title: Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue.
abstract: Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Sun
authors.affiliation: Intramural Research Program, National Institute on Drug Abuse, 251 Bayview Boulevard, Baltimore, Maryland 21224, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Richie
authors.affiliation: Intramural Research Program, National Institute on Drug Abuse, 251 Bayview Boulevard, Baltimore, Maryland 21224, USA.
authors.forename: Brandon K
authors.initials: BK
authors.lastname: Harvey
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Betzig
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA.
authors.forename: Na
authors.initials: N
authors.lastname: Ji
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology; D059907:Functional Neuroimaging; D000069416:Intravital Microscopy / Q000379:methods*; D008027:Light; D051379:Mice; D059906:Neuroimaging; D061848:Optical Imaging / Q000379:methods*