title: Promoting Cell Survival and Proliferation in Degradable Poly(vinyl alcohol)-Tyramine Hydrogels.
abstract: A photopolymerizable-tyraminated poly(vinyl alcohol) (PVA-Tyr) system that has the ability to covalently bind proteins in their native state was evaluated as a platform for cell encapsulation. However, a key hurdle to this system is the radicals generated during the cross-linking that can cause oxidative stress to the cells. This research hypothesized that incorporation of anti-oxidative proteins (sericin and gelatin) into PVA-Tyr gels would mitigate any toxicity caused by the radicals. The results showed that although incorporation of 1 wt% sericin promoted survival of the fibroblasts, both sericin and gelatin acted synergistically to facilitate long-term 3D cell function. The encapsulated cells formed clusters with deposition of laminin and collagen, as well as remaining metabolically active after 21 d.
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052.
authors.forename: Khoon S
authors.initials: KS
authors.lastname: Lim
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052.
authors.forename: Yogambha
authors.initials: Y
authors.lastname: Ramaswamy
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052.
authors.forename: Justine J
authors.initials: JJ
authors.lastname: Roberts
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052.
authors.forename: Marie-Helene
authors.initials: MH
authors.lastname: Alves
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Poole-Warren
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052. p.martens@unsw.edu.au.
authors.forename: Penny J
authors.initials: PJ
authors.lastname: Martens
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry; D005780:Gelatin / Q000494:pharmacology; D020100:Hydrogels / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D047030:Sericins / Q000494:pharmacology; D034421:Sus scrofa; D014439:Tyramine / Q000494:pharmacology*
chemical_list: D005609:Free Radicals; D020100:Hydrogels; D047030:Sericins; D000255:Adenosine Triphosphate; D005780:Gelatin; D011142:Polyvinyl Alcohol; D014439:Tyramine
keywords: antioxidant; biomaterials; cell encapsulation; hydrogels; photopolymerization