title: The J-domain of heat shock protein 40 can enhance the transduction efficiency of arginine-rich cell-penetrating peptides.
abstract: Sense and antisense oligonucleotide pairs encoding cell-penetrating peptides PTD (Tat47-57), DPV3A, E162, pVEC, R11, and TP13 were used to construct two sets of pET22b-CPP-DsRed and pET22b-CPP-J-DsRed vectors for CPP-DsRed and CPP-J-DsRed recombinant proteins expression. PTD-DsRed, DPV3A-DsRed, PTD-J-DsRed, and DPV3A-J-DsRed recombinant proteins were expressed in a soluble form. PTD-J-DsRed and DPV3A-J-DsRed recombinant proteins were able to escape from E. coli host cells into the culture medium. The membrane-penetrating activity of PTD-J-DsRed and DPV3A-J-DsRed recombinant proteins to mammalian cells was more effective than that of PTD-DsRed and DPV3A-DsRed. The route of the cellular membrane translocation of these recombinant proteins is suggested via macropinocytosis followed by an endosomal escape pathway.
authors.affiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, No. 1, Lane 51, Dahu Road, Xiangshan District, Hsinchu 30093, Taiwan.
authors.forename: Tzu-Yin
authors.initials: TY
authors.lastname: Lin
authors.affiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, No. 1, Lane 51, Dahu Road, Xiangshan District, Hsinchu 30093, Taiwan.
authors.forename: Yu-Hsiu
authors.initials: YH
authors.lastname: Su
authors.affiliation: Division of Biotechnology, Animal Technology Institute Taiwan, No. 52, Kedong 2nd Road, Chunan, Miaoli 35059, Taiwan.
authors.forename: Kun-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, No. 1, Lane 51, Dahu Road, Xiangshan District, Hsinchu 30093, Taiwan.
authors.forename: Chin-Kai
authors.initials: CK
authors.lastname: Chuang
mesh_terms: D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000494:pharmacology; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D017434:Protein Structure, Tertiary; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D057846:Cell-Penetrating Peptides; D050956:HSP40 Heat-Shock Proteins