title: The durably resistant rice cultivar Digu activates defence gene expression before the full maturation of Magnaporthe oryzae appressorium.
abstract: Rice blast caused by the fungal pathogen Magnaporthe oryzae is one of the most destructive diseases worldwide. Although the rice-M. oryzae interaction has been studied extensively, the early molecular events that occur in rice before full maturation of the appressorium during M. oryzae invasion are unknown. Here, we report a comparative transcriptomics analysis of the durably resistant rice variety Digu and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to infection by M. oryzae (5, 10 and 20 h post-inoculation, prior to full development of the appressorium). We found that the transcriptional responses differed significantly between these two rice varieties. Gene ontology and pathway analyses revealed that many biological processes, including extracellular recognition and biosynthesis of antioxidants, terpenes and hormones, were specifically activated in Digu shortly after infection. Forty-eight genes encoding receptor kinases (RKs) were significantly differentially regulated by M. oryzae infection in Digu. One of these genes, LOC_Os08g10300, encoding a leucine-rich repeat RK from the LRR VIII-2 subfamily, conferred enhanced resistance to M. oryzae when overexpressed in rice. Our study reveals that a multitude of molecular events occur in the durably resistant rice Digu before the full maturation of the appressorium after M. oryzae infection and that membrane-associated RKs play important roles in the early response.
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Li
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
authors.forename: Mawsheng
authors.initials: M
authors.lastname: Chern
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Weilan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Bingtian
authors.initials: B
authors.lastname: Ma
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qin
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
authors.forename: Pamela
authors.initials: P
authors.lastname: Ronald
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.|Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
mesh_terms: D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified; D014157:Transcription Factors / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014157:Transcription Factors
keywords: Magnaporthe oryzae; blast disease; durable resistance; receptor kinase; rice; transcriptional profiling