title: Synthesis, Characterization, and Application in HeLa Cells of an NIR Light Responsive Doxorubicin Delivery System Based on NaYF4:Yb,Tm@SiO2-PEG Nanoparticles.
abstract: Herein, we present a phototriggered drug delivery system based on light responsive nanoparticles, which is able to release doxorubicin upon NIR light illumination. The proposed system is based on upconversion fluorescence nanoparticles of β-NaYF4:Yb,Tm@SiO2-PEG with a mean diameter of 52±2.5 nm that absorb the NIR light and emit UV light. The UV radiation causes the degradation of photodegradable ortho-nitrobenzyl alcohol derivates, which are attached on one side to the surface of the nanoparticles and on the other to doxorubicin. This degradation triggers the doxorubicin release. This drug delivery system has been tested "in vitro" with HeLa cells. The results of this study demonstrated that this system caused negligible cytotoxicity when they were not illuminated with NIR light. In contrast, under NIR light illumination, the HeLa cell viability was conspicuously reduced. These results demonstrated the suitability of the proposed system to control the release of doxorubicin via an external NIR light stimulus.
authors.affiliation: †Department of Physical-Chemistry II, Pharmacy Faculty, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Paulino
authors.initials: P
authors.lastname: Alonso-Cristobal
authors.affiliation: †Department of Physical-Chemistry II, Pharmacy Faculty, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Olalla
authors.initials: O
authors.lastname: Oton-Fernandez
authors.affiliation: †Department of Physical-Chemistry II, Pharmacy Faculty, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Mendez-Gonzalez
authors.affiliation: ‡Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: J Fernando
authors.initials: JF
authors.lastname: Díaz
authors.affiliation: †Department of Physical-Chemistry II, Pharmacy Faculty, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Lopez-Cabarcos
authors.affiliation: ‡Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Barasoain
authors.affiliation: †Department of Physical-Chemistry II, Pharmacy Faculty, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Rubio-Retama
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis*; D004058:Diffusion; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D053769:Nanocapsules / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000528:radiation effects; D015019:Yttrium / Q000737:chemistry / Q000528:radiation effects
chemical_list: D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D053769:Nanocapsules; C525362:sodium yttriumtetrafluoride; D011092:Polyethylene Glycols; D015019:Yttrium; D004317:Doxorubicin; D005459:Fluorides
keywords: NIR light; core@shell; doxorubicin; phototriggered drug delivery; upconversion