title: The comparative advantages of ethanol and sucrose as co-substrates in the degradation of an anionic surfactant: microbial community selection.
abstract: The efficiency of linear alkylbenzene sulfonate (LAS) removal from laundry wastewater and the related microbial community was investigated in an anaerobic fluidized bed reactor (AFBR). The AFBR was operated in three stages, in addition to the biomass adaptation stage without LAS (stage I). The stages were differentiated by their supplementary co-substrates: stage II had sucrose plus ethanol, stage III had only ethanol, and stage IV had no co-substrate. The replacement of sucrose plus ethanol with ethanol only for the substrate composition favored the efficiency of LAS removal, which remained high after the co-substrate was removed (stage II: 52 %; stage III: 73 %; stage IV: 77 %). A transition in the microbial community from Comamonadaceae to Rhodocyclaceae in conjunction with the co-substrate variation was observed using ion sequencing analysis. The microbial community that developed in response to an ethanol-only co-substrate improved LAS degradation more than the community that developed in response to a mixture of sucrose and ethanol, suggesting that ethanol is a better option for enriching an LAS-degrading microbial community.
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. Trabalhador Sancarlense, 400, 13566-590, São Carlos, SP, Brazil. thais.macedo@usp.br.
authors.forename: T Z
authors.initials: TZ
authors.lastname: Macedo
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. Trabalhador Sancarlense, 400, 13566-590, São Carlos, SP, Brazil.
authors.forename: D Y
authors.initials: DY
authors.lastname: Okada
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. Trabalhador Sancarlense, 400, 13566-590, São Carlos, SP, Brazil.
authors.forename: T P
authors.initials: TP
authors.lastname: Delforno
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. Trabalhador Sancarlense, 400, 13566-590, São Carlos, SP, Brazil.
authors.forename: J K
authors.initials: JK
authors.lastname: Braga
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luiz, Km 235, SP 310, 13565-905, São Carlos, SP, Brazil.
authors.forename: E L
authors.initials: EL
authors.lastname: Silva
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. Trabalhador Sancarlense, 400, 13566-590, São Carlos, SP, Brazil. varesche@sc.usp.br.
authors.forename: M B A
authors.initials: MB
authors.lastname: Varesche
mesh_terms: D017738:Alkanesulfonic Acids / Q000302:isolation & purification / Q000378:metabolism; D000838:Anions; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D000431:Ethanol / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism*; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods
chemical_list: D017738:Alkanesulfonic Acids; D000838:Anions; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C030641:alkylbenzyl sulfonic acid; D000431:Ethanol; D013395:Sucrose
keywords: Carbon source; Co-metabolism; Curvibacter; Dechloromonas; Ion sequencing; Linear alkylbenzene sulfonate (LAS)