title: Characterization of protective immune response elicited by a trimeric envelope protein from an Indian clade C HIV-1 isolate in rhesus macaques.
abstract: BACKGROUND
  Recent preclinical studies have demonstrated the use of properly folded trimeric HIV-1 envelope proteins as immunogen for eliciting protecting immune response in macaques.
  
  
  METHODS
  Trimeric gp145 protein of Indian clade C HIV-1 (93IN101) was characterized for antigenicity by evaluating its binding to sCD4, and several monoclonal antibodies to HIV-1 by bio-layer interferometry. Ten macaques were immunized four times with purified gp145 in adjuplex adjuvant, and serum antibodies were characterized for binding to gp145 and neutralization. Immunized macaques were subjected to weekly low-dose vaginal challenge with SHIV1157-ipEL-p for 8 weeks.
  
  
  RESULTS
  Env protein elicited strong antibody response in macaques. Following challenge, seven of ten immunized macaques resisted challenge, while six of eight control animals were infected.
  
  
  CONCLUSIONS
  Env proteins from a clade C Indian isolate can elicit protective immune response and therefore may be a candidate for inclusion in a multiclade-based HIV-1 vaccine.
authors.affiliation: Advanced BioScience Laboratories, Rockville, MD, USA.
authors.forename: Veena
authors.initials: V
authors.lastname: Menon
authors.affiliation: Indian Institute of Technology Madras, Chennai, India.
authors.forename: Rangasamy Sneha
authors.initials: RS
authors.lastname: Priya
authors.affiliation: Duke University, Durham, NC, USA.
authors.forename: Celia
authors.initials: C
authors.lastname: Labranche
authors.affiliation: Duke University, Durham, NC, USA.
authors.forename: David
authors.initials: D
authors.lastname: Montefiori
authors.affiliation: Indian Institute of Technology Madras, Chennai, India.
authors.forename: Sundarasamy
authors.initials: S
authors.lastname: Mahalingam
authors.affiliation: Advanced BioScience Laboratories, Rockville, MD, USA.
authors.forename: Vaniambadi S
authors.initials: VS
authors.lastname: Kalyanaraman
authors.affiliation: Advanced BioScience Laboratories, Rockville, MD, USA.
authors.forename: Ranajit
authors.initials: R
authors.lastname: Pal
mesh_terms: D000818:Animals; D015497:HIV-1 / Q000502:physiology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000276:immunology* / Q000821:virology; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014759:Viral Envelope Proteins
keywords: gp145; mucosal challenge; vaccine