title: [A cell membrane chromatography method for screening 5-HT receptor agonists from drug pair of Chuanxiong Rhizoma and Angelicae Dahuricae Radix].
abstract: Migraine is one of the common and frequently encountered diseases. The study proves that 5-hydroxytryptamine (5-HT) receptor, plays an important role in the occurrence of migraine. Rat striatum was used for preparation of the cell membrane stationary phase (CMSP) in our experiments. The cell membrane chromatography (CMC)-offline-HPLC system was applied to specifically recognize the components from the drug pair of Chuanxiong Rhizoma and Angelicae Dahuricae Radix, which interact with the receptors on CMSP. The dissociation equilibrium constant (KD) was measured in a rat striatum/CMC system, performed by continuously pumping sumatriptan, a 5-HT1D agonist, ranging from 2.42 x 10(-8) to 4.84 x 10(-7) mol · L(-1) through a CMC column, and the capacity factors (k') were recorded. The KD value obtained from the model was (4.59 ± 0.33) x 10(-6) mol · L(-1) for imperatorin, and the rat model of migraine induced by nitroglycerin was applied to validate the pharmacological effects of the drug pair. The results indicated that the CMC method could be a quick and efficient way for characterizing the drug-receptor interactions in vitro.
authors.forename: Hui
authors.initials: H
authors.lastname: Du
authors.forename: Nan
authors.initials: N
authors.lastname: Lv
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Deng
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008297:Male; D008881:Migraine Disorders / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D044346:Receptor, Serotonin, 5-HT1D / Q000737:chemistry; D017366:Serotonin Receptor Agonists / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D044346:Receptor, Serotonin, 5-HT1D; D017366:Serotonin Receptor Agonists; C534193:chuanxiong rhizome preparation