title: CD24 tracks divergent pluripotent states in mouse and human cells.
abstract: Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: Nika
authors.initials: N
authors.lastname: Shakiba
authors.affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.|The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: Carl A
authors.initials: CA
authors.lastname: White
authors.affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: Yonatan Y
authors.identifier: http://orcid.org/0000-0002-6022-8846
authors.initials: YY
authors.lastname: Lipsitz
authors.affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.|The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: Ayako
authors.initials: A
authors.lastname: Yachie-Kinoshita
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Tonge
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
authors.forename: Samer M I
authors.initials: SMI
authors.lastname: Hussein
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.|Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5T 3H7.
authors.forename: Mira C
authors.initials: MC
authors.lastname: Puri
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
authors.forename: Judith
authors.initials: J
authors.lastname: Elbaz
authors.affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: James
authors.initials: J
authors.lastname: Morrissey-Scoot
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
authors.forename: Mira
authors.initials: M
authors.lastname: Li
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.|Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Javier
authors.initials: J
authors.lastname: Munoz
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.|Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Marco
authors.initials: M
authors.lastname: Benevento
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.|Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.|Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada M5G 1E2.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Rogers
authors.affiliation: The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel 76100.
authors.forename: Jacob H
authors.identifier: http://orcid.org/0000-0003-2042-9974
authors.initials: JH
authors.lastname: Hanna
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.|Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Albert J R
authors.initials: AJR
authors.lastname: Heck
authors.affiliation: Department of Biology, Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zürich, Wolfgang-Pauli Strasse 16, 8093 Zürich, Switzerland.|NCCR Neuro Center for Proteomics, University and Swiss Federal Institute of Technology (ETH) Zürich, Wolfgang-Pauli Strasse 16, 8093 Zurich, Switzerland.|Department of Health Sciences and Technology, Swiss Federal Institute of Technology (ETH) Zürich, Universitätstrasse 2, 8092 Zurich, Switzerland.
authors.forename: Bernd
authors.identifier: http://orcid.org/0000-0002-3923-1610
authors.initials: B
authors.lastname: Wollscheid
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.|Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada M5G 1E2.|Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada M5T 3H7.
authors.forename: Andras
authors.initials: A
authors.lastname: Nagy
authors.affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.|The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Zandstra
mesh_terms: D000818:Animals; D051927:CD24 Antigen / Q000378:metabolism*; D065150:Cellular Reprogramming; D005855:Germ Layers / Q000166:cytology; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D051927:CD24 Antigen; C497889:CD24 protein, human; C497890:Cd24a protein, mouse