title: MicroRNA-139-5p inhibits cell proliferation and invasion by targeting insulin-like growth factor 1 receptor in human non-small cell lung cancer.
abstract: BACKGROUND
  Increasing evidence suggested that microRNAs (miRNAs) play a critical role in tumorigenesis. Decreased expression of miRNA-139-5p has been observed in various types of cancers. However, the biological function of miRNA-139-5p in non-small cell lung cancer (NSCLC) is still largely unknown.
  
  
  METHODS
  Quantitative real-time PCR (qRT-PCR) was used to explore the expression level of miRNA-139-5p in NSCLC tissues and cell lines. Then, we investigated the role of miRNA-139-5p to determine its potential roles on lung cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was performed to confirm the target gene of miRNA-139-5p and the results were validated in renal cancer cells.
  
  
  RESULTS
  miRNA-139-5p was significantly decreased in NSCLC tissues and cell lines. Over-expression of miRNA-139-5p could inhibit lung cancer cell proliferation, migration, and invasion in vitro. Furthermore, we identified insulin-like growth factor 1 receptor (IGF1R) as a target of miR-139-5p and miR-139-5p function as a tumor suppressor via targeting IGF1R in NSCLC.
  
  
  CONCLUSIONS
  Our results indicated that miR-139-5p acts as a tumor suppressor in NSCLC partially via down-regulating IGF1R expression.
authors.affiliation: Medical Oncology, Nanjing Medical University Affiliated Wuxi Second Hospital Wuxi 214002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Medical Oncology, Nanjing Medical University Affiliated Wuxi Second Hospital Wuxi 214002, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Hang
authors.affiliation: Medical Oncology, Nanjing Medical University Affiliated Wuxi Second Hospital Wuxi 214002, China.
authors.forename: Chen-Ye
authors.initials: CY
authors.lastname: Yuan
authors.affiliation: Medical Oncology, Nanjing Medical University Affiliated Wuxi Second Hospital Wuxi 214002, China.
authors.forename: Fu-Lin
authors.initials: FL
authors.lastname: Wu
authors.affiliation: Medical Oncology, Nanjing Medical University Affiliated Wuxi Second Hospital Wuxi 214002, China.
authors.forename: Shu-Bo
authors.initials: SB
authors.lastname: Chen
authors.affiliation: Medical Oncology, Fudan University Shanghai Cancer Center Shanghai 200032, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xue
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: C555098:MIRN139 microRNA, human; D035683:MicroRNAs; D017526:Receptor, IGF Type 1
keywords: Non-small cell lung cancer; insulin-like growth factor 1 receptor; miR-139-5p