title: Steady state preparative multiple dual mode counter-current chromatography: Productivity and selectivity. Theory and experimental verification.
abstract: In the steady state (SS) multiple dual mode (MDM) counter-current chromatography (CCC), at the beginning of the first step of every cycle the sample dissolved in one of the phases is continuously fed into a CCC device over a constant time, not exceeding the run time of the first step. After a certain number of cycles, the steady state regime is achieved, where concentrations vary over time during each cycle, however, the concentration profiles of solutes eluted with both phases remain constant in all subsequent cycles. The objective of this work was to develop analytical expressions to describe the SS MDM CCC separation processes, which can be helpful to simulate and design these processes and select a suitable compromise between the productivity and the selectivity in the preparative and production CCC separations. Experiments carried out using model mixtures of compounds from the GUESSmix with solvent system hexane/ethyl acetate/methanol/water demonstrated a reasonable agreement between the predictions of the theory and the experimental results.
authors.affiliation: Kurnakov Institute of General & Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, Moscow 119991, Russia. Electronic address: kost@igic.ras.ru.
authors.forename: Artak E
authors.initials: AE
authors.lastname: Kostanyan
authors.affiliation: Kurnakov Institute of General & Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, Moscow 119991, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Erastov
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003377:Countercurrent Distribution; D006586:Hexanes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D006586:Hexanes; D012997:Solvents; D014867:Water; C026385:n-hexane; D000432:Methanol
keywords: Multiple dual mode counter-current chromatography; Peak equations; Periodic sample loading; Steady state operation