title: New analytical spiral tube assembly for separation of proteins by counter-current chromatography.
abstract: A new spiral column assembly for analytical separation by counter-current chromatography is described. The column is made from a plastic spiral tube support which has 12 interwoven spiral grooves. The PTFE tubing of 1.6mm ID was first flattened by extruding through a narrow slit and inserted into the grooves to make 5 spiral layers with about 60ml capacity. The performance of the spiral column assembly was tested with separation of three stable protein samples including cytochrome C, myoglobin and lysozyme in a polymer phase system composed of polyethylene glycol 1000 and dibasic potassium phosphate each at 12.5% (w/w) in water. At 2ml/min, three protein samples were well resolved in 1h. The separation time may be further shortened by application of higher revolution speed and flow rate by improving the strength of the spiral tube support in the future.
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bldg. 10, Rm 8N230, Bethesda, MD 20892, USA; College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ma
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bldg. 10, Rm 8N230, Bethesda, MD 20892, USA. Electronic address: itoy@nhlbi.nih.gov.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods; D045304:Cytochromes c / Q000032:analysis*; D009113:Muramidase / Q000032:analysis*; D009211:Myoglobin / Q000032:analysis*; D010710:Phosphates; D011092:Polyethylene Glycols; D017680:Potassium Compounds
chemical_list: D009211:Myoglobin; D010710:Phosphates; D017680:Potassium Compounds; D011092:Polyethylene Glycols; D045304:Cytochromes c; C013216:potassium phosphate; D009113:Muramidase
keywords: Counter-current chromatography; Polymer phase system; Proteins; Spiral tube