title: Protective Effect of Micronized Purified Flavonoid Fraction on Ischemia/Reperfusion Injury of Rat Liver.
abstract: BACKGROUND
  Flavonoids have been subjected to considerable investigation because of its antioxidant and anti-inflammatory properties. However, there is no previously reported study about its effect on hepatic ischemia/reperfusion (I/R). We investigated the effects of micronized purified flavonoid fraction (MPFF) on hepatic I/R injury in rats.
  
  
  METHODS
  Thirty rats were recruited in the study as follows: group A, sham operation (n = 10); group B, I/R (n = 10); and group C, I/R+MPFF (n = 10). In group C, rats received (80 mg/kg/day) MPFF by gavage for 3 days before surgery, 30 minutes before ischemia and just before the reperfusion. Blood samples were taken, and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels were measured to assess liver functions. Liver tissues were taken for histological evaluation and to determine the total antioxidant capacity (TAC), catalase (CAT), total oxidant status (TOS), oxidative stress index (OSI), and myeloperoxidase (MPO).
  
  
  RESULTS
  The present data showed a decrease in AST, ALT, and LDH levels in the MPFF-treated rats when compared with I/R group rats (P < .001 for all). In the MPFF-treated rats, tissue levels of TOS, OSI, and MPO were significantly lower than those in the I/R group (P < .01, P < .001, and P < .05, respectively). Increases in TAC and CAT levels were statistically significant in the MPFF-treated rats compared with the I/R group (P = .01 for both). On the other hand, MPFF attenuated histological alterations that were induced by I/R.
  
  
  CONCLUSIONS
  The present study demonstrates that MPFF ameliorates I/R-induced liver damage, probably through antioxidant and anti-inflammatory properties.
authors.affiliation: Department of General Surgery, Gaziantep University School of Medicine, Gaziantep, Turkey. Electronic address: fahrettinyildiz@hotmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Yildiz
authors.affiliation: Department of General Surgery, Gaziantep University School of Medicine, Gaziantep, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Coban
authors.affiliation: Department of General Surgery, Harran University School of Medicine, Sanliurfa, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Terzi
authors.affiliation: Department of Biochemistry, Harran University School of Medicine, Sanliurfa, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Aksoy
authors.affiliation: Department of Pathology, Harran University School of Medicine, Sanliurfa, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Bitiren
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005419:Flavonoids / Q000627:therapeutic use*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008107:Liver Diseases / Q000209:etiology* / Q000517:prevention & control* / Q000601:surgery; D016031:Liver Transplantation / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000209:etiology* / Q000517:prevention & control*
chemical_list: D005419:Flavonoids; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D009195:Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase