title: Comparison between self-assembling peptide nanofiber scaffold (SAPNS) and fibrin sealant in neurosurgical hemostasis.
abstract: RADA16-I is a synthetic type I self-assembling peptide nanofiber scaffold (SAPNS) which may serve as a novel biocompatible hemostatic agent. Its application in neurosurgical hemostasis, however, has not been explored. Although RADA16-I is nontoxic and nonimmunogenic, its intrinsic acidity may potentially provoke inflammation in the surgically injured brain. We conducted an animal study to compare RADA16-I with fibrin sealant, a commonly used agent, with the hypothesis that the former would be a comparable alternative. Using a standardized surgical brain injury model, 30 Sprague-Dawley rats were randomized into three treatment groups: RADA16-I, fibrin sealant or gelatin sponge (control). Animals were sacrificed on day 3 and 42. Astrocytic and microglial infiltrations within the cerebral parenchyma adjacent to the operative site were significantly lower in the RADA16-I and fibrin sealant groups than control. RADA16-I did not cause more cellular inflammatory response despite its acidity when compared with fibrin sealant. Immunohistochemical studies showed infiltration by astrocytes and microglia into the fibrin sealant and RADA16-I grafts, suggesting their potential uses as tissue scaffolds. RADA16-I is a promising candidate for further translational and clinical studies that focus on its applications as a safe and effective hemostat, proregenerative nanofiber scaffold as well as drug and cell carrier.
authors.affiliation: Department of Neurosurgery, Peking University First Hospital, Beijing, China.|Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Fei-Fan
authors.initials: FF
authors.lastname: Xu
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.|Department of Physiology, Medical College of Jinan University, Guangzhou, China.
authors.forename: Yue-Chun
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Stella
authors.initials: S
authors.lastname: Sun
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Amy S W
authors.initials: AS
authors.lastname: Ho
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Derek
authors.initials: D
authors.lastname: Lee
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Karrie M Y
authors.initials: KM
authors.lastname: Kiang
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Wai-Man
authors.initials: WM
authors.lastname: Lui
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|Neurotrauma Laboratory, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Bai-Yun
authors.initials: BY
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: Wu-Tian
authors.initials: WT
authors.lastname: Wu
authors.affiliation: Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Gilberto K K
authors.initials: GK
authors.lastname: Leung
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001930:Brain Injuries / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D006488:Hemostasis, Surgical / Q000009:adverse effects / Q000379:methods*; D006490:Hemostatics / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D057139:Nanofibers; D019635:Neurosurgical Procedures; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D010455:Peptides; C546681:RADA16-I
keywords: RADA16-I; SAPNS; fibrin sealant; hemostasis; inflammation; nanomaterial; surgical brain injury