title: Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using Illumina sequencing.
abstract: OBJECTIVE
  Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon.
  
  
  RESULTS
  Nineteen microsatellite loci were identified from an Illumina paired-end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ∼80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380.
  
  
  CONCLUSIONS
  These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.
authors.affiliation: Plant Biology Department, University of Georgia, Athens, Georgia 30602 USA.
authors.forename: Dorset W
authors.initials: DW
authors.lastname: Trapnell
authors.affiliation: Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA.
authors.forename: Rochelle R
authors.initials: RR
authors.lastname: Beasley
authors.affiliation: Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA.
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Lance
authors.affiliation: Queensland Herbarium, Department of Science, Information Technology and Innovation, Toowong, Queensland 4066, Australia ; Australian Tropical Herbarium, James Cook University, Smithfield, Queensland 4878, Australia.
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Field
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045 USA.
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Jones
keywords: Dendrobium calamiforme; Dockrillia calamiformis; Orchidaceae; genetic diversity; phylogeography; simple sequence repeat (SSR) markers