title: KLF13 promotes porcine adipocyte differentiation through PPARγ activation.
abstract: BACKGROUND
  Adipogenesis is tightly controlled by a complex network of transcription factors acting at different stages of differentiation. Kruppel-like factors (KLFs) as a family of zinc-finger transcription factors play diverse roles during cell differentiation and development in mammals.
  
  
  RESULTS
  In the present study, we showed that KLF13 acts as a key regulator regulating porcine adipocyte differentiation. The expression of KLF13 was markedly up-regulated during the early stage of porcine adipocyte differentiation, which was followed by expression of PPARγ. Porcine adipocyte differentiation was significantly attenuated by the addition of siRNA against KLF13, whereas overexpression of KLF13 resulted in enhanced porcine adipocyte differentiation. Using promoter deletion and mutation analysis, we identified a KLF13-binding site within -593/-577 region of the porcine PPARγ proximal promoter, indicating that KLF13 directly interacts with porcine PPARγ promoter. However, inhibition of KLF13 by siRNA did not impair mouse adipocyte differentiation. In addition, knockdown and/or overexpression of KLF13 in 3 T3-L1 cells all did not influence expression of PPARγ2.
  
  
  CONCLUSIONS
  Collectively, our results suggest that KLF13 exist as a key pro-adipogenic transcription factor through transactivating PPARγ expression in porcine adipocyte differentiation, whereas no such effect was detected in mouse adipocyte differentiation.
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070 China.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Tongxing
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Swine Breeding and Genetics of the Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Hongshan, 430070 P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070 China.
authors.forename: Yuanfei
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070 China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070 China.|Key Laboratory of Swine Breeding and Genetics of the Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Hongshan, 430070 P. R. China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
keywords: Adipocyte differentiation; KLF13; Mouse; PPARγ; Porcine; Transcriptional regulation