title: Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy.
abstract: OBJECTIVE
  To investigate the effects of transplantation of insulin-producing cells (IPCs) in the treatment of diabetic rats after 90% pancreatectomy.
  
  
  METHODS
  Human umbilical cord mesenchymal stem cells (UCMSCs) were isolated and induced into IPCs using differentiation medium. Differentiated cells were examined by dithizone (DTZ) staining, reverse transcription-polymerase chain reaction (RT-PCR), and real-time RT-PCR. C-peptide release, both spontaneously and after glucose challenge, was measured by ELISA. IPCs were then transplanted into Sprague-Dawley rats after 90% pancreatectomy and blood glucose levels and body weight were measured.
  
  
  RESULTS
  The differentiated cells were positive for DTZ staining and expressed pancreatic β-cell related genes. C-peptide release by the differentiated cells increased after glucose challenge (380.6 ± 15.32 pmol/L vs 272.4 ± 15.32 pmol/L, P < 0.05). Further, in the cell transplantation group, blood sugar levels were significantly lower than in the sham group 2 wk after transplantation (18.7 ± 2.5 mmol/L vs 25.8 ± 1.25 mmol/L, P < 0.05). Glucose tolerance tests showed that 45 min after intraperitoneal glucose injection, blood glucose levels were significantly lower on day 56 after transplantation of IPCs (12.5 ± 4.7 mmol/L vs 42.2 ± 9.3 mmol/L, P < 0.05).
  
  
  CONCLUSIONS
  Our results show that UCMSCs can differentiate into islet-like cells in vitro under certain conditions, which can function as IPCs both in vivo and in vitro.
authors.affiliation: Ya-Bin Yu, Dian-Hua Gu, Department of Hepatobiliary Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Ya-Bin
authors.initials: YB
authors.lastname: Yu
authors.affiliation: Ya-Bin Yu, Dian-Hua Gu, Department of Hepatobiliary Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Bian
authors.affiliation: Ya-Bin Yu, Dian-Hua Gu, Department of Hepatobiliary Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Dian-Hua
authors.initials: DH
authors.lastname: Gu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002096:C-Peptide / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000209:etiology / Q000235:genetics / Q000601:surgery*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000209:etiology / Q000235:genetics / Q000601:surgery*; D005786:Gene Expression Regulation; D005951:Glucose Tolerance Test; D006801:Humans; D050417:Insulin-Secreting Cells / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D010180:Pancreatectomy; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014470:Umbilical Cord / Q000166:cytology; D059631:Wharton Jelly / Q000166:cytology
chemical_list: D001786:Blood Glucose; D002096:C-Peptide
keywords: 90% pancreatectomy; Diabetic mellitus; Human umbilical cord mesenchymal stem cells