title: Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.
abstract: This paper illustrates the possibility of measuring chlorophyll (Chl) content and Chl fluorescence parameters by the solar-induced Chl fluorescence (SIF) method using the Fraunhofer line depth (FLD) principle, and compares the results with the standard measurement methods. A high-spectral resolution HR2000+ and an ordinary USB4000 spectrometer were used to measure leaf reflectance under solar and artificial light, respectively, to estimate Chl fluorescence. Using leaves of Capsicum annuum cv. 'Sven' (paprika), the relationships between the Chl content and the steady-state Chl fluorescence near oxygen absorption bands of O2B (686nm) and O2A (760nm), measured under artificial and solar light at different growing stages of leaves, were evaluated. The Chl fluorescence yields of ΦF 686nm/ΦF 760nm ratios obtained from both methods correlated well with the Chl content (steady-state solar light: R(2) = 0.73; artificial light: R(2) = 0.94). The SIF method was less accurate for Chl content estimation when Chl content was high. The steady-state solar-induced Chl fluorescence yield ratio correlated very well with the artificial-light-induced one (R(2) = 0.84). A new methodology is then presented to estimate photochemical yield of photosystem II (ΦPSII) from the SIF measurements, which was verified against the standard Chl fluorescence measurement method (pulse-amplitude modulated method). The high coefficient of determination (R(2) = 0.74) between the ΦPSII of the two methods shows that photosynthesis process parameters can be successfully estimated using the presented methodology.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.
authors.forename: Bayaer
authors.initials: B
authors.lastname: Tubuxin
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan School of Forest Resources, The University of Maine, 5557 Nutting Hall, Orono, ME 04469, USA.
authors.forename: Parinaz
authors.initials: P
authors.lastname: Rahimzadeh-Bajgiran
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Ginnan
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan.
authors.forename: Fumiki
authors.initials: F
authors.lastname: Hosoi
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 Japan aomasa@mail.ecc.u-tokyo.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Omasa
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D045332:Photosystem II Protein Complex; D002734:Chlorophyll
keywords: Chlorophyll content; Fraunhofer line depth principle; different leaf age; photochemical yield of photosystem II (ΦPSII); solar-induced chlorophyll fluorescence.