title: Some hormone, cytokine and chemokine levels that change across lifespan vary by cognitive status in male Fischer 344 rats.
abstract: We trained and tested young (6-8months; n=13), middle-aged (12-14months; n=41), and aged (22-24months; n=24) male Fischer 344 rats in a rapid acquisition water maze task and then quantified 27 stress hormones, cytokines and chemokines in their serum, hippocampi and frontal cortices using bead assay kits and xMAP technology. Middle-aged and aged rats learned the location of the hidden platform over training trials more slowly than their young counterparts. After training, young rats outperformed middle-aged and aged rats on both immediate and 24h retention probe trials and about half of the middle-aged and aged (aging) rats exhibited impaired performances when tested on the retention probe trial 24h later. The concentrations of many serum, hippocampal and cortical analytes changed with age often in networks that may represent age-sensitive signaling pathways and the concentrations of some of these analytes correlated with water maze learning and/or memory scores. Serum GRO/KC and RANTES levels, hippocampal GM-CSF levels and cortical IL-9 and RANTES levels were significantly higher in rats categorized as memory-impaired versus elite agers based upon their 24h probe trial performances. Our data add to the emerging picture of how age-related changes in immune and neuroimmune system signaling impacts cognition.
authors.affiliation: National Institute of Mental Health, NIH, Bethesda, MD, USA; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
authors.forename: Rachel B
authors.initials: RB
authors.lastname: Scheinert
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
authors.forename: Aditya
authors.initials: A
authors.lastname: Asokan
authors.affiliation: Department of Neuroscience, University of Florida, Gainesville, FL, USA; McKnight Brain Institute, University of Florida, Gainesville, FL, USA.
authors.forename: Asha
authors.initials: A
authors.lastname: Rani
authors.affiliation: Department of Neuroscience, University of Florida, Gainesville, FL, USA; McKnight Brain Institute, University of Florida, Gainesville, FL, USA.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Neuroscience, University of Florida, Gainesville, FL, USA; McKnight Brain Institute, University of Florida, Gainesville, FL, USA. Electronic address: foster1@ufl.edu.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Foster
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA; Department of Neuroscience, University of Florida, Gainesville, FL, USA; McKnight Brain Institute, University of Florida, Gainesville, FL, USA. Electronic address: bormerod@bme.ufl.edu.
authors.forename: Brandi K
authors.initials: BK
authors.lastname: Ormerod
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000378:metabolism; D000367:Age Factors; D000375:Aging / Q000378:metabolism* / Q000523:psychology*; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D018925:Chemokines / Q000097:blood / Q000378:metabolism*; D003071:Cognition / Q000502:physiology*; D003345:Corticosterone / Q000097:blood / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D006728:Hormones / Q000097:blood / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000097:blood / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D065853:Spatial Learning / Q000502:physiology
chemical_list: D018925:Chemokines; D016207:Cytokines; D006728:Hormones; D000324:Adrenocorticotropic Hormone; D008550:Melatonin; D003345:Corticosterone
keywords: Aging; Chemokines; Cytokines; Hippocampus; Inflammation; Memory; Neuroinflammation; Water maze; xMAP technology