title: In vitro studies of phenethyl isothiocyanate against the growth of LN229 human glioma cells.
abstract: Phenethyl isothiocyanate (PEITC) is one of the best studied members of isothiocyanates (ITC), a variety of edible cruciferous vegetables including broccoli, watercress, and cabbage, and have generated particular interest because of its remarkable chemopreventive activity. Many literature reports proved that phenethyl isothiocyanate exhibited significant anti-cancer chemopreventive effects including lung, glioma and leukemia cancer. In this study, we explored the inhibitory effects as well as mechanisms of PEITC on human glioma LN229 cells. Results demonstrated that PEITC possesses the potential ability to inhibit proliferation, induce apoptosis and arrest cell cycling against LN229 human glioma cells. Moreover, investigated results showed that PEITC inhibited the expression of superoxide dismutase (SOD) and glutathione (GSH), and caused oxidative stress to tumor cells. Collective results suggested us to believe that PEITC can inhibit the growth of LN229 cells and its mechanism can be related to the fact that PEITC can cause oxidative stress to tumor cells.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Qingdao Branch, Shandong University Qingdao, Shandong Province, China ; Department of Neurosurgery, Taian Central Hospital Taian, Shandong Province, China.
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Su
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University Liaocheng, Shandong Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lin
authors.affiliation: Department of Emergency, Taishan Coal Sanatorium of Shandong Province Taian, Shandong Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University Liaocheng, Shandong Province, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Sui
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University Liaocheng, Shandong Province, China.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Qingdao Branch, Shandong University Qingdao, Shandong Province, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017879:Isothiocyanates; D017382:Reactive Oxygen Species; C058305:phenethyl isothiocyanate; D013482:Superoxide Dismutase; C505407:CASP3 protein, human; D053148:Caspase 3; D005978:Glutathione
keywords: LN229 cells; Phenethyl isothiocyanate; glioma