title: Mimetic biomembrane-AuNPs-graphene hybrid as matrix for enzyme immobilization and bioelectrocatalysis study.
abstract: A hybrid composite constructed of phospholipids bilayer membrane, gold nanoparticles and graphene was prepared and used as matrices for microperoxidase-11 (MP11) immobilization. The direct electrochemistry and corresponding bioelectrocatalysis of the enzyme electrode was further investigated. Phospholipid bilayer membrane protected gold nanoparticles (AuNPs) were assembled on polyelectrolyte functionalized graphene sheets through electrostatic attraction to form a hybrid bionanocomposite. Owing to the biocompatible microenvironment provided by the mimetic biomembrane, microperoxidase-11 entrapped in this matrix well retained its native structure and exhibited high bioactivity. Moreover, the AuNPs-graphene assemblies could efficiently promote the direct electron transfer between the immobilized MP11 and the substrate electrode. The as-prepared enzyme electrode presented good direct electrochemistry and electrocatalytic responses to the reduction of hydrogen peroxide (H2O2). The resulting H2O2 biosensor showed a wide linear range (2.0×10(-5)-2.8×10(-4) M), a low detection limit (2.6×10(-6) M), good reproducibility and stability. Furthermore, this sensor was used for real-time detection of H2O2 dynamically released from the tumor cells MCF-7 in response to a pro-inflammatory stimulant.
authors.affiliation: College of Physics, Jilin University, Changchun, Jilin 130012, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Ren
authors.affiliation: College of Physics, Jilin University, Changchun, Jilin 130012, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; Department of Chemistry, Physics and Applied Mathematics, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA. Electronic address: jin.wang.1@stonybrook.edu.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. Electronic address: ekwang@ciac.jl.cn.
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry*; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010544:Peroxidases / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide; C002773:dimyristoylphosphatidylglycerol; D010544:Peroxidases; C021799:microperoxidase
keywords: Direct electrochemistry; Enzyme; Graphene nanocomposite; Hydrogen peroxide; Phospholipid