title: Intra- and Inter-Tumoral Homogeneity of BRAF(V600E) Mutations in Melanoma Tumors.
abstract: The era of targeted therapy has introduced a new therapeutic perspective for melanoma patients. Treatment with BRAFV600 inhibitors has improved overall and disease-free survival in metastatic melanoma patients whose tumors harbor BRAFV600 mutations. Although the BRAFV600E mutation appears to have a critical role in tumor initiation, its expression during tumor progression remains controversial. In fact, various authors claim that BRAFV600E heterogeneity is evident in melanoma tumors. Herein, we investigated the pattern of BRAFV600E expression in matched primary and metastatic samples from 140 patients. Using a combination of real-time PCR and immunohistochemical analyses, we demonstrated that BRAFV600E expression is homogeneous in melanoma tumors and hypothesized that the heterogeneity described by others might be attributable to technical issues when molecular methods are used. We also demonstrated the high efficiency of the anti-BRAFV600E VE1 antibody for the detection of BRAFV600E mutations in melanoma tumors.
authors.affiliation: Department of Pathology, Medical School, Universidad Complutense, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Erica
authors.initials: E
authors.lastname: Riveiro-Falkenbach
authors.affiliation: Department of Pathology, Medical School, Universidad Complutense, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Cándida A
authors.initials: CA
authors.lastname: Villanueva
authors.affiliation: Department of Pathology, Medical School, Universidad Complutense, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: María C
authors.initials: MC
authors.lastname: Garrido
authors.affiliation: Department of Pathology, Medical School, Universidad Complutense, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Ruano
authors.affiliation: Department of Pathology, Medical School, Universidad Complutense, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: García-Martín
authors.affiliation: Department of Dermatology, Hospital Universitario de Salamanca, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Godoy
authors.affiliation: Department of Dermatology, Medical School, Instituto i+12, Universidad Complutense, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Pablo L
authors.initials: PL
authors.lastname: Ortiz-Romero
authors.affiliation: Department of Pathology, Hospital Virgen Macarena, Sevilla, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Ríos-Martín
authors.affiliation: Department of Pathology, Hospital Universitario de Salamanca, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Santos-Briz
authors.affiliation: Department of Pathology, Medical School, Universidad Complutense, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain. Electronic address: jrperalto@salud.madrid.org.
authors.forename: José L
authors.initials: JL
authors.lastname: Rodríguez-Peralto
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf