title: Molecular characterization of a new human echovirus 11 isolate associated with severe hand, foot and mouth disease in Yunnan, China, in 2010.
abstract: Human echovirus 11 (E-11), a member of the species Enterovirus B, frequently causes aseptic meningitis and hand, foot and mouth disease (HFMD). We determined the complete genome sequence of strain 520K/YN/CHN/2010, isolated from a subject with HFMD and aseptic meningitis in Yunnan Province, China, in 2010. The strain shared 78.8% and 81.1% nucleotide sequence similarity with prototype strain Gregory in the complete VP1 gene and the complete genome, respectively. Only the VP2-VP3-VP1 genome region of 520K/YN/CHN/2010 was similar to that of the E-11 strain; the other genome regions were most similar to those of other members of the species Enterovirus B. Using phylogenetic analysis and sequence comparisons of the complete VP1 gene, E-11 strains could be divided into five genogroups, and the 520K/YN/CHN/2010 strain was found to belong to genogroup A. Recombination analysis showed evidence of recombination with other member of the species Enterovirus B, especially the E-9 strain MSH/KM812/2010. Persistent surveillance of HFMD pathogens might help predict potential emerging viruses and related disease outbreaks.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yanju
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zhengling
authors.initials: Z
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Shunqiao
authors.initials: S
authors.lastname: Yang
authors.forename: Zexu
authors.initials: Z
authors.lastname: Yu
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.forename: Shaohui
authors.initials: S
authors.lastname: Ma
mesh_terms: D002681:China; D016000:Cluster Analysis; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000150:complications / Q000821:virology*; D006801:Humans; D008582:Meningitis, Aseptic / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins