title: Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.
abstract: In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.
authors.affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.
authors.forename: Yuanda
authors.initials: Y
authors.lastname: Du
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, 250014, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Feng
authors.affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China; School of Life Sciences, Shandong University, Jinan, 250100, China; Shandong Provincial Engineering and Technology Research Center for Vegetation Ecology, Shandong University, Jinan, 250100, China.
authors.forename: Renqing
authors.initials: R
authors.lastname: Wang
authors.affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.
authors.forename: Haijie
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D047311:Luteolin / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D031821:Potamogetonaceae / Q000737:chemistry*; D012333:RNA, Messenger / Q000187:drug effects
chemical_list: D005419:Flavonoids; D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D047311:Luteolin