title: Oxidative Decarboxylation of Short-Chain Fatty Acids to 1-Alkenes.
abstract: The enzymatic oxidative decarboxylation of linear short-chain fatty acids (C4:0-C9:0) employing the P450 monooxygenase OleT, O2 as the oxidant, and NAD(P)H as the electron donor gave the corresponding terminal C3 to C8  alkenes with product titers of up to 0.93 g L(-1) and TTNs of >2000. Key to this process was the construction of an efficient electron-transfer chain employing putidaredoxin CamAB in combination with NAD(P)H recycling at the expense of glucose, formate, or phosphite. This system allows for the biocatalytic production of industrially important 1-alkenes, such as propene and 1-octene, from renewable resources for the first time.
authors.affiliation: Department of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria).
authors.forename: Alexander
authors.initials: A
authors.lastname: Dennig
authors.affiliation: Department of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria).
authors.forename: Miriam
authors.initials: M
authors.lastname: Kuhn
authors.affiliation: Department of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria).
authors.forename: Sebastian
authors.initials: S
authors.lastname: Tassoti
authors.affiliation: Creavis, Evonik Industries, Bau 1420, Paul Baumann Strasse 1, 45772 Marl (Germany).
authors.forename: Anja
authors.initials: A
authors.lastname: Thiessenhusen
authors.affiliation: Creavis, Evonik Industries, Bau 1420, Paul Baumann Strasse 1, 45772 Marl (Germany).
authors.forename: Stefan
authors.initials: S
authors.lastname: Gilch
authors.affiliation: Creavis, Evonik Industries, Bau 1420, Paul Baumann Strasse 1, 45772 Marl (Germany).
authors.forename: Thomas
authors.initials: T
authors.lastname: Bülter
authors.affiliation: Creavis, Evonik Industries, Bau 1420, Paul Baumann Strasse 1, 45772 Marl (Germany).
authors.forename: Thomas
authors.initials: T
authors.lastname: Haas
authors.affiliation: Department of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria).
authors.forename: Mélanie
authors.initials: M
authors.lastname: Hall
authors.affiliation: Department of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria). Kurt.Faber@Uni-Graz.at.
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
mesh_terms: D000475:Alkenes / Q000378:metabolism*; D003653:Decarboxylation; D005227:Fatty Acids / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000475:Alkenes; D005227:Fatty Acids; D005288:Ferredoxins; D009243:NAD; C100137:putidaredoxin; D010105:Oxygenases; D010100:Oxygen
keywords: 1-alkenes; OleT; biocatalysis; fatty acids; oxidative decarboxylation