title: Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.
abstract: Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.
authors.affiliation: Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: O'Shea
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Anesthesiology, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA, 02115, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Webber
authors.affiliation: InVivo Therapeutics Corporation, One Kendall Square Building 1400 East, Floor 4, Cambridge, MA, 02139, USA.
authors.forename: Alex A
authors.initials: AA
authors.lastname: Aimetti
authors.affiliation: Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004355:Drug Stability; D005612:Freeze Drying; D020100:Hydrogels / Q000737:chemistry*; D006860:Hydrogen Bonding; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014199:Trehalose / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D020100:Hydrogels; D014199:Trehalose
keywords: drug delivery; hydrogels; pharmaceutical excipients; protein stability; trehalose