title: Phenotypic and Molecular Characterization of Leptospira interrogans Isolated from Canis familiaris in Southern Brazil.
abstract: Leptospirosis is a zoonotic disease caused by pathogenic spirochetes from the genus Leptospira, which includes 20 species and more than 300 serovars. Canines are important hosts of pathogenic leptospires and can transmit the pathogen to humans via infected urine. Here, we report the phenotypic and molecular characterization of Leptospira interrogans isolated from Canis familiaris in Southern Brazil. The isolated strain was characterized by variable-number tandem-repeats analysis as L. interrogans, serogroup Icterohaemorrhagiae. In addition, the isolate was recognized by antibodies from human and canine serum samples previously tested by microscopic agglutination test. Ultimately, the expression of membrane-associated antigens (LipL32 and leptospiral immunoglobulin-like proteins) from pathogenic leptospires using monoclonal antibodies was detected by indirect immunofluorescence assay. In conclusion, identification of new strains of Leptospira can help in the diagnosis and control of leptospirosis.
authors.affiliation: Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Laboratório de Vacinologia, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, CP 354, Pelotas, RS, CEP 96010-900, Brazil, sergiojorgevet@hotmail.com.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Jorge
authors.forename: Leonardo G
authors.initials: LG
authors.lastname: Monte
authors.forename: Natasha R
authors.initials: NR
authors.lastname: De Oliveira
authors.forename: Thais F
authors.initials: TF
authors.lastname: Collares
authors.forename: Bárbara C
authors.initials: BC
authors.lastname: Roloff
authors.forename: Charles K
authors.initials: CK
authors.lastname: Gomes
authors.forename: Daiane D
authors.initials: DD
authors.lastname: Hartwig
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: Cláudia P
authors.initials: CP
authors.lastname: Hartleben
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001938:Brazil; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D007921:Leptospira interrogans / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D008074:Lipoproteins / Q000032:analysis*; D018598:Minisatellite Repeats; D058889:Molecular Typing
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins