title: Characterization of the anaerobic denitrification bacterium Acinetobacter sp. SZ28 and its application for groundwater treatment.
abstract: Acinetobacter sp. SZ28 exhibited efficient autotrophic denitrification ability using Mn(2+) as an electron donor. Sequence amplification identified the presence of the nirS gene. Meteorological chromatography analysis showed that N2 was produced as an end product. Response surface methodology experiments showed that the maximum removal of nitrate occurred under the following conditions: Mn(2+) concentration of 143.56 mg/L, C/N ratio of 6.82, initial pH of 5.17, and temperature of 34.26 °C, where the initial Mn(2+) concentration produced the largest effect. In the groundwater experiment, nitrate levels decreased from 1.63 mg/L to 0 mg/L. Three-dimensional fluorescence analysis showed a decrease in the peak intensity of the original humus. Humus and the small-molecule amino acid tryptophan were detected. These results demonstrated that strain SZ28 is a suitable candidate for the simultaneous removal of nitrogen and Mn(2+) in groundwater treatment.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Jun feng
authors.initials: Jf
authors.lastname: Su
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Sheng Chen
authors.initials: SC
authors.lastname: Zheng
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Ting lin
authors.initials: Tl
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: mafang@hit.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Si Cheng
authors.initials: SC
authors.lastname: Shao
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Shao Fei
authors.initials: SF
authors.lastname: Yang
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Li na
authors.initials: Ln
authors.lastname: Zhang
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000378:metabolism*; D000693:Anaerobiosis / Q000502:physiology; D001673:Biodegradation, Environmental; D003198:Computer Simulation; D060587:Groundwater / Q000382:microbiology*; D008345:Manganese / Q000302:isolation & purification / Q000378:metabolism*; D008954:Models, Biological; D009566:Nitrates / Q000302:isolation & purification / Q000378:metabolism*; D058465:Nitrification; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D009566:Nitrates; D014874:Water Pollutants, Chemical; D008345:Manganese
keywords: Acinetobacter sp.; Anaerobic Mn(2+) oxidizing denitrifier (AMOD); Nitrate removal; Optimization