title: Circulating miR-145 is associated with plasma high-sensitivity C-reactive protein in acute ischemic stroke patients.
abstract: Stroke is a major cerebrovascular disease threatening human health and life with high morbidity, disability and mortality. We aimed to find effective biomarkers for the early diagnosis on stroke. Nine previously reported stroke-associated miRNAs (miR-21, miR-23a, miR-29b, miR-124, miR-145, miR-210, miR-221, miR-223 and miR-483-5p) were measured by quantitative real time-PCR, and plasma high-sensitivity C-reactive protein (hs-CRP) and serum interleukin 6 (IL-6), the pro-inflammation markers in brain injury, were examined by enzyme-linked immunosorbent assay in 146 acute ischemic stroke patients and 96 healthy blood donors. We found that serum miR-145 was significantly increased within 24 h after stroke onset and serum miR-23a and miR-221 were decreased in patients. Moreover, serum miR-145 was strong positively correlated with plasma hs-CRP and moderate positively correlated with serum IL-6. Meanwhile, serum miR-23a and miR-221 were moderate negatively correlated with plasma hs-CRP but not serum IL-6. Importantly, the combination of hs-CRP and serum miR-145 gained a better sensitivity/spectivity for prediction of acute ischemia stroke (area under receiver operating characteristic curve from 0.794 to 0.896). Conclusively, our preliminary findings indicate that serum miR-145 upregulated in acute ischemic stroke might be a new biomarker for acute ischemia stroke evaluation.
authors.affiliation: Department of Neurology, Liaocheng People's Hospital, Medical School of Liaocheng, Taishan Medical University, Liaocheng, Shandong Province, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Neurology, Liaocheng People's Hospital, Medical School of Liaocheng, Taishan Medical University, Liaocheng, Shandong Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hao
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng, Taishan Medical University, Liaocheng, Shandong Province, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurology, Liaocheng People's Hospital, Medical School of Liaocheng, Taishan Medical University, Liaocheng, Shandong Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D006801:Humans; D007238:Infarction / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000378:metabolism; D008875:Middle Aged; D020521:Stroke / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D015850:Interleukin-6; D035683:MicroRNAs; C508600:IL6 protein, human; C541010:MIRN145 microRNA, human; C525709:MIR221, human; C552430:MIRN23a microRNA, human
keywords: acute ischemic stroke; high-sensitivity C-reactive protein (hs-CRP); miR-145; miR-221; miR-23a