title: Genetic variability of DNA repair mechanisms influences chemotherapy outcome of gastric cancer.
abstract: Genetic variability of DNA repair mechanisms influences chemotherapy treatment outcome of gastric cancer. We conducted a cohort study to investigate the role of ERCC1-ERCC2 gene polymorphisms in the chemotherapy response and clinic outcome of gastric cancer. Between March 2011 and March 2013, 228 gastric patients who were newly diagnosed with histopathology were enrolled in our study. Genotypes of ERCC1 rs11615, rs3212986, rs2298881 and ERCC2 rs3212986 were conducted by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. We found that individuals carrying TT genotype of ERCC1 rs11615 and CC genotype of ERCC1 rs2298881 were associated with better response to chemotherapy and longer survival time of gastric cancer. Moreover, individuals with AA genotype of ERCC2 rs1799793 were correlated with shorter survival of gastric cancer. In conclusion, ERCC1 rs11615, rs2298881 and ERCC2 rs1799793 polymorphism play an important role in the treatment outcome of gastric cancer.
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University Guangzhou, China ; Department of General Surgery, Affilated Renmin Hospital of Inner Mongolia Medical University Huhhot, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Feature Section, The Second Affiliated Hospital of Inner Mongolia Medical University Huhhot, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Affilated Renmin Hospital of Inner Mongolia Medical University Huhhot, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Feature section, The Second Affiliated Hospital of Inner Mongolia Medical University Huhhot, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University Guangzhou, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Feature section, The Second Affiliated Hospital of Inner Mongolia Medical University Huhhot, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D014408:Biomarkers, Tumor / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D018450:Disease Progression; D004720:Endonucleases / Q000235:genetics*; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D053208:Kaplan-Meier Estimate; D002955:Leucovorin / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D009944:Organoplatinum Compounds / Q000627:therapeutic use; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D016016:Proportional Hazards Models; D012074:Remission Induction; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D013997:Time Factors; D016896:Treatment Outcome; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D009944:Organoplatinum Compounds; C071447:ERCC1 protein, human; D004720:Endonucleases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D002955:Leucovorin; D005472:Fluorouracil
keywords: ERCC1; ERCC2; gastric cancer; overall survival; polymorphism; response to chemotherapy