title: Arabidopsis RING E3 ubiquitin ligase AtATL80 is negatively involved in phosphate mobilization and cold stress response in sufficient phosphate growth conditions.
abstract: Phosphate (Pi) remobilization in plants is critical to continuous growth and development. AtATL80 is a plasma membrane (PM)-localized RING E3 ubiquitin (Ub) ligase that belongs to the Arabidopsis Tóxicos en Levadura (ATL) family. AtATL80 was upregulated by long-term low Pi (0-0.02 mM KH2PO4) conditions in Arabidopsis seedlings. AtATL80-overexpressing transgenic Arabidopsis plants (35S:AtATL80-sGFP) displayed increased phosphorus (P) accumulation in the shoots and lower biomass, as well as reduced P-utilization efficiency (PUE) under high Pi (1 mM KH2PO4) conditions compared to wild-type plants. The loss-of-function atatl80 mutant line exhibited opposite phenotypic traits. The atatl80 mutant line bolted earlier than wild-type plants, whereas AtATL80-overexpressors bloomed significantly later and produced lower seed yields than wild-type plants under high Pi conditions. Thus, AtATL80 is negatively correlated not only with P content and PUE, but also with biomass and seed yield in Arabidopsis. In addition, AtATL80-overexpressors were significantly more sensitive to cold stress than wild-type plants, while the atatl80 mutant line exhibited an increased tolerance to cold stress. Taken together, our results suggest that AtATL80, a PM-localized ATL-type RING E3 Ub ligase, participates in the Pi mobilization and cold stress response as a negative factor in Arabidopsis.
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Suh
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea. Electronic address: wtkim@yonsei.ac.kr.
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D018533:Biomass; D002462:Cell Membrane / Q000201:enzymology; D003080:Cold Temperature; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013312:Stress, Physiological; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D010710:Phosphates; D010758:Phosphorus; D044767:Ubiquitin-Protein Ligases
keywords: Arabidopsis thaliana; Cold stress response; Phosphate mobilization; Phosphorus utilization efficiency; RING E3 Ub ligase; Seed yield