title: Yif1B Is Involved in the Anterograde Traffic Pathway and the Golgi Architecture.
abstract: Yif1B is an intracellular membrane-bound protein belonging to the Yip family, shown previously to control serotonin 5-HT1A receptor targeting to dendrites. Because some Yip proteins are involved in the intracellular traffic between the ER and the Golgi, here we investigated the precise localization of Yif1B in HeLa cells. We found that Yif1B is not resident into the Golgi, but rather belongs to the IC compartment. After analyzing the role of Yif1B in protein transport, we showed that the traffic of the VSVG protein marker was accelerated in Yif1B depleted HeLa cells, as well as in hippocampal neurons from Yif1B KO mice. Conversely, Yif1B depletion in HeLa cells did not change the retrograde traffic of ShTx. Interestingly, in long term depletion of Yif1B as in Yif1B KO mice, we observed a disorganized Golgi architecture in CA1 pyramidal hippocampal neurons, which was confirmed by electron microscopy. However, because short term depletion of Yif1B did not change Golgi structure, it is likely that the implication of Yif1B in anterograde traffic does not rely on its role in structural organization of the Golgi apparatus, but rather on its shuttling between the ER, the IC and the Golgi compartments.
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Jeanine
authors.initials: J
authors.lastname: Alterio
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Masson
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Jorge
authors.initials: J
authors.lastname: Diaz
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Chachlaki
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Haysam
authors.initials: H
authors.lastname: Salman
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Areias
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Sana
authors.initials: S
authors.lastname: Al Awabdh
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Michel Boris
authors.initials: MB
authors.lastname: Emerit
authors.affiliation: INSERM U894, Centre de Psychiatrie et Neurosciences, Paris, F-75014, France.|Université Paris Descartes, Sorbonne Paris Cité - Paris 5, UMR U894, Paris, F-75014, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Darmon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D021381:Protein Transport; D051381:Rats; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D033921:Vesicular Transport Proteins
keywords: Golgi fragmentation; Yif1B; Yip family; anterograde transport; intermediate compartment