title: Quantification of hypoglycin A in serum using aTRAQ(®) assay.
abstract: BACKGROUND
  Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication.
  
  
  METHODS
  Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition.
  
  
  RESULTS
  Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50μmol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals.
  
  
  CONCLUSIONS
  The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
authors.affiliation: Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium. Electronic address: f.boemer@chu.ulg.ac.be.
authors.forename: François
authors.initials: F
authors.lastname: Boemer
authors.affiliation: Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium.
authors.forename: Michelle
authors.initials: M
authors.lastname: Deberg
authors.affiliation: Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium.
authors.forename: Roland
authors.initials: R
authors.lastname: Schoos
authors.affiliation: Department of animal Productions: Biostatistics, Economy and animal selection, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium.
authors.forename: Etienne
authors.initials: E
authors.lastname: Baise
authors.affiliation: Equine Pole, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium.
authors.forename: Hélène
authors.initials: H
authors.lastname: Amory
authors.affiliation: Usc 1233 Ediss, VETAGROSUP, Ecole nationale vétérinaire de Lyon, France.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Gault
authors.affiliation: LAT LUMTOX, La Voulte-sur-Rhône, France.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Carlier
authors.affiliation: LAT LUMTOX, La Voulte-sur-Rhône, France.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Gaillard
authors.affiliation: Réseau d'EpidémioSurveillance en Pathologie Equine (RESPE), Caen, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Marcillaud-Pitel
authors.affiliation: Equine Pole, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Votion
mesh_terms: D000818:Animals; D006734:Horse Diseases / Q000097:blood; D006736:Horses; D007005:Hypoglycins / Q000097:blood*; D007553:Isotope Labeling; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D009135:Muscular Diseases / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007005:Hypoglycins; C041757:hypoglycin
keywords: Amino acids; Atypical myopathy; Hypoglycin A; Mass spectrometry; aTRAQ