title: In Vivo Nanodetoxication for Acute Uranium Exposure.
abstract: Accidental exposure to uranium is a matter of concern, as U(VI) is nephrotoxic in both human and animal models, and its toxicity is associated to chemical toxicity instead of radioactivity. We synthesized different PAMAM G4 and G5 derivatives in order to prove their interaction with uranium and their effect on the viability of red blood cells in vitro. Furthermore, we prove the effectiveness of the selected dendrimers in an animal model of acute uranium intoxication. The dendrimer PAMAM G4-Lys-Fmoc-Cbz demonstrated the ability to chelate the uranyl ion in vivo, improving the biochemical and histopathologic features caused by acute intoxication with uranium.
authors.affiliation: Laboratory of Asymmetric Synthesis, Institute of Chemistry and Natural Resources, University of Talca, P.O. Box 747, Talca 3460000, Maule, Chile. fmanke@utalca.cl.|Nanobiotechnology Division at Talca University, Fraunhofer Chile Research Foundation-Center for Systems Biotechnology, FCR-CSB, P.O. Box 747, Talca 3460000, Maule, Chile. fmanke@utalca.cl.|Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, University of Talca, P.O. Box 747, Talca 3460000, Maule, Chile. fmanke@utalca.cl.
authors.forename: Luis
authors.initials: L
authors.lastname: Guzmán
authors.affiliation: Laboratory of Asymmetric Synthesis, Institute of Chemistry and Natural Resources, University of Talca, P.O. Box 747, Talca 3460000, Maule, Chile. fmanke@utalca.cl.|Nanobiotechnology Division at Talca University, Fraunhofer Chile Research Foundation-Center for Systems Biotechnology, FCR-CSB, P.O. Box 747, Talca 3460000, Maule, Chile. fmanke@utalca.cl.
authors.forename: Esteban F
authors.initials: EF
authors.lastname: Durán-Lara
authors.affiliation: Laboratory of Asymmetric Synthesis, Institute of Chemistry and Natural Resources, University of Talca, P.O. Box 747, Talca 3460000, Maule, Chile. wdonoso@utalca.cl.
authors.forename: Wendy
authors.initials: W
authors.lastname: Donoso
authors.affiliation: Nanobiotechnology Division at Talca University, Fraunhofer Chile Research Foundation-Center for Systems Biotechnology, FCR-CSB, P.O. Box 747, Talca 3460000, Maule, Chile. fmanke@utalca.cl.
authors.forename: Fabiane M
authors.initials: FM
authors.lastname: Nachtigall
authors.affiliation: Laboratory of Asymmetric Synthesis, Institute of Chemistry and Natural Resources, University of Talca, P.O. Box 747, Talca 3460000, Maule, Chile. lssantos@utalca.cl.|Nanobiotechnology Division at Talca University, Fraunhofer Chile Research Foundation-Center for Systems Biotechnology, FCR-CSB, P.O. Box 747, Talca 3460000, Maule, Chile. lssantos@utalca.cl.
authors.forename: Leonardo S
authors.initials: LS
authors.lastname: Santos
mesh_terms: D054508:Acute Radiation Syndrome / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D015415:Biomarkers; D002614:Chelating Agents / Q000008:administration & dosage* / Q000737:chemistry; D050091:Dendrimers / Q000008:administration & dosage / Q000737:chemistry; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D053758:Nanoparticles; D009757:Nylons / Q000737:chemistry; D000069079:Radiation Exposure / Q000009:adverse effects*; D014501:Uranium / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D002614:Chelating Agents; D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; D014501:Uranium
keywords: PAMAM dendrimer derivatives; acute intoxication; chelating agent; uranium