title: Distribution of petroleum degrading genes and factor analysis of petroleum contaminated soil from the Dagang Oilfield, China.
abstract: Genes that encode for enzymes that can degrade petroleum hydrocarbons (PHs) are critical for the ability of microorganisms to bioremediate soils contaminated with PHs. Distributions of two petroleum-degrading genes AlkB and Nah in soils collected from three zones of the Dagang Oilfield, Tianjin, China were investigated. Numbers of copies of AlkB ranged between 9.1 × 10(5) and 1.9 × 10(7) copies/g dry mass (dm) soil, and were positively correlated with total concentrations of PHs (TPH) (R(2) = 0.573, p = 0.032) and alkanes (C33 ~ C40) (R(2) = 0.914, p < 0.01). The Nah gene was distributed relatively evenly among sampling zones, ranging between 1.9 × 10(7) and 1.1 × 10(8) copies/g dm soil, and was negatively correlated with concentrations of total aromatic hydrocarbons (TAH) (R(2) = -0.567, p = 0.035) and ∑16 PAHs (R(2) = -0.599, p = 0.023). Results of a factor analysis showed that individual samples of soils were not ordinated as a function of the zones.
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Center of Pollution Diagnosis and Environmental Restoration, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Center of Pollution Diagnosis and Environmental Restoration, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Bai
authors.affiliation: 1] School of Environment and sustainability, University of Saskatchewan, Saskatoon, Saskatchewan, Canada [2] Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Hecker
authors.affiliation: 1] Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada [2] Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada [3] School of Biological Sciences, University of Hong Kong, Hong Kong, SAR, China [4] State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China [5] Department of Biology, Hong Kong Baptist University, Hong Kong, SAR, China.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
mesh_terms: D002681:China; D005163:Factor Analysis, Statistical; D054892:Metagenome; D064307:Microbiota; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*
chemical_list: D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants