title: Identification of novel long non-coding RNAs in triple-negative breast cancer.
abstract: Triple-negative breast carcinomas (TNBC) are characterized by particularly poor outcomes, and there are no established markers significantly associated with prognosis. Long non-coding RNAs (lncRNAs) are subclass of noncoding RNAs that have been recently shown to play critical roles in cancer biology. However, little is known about their mechanistic role in TNBC pathogenesis. In this report, we investigated the expression patterns of lncRNAs from TNBC tissues and matched normal tissues with Agilent Human lncRNA array. We identified 1,758 lncRNAs and 1,254 mRNAs that were differentially expressed (≥ 2-fold change), indicating that many lncRNAs are significantly upregulated or downregulated in TNBC. Among these, XR_250621.1 and NONHSAT125629 were the most upregulated and downregulated lncRNAs respectively. qRT-PCR was employed to validate the microarray analysis findings, and results were consistent with the data from the microarrays. GO and KEGG pathway analysis were applied to explore the potential lncRNAs functions, some pathways including microtubule motor activity and DNA replication were identified in TNBC pathogenesis. Our study revealed that a set of lncRNAs were differentially expressed in TNBC tissues, suggesting that they may play role in TNBC. These results shed light on lncRNAs' biological functions and provide useful information for exploring potential therapeutic targets for breast cancer.
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.|Institute of Cell Biology, Zhejiang University, Hangzhou, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Bojian
authors.initials: B
authors.lastname: Xie
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Internal Oncology, Hennan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Hennan, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.affiliation: Glycobiology and Glycochemistry Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Longyao
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Internal Oncology, Hennan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Hennan, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Glycobiology and Glycochemistry Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
authors.affiliation: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China.
authors.forename: Feilin
authors.initials: F
authors.lastname: Cao
mesh_terms: D014408:Biomarkers, Tumor; D016000:Cluster Analysis; D004261:DNA Replication; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D014018:Tissue Distribution; D064726:Triple Negative Breast Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: long non-coding RNAs; triple-negative breast carcinomas