title: Interlaboratory Study of ELISA Kits for the Detection of Egg and Milk Protein in Processed Foods.
abstract: The labeling of seven specific allergenic ingredients (egg, milk, wheat, buckwheat, peanut, shrimp, and crab) is mandatory in Japan. To ensure proper labeling, two kinds of ELISA kits using polyclonal antibodies have been developed. However, we developed two novel ELISA kits using monoclonal antibodies with improved specificity, the Allergeneye ELISA Egg (AE-Egg) and Allergeneye ELISA Milk (AE-Milk) Kits, to detect egg and milk proteins in processed foods, respectively. Five types of processed food containing 10 mg/kg of egg or milk soluble protein were prepared for an interlaboratory study of the performance of these kits. The kits showed a relatively high reproducibility level of interlaboratory precision (AE-Egg RSDR, 3.7-5.7%; AE-Milk RSDR, 6.8-10.5%) and satisfied the recovery rate stipulated by Japanese guidelines (AE-Egg, 61.6-89.3%; AE-Milk, 52.1-67%) for all processed foods. Our results suggest that the AE-Egg and AE-Milk Kits are precise and reliable tools for detecting egg or milk proteins in processed foods.
authors.affiliation: PRIMA Meat Packers, Ltd, 635 Nakamukaihara, Tsuchiura, Ibaraki 300-0841, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kato
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yagi
authors.forename: Ayako
authors.initials: A
authors.lastname: Kato
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Masanobu
authors.initials: M
authors.lastname: Akimoto
authors.forename: Keizo
authors.initials: K
authors.lastname: Arihara
mesh_terms: D002138:Calibration; D003611:Dairy Products / Q000032:analysis; D004527:Egg Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D008894:Milk Proteins / Q000032:analysis*; D011208:Powders; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D004527:Egg Proteins; D008894:Milk Proteins; D011208:Powders; D011933:Reagent Kits, Diagnostic