title: High co-expression of Sp1 and HER-2 is correlated with poor prognosis of gastric cancer patients.
abstract: The aim of this study was to investigate co-expression of HER-2 and Sp1 in gastric cancer (GC) so as to determine whether these two proteins may be correlated with poor prognosis of GC patients. We examined the HER-2 overexpression and amplification and expression levels of Sp1 in 227 GC patients using immune-histochemical staining and fluorescence in situ hybridization. Data on clinicopathological features and relevant prognostic factors in these patients were analyzed. Of the 227 gastric cancer samples, 11.89% were positive for HER-2 overexpression/amplification under the new scoring system, and the frequency of negative, weak positive and strong positive expression of Sp1 was 14.98%, 48.01% and 37.0% respectively. No statistically positive correlation was observed between the expression levels of HER-2 and Sp1 in GC tissues. HER-2 overexpression was closely correlated to the Lauren type, degree of differentiation, tumor size and lymph node metastasis, and Sp1 as well (P < 0.05). Overexpression of HER-2 and Sp1 predicted poor survival in univariate analysis as well as in a Cox proportional hazards model.
authors.affiliation: Department of Oncology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, People's Republic of China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 200080, People's Republic of China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Oncology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, People's Republic of China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 200080, People's Republic of China.
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of Oncology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, People's Republic of China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 200080, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, People's Republic of China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 200080, People's Republic of China.
authors.forename: Jiujie
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Oncology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, People's Republic of China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 200080, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oncology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, People's Republic of China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 200080, People's Republic of China. Electronic address: yzwlw@hotmail.com.
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism*; D009367:Neoplasm Staging; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D015996:Survival Rate; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human; C508053:ERBB2 protein, human
keywords: Gastric cancer; HER-2; Immunohistochemistry; Prognosis; Sp1