title: A Comparison of Anammox Bacterial Abundance and Community Structures in Three Different Emerged Plants-Related Sediments.
abstract: Quantitative polymerase chain reaction (qPCR) assays and 16S rRNA gene clone libraries were used to document the abundance, diversity and community structure of anaerobic ammonia-oxidising (anammox) bacteria in the rhizosphere and non-rhizosphere sediments of three emergent macrophyte species (Iris pseudacorus, Thalia dealbata and Typha orientalis). The qPCR results confirmed the existence of anammox bacteria (AMX) with observed log number of gene copies per dry gram sediment ranging from 5.00 to 6.78. AMX was more abundant in T. orientalis-associated sediments than in the other two plant species. The I. pseudacorus- and T. orientalis-associated sediments had higher Shannon diversity values, indicating higher AMX diversity in these sediments. Based on the 16S rRNA gene, Candidatus 'Brocadia', Candidatus 'Kuenenia', Candidatus 'Jettenia' and new clusters were observed with the predominant Candidatus 'Kuenenia' cluster. The I. pseudacorus-associated sediments contained all the sequences of the C. 'Jettenia' cluster. Sequences obtained from T. orientalis-associated sediments contributed more than 90 % sequences in the new cluster, whereas none was found from I. pseudacorus. The new cluster was distantly related to known sequences; thus, this cluster was grouped outside the known clusters, indicating that the new cluster may be a new Planctomycetales genus. Further studies should be undertaken to confirm this finding.
authors.affiliation: Department of Environment Engineering, School of the Environment and Safety Engineering, Jiangsu University, 301, Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China, chujinyu88@163.com.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Chu
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
authors.forename: Yimin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D058448:Biota; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007498:Iris / Q000382:microbiology; D032426:Marantaceae / Q000382:microbiology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D032401:Typhaceae / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000641:Ammonia