title: A Novel Method for the Synthesis of (99m)Tc-Ofloxacin Kits Using D-Penicillamine as Coligand and Their Application as Infection Imaging Agent.
abstract: The employment of radiopharmaceuticals is increasing nowadays for infection imaging and early execution of patients having infectious or inflammatory complaints. The main aim of this study was to discover a novel method for the labeling of ofloxacin with (99m)Tc, optimization of labelling conditions to get higher percent yield, to assess kits radiochemical purity, in vitro stability, partition coefficient, protein binding, and intracellular accumulation in Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli in infected rabbits. Maximum labeling efficiency was achieved when 1.5 mg ofloxacin was labeled with 10-20 mCi sodium pertechnetate in the presence of 3 mg D-penicillamine, 75 μg SnCl₂. In vitro binding and biodistribution in Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli showed good results. This new complex is efficient for the imaging of infections caused by Gram-positive and Gram-negative bacteria.
authors.affiliation: Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Abdul Qadir
authors.affiliation: Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Shabnam
authors.identifier: 0000-0002-1786-5559
authors.initials: S
authors.lastname: Shahzad
authors.affiliation: Gujranwala Institute of Nuclear Medicine and Radiotherapy, Gujranwala, Pakistan.
authors.forename: Rashid
authors.initials: R
authors.lastname: Rasheed
authors.affiliation: Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Shahzad
authors.initials: S
authors.lastname: Anwar
authors.affiliation: Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Syeda
authors.initials: S
authors.lastname: Kiran Shahzadi
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007553:Isotope Labeling; D015242:Ofloxacin / Q000008:administration & dosage* / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000472:pathogenicity; D011817:Rabbits; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000737:chemistry*; D012485:Salmonella typhi / Q000302:isolation & purification / Q000472:pathogenicity; D013667:Technetium / Q000008:administration & dosage / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; D013667:Technetium; D015242:Ofloxacin