title: Amplified solid-state electrochemiluminescence detection of cholesterol in near-infrared range based on CdTe quantum dots decorated multiwalled carbon nanotubes@reduced graphene oxide nanoribbons.
abstract: An amplified solid-state electrochemiluminescence (ECL) biosensor for detection of cholesterol in near-infrared (NIR) range was constructed based on CdTe quantum dots (QDs) decorated multiwalled carbon nanotubes@reduced graphene nanoribbons (CdTe-MWCNTs@rGONRs), which were prepared by electrostatic interactions. The CdTe QDs decorated on the MWCNTs@rGONRs resulted in the amplified ECL intensity by ~4.5 fold and decreased onset potential by ~100 mV. By immobilization of the cholesterol oxidase (ChOx) and NIR CdTe-MWCNTs@rGONRs on the electrode surface, a solid-state ECL biosensor for cholesterol detection was constructed. When cholesterol was added to the detection solution, the immobilized ChOx catalyzed the oxidation of cholesterol to generate H2O2, which could be used as the co-reactant in the ECL system of CdTe-MWCNTs@rGONRs. The as-prepared biosensor exhibited good performance for cholesterol detection including good reproducibility, selectivity, and acceptable linear range from 1 μM to 1mM with a relative low detection limit of 0.33 μM (S/N=3). The biosensor was successfully applied to the determination of cholesterol in biological fluid and food sample, which would open a new possibility for development of solid-state ECL biosensors with NIR emitters.
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huan
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Airong
authors.initials: A
authors.lastname: Fei
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Baijing
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Hanping
authors.initials: H
authors.lastname: Mao
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: wangkun@ujs.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D019187:Cadmium Compounds; D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D005504:Food Analysis / Q000379:methods; D006108:Graphite; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements; D008892:Milk / Q000737:chemistry; D053761:Nanocomposites; D037742:Nanotubes, Carbon; D045663:Quantum Dots; D015203:Reproducibility of Results; D013691:Tellurium
chemical_list: D019187:Cadmium Compounds; D037742:Nanotubes, Carbon; D006108:Graphite; D002784:Cholesterol; D013691:Tellurium; C028337:cadmium telluride
keywords: Amplified electrochemiluminescence; CdTe quantum dot; Graphene nanoribbon; Near-infrared; Solid-state cholesterol biosensor