title: Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment.
abstract: Nitrate removal is essential for the sustainable operation of recirculating aquaculture system (RAS). This study evaluated the heterotrophic denitrification using poly(butylene succinate) as carbon source and biofilm carrier for RAS wastewater treatment. The effect of varied operational conditions (influent type, salinity and nitrate loading) on reactor performance and microbial community was investigated. The high denitrification rates of 0.53 ± 0.19 kg NO3(-)-N m(-3) d(-1) (salinity, 0‰) and 0.66 ± 0.12 kg NO3(-)-Nm(-3) d(-1) (salinity, 25‰) were achieved, and nitrite concentration was maintained below 1mg/L. In addition, the existence of salinity exhibited more stable nitrate removal efficiency, but caused adverse effects such as excessive effluent dissolved organic carbon (DOC) and dissimilation nitrate reduce to ammonia (DNRA) activity. The degradation of PBS was further confirmed by SEM and FTIR analysis. Illumina sequencing revealed the abundance and species changes of functional denitrification and degradation microflora which might be the primary cause of varied reactor performance.
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Song-Ming
authors.initials: SM
authors.lastname: Zhu
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ya-Le
authors.initials: YL
authors.lastname: Deng
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: ruanyj@zju.edu.cn.
authors.forename: Yun-Jie
authors.initials: YJ
authors.lastname: Ruan
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xi-Shan
authors.initials: XS
authors.lastname: Guo
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Shi
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jia-Zheng
authors.initials: JZ
authors.lastname: Shen
mesh_terms: D017756:Aquaculture / Q000295:instrumentation*; D018441:Biofilms / Q000254:growth & development; D019149:Bioreactors / Q000382:microbiology*; D002072:Butylene Glycols / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007220:Industrial Waste / Q000517:prevention & control; D009566:Nitrates / Q000302:isolation & purification / Q000378:metabolism*; D009584:Nitrogen; D011108:Polymers / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D002072:Butylene Glycols; D007220:Industrial Waste; D009566:Nitrates; D009584:Nitrogen; D011108:Polymers; D062065:Wastewater; D014874:Water Pollutants, Chemical; C089797:bionole
keywords: Denitrification; Poly(butylene succinate); Recirculating aquaculture system; Salinity; Wastewater treatment