title: Neuroprotective Effect of Radix Trichosanthis Saponins on Subarachnoid Hemorrhage.
abstract: Redox homeostasis has been implicated in subarachnoid hemorrhage (SAH). As a result, antioxidants and/or free radical scavengers have become an important therapeutic modality. Considering that radix trichosanthis (RT) saponins exhibited strong antioxidant ability both in vivo and in vitro, the present study aimed to reveal whether the neuroprotective activities of RT saponins were mediated by p38/p53 signal pathway after SAH. An established SAH model was used and superoxide dismutase (SOD), malondialdehyde (MDA), induced nitric oxide synthase (iNOS), nitric oxide (NO), lactate dehydrogenase (LDH), p-p38, and p53 activation were detected after 48 h of SAH. The results showed that RT saponins inhibited iNOS expression to restore NO to basal level. Moreover, compared with Cu/Zn-SOD, RT saponins (2 mg/kg/d dosage) significantly increased Mn-SOD activity after SAH. Accompanied with lowered NO and elevated SOD, decreased p38 phosphorylation and p53 activities were observed, especially for RT saponins at 2 mg/kg/d dosage. In this setting, the neurological outcome was also improved with less neuronal cells damage after RT saponins pretreatment. Our findings demonstrated the beneficial effects of RT saponins in enhancing neuroprotective effects by deducing iNOS activity, normalizing SOD level, and inhibiting p-p38 and p53 expression, hence offering significant therapeutic implications for SAH.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China.
authors.forename: Liyong
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China.
authors.forename: Juxiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China.
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhou
authors.affiliation: School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Chang