title: Combining Passive Sampling with Recombinant Receptor-Reporter Gene Bioassays to Assess the Receptor Activity of Victorian Rivers.
abstract: This pilot study was initiated to provide new information on the 'hormonal' activity of Victorian rivers. Chemcatcher™ passive sampler systems containing Empore™ C18FF disks were deployed at eight riverine sites near Melbourne. Little estrogenic activity [<0.4-1.8 ng estradiol equivalents (EQ)/disk] and no retinoic acid activity (RAR, all samples <0.8 ng trans-retinoic acid EQ/disk) was observed. Almost all sample extracts showed aryl hydrocarbon receptor activity (from <4 to 29 ng β-naphthoflavone EQ/disk). Overall, the disk extracts were eminently compatible with the bioassay screening technology, enabling the relative levels of 'hormonal activity' to be observed in the surface waters in and around Melbourne. From a practical perspective, the in situ sampling and pre-concentration provided by passive sampling reduces the manual handling risks associated with sample transport, and the number of laboratory operations required to obtain assay-ready solutions for analysis.
authors.affiliation: School of Applied Sciences, RMIT University, Melbourne, VIC, 3001, Australia. graeme.allinson@rmit.edu.au.|Future Farming Systems Research, Department of Primary Industries, DPI Queenscliff Centre, Queenscliff, VIC, 3225, Australia. graeme.allinson@rmit.edu.au.
authors.forename: Graeme
authors.initials: G
authors.lastname: Allinson
authors.affiliation: Center for Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.
authors.forename: Fujio
authors.initials: F
authors.lastname: Shiraishi
authors.affiliation: Laboratory of Toxicology, School of Veterinary Medicine, Kitasato University, 35-1 Higashi 23-bancho, Towada-Shi, Aomori, 034-8628, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kamata
authors.affiliation: Future Farming Systems Research, Department of Primary Industries, DPI Queenscliff Centre, Queenscliff, VIC, 3225, Australia.|Centre for Aquatic Pollution Identification and Management (CAPIM), School of Chemistry, The University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Allinson
mesh_terms: D000818:Animals; D001681:Biological Assay; D004784:Environmental Monitoring / Q000379:methods*; D017930:Genes, Reporter; D006801:Humans; D009990:Oryzias / Q000378:metabolism; D010865:Pilot Projects; D018336:Receptors, Aryl Hydrocarbon / Q000737:chemistry; D011960:Receptors, Estrogen / Q000737:chemistry; D018168:Receptors, Retinoic Acid / Q000737:chemistry; D045483:Rivers / Q000737:chemistry*; D014739:Victoria; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D060753:Water Quality; D015003:Yeasts / Q000378:metabolism
chemical_list: D018336:Receptors, Aryl Hydrocarbon; D011960:Receptors, Estrogen; D018168:Receptors, Retinoic Acid; D014874:Water Pollutants, Chemical
keywords: Aryl hydrocarbon receptor activity; Australia; Chemcatcher passive sampler; Estrogenic activity; Yarra River; Yeast-based two-hybrid recombinant receptor–reporter gene assay activity