title: Insights in Behavior of Variably Formulated Alginate-Based Microcapsules for Cell Transplantation.
abstract: Alginate-based microencapsulation of live cells may offer the opportunity to treat chronic and degenerative disorders. So far, a thorough assessment of physical-chemical behavior of alginate-based microbeads remains cloudy. A disputed issue is which divalent cation to choose for a high performing alginate gelling process. Having selected, in our system, high mannuronic (M) enriched alginates, we studied different gelling cations and their combinations to determine their eventual influence on physical-chemical properties of the final microcapsules preparation, in vitro and in vivo. We have shown that used of ultrapure alginate allows for high biocompatibility of the formed microcapsules, regardless of gelation agents, while use of different gelling cations is associated with corresponding variable effects on the capsules' basic architecture, as originally reported in this work. However, only the final application which the capsules are destined to will ultimately guide the selection of the ideal, specific gelling divalent cations, since in principle there are no capsules that are better than others.
authors.affiliation: Interdisciplinary Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Pia
authors.initials: P
authors.lastname: Montanucci
authors.affiliation: Interdisciplinary Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Terenzi
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Via del Liceo 1, 06164 Perugia, Italy.
authors.forename: Claudio
authors.identifier: 0000-0002-7698-8970
authors.initials: C
authors.lastname: Santi
authors.affiliation: Interdisciplinary Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Pennoni
authors.affiliation: Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Vittorio
authors.identifier: 0000-0001-8404-2918
authors.initials: V
authors.lastname: Bini
authors.affiliation: Interdisciplinary Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Teresa
authors.identifier: 0000-0002-5247-4959
authors.initials: T
authors.lastname: Pescara
authors.affiliation: Interdisciplinary Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Basta
authors.affiliation: Interdisciplinary Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, Section of Internal Medicine and Endocrine and Metabolic Sciences, University of Perugia, Via Enrico dal Pozzo, s.n.c., 06126 Perugia, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Calafiore
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000627:therapeutic use; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D002214:Capsules / Q000737:chemistry / Q000627:therapeutic use; D017690:Cell Transplantation / Q000379:methods*; D004339:Drug Compounding / Q000379:methods*; D005782:Gels / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry / Q000627:therapeutic use; D006603:Hexuronic Acids / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; C008324:mannuronic acid