title: Hemodynamic effects of atrial natriuretic peptide in ischemia-repertusion injury that occurs after exercise.
abstract: OBJECTIVE
  Atrial natriureticpeptide (ANP) is known as a protective agent against ischemia-reperfusion injuryfor cardiomyocytes. We compared the hemodynamic effects of ANP and isatin, which is known as an ANP receptor blocker, in ischemia followed by reperfusion in exercised rat hearts with nonexercised ones.
  
  
  METHODS
  Isolated hearts were perfused in 4 exercised (E) groups after a running protocol for 5 days and 4 nonexercised (NE) groups. In the first protocol, ANP was added to the perfusion solution before ischemia in an E and NE group. In the second protocol, different doses of isatin (0.1, 10, 100 µM/L) were added to the perfusion solution before ANP in 3 E and 3 NE groups. Left ventricular developed pressure (LVDP) and maximum and minimum rates of change in left ventricular pressure (dP/dtmax and dP/dtmin) were recorded.
  
  
  RESULTS
  Higher LVDP and dP/dtmin values were observed in the E group than the NE group following addition of ANP before ischemia. Values of dP/dtmax were higher in the E group at the first minute of reperfusion period. Hemodynamic difference was not observed between groups given the same amount of isatin before ANP.
  
  
  CONCLUSIONS
  This study indicated that higher ANP concentrations before ischemia were more effective on the left ventricle contractility and relaxation functions in the hearts that were exposed to exercise.
authors.forename: Selma Arzu
authors.initials: SA
authors.lastname: Vardar
authors.forename: Orkide
authors.initials: O
authors.lastname: Palabiyik
authors.forename: Ruhan Deniz
authors.initials: RD
authors.lastname: Topuz
authors.forename: Elif Ezgi
authors.initials: EE
authors.lastname: Gürel
authors.forename: Semra
authors.initials: S
authors.lastname: Çalişkan
authors.forename: Serap
authors.initials: S
authors.lastname: Topçu Özen
authors.forename: Necdet
authors.initials: N
authors.lastname: Süt
authors.forename: Çetin Hakan
authors.initials: ÇH
authors.lastname: Karadağ
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects*; D007510:Isatin / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control; D010805:Physical Conditioning, Animal / Q000379:methods / Q000502:physiology; D020011:Protective Agents / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017725:Ventricular Pressure / Q000187:drug effects
chemical_list: D020011:Protective Agents; D007510:Isatin; D009320:Atrial Natriuretic Factor