title: A V₂O₃-ordered mesoporous carbon composite with novel peroxidase-like activity towards the glucose colorimetric assay.
abstract: It is of great scientific and practical significance to explore inorganic mimetic enzymes to replace natural enzymes due to their instability and high cost. Herein we present an interesting discovery that a V2O3-ordered mesoporous carbon composite (V2O3-OMC) has a novel peroxidase-like activity towards fast redox reaction of typical peroxidase substrates H2O2 and 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS). Due to the small size effect and large surface area of V2O3 nanoparticles supported by OMC, V2O3-OMC exhibited excellent catalytic performance with a k(cat) of 1.28 × 10(4) s(-1), K(M) (ABTS) of 0.067 mM and K(M) (H2O2) of 0.16 mM, and a significantly higher catalytic efficiency (k(cat)/K(M)) towards the oxidation of ABTS in comparison with the natural peroxidases. Furthermore, the Ping-pong BiBi mechanism was proposed to explain the catalytic reaction by V2O3-OMC. Based on this highly active biomimetic peroxidase and the colorimetric detection of H2O2, a facile analytical method was developed to detect glucose by using V2O3-OMC and glucose oxidase, which had a wide linear range (0.01-4 mM glucose), good selectivity and reliability for successful detection of various real samples. Thus, the novel V2O3-OMC peroxidase mimetic holds great promise for broad potential applications.
authors.affiliation: Laboratory for Biosensing, Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China. liuah@qibebt.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Lingxing
authors.initials: L
authors.lastname: Zeng
authors.forename: Mingdeng
authors.initials: M
authors.lastname: Wei
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001628:Beverages; D032701:Biomimetics / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods; D002138:Calibration; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D005947:Glucose / Q000032:analysis / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*; D016062:Porosity; D015203:Reproducibility of Results; D013451:Sulfonic Acids / Q000737:chemistry; D013997:Time Factors; D014639:Vanadium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D052160:Benzothiazoles; D002241:Carbohydrates; D013451:Sulfonic Acids; D014639:Vanadium; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002244:Carbon; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D010544:Peroxidases; D005947:Glucose; D010100:Oxygen