title: [Comparative study on effects of blood enriching on mouse model of blood deficiency syndrome induced by cyclophosphamide of albiflorin, paeoniflorin on levels of GM-CSF, IL-3 and TNF-α].
abstract: OBJECTIVE
  To compare the effects and mechanism of blood enriching on mouse model of blood deficiency syndrome induced by cyclophosphamide of albiflorin and paeoniflorin.
  
  
  METHODS
  Albiflorin and paeoniflorin were determined by using animal models of blood deficiency syndrome induced by cyclophosphamide. The amount of WBC, RBC, HGB, index of thymus gland and spleen, and the changes of GM-CSF, IL-3 and TNF-α in serum were detected after the treatment.
  
  
  RESULTS
  Compared with the model group, the amount of WBC in the group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin were increased obviously (P < 0.01). The amount of RBC in the group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin were increased obviously (P < 0.01, P < 0.001), which did not had a significant difference compared with the same dose. The index of thymus gland in the group of 30 mg x kg(-1) albiflorin was superior to the model group (P < 0.01), the difference was significant compared with the same dose of paeoniflorin (P < 0.05). The GM-CSF in serum in all groups of 30 mg x kg(-1) albiflorin, 15 mg x kg(-1) albiflorin, 30 mg x kg(-1) paeoniflorin and 15 mg x kg(-1) paeoniflorin increased obviously (P < 0.01, P < 0.05, P < 0.01, P < 0.05); The IL-3 in serum in both group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin also increased (P < 0.001). The content of TNF-α in group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin were reduced (P < 0.01), which showed the obvious difference compared with the same dose group (P < 0.01).
  
  
  CONCLUSIONS
  Albiflorin had the effect of blood enriching by regulating the immune function, same with the paeoniflorin. The probable mechanism of nourishing blood and liver of Paeoniae Radix Alba was not only the better effect of adjusting the content of TNF-α, but also might act synergistically with paeoniflorin.
authors.forename: Ying-li
authors.initials: YL
authors.lastname: Zhu
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Jing-xia
authors.initials: JX
authors.lastname: Wang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Yang
authors.forename: Yin-feng
authors.initials: YF
authors.lastname: Huang
authors.forename: Sheng-sheng
authors.initials: SS
authors.lastname: Qu
authors.forename: Tian-tian
authors.initials: TT
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000187:drug effects; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000633:toxicity*; D005960:Glucosides / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood*; D006410:Hematopoiesis / Q000187:drug effects*; D007377:Interleukin-3 / Q000097:blood*; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D006027:Glycosides
chemical_list: D001952:Bridged-Ring Compounds; D003520:Cyclophosphamide; D005960:Glucosides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007377:Interleukin-3; D039821:Monoterpenes; D014409:Tumor Necrosis Factor-alpha; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin