title: Antibacterial effect of electrospun polycaprolactone/polyethylene oxide/vancomycin nanofiber mat for prevention of periprosthetic infection and biofilm formation.
abstract: OBJECTIVE
  In this study, we developed a biocompatible and biodegradable vancomycin (VM)-eluting electrospun poly(ɛ-caprolactone) (PCL)/polyethylene oxide (PEO)/VM/PCL nanofiber mat. The purpose of this study was to evaluate the efficacy of this nanofiber mat for preventing methicillin-resistant Staphylococcus aureus (MRSA) biofilm on the ossicular prostheses and otitis media.
  
  
  METHODS
  Fabrication of VM eluting nanofiber mat was carried out using PCL and PEO. The release of VM was assessed by spectrophotometry in vitro. The antibacterial effect was evaluated by inhibition zone test and biofilm formation test in vitro. And prevention of MRSA infection by guinea pig was assessed by otoendoscopy, temporal bone CT and histopathologic examination in vivo.
  
  
  RESULTS
  VM released followed a triphasic pattern. VM eluting nanofiber mat prevented MRSA biofilm formation on the surface of ossicular prostheses regardless of materials in vitro, and MRSA otitis media in vivo. Otoendoscopy, temporal bone CT, and histopathologic findings revealed well ventilated middle ear.
  
  
  CONCLUSIONS
  From these results, VM eluting nanofiber mat may be a promising method for prevention of MRSA periprosthetic infection and biofilm formation.
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea. Electronic address: chulsavio@hanmail.net.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Jang
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.
authors.forename: Yong Beom
authors.initials: YB
authors.lastname: Cho
authors.affiliation: Ecobio Laboratory, Gwangju, South Korea.
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Jang
authors.affiliation: Department of Bio-Mechatronics, Sungkyunkwan University (SKKU), Suwon, South Korea.
authors.forename: Min Seong
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Bio-Mechatronics, Sungkyunkwan University (SKKU), Suwon, South Korea. Electronic address: gkimbme@skku.edu.
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects*; D004337:Drug Carriers; D006168:Guinea Pigs; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008876:Middle Ear Ventilation; D057139:Nanofibers; D009998:Ossicular Prosthesis / Q000382:microbiology*; D010033:Otitis Media / Q000517:prevention & control; D011091:Polyesters; D011092:Polyethylene Glycols; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D013203:Staphylococcal Infections / Q000517:prevention & control*; D014640:Vancomycin / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone; D011092:Polyethylene Glycols; D014640:Vancomycin
keywords: Biofilm; Nanofiber mat; Periprosthetic infection; Vancocmyin