title: [Experimental study on determination of viability of Schistosoma japonicum cercariae by staining].
abstract: OBJECTIVE
  To determine the viability of Schistosoma japonicum cercariae by staining.
  
  
  METHODS
  Schistosoma japonicum cercariae were stained by 0.4% trypan blue, 0.5% methylene blue-eosin-borax (M.E.B), 0.5% eosin, 0.5% methylene blue and 0.05% neutral red, respectively, for 5 min, then they were observed under a stereoscopic microscope.
  
  
  RESULTS
  The dead cercariae were stained in the trypan blue, M.E.B, eosin and neutral red, but unstained in the methylene blue. The vital cercariae were unstained in all the five kinds of dyes.
  
  
  CONCLUSIONS
  The staining methods by using 0.4% trypan blue, 0.5%M.E.B, 0.5% eosin and 0.05% neutral red can be used to determine the viability of S. japonicum cercariae.
authors.forename: Qin-lu
authors.initials: QL
authors.lastname: Bao
authors.forename: Chun-lan
authors.initials: CL
authors.lastname: Huang
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Chen
authors.forename: Xue-long
authors.initials: XL
authors.lastname: Wang
authors.forename: Shao-sheng
authors.initials: SS
authors.lastname: Wang
authors.forename: Xiao-niu
authors.initials: XN
authors.lastname: Tang
authors.forename: Shu-lin
authors.initials: SL
authors.lastname: Zhou
mesh_terms: D000818:Animals; D007814:Larva / Q000737:chemistry / Q000254:growth & development; D012549:Schistosoma japonicum / Q000737:chemistry / Q000254:growth & development*; D013194:Staining and Labeling / Q000379:methods*