title: Lactobionic acid production by glucose-fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli.
abstract: OBJECTIVE
  To use the glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis and expressed in Escherichia coli for lactobionic acid production by conversion of lactose from whey.
  
  
  RESULTS
  The highest concentrations of lactobionic acid (3.2 mg ml(-1)) during oxidation of whey-derived lactose by E. coli was at 24 h. Introduction of GFOR gene from Z. mobilis, into E. coli improved enzyme yields compared to what is obtainable by fermentation of the donor strain. The production of lactobionic acid by E. coli was 2.6-times higher than by Z. mobilis.
  
  
  CONCLUSIONS
  Recombinants of E. coli overexpressing the GFOR gene from Z. mobilis produced higher amount of lactobionoic acid from whey-derived lactose.
authors.affiliation: Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Fermentation and Biosynthesis, Institute of Food Technology of Plant Origin, Wojska Polskiego 31, 60-624, Poznan, Poland. kamilag@up.poznan.pl.
authors.forename: Kamila
authors.initials: K
authors.lastname: Goderska
authors.affiliation: Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60-627, Poznan, Poland. w_juzwa@up.poznan.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Juzwa
authors.affiliation: Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Fermentation and Biosynthesis, Institute of Food Technology of Plant Origin, Wojska Polskiego 31, 60-624, Poznan, Poland. artursz@up.poznan.pl.
authors.forename: Artur
authors.initials: A
authors.lastname: Szwengiel
authors.affiliation: Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Fermentation and Biosynthesis, Institute of Food Technology of Plant Origin, Wojska Polskiego 31, 60-624, Poznan, Poland. zbyczar@up.poznan.pl.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Czarnecki
mesh_terms: D004187:Disaccharides / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology* / Q000235:genetics
chemical_list: D004187:Disaccharides; D011994:Recombinant Proteins; C005608:lactobionic acid; D010088:Oxidoreductases; C074291:glucose-fructose oxidoreductase
keywords: Escherichia coli; Glucose–fructose oxidoreductase; Lactobionic acid; Lactose; Whey; Zymomonas mobilis