title: Cell-Mediated Hemolytic Activity of Nosocomial Pseudomonas Aeruginosa Strains.
abstract: Cell-mediated hemolysis and adhesion index of nosocomial P. aeruginosa strains were experimentally studied. The highest hemoglobin release was recorded after centrifugation of erythrocyte and bacterial cell suspension preincubated at 37 C. All cultures were referred to highly adherent variants. The relationship between P. aeruginosa adhesion activity and erythrocyte lysis was found only in "passive" cell-cell contact. No correlation between cell-associated hemolysis and hemolysis caused by secreted factors was detected. It seems that the cytotoxicity of the studied P. aeruginosa strains was determined by ExoU and ExoS third type secretion effectors.
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, Perm, Russia, mar@iegm.ru.
authors.forename: M V
authors.initials: MV
authors.lastname: Kuznetsova
authors.forename: T I
authors.initials: TI
authors.lastname: Karpunina
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism / Q000633:toxicity; D001426:Bacterial Proteins / Q000378:metabolism / Q000633:toxicity; D001427:Bacterial Toxins / Q000378:metabolism / Q000633:toxicity; D002448:Cell Adhesion / Q000502:physiology*; D002498:Centrifugation; D003428:Cross Infection / Q000503:physiopathology*; D017931:DNA Primers / Q000235:genetics; D004912:Erythrocytes / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000503:physiopathology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D006454:Hemoglobins; C111749:pseudomonas exoprotein A protein, Pseudomonas aeruginosa; D036002:ADP Ribose Transferases; C444820:exoenzyme S