title: Production of novel NaN3-resistant creatine amidinohydrolase in recombinant Escherichia coli.
abstract: Creatinase (creatine amidinohydrolase), an important medical enzyme, has been used for clinical diagnosis of renal function because of its high substrate specificity. Recently, we successfully cloned a NaN3-resistant creatinase encoding gene from Arthrobacter nicotianae. By optimizing the cultivation process, we realized its high-level expression in Escherichia coli. In this addendum, production of this NaN3-resistant creatinase in E. coli and future research were further discussed.
authors.affiliation: a The Key Laboratory of Industrial Biotechnology; Ministry of Education; Jiangnan University ; Wuxi , China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.forename: Zhen
authors.initials: Z
authors.lastname: Kang
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
mesh_terms: D001173:Arthrobacter / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003404:Creatinine / Q000032:analysis* / Q000737:chemistry; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007677:Kidney Function Tests; D016958:Pseudomonas putida / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D019810:Sodium Azide / Q000633:toxicity; D013379:Substrate Specificity; D014512:Ureohydrolases / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D019810:Sodium Azide; D003404:Creatinine; D014512:Ureohydrolases; C019944:creatinase
keywords: NaN3-resistance; arthrobacter nicotianae; creatinase; escherichia coli