title: miR-106a promotes growth and metastasis of non-small cell lung cancer by targeting PTEN.
abstract: MicroRNAs are a class of small non-coding RNAs that play essential roles in cancer development and progression. Recent studies suggested that abnormal expression of miRNAs occurs frequently in non-small cell lung cancer (NSCLC) tissues compared to adjacent normal tissues. In this study, we investigated the expression and the biological roles of miR-106a in non-small cell lung cancer. Our results showed that miR-106a was up-regulated in NSCLC tissues and cell lines. Inhibition of miR-106a in NSCLC cells substantially inhibited cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a direct target of miR-106a, and over-expression of miR-106a suppressed PTEN by direct binding to its 3'-untranslated region (3'-UTR). Furthermore, the presence of miR-106a was inversely correlated with PTEN in NSCLC tissues. Overall, this study suggested that miR-106a inhibited the growth and metastasis of NSCLC cells by decreasing PTEN expression. These data provide novel insights with potential therapeutic applications for the treatment of NSCLC.
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Cardio-Thoracic Surgery, The first Affiliated Hospital of Soochow University Suzhou 215006, P. R. China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Liu
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Ju
authors.initials: J
authors.lastname: Mei
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Fang-Bao
authors.initials: FB
authors.lastname: Ding
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Xiao
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Feng-Qing
authors.initials: FQ
authors.lastname: Hu
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Cardio-Thoracic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092, P. R. China.
authors.forename: Ming-Song
authors.initials: MS
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase
keywords: Non-small cell lung cancer; PTEN; invasion; miR-106a; migration; proliferation