title: Versatile Microfluidic Platform for the Assessment of Sialic Acid Expression on Cancer Cells Using Quantum Dots with Phenylboronic Acid Tags.
abstract: This work describes a versatile microfluidic platform for evaluation of cell-surface glycan expression at the single-cell level using quantum dots (QDs) tagged with phenylboronic acid. The platform was integrated with dual microwell arrays, allowing the introduction of cells in two states using the same cell culture chamber. The simultaneous analysis of cells in the same environment minimized errors resulting from different culture conditions. As proof-of-concept, the expressions of sialic acid (SA) groups on K562 cells, with or without 3'-azido-3'-deoxythymidine (AZT) treatment, were evaluated in the same chamber. 3-Aminophenylboronic acid functionalized CdSeTe@ZnS-SiO2 QDs (APBA-QDs) were prepared as probes to recognize SA groups on K562 cells with only one-step labeling. The results showed that the expression of SA moieties on K562 cells was increased by 18% and 31% after treatment with 20 and 40 μM AZT, respectively. Performing the drug treatment and control experiments simultaneously in the same chamber significantly improved the robustness and effectiveness of the assay. The strategy presented here provides an alternative tool for glycan analysis in a sensitive, high-throughput, and effective manner.
authors.affiliation: †College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, People's Republic of China.|‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Cao
authors.affiliation: ‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Peng-Hui
authors.initials: PH
authors.lastname: Zhang
authors.affiliation: †College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, People's Republic of China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: §Petrochemical Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: E S
authors.initials: ES
authors.lastname: Abdel-Halim
authors.affiliation: ‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001897:Boronic Acids / Q000493:pharmacokinetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D020014:K562 Cells; D056656:Lab-On-A-Chip Devices; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D057054:Molecular Imaging / Q000295:instrumentation; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D011134:Polysaccharides / Q000378:metabolism*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D001897:Boronic Acids; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid; C010686:benzeneboronic acid
keywords: K562 cells; QDs-APBA; dual microwell array; microfluidic platform; sialic acid