title: Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Produces N-Acyl-Homoserine Lactone Autoinducers.
abstract: Nitrobacter winogradskyi is a chemolithotrophic bacterium that plays a role in the nitrogen cycle by oxidizing nitrite to nitrate. Here, we demonstrate a functional N-acyl-homoserine lactone (acyl-HSL) synthase in this bacterium. The N. winogradskyi genome contains genes encoding a putative acyl-HSL autoinducer synthase (nwi0626, nwiI) and a putative acyl-HSL autoinducer receptor (nwi0627, nwiR) with amino acid sequences 38 to 78% identical to those in Rhodopseudomonas palustris and other Rhizobiales. Expression of nwiI and nwiR correlated with acyl-HSL production during culture. N. winogradskyi produces two distinct acyl-HSLs, N-decanoyl-l-homoserine lactone (C10-HSL) and a monounsaturated acyl-HSL (C10:1-HSL), in a cell-density- and growth phase-dependent manner, during batch and chemostat culture. The acyl-HSLs were detected by bioassay and identified by ultraperformance liquid chromatography with information-dependent acquisition mass spectrometry (UPLC-IDA-MS). The C=C bond in C10:1-HSL was confirmed by conversion into bromohydrin and detection by UPLC-IDA-MS.
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, USA mellbyeb@science.oregonstate.edu sayavedl@science.oregonstate.edu.
authors.forename: Brett L
authors.initials: BL
authors.lastname: Mellbye
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis, Oregon, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Bottomley
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, USA mellbyeb@science.oregonstate.edu sayavedl@science.oregonstate.edu.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Sayavedra-Soto
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D009573:Nitrites / Q000378:metabolism*; D009576:Nitrobacter / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C085427:LuxI protein, Bacteria; D009573:Nitrites; D014157:Transcription Factors; C088386:homoserine lactone; D015107:4-Butyrolactone