title: [Evaluation on contribution rate of each component total salvianolic acids and characterization of apparent oil/water partition coefficient].
abstract: The difference between three representative components of total salvianolic acids in pharmacodynamic activity were compared by three different pharmacological experiments: HUVECs oxidative damage experiment, 4 items of blood coagulation in vitro experiment in rabbits and experimental myocardial ischemia in rats. And the effects of contribution rate of each component were calculated by multi index comprehensive evaluation method based on CRITIC weights. The contribution rates of salvianolic acid B, rosmarinic acid and Danshensu were 28.85%, 30.11%, 41.04%. Apparent oil/water partition coefficient of each representative components of total salvianolic acids in n-octyl alcohol-buffer was tested and the total salvianolic acid components were characterized based on a combination of the approach of self-defined weighting coefficient with effects of contribution rate. Apparent oil/water partition coefficient of total salvianolic acids was 0.32, 1.06, 0.89, 0.98, 0.90, 0.13, 0.02, 0.20, 0.56 when in octanol-water/pH 1.2 dilute hydrochloric acid solution/ pH 2.0, 2.5, 5.0, 5.8, 6.8, 7.4, 7.8 phosphate buffer solution. It provides a certain reference for the characterization of components.
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Yan
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Chen
authors.forename: Hai-jian
authors.initials: HJ
authors.lastname: Xia
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Jia
authors.forename: Zhen-hai
authors.initials: ZH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D002934:Cinnamates / Q000737:chemistry / Q000494:pharmacology*; D053630:Depsides / Q000737:chemistry / Q000494:pharmacology*; D007773:Lactates / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D000097123:Rosmarinic Acid
chemical_list: D001572:Benzofurans; D002934:Cinnamates; D053630:Depsides; D007773:Lactates; D000097123:Rosmarinic Acid; C035055:3,4-dihydroxyphenyllactic acid; C076944:salvianolic acid B