title: Screening of Propionibacterium spp. for potential probiotic properties.
abstract: The main topic of this paper is the evaluation of adhesion of propionibacteria to IPEC-J2 cells and the survival at pH 2.5 and with 0.3% bile salts added, bioactivity towards pathogens and antibiotic resistance of Propionibacterium freudenreichii subsp. shermanii, Propionibacterium jensenii, Propionibacterium acidipropionici and Propionibacterium thoenii. Adhesion to IPEC-J2 cell lines was ca. 25-35% and significantly increased with CaCl2. Moreover, propionibacteria showed a reduction of cell count of ca. 0.5% at pH 2.5 after 3 h, whereas cell count increased after 24 h with bile salts; finally, they significantly inhibited Escherichia coli O157:H7.
authors.affiliation: Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Campaniello
authors.affiliation: Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bevilacqua
authors.affiliation: Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Sinigaglia
authors.affiliation: Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy. Electronic address: clelia.altieri@unifg.it.
authors.forename: Clelia
authors.initials: C
authors.lastname: Altieri
mesh_terms: D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion; D001647:Bile Acids and Salts / Q000378:metabolism; D002460:Cell Line; D024881:Drug Resistance, Bacterial; D004847:Epithelial Cells / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability / Q000187:drug effects; D019936:Probiotics; D011424:Propionibacterium / Q000187:drug effects / Q000502:physiology*; D013552:Swine
chemical_list: D001647:Bile Acids and Salts
keywords: Adhesion; IPEC-J2 cells; Propionibacteria; Selection criteria