title: Potential mechanism for osseointegration of dental implants in Zucker diabetic fatty rats.
abstract: Our aim was to investigate the impact of diabetes mellitus and different durations of glycaemic control on early osseointegration of dental implants, and to explore possible mechanisms by measuring the expression of integrin α5β1 and fibronectin in bone around the implant. We divided 33 male Zucker diabetic fatty (ZDF) rats aged 3 months into 3 groups. The first group comprised diabetic rats with dental implants (controls); the second group was treated with insulin and implants were placed simultaneously (exenatide alone group); and the third group was treated with insulin until the serum glucose was at a constant concentration (< 16 mmol/L), and implants were then inserted (exenatide+normal glucose group). Rats were killed 7, 14, 30, and 60 days after implants had been inserted. The expression of integrin α5β1 and fibronectin in bone around the implants was detected by immunohistochemical analysis in each group. The expression in the exenatide+normal glucose group was stronger than in the other 2 groups. Fourteen days after implantation, expression of integrin α5β1 in the exenatide alone group was significantly stronger than that in the control group (p=0.027), and 60 days after implantation the expression of fibronectin in the exenatide alone group was also significantly stronger than that among the controls (p=0.001). Both fibronectin and integrin α5β1 participate in the adhesion of osteoblasts and act as signals at the bone/implant interface. Diabetes interferes with the osseointegration of implants by deferring expression of fibronectin and integrin α5β1.
authors.affiliation: Department of Implant Dentistry, School of Stomatology, Shandong University, No.44-1 West culture road, Lixia District, Jinan 250012, P.R. China; Department of Dental Implantology, Yantai Stomatological Hospital, No. 142, North Great Str., Zhifu District, Yantai 264008, P.R. China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital, No. 142, North Great Str., Zhifu District, Yantai 264008, P.R. China; Orthodontics & Periodontology Research Unit, Bernhard-Gottlieb-University Clinic of Dentistry, Sensengasse 2a-1090 Vienna, Austria.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Sensengasse 2a-1090 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Tangl
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital, No. 142, North Great Str., Zhifu District, Yantai 264008, P.R. China.
authors.forename: Shutai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Implant Dentistry, School of Stomatology, Shandong University, No.44-1 West culture road, Lixia District, Jinan 250012, P.R. China. Electronic address: 15953505985@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital, No. 142, North Great Str., Zhifu District, Yantai 264008, P.R. China; Orthodontics & Periodontology Research Unit, Bernhard-Gottlieb-University Clinic of Dentistry, Sensengasse 2a-1090 Vienna, Austria.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Rausch-Fan
mesh_terms: D000818:Animals; D003757:Dental Implantation; D015921:Dental Implants / Q000706:statistics & numerical data*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000601:surgery*; D000077270:Exenatide; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000627:therapeutic use; D039081:Integrin alpha5beta1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D016348:Osseointegration; D010455:Peptides / Q000627:therapeutic use; D051381:Rats; D011924:Rats, Zucker; D016896:Treatment Outcome; D014688:Venoms / Q000627:therapeutic use
chemical_list: D015921:Dental Implants; D005353:Fibronectins; D007328:Insulin; D039081:Integrin alpha5beta1; D010455:Peptides; D014688:Venoms; D000077270:Exenatide
keywords: Dental implant; Diabetes mellitus; Fibronectin; Glycemic Control; Hyperglycemia; Integrinα5β1; Osseointegration