title: Efficient biosynthesis of γ-decalactone in ionic liquids by immobilized whole cells of Yarrowia lipolytica G3-3.21 on attapulgite.
abstract: In this study, the biosynthesis of γ-decalactone (GDL) was successfully conducted in an ionic liquid (IL)-containing cosolvent system using immobilized cells of Yarrowia lipolytica G3-3.21 on attapulgite (ATG). We found the immobilized Y. lipolytica G3-3.21 cells in N-butyl-pyridinium tetrafluoroborate ([BPy]BF4) solution gave the highest activity of C16-Acyl-CoA oxidase and the maximum yield of GDL. The optimum immobilization conditions for the highest yield of GDL were 20 g/L of ATG, 1.5 % of CaCl2 and 2 % of sodium alginate (NaAlg). The optimal [BPy]BF4 content, buffer pH, reaction temperature, shaking speed, castor oil and glucose contents were 7.5 %, 26 °C, 150 rpm, 100 g/L and 10 %, respectively. Under the optimized conditions, the GDL yield was up to 8.05 g/L. After ten times of reuse, the GDL yield was 7.51 g/L, corresponding to 93.3 % of that obtained in the first batch, suggesting a good reusability and potential for industrial applications.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, 223003, Jiangsu, People's Republic of China. zhaoyuping4@126.com.|Laboratory of Brewing Microbiology and Applied Enzymology, School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214036, Jiangsu, People's Republic of China. zhaoyuping4@126.com.|Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, 223003, Jiangsu, People's Republic of China. zhaoyuping4@126.com.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory of Brewing Microbiology and Applied Enzymology, School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214036, Jiangsu, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, 223003, Jiangsu, People's Republic of China.|Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, 223003, Jiangsu, People's Republic of China.
authors.forename: Changxing
authors.initials: C
authors.lastname: Jiang
mesh_terms: D018914:Cells, Immobilized; D052578:Ionic Liquids / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D017616:Magnesium Compounds / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry*; D025062:Yarrowia / Q000166:cytology / Q000378:metabolism*
chemical_list: D052578:Ionic Liquids; D007783:Lactones; D017616:Magnesium Compounds; D017655:Silicon Compounds; C109757:decan-4-olide; C026325:attapulgite
keywords: Bioconversion; Immobilized cell; Ionic liquid; Yarrowia lipolytica G3-3.21; γ-decalactone