title: Phage Display-Derived Cross-Reactive Neutralizing Antibody against Enterovirus 71 and Coxsackievirus A16.
abstract: Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the Picornaviridae family and are considered the main causative agents of hand, foot and mouth disease (HFMD). In recent decades large HFMD outbreaks caused by EV71 and CVA16 have become significant public health concerns in the Asia-Pacific region. Vaccines and antiviral drugs are unavailable to prevent EV71 and CVA16 infection. In the current study, a chimeric antibody targeting a highly conserved peptide in the EV71 VP4 protein was isolated by using a phage display technique. The antibody showed cross-neutralizing capability against EV71 and CVA16 in vitro. The results suggest that this phage display-derived antibody will have great potential as a broad neutralizing antibody against EV71 and CVA16 after affinity maturation and humanization.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunyun
authors.initials: C
authors.lastname: Sun
authors.forename: Xiangqian
authors.initials: X
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Pang
authors.forename: Sisi
authors.initials: S
authors.lastname: Shen
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
authors.forename: Burton B
authors.initials: BB
authors.lastname: Yang
authors.forename: Yuming
authors.initials: Y
authors.lastname: Huang
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000302:isolation & purification*; D003429:Cross Reactions; D004770:Enterovirus / Q000276:immunology*; D029821:Enterovirus A, Human / Q000276:immunology*; D005260:Female; D008807:Mice, Inbred BALB C; D019151:Peptide Library
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019151:Peptide Library