title: Estimation of the presence of unmetabolized dialkyl phthalates in untreated human urine by an on-line miniaturized reliable method.
abstract: At present, human exposure to dialkyl phthalates is assessed through urinary measurement of their metabolites due mainly to contamination in their analysis by their ubiquitous presence. An on-line miniaturized method and the processing of the untreated urine samples have been the key factors for minimizing contamination and achieving unbiased results. Di(2-ethylhexyl) (DEHP), diethyl (DEP), dibutyl (DBP) and mono-ethylhexyl (MEHP) phthalates in urine samples have been included in the study; MEHP as metabolite of the main dialkyl phthalate such as DEHP. On-line in-tube solid-phase microextraction (IT-SPME)-capillary liquid chromatography (CapLC) with diode array detection (DAD) is employed. The detection limits (LODs) achieved in urine were between 0.5 and 1.5 μg/L. Eighteen urines were processed. DBP and DEHP were found in nine and five samples, respectively and DEP in three of them. MEHP was only detected in one of the eighteen samples analyzed.
authors.affiliation: MINTOTA Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Jornet-Martínez
authors.affiliation: MINTOTA Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Antón-Soriano
authors.affiliation: MINTOTA Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain. Electronic address: pilar.campins@uv.es.
authors.forename: P
authors.initials: P
authors.lastname: Campíns-Falcó
mesh_terms: D004781:Environmental Exposure / Q000032:analysis*; D004785:Environmental Pollutants / Q000652:urine*; D006801:Humans; D010795:Phthalic Acids / Q000652:urine*; D016482:Urinalysis / Q000379:methods
chemical_list: D004785:Environmental Pollutants; D010795:Phthalic Acids; C032279:phthalic acid
keywords: Capillary liquid chromatography; Dialkyl phthalates; In-tube solid-phase microextraction (IT-SPME); Urine analysis