title: Regulatory B cell-specific interleukin-10 is dispensable for atherosclerosis development in mice.
abstract: OBJECTIVE
  To determine the role of regulatory B cell-derived interleukin (IL)-10 in atherosclerosis.
  
  
  RESULTS
  We created chimeric Ldlr(-/-) mice with a B cell-specific deficiency in IL-10, and confirmed that purified B cells stimulated with lipopolysaccharide failed to produce IL-10 compared with control Ldlr(-/-) chimeras. Mice lacking B-cell IL-10 demonstrated enhanced splenic B-cell numbers but no major differences in B-cell subsets, T cell or monocyte distribution, and unchanged body weights or serum cholesterol levels compared with control mice. After 8 weeks on high-fat diet, there were no differences in aortic root or aortic arch atherosclerosis. In addition to plaque size, plaque composition (macrophages, T cells, smooth muscle cells, and collagen) was similar between groups.
  
  
  CONCLUSIONS
  In contrast to its prominent regulatory role in many immune-mediated diseases and its proposed modulatory role in atherosclerosis, B cell-derived IL-10 does not alter atherosclerosis in mice.
authors.affiliation: From the Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, United Kingdom (A.P.S, M.S, L.L.B., A.J.F., L.M.M., Z.M.); and Institut National de la Sante et de la Recherche Medicale, Paris, France (Z.M.).
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Sage
authors.affiliation: From the Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, United Kingdom (A.P.S, M.S, L.L.B., A.J.F., L.M.M., Z.M.); and Institut National de la Sante et de la Recherche Medicale, Paris, France (Z.M.).
authors.forename: Meritxell
authors.initials: M
authors.lastname: Nus
authors.affiliation: From the Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, United Kingdom (A.P.S, M.S, L.L.B., A.J.F., L.M.M., Z.M.); and Institut National de la Sante et de la Recherche Medicale, Paris, France (Z.M.).
authors.forename: Lauren L
authors.initials: LL
authors.lastname: Baker
authors.affiliation: From the Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, United Kingdom (A.P.S, M.S, L.L.B., A.J.F., L.M.M., Z.M.); and Institut National de la Sante et de la Recherche Medicale, Paris, France (Z.M.).
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Finigan
authors.affiliation: From the Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, United Kingdom (A.P.S, M.S, L.L.B., A.J.F., L.M.M., Z.M.); and Institut National de la Sante et de la Recherche Medicale, Paris, France (Z.M.).
authors.forename: Leanne M
authors.initials: LM
authors.lastname: Masters
authors.affiliation: From the Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, United Kingdom (A.P.S, M.S, L.L.B., A.J.F., L.M.M., Z.M.); and Institut National de la Sante et de la Recherche Medicale, Paris, France (Z.M.). zm255@medschl.cam.ac.uk.
authors.forename: Ziad
authors.initials: Z
authors.lastname: Mallat
mesh_terms: D000818:Animals; D001011:Aorta / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001018:Aortic Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D060151:B-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002478:Cells, Cultured; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic; D011973:Receptors, LDL / Q000172:deficiency / Q000235:genetics; D013997:Time Factors
chemical_list: D015415:Biomarkers; C556062:IL10 protein, mouse; D011973:Receptors, LDL; D016753:Interleukin-10
keywords: atherosclerosis; interleukins; lymphocytes