title: Macaúba (Acrocomia aculeata) cake from biodiesel processing: a low-cost substrate to produce lipases from Moniliella spathulata R25L270 with potential application in the oleochemical industry.
abstract: BACKGROUND
  Biodiesel industry wastes were evaluated as supplements for lipase production by Moniliella spathulata R25L270, which is newly identified yeast with great lipolytic potential. Macaúba cake (MC), used for the first time in this work as inducer to produce lipases, and residual oil (RO) were mixed to maximise enzyme production. The lipase secreted was biochemically characterised.
  
  
  RESULTS
  The best ratio for the mixture (MC:RO) was 0.66:0.34 and the fitted values for lipase activity and total protein concentration were 0.98 U mL(-1) and 0.356 mg mL(-1), respectively. Maximum activity obtained (2.47 U mL(-1)) was achieved at 31.5°C and pH 6.7, and the enzyme was stable in this condition. A novel enzyme was purified and identified for the first time by mass spectrometry. The lipase efficiently hydrolysed different natural oils and exhibited selectivity in the production of eicosapentaenoic acid from fish oil.
  
  
  CONCLUSIONS
  The use of MC and RO as a supplement to produce the new lipase from M. spathulata R25L270 may be one alternative for reducing lipase production costs and simultaneously adding value to biodiesel industry residues. The potential application of the lipase in the oleochemical industry was demonstrated by its pH and temperature stabilities and selective hydrolysis.
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Pampulha, Caixa Postal 486, Belo Horizonte, MG, 31270-901, Brazil. liviasouzabqi@gmail.com.
authors.forename: Lívia T A
authors.initials: LT
authors.lastname: Souza
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Pampulha, Caixa Postal 486, Belo Horizonte, MG, 31270-901, Brazil. jamil_lefqp@yahoo.com.br.
authors.forename: Jamil S
authors.initials: JS
authors.lastname: Oliveira
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Pampulha, Caixa Postal 486, Belo Horizonte, MG, 31270-901, Brazil. marinaqrbr@gmail.com.
authors.forename: Marina Q R B
authors.initials: MQ
authors.lastname: Rodrigues
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Caixa Postal 486, Belo Horizonte, MG, 31270-901, Brazil. verabio@gmail.com.
authors.forename: Vera L
authors.initials: VL
authors.lastname: dos Santos
authors.affiliation: Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), Campus de la Universidad Autónoma de Madrid, Nicolás Cabrera 9, 28049, Madrid, Spain. b.pessela@csic.es.
authors.forename: Benevides C
authors.initials: BC
authors.lastname: Pessela
authors.affiliation: Instituto Nanocell and Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Pampulha, Caixa Postal 486, Belo Horizonte, MG, 31270-901, Brazil. rrresende@hotmail.com.
authors.forename: Rodrigo R
authors.initials: RR
authors.lastname: Resende
mesh_terms: D028023:Arecaceae / Q000737:chemistry / Q000378:metabolism*; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D056804:Biofuels / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D007218:Industrial Microbiology / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics; D010938:Plant Oils / Q000378:metabolism; D014866:Waste Products / Q000032:analysis*
chemical_list: D056804:Biofuels; D003470:Culture Media; D005656:Fungal Proteins; D010938:Plant Oils; D014866:Waste Products; D008049:Lipase