title: Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells.
abstract: We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P < 0.001); however, QJJ restored HepG2 cell survival (P < 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (all P < 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (all P < 0.05). Furthermore, high-dose QJJ reduced both TNF-α and IL-6 levels as compared to the IR group (all P < 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Xiao-Tian
authors.initials: XT
authors.lastname: Zhang
authors.affiliation: Jilin Yizheng Pharmaceutical Group Co., Ltd., Siping, Jilin 136001, China.
authors.forename: Chun-Jiang
authors.initials: CJ
authors.lastname: Yu
authors.affiliation: Jilin Yizheng Pharmaceutical Group Co., Ltd., Siping, Jilin 136001, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Liu
authors.affiliation: The Second Department of Cardiology, The people's Liberation Army No. 208 Hospital, Changchun, Jilin 130021, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Zhang
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Chen-Xue
authors.initials: CX
authors.lastname: Song
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Jing-Shu
authors.initials: JS
authors.lastname: Xie
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Sai
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Jing-Tong
authors.initials: JT
authors.lastname: Zheng
authors.affiliation: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang