title: 5-Formylcytosine can be a stable DNA modification in mammals.
abstract: 5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.
authors.affiliation: 1] Department of Chemistry, University of Cambridge, Cambridge, UK. [2] Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Bachman
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Santiago
authors.initials: S
authors.lastname: Uribe-Lewis
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
authors.affiliation: Babraham Institute, Babraham, UK.
authors.forename: Heather E
authors.initials: HE
authors.lastname: Burgess
authors.affiliation: Babraham Institute, Babraham, UK.
authors.forename: Mario
authors.identifier: 0000000339199689
authors.initials: M
authors.lastname: Iurlaro
authors.affiliation: 1] Babraham Institute, Babraham, UK. [2] Wellcome Trust Sanger Institute, Hinxton, UK.
authors.forename: Wolf
authors.initials: W
authors.lastname: Reik
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Adele
authors.initials: A
authors.lastname: Murrell
authors.affiliation: 1] Department of Chemistry, University of Cambridge, Cambridge, UK. [2] Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Shankar
authors.initials: S
authors.lastname: Balasubramanian
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism* / Q000031:analogs & derivatives; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D018507:Gene Expression Regulation, Developmental; D006207:Half-Life; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000378:metabolism
chemical_list: C560973:5-formylcytosine; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases