title: Electrochemiluminescence DNA sensor array for multiplex detection of biowarfare agents.
abstract: Development of a fully automated electrochemiluminescence (ECL) DNA assay for multiplex detection of six biowarfare agents is described. Aminated-DNA capture probes were covalently immobilised on activated-carbon electrodes and subsequently hybridised to target strands. Detection was achieved via a sandwich-type assay after Ru(bpy)3(2+)-labelled reporter probes were hybridised to the formed probe-target complexes. The assay was performed in an automated microsystem in a custom designed ECL detection box with integrated fluidics, electronics,and movable photomultiplier detector. The obtained limits of detection were 0.6-1.2 nmol L(-1) for six targets ranging from 50 to 122 base pairs in size, with linear range 1-15 nmol L(-1). Non-specific adsorption and cross-reactivity were very low. Detection of six targets on a single chip was achieved with subnanomolar detection limits.
authors.affiliation: Nanobiotechnology and Bioanalysis Group, INTERFIBIO Consolidated Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain, annamaria.spehar@gmail.com.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Spehar-Délèze
authors.forename: Rainer
authors.initials: R
authors.lastname: Gransee
authors.forename: Sergio
authors.initials: S
authors.lastname: Martinez-Montequin
authors.forename: Diego
authors.initials: D
authors.lastname: Bejarano-Nosas
authors.forename: Samuel
authors.initials: S
authors.lastname: Dulay
authors.forename: Sandra
authors.initials: S
authors.lastname: Julich
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D054045:Biological Warfare Agents / Q000145:classification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008163:Luminescent Measurements / Q000295:instrumentation*; D046228:Microarray Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D054045:Biological Warfare Agents; D004247:DNA