title: Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats.
abstract: The present study was undertaken to evaluate the protective effects of genistein against cardiac inflammation and oxidative stress in streptozotocin (STZ) (45 mg/kg body weight)-induced diabetic rats. genistein (300 mg/kg/day) was administered orally for 24 weeks to STZ-induced diabetic rats. The effects of genistein on blood glucose, % glycosylated hemoglobin (HbA1c), C-reactive protein, tumor necrosis factor (TNF- α), transforming growth factor (TGF-β1), and total antioxidant were studied. Ultrastructural and histopathological assessment of injury were also undertaken using transmission electron microscope. STZ-induced diabetes resulted in significant increase in the levels of blood glucose, HbA1c, C-reactive protein, TNF- α and TGF-β1, and a decline in total antioxidant reserve of the myocardium. Administration of genistein to diabetic rats resulted in a decrease in blood glucose (p < 0.001), % HbA1c (p < 0.0001), C-reactive protein (p < 0.001), and expression of TNF- α (p < 0.001) and TGF-β1 (p < 0.0001) proteins. In addition, genistein treatment results in augmentation of total antioxidant (p < 0.01) reserve of the hearts. The above findings were supported by histological as well as immunohistochemical localization of NF-κB (p65) in the heart. Genistein treatment ameliorated the ultrastructural degenerative changes in the cardiac tissues as compared to the diabetic control. The result demonstrates that genistein restored the integrity of the diabetic myocardium by virtue of its anti-inflammatory and antioxidant effects.
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, New Delhi, 110017, India. skgup@hotmail.com.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, New Delhi, 110017, India.
authors.forename: Shirish
authors.initials: S
authors.lastname: Dongare
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, New Delhi, 110017, India.
authors.forename: Rajani
authors.initials: R
authors.lastname: Mathur
authors.affiliation: Departments of Pharmacology, MGM Medical College, Navi Mumbai, India.
authors.forename: Ipseeta Ray
authors.initials: IR
authors.lastname: Mohanty
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, New Delhi, 110017, India.
authors.forename: Sushma
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Mathur
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Tapas C
authors.initials: TC
authors.lastname: Nag
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001786:Blood Glucose / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D009202:Cardiomyopathies / Q000097:blood / Q000517:prevention & control*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications* / Q000188:drug therapy*; D004334:Drug Administration Schedule; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000008:administration & dosage* / Q000494:pharmacology; D006442:Glycated Hemoglobin / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D013311:Streptozocin; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D002097:C-Reactive Protein; D019833:Genistein; D006442:Glycated Hemoglobin; D013311:Streptozocin; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; C508960:TGFB1 protein, human; C439524:TNF protein, human; C517652:hemoglobin A1c protein, human
keywords: Diabetic cardiomyopathy; Genistein; Inflammation; Oxidative stress