title: Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.
abstract: The combination of porous graphitized carbon (PGC) liquid chromatography (LC) with mass spectrometric (MS) detection probably constitutes the most elaborate single stage analysis for isomer-specific N-glycan analysis. Here, we describe sample preparation and analysis procedures for the identification of released N-glycans using PGC-LC-ESI-MS and MS/MS.
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kolarich
authors.forename: Markus
authors.initials: M
authors.lastname: Windwarder
authors.forename: Kathirvel
authors.initials: K
authors.lastname: Alagesan
authors.forename: Friedrich
authors.initials: F
authors.lastname: Altmann
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D007536:Isomerism; D011134:Polysaccharides / Q000737:chemistry*; D016062:Porosity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D011134:Polysaccharides; D002244:Carbon