title: Characterization of ESBL- and AmpC-Producing Enterobacteriaceae from Diseased Companion Animals in Europe.
abstract: The study aimed to characterize beta-lactam resistance mechanisms of Enterobacteriaceae isolates recovered from diseased dogs and cats between 2008 and 2010 in a European surveillance program (ComPath I) for the antibiotic susceptibility of bacterial pathogens. A total of 608 non-duplicated Enterobacteriaceae isolates were obtained prior antibiotic treatment from diseased dogs (n=464) and cats (n=144). Among the 608 Enterobacteriaceae isolates, 22 presented a minimal inhibitory concentration against cefotaxime above EUCAST breakpoints of susceptibility. All the 22 isolates remained susceptible to carbapenems. Ten isolates were confirmed as extended-spectrum-beta-lactamase (ESBL) producers by PCR-sequencing of bla coding genes including 9 blaCTX-M (CTX-M-1, 14, 15, 32,…) and 1 blaTEM-52 and 12 were AmpC-producing isolates (10 plasmidic CMY-2 group and 2 isolates overexpressing their chromosomal AmpC). ESBLs and plasmid-mediated AmpC (pAmpC)-producing isolates were mainly recovered from dogs (n=17) suffering from urinary tract infections (n=13) and originated from eight different countries. ESBL-bearing plasmids were mostly associated with IncFII incompatibility groups while CMY-2 was predominantly associated with plasmid of the IncI1 group. ESBL/pAmpC-producing Escherichia coli belonged to phylogroup A (n=5), B2 (n=4), and D (n=5). Multilocus sequence typing analysis revealed that among three CTX-M-15-producing E. coli, two belong to sequence type (ST) 131 and one to ST405. The presence of CTX-M-15 including on IncFII plasmids in E. coli ST131-B2 has also been described in isolates of human origin. This suggests the possibility of exchanges of these isolates from humans to companion animals or vice-versa.
authors.affiliation: 1 Belgian National Reference Center for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain , Yvoir, Belgium .
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.affiliation: 1 Belgian National Reference Center for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain , Yvoir, Belgium .
authors.forename: Te-Din
authors.initials: TD
authors.lastname: Huang
authors.affiliation: 1 Belgian National Reference Center for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain , Yvoir, Belgium .
authors.forename: Warda
authors.initials: W
authors.lastname: Bouchahrouf
authors.affiliation: 1 Belgian National Reference Center for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain , Yvoir, Belgium .
authors.forename: Caroline
authors.initials: C
authors.lastname: Bauraing
authors.affiliation: 1 Belgian National Reference Center for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain , Yvoir, Belgium .
authors.forename: Catherine
authors.initials: C
authors.lastname: Berhin
authors.affiliation: 2 ComPath Study Group, CEESA , Brussels, Belgium .
authors.forename: Farid
authors.initials: F
authors.lastname: El Garch
authors.affiliation: 1 Belgian National Reference Center for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain , Yvoir, Belgium .
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002415:Cats; D002439:Cefotaxime / Q000494:pharmacology*; D004285:Dogs; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D005060:Europe / Q000453:epidemiology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D057805:Pets / Q000382:microbiology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; D002439:Cefotaxime