title: Evaluation of Amniotic Multipotential Tissue Matrix to Augment Healing of Demineralized Bone Matrix in an Animal Calvarial Model.
abstract: Amniotic multipotential tissue matrix (AmnioMTM) is a membrane material derived from placental tissues and rich in growth factors that have been reported to have potential in healing bone. This study hypothesized that demineralized bone matrix (DBM) supplemented with AmnioMTM would accelerate healing and bone formation as compared with DBM alone in a critical size (10 mm) rat calvarial bone defect model. Five DBM grafts and 5 DBM supplemented with AmnioMTM grafts were implanted in a 10-mm critical sized defect in 10 rats (1 implant per rat). After 4 weeks, animals were euthanized and defects evaluated by microCT and histology. There were no statistical differences in microCT data for mineral density, percent bone fill, or bone surface to volume ratios between groups, though the bone surface to volume ratio for the amnio-supplemented group suggested increased osteoid activity as compared with the DBM alone group. Histological data also indicated active osteoid activity and induced bone formation in the center of defects implanted with AmnioMTM supplemented graft as compared with DBM graft alone suggesting some potential osteoinductive potential. However, there was no significant difference at the mean percent of newly mineralized bone in the DBM group defect as compared with the AmnioMTM supplemented graft material. These data suggest that while bone formation was not increased at this early time point, the increased osteoid activity and the induction of new bone in the middle of the defect by the AmnioMTM indicates that further study is needed to assess its potential benefit to bone healing and regeneration.
authors.affiliation: *Department of Plastic Surgery †Department of Otolaryngology ‡Joint Biomedical Engineering Program, University of Memphis, University of Tennessee Health Science Center, Memphis, TN §Biomedical Engineering Department, University of Texas - San Antonio, San Antonio, TX.
authors.forename: Petros
authors.initials: P
authors.lastname: Konofaos
authors.forename: Dana
authors.initials: D
authors.lastname: Petersen
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Jennings
authors.forename: Richard A
authors.initials: RA
authors.lastname: Smith
authors.forename: Heather
authors.initials: H
authors.lastname: Doty
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Reves
authors.forename: Teja
authors.initials: T
authors.lastname: Guda
authors.forename: Mark R
authors.initials: MR
authors.lastname: Appleford
authors.forename: Joel D
authors.initials: JD
authors.lastname: Bumgardner
authors.forename: Robert D
authors.initials: RD
authors.lastname: Wallace
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001857:Bone Matrix / Q000637:transplantation*; D018786:Bone Substitutes; D019465:Craniofacial Abnormalities / Q000601:surgery*; D004195:Disease Models, Animal; D051381:Rats; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures / Q000379:methods*; D014945:Wound Healing; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes