title: Determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans: An in vitro study.
abstract: BACKGROUND
  The aim of this study was to evaluate the antibacterial activity of pure green coffee bean extract on periodonto pathogenic bacteria Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa).
  
  
  METHODS
  Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBC) were used to assess the antibacterial effect of pure green coffee bean extract against periodonto pathogenic bacteria by micro dilution method and culture method, respectively.
  
  
  RESULTS
  MIC values of Pg, Pi and Aa were 0.2 μg/ml whereas Fn showed sensitive at concentration of 3.125 μg/ml. MBC values mirrors the values same as that of MIC.
  
  
  CONCLUSIONS
  Antimicrobial activity of pure green coffee bean extract against Pg, Pi, Fn and Aa suggests that it could be recommended as an adjunct to mechanical therapy in the management of periodontal disease.
authors.affiliation: Department of Periodontics, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
authors.forename: Nagaraj
authors.initials: N
authors.lastname: Bharath
authors.affiliation: Department of Periodontics, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
authors.forename: Nagur Karibasappa
authors.initials: NK
authors.lastname: Sowmya
authors.affiliation: Department of Periodontics, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
authors.forename: Dhoom Singh
authors.initials: DS
authors.lastname: Mehta
keywords: Antibacterial activity; green coffee bean extract; minimum bactericidal concentration; minimum inhibitory concentration; periodontogenic bacteria