title: Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by the γ-Ray Irradiation Technique.
abstract: The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the γ-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the γ-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP-2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and the mRNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Young-Pil
authors.identifier: 0000-0003-2466-797X
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu 136-701, Republic of Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Molecular & Cell Biology, University of California, 142 LSA No. 3200, Berkeley, CA 94720-3200, USA.
authors.forename: Won Jae
authors.initials: WJ
authors.lastname: Jeong
authors.affiliation: Division of Bio-Imaging Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Hak-Jun
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Jae-Jun
authors.identifier: 0000-0002-8488-9091
authors.initials: JJ
authors.lastname: Song
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Hae-Ryong
authors.initials: HR
authors.lastname: Song
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000528:radiation effects*; D049109:Cell Proliferation / Q000235:genetics / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D010012:Osteogenesis / Q000235:genetics*; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011091:Polyesters; C016240:polycaprolactone; D000469:Alkaline Phosphatase