title: Regulation of the Rev1-pol ζ complex during bypass of a DNA interstrand cross-link.
abstract: DNA interstrand cross-links (ICLs) are repaired in S phase by a complex, multistep mechanism involving translesion DNA polymerases. After replication forks collide with an ICL, the leading strand approaches to within one nucleotide of the ICL ("approach"), a nucleotide is inserted across from the unhooked lesion ("insertion"), and the leading strand is extended beyond the lesion ("extension"). How DNA polymerases bypass the ICL is incompletely understood. Here, we use repair of a site-specific ICL in Xenopus egg extracts to study the mechanism of lesion bypass. Deep sequencing of ICL repair products showed that the approach and extension steps are largely error-free. However, a short mutagenic tract is introduced in the vicinity of the lesion, with a maximum mutation frequency of ~1%. Our data further suggest that approach is performed by a replicative polymerase, while extension involves a complex of Rev1 and DNA polymerase ζ. Rev1-pol ζ recruitment requires the Fanconi anemia core complex but not FancI-FancD2. Our results begin to illuminate how lesion bypass is integrated with chromosomal DNA replication to limit ICL repair-associated mutagenesis.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
authors.forename: Magda
authors.initials: M
authors.lastname: Budzowska
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
authors.forename: Thomas G W
authors.initials: TG
authors.lastname: Graham
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Sobeck
authors.affiliation: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Shou
authors.initials: S
authors.lastname: Waga
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA Howard Hughes Medical Institute, Boston, MA, USA johannes_walter@hms.harvard.edu.
authors.forename: Johannes C
authors.initials: JC
authors.lastname: Walter
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D004260:DNA Repair; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D051856:Fanconi Anemia Complementation Group Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D046912:Multiprotein Complexes; D016296:Mutagenesis; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D054875:Ubiquitination; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D000099270:Y-Family DNA Polymerases
chemical_list: D004259:DNA-Directed DNA Polymerase; D051856:Fanconi Anemia Complementation Group Proteins; D046912:Multiprotein Complexes; D009713:Nucleotidyltransferases; D018809:Proliferating Cell Nuclear Antigen; D029867:Xenopus Proteins; C576817:FANCD2 protein, Xenopus; C546473:FANCI Protein, Xenopus; C425929:DNA polymerase zeta; C000601751:Rev1 protein, Xenopus; D000099270:Y-Family DNA Polymerases
keywords: Fanconi anemia; genome stability; interstrand cross‐link repair; translesion synthesis