title: The effectively specific recognition of bovine serum albumin imprinted silica nanoparticles by utilizing a macromolecularly functional monomer to stabilize and imprint template.
abstract: Structural stability of the template is one of the most important considerations during the preparation of protein imprinting technology. To address this limitation, we propose a novel and versatile strategy of utilizing macromolecularly functional monomers to imprint biomacromolecules. Results from circular dichroism and synchronous fluorescence experiments reflect the macromolecularly functional monomers tendency to interact with the protein surface instead of permeating it and destroying the hydrogen bonds that maintain the protein's structural stability, therefore stabilizing the template protein structure during the preparation of imprinted polymers. The imprinted polymers composed of macromolecularly functional monomers or their equivalent micromolecularly functional monomers over silica nanoparticles were characterized and carried out in batch rebinding test and competitive adsorption experiments. In batch rebinding test, the imprinted particles prepared with macromolecularly functional monomers exhibited an imprinting factor of 5.8 compared to those prepared by micromolecularly functional monomers with the imprinting factor of 3.4. The selective and competitive adsorption experiments also demonstrated the imprinted particles made by macromolecularly functional monomers possessed much better selectivity and specific recognition ability for template protein. Therefore, using macromolecularly functional monomers to imprint may overcome the mutability of biomacromolecule typically observed during the preparation of imprinted polymers, and thus promote the further development of imprinting technology.
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China. Electronic address: qianliwei@mail.nwpu.edu.cn.
authors.forename: Liwei
authors.initials: L
authors.lastname: Qian
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guan
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Du
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Renyuan
authors.initials: R
authors.lastname: Song
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Chaoli
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, The Key Laboratory of Space Applied Physics and Chemistry, Xi'an 710072, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Song
mesh_terms: D000327:Adsorption; D002942:Circular Dichroism; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide
keywords: Molecular recognition; Protein imprinted nanoparticles; Protein stabilizing; Surface imprinting