title: Impact of chlorpyrifos on health biomarkers of broiler chicks.
abstract: The present study aimed to investigate the deleterious effects of chlorpyrifos (CPF) in experimentally exposed broiler birds. The experiment was carried out on one day old (n = 120) broiler chicks. The CPF was reconstituted in corn oil as vehicle (1 ml/kg) to obtain a final concentration of a single dose to the birds 5, 10 and 20 mg/kg body weight (BW) for fourteen days of the experiment through the stomach tube. The control group was given corn oil 1 ml/kg only. Birds exposed to high dose (20 mg/kg BW) showed signs of toxicity (salivation, lacrimation, gasping, convulsions, frequent defecation and tremors). The birds exposed to 10 and 20 mg/kg showed significantly (P ≤ 0.05) decreased body weight. Significantly (P ≤ 0.05) decreased hematological parameters i.e. total erythrocyte counts, hemoglobin concentration, hematocrit and total leukocyte were observed in the high dosed group as compared to control and other low dosed fed birds. Serum protein and albumin showed a significant (P ≤ 0.05) increase in high dosed CPF fed birds. Non significant results were observed in the case of globulin. The acetylcholinestrease (AChE) activity was significantly (P ≤ 0.05) decreased in blood, serum and plasma in CPF fed birds compared to control birds. In CPF fed birds as compared to control birds we found significantly (P ≤ 0.05) higher levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Necrotic and degenerative changes were observed on histopathological investigations of spleen, kidneys, bursa of Fabricius, thymus and brain tissues in CPF exposed birds. In conclusion the chlorpyrifos induced toxicopathological effects on health biomarkers of broiler chicks.
authors.affiliation: Department of Pathology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad Zishan
authors.initials: MZ
authors.lastname: Ahmad
authors.affiliation: Department of Pathology, University of Agriculture, Faisalabad, Pakistan. Electronic address: ahrar1122@uaf.edu.pk.
authors.forename: Ahrar
authors.initials: A
authors.lastname: Khan
authors.affiliation: Department of Pathology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: M Tariq
authors.initials: MT
authors.lastname: Javed
authors.affiliation: Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Hussain
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000473:pathology; D002645:Chickens / Q000378:metabolism*; D004390:Chlorpyrifos / Q000633:toxicity*; D002802:Cholinesterases / Q000378:metabolism; D004906:Erythrocyte Count; D006400:Hematocrit; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D013154:Spleen / Q000187:drug effects / Q000473:pathology
chemical_list: D015415:Biomarkers; D002802:Cholinesterases; D004390:Chlorpyrifos
keywords: Broiler chicks; Chlorpyrifos; Health biomarkers