title: Administration of microparticles from blood of the lipopolysaccharide-treated rats serves to induce pathologic changes of acute respiratory distress syndrome.
abstract: This study was conducted to investigate the effect of intratracheal and intravenous administration of microparticles (MPs) on developing acute respiratory distress syndrome (ARDS). The blood MPs from lipopolysaccharide-treated rats were collected and examined by transmission electron microscopy (TEM). Cellular source of the MPs was identified by fluorescent-labeled antibodies after the circulating MPs were delivered to naïve rats. Levels of myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-10 productions in bronchoalveolar lavage fluid (BALF) and plasma were determined 24 h after the rats received intratracheal and intravenous administration of the MPs. Histopathologic examination of lungs was performed by light microscope. A TEM image of MPs showed spherical particles at a variable diameter from 0.1 to 0.5 µm. Endothelial- and leukocyte-derived vesicles were abundant in the investigated samples. Treatment with MPs may lead to significant increases in MPO, TNF-α, IL-1β, and IL-10 productions in BALF and plasma of the rats (all P < 0.001). Morphological observation indicated that alveolar structures were destroyed with a large amount of neutrophil infiltration in the lungs of the MP-treated rats. Perivascular and/or intra-alveolar hemorrhage were serious and hyaline membrane formed in the alveoli. Intratracheal and intravenous approaches to delivery of the circulating MPs to naïve recipient rats may induce ARDS. This presents an inducer of the onset of ARDS and provides potential therapeutic targets for attenuating lung injury.
authors.affiliation: Department of Respiratory Medicine, South Building, Chinese PLA General Hospital, Beijing 100853, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Special Inpatient Unit, Chinese PLA General Hospital, Beijing 100853, P. R. China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Respiratory Medicine, Special Inpatient Unit, Chinese PLA General Hospital, Beijing 100853, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liang
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100039, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Respiratory Medicine, Special Inpatient Unit, Chinese PLA General Hospital, Beijing 100853, P. R. China csh301@yeah.net gyamms@126.com.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Cai
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D055252:Cell-Derived Microparticles / Q000378:metabolism* / Q000648:ultrastructure; D016753:Interleukin-10 / Q000032:analysis / Q000097:blood; D053583:Interleukin-1beta / Q000032:analysis / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000209:etiology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000097:blood
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: Microparticles; acute respiratory distress syndrome; cytokines; myeloperoxidase; pulmonary hyaline membranes