title: AQP1, AQP5, Bcl-2 and p16 in pharyngeal squamous cell carcinoma.
abstract: OBJECTIVE
  This study aimed to link expression patterns of AQP1, AQP5, Bcl-2 and p16 to clinicopathological characteristics of oro-hypopharyngeal squamous cell carcinomas.
  
  
  METHODS
  Immunohistochemical expression of AQP1, AQP5, Bcl-2 and p16 was investigated in 107 consecutive oro-hypopharyngeal squamous cell carcinoma cases. Molecular interrelationship and correlations with clinicopathological parameters and survival were computed.
  
  
  RESULTS
  AQP1 was expressed exclusively by a subgroup of basaloid-like squamous cell carcinomas. AQP5 was detected in 25.2 per cent of the samples, showing significant association with the absence of p16 and Bcl-2 (p = 0.018; p = 0.010). In multivariate analysis, overexpression of p16 was significantly correlated with favourable overall survival (p = 0.014).
  
  
  CONCLUSIONS
  AQP5 defined a subset of patients with Bcl-2-negative and p16-negative tumours with a poor clinical outcome. AQP1 was found to be a marker of a subgroup of aggressive basaloid-like squamous cell carcinomas. These findings suggest that AQP1 and AQP5 are interesting candidates for further studies on risk group classification and personalised treatment of oro-hypopharyngeal squamous cell carcinomas.
authors.affiliation: Department for Oto-Rhino-Laryngology,Head and Neck Surgery,St Anna Clinic,Teaching Hospital of the University of Dusseldorf Medical School,Wuppertal,Germany.
authors.forename: G F
authors.initials: GF
authors.lastname: Lehnerdt
authors.affiliation: Institute of Pharmacogenetics,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: H S
authors.initials: HS
authors.lastname: Bachmann
authors.affiliation: Department of Anesthesiology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: M
authors.initials: M
authors.lastname: Adamzik
authors.affiliation: Institute of Pathology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: A
authors.initials: A
authors.lastname: Panic
authors.affiliation: Institute of Pathology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: E
authors.initials: E
authors.lastname: Köksal
authors.affiliation: Department of Oto-Rhino-Laryngology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: P
authors.initials: P
authors.lastname: Weller
authors.affiliation: Department of Oto-Rhino-Laryngology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: S
authors.initials: S
authors.lastname: Lang
authors.affiliation: Institute of Pathology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: K W
authors.initials: KW
authors.lastname: Schmid
authors.affiliation: Institute of Pharmacogenetics,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: W
authors.initials: W
authors.lastname: Siffert
authors.affiliation: Institute of Pathology,University Hospital Essen,University of Duisburg-Essen,Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bankfalvi
mesh_terms: D051398:Aquaporin 1 / Q000096:biosynthesis / Q000235:genetics*; D051402:Aquaporin 5 / Q000096:biosynthesis / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C496020:AQP1 protein, human; C496036:AQP5 protein, human; D051402:Aquaporin 5; D014408:Biomarkers, Tumor; C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051398:Aquaporin 1
keywords: Aquaporin 1; Aquaporin 5; Bcl2-Antagonist Of Cell Death Protein; Human; P16 Protein; Squamous Cell Carcinoma Of The Head And Neck