title: Reversal of a Single Base-Pair Step Controls Guanine Photo-Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex.
abstract: Small changes in DNA sequence can often have major biological effects. Here the rates and yields of guanine photo-oxidation by Λ-[Ru(TAP)2(dppz)](2+) have been compared in 5'-{CCGGATCCGG}2 and 5'-{CCGGTACCGG}2 using pico/nanosecond transient visible and time-resolved IR (TRIR) spectroscopy. The inefficiency of electron transfer in the TA sequence is consistent with the 5'-TA-3' versus 5'-AT-3' binding preference predicted by X-ray crystallography. The TRIR spectra also reveal the differences in binding sites in the two oligonucleotides.
authors.affiliation: School of Chemistry, Trinity College, Dublin 2 (Ireland). keanepa@tcd.ie.|Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6AD (UK). keanepa@tcd.ie.
authors.forename: Páraic M
authors.initials: PM
authors.lastname: Keane
authors.affiliation: School of Chemistry, Trinity College, Dublin 2 (Ireland).|Trinity Biomedical Sciences Institute, Pearse St., Dublin 2 (Ireland).
authors.forename: Fergus E
authors.initials: FE
authors.lastname: Poynton
authors.affiliation: Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6AD (UK).|Diamond Light Source, Harwell Science and Innovation campus Didcot, Oxfordshire,OX11 0QX (UK).
authors.forename: James P
authors.initials: JP
authors.lastname: Hall
authors.affiliation: Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Science and Innovation campus, Didcot, Oxfordshire,OX11 0QX (UK).
authors.forename: Igor V
authors.initials: IV
authors.lastname: Sazanovich
authors.affiliation: Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Science and Innovation campus, Didcot, Oxfordshire,OX11 0QX (UK).
authors.forename: Michael
authors.initials: M
authors.lastname: Towrie
authors.affiliation: School of Chemistry, Trinity College, Dublin 2 (Ireland).|Trinity Biomedical Sciences Institute, Pearse St., Dublin 2 (Ireland).
authors.forename: Thorfinnur
authors.initials: T
authors.lastname: Gunnlaugsson
authors.affiliation: School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4 (Ireland).
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Quinn
authors.affiliation: Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6AD (UK). c.j.cardin@rdg.ac.uk.
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Cardin
authors.affiliation: School of Chemistry, Trinity College, Dublin 2 (Ireland). jmkelly@tcd.ie.
authors.forename: John M
authors.initials: JM
authors.lastname: Kelly
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology*; D008027:Light; D008958:Models, Molecular; D010084:Oxidation-Reduction / Q000187:drug effects; D010619:Phenazines / Q000737:chemistry / Q000494:pharmacology*; D012428:Ruthenium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D010619:Phenazines; D006147:Guanine; D012428:Ruthenium; D004247:DNA
keywords: DNA; electron transfer; photooxidation; ruthenium; time-resolved spectroscopy