title: scFv from Antibody That Mimics gp43 Modulates the Cellular and Humoral Immune Responses during Experimental Paracoccidioidomycosis.
abstract: Paracoccidioidomycosis (PCM), caused by Paracoccidioides species is a prevalent systemic and progressive mycosis that occurs in Latin America. It is caused by Paracoccidioides species. Immunization with dendritic cells transfected with a plasmid encoding the scFv (pMAC/PS-scFv) that mimics the main antigen of P. brasiliensis (gp43) confers protection in experimental PCM. DCs link innate and adaptive immunity by recognizing invading pathogens and selecting the type of effector T cell to mediate the immune response. Here, we showed that DC-pMAC/PS-scFv induces the activation of CD4+ and CD8+ T cells. Moreover, our results demonstrated that BALB/c mice infected with P. brasiliensis and treated with DC-pMAC/PS-scFv showed the induction of specific IgG production against gp43 and IFN-γ, IL-12 and IL-4 cytokines. Analysis of regional lymph nodes revealed increases in the expression of clec7a, myd88, tlr2, gata3 and tbx21, which are involved in the immune response. Taken together, our results indicate that the scFv modulates the humoral and cellular immune responses and presents epitopes to CD4+ and CD8+ T cells.
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Grasielle Pereira
authors.initials: GP
authors.lastname: Jannuzzi
authors.affiliation: Instituto de Biologia, Universidade de Brasília, Brasília, Brazil.
authors.forename: Aldo Henrique F P
authors.initials: AH
authors.lastname: Tavares
authors.affiliation: Departamento de Química, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Gilberto Hideo
authors.initials: GH
authors.lastname: Kaihami
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: José Roberto Fogaça
authors.initials: JR
authors.lastname: de Almeida
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Sandro Rogério
authors.initials: SR
authors.lastname: de Almeida
authors.affiliation: Departamento de Ciências Biológicas do Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, Brazil.
authors.forename: Karen Spadari
authors.initials: KS
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D005656:Fungal Proteins / Q000276:immunology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization; D016219:Immunotherapy, Adoptive; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D051379:Mice; D010229:Paracoccidioidomycosis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000628:therapy; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014162:Transfection
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D005656:Fungal Proteins; D006023:Glycoproteins; D057127:Single-Chain Antibodies