title: Evaluation of blood, buccal swabs, and hair follicles for DNA profiling technique using STR markers.
abstract: OBJECTIVE
  To study the short tandem repeat (STR) pattern of DNA from the blood, buccal swabs, and hair follicles of the recipients of allogenic hematopoietic stem cell transplantation to examine whether these tissues contain donor derived cells.
  
  
  METHODS
  The study enrolled 25 patients who sustained engraftment. Peripheral blood, buccal swabs, and hair follicles were collected on days 21-30, 90, and 180 after transplantation and the chimeric status of the recipients was evaluated.
  
  
  RESULTS
  Donor derived cells existed in the blood and buccal swabs, but not in hair follicles, which can be used to obtain the pre-transplant sample of the recipient after transplant.
  
  
  CONCLUSIONS
  Peripheral blood and buccal swab do not serve as a reliable source of recipient's origin for DNA analysis of individuals who underwent allogenic hematopoietic stem cell transplantation at least within 6 months after transplant.
authors.forename: Garima
authors.initials: G
authors.lastname: Chaudhary
authors.forename: T D
authors.initials: TD
authors.lastname: Dogra
authors.affiliation: Anupuma Raina, Department of Forensic Medicine, AIIMS, New Delhi, India, anupumaraina@gmail.com.
authors.forename: Anupuma
authors.initials: A
authors.lastname: Raina
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D003584:Cytological Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D018859:Hair Follicle / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology*; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D015415:Biomarkers