title: Bone Regeneration of Blood-derived Stem Cells within Dental Implants.
abstract: Peripheral blood (PB) is known as a source of mesenchymal stem cells (MSCs), as is bone marrow (BM), and is acquired easily. However, it is difficult to have enough MSCs, and their osteogenic capacity with dental implantations is scarce. Therefore, we characterized peripheral blood mesenchymal stem cells (PBMSCs) cultured on a bone marrow-derived mesenchymal stem cell (BMMSC) natural extracellular matrix (ECM) and demonstrated the osteogenic capability in an experimental chamber implant surgery model in rabbits. We isolated PBMSCs from rabbits by culturing on a natural ECM-coated plate during primary culture. We characterized the PBMSCs using a fluorescence-activated cell scanner, cell proliferation assay, and multiple differentiation assay and compared them with BMMSCs. We also analyzed the osteogenic potential of PBMSCs mixed with hydroxyapatite/tricalcium phosphate (HA/TCP) by transplanting them into immunocompromised mice. Then, the mixture was applied to the canals. After 3 and 6 wk, we analyzed new bone (NB) formation inside the chambers using histological and histomorphometric analyses. The PBMSCs had a similar rate of BrdU-positive cells to BMMSCs, positively expressing CD90 but negative for CD14. The PBMSCs also showed osteogenic, adipogenic, and chondrogenic ability in vitro and osteogenic ability in vivo. Histological and histomorphometric results illustrated that the PBMSC and BMMSC groups showed higher NB than the HA/TCP and defect groups in the upper and lower chambers at 6 wk and in the upper canal at 3 wk; however, there was no difference in NB among all groups in the lower canal at 3 wk. The PBMSCs have characteristics and bone regeneration ability similar to BMMSCs both in vitro and in vivo. ECM was effective for obtaining PBMSCs. Therefore, PBMSCs are a promising source for bone regeneration for clinical use.
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: R C
authors.initials: RC
authors.lastname: Zheng
authors.affiliation: Department of Dental Research Institute, Brain Korea 21, Seoul National University, Seoul, South Korea.
authors.forename: Y K
authors.initials: YK
authors.lastname: Park
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, South Korea ksy0617@snu.ac.kr jcho@snu.ac.kr.
authors.forename: S K
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Dental Regenerative Biotechnology, School of Dentistry, Seoul, South Korea.
authors.forename: J
authors.initials: J
authors.lastname: Cho
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Heo
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Koak
authors.affiliation: Department of Orthodontics & Dental Research Institute, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Prosthodontics, Seoul National University Gwanak Dental Hospital, Seoul, South Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, South Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001769:Blood; D001861:Bone Regeneration; D015921:Dental Implants; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015921:Dental Implants
keywords: bone marrow stromal cells; bone remodeling; dental implantation; extracellular matrix; mesenchymal stem cell; peripheral blood