title: Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.
abstract: We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman(™) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10(2) to 10(5)CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.
authors.affiliation: Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. Electronic address: tlalani@idcrp.org.
authors.forename: Tahaniyat
authors.initials: T
authors.lastname: Lalani
authors.affiliation: Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
authors.forename: Michele D
authors.initials: MD
authors.lastname: Tisdale
authors.affiliation: Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Maguire
authors.affiliation: Independent Consultant, San Angelo, TX, USA.
authors.forename: Chansuda
authors.initials: C
authors.lastname: Wongsrichanalai
authors.affiliation: Enteric Diseases Department, Naval Medical Research Center, Silver Spring, MD, USA.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Riddle
authors.affiliation: Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Tribble
mesh_terms: D002167:Campylobacter / Q000302:isolation & purification; D003458:Cryptosporidium / Q000302:isolation & purification; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D005243:Feces / Q000382:microbiology* / Q000469:parasitology / Q000821:virology*; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D029322:Norovirus / Q000302:isolation & purification; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014195:Travel
keywords: PCR; Stool card; Travelers' diarrhea