title: Prevention of Polyglycolic Acid-Induced Peritoneal Adhesions Using Alginate in a Rat Model.
abstract: Postoperative intra-abdominal or intrathoracic adhesions sometimes cause significant morbidity. We have designed three types of alginate-based treatments using strongly cross-linked (SL), weakly cross-linked (WL), and non-cross-linked (NL) alginate with calcium gluconate. In rat experiments, we compared the antiadhesive effects of the three types of alginate-based treatments, fibrin glue treatment (a standard treatment), and no treatment against adhesions caused by polyglycolic acid (PGA) mesh (PGA-induced adhesions). The antiadhesive materials were set on the PGA sheet fixed on the parietal peritoneum of the abdomen. Fifty-six days later, the adhesions were evaluated macroscopically by the adhesion scores and microscopically by hematoxylin-eosin staining and immunostaining. We also tested the fibroblast growth on the surface of the antiadhesive materials in vitro. The antiadhesive effects of WL and NL were superior to the no treatment and fibrin glue treatment. A microscopic evaluation confirmed that the PGA sheet was covered by a peritoneal layer constructed of well-differentiated mesothelial cells, and the inflammation was most improved in the NL and WL. The fibroblast growth was inhibited most on the surfaces of the NL and WL. These results suggest that either the WL or NL treatments are suitable for preventing PGA-induced adhesions compared to SL or the conventional treatment.
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan ; Graduate School of Medicine for the Master's Course, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Matoba
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Hashimoto
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tanzawa
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Orikasa
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Junki
authors.initials: J
authors.lastname: Ikeda
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Yoshizumi
authors.initials: Y
authors.lastname: Iwame
authors.affiliation: Kusatsu General Hospital, 1660 Yabase-cho, Kusatsu, Shiga 525-8585, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ozamoto
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Rie
authors.identifier: 0000-0002-8331-9513
authors.initials: R
authors.lastname: Abe
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Chiko
authors.initials: C
authors.lastname: Yoshida
authors.affiliation: Department of Economics, Doshisha University, Imadegawa-Karasuma, Kamigyo-ku, Kyoto 602-8580, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Torii
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Takamori
authors.affiliation: Biomedical Material Research Center, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0321, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Morita
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsujimoto
authors.affiliation: Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Akeo
authors.initials: A
authors.lastname: Hagiwara
mesh_terms: D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D002125:Calcium Gluconate / Q000008:administration & dosage*; D002448:Cell Adhesion / Q000187:drug effects*; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage; D005347:Fibroblasts / Q000187:drug effects; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D006801:Humans; D010537:Peritoneum / Q000187:drug effects / Q000473:pathology; D011100:Polyglycolic Acid / Q000008:administration & dosage; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control; D051381:Rats; D013526:Surgical Mesh; D000267:Tissue Adhesions / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000464:Alginates; D015718:Fibrin Tissue Adhesive; D006603:Hexuronic Acids; D011100:Polyglycolic Acid; D020723:Glucuronic Acid; D002125:Calcium Gluconate