title: Organ In Vitro Culture: What Have We Learned about Early Kidney Development?
abstract: When Clifford Grobstein set out to study the inductive interaction between tissues in the developing embryo, he developed a method that remained important for the study of renal development until now. From the late 1950s on, in vitro cultivation of the metanephric kidney became a standard method. It provided an artificial environment that served as an open platform to study organogenesis. This review provides an introduction to the technique of organ culture, describes how the Grobstein assay and its variants have been used to study aspects of mesenchymal induction, and describes the search for natural and chemical inducers of the metanephric mesenchyme. The review also focuses on renal development, starting with ectopic budding of the ureteric bud, ureteric bud branching, and the generation of the nephron and presents the search for stem cells and renal progenitor cells that contribute to specific structures and tissues during renal development. It also presents the current use of Grobstein assay and its modifications in regenerative medicine and tissue engineering today. Together, this review highlights the importance of ex vivo kidney studies as a way to acquire new knowledge, which in the future can and will be implemented for developmental biology and regenerative medicine applications.
authors.affiliation: Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Oulu University, 90220 Oulu, Finland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rak-Raszewska
authors.affiliation: Renal Regeneration Laboratory, VAGLAHS at Sepulveda, North Hills, CA 91343, USA ; David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Peter V
authors.initials: PV
authors.lastname: Hauser
authors.affiliation: Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Oulu University, 90220 Oulu, Finland.
authors.forename: Seppo
authors.initials: S
authors.lastname: Vainio