title: In vitro characterization of a (E)-β-farnesene synthase from Matricaria recutita L. and its up-regulation by methyl jasmonate.
abstract: (E)-β-farnesene is a sesquiterpene semiochemical that is used extensively by both plants and animals for communication. This acyclic olefin is found in the essential oil of chamomile (Matricaria recutita) and was demonstrated that it could attract natural enemies to reduce cabbage aphids in the Chinese cabbage fields. However, little is known regarding the sequence and function of (E)-β-farnesene synthase in M. recutita. In this study, we reported a new full-length cDNA encoding (E)-β-farnesene synthase from M. recutita (Mr-βFS). The cDNA of Mr-βFS consisted of 2010bp including 1725bp of coding sequence encoding a protein of 574 amino acids with a molecular weight of 67kDa. The deduced amino acid sequence exhibits a considerably higher homology to βFS from Artemisia annua (about 92% identity) than to βFSs from other plants (about 20-40% identity). The recombinant enzyme, produced in Escherichia coli, catalyzed the formation of a single product, (E)-β-farnesene, from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis showed that Mr-βFS expression was highest in leaves and lowest in disk florets. The treatment of M. recutita with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of βFS gene and the content of (E)-β-farnesene in M. recutita. The transcriptional level of βFS gene was approximately 11.5-fold higher than the control sample and the (E)-β-farnesene emission level ranged from approximately from 0.082 to 0.695μg/g after 24h induction. Our results laid a solid foundation for later improving crop aphid resistance by transgenic technology and provided an important basic data for the regulation of valuable products from M. recutita.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Su
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Pan
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Hou
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, China. Electronic address: zhiwuxue239@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000595:Amino Acid Sequence; D003517:Cyclopentanes / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D031168:Matricaria / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011755:Pyrophosphatases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C072239:methyl jasmonate; D011755:Pyrophosphatases; C109459:beta-farnesene synthase
keywords: (E)-β-farnesene synthase; Matricaria recutita L; Methyl jasmonate; Sesquiterpene