title: Chokeberry (Aronia melanocarpa (Michx.) Elliot) concentrate inhibits NF-κB and synergizes with selenium to inhibit the release of pro-inflammatory mediators in macrophages.
abstract: Black chokeberry has been known to play a protective role in human health due to its high polyphenolic content including anthocyanins and caffeic acid derivatives. In the present study, we first characterized the polyphenolic content of a commercial chokeberry concentrate and investigated its effect on LPS-induced NF-κB activation and release of pro-inflammatory mediators in macrophages in the presence or the absence of sodium selenite. Examination of the phytochemical profile of the juice concentrate revealed high content of polyphenols (3.3%), including anthocyanins, proanthocyanidins, phenolic acids, and flavonoids. Among them, cyanidin-3-O-galactoside and caffeoylquinic acids were identified as the major compounds. Data indicated that chokeberry concentrate inhibited both the release of TNFα, IL-6 and IL-8 in human peripheral monocytes and the activation of the NF-κB pathway in RAW 264.7 macrophage cells. Furthermore, chokeberry synergizes with sodium selenite to inhibit NF-κB activation, cytokine release and PGE2 synthesis. These findings suggest that selenium added to chokeberry juice enhances significantly its anti-inflammatory activity, thus revealing a sound approach in order to tune the use of traditional herbals by combining them with micronutrients.
authors.affiliation: VivaCell Biotechnology GmbH, Denzlingen, Germany.
authors.forename: Kurt
authors.initials: K
authors.lastname: Appel
authors.affiliation: Ursapharm Arzneimittel GmbH, Saarbrücken, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Meiser
authors.affiliation: IMIBIC, Reina Sofía University Hospital, University of Córdoba, Dept of Cell Biology, Physiology and Immunology, Avda Menéndez Pidal s/n 14004, Córdoba, Spain.
authors.forename: Estrella
authors.initials: E
authors.lastname: Millán
authors.affiliation: IMIBIC, Reina Sofía University Hospital, University of Córdoba, Dept of Cell Biology, Physiology and Immunology, Avda Menéndez Pidal s/n 14004, Córdoba, Spain.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Collado
authors.affiliation: VivaCell Biotechnology GmbH, Denzlingen, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Rose
authors.affiliation: Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Gras
authors.affiliation: Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany; Biological Science Department, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Reinhold
authors.initials: R
authors.lastname: Carle
authors.affiliation: IMIBIC, Reina Sofía University Hospital, University of Córdoba, Dept of Cell Biology, Physiology and Immunology, Avda Menéndez Pidal s/n 14004, Córdoba, Spain. Electronic address: e.munoz@uco.es.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D004357:Drug Synergism; D000067030:Fruit and Vegetable Juices; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D046936:Photinia / Q000737:chemistry*; D064209:Phytochemicals / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D012643:Selenium / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D018038:Sodium Selenite / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C508606:CXCL8 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D064209:Phytochemicals; D059808:Polyphenols; D014409:Tumor Necrosis Factor-alpha; D012643:Selenium; D018038:Sodium Selenite; D015232:Dinoprostone
keywords: Aronia melanocarpa; Cytokines; NF-κB; Prostaglandin E(2); Selenium