title: Fibulin-5 regulates keloid-derived fibroblast-like cells through integrin beta-1.
abstract: OBJECTIVE
  Keloid scar is pathological tissue that appears after skin injury, and that is more aggressive than hypertrophic scars. Keloid scars are characterized by increased proliferation of fibroblast-like cells (FLCs) and the accumulation of extracellular matrix, mainly collagen. Fibulin-5, a glycoprotein secreted by many cell types, is a component of the extracellular matrix. We investigated the effect of fibulin-5 on the adhesion and proliferation of FLCs derived from keloid scars and the role of integrin beta-1 in these activities.
  
  
  METHODS
  Fibroblast-like cells were isolated from six keloid scars and cultured on plates coated with fibulin-5 or with gelatin. Cells were incubated for 72-96 h to examine proliferation rates and incubated for 240 min, with washings at 20, 40, 60, 90, 120, 180 min, to assess adhesion rates. To examine the role of integrin beta-1, the anti-human integrin beta-1 (CD29) antibody was added to the culture medium.
  
  
  RESULTS
  Fibroblast-like cells from keloids cultured on a fibulin-5-coated surface showed a significantly reduced proliferation rate and a delayed adhesion rate, compared to cells cultured on gelatin-coated dishes. Adherence of these cells to fibulin-5 pre-coated wells was significantly reduced in the presence of anti-human integrin beta-1 (CD29) antibodies. Our current findings are similar to previously observed reduced proliferation in vascular smooth muscle cells overexpressing fibulin-5. We did not test the effects of fibulin-5 on normal fibroblasts.
  
  
  CONCLUSIONS
  This study demonstrates the pivotal role of the extracellular protein, fibulin-5, on the adhesion and proliferation of human keloid-derived cells, through binding to integrin beta-1.
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Furie
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Shteynberg
authors.affiliation: Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.|Department of Plastic Surgery, Ramabam - Health Care Campus, P.O.B 9602, Haifa 3109601, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Elkhatib
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Perry
authors.affiliation: Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.|Department of Plastic Surgery, Ramabam - Health Care Campus, P.O.B 9602, Haifa 3109601, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Ullmann
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Feferman
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Preis
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: M Y
authors.initials: MY
authors.lastname: Flugelman
authors.affiliation: Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34361, Israel.|Ruth and Bruce Rappaport Faculty of Medicine, Technion IIT, P.O.B. 9649, Efron Street, Bat Galim, Haifa 31096, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Tzchori
mesh_terms: D000328:Adult; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D019012:Integrin beta1 / Q000502:physiology*; D007627:Keloid / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D016326:Extracellular Matrix Proteins; C492373:FBLN5 protein, human; D019012:Integrin beta1
keywords: cell culture; fibulin-5; integrin beta-1; keloid; microbiology; skin physiology/structure