title: Preconcentration and detection of mercury with bioluminescent bioreporter E. coli ARL1.
abstract: Cell wall envelopes treated with sodium hydroxide and spray-dried were used as mercury sorbents. The sorbent having sorption capacity 17.7 ± 0.1 μmol/g determined was employed for preconcentration of mercury containing 1-10 ng/L. After preconcentration, bioavailable mercury was detected in samples of soil, stream, and tap water via induction of bioluminescence of E. coli ARL1. Iron and manganese at concentrations of tenth microgram per liter interfered bioluminescence detection of mercury. In tap water was detected semiquantitatively 0.127 ± 0.1 nmol/L by the induction of bioluminescence of E. coli ARL1 in medium with tryptone after preconcentration using a method of standard addition.
authors.affiliation: Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Rozvojova 135, 16502, Prague 6, Czech Republic. solovyev@icpf.cas.cz.
authors.forename: Andrey I
authors.initials: AI
authors.lastname: Solovyev
authors.affiliation: Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Rozvojova 135, 16502, Prague 6, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Koštejn
authors.affiliation: Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Rozvojova 135, 16502, Prague 6, Czech Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Kuncova
authors.affiliation: Department of Biotechnology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague 6, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Dostálek
authors.affiliation: Institute of Geology of the Czech Academy of Sciences, Rozvojova 135, 16502, Prague 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Rohovec
authors.affiliation: Institute of Geology of the Czech Academy of Sciences, Rozvojova 135, 16502, Prague 6, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Navrátil
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D008163:Luminescent Measurements; D008628:Mercury / Q000032:analysis*; D012987:Soil / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D012987:Soil; D014867:Water; D008628:Mercury
keywords: Bioreporters; Biosorbents; Mercury detection; Mercury sorption