title: Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours.
abstract: Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.
authors.affiliation: Department of Pathology, Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
authors.forename: Tan A
authors.initials: TA
authors.lastname: Ince
authors.affiliation: Department of Pathology, Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
authors.forename: Aurea D
authors.identifier: http://orcid.org/0000-0002-9153-7414
authors.initials: AD
authors.lastname: Sousa
authors.affiliation: Department of Pathology, Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Jones
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27514, USA.
authors.forename: J Chuck
authors.initials: JC
authors.lastname: Harrell
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Elin S
authors.initials: ES
authors.lastname: Agoston
authors.affiliation: Department of Systems Biology, MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Marit
authors.initials: M
authors.lastname: Krohn
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Selfors
authors.affiliation: Department of Bioinformatics and Computational Biology, MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Bioinformatics and Computational Biology, MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Bioinformatics and Computational Biology, MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Mao
authors.initials: M
authors.lastname: Yong
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Buchwald
authors.affiliation: Department of Pathology, Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Systems Biology, MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Hale
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Evan
authors.initials: E
authors.lastname: Cohick
authors.affiliation: Vincent Center for Reproductive Biology, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Sergent
authors.affiliation: Department of Pathology, Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
authors.forename: Abigail
authors.initials: A
authors.lastname: Witt
authors.affiliation: Department of Pathology, Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
authors.forename: Zhanna
authors.initials: Z
authors.lastname: Kozhekbaeva
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Sizhen
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Agoston T
authors.initials: AT
authors.lastname: Agoston
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Merritt
authors.affiliation: Vincent Center for Reproductive Biology, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Foster
authors.affiliation: Vincent Center for Reproductive Biology, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
authors.forename: Bo R
authors.initials: BR
authors.lastname: Rueda
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Crum
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Joan S
authors.initials: JS
authors.lastname: Brugge
authors.affiliation: Department of Systems Biology, MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Gordon B
authors.initials: GB
authors.lastname: Mills
mesh_terms: D002277:Carcinoma / Q000473:pathology*; D045744:Cell Line, Tumor; D002945:Cisplatin; D003470:Culture Media; D004354:Drug Screening Assays, Antitumor; D005260:Female; D020869:Gene Expression Profiling; D064593:Heterografts; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D017239:Paclitaxel; D010641:Phenotype
chemical_list: D003470:Culture Media; D017239:Paclitaxel; D002945:Cisplatin