) and NFKBIA3' UTR A/G (rs696) using TaqMan SNP genotyping assays in 960 Uygur CAD cases and Uygur 1060 CAD-negative controls. IL-6 plasma levels were measured in 360 stable angina pectoris (SAP) cases and 360 controls using ELISA method.
  
  
  RESULTS
  There was no significant difference in the distribution of the genotypes and alleles of rs696 polymorphism in CAD cases and controls. Significant difference in the frequency of genotypes (P = 0.001) and alleles (P = 0.001) of rs28362491 polymorphism was observed in CAD cases compared to controls. In multivariate logistic regression analysis, SNP rs28362491 was consistently associated with CAD risk in a recessive model after adjustment for cardiovascular risk factors (OR = 1.581, 95% CI 1.222 to 2.046, P<0.001). SAP cases had significantly higher plasma levels of IL-6 compared to controls (P<0.001). General linear model analysis showed rs28362491 was independently associated with increased IL-6 levels by analyses of a recessive model (P<0.001) after adjustment for covariates.
  
  
  CONCLUSIONS
  Our study indicates that NFKB1-94 ins/del ATTG polymorphism may play a role in CAD susceptibility in Chinese Uygur population and is functionally associated with IL-6 expression, suggesting a mechanistic link between NFKB1-94 ins/del ATTG polymorphism and CAD susceptibility.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China; Department of Cardiology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Lai
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Li
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China.
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Yang
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China.
authors.forename: Yi-Tong
authors.initials: YT
authors.lastname: Ma
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: 1st Department of Cardiology, People's Hospital of Shaanxi Province, Xian, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhai
authors.affiliation: Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China; Clinical Research Institute of Xinjiang Medical University, Urumqi, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.affiliation: Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, China; Clinical Research Institute of Xinjiang Medical University, Urumqi, China.
authors.forename: Bang-Dang
authors.initials: BD
authors.lastname: Chen
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D015415:Biomarkers; D016022:Case-Control Studies; D002681:China; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics*; D054643:INDEL Mutation; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D052002:NF-kappa B p50 Subunit / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D020413:3' Untranslated Regions; D015415:Biomarkers; D020672:I-kappa B Proteins; D015850:Interleukin-6; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha