title: [Determination of oleanic acid and paeoniflorin in Paeonia lactiflora by ultrasound-assisted ionic liquid-reversed phase liquid chromatography].
abstract: Four kinds of ionic liquids [BMIM] Br, [BMIM] BF4, [BMIM] PF6, [HMIM] PF6 were used to analyze the content of oleanic acid and paeoniflorin in Paeonia lactiflora with ultrasonic-assisted extraction coupled with HPLC. The chromatographic column, Purospher star RP-C18 (4.6 mm x 250 mm, 5 μm), was used. Acetonitrile and water (90:10) as mobile phase was used to determine the content of oleanic acid with a gradient elution and flow rate at 1.00 mL · min(-1), detection wavelength at 210 nm, chromatographic column temperature at room temperature. Paeoniflorin content was determined using acetonitrile and water (18:82) as mobile phase with a gradient elution and flow rate at 1.00 mL · min(-1), detection wavelength at 250 nm, the chromatographic column temperature at room temperature. The result show that oleanic acid has the highest extraction yield when the conditions are solid-liquid ratio of 1:80 (g · mL(-1)), and the [BMIM] Br methanol solution concentration of 0.6 mol · L(-1). Under the optimal extraction conditions, the content of oleanic acid from 0.24 to 3.76 μg showed a good linearity (r = 0.9999), the average recovery was 97.20%. Paeoniflorin has the highest extraction yield when the conditions are solid-liquid ratio of 1:130 (g · mL(-1)), and the [C4 MIM] PF6 methanol solution concentration of 0.6 mol · L(-1). Under the optimal extraction conditions, paeoniflorin content from 0.42 to 4.20 μg showed a good lin- earity (r = 1.000), the average recovery was 98.84%. This method is simple and reliable, its repeatability is also very good. It has important significance in the study P. lactiflora of ionic liquid microextraction.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Dong-dong
authors.initials: DD
authors.lastname: Li
authors.forename: Hong-shuai
authors.initials: HS
authors.lastname: Yang
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Chen
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Wei
authors.forename: Wen-yi
authors.initials: WY
authors.lastname: Kang
authors.forename: Xiu-chun
authors.initials: XC
authors.lastname: Guo
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D052578:Ionic Liquids / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis*; D009828:Oleanolic Acid / Q000032:analysis*; D029596:Paeonia / Q000737:chemistry*; D014465:Ultrasonics; D006027:Glycosides
chemical_list: D005960:Glucosides; D052578:Ionic Liquids; D039821:Monoterpenes; D009828:Oleanolic Acid; C015423:peoniflorin; D006027:Glycosides