title: Blood Glucose-lowering Effect of T. procumbens L.: A Pilot Clinical Study in Individuals with Type 2 Diabetes.
abstract: Traditional knowledge, in vitro studies, and studies using animal models suggest that Tridax procumbens L. exhibits blood glucose-lowering properties and antiinflammatory effects. In this study, we evaluated the blood glucose-lowering effect of T. procumbens supplementation in individuals with type 2 diabetes. An extract (asava) of T. procumbens L. was prepared following Ayurveda guidelines. Chemical and microbial analyses indicated presence of phenolics, flavonoids, and carotenoids, and absence of microbial contamination, aflatoxins, heavy metals, and pesticide residues. A chemical fingerprint of T. procumbens L. asava, developed using Ultra high pressure liquid chromatography/electron spray ionization-mass spectrometry (UPLC/ESI-MS) in negative mode, suggest the presence of several compounds including polyphenols. T. procumbens asava demonstrated strong total antioxidant capacity, Fe3+reducing potential, Fe2+chelation, H2O2scavenging activity, and inhibition of lipid peroxidation. We recruited 20 type 2 diabetic individuals from Kolhapur, India. Participants received 15 mL of T. procumbens asava, twice daily, for 4 weeks, while continuing their prescribed antidiabetic medications. Fasting blood glucose decreased by 11% in men (p < 0.01) and 20% in women (p < 0.05), and post-prandial blood glucose concentrations were lowered by 26% in men (p < 0.001) and 29% in women (p < 0.001) following 4 weeks of asava supplementation. No adverse events or side effects were reported. This is the first clinical study demonstrating a significant blood glucose-lowering effect of T. procumbens asava in type 2 diabetes. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Nutrition and Dietetics, Boshell Diabetes and Metabolic Diseases Research Program, Auburn University, Auburn, AL, 36830, USA.|Bhagawati Industrial and Organic Products, Kolhapur, Maharashtra, India.
authors.forename: Gauri S
authors.initials: GS
authors.lastname: Desai
authors.affiliation: Bhagawati Industrial and Organic Products, Kolhapur, Maharashtra, India.
authors.forename: Shirish V
authors.initials: SV
authors.lastname: Desai
authors.affiliation: GTL Foundation, Sindhudurg, Maharashtra, India.
authors.forename: Rajendra S
authors.initials: RS
authors.lastname: Gavaskar
authors.affiliation: Department of Chemical Engineering, Tuskegee University, Tuskegee, AL, 36088, USA.
authors.forename: Vanisree
authors.initials: V
authors.lastname: Mulabagal
authors.affiliation: Department of Chemistry and Biochemistry, Auburn University, Auburn, AL, 36849, USA.
authors.forename: Yonnie
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Nutrition and Dietetics, Boshell Diabetes and Metabolic Diseases Research Program, Auburn University, Auburn, AL, 36830, USA.
authors.forename: Suresh T
authors.initials: ST
authors.lastname: Mathews
keywords: Tridax procumbens; antioxidants; blood glucose control; diabetes