title: Eugenol nanocapsule for enhanced therapeutic activity against periodontal infections.
abstract: Eugenol is a godsend to dental care due to its analgesic, local anesthetic, and anti-inflammatory and antibacterial effects. The aim of the present research work was to prepare, characterize and evaluate eugenol-loaded nanocapsules (NCs) against periodontal infections. Eugenol-loaded polycaprolactone (PCL) NCs were prepared by solvent displacement method. The nanometric size of the prepared NCs was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The in vitro drug release was found to follow a biphasic pattern and followed Michaelis-Menten like model. The percentage cell viability values near to 100 in the cell viability assay indicated that the NCs are not cytotoxic. In the in vivo studies, the eugenol NC group displayed significant difference in the continuity of epithelium of the interdental papilla in comparison to the untreated, pure eugenol and placebo groups. The in vivo performance of the eugenol-loaded NCs using ligature-induced periodontitis model in rats indicated that eugenol-loaded NCs could prevent septal bone resorption in periodontitis. On the basis of our research findings it could be concluded that eugenol-loaded PCL NCs could serve as a novel colloidal drug delivery system for enhanced therapeutic activity of eugenol in the treatment of periodontal infections.
authors.affiliation: a Department of Pharmaceutics, Faculty of Pharmacy , Jamia Hamdard, Hamdard Nagar , New Delhi , India .
authors.forename: Kannissery
authors.initials: K
authors.lastname: Pramod
authors.affiliation: b Centre for Biomedical Engineering, Indian Institute of Technology (IIT) , Hauz Khas , New Delhi , India .
authors.forename: M R
authors.initials: MR
authors.lastname: Aji Alex
authors.affiliation: c Department of Biotechnology , Jaypee Institute of Information Technology , Noida , India , and.
authors.forename: Manisha
authors.initials: M
authors.lastname: Singh
authors.affiliation: c Department of Biotechnology , Jaypee Institute of Information Technology , Noida , India , and.
authors.forename: Shweta
authors.initials: S
authors.lastname: Dang
authors.affiliation: d Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy , Jamia Hamdard, Hamdard Nagar , New Delhi , India.
authors.forename: Shahid H
authors.initials: SH
authors.lastname: Ansari
authors.affiliation: a Department of Pharmaceutics, Faculty of Pharmacy , Jamia Hamdard, Hamdard Nagar , New Delhi , India .
authors.forename: Javed
authors.initials: J
authors.lastname: Ali
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001424:Bacterial Infections / Q000188:drug therapy*; D002470:Cell Survival; D065546:Drug Liberation; D005054:Eugenol / Q000008:administration & dosage* / Q000494:pharmacology*; D005882:Gingival Diseases / Q000188:drug therapy*; D008297:Male; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D053769:Nanocapsules; D011091:Polyesters; C016240:polycaprolactone; D005054:Eugenol
keywords: Cell viability assay; nanoencapsulation; polycaprolactone; solvent displacement method