title: Treponema DNA in bovine 'non-healing' versus common sole ulcers and white line disease.
abstract: Bovine digital dermatitis (BDD)-associated 'non-healing' white line disease (nhWLD) and 'non-healing' sole ulcers (nhSU) are increasingly encountered in cattle. Using established PCR protocols, 42 nhWLD/nhSU, 25 BDD and 15 common WLD DNA isolates were screened for the presence of Treponema DNA. Obtained amplicons were identified by gel electrophoresis and sequencing. Independent from their source, Treponema DNA was isolated from all lesions, but the lesion type varied with the detected Treponema phylotypes. Whilst Treponema pedis was omnipresent, T. medium was almost exclusively identified in BDD and associated nhWLD/nhSU lesions when compared to common WLD. This observation was confirmed by specific T. medium PCR scoring positive for all BDD and nhWLD/nhSU lesions, but for only 1/15 (6.7%) common WLD lesions. It is suggested that T. medium may have an active role in the pathogenesis of nhWLD/nhSU but further work is needed to verify this concept.
authors.affiliation: Research Group Oncology, Equine Clinic, Department of Companion Animals and Horses, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Sykora
authors.affiliation: Clinic for Ruminants, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
authors.forename: Johann
authors.initials: J
authors.lastname: Kofler
authors.affiliation: Clinic for Ruminants, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
authors.forename: Johanna
authors.initials: J
authors.lastname: Glonegger-Reichert
authors.affiliation: Clinic for Ruminants, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
authors.forename: Johanna
authors.initials: J
authors.lastname: Dietrich
authors.affiliation: Research Group Oncology, Equine Clinic, Department of Companion Animals and Horses, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria.
authors.forename: Gloria
authors.initials: G
authors.lastname: Auersperg
authors.affiliation: Research Group Oncology, Equine Clinic, Department of Companion Animals and Horses, University of Veterinary Medicine, Veterinaerplatz 1, Vienna A-1210, Austria. Electronic address: sabine.brandt@vetmeduni.ac.at.
authors.forename: Sabine
authors.initials: S
authors.lastname: Brandt
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial; D003612:Dairying; D058066:Digital Dermatitis / Q000382:microbiology*; D005260:Female; D014208:Treponema / Q000145:classification / Q000235:genetics*; D014456:Ulcer / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary*; D014945:Wound Healing
chemical_list: D004269:DNA, Bacterial
keywords: Bovine digital dermatitis; Cattle; Non-healing sole ulcers; Non-healing white line disease; Treponema