title: NKD1 down-regulation is associated with poor prognosis in breast invasive ductal carcinoma.
abstract: As a negative modulator of the canonical Wnt signaling pathway, Naked1 (NKD1) is widely expressed in many normal tissues. However, the expression and clinicopathological significance of NKD1 in patients with breast cancer is still unclear. The aim of this study was to evaluate NKD1 expression in breast cancer and to investigate the question of whether reduced expression of NKD1 may have any pathological significance in breast cancer development or progression. In this study, we performed western blotting and immunohistochemistry to evaluate the expression of NKD1 and relevance with clinicopathological factors in the breast invasive ductal carcinoma. Reduction of NKD1 was significantly correlated with lymph node metastasis, histological grade and ER expression in breast cancer. Patients with negative NKD1 expression had significantly lower cumulative postoperative 5 year survival rate than those with positive NKD1 expression. This interpretation is in keeping with the results obtained from our in vitro experiments on MDA-MB-231 cells, we demonstrated that upregulation of NKD1 expression by infect with an adenovirus containing a NKD1 vector significantly reduced the migration of breast cancer cells. These data suggest that NKD1 plays an important role in invasion in human breast cancer and it appears to be a potential prognostic marker for patients with breast cancer.
authors.affiliation: Department of Breast Surgery, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Lv
authors.affiliation: Department of Breast Surgery, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Central Laboratory of Molecular Biology, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Liu
authors.affiliation: Department of Cerebrovascular Disease Research Institute, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Jin
authors.affiliation: Department of Pediatric Surgery, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Breast Surgery, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Fu-Nian
authors.initials: FN
authors.lastname: Li
authors.affiliation: Department of Breast Surgery, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Department of Breast Surgery, The Affiliated Hospital of Qingdao University Qingdao 266003, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D002135:Calcium-Binding Proteins; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D011960:Receptors, Estrogen / Q000378:metabolism; D012307:Risk Factors; D013997:Time Factors; D014162:Transfection; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; C449091:NKD1 protein, human; D011960:Receptors, Estrogen
keywords: Breast carcinoma; NKD1; metastasis; prognostic marker