title: PRDM1, a Tumor-Suppressor Gene, is Induced by Genkwadaphnin in Human Colon Cancer SW620 Cells.
abstract: Genkwadaphnin (GD-1) is isolated from the flower buds of Daphne genkwa Siebold et Zuccarini (Thymelaeaceae), and it has been used as a traditional Korean and Chinese medicine. In this study, the authors observe that GD-1 inhibits the growth of the colon cancer cell line, SW620, through the up-regulation of p21 expression in a PRDM1-dependent manner. After treatment with GD-1, the transcriptional repressor PRDM1 is prominently induced in SW620 cells. Furthermore, GD-1 induce the phosphorylation of PKD1 and MEK and subsequently provide PRDM1 enhancement, resulting in the suppression of c-Myc expression and the up-regulation of p21. PKD1 knockdown using siRNA abrogates PRDM1 expression by GD-1 and subsequently disrupts the regulation of c-Myc and p21 expression. Treating SW620 cells with GD-1 inhibits cell-cycle progression and is characterized by the down-regulation of c-Myc followed by the up-regulation of p21 expression. The up-regulation of p21 by GD-1 induces the growth arrest of the SW620 colon cancer cell line. Based on these data, the authors propose that GD-1 has tumor-suppressor activity that may contribute to the anti-tumor effects of PRDM1 in colon cancer.
authors.affiliation: Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Ho-Bum
authors.initials: HB
authors.lastname: Kang
authors.affiliation: Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Ha-Reum
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Da Jung
authors.initials: da J
authors.lastname: Jee
authors.affiliation: Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.|Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Head of Rheumatology, Department of Internal Medicine, Soonchunhyang University Choenan hospital College of Medicine, Choenan, 330-721, Republic of Korea.
authors.forename: Seong-Su
authors.initials: SS
authors.lastname: Nah
authors.affiliation: ENZYCHEM Lifesciences, 103-6, KAIST-ICC F741, Munjidong, Daejeon, 305-732, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Yoon
authors.affiliation: Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Jae Wha
authors.initials: JW
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D004224:Diterpenes; D012097:Repressor Proteins; C087042:PRDM1 protein, human; C036412:genkwadaphnin; D000074462:Positive Regulatory Domain I-Binding Factor 1
keywords: GENKWADAPHNIN (GD-1); PKD1; PRDM1