title: Pteridic acid hydrate and pteridic acid C produced by StreStreptomyces pseudoverticillus YN17707 induce cell cycle arrest.
abstract: The present study aimed at identifying cell cycle inhibitors from the fermentation broth of Streptomyces pseudoverticillus YN17707. Activity-guided isolation was performed on tsFT210 cells. Compounds were isolated through various chromatographic methods and elucidated by spectroscopic analyses. Flow cytometry was used to evaluate the cell cycle inhibitory activities of the fractions and compounds. Two compounds were obtained and identified as pteridic acid hydrate (1) and pteridic acid C (2), which arrested the tsFT210 cells at the G0/G1 phase with the MIC values being 32.8 and 68.9 μmol·L(-1), respectively. These results provide a basis for future development of Compounds 1 and 2 as novel cell cycle inhibitors for cancer therapy.
authors.affiliation: Beijing Institute of Biomedicine, Beijing 100091, China. Electronic address: h3b3@sohu.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Beijing Institute of Biomedicine, Beijing 100091, China.
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Li
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Cui
mesh_terms: D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D002460:Cell Line; D006538:Heptanoic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D006538:Heptanoic Acids; D013141:Spiro Compounds; C467219:pteridic acid A; C000605373:pteridic acid C; C000605372:pteridic acid hydrate
keywords: Cell cycle inhibitor; Flow cytometry; Pteridic acid; Spirocyclic polyketide; Streptomyces pseudoverticillus