title: Plasmonic Tipless Pyramid Arrays for Cell Poration.
abstract: Improving the efficiency, cell survival, and throughput of methods to modify and control the genetic expression of cells is of great benefit to biology and medicine. We investigate, both computationally and experimentally, a nanostructured substrate made of tipless pyramids for plasmonic-induced transfection. By optimizing the geometrical parameters for an excitation wavelength of 800 nm, we demonstrate a 100-fold intensity enhancement of the electric near field at the cell-substrate contact area, while the low absorption typical for gold is maintained. We demonstrate that such a substrate can induce transient poration of cells by a purely optically induced process.
authors.affiliation: †Group of Applied Physics, Biophotonics Group, University of Geneva, 22 chemin de Pinchat, 1227 Carouge, Switzerland.|‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Courvoisier
authors.affiliation: ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Nabiha
authors.initials: N
authors.lastname: Saklayen
authors.affiliation: ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Marinus
authors.initials: M
authors.lastname: Huber
authors.affiliation: ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.|⊥School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.|§Department of Electrical Engineering, California NanoSystems Institute, UCLA, Los Angeles, California 90095, United States.
authors.forename: Eric D
authors.initials: ED
authors.lastname: Diebold
authors.affiliation: †Group of Applied Physics, Biophotonics Group, University of Geneva, 22 chemin de Pinchat, 1227 Carouge, Switzerland.
authors.forename: Luigi
authors.initials: L
authors.lastname: Bonacina
authors.affiliation: †Group of Applied Physics, Biophotonics Group, University of Geneva, 22 chemin de Pinchat, 1227 Carouge, Switzerland.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Wolf
authors.affiliation: ‡Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Mazur
mesh_terms: D002470:Cell Survival; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D055095:Optics and Photonics; D020349:Surface Plasmon Resonance; D014162:Transfection / Q000379:methods*
chemical_list: D006046:Gold
keywords: Cell transfection; nanophotonics; nanostructured substrate; plasmonics; tipless pyramid; ultrafast laser