title: Detection of mycobacterial DNA by a specific and simple lateral flow assay incorporating cadmium selenide quantum dots.
abstract: Cadmium selenide quantum dots have been incorporated to a lateral flow assay for the specific and very simple detection of different mycobacterial DNA targets within only a few minutes, bypassing the complexity of conventional DNA hybridization assays. The method extends our previous work on protein detection using an identical procedure.
authors.affiliation: Biotecgen SRL, Via Leuca 174, 73100 Lecce, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Cimaglia
authors.affiliation: VISAVET Health Surveillance Centre, Complutense University of Madrid, Av. Puerta de Hierro s/n 28040 Madrid, Spain.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Liandris
authors.affiliation: Laboratory of Biology, School of Medicine, Department of Basic Medical Science, Kapodestrian University of Athens, 11527 Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Gazouli
authors.affiliation: Dipartamento di Scienze Biomediche, Sezione di Microbiologia Clinica e Sperimentale, Sassary, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sechi
authors.affiliation: Biotecgen SRL, Via Leuca 174, 73100 Lecce, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Chiesa
authors.affiliation: Biotecgen SRL, Via Leuca 174, 73100 Lecce, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: De Lorenzis
authors.affiliation: Laboratory of Anatomy and Physiology of Farm Animals, Faculty of Animal Health and Aquaculture, Agricultural University of Athens, 11855 Athens, Greece.
authors.forename: Margarita
authors.initials: M
authors.lastname: Andreadou
authors.affiliation: Laboratory of Anatomy and Physiology of Farm Animals, Faculty of Animal Health and Aquaculture, Agricultural University of Athens, 11855 Athens, Greece.
authors.forename: Styliani
authors.initials: S
authors.lastname: Taka
authors.affiliation: Laboratory of Anatomy and Physiology of Farm Animals, Faculty of Animal Health and Aquaculture, Agricultural University of Athens, 11855 Athens, Greece.
authors.forename: Antonia
authors.initials: A
authors.lastname: Mataragka
authors.affiliation: Laboratory of Anatomy and Physiology of Farm Animals, Faculty of Animal Health and Aquaculture, Agricultural University of Athens, 11855 Athens, Greece. Electronic address: ikonomop@aua.gr.
authors.forename: John
authors.initials: J
authors.lastname: Ikonomopoulos
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D045663:Quantum Dots / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D004269:DNA, Bacterial; D018036:Selenium Compounds; C058667:cadmium selenide
keywords: Cadmium selenide; Mycobacteria; Nanotechnology; Rapid detection