title: Transcriptional Profiling and Molecular Characterization of Astragalosides, Calycosin, and Calycosin-7-O-β-D-glucoside Biosynthesis in the Hairy Roots of Astragalus membranaceus in Response to Methyl Jasmonate.
abstract: We used the next-generation Illumina/Solexa HiSeq2000 platform on RNA analysis to investigate the transcriptome of Astragalus membranaceus hairy roots in response to 100 μM methyl jasmonate (MeJA). In total, 77,758,230 clean reads were assembled into 48,636 transcripts (average length of 1398 bp), which were clustered into 23,658 loci (genes). Of these, 19,940 genes were annotated by BLASTx searches. In addition, DESeq analysis showed that 2127 genes were up-regulated, while 1247 genes were down-regulated by MeJA. Seventeen novel astragaloside (AST) biosynthetic genes and seven novel calycosin and calycosin-7-O-β-D-glucoside (CG) biosynthetic genes were isolated. The accumulation of ASTs, calycosin, and CG increased significantly in MeJA-treated hairy roots compared with control hairy roots. Our findings will provide a valuable resource for molecular characterization of AST, calycosin, and CG biosynthetic pathways and may lead to new approaches to maximize their production and biomass productivity in the hairy roots of A. membranaceus.
authors.affiliation: †Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Pham Anh
authors.initials: PA
authors.lastname: Tuan
authors.affiliation: ‡Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Eunsook
authors.initials: E
authors.lastname: Chung
authors.affiliation: †Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thwe
authors.affiliation: †Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.affiliation: §Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Valan Arasu
authors.initials: VA
authors.lastname: Mariadhas
authors.affiliation: §Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Naif Abdullah
authors.initials: NA
authors.lastname: Al-Dhabi
authors.affiliation: ‡Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: †Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D027885:Astragalus propinquus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005960:Glucosides / Q000096:biosynthesis*; D007529:Isoflavones / Q000096:biosynthesis*; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005960:Glucosides; D007529:Isoflavones; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; C072239:methyl jasmonate
keywords: Astragalus membranaceus; astragaloside; calycosin; calycosin-7-O-β-d-glucoside; methyl jasmonate; transcriptional profiling