title: Reversibly Triggered Protein-Ligand Assemblies in Giant Vesicles.
abstract: External small-molecule triggers were used to reversibly control dynamic protein-ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small-molecule substrates, thereby modulating the pH-sensitive interaction between a Ni-NTA ligand on the vesicle membrane and an oligohistidine-tagged protein in the lumen. By alternating the small-molecule substrates the interaction could be reversed.
authors.affiliation: Bio-organic chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands).
authors.forename: Ruud J R W
authors.initials: RJ
authors.lastname: Peters
authors.affiliation: Bio-organic chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands).
authors.forename: Marlies
authors.initials: M
authors.lastname: Nijemeisland
authors.affiliation: Bio-organic chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands). j.vanhest@science.ru.nl.
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D058045:Artificial Cells / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008081:Liposomes / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
chemical_list: D008024:Ligands; D008081:Liposomes; D008164:Luminescent Proteins; D011506:Proteins; D000426:Alcohol Dehydrogenase
keywords: cell mimic; liposomes; protein-ligand interactions; vesicles