title: MYC overexpression correlates with MYC amplification or translocation, and is associated with poor prognosis in mantle cell lymphoma.
abstract: OBJECTIVE
  We aimed to investigate MYC expression and chromosomal aberration in mantle cell lymphoma (MCL), and the clinical significance of these factors.
  
  
  RESULTS
  Sixty-five patients with MCL, including 54 classic, nine blastoid and two pleomorphic variants, were enrolled. Expression of MYC, Ki67 and p53 was assessed by immunohistochemistry. MYC amplification or translocation was examined by fluorescence in-situ hybridization. MYC expression was higher in blastoid/pleomorphic MCL variants (mean, 19.0%) than in classic MCL (mean, 1.9%; P < 0.001). Expression of p53 and Ki67 was also significantly higher in these variants. MYC amplification was found in two of 53 cases tested, both of which were blastoid variants with high MYC expression (29.7% and 20.4%). MYC translocation was found in two of 52 cases tested, both of which were pleomorphic variants with remarkably high MYC expression (68.5% and 71.0%). High MYC or p53 expression was significantly associated with shortened overall survival and progression-free survival in univariable and multivariable analyses (all P < 0.05).
  
  
  CONCLUSIONS
  MYC overexpression is a negative predictor of MCL patient outcomes. MYC gene amplification or translocation might be related to the pathogenesis of MCL, particularly in blastoid/pleomorphic variants.
authors.affiliation: Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea.|Department of Pathology, College of Medicine, Seoul National University, Seoul, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Choe
authors.affiliation: Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea.|Department of Pathology, College of Medicine, Seoul National University, Seoul, Korea.
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Yun
authors.affiliation: Department of Pathology, College of Medicine, Seoul National University, Seoul, Korea.|Department of Pathology, Seoul National University Hospital, Seoul, Korea.
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Na
authors.affiliation: Department of Pathology, Asan Medical Centre, Seoul, Korea.
authors.forename: Jooryung
authors.initials: J
authors.lastname: Huh
authors.affiliation: Department of Pathology, Asan Medical Centre, Seoul, Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Shin
authors.affiliation: Department of Pathology, Inha University Hospital, Incheon, Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Paik
authors.affiliation: Department of Pathology, Seoul National University Boramae Hospital, Seoul, Korea.
authors.forename: Young A
authors.initials: YA
authors.lastname: Kim
authors.affiliation: Department of Pathology, Seoul National University Hospital, Seoul, Korea.
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Nam
authors.affiliation: Department of Pathology, Seoul National University Hospital, Seoul, Korea.
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Jeon
authors.affiliation: Department of Pathology, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
authors.forename: Gyeongsin
authors.initials: G
authors.lastname: Park
authors.affiliation: Department of Pathology, College of Medicine, Seoul National University, Seoul, Korea.|Department of Pathology, Seoul National University Boramae Hospital, Seoul, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018572:Disease-Free Survival; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D019394:Ki-67 Antigen / Q000378:metabolism; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D014178:Translocation, Genetic; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53
keywords: Ki67; MYC gene; MYC protein; amplification; mantle cell lymphoma; p53; prognosis; translocation