title: Characterization of CD30/CD30L(+) Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis.
abstract: The CD30/CD30L signalling system has been implicated in the pathogenesis of several autoimmune and inflammatory conditions. In rheumatoid arthritis (RA), soluble CD30 (sCD30) levels reflect the recruitment of CD30(+) T cells into the inflamed joints and correlate with a positive response to immunosuppressive therapy. The aim of our report was to clarify the role of CD30/CD30L signalling system in the pathogenesis of RA. Our analysis of the CD30L(+) T cell subsets in peripheral blood (PB) and synovial fluid (SF) of RA patients and of the related cytokine profiles suggests the involvement of CD30/CD30L signalling in polarization of T cells towards a Th17 phenotype with proinflammatory features. Moreover, in RA SF nearly 50% of Treg cells express CD30, probably as an attempt to downmodulate the ongoing inflammation. We also show here that the engagement of CD30L on neutrophils stimulated with CD30/Fc chimera may play a crucial role in RA inflammation since activated neutrophils release IL-8, thus potentially amplifying the local inflammatory damage. In conclusion, the results obtained suggest that the complex CD30/CD30L signalling pathway is implicated in the pathogenesis and progression of RA synovitis through a concerted action on several immune effector cells.
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Alessandro
authors.identifier: 0000-0002-4780-5557
authors.initials: A
authors.lastname: Barbieri
authors.affiliation: Institute G. Gaslini, 16147 Genova, Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Dolcino
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Tinazzi
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Rigo
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Argentino
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Patuzzo
authors.affiliation: University of Genova, 16132 Genova, Italy.
authors.forename: Andrea
authors.identifier: 0000-0001-8722-3968
authors.initials: A
authors.lastname: Ottria
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Ruggero
authors.initials: R
authors.lastname: Beri
authors.affiliation: Institute G. Gaslini, 16147 Genova, Italy ; University of Genova, 16132 Genova, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Puccetti
authors.affiliation: Department of Medicine, University of Verona, 37134 Verona, Italy.
authors.forename: Claudio
authors.identifier: 0000-0001-6446-2990
authors.initials: C
authors.lastname: Lunardi
mesh_terms: D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000276:immunology*; D053285:CD30 Ligand / Q000276:immunology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology; D017730:Ki-1 Antigen / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D053285:CD30 Ligand; D016209:Interleukin-8; D017730:Ki-1 Antigen; C505774:TNFSF8 protein, human