title: [Influence of stir-baked with sand on active ingredients, diarrhea and hepatoprotection of Herpetospermum caudigerum].
abstract: To study the influence of stir-baked with sand on active ingredients, diarrhea and hepatoprotection of Herpetospermum caudigerum, the contents of herperione and herpetin in H. caudigerum before and after stir-baking with sand were analyzed by HPLC. The effect of stir-baked with sand on diarrhea of H. caudigerum TL was evaluated using the mean stool rate (MSR) and mean diarrheal index ( MDI) and the influence of stir-baked with sand on hepatoprotective effect of H. caudigerum TL was examined using a mouse model of CCl4-induced liver injury based on the analysis of serum ALT and AST activities. The results of HPLC analysis showed the content of herperione in H. caudigerum after stir-baking with sand decreased by 40.9% (P < 0.01) and the content of herpetin had no change. Pharmacodynamic results showed that the MSR and MDI of high-dose and middle-dose group of H. caudigerum TL after stir-baking with sand were significantly lower than that of high-dose and middle-dose group of H. caudigerum TL without stir-baking with sand; The high-dose and middle-dose of H. caudigerum TL with/without stir-baking with sand significantly alleviated liver injury as indicated by the decreased levels of serum ALT and AST, but the ALT and AST levels of high-dose and middle-dose group of H. caudigerum TL after stir-baking with sand were higher than that of H. caudigerum TL without stir-baking with sand. The results revealed that the stir-baking with sand could effectively relieve diarrhea effect of H. caudigerum TL, while it also reduces the hepatoprotection of H. caudigerum TL.
authors.forename: Juan-juan
authors.initials: JJ
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.forename: Rong-li
authors.initials: RL
authors.lastname: Yin
authors.forename: Cheng-ying
authors.initials: CY
authors.lastname: Shen
authors.forename: Ling
authors.initials: L
authors.lastname: Cheng
authors.forename: Ling
authors.initials: L
authors.lastname: Qiu
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003296:Cooking; D019662:Cucurbitaceae / Q000737:chemistry*; D003967:Diarrhea / Q000139:chemically induced*; D005260:Female; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology
chemical_list: D020011:Protective Agents