title: Inhibitory effect of 5F on development of lung cancer in A/J mice.
abstract: The purpose of the study is to investigate the effect of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) on the model of induced A/J mice lung cancer in A/J mice. The expressions of tumor-related molecules including P65 and Bcl-2 at protein level were examined using the immunohistochemical method (IHC). Side effects of 5F were also monitored. The results indicated that 5F significantly suppressed the development of B[a]P and NNK-induced lung cancer in vivo by facilitating cell apoptosis with minimal side effects. Compared to the expressions of P65 and Bcl-2 in model group, the levels were strongly attenuated both in blank and 5F injection groups. Moreover, P65 and Bcl-2 levels varied among different groups receiving 5F treatment. The expressions of P65 and Bcl-2 were much lower in groups receiving high-concentration 5F treatment than those with low-concentration 5F injection. Findings revealed that 5F inhibited the pathogenesis of lung cancer through accelerating apoptosis in a dose-dependent manner.
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Yingnian
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College Zhanjiang 524023, Guangdong Province, China.
authors.forename: Xuebao
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001564:Benzo(a)pyrene; D045786:Diterpenes, Kaurane / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008175:Lung Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009602:Nitrosamines; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; D009602:Nitrosamines; D019253:Proto-Oncogene Proteins c-bcl-2; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; C587480:ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid; C052617:Bcl2 protein, mouse; D001564:Benzo(a)pyrene; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone
keywords: 5F; A/J mice; induced lung cancer; suppression