title: Chemical constituents of Swertia delavayi and their anti-hepatitis B virus activity.
abstract: Fifteen known compounds were isolated from Swertia delavayi by silica gel, Sephadex LH-20 and Rp-18 column chromatographies. Based on extensive spectroscopic analysis (MS, 1H, 13C-NMR), their structures were identified aserythrocentaurin (1), erythrocentaurindimethylacetal (2), sweroside (3), swertiamarin (4), gentiopicroside (5), swertiakoside A (6), 2'-O-acetylswertiamarin (7), 4'-O-[(Z) -coumaroyl] swertiamarin (8), 1,5,8-trihydroxy-3-methoxyxanthone (9), 8-O-β-D-glucopyranosyl-1-hydroxy-2,3, 5-trimethoxyxanthone (10), 8-O-[β-D-xyl- opyranosyl-(1 --> 6)-β-D-glucopyranosyl]-7,8-dihydroxy-3-methoxyxanthone (11), isovitexin (12), β-sitosterol (13), daucosterol (14), and oleanolic acid (15). Among them, ten ones (14, 7-11, 13) were obtained from S. delavayi for the first time. The isolates were evaluated for their anti-HBV activities in HepG 2. 2. 15 cell line in vitro. The results showed that compound 1, 2, 6, 7, 9 and 12 exhibited significant inhibitory activity on HBV DNA replication with IC50 values from 0.05 to 1.46 mmol x L(-1).
authors.forename: Tuan-wu
authors.initials: TW
authors.lastname: Cao
authors.forename: Chang-an
authors.initials: CA
authors.lastname: Geng
authors.forename: Yun-bao
authors.initials: YB
authors.lastname: Ma
authors.forename: Kang
authors.initials: K
authors.lastname: He
authors.forename: Ning-jia
authors.initials: NJ
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Ji-jun
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D008279:Magnetic Resonance Imaging; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D029967:Swertia / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal