title: Species Authentication of Common Meat Based on PCR Analysis of the Mitochondrial COI Gene.
abstract: Adulteration of meat products and costly animal-derived commodities with their inferior/cheaper counterparts is a grievous global problem. Species authentication is still technical challenging, especially to those deep processed products. The present study described the design of seven sets of species-specific primer based on a high heterozygous region of mitochondrial cytochrome c oxidase subunit I (COI) gene. These primers were proven to have high species specificity and no cross-reactions and unexpected products to different DNA source. Multiplex PCR assay was achieved for rapid and economical identification of four commonly consumed meats (pork, beef, chicken, and mutton). The conventional PCR assay was sensitive down to 0.001 ng of DNA template in the reactant. The developed method was also powerful in detecting as low as 0.1-mg adulterated pork (0.05 % in wt/wt) in an artificial counterfeited mutton. Validation test showed that the assay is specific, reproducible, and robust in commercial deep processed meats, leatherware, and feather commodities. This proposed method will be greatly beneficial to the consumers, food industry, leather, and feather commodity manufacture.
authors.affiliation: Department of Biochemistry, College of Basic Medical Sciences, Nanchang University, 330006,, No 461, Bayi Avenue, Nanchang, Jiangxi Province, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Dai
authors.forename: Jiao
authors.initials: J
authors.lastname: Qiao
authors.forename: Siran
authors.initials: S
authors.lastname: Yang
authors.forename: Shen
authors.initials: S
authors.lastname: Hu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zuo
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhu
authors.forename: Chunhong
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics*; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D008460:Meat / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D003576:Electron Transport Complex IV