title: Targeting eradication of chronic myeloid leukemia using chimeric oncolytic adenovirus to drive IL-24 expression.
abstract: Chronic myeloid leukemia (CML) is a clonal disorder in which cells of the myeloid lineage undergo massive clonal expansion as well as resistance to conventional chemotherapy. Gene therapy hold a great promise for treatment of malignancies based on the transfer of genetic material to the tissues. In this study, we explore whether chimeric oncolytic adenovirus-mediated transfer of human interleukin-24 (IL-24) gene induce the enhanced antitumor potency. Our results showed that chimeric oncolytic adenovirus carrying hIL-24 (AdCN205-11-IL-24) could produce high levels of hIL-24 in CML cancer cells, as compared with constructed double-regulated oncolytic adenovirus expressing hIL-24 (AdCN205-IL-24). AdCN205-11-IL-24 could specifically induce cytotoxocity to CML cancer cells, but little or no effect on normal cell lines. AdCN205-11-IL-24 exhibited remarkable anti-tumor activities and induce higher antitumor activity to CML cancer cells by inducing apoptosis in vitro. Our study may provides a potent and safe tool for CML gene therapy.
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine Zhejiang 310058, Hangzhou ; School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Xubin
authors.initials: X
authors.lastname: Wei
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Xupang
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University Hangzhou 310018, Zhejiang, P. R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine Zhejiang 310058, Hangzhou.
authors.forename: Jimin
authors.initials: J
authors.lastname: Shao
mesh_terms: D000256:Adenoviridae; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D000099180:Interleukin-24
chemical_list: D007378:Interleukins; D000099180:Interleukin-24
keywords: Chimeric oncolytic adenovirus; IL-24 gene; chronic myeloid leukemia; fiber; gene-therapy