title: Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation.
abstract: This study was undertaken to examine the effect of cholesterol-loaded-cyclodextrin (CLC) on boar sperm viability and spermatozoa cryosurvival during boar semen cryopreservation, and methyl-β-cyclodextrin (MBCD) was treated for comparing with CLC. Boar semen treated with CLC and MBCD before freezing process to monitor the effect on survival and capacitation status by flow cytometry with appropriate fluorescent probes. Sperm viability was higher in 1.5mg CLC-treated sperm (76.9±1.01%, P<0.05) than un-treated and MBCD-treated sperm before cryopreservation (58.7±1.31% and 60.3±0.31%, respectively). For CTC patterns, F-pattern was higher in CLC treated sperm than MBCD-treated sperm, for B-pattern was higher in CLC-treated sperm than fresh sperm (P<0.05). For AR pattern (an acrosome-reacted sperm) was lower in CLC-treated sperm than MBCD-treated sperm (P<0.05). Moreover, we examined in vitro development of porcine oocytes after in vitro fertilization using CLC-treated frozen-thawed semen, in which CLC treatment prior to freezing and thawing increased the development of oocytes to blastocyst stage in vitro. In conclusion, CLC could protect the viability of spermatozoa from cryodamage prior to cryopreservation in boar semen.
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Yong-Seung
authors.initials: YS
authors.lastname: Lee
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea; Institute of Animal Resources, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: shung0626@gmail.com.
authors.forename: Seunghyung
authors.initials: S
authors.lastname: Lee
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Boo-Keun
authors.initials: BK
authors.lastname: Yang
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Choon-Keun
authors.initials: CK
authors.lastname: Park
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003505:Cyclodextrins / Q000494:pharmacology*; D008297:Male; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013552:Swine; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol
keywords: Cholesterol-loaded-cyclodextrin; Cryopreservation; Spermatozoa; Viability