title: Impact of Vesicular Stomatitis Virus M Proteins on Different Cellular Functions.
abstract: Three different matrix (M) proteins termed M1, M2 and M3 have been described in cells infected with vesicular stomatitis virus (VSV). Individual expression of VSV M proteins induces an evident cytopathic effect including cell rounding and detachment, in addition to a partial inhibition of cellular protein synthesis, likely mediated by an indirect mechanism. Analogous to viroporins, M1 promotes the budding of new virus particles; however, this process does not produce an increase in plasma membrane permeability. In contrast to M1, M2 and M3 neither interact with the cellular membrane nor promote the budding of double membrane vesicles at the cell surface. Nonetheless, all three species of M protein interfere with the transport of cellular mRNAs from the nucleus to the cytoplasm and also modulate the redistribution of the splicing factor. The present findings indicate that all three VSV M proteins share some activities that interfere with host cell functions.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Redondo
authors.affiliation: Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Madan
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Alvarez
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Carrasco
mesh_terms: D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D002474:Cell-Free System; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000502:physiology; D046529:Microscopy, Electron, Transmission; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000502:physiology*; D057074:Virus Release
chemical_list: D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H; C094460:M protein, Vesicular stomatitis virus; D012333:RNA, Messenger; D012367:RNA, Viral; D014763:Viral Matrix Proteins