title: Evaluation of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered Brassica juncea (Indian mustard) for potential risks of food allergy using bioinformatics and literature searches.
abstract: The potential allergenicity of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered mustard for pollination control in plant breeding was evaluated for regulatory review. To evaluate the potential allergenicity of the Bar, Barnase and Barstar proteins amino acid sequence comparisons were made to those of known and putative allergens, and search for published evidence to the sources of the genes using the AllergenOnline.org database. Initial comparisons in 2012 were performed with version 12 by methods recommended by the Codex Alimentarius Commission and the Indian Council of Medical Research, Government of India. Searches were repeated with version 15 in 2015. A literature search was performed using PubMed to identify reports of allergy associated with the sources of the three transgenes. Potential open reading frames at the DNA insertion site were evaluated for matches to allergens. No significant sequence identity matches were identified with Bar, Barnase or Barstar proteins or potential fusion peptides at the genomic-insert junctions compared to known allergens. No references were identified that associated the sources of the genes with allergy. Based on these results we conclude that the Bar, Barnase and Barstar proteins are unlikely to present any significant risk of food allergy to consumers.
authors.affiliation: Food & Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India. Electronic address: vasanthi.siruguri@gmail.com.
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Siruguri
authors.affiliation: Food & Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India. Electronic address: nindineshpct@gmail.com.
authors.forename: Dinesh Kumar
authors.initials: DK
authors.lastname: Bharatraj
authors.affiliation: Biomedical Informatics Centre(BIC), National Institute of Nutrition(NIN), Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India. Electronic address: vrajunaik@live.com.
authors.forename: Raju Naik
authors.initials: RN
authors.lastname: Vankudavath
authors.affiliation: Biomedical Informatics Centre(BIC), National Institute of Nutrition(NIN), Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India; Division of Biostatistics, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India. Electronic address: dr_vishnurao@yahoo.com.
authors.forename: Vishnu Vardhana Rao
authors.initials: VV
authors.lastname: Mendu
authors.affiliation: Centre for Genetic Manipulation of Crop Plants, University of Dehli South Campus, New Delhi 110021, India. Electronic address: vibha.mustard@gmail.com.
authors.forename: Vibha
authors.initials: V
authors.lastname: Gupta
authors.affiliation: Food Allergy Research and Resource Program, Dept. of Food Science & Technology, University of Nebraska-Lincoln, Lincoln 68583-0955, NE, USA. Electronic address: rgoodman2@unl.edu.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Goodman
mesh_terms: D000123:Acetyltransferases / Q000009:adverse effects / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000485:Allergens / Q000009:adverse effects* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000009:adverse effects / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000009:adverse effects* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D030562:Databases, Protein; D004044:Dietary Proteins / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism; D005512:Food Hypersensitivity / Q000209:etiology / Q000517:prevention & control; D030841:Food, Genetically Modified / Q000009:adverse effects*; D006801:Humans; D007194:India; D008958:Models, Molecular; D009149:Mustard Plant / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D054817:Pollination; D011994:Recombinant Proteins / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000009:adverse effects / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018570:Risk Assessment; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D001426:Bacterial Proteins; D004044:Dietary Proteins; D011994:Recombinant Proteins; C057438:barstar protein, Bacillus amyloliquefaciens; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease
keywords: Allergenicity; Bar; Barnase; Barstar; Bioinformatics; GM crops