title: Profiling microbial community structures across six large oilfields in China and the potential role of dominant microorganisms in bioremediation.
abstract: Successful bioremediation of oil pollution is based on a comprehensive understanding of the in situ physicochemical conditions and indigenous microbial communities as well as the interaction between microorganisms and geochemical variables. Nineteen oil-contaminated soil samples and five uncontaminated controls were taken from six major oilfields across different geoclimatic regions in China to investigate the spatial distribution of the microbial ecosystem. Microbial community analysis revealed remarkable variation in microbial diversity between oil-contaminated soils taken from different oilfields. Canonical correspondence analysis (CCA) further demonstrated that a suite of in situ geochemical parameters, including soil moisture and sulfate concentrations, were among the factors that influenced the overall microbial community structure and composition. Phylogenetic analysis indicated that the vast majority of sequences were related to the genera Arthrobacter, Dietzia, Pseudomonas, Rhodococcus, and Marinobacter, many of which contain known oil-degrading or oil-emulsifying species. Remarkably, a number of archaeal genera including Halalkalicoccus, Natronomonas, Haloterrigena, and Natrinema were found in relatively high abundance in some of the oil-contaminated soil samples, indicating that these Euryarchaeota may play an important ecological role in some oil-contaminated soils. This study offers a direct and reliable reference of the diversity of the microbial community in various oil-contaminated soils and may influence strategies for in situ bioremediation of oil pollution.
authors.affiliation: Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, 572000, China.|Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, 08901, NJ, USA.
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
authors.affiliation: Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, 572000, China. lijiwei20025@sidsse.ac.cn.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Li
authors.affiliation: Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, 572000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Qingdao Institute of Marine Geology, Qingdao, 266071, China.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Energy Resources, Chengdu University of Technology, Chengdu, 610059, China.
authors.forename: Meiyan
authors.initials: M
authors.lastname: Fu
authors.affiliation: Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, 572000, China. xtpeng@sidsse.ac.cn.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Peng
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D058448:Biota; D002681:China; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields; D059392:Petroleum Pollution; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Crude oil bioremediation; Illumina sequencing; Microbial community analysis; Oil-degrading bacteria; Oil-emulsifying bacteria