title: Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.
abstract: DNA polymerase iota (Polι) can repair several types of DNA damage but has extremely low fidelity. Previous studies have shown an aberrantly elevated Polι expression in human esophageal squamous cell cancer tissues. However, there were few reports describing the role of Polι in esophageal cancer progression. Based on Real-time PCR assay, we found Polι expression was up-regulated in esophageal cancer tissues compared to adjacent normal tissues and overexpression of Polι was correlated to lymph node metastasis. Clonogenic assay and transwell chamber assay showed that overexpression of Polι had higher clongenic capability and invasive tendency in human esophageal squamous cell cancer cells. Expression of cyclin D1, an important cell cycle regulator, was found to be associated with that of Polι in tissue samples and cancer cells as analyzed by real-time PCR, immunohistochemistry, Western blotting and immunofluorescence assay. Flow cytometry analysis further showed that cell cycle distribution was altered in Polι overexpressing cells. These results indicated that expression of Polι correlates significantly with tumor proliferation and invasion. We conclude that Polι is involved in the degree of aggressiveness of human esophageal squamous cell cancer.
authors.affiliation: Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Haoyao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P.R. China.
authors.forename: Shitao
authors.initials: S
authors.lastname: Zou
authors.affiliation: School of Radiation Medicine and Protection, Medical College of Soochow University Suzhou 215123, Jiangsu, P. R. China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P.R. China.
authors.forename: Xingjun
authors.initials: X
authors.lastname: Meng
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P.R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Jinchang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China ; Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P.R. China.
authors.forename: Jundong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010641:Phenotype; D015854:Up-Regulation; D000094769:DNA Polymerase iota
chemical_list: D004259:DNA-Directed DNA Polymerase; D000094769:DNA Polymerase iota; C000722070:POLI protein, human
keywords: DNA polymerase iota; Esophageal cancer; cyclin D1; metastasis; proliferation