title: Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans.
abstract: The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organized into three hierarchical orders characterized by monomers, stable dimers formed by the occurrence of disulfide bonds, and hexamers resulting from a combination of dimers. The structural implications of these findings are discussed providing new elements for a more integrated model of this S-layer.
authors.affiliation: Laboratory of Plant Physiology and Photobiology, Department of Life and Environmental Sciences, University of Cagliari Cagliari, Italy.
authors.forename: Domenica
authors.initials: D
authors.lastname: Farci
authors.affiliation: Laboratory of Synchrotron Crystallography, Department of Structural Biology, European Molecular Biology Laboratory Grenoble, France ; Unit for Virus Host Cell Interactions, Laboratory of Structural Biology of RNA-Protein Complexes in Gene Expression and Host-Pathogen Interactions, University Grenoble Alpes-EMBL-Centre National de la Recherche Scientifique Grenoble, France.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Bowler
authors.affiliation: Laboratory of Molecular Virology, Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato Cagliari, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Esposito
authors.affiliation: Department of Photon Sciences, Brookhaven National Laboratory Upton, NY, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: McSweeney
authors.affiliation: Laboratory of Molecular Virology, Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato Cagliari, Italy.
authors.forename: Enzo
authors.initials: E
authors.lastname: Tramontano
authors.affiliation: Laboratory of Plant Physiology and Photobiology, Department of Life and Environmental Sciences, University of Cagliari Cagliari, Italy ; Laboratory of Structural Biology, International Institute of Molecular and Cell Biology Warsaw, Poland.
authors.forename: Dario
authors.initials: D
authors.lastname: Piano
keywords: DR_2577; Deinococcus radiodurans; S-layer; SlpA; hexagonally packed intermediate