title: Fluctuation of Bacteria on Bleb Surface After Trabeculectomy With Adjunctive Mitomycin C.
abstract: To determine whether the bacterial and floral patterns on the bleb surface are affected by the season in eyes that had undergone trabeculectomy with adjunctive mitomycin C.
  
  
  
  Forty-four glaucoma patients who had an avascular or a hypovascular cystic filtering bleb were studied. Swabs of the bleb surface were taken 4 times in 1 year. The samples were cultured, and all organisms isolated were identified and tested for antibiotic sensitivity and resistance.
  
  
  
  Of the 176 specimens, 48 tested positive in cultures. Out of the 44 glaucoma eyes that had undergone trabeculectomy, 30 (68.2%) eyes were culture positive. A total of 58 strains were isolated. The organisms isolated were 22 strains of Staphylococcus epidermidis, 21 strains of Propionibacterium acnes, 8 strains of Corynebacterium sp., 5 strains of Staphylococcus sp., and 1 strain of both Neisseria sp., and Candida parapsilosis. Fifteen eyes had a positive culture ≥2 times, and in 10 of these eyes, the same strain was isolated. There was no resistance to vancomycin by S. epidermidis, P. acnes, and Corynebacterium sp. All of the isolates of S.epidermidis were sensitive to minocycline and amikacin. The rate of bacterial detection in the spring was 13.6%, summer was 20.5%, autumn was 45.5%, and winter was 29.5%. The increase in the incidence of bacterial presence during autumn was significant (P=0.006; the Fisher exact probability test).
  
  
  
  It is not rare to detect the bacterial organisms on the bleb surface in glaucomatous eyes that had undergone trabeculectomy. The prevalence varied with the season and was highest in the autumn.
authors.affiliation: Department of Ophthalmology, Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Takahashi
authors.forename: Akira
authors.initials: A
authors.lastname: Sawada
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Katada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000477:Alkylating Agents / Q000008:administration & dosage*; D001419:Bacteria / Q000302:isolation & purification; D003131:Combined Modality Therapy; D003228:Conjunctiva / Q000382:microbiology*; D005260:Female; D005658:Fungi / Q000302:isolation & purification; D005901:Glaucoma / Q000503:physiopathology / Q000601:surgery*; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016685:Mitomycin / Q000008:administration & dosage*; D012621:Seasons; D013524:Surgical Flaps / Q000382:microbiology*; D014130:Trabeculectomy / Q000379:methods*
chemical_list: D000477:Alkylating Agents; D016685:Mitomycin