title: Mass Spectral Profile for Rapid Differentiating Beta-Lactams from Their Ring-Opened Impurities.
abstract: High performance liquid chromatography tandem mass spectrometry (HPLC MS) has been widely used for β-lactam antibiotics determination. However, its application to identify impurities of these frequently used drugs is not sufficient at present. In this job, characteristic profiles of the collision induced dissociation (CID) spectra of both β-lactams and ring-opened β-lactams were extracted from the MS data of six β-lactam antibiotics and their forty-five impurities, and were confirmed by the MS data reported in the literature. These characteristics have been successfully applied to rapid differentiation of β-lactam and ring-opened β-lactam impurities in cefixime, cefdinir, and cefaclor. However, these characteristic profiles can only be obtained under low activating voltage. They did not display in the high energy activated CID spectra. Diagnostic fragmentations for determining the localization of double bond and substituents on the thiazine ring and the side chain were also observed. In addition, several characteristic fragmentations are hopeful to be used to differentiate the configurations of C-2 on the thiazine ring of ring-opened impurities, which is generally disadvantageous of mass spectrometry. Taken together, forty-five impurities were identified from the capsules of cefixime, cefdinir, and cefaclor.
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 Zhongguancun Street, South Haidian District, Beijing 100081, China ; Neuroscience Research Institute, Health Science Center, Peking University, Beijing 100191, China.
authors.forename: Hecheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Huang
authors.affiliation: National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cao
authors.affiliation: Neuroscience Research Institute, Health Science Center, Peking University, Beijing 100191, China.
authors.forename: Dehua
authors.initials: D
authors.lastname: Chui
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 Zhongguancun Street, South Haidian District, Beijing 100081, China.
authors.forename: Shengyuan
authors.identifier: 0000-0002-0039-8325
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D002433:Cefaclor / Q000737:chemistry; D000077525:Cefdinir; D020682:Cefixime / Q000737:chemistry; D002511:Cephalosporins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D047090:beta-Lactams / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D047090:beta-Lactams; D002433:Cefaclor; D020682:Cefixime; D000077525:Cefdinir