title: Interleukin-10 promotor -592A/C polymorphism is associated with slow coronary flow in Han Chinese.
abstract: An accumulating body of evidence suggests that slow coronary flow (SCF) phenomenon seems to be an early-form of atherosclerosis and low-grade inflammation plays a major role in the atherosclerotic vascular processes. Interleukin (IL)-10 is a multifunctional cytokine involved in both innate and adaptive immune response. The aim of the present study is to investigate the association of IL-10 gene -592A/C polymorphism with SCF in Han Chinese. 250 patients who underwent coronary angiography and had angiographically normal coronary arteries of varying coronary flow rates without any atherosclerotic lesion were enrolled in this study. Patients who had thrombolysis in myocardial infarction frame counts (TFC) above the normal cutoffs were considered to have SCF and those within normal limits were considered to have normal coronary flow (NCF). The PCR-based restriction fragment length polymorphism (PCR-RFLP) technique was used to assess the genotypes frequencies. The distribution of the IL-10 -592A/C genotypes (AA, AC, and CC) was 46.34%, 41.46%, and 12.20% in the NCF group, and 66.51%, 28.71%, and 4.78% in SCF subjects, respectively (P = 0.0280). The frequency of the A allele in the SCF group was significantly higher than that in the NCF group (80.86% vs. 67.07%, P = 0.0054). Compared with the CC genotype, the AA genotype had increased risk of SCF in both unadjusted and adjusted analyses. In SCF patients, the average serum IL-10 levels in AA genotype were statistically lower than in AC + CC genotype (P = 0.0000). These findings suggest that IL-10 -592A/C polymorphism is associated with SCF and the A allele has increased risk for SCF in Han Chinese.
authors.affiliation: Department of Cardiology, Xinghua People's Hospital Xinghua 225700, P. R. China.
authors.forename: Gui-Liang
authors.initials: GL
authors.lastname: Shi
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, P. R. China.
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Cai
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, P. R. China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Su
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, P. R. China.
authors.forename: Chu
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Xinghua People's Hospital Xinghua 225700, P. R. China.
authors.forename: Xin-Tao
authors.initials: XT
authors.lastname: Deng
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University Nantong 226001, P. R. China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Pan
authors.affiliation: Department of Cardiology, Xinghua People's Hospital Xinghua 225700, P. R. China.
authors.forename: Meng-Kan
authors.initials: MK
authors.lastname: Fan
authors.affiliation: Department of Cardiology, Xinghua People's Hospital Xinghua 225700, P. R. China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Xinghua People's Hospital Xinghua 225700, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Pan
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D001783:Blood Flow Velocity; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D017023:Coronary Angiography; D003326:Coronary Circulation / Q000187:drug effects*; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008297:Male; D017566:Microvascular Angina / Q000097:blood / Q000000981:diagnostic imaging / Q000208:ethnology / Q000235:genetics* / Q000503:physiopathology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Chinese; Slow coronary flow; coronary artery disease; genetic polymorphism; interleukin-10