title: Effects of the association of antifungal drugs on the antimicrobial action of endodontic sealers.
abstract: This in vitro study aimed to determine the susceptibility of oral specimens and ATCC lineages of Candida albicans for five endodontic sealers, which were pure and associated with two antifungal drugs, and to analyze their effect on the physical properties. For this purpose, 30 lineages of C. albicans, collected from the oral cavity of patients assisted at the endodontics clinic of the Universidade Sagrado Coração, were analyzed. Yeasts susceptibility to the sealers was tested by diffusion on agar plates. Physical properties were evaluated according to the ADA specification no. 57. The pure versions of the Sealer 26, AH Plus, Endofill, Fillapex, and Sealapex demonstrated antifungal activity, with Endofill presenting the greatest inhibition zones. All cements, except for Endofill, had their antifungal actions enhanced by addition of ketoconazole and fluconazole (p < 0.05), and the AH Plus presented the best antifungal activity. The addition of antifungal drugs did not interfere with the setting time and flowability of the sealers. It was concluded that the addition of antifungals to endodontic sealers enhanced the antimicrobial action of most cements tested without altering their physical properties.
authors.affiliation: Pro-Rectory of Research and Post Graduation, Universidade Sagrado Coração, Bauru, SP, Brazil.
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Weckwerth
authors.affiliation: Pro-Rectory of Research and Post Graduation, Universidade Sagrado Coração, Bauru, SP, Brazil.
authors.forename: Fellipe Lombardo de Souza
authors.initials: FL
authors.lastname: Lima
authors.affiliation: Pro-Rectory of Research and Post Graduation, Universidade Sagrado Coração, Bauru, SP, Brazil.
authors.forename: Vanessa Raquel
authors.initials: VR
authors.lastname: Greatti
authors.affiliation: Department of Operative Dentistry, Endodontics and Dental Materials, Bauru Dental School, Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Marco Antonio Hungaro
authors.initials: MA
authors.lastname: Duarte
authors.affiliation: Department of Operative Dentistry, Endodontics and Dental Materials, Bauru Dental School, Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Rodrigo Ricci
authors.initials: RR
authors.lastname: Vivan
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001729:Bismuth / Q000737:chemistry / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology; D015725:Fluconazole / Q000737:chemistry / Q000494:pharmacology*; D007654:Ketoconazole / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D015203:Reproducibility of Results; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D012459:Salicylates / Q000737:chemistry / Q000494:pharmacology; D018709:Statistics, Nonparametric
chemical_list: D000935:Antifungal Agents; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; C080620:Sealer 26; C534916:epoxy resin-based root canal sealer; C074417:Endo-Fill; C055601:Sealapex; D015725:Fluconazole; D002126:Calcium Hydroxide; D007654:Ketoconazole; D001729:Bismuth