title: Unnatural amino acid mutagenesis-based enzyme engineering.
abstract: Traditional enzyme engineering relies on substituting one amino acid by one of the other 19 natural amino acids to change the functional properties of an enzyme. However, incorporation of unnatural amino acids (UAAs) has been harnessed to engineer efficient enzymes for biocatalysis. Residue-specific and site-specific in vivo incorporation methods are becoming the preferred approach for producing enzymes with altered or improved functions. We describe the contribution of in vivo UAA incorporation methodologies to enzyme engineering as well as the future prospects for the field, including the integration of UAAs with other new advances in enzyme engineering.
authors.affiliation: School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea.
authors.forename: Yuvaraj
authors.initials: Y
authors.lastname: Ravikumar
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Saravanan Prabhu
authors.initials: SP
authors.lastname: Nadarajan
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Yoo
authors.affiliation: School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea.
authors.forename: Chong-soon
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea. Electronic address: hyungdon@konkuk.ac.kr.
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001709:Biotechnology; D004798:Enzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D012343:RNA, Transfer
chemical_list: D000596:Amino Acids; D004798:Enzymes; D012343:RNA, Transfer