title: 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: A National Resource for Ultrahigh Resolution Mass Analysis.
abstract: We describe the design and initial performance of the first 21 tesla Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The 21 tesla magnet is the highest field superconducting magnet ever used for FT-ICR and features high spatial homogeneity, high temporal stability, and negligible liquid helium consumption. The instrument includes a commercial dual linear quadrupole trap front end that features high sensitivity, precise control of trapped ion number, and collisional and electron transfer dissociation. A third linear quadrupole trap offers high ion capacity and ejection efficiency, and rf quadrupole ion injection optics deliver ions to a novel dynamically harmonized ICR cell. Mass resolving power of 150,000 (m/Δm(50%)) is achieved for bovine serum albumin (66 kDa) for a 0.38 s detection period, and greater than 2,000,000 resolving power is achieved for a 12 s detection period. Externally calibrated broadband mass measurement accuracy is typically less than 150 ppb rms, with resolving power greater than 300,000 at m/z 400 for a 0.76 s detection period. Combined analysis of electron transfer and collisional dissociation spectra results in 68% sequence coverage for carbonic anhydrase. The instrument is part of the NSF High-Field FT-ICR User Facility and is available free of charge to qualified users.
authors.affiliation: National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, FL, 32310, USA, hendrick@magnet.fsu.edu.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Hendrickson
authors.forename: John P
authors.initials: JP
authors.lastname: Quinn
authors.forename: Nathan K
authors.initials: NK
authors.lastname: Kaiser
authors.forename: Donald F
authors.initials: DF
authors.lastname: Smith
authors.forename: Greg T
authors.initials: GT
authors.lastname: Blakney
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Alan G
authors.initials: AG
authors.lastname: Marshall
authors.forename: Chad R
authors.initials: CR
authors.lastname: Weisbrod
authors.forename: Steven C
authors.initials: SC
authors.lastname: Beu