title: Morphometric Examination of Mitochondrial Ultrastructure in Aging Cardiomyocytes.
abstract: Mitochondrial ultrastructure in cardiomyocytes from 3- and 24-month-old Wistar and OXYS rats was investigated using a new approach designed for morphometric analysis. The data fully confirm the electron microscopy data: the area of the inner mitochondrial membrane per unit volume of mitochondria was significantly decreased with age, as found on heart muscle section. In 3-month-old Wistar rats from the control group, this parameter was 41.3 ± 1.52 µm(2)/µm(3), whereas in OXYS rats it was decreased to 30.57 ± 1.74 µm(2)/µm(3). With age, an area of the inner mitochondrial membrane per unit volume of mitochondria declined in both rat strains: Wistar - from 41.3 ± 1.52 to 21.47 ± 1.22 µm(2)/µm(3), OXYS - from 30.57 ± 1.74 to 16.3 ± 0.89 µm(2)/µm(3). A new method that we designed and used for morphometric analysis notably simplifies the process of morphometric measurements and opens up good opportunities for its further optimization using image recognition technology.
authors.affiliation: Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, Moscow, 119991, Russia. fhb@belozersky.msu.ru.
authors.forename: Ch M
authors.initials: ChM
authors.lastname: El'darov
authors.forename: V B
authors.initials: VB
authors.lastname: Vays
authors.forename: I M
authors.initials: IM
authors.lastname: Vangeli
authors.forename: N G
authors.initials: NG
authors.lastname: Kolosova
authors.forename: L E
authors.initials: LE
authors.lastname: Bakeeva
mesh_terms: D000375:Aging / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D016922:Cellular Senescence; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism / Q000648:ultrastructure*; D051336:Mitochondrial Membranes / Q000378:metabolism / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar