title: Degradation of glutathione in Aspergillus nidulans - Short communication.
abstract: Relative transcriptions of Aspergillus nidulans dug1-3 (orthologes of Saccharomyces cerevisiae DUG - deficient in utilization of glutathione - pathway genes) and ggtA encoding γ-glutamyl transpeptidase were studied under conditions inducing glutathione degradation. GgtA was induced in all cases when glutathione levels decreased, but addition of yeast extract, which moderated glutathione degradation, enhanced its induction. Although dug2 showed constitutive transcription, dug1 and dug3 were induced by carbon and nitrogen starvation and yeast extract did not caused significant changes in their relative transcription. The in silico reconstructed DUG pathway of A. nidulans is a promising candidate for cytosolic GSH degradation induced by carbon/nitrogen stress.
authors.affiliation: Department of Biotechnology and Microbiology, University of Debrecen , Debrecen , Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Spitzmüller
authors.forename: Márton
authors.initials: M
authors.lastname: Hajdú
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000235:genetics / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D005723:gamma-Glutamyltransferase; D005978:Glutathione
keywords: Aspergillus nidulans; DUG pathway; Glutathione degradation; carbon stress; γ-glutamyl transpeptidase