title: In Vivo Antioxidant and Hypolipidemic Effects of Fermented Mung Bean on Hypercholesterolemic Mice.
abstract: Legumes have previously been reported with hypolipidemic effect caused by the presence of flavonoid. This study was carried out to evaluate the antioxidant and hypolipidemic effects of fermented mung bean on hypercholesterolemic mice. Blood from all mice was collected and subjected to serum lipid and liver profiles biochemical analysis and quantitative RT-PCR for atherosclerosis related gene expressions. Besides, livers were collected for antioxidant assays and histopathology evaluation. Fermented mung bean was found to reduce the level of serum lipid and liver enzyme profiles of hypercholesterolemic mice. Furthermore, liver antioxidant and nitric oxide levels were also significantly restored by fermented mung bean in a dosage dependent manner. The gene expression study indicated that Apoe and Bcl2a1a were upregulated while Npy and Vwf expressions were downregulated after the treatment. The effects of fermented mung bean were greater than nonfermented mung bean. These results indicated that fermented mung bean possessed antioxidants that lead to its hypolipidemic effect on hypercholesterolemic mice.
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Swee Keong
authors.identifier: 0000-0002-4736-0674
authors.initials: SK
authors.lastname: Yeap
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Boon Kee
authors.initials: BK
authors.lastname: Beh
authors.affiliation: School of Biomedical Sciences, The University of Nottingham, Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia.
authors.forename: Wan Yong
authors.initials: WY
authors.lastname: Ho
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Hamidah
authors.initials: H
authors.lastname: Mohd Yusof
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Nurul Elyani
authors.identifier: 0000-0003-3461-8602
authors.initials: NE
authors.lastname: Mohamad
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Norlaily Mohd
authors.initials: NM
authors.lastname: Ali
authors.affiliation: Biotechnology Research Centre, Malaysian Agriculture Research Development Institute, 43400 Serdang, Selangor, Malaysia.
authors.forename: Indu Bala
authors.initials: IB
authors.lastname: Jaganath
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
authors.affiliation: Department of Bioprocess Biotechnology, Malaysian Agriculture Research Development Institute, 43400 Serdang, Selangor, Malaysia.
authors.forename: Soo Peng
authors.initials: SP
authors.lastname: Koh
authors.affiliation: Department of Bioprocess Biotechnology, Malaysian Agriculture Research Development Institute, 43400 Serdang, Selangor, Malaysia.
authors.forename: Kamariah
authors.identifier: 0000-0002-3785-2106
authors.initials: K
authors.lastname: Long