title: Effects of varying local temperature on the optical properties of cells in-vitro.
abstract: Increase in local temperature during light exposure of biological tissues plays an important role in determining the fate of most therapeutic modalities. Variations in the optical properties (absorption coefficient, scattering coefficient, anisotropy factor, optical depth etc.) of two cancer cell lines "Rhobdomyosarcoma and Cervical carcinoma" due to gradual increase in temperature were determine quantitatively with a double integrating sphere system. It was observed that all three coefficients showed decreasing tendency as the temperature increases for both the cell lines except for scattering coefficient of HeLa which remain constant within error limit. Anisotropy factor for both cell lines increased indicating temperature dependent subcellular density variations. Temperature dependent optical properties information may lead to precise dosimetry and could help clinicians for predicting the therapeutic modality outcome.
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan; Center for Nuclear Medicine and Radiotherapy (CENAR), Brewery Road, P.O. Box 17, Quetta, Pakistan. Electronic address: iahmadmp@gmail.com.
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan. Electronic address: abdulrehmanphy01@gmail.com.
authors.forename: Abdul
authors.initials: A
authors.lastname: Rehman
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan. Electronic address: fac268@pieas.edu.pk.
authors.forename: Junaid A
authors.initials: JA
authors.lastname: Khan
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rafi
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan. Electronic address: ahmat82@gmail.com.
authors.forename: Ahmat
authors.initials: A
authors.lastname: Khurshid
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan. Electronic address: hasanisar@gmail.com.
authors.forename: Hasan
authors.initials: H
authors.lastname: Nisar
authors.affiliation: National Institute of Health, Park Road, Islamabad, Pakistan.
authors.forename: S S Z
authors.initials: SS
authors.lastname: Zaidi
authors.affiliation: Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, 45650, Islamabad, Pakistan. Electronic address: masroor@pieas.edu.pk.
authors.forename: Masroor
authors.initials: M
authors.lastname: Ikram
mesh_terms: D016880:Anisotropy; D045744:Cell Line, Tumor; D006358:Hot Temperature; D006801:Humans; D009369:Neoplasms / Q000503:physiopathology*; D055620:Optical Phenomena
keywords: Absorption coefficient; Anisotropy factor; Biomedical; Optical properties; Scattering coefficient