title: Effect of tannic acid-fish scale gelatin hydrolysate hybrid nanoparticles on intestinal barrier function and α-amylase activity.
abstract: Practical application of tannic acid is limited because it readily binds proteins to form insoluble aggregates. In this study, tannic acid was self-assembled with fish scale gelatin hydrolysates (FSGH) to form stable colloidal complex nanoparticles. The nanoparticles prepared from 4 mg ml(-1) tannic acid and 4 mg ml(-1) FSGH had a mean particle size of 260.8 ± 3.6 nm, and showed a positive zeta potential (20.4 ± 0.4 mV). The nanoparticles acted as effective nano-biochelators and free radical scavengers because they provided a large number of adsorption sites for interaction with heavy metal ions and scavenging free radicals. The maximum adsorption capacity for Cu(2+) ions was 123.5 mg g(-1) and EC50 of DPPH radical scavenging activity was 21.6 ± 1.2 μg ml(-1). Hydroxyl radical scavenging effects of the nanoparticles were investigated by electron spin resonance spectroscopy. The copper-chelating capacity and free radical scavenging activity of the nanoparticles were associated with their capacity to inhibit Cu(2+) ion-induced barrier impairment and hyperpermeability of Caco-2 intestinal epithelial tight junction (TJ). However, α-amylase inhibitory activity of the nanoparticles was significantly lower than that of free tannic acid. The results suggest that the nanoparticles can ameliorate Cu(2+) ion induced intestinal epithelial TJ dysfunction without severely inhibiting the activity of the digestive enzymes.
authors.affiliation: Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243, Taiwan.
authors.forename: Shao-Jung
authors.initials: SJ
authors.lastname: Wu
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Ho
authors.forename: Shun-Zhou
authors.initials: SZ
authors.lastname: Jiang
authors.forename: Fwu-Long
authors.initials: FL
authors.lastname: Mi
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D003300:Copper / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D029941:Fish Proteins / Q000737:chemistry*; D005399:Fishes; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000201:enzymology*; D053758:Nanoparticles / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry*; D013634:Tannins / Q000737:chemistry* / Q000493:pharmacokinetics; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D029941:Fish Proteins; D011492:Protein Hydrolysates; D013634:Tannins; D003300:Copper; D005780:Gelatin; D000516:alpha-Amylases