title: Vaccination with cyclin-dependent kinase tick antigen confers protection against Ixodes infestation.
abstract: Among arthropods, ticks lead as vectors of animal diseases and rank second to mosquitoes in transmitting human pathogens. Cyclin-dependent kinases (CDK) participate in cell cycle control in eukaryotes. CDKs are serine/threonine protein kinases and these catalytic subunits are activated or inactivated at specific stages of the cell cycle. To determine the potential of using CDKs as anti-tick vaccine antigens, hamsters were immunized with recombinant Ixodes persulcatus CDK10, followed by a homologous tick challenge. Though it was not exactly unexpected, IpCDK10 vaccination significantly impaired tick blood feeding and fecundity, which manifested as low engorgement weights, poor oviposition, and a reduction in 80% of hatching rates. These findings may underpin the development of more efficacious anti-tick vaccines based on the targeting of cell cycle control proteins.
authors.affiliation: Laboratório Integrado de Bioquímica Hatisaburo Masuda, NUPEM - UFRJ, Campus Macaé, Avenida São José do Barreto, São José do Barreto, Macaé, RJ CEP 27971-220, Brazil. Electronic address: hgomes2@yahoo.com.br.
authors.forename: Helga
authors.initials: H
authors.lastname: Gomes
authors.affiliation: Laboratório Integrado de Bioquímica Hatisaburo Masuda, NUPEM - UFRJ, Campus Macaé, Avenida São José do Barreto, São José do Barreto, Macaé, RJ CEP 27971-220, Brazil; Instituto Nacional de Ciência e Tecnologia - Entomologia Molecular, Rio de Janeiro, RJ, Brazil; Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, CCS, Bloco H, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Moraes
authors.affiliation: Tick Vector Laboratory, International Livestock Research Institute, P.O. Box 30709-00100, Nairobi, Kenya.
authors.forename: Naftaly
authors.initials: N
authors.lastname: Githaka
authors.affiliation: Laboratório de Química e Função de Proteínas e Peptídeos, Unidade de Experimentação Animal - CBB - UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes RJ, CEP 28015-620, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Martins
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-Ku, Sapporo 060-0818, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Isezaki
authors.affiliation: Centro de Biotecnologia e Faculdade de Veterinária, UFRGS, Avenida Bento Gonçalves, 9500, Porto Alegre, RS C.P. 15005, CEP 91501-970, Brazil; Instituto Nacional de Ciência e Tecnologia - Entomologia Molecular, Rio de Janeiro, RJ, Brazil.
authors.forename: Itabajara da Silva
authors.initials: Ida S
authors.lastname: Vaz
authors.affiliation: Laboratório de Química e Função de Proteínas e Peptídeos, Unidade de Experimentação Animal - CBB - UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes RJ, CEP 28015-620, Brazil; Instituto Nacional de Ciência e Tecnologia - Entomologia Molecular, Rio de Janeiro, RJ, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Logullo
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-Ku, Sapporo 060-0818, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-Ku, Sapporo 060-0818, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D006224:Cricetinae; D018844:Cyclin-Dependent Kinases / Q000276:immunology* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005786:Gene Expression Regulation; D018884:Ixodes / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D013984:Tick Infestations / Q000517:prevention & control*; D059467:Transcriptome; D014612:Vaccines / Q000276:immunology*
chemical_list: D000941:Antigens; D011994:Recombinant Proteins; D014612:Vaccines; D012313:RNA; D018844:Cyclin-Dependent Kinases
keywords: CDK; Cell cycle; Ixodes persulcatus; Tick; Vaccine