title: Effects of ageing on metabolite and oxidant concentrations in different regions of rat kidney under normal and stress conditions.
abstract: Metabolic and oxidative stresses have been implicated in ageing and the pathogenesis of chronic kidney disease. In this study, we investigated the glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and lactate concentrations in different kidney regions under control conditions and after exposure to oxidative stress invoked by 0.2 mM H2O2. Slices of superficial cortex, outer or inner medulla were dissected from kidneys of male Wistar rats of 5-, 12-, 36- and 60-week old. Samples were incubated for 30 min ± 0.2 mM H2O2 prior to homogenisation and centrifugation. The concentrations of GSH, TBARS and lactate were measured by colorimetry. Each metabolite showed a distinctive pattern. For GSH, this was 12 weeks > 36 weeks > 60 weeks and 5 weeks with the highest concentration measured in the superficial cortex at 12 weeks. For TBARS and lactate, the pattern was for the lowest concentration at 12 weeks and the highest at 60 and 5 weeks. The highest lactate and TBARS concentrations were measured under oxidative stress conditions, particularly at 5 and 60 weeks. These results suggest that GSH in different kidney regions peaks at maturity and then reduces with increasing age.
authors.affiliation: School of Science and Technology, Centre for Bioactive Discovery in Health and Ageing, University of New England, Armidale, NSW, 2351, Australia.
authors.forename: Noor Riyadh
authors.initials: NR
authors.lastname: Thiab
authors.affiliation: School of Biomedical and Healthcare Sciences, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth University, Plymouth, PL4 8AA, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: King
authors.affiliation: School of Science and Technology, Centre for Bioactive Discovery in Health and Ageing, University of New England, Armidale, NSW, 2351, Australia. gjones2@une.edu.au.
authors.forename: Graham L
authors.identifier: http://orcid.org/0000-0002-6435-1542
authors.initials: GL
authors.lastname: Jones
mesh_terms: D000367:Age Factors; D000818:Animals; D005978:Glutathione / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007668:Kidney / Q000737:chemistry* / Q000502:physiology; D019344:Lactic Acid / Q000032:analysis*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D019344:Lactic Acid; D006861:Hydrogen Peroxide; D005978:Glutathione
keywords: Ageing; Kidney region; Oxidative stress and lactate