title: Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.
abstract: To adapt to an ever-changing environment, animals consolidate some, but not all, learning experiences to long-term memory. In mammals, long-term memory consolidation often involves neural pathway reactivation hours after memory acquisition. It is not known whether this delayed-reactivation schema is common across the animal kingdom or how information is stored during the delay period. Here, we show that, during courtship suppression learning, Drosophila exhibits delayed long-term memory consolidation. We also show that the same class of dopaminergic neurons engaged earlier in memory acquisition is also both necessary and sufficient for delayed long-term memory consolidation. Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation. Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII. Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.
authors.affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Krüttner
authors.affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056 Basel, Switzerland.
authors.forename: Lisa
authors.initials: L
authors.lastname: Traunmüller
authors.affiliation: Janelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Ugur
authors.initials: U
authors.lastname: Dag
authors.affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Jandrasits
authors.affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; The Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Stepien
authors.affiliation: Janelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Nirmala
authors.initials: N
authors.lastname: Iyer
authors.affiliation: Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RA Leiden, the Netherlands.
authors.forename: Lee G
authors.initials: LG
authors.lastname: Fradkin
authors.affiliation: Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RA Leiden, the Netherlands.
authors.forename: Jasprina N
authors.initials: JN
authors.lastname: Noordermeer
authors.affiliation: Janelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Brett D
authors.initials: BD
authors.lastname: Mensh
authors.affiliation: Janelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA; Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria. Electronic address: kelemank@janelia.hhmi.org.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Keleman
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics*; D007858:Learning / Q000502:physiology; D000069077:Memory Consolidation / Q000502:physiology*; D057567:Memory, Long-Term / Q000502:physiology*; D024521:Mushroom Bodies / Q000502:physiology; D009474:Neurons / Q000502:physiology; D013569:Synapses / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; C529066:Orb2 protein, Drosophila; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2