title: Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth.
abstract: Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. Mesenchymal stem cells (MSCs) have also been shown to be involved in this process of repair. However, the mechanisms through which MSCs are recruited to the DP have not yet been elucidated. Therefore, the aim of the present in vitro study was to investigate whether stromal cell-derived factor 1α (SDF1)-C-X-C chemokine receptor type 4 (CXCR4) signaling is involved in tissue repair in the DP of deciduous teeth. A single-cell clone from DPCs (SDP11) and UE7T-13 cells were used as pulp cells and MSCs, respectively. The MG-63 and HuO9 cells, two osteosarcoma cell lines, were used as positive control cells. Reverse transcription polymerase chain reaction (RT-PCR) revealed that all cell lines (SDP11, UE7T-13 MG-63 and HuO9) were positive for both SDF1 and CXCR4 mRNA expression. Moreover, immunocytochemical analysis indicated that SDF1 and CXCR4 proteins were expressed in the SDP11 and UE7T-13 cells. SDF1 was also detected in the cell lysates (CLs) and conditioned medium (CM) collected from the SDP11 and UE7T-13 cells, and AMD3100, a specific antagonist of CXCR4, inhibited the migration of the UE7T-13 cells; this migration was induced by treatment with CM, which was collected from the SDP11 cells. In addition, real-time PCR showed that the expression of SDF1 in the SDP11 cells was inhibited by treatment with 20 ng/ml fibroblast growth factor (FGF)-2, and exposure to AZD4547, an inhibitor of the FGF receptor, blocked this inhibition. Collectively, these data suggest that SDF1 produced by DP plays an important role in homeostasis, repair and regeneration via the recruitment of MSCs.
authors.affiliation: Department of Pediatric Dentistry, Tokushima University Hospital, Tokushima 770‑8504, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Akazawa
authors.affiliation: Department of Pediatric Dentistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770‑8504, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Hasegawa
authors.affiliation: Department of Molecular Cell Pharmacology, Division of Oral Pathological Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Hokkaido 060-8586, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yoshimura
authors.affiliation: Department of Oral Biochemistry, School of Dentistry, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Chosa
authors.affiliation: Department of Special Needs Dentistry, Division of Dentistry for Persons with Disabilities, Showa University School of Dentistry, Tokyo 145-8515, Japan.
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Asakawa
authors.affiliation: Department of Pediatric Dentistry, Tokushima University Hospital, Tokushima 770‑8504, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Ueda
authors.affiliation: Department of Pediatric Dentistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770‑8504, Japan.
authors.forename: Asuna
authors.initials: A
authors.lastname: Sugimoto
authors.affiliation: Department of Pediatric Dentistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770‑8504, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Kitamura
authors.affiliation: Department of Pediatric Dentistry, Tokushima University Hospital, Tokushima 770‑8504, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakagawa
authors.affiliation: Department of Oral Biochemistry, School of Dentistry, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ishisaki
authors.affiliation: Department of Pediatric Dentistry, Tokushima University Hospital, Tokushima 770‑8504, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Iwamoto
mesh_terms: D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002648:Child; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014094:Tooth, Deciduous / Q000166:cytology* / Q000378:metabolism
chemical_list: D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D016222:Fibroblast Growth Factor 2