title: Detection of Human α-L-Fucosidases by a Quinone Methide-Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection.
abstract: α-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human α-L-fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor.
authors.affiliation: Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program and the Institute of Biological Chemistry, Academia Sinica, No.128, Academia Road Section 2, Nan-Kang, Taipei 11529 (Taiwan).|Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan).
authors.forename: Manjula
authors.initials: M
authors.lastname: Nandakumar
authors.affiliation: Department of Chemistry, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan).
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Hsu
authors.affiliation: Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program and the Institute of Biological Chemistry, Academia Sinica, No.128, Academia Road Section 2, Nan-Kang, Taipei 11529 (Taiwan).|National Tsing-Hua University, 101 Kuang-Fu Road Section 2, Hsinchu 30013 (Taiwan).
authors.forename: Jason C-Y
authors.initials: JC
authors.lastname: Lin
authors.affiliation: Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan).|Department of Chemistry, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan).
authors.forename: Chun
authors.initials: C
authors.lastname: Lo
authors.affiliation: Department of Chemistry, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan). lclo@ntu.edu.tw.
authors.forename: Lee-Chiang
authors.initials: LC
authors.lastname: Lo
authors.affiliation: Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program and the Institute of Biological Chemistry, Academia Sinica, No.128, Academia Road Section 2, Nan-Kang, Taipei 11529 (Taiwan). chunhung@gate.sinica.edu.tw.|Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan). chunhung@gate.sinica.edu.tw.|Department of Chemistry, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan). chunhung@gate.sinica.edu.tw.
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Lin
mesh_terms: D045744:Cell Line, Tumor; D004848:Epithelium / Q000382:microbiology; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D045563:Indolequinones / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D015335:Molecular Probes / Q000378:metabolism*; D013270:Stomach / Q000382:microbiology; D005644:alpha-L-Fucosidase / Q000378:metabolism*
chemical_list: D045563:Indolequinones; D008070:Lipopolysaccharides; D015335:Molecular Probes; C068040:quinone methide; D005644:alpha-L-Fucosidase
keywords: Helicobacter pylori; activity-based probes; fucosidase; lipopolysaccharides; quinone methide