title: Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix.
abstract: We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of 3.72μm and an axial resolution of 5μm, across a wide field-of-view of 24mm2. We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments.
authors.forename: Chao
authors.initials: C
authors.lastname: Zuo
authors.forename: Jiasong
authors.initials: J
authors.lastname: Sun
authors.forename: Jialin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D001199:Ascaridoidea / Q000166:cytology; D002610:Cheek; D006801:Humans; D021621:Imaging, Three-Dimensional; D007909:Lenses; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000379:methods*; D008863:Microspheres; D019904:Polymethyl Methacrylate / Q000737:chemistry; D012031:Refractometry; D014054:Tomography / Q000379:methods*
chemical_list: D019904:Polymethyl Methacrylate