title: miR-362-5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3-kinase-C2β.
abstract: miR-362-5p is down-regulated in high-risk neuroblastoma and can function as a tumor suppressor. However, its role remains poorly understood. We show that miR-362-5p is down-regulated in metastatic neuroblastoma compared with primary neuroblastoma. Overexpression of miR-362-5p inhibits cell proliferation, migration and invasion of neuroblastoma cells in vitro and suppresses tumor growth of neuroblastoma in vivo. Phosphatidylinositol 3-kinase (PI3K)-C2β is a target of miR-362-5p. Knockdown of PI3K-C2β by siRNA had a similar effect to overexpression of miR-362-5p on SH-SY5Y cells. Overexpression of PI3K-C2β partially reversed tumor-suppressive effects of miR-362-5p. We suggest that miR-362-5p suppresses neuroblastoma cell growth and motility, partially by targeting PI3K-C2β.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China. Electronic address: aibeike@126.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China. Electronic address: sdylc@aliyun.com.
authors.forename: Liucheng
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China. Electronic address: 742932527@qq.com.
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China. Electronic address: xushuai523@126.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China. Electronic address: zjhzh_1954@126.com.
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Huang
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D058541:Class II Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009447:Neuroblastoma / Q000201:enzymology / Q000473:pathology*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C581582:MIRN362 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D058541:Class II Phosphatidylinositol 3-Kinases
keywords: Motility; Neuroblastoma; Phosphatidylinositol 3-kinase; Proliferation; miR-362-5p