title: ICAM-1 and VCAM-1 are differentially expressed on blood-retinal barrier cells during experimental autoimmune uveitis.
abstract: Adhesion molecules play a central role in leukocyte adhesion to the blood-retinal barrier (BRB) during uveitis. VCAM-1 expression on the BRB has been already described but although structurally similar, ICAM-1 has shown in various autoimmunity models to have distinct role and expression. Here, we induced uveitis in C57Bl/6 mice by adoptive transfer of semi-purified T cells from IRBP1-20-immunized mice. Using Flow cytometry analysis on transferred cells and immunofluorescence staining on retina we have studied the comparative ocular expression of both ICAM-1 and VCAM-1 and their ligands LFA-1 and VLA-4 at the surface of uveitogenic cells. Our results showed that LFA-1 and VLA-4 are expressed on both T and non T cells, VLA-4 sparsely and LFA-1 ubiquitously. Considering retinal expression, ICAM-1 is faintly present and VCAM-1 is absent in naive eyes. Only ICAM-1 is present on infiltrating cells in the retina and vitreous, while only VCAM-1 extends to perivascular glial cells and all along the internal limiting membrane. Finally, ICAM-1 is strongly expressed on the RPE, where VCAM-1 expression is much weaker. VCAM-1 seems most strongly expressed on the internal BRB while ICAM-1 predominates on the external BRB. Those major differences in the expression pattern could represent differential entry pathways for inflammatory cells to penetrate the eye.
authors.affiliation: I.R.I.B.H.M (Institute of Interdisciplinary Research), Université Libre de Bruxelles Campus Erasme, Brussels, Belgium; Department of Ophthalmology, CHU Saint-Pierre and Brugmann, Brussels, Belgium. Electronic address: rdewispe@ulb.ac.be.
authors.forename: R
authors.initials: R
authors.lastname: Dewispelaere
authors.affiliation: I.R.I.B.H.M (Institute of Interdisciplinary Research), Université Libre de Bruxelles Campus Erasme, Brussels, Belgium; Department of Ophthalmology, CHU Erasme, Brussels, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Lipski
authors.affiliation: I.R.I.B.H.M (Institute of Interdisciplinary Research), Université Libre de Bruxelles Campus Erasme, Brussels, Belgium; Department of Ophthalmology, CHU Saint-Pierre and Brugmann, Brussels, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Foucart
authors.affiliation: I.R.I.B.H.M (Institute of Interdisciplinary Research), Université Libre de Bruxelles Campus Erasme, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Bruyns
authors.affiliation: Department of Ophthalmology, CHU Saint-Pierre and Brugmann, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Frère
authors.affiliation: Department of Ophthalmology, CHU Saint-Pierre and Brugmann, Brussels, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Caspers
authors.affiliation: I.R.I.B.H.M (Institute of Interdisciplinary Research), Université Libre de Bruxelles Campus Erasme, Brussels, Belgium; Department of Ophthalmology, CHU Saint-Pierre and Brugmann, Brussels, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Willermain
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000378:metabolism*; D001813:Blood-Retinal Barrier; D002448:Cell Adhesion; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D014605:Uveitis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1
keywords: Autoimmunity; Blood retina barrier; EAU; IRBP; Uveitis