title: Stimulus-Responsive, Biodegradable, Biocompatible, Covalently Cross-Linked Hydrogel Based on Dextrin and Poly(N-isopropylacrylamide) for in Vitro/in Vivo Controlled Drug Release.
abstract: A novel stimulus-sensitive covalently cross-linked hydrogel derived from dextrin, N-isopropylacrylamide, and N,N'-methylene bis(acrylamide) (c-Dxt/pNIPAm), has been synthesized via Michael type addition reaction for controlled drug release application. The chemical structure of c-Dxt/pNIPAm has been confirmed through Fourier transform infrared (FTIR) spectroscopy and (1)H and (13)C NMR spectral analyses. The surface morphology of the hydrogel has been studied by field emission scanning electron microscopic (FE-SEM) and environmental scanning electron microscopic (E-SEM) analyses. The stimulus responsiveness of the hydrogel was studied through equilibrium swelling in various pH media at 25 and 37 °C. Rheological study was performed to measure the gel strength and gelation time. Noncytotoxicity of c-Dxt/pNIPAm hydrogel has been studied using human mesenchymal stem cells (hMSCs). The biodegradability of c-Dxt/pNIPAm was confirmed using hen egg lysozyme. The in vitro and in vivo release studies of ornidazole and ciprofloxacin imply that c-Dxt/pNIPAm delivers both drugs in a controlled way and would be an excellent alternative for a dual drug carrier. The FTIR, powder X-ray diffraction (XRD), and UV-vis-near infrared (NIR) spectra along with the computational study predict that the drugs remain in the matrix through physical interaction. A stability study signifies that the drugs (ornidazole ∼97% and ciprofloxacin ∼98%) are stable in the tablet formulations for up to 3 months.
authors.affiliation: †Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian School of Mines, Dhanbad 826004, India.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Das
authors.forename: Paulomi
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: §Departmental of Pharmaceutical Sciences, Birla Institutes of Technology, Mesra, Ranchi, Jharkhand 835215, India.
authors.forename: Animesh
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: †Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian School of Mines, Dhanbad 826004, India.
authors.forename: Chanchal
authors.initials: C
authors.lastname: Haldar
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
authors.affiliation: ∥Discipline of Inorganic Materials and Catalysis, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat 364002, India.
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Panda
authors.affiliation: †Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian School of Mines, Dhanbad 826004, India.
authors.forename: Sagar
authors.initials: S
authors.lastname: Pal
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000493:pharmacokinetics; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002939:Ciprofloxacin / Q000737:chemistry / Q000493:pharmacokinetics; D003692:Delayed-Action Preparations; D003912:Dextrins / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D059630:Mesenchymal Stem Cells; D009950:Ornidazole / Q000737:chemistry / Q000493:pharmacokinetics; D011817:Rabbits; D023822:Tissue Engineering
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D003912:Dextrins; D004337:Drug Carriers; C052970:poly-N-isopropylacrylamide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002939:Ciprofloxacin; D009950:Ornidazole
keywords: biodegradable; controlled drug release; dextrin; hydrogel; noncytotoxicity