title: SPLUNC1 Is a Significant Marker in Pleural Effusion from Lung Cancer Compared to Tuberculosis.
abstract: SPLUNC1 (Short palate, lung and nasal epithelium clone1) protein is an abundant secretory product of epithelia present throughout the conducting airways. Although its function is still not fully known, most studies have focused on its defensive effect in the infection of human airways and its potential to serve as a molecular marker for lung cancer. In this study, we further evaluated the SPLUNC1 expression in patients with lung disease to explore its role in cancer or tuberculosis at the protein level. We generated a panel of antibodies by using protein from a eukaryotic expression system as the immunogen to mice. It was the panel of SPLUNC1 monoclonal antibodies that allowed us to comparatively determine SPLUNC1 protein in lung cancer and tuberculosis infection by detecting sera and pleural effusion other than airway surface. The results showed that the SPLUNC1 level was not significantly changed either from sera of lung cancer or control. There was a significant increase in pleural effusion from lung cancer when compared to tuberculosis. These results indicate that SPLUNC1 may be a useful marker for tracing lung cancer cells, based on its epithelial origin property in pleural effusion.
authors.affiliation: 1 Department of Endocrinology, Jingzhou Clinical Medical College, Yangtze University , Jingzhou, People's Republic of China .
authors.forename: Jun L
authors.initials: JL
authors.lastname: Xue
authors.affiliation: 2 Department of Central Laboratory, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute , Beijing, People's Republic of China .
authors.forename: Ling
authors.initials: L
authors.lastname: Yi
authors.affiliation: 2 Department of Central Laboratory, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute , Beijing, People's Republic of China .
authors.forename: Zhou H
authors.initials: ZH
authors.lastname: Yan
authors.affiliation: 3 Hebei Chest Hospital , Shijiazhuang, People's Republic of China .
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: 2 Department of Central Laboratory, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute , Beijing, People's Republic of China .
authors.forename: Xiao J
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: 2 Department of Central Laboratory, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute , Beijing, People's Republic of China .
authors.forename: Pang J
authors.initials: PJ
authors.lastname: Wei
authors.affiliation: 1 Department of Endocrinology, Jingzhou Clinical Medical College, Yangtze University , Jingzhou, People's Republic of China .
authors.forename: Jiao E
authors.initials: JE
authors.lastname: Zeng
authors.affiliation: 4 Chinese Center for Disease Control and Prevention , Beijing, People's Republic of China .
authors.forename: Yan L
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: 2 Department of Central Laboratory, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute , Beijing, People's Republic of China .
authors.forename: Hong T
authors.initials: HT
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015415:Biomarkers / Q000097:blood*; D018287:Carcinoma, Large Cell / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006023:Glycoproteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D010750:Phosphoproteins / Q000097:blood* / Q000276:immunology; D010996:Pleural Effusion / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011379:Prognosis; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C485599:BPIFA1 protein, human; D015415:Biomarkers; D006023:Glycoproteins; D010750:Phosphoproteins