title: Spatiotemporal expression of Rhomboid domain containing 2 (Rhbdd2) during rat development.
abstract: Over the last few years rhomboid genes have gained interest because of its association with cancer and neurodegenerative diseases. In previous studies, we demonstrated that human RHBDD2 is over-expressed in the advanced stages of breast and colorectal cancers, suggesting a favorable role in cell proliferation. So far little is known about the expression of RHBDD2 in other tissues and other species, and because of similarities between cancer and embryonic cells, this study focused on the evaluation of Rhbdd2 expression in embryonic and adult rat tissues. By IHC and RT-PCR, Rhbdd2 was identified in early stages of most tissues analyzed, with high expression in brain, spinal cord, kidney and embryonic skin. In adult tissues, the expression remained elevated while salivary glands became positive. Furthermore, Rhbdd2 showed a high expression in the most proliferative stages of the rat mammary gland. Indeed, similar findings were observed in the mouse mammary epithelial cell line HC11, in which Rhbdd2 resides in the Golgi apparatus, and at different stages of mouse mammary gland development. Therefore, Rhbdd2 would be implicated in embryonic and adult tissue proliferation.
authors.affiliation: CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: V A
authors.initials: VA
authors.lastname: Ferretti
authors.affiliation: CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Canzoneri
authors.affiliation: Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.affiliation: CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: M V
authors.initials: MV
authors.lastname: Croce
authors.affiliation: CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Abba
authors.affiliation: CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina. Electronic address: ezequiellacunza@hotmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Lacunza
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism / Q000503:physiopathology; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000503:physiopathology; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics; D011247:Pregnancy; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008565:Membrane Proteins; D009363:Neoplasm Proteins
keywords: Development; Proliferation; Rat; Rhbdd2