title: Mulberry 1-Deoxynojirimycin Inhibits Adipogenesis by Repression of the ERK/PPARγ Signaling Pathway in Porcine Intramuscular Adipocytes.
abstract: Intramuscular fat (IMF), which is modulated by adipogenensis of intramuscular adipocytes, plays a key role in pork quality associated with marbling, juiceness, and flavor. However, the regulatory mechanism of 1-deoxynojirimycin (DNJ) on adipogenesis is still unknown. Here, we found that both DNJ (2.0, 3.0, 4.0, 5.0, and 6.0 μM) and rosiglitazone (RSG; 0.1, 0.2, 0.3, 0.4, and 0.5 mM) had no effect on cell viability. Moreover, 4 μM DNJ significantly inhibited adipogenesis, whereas 0.4 mM RSG increased lipogenesis of porcine intramuscular adipocytes. Interestingly, DNJ sharply inhibited phosphorylation of extracellular regulated protein kinases 1/2 (ERK1/2), but did not change phosphorylation of AKT (protein kinase B) in intramuscular adipocytes. We further found that the inhibitory adipogenesis of DNJ was attenuated by RSG via up-regulation of PPARγ. On the basis of the above findings, we suggest that DNJ inhibited adipogenesis through the ERK/PPARγ signaling pathway in porcine intramuscular adipocytes.
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Mei-lin
authors.initials: ML
authors.lastname: Ma
authors.forename: Xiao-chang
authors.initials: XC
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.forename: Tai-yong
authors.initials: TY
authors.lastname: Yu
authors.forename: Gong-she
authors.initials: GS
authors.lastname: Yang
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Pang
mesh_terms: D017485:1-Deoxynojirimycin / Q000494:pharmacology*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D019587:Dietary Supplements / Q000032:analysis; D050155:Lipogenesis / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D031625:Morus / Q000737:chemistry*; D009132:Muscles / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D013552:Swine / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D047495:PPAR gamma; D010936:Plant Extracts; D017485:1-Deoxynojirimycin
keywords: DNJ; ERK/PPARγ signaling pathway; adipogenesis; intramuscular adipocyte; pork