title: Condurango 30C Induces Epigenetic Modification of Lung Cancer-specific Tumour Suppressor Genes via Demethylation.
abstract: BACKGROUND
  DNA hypermethylation induces cancer progression involving CpG island of DNA and causes inactivation of tumour suppressor genes. In this study, DNA hypermethylation status of lung cancer and ability of ultra-highly diluted Condurango 30C to modulate DNA methylation were ascertained by analysis of lung cancer-specific tumour suppressor genes in respect to placebo.
  
  
  METHODS
  DNA methylation status, if any, was determined by PCR-SSCP analyses in lung cancer-specific tumour suppressor genes (p15, p16 and p53) using H460-NSCLC cell and BaP-induced lung cancer of rats. The ability of Condurango 30C to modulate DNA methylation, if any, was verified against placebo control in blinded manner.
  
  
  RESULTS
  Condurango 30C-treated DNA showed significant decrease in band intensity of p15 and p53 genes especially in methylated condition in vitro, at IC50 dose (2.43µl/100µl). SSCP analysis of p15 and p53 genes in Condurango 30C-treated DNA also suggests that Condurango 30C can decrease methylation, in vitro. Inhibition of p15 hypermethylation was observed in post-cancer treatment of rats with Condurango 30C. SSCP results gave a better indication of differences in band position of p15 and p53 in Condurango 30C-treated lung samples.
  
  
  CONCLUSIONS
  Condurango 30C could trigger epigenetic modification in lung cancer via modulation of DNA hypermethylation.
authors.affiliation: Department of Zoology, University of Kalyani, Kalyani, India.
authors.forename: Anisur R
authors.initials: AR
authors.lastname: Khuda-Bukhsh
authors.forename: Sourav
authors.initials: S
authors.lastname: Sikdar
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects; D019175:DNA Methylation / Q000187:drug effects*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D016147:Genes, Tumor Suppressor; D005956:Glucose-6-Phosphate Isomerase; D008168:Lung / Q000187:drug effects; D031101:Marsdenia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D005956:Glucose-6-Phosphate Isomerase