title: Aeration and fermentation strategies on nisin production.
abstract: OBJECTIVE
  To optimize nisin production in Lactococcus lactis by using different aeration and fermentation strategies.
  
  
  RESULTS
  The nisin titer and specific nisin production rate reached maximum values of 11,900 IU/ml and 4110 IU/g/h, respectively, in aerobic batch fermentation with glucose as C source. These values were higher than in anaerobic batch fermentation (10,700 IU/ml and 3260 IU/g/h, respectively). The maximum specific nisin production rates appeared earlier in aerobic batch fermentation, which suggests that nisin production is stimulated by aeration. Different fermentation strategies were compared: maximum nisin production (15,400 IU/ml) was achieved with fed-batch fermentation with a variable rate of feeding under aerobic conditions.
  
  
  CONCLUSIONS
  Nisin production can be stimulated by aeration, which goes against the typical conditions involving strict anaerobiosis.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130023, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Jiang
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Yan
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Qiyue
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Zaixiao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Qingfan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China. renxiaodong@jlu.edu.cn.|Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130023, China. renxiaodong@jlu.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ren
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D009561:Nisin / Q000378:metabolism*
chemical_list: D009561:Nisin; D002244:Carbon; D005947:Glucose
keywords: Aeration; Batch fermentation; Fed batch fermentation; Glucose; Lactococcus lactis; Nisin