title: Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides.
abstract: Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation in wheat improvement. Here, we report next-generation sequencing of the flow-sorted chromosome 5B of T. dicoccoides to shed light into its genome structure, function and organization by exploring the repetitive elements, protein-encoding genes and putative microRNA and tRNA coding sequences. Comparative analyses with its counterparts in modern and wild wheats suggest clues into the B-genome evolution. Syntenic relationships of chromosome 5B with the model grasses can facilitate further efforts for fine-mapping of traits of interest. Mapping of 5B sequences onto the root transcriptomes of two additional T. dicoccoides genotypes, with contrasting drought tolerances, revealed several thousands of single nucleotide polymorphisms, of which 584 shared polymorphisms on 228 transcripts were specific to the drought-tolerant genotype. To our knowledge, this study presents the largest genomics resource currently available for T. dicoccoides, which, we believe, will encourage the exploitation of its genetic and genomic potential for wheat improvement to meet the increasing demand to feed the world.
authors.affiliation: Sabanci University Nanotechnology Research and Application Centre (SUNUM), Sabanci University, Orhanlı, 34956 Tuzla, Istanbul, Turkey.
authors.forename: Bala Ani
authors.initials: BA
authors.lastname: Akpinar
authors.affiliation: Sabanci University Nanotechnology Research and Application Centre (SUNUM), Sabanci University, Orhanlı, 34956 Tuzla, Istanbul, Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Yuce
authors.affiliation: Sabanci University Nanotechnology Research and Application Centre (SUNUM), Sabanci University, Orhanlı, 34956 Tuzla, Istanbul, Turkey.
authors.forename: Stuart
authors.initials: S
authors.lastname: Lucas
authors.affiliation: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Slechtitelu 31, CZ-78371 Olomouc, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Vrána
authors.affiliation: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Slechtitelu 31, CZ-78371 Olomouc, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Burešová
authors.affiliation: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Slechtitelu 31, CZ-78371 Olomouc, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
authors.affiliation: 1] Sabanci University Nanotechnology Research and Application Centre (SUNUM), Sabanci University, Orhanlı, 34956 Tuzla, Istanbul, Turkey [2] Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering, Sabanci University, Orhanlı, 34956 Tuzla, Istanbul, Turkey.
authors.forename: Hikmet
authors.initials: H
authors.lastname: Budak
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D019143:Evolution, Molecular; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D012343:RNA, Transfer