title: Increased expression of microRNA-196a predicts poor prognosis in human ovarian carcinoma.
abstract: OBJECTIVE
  Overexpression of MicroRNA-196a (miR-196a) has recently been reported in different types of human cancers. However, the prognostic value of miR-196a in ovarian carcinoma remains unknown. In this study, we investigated the expression of miR-196a in ovarian carcinoma and its relationship with tumor progression and clinical prognosis.
  
  
  METHODS
  The expression level of miR-196a was examined by quantitative Real-time PCR (qRT-PCR) in surgically removed ovarian cancer tissues and ovarian cancer cell lines. The correlation between miR-196a expression and clinical features and prognosis were statistically analyzed.
  
  
  RESULTS
  The results showed that the miR-196a expression was significantly upregulated in tumor tissues and ovarian cancer cell lines compared with that in normal ovarian surface tissues and normal ovarian epithelial cells. Moreover, miR-196a expression was positively correlated with FIGO stage (P<0.001), tumor size (P=0.020), and lymph nodes metastasis (P=0.019). Kaplan-Meier analysis demonstrated that high levels of miR-196a expression was associated with poorer overall survival (P<0.001) and recurrent-free survival (P=0.003), especially in patients with advanced disease (P=0.002). Multivariate analysis suggested that miR-196a expression was an independent prognostic factor for overall survival of patients with ovarian carcinoma.
  
  
  CONCLUSIONS
  In conclusion, miR-196a may play an important role in the progression of ovarian carcinoma, and could be used as an independent prognostic biomarker for patients with ovarian carcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Childen's Medical Center, Guangzhou Medical University Guangzhou, China.
authors.forename: Jiezhen
authors.initials: J
authors.lastname: Huang
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D045744:Cell Line, Tumor; D016009:Chi-Square Distribution; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: miR-196a; ovarian cancer; overall survival; recurrence-free survival