title: Derivation of Cinnamon Blocks Leukocyte Attachment by Interacting with Sialosides.
abstract: Molecules derived from cinnamon have demonstrated diverse pharmacological activities against infectious pathogens, diabetes and inflammatory diseases. This study aims to evaluate the effect of the cinnamon-derived molecule IND02 on the adhesion of leukocytes to host cells. The anti-inflammatory ability of IND02, a pentameric procyanidin type A polyphenol polymer isolated from cinnamon alcohol extract, was examined. Pretreatment with IND02 significantly reduced the attachment of THP-1 cells or neutrophils to TNF-α-activated HUVECs or E-selectin/ICAM-1, respectively. IND02 also reduced the binding of E-, L- and P-selectins with sialosides. Furthermore, IND02 could agglutinate human red blood cells (RBC), and the agglutination could be disrupted by sialylated glycoprotein. Our findings demonstrate that IND02, a cinnamon-derived compound, can interact with sialosides and block the binding of selectins and leukocytes with sialic acids.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Lin
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
authors.forename: Shih-Yun
authors.initials: SY
authors.lastname: Guu
authors.affiliation: Department of Pathology, Pingtung Christian Hospital, Pingtung 900, Taiwan.
authors.forename: Chan-Chuan
authors.initials: CC
authors.lastname: Tsai
authors.affiliation: Indus Biotech Private Limited, Kondhwa, Pune 411048, India.
authors.forename: Ekambaranellore
authors.initials: E
authors.lastname: Prakash
authors.affiliation: Indus Biotech Private Limited, Kondhwa, Pune 411048, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Viswaraman
authors.affiliation: Division of Colorectal Surgery, Department of Surgery, E-DA Hospital, Kaohsiung 82445, Taiwan.
authors.forename: Hsing-Bao
authors.initials: HB
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Center of Infectious Disease and Signaling Research, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Chang
mesh_terms: D000371:Agglutination / Q000187:drug effects; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D002935:Cinnamomum zeylanicum / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D019040:E-Selectin / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000378:metabolism; D044945:Proanthocyanidins / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D019040:E-Selectin; C000607183:IND02 polymer; D044945:Proanthocyanidins; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D019158:N-Acetylneuraminic Acid