title: HPLC-NMR and HPLC-MS investigation of antimicrobial constituents in Cystophora monilifera and Cystophora subfarcinata.
abstract: The crude dichloromethane extracts of the marine brown algae Cystophora monilifera and Cystophora subfarcinata were subjected to phytochemical profiling. This enabled the structures of both new and known phloroglucinols to be dereplicated and proposed using HPLC-NMR and HPLC-MS. Subsequent isolation confirmed the presence of four new and eight previously reported compounds. Five of the isolated phloroglucinols displayed selective antimicrobial activity.
authors.affiliation: School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi), RMIT University, GPO Box 2476V, Melbourne, Victoria 3001, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Brkljača
authors.affiliation: School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi), RMIT University, GPO Box 2476V, Melbourne, Victoria 3001, Australia. Electronic address: sylvia.urban@rmit.edu.au.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Urban
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008752:Methylene Chloride / Q000737:chemistry; D015394:Molecular Structure; D000459:Phaeophyceae / Q000737:chemistry*; D010696:Phloroglucinol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D008752:Methylene Chloride; D010696:Phloroglucinol
keywords: Antimicrobial activity; Brown alga; Cystophora; Dereplication; HPLC–MS; HPLC–NMR; Phloroglucinol