title: Association of DLA-DQB1 alleles with exocrine pancreatic insufficiency in Pembroke Welsh Corgis.
abstract: Exocrine pancreatic insufficiency (EPI) is a digestive disorder resulting from the insufficient secretion of enzymes from the pancreas. In dogs, this condition is often attributed to pancreatic acinar atrophy, wherein the enzyme-producing acinar cells are believed to be destroyed through an autoimmune process. Although EPI affects many diverse breeds, to date, molecular studies have been limited to the German Shepherd dog. A recent study of major histocompatibility genes in diseased and healthy German Shepherd dogs identified both risk and protective haplotypes. Herein, we genotyped DLA-DQB1 in Pembroke Welsh Corgis to determine whether dog leukocyte antigen alleles contribute to the pathogenesis of EPI across dog breeds. We evaluated 14 affected and 43 control Pembroke Welsh Corgis, which were selected based on an age of onset similar to German Shepherd dogs. We identified one protective allele (odds ratio = 0.13, P-value = 0.044) and one risk allele (odds ratio = 3.8, P-value = 0.047). As in German Shepherd dogs, the risk allele is a duplication of DLA-DQB1 (alleles DQB1*013:03 and 017:01); however, Pembroke Welsh Corgis have acquired a single polymorphism on DQB1*017:01. Thus, the DLA-DQB1 duplication is a risk allele for EPI in at least two breeds.
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Evans
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Tsai
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA.
authors.forename: A N
authors.initials: AN
authors.lastname: Starr-Moss
authors.affiliation: Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX, 77843, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Steiner
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Clark
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D010188:Exocrine Pancreatic Insufficiency / Q000235:genetics / Q000662:veterinary*; D020440:Gene Duplication; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D015395:Histocompatibility Antigens Class I
keywords: autoimmune; canine; dog leukocyte antigen; duplication; major histocompatibility complex; pancreatic acinar atrophy