title: Anti-TNF-α Activity of Brazilian Medicinal Plants and Compounds from Ouratea semiserrata.
abstract: Several plant species are used in Brazil to treat inflammatory diseases and associated conditions. TNF-α plays a pivotal role on inflammation, and several plant extracts have been assayed against this target, both in vitro and in vivo. The effect of 11 Brazilian medicinal plants on TNF-α release by LPS-activated THP-1 cells was evaluated. The plant materials were percolated with different solvents to afford 15 crude extracts, whose effect on TNF-α release was determined by ELISA. Among the evaluated extracts, only Jacaranda caroba (Bignoniaceae) presented strong toxicity to THP-1 cells. Considering the 14 non-toxic extracts, TNF-α release was significantly reduced by seven of them (inhibition > 80%), originating from six plants, namely Cuphea carthagenensis (Lythraceae), Echinodorus grandiflorus (Alismataceae), Mansoa hirsuta (Bignoniaceae), Ouratea semiserrata (Ochnaceae), Ouratea spectabilis and Remijia ferruginea (Rubiaceae). The ethanol extract from O. semiserrata leaves was fractionated over Sephadex LH-20 and RP-HPLC to give three compounds previously reported for the species, along with agathisflavone and epicatechin, here described for the first time in the plant. Epicatechin and lanceoloside A elicited significant inhibition of TNF-α release, indicating that they may account for the effect produced by O. semiserrata crude extract.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, campus Pampulha, Belo Horizonte, CEP 31.270-901, Brazil.|Divisão de Ciências Farmacêuticas, Fundação Ezequiel Dias, R. Conde Pereira Carneiro 80, Belo Horizonte, CEP 30.510-010, Brazil.
authors.forename: Priscilla R V
authors.initials: PR
authors.lastname: Campana
authors.affiliation: Departamento de Bioquímica, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, campus Pampulha, Belo Horizonte, CEP 31.270-901, Brazil.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Mansur
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, campus Pampulha, Belo Horizonte, CEP 31.270-901, Brazil.
authors.forename: Grasielle S
authors.initials: GS
authors.lastname: Gusman
authors.affiliation: Department of Pharmacognosy and Research Institute of Pharmaceutical Sciences, University of Mississippi, MS, 38677, USA.
authors.forename: Daneel
authors.initials: D
authors.lastname: Ferreira
authors.affiliation: Departamento de Bioquímica, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, campus Pampulha, Belo Horizonte, CEP 31.270-901, Brazil.
authors.forename: Mauro M
authors.initials: MM
authors.lastname: Teixeira
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, campus Pampulha, Belo Horizonte, CEP 31.270-901, Brazil.
authors.forename: Fernão C
authors.initials: FC
authors.lastname: Braga
mesh_terms: D029402:Bignoniaceae; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans; D043842:Ochnaceae; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D010946:Plants, Medicinal; D012997:Solvents; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D003911:Dextrans; D010936:Plant Extracts; D012997:Solvents; D014409:Tumor Necrosis Factor-alpha; C025614:sephadex
keywords: Brazilian medicinal plants; Ouratea semiserrata; TNF-α inhibition; epicatechin; lanceoloside A