title: Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses.
abstract: Reactive oxygen species (ROS) are signaling molecules that regulate plant development and responses to stresses. Mitochondria are the source of most ROS in heterotrophic cells, and mitochondrial complex I and complex III are regarded as the main sites of ROS production in plant mitochondria. Recent studies have demonstrated that succinate dehydrogenase (SDH) also contributes to mitochondrial ROS production. However, the ability of SDH to generate ROS in plants is unclear. The aim of this study was to evaluate the role of SDH in mitochondrial ROS production. Our results demonstrated that SDH is a direct source of ROS in Arabidopsis thaliana and Oryza sativa, and the induction of ROS production by specific SDH inhibitors impaired plant growth. In addition, this effect was accompanied by the down-regulation of cell cycle genes and the up-regulation of stress-related genes. However, the partial inhibition of SDH by a competitive inhibitor decreased ROS production, which was associated with increased shoot and root growth, and prevented the down-regulation of cell cycle genes and the induction of stress-related genes by noncompetitive inhibitors. In conclusion, SDH plays an important role in ROS production, being a direct source of ROS in plant mitochondria and regulating plant development and stress responses.
authors.affiliation: Programa de Pós Graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Douglas
authors.initials: D
authors.lastname: Jardim-Messeder
authors.affiliation: Programa de Pós Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Andréia
authors.initials: A
authors.lastname: Caverzan
authors.affiliation: Programa de Pós Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Rauber
authors.affiliation: Programa de Pós Graduação em Bioquímica e Biologia Celular, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: de Souza Ferreira
authors.affiliation: Programa de Pós Graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.|Programa de Pós Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Margis-Pinheiro
authors.affiliation: Programa de Pós Graduação em Bioquímica e Biologia Celular, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Galina
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D002453:Cell Cycle; D008928:Mitochondria / Q000378:metabolism*; D063245:Plant Development; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D013385:Succinate Dehydrogenase
keywords: Arabidopsis thaliana; Oryza sativa; plant development; reactive oxygen species (ROS); succinate dehydrogenase (SDH)