title: Predictive Levels of CD24 in Peripheral Blood Leukocytes for the Early Detection of Colorectal Adenomas and Adenocarcinomas.
abstract: CD24 is expressed in 90% of colorectal adenomas and adenocarcinomas. Colorectal cancer (CRC) can be mostly prevented but average risk population screening by stool testing or colonoscopy faces many hurdles. Blood testing is clinically needed. We aimed to evaluate the utility of CD24 expression in peripheral blood leukocytes (PBLs). Two independent case studies were conducted in eligible individuals undergoing colonoscopy. Protein extracted from PBLs was subjected to immunoblotting using anti-CD24 monoclonal antibodies. CD24 sensitivity and specificity were determined using receiver operating characteristic (ROC) analysis. Initially, 150 subjects were examined: 63 had CRC, 19 had adenomas, and 68 had normal colonoscopies. The sensitivity and specificity of CD24 for distinguishing CRC from normal subjects were 70.5% (95% CI, 54.8-83.2%) and 83.8% (95% CI, 74.6-92.7%) and for adenomas 84.2% (95% CI, 60.4-96.4%) and 73.5% (95% CI, 61.4-83.5%), respectively. In the second trial (n = 149), a similar specificity but higher sensitivity was achieved: 80.0% (95% CI, 63.1-91.6%) for CRC and 89.2% (95% CI, 74.6-97%) for adenomas. A simple noninvasive blood test evaluating CD24 levels has high sensitivity and specificity for detecting colorectal adenomas and cancer in patients undergoing colonoscopy at an urban medical center. Larger multicenter studies are warranted to establish the potential of this promising test.
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel ; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kraus
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel ; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Shiran
authors.initials: S
authors.lastname: Shapira
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Dina
authors.initials: D
authors.lastname: Kazanov
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Inna
authors.initials: I
authors.lastname: Naumov
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel ; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel ; Department of Gastroenterology, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Menachem
authors.initials: M
authors.lastname: Moshkowitz
authors.affiliation: Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel ; Department of Gastroenterology, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Erwin
authors.initials: E
authors.lastname: Santo
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Lior
authors.initials: L
authors.lastname: Galazan
authors.affiliation: Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel ; Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Ravit
authors.identifier: 0000-0002-5380-4948
authors.initials: R
authors.lastname: Geva
authors.affiliation: Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel ; Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Einat
authors.initials: E
authors.lastname: Shmueli
authors.affiliation: Department of Gastroenterology, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Aharon
authors.initials: A
authors.lastname: Hallack
authors.affiliation: The Integrated Cancer Prevention Center, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel ; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel ; Department of Gastroenterology, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Nadir
authors.initials: N
authors.lastname: Arber
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D000236:Adenoma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D000328:Adult; D000368:Aged; D051927:CD24 Antigen / Q000097:blood* / Q000235:genetics; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D051927:CD24 Antigen; C497889:CD24 protein, human