title: Nanostructured lipid carrier based topical gel of Ganoderma Triterpenoids for frostbite treatment.
abstract: The objective of this study was to prepare nanostructured lipid carrier (NLC)-based topical gel of Ganoderma Triterpenoids (GTs) and evaluate their effects on frostbite treatment. GT-NLCs was prepared by the high pressure homogenization method and then characterized by morphology and analyses of particle size, zeta potential, entrapment efficiency (EE), and drug loading (DL). The NLCs was suitably gelled for skin permeation studies in vitro and pharmacodynamic evaluation in vivo, compared with the GT emulgel. The GT-NLC remained within the colloidal range and was uniformly dispersed after suitably gelled by carbopol preparation. Transmission electron microscopy (TEM) study showed GT-NLCs was spherical in shape. The EE (%) and DL (%) could reach up to (81.84 ± 0.60)% and (2.13 ± 0.12)%, respectively. The result of X-ray diffractograms (XRD) showed that GTs were in an amorphous state in the NLC-gel. In vitro permeation studies through rat skin indicated that the amount of GTs permeated through skin of GT-NLCs after 24 h was higher than that of GT emulsion, and GT-NLCs increased the accumulative amounts of GTs in epidermis 7.76 times greater than GT emulsion. GT-NLC-gel was found to possess superior therapeutic effect for frostbite, compared with the GT emulgel. The NLC based topical gel of GTs could improve -their therapeutic effect for frostbite.
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Shen
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China.
authors.forename: Bao-De
authors.initials: BD
authors.lastname: Shen
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Bai
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: He
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Lv
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China. Electronic address: hanjin302emba@163.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Pharmacy, 302 Military Hospital, Beijing 100039, China. Electronic address: yhlpharm@126.com.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Yuan
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry; D005627:Frostbite / Q000188:drug therapy*; D032902:Ganoderma / Q000737:chemistry*; D005782:Gels / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008297:Male; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D005782:Gels; D008055:Lipids
keywords: Frostbite; Ganoderma Triterpenes; High pressure homogenization; Nanostructured lipid carriers; Topical gel