title: No association between CYP17 -34T/C polymorphism and insulin resistance in Thai polycystic ovary syndrome.
abstract: OBJECTIVE
  To investigate the association of CYP 17 -34T/C polymorphism with insulin resistance (IR) in Thai polycystic ovary syndrome (PCOS).
  
  
  METHODS
  A cross-sectional study was performed on 210 Thai women diagnosed with PCOS. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze CYP17 polymorphism at -34T/C. Student's t-test was used to compare the mean of normally distributed variables between A1/A1 and A2/X. Chi-squared or Fisher's exact tests and OR were used to analyze the association at P < 0.05.
  
  
  RESULTS
  Out of 210 cases, PCR-RFLP was successful in 199. Mean patient age was 24.4 ± 4.7 years, with body mass index 25.2 ± 6.3 kg/m(2) . There were 65 and 134 women in the A1/A1 genotype group and A2/X genotype group, respectively. The A2/X genotype group was statistically significantly younger and had a strong trend toward overweight/obesity compared with the A1/A1 genotype group. The prevalence of IR according to different methods varied from 15.4% to 70.8% and was not different between the two groups. On subgroup analysis, in the overweight/obese PCOS group, the A2/X genotype was not associated with any indices of IR.
  
  
  CONCLUSIONS
  No significant association between CYP17-34T/C polymorphism and IR was found in Thai PCOS women, although the A2/X genotype group was statistically significantly younger than the A1/A1 genotype group.
authors.affiliation: Division of Gynaecologic Endocrinology Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Kitirat
authors.initials: K
authors.lastname: Techatraisak
authors.affiliation: Division of Gynaecologic Endocrinology Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Chenchit
authors.initials: C
authors.lastname: Chayachinda
authors.affiliation: Division of Gynaecologic Endocrinology Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Thanyarat
authors.initials: T
authors.lastname: Wongwananuruk
authors.affiliation: Division of Gynaecologic Endocrinology Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Chongdee
authors.initials: C
authors.lastname: Dangrat
authors.affiliation: Division of Gynaecologic Endocrinology Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Suchada
authors.initials: S
authors.lastname: Indhavivadhana
authors.affiliation: Division of Gynaecologic Endocrinology Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Manee
authors.initials: M
authors.lastname: Rattanachaiyanont
authors.affiliation: Division of Molecular Genetics, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Wanna
authors.initials: W
authors.lastname: Thongnoppakhun
mesh_terms: D000328:Adult; D000367:Age Factors; D000483:Alleles; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D050177:Overweight / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*; D013785:Thailand; D055815:Young Adult
chemical_list: D013254:Steroid 17-alpha-Hydroxylase
keywords: CYP17 promoter gene; Thai; insulin resistance; polycystic ovary syndrome; polymorphism