title: Assessment of biochemical and antioxidative status in patients suffering from dengue fever.
abstract: A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever (DF) by evaluating biochemical and oxidative status of patients. ELISA IgM antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) showed significant difference. Significant increases in serum alanine amino transferase (ALT) (P=0.000), aspartate amino transferase (AST) (P=0.000), alkaline phosphatase (ALP) (P=0.000), malondialdehyde (MDA) along with significant decreases in total protein (TP) (P=0.000), reduced glutathione (GSH) (P=0.000), superoxide dismutase (SOD), catalase (CAT) (P=0.000), and sialic acid contents (P=0.016) were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants (r=0.656, P=0.016). In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.
authors.affiliation: Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Rasool
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Arif
authors.initials: A
authors.lastname: Malik
authors.affiliation: Fatima Jinnah Medical College, Sir Gangaram Hospital Lahore, Lahore, 54000, Pakistan.
authors.forename: Khalid Mahmud
authors.initials: KM
authors.lastname: Khan
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Muhammad Saeed
authors.initials: MS
authors.lastname: Qureshi
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Beenish
authors.initials: B
authors.lastname: Shabbir
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Sara
authors.initials: S
authors.lastname: Zahid
authors.affiliation: Department of Biotechnology and Informatics, BUITEMS, Quetta, 87300, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asif
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Manan
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Sana
authors.initials: S
authors.lastname: Rashid
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Saima Rubab
authors.initials: SR
authors.lastname: Khan
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Hafiz Muhammad
authors.initials: HM
authors.lastname: Arsalan
authors.affiliation: Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Rabail
authors.initials: R
authors.lastname: Alam
authors.affiliation: Center for Research in Molecular Medicine, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Mahwish
authors.initials: M
authors.lastname: Arooj
authors.affiliation: Center for Research in Molecular Medicine, the University of Lahore, Lahore, 54000, Pakistan.
authors.forename: Mahmood Husain
authors.initials: MH
authors.lastname: Qazi
authors.affiliation: Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
authors.forename: Adeel Gulzar Ahmed
authors.initials: AGA
authors.lastname: Chaudhary
authors.affiliation: KACST Technology Innovation Center in Personalized Medicine, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
authors.forename: Adel Mohammed
authors.initials: AM
authors.lastname: Abuzenadah
authors.affiliation: Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
authors.forename: Mohammed Hussain
authors.initials: MH
authors.lastname: Al-Qahtani
authors.affiliation: Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. skarim1@kau.edu.sa.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Karim
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood*; D002681:China; D003715:Dengue / Q000145:classification* / Q000175:diagnosis* / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D007075:Immunoglobulin M