title: Galectin signature of the choriocarcinoma JAr cells: Galectin-1 as a modulator of invasiveness in vitro.
abstract: Our previous findings showed that galectin-1 (LGALS1) plays an important role in the in vitro invasion of normal human trophoblast cells. In the present study, choriocarcinoma JAr cells were found to express LGALS1, -2, -3, -8, -10, and -13 mRNA and at least LGALS1, -3, and -8 protein, as determined by reverse-transcriptase PCR and Western blot, respectively. The galectin mRNA signature of JAr cells thus differed from that of normal first-trimester extravillous trophoblasts. A Matrigel migration assay was also used to investigate and confirm the relevance and effect of LGALS1 on the invasive potential of JAr cells, as observed in other trophoblast models. This modulation in behavior was achieved by specific lectin-glycan binding.
authors.affiliation: Laboratory for Biology of Reproduction, Institute INEP, University of Belgrade, Belgrade, Serbia.
authors.forename: Nikola
authors.initials: N
authors.lastname: Kolundžić
authors.affiliation: Laboratory for Biology of Reproduction, Institute INEP, University of Belgrade, Belgrade, Serbia.
authors.forename: Danica
authors.initials: D
authors.lastname: Ćujić
authors.affiliation: Laboratory for Biology of Reproduction, Institute INEP, University of Belgrade, Belgrade, Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Abu Rabi
authors.affiliation: Laboratory for Biology of Reproduction, Institute INEP, University of Belgrade, Belgrade, Serbia.
authors.forename: Žanka
authors.initials: Ž
authors.lastname: Bojić-Trbojević
authors.affiliation: Department of Biotechnology, Maebashi Institute of Technology, Maebashi, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kadoya
authors.affiliation: Laboratory for Biology of Reproduction, Institute INEP, University of Belgrade, Belgrade, Serbia.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Vićovac
mesh_terms: D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000378:metabolism* / Q000473:pathology; D005260:Female; D037483:Galectin 1 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D009361:Neoplasm Invasiveness; D010929:Placentation; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D037483:Galectin 1