title: VirB6 and VirB10 from the Brucella type IV secretion system interact via the N-terminal periplasmic domain of VirB6.
abstract: Type IV secretion systems are multi-protein complexes that transfer macromolecules across the cell envelope of bacteria. Identifying the sites of interaction between the twelve proteins (VirB1-VirB11 and VirD4) that form these complexes is key to understanding their assembly and function. We have here used phage display, bacterial two-hybrid and fluorescence-based interaction assays to identify an N-terminal domain of the inner membrane protein VirB6 as a site of interaction with the envelope-spanning VirB10 protein. Our results are consistent with the notion that VirB6 acts in concert with VirB10 as well as with VirB8 during secretion system assembly and function.
authors.affiliation: Université de Montréal, Pavillon Roger-Gaudry, Department of Biochemistry and Molecular Medicine, C.P. 6128, Succ. Centre-Ville, Montréal, QC H3C 3J7, Canada.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Villamil Giraldo
authors.affiliation: Université de Montréal, Pavillon Roger-Gaudry, Department of Biochemistry and Molecular Medicine, C.P. 6128, Succ. Centre-Ville, Montréal, QC H3C 3J7, Canada.
authors.forename: Charline
authors.initials: C
authors.lastname: Mary
authors.affiliation: Université de Montréal, Pavillon Roger-Gaudry, Department of Biochemistry and Molecular Medicine, C.P. 6128, Succ. Centre-Ville, Montréal, QC H3C 3J7, Canada.
authors.forename: Durgajini
authors.initials: D
authors.lastname: Sivanesan
authors.affiliation: Université de Montréal, Pavillon Roger-Gaudry, Department of Biochemistry and Molecular Medicine, C.P. 6128, Succ. Centre-Ville, Montréal, QC H3C 3J7, Canada. Electronic address: christian.baron@umontreal.ca.
authors.forename: Christian
authors.initials: C
authors.lastname: Baron
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001435:Bacteriophages / Q000235:genetics; D001665:Binding Sites; D002002:Brucella / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D019897:Periplasm / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D049452:Green Fluorescent Proteins
keywords: Bacterial virulence; Membrane protein; Protein–protein interaction; Type IV secretion system