title: Aichi Virus Positivity in HIV-1 Seropositive Children Hospitalized with Diarrheal Disease.
abstract: Aichi viruses (AiV) have been detected in patients with diarrheal diseases (DD). The aim of this study was to assess AiV infection rates in hospitalized children with DD, including 123 HIV-1 seropositive and 125 HIV-1 seronegative patients, in two public pediatric hospitals in Rio de Janeiro, Brazil. AiV was investigated by nested RT-PCR. The AiV-positive samples were also tested for specie A rotavirus, norovirus, astrovirus, enteric adenovirus and bocavirus in order to assess co-infections. AiV parcial genome sequencing and phylogenetic analyses were performed. AiV were detected in 9/123 (7.32%) of the HIV-1 seropositive subjects and 1/125 (0.8%) of the HIV seronegative patients with DD (p = 0.019). The phylogenetic analysis of positive samples disclosed that: i) 13 samples were characterized as genotype A, with one of them being from the HIV-1 seronegative patient; ii) one sample from a HIV-1 seropositive patient was characterized as genotype B. AiV genotype A was grouped into 3 genetic clusters. Data suggest that AiV may be an opportunistic pathogen infecting children with AIDS and DD.
authors.forename: Silvana Augusta Rodrigues
authors.initials: SA
authors.lastname: Portes
authors.forename: Eduardo
authors.initials: E
authors.lastname: de Mello Volotao
authors.forename: Tatiana Lundgren
authors.initials: TL
authors.lastname: Rose
authors.forename: Monica Simoes
authors.initials: MS
authors.lastname: Rocha
authors.forename: Maria da Penha
authors.initials: Mda P
authors.lastname: Trindade Pinheiro Xavier
authors.forename: Rosane Maria
authors.initials: RM
authors.lastname: de Assis
authors.forename: Alexandre Madi
authors.initials: AM
authors.lastname: Fialho
authors.forename: Myrna Santos
authors.initials: MS
authors.lastname: Rocha
authors.forename: Marize Pereira
authors.initials: MP
authors.lastname: Miagostovich
authors.forename: Jose Paulo
authors.initials: JP
authors.lastname: Gagliardi Leite
authors.affiliation: Oswaldo Cruz Foundation, Oswaldo Cruz Institute, Laboratory of Comparative and Environmental Virology, Pav. Helio & Peggy Pereira, Av. Brazil 4.365 - Manguinhos - 21040-360 - Rio de Janeiro - RJ - Brazil. sil@ioc.fiocruz.br.
authors.forename: Filipe Anibal
authors.initials: FA
authors.lastname: Carvalho-Costa
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D001938:Brazil; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D060085:Coinfection / Q000821:virology; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D005767:Gastrointestinal Diseases / Q000821:virology*; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000821:virology*; D015497:HIV-1; D006801:Humans; D007223:Infant; D052699:Kobuvirus / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000821:virology*