title: Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis.
abstract: Recent studies suggest that SATB1 is a promising therapeutic target for prostate cancer. To develop novel SATB1-based therapeutic agents for prostate cancer, in this study, we aimed to construct ZD55-SATB1, an oncolytic adenovirus ZD55 carrying shRNA targeting SATB1, and investigate its effects on the inhibition of prostate cancer growth and metastasis. ZD55-SATB1 was constructed and used to infect human prostate cancer cell lines DU145 and LNCaP. The inhibitory effect of ZD55-SATB1 on SATB1 expression was evaluated by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. The cytotoxicity of ZD55-SATB1 was detected by MTT assay. Cell invasion was detected by Matrigel invasion assay. The in vivo antitumor activities of ZD55-SATB1 were evaluated in xenograft mouse model. We found that ZD55-SATB1 selectively replicated and significantly reduced SATB1 expression in DU145 and LNCaP cells. ZD55-SATB1 effectively inhibited the viability and invasion of DU145 and LNCaP cells in vitro and inhibited prostate cancer growth and metastasis in xenograft nude mice. In conclusion, replicative oncolytic adenovirus armed with SATB1 shRNA exhibits effective antitumor effect in human prostate cancer. Our study provides the basis for the development of ZD55-SATB1 for the treatment of prostate cancer.
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.|Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Mao
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, 221002, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Dong-rong
authors.initials: DR
authors.lastname: Yang
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Bo-xin
authors.initials: BX
authors.lastname: Xue
authors.affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. maolijunxz@163.com.
authors.forename: Yu-xi
authors.initials: YX
authors.lastname: Shan
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, 221002, China. zhengjunnian@163.com.
authors.forename: Jun-nian
authors.initials: JN
authors.lastname: Zheng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D050504:Oncolytic Viruses / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D036961:Matrix Attachment Region Binding Proteins; D034741:RNA, Small Interfering; C076189:SATB1 protein, human
keywords: Oncolytic adenovirus; Prostate cancer; SATB1; shRNA