title: [Analysis of sites of Toxoplasma gondii SAG2 gene in blood of HIV-positive people in Lincang City, Yunnan Province].
abstract: OBJECTIVE
  To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV-positive persons in Lincang City, Yunnan Province.
  
  
  METHODS
  Two segments of SAG2 gene of T. gondii from blood samples of HIV-positive persons in Lincang City were extracted and amplified by using the nested PCR method and the genotype was identified and compared with the standard strain (Type I) of Toxoplasma gondii.
  
  
  RESULTS
  Thirty-five SAG2 genes (241 bp) and 35 SAG2 genes (221 bp) of T. gondii were amplified from 170 blood samples of the HIV-positive people, and 4 of each case were selected and digested with enzyme, then 2 aim gene fragments of each case were chosen and compared with the standard strain (Type I) of T. gondii. The digestion of SAG2 gene (241 bp) showed the genotype of the blood samples was Type I or Type II, and the digestion of SAG2 gene (221 bp) confirmed that the genotype was Type I.
  
  
  CONCLUSIONS
  It is preliminarily confirmed that the genotype of T. gondii in blood of HIV-positive persons in Lincang City, Yunnan Province is Type I.
authors.forename: Yu-xi
authors.initials: YX
authors.lastname: Lia
authors.forename: Ling-juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Da-ping
authors.initials: DP
authors.lastname: Nie
authors.forename: Mi
authors.initials: M
authors.lastname: Luo
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: He
authors.forename: Li-jie
authors.initials: LJ
authors.lastname: Shen
mesh_terms: D000953:Antigens, Protozoan / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D005838:Genotype; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000097:blood / Q000235:genetics*; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D014123:Toxoplasmosis / Q000097:blood / Q000453:epidemiology / Q000209:etiology / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma