title: An influenza viral vector Brucella abortus vaccine induces good cross-protection against Brucella melitensis infection in pregnant heifers.
abstract: Brucella melitensis can be transmitted and cause disease in cattle herds as a result of inadequate management of mixed livestock farms. Ideally, vaccines against Brucella abortus for cattle should also provide cross-protection against B. melitensis. Previously we created a novel influenza viral vector B. abortus (Flu-BA) vaccine expressing the Brucella ribosomal proteins L7/L12 or Omp16. This study demonstrated Flu-BA vaccine with adjuvant Montanide Gel01 provided 100% protection against abortion in vaccinated pregnant heifers and good cross-protection of the heifers and their calves or fetuses (90-100%) after challenge with B. melitensis 16M; the level of protection provided by Flu-BA was comparable to the commercial vaccine B. abortus S19. In terms of the index of infection and colonization of Brucella in tissues, both vaccines demonstrated significant (P=0.02 to P<0.0001) protection against B. melitensis 16M infection compared to the negative control group (PBS+Montanide Gel01). Thus, we conclude the Flu-BA vaccine provides cross-protection against B. melitensis infection in pregnant heifers.
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409 Gvardeiskiy, Kazakhstan. Electronic address: tabynov_81@mail.ru.
authors.forename: Kaissar
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409 Gvardeiskiy, Kazakhstan.
authors.forename: Sholpan
authors.initials: S
authors.lastname: Ryskeldinova
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409 Gvardeiskiy, Kazakhstan.
authors.forename: Abylai
authors.initials: A
authors.lastname: Sansyzbay
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000517:prevention & control*; D002417:Cattle; D056738:Cross Protection; D004337:Drug Carriers; D005260:Female; D009975:Orthomyxoviridae / Q000235:genetics*; D011247:Pregnancy; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D002004:Brucella Vaccine; D004337:Drug Carriers; D014614:Vaccines, Synthetic
keywords: Brucella melitensis; Cross-protection; Influenza viral vector; Pregnant heifer; Vaccine