title: Altered Expression of NF- κ B and SP1 after Exposure to Advanced Glycation End-Products and Effects of Neurotrophic Factors in AGEs Exposed Rat Retinas.
abstract: To determine the effect of advanced glycation end-products (AGEs) on neurite regeneration, and also to determine the regenerative effects of different neurotrophic factors (NTFs) on rat retinal explants, the retinas of SD rats were cultured in three-dimensional collagen gels and incubated in 6 types of media: (1) serum-free control culture media; (2) 100 μg/mL AGEs-BSA media; (3) AGEs-BSA + 100 ng/mL neurotrophin-4 (NT-4) media; (4) AGEs-BSA + 100 ng/mL hepatocyte growth factor media; (5) AGEs-BSA + 100 ng/mL glial cell line-derived neurotrophic factor media; or (6) AGEs-BSA + 100 µM tauroursodeoxycholic acid media. After 7 days, the number of regenerating neurites was counted. The explants were immunostained for nuclear factor-κB (NF-κB) and specificity protein 1 (SP1). Statistical analyses were performed by one-way ANOVA. In retinas incubated with AGEs, the numbers of neurites were fewer than in control. All of the NTFs increased the number of neurites, and the increase was more significant in the NT-4 group. The number of NF-κB and SP1 immunopositive cells was higher in retinas exposed to AGEs than in control. All of the NTFs decreased the number of NF-κB immunopositive cells but did not significantly affect SP1 expression. These results demonstrate the potential of the NTFs as axoprotectants in AGEs exposed retinal neurons.
authors.affiliation: Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, Chiba Prefecture 260-8670, Japan.
authors.forename: Guzel
authors.identifier: 0000-0002-7715-7466
authors.initials: G
authors.lastname: Bikbova
authors.affiliation: Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, Chiba Prefecture 260-8670, Japan.
authors.forename: Toshiyuki
authors.identifier: 0000-0001-6127-0707
authors.initials: T
authors.lastname: Oshitari
authors.affiliation: Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, Chiba Prefecture 260-8670, Japan.
authors.forename: Takayuki
authors.identifier: 0000-0002-8178-8391
authors.initials: T
authors.lastname: Baba
authors.affiliation: Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, Chiba Prefecture 260-8670, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D051381:Rats; D012160:Retina / Q000187:drug effects*; D016329:Sp1 Transcription Factor / Q000187:drug effects* / Q000378:metabolism; D013655:Taurochenodeoxycholic Acid / Q000494:pharmacology
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D017127:Glycation End Products, Advanced; D016328:NF-kappa B; D009414:Nerve Growth Factors; D016329:Sp1 Transcription Factor; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine; D017228:Hepatocyte Growth Factor; C073922:neurotrophin 4