title: Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb.
abstract: OBJECTIVE
  To investigate the anti-inflammatory and antioxidant effect of curcumin on lung lesion induced by intestinal ischemia reperfusion injury (IIR).
  
  
  METHODS
  Rats were divided into four groups: sham, intestinal IIR (IIR), 1 mg/kg of curcumin treatment group (1 mg/kg), and 5 mg/kg of curcumin treatment group (5 mg/kg). Curcumin was given respectively (1 mg/kg and 5 mg/kg) following the above doses. IIR was produced by 1 h of intestinal ischemia followed by 2 h of reperfusion. Rats were sacrificed at the end of reperfusion and lung tissues were collected for biochemical and histopathological examination in 4 groups. Lung tissues histology and bronchoalveolar lavage fluid (BALF) protein were assayed. Serum IL-6, lung superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured. The expression level of NF-κB and ICAM-1 (including immunohistochemical analysis and western blot analysis) were also measured.
  
  
  RESULTS
  Lung tissue injury induced by IIR was obviously observed through pathology and BALF protein. MPO activity, IL-6 level and ICAM-1 expression were significantly increased with the elevation of NF-κB, simultaneously, SOD activity was decreased. With Treatment of curcumin, pathology and BALF protein of lung tissue were improved clearly. Inflammatory indexes (MPO activity, IL-6 level and ICAM-1) were improved and antioxidant index (SOD activity) was enhanced paralleled with NF-κB.
  
  
  CONCLUSIONS
  Using curcumin effectively prevented IIR-induced lung injury. Anti-inflammatory and antioxidant effects of curcumin could be observed by inhibiting the pathway of NF-κB.
authors.affiliation: Department of General Surgery, The Third People's Hospital of Dalian Dalian 116033, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Pharmacology, Dalian Medical University Dalian 116044, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of General Surgery, Liaoning Cancer Hospital and Institute Shenyang 110042, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Dalian Medical University Dalian 116044, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Pharmacology, Dalian Medical University Dalian 116044, China.
authors.forename: Huizhu
authors.initials: H
authors.lastname: Shao
authors.affiliation: Department of General Surgery, The Second Hospital of Dalian Medical University Dalian 116023, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Tian
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000473:pathology; D008168:Lung / Q000201:enzymology / Q000473:pathology; D008297:Male; D023421:Models, Animal; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D016328:NF-kappa B; D003474:Curcumin
keywords: Curcumin; NF-κB; intestinal ischemia reperfusion; lung injury