title: Radioprotective effects of genistein on HL-7702 cells via the inhibition of apoptosis and DNA damage.
abstract: Radiation induced normal tissue damage is the most important limitation for the delivery of a high potentially curative radiation dose. Genistein (GEN), one of the main soy isoflavone components, has drawn wide attention for its bioactivity in alleviating radiation damage. However, the effects and molecular mechanisms underlying the radioprotective effects of GEN remain unclear. In the present study, we showed that low concentration of GEN (1.5 µM) protected L-02 cells against radiation damage via inhibition of apoptosis, alleviation of DNA damage and chromosome aberration, down-regulation of GRP78 and up-regulation of HERP, HUS1 and hHR23A. In contrast, high concentration of GEN (20 µM) demonstrated radiosensitizing characteristics through the promotion of apoptosis and chromosome aberration, impairment of DNA repair, up-regulation of GRP78, and down-regulation of HUS1, SIRT1, RAD17, RAD51 and RNF8. These findings shed light on using low, but not high-concentration GEN, as a potential candidate for adjuvant therapy to alleviate radiation-induced injuries to human recipients of ionizing radiation.
authors.affiliation: Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Research Center for Food Safety and Nutrition, Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Oncology, Shanghai Tongren Hospital, Shanghai Jiaotong University, Shanghai 200336, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Life Science and Technology, Shanghai Jiao Tong University, Shanghai 200020, China.
authors.forename: Fengsong
authors.initials: F
authors.lastname: Cong
authors.affiliation: Nutrition Department, School of Medicine, Shanghai Jiao Tong University, Shanghai 200020, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Shen
authors.affiliation: Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Research Center for Food Safety and Nutrition, Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Jing
authors.affiliation: Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Research Center for Food Safety and Nutrition, Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Ying
authors.affiliation: Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Research Center for Food Safety and Nutrition, Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Haiyue
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Research Center for Food Safety and Nutrition, Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Medicine, Changhai Hosipital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Yongye
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Laboratory Medicine, Changhai Hosipital, Second Military Medical University, Shanghai 200433, China. Electronic address: hongliyan@smmu.edu.cn.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yan
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004249:DNA Damage; D045643:DNA Repair Enzymes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D000091342:Endoplasmic Reticulum Chaperone BiP; D019833:Genistein / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014965:X-Rays
chemical_list: D004268:DNA-Binding Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; C485932:HERPUD1 protein, human; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D011837:Radiation-Protective Agents; C086985:RAD23A protein, human; D019833:Genistein; D045643:DNA Repair Enzymes
keywords: Apoptosis; Chromosome aberrations; DNA damage; Genistein; Radioprotective