title: Central Administration of the Ciliary Neurotrophic Factor Analogue, Axokine, Does Not Play a Role in Long-Term Energy Homeostasis in Adult Mice.
abstract: Ciliary neurotrophic factor (CNTF) exerts powerful anorectic effects and has been suggested to regulate long-term energy balance by inducing adult neurogenesis in the arcuate nucleus of the hypothalamus.
  
  
  
  The CNTF analogue, Axokine, was infused into the lateral ventricle of high-fat-fed mice for 1 week. Food intake, energy expenditure, body mass, glucose metabolism, and neurogenesis in the arcuate nucleus (ARC) of the hypothalamus were assessed 3 weeks after cessation of Axokine treatment.
  
  
  
  Short-term administration of Axokine induced an anorexic response but did not promote sustained weight loss. Instead, a rapid rebound in food intake and body mass occurred immediately after cessation of Axokine treatment, and this tended to reduce insulin sensitivity. Immunolabeling of 5-bromo-2'-deoxyuridine revealed limited neurogenesis in the ARC 3 weeks after Axokine treatment.
  
  
  
  These findings suggest that Axokine/CNTF does not induce substantial or sustained ARC neurogenesis or contribute to the long-term regulation of energy balance in mice.
authors.affiliation: Department of Physiology, Monash University, Clayton, Vic., Australia.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Borg
authors.forename: Alex
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Moyra
authors.initials: M
authors.lastname: Lemus
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Oldfield
authors.forename: Zane B
authors.initials: ZB
authors.lastname: Andrews
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Watt
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007276:Injections, Intraventricular; D007328:Insulin / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000187:drug effects*; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D013997:Time Factors
chemical_list: D020934:Ciliary Neurotrophic Factor; D007328:Insulin; D009478:Neuropeptide Y; D049452:Green Fluorescent Proteins; C118937:axokine; D005947:Glucose