title: Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents.
abstract: We observed the relationship between lifespan and mitochondria, including antioxidant systems, ultrastructure, and the hydrogen peroxide and malondialdehyde contents in 4 h imbibed oat (Avena sativa L.) seeds that were aged with different moisture contents (4%, 10% and 16%) for 0 (the control), 8, 16, 24, 32 and 40 d at 45 °C. The results showed that the decline in the oat seed vigor and in the integrity of the mitochondrial ultrastructure occurred during the aging process, and that these changes were enhanced by higher moisture contents. Mitochondrial antioxidants in imbibed oat seeds aged with a 4% moisture content were maintained at higher levels than imbibed oat seeds aged with a 10% and 16% moisture content. These results indicated that the levels of mitochondrial antioxidants and malondialdehyde after imbibition were related to the integrity of the mitochondrial membrane in aged oat seeds. The scavenging role of mitochondrial superoxide dismutase was inhibited in imbibed oat seeds aged at the early stage. Monodehydroascorbate reductase and dehydroascorbate reductase played more important roles than glutathione reductase in ascorbate regeneration in aged oat seeds during imbibition.
authors.affiliation: Forage Seed Lab, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China.
authors.forename: Fangshan
authors.initials: F
authors.lastname: Xia
authors.affiliation: Forage Seed Lab, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China.
authors.forename: Xianguo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Forage Seed Lab, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China.
authors.forename: Manli
authors.initials: M
authors.lastname: Li
authors.affiliation: Forage Seed Lab, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China. Electronic address: maopeisheng@hotmail.com.
authors.forename: Peisheng
authors.initials: P
authors.lastname: Mao
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D018554:Avena / Q000378:metabolism / Q000648:ultrastructure; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000378:metabolism / Q000648:ultrastructure; D012639:Seeds / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000975:Antioxidants
keywords: Antioxidant systems; Mitochondrial structure; Oat; Seed aging