title: Rapid phenotypic fingerprinting of cell products by robust measurement of ubiquitous surface markers.
abstract: Flow cytometry is one of the most versatile and powerful approach for the quantitative analysis of cell suspensions. With widespread applications in basic and clinical research, its commonest use is in the detection of cell populations labelled against markers specific for a particular phenotype. In this study, we aimed to expand the potential of flow cytometry by describing a method based on robust automated quantification of ubiquitous cell surface markers. We demonstrate that automatic fluorescence standardization combined with whole cell population analysis yields highly reproducible results and can alleviate many of the difficulties associated with conventional analyses. This new generic approach is very flexible for quantifying the uniqueness of entire cell populations regardless of their composition. It provides quantitative phenotypic fingerprints rapidly, does not incorporate subjective factors, is more amenable to standardization, and is easily transferable across a wide diversity of applications, such as quality control for cell manufacture and authentication of cell products.
authors.affiliation: Cell Therapy Catapult, Guy's Hospital, Great Maze Pond, London, SE1 9RT, United Kingdom.
authors.forename: Marc-Olivier
authors.initials: MO
authors.lastname: Baradez
authors.affiliation: LGC, Science and Technology, Teddington, Middlesex, TW11 0LY, United Kingdom.
authors.forename: Tamara
authors.initials: T
authors.lastname: Lekishvili
authors.affiliation: Cell Therapy Catapult, Guy's Hospital, Great Maze Pond, London, SE1 9RT, United Kingdom.
authors.forename: Damian
authors.initials: D
authors.lastname: Marshall
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D051927:CD24 Antigen / Q000032:analysis / Q000276:immunology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods*; D027261:Fusion Regulatory Protein-1 / Q000032:analysis / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis / Q000276:immunology*; D016130:Immunophenotyping / Q000379:methods; D025341:Principal Component Analysis
chemical_list: D015415:Biomarkers; D051927:CD24 Antigen; D027261:Fusion Regulatory Protein-1; D018960:Hyaluronan Receptors
keywords: cell authentication; cell manufacture; cell therapy; fingerprinting; flow cytometry; immunophenotyping; normalization