title: An acoustofluidic sputum liquefier.
abstract: We demonstrate the first microfluidic-based on-chip liquefaction device for human sputum samples. Our device is based on an acoustofluidic micromixer using oscillating sharp edges. This acoustofluidic sputum liquefier can effectively and uniformly liquefy sputum samples at a throughput of 30 μL min(-1). Cell viability and integrity are maintained during the sputum liquefaction process. Our acoustofluidic sputum liquefier can be conveniently integrated with other microfluidic units to enable automated on-chip sputum processing and analysis.
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA. junhuang@psu.edu.
authors.forename: Po-Hsun
authors.initials: PH
authors.lastname: Huang
authors.forename: Liqiang
authors.initials: L
authors.lastname: Ren
authors.forename: Nitesh
authors.initials: N
authors.lastname: Nama
authors.forename: Sixing
authors.initials: S
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Xianglan
authors.initials: X
authors.lastname: Yao
authors.forename: Rosemarie A
authors.initials: RA
authors.lastname: Cuento
authors.forename: Cheng-Hsin
authors.initials: CH
authors.lastname: Wei
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Chen
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Xie
authors.forename: Ahmad Ahsan
authors.initials: AA
authors.lastname: Nawaz
authors.forename: Yael G
authors.initials: YG
authors.lastname: Alevy
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Holtzman
authors.forename: J Philip
authors.initials: JP
authors.lastname: McCoy
authors.forename: Stewart J
authors.initials: SJ
authors.lastname: Levine
authors.forename: Tony Jun
authors.initials: TJ
authors.lastname: Huang
mesh_terms: D002470:Cell Survival; D004804:Eosinophils; D004867:Equipment Design; D005434:Flow Cytometry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009504:Neutrophils; D013010:Sonication; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D013183:Sputum / Q000166:cytology* / Q000502:physiology*