title: Genome-Wide H3K4me3 Analysis in Angus Cattle with Divergent Tenderness.
abstract: Tenderness is one of the most important properties of meat quality, which is influenced by genetic and environmental factors. As an intensively studied epigenetic marker, histone methylation, occurring on arginine and lysine residues, has pivotal regulatory functions on gene expression. To examine whether histone methylation involves in beef tenderness variation, we analyzed the transcriptome and H3K4me3 enrichment profiles of muscle strips obtained from the longissimus dorsi (LD) of Angus steers previously classify to the tender or tough group. We first plotted a global bovine H3K4me3 map on chromosomes and called peak-enriched regions and genes. We found that majorities of H3K4me3 on genes were occupying the first intron and intergenic regions and its maps displayed similar patterns in tender and tough groups, with high H3K4me3 enrichment surrounding the transcription start site (TSS). We also explored the relationship of H3K4me3 and gene expression. The results showed that H3K4me3 enrichment is highly positively correlated with gene expression across the whole genome. Cluster analysis results confirmed the relationship of H3K4me3 enrichment and gene expression. By using a pathway-based approach in genes with H3K4me3 enrichment in promoter regions from the tender cluster, we revealed that those genes involved in the development of different tissues-connective tissue, skeletal and muscular system and functional tissues-; while in tough group those genes engaged in cell death, lipid metabolism and small molecule biochemistry. The results from this study provide a deep insight into understanding of the mechanisms of epigenetic regulations in meat quality and beef tenderness.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China; Department of Animal & Avian Sciences, University of Maryland, College Park, MD, United States of America.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Animal & Avian Sciences, University of Maryland, College Park, MD, United States of America.
authors.forename: José A
authors.initials: JA
authors.lastname: Carrillo
authors.affiliation: Department of Animal & Avian Sciences, University of Maryland, College Park, MD, United States of America.
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
authors.affiliation: Department of Animal & Avian Sciences, University of Maryland, College Park, MD, United States of America.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Updike
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, United States of America.
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Animal & Avian Sciences, University of Maryland, College Park, MD, United States of America.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhan
authors.affiliation: Department of Animal & Avian Sciences, University of Maryland, College Park, MD, United States of America.
authors.forename: Jiuzhou
authors.initials: J
authors.lastname: Song
mesh_terms: D000818:Animals; D002417:Cattle; D020869:Gene Expression Profiling; D023281:Genomics; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008297:Male; D008745:Methylation; D058977:Molecular Sequence Annotation; D018482:Muscle, Skeletal / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D000069466:Red Meat
chemical_list: D006657:Histones; D008239:Lysine