title: Effects of recombinant lycopene dietary supplement on the egg quality and blood characteristics of laying quails.
abstract: This study was conducted to determine the effect of dietary supplement of bacterial lycopene (BL) produced by Escherichia coli on the egg quality and blood characteristics of laying quails. The antioxidant activity measurement showed that BL exhibited 100% 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity at a concentration of 4.65 μg/ml, which was more effective than butylated hydroxytoluene (BHT) and commercial lycopene (CL). Moreover, seven dietary groups of laying quails consisting of 10 100-day-old quails (Coturnix coturnix japonica) each were fed with the basal diet supplemented with BL, CL or canthaxanthin (CA) for 4 weeks. Consequently, the triglyceride content of yolk was significantly lower in the group with BL and CL supplement. The serum malondialdehyde (MDA) level of the BL- and CA-supplemented groups at 18 mg/kg was lower than the control group. In conclusion, BL has a high antioxidant activity and is promising as a feed additive in the diet of laying quails.
authors.affiliation: Department of Animal Science, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Hsu
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan.
authors.forename: Chung-Jen
authors.initials: CJ
authors.lastname: Chiang
authors.affiliation: Department of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan; School of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Chao
authors.affiliation: Department of Animal Science, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.
authors.forename: Chi-Huan
authors.initials: CH
authors.lastname: Chang
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
authors.forename: Li-Jen
authors.initials: LJ
authors.lastname: Lin
authors.affiliation: Department of Animal Science, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.
authors.forename: Bi
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Animal Science, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan. Electronic address: ttlee@dragon.nchu.edu.tw.
authors.forename: Tzu-Tai
authors.initials: TT
authors.lastname: Lee
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000378:metabolism; D002338:Carotenoids / Q000008:administration & dosage / Q000494:pharmacology*; D003370:Coturnix / Q000097:blood* / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004530:Egg Yolk / Q000737:chemistry / Q000187:drug effects*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D000077276:Lycopene; D008315:Malondialdehyde / Q000097:blood; D010058:Oviposition / Q000187:drug effects / Q000502:physiology; D010851:Picrates / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D014280:Triglycerides; D002338:Carotenoids; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D000077276:Lycopene
keywords: Antioxidant activity; Egg quality; Feed additive; Laying quail; Recombinant lycopene