title: [Effect of Siwu decoction on function and expression of P-glycoprotein in Caco-2 cells].
abstract: To study the effect of Siwu decoction on the function and expression of P-glycoprotein (P-gp) in Caco-2 cells. The Real-time quantitative poly-merase chain reaction (Q-PCR) was used to analyze the mRNA expression of MDR1 gene in Caco-2 cells. Flow cytometer was used to study the effect of Siwu decoction on the uptake of Rhodamine 123 in Caco-2 cells, in order to evaluate the efflux function of P-gp. Western blotting method was used to detect the effect of Siwu decoction on the P-gp protein expression of Caco-2 cells. Compared with the blank control group, after Caco-2 incubation with Siwu decoction at concentrations of 3.3, 5.0, 10.0 g x L(-1) for 24, 48, 72 h, the mRNA expression of MDR1 was up-regulated, suggesting the effect of Siwu decoction in inducing the expression of MDR1. After the administration with Siwu decoction in Caco-2 cells for 48 h, the uptake of Rhodamine 123 in Caco-2 cells decreased by respectively 16.6%, 22.1% (P < 0.05) and 45.4% (P < 0.01), indicating that the long-term administration of Siwu decoction can enhance the P-gp efflux function of Caco-2 cells. After the incubation of Caco-2 cells with Siwu decoction for 48 h, the P-gp protein expression on Caco-2 cell emebranes, demonstrating the effect of Siwu decoction in inducing the protein expression of P-gp.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Zeng-chun
authors.initials: ZC
authors.lastname: Ma
authors.forename: Xian-ju
authors.initials: XJ
authors.lastname: Huang
authors.forename: Qing
authors.initials: Q
authors.lastname: You
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Tan
authors.forename: Yu-guang
authors.initials: YG
authors.lastname: Wang
authors.forename: Qian-de
authors.initials: QD
authors.lastname: Liang
authors.forename: Xiang-lin
authors.initials: XL
authors.lastname: Tang
authors.forename: Cheng-rong
authors.initials: CR
authors.lastname: Xiao
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics / Q000378:metabolism; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics* / Q000378:metabolism*; D018938:Caco-2 Cells; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004365:Drugs, Chinese Herbal