title: Newly identified protein Imi1 affects mitochondrial integrity and glutathione homeostasis in Saccharomyces cerevisiae.
abstract: Glutathione homeostasis is crucial for cell functioning. We describe a novel Imi1 protein of Saccharomyces cerevisiae affecting mitochondrial integrity and involved in controlling glutathione level. Imi1 is cytoplasmic and, except for its N-terminal Flo11 domain, has a distinct solenoid structure. A lack of Imi1 leads to mitochondrial lesions comprising aberrant morphology of cristae and multifarious mtDNA rearrangements and impaired respiration. The mitochondrial malfunctioning is coupled to significantly decrease the level of intracellular reduced glutathione without affecting oxidized glutathione, which decreases the reduced/oxidized glutathione ratio. These defects are accompanied by decreased cadmium sensitivity and increased phytochelatin-2 level.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kowalec
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Grynberg
authors.affiliation: Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), 02-776 Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Pająk
authors.affiliation: Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Socha
authors.affiliation: Department of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Winiarska
authors.affiliation: Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Fronk
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland ania218@ibb.waw.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Kurlandzka
mesh_terms: D002104:Cadmium / Q000633:toxicity; D004734:Energy Metabolism; D005978:Glutathione / Q000378:metabolism*; D006706:Homeostasis; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D054811:Phytochelatins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C000606934:Imi1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D002104:Cadmium; D054811:Phytochelatins; D005978:Glutathione
keywords: IMI1 gene; glutathione; mitochondria; phytochelatin; yeast