title: Purification, Characterization, and Analysis of the Allergenic Properties of Myosin Light Chain in Procambarus clarkii.
abstract: Myosin light chain (MLC) plays a vital role in cell and muscle functions and has been identified as an allergen in shrimp. In this study, MLC with a molecular mass of 18 kDa was purified from crayfish (Procambarus clarkii) muscle. Its physicochemical characterization showed that the purified MLC is a glycoprotein with 4.3% carbohydrate, highly stable to heat, acid-alkali, and digestion, and weakly retains IgE-binding activity when its secondary structure was altered. Serological assays suggested that conformational epitopes predominate over linear epitopes in the purified MLC. Two isoforms of the MLC gene (MLC1 and MLC2) were cloned, and the purified MLC was identified as MLC1. Analysis of the secondary and tertiary structures of the MLCs indicated that MLC1 has four conformational epitopes and three linear epitopes, whereas MLC2 had a major conformational epitope and three linear epitopes. These results are significant for understanding hypersensitization of humans to crayfish.
authors.affiliation: †College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China.
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: †College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China.
authors.forename: Heng-Li
authors.initials: HL
authors.lastname: Chen
authors.affiliation: ‡Agriculture Research Service, Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
authors.forename: Soheila J
authors.initials: SJ
authors.lastname: Maleki
authors.affiliation: †College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China.
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Cao
authors.affiliation: †College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China.
authors.forename: Ling-Jing
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: †College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China.
authors.forename: Wen-Jin
authors.initials: WJ
authors.lastname: Su
authors.affiliation: †College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Liu
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003400:Astacoidea / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D018994:Myosin Light Chains / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D055550:Protein Stability; D012758:Shellfish / Q000032:analysis; D000067208:Shellfish Hypersensitivity / Q000097:blood / Q000276:immunology
chemical_list: D000485:Allergens; D060829:Arthropod Proteins; D000939:Epitopes; D018994:Myosin Light Chains; D020033:Protein Isoforms
keywords: Procambarus clarkii; allergenicity; epitopes; myosin light chain; physicochemical characterization; purification