title: The stability of water- and fat-soluble vitamin in dentifrices according to pH level and storage type.
abstract: The purpose of this study is to evaluate the vitamin stabilities in dentifrices by analyzing various vitamins according to the level and storage temperature. The stabilities of water- and fat-soluble vitamins were investigated in buffer solution at different pH values (4, 7, 8, 10 and 11) for 14 days and in dentifrices at different pH (7 and 10) for 5 months at two temperature conditions (room and refrigeration temperature) by analyzing the remaining amounts using HPLC methods. In the buffer solution, the stability of vitamins B1 , B6 and C was increased as the pH values increased. Vitamins E and K showed poor stability at pH 4, and vitamin B3 showed poor stability at pH 11. In dentifrices, the storage temperature highly influenced vitamin stability, especially vitamins C and E, but the stabilities of vitamins B1 and C according to pH values did not correspond to the buffer solution tests. Vitamin B group was relatively stable in dentifrices, but vitamin C completely disappeared after 5 months. Vitamin K showed the least initial preservation rates. Vitamins were not detected in commercial dentifrices for adults and detected amounts were less than the advertised contents in dentifrices for children.
authors.affiliation: Department of Preventive and Social Dentistry and Institue of Oral Biology, School of Dentistry, Kyung Hee University, Dongdaemoon-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: Department of Preventive and Social Dentistry and Institue of Oral Biology, School of Dentistry, Kyung Hee University, Dongdaemoon-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Ki-Eun
authors.initials: KE
authors.lastname: Kim
authors.affiliation: Department of Preventive and Social Dentistry and Institue of Oral Biology, School of Dentistry, Kyung Hee University, Dongdaemoon-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Preventive and Social Dentistry and Institue of Oral Biology, School of Dentistry, Kyung Hee University, Dongdaemoon-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Yong-Duk
authors.initials: YD
authors.lastname: Park
authors.affiliation: Department of Preventive and Social Dentistry and Institue of Oral Biology, School of Dentistry, Kyung Hee University, Dongdaemoon-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Ha-Jeong
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003802:Dentifrices / Q000737:chemistry*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D012995:Solubility; D013696:Temperature; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D003802:Dentifrices; D014815:Vitamins
keywords: HPLC; dentifrices; pH; temperature; vitamin