title: Comparative performance of fetal goat tongue cell line ZZ-R 127 and fetal porcine kidney cell line LFBK-αvβ6 for Foot-and-mouth disease virus isolation.
abstract: The fetal goat tongue cell line ZZ-R 127 and the fetal porcine kidney cell line LFBK-α(v)β(6) have been reported to have high sensitivity to various Foot-and-mouth disease virus (FMDV) strains. The suitability of ZZ-R 127 cells for FMDV isolation not only from epithelial suspensions but also from other clinical samples has already been confirmed in a previous study. However, to our knowledge, the suitability of LFBK-α(v)β(6) cells has not been evaluated using clinical samples other than epithelial materials. In addition, both cell lines have never been compared, in terms of use for FMDV isolation, under the same conditions. Therefore, in the current study, the virus isolation rates of both cell lines were compared using clinical samples collected from animals infected experimentally with FMDV. Viruses were successfully isolated from clinical samples other than epithelial suspensions for both cell lines. The virus isolation rates for the 2 cell lines were not significantly different. The Cohen kappa coefficients between the virus isolation results for both cell lines were significantly high. Taken together, these results confirmed the suitability of LFBK-α(v)β(6) cells for FMDV isolation from clinical samples other than epithelial suspensions. The levels of susceptibility of both cell lines to FMDV isolation were also confirmed to be almost the same.
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Fukai
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Morioka
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishi
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Kitano
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Yamazoe
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Japan kannot@affrc.go.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Kanno
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line / Q000821:virology; D005333:Fetus / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000517:prevention & control / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D006041:Goats; D007668:Kidney / Q000821:virology; D013552:Swine; D014059:Tongue / Q000821:virology
keywords: Cell line; Foot-and-mouth disease virus; virus isolation