title: [The influence of lentivirus-miRNA-184 on epithelial-mesenchcymal transition of human lens epithelial cells in vitro].
abstract: OBJECTIVE
  To analyze the influence of miRNA-184 on epithelial-mesenchymal transition (EMT) of human lens epithelial cells (HLEC) induced by TGF-beta2 in vitro.
  
  
  METHODS
  Experimental study. Recombinant plasmid of pL/IRES/GFP-miR-184 was constructed and used to produce the lentivirus. The lentivirus was used to transduce the HLEC which was in the process of EMT induced by transforming growth factor-β2 (TGF-β2). The real-time fluorescence quantitative PCR (QRT-PCR) was used to analyze E-cadherin (CDH1), α-smooth muscle actin (α-SMA), vimentin (VIM) expression at RNA levels during interval of 0 h, 6 h and 24 h after transduction, in comparison with that of control group. Statistical analysis method was single factor variance analysis.
  
  
  RESULTS
  The expression level of epithelial marker gene CDH1 in the miRNA-184 transduced group maintains relatively stable during 24h interval, while it goes down in the control group. The expression level of mesenchymal cell marker gene VIM, α-SMA in the miRNA-184 transduced group maintain relatively stable, while it goes up in the control group. The results of statistical analysis showed a statistically significant difference between miRNA-184 transduced group and control group (P<0.05).
  
  
  CONCLUSIONS
  miRNA-184 lentivirus-mediated HLEC can inhibit the occurrence of EMT.
authors.affiliation: Department of ophthalmology in Shanghai Jiangong Hospital, Shanghai 200083, China; Email: xuqinganan@126.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of ophthalmology in Shanghai Jiangong Hospital, Shanghai 200083, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of ophthalmology in Shanghai Jiangong Hospital, Shanghai 200083, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000032:analysis; D000704:Analysis of Variance; D015820:Cadherins / Q000032:analysis; D004847:Epithelial Cells / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology*; D016086:Lentivirus / Q000235:genetics*; D035683:MicroRNAs / Q000502:physiology*; D014161:Transduction, Genetic / Q000379:methods; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology*; D014746:Vimentin / Q000032:analysis
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D015820:Cadherins; D035683:MicroRNAs; D053781:Transforming Growth Factor beta2; D014746:Vimentin