title: Identification of Biomarkers Associated With Alzheimer's Disease by Bioinformatics Analysis.
abstract: BACKGROUND
  This study aimed to explore the biomarkers of Alzheimer's disease (AD).
  
  
  METHODS
  The microarray data of GSE16759 were from the expression profile samples of 4 parietal lobe tissues from patients with AD and 4 ones from age-matched control participants. The differentially expressed micro RNAs (miRNAs) and genes (DEGs) underwent hierarchical clustering and function analysis followed by target genes prediction. Finally, DEGs were mapped to the target genes to construct miRNA-regulated networks.
  
  
  RESULTS
  A total of 427 DEGs were obtained and clustered into 5 functions. After DEGs were mapped to the predicted target genes, 313 regulatory pairs were established. The target genes SEC22 vesicle trafficking protein homolog B (SEC22B) and SEC63 homolog (SEC63) regulated by miRNA-206, RAB10, member RAS oncogene family (RAB10) regulated by miRNA-655, and fms-related tyrosine kinase 1 (FLT1) regulated by miRNA-30e-3p and miRNA-369-3p were involved in the biological processes of protein transport and regulation of cell motion.
  
  
  CONCLUSIONS
  The target genes SEC22B, RAB10, and FLT1 may be potential biomarkers of AD.
authors.affiliation: Department of Neurology, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.affiliation: Department of Neurology, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Sheng
authors.affiliation: Department of Neurology, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, China xiaohonglixhl@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics*; D015415:Biomarkers; D019295:Computational Biology / Q000379:methods*; D053263:Gene Regulatory Networks; D005819:Genetic Markers; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics; D018832:Molecular Chaperones; D010296:Parietal Lobe; D050683:R-SNARE Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D020691:rab GTP-Binding Proteins / Q000235:genetics*
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D008565:Membrane Proteins; D035683:MicroRNAs; D018832:Molecular Chaperones; D050683:R-SNARE Proteins; D016601:RNA-Binding Proteins; C487464:SEC63 protein, human; C572028:Sec22B protein, human; C538901:Rab10 protein, human; D020691:rab GTP-Binding Proteins
keywords: Alzheimer’s disease; differentially expressed genes; functional cluster; micro RNA; regulatory network; target genes