title: Role of miR-145 in human laryngeal squamous cell carcinoma.
abstract: BACKGROUND
  Laryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. Incidences of laryngeal SCC have been reported to increase recently. In this study, we aimed to explore the biological effects of miR-145 on laryngeal cancer cells.
  
  
  METHODS
  The relative miR-145 level in laryngeal SCC tumor tissues and normal samples was investigated. Then, Hep-2 cells were utilized for functional analysis of miR-145. The proliferation abilities of transfected cells were measured using MTS assay. Scratch assay and single colony migration assay were performed to observe the alterations in migration behavior of transfected cells. Caspase assay and cell cycle analysis were used to investigate the underlying reasons of proliferative inhibition in cells in which miR-145 is overexpressed. Moreover, expression of SOX2 was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis in Hep-2 cells upon miR-145 transfection and its expression was evaluated in tumor and normal tissue sample of the larynx.
  
  
  RESULTS
  The miR-145 expression in laryngeal SCC tumor samples has been shown to be downregulated. The miR-145 overexpression caused inhibition of proliferation and migration in Hep-2 cells through induction of apoptosis and cell cycle arrest. The SOX2 level was demonstrated to be overexpressed in tumor samples and its expression was significantly decreased in miR-145 overexpressed Hep-2 cells.
  
  
  CONCLUSIONS
  We have demonstrated the deregulation of miR-145 and SOX2 in laryngeal SCC. Based on these results, we propose that miR-145, as an important regulator of SOX2, carries crucial roles in laryngeal SCC tumorigenesis.
authors.affiliation: Department of Medical Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.|Molecular Biology and Genetics Department, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Karatas
authors.affiliation: Department of Medical Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.|Advanced Genomics and Bioinformatics Research Center, The Scientific and Technological Research Council of Turkey (TUBITAK), Gebze, Kocaeli, Turkey.
authors.forename: Betul
authors.initials: B
authors.lastname: Yuceturk
authors.affiliation: Department of Medical Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Ilknur
authors.initials: I
authors.lastname: Suer
authors.affiliation: Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
authors.forename: Harun
authors.initials: H
authors.lastname: Cansiz
authors.affiliation: Department of Medical Genetics, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Solak
authors.affiliation: Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas.
authors.forename: Michael
authors.initials: M
authors.lastname: Ittmann
authors.affiliation: Department of Medical Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.|Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas.|Department of Molecular Biology and Genetics, Biruni University, Topkapi, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozen
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D014162:Transfection
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: SOX2; cancer stem cell; hsa-miR-145; laryngeal squamous cell carcinoma; stem cell marker