title: VENOM FROM THE ECTOPARASITIC WASP Habrobracon hebetor ACTIVATES CALCIUM-DEPENDENT DEGRADATION OF Galleria mellonella LARVAL HEMOCYTES.
abstract: Ectoparasitoids inject venom into hemolymph during oviposition. We determined the influence of envenomation by the parasitoid, Habrobracon hebetor, on the hemocytes of its larval host, Galleria mellonella. An increase in both intracellular Са(2+) content and phospholipase C activity of the host hemocytes was recorded during 2 days following envenomation by the parasitoid. The decreased hemocyte viability was detected 1, 2, and 24 h after the envenomation. Injecting of the crude venom (final protein concentration 3 μg/ml) into the G. mellonella larvae led to the reduced hemocyte adhesion. The larval envenomation caused a decrease in transmembrane potential of the hemocytes. These findings document the suppression of hemocytic immune effectors in the parasitized host larvae.
authors.affiliation: Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Kryukova
authors.affiliation: Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Chertkova
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Alexandra D
authors.initials: AD
authors.lastname: Semenova
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Yuri I
authors.initials: YI
authors.lastname: Glazachev
authors.affiliation: Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Slepneva
authors.affiliation: Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Victor V
authors.initials: VV
authors.lastname: Glupov
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion; D002470:Cell Survival; D006434:Hemocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D006458:Hemolymph / Q000166:cytology; D006790:Host-Parasite Interactions; D007814:Larva / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D008564:Membrane Potentials; D009036:Moths / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology*; D010738:Type C Phospholipases / Q000378:metabolism; D014862:Wasp Venoms / Q000378:metabolism* / Q000494:pharmacology; D014863:Wasps / Q000378:metabolism*
chemical_list: D014862:Wasp Venoms; D010738:Type C Phospholipases; D002118:Calcium
keywords: Habrobracon hebetor; free cytosolic calcium; hemocytes; transmembrane potential; venom