title: First report of Enterocytozoon bieneusi and Encephalitozoon intestinalis infection of wild mice in Slovakia.
abstract: Increased risk of zoonotic transmission of the potential human pathogenic species Enterocytozoon bieneusi, Encephalitozoon intestinalis and Encephalitozoon cuniculi was detected in wild immunocompetent mice (Mus musculus musculus; n=280). Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, E. bieneusi was detected in 3 samples (1.07 %), E. cuniculi in 1 sample (0.35 %) and E. intestinalis in 1 sample (0.35 %). The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.
authors.affiliation: Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
authors.forename: Oľga
authors.initials: O
authors.lastname: Danišová
authors.affiliation: Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Valenčáková
authors.affiliation: Department of Zoology, Slovak Academy of Science, Košice, Slovak Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Stanko
authors.affiliation: Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Luptáková
authors.affiliation: Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
authors.forename: Antónia
authors.initials: A
authors.lastname: Hasajová
mesh_terms: D000818:Animals; D016819:Encephalitozoon / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016890:Encephalitozoonosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D051379:Mice; D016881:Microsporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012376:Rodent Diseases / Q000453:epidemiology* / Q000469:parasitology; D012377:Rodentia; D018154:Slovakia / Q000453:epidemiology