title: New features of triacylglycerol biosynthetic pathways of peanut seeds in early developmental stages.
abstract: The peanut (Arachis hypogaea L.) is one of the three most important oil crops in the world due to its high average oil content (50 %). To reveal the biosynthetic pathways of seed oil in the early developmental stages of peanut pods with the goal of improving the oil quality, we presented a method combining deep sequencing analysis of the peanut pod transcriptome and quantitative real-time PCR (RT-PCR) verification of seed oil-related genes. From the sequencing data, approximately 1500 lipid metabolism-associated Unigenes were identified. The RT-PCR results quantified the different expression patterns of these triacylglycerol (TAG) synthesis-related genes in the early developmental stages of peanut pods. Based on these results and analysis, we proposed a novel construct of the metabolic pathways involved in the biosynthesis of TAG, including the Kennedy pathway, acyl-CoA-independent pathway and proposed monoacylglycerol pathway. It showed that the biosynthetic pathways of TAG in the early developmental stages of peanut pods were much more complicated than a simple, unidirectional, linear pathway.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Road No.61, Tai'an, Shandong, 271000, China. yml1227@126.com.
authors.forename: Mingli
authors.initials: M
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University, Tai'an, Shandong, 271000, China. liufz@sdau.edu.cn.
authors.forename: Fengzhen
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Road No.61, Tai'an, Shandong, 271000, China. 864878248@qq.com.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Road No.61, Tai'an, Shandong, 271000, China. 873126275@qq.com.
authors.forename: Meihong
authors.initials: M
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Road No.61, Tai'an, Shandong, 271000, China. 562329679@qq.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Road No.61, Tai'an, Shandong, 271000, China. l1999xz@163.com.
authors.forename: Xinzheng
authors.initials: X
authors.lastname: Li
mesh_terms: D010367:Arachis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D012639:Seeds / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014280:Triglycerides
keywords: Arachis hypogaea L.; Seed oil; Triacylglycerol (TAG)