title: Effects of propofol and sevoflurane on aquaporin-4 and aquaporin-9 expression in patients performed gliomas resection.
abstract: Post-operative cerebral edema is a threat for patients performed gliomas resection. Some studies have shown that general anesthesia drugs, such as, propofol had neuroprotective effect. Aquaporin-4 (AQP4) and Aquaporin-9 (AQP9) play an important role in maintaining brain water homeostasis under various conditions. The aim of this study was to compare the effect of propofol or sevoflurane on expression of AQP4 and AQP9 in patients performed gliomas resection. 30 patients performed gliomas resection were included in this study. The patients were randomly divided into two groups: propofol group and sevoflurane group. Fresh human gliomas specimens were obtained and hematoxylin eosin (HE) staining, immunohistochemical staining and Western blot analysis were used for observation of the expression of AQP4 and AQP9. The immunohistochemical staining of the sections showed that the percentage of AQP4 positive cells in the propofol group (14.3±4.61%) was significantly lower than that in sevoflurane group (37.3±10.01%) (n=15, P<0.05). There was no significant difference in the percentage of AQP9 positive cells in propofol group and sevoflurane group (25.8±2.67 versus 28.1±7.81%, n=15, P>0.05). Western blot analysis confirmed the immunohistochemistry results. AQP4 protein level in propofol group was significantly lower than that in sevoflurane group (1.4±0.13 versus 1.7±0.12, P<0.05). Western blot analysis did not show any difference of expression of AQP9 protein between the propofol group and sevoflurane group (2.0±0.13 versus 2.1±0.13, P>0.05, n=6). AQP4 expression was lower in patients of propofol group than that in sevoflurane group. Our results suggested that propofol could inhibit the expression of AQP4.
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Wan-Chao
authors.initials: WC
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Zi-Yong
authors.initials: ZY
authors.lastname: Yue
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China. Electronic address: scy-zw@163.com.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Lu
authors.affiliation: Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affiliated Hospital of Harbin Medical University, NO 246, Xue-fu Street, Nangang District, Harbin 150081, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Chang
mesh_terms: D051401:Aquaporin 4 / Q000378:metabolism; D020346:Aquaporins / Q000378:metabolism; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D001929:Brain Edema / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D005260:Female; D005910:Glioma / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008738:Methyl Ethers / Q000627:therapeutic use*; D008875:Middle Aged; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D015742:Propofol / Q000627:therapeutic use*; D011897:Random Allocation; D000077149:Sevoflurane
chemical_list: C496030:AQP4 protein, human; C109669:AQP9 protein, human; D051401:Aquaporin 4; D020346:Aquaporins; D008738:Methyl Ethers; D018696:Neuroprotective Agents; D000077149:Sevoflurane; D015742:Propofol
keywords: AQPs; Brain edema; Glioma resection; Neuroprotection; Propofol