title: Collagen esterification enhances the function and survival of pancreatic β cells in 2D and 3D culture systems.
abstract: Collagen, one of the most important components of the extracellular matrix (ECM), may play a role in the survival of pancreatic islet cells. In addition, chemical modifications that change the collagen charge profile to a net positive charge by esterification have been shown to increase the adhesion and proliferation of various cell types. The purpose of this study was to characterize and compare the effects of native collagen (NC) and esterified collagen (EC) on β cell function and survival. After isolation by the collagenase digestion technique, rat islets were cultured with NC and EC in 2 dimensional (2D) and 3 dimensional (3D) environments for a long-term duration in vitro. The cells were assessed for islet adhesion, morphology, viability, glucose-induced insulin secretion, and mRNA expression of glucose metabolism-related genes, and visualized by scanning electron microscopy (SEM). Islet cells attached tightly in the NC group, but islet cell viability was similar in both the NC and EC groups. Glucose-stimulated insulin secretion was higher in the EC group than in the NC group in both 2D and 3D culture. Furthermore, the mRNA expression levels of glucokinase in the EC group were higher than those in the NC group and were associated with glucose metabolism and insulin secretion. Finally, SEM observation confirmed that islets had more intact component cells on EC sponges than on NC sponges. These results indicate that modification of collagen may offer opportunities to improve function and viability of islet cells.
authors.affiliation: Regenerative Medicine Research Center, Dalim Tissen Co., LTD., 383-93, Yonnam-Dong, Mapo-gu, Seoul, South Korea.
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Ko
authors.affiliation: Regenerative Medicine Research Center, Dalim Tissen Co., LTD., 383-93, Yonnam-Dong, Mapo-gu, Seoul, South Korea; Asan Institute for Life Science, 388-1 Pungnap-2 Dong, Songpa-gu, Seoul, South Korea.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Asan Institute for Life Science, 388-1 Pungnap-2 Dong, Songpa-gu, Seoul, South Korea.
authors.forename: Seong Hee
authors.initials: SH
authors.lastname: Jeong
authors.affiliation: Asan Institute for Life Science, 388-1 Pungnap-2 Dong, Songpa-gu, Seoul, South Korea.
authors.forename: Song
authors.initials: S
authors.lastname: Lee
authors.affiliation: Regenerative Medicine Research Center, Dalim Tissen Co., LTD., 383-93, Yonnam-Dong, Mapo-gu, Seoul, South Korea.
authors.forename: Si-Nae
authors.initials: SN
authors.lastname: Park
authors.affiliation: Asan Institute for Life Science, 388-1 Pungnap-2 Dong, Songpa-gu, Seoul, South Korea. Electronic address: shimiink@gmail.com.
authors.forename: In Kyong
authors.initials: IK
authors.lastname: Shim
authors.affiliation: Asan Institute for Life Science, 388-1 Pungnap-2 Dong, Songpa-gu, Seoul, South Korea; Department of Surgery, University of Ulsan College of Medicine & Asan Medical Center, 388-1 Pungnap-2 Dong, Songpa-gu, Seoul, South Korea. Electronic address: drksc@amc.seoul.kr.
authors.forename: Song Cheol
authors.initials: SC
authors.lastname: Kim
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002470:Cell Survival; D003094:Collagen / Q000378:metabolism*; D004951:Esterification; D015870:Gene Expression; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D003094:Collagen
keywords: Diabetes; Esterified collagen; Extracellular matrix; Islet