title: Efficient Separation and Analysis of Triacylglycerols: Quantitation of β-Palmitate (OPO) in Oils and Infant Formulas.
abstract: A high-efficiency, convenient, and reliable method for the separation of structurally similar triacylglycerols is detailed and applied in the quantitative analysis of 1,3-dioleoyl-2-palmitoylglycerol (OPO) in infant formulas and OPO oils. OPO is an important lipid component in "humanized" infant formula. A fast preparative isolation of an OPO-containing fraction from the crude complex mixture, by nonaqueous reversed phase HPLC, followed by Ag(+)-HPLC with detection at 205 nm allowed fine separation and detection of the desired fraction. OPO was quantitated independently of its regioisomer 1,2-dioleoyl-3-palmitoylglycerol (OOP) and isomers of stearoyl-linoleoyl-palmitoyl glycerol that might be present in infant formulas. For samples with low OPO content, an evaporative light-scattering detector (ELSD) was more preferable than UV detection, with a calculated LOD of 0.1 μg of OPO injected and LOQ of 0.3 μg. The method, which showed high reproducibility (RSD < 5%), was suitable for both high OPO content oils and low OPO products such as unenriched infant formula. A number of possible interference issues were considered and dealt with.
authors.affiliation: †Callaghan Innovation, 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Vyssotski
authors.affiliation: †Callaghan Innovation, 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Bloor
authors.affiliation: †Callaghan Innovation, 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand.
authors.forename: Kirill
authors.initials: K
authors.lastname: Lagutin
authors.affiliation: †Callaghan Innovation, 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand.
authors.forename: Herbert
authors.initials: H
authors.lastname: Wong
authors.affiliation: †Callaghan Innovation, 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand.|§Gracefield Research Centre, Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5010, New Zealand.
authors.forename: D Bradley G
authors.initials: DB
authors.lastname: Williams
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D041943:Infant Formula / Q000737:chemistry*; D010168:Palmitates / Q000032:analysis* / Q000302:isolation & purification; D010938:Plant Oils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D010168:Palmitates; D010938:Plant Oils; D014280:Triglycerides; C095376:1,3-dioleoyl-2-palmitoylglycerol
keywords: ELSD; HPLC; fatty acid; infant formula; regiospecific analysis; triacylglycerol