title: [Effect of SIRT6/NF-κB signal axis in delaying hematopoietic stem/progenitor cell senescence with ginsenoside Rg1].
abstract: OBJECTIVE
  To investigate the effect of SIRT6/NF-κB signal axis in delaying hematopoietic stem/progenitor cell senescence with ginsenoside Rg1, in order to provide theatrical and experimental basis for looking for methods for delaying HSC senescence.
  
  
  METHODS
  Sca-1 + HSC/HPC was isolated by magnetic cell sorting (MACS) and divided into five groups: the normal control group, the aging group, the positive control group, the Rg1 anti-senescence group, and the Rg1-treated group. Senescence-associated β-galactosidase (SA-β-Gal) staining, cell cycle analysis and hemopoietic progenitor cell mix (CFU-Mix) were adopted to determine the effect Rg1 in delaying or treating Sca-1 + HSC/HPC senescence biology. The mRNA and protein of senescence regulation molecules SIRT6 and NF-KB were examined by realtime fluorescence quantitative PCR (FQ-PCR) and western blotting.
  
  
  RESULTS
  Compared with the senescence group, the Rg1 anti-senescence group and the Rg1-treated group showed lower percentage in SA-β-Gal-stained positive cells, decreased cell proportion in G1 phase, increased number of CFU-Mix, up-regulated in SIRT6 mRNA and protein expression, down-regulation in NF-KB mRNA and protein expression. The Rg1 anti-senescence group showed more evident changes in indexes than the Rg1-treated group.
  
  
  CONCLUSIONS
  Rg, may inhibit Sca-1 + HSC/HPC senescence induced by t-BHP by regulating SIRT6/NF-KB signal path.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yan-long
authors.initials: YL
authors.lastname: Tang
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Ji-chao
authors.initials: JC
authors.lastname: Ding
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D016922:Cellular Senescence / Q000187:drug effects*; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D037761:Sirtuins / Q000502:physiology*
chemical_list: D000950:Antigens, Ly; D036145:Ginsenosides; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D016328:NF-kappa B; C501860:Sirt6 protein, mouse; D037761:Sirtuins; C035054:ginsenoside Rg1