title: Significant association between rare IPO11-HTR1A variants and attention deficit hyperactivity disorder in Caucasians.
abstract: We comprehensively examined the rare variants in the IPO11-HTR1A region to explore their roles in neuropsychiatric disorders. Five hundred seventy-three to 1,181 rare SNPs in subjects of European descent and 1,234-2,529 SNPs in subjects of African descent (0 < minor allele frequency (MAF) < 0.05) were analyzed in a total of 49,268 subjects in 21 independent cohorts with 11 different neuropsychiatric disorders. Associations between rare variant constellations and diseases and associations between individual rare variants and diseases were tested. RNA expression changes of this region were also explored. We identified a rare variant constellation across the entire IPO11-HTR1A region that was associated with attention deficit hyperactivity disorder (ADHD) in Caucasians (T5: P = 7.9 × 10(-31) ; Fp: P = 1.3 × 10(-32) ), but not with any other disorder examined; association signals mainly came from IPO11 (T5: P = 3.6 × 10(-10) ; Fp: P = 3.2 × 1 0(-10) ) and the intergenic region between IPO11 and HTR1A (T5: P = 4.1 × 10(-30) ; Fp: P = 5.4 × 10(-32) ). One association between ADHD and an intergenic rare variant, i.e., rs10042956, exhibited region- and cohort-wide significance (P = 5.2 × 10(-6) ) and survived correction for false discovery rate (q = 0.006). Cis-eQTL analysis showed that, 29 among the 41 SNPs within or around IPO11 had replicable significant regulatory effects on IPO11 exon expression (1.5 × 10(-17) ≤P < 0.002) in human brain or peripheral blood mononuclear cell tissues. We concluded that IPO11-HTR1A was a significant risk gene region for ADHD in Caucasians.
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Zuo
authors.affiliation: Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, Colorado.
authors.forename: Laura
authors.initials: L
authors.lastname: Saba
authors.affiliation: Provincial Key Laboratory of Translational Cancer Medicine, Fujian Provincial Cancer Hospital, Fuzhou, Fujian, China.
authors.forename: Xiandong
authors.initials: X
authors.lastname: Lin
authors.affiliation: Biological Psychiatry Research Center, Beijing Huilongguan Hospital, Beijing, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Biostatistics and Epidemiology, College of Public Health, East Tennessee State University, Johnson City, Tennessee.
authors.forename: Kesheng
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut.|VA Alcohol Research Center, VA Cnnecticut Healthcare System, West Haven, Connecticut.
authors.forename: John H
authors.initials: JH
authors.lastname: Krystal
authors.affiliation: Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, Colorado.
authors.forename: Boris
authors.initials: B
authors.lastname: Tabakoff
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut.|Biological Psychiatry Research Center, Beijing Huilongguan Hospital, Beijing, China.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Luo
mesh_terms: D000293:Adolescent; D000328:Adult; D001741:Black or African American / Q000235:genetics; D001289:Attention Deficit Disorder with Hyperactivity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics*; D012307:Risk Factors; D044465:White People / Q000235:genetics*; D028961:beta Karyopherins / Q000235:genetics*
chemical_list: C583518:HTR1A protein, human; C475690:IPO11 protein, human; D028961:beta Karyopherins; D044282:Receptor, Serotonin, 5-HT1A
keywords: ADHD; IPO11; non-coding RNA; rare variant constellations