title: Pulp Revascularization of Immature Dog Teeth with Apical Periodontitis Using Triantibiotic Paste and Platelet-rich Plasma: A Radiographic Study.
abstract: BACKGROUND
  This study evaluates radiographically the efficacy of 4 revascularization protocols in necrotic-infected immature dog teeth with apical periodontitis (AP).
  
  
  METHODS
  Forty double-rooted immature premolar teeth from 4 female beagle dogs aged 5 months were used. Four teeth were left untouched as negative controls; the other 36 teeth were infected to develop pulp necrosis and AP following different treatment protocols. Four teeth were left untreated and assigned to the positive control group, and the last 28 teeth were randomly assigned into 4 experimental groups of 8 teeth: A1, sodium hypochlorite (NaOCl) + a blood clot; A2, NaOCl + platelet-rich plasma (PRP); B1, NaOCl + modified triantibiotic paste (mTAP) + a blood clot; and B2, NaOCl + mTAP + PRP. Teeth were monitored radiographically for 6 months regarding healing of periapical radiolucencies, thickening of the dentinal walls, and apical closure of roots.
  
  
  RESULTS
  Significant differences (P < .05) between the 4 groups were evident in the percentage of teeth showing improvement of periapical radiolucencies (62.5%), continued radiographic thickening of radicular walls (53.1%), radiographic apical closure (43.8%), and deposition of hard tissue on radicular dentin walls (53.1%). Group B2 showed maximal improvement in the 3 variables assessed (P < .05). Group A1 showed the minimum percentages in the 3 parameters assessed (P < .05).
  
  
  CONCLUSIONS
  These results suggest that an intracanal dressing of mTAP and the use of PRP as scaffold improves the success rate of the revascularization procedure.
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, Sevilla, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Rodríguez-Benítez
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, Sevilla, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Stambolsky
authors.affiliation: Virgen Macarena University Hospital, Andalusian Health Service, University of Sevilla, Sevilla, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Gutiérrez-Pérez
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, Sevilla, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Torres-Lagares
authors.affiliation: Department of Stomatology, School of Dentistry, University of Sevilla, Sevilla, Spain. Electronic address: segurajj@us.es.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Segura-Egea
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001641:Bicuspid / Q000098:blood supply / Q000000981:diagnostic imaging / Q000187:drug effects*; D017610:Calcium Compounds / Q000494:pharmacology; D003782:Dental Pulp / Q000098:blood supply*; D004195:Disease Models, Animal; D004285:Dogs; D004338:Drug Combinations; D005260:Female; D054843:Negative-Pressure Wound Therapy; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D010087:Oxides / Q000494:pharmacology; D010485:Periapical Periodontitis / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D010511:Periodontal Dressings; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D017640:Silicates / Q000494:pharmacology; D012973:Sodium Hypochlorite; D016896:Treatment Outcome
chemical_list: D017607:Aluminum Compounds; D000900:Anti-Bacterial Agents; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate; D012973:Sodium Hypochlorite
keywords: Apical negative pressure irrigation; immature tooth; mineral trioxide aggregate; platelet-rich plasma; pulp regeneration; pulp revascularization; scaffold; triantibiotic paste