title: [Effect of tamibarotene on innate immune response in the model of Porphyromonas gingivalis induced periodontitis].
abstract: OBJECTIVE
  To investigate the effect of Am80 on innate immune response of Porphyromonas gingivalis (Pg) W83-induced periodontitis in mice.
  
  
  METHODS
  Twenty-five mice were randomly divided into five groups, control group, PgW83-induced periodontitis group (periodontitis), Am80 (10 µg/d) treatment group (low-dose group), Am80 (50 µg/d) treatment group (middle-dose group), Am80 (100 µg/d) treatment group (high-dose group). The distance of alveolar bone resorption in each mouse was observed and measured by a dissecting microscope. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of serum anti-Pg specific IgG. The mRNA expression of interferon-γ (IFN-γ) and interleuking-12 (IL-12) in gingival tissues, draining lymph node and spleen were detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The measurement data were statistically analyzed.
  
  
  RESULTS
  The resorption rate in Am80 group [(121 ± 10)%] and high-dose of Am80 group [(108 ± 8)%] was significantly different with that in periodontitis mice [(133 ± 10)% ] (P < 0.05). The serum levels of anti-Pg specific IgG of the Am80 groups of different doses (0.437 ± 0.083, 0.566 ± 0.012, and 0.386 ± 0.078) were significantly lower than that of the periodontitis group (1.151 ± 0.433) (P < 0.001). Real-time quantitative PCR assay showed that after Am80 treatment, the IL-12 mRNA levels in the gingival tissues, lymph nodes and spleen of mice were reduced to 1.107 ± 0.088, 0.806 ± 0.220, and 0.668 ± 0.756, which were all significantly different with those in periodontitis (P < 0.01). Similarly, the relative expression of IFN-γ mRNA levels in gingival tissue, lymph nodes and spleen of mice were reduced to 8.898 ± 0.427, 16.654 ± 5.995, and 1.482 ± 0.033, which were significantly different with periodontitis (P < 0.001).
  
  
  CONCLUSIONS
  Am80 can reduce the extent of inflammation and alleviate alveolar bone resorption by modulating innate immune response.
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Dixin
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Kebei
authors.initials: K
authors.lastname: Gao
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang 110004, China; Email: xiaoping_ba@126.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lin
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology; D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D005881:Gingiva / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects*; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D018664:Interleukin-12 / Q000097:blood; D051379:Mice; D010518:Periodontitis / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D013154:Spleen / Q000276:immunology; D013764:Tetrahydronaphthalenes / Q000494:pharmacology*
chemical_list: D001565:Benzoates; D007074:Immunoglobulin G; D012333:RNA, Messenger; D013764:Tetrahydronaphthalenes; C061133:tamibarotene; D018664:Interleukin-12; D007371:Interferon-gamma