title: Alternative splicing of VEGFA, APP and NUMB genes in colorectal cancer.
abstract: OBJECTIVE
  To investigate alternative splicing in vascular endothelial growth factor A (VEGFA), amyloid beta precursor protein (APP), and Numb homolog (NUMB) in colorectal cancer (CRC).
  
  
  METHODS
  Real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and PCR-restriction fragment length polymorphism analyses were performed to detect the expression of VEGFA, APP, and NUMB mRNA in 20 CRC tissues and matched adjacent normal tissues, as well as their alternative splicing variants.
  
  
  RESULTS
  qRT-PCR analysis revealed that the expression of APP, NUMB, and VEGFA165b mRNA were significantly downregulated, while VEGFA mRNA was upregulated, in CRC tissues (all P < 0.05). PCR-restriction fragment length polymorphism analysis revealed that the expression of VEGFA165a/b in CRC tissues was significantly higher than in adjacent normal tissues (P < 0.05). Compared with adjacent normal tissues, the expression of NUMB-PRR(S) in CRC tissues was significantly decreased (P < 0.05), and the expression of NUMB-PRR(L) was increased (P < 0.05).
  
  
  CONCLUSIONS
  Alternative splicing of VEGFA, APP, and NUMB may regulate the development of CRC, and represent new targets for its diagnosis, prognosis, and treatment.
authors.affiliation: Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Zhao
authors.affiliation: Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Hua-Zhong
authors.initials: HZ
authors.lastname: Han
authors.affiliation: Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Chen-Zhang
authors.initials: CZ
authors.lastname: Shi
authors.affiliation: Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Qing-Chao
authors.initials: QC
authors.lastname: Zhu
authors.affiliation: Yi-Jun Zhao, Hua-Zhong Han, Chen-Zhang Shi, Qing-Chao Zhu, Jun Yang, Department of Surgery, Shanghai Jiao Tong University, Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D017398:Alternative Splicing; D054458:Amplified Fragment Length Polymorphism Analysis; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C545487:APP protein, human; D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C113026:NUMB protein, human; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Alternative splicing; Amyloid beta precursor protein; Colorectal cancer; Numb homolog; Vascular endothelial growth factor A