title: Arabidopsis dynamin-related protein 1E in sphingolipid-enriched plasma membrane domains is associated with the development of freezing tolerance.
abstract: The freezing tolerance of Arabidopsis thaliana is enhanced by cold acclimation, resulting in changes in the compositions and function of the plasma membrane. Here, we show that a dynamin-related protein 1E (DRP1E), which is thought to function in the vesicle trafficking pathway in cells, is related to an increase in freezing tolerance during cold acclimation. DRP1E accumulated in sphingolipid and sterol-enriched plasma membrane domains after cold acclimation. Analysis of drp1e mutants clearly showed that DRP1E is required for full development of freezing tolerance after cold acclimation. DRP1E fused with green fluorescent protein was visible as small foci that overlapped with fluorescent dye-labelled plasma membrane, providing evidence that DRP1E localizes non-uniformly in specific areas of the plasma membrane. These results suggest that DRP1E accumulates in sphingolipid and sterol-enriched plasma membrane domains and plays a role in freezing tolerance development during cold acclimation.
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Anzu
authors.initials: A
authors.lastname: Minami
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tominaga
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Akari
authors.initials: A
authors.lastname: Furuto
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Kondo
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550, Japan.
authors.forename: Matsuo
authors.initials: M
authors.lastname: Uemura
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D017360:Arabidopsis / Q000254:growth & development*; D029681:Arabidopsis Proteins; D002462:Cell Membrane / Q000378:metabolism*; D057773:Cold Shock Proteins and Peptides / Q000502:physiology*; D020558:GTP Phosphohydrolases; D013107:Sphingolipids
chemical_list: D029681:Arabidopsis Proteins; D057773:Cold Shock Proteins and Peptides; D013107:Sphingolipids; D020558:GTP Phosphohydrolases; C513746:EDR3 protein, Arabidopsis
keywords: Arabidopsis thaliana; cold acclimation; detergent-resistant plasma membrane; dynamin-related protein; freezing tolerance; microdomain; plasma membrane