title: Evaluation of cell-line-derived xenograft tumours as controls for immunohistochemical testing for ER and PR.
abstract: Quality control (QC) for immunohistochemistry (IHC) analysis routinely incorporates archived specimens for on-slide control material. We have assessed the utility of cell-line-derived xenograft (CDX) tumours for QC in breast estrogen receptor (ER) and progesterone receptor (PR) biomarker testing. Immunoblot and IHC analyses were used to select cell lines with different steady-state levels of ER and PR expression. CDX tumours all demonstrated consistent and comparable expression of ER and PR with corresponding cell lines from which they were derived. Three pathologists experienced in breast biomarker reporting scored tumours from different locations on mammary fat pads to determine reproducibility. Tumours from different locations were consistently scored as identical, and the CDX tumours representing different levels of biomarker expression were similar to patient-derived controls. Pathologists could not consistently distinguish CDX tumours from patient-derived controls, suggesting that within the appropriate quality management setting, CDX tumours may serve as control material for reporting purposes.
authors.affiliation: Division of Biomedical Sciences, Faculty of Medicine, Memorial University, St John's, Newfoundland, Canada.
authors.forename: Tahrim
authors.initials: T
authors.lastname: Hasan
authors.affiliation: Division of Anatomical Pathology, Laboratory Medicine Program, Eastern Health, St. John's, Newfoundland, Canada.
authors.forename: Beverley
authors.initials: B
authors.lastname: Carter
authors.affiliation: Division of Anatomical Pathology, Laboratory Medicine Program, Eastern Health, St. John's, Newfoundland, Canada.
authors.forename: Nash
authors.initials: N
authors.lastname: Denic
authors.affiliation: Division of Anatomical Pathology, Laboratory Medicine Program, Eastern Health, St. John's, Newfoundland, Canada.
authors.forename: Luis
authors.initials: L
authors.lastname: Gai
authors.affiliation: Division of Anatomical Pathology, Laboratory Medicine Program, Eastern Health, St. John's, Newfoundland, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Power
authors.affiliation: Division of Anatomical Pathology, Laboratory Medicine Program, Eastern Health, St. John's, Newfoundland, Canada.
authors.forename: Kim
authors.initials: K
authors.lastname: Voisey
authors.affiliation: Division of Biomedical Sciences, Faculty of Medicine, Memorial University, St John's, Newfoundland, Canada Division of Anatomical Pathology, Laboratory Medicine Program, Eastern Health, St. John's, Newfoundland, Canada.
authors.forename: K R
authors.initials: KR
authors.lastname: Kao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D011786:Quality Control; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D015203:Reproducibility of Results; D046888:Tissue Array Analysis
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
keywords: BREAST CANCER; IMMUNOCYTOCHEMISTRY; QUALITY CONTROL