title: Population data of the GlobalFiler(®) Express loci in South Portuguese population.
abstract: Allele frequencies and other relevant forensic parameters for 21 loci studied with GlobalFiler(®) Express amplification kit (Life Technologies) were calculated in a population of individuals residing in the south of Portugal. Blood stain samples were obtained from a total of 502 unrelated individuals involved in paternity testing casework and directly PCR amplified with GlobalFiler(®) Express following manufacturer's instructions. This kit comprises all the loci included in the extended European Standard Set (ESS) and in the Combined DNA Index System (CODIS), besides the very polymorphic D2S441, D19S433, and SE33. In our laboratory this is used as a screening tool to solve complex cases, as fatherless paternity tests or to help in paternity investigations where there is the need to study additional genetic markers. These studies are necessary to calculate statistical forensic parameters, such as power of discrimination or as power of exclusion. Statistical parameters including heterozigosity, homozigosity and combined power of exclusion were estimated.
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; University of Lisbon, Faculty of Sciences, Portugal. Electronic address: carina.almeid@gmail.com.
authors.forename: Carina
authors.initials: C
authors.lastname: Almeida
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal. Electronic address: tribeiro@dlinml.mj.pt.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ribeiro
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; University of Lisbon, Faculty of Sciences, Portugal. Electronic address: aroliveira@fc.ul.pt.
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Oliveira
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal. Electronic address: mariajoao.porto@dcinml.mj.pt.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Porto
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal; University of Lisbon, Faculty of Medicine, Portugal. Electronic address: jsantos@dlinml.mj.pt.
authors.forename: Jorge
authors.initials: J
authors.lastname: Costa Santos
authors.affiliation: University of Lisbon, Faculty of Science, Portugal; CESAM-Centre for Environmental and Marine Studies, Lisboa, Portugal. Electronic address: dmdias@fc.ul.pt.
authors.forename: Deodália
authors.initials: D
authors.lastname: Dias
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; University of Lisbon, Faculty of Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal; CESAM-Centre for Environmental and Marine Studies, Lisboa, Portugal. Electronic address: pdario@dlinml.mj.pt.
authors.forename: Paulo
authors.initials: P
authors.lastname: Dario
mesh_terms: D004247:DNA / Q000235:genetics; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D011174:Portugal
chemical_list: D004247:DNA
keywords: Globalfiler(®) express; Population genetics; Portugal