title: Hydrophilic Nb⁵⁺-immobilized magnetic core-shell microsphere--A novel immobilized metal ion affinity chromatography material for highly selective enrichment of phosphopeptides.
abstract: Rapid and selective enrichment of phosphopeptides from complex biological samples is essential and challenging in phosphorylated proteomics. In this work, for the first time, niobium ions were directly immobilized on the surface of polydopamine-coated magnetic microspheres through a facile and effective synthetic route. The Fe3O4@polydopamine-Nb(5+) (denoted as Fe3O4@PD-Nb(5+)) microspheres possess merits of high hydrophilicity and good biological compatibility, and demonstrated low limit of detection (2 fmol). The selectivity was also basically satisfactory (β-casein:BSA=1:500) to capture phosphopeptides. They were also successfully applied for enrichment of phosphopeptides from real biological samples such as human serum and nonfat milk. Compared with Fe3O4@PD-Ti(4+) microspheres, the Fe3O4@PD-Nb(5+) microspheres exhibit superior selectivity to multi-phosphorylated peptides, and thus may be complementary to the conventional IMAC materials.
authors.affiliation: Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203, China.
authors.forename: Xueni
authors.initials: X
authors.lastname: Sun
authors.affiliation: Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203, China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: yanli@fudan.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.affiliation: Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203, China.
authors.forename: Gengli
authors.initials: G
authors.lastname: Duan
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D008280:Magnetics; D008863:Microspheres; D008892:Milk / Q000378:metabolism; D009556:Niobium / Q000737:chemistry*; D010748:Phosphopeptides / Q000032:analysis* / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D012709:Serum Albumin / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D007211:Indoles; D007477:Ions; D010748:Phosphopeptides; D011108:Polymers; D012709:Serum Albumin; C568283:polydopamine; D009556:Niobium; D014357:Trypsin; D052203:Ferrosoferric Oxide
keywords: Immobilized metal ion affinity chromatography; Magnetic microspheres; Niobium ions; Phosphopeptides