title: Anti-neoplastic activities of Sepia officinalis ink and Coelatura aegyptiaca extracts against Ehrlich ascites carcinoma in Swiss albino mice.
abstract: OBJECTIVE
  With the development of sophisticated instruments for the isolation and elucidation of natural products structures from marine and freshwater organisms, major advances have been made in the discovery of aquatic derived therapeutics. Present investigations were carried out to evaluate cuttlefish (Sepia officinalis) ink extract (IE) and freshwater clam (Coelatura aegyptiaca) extract (CE) for their anticancer and antioxidant activities as compared to 5-flurouracil (5-Fu), in Ehrlich ascites carcinoma (EAC).
  
  
  METHODS
  Sixty female Swiss albino mice were divided into five groups (n = 12). All groups except group I received EAC cells (5 × 10(6) cells/mouse i.p.) and this was taken as the 0th day. Group I served as saline control (5 ml/kg 0.9% NaCl w/v p.o). Group II served as EAC control. Rats of groups III, IV and V received IE, CE (200 mg/kg body weight i.p.), and reference drug (5-Fu, 20 mg/kg body weight i.p.), respectively.
  
  
  RESULTS
  The reduction in tumor volume, packed cell volume, tumor cell counts and increase in median survival time and percentage increase in life span in treated animals were observed. There was a significant increase in RBC count; Hb content in treated animals and reduction in total WBC count. There was a significant decrease in AST, ALT, ALP and liver MDA levels and increase in GSH, SOD and NO levels were observed in all treated animals.
  
  
  CONCLUSIONS
  Both IE and CE were effective in inhibiting the tumor growth in ascitic tumor models. The biochemical, antioxidants and histopathological studies were also supported their antitumor properties.
authors.affiliation: Zoology Department, Faculty of Science, Cairo University Egypt.
authors.forename: Amel M
authors.initials: AM
authors.lastname: Soliman
authors.affiliation: Zoology Department, Faculty of Science, Cairo University Egypt.
authors.forename: Sohair R
authors.initials: SR
authors.lastname: Fahmy
authors.affiliation: Faculty of Science, El-Markb University El Khoms, Libya.
authors.forename: Salma A
authors.initials: SA
authors.lastname: El-Abied
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D049872:Bivalvia / Q000737:chemistry*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy*; D005260:Female; D006801:Humans; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D049833:Sepia / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001688:Biological Products; D015415:Biomarkers
keywords: Ehrlich acsites carcinoma; Sepia officinalis; antitumor; coelatura aegyptiaca; oxidative stress