title: Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients.
abstract: OBJECTIVE
  To investigate whether the proportions of acetyl-histone-positive hepatocytes could be used as markers of deteriorating liver function.
  
  
  METHODS
  In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically.
  
  
  RESULTS
  The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05).
  
  
  CONCLUSIONS
  The proportions of acetyl-histone-positive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xia
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Bao
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Shi
authors.affiliation: Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Bu
mesh_terms: D000107:Acetylation; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D002648:Child; D005260:Female; D022781:Hepatocytes / Q000737:chemistry* / Q000473:pathology; D006657:Histones / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D008103:Liver Cirrhosis / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D012720:Severity of Illness Index; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D006657:Histones
keywords: Cirrhosis; Histone acetylation; Liver function; Prognosis