title: Isolation, Culture, Differentiation, and Nuclear Reprogramming of Mongolian Sheep Fetal Bone Marrow-Derived Mesenchymal Stem Cells.
abstract: We have characterized the differentiation potentiality and the developmental potential of cloned embryos of fetal bone marrow mesenchymal stem cells (BMSCs) isolated from Mongolian sheep. BMSCs were harvested by centrifuging after the explants method and the mononuclear cells obtained were cultured. The isolated BMSCs were uniform, with a fibroblast-like spindle or stellate appearance, and we confirmed expression of OCT4, SOX2, and NANOG genes at passage 3 (P3) by RT-PCR. We measured the growth of the passage 1, 5, and 10 cultures and found exponential growth with a population doubling time of 29.7±0.05 h. We cultured the P3 BMSCs in vitro under inductive environments and were able to induce them to undergo neurogenesis and form cardiomyocytes and adipocytes. Donor cells at passages 3-4 were used for nuclear transfer (NT). We found the BMSCs could be expanded in vitro and used as nuclear donors for somatic cell nuclear transfer (SCNT). Thus, BMSCs are an attractive cell type for large-animal autologous studies and will be valuable material for somatic cell cloning and future transgenic research.
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Su
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Yu
authors.initials: Y
authors.lastname: Ling
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Chunxia
authors.initials: C
authors.lastname: Liu
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Fanhua
authors.initials: F
authors.lastname: Meng
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Junwei
authors.initials: J
authors.lastname: Cao
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Huanmin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Zongzheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 1 College of Life Sciences, Inner Mongolia Agricultural University , Hohhot 010018, China .|2 Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region , Hohhot 010018, China .
authors.forename: Yanru
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D012756:Sheep / Q000196:embryology* / Q000502:physiology