title: Arsenic resistance and accumulation by two bacteria isolated from a natural arsenic contaminated site.
abstract: Forty-three indigenous arsenic resistant bacteria were isolated from arsenic rich soil of Rajnandgaon district in the state of Chhattisgarh, India by enrichment culture technique. Among the isolates, two of the bacteria (As-9 and As-14) exhibited high resistance to As(V) [MIC ≥ 700 mM] and As(III) [MIC ≥ 10 mM] and were selected for further studies. Both these bacteria grew well in the presence of arsenic [20 mM As(V) and 5 mM As(III)], but the isolate As-14 strictly required arsenic for its survival and growth and was characterized as a novel arsenic dependent bacterium. The isolates contributed to 99% removal of arsenic from the growth medium which was efficiently accumulated in the cell. Quantitative estimation of arsenic through Atomic Absorption Spectrophotometer revealed that there was >60% accumulation of both As(V) and As(III) by the two isolates. Scanning Electron Microscopic analysis showed a fourfold increase in bacterial cell volume when grown in the presence of arsenic and the results of Transmission Electron Microscopy and energy-dispersive X-ray spectroscopy proved that such an alteration was due to arsenic accumulation. Such arsenic resistant bacteria with efficient accumulating property could be effectively applied in the treatment of arsenic contaminated water.
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Pandey
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Bhatt
mesh_terms: D001151:Arsenic; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D005506:Food Contamination; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis; D013054:Spectrophotometry, Atomic
chemical_list: D012989:Soil Pollutants; D001151:Arsenic
keywords: Arsenate; Arsenite; Bioaccumulation; SEM; TEM