title: A rapid method for chemical fingerprint analysis of Pan Panax notoginseng powders by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
abstract: A method coupling ultra-performance liquid chromatography (UPLC) with quadrupole time-of-flight mass spectrometer (Qtof MS) using the electrospray ionization (ESI) source was developed for the identification of the major saponins from Panax notoginseng powder (PNP). Ten different PNP samples were analyzed and evaluated for their quality by similarity evaluation and principle component analysis (PCA). Based on the accurate mass, summarized characteristic fragmentation behaviors, retention times of different types of saponins, related botanical biogenesis, and reported chromatographic behavior of saponins, fifty-one common peaks were effectively separated and identified, including 28 protopanaxadiol saponins and 18 protopanaxatriol saponins. Simultaneously, 15 significant discrepancy compounds were identified from the disqualified PNP samples. The established UPLC/Qtof MS fingerprint method was successfully applied for profiling and identifying the major saponins of PNP, providing a fast quality evaluation tool for distinguishing the authentic PNP and the adulterated products.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: He-Shuil
authors.initials: HS
authors.lastname: Yu
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xin-Bo
authors.initials: XB
authors.lastname: Song
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Kang
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Liu
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Man
authors.initials: M
authors.lastname: Cao
authors.affiliation: Waters Corporation, Milford, MA 01757, USA.
authors.forename: Kate
authors.initials: K
authors.lastname: Yu
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.
authors.forename: Ting-Guo
authors.initials: TG
authors.lastname: Kang
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China. Electronic address: mabaiping@sina.com.
authors.forename: Bai-Ping
authors.initials: BP
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D053723:Panax notoginseng / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D011208:Powders
keywords: Chemical fingerprint; Ginsenosides; Notoginsenosides; Panax notoginseng powder; UPLC/Qtof MS(E)