title: Large palpable ductal carcinoma in situ is Her-2 positive with high nuclear grade.
abstract: Ductal carcinoma in situ (DCIS) of the breast is a heterogeneous group with variable clinical presentation. The exact molecular mechanism is not known why some ductal carcinomas may reach to such a large size but still remains in situ. Although, molecular classification of DCIS lesions and nuclear grading are important for identification of more aggressive lesions but it is not sufficient. Our aim was to examine the expression pattern of immunohistochemical (IHC) markers of ER, PR, HER-2 in palpable DCIS lesions and compare with clinicopathological findings. Our center is referral hospital from South of Iran. Samples were obtained from fifty four patients with a diagnosis of palpable DCIS. Equivocal (2+) case in HER-2 IHC testing was more characterized by chromogenic in situ hybridization. The positive frequency of HER2, ER, and PR was 92%, 48%, and 37% respectively. Palpable DCIS lesions were significantly more HER-2 positive (92%). The DCIS cases were more likely to be of high nuclear grade (grade III) and Her-2 positive cases were more likely to be of high nuclear grade than intermediate grade. All ER negative tumors had high nuclear grade. The Her-2 positivity is suggested as the most important factor responsible for marked in situ proliferation and production of palpable mass.
authors.affiliation: Department of Pathology, Hematology Research Center, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Monabati
authors.affiliation: Department of Pathology, Hematology Research Center, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Ali-Reza
authors.initials: AR
authors.lastname: Sokouti
authors.affiliation: Department of Pathology, Hematology Research Center, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Sadat Noori
authors.initials: SN
authors.lastname: Noori
authors.affiliation: Department of Pathology, Hematology Research Center, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Safaei
authors.affiliation: Department of Surgery, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Abd-Rasul
authors.initials: AR
authors.lastname: Talei
authors.affiliation: Department of Radiotherapy, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Shapoor
authors.initials: S
authors.lastname: Omidvari
authors.affiliation: Transplant Research Center, Shiraz University of Medical Sciences Shiraz, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Azarpira
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008875:Middle Aged; D010173:Palpation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human
keywords: Ductal carcinoma in situ; Her-2; grading; palpable