title: Mannose-centered aromatic galactoclusters inhibit the biofilm formation of Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa (PA) is a major public health care issue due to its ability to develop antibiotic resistance mainly through adhesion and biofilm formation. Therefore, targeting the bacterial molecular arsenal involved in its adhesion and the formation of its biofilm appears as a promising tool against this pathogen. The galactose-binding LecA (or PA-IL) has been described as one of the PA virulence factors involved in these processes. Herein, the affinity of three tetravalent mannose-centered galactoclusters toward LecA was evaluated with five different bioanalytical methods: HIA, ELLA, SPR, ITC and DNA-based glycoarray. Inhibitory potential towards biofilms was then assessed for the two glycoclusters with highest affinity towards LecA (Kd values of 157 and 194 nM from ITC measurements). An inhibition of biofilm formation of 40% was found for these galactoclusters at 10 μM concentration. Applications of these macromolecules in anti-bacterial therapy are therefore possible through an anti-adhesive strategy.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université de Montpellier, ENSCM, place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France. morvan@univ-montp2.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: Ligeour
authors.forename: Olivier
authors.initials: O
authors.lastname: Vidal
authors.forename: Lucie
authors.initials: L
authors.lastname: Dupin
authors.forename: Francesca
authors.initials: F
authors.lastname: Casoni
authors.forename: Emilie
authors.initials: E
authors.lastname: Gillon
authors.forename: Albert
authors.initials: A
authors.lastname: Meyer
authors.forename: Sébastien
authors.initials: S
authors.lastname: Vidal
authors.forename: Gérard
authors.initials: G
authors.lastname: Vergoten
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lacroix
authors.forename: Eliane
authors.initials: E
authors.lastname: Souteyrand
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.forename: François
authors.initials: F
authors.lastname: Morvan
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D005690:Galactose / Q000737:chemistry* / Q000494:pharmacology*; D008358:Mannose / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*
chemical_list: D008358:Mannose; D005690:Galactose