title: Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3.
abstract: The human renin gene has been widely known to be involved in essential hypertension (EH) pathogenesis. Genetic variant C-5434T of REN enhancer contributed to renin gene transcription and serum renin regulation. However, the mechanism associated with the transcription level changes remains unknown, and only a few reports exist that discussed serum renin levels on C-5434T of REN. Thus, this study aims to investigate the relationship between genetic variant C-5434T of REN enhancer and serum renin levels in Indonesian hypertensive patients. SNP of C-5434T was genotyped in 56 hypertensive patients by using RFLP. The data showed that serum renin is slightly higher in hypertensive patients with the TT genotype (39 ± 10.3) than patients with the CC genotype (33 ± 10.6) but the difference was not statistically significant (p = 0.35). Here, we also present a docking approach for predicting interaction between genetic variant -5434C/T and STAT3 (Signal Transducers and Activators Transcription 3), the predicted transcription factor that regulates renin gene enhancer. The results showed that STAT3-DNA allele T more favorably binds to DNA than STAT3-DNA allele C. These data suggest that the presence of genetic variant C-5434T has changed the binding pattern of STAT3 to REN enhancer. This is likely to influence STAT3 activity to stimulate the expression of renin gene in producing renin.
authors.affiliation: Biomedical Sciences, Faculty of Medicine, University of Brawijaya, Malang 65145, Indonesia.
authors.forename: Imama
authors.initials: I
authors.lastname: Maslahah
authors.affiliation: Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Saiful Anwar General Hospital, Malang 65145, Indonesia.
authors.forename: Mohammad Saifur
authors.initials: MS
authors.lastname: Rohman
authors.affiliation: Biology Department, Faculty of Mathematics and Sciences, University of Brawijaya, Malang 65145, Indonesia.
authors.forename: Nashi
authors.initials: N
authors.lastname: Widodo
authors.affiliation: Parasitology Department, Faculty of Medicine, University of Brawijaya, Malang 65145, Indonesia.
authors.forename: Agustina Tri
authors.initials: AT
authors.lastname: Endharti
authors.affiliation: Biology Department, Faculty of Mathematics and Sciences, University of Brawijaya, Malang 65145, Indonesia.
authors.forename: Didik
authors.initials: D
authors.lastname: Utomo