title: Polydatin: a new therapeutic agent against multiorgan dysfunction.
abstract: BACKGROUND
  Polydatin (PD), a monocrystalline and polyphenolic drug isolated from a traditional Chinese herb (Polygonum cuspidatum), is protective against mitochondrial dysfunction and has been approved for clinical trials in the treatment of shock. However, whether the administration of PD has a therapeutic effect on multiple-organ dysfunction syndrome (MODS) requires investigation.
  
  
  METHODS
  MODS was induced in Sprague-Dawley rats via hemorrhage and ligation and puncture of cecum-induced sepsis. The rats were divided into three groups as follows: MODS + PD, MODS + normal saline, and a control group (no treatment). Survival time, blood biochemical indexes, and histopathologic changes in various organs were evaluated; serum oxidative stress (advanced oxidative protein products [AOPPs]) and proinflammatory cytokines (tumor necrosis factor-α, interleukin 1β, and interleukin 6) were assayed using enzyme-linked immunosorbent assay. Apoptosis-related protein expression (B-cell lymphoma-2 [Bcl-2] and Bax) was assayed by immunohistochemical and Western blotting methods, whereas caspase-3 activity was assayed by spectrophotometry.
  
  
  RESULTS
  PD improved organ function, prolonged survival time, and reduced MODS incidence and serum levels of AOPPs and proinflammatory cytokines. It also decreased Bax levels and caspase-3 activity and increased Bcl-2 levels in the kidney and liver.
  
  
  CONCLUSIONS
  PD may serve as a potential therapeutic for MODS, as it suppresses oxidative stress, inhibits inflammatory response, attenuates apoptosis, and protects against mitochondrial dysfunction.
authors.affiliation: Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou, P.R. China; Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou, P.R. China; Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China; Department of Anesthesiology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, P.R. China.
authors.forename: Youguang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Cai
authors.affiliation: Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou, P.R. China. Electronic address: kszhao@ymail.com.
authors.forename: Ke-seng
authors.initials: KS
authors.lastname: Zhao
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D005260:Female; D005960:Glucosides / Q000627:therapeutic use*; D009102:Multiple Organ Failure / Q000188:drug therapy* / Q000276:immunology / Q000401:mortality; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000627:therapeutic use*
chemical_list: D016207:Cytokines; D005960:Glucosides; D019253:Proto-Oncogene Proteins c-bcl-2; D013267:Stilbenes; C505410:Casp3 protein, rat; D053148:Caspase 3; C058229:polydatin
keywords: Apoptosis; Inflammation; Multiorgan dysfunction syndrome; Oxidative stress; Polydatin