title: Predictive and prognostic significance of circulating endothelial cells in advanced non-small cell lung cancer patients.
abstract: The aim of this study was to evaluate the predictive and prognostic values of circulating endothelial cells (CECs) in patients with advanced non-small cell lung cancer (NSCLC). A total of 102 newly diagnosed advanced NSCLC patients were enrolled in this study. The amount of CECs was enumerated by flow cytometry (CD45- CD31+ CD146+) at baseline. CEC counts of 56 patients were detected before and after two cycles of chemotherapy. We correlated the baseline and reduction of CECs after therapy with objective response rate (ORR), progression-free survival (PFS), and overall survival (OS). The CEC level was significantly higher in advanced NSCLC patients, ranging from 57 to 1300 cells/10(5) cells (mean ± SD = 299 ± 221 cells/10(5) cells), than in patients with benign lesions (205 ± 97 cells/10(5) cells) and healthy volunteers (117 ± 33 cells/10(5) cells). When the cutoff value of CEC counts was 210 cells/10(5) cells, there was no significant association between CEC counts and OR/PFS/OS of the enrolled patients. However, patients with CEC response after chemotherapy have more chances to achieve OR (P < 0.001), and such patients showed longer PFS (P = 0.048) and OS (P = 0.018) than those without CEC response. In the multivariate analysis, the independent prognostic roles of brain metastasis (HR 6.165, P = 0.001), and CEC response (HR 0.442, P = 0.044) were found. The CEC counts could be considered as diagnostic biomarker for advanced NSCLC patients. And the reduction of CECs after treatment might be more ideal than the baseline CEC counts as a predictive or prognostic factor in patients treated with chemotherapy or anti-angiogenic therapy.
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Yuan
authors.affiliation: Department of Gynecology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Nanjing Second Hospital affiliated to Medical College, Southeast University, Nanjing, 210003, China.
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Lv
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, China.
authors.forename: Xing-qun
authors.initials: XQ
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, China.
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, China. yong_song6310@yahoo.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Song
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood*; D051929:CD146 Antigen / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000473:pathology; D002452:Cell Count; D019070:Cell Lineage; D018572:Disease-Free Survival; D042783:Endothelial Cells / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000235:genetics; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D011379:Prognosis
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D014408:Biomarkers, Tumor; D051929:CD146 Antigen; C497902:MCAM protein, human; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human
keywords: Anti-angiogenic therapy; Circulating endothelial cells; Non-small cell lung cancer; Predictive factor; Prognostic factor