title: [Antibiotic resistance and molecular characterization of Vibrio cholera strains isolated from an outbreak of cholera epidemic in Jiangsu province].
abstract: OBJECTIVE
  To assess the antibiotic resistance and molecular characterization of cholera strains and to provide basis for clinical treatment and prevention of cholera.
  
  
  METHODS
  4 stains isolated from an outbreak of cholera epidemic in Huai'an City in Jiangsu province in September 2010 were characterized using antibiotic susceptibility, biotype analysis, virluence genes detection, ctxB gene sequencing, and PFGE analysis.
  
  
  RESULTS
  The 4 strains were all resistant to sulphamethoxazole/trimethoprim, erythromycin, streptomycin. High drug susceptibility of the samples was found to 6 kinds of antibiotics such as amikacin, norfloxacin, ciprofloxacin, gentamicin, chloramphenicol, ampicillin. The isolates expressed phenotypic traits of both serogroup O1 ogawa and El Tor and carried 9 kinds of virulence genes, ctxA, ace, zot, toxR, tcpI, ompU, rtxC, tcpA, and hlyA gene. They were also identified as harboring the classical ctxB genotype based on amino acid residue substitutions. The PFGE profiles of NotI showed a single banding pattern, while SfiI's was 2 banding patterns.
  
  
  CONCLUSIONS
  The bacterium type of Vibrio cholerae causing the epidemic outbreak of cholera belonged to the atypical EL Tor variant which was also identified as toxicogenic strain. The mapping of the strains prompted that there should be the common contamination source. Drug sensitivity test can guide the clinical drug use, in order to reduce the emergence of resistant strains.
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Dong
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Bao
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Qian
authors.affiliation: Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health/ Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China. Email: tfyepi@163.com.
authors.forename: Fenyang
authors.initials: F
authors.lastname: Tang
mesh_terms: D000900:Anti-Bacterial Agents; D002771:Cholera; D002772:Cholera Toxin; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D004352:Drug Resistance, Microbial; D058872:Epidemics; D005838:Genotype; D006801:Humans; D014734:Vibrio cholerae; D041241:Vibrio cholerae O1; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D002772:Cholera Toxin