title: Protection against Amoebic Liver Abscess in Hamster by Intramuscular Immunization with an Autographa californica Baculovirus Driving the Expression of the Gal-Lectin LC3 Fragment.
abstract: In a previous study, we demonstrated that oral immunization using Autographa californica baculovirus driving the expression of the Gal-lectin LC3 fragment (AcNPV-LC3) of Entamoeba histolytica conferred protection against ALA development in hamsters. In this study, we determined the ability of AcNPV-LC3 to protect against ALA by the intramuscular route as well as the liver immune response associated with protection. Results showed that 55% of hamsters IM immunized with AcNPV-LC3 showed sterile protection against ALA, whereas other 20% showed reduction in the size and extent of abscesses, resulting in some protection in 75% of animals compared to the sham control group. Levels of protection showed a linear correlation with the development and intensity of specific antiamoeba cellular and humoral responses, evaluated in serum and spleen of hamsters, respectively. Evaluation of the Th1/Th2 cytokine patterns expressed in the liver of hamsters showed that sterile protection was associated with the production of high levels of IFNγ and IL-4. These results suggest that the baculovirus system is equally efficient by the intramuscular as well as the oral routes for ALA protection and that the Gal-lectin LC3 fragment is a highly protective antigen against hepatic amoebiasis through the local induction of IFNγ and IL-4.
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Dulce María
authors.initials: DM
authors.lastname: Meneses-Ruiz
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Hugo
authors.initials: H
authors.lastname: Aguilar-Diaz
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Raúl José
authors.initials: RJ
authors.lastname: Bobes
authors.affiliation: Departamento de Biología Celular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Sampieri
authors.affiliation: Departamento de Biología Celular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Luis
authors.identifier: 0000-0003-2266-4847
authors.initials: L
authors.lastname: Vaca
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Laclette
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, Mexico.
authors.forename: Julio César
authors.initials: JC
authors.lastname: Carrero
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D016367:Baculoviridae / Q000276:immunology*; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000187:drug effects / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G; D008101:Liver Abscess, Amebic / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D016052:Protozoan Vaccines