title: Expression of plzfa in embryo and adult of medaka Oryzias latipes.
abstract: In this study, a homologous gene named plzfa was identified and characterized in medaka Oryzias latipes. Oryzias latipes plzfa was detected in all the tissues including brain, gill, muscle, liver, intestine, kidney, spleen, testis and ovary using reverse transcriptase (RT)-PCR. plzfa was detected in the oocytes of the ovary and in the spermatogonia and somitic cells of the testis by in situ hybridization. plzfa had a maternal origin with continuous and dynamic expression during embryonic development. plzfa was observed in the brain, neural rod and sensor organs including the eyes, ears and nose during embryogenesis. plzfa was also detected in the neural crest, somite, pectoral fin, intestine and skin. These results indicate that plzfa is a pleiotropic gene that may play major roles in various tissues.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Zhao
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Feng
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: M M
authors.initials: MM
authors.lastname: Guo
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: P
authors.initials: P
authors.lastname: Chang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: C
authors.initials: C
authors.lastname: Qi
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: X P
authors.initials: XP
authors.lastname: Zhong
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: Q C
authors.initials: QC
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D020539:Sequence Analysis, Protein; D013093:Spermatogonia / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D029941:Fish Proteins; D051741:Kruppel-Like Transcription Factors
keywords: embryogenesis; gene expression; gonad; in situ hybridization; real time RT-PCR; tissue distribution