title: Increased brain-derived neurotrophic factor (BDNF) protein concentrations in mice lacking brain serotonin.
abstract: The interplay between BDNF signaling and the serotonergic system remains incompletely understood. Using a highly sensitive enzyme-linked immunosorbent assay, we studied BDNF concentrations in hippocampus and cortex of two mouse models of altered serotonin signaling: tryptophan hydroxylase (Tph)2-deficient (Tph2 (-/-)) mice lacking brain serotonin and serotonin transporter (SERT)-deficient (SERT(-/-)) mice lacking serotonin re-uptake. Surprisingly, hippocampal BDNF was significantly elevated in Tph2 (-/-) mice, whereas no significant changes were observed in SERT(-/-) mice. Furthermore, BDNF levels were increased in the prefrontal cortex of Tph2 (-/-) but not of SERT(-/-) mice. Our results emphasize the interaction between serotonin signaling and BDNF. Complete lack of brain serotonin induces BDNF expression.
authors.affiliation: Department of Psychiatry and Psychotherapy, Campus Charité Mitte, Charité - University Medicine Berlin, Berlin, Germany.
authors.forename: Golo
authors.initials: G
authors.lastname: Kronenberg
authors.affiliation: Research Team 'Cardiovascular Hormones and Peptides', Max-Delbruck-Center for Molecular Medicine (MDC), Robert-Roessle-Str. 10, 13125, Berlin, Germany.|School of Physiology and Pharmacology, University of Bristol, Bristol, UK.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mosienko
authors.affiliation: Department of Neurology, Charité - University Medicine Berlin, Berlin, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Gertz
authors.affiliation: Research Team 'Cardiovascular Hormones and Peptides', Max-Delbruck-Center for Molecular Medicine (MDC), Robert-Roessle-Str. 10, 13125, Berlin, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Alenina
authors.affiliation: Department of Psychiatry and Psychotherapy, Campus Charité Mitte, Charité - University Medicine Berlin, Berlin, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Hellweg
authors.affiliation: Research Team 'Cardiovascular Hormones and Peptides', Max-Delbruck-Center for Molecular Medicine (MDC), Robert-Roessle-Str. 10, 13125, Berlin, Germany. friederike.klempin@mdc-berlin.de.
authors.forename: Friederike
authors.initials: F
authors.lastname: Klempin
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006624:Hippocampus / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017397:Prefrontal Cortex / Q000737:chemistry*; D012701:Serotonin / Q000172:deficiency*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000172:deficiency; D014365:Tryptophan Hydroxylase / Q000172:deficiency
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; D012701:Serotonin; C487802:Tph2 protein, mouse; D014365:Tryptophan Hydroxylase
keywords: Antidepressant; BDNF; Depression; SERT; Serotonin; TPH2