title: In vitro activity of commercial probiotic Lactobacillus strains against uropathogenic Escherichia coli.
abstract: Urinary tract infection (UTI) is one of the most prevalent infections in humans. In ≥80% of cases, the etiologic agents are strains of uropathogenic Escherichia coli (UPEC), which commonly reside in the gastrointestinal tract. Lactobacilli have been shown to prevent UTI reoccurrence by restoring the urogenital microbiota when administered vaginally or orally. The goal of this study was to determine if commercial probiotic Lactobacillus spp. reduce or clear UPEC in vitro. Results show that it is likely that lactobacilli may, in addition to restoring a healthy urogenital microbiota through acidification of their environment, also displace adhering UPEC and cause a reduction of infection.
authors.affiliation: Nestec Ltd, Nestlé Research Center, PO Box 44, CH-1000 Lausanne 26, Switzerland.
authors.forename: Michèle
authors.initials: M
authors.lastname: Delley
authors.affiliation: Nestec Ltd, Nestlé Research Center, PO Box 44, CH-1000 Lausanne 26, Switzerland.
authors.forename: Anne
authors.initials: A
authors.lastname: Bruttin
authors.affiliation: Nestec Ltd, Nestlé Research Center, PO Box 44, CH-1000 Lausanne 26, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Richard
authors.affiliation: Nestec Ltd, Nestlé Research Center, PO Box 44, CH-1000 Lausanne 26, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Affolter
authors.affiliation: Nestec Ltd, Nestlé Research Center, PO Box 44, CH-1000 Lausanne 26, Switzerland.
authors.forename: Enea
authors.initials: E
authors.lastname: Rezzonico
authors.affiliation: Nestec Ltd, Nestlé Research Center, PO Box 44, CH-1000 Lausanne 26, Switzerland Institute of Life Technologies, University of Applied Sciences Western Switzerland, CH-1950 Sion, Switzerland wolfram.bruck@hevs.ch.
authors.forename: Wolfram M
authors.initials: WM
authors.lastname: Brück
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification / Q000502:physiology*; D064307:Microbiota / Q000502:physiology; D019936:Probiotics; D019802:Succinic Acid / Q000378:metabolism; D014552:Urinary Tract Infections / Q000517:prevention & control; D056689:Uropathogenic Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D019344:Lactic Acid; D019802:Succinic Acid
keywords: Lactobacillus spp.; UPEC; UTI; bacterial coculture; cell culture; cell-free culture supernatants