title: Development of a bread delivery vehicle for dietary prebiotics to enhance food functionality targeted at those with metabolic syndrome.
abstract: Prebiotics are dietary carbohydrates that favourably modulate the gut microbiota. The aims of the present study were to develop a functional prebiotic bread using Bimuno®, (galactooligosaccharide (B-GOS) mixture), for modulation of the gut microbiota in vitro in individuals at risk of metabolic syndrome. A control bread, (no added prebiotic) and positive control bread (containing equivalent carbohydrate to B-GOS bread) were also developed. A 3-stage continuous in vitro colonic model was used to assess prebiotic functionality of the breads. Bacteria were quantified by fluorescence in situ hybridization and short chain fatty acids by gas chromatography. Ion-exchange chromatography was used to determine GOS concentration after bread production. Following B-GOS bread fermentation numbers of bifidobacteria and lactobacilli were significantly higher compared to controls. There was no significant degradation of B-GOS during bread manufacture, indicating GOS withstood the manufacturing process. Furthermore, based on previous research, increased bifidobacteria and butyrate levels could be of benefit to those with obesity related conditions. Our findings support utilization of prebiotic enriched bread for improving gastrointestinal health.
authors.affiliation: a Department of Food and Nutritional Sciences ; University of Reading , Reading , UK.
authors.forename: Adele
authors.initials: A
authors.lastname: Costabile
authors.affiliation: a Department of Food and Nutritional Sciences ; University of Reading , Reading , UK.
authors.forename: Gemma E
authors.initials: GE
authors.lastname: Walton
authors.affiliation: b Clasado Ltd ; Milton Keynes , UK.
authors.forename: George
authors.initials: G
authors.lastname: Tzortzis
authors.affiliation: b Clasado Ltd ; Milton Keynes , UK.
authors.forename: Jelena
authors.initials: J
authors.lastname: Vulevic
authors.affiliation: a Department of Food and Nutritional Sciences ; University of Reading , Reading , UK.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Charalampopoulos
authors.affiliation: a Department of Food and Nutritional Sciences ; University of Reading , Reading , UK.
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Gibson
mesh_terms: D058491:Bacterial Load; D001644:Bifidobacterium / Q000302:isolation & purification; D001939:Bread; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D003106:Colon / Q000737:chemistry* / Q000382:microbiology*; D004035:Diet Therapy / Q000379:methods*; D004337:Drug Carriers / Q000008:administration & dosage*; D005232:Fatty Acids, Volatile / Q000032:analysis; D017404:In Situ Hybridization, Fluorescence; D007778:Lactobacillus / Q000302:isolation & purification; D024821:Metabolic Syndrome / Q000628:therapy*; D008954:Models, Biological; D009844:Oligosaccharides / Q000032:analysis; D056692:Prebiotics / Q000008:administration & dosage*
chemical_list: D004337:Drug Carriers; D005232:Fatty Acids, Volatile; D009844:Oligosaccharides; D056692:Prebiotics
keywords: Prebiotic functionality; human intestinal microbiota; immune modulation