title: Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins.
abstract: The so-called disparity mutagenesis technique selectively elevates mutation in the lagging strand of DNA by using a mutant form of DNA polymerase δ, encoded on a proofreading-deficient pol3 gene. This novel mutagenesis technique generates a pool of mutants that includes a no-mutant strain together with mutant strains carrying multiple mutations. By using a suitable screening system it is possible to isolate the desired mutant strain from this pool of mutants. Here, we used our novel mutagenesis technique to isolate a yeast strain with good growth characteristics that was glycosylation deficient.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Abe
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nakayama
mesh_terms: D004258:DNA Polymerase III / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006031:Glycosylation; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D015003:Yeasts / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins; D004258:DNA Polymerase III