title: Gene expressions of TRP channels in glioblastoma multiforme and relation with survival.
abstract: Glioblastoma multiforme (GBM) is one of the most lethal forms of cancer in humans, with a median survival of 10 to 12 months. Glioblastoma is highly malignant since the cells are supported by a great number of blood vessels. Although new treatments have been developed by increasing knowledge of molecular nature of the disease, surgical operation remains the standard of care. The TRP (transient receptor potential) superfamily consists of cation-selective channels that have roles in sensory physiology such as thermo- and osmosensation and in several complex diseases such as cancer, cardiovascular, and neuronal diseases. The aim of this study was to investigate the expression levels of TRP channel genes in patients with glioblastoma multiforme and to evaluate the relationship between TRP gene expressions and survival of the patients. Thirty-three patients diagnosed with glioblastoma were enrolled to the study. The expression levels of 21 TRP genes were quantified by using qRT-PCR with dynamic array 48 × 48 chip (BioMark HD System, Fluidigm, South San Francisco, CA, USA). TRPC1, TRPC6, TRPM2, TRPM3, TRPM7, TRPM8, TRPV1, and TRPV2 were found significantly higher in glioblastoma patients. Moreover, there was a significant relationship between the overexpression of TRP genes and the survival of the patients. These results demonstrate for the first time that TRP channels contribute to the progression and survival of the glioblastoma patients.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Alptekin
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Eroglu
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Tutar
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Sencan
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: M A
authors.initials: MA
authors.lastname: Geyik
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ulasli
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey.
authors.forename: A T
authors.initials: AT
authors.lastname: Demiryurek
authors.affiliation: Department of Medical Oncology, Faculty of Medicine, University of Gaziantep, Gaziantep, 27310, Turkey. camci@gantep.edu.tr.
authors.forename: C
authors.initials: C
authors.lastname: Camci
mesh_terms: D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D016019:Survival Analysis; D050051:Transient Receptor Potential Channels / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D050051:Transient Receptor Potential Channels
keywords: Glioblastoma multiforme; Survival; TRP channels