title: Serum MicroRNA Profiles Serve as Novel Biomarkers for the Diagnosis of Alzheimer's Disease.
abstract: Alzheimer's disease (AD) is the most common type of dementia, and promptly diagnosis of AD is crucial for delaying the development of disease and improving patient quality of life. However, AD detection, particularly in the early stages, remains a substantial challenge due to the lack of specific biomarkers. The present study was undertaken to identify and validate the potential of circulating miRNAs as novel biomarkers for AD. Solexa sequencing was employed to screen the expression profile of serum miRNAs in AD and controls. RT-qPCR was used to confirm the altered miRNAs at the individual level. Moreover, candidate miRNAs were examined in the serum samples of patients with mild cognitive impairment (MCI) and vascular dementia (VD). The results showed that four miRNAs (miR-31, miR-93, miR-143, and miR-146a) were markedly decreased in AD patients' serum compared with controls. Receiver operating characteristic curve analysis demonstrated that this panel of four miRNAs could be used as potential biomarker for AD. Furthermore, miR-93, and miR-146a were significantly elevated in MCI compared with controls, and the panel of miR-31, miR-93 and miR-146a can be used to discriminate AD from VD. We established a panel of four serum miRNAs as a novel noninvasive biomarker for AD diagnosis.
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China ; Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China ; Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
authors.forename: Jialu
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Donghai
authors.identifier: 0000-0003-1948-9291
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Geriatric Psychiatry, Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China ; Alzheimer's Disease and Related Disorders Center, Shanghai Jiaotong University, Shanghai 200000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Neurology, Jiangbin Hospital, Guangxi Zhuang Autonomous Region, Nanning 530021, China.
authors.forename: Caiyou
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Neurology, Nanjing Brain Hospital, Nanjing 210029, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China ; Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Chunni
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.affiliation: Department of Geriatric Psychiatry, Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China ; Alzheimer's Disease and Related Disorders Center, Shanghai Jiaotong University, Shanghai 200000, China.
authors.forename: Shifu
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China ; Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D015415:Biomarkers / Q000097:blood*; D060825:Cognitive Dysfunction / Q000097:blood / Q000235:genetics*; D015140:Dementia, Vascular / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D012372:ROC Curve; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D015415:Biomarkers; C542359:MIRN143 microRNA, human; C542542:MIRN146 microRNA, human; C534612:MIRN31 microRNA, human; C543350:MIRN93 microRNA, human; D035683:MicroRNAs