title: Effects of 2-(4-Methoxyphenyl)-5, 6-trimethylene-4H-1, 3, 2-oxathiaphosphorine-2-sulfide on biomarkers of Mediterranean clams Ruditapes decussatus.
abstract: The effects of exposure to a novel synthetic organophosphorus compound, 2-(4-Methoxyphenyl)-5, 6-trimethylene-4H-1, 3, 2-oxathiaphosphorine-2-sulfide (OMTOS) concentrations (Control=0, C1=0.01, C2=0.1, C3=1 and C4=10μg/L) were investigated in the clam Ruditapes decussatus. Vitellogenin (Vg)-like protein levels in haemolymph from males and females were investigated. Concentrations of 1μg/L and 10μg/L significantly decreased Vg levels in male haemolymph after 7 days, whereas significant variations were only found in females treated with 10μg/L. On the other hand, superoxide dismutase (SOD), catalase (CAT) and acetylcholinesterase activities (AChE) in whole soft tissue were measured after 2, 4 and 7 days of exposure to the same series of concentrations. After 2 days of exposure, 0.1, 1, and 10μg/L of OMTOS increased SOD activity significantly, but this decreased with 10μg/L after 4 and 7 days. No changes in CAT activity were observed after 2 days compared to controls. OMTOS significantly reduced AChE activity after 4 and 7 days in treated clams with the highest concentration 10μg/L, but it did not induce significant variations at the other concentrations tested. Our study demonstrates that OMTOS alters biochemical parameters in R. decussatus, even at low concentrations, and suggests differing modes of action of the contaminant. Using clams is a powerful tool to provide valuable insights into possible mechanisms of environmental toxicity of novel synthetic organic products both in non-target organisms and the marine ecosystem. Additionally, our results highlight that biomarker responses facilitate elucidation of putative mechanisms of action of OMTOS in non-target species.
authors.affiliation: Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
authors.forename: Badreddine
authors.initials: B
authors.lastname: Sellami
authors.affiliation: Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerta, University of Carthage, 7021 Jarzouna, Tunisia. Electronic address: aouani_iyadh@yahoo.fr.
authors.forename: Iyadh
authors.initials: I
authors.lastname: Aouani
authors.affiliation: Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
authors.forename: Aziza
authors.initials: A
authors.lastname: Maalaoui
authors.affiliation: Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Dellali
authors.affiliation: Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
authors.forename: Patricia
authors.initials: P
authors.lastname: Aïssa
authors.affiliation: Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerta, University of Carthage, 7021 Jarzouna, Tunisia.
authors.forename: Soufiane
authors.initials: S
authors.lastname: Touil
authors.affiliation: Environmental Research Institute and School of Biochemistry and Cell Biology, University College Cork, Western Gateway Building, Western Road, Cork, Ireland.
authors.forename: David
authors.initials: D
authors.lastname: Sheehan
authors.affiliation: Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
authors.forename: Ezzeddine
authors.initials: E
authors.lastname: Mahmoudi
authors.affiliation: Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
authors.forename: Beyrem
authors.initials: B
authors.lastname: Hamouda
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D049872:Bivalvia / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D055598:Chemical Phenomena; D004784:Environmental Monitoring; D005260:Female; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000633:toxicity*; D018570:Risk Assessment; D013482:Superoxide Dismutase / Q000378:metabolism; D014416:Tunisia; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: C000605410:2-(4-methoxyphenyl)-5,6-trimethylene-4H-1,3,2-oxathiaphosphorine-2-sulfide; D015415:Biomarkers; D009943:Organophosphorus Compounds; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase
keywords: Biomarkers; Ecotoxicology; OMTOS; Ruditapes decussatus; Sublethal effects; Vitellogenin