title: HLA class II susceptibility pattern for type 1 diabetes (T1D) in an Iranian population.
abstract: This study aimed to determine the HLA-DRB1/HLA-DQB1 susceptibility and protection pattern for type 1 diabetes (T1D) in a population from Hamadan, north-west of Iran. A total of 133 patients with T1D were tested for HLA-DRB1 and HLA-DQB1 alleles using PCR-SSP compared to 100 ethnic-matched healthy controls. Alleles and haplotypes frequencies were compared between both groups. The most susceptible alleles for disease were HLA-DRB1*03:01, DRB1*04:02, DQB1*02:01 and DQB1*03:02, and protective alleles were HLA-DRB1*07:01, *11:01, *13:01, *14:01 and DRB1*15 and HLA-DQB1*06:01, *06:02 and *06:03. Haplotype analysis revealed that patients with T1D had higher frequencies of DRB1*03:01-DQB1*02:01 (OR = 4.86, P < 10(-7) ) and DRB1*04:02-DQB1*03:02 (OR = 9.93, P < 10(-7) ) and lower frequencies of DRB1*07:01-DQB1*02:01 (P = 0.0005), DRB1*11:01-DQB1*03:01 (P = 0.001), DRB1*13:01-DQB1*06:03 (P = 0.002) and DRB1*15-DQB1*06:01 (P = 0.001) haplotypes compared to healthy controls. Heterozygote combination of both susceptible haplotypes (DR3/DR4) confers the highest risk for T1D (RR = 18.80, P = 4 × 10(-5) ). Additionally, patients with homozygote diplotype, DR3/DR3 and DR4/DR4, showed a similar risk with less extent to heterozygote combination (P = 0.0004 and P = 0.01, respectively). Our findings not only confirm earlier reports from Iranians but also are in line with Caucasians and partly with Asians and some African patients with T1D. Remarkable differences were the identification of DRB1*04:01-DQB1*03:02, DRB1*07:01-DQB1*03:03 and DRB1*16-DQB1*05:02 as neutral and DRB1*13:01-DQB1*06:03 as the most protective haplotypes in this study.
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Kiani
authors.affiliation: Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hajilooi
authors.affiliation: Department of Transplantation Immunology, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm & German Red Cross Blood Donor Services Baden-Württemberg-Hessia, Ulm, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Furst
authors.affiliation: Department of Transplantation Immunology, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm & German Red Cross Blood Donor Services Baden-Württemberg-Hessia, Ulm, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Rezaei
authors.affiliation: Department of Transplantation Immunology, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm & German Red Cross Blood Donor Services Baden-Württemberg-Hessia, Ulm, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Shahryari-Hesami
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Kowsarifard
authors.affiliation: Department of Transplantation Immunology, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm & German Red Cross Blood Donor Services Baden-Württemberg-Hessia, Ulm, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Zamani
authors.affiliation: Department of Transplantation Immunology, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm & German Red Cross Blood Donor Services Baden-Württemberg-Hessia, Ulm, Germany.|Psoriasis Research Center, Department of Dermatology, Farshchian Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: G
authors.initials: G
authors.lastname: Solgi
mesh_terms: D000328:Adult; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D059811:HLA-DRB1 Chains