title: SIGN-R1 and complement factors are involved in the systemic clearance of radiation-induced apoptotic cells in whole-body irradiated mice.
abstract: Although SIGN-R1-mediated complement activation pathway has been shown to enhance the systemic clearance of apoptotic cells, the role of SIGN-R1 in the clearance of radiation-induced apoptotic cells has not been characterized and was investigated in this study. Our data indicated that whole-body γ-irradiation of mice increased caspase-3(+) apoptotic lymphocyte numbers in secondary lymphoid organs. Following γ-irradiation, SIGN-R1 and complements (C4 and C3) were simultaneously increased only in the mice spleen tissue among the assessed tissues. In particular, C3 was exclusively activated in the spleen. The delayed clearance of apoptotic cells was markedly prevalent in the spleen and liver of SIGN-R1 KO mice, followed by a significant increase of CD11b(+) cells. These results indicate that SIGN-R1 and complement factors play an important role in the systemic clearance of radiation-induced apoptotic innate immune cells to maintain tissue homeostasis after γ-irradiation.
authors.affiliation: Department of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Jin-Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: SoHee
authors.initials: S
authors.lastname: Loh
authors.affiliation: Department of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Eun-hee
authors.initials: EH
authors.lastname: Cho
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, 215-4, 75 Nowon gil Nowon-Gu, Seoul, 139-706, Republic of Korea.
authors.forename: Hyeong-Jwa
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-741, Republic of Korea.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Na
authors.affiliation: Regenerative Medicine Laboratory, Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Judee Grace E
authors.initials: JG
authors.lastname: Nemeno
authors.affiliation: Regenerative Medicine Laboratory, Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Jeong Ik
authors.initials: JI
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Yuhan College, Bucheon, Gyeonggi-do, 422-749, Republic of Korea.
authors.forename: Taek Joon
authors.initials: TJ
authors.lastname: Yoon
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, 215-4, 75 Nowon gil Nowon-Gu, Seoul, 139-706, Republic of Korea.
authors.forename: Minyoung
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon, 400-712, Republic of Korea.
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea; Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea. Electronic address: kangys1967@naver.com.
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects; D015815:Cell Adhesion Molecules / Q000502:physiology*; D003165:Complement System Proteins / Q000502:physiology*; D005720:Gamma Rays; D006801:Humans; D037181:Lectins, C-Type / Q000502:physiology*; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects; D008221:Lymphoid Tissue / Q000166:cytology / Q000528:radiation effects; D008264:Macrophages / Q000166:cytology / Q000528:radiation effects; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000502:physiology*; D014916:Whole-Body Irradiation; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D003165:Complement System Proteins; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
keywords: Apoptosis; Complement; SIGN-R1; Systemic clearance; γ-irradiation