title: [Effect of andrographolide on quorum sensing and relevant virulence genes of Candida albicans].
abstract: OBJECTIVE
  To investigate the effect of andrographolide (AG) on quroum sensing (QS) and relevant virulence genes of Candida albicans.
  
  
  METHODS
  Gas-chromatography-mass spectrometry (GC-MS) was applied to detect the changes in the content of farnesol and tyrosol in C. albicans intervened by AG. The real-time quantitative PCR (qRT-PCR) was adopted to inspect the expressions of relevant virulence genes such as CHK1, PBS2 and HOG1 regulated by QS.
  
  
  RESULTS
  At 2 h after the growth of C. albican, the farnesol and tyrosol secretions reduced, without notable change after intervention with AG. The secretions were highest at 12 h and decreased at 24 h. After the intervention with different concentrations of AG, the farnesol content reduces, whereas tyrosol increased, indicating a dose-dependence, particularly with 1 000 mg x L(-1) AG. qRT-PCR revealed that 1 000 mg x L(-1) AG could down-regulate CHK1 by 2.375, 3.330 and 4.043 times and PBS2 by 2.010, 4.210 and 4.760 times, with no significant change in HOG1.
  
  
  CONCLUSIONS
  AG could inhibit the farnesol secretion, promote the tyrosol secretion and down-regulate QS-related virulence genes CHK1 and PBS2 expressions.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Yan
authors.forename: Gao-xiang
authors.initials: GX
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Ke-qiao
authors.initials: KQ
authors.lastname: Lu
authors.forename: Meng-xiang
authors.initials: MX
authors.lastname: Zhang
authors.forename: Tian-ming
authors.initials: TM
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Chang-zhong
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D004224:Diterpenes / Q000494:pharmacology*; D005204:Farnesol / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005800:Genes, Fungal; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D053038:Quorum Sensing / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics*
chemical_list: D004224:Diterpenes; C011867:4-hydroxyphenylethanol; C030419:andrographolide; D005204:Farnesol; D010626:Phenylethyl Alcohol