title: Effect of Uncaria tomentosa extract on purinergic enzyme activities in lymphocytes of rats submitted to experimental adjuvant arthritis model.
abstract: BACKGROUND
  Considering that adjuvant arthritis is an experimental model of arthritis widely used for preclinical testing of numerous anti-arthritic agents, which were taken by a large number of patients worldwide, it is of great interest to investigate the therapeutic action of compounds with anti-inflammatory properties, such as Uncaria tomentosa extract. Moreover, there are no studies demonstrating the effect of U. tomentosa on the metabolism of adenine nucleotides published so far. Thus, the purpose of the present study is to investigate the effects of U. tomentosa extract on E-NTPDase and E-ADA activities in lymphocytes of Complete Freund's Adjuvant (CFA) arthritis induced rats.
  
  
  METHODS
  To evaluate the effect of U. tomentosa extract on the activity of E-NTPDase and ADA in lymphocytes, the rats were submitted to an experimental adjuvant arthritis model. Peripheral lymphocytes were isolated and E-NTPDase and E-ADA activities were determined. Data were analyzed by a one- or two-way ANOVA. Post hoc analyses were carried out by the Student-Newman-Keuls (SNK) Multiple Comparison Test.
  
  
  RESULTS
  E-NTPDase activity was increased in arthritic untreated. Arthritic rats which received U. tomentosa extract, presented similar results to the control group. However, results obtained for adenosine hydrolysis by E-ADA were not altered in arthritic rats. U. tomentosa extract did not alter E-NTPDase and E-ADA activity in healthy animals.
  
  
  CONCLUSIONS
  The present investigation supports the hypothesis that the increased E-NTPDase activity verified in arthritic rats might be an attempt to maintain basal levels of ATP and ADP in the extracellular medium, since the arthritis induction causes tissue damage and, consequently, large amounts of ATP are released into this milieu. Also, it highlights the possibility to use U. tomentosa extract as an adjuvant to treat arthritis.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. liviagelain@gmail.com.
authors.forename: Lívia G
authors.initials: LG
authors.lastname: Castilhos
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. joaofprezer@hotmail.com.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. joaofprezer@hotmail.com.
authors.forename: João F P
authors.initials: JF
authors.lastname: Rezer
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. jbruchel@yahoo.com.br.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. jbruchel@yahoo.com.br.
authors.forename: Jader B
authors.initials: JB
authors.lastname: Ruchel
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. thorstenbergml@gmail.com.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. thorstenbergml@gmail.com.
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Thorstenberg
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. jeandreaugusto@hotmail.com.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. jeandreaugusto@hotmail.com.
authors.forename: Jeandre A dos S
authors.initials: JA
authors.lastname: Jaques
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. jobizzi@yahoo.com.br.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. jobizzi@yahoo.com.br.
authors.forename: Josiane B
authors.initials: JB
authors.lastname: Schlemmer
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. ph_doleski@hotmail.com.
authors.forename: Pedro H
authors.initials: PH
authors.lastname: Doleski
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. mateus.rossato@gmail.com.
authors.forename: Mateus F
authors.initials: MF
authors.lastname: Rossato
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. mariane_arnoldi@yahoo.com.br.
authors.forename: Mariane A
authors.initials: MA
authors.lastname: da Silva
authors.affiliation: Departamento de Ciências Morfológicas, Instituto de Ciências Básicas da Saúde, UFRGS, Laboratório de Estudos Sobre as Alterações Celulares e Teciduais, Porto Alegre, RS, Brazil. eacasali@gmail.com.|Laboratório de Enzimologia Aplicada ao Sistema Purinérgico, Departamento de Bioquímica, Instituto deCiências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil. eacasali@gmail.com.
authors.forename: Emerson André
authors.initials: EA
authors.lastname: Casalli
authors.affiliation: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. ritieldc@gmail.com.
authors.forename: Ritiel Corrêa
authors.initials: RC
authors.lastname: da Cruz
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. ferreiraj99@gmail.com.
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
authors.affiliation: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. margathayde@gmail.com.
authors.forename: Margareth L
authors.initials: ML
authors.lastname: Athayde
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. jamilefabbrin@yahoo.com.br.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. jamilefabbrin@yahoo.com.br.
authors.forename: Jamile F
authors.initials: JF
authors.lastname: Gonçalves
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 - Sala 4102, 97105-900, Santa Maria, RS, Brazil. dbitencourtrosaleal@gmail.com.|Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil. dbitencourtrosaleal@gmail.com.
authors.forename: Daniela B R
authors.initials: DB
authors.lastname: Leal
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000201:enzymology; D020948:Cat's Claw / Q000737:chemistry*; D005620:Freund's Adjuvant; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D010936:Plant Extracts; D005620:Freund's Adjuvant