title: [Assessment of the role of matrix metalloproteinase-3 gene polymorphism in the development of chronic heart failure].
abstract: OBJECTIVE
  To study the impact of a polymorphic variant of the matrix metalloproteinase-3 (MMP-3) gene on the development and course of chronic heart failure (CHF) in patients with coronary heart disease.
  
  
  METHODS
  A total of 277 patients with New York Heart Association (NYHA) Functional Class (FC) II-IV CHF were examined. MMP-3 -1171 5A/6A genetic polymorphism was studied by polymerase chain reaction. A control group included 136 patients (mean age 53.6 ± 4.8 years) with no signs of cardiovascular diseases, as evidenced by the examination.
  
  
  RESULTS
  The frequency of the 5A allele and the 5A/5A genotype of the 1171 5A/6A polymorphic locus in the MMP-3 gene proved to be higher in the patients with CHF than that in the control group. Thus, the variability of the 5A allele (odds ratio (OR), 1.39; 95% confidence interval (CI): 1.033 to 1.869; p = 0.03) and the 5A/5A genotype (OR, 2.15; 95% CI: 1.131 to 4.070; p = 0.02) was associated with increased risk for CHF. There were significant differences in the frequency of MMP-3 alleles and genotypes in relation to FC of CHF. The frequency of the 5A/5A genotype was substantially higher in the patients with NYHA FC IV CHF than that in those with NYHA FC II CHF (32.8% versus 15.2%; p = 0.039). The frequency of the 5A allele was significantly higher in the patients with NYHA FC IV CHF than that in those with NYHA FC II CHF (55.5% and 39.3%; respectively; p = 0.019). Thus, the carriage of the 5A allele and the 5A/5A genotype of the 1171 5A/6A polymorphic locus in the MMP-3 gene is a risk factor of severe CHF.
  
  
  CONCLUSIONS
  The determination of MMP-3 -1171 5A/6A polymorphism may be recommended for the early prediction of a risk for the development and severe course of CHF.
authors.affiliation: Department of Heart Failure, Research Institute of Cardiology, Tomsk, Russia.
authors.forename: A T
authors.initials: AT
authors.lastname: Teplyakov
authors.affiliation: Novosibirsk State Medical University, Ministry of Health of Russia, Novosibirsk, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Berezikova
authors.affiliation: Novosibirsk State Medical University, Ministry of Health of Russia, Novosibirsk, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Shilov
authors.affiliation: Department of Heart Failure, Research Institute of Cardiology, Tomsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Grakova
authors.affiliation: Department of Heart Failure, Research Institute of Cardiology, Tomsk, Russia.
authors.forename: Yu Yu
authors.initials: YY
authors.lastname: Torim
authors.affiliation: Novosibirsk State Medical University, Ministry of Health of Russia, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Efremov
authors.affiliation: Novosibirsk State Medical University, Ministry of Health of Russia, Novosibirsk, Russia.
authors.forename: I D
authors.initials: ID
authors.lastname: Safronov
authors.affiliation: Novosibirsk State Medical University, Ministry of Health of Russia, Novosibirsk, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Pustovetova
authors.affiliation: Department of Heart Failure, Research Institute of Cardiology, Tomsk, Russia.
authors.forename: R S
authors.initials: RS
authors.lastname: Karpov
mesh_terms: D000368:Aged; D000483:Alleles; D004247:DNA / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006333:Heart Failure / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3