title: Oligomeric assembly is required for chaperone activity of the filamentous γ-prefoldin.
abstract: Prefoldins (PFDs) are molecular chaperones with a distinctive jellyfish-shape that have a general role in de novo protein folding in Archaea and in the biogenesis of cytoskeleton proteins in eukaryotes. In general, PFDs are hetero-hexameric protein assemblies consisting of two α and four β subunits. However, a PFD variant called gamma-prefoldin (γPFD), isolated from the hyperthermophilic archaeon Methanocaldococcus jannaschii, exhibits a unique filamentous structure that is composed of hundreds of monomeric subunits. In this study, we investigated the relationship between the morphology of the γPFD filament and its ability to prevent protein aggregation. A chaperone assay demonstrated that γPFD must be in a filamentous assembly for functional activity and the distal regions of the coiled-coils are required for binding of non-native proteins. Molecular dynamic simulations were used to model the interactions between in silico thermally denatured protein substrates and the coiled-coils of a γPFD filament. During molecular dynamic simulations at 300 and 353 K, each coiled-coil was highly flexible, enabling it to widen the central cavity of the filament to potentially capture various non-native proteins. Docking molecular dynamic simulations of γPFD filaments with unfolded citrate synthase or insulin showed a size-dependence between the substrate and the number of interacting coiled-coils. To confirm this observation, we generated filaments containing specific numbers of subunits, and showed that between six and eight γPFD subunits are required for chaperone activity to prevent citrate synthase from thermal aggregation. These results provide insights into structure-function relationships of oligomeric chaperones and illuminate the potential role of γPFD in its native environment.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Glover
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
authors.forename: Douglas S
authors.initials: DS
authors.lastname: Clark
mesh_terms: D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism*; D063747:Methanocaldococcus / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D056004:Molecular Dynamics Simulation
chemical_list: D001704:Biopolymers; D018832:Molecular Chaperones; C112806:prefoldin
keywords: filamentous; holdase chaperone; molecular dynamics; substrate binding; thermophilic protein