title: Recent progress in luminescent proteins development.
abstract: Bioimaging requires not only high sensitivity but also minimal invasiveness. Bioimaging using luminescent proteins is potentially free from problems such as photo-induced damage or an undesirable physical reaction to the sample, which are often caused by illumination with an external light required in fluorescence imaging. The recent development of several luminescent proteins and substrates have greatly improved the brightness of luminescence imaging, facilitating its application by many researchers. In this short review, we summarize recent advances in development of luminescent proteins, substrates, and indicators.
authors.affiliation: The Center for Brain Integration Research, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Saito
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, 8-1, Mihogaoka, Ibaraki, Osaka 567-0047, Japan. Electronic address: ng1@sanken.osaka-u.ac.jp.
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nagai
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055808:Drug Discovery; D005456:Fluorescent Dyes / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D057054:Molecular Imaging / Q000379:methods*; D011485:Protein Binding
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins