title: One-Step Vital Staining of Presynaptic Terminals and Post-Synaptic Receptors at Neuromuscular Junctions in Mouse Skeletal Muscle.
abstract: This protocol describes vital staining of neuromuscular junctions in the mouse triangularis sterni muscle in one incubation step, combining presynaptic, motor nerve terminal staining with the styryl dye FM1-43, which labels recycling synaptic vesicles, and TRITC-α-bungarotoxin, which labels acetylcholine receptors in the motor endplate membrane. Curr. Protoc. Mouse Biol. 1:489-496 © 2011 by John Wiley & Sons, Inc.
authors.affiliation: Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Ribchester
keywords: acetylcholine receptor; fluorescence microscopy; neuromuscular junction; synaptic vesicle; valuestyryl dyes; vital staining; α-bungarotoxin