title: Viral Infection-Induced Differential Expression of LncRNAs Associated with Collagen in Mouse Placentas and Amniotic Sacs.
abstract: OBJECTIVE
  We have previously determined that long non-coding RNAs (lncRNAs) are differentially expressed in preterm premature rupture of membranes (PPROM) and hypothesized that the collagenolysis ubiquitin-proteasome system may be activated by infection and inflammation. However, direct evidence of the involvement of lncRNAs in transcriptional and posttranscriptional regulation of the infection-triggered alteration of collagen is lacking.
  
  
  METHODS
  A previously developed mouse model with MHV68 viral infection was assessed to determine whether viral infection may induce differential expression of lncRNAs in mouse placentas and amniotic sacs.
  
  
  RESULTS
  Differential expression of lncRNAs that are associated with collagen was found in HMV68 viral-infected, compared to non-infected, mouse placentas and amniotic sacs. Differential expression of messenger RNAs (mRNAs) of collagen was also documented.
  
  
  CONCLUSIONS
  Our data demonstrate, for the first time, that viral infection may induce the differential expression of lncRNAs that are associated with collagen. Based on this finding, we propose that lncRNA may have involved in regulating of infection-induced collagen transcription.
authors.affiliation: Center of Translational Medicine for Maternal and Children's Health, Lianyungang Maternal and Children's Hospital, Lianyungang, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Obstetrics Gynecology and Reproductive Sciences, Reproductive Immunology Unit, School of Medicine, Yale University, New Haven, CT, USA.
authors.forename: Gil
authors.initials: G
authors.lastname: Mor
authors.affiliation: Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA.
authors.forename: Weina
authors.initials: W
authors.lastname: Ju
authors.affiliation: Hunter College High School, New York, NY, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Center of Translational Medicine for Maternal and Children's Health, Lianyungang Maternal and Children's Hospital, Lianyungang, Jiangsu, China.
authors.forename: Xiucui
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Obstetrics Gynecology and Reproductive Sciences, Reproductive Immunology Unit, School of Medicine, Yale University, New Haven, CT, USA.
authors.forename: Paulomi Bole
authors.initials: PB
authors.lastname: Aldo
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA.
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Jenkins
authors.affiliation: Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA.
authors.forename: William T
authors.initials: WT
authors.lastname: Brown
authors.affiliation: Center of Translational Medicine for Maternal and Children's Health, Lianyungang Maternal and Children's Hospital, Lianyungang, Jiangsu, China.|Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA.
authors.forename: Nanbert
authors.identifier: http://orcid.org/0000-0002-3242-5308
authors.initials: N
authors.lastname: Zhong
mesh_terms: D000650:Amnion / Q000378:metabolism* / Q000821:virology; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism / Q000821:virology; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D051379:Mice; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism* / Q000821:virology; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins
chemical_list: D062085:RNA, Long Noncoding; D014759:Viral Envelope Proteins; C089386:glycoprotein B, murine gammaherpesvirus 68; D003094:Collagen
keywords: Collagen; differential expression; long non-coding RNA; pathogenic hypothesis; pregnancy