title: Production and Characterization of New Anti-HER2 Monoclonal Antibodies.
abstract: Breast cancer is a major public health problem worldwide. Although in Iran cancer is the third cause of death after coronary heart disease and accidents, mortality from cancer has been on the rise during recent decades. About 15% to 20% of patients with invasive breast cancer have abnormally high levels of HER2 protein. HER2 is a specialized protein found on breast cancer cells that controls cancer growth and spread. This study describes the generation and characterization of new anti-HER2 MAbs towards HER2 protein using a chimeric peptide immunogen containing discontinuous B-cell epitope peptide (peptide 626) and promiscuous T-helper epitope (MVF). The specificity of these MAbs was confirmed in various immunoassays, including ELISA, Western blotting, and immunofluorescence. Moreover, the MTT assay results indicated that 5H5 and 5H11 MAbs could reduce the growth of SKBR3 cells by approximately 50% (p<0.05). These MAbs that can reduce cancer cell proliferation would be useful for cancer therapy. Furthermore, the synthetic peptide used in the current work was able to induce the immune system to generate antibodies, especially IgG isotype. Therefore, it could be further used as a peptide cancer vaccine that targets different epitopes or structural domains of HER2 ECD.
authors.affiliation: 1 Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences , Isfahan, Iran .
authors.forename: Manijeh
authors.initials: M
authors.lastname: Mahdavi
authors.affiliation: 2 Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan , Isfahan, Iran .
authors.forename: Mehrnaz
authors.initials: M
authors.lastname: Keyhanfar
authors.affiliation: 1 Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences , Isfahan, Iran .
authors.forename: Abbas
authors.initials: A
authors.lastname: Jafarian
authors.affiliation: 2 Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan , Isfahan, Iran .
authors.forename: Hassan
authors.initials: H
authors.lastname: Mohabatkar
authors.affiliation: 1 Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences , Isfahan, Iran .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rabbani
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000175:diagnosis / Q000276:immunology*; D049109:Cell Proliferation; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D010446:Peptide Fragments; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human