title: Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine.
abstract: OBJECTIVE
  Fat deposition in the liver, which leads to nonalcoholic fatty liver disease is associated with obesity. Taurine (Tau) regulates lipid metabolism, representing a possible nutraceutical agent against obesity and its comorbidities. Here, we investigated whether Tau supplementation prevents hepatic lipid accumulation by regulation of the main hepatic genes involved in de novo lipogenesis and β-oxidation.
  
  
  METHODS
  Male rats received subcutaneous injections of monosodium glutamate (MSG; 4 mg/kg body weight/day) or saline (control group, CTL) during the first 5 days of life. From 21 to 120 days of age, half of each of the MSG and CTL groups received 2.5% Tau in drinking water (CTau and MTau).
  
  
  RESULTS
  MSG-treated rats were normoglycemic, hypertriglyceridemic and insulin resistant (IR). MSG rats also exhibited massive obesity and higher hepatic triglyceride (TG) content. This effect was associated with enhanced gene expression of fatty acid synthase (FASN), but reduced carbohydrate response element-binding protein (ChREBP), microsomal TG transfer protein (MTP) and carnitine palmitoyltransferase (CPT)-1a mRNAs in MSG livers. Tau supplementation decreased whole body fat accumulation and serum TG levels, without altering IR. Tau also normalized hepatic TG content by enhancing ChREBP, MTP, peroxisome proliferator-activated receptor (PPAR)-α, ACO (acyl-CoA oxidase) and CPT-1a gene expressions.
  
  
  CONCLUSIONS
  Therefore, increased hepatic TG deposition in MSG-obese rats is associated with an enhanced FASN, and reduced MTP and CPT-1a genes. Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-α which increases lipid oxidation through ACO and CPT-1a gene expressions.
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil. Electronic address: mlbonfleur@hotmail.com.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Bonfleur
authors.affiliation: Laboratório de Pâncreas Endócrino e Metabolismo, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Patrícia Cristine
authors.initials: PC
authors.lastname: Borck
authors.affiliation: Universidade Federal do Rio de Janeiro (UFRJ), Campus UFRJ-Macaé, Macaé, RJ, Brazil.
authors.forename: Rosane Aparecida
authors.initials: RA
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Caetano
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil.
authors.forename: Gabriela Moreira
authors.initials: GM
authors.lastname: Soares
authors.affiliation: Laboratório de Pâncreas Endócrino e Metabolismo, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Everardo Magalhães
authors.initials: EM
authors.lastname: Carneiro
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil.
authors.forename: Sandra Lucinei
authors.initials: SL
authors.lastname: Balbo
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D005227:Fatty Acids / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009765:Obesity / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D013654:Taurine
keywords: Acyl-CoA oxidase; Carnitine palmitoyltransferase 1a; MSG-obesity; Microsomal TG transfer protein; Monosodium glutamate (PubChem CID: 16219392); Peroxisome proliferator-activated receptor α; Taurine (PubChem CID: 1123); Taurine supplementation