title: Stress exacerbates neuron loss and microglia proliferation in a rat model of excitotoxic lower motor neuron injury.
abstract: All individuals experience stress and hormones (e.g., glucocorticoids/GCs) released during stressful events can affect the structure and function of neurons. These effects of stress are best characterized for brain neurons; however, the mechanisms controlling the expression and binding affinity of glucocorticoid receptors in the spinal cord are different than those in the brain. Accordingly, whether stress exerts unique effects on spinal cord neurons, especially in the context of pathology, is unknown. Using a controlled model of focal excitotoxic lower motor neuron injury in rats, we examined the effects of acute or chronic variable stress on spinal cord motor neuron survival and glial activation. New data indicate that stress exacerbates excitotoxic spinal cord motor neuron loss and associated activation of microglia. In contrast, hypertrophy and hyperplasia of astrocytes and NG2+ glia were unaffected or were modestly suppressed by stress. Although excitotoxic lesions cause significant motor neuron loss and stress exacerbates this pathology, overt functional impairment did not develop in the relevant forelimb up to one week post-lesion. These data indicate that stress is a disease-modifying factor capable of altering neuron and glial responses to pathological challenges in the spinal cord.
authors.affiliation: Center for Brain and Spinal Cord Repair, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States; Department of Neuroscience, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States.
authors.forename: Denise A
authors.initials: DA
authors.lastname: Puga
authors.affiliation: Center for Brain and Spinal Cord Repair, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States; Department of Neuroscience, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States.
authors.forename: C Amy
authors.initials: CA
authors.lastname: Tovar
authors.affiliation: Center for Brain and Spinal Cord Repair, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States; Department of Neuroscience, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Guan
authors.affiliation: Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky 40536, United States.
authors.forename: John C
authors.initials: JC
authors.lastname: Gensel
authors.affiliation: Department of Neuroscience, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Lyman
authors.affiliation: Center for Brain and Spinal Cord Repair, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States; Department of Neuroscience, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States.
authors.forename: Dana M
authors.initials: DM
authors.lastname: McTigue
authors.affiliation: Center for Brain and Spinal Cord Repair, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States; Department of Neuroscience, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, United States.
authors.forename: Phillip G
authors.initials: PG
authors.lastname: Popovich
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology; D017628:Microglia / Q000187:drug effects / Q000502:physiology*; D009046:Motor Neurons / Q000187:drug effects / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology*; D013315:Stress, Psychological / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D018690:Excitatory Amino Acid Agonists; D018698:Glutamic Acid
keywords: Glucocorticoid receptor; Glucocorticoids; Glutamate; Hypothalamic–pituitary–adrenal axis; Microglia; Neuroinflammation; Spinal cord injury; Stress