title: Aging rather than sun exposure is a major determining factor for the density of miR-125b-positive epidermal stem cells in human skin.
abstract: Sunlight exposure and aging are two major factors in the deterioration of skin function. In the present study, we used eighty formalin-fixed human skin samples from sun-exposed and unexposed areas from old and young individuals to evaluate the presence of miR-125b-positive epidermal stem cells (ESCs) by in situ hybridization. miR-125b-positive ESCs were detected in the basal layer of the epidermis. The density of miR-125b-positive ESCs was significantly associated with age rather than sun exposure, whereas the density of miR-125b-positive ESCs tended to decrease in the sun-exposed area. These data suggest the potential use of miR-125b as a surrogate marker for the quality of epidermal cells.
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Shenqi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Kinki University School of Medicine, Osaka, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Hirao
authors.affiliation: Department of Dermatology, Kinki University School of Medicine, Osaka, Japan.
authors.forename: Tamae
authors.initials: T
authors.lastname: Wada
authors.affiliation: Kobe Technical Center, Procter & Gamble, Kobe, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Soh
authors.affiliation: Kobe Technical Center, Procter & Gamble, Kobe, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Toyama
authors.affiliation: Department of Dermatology, Kinki University School of Medicine, Osaka, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kawada
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D012867:Skin / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D013472:Sunlight; D055815:Young Adult
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs
keywords: UV; biomarker; epidermal stem cell; miRNA; skin aging