title: LC-MS/MS measurement of ampicillin residue in swine tissues at 5 days after in-feed administration.
abstract: We assessed ampicillin (ABPC) concentrations of kidney, muscle and intestine after a 5-day withdrawal period in 2 male and a female young Large White pigs fed the diet containing ABPC (ABPC medicated feed, 24 mg/kg/day) for a week. The ABPC residues were measured with liquid chromatography-tandem mass spectrometry, and the mean recoveries and quantitation limits ranged from 91.8 to 97.2% and from 0.1 to 0.12 ng/g, respectively. The residual ABPC concentrations were ≤1.18 ng/g for the muscle, ≤0.53 ng/g for the kidney and ≤1.93 ng/g for the intestine, suggesting below the Japanese provisional maximum residue limits. These results reveal that the analytical method is developed for residual ABPC and that the withdrawal period is appropriate.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Kouko
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Mizuno
mesh_terms: D000667:Ampicillin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000821:Animal Feed; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods / Q000662:veterinary*; D004350:Drug Residues / Q000493:pharmacokinetics*; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000662:veterinary*
chemical_list: D000667:Ampicillin