title: Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract.
abstract: Cholesterol control is fundamental for prevention of cardiovascular disorders. In this work, the hypocholesterolemic activity of an aqueous Opuntia ficus-indica extract (AOE) was tested in triton-induced mice. The inhibitory activity on pancreatic lipase enzyme was evaluated in vitro by the same extract. Furthermore, polyphenol content of the extract was evaluated. Hypercholesterolemia was induced in three groups of mice by intraperitoneal administration of Triton WR-1339. After induction of hypercholesterolemia, the groups were treated with an AOE (500 mg/kg) and saline solution and the positive control group with orlistat, respectively. Cholesterol levels were measured 24 h later in peripheral blood. The levels of blood cholesterol after administration of AOE significantly decreased compared to negative control. The inhibitory activity of AOE on pancreatic lipase enzyme was evaluated at concentrations from 60 to 1000 μg/mL. The AOE inhibited the pancreatic lipase with an IC50 = 588.5 μg/mL. The AOE had a high content of polyphenolic compounds. These results show that AOE is able to prevent hypercholesterolemia by pancreatic lipase inhibition, in part due to its polyphenolic compounds.
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Eduardo
authors.identifier: 0000-0003-4783-841X
authors.initials: E
authors.lastname: Padilla-Camberos
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Flores-Fernandez
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Fernandez-Flores
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Yanet
authors.initials: Y
authors.lastname: Gutierrez-Mercado
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Joel
authors.initials: J
authors.lastname: Carmona-de la Luz
authors.affiliation: Instituto Tecnológico Superior de Perote, Km 2.5 Carretera Perote-México Colonia Centro, 91270 Perote, VER, Mexico.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Sandoval-Salas
authors.affiliation: Instituto Tecnológico Superior de Perote, Km 2.5 Carretera Perote-México Colonia Centro, 91270 Perote, VER, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Mendez-Carreto
authors.affiliation: Lancaster Medical School, Lancaster LA1 4YG, UK.
authors.forename: Kirk
authors.identifier: 0000-0002-5962-1704
authors.initials: K
authors.lastname: Allen
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D008049:Lipase / Q000037:antagonists & inhibitors / Q000097:blood*; D051379:Mice; D029747:Opuntia / Q000737:chemistry*; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000633:toxicity; D014280:Triglycerides / Q000097:blood
chemical_list: D010936:Plant Extracts; D014280:Triglycerides; D011092:Polyethylene Glycols; D002784:Cholesterol; D008049:Lipase; C016811:tyloxapol