title: Influence of Navisporus floccosus (Higher Basidiomycetes) n-Hexane Extract on Arachidonate Metabolism in Vitro.
abstract: In vitro anti-inflammatory activity of 4 extracts with different polarity from the basidiomycete Navisporus floccosus was evaluated by determination of the inhibition of prostaglandin E2 formation catalyzed by purified cyclooxygenase (COX)-1 and COX-2 enzymes, and of the inhibition of leukotriene (LT) B4 formation in human polymorphonuclear leukocytes. The n-hexane extract showed the highest activity in all 3 assays. Through analysis by gas chromatography coupled with mass spectrometry (GC-MS), 9 fatty acids and fatty acid esters were identified as the major constituents of this extract. As several of them also showed inhibitory activity in the COX and LTB4 formation assays, it can be assumed that the unsaturated as well as the saturated fatty acids, and maybe also the fatty acid esters, present in the extract synergistically contribute to its in vitro anti-inflammatory activity.
authors.affiliation: Centre for Natural Product Studies, Faculty of Chemistry, University of Havana; Havana, Cuba.
authors.forename: Vivian
authors.initials: V
authors.lastname: Leliebre-Lara
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz; Graz, Austria.
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Pferschy-Wenzig
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz; Graz, Austria.
authors.forename: Ute
authors.initials: U
authors.lastname: Widowitz
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz; Graz, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Ortmann
authors.affiliation: Centre for Natural Product Studies, Faculty of Chemistry, University of Havana; Havana, Cuba.
authors.forename: Clara Nogueiras
authors.initials: CN
authors.lastname: Lima
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, 8010 Graz, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Bauer
mesh_terms: D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001487:Basidiomycota / Q000737:chemistry*; D051545:Cyclooxygenase 1 / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes; D006801:Humans; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010598:Pharmacognosy
chemical_list: D000893:Anti-Inflammatory Agents; D001095:Arachidonic Acids; D005227:Fatty Acids; D006586:Hexanes; D007975:Leukotriene B4; C026385:n-hexane; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D015232:Dinoprostone