title: Nucleotide modulates odor response through activation of purinergic receptor in olfactory sensory neuron.
abstract: Extracellular nucleotides are important neurotransmitters, neuromodulators and paracrine factors in the neural sensory system [16]. Most of purines and pyrimidines act on the associated purinergic cell-surface receptors to mediate sensory transduction and modulation. Previously, we reported a subgroup of heptaldehyde (H)/2-hepatanone (Ho)-responsive olfactory sensory neurons (H/Ho-OSNs) in the ventral endoturbinates [31]. Through the calcium image recording, we characterized that ATP elicited [Ca(2+)]i increase in the presence of extracellular calcium, while depletion of intracellular calcium stores blocked UTP-evoked [Ca(2+)]i increase. Pharmacological studies indicated that P2X3 was expressed in the H/Ho-OSNs, modulating both heptaldehyde (H) and 2-hepatanone (Ho)-induced responses. These data indicated that activation of purinergic receptor negatively modulated odor response, providing the evidence to support the possible protective effect of purinergic receptor in OSNs.
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, USA. Electronic address: yyu17@iit.edu.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009812:Odorants; D058913:Purinergic Agonists / Q000494:pharmacology; D011983:Receptors, Purinergic / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D014544:Uridine Triphosphate / Q000502:physiology*
chemical_list: D058913:Purinergic Agonists; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate
keywords: Calcium imaging; Nucleotide; Olfactory sensory neuron; Purinergic receptor