title: MicroRNA-219-5p exerts tumor suppressor function by targeting ROBO1 in glioblastoma.
abstract: Previous studies have shown that miR-219-5p is dysregulated and exerts tumor-suppressive effects in cancer development and progression. However, the molecular function and mechanism of miR-219-5p in glioblastoma growth and invasion are still unclear. In the present study, we show that miR-219-5p was downregulated in a panel of glioma tissues with different grades and in all the human glioma cell lines examined. Ectopic expression of miR-219-5p inhibited proliferation and invasion and induced apoptosis in vitro, and xenograft formation in vivo. ROBO1 was found to be a direct target of miR-219-5p, and when overexpressed in miR-219-5p-expressing glioma cells, was able to restore proliferative and invasive ability. Finally, in vivo investigation confirmed that miR-219-5p was a tumor suppressor that regulated ROBO1 expression. Taken together, these studies demonstrate that miR-219-5p inhibited cancer cell growth and invasion by direct targeting ROBO1, implicating miR-219-5p as an attractive candidate for cancer therapy.
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Dalian Medical University, No. 467 Zhongshan Road, Dalian, Liaoning, 116027, China. zhuxufifa2007@163.com.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Dalian Medical University, No. 467 Zhongshan Road, Dalian, Liaoning, 116027, China. Lin_yin1@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Dalian Medical University, No. 467 Zhongshan Road, Dalian, Liaoning, 116027, China. Huirong_jing@163.com.
authors.forename: Huirong
authors.initials: H
authors.lastname: Jing
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Dalian Medical University, No. 467 Zhongshan Road, Dalian, Liaoning, 116027, China. Hui_zhang2014@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D000097905:Roundabout Proteins
chemical_list: D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C578248:MIRN219 microRNA, human
keywords: Glioblastoma; Invasion; Proliferation; ROBO1; miR-219-5p