title: Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses.
abstract: Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess, and sulfur deficiency. A home-built RT-qPCR mirEX platform for the amplification of 289 Arabidopsis microRNA transcripts was used to study their response to abiotic stresses. Small RNA sequencing, Northern hybridization, and TaqMan® microRNA assays were performed to study the abundance of mature microRNAs. A broad response on the level of primary miRNAs (pri-miRNAs) was observed. However, stress response at the level of mature microRNAs was rather confined. The data presented show that in most instances, the level of a particular mature miRNA could not be predicted based on the level of its pri-miRNA. This points to an essential role of posttranscriptional regulation of microRNA expression. New Arabidopsis microRNAs responsive to abiotic stresses were discovered. Four microRNAs: miR319a/b, miR319b.2, and miR400 have been found to be responsive to several abiotic stresses and thus can be regarded as general stress-responsive microRNA species.
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Barciszewska-Pacak
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Kaja
authors.initials: K
authors.lastname: Milanowska
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Knop
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Bielewicz
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Nuc
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Plewka
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Andrzej M
authors.initials: AM
authors.lastname: Pacak
authors.affiliation: Zurich-Basel Plant Science Center, Swiss Plant Science Web, Botanical Institute, University of Basel Basel, Switzerland.
authors.forename: Franck
authors.initials: F
authors.lastname: Vazquez
authors.affiliation: Department of Computational Biology, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Karlowski
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Jarmolowski
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Szweykowska-Kulinska
keywords: abiotic stress; gene expression; miRNA; pri-miRNA