title: [Diagnosis value of combined detection of serum golgi protein 73, desgamma carboxy prothrombin and α-fetoprotein in primary hepatic carcinoma].
abstract: OBJECTIVE
  To analyze the clinical value of the detection of serum desgamma carboxy prothrombin (DCP), golgi glycoprotein 73 (GP73), heat-shock protein 70 (HSP70) in primary hepatic carcinoma (PHC) diagnosis.
  
  
  METHODS
  Enzyme-1inked immunosorbent assay and electrochemiluminescence immunoassay were used to detect the serum DCP, GP73, HSP70 and α-fetoprotein (AFP) levels in 35 PHC patients and 35 healthy controls.
  
  
  RESULTS
  AFP, DCP and GP73 levels in PHC patients were 13.780 (1.140-8 487.000)µg/L, 3 213.953 (2.510-53 994.602)pg/ml and 76.838 (24.500-232.875)ng/ml respectively, significantly higher than those in healthy controls (1.240 (0.605-5.310)µg/L, 104.610 (0.000-4 138.770)pg/ml and 30.770 (16.343-87.453)ng/ml, U value were 134.50, 258.00 and 168.00, all P < 0.01); HSP70 could not be detected in any objects. The area under the ROC curve of DCP or GP73 was 0.789, 0.863 respectively. The sensitivity and specificity to PHC diagnosis were 54.3% and 97.1% by DCP (2 939.4 pg/ml as cut-off value), 85.7% and 74.3% by GP73 (41.3 ng/ml as cut-off value), 45.7% and 100% by AFP (20 µg/L as cut-off value). In combined detection the sensitivity, specificity and accuracy were 88.6%, 74.3%, 81.4% by GP73/AFP and 91.4%, 71.4%, 81.4% by GP73/DCP/AFP.
  
  
  CONCLUSIONS
  GP73, DCP are new effective markers in the diagnosis of PHC, and the combined detection of GP73, DCP and AFP can improve the diagnosis value of PHC.
authors.affiliation: Department of Biochemistry, Guilin Medical University, Guilin 541004, China; Email: huo_qun@sina.com.
authors.forename: Qun
authors.initials: Q
authors.lastname: Huo
authors.forename: Zehui
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Chan
authors.initials: C
authors.lastname: Li
authors.forename: Chunping
authors.initials: C
authors.lastname: Hu
mesh_terms: D015415:Biomarkers; D006528:Carcinoma, Hepatocellular; D018840:HSP70 Heat-Shock Proteins; D006490:Hemostatics; D006801:Humans; D008113:Liver Neoplasms; D011516:Prothrombin; D012372:ROC Curve; D000509:alpha-Fetoproteins
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D006490:Hemostatics; D000509:alpha-Fetoproteins; D011516:Prothrombin