title: Characterization of the bay scallop (Argopecten irradians concentricus Say) transcriptome and identification of growth-related genes.
abstract: The bay scallop Argopecten irradians concentricus is a commercially important bivalve species for aquaculture in China. However, the scarcity of transcripotomic and genomic resources of the bay scallop has hindered the progress of genetic improvement of the animal. To change this situation, de novo transcriptome sequencing with Illumina HiSeq™2000 sequencing platform was carried out for the bay scallop. Through de novo assembly, 107,145 high quality unigenes were obtained. Totally, 44,901 and 34,673 unigenes were annotated in the Nr database and Swiss-Prot database, respectively. With gene ontology (GO) annotations, 77,834 (51.58%), 49,367 (32.72%) and 23,695 (15.70%) were involved in biological processes, cellular components and molecular functions, respectively. One hundred and thirty seven unigenes putatively involved in growth were identified through blastx. More than 2706 simple sequence repeats (SSRs) and 82,116 single nucleotide polymorphisms (SNPs) were also detected. These genes and markers may serve as new valuable resources for functional genomic and genetic studies on the scallop A. i. concentricus.
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Sigang
authors.initials: S
authors.lastname: Fan
authors.affiliation: Jimei University, Xiamen 361021, China.
authors.forename: Dongling
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Baosuo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Dahui
authors.initials: D
authors.lastname: Yu
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D049897:Pectinidae / Q000235:genetics*; D059467:Transcriptome
keywords: Argopecten irradians concentricus; Growth-related gene; SNP; SSR; Transcriptome