title: Effects of T-2 toxin on the regulation of steroidogenesis in mouse Leydig cells.
abstract: T-2 toxin is one of the mycotoxins, a group of type A trichothecenes produced by several fungal genera including Fusarium species, which may lead to the decrease of testosterone secretion in primary Leydig cells derived from mouse testis. The previous study demonstrated T-2 toxin decrease the testosterone biosynthesis in the primary Leydig cells derived from the mouse testis directly. In this study, we further examined the direct biological effects of T-2 toxin on the process of steroidogenesis, primarily in Leydig cells of mice. Leydig cells of mature mouse were purified by Percoll gradient centrifugation and the cell purity was determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining. To examine the decrease in T-2 toxin-induced testosterone secretion, we measured the transcription level of three key steroidogenic enzymes including 3β-HSD-1, cytochrome P450 side-chain cleavage (P450scc) enzyme, and steroidogenic acute regulatory (StAR) protein in T-2 toxin/human chorionic gonadotropin (hCG) co-treated cells. Our previous study showed that T-2 toxin (10(-7), 10(-8), and 10(-9) M) significantly suppressed hCG (10 ng/ml)-induced testosterone secretion. The studies demonstrated that the suppressive effect is correlated with a decrease in the level of transcription of 3β-HSD-1, P450scc, and StAR (p < 0.05).
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, China Laboratory of Animal Nutrition and Health and Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, China.
authors.forename: Jian Ying
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, China College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, China zyf_1001@haust.edu.cn.
authors.forename: Yong Fa
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
authors.forename: Yuan Xiao
authors.initials: YX
authors.lastname: Li
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, China College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China.
authors.forename: Gui Ping
authors.initials: GP
authors.lastname: Guan
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, China.
authors.forename: Xiang Feng
authors.initials: XF
authors.lastname: Kong
authors.affiliation: Biotechnology Research Center of Southwest University, Chong Qing, China.
authors.forename: Ai Min
authors.initials: AM
authors.lastname: Liang
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, China.
authors.forename: Kai Wang
authors.initials: KW
authors.lastname: Ma
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Da Li
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, China.
authors.forename: Xue Fei
authors.initials: XF
authors.lastname: Bai
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002498:Centrifugation; D007985:Leydig Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013605:T-2 Toxin / Q000633:toxicity*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D013605:T-2 Toxin
keywords: 3β-HSD-1; P450scc; StAR; T-2 toxin; mouse Leydig cells