title: Activation of G protein-coupled estrogen receptor 1 (GPER-1) decreases fluid intake in female rats.
abstract: Estradiol (E2) decreases fluid intake in the female rat and recent studies from our lab demonstrate that the effect is at least in part mediated by membrane-associated estrogen receptors. Because multiple estrogen receptor subtypes can localize to the cell membrane, it is unclear which receptor(s) is generating the anti-dipsogenic effect of E2. The G protein-coupled estrogen receptor 1 (GPER-1) is a particularly interesting possibility because it has been shown to regulate blood pressure; many drinking-regulatory systems play overlapping roles in the control of blood pressure. Accordingly, we tested the hypothesis that activation of GPER-1 is sufficient to decrease fluid intake in female rats. In support of this hypothesis we found that treatment with the selective GPER-1 agonist G1 reduced AngII-stimulated fluid intake in OVX rats. Given the close association between food and fluid intakes in rats, and previous reports suggesting GPER-1 plays a role in energy homeostasis, we tested the hypothesis that the effect of GPER-1 on fluid intake was caused by a more direct effect on food intake. We found, however, that G1-treatment did not influence short-term or overnight food intake in OVX rats. Together these results reveal a novel effect of GPER-1 in the control of drinking behavior and provide an example of the divergence in the controls of fluid and food intakes in female rats.
authors.affiliation: Department of Psychology, University at Buffalo SUNY, Buffalo, NY 14260, United States.
authors.forename: Jessica
authors.initials: J
authors.lastname: Santollo
authors.affiliation: Department of Psychology, University at Buffalo SUNY, Buffalo, NY 14260, United States. Electronic address: danielsd@buffalo.edu.
authors.forename: Derek
authors.initials: D
authors.lastname: Daniels
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D003517:Cyclopentanes / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D004326:Drinking / Q000187:drug effects*; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006706:Homeostasis / Q000187:drug effects; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D020318:Rats, Long-Evans; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000502:physiology*
chemical_list: C517943:1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone; D003517:Cyclopentanes; C520305:Gper1 protein, rat; D011804:Quinolines; D043562:Receptors, G-Protein-Coupled; D000804:Angiotensin II; D004958:Estradiol
keywords: Angiotensin II; Estradiol; Estrogen; Estrogen receptor; Food intake; GPR30