title: Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.
abstract: In this study, the protective effect of the total saponins from Rosa laevigata Michx (RLTS) against liver fibrosis induced by carbon tetrachloride (CCl4) in rats was evaluated. The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays. Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation. In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our data indicate that RLTS can be applied as one effective candidate for the treatment of liver fibrosis in the future.
authors.affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. deshidong@163.com.|The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China. deshidong@163.com.
authors.forename: Deshi
authors.initials: D
authors.lastname: Dong
authors.affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. yinlianhong1015@163.com.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. friendqy@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. xulina627@163.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. yxy@dlmedu.edu.cn.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Peng
mesh_terms: D000199:Actins / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity; D003094:Collagen / Q000378:metabolism; D015536:Down-Regulation; D005353:Fibronectins / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000199:Actins; D000410:Alanine Transaminase; D001219:Aspartate Aminotransferases; D002251:Carbon Tetrachloride; D003094:Collagen; D005353:Fibronectins; D005978:Glutathione; D006909:Hydroxyproline; D008315:Malondialdehyde; D020782:Matrix Metalloproteinases; D012503:Saponins; D013482:Superoxide Dismutase; C498862:Acta2 protein, rat
keywords: Rosa laevigata Michx; liver fibrosis; protective effect; total saponins