title: Application of atomic force microscopy for investigation of Na(+),K(+)-ATPase signal-transducing function.
abstract: The Young's modulus of 10-12-day-old chick embryos' sensory neurons cultivated in dissociated cell culture was measured using a PeakForce Quantitative Nanomechanical Mapping atomic force microscopy. The native cells were tested in control experiments and after application of ouabain. At low "endogenous" concentration of 10⁻¹⁰ M, ouabain tended to increase the rigidity of sensory neurons. We hypothesize that this trend resulted from activation of Na⁺,K⁺-ATPase signal-transducing function.
authors.affiliation: Pavlov Institute of Physiology Russian Academy of Sciences , St. Petersburg , Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Khalisov
authors.forename: A V
authors.initials: AV
authors.lastname: Ankudinov
authors.forename: V A
authors.initials: VA
authors.lastname: Penniyaynen
authors.forename: D
authors.initials: D
authors.lastname: Dobrota
authors.forename: Boris V
authors.initials: BV
authors.lastname: Krylov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D055119:Elastic Modulus; D004789:Enzyme Activation; D020536:Enzyme Activators / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D018625:Microscopy, Atomic Force; D010042:Ouabain / Q000494:pharmacology; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000201:enzymology*; D015398:Signal Transduction / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Na+,K+-ATPase signal-transducing function; PeakForce QNM; atomic force microscopy; ouabain; sensory neurons