title: The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus).
abstract: Pea streak virus (PeSV) is a member of the genus Carlavirus in the family Betaflexiviridae. Here, the first complete genome sequence of PeSV was determined by deep sequencing of a cDNA library constructed from dsRNA extracted from a PeSV-infected sample and Rapid Amplification of cDNA Ends (RACE) PCR. The PeSV genome consists of 8041 nucleotides excluding the poly(A) tail and contains six open reading frames (ORFs). The putative peptide encoded by the PeSV ORF6 has an estimated molecular mass of 6.6 kDa and shows no similarity to any known proteins. This differs from typical carlaviruses, whose ORF6 encodes a 12- to 18-kDa cysteine-rich nucleic-acid-binding protein.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.|Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, V0H 1Z0, Canada.|College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Zhengnan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Mike
authors.initials: M
authors.lastname: Bernardy
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Wiersma
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. chengzh@nwsuaf.edu.cn.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, V0H 1Z0, Canada. yu.xiang@agr.gc.ca.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D001483:Base Sequence; D017788:Carlavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018532:Pisum sativum / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*