title: Valuable lessons-learned in transcriptomics experimentation.
abstract: We have collected several valuable lessons that will help improve transcriptomics experimentation. These lessons relate to experiment design, execution, and analysis. The cautions, but also the pointers, may help biologists avoid common pitfalls in transcriptomics experimentation and achieve better results with their transcriptome studies.
authors.affiliation: a RNA Biology & Applied Bioinformatics research group; Swammerdam Institute for Life Sciences; Faculty of Science; University of Amsterdam ; Amsterdam , the Netherlands.
authors.forename: Oskar
authors.initials: O
authors.lastname: Bruning
authors.forename: Han
authors.initials: H
authors.lastname: Rauwerda
authors.forename: Rob J
authors.initials: RJ
authors.lastname: Dekker
authors.forename: Wim C
authors.initials: WC
authors.lastname: de Leeuw
authors.forename: Paul F K
authors.initials: PF
authors.lastname: Wackers
authors.forename: Wim A
authors.initials: WA
authors.lastname: Ensink
authors.forename: Martijs J
authors.initials: MJ
authors.lastname: Jonker
authors.forename: Timo M
authors.initials: TM
authors.lastname: Breit
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D012107:Research Design; D017423:Sequence Analysis, RNA; D012984:Software; D059467:Transcriptome
keywords: Design for experimentation; Transcriptomics; experimental analysis; experimental execution; lessons-learned