title: Biochemical and hormonal analysis of follicular fluid and serum of female dromedary camels (Camelus dromedarius) with different sized ovarian follicles.
abstract: The current study aimed to compare some biochemical and hormonal constituents in follicular fluids and serum of female dromedary camels with different sized ovarian follicles. Therefore, follicular fluids from follicles sized 1.1-1.5cm (n=10), 1.6-2.1cm (n=10) and 2.2-2.5cm (n=10) and sera were harvested from 20 female camels. The concentrations of ascorbic acid, glucose, cholesterol and activities of acid phosphatase (ACP) and alkaline phosphatase (ALP) were not changed significantly neither in follicular fluids of all follicle sizes nor in sera of female camels with different sized follicles. The concentrations of estradiol-17β (E2) in the follicular fluid of follicles sized 2.2-2.5cm were significantly lower (P<0.01) than its corresponding value in follicular fluid of other follicle sizes. The concentrations of progesterone (P4), tri-iodothyronine (T3), thyroxin (T4), cortisol and insulin-like growth factor-1 (IGF-1) remained comparable in follicular fluids of all examined different sized follicles. The concentrations of E2, P4, T3, T4, cortisol and IGF-1 were similar in the serum of camels with different sized follicles. Interestingly, mean concentrations of P4 and IGF-1 in follicular fluids were higher than their corresponding values in sera of camels with different sized follicles and the mean concentrations of glucose, cholesterol, ALP and cortisol in sera were higher than their corresponding values in follicular fluids of the examined camels. With the exception of E2, there were no significant differences in biochemical and hormonal constituents between follicular fluids from different sized follicles.
authors.affiliation: Department of Physiology, Biochemistry and Pharmacology (Biochemistry), College of Veterinary Medicine and Animal Resources, King Faisal University, Saudi Arabia; Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Egypt. Electronic address: sabryelbahr@hotmail.com.
authors.forename: S M
authors.initials: SM
authors.lastname: El-Bahr
authors.affiliation: Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Saudi Arabia.
authors.forename: I M
authors.initials: IM
authors.lastname: Ghoneim
authors.affiliation: Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Saudi Arabia.
authors.forename: M M
authors.initials: MM
authors.lastname: Waheed
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D002162:Camelus / Q000033:anatomy & histology / Q000097:blood / Q000502:physiology*; D002784:Cholesterol / Q000032:analysis; D004958:Estradiol / Q000032:analysis* / Q000097:blood; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006854:Hydrocortisone / Q000032:analysis / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D006080:Ovarian Follicle / Q000033:anatomy & histology* / Q000502:physiology; D011374:Progesterone / Q000032:analysis / Q000097:blood; D013974:Thyroxine / Q000032:analysis / Q000097:blood; D014284:Triiodothyronine / Q000032:analysis / Q000097:blood
chemical_list: D014284:Triiodothyronine; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D002784:Cholesterol; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005947:Glucose; D001205:Ascorbic Acid; D013974:Thyroxine; D006854:Hydrocortisone
keywords: Biochemistry; Camels; Different sized follicles; Follicular fluid; Hormones; Serum