title: High Throughput Screening and Selection Methods for Directed Enzyme Evolution.
abstract: Successful evolutionary enzyme engineering requires a high throughput screening or selection method, which considerably increases the chance of obtaining desired properties and reduces the time and cost. In this review, a series of high throughput screening and selection methods are illustrated with significant and recent examples. These high throughput strategies are also discussed with an emphasis on compatibility with phenotypic analysis during directed enzyme evolution. Lastly, certain limitations of current methods, as well as future developments, are briefly summarized.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Department of Biochemistry, and Departments of Chemistry and Bioengineering and Institute for Genomic Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Han
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Chemical and Biomolecular Engineering, Department of Biochemistry, and Departments of Chemistry and Bioengineering and Institute for Genomic Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Department of Chemical and Biomolecular Engineering, Department of Biochemistry, and Departments of Chemistry and Bioengineering and Institute for Genomic Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao