title: Adipose-Derived Stromal Vascular Fraction Differentially Expands Breast Progenitors in Tissue Adjacent to Tumors Compared to Healthy Breast Tissue.
abstract: BACKGROUND
  Autologous fat grafts supplemented with adipose-derived stromal vascular fraction are used in reconstructive and cosmetic breast procedures. Stromal vascular fraction contains adipose-derived stem cells that are thought to encourage wound healing, tissue regeneration, and graft retention. Although use of stromal vascular fraction has provided exciting perspectives for aesthetic procedures, no studies have yet been conducted to determine whether its cells contribute to breast tissue regeneration. The authors examined the effect of these cells on the expansion of human breast epithelial progenitors.
  
  
  METHODS
  From patients undergoing reconstructive breast surgery following mastectomies, abdominal fat, matching tissue adjacent to breast tumors, and the contralateral non-tumor-containing breast tissue were obtained. Ex vivo co-cultures using breast epithelial cells and the stromal vascular fraction cells were used to study the expansion potential of breast progenitors. Breast reduction samples were collected as a source of healthy breast cells.
  
  
  RESULTS
  The authors observed that progenitors present in healthy breast tissue or contralateral non-tumor-containing breast tissue showed significant and robust expansion in the presence of stromal vascular fraction (5.2- and 4.8-fold, respectively). Whereas the healthy progenitors expanded up to 3-fold without the stromal vascular fraction cells, the expansion of tissue adjacent to breast tumor progenitors required the presence of stromal vascular fraction cells, leading to a 7-fold expansion, which was significantly higher than the expansion of healthy progenitors with stromal vascular fraction.
  
  
  CONCLUSIONS
  The use of stromal vascular fraction might be more beneficial to reconstructive operations following mastectomies compared with cosmetic corrections of the healthy breast. Future studies are required to examine the potential risk factors associated with its use.
  
  
  METHODS
  Therapeutic, V.
authors.affiliation: Winnipeg, Manitoba, Canada From the Department of Immunology, the Department of Surgery, Section of Plastic Surgery, and the Department of Pathology, Faculty of Health Sciences, University of Manitoba.
authors.forename: Sumanta
authors.initials: S
authors.lastname: Chatterjee
authors.forename: Mike
authors.initials: M
authors.lastname: Laliberte
authors.forename: Sarah
authors.initials: S
authors.lastname: Blelloch
authors.forename: Imran
authors.initials: I
authors.lastname: Ratanshi
authors.forename: Janice
authors.initials: J
authors.lastname: Safneck
authors.forename: Ed
authors.initials: E
authors.lastname: Buchel
authors.forename: Afshin
authors.initials: A
authors.lastname: Raouf
mesh_terms: D000328:Adult; D001940:Breast / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D001943:Breast Neoplasms / Q000601:surgery*; D018270:Carcinoma, Ductal, Breast / Q000601:surgery*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D016462:Mammaplasty / Q000379:methods*; D008408:Mastectomy; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D012038:Regeneration; D013234:Stem Cells / Q000502:physiology; D050507:Subcutaneous Fat, Abdominal / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D016896:Treatment Outcome