title: [Lycopene attenuates angiotensin II induced oxidative stress in H9c2 cells].
abstract: OBJECTIVE
  To investigate the effect of Lycopene (Lyc) on Ang II induced oxidative stress in H9c2 cell line derived from rat cardiac tissue,and to explore related mechanisms.
  
  
  METHODS
  H9c2 cells were divided into 6 groups: control group, Ang II group (1 µmol/L), Ang II (1 µmol/L) + low dose Lyc (3.125 nmol/L) group, Ang II (1 µmol/L) + moderate dose Lyc (6.25 nmol/L) group and Ang II (1 µmol/L) + high dose Lyc (12.5 nmol/L) group and Lyc group (12.5 nnmol/L). Cell growth was determined by CCK8 assay, ROS generation was detected using a Microplate reader and Fluorescence microscopy, the expression of NOX2 was determined by Western blot, mRNA expression of p47(phox), SOD1 and SOD2 were determined by Real Time-PCR, MDA was detected by ELISA kit.
  
  
  RESULTS
  Compared to control group,cell survival was significantly reduced and ROS generation was significantly increased post Ang II stimulation,cotreatment with Lyc significantly improved cell survival and reduced ROS generation in a dose-dependent manner (all P < 0.01). mRNA expression of SOD1 and SOD2 was significantly downregulated while MDA concentration was significantly increased in Ang II treated cells, which could be significantly reversed by cotreatment with Lyc in a dose dependent manner (all P < 0.01). Protein expression of NOX2 and mRNA expression of p47(phox) were significantly upregulated post Ang II and which could be significantly downregulated by cotreatment with Lyc in a dose-dependent manner (all P < 0.01).
  
  
  CONCLUSIONS
  Lyc could attenuate Ang II induced oxidative stress and this effect is linked with its capacity of reducing ROS generation and enhancing cellular ROS scavenging ability in H9c2 cells.
authors.affiliation: Department of Cardiology, Ren Min Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Ren Min Hospital of Wuhan University, Wuhan 430060, China; Email: qztang@whu.edu.cn.
authors.forename: Qizhu
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Ren Min Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Zhefu
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Cardiology, Ren Min Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.affiliation: Department of Cardiology, Ren Min Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Li
mesh_terms: D000804:Angiotensin II; D000818:Animals; D002338:Carotenoids / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D015536:Down-Regulation; D006321:Heart; D000077276:Lycopene; D018384:Oxidative Stress; D012333:RNA, Messenger; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000804:Angiotensin II; D002338:Carotenoids; D000077276:Lycopene