title: Determination of Ethyl Carbamate in Chinese Yellow Rice Wine by Diatomaceous Earth Extraction and GC/MS Method.
abstract: A sensitive and rapid analytical method based on alkaline diatomaceous earth extraction followed by GC/MS was developed for the quantitative determination of the toxic contaminant ethyl carbamate (EC) in yellow rice wines. The optimal extraction conditions were investigated. With the application of diatomaceous earth extraction, the damage of organic acids to the capillary column was greatly reduced. By using d5-EC as an internal standard for quantitative analysis of EC, the linearity of the calibration curves was good between 10 and 1000 ng/mL. The LOD and LOQ were 1.7 and 5.0 μg/kg, respectively. The spiked level of EC was 5.0-300 μg/kg, and the average recovery of the spikes was between 78.4 and 98.2%, with an RSD between 4.3 and 8.3%. Upon validation by five laboratories when spiked with 50, 100, and 300 μg/kg, the average respective recoveries were 102.9, 102.2, and 98.7% with a RSD between 0.7 and 8.1%. The validation results demonstrated that the method is fast, simple, selective, and suitable for the determination of EC in yellow rice wines.
authors.affiliation: Center for Disease Control and Prevention of Zhejiang Province, Hangzhou 310021, People's Republic of China.
authors.forename: Pinggu
authors.initials: P
authors.lastname: Wu
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
authors.forename: Xianghong
authors.initials: X
authors.lastname: Shen
authors.forename: Liyuan
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tan
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Bingjie
authors.initials: B
authors.lastname: Ma
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
mesh_terms: D007692:Diatomaceous Earth; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D014520:Urethane / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D014520:Urethane; D007692:Diatomaceous Earth