title: Creatine supplementation decreases plasma lipid peroxidation markers and enhances anaerobic performance in rats.
abstract: OBJECTIVE
  The study was to investigate the effects of creatine (Cr) supplementation on oxidative stress markers and anaerobic performance in rats.
  
  
  METHODS
  Sixty-four rats (Wistar) were divided into two groups: C, anaerobic exercised group (n = 32) and Cr, anaerobic exercised group supplemented with creatine (n = 32). Cr supplementation consisted of the addition of 2% Cr monohydrate to the diet. After 28 days, the rats performed acute exercise (6 × 30 seconds of vertical jumps in the water with 30 seconds rest and 50% of total body weight load attached in the back). The animals were euthanized before (pre) and at 0, 2, and 6 hours (n = 8) after acute exercise.
  
  
  RESULTS
  Acute exercise induced an increase in plasma malondialdehyde (MDA) and advanced oxidation protein products (AOPP), as well as increased total lipid hydroperoxides and AOPP in gastrocnemius muscle. Cr supplementation inhibited the formation of MDA and lipid hydroperoxides in plasma. However, the antioxidant action of Cr was observed only against AOPP in gastrocnemius muscle. Cr supplementation also increased (P < 0.05) anaerobic performance compared to the C group.
  
  
  CONCLUSIONS
  Cr supplementation is able to inhibit the increase in plasma lipid peroxidation markers induced by high-intensity and short-duration exercise in rats; equivalent actions, however, were not observed fully in muscle tissue.
authors.affiliation: a Faculty of Physical Education and Sport , State University of Londrina , Rodovia Celso Garcia Cid, Pr 445 Km 380, Campus Universitário, Londrina , Paraná , Brazil.|b Faculty of Medicine of Ribeirao Preto , University of Sao Paulo , Av. Bandeirantes 3900, Ribeirao Preto , Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Deminice
authors.affiliation: b Faculty of Medicine of Ribeirao Preto , University of Sao Paulo , Av. Bandeirantes 3900, Ribeirao Preto , Brazil.
authors.forename: Alceu Afonso
authors.initials: AA
authors.lastname: Jordao
keywords: Acute exercise; Antioxidants; Oxidative stress; Rodents