title: Application of a highly sensitive magnetic solid phase extraction for phytochemical compounds in medicinal plant and biological fluids by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.
abstract: A highly sensitive method using reduced graphene oxide with iron oxide (rGO/Fe3 O4 ) as the sorbent in magnetic SPE has been developed for the purification of five anthraquinones (emodin, rhein, aloeemodin, physcion, and chrysophanol) in rhubarb and rat urine by ultra-HPLC coupled with quadrupole TOF/MS. The extraction was accomplished by adding trace amount rGO/Fe3 O4 suspension to 200 mL of aqueous mixture, and the excellent adsorption capacity of the nanoparticles was fully demonstrated in this procedure. Under the optimized conditions, the calibration curves were linear in the concentration range of 0.05-27.77 ng/mL with correlation coefficients varying from 0.9902 to 0.9978. The LODs ranged from 0.28 to 58.99 pg/mL. The experimental results indicated that the proposed method was feasible for the analysis of anthraquinones in rhubarb and urine samples.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Ohio State University, Columbus, OH, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Yi
authors.affiliation: Department of Traditional Chinese Medicine, Hangzhou Red Cross Hospital, Hangzhou, P. R. China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Hu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Peng
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Qiong-Yao
authors.initials: QY
authors.lastname: Zhu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D064209:Phytochemicals / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; D058185:Magnetite Nanoparticles; D064209:Phytochemicals
keywords: Anthraquinones; Magnetic SPE; Rat urine; Rhubarb; Ultra-HPLC coupled with quadrupole TOF/MS