title: Flow cytometry used to identify histiocytic sarcoma: A case report.
abstract: Histiocytic sarcoma (HS) is an extremely rare but aggressive malignancy of hematopoietic origin. The diagnosis of HS mainly relies on pathological morphology and immunohistochemical staining combined with clinical symptoms. However, whether histiocytic sarcoma can be detected using flow cytometry (FCM) is not clear. We report the case of a 61-year-old female patient with a group of abnormal cells identified primarily by FCM. Largeabnormal cells -CD68+CD11c+CD14+HLA-DR+CD123+CD45+presented in upper forward scatter and side scatter, indicating that a malignancy originally derived from monocyte/macrophage could be histocytic sarcoma. This patient with histiocytic sarcoma was finally confirmed with pathology and immunohistochemistry. In conclusion, HS can be detected by assessment of CD68+CD11c+CD14+HLA-DR+CD123+CD45+cells using FCM, suggesting that FCM can be an effective tool for early finding of HS. © 2015 International Clinical Cytometry Society.
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Jiakui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Tao
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Hengjuan
authors.initials: H
authors.lastname: Bian
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tao
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Chunhuai
authors.initials: C
authors.lastname: Wang
authors.affiliation: Centre for Transplant and Renal Research, Westmead Millennium Institute at University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hamatological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230601, People's Republic of China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zhai
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D054747:Histiocytic Sarcoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000473:pathology
chemical_list: D015703:Antigens, CD
keywords: flow cytometry; histiocytic sarcoma; immunohistochemistry; pathological morphology