title: Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei.
abstract: Crustacean hyperglycemic hormone (CHH) is the most abundant neuropeptide produced by the X-organ/sinus gland (XO/SG) complex in the crustacean eyestalk. CHH plays a principal role in the control of glucose metabolism. The CHH-B1 isoform is produced in the eyestalk of Litopenaeus vannamei by alternative splicing of the chhB gene and its cDNA sequence has revealed that this isoform has a non-amidated C-terminal residue (CHH-like peptide). In this work, a recombinant CHH-B1 (rCHH-B1) with a sequence identical to the native hormone was expressed in the methylotrophic yeast Pichia pastoris X-33 and purified from the culture medium by RP-HPLC. The identity of the purified rCHH-B1 was confirmed by N-terminal sequencing and by using an anti-CHH-B1 polyclonal antibody. An in vivo assay showed that the hyperglycemic effect was dependant of the dosage of rCHH-B1, and the maximal hyperglycemic response was obtained with 250pmol treatment. These results suggest that the amino acid sequence of the C-terminus and its correct structure are both important for the hyperglycemic activity of naturally occurring non-amidated CHH peptides, such as CHH-B1. CHH-B1 appears to be the first reported CHH-like peptide with significant hyperglycemic activity produced in the sinus gland of a penaeid shrimp.
authors.affiliation: Laboratorio de Biología Celular y Molecular, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México.
authors.forename: Laura
authors.initials: L
authors.lastname: Camacho-Jiménez
authors.affiliation: Laboratorio de Biología Celular y Molecular, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México.
authors.forename: Edna
authors.initials: E
authors.lastname: Sánchez-Castrejón
authors.affiliation: Laboratorio de Biología Celular y Molecular, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México. Electronic address: eponce@cicese.mx.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ponce-Rivas
authors.affiliation: Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California (UABC), Av. Tecnológico s/n, Mesa de Otay, Tijuana, Baja California C.P. 22390, México.
authors.forename: Ma Enriqueta
authors.initials: ME
authors.lastname: Muñoz-Márquez
authors.affiliation: Laboratorio de Neurofarmacología Marina, Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM), Blvd. Juriquilla 3001, Juriquilla, Querétaro C.P. 76230, México.
authors.forename: Manuel B
authors.initials: MB
authors.lastname: Aguilar
authors.affiliation: Laboratorio de Ecofisiología de Organismos Acuáticos, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México.
authors.forename: Ana Denisse
authors.initials: AD
authors.lastname: Re
authors.affiliation: Laboratorio de Ecofisiología de Organismos Acuáticos, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México.
authors.forename: Fernando
authors.initials: F
authors.lastname: Díaz
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000494:pharmacology*; D007447:Invertebrate Hormones / Q000235:genetics / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000494:pharmacology*; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology
chemical_list: D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C015947:hyperglycemic hormone, crustacean
keywords: Crustacean hyperglycemic hormone; Crustacean neurohormone; Dose–response hyperglycemic analysis; Litopenaeus vannamei; Recombinant expression