title: 'SEEDY' (Simulation of Evolutionary and Epidemiological Dynamics): An R Package to Follow Accumulation of Within-Host Mutation in Pathogens.
abstract: Genome sequencing is an increasingly common component of infectious disease outbreak investigations. However, the relationship between pathogen transmission and observed genetic data is complex, and dependent on several uncertain factors. As such, simulation of pathogen dynamics is an important tool for interpreting observed genomic data in an infectious disease outbreak setting, in order to test hypotheses and to explore the range of outcomes consistent with a given set of parameters. We introduce 'seedy', an R package for the simulation of evolutionary and epidemiological dynamics (http://cran.r-project.org/web/packages/seedy/). Our software implements stochastic models for the accumulation of mutations within hosts, as well as individual-level disease transmission. By allowing variables such as the transmission bottleneck size, within-host effective population size and population mixing rates to be specified by the user, our package offers a flexible framework to investigate evolutionary dynamics during disease outbreaks. Furthermore, our software provides theoretical pairwise genetic distance distributions to provide a likelihood of person-to-person transmission based on genomic observations, and using this framework, implements transmission route assessment for genomic data collected during an outbreak. Our open source software provides an accessible platform for users to explore pathogen evolution and outbreak dynamics via simulation, and offers tools to assess observed genomic data in this context.
authors.affiliation: Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard T. H. Chan School of Public Health, Boston, MA, United States of America.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Worby
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA, United States of America; Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, United States of America.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Read
mesh_terms: D005075:Biological Evolution; D003198:Computer Simulation; D004196:Disease Outbreaks; D018562:Disease Transmission, Infectious; D005787:Gene Frequency; D023281:Genomics / Q000379:methods*; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D008962:Models, Theoretical; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D011159:Population Surveillance / Q000379:methods*; D012984:Software