title: [Effect of shikonin on proliferation of keratinocytes induced by interleukin-17 and expression of chemokines].
abstract: OBJECTIVE
  To observe the effect of shikonin on the proliferation of human keratinocytes induced by IL-17 and secretion of chemokines, in order to discuss the mechanism of Shikonin in the treatment of psoriasis.
  
  
  METHODS
  In vitro cultured HaCaT cells were stimulated by IL-17A (200 μg x L(-1)) and mixed with different concentrations (2, 1 mg x L(-1)) of shikonin for 24 hours. The cell proliferation was detected by CCK-8 assay. Cell secretion inflammatory factor interleukin-23 (IL-23) was detected by ELISA. The expressions of intracellular chemokines CXCL1, CXCL2, CCL20 and 6-defensin 4 (DEFB4) were detected by Real-time PCR.
  
  
  RESULTS
  Shikonin (2,1 mg x L(-1)) could distinctly inhibit HaCaT cell proliferation induced by IL-17A, with statistical difference (P < 0.01). Each shikonin group showed decreases in the secretion of IL-23 and inhibition in expressions of intracellular CXCL1, CXCL2, CCL20 and DEFB4.
  
  
  CONCLUSIONS
  Shikonin could inhibit HaCaT cells proliferation induced by IL-17 and secretion of relevant cytokines and recruit leukocytes by inhibiting chemokines, so as to show the effect in treating psoriasis.
authors.forename: Xin-ran
authors.initials: XR
authors.lastname: Xie
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.lastname: Zhang Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D018925:Chemokines / Q000235:genetics* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology*
chemical_list: D018925:Chemokines; D004365:Drugs, Chinese Herbal; D020381:Interleukin-17; D009285:Naphthoquinones; C016101:shikonin