title: Novel surface markers directed against adult human gallbladder.
abstract: Novel cell surface-reactive monoclonal antibodies generated against extrahepatic biliary cells were developed for the isolation and characterization of different cell subsets from normal adult human gallbladder. Eleven antigenically distinct gallbladder subpopulations were isolated by fluorescence-activated cell sorting. They were classified into epithelial, mesenchymal, and pancreatobiliary (PDX1(+)SOX9(+)) subsets based on gene expression profiling. These antigenically distinct human gallbladder cell subsets could potentially also reflect different functional properties in regards to bile physiology, cell renewal and plasticity. Three of the novel monoclonal antibodies differentially labeled archival sections of primary carcinoma of human gallbladder relative to normal tissue. The novel monoclonal antibodies described herein enable the identification and characterization of antigenically diverse cell subsets within adult human gallbladder and are putative tumor biomarkers.
authors.affiliation: Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA. Electronic address: galivo@ohsu.edu.
authors.forename: Feorillo H
authors.initials: FH
authors.lastname: Galivo
authors.affiliation: Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Dorrell
authors.affiliation: Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Grompe
authors.affiliation: Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: YongPing
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Streeter
authors.affiliation: Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA. Electronic address: grompem@ohsu.edu.
authors.forename: Markus
authors.initials: M
authors.lastname: Grompe
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology; D017734:Bile Ducts, Extrahepatic / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D045744:Cell Line, Tumor; D002469:Cell Separation; D003549:Cystic Duct / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005704:Gallbladder / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D008648:Mesoderm / Q000166:cytology; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D015415:Biomarkers