title: Elevated expression of TGIF is involved in lung carcinogenesis.
abstract: The purpose of this study was to explore the expression of TG-interacting factor (TGIF) in lung carcinogenesis. Malignant transformation of human bronchial epithelial (16HBE) cell was established by benzo(a)pyrene (BaP) treatment. Soft agar assay and tumor formation assay in nude mice were applied. Tumorigenesis experiment in vivo was done by BaP treatment. Western blotting, immunohistochemistry, and quantitative polymerase chain reaction were used to detect TGIF expression. We observed a higher level of TGIF messenger RNA (mRNA) in lung cancer tissues than that in paracancerous tissues. We observed significantly higher levels of TGIF mRNA and protein in A549 and H1299 cell lines than that in 16HBE cell. Increased expressions of TGIF protein and mRNA were observed in 16HBE cells induced by BaP treatment as compared to those in solvent control group. We observed significantly higher levels of TGIF mRNA and protein in 16HBE-BaP cells than that in 16HBE-control cells. We observed significantly higher levels of TGIF mRNA and protein in mice lung tissues treated with BaP than that in control group. Our results suggested that elevated expression of TGIF was involved in lung carcinogenesis.
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China. wangyd76@yahoo.com.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhai
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China.
authors.forename: Jiangmin
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China.
authors.forename: Congke
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001564:Benzo(a)pyrene / Q000633:toxicity; D001980:Bronchi / Q000187:drug effects / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D051379:Mice; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D018398:Homeodomain Proteins; D012097:Repressor Proteins; C097504:TGIF1 protein, human; D001564:Benzo(a)pyrene
keywords: Benzo(a)pyrene; Lung cancer; Lung carcinogenesis; TGIF