title: The Atypical Cadherin Dachsous Controls Left-Right Asymmetry in Drosophila.
abstract: Left-right (LR) asymmetry is essential for organ development and function in metazoans, but how initial LR cue is relayed to tissues still remains unclear. Here, we propose a mechanism by which the Drosophila LR determinant Myosin ID (MyoID) transfers LR information to neighboring cells through the planar cell polarity (PCP) atypical cadherin Dachsous (Ds). Molecular interaction between MyoID and Ds in a specific LR organizer controls dextral cell polarity of adjoining hindgut progenitors and is required for organ looping in adults. Loss of Ds blocks hindgut tissue polarization and looping, indicating that Ds is a crucial factor for both LR cue transmission and asymmetric morphogenesis. We further show that the Ds/Fat and Frizzled PCP pathways are required for the spreading of LR asymmetry throughout the hindgut progenitor tissue. These results identify a direct functional coupling between the LR determinant MyoID and PCP, essential for non-autonomous propagation of early LR asymmetry.
authors.affiliation: Institut de Biologie Valrose, University of Nice Sophia Antipolis, 06108 Nice, France; Institut de Biologie Valrose, CNRS, UMR 7277, 06108 Nice, France; Institut de Biologie Valrose, INSERM, U1091, 06108 Nice, France.
authors.forename: Nicanor
authors.initials: N
authors.lastname: González-Morales
authors.affiliation: Institut de Biologie Valrose, University of Nice Sophia Antipolis, 06108 Nice, France; Institut de Biologie Valrose, CNRS, UMR 7277, 06108 Nice, France; Institut de Biologie Valrose, INSERM, U1091, 06108 Nice, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Géminard
authors.affiliation: Institut de Biologie Valrose, University of Nice Sophia Antipolis, 06108 Nice, France; Institut de Biologie Valrose, CNRS, UMR 7277, 06108 Nice, France; Institut de Biologie Valrose, INSERM, U1091, 06108 Nice, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Lebreton
authors.affiliation: Institut de Biologie Valrose, University of Nice Sophia Antipolis, 06108 Nice, France; Institut de Biologie Valrose, CNRS, UMR 7277, 06108 Nice, France; Institut de Biologie Valrose, INSERM, U1091, 06108 Nice, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Cerezo
authors.affiliation: Institut de Biologie Valrose, University of Nice Sophia Antipolis, 06108 Nice, France; Institut de Biologie Valrose, CNRS, UMR 7277, 06108 Nice, France; Institut de Biologie Valrose, INSERM, U1091, 06108 Nice, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Coutelis
authors.affiliation: Institut de Biologie Valrose, University of Nice Sophia Antipolis, 06108 Nice, France; Institut de Biologie Valrose, CNRS, UMR 7277, 06108 Nice, France; Institut de Biologie Valrose, INSERM, U1091, 06108 Nice, France. Electronic address: noselli@unice.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Noselli
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D015820:Cadherins / Q000235:genetics / Q000502:physiology*; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D004064:Digestive System / Q000166:cytology / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D017344:Genes, Insect; D008954:Models, Biological; D009218:Myosins / Q000235:genetics / Q000502:physiology
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; C094449:ds protein, Drosophila; D009218:Myosins