title: The key microorganisms for anaerobic degradation of pentachlorophenol in paddy soil as revealed by stable isotope probing.
abstract: Pentachlorophenol (PCP) is a common residual persistent pesticide in paddy soil and has resulted in harmful effect on soil ecosystem. The anaerobic microbial transformation of PCP, therefore, has been received much attentions, especially the functional microbial communities for the reductive transformation. However, the key functional microorganisms for PCP mineralization in the paddy soil still remain unknown. In this work, DNA-based stable isotope probing (SIP) was applied to explore the key microorganisms responsible for PCP mineralization in paddy soil. The SIP results indicated that the dominant bacteria responsible for PCP biodegradation belonged to the genus Dechloromonas of the class β-Proteobacteria. In addition, the increased production of (13)CH4 and (13)CO2 indicated that the addition of lactate enhanced the rate of biodegradation and mineralization of PCP. Two archaea classified as the genera of Methanosaeta and Methanocella of class Methanobacteria were enriched in the heavy fraction when with lactate, whereas no archaea was detected in the absence of lactate. These findings provide direct evidence for the species of bacteria and archaea responsible for anaerobic PCP or its breakdown products mineralization and reveal a new insight into the microorganisms linked with PCP degradation in paddy soil.
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China; Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550009, PR China.
authors.forename: Chengshuai
authors.initials: C
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China. Electronic address: cefbli@soil.gd.cn.
authors.forename: Fangbai
authors.initials: F
authors.lastname: Li
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Luo
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Manjia
authors.initials: M
authors.lastname: Chen
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Hu
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D004247:DNA / Q000737:chemistry; D019605:Euryarchaeota / Q000378:metabolism; D019344:Lactic Acid / Q000494:pharmacology; D008697:Methane / Q000378:metabolism; D012275:Oryza; D010416:Pentachlorophenol / Q000737:chemistry / Q000378:metabolism*; D010573:Pesticide Residues / Q000737:chemistry / Q000378:metabolism*; D020560:Proteobacteria / Q000737:chemistry / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D010573:Pesticide Residues; D012989:Soil Pollutants; D002245:Carbon Dioxide; D019344:Lactic Acid; D004247:DNA; D010416:Pentachlorophenol; D008697:Methane
keywords: Archaea; Pentachlorophenol; Stable isotope probing