title: Derivation of a Homozygous Human Androgenetic Embryonic Stem Cell Line.
abstract: Human embryonic stem cells (hESCs) have long been considered as a promising source for cell replacement therapy. However, one major obstacle for the use of these cells is immune compatibility. Histocompatible human parthenogenetic ESCs have been reported as a new method for generating human leukocyte antigen (HLA)-matched hESCs. To further investigate the possibility of obtaining histocompatible stem cells from uniparental embryos, we tried to produce androgenetic haploid human embryos by injecting a single spermatozoon into enucleated human oocyte, and establish human androgenetic embryonic stem (hAGES) cell lines from androgenetic embryos. In the present study, a diploid hAGES cell line has been established, which exhibits typical features of human ESCs, including the expression of pluripotency markers, having differentiation potential in vitro and in vivo, and stable propagation in an undifferentiated state (>P40). Bisulfite sequencing of the H19, Snrpn, Meg3, and Kv imprinting control regions suggested that hAGES cells maintained to a certain extent a sperm methylation pattern. Genome-wide single nucleotide polymorphism, short tandem repeat, and HLA analyses revealed that the hAGES cell genome was highly homozygous. These results suggest that hAGES cells from spermatozoon could serve as a useful tool for studying the mechanisms underlying genomic imprinting in humans. It might also be used as a potential resource for cell replacement therapy as parthenogenetic stem cells.
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Chenhui
authors.initials: C
authors.lastname: Ding
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Sunxing
authors.initials: S
authors.lastname: Huang
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Quan
authors.initials: Q
authors.lastname: Qi
authors.affiliation: 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .
authors.forename: Rui
authors.initials: R
authors.lastname: Fu
authors.affiliation: 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .
authors.forename: Wanwan
authors.initials: W
authors.lastname: Zhu
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Pingping
authors.initials: P
authors.lastname: Hong
authors.affiliation: 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .
authors.forename: Zhengxin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .
authors.forename: Tiantian
authors.initials: T
authors.lastname: Gu
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: 3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: 1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .|2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000728:Androgens / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D004171:Diploidy; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006238:Haploidy; D018398:Homeodomain Proteins / Q000235:genetics; D006720:Homozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000235:genetics; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D055517:snRNP Core Proteins / Q000235:genetics
chemical_list: D000728:Androgens; C094335:H19 long non-coding RNA; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D062085:RNA, Long Noncoding; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D055517:snRNP Core Proteins