title: Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin.
abstract: Alpha-syntrophin (SNTA) is an adaptor protein that regulates several signaling pathways. To analyze expression of SNTA immunoblot assays must be performed. Here, the specificity of four commercially available SNTA antibodies has been evaluated in immunoblot experiments using liver tissues of wild-type and SNTA-deficient mice. While one of the antibodies reacts with SNTA, two antibodies specifically recognize beta 2 syntrophin (SNTB2). The antigen detected by the fourth antibody has not been identified but is different from SNTA and SNTB2. Therefore, only one of the four tested antibodies is appropriate to analyze SNTA protein levels by immunoblot.
authors.affiliation: Department of Internal Medicine I, University Hospital of Regensburg, 93042 Regensburg, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Eisinger
authors.affiliation: Department of Physiology and Biophysics, University of Washington, WA, USA.
authors.forename: Stanley C
authors.initials: SC
authors.lastname: Froehner
authors.affiliation: Department of Physiology and Biophysics, University of Washington, WA, USA.
authors.forename: Marvin E
authors.initials: ME
authors.lastname: Adams
authors.affiliation: Department of Internal Medicine I, University Hospital of Regensburg, 93042 Regensburg, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Krautbauer
authors.affiliation: Department of Internal Medicine I, University Hospital of Regensburg, 93042 Regensburg, Germany. Electronic address: christa.buechler@klinik.uni-regensburg.de.
authors.forename: Christa
authors.initials: C
authors.lastname: Buechler
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000276:immunology* / Q000378:metabolism; D015151:Immunoblotting; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000172:deficiency / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D009124:Muscle Proteins / Q000172:deficiency / Q000276:immunology* / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C091825:syntrophin alpha1
keywords: Immunoblot; Knockout mice; Liver