title: New candidate genes for the fine regulation of the colour of grapes.
abstract: In the last decade, great progress has been made in clarifying the main determinants of anthocyanin accumulation in grape berry skin. However, the molecular details of the fine variation among cultivars, which ultimately contributes to wine typicity, are still not completely understood. To shed light on this issue, the grapes of 170 F1 progeny from the cross 'Syrah'×'Pinot Noir' were characterized at the mature stage for the content of 15 anthocyanins during four growing seasons. This huge data set was used in combination with a dense genetic map to detect genomic regions controlling the anthocyanin pathway both at key enzymatic points and at particular branches. Genes putatively involved in fine tuning the global regulation of anthocyanin biosynthesis were identified by exploring the gene predictions in the QTL (quantitative trait locus) confidence intervals and their expression profile during berry development in offspring with contrasting anthocyanin accumulation. New information on some aspects which had scarcely been investigated so far, such as anthocyanin transport into the vacuole, or completely neglected, such as acylation, is provided. These genes represent a valuable resource in grapevine molecular-based breeding programmes to improve both fruit and wine quality and to tailor wine sensory properties according to consumer demand.
authors.affiliation: Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy laura.costantini@fmach.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Costantini
authors.affiliation: Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Malacarne
authors.affiliation: Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lorenzi
authors.affiliation: Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Troggio
authors.affiliation: Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Mattivi
authors.affiliation: Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Moser
authors.affiliation: Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy.
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Grando
mesh_terms: D000872:Anthocyanins / Q000235:genetics / Q000378:metabolism*; D005638:Fruit / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010858:Pigmentation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins
keywords: Anthocyanin; Vitis vinifera.; berry; candidate gene; metabolic profiling; microarray; quantitative trait locus