title: MreB-Dependent Inhibition of Cell Elongation during the Escape from Competence in Bacillus subtilis.
abstract: During bacterial exponential growth, the morphogenetic actin-like MreB proteins form membrane-associated assemblies that move processively following trajectories perpendicular to the long axis of the cell. Such MreB structures are thought to scaffold and restrict the movement of peptidoglycan synthesizing machineries, thereby coordinating sidewall elongation. In Bacillus subtilis, this function is performed by the redundant action of three MreB isoforms, namely MreB, Mbl and MreBH. mreB and mbl are highly transcribed from vegetative promoters. We have found that their expression is maximal at the end of exponential phase, and rapidly decreases to a low basal level upon entering stationary phase. However, in cells developing genetic competence, a stationary phase physiological adaptation, expression of mreB was specifically reactivated by the central competence regulator ComK. In competent cells, MreB was found in complex with several competence proteins by in vitro pull-down assays. In addition, it co-localized with the polar clusters formed by the late competence peripheral protein ComGA, in a ComGA-dependent manner. ComGA has been shown to be essential for the inhibition of cell elongation characteristic of cells escaping the competence state. We show here that the pathway controlling this elongation inhibition also involves MreB. Our findings suggest that ComGA sequesters MreB to prevent cell elongation and therefore the escape from competence.
authors.affiliation: INRA, UMR 1319 Micalis, Jouy-en-Josas, France; AgroParisTech, UMR 1319 Micalis, Jouy-en-Josas, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mirouze
authors.affiliation: INRA, UMR 1319 Micalis, Jouy-en-Josas, France; AgroParisTech, UMR 1319 Micalis, Jouy-en-Josas, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Ferret
authors.affiliation: INRA, UMR 1319 Micalis, Jouy-en-Josas, France; AgroParisTech, UMR 1319 Micalis, Jouy-en-Josas, France.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Yao
authors.affiliation: INRA, UMR 1319 Micalis, Jouy-en-Josas, France; AgroParisTech, UMR 1319 Micalis, Jouy-en-Josas, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Chastanet
authors.affiliation: INRA, UMR 1319 Micalis, Jouy-en-Josas, France; AgroParisTech, UMR 1319 Micalis, Jouy-en-Josas, France.
authors.forename: Rut
authors.initials: R
authors.lastname: Carballido-López
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D061269:DNA Transformation Competence; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D020033:Protein Isoforms