title: [Alkaloids from Macleaya cordata and their cytotoxicity assay].
abstract: OBJECTIVE
  To study the alkaloids of Macleaya cordata and their anti-tumor activities.
  
  
  METHODS
  Alcohol and liquid-liquid extraction were used methods were used to extract the alkaloids constituents, and silica gel, reverse-phase octadecylsilyl (ODS), sephadex LH-20 chromatographic methods and HPLC were applied to isolate and purify compounds. MS, NMR spectroscopic methods were used to determine their structures. Furthermore, the cytotoxicity of these chemical components for MCF-7 and SF-268 cell lines was measured by MTT method.
  
  
  RESULTS
  Twelve alkaloids were isolated from the fruits of M. cordata, and their structures were identified as: maclekarpine E (1), 6-acetonyldihyrochelerythrine (2), cavidilinine (3), 6-acetonyldihyrosanguinnarine (4), O-methylzanthoxyline (5), 6-methoxy-dihydrosanguinarine (6), spallidamine (7), 6-hydroxyldihydrochelerythrine (8), arnotianamida (9), dihydrosanguinarine (10), protopine (11), and cryptopine (12).
  
  
  CONCLUSIONS
  Compounds 1, 3, 7-9 were isolated from M. cordata for the first time, and compound 5 is a new natural product. The results of cytotoxic assay indicated that compound 6 showed strong cytotoxicity against MCF-7 and SF-268 cell lines with IC50 values of 0.61 μmol · L(-1) and 0.54 μmol · L(-1), respectively.
authors.forename: Hui-liang
authors.initials: HL
authors.lastname: Zou
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Li
authors.forename: Shao-fu
authors.initials: SF
authors.lastname: Yu
authors.forename: Miao
authors.initials: M
authors.lastname: Cheng
authors.forename: Xing-dong
authors.initials: XD
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bai-lian
authors.initials: BL
authors.lastname: Liu
authors.forename: Guang-xiong
authors.initials: GX
authors.lastname: Zhou
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D006801:Humans; D028101:Papaveraceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic