title: Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli.
abstract: For making a complex synthetic gene network function as designed, the parameters in the network have to be extensively tuned. In this study, a simple and general approach to rapidly tune gene networks in Escherichia coli was developed, which uses the hypermutable simple sequence repeats embedded in the spacer region between the ribosome binding site and the initiation codon. It was found that the change of sequence length and compositions of the repeated base pairs in 5'UTR contributes together to the changeable expression levels of the target gene. The mechanism of this phenomenon is that the transcriptional process makes greater impact on the expression level when compared to the translational process, which is utilized to successfully predict sample gene expression levels over a 50-fold range. The utility of the approach to regulate heterologous ecaA and pepc gene expression in the engineered E. coli for improving succinic acid yield and production has also been demonstrated. When the expression level of ecaA gene was 3.53-fold of the control and the expression level of pepc gene was 1.06-fold of the control, the highest yield of succinic acid and productivity were achieved, which was 0.87 g g(-1) and 2.01 g L(-1) h(-1), respectively.
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Qin
authors.affiliation: School of Bioengineering, Chongqing University, Chongqing, 400044, China.
authors.forename: Baoyun
authors.initials: B
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing, 210009, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Dong
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China. dwang@cqu.edu.cn.|State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing, 210009, China. dwang@cqu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
mesh_terms: D020121:5' Untranslated Regions; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D060847:Metabolic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D011994:Recombinant Proteins; D019802:Succinic Acid
keywords: Gene regulation; RBS; RNA polymerase holoenzyme; Succinic acid; Transcriptional level; Translational level