title: Adsorption of Silver Nanoparticles onto Different Surface Structures of Chitin/Chitosan and Correlations with Antimicrobial Activities.
abstract: Size-controlled spherical silver nanoparticles (Ag NPs) can be simply prepared by autoclaving mixtures of glass powder containing silver with glucose. Moreover, chitins with varying degrees of deacetylation (DDAc < 30%) and chitosan powders and sheets (DDAc > 75%) with varying surface structure properties have been evaluated as Ag NP carriers. Chitin/chitosan-Ag NP composites in powder or sheet form were prepared by mixing Ag NP suspensions with each of the chitin/chitosan-based material at pH 7.3, leading to homogenous dispersion and stable adsorption of Ag NPs onto chitin carriers with nanoscale fiber-like surface structures, and chitosan carriers with nanoscale porous surface structures. Although these chitins exhibited mild antiviral, bactericidal, and antifungal activities, chitin powders with flat/smooth film-like surface structures had limited antimicrobial activities and Ag NP adsorption. The antimicrobial activities of chitin/chitosan-Ag NP composites increased with increasing amounts of adsorbed Ag NPs, suggesting that the surface structures of chitin/chitosan carriers strongly influence adsorption of Ag NPs and antimicrobial activities. These observations indicate that chitin/chitosan-Ag NPs with nanoscale surface structures have potential as antimicrobial biomaterials and anti-infectious wound dressings.
authors.affiliation: Research Institute, National Defense Medical College, Saitama 359-1324, Japan. ishihara@ndmc.ac.jp.|Kobe VN Beef Corporation, Bao Loc City 670000, Vietnam. ishihara@ndmc.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.affiliation: Kobe VN Beef Corporation, Bao Loc City 670000, Vietnam. imvinhs@yahoo.com.vn.
authors.forename: Vinh Quang
authors.initials: VQ
authors.lastname: Nguyen
authors.affiliation: Department of Applied Chemistry and Chemical Engineering National Institute of Technology, Toyama College, Toyama 939-8630, Japan. yamori@nc-toyama.ac.jp.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Research Institute, National Defense Medical College, Saitama 359-1324, Japan. snaka@ndmc.ac.jp.
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Research Institute, National Defense Medical College, Saitama 359-1324, Japan. h2@ndmc.ac.jp.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001458:Bandages; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D002686:Chitin; D012834:Silver; D048271:Chitosan
keywords: antimicrobial activity; chitin; chitosan; silver nanoparticles; surface structure