title: LOV-based reporters for fluorescence imaging.
abstract: Chromophore-binding domains from plant and bacterial photoreceptor proteins have recently gathered increasing attention as new sources of genetically encoded fluorescent proteins (FPs). In particular, FPs based on the flavin-binding LOV (light, oxygen, or voltage sensing) domain offer advantages over green fluorescent protein (GFP) owing to their smaller size, pH and thermal stability, utility under anaerobic conditions and their ability to generate reactive oxygen species. This review focuses on the potential applications of this emerging class of fluorescent reporters, discusses the advantages and limitations of LOV-based FPs, whilst offering insights regarding the further development of this technology for bioimaging and photodynamic therapy.
authors.affiliation: Institute of Infection, Immunology & Inflammation, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Buckley
authors.affiliation: Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Jan
authors.initials: J
authors.lastname: Petersen
authors.affiliation: Institute of Infection, Immunology & Inflammation, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Roe
authors.affiliation: Institute of Infection, Immunology & Inflammation, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Gillian R
authors.initials: GR
authors.lastname: Douce
authors.affiliation: Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK. Electronic address: John.Christie@glasgow.ac.uk.
authors.forename: John M
authors.initials: JM
authors.lastname: Christie
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods*; D004138:Dinitrocresols / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D057054:Molecular Imaging / Q000379:methods*; D010100:Oxygen / Q000737:chemistry; D011485:Protein Binding; D055550:Protein Stability; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004138:Dinitrocresols; D005456:Fluorescent Dyes; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins; C024132:4,6-dinitro-o-cresol; D010100:Oxygen