title: Structure of the O-specific polysaccharide from the legume endosymbiotic bacterium Ochrobactrum cytisi strain ESC1(T).
abstract: The O-specific polysaccharide was obtained from the lipopolysaccharide of the legume-endosymbiotic bacterium Ochrobactrum cytisi strain ESC1(T) and studied by chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharide was found to have a disaccharide repeating unit containing α-d-fucose and β-N-acetyl-d-galactosamine residues connected via (1→3)-glycosidic bonds, resulting in the following structure: →3)-α-d-Fucp-(1→3)-β-d-GalpNAc-(1→ The d-GalpNAc residue was nonstoichiometrically substituted with a 4-O-methyl group (∼10%) or with a 4,6-O-(1-carboxy)-ethylidene residue (pyruvyl group) (∼10%).
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Pac
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Komaniecka
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zamlynska
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Turska-Szewczuk
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: adam.choma@poczta.umcs.lublin.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Choma
mesh_terms: D002240:Carbohydrate Sequence; D007887:Fabaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D042041:Ochrobactrum / Q000737:chemistry* / Q000502:physiology*; D013559:Symbiosis
chemical_list: D019081:O Antigens
keywords: Fucose; Galactosamine; Lipopolysaccharide; Ochrobactrum cytisi; Pyruvyl