title: [Establishment and evaluation of identification method for Yersinia pestis and Yersinia pseudotuberculosis].
abstract: OBJECTIVE
  To establish a gene identification method of Yersinia pestis and Yersinia pseudotuberculosis for plague surveillance.
  
  
  METHODS
  According to the specific genomic sequences of Y. pestis and Y. pseudotuberculosis, i.e. "pestis Island (PeI)" and "pseudotuberculosis Island (PsI)" and the published genomic sequences of 12 strains of Y. pestis and 4 strains of Y. pseudotuberculosis, the specific identification primers of these sequences were designed.
  
  
  RESULTS
  A total of 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis and other intestinal bacteria strains were tested with PCR. Of the 5 pairs of Y. pestis identification primers, PeI2 and PeI11 were specific for Y. pestis. Besides Y. pestis, the primers PeI1, PeI3 and PeI12 could detect part of 57 Y. pseudotuberculosis strains. Of the 5 pairs of Y. pseudotuberculosis identification primers, PsI1 could detect all the 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis. PsI7, PsI16, PsI18 and PsI19 were specific for Y. pseudotuberculosis.
  
  
  CONCLUSIONS
  The primers PsI1, PeI 2 and PeI11, PsI7, PsI16, PsI18 and PsI19 can be used in the rapid identification of Y. pestis and Y. pseudotuberculosis, which can be also used to explore the circulation of atypical Y. pestis in quiescent plague foci.
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Shi
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Mei
authors.affiliation: Yiwu Prefecture Center for Disease Control and Prevention.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Chen
authors.affiliation: Longquan County Center for Disease Control and Prevention.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Mei
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Dazhi
authors.initials: D
authors.lastname: Jin
authors.affiliation: Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention.
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention.
authors.forename: Yumeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Wenzhou Prefecture Center for Disease Control and Prevention.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention.
authors.forename: Dongzheng
authors.initials: D
authors.lastname: Yu
authors.affiliation: Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention; Email: xialianxu@icdc.cn.
authors.forename: Lianxu
authors.initials: L
authors.lastname: Xia
mesh_terms: D001483:Base Sequence; D002681:China / Q000453:epidemiology; D017931:DNA Primers; D023281:Genomics; D006801:Humans; D010930:Plague / Q000175:diagnosis* / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011159:Population Surveillance / Q000379:methods*; D015010:Yersinia pestis / Q000235:genetics*; D015011:Yersinia pseudotuberculosis / Q000235:genetics*
chemical_list: D017931:DNA Primers