title: C-reactive protein and high mobility group box 1 in dogs with gastric dilatation and volvulus.
abstract: OBJECTIVE
  To (1) measure C-reactive protein (CRP) and high mobility group box 1 (HMGB1) and (2) evaluate their prognostic value and relationship to severity of systemic inflammatory response syndrome, routine hematological and acid-base parameters in dogs with gastric dilatation volvulus (GDV).
  
  
  METHODS
  Prospective observational study from September 2010 to June 2012.
  
  
  METHODS
  Veterinary teaching hospital.
  
  
  METHODS
  Forty-one client-owned dogs with GDV.
  
  
  METHODS
  None.
  
  
  RESULTS
  Blood was collected before surgery (baseline), postsurgery, 6-10 hours postsurgery, and 18-22 hours postsurgery. CRP and HMGB1 were measured in all samples, and routine hematological, biochemical, and acid-base analyses were performed. Only baseline and postsurgery samples were used from nonsurvivors (n = 10). CRP increased significantly from postsurgery sampling to 18-22 hours postsurgery, while HMGB1 did not change over time. There was a significant difference in HMGB1 between survivors and nonsurvivors over time. Both proteins correlated with systemic inflammatory response syndrome severity, total leukocyte, segmented neutrophils, and band counts. HMGB1 correlated also with acid-base parameters (pH, bicarbonate, base excess).
  
  
  CONCLUSIONS
  HMGB1 and CRP behaved differently in regards to their kinetic patterns, with HMGB1 appearing to better reflect the severity of tissue injury in dogs with GDV than CRP.
authors.affiliation: Department of Physiology, Brno, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Uhrikova
authors.affiliation: Small Animal Clinic, Department of Surgery and Orthopedics, Brno, Czech Republic.
authors.forename: Leona
authors.initials: L
authors.lastname: Rauserova-Lexmaulova
authors.affiliation: Small Animal Clinical Laboratory, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Kristina
authors.initials: K
authors.lastname: Rehakova
authors.affiliation: Department of Physiology, Brno, Czech Republic.|Integrated Center of Cellular Therapy (ICCT), St. Annes´s University Hospital - International Clinical Research Center Brno, Brno, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Scheer
authors.affiliation: Department of Physiology, Brno, Czech Republic.|Small Animal Clinical Laboratory, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doubek
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000378:metabolism*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D004630:Emergencies / Q000662:veterinary; D005260:Female; D013271:Gastric Dilatation / Q000601:surgery / Q000662:veterinary; D024243:HMGB1 Protein / Q000097:blood*; D008297:Male; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D013277:Stomach Volvulus / Q000601:surgery / Q000662:veterinary; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D015415:Biomarkers; D024243:HMGB1 Protein; D002097:C-Reactive Protein
keywords: canine; mortality; prognosis; systemic inflammatory response syndrome