title: A Direct Sulfation Process of a Marine Polysaccharide in Ionic Liquid.
abstract: GY785 is an exopolysaccharide produced by a mesophilic bacterial strain Alteromonas infernus discovered in the deep-sea hydrothermal vents. GY785 highly sulfated derivative (GY785 DRS) was previously demonstrated to be a promising molecule driving the efficient mesenchymal stem cell chondrogenesis for cartilage repair. This glycosaminoglycan- (GAG-) like compound was modified in a classical solvent (N,N'-dimethylformamide). However, the use of classical solvents limits the polysaccharide solubility and causes the backbone degradation. In the present study, a one-step efficient sulfation process devoid of side effects (e.g., polysaccharide depolymerization and/or degradation) was developed to produce GAG-like derivatives. The sulfation of GY785 derivative (GY785 DR) was carried out using ionic liquid as a reaction medium. The successful sulfation of this anionic and highly branched heteropolysaccharide performed in ionic liquid would facilitate the production of new molecules of high specificity for biological targets such as tissue engineering or regenerative medicine.
authors.affiliation: IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, rue de l'île d'Yeu, BP 21105, 44311 Nantes Cedex 03, France ; Institut des Matériaux Jean Rouxel (IMN), Université de Nantes, CNRS, 2 rue de la Houssinière, BP 32229, 44322 Nantes Cedex 3, France.
authors.forename: Nathalie
authors.identifier: 0000-0003-1598-2185
authors.initials: N
authors.lastname: Chopin
authors.affiliation: IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, rue de l'île d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sinquin
authors.affiliation: IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, rue de l'île d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Ratiskol
authors.affiliation: IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, rue de l'île d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.
authors.forename: Agata
authors.identifier: 0000-0001-7093-3832
authors.initials: A
authors.lastname: Zykwinska
authors.affiliation: INSERM U791, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire (LIOAD), Université de Nantes, 1 place Alexis Ricordeau, BP 84215, 44042 Nantes Cedex 1, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.affiliation: Laboratoire de Résonance Magnétique Nucléaire, Université de Bretagne Occidentale, CS 93837, 29238 Brest Cedex 3, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cérantola
authors.affiliation: Institut des Matériaux Jean Rouxel (IMN), Université de Nantes, CNRS, 2 rue de la Houssinière, BP 32229, 44322 Nantes Cedex 3, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Le Bideau
authors.affiliation: IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, rue de l'île d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.
authors.forename: Sylvia
authors.identifier: 0000-0002-2449-5410
authors.initials: S
authors.lastname: Colliec-Jouault
mesh_terms: D020591:Alteromonas / Q000737:chemistry; D059001:Aquatic Organisms / Q000737:chemistry; D020219:Chondrogenesis / Q000187:drug effects*; D052578:Ionic Liquids / Q000008:administration & dosage* / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D044968:Regenerative Medicine; D013431:Sulfates / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D052578:Ionic Liquids; D011134:Polysaccharides; D013431:Sulfates