title: Stressed (acute) mice display neuroimmunodysregulation and defective innate immune response against coliform infection.
abstract: We examined the impact of acute restraint stress (ARS) with(out) intraperitoneal E. coli infection on TLR4 mRNA abundance in brain and spleen, clinical signs, cytokines and oxidative loads and peritoneal E. coli growth in balb/c mice. ARS exacerbated E. coli virulence and behavioral abnormality. At different post-stress hour the pattern and intensity of TLR4 activity differed in brain and spleen. While TLR4 stimulation in spleen of E. coli-infected mice was maximal, it superseded in brain of post-stressed E. coli-infected mice. ARS and E. coli infection elicited systemic pro-inflammatory and pro-oxidant status, with defective peritoneal E. coli clearance in post-ARS mice. Continuous TLR4 activation in post-stressed mice partially disarms innate immune response, and contributes to inappropriate host-E. coli interactions and thus neuroimmune dysregulation/toxicity. The description of these observed novel effects induced by ARS will provide a basis for deeper investigations of the effects from increasingly stress-oriented rural/urban life upon neuroimmune system.
authors.affiliation: Immunology Section, Department of Pathobiology, Faculty of Veterinary Medicine and Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address: mehrzad@um.ac.ir.
authors.forename: Jalil
authors.initials: J
authors.lastname: Mehrzad
authors.affiliation: Biochemistry Section, Department of Biology, Faculty of Sciences, Payame Noor University of Mashhad, Mashhad, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Shajari
authors.affiliation: Biochemistry Section, Department of Biology, Faculty of Sciences, Payame Noor University of Mashhad, Mashhad, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saleh-Moghaddam
authors.affiliation: Biochemistry Section, Department of Biology, Faculty of Sciences, Payame Noor University of Mashhad, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sarmad-Nabavi
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007249:Inflammation / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015213:Neuroimmunomodulation; D016877:Oxidants / Q000378:metabolism; D012149:Restraint, Physical; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013315:Stress, Psychological / Q000276:immunology* / Q000523:psychology; D051197:Toll-Like Receptor 4 / Q000187:drug effects / Q000378:metabolism
chemical_list: D016877:Oxidants; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4
keywords: Brain; Neuroimmunopathology; Phagocytosis; TLR4; Virulence of coliform infection