title: Understanding the plant-pathogen interactions in the context of proteomics-generated apoplastic proteins inventory.
abstract: The extracellular space between cell wall and plasma membrane acts as the first battle field between plants and pathogens. Bacteria, fungi, and oomycetes that colonize the living plant tissues are encased in this narrow region in the initial step of infection. Therefore, the apoplastic region is believed to be an interface which mediates the first crosstalk between host and pathogen. The secreted proteins and other metabolites, derived from both host and pathogen, interact in this apoplastic region and govern the final relationship between them. Hence, investigation of protein secretion and apoplastic interaction could provide a better understanding of plant-microbe interaction. Here, we are briefly discussing the methods available for the isolation and normalization of the apoplastic proteins, as well as the current state of secretome studies focused on the in-planta interaction between the host and the pathogen.
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
authors.forename: So Eui
authors.initials: SE
authors.lastname: Lee
authors.affiliation: Research Laboratory for Biotechnology and Biochemistry Kathmandu, Nepal ; Global Research Arch for Developing Education (GRADE), Academy Private Limited Birgunj, Nepal.
authors.forename: Ganesh K
authors.initials: GK
authors.lastname: Agrawal
authors.affiliation: Research Laboratory for Biotechnology and Biochemistry Kathmandu, Nepal ; Global Research Arch for Developing Education (GRADE), Academy Private Limited Birgunj, Nepal ; Organization for Educational Initiatives, University of Tsukuba Tsukuba, Japan ; Faculty of Health and Sport Sciences, Tsukuba International Academy for Sport Studies, University of Tsukuba Tsukuba, Japan.
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.affiliation: Bio-crop Development Division, National Academy of Agricultural Science, Rural Development Administration Jeonju, South Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research Cologne, Germany.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
authors.forename: Sun T
authors.initials: ST
authors.lastname: Kim
keywords: apoplast; apoplastic proteins; effector-triggered immunity; pattern-triggered immunity; plant-pathogen interaction; protein secretion; secretome