title: Amperometric Detection of Aqueous Silver Ions by Inhibition of Glucose Oxidase Immobilized on Nitrogen-Doped Carbon Nanotube Electrodes.
abstract: An amperometric glucose biosensor based on immobilization of glucose oxidase on nitrogen-doped carbon nanotubes (N-CNTs) was successfully developed for the determination of silver ions. Upon exposure to glucose, a steady-state enzymatic turnover rate was detected through amperometric oxidation of the H2O2 byproduct, directly related to the concentration of glucose in solution. Inhibition of the steady-state enzymatic glucose oxidase reaction by heavy metals ions such as Ag(+), produced a quantitative decrease in the steady-state rate, subsequently creating an ultrasensitive metal ion biosensor through enzymatic inhibition. The Ag(+) biosensor displayed a sensitivity of 2.00 × 10(8) ± 0.06 M(-1), a limit of detection (σ = 3) of 0.19 ± 0.04 ppb, a linear range of 20-200 nM, and sample recovery at 101 ± 2%, all acquired at a low-operating potential of 0.05 V (vs Hg/Hg2SO4). Interestingly, the biosensor does not display a loss in sensitivity with continued use due to the % inhibition based detection scheme: loss of enzyme (from continued use) does not influence the % inhibition, only the overall current associated with the activity loss. The heavy metals Cu(2+) and Co(2+) were also detected using the enzyme biosensor but found to be much less inhibitory, with sensitivities of 1.45 × 10(6) ± 0.05 M(-1) and 2.69 × 10(3) ± 0.07 M(-1), respectively. The mode of GOx inhibition was examined for both Ag(+) and Cu(2+) using Dixon and Cornish-Bowden plots, where a strong correlation was observed between the inhibition constants and the biosensor sensitivity.
authors.affiliation: †Department of Chemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St. Stop A5300, Austin, Texas 78712-1224, United States.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Rust
authors.affiliation: †Department of Chemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St. Stop A5300, Austin, Texas 78712-1224, United States.
authors.forename: Jacob M
authors.initials: JM
authors.lastname: Goran
authors.affiliation: †Department of Chemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St. Stop A5300, Austin, Texas 78712-1224, United States.
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Stevenson
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005949:Glucose Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007477:Ions / Q000032:analysis / Q000494:pharmacology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D012834:Silver / Q000032:analysis* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D007477:Ions; D037742:Nanotubes, Carbon; D014867:Water; D012834:Silver; D005949:Glucose Oxidase; D009584:Nitrogen