title: Statistical-based approach in potential diagnostic application of urinary nucleosides in urogenital tract cancer.
abstract: OBJECTIVE
  We aimed at evaluation the potential diagnostic role of urinary nucleosides in urogenital tract cancer.
  
  
  METHODS
  Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses.
  
  
  RESULTS
  We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78-12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78-16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome.
  
  
  CONCLUSIONS
  Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in urogenital tract cancer are likely to occur.
authors.affiliation: Department of Biopharmaceutics & Pharmacodynamics, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland.|Department of Toxicology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, M. Curie-Sklodowskiej 9, 85-094 Bydgoszcz, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Daghir-Wojtkowiak
authors.affiliation: Department of Biopharmaceutics & Pharmacodynamics, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Wiktoria
authors.initials: W
authors.lastname: Struck-Lewicka
authors.affiliation: Department of Biopharmaceutics & Pharmacodynamics, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Waszczuk-Jankowska
authors.affiliation: Department of Urology, Medical University of Gdańsk, Smoluchowskiego 17, 80-214 Gdańsk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Markuszewski
authors.affiliation: Department of Biopharmaceutics & Pharmacodynamics, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Kaliszan
authors.affiliation: Department of Biopharmaceutics & Pharmacodynamics, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Michal Jan
authors.initials: MJ
authors.lastname: Markuszewski
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009705:Nucleosides / Q000652:urine*; D012727:Sex Characteristics; D014565:Urogenital Neoplasms / Q000175:diagnosis* / Q000652:urine*
chemical_list: D014408:Biomarkers, Tumor; D009705:Nucleosides
keywords: biomarkers; biostatistics; diagnostic potential; metabolomics; nucleoside profiles; urogenital cancer