title: Single-cell magnetic imaging using a quantum diamond microscope.
abstract: We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (∼1 mm(2)) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.
authors.affiliation: Harvard-Smithsonian Center for Astrophysics, Cambridge, Massachusetts, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Glenn
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.|Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.|Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.|Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: R
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: A
authors.initials: A
authors.lastname: Yacoby
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Lukin
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.affiliation: Harvard-Smithsonian Center for Astrophysics, Cambridge, Massachusetts, USA.|Department of Physics, Harvard University, Cambridge, Massachusetts, USA.|Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Walsworth
authors.affiliation: Quantum Diamond Technologies, Inc., Somerville, MA, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Connolly
mesh_terms: D000906:Antibodies / Q000737:chemistry; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods; D018130:Diamond; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D061986:MCF-7 Cells; D060328:Magnetic Phenomena; D008280:Magnetics; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods; D009584:Nitrogen / Q000737:chemistry; D011789:Quantum Theory; D059010:Single-Cell Analysis
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D018130:Diamond; D009584:Nitrogen