title: Synergistic effects of amine and protein modified epoxy-support on immobilized lipase activity.
abstract: We have developed an improved and effective method to immobilize Yarrowia lipolytica lipase Lip2 (YLIP2) on an epoxy poly-(glycidylmethacrylate-triallyisocyanurate-ethyleneglycoldimethacrylate) (PGMA-TAIC-EGDMA) support structure with or without amine or/and protein modifications. Our results show that there is an increase in the activity of the immobilized lipase on n-butylamine (BA) modified support (420U/g support) and the biocompatible gelatin modified support (600U/g support) when compared to the support without modification (240U/g support). To further study the influences of BA and gelatin modification on the activity of the immobilized lipase, gelatin and BA were concurrently used to decorate the support structure. Lipase immobilized on 2% BA/gelatin (1:1) modified support obtained the highest activity (1180U/g support), which was five-fold higher than that on a native support structure. These results suggest that the activity of a support-immobilized lipase depends on the support surface properties and a moderate support surface micro-environment was crucial for elevated activity. Collectively, these data show that a combined gelatin and BA modification regulates the support surface more suitable for immobilizing YLIP2.
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Cui
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Tao
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Ge
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yueju
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: chenbiqiang@gmail.com.
authors.forename: Biqiang
authors.initials: B
authors.lastname: Chen
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: twtan@mail.buct.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D000588:Amines / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D011506:Proteins; D008049:Lipase
keywords: Balance; Biocompatibility; Hydrophobic; Immobilization; Ionic; Lipase