title: A Putative Type III Secretion System Effector Encoded by the MA20_12780 Gene in Bradyrhizobium japonicum Is-34 Causes Incompatibility with Rj4 Genotype Soybeans.
abstract: The nodulation of Bradyrhizobium japonicum Is-34 is restricted by Rj4 genotype soybeans (Glycine max). To identify the genes responsible for this incompatibility, Tn5 mutants of B. japonicum Is-34 that were able to overcome this nodulation restriction were obtained. Analysis of the Tn5 mutants revealed that Tn5 was inserted into a region containing the MA20_12780 gene. In addition, direct disruption of this gene using marker exchange overcame the nodulation restriction by Rj4 genotype soybeans. The MA20_12780 gene has a tts box motif in its upstream region, indicating a possibility that this gene encodes a type III secretion system (T3SS) effector protein. Bioinformatic characterization revealed that the MA20_12780 protein contains the small ubiquitin-like modifier (SUMO) protease domain of the C48 peptidase (ubiquitin-like protease 1 [Ulp1]) family. The results of the present study indicate that a putative T3SS effector encoded by the MA20_12780 gene causes the incompatibility with Rj4 genotype soybeans, and they suggest the possibility that the nodulation restriction of B. japonicum Is-34 may be due to Rj4 genotype soybeans recognizing the putative T3SS effector (MA20_12780 protein) as a virulence factor.
authors.affiliation: Graduate School of Life Science, Tohoku University, Miyagi, Japan Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan tsurumaru@ige.tohoku.ac.jp.
authors.forename: Hirohito
authors.initials: H
authors.lastname: Tsurumaru
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Syougo
authors.initials: S
authors.lastname: Hashimoto
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Okizaki
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Tokyo, Japan Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.affiliation: Department of Biosciences and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Yamakawa
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020369:Bradyrhizobium / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D055170:Plant Root Nodulation; D018517:Plant Roots / Q000382:microbiology; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics / Q000382:microbiology*; D000069296:Type III Secretion Systems / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D000069296:Type III Secretion Systems