title: Low expression of activation marker CD69 and chemokine receptors CCR5 and CXCR3 on memory T cells after 2009 H1N1 influenza A antigen stimulation in vitro following H1N1 vaccination of HIV-infected individuals.
abstract: Unlike well-studied antibody responses to pandemic 2009 H1N1 influenza A virus vaccines in human immunodeficiency virus-infected (HIV+) individuals, less well understood are cell-mediated immune (CMI) responses to this antigen in this susceptible population. We investigated such influenza-specific CMI responses in 61 HIV+ individuals and in 20 HIV-negative (HIV-) healthy controls. Each was vaccinated with a single licensed dose of inactivated, split-virion vaccine comprised of the influenza A/California/7/2009 (H1N1) virus-like strain. Cells collected just prior to vaccination and at 1 and 3 months afterwards were stimulated in vitro with dialyzed vaccine antigen and assayed by flow cytometry for cytokines TNF-α, IFN-γ, IL-2, and IL-10, for degranulation marker CD107a, as well as phenotypes of memory T-cell subpopulations. Comparable increases of cytokine-producing and CD107a-expressing T cells were observed in both HIV+ subjects and healthy HIV-controls. However, by 3 months post-vaccination, in vitro antigen stimulation of peripheral blood mononuclear cells induced greater expansion in controls of both CD4 and CD8 central memory and effector memory T cells, as well as higher expression of the activation marker CD69 and chemokine receptors CCR5 and CXCR3 than in HIV+ subjects. We concluded CD4+ and CD8+ memory T cells produce cytokines at comparable levels in both groups, whereas the expression after in vitro stimulation of molecules critical for cell migration to infection sites are lower in the HIV+ than in comparable controls. Further immunization strategies against influenza are needed to improve the CMI responses in people living with HIV.
authors.affiliation: a Faculty of Associated Medical Sciences ; Chiang Mai University ; Chiang Mai , Thailand.
authors.forename: Kriangkrai
authors.initials: K
authors.lastname: Chawansuntati
authors.forename: Nuntisa
authors.initials: N
authors.lastname: Chotirosniramit
authors.forename: Patcharaphan
authors.initials: P
authors.lastname: Sugandhavesa
authors.forename: Linda
authors.initials: L
authors.lastname: Aurpibul
authors.forename: Sunida
authors.initials: S
authors.lastname: Thetket
authors.forename: Natthapol
authors.initials: N
authors.lastname: Kosashunhanan
authors.forename: Taweewat
authors.initials: T
authors.lastname: Supindham
authors.forename: Oranitcha
authors.initials: O
authors.lastname: Kaewthip
authors.forename: Piyathida
authors.initials: P
authors.lastname: Sroysuwan
authors.forename: Thira
authors.initials: T
authors.lastname: Sirisanthana
authors.forename: Khuanchai
authors.initials: K
authors.lastname: Suparatpinyo
authors.forename: Jiraprapa
authors.initials: J
authors.lastname: Wipasa
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control*; D037181:Lectins, C-Type / Q000032:analysis; D008297:Male; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000032:analysis; D054367:Receptors, CXCR3 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D055815:Young Adult; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016207:Cytokines; D007252:Influenza Vaccines; D037181:Lectins, C-Type; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D000099192:CD69 Antigens; C584933:CCR5 protein, human; C515187:CXCR3 protein, human
keywords: 2009 H1N1 influenza A vaccine; HIV-infection; activation markers; cellular immunity; chemokine receptors; immunological memory responses