title: Influence of surface microstructure and chemistry on osteoinduction and osteoclastogenesis by biphasic calcium phosphate discs.
abstract: It has been reported that surface microstructural dimensions can influence the osteoinductivity of calcium phosphates (CaPs), and osteoclasts may play a role in this process. We hypothesised that surface structural dimensions of ≤ 1 μm trigger osteoinduction and osteoclast formation irrespective of macrostructure (e.g., concavities, interconnected macropores, interparticle space) or surface chemistry. To test this, planar discs made of biphasic calcium phosphate (BCP: 80% hydroxyapatite, 20% tricalcium phosphate) were prepared with different surface structural dimensions - either ~ 1 μm (BCP1150) or ~ 2-4 μm (BCP1300) - and no macropores or concavities. A third material was made by sputter coating BCP1150 with titanium (BCP1150Ti), thereby changing its surface chemistry but preserving its surface structure and chemical reactivity. After intramuscular implantation in 5 dogs for 12 weeks, BCP1150 formed ectopic bone in 4 out of 5 samples, BCP1150Ti formed ectopic bone in 3 out of 5 samples, and BCP1300 formed no ectopic bone in any of the 5 samples. In vivo, large multinucleated osteoclast-like cells densely colonised BCP1150, smaller osteoclast-like cells formed on BCP1150Ti, and osteoclast-like cells scarcely formed on BCP1300. In vitro, RAW264.7 cells cultured on the surface of BCP1150 and BCP1150Ti in the presence of osteoclast differentiation factor RANKL (receptor activator for NF-κB ligand) proliferated then differentiated into multinucleated osteoclast-like cells with positive tartrate resistant acid phosphatase (TRAP) activity. However, cell proliferation, fusion, and TRAP activity were all significantly inhibited on BCP1300. These results indicate that of the material parameters tested - namely, surface microstructure, macrostructure, and surface chemistry - microstructural dimensions are critical in promoting osteoclastogenesis and triggering ectopic bone formation.
authors.affiliation: Xpand Biotechnology, Professor Bronkhorstlaan 10 Bldg 48, 3723 MB Bilthoven, The Netherlands.noel.davison@xpand-biotech.com.
authors.forename: N L
authors.initials: NL
authors.lastname: Davison
authors.forename: J
authors.initials: J
authors.lastname: Su
authors.forename: H
authors.initials: H
authors.lastname: Yuan
authors.forename: J J J P
authors.initials: JJ
authors.lastname: van den Beucken
authors.forename: J D
authors.initials: JD
authors.lastname: de Bruijn
authors.forename: F
authors.initials: F
authors.lastname: Barrère-de Groot
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004285:Dogs; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D019736:Prostheses and Implants; D013499:Surface Properties; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D014025:Titanium / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; D007527:Isoenzymes; C074950:hydroxyapatite-beta tricalcium phosphate; D014025:Titanium; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; C018392:tricalcium phosphate