title: Compound Transfer by Acoustic Droplet Ejection Promotes Quality and Efficiency in Ultra-High-Throughput Screening Campaigns.
abstract: Acoustic droplet ejection (ADE) as a means of transferring library compounds has had a dramatic impact on the way in which high-throughput screening campaigns are conducted in many laboratories. Two Labcyte Echo ADE liquid handlers form the core of the compound transfer operation in our 1536-well based ultra-high-throughput screening (uHTS) system. Use of these instruments has promoted flexibility in compound formatting in addition to minimizing waste and eliminating compound carryover. We describe the use of ADE for the generation of assay-ready plates for primary screening as well as for follow-up dose-response evaluations. Custom software has enabled us to harness the information generated by the ADE instrumentation. Compound transfer via ADE also contributes to the screening process outside of the uHTS system. A second fully automated ADE-based system has been used to augment the capacity of the uHTS system as well as to permit efficient use of previously picked compound aliquots for secondary assay evaluations. Essential to the utility of ADE in the high-throughput screening process is the high quality of the resulting data. Examples of data generated at various stages of high-throughput screening campaigns are provided. Advantages and disadvantages of the use of ADE in high-throughput screening are discussed.
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Dawes
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Turincio
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Steven W
authors.initials: SW
authors.lastname: Jones
authors.affiliation: Department of Biological Technologies, Genentech, South San Francisco, CA, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Rodriguez
authors.affiliation: Gadgitech Ltd, London, UK.
authors.forename: Dhireshan
authors.initials: D
authors.lastname: Gadiagellan
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Thana
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Clark
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Gustafson
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Orren
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Marya
authors.initials: M
authors.lastname: Liimatta
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Gross
authors.affiliation: Department of Structural Biology, Genentech, South San Francisco, CA, USA.
authors.forename: Till
authors.initials: T
authors.lastname: Maurer
authors.affiliation: Department of Biochemical & Cellular Pharmacology, Genentech, South San Francisco, CA, USA beresini@gene.com.
authors.forename: Maureen H
authors.initials: MH
authors.lastname: Beresini
mesh_terms: D000162:Acoustics; D020811:Biomedical Technology / Q000295:instrumentation / Q000379:methods*; D003627:Data Interpretation, Statistical; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D054852:Small Molecule Libraries; D012984:Software; D012996:Solutions
chemical_list: D054852:Small Molecule Libraries; D012996:Solutions
keywords: acoustic droplet ejection; assay-ready plates; high-throughput screening