title: The master switch: Comparative study of mast cell in oral epithelial dysplasia, oral submucous fibrosis and oral squamous cells carcinoma and their association with inflammation and angiogenesis.
abstract: BACKGROUND
  Dental and medical practitioners encounter wide spectrum of oral lesions in their day-to-day practice. Many of the lesions such as leukoplakia, oral submucous fibrosis (OSF), etc., are associated with tobacco and betel nut chewing. Oral leukoplakia, OSF, oral lichen planus and oral squamous cell carcinoma (OSCC) are the most commonly occurring oral diseases associated with characteristic clinical and histological features and are associated with chronic inflammation at some stage of the disease process.
  
  
  OBJECTIVE
  To study and compare the number, morphology and topographical distribution of mast cells in oral epithelial dysplasia (OED), OSF and OSCC and to correlate different types of mast cells with the inflammatory infiltrate and vascularity of the lesions.
  
  
  METHODS
  Total number of subjects was 120 and equally divided into four groups of 30 as controls, OED, OSF and OSCC cases. Two sections of from each tissue embedded in paraffin wax block were made which were stained with hematoxylin and eosin and toluidine blue stain. Mast cells were counted in five different zones.
  
  
  RESULTS
  In the present study, increased numbers of mast cells were seen in all lesions. The cases with mild, moderate and severe inflammation showed increased number of typical (TMCs), atypical (AMCs) and granular mast cells (GMCs), respectively.
  
  
  CONCLUSIONS
  The result of the present study concludes that the mast cells play a key role in mediating the cross links between external angiogenic agent and local immunologic factors.
authors.affiliation: Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davanagere, Karnataka, India.
authors.forename: Neethu
authors.initials: N
authors.lastname: Telagi
authors.affiliation: Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davanagere, Karnataka, India.
authors.forename: B R
authors.initials: BR
authors.lastname: Ahmed Mujib
authors.affiliation: Department of Oral Pathology and Microbiology, Kamineni Dental College, Akkenapalle Vari Lingotom, Telangana, India.
authors.forename: Pavan G
authors.initials: PG
authors.lastname: Kulkarni
authors.affiliation: Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davanagere, Karnataka, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Naik
keywords: Angiogenesis; inflammation; malignant lesions; mast cells; premalignant lesions