title: Protective effect of HO-1 transfection against ethanol-induced osteoblast damage.
abstract: Heme oxygenase-1 (HO-1) plays important roles in anti-oxidant, anti-inflammatory and immunoregulative activities. The aim of this study was to observe if HO-1 transfection could inhibit the damage of osteoblasts induced by ethanol. HO-1 was transfected into osteoblasts via constructed plasmid. After exposure to ethanol for 24 h, cytoactivity and apoptosis of osteoblasts were measured by MTT assay and flow cytometry, respectively. Furthermore, the oxidative stress and inflammatory factors in osteoblasts were measured. Compared to positive control group, the cytoactivity of transfected osteoblasts was significantly increased, and the apoptosis rate was significantly decreased (P<0.05). At the same time, the levels of reactive oxygen species (ROS), methane dicarboxylic aldehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) were significantly decreased (P<0.05), and superoxide dismutase (SOD) level was increased (P<0.05) in the transfected osteoblasts as compared with positive controls. These results suggest that HO-1 plays a protective role in osteoblasts, and HO-1 transfection can effectively inhibit bone damage induced by ethanol.
authors.affiliation: Department of Emergency Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, China. lijie3300@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Public Health, Xinxiang Medical University, Xinxiang, 453003, China.
authors.forename: Feng-Quan
authors.initials: FQ
authors.lastname: Zhang
authors.affiliation: Department of Emergency Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
authors.forename: Zhen-Ning
authors.initials: ZN
authors.lastname: Du
authors.affiliation: Department of Emergency Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Cai
authors.affiliation: Department of Emergency Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
authors.forename: Peng-Shan
authors.initials: PS
authors.lastname: Cai
authors.affiliation: Department of Emergency Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
mesh_terms: D002478:Cells, Cultured; D000431:Ethanol / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D014162:Transfection
chemical_list: D000431:Ethanol; D051547:Heme Oxygenase-1