title: Effects of two human chorionic gonadotropin doses administered to the ovarian states during thein vitrofertilization and embryo transfer program.
abstract: The aim of the present study was to examine the effects of the human chorionic gonadotropin (hCG) dose on the pulsatility indices (PI) of the intraovarian artery on the day of follicle aspiration and the oocyte quality, intrafollicular oxidative stress and luteinization. PI was also measured on the day of hCG administration. A total of 15 patients were undergoing thein vitrofertilization and embryo transfer (IVF-ET) program. To estimate whether there was any difference between the intraovarian artery blood flow and oocyte development of the same patients treated with 5,000 or 10,000 IU hCG, the intraovarian artery blood flow was measured by transvaginal color ultrasonography pulsed wave Doppler, and the follicular fluids and the granulosa cells were collected at follicle aspiration. There were statistically significant differences between the same patients undergoing the two different hCG-dose treatments in which the first protocol included 10,000 IU and the second protocol included 5,000 IU hCG treatment. These differences were apparent in the PI of intraovarian artery blood flow on the day of follicle aspiration (P=0.0023), in the incidence of apoptosis in cumulus (ApoC) and mural (ApoM) granulosa cells (ApoC, P=0.0077; ApoM, P=0.0128), in the total oocytes retrieved (P=0.0342) and in the follicle fluid progesterone concentration (P=0.0044). There were no significant differences between the two protocols in the PI of intraovarian artery blood flow on the day of hCG administration (P=0.4326), serum steroid on the day of follicle aspiration [serum P, P>0.9999; serum estradiol (E2), P=0.8589], follicle fluid E2concentration (P=0.8939), mature oocyte rate (P=0.3743) and total mature oocytes retrieved (P=0.2026). In conclusion, the dose of hCG administration can significantly affect the intraovarian artery blood flow and the development of follicles and oocytes in an IVF-ET program.
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Qinxi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Botao
authors.initials: B
authors.lastname: DU
authors.affiliation: Department of Obstetrics and Gynecology, Harbin Red Cross Hospital, Harbin, Heilongjiang 150076, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Yamagata University, School of Medicine, Yamagata 990-9585, Japan.
authors.forename: Baoxin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Yamagata University, School of Medicine, Yamagata 990-9585, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Saito
authors.affiliation: Department of Obstetrics and Gynecology, Yamagata University, School of Medicine, Yamagata 990-9585, Japan.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Kurachi
keywords: granulosa cells; human chorionic gonadotropin; intraovarian artery blood flow; oocyte maturation; pulsatility index