title: Imprinted Contact Lenses for Sustained Release of Polymyxin B and Related Antimicrobial Peptides.
abstract: The aim of this work was to develop drug-soft contact lens combination products suitable for controlled release of antimicrobial peptides on the ocular surface. Incorporation of functional monomers and the application of molecular imprinting techniques were explored to endow 2-hydroxyethyl methacrylate (HEMA) hydrogels with the ability to load and to sustain the release of polymyxin B and vancomycin. Various HEMA-drug-functional monomer-cross-linker molar ratios were evaluated to prepare polymyxin B imprinted and non-imprinted hydrogels. Acrylic acid-functionalized and imprinted hydrogels loaded greater amounts of polymyxin B and led to more sustained release profiles, in comparison with non-functionalized and non-imprinted networks. Polymyxin B-loaded hydrogels showed good biocompatibility in hen's egg test-chorioallantoic membrane tests. Functionalized hydrogels also loaded vancomycin and sustained its release, but the imprinting effect was only exhibited with polymyxin B, as demonstrated in rebinding tests. Microbiological assays carried out with Pseudomonas aeruginosa allowed identification of the most suitable hydrogel composition for efficient bacteria eradication; some hydrogels being able to stand several continued challenges against this important bacterial pathogen.
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Santiago de Compostela, 15782, Spain.|School of Pharmacy, Boots Science Building, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Negin
authors.initials: N
authors.lastname: Malakooti
authors.affiliation: School of Pharmacy, Boots Science Building, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Cameron
authors.initials: C
authors.lastname: Alexander
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Santiago de Compostela, 15782, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Alvarez-Lorenzo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001682:Biological Availability; D002645:Chickens; D003263:Contact Lenses, Hydrophilic; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems / Q000379:methods*; D020100:Hydrogels; D008689:Methacrylates / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000008:administration & dosage; D011112:Polymyxin B / Q000008:administration & dosage*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D014640:Vancomycin / Q000008:administration & dosage
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D008689:Methacrylates; D010455:Peptides; C005044:hydroxyethyl methacrylate; D014640:Vancomycin; D011112:Polymyxin B
keywords: Pseudomonas aeruginosa; biomaterials; contact lens; controlled release; drug-device combination product; hydrogels; molecular imprinting; mucosal delivery; ophthalmic drug delivery; peptide delivery