title: c-Myb is required for plasma cell migration to bone marrow after immunization or infection.
abstract: Plasma cell migration is crucial to immunity, but little is known about the molecular regulators of their migratory programs. Here, we detail the critical role of the transcription factor c-Myb in determining plasma cell location. In the absence of c-Myb, no IgG(+) antigen-specific plasma cells were detected in the bone marrow after immunization or virus infection. This was correlated with a dramatic reduction of plasma cells in peripheral blood, mislocalization in spleen, and an inability of c-Myb-deficient plasma cells to migrate along a CXCL12 gradient. Therefore, c-Myb plays an essential, novel role in establishing the long-lived plasma cell population in the BM via responsiveness to chemokine migration cues.
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia jacobson@wehi.edu.au tarlinton@wehi.edu.au.
authors.forename: Kim L
authors.initials: KL
authors.lastname: Good-Jacobson
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
authors.forename: Kristy
authors.initials: K
authors.lastname: O'Donnell
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Gabrielle T
authors.initials: GT
authors.lastname: Belz
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Nutt
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia jacobson@wehi.edu.au tarlinton@wehi.edu.au.
authors.forename: David M
authors.initials: DM
authors.lastname: Tarlinton
mesh_terms: D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D058501:Enzyme-Linked Immunospot Assay; D005434:Flow Cytometry; D006652:Histological Techniques; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010950:Plasma Cells / Q000378:metabolism / Q000502:physiology*; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D017931:DNA Primers; D007074:Immunoglobulin G; D020598:Proto-Oncogene Proteins c-myb