title: Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis.
abstract: BACKGROUND
  Comprehensive analysis of proteome differentially expressed in response to surgery or drug treatment is useful to understand biological responses to dispensed interventions. Here we investigated expression changes in sera of patients who suffered from calcific aortic stenosis (CAS), before and after surgery for aortic valve replacement.
  
  
  METHODS
  Sera obtained before and after surgery with depletion of highly abundant proteins were analyzed with iTRAQ labeling followed by nanoLC-MALDI-TOF/TOF-MS/MS.
  
  
  RESULTS
  Fifty-one proteins shared in five patients were identified with differential levels in postsurgical and presurgical sera. Finally, 16 proteins that show statistically significant levels in patients' sera compared with those in control sera (P < 0.05) were identified. Most of the identified proteins were positive acute-phase proteins. Among three proteins other than acute-phase proteins, we confirmed increased levels of antithrombin-III and zinc-α-2-glycoprotein in postsurgical sera by Western blot analysis using other CAS patients' sera. Furthermore, antithrombin-III and zinc-α-2-glycoprotein were not found among proteins with differential levels in postsurgical and presurgical sera of patients with aortic aneurysms that we identified in a previous study.
  
  
  CONCLUSIONS
  The results indicated that antithrombin-III and zinc-α-2-glycoprotein would become unique monitoring proteins for evaluating pathophysiological and biochemical processes occurring before and after surgery for CAS.
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Satoh
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo, Shimane 693-8501, Japan ; Department of Biochemistry, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Maniwa
authors.affiliation: Division of Cardiovascular and Thoracic Surgery, Department of Surgery, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Teiji
authors.initials: T
authors.lastname: Oda
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism; D054392:Adipokines; D000368:Aged; D000990:Antithrombin III / Q000378:metabolism*; D001021:Aortic Valve / Q000473:pathology* / Q000601:surgery; D001024:Aortic Valve Stenosis / Q000097:blood / Q000601:surgery*; D015415:Biomarkers / Q000097:blood*; D002114:Calcinosis / Q000097:blood / Q000601:surgery*; D002352:Carrier Proteins / Q000097:blood*; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008297:Male; D020543:Proteome / Q000378:metabolism*; D065467:Transcatheter Aortic Valve Replacement; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D000209:Acute-Phase Proteins; D054392:Adipokines; D000990:Antithrombin III; D015415:Biomarkers; D002352:Carrier Proteins; D006023:Glycoproteins; D020543:Proteome; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein