title: Chemoenzymatic Syntheses of Sialylated Oligosaccharides Containing C5-Modified Neuraminic Acids for Dual Inhibition of Hemagglutinins and Neuraminidases.
abstract: A fast chemoenzymatic synthesis of sialylated oligosaccharides containing C5-modified neuraminic acids is reported. Analogues of GM3 and GM2 ganglioside saccharidic portions where the acetyl group of NeuNAc has been replaced by a phenylacetyl (PhAc) or a propanoyl (Prop) moiety have been efficiently prepared with metabolically engineered E. coli bacteria. GM3 analogues were either obtained by chemoselective modification of biosynthetic N-acetyl-sialyllactoside (GM3 NAc) or by direct bacterial synthesis using C5-modified neuraminic acid precursors. The latter strategy proved to be very versatile as it led to an efficient synthesis of GM2 analogues. These glycomimetics were assessed against hemagglutinins and sialidases. In particular, the GM3 NPhAc displayed a binding affinity for Maackia amurensis agglutinin (MAA) similar to that of GM3 NAc, while being resistant to hydrolysis by Vibrio cholerae (VC) neuraminidase. A preliminary study with influenza viruses also confirmed a selective inhibition of N1 neuraminidase by GM3 NPhAc, suggesting potential developments for the detection of flu viruses and for fighting them.
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.|CNRS, CERMAV, 38000 Grenoble (France).
authors.forename: Lémonia
authors.initials: L
authors.lastname: Birikaki
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.|CNRS, CERMAV, 38000 Grenoble (France).
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Pradeau
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.|CNRS, CERMAV, 38000 Grenoble (France).
authors.forename: Sylvie
authors.initials: S
authors.lastname: Armand
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.|CNRS, CERMAV, 38000 Grenoble (France).
authors.forename: Bernard
authors.initials: B
authors.lastname: Priem
authors.affiliation: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Metepec, Puebla (México).
authors.forename: Luis
authors.initials: L
authors.lastname: Márquez-Domínguez
authors.affiliation: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Metepec, Puebla (México).
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.affiliation: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Metepec, Puebla (México).
authors.forename: Gerardo
authors.initials: G
authors.lastname: Santos-López
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.|CNRS, CERMAV, 38000 Grenoble (France).
authors.forename: Eric
authors.initials: E
authors.lastname: Samain
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.|CNRS, CERMAV, 38000 Grenoble (France).
authors.forename: Hugues
authors.initials: H
authors.lastname: Driguez
authors.affiliation: Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03. Sebastien.Fort@cermav.cnrs.fr.|CNRS, CERMAV, 38000 Grenoble (France). Sebastien.Fort@cermav.cnrs.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fort
mesh_terms: D000373:Agglutinins / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006388:Hemagglutinins / Q000378:metabolism*; D006868:Hydrolysis; D029901:Maackia / Q000378:metabolism; D060847:Metabolic Engineering; D009438:Neuraminic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012794:Sialic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D014734:Vibrio cholerae / Q000201:enzymology*
chemical_list: D000373:Agglutinins; D006388:Hemagglutinins; D009438:Neuraminic Acids; D009844:Oligosaccharides; D012794:Sialic Acids; D009439:Neuraminidase
keywords: chemoenzymatic synthesis; glycomimetics; influenza; neuraminic acids; oligosaccharides