title: Molecular characterization of the Tobacco rattle virus RNA2 genome isolated from Gladiolus.
abstract: Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3' terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.
authors.affiliation: Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University , Seoul 139-774 , Korea.
authors.forename: Yoon J
authors.initials: YJ
authors.lastname: Kim
authors.forename: Mi S
authors.initials: MS
authors.lastname: Lim
authors.forename: Su M
authors.initials: SM
authors.lastname: Kim
authors.forename: Ki H
authors.initials: KH
authors.lastname: Ryu
authors.forename: Sun H
authors.initials: SH
authors.lastname: Choi
mesh_terms: D016679:Genome, Viral; D019684:Magnoliopsida / Q000821:virology*; D010802:Phylogeny; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012367:RNA, Viral
keywords: Gladiolus; Korean isolate; RNA2; Tobacco rattle virus; phylogeny