title: Characterization of antiproliferative potential and biological targets of a copper compound containing 4'-phenyl terpyridine.
abstract: Several copper complexes have been assessed as anti-tumor agents against cancer cells. In this work, a copper compound [Cu(H2O){OS(CH3)2}L](NO3)2 incorporating the ligand 4'-phenyl-terpyridine antiproliferative activity against human colorectal, hepatocellular carcinomas and breast adenocarcinoma cell lines was determined, demonstrating high cytotoxicity. The compound is able to induce apoptosis and a slight delay in cancer cell cycle progression, probably by its interaction with DNA and induction of double-strand pDNA cleavage, which is enhanced by oxidative mechanisms. Moreover, proteomic studies indicate that the compound induces alterations in proteins involved in cytoskeleton maintenance, cell cycle progression and apoptosis, corroborating its antiproliferative potential.
authors.affiliation: Departamento de Ciências da Vida, UCIBIO, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, Campus de Caparica, 2829-516, Caparica, Portugal.
authors.forename: Ana Soraia
authors.initials: AS
authors.lastname: Mendo
authors.forename: Sara
authors.initials: S
authors.lastname: Figueiredo
authors.forename: Catarina
authors.initials: C
authors.lastname: Roma-Rodrigues
authors.forename: Paula A
authors.initials: PA
authors.lastname: Videira
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ma
authors.forename: Mário
authors.initials: M
authors.lastname: Diniz
authors.forename: Miguel
authors.initials: M
authors.lastname: Larguinho
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Costa
authors.forename: João C
authors.initials: JC
authors.lastname: Lima
authors.forename: Armando J L
authors.initials: AJ
authors.lastname: Pombeiro
authors.forename: Pedro V
authors.initials: PV
authors.lastname: Baptista
authors.forename: Alexandra R
authors.initials: AR
authors.lastname: Fernandes
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry; D004847:Epithelial Cells / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005434:Flow Cytometry; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D018384:Oxidative Stress; D040901:Proteomics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D011725:Pyridines / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; C000595707:BCL2 protein, human; D056831:Coordination Complexes; D019253:Proto-Oncogene Proteins c-bcl-2; D011725:Pyridines; D012709:Serum Albumin; D051028:bcl-2-Associated X Protein; D003300:Copper; D004247:DNA; C505407:CASP3 protein, human; D053148:Caspase 3