title: [Renal sympathetic denervation suppresses ventricular substrate remodeling in a canine high-rate pacing model].
abstract: OBJECTIVE
  To explore the role of renal sympathetic denervation (RSD) on ventricular substrate remodeling in dogs with pacing-induced heart failure (HF).
  
  
  METHODS
  A total of 19 dogs were randomized into 3 groups of sham-operated control (n = 7), right ventricular pacing induction of HF (n = 6) and RSD (n = 6). After 8-week pacing induction of HF. Hemodynamic variables were monitored at baseline and after HF. Masson's trichrome staining, enzyme-linked immunosorbent assay (ELISA) and Western blot were used to measure ventricular interstitial fibrosis, brain natriuretic peptide (BNP), angiotensin II (Ang II), aldosterone and transforming growth factor-beta (TGF-β).
  
  
  RESULTS
  All dogs in HF and HF+RSD groups showed increased left and right ventricular diastolic dimensions [left ventricle: (27.0 ± 2.4) vs (37.0 ± 2.8) mm, P < 0.01 and (30.0 ± 2.5) vs (36.0 ± 2.8) mm, P < 0.05; right ventricle: (11.0 ± 1.5) vs (14.0 ± 1.7) mm, P = 0.03 and (12.0 ± 1.1) vs (14.0 ± 1.2) mm, P < 0.05]. Compared with HF + RSD dogs, HF dogs had higher left ventricular end-diastolic pressure (LVEDP) [(25.0 ± 3.7) vs (3.3 ± 1.6) mmHg, P < 0.01] and more fibrous tissue (left ventricle:24.1% ± 4.8% vs 8.5% ± 1.9%, P < 0.01; right ventricle:17.2% ± 5.2% vs 11.8% ± 3.9%, P < 0.01). The levels of BNP, Ang II, aldosterone and TGF-β in ventricular tissue increased in HF dogs compared to sham-operated and HF+RSD dogs.
  
  
  CONCLUSIONS
  RSD could suppress ventricular substrate remodeling induced by long-term rapid ventricular pacing.
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Xiaozhan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China; Email: ruyan1971@yahoo.com.cn.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Shengbo
authors.initials: S
authors.lastname: Yu
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Xule
authors.initials: X
authors.lastname: Wang
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Congxin
authors.initials: C
authors.lastname: Huang
mesh_terms: D000450:Aldosterone; D000804:Angiotensin II; D000818:Animals; D002304:Cardiac Pacing, Artificial; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006333:Heart Failure; D006352:Heart Ventricles; D006439:Hemodynamics; D023421:Models, Animal; D020097:Natriuretic Peptide, Brain; D013562:Sympathectomy; D020257:Ventricular Remodeling
chemical_list: D000804:Angiotensin II; D020097:Natriuretic Peptide, Brain; D000450:Aldosterone