title: New rapid DNA extraction method with Chelex from Venturia inaequalis spores.
abstract: The objective of this study was to develop a rapid method to isolate DNA from Venturia inaequalis spores for use in diagnostic DNA mutation analysis. Chelex-100 resin was evaluated and compared with a well established DNA exctraction method, utilizing CTAB in order to have a robust comparison. In this research we demonstrated that Chelex-100 efficiently makes extraction of the DNA from V. inaequalis spores available for direct use in molecular analyses. Also, the quantity and quality of extracted DNA were shown to be adequate for PCR analysis. Comparatively, the quality of DNA samples isolated using Chelex method was better than those extracted using CTAB. In conclusion, the Chelex method is recommended for PCR experiments considering its simplicity and cost-effectiveness.
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale G. Fanin 46, 40127 Bologna, Italy.
authors.forename: Ceren
authors.initials: C
authors.lastname: Turan
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale G. Fanin 46, 40127 Bologna, Italy.
authors.forename: Irene Maja
authors.initials: IM
authors.lastname: Nanni
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale G. Fanin 46, 40127 Bologna, Italy.
authors.forename: Agostino
authors.initials: A
authors.lastname: Brunelli
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale G. Fanin 46, 40127 Bologna, Italy. Electronic address: marina.collina@unibo.it.
authors.forename: Marina
authors.initials: M
authors.lastname: Collina
mesh_terms: D000327:Adsorption; D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012117:Resins, Synthetic / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013172:Spores, Fungal / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D012117:Resins, Synthetic; C024997:Chelex 100
keywords: CTAB; Chelex; DNA extraction; Venturia inaequalis