title: Study on Wangzaozin-A-Inducing Cancer Apoptosis and Its Theoretical Protein Targets.
abstract: Wangzaozin A is the most representative cytotoxic C-20-nonoxide compound of Isodon plants of Labiatae. The protein targets of the 2 isomers (X and X') of Wangzaozin A are, respectively, extracted from target fishing dock Web site. Each isomer has 23 targets with good energy scores. The binding modes of each isomer and its targets are, respectively, analyzed by Dock. The energy score of the binding mode between the isomer X and the inositol-1(or 4)-monophosphatase is the smallest. The apoptosis, antiproliferative, and lethal effects of human gastric cancer cells induced by Wangzaozin A are assessed by trypan blue exclusion, hoechst33258 stain in SGC-7901, and flow cytometric measurement. The mechanism of Wangzaozin-A-inducing cancer apoptosis is analyzed. Wangzaozin A inhibits the growth of human gastric cancer SGC-7901 cell lines at the lower concentration (<4.0 µmol/L) and results in the lethal effect at the relative higher concentration (>8.0 µmol/L).
authors.affiliation: Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, China jchen@nwnu.edu.cn luxq@nwnu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, China.
authors.forename: Shixia
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, China jchen@nwnu.edu.cn luxq@nwnu.edu.cn.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051357:Cyclin-Dependent Kinase 2 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D051357:Cyclin-Dependent Kinase 2
keywords: Wangzaozin A; apoptosis; cancer; docking; molecular dynamics