title: Targeted sequencing of cancer-associated genes in hepatocellular carcinoma using next generation sequencing.
abstract: Liver cancer is one of the most common causes of cancer-associated mortality. Hepatocellular carcinoma (HCC) is the major histological subtype among types of primary liver cancer. China is an area of high incidence of HCC, and >50% of the cases of HCC worldwide are in China. At present, the mechanism underlying the development of HCC remains to be fully elucidated, and previous studies have predominantly focused on HCC in southern and eastern China, with molecular data of the HCC cases in Western China remains limited. In the present study, a panel of 372 cancer‑associated genes were screened using a next generation sequencing platform, which included a total of 12 cases from western China. The results confirmed mutations in previously identified HCC drivers, including p53 and Kras. Additionally, mutations in several cancer genes, which had not been previously associated with HCC, were identified, including RUNX1 and JAK3. The present study provided a mutation spectrum of HCC tissue in cases from western China, assisting in the investigation of the mechanism of liver carcinogenesis.
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Jikai
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Dong
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zang
authors.affiliation: Department of Medicine, Shanghai Zhangjiang Translational Medicine Research Center, Shanghai 201203, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Medicine, Shanghai Zhangjiang Translational Medicine Research Center, Shanghai 201203, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: Department of Medicine, Shanghai Zhangjiang Translational Medicine Research Center, Shanghai 201203, P.R. China.
authors.forename: Jiangman
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Du
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D004252:DNA Mutational Analysis; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; C493728:RUNX1 protein, human; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53