title: Genipin-crosslinked electrospun chitosan nanofibers: Determination of crosslinking conditions and evaluation of cytocompatibility.
abstract: To improve durability in wet conditions, electrospun chitosan (CTS) nanofibers were submersed into PBS (pH 7.4) solutions containing varied amounts of genipin (GP 0.1, 0.5, and 1% w/v) for crosslinking treatment. GP-crosslinking allowed the electrospun CTS nanofibers to maintain their fibrous morphology in wet state. Maximum tensile strength, 84.2% of the dry state strength, was attained when crosslinking was performed in GP 0.5% solution. GP-crosslinking also endowed the CTS nanofibers with enhanced resistances to swelling and enzymatic degradation. GP-crosslinked CTS nanofibers were found to significantly promote the adhesion and growth of the L929 fibroblasts, with the most suitable sample was the one crosslinked in the GP 0.5% solution as well. Our results suggest that crosslinking with the 0.5% GP in PBS could yield CTS nanofibers with improved wet stability in nanofiber structure and optimized mechanical and biological performances.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Xianliu
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Xiangxin
authors.initials: X
authors.lastname: Lou
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Yuan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Tu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Biyun
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. Electronic address: yzzhang@dhu.edu.cn.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D039823:Iridoids / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D039823:Iridoids; D048271:Chitosan; C007834:genipin; D009113:Muramidase
keywords: Chitosan; Crosslinking; Cytocompatibility; Electrospinning; Genipin