title: Assembly of Multigene Constructs Using Golden Gate Cloning.
abstract: Efficient DNA assembly methods are required for synthetic biology. Standardization of DNA parts is an essential element that not only facilitates reuse of the same parts for various constructs but also allows standardization of the assembly strategy. We provide here a protocol for assembly of multigene constructs from standard biological parts using the modular cloning system MoClo. Making constructs using this system requires to first define the structure of the final construct and to identify all basic parts and vectors required for the construction strategy. The cloning strategy is in large part determined by the structure of the final construct, which is then made using a series of one-pot Golden Gate cloning reactions.
authors.affiliation: Department of Cell and Metabolic Biology, Leibniz-Institut für Pflanzenbiochemie, Halle, Germany.
authors.forename: Sylvestre
authors.initials: S
authors.lastname: Marillonnet
authors.forename: Stefan
authors.initials: S
authors.lastname: Werner
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D005810:Multigene Family / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods
chemical_list: D018744:DNA, Plant