title: The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio.
abstract: The chromatin remodeler CHD5 plays a critical role in tumor suppression and neurogenesis in mammals. CHD5 contributes to gene expression during neurogenesis, but there is still much to learn regarding how this class of remodelers contributes to differentiation and development. CHD5 remodelers are vertebrate-specific, raising the prospect that CHD5 plays one or more conserved roles in this phylum. Expression of chd5 in adult fish closely mirrors expression of CHD5 in adult mammals. Knockdown of Chd5 during embryogenesis suggests new roles for CHD5 remodelers based on resulting defects in craniofacial development including reduced head and eye size as well as reduced cartilage formation in the head. In addition, knockdown of Chd5 results in altered expression of neural markers in the developing brain and eye as well as a profound defect in differentiation of dopaminergic amacrine cells. Recombinant zebrafish Chd5 protein exhibits nucleosome remodeling activity in vitro, suggesting that it is the loss of this activity that contributes to the observed phenotypes. Our studies indicate that zebrafish is an appropriate model for functional characterization of CHD5 remodelers in vertebrates and highlight the potential of this model for generating novel insights into the role of this vital class of remodelers.
authors.affiliation: Department of Biochemistry and Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Brett
authors.initials: B
authors.lastname: Bishop
authors.affiliation: Department of Biochemistry and Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Kwok Ki
authors.initials: KK
authors.lastname: Ho
authors.affiliation: Department of Biochemistry and Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Kim
authors.initials: K
authors.lastname: Tyler
authors.affiliation: Department of Biochemistry and Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Amanda
authors.initials: A
authors.lastname: Smith
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Bonilla
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Yuk Fai
authors.initials: YF
authors.lastname: Leung
authors.affiliation: Department of Biochemistry and Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States. Electronic address: ogas@purdue.edu.
authors.forename: Joe
authors.initials: J
authors.lastname: Ogas
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002356:Cartilage / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D059290:Dopaminergic Neurons / Q000502:physiology; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics*; D005123:Eye / Q000196:embryology / Q000378:metabolism; D006257:Head / Q000196:embryology*; D055495:Neurogenesis / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D029961:Zebrafish Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; C000602250:CHD5 protein, zebrafish
keywords: ATP-dependent chromatin remodeling; CHD remodeler; Embryogenesis; Neural development