title: Enzyme linking to DNA aptamers via a zinc finger as a bridge.
abstract: We propose a novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins. We achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fused firefly luciferase.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan. ikebu@cc.tuat.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Abe
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murakami
authors.forename: Atsuro
authors.initials: A
authors.lastname: Tatsumi
authors.forename: Koichi
authors.initials: K
authors.lastname: Sumida
authors.forename: Aki
authors.initials: A
authors.lastname: Kezuka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Fukaya
authors.forename: Takenori
authors.initials: T
authors.lastname: Kumagai
authors.forename: Yuko
authors.initials: Y
authors.lastname: Osawa
authors.forename: Koji
authors.initials: K
authors.lastname: Sode
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ikebukuro
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D049409:Luciferases, Firefly / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D013917:Thrombin / Q000032:analysis*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D016335:Zinc Fingers
chemical_list: D052157:Aptamers, Nucleotide; D049408:Luminescent Agents; D042461:Vascular Endothelial Growth Factor A; D049409:Luciferases, Firefly; D013917:Thrombin