title: Development of molecular markers for genetic male sterility inGossypium hirsutum.
abstract: Genetic male sterility (GMS) in cotton mediated by two homozygous recessive genes,ms5ms5andms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. Sequence analysis onms5andms6loci inGossypium hirsutumwas conducted to reveal genomic variation at these two loci between GMS and wild-typeG. hirsutuminbred lines, and sequence polymorphism linked toms5on A12 andms6on D12 was revealed. A haplotype marker set that consisted of four SNPs targeting bothms5andms6gene regions was developed and validated for association with GMS in cotton. Predictability of GMS phenotype by this haplotype SNP set was over 99 %. GMS haplotype marker set can serve as a high-throughput molecular breeding tool to select GMS individuals and improve hybrid production efficiency.
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Feng
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Don
authors.initials: D
authors.lastname: Keim
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Humphrey
authors.initials: H
authors.lastname: Wanjugi
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Issa
authors.initials: I
authors.lastname: Coulibaly
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: John
authors.initials: J
authors.lastname: Schwarz
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Huesgen
authors.affiliation: Monsanto Company, 800 N. Lindbergh Blvd, St. Louis, MO 63141 USA.
authors.forename: Seungho
authors.initials: S
authors.lastname: Cho
keywords: Genetic male sterility (GMS); Haplotype; Hybrid cotton; Molecular markers; Phenotype prediction