title: A Comparative Proteomic Analysis of the Buds and the Young Expanding Leaves of the Tea Plant (Camellia sinensis L.).
abstract: Tea (Camellia sinensis L.) is a perennial woody plant that is widely cultivated to produce a popular non-alcoholic beverage; this beverage has received much attention due to its pleasant flavor and bioactive ingredients, particularly several important secondary metabolites. Due to the significant changes in the metabolite contents of the buds and the young expanding leaves of tea plants, high-performance liquid chromatography (HPLC) analysis and isobaric tags for relative and absolute quantitation (iTRAQ) analysis were performed. A total of 233 differentially expressed proteins were identified. Among these, 116 proteins were up-regulated and 117 proteins were down-regulated in the young expanding leaves compared with the buds. A large array of diverse functions was revealed, including roles in energy and carbohydrate metabolism, secondary metabolite metabolism, nucleic acid and protein metabolism, and photosynthesis- and defense-related processes. These results suggest that polyphenol biosynthesis- and photosynthesis-related proteins regulate the secondary metabolite content of tea plants. The energy and antioxidant metabolism-related proteins may promote tea leaf development. However, reverse transcription quantitative real-time PCR (RT-qPCR) showed that the protein expression levels were not well correlated with the gene expression levels. These findings improve our understanding of the molecular mechanism of the changes in the metabolite content of the buds and the young expanding leaves of tea plants.
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China. liqinvip@126.com.|National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. liqinvip@126.com.|Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. liqinvip@126.com.|Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, Hunan, China. liqinvip@126.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China. xixi_lj@126.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. shuoqianliuvip@126.com.
authors.forename: Shuoqian
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China. jian7513@gmail.com.|Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. jian7513@gmail.com.|Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, Hunan, China. jian7513@gmail.com.
authors.forename: Jianan
authors.initials: J
authors.lastname: Huang
authors.affiliation: National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. linhaiyanvip@126.com.|Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, Hunan, China. linhaiyanvip@126.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lin
authors.affiliation: National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. wkboo163@163.com.
authors.forename: Kunbo
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China. xiaomeichengvip@163.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China. larkin-liu@163.com.|National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. larkin-liu@163.com.|Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China. larkin-liu@163.com.|Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, Hunan, China. larkin-liu@163.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D028241:Camellia sinensis / Q000254:growth & development / Q000378:metabolism*; D002853:Chromatography, Liquid; D035264:Flowers / Q000254:growth & development / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D059467:Transcriptome / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020543:Proteome; D018749:RNA, Plant
keywords: Camellia sinensis L.; iTRAQ; proteome