title: Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads.
abstract: FLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays.
authors.affiliation: Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.
authors.forename: Erica
authors.initials: E
authors.lastname: Gerace
authors.affiliation: Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA. Electronic address: danesh@hms.harvard.edu.
authors.forename: Danesh
authors.initials: D
authors.lastname: Moazed
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001671:Biochemistry / Q000379:methods; D002021:Buffers; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae; D012568:Schizosaccharomyces; D012685:Sepharose / Q000737:chemistry*; D012996:Solutions
chemical_list: D000906:Antibodies; D002021:Buffers; D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides; D011506:Proteins; D012996:Solutions; D012685:Sepharose
keywords: Anti-FLAG M2 agarose beads; Anti-FLAG tag antibody; Cell lysates; FLAG; Immunoprecipitation; Mock-Elution; Peptide elution