title: Gene expression profiling of changes induced by maternal diabetes in the embryonic heart.
abstract: Cardiovascular defects are one of the most common congenital defects associated with maternal diabetes. Based on whole embryo gene expression microarray analysis, 11 genes were chosen for temporal expression analysis of diabetes-exposed hearts. The majority of the selected genes were deregulated in diabetes-exposed hearts compared to our controls at E13.5, E14.5, and E18.5. We showed increased hypoxia and HIF-1α protein levels in diabetes-exposed hearts at E10.5, which is a critical time point for the induction of developmental defects associated with diabetic embryopathy. Additionally, we found increased cardiac Vegfa levels that might trigger developmental abnormalities associated with diabetic embryopathy. Our results show that maternal diabetes affects the temporal expression pattern of gene encoding molecules involved in heart development and tissue remodelling and that these molecules might affect heart maturation processes and thus, the final outcome of diabetic pregnancies.
authors.affiliation: Institute of Biotechnology AS CR, Prague, Czechia.
authors.forename: Romana
authors.initials: R
authors.lastname: Bohuslavova
authors.affiliation: Institute of Biotechnology AS CR, Prague, Czechia.
authors.forename: Lada
authors.initials: L
authors.lastname: Skvorova
authors.affiliation: Institute of Biotechnology AS CR, Prague, Czechia.
authors.forename: Radka
authors.initials: R
authors.lastname: Cerychova
authors.affiliation: Institute of Biotechnology AS CR, Prague, Czechia. Electronic address: gpavlinkova@ibt.cas.cz.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pavlinkova
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000378:metabolism; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse
keywords: Cardiovascular defects; Diabetic embryopathy; Gene expression; Vascular endothelial growth factor A (VEGF-A)