title: Functional Cell Adhesion Receptors (Integrins) in Polymeric Architectures.
abstract: Integrins, as transmembrane heterodimeric receptors, have important functions in cell adhesion, migration, proliferation, survival apoptosis and signal transduction, in many physio- as well as pathophysiological settings. Characterisation of integrins and their ligand/antagonist binding is notoriously difficult, due to high integrin redundancy and ubiquity. Bypassing the intrinsic difficulties of cell-based integrin expression, purification and reconstitution, we present for the first time the synthesis of a heterodimeric integrin receptor and its assembly into a block-copolymeric membrane mimic. We present comprehensive data to demonstrate the synthesis of functionally active integrin αv β3, generated by in vitro membrane-assisted protein synthesis (iMAPS). This work represents the first step towards a robust and adaptable polymer-based platform for characterisation of integrin-ligand interactions.
authors.affiliation: University of Natural Resources and Life Sciences, BOKU, Department of Nanobiotechnology, Institute for Synthetic Bioarchitectures, Muthgasse 11, 1190 Vienna (Austria).
authors.forename: Christoph
authors.initials: C
authors.lastname: Zaba
authors.affiliation: Institute for Molecular Biology (IMB), Core Facility Microscopy, Ackermannweg 4, 55128 Mainz (Germany).
authors.forename: Sandra
authors.initials: S
authors.lastname: Ritz
authors.affiliation: University of Natural Resources and Life Sciences, BOKU, Department of Nanobiotechnology, Institute for Synthetic Bioarchitectures, Muthgasse 11, 1190 Vienna (Austria).
authors.forename: Cherng-Wen Darren
authors.initials: CW
authors.lastname: Tan
authors.affiliation: University of Natural Resources and Life Sciences, BOKU, Department of Nanobiotechnology, Institute for Synthetic Bioarchitectures, Muthgasse 11, 1190 Vienna (Austria).
authors.forename: Sonja
authors.initials: S
authors.lastname: Zayni
authors.affiliation: Clinical Research Unit, Department of Obstetrics and Gynecology, Technische Universität München, Ismaninger Strasse 22, 81675 Munich (Germany).
authors.forename: Martina
authors.initials: M
authors.lastname: Müller
authors.affiliation: Clinical Research Unit, Department of Obstetrics and Gynecology, Technische Universität München, Ismaninger Strasse 22, 81675 Munich (Germany). ute.reuning@lrz.tum.de.
authors.forename: Ute
authors.initials: U
authors.lastname: Reuning
authors.affiliation: University of Natural Resources and Life Sciences, BOKU, Department of Nanobiotechnology, Institute for Synthetic Bioarchitectures, Muthgasse 11, 1190 Vienna (Austria). eva.sinner@boku.ac.at.
authors.forename: Eva-Kathrin
authors.initials: EK
authors.lastname: Sinner
mesh_terms: D002448:Cell Adhesion; D002474:Cell-Free System; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D018613:Microscopy, Confocal; D008958:Models, Molecular; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000138:chemical synthesis / Q000737:chemistry; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000737:chemistry / Q000378:metabolism*; D017510:Protein Folding
chemical_list: D016023:Integrins; D010713:Phosphatidylcholines; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; C017251:1,2-oleoylphosphatidylcholine
keywords: block copolymers; cell-free in vitro protein synthesis; integrins; membrane proteins; polymersomes