title: [Anti-complementary phenolic acids from Lonicera japonica].
abstract: OBJECTIVE
  To study the anti-complementary phenolic acids from Lonicera japonica.
  
  
  METHODS
  The anti-complementary activity-directed isolation was carried out with the hemolysis test as guide. All isolation was evaluated for their in vitro anti-complementary activities. The structures were identified by various spectroscopic data including ESI-MS, 1H-NMR, 13C-NMR data.
  
  
  RESULTS
  Fourteen compounds were isolated from the EtOAc fraction of L. japonica extracts, including 8 phenolic acids: 5-O-caffeoylquinic acid (1), chlorogenic (2), 4-O-caffeoylquinic acid (3), 3,5-di-O-caffeoylquinic acid (4), 4,5-di-O-caffeoylquinic acid (5), 3,4-di-O-caffeoylquinic acid (6), caffeic acid (7) and methyl caffeate acid (8); 3 iridoids: secologanoside (9), sweroside (10) and secoxyloganin (11); and 3 flavonoids: luteolin (12), quercetin (13) and kaempferol (14). Compounds 1-9 and 11-14 showed anti-complementary activity in different extents and 3,5-di-O-caffeoylquinic acid (4) exhibited the most significant activity against the classical pathway.
  
  
  CONCLUSIONS
  Compound 14 is obtained from this plant for the first time, phenolic acids are the main anti-complementary constituents of L. japonica and 3,5-di-O-caffeoylquinic acid(4) is a potential complement inhibitor with strong activity, which worthy to be studied further in the future.
authors.forename: Fu-yong
authors.initials: FY
authors.lastname: Ni
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Song
authors.forename: Xue-jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yi-wu
authors.initials: YW
authors.lastname: Zhao
authors.forename: Wen-zhe
authors.initials: WZ
authors.lastname: Huang
authors.forename: Zhen-zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D051056:Complement Inactivating Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031239:Lonicera / Q000737:chemistry*
chemical_list: D051056:Complement Inactivating Agents; D062385:Hydroxybenzoates