title: Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis.
abstract: Although there is no evidence for a role of anti-MOG antibodies in adult MS, no information on B lymphocytes with MOG-committed BCR is available. We report here on the frequency of anti-MOG B cells forming rosettes with polystyrene beads (BBR) covalently bound to the extracellular domain of rhMOG in 38 relapsing-remitting patients (RRMS) and 50 healthy individuals (HI). We show a substantial proportion of circulating anti-MOG-BBR in both RRMS and HI. Strikingly, MOG-specific B cells frequencies were lower in MS than in HI. Anti-MOG antibodies measured by a cell-based assay were not different between MS patients and controls, suggesting a specific alteration of anti-MOG B cells in MS. Although anti-MOG-BBR were higher in CNS fluid than in blood, no difference was observed between MS and controls. Lower frequency of MOG-BBR in MS was not explained by an increased apoptosis, but a trend for lower proliferative capacity was noted. Despite an efficient B cell transmigration across brain derived endothelial cells, total and anti-MOG B cells transmigration was similar between MS and HI. The striking alteration in MOG-specific B cells, independent of anti-MOG antibody titers, challenges our view on the role of MOG-specific B cells in MS.
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Elong Ngono
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Service de Neurologie, CHU de Nantes, 44093 Nantes, France.
authors.forename: Maud
authors.initials: M
authors.lastname: Lepetit
authors.affiliation: Clinical Department of Neurology, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Markus
authors.identifier: 0000-0003-2817-1402
authors.initials: M
authors.lastname: Reindl
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Garcia
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Flora
authors.initials: F
authors.lastname: Guillot
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France.
authors.forename: Athénaïs
authors.initials: A
authors.lastname: Genty
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Chesneau
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Salou
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Service de Neurologie, CHU de Nantes, 44093 Nantes, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Michel
authors.affiliation: INSERM, CIC 004, 44093 Nantes, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Lefrere
authors.affiliation: Clinical Department of Neurology, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schanda
authors.affiliation: Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Berthe-Marie
authors.initials: BM
authors.lastname: Imbert-Marcille
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Degauque
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Nicot
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Brouard
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France ; Service de Neurologie, CHU de Nantes, 44093 Nantes, France.
authors.forename: David-Axel
authors.initials: DA
authors.lastname: Laplaud
authors.affiliation: INSERM, UMR1064, 44093 Nantes, France ; Faculté de Médecine, Université de Nantes, 44035 Nantes, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Soulillou
mesh_terms: D000328:Adult; D000906:Antibodies / Q000276:immunology; D017209:Apoptosis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology*
chemical_list: D000906:Antibodies; D063308:Myelin-Oligodendrocyte Glycoprotein