title: Minor and potentially toxic trace elements in milk and blood serum of dairy donkeys.
abstract: The aim of this trial was to study the concentration of Ti, V, As, Rb, Sr, Mo, Cd, Cs, and Pb in donkey milk and blood serum. One hundred twelve individual milk and blood serum samples were collected from 16 lactating donkeys (Martina-Franca-derived population; 6 to 12 yr old; 3 to 7 parities; average live weight 205.4kg; 32 to 58 d after foaling at the beginning of the trial) during a 3-mo-long experiment. The samples were analyzed for the aforementioned elements by inductively coupled plasma-mass spectrometry. Feedstuff and drinking water were also analyzed for the investigated elements. Data were processed by ANOVA for repeated measures. Average milk concentrations (±SD) of Ti, Rb, Sr, Mo, Cs, and Pb were 77.3 (±7.7), 339.1 (±82.1), 881.7 (±270.4), 4.5 (±1.6), 0.49 (±0.09), and 3.2 (±2.7) μg/L, respectively. More than 80% of samples were below the limit of detection for V, As, and Cd in milk and for Cd, and Pb in blood serum. The lower bound calculated for milk V, As, and Cd was 0.03μg/L for the 3 elements, the upper bound was calculated at 0.23, 0.10, and 0.31μg/L and the maximum value was observed at 0.54, 0.15, and 0.51μg/L, respectively. The average milk concentrations of Ti, Rb, Sr, Mo, and Cs were 600, 458, 346, 16, and 294%, respectively, than those of blood serum. Yet, Cs concentrations were in the same order of magnitude in milk and serum. Moderate to strong positive and significant correlation coefficients were observed between milk and blood serum concentrations for Ti, Rb, Sr, and Cs. The effect of the stage of lactation was significant for all the investigated elements in milk and blood serum, but most of the elements showed only small changes or inconsistent trends, and only the concentrations of Rb and Sr showed decreasing trends both in milk and blood serum. The relationship between milk and blood serum element concentrations indicates that the mammary gland plays a role in determining the milk concentrations of Mo, Ti, Rb, Sr, Mo, and Cs. In the current experimental conditions, in agreement with the low levels in drinking water and feedstuff, donkey milk concentration of potentially toxic elements was very low and did not raise health concerns for human consumption.
authors.affiliation: Dipartimento di Bioscienze e Medicina Veterinaria, Università degli Studi di Camerino, 62032 Camerino, Italy. Electronic address: francesco.fantuz@unicam.it.
authors.forename: F
authors.initials: F
authors.lastname: Fantuz
authors.affiliation: Dipartimento di Scienze e Tecnologie (sezione chimica), Università degli Studi di Camerino, 62032 Camerino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Ferraro
authors.affiliation: Dipartimento di Bioscienze e Medicina Veterinaria, Università degli Studi di Camerino, 62032 Camerino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Todini
authors.affiliation: Dipartimento di Scienze e Tecnologie (sezione chimica), Università degli Studi di Camerino, 62032 Camerino, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Piloni
authors.affiliation: Dipartimento di Bioscienze e Medicina Veterinaria, Università degli Studi di Camerino, 62032 Camerino, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Mariani
authors.affiliation: Department of Food Technology, Technological Educational Institute of Thessaly, 43100 Karditsa, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Malissiova
authors.affiliation: Dipartimento di Agricoltura, Ambiente, Alimenti, Università degli Studi del Molise, 86100 Campobasso, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Salimei
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D018397:Equidae; D005260:Female; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry; D011897:Random Allocation; D014131:Trace Elements / Q000097:blood / Q000378:metabolism*
chemical_list: D014131:Trace Elements
keywords: dairy donkey; donkey milk; occasionally beneficial element; potentially toxic element