title: An actin-myosin-II interaction is involved in maintaining the contractile ring in fission yeast.
abstract: The actomyosin-based contractile ring, which assembles at the cell equator, maintains its circularity during cytokinesis in many eukaryotic cells, ensuring its efficient constriction. Although consistent maintenance of the ring is one of the mechanisms underpinning cytokinesis, it has not yet been fully addressed. We here investigated the roles of fission yeast myosin-II proteins [Myo2 and Myo3 (also known as Myp2)] in ring maintenance during cytokinesis, with a focus on Myo3. A site-directed mutational analysis showed that the motor properties of Myo3 were involved in its accumulation in the contractile ring. The assembled ring was often deformed and not properly maintained under conditions in which the activities of myosin-II proteins localizing to the contractile ring were decreased, leading to inefficient cell division. Moreover, Myo3 appeared to form motile clusters on the ring. We propose that large assemblies of myosin-II proteins consolidate the contractile ring by continuously binding to F-actin in the ring, thereby contributing to its maintenance.
authors.affiliation: Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan masaktakaine@gmail.com knakano@biol.tsukuba.ac.jp.
authors.forename: Masak
authors.initials: M
authors.lastname: Takaine
authors.affiliation: Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Numata
authors.affiliation: Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan masaktakaine@gmail.com knakano@biol.tsukuba.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakano
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000185:Actinin / Q000235:genetics; D000199:Actins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D048749:Cytokinesis / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; C410737:Cdc8 protein, S pombe; D018797:Cell Cycle Proteins; C434211:MYO2 protein, S pombe; C110514:Myp2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D000185:Actinin; D024462:Myosin Type II; D018995:Myosin Heavy Chains
keywords: Actin cytoskeleton; Contractile ring; Cytokinesis; Fission yeast; Myosin-II