title: In vitro gene expression within membrane-free coacervate protocells.
abstract: Cell-free gene expression of a fluorescent protein (mCherry) is demonstrated within the molecularly crowded matrix of a polysaccharide/polypeptide coacervate.
authors.affiliation: Centre for Protolife Research, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK. s.mann@bristol.ac.uk.
authors.forename: T-Y
authors.initials: TY
authors.lastname: Dora Tang
authors.forename: Dirk
authors.initials: D
authors.lastname: van Swaay
authors.forename: Andrew
authors.initials: A
authors.lastname: deMello
authors.forename: J L
authors.initials: JL
authors.lastname: Ross Anderson
authors.forename: Stephen
authors.initials: S
authors.lastname: Mann
mesh_terms: D058045:Artificial Cells / Q000737:chemistry / Q000378:metabolism*; D032701:Biomimetics; D003911:Dextrans / Q000737:chemistry; D015870:Gene Expression; D008164:Luminescent Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011107:Polylysine / Q000737:chemistry; D000097573:Red Fluorescent Protein
chemical_list: D003911:Dextrans; D008164:Luminescent Proteins; D011107:Polylysine; D000097573:Red Fluorescent Protein