title: Application of GC/MS-based metabonomic profiling in studying the therapeutic effects of Huangbai-Zhimu herb-pair (HZ) extract on streptozotocin-induced type 2 diabetes in mice.
abstract: A protocol for metabolic profiling of mice urine was developed based on gas chromatograph-mass spectrometer (GC-MS) to explore metabolic state directly. The intra-day, inter-day, repeatability, and stability RSD for most endogenous compounds were less than 3%. Type 2 diabetic mellitus (T2DM) mice model was induced by high calorie diet combined with streptozocin. Urine from the control, T2DM and Huangbai-Zhimu herb-pair (HZ) treatment mice were enrolled in the subsequent study to show the usefulness of the method. OPLS-DA scores plots demonstrate that the cluster of T2DM mice is separated from that of control mice, while HZ-T2DM mice are located close to control mice, indicating that metabolic profiles of these HZ-T2DM mice are placed toward those of control group. The results illustrate that HZ treatment could lower the level of d-glucose, hexadecanoic acid, octadecanoic acid, propanoic acid, 3-hydroxybutyric acid, and 2,3-dihydroxybutanoic acid in urine of DM mice, meanwhile the results show that HZ treatment could ameliorate T2DM symptoms by intervening the fatty acid metabolism, starch and sucrose metabolism, and glyoxylate and dicarboxylate metabolism. This preliminary application indicated that the method is suitable and reliable for urine metabolic profiling. This study might explain the metabolic effects of T2DM and the mechanisms of action of HZ against T2DM.
authors.affiliation: Department of Experimental Teaching, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Song
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Hongyue
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemistry & Chemical Engineering, Tianjin University of Technology, 391 Binshui West Street, Xiqing District, Tianjin 300384, People's Republic of China.
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China.
authors.forename: Huilun
authors.initials: H
authors.lastname: Chu
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China; Tianjin JF-Pharmaland Technology Development Co., Ltd., Tianjin, China. Electronic address: zhuangpengwei@163.com.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Zhuang
authors.affiliation: College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Nankai District, Tianjin 300193, People's Republic of China. Electronic address: 13820188818@163.com.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012503:Saponins / Q000494:pharmacology*; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D012503:Saponins; C528424:huangbai; C060637:zhi-mu saponin; D013311:Streptozocin
keywords: GC–MS; Huangbai–Zhimu herb-pair; Type 2 diabetes; Urine metabonomics