title: Development of reverse transcription loop mediated isothermal amplification assay for rapid detection of bluetongue viruses.
abstract: A single-step reverse transcription loop mediated isothermal amplification (RT-LAMP) assay targeting NS1 - a highly conserved gene among BTV serotypes was optimized and validated with seven serotypes: BTV-1, BTV-2, BTV-9, BTV-10, BTV-16, BTV-21 and BTV-23. The relative sensitivity of the assay was 0.3 TCID50 and no cross reactivity could be observed with foot and mouth disease, peste-des-petits-ruminants, goatpox, sheeppox and orf viruses. The established assay was also assessed by screening of clinical samples and the result is comparable with conventional RT-PCR. The RT-LAMP assay described here could be an additional tool to the existing assays for diagnosis/surveillance of BTV.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Sreekala S
authors.initials: SS
authors.lastname: Mohandas
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India. Electronic address: drchelva@gmail.com.
authors.forename: Dhanavelu
authors.initials: D
authors.lastname: Muthuchelvan
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Awadh Bihari
authors.initials: AB
authors.lastname: Pandey
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Sanchay Kumar
authors.initials: SK
authors.lastname: Biswas
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Karam
authors.initials: K
authors.lastname: Chand
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Gnanavel
authors.initials: G
authors.lastname: Venkatesan
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Dheeraj
authors.initials: D
authors.lastname: Choudhary
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
authors.forename: Muthannan Andavar
authors.initials: MA
authors.lastname: Ramakrishnan
authors.affiliation: Indian Veterinary Research Institute, Kolkata 700 037 West Bengal, India.
authors.forename: Bimalendu
authors.initials: B
authors.lastname: Mondal
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000175:diagnosis*; D001820:Bluetongue virus / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012418:Ruminants; D012680:Sensitivity and Specificity; D013997:Time Factors
keywords: Bluetongue; Isothermal amplification; RT-LAMP; Ruminants