title: Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins.
abstract: There is still a considerable need for development of new tools and methods detecting specific viral proteins for the diagnosis and pathogenesis study of the Yellow fever virus (YFV). This study aimed to develop and characterize polyclonal peptide antisera for detection of YFV-C and -NS1 proteins. The antisera were used further to investigate NS1 protein expression during YFV infection in mammalian cells. YFV target proteins were detected by all antisera in western blot and immunofluorescence assays. No cross-reactivity was observed with Dengue virus, West Nile virus, Tick-borne encephalitis virus and Japanese encephalitis virus. Nuclear localization of the YFV-C protein was demonstrated for the first time. Experiments investigating NS1 expression suggested a potential use of the YFV-NS1 antisera for development of diagnostic approaches targeting the secreted form of the NS1 protein. The antisera described in this study offer new possibilities for use in YFV research and for the development of novel diagnostic tests.
authors.affiliation: Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; The National Institute of Public Health, Prague, Czech Republic; European Program for Public Health Microbiology (EUPHEM), ECDC, Stockholm, Sweden. Electronic address: nkstock2015@gmail.com.
authors.forename: N K
authors.initials: NK
authors.lastname: Stock
authors.affiliation: Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Escadafal
authors.affiliation: Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; Freie Universität, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Achazi
authors.affiliation: Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; Université Montpellier 2, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Cissé
authors.affiliation: Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Niedrig
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D036022:Capsid Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry / Q000821:virology; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D003955:Diagnostic Tests, Routine / Q000379:methods; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D012680:Sensitivity and Specificity; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D014773:Virology / Q000379:methods; D015005:Yellow fever virus / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C100315:NS1 protein, Flavivirus; D010455:Peptides; D017361:Viral Nonstructural Proteins
keywords: Capsid protein; NS1 protein; Peptide antisera; Yellow fever virus