title: Immunoaffinity Purification of Proteins.
abstract: This protocol entails a single-step, high-affinity purification of proteins using an immobilized antibody column.
authors.affiliation: Johns Hopkins School of Medicine, Baltimore, MD 21209, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Kavran
authors.affiliation: Johns Hopkins School of Medicine, Baltimore, MD 21209, USA. Electronic address: dleahy@jhmi.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Leahy
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods; D002021:Buffers; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D019163:Reducing Agents / Q000737:chemistry
chemical_list: D000906:Antibodies; D002021:Buffers; D011506:Proteins; D019163:Reducing Agents
keywords: Binding buffer; Elution buffer; Immunoaffinity purification; Immunoaffinty columns; Neutralization buffer; SDS-PAGE