title: The Neuroprotective Effects of Decursin Isolated from Angelica gigas Nakai Against Amyloid β-Protein-Induced Apoptosis in PC 12 Cells via a Mitochondria-Related Caspase Pathway.
abstract: Decursin, purified from Angelica gigas Nakai, has been proven to exert neuroprotective property. Previous study revealed decursin protected the PC12 cells from Aβ25-35-induced oxidative cytotoxicity. The present study aimed to investigate whether decursin could protect PC12 cells from apoptosis caused by Aβ. Our results indicated that pretreatment of PC12 cells with decursin significantly inhibited Aβ25-35-induced cytotoxicity and apoptosis. The mechanism of action is likely to reverse Aβ25-35-induced mitochondrial dysfunction, including the reduction of mitochondrial membrane potential, the inhibition of reactive oxygen species production, and the decrease of mitochondrial release of cytochrome c in PC12 cells. In addition, decursin significantly suppressed the activity of caspase-3 and moderated the ratio of Bcl-2/Bax induced by Aβ25-35. These findings indicate that decursin exerts a neuroprotective effect against Aβ25-35-induced neurotoxicity in PC12 cells, at least in part, via suppressing the mitochondrial pathway of cellular apoptosis.
authors.affiliation: Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, 523-808, China, china_lovelylily@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Jikun
authors.initials: J
authors.lastname: Du
authors.forename: Liyi
authors.initials: L
authors.lastname: Zou
authors.forename: Haishan
authors.initials: H
authors.lastname: Xia
authors.forename: Tie
authors.initials: T
authors.lastname: Wu
authors.forename: Yongho
authors.initials: Y
authors.lastname: Kim
authors.forename: Yongwoo
authors.initials: Y
authors.lastname: Lee
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D029969:Angelica; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D002087:Butyrates / Q000302:isolation & purification / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D016229:Amyloid beta-Peptides; D001578:Benzopyrans; D002087:Butyrates; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; C074636:amyloid beta-protein (25-35); C101278:decursin; D020169:Caspases