title: Association of -308G/A and -238G/A polymorphisms of TNF-α and osteosarcoma risk.
abstract: OBJECTIVE
  As a proinflammatory cytokine, TNF-α is associated with increased risk of osteosarcoma (OS). Our study aimed to explore the association of TNF-α polymorphisms and OS susceptibility in the Han Chinese population.
  
  
  METHODS
  80 OS patients and 99 healthy people, matched on the age and sex, participated in the study. Genotyping was conducted by the method of polymerase chain reaction-restricted fragment length polymorphisms (PCR-RFLP). Then logistic regression was used to evaluate the effects of TNF-α polymorphisms (-308 G/A and -238 G/A) on the pathology of OS.
  
  
  RESULTS
  The frequency of AA genotype in -308 G/A locus in the cases was significantly higher than that of the healthy group (20.0% vs. 6.1%). Patients with OS were more likely to possess AA genotype of -308 G/A locus (OR=4.00, 95% CI=1.41-11.38). For the patients with A allele, the risk for OS increased 0.62 fold (OR=1.62, 95% CI=1.04-2.50). There was no remarkable relationship of -238 G/A polymorphisms and OS susceptibility. In addition, we found that patients with G-A and A-A haplotypes was much higher in the cases than that of control group (68.0% and 25.0%, 53.0% and 38.9%, respectively). A-G haplotype appeared to increase the risk for OS (OR=1.93, 95% CI=1.13-2.94).
  
  
  CONCLUSIONS
  The AA genotype of -308 G/A locus of TNF-α gene was a risk factor for OS, however there was no correlation between -238 G/A of TNF-α and OS.
authors.affiliation: Department of Orthopaedics, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100835, China ; Department of Orthopaedics, The Puren Hospital of Beijing 100 Chongwai Street, Beijing 100062, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100835, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Orthopaedics, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100835, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Spine Surgery, Beijing Bo'ai Hospital, China Rehabilitation Research Center Beijing 100068, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Chinese People's Liberation Army General Hospital 28 Fuxing Road, Beijing 100835, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics; D001859:Bone Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000208:ethnology / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D010641:Phenotype; D011110:Polymorphism, Genetic; D018570:Risk Assessment; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha
keywords: -238G/A; -308G/A; TNF-α; osteosarcoma; polymorphisms; susceptibility