title: [mRNA Expression Analysis of 10 Reproduction-related Genes in Different Developmental Proglottids of Moniezia expansa].
abstract: According to the results of transcriptome sequencing in Moniezia expansa, 10 functional genes which represent different expression patterns in different developmental proglottids were selected, including KIFC1, Kif17, tgf-beta, SmadD, tgf-beta receptor, HSD5, aminopeptidase puromycin, Methionine aminopeptidase 2, transcription factor fork head and Sox transcription factor. A real-time fluorescent quantitative PCR assay was developed for detection of mRNA expression level of these taget genes with its beta-tubulin as an internal control. The results showed that compared to scolex-neck proglottids, the mRNA levels of 2 genes (KIFC1, Kif17) in immature proglottides, 4 genes (KIFC1, Kif17, tgf-beta receptor, and amniopeptidase puromycin) in mature proglottides, and 3 genes (HSD5, tgf-beta receptor, and Methionine aminopeptidase 2) in gravid proglottides were up-regulated (P < 0.01).
authors.forename: Zheng-rong
authors.initials: ZR
authors.lastname: Wang
authors.forename: Xin-wen
authors.initials: XW
authors.lastname: Bo
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D002589:Cestoda / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger