title: Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection.
abstract: Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Shengwei
authors.initials: S
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Sciences, Shihezi University, Shihezi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ni
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Wujiafu
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Ma
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Sheng
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Pengyan
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Life Sciences, Shihezi University, Shihezi, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Cao
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000998:Antiviral Agents / Q000378:metabolism*; D060467:Disease Resistance; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D016019:Survival Analysis; D013552:Swine
chemical_list: D000998:Antiviral Agents; D034741:RNA, Small Interfering
keywords: FMDV; RNAi; infectious disease; microbiology; transgenic pigs; viruses