title: Fluorescent dyes as a reliable tool in P2X7 receptor-associated pore studies.
abstract: Since the nineteenth century, a great amount of different biological structures and processes have been assessed by fluorescent dyes. Along with the uses of these compounds as vital and histological dyes, some fluorescent dyes have become valuable tools for the study of the pore phenomenon in plasma membranes. Some ion channels capable of forming large conductance channels, such as P2X7, TRPV1, VDAC-1 and the maxi-anion channels transiently alter the plasma membrane permeability, producing pores, which permit the passage of molecules of up to 1,000 Da. In this review, we discuss the uses of the fluorescent dyes chosen in diverse studies of this topic up to now.
authors.affiliation: Laboratory of Cellular Communication, Oswaldo Cruz Foundation, Av. Brazil 4365, pavilion 108, room 28B Manguinhos, Rio de Janeiro, Brazil, CEP 21040-360, leonardobraga12@gmail.com.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Ferreira
authors.forename: Luíza
authors.initials: L
authors.lastname: Pereira
authors.forename: Robson
authors.initials: R
authors.lastname: Faria
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000378:metabolism*; D050995:Voltage-Dependent Anion Channel 1 / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; C546468:P2RX7 protein, human; D058486:Receptors, Purinergic P2X7; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; C494762:VDAC1 protein, human; D050995:Voltage-Dependent Anion Channel 1