title: Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis.
abstract: The aim of this work was to compare the effects of electrosynthesis on different bacterial species. The effects of neutral red-mediated electrosynthesis on the metabolite profiles of three microorganisms: Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis, were measured and compared and contrasted. A statistically comprehensive analysis of neutral red-mediated electrosynthesis is presented using the analysis of end-product profiles, current delivered, and changes in cellular protein expression. K. pneumoniae displayed the most dramatic response to electrosynthesis of the three bacteria, producing 93% more ethanol and 76% more lactate vs. control fermentation with no neutral red and no electron delivery. Z. mobilis showed no response to electrosynthesis except elevated acetate titers. Stoichiometric comparison showed that NAD(+) reduction by neutral red could not account for changes in metabolites during electrosynthesis. Neutral red-mediated electrosynthesis was shown to have multifarious effects on the three species.
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Harrington
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Abdelrhman
authors.initials: A
authors.lastname: Mohamed
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Vi N
authors.initials: VN
authors.lastname: Tran
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Saeid
authors.initials: S
authors.lastname: Biria
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Gargouri
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.
authors.forename: Jeong-Jin
authors.initials: JJ
authors.lastname: Park
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Gang
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA. Electronic address: beyenal@wsu.edu.
authors.forename: Haluk
authors.initials: H
authors.lastname: Beyenal
mesh_terms: D061249:Batch Cell Culture Techniques; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D055664:Electrochemical Techniques; D004583:Electrons; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D009499:Neutral Red / Q000378:metabolism*; D017355:Zymomonas / Q000254:growth & development / Q000378:metabolism*
chemical_list: D009499:Neutral Red
keywords: Bioelectrochemical system; Electron transfer; Electrosynthesis; Neutral red