title: Insight into the Migration Routes of Plutella xylostella in China Using mtCOI and ISSR Markers.
abstract: The larvae of the diamondback moth, Plutella xylostella, cause major economic losses to cruciferous crops, including cabbage, which is an important vegetable crop in China. In this study, we used the mitochondrial COI gene and 11 ISSR markers to characterize the genetic structure and seasonal migration routes of 23 P. xylostella populations in China. Both the mitochondrial and nuclear markers revealed high haplotype diversity and gene flow among the populations, although some degree of genetic isolation was evident between the populations of Hainan Island and other sampling sites. The dominant haplotypes, LX1 and LX2, differed significantly from all other haplotypes both in terms of the number of individuals with those haplotypes and their distributions. Haplotypes that were shared among populations revealed that P. xylostella migrates from the lower reaches of the Yangtze River to northern China and then to northeastern China. Our results also revealed another potential migration route for P. xylostella, i.e., from southwestern China to both northwestern and southern China.
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Tian
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Baoyun
authors.initials: B
authors.lastname: Xu
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xie
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Wu
mesh_terms: D025041:Animal Migration / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D003576:Electron Transport Complex IV / Q000235:genetics; D051456:Gene Flow / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D003576:Electron Transport Complex IV