title: Synthesis of Unprotected Linear or Cyclic O-Acyl Isopeptides in Water Using Bis(2-sulfanylethyl)amido Peptide Ligation.
abstract: SEA ligation proceeds chemoselectively at pH 3, i.e., at a pH where the O-acyl isopeptides are protected by protonation. This property was used for synthesizing unprotected O-acyl isopeptides in water, starting from peptide segments which are easily accessible by the Fmoc SPPS.
authors.affiliation: UMR CNRS 8161, Université Lille, Pasteur Institute of Lille 59021 Lille, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Desmet
authors.affiliation: UMR CNRS 8161, Université Lille, Pasteur Institute of Lille 59021 Lille, France.
authors.forename: Mindaugas
authors.initials: M
authors.lastname: Pauzuolis
authors.affiliation: UMR CNRS 8161, Université Lille, Pasteur Institute of Lille 59021 Lille, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Boll
authors.affiliation: UMR CNRS 8161, Université Lille, Pasteur Institute of Lille 59021 Lille, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.affiliation: UMR CNRS 8161, Université Lille, Pasteur Institute of Lille 59021 Lille, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Raibaut
authors.affiliation: UMR CNRS 8161, Université Lille, Pasteur Institute of Lille 59021 Lille, France.
authors.forename: Oleg
authors.initials: O
authors.lastname: Melnyk
mesh_terms: D000215:Acylation; D008026:Ligation; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D010455:Peptides; D014867:Water