title: Vertical Distribution of Bacterial Community Diversity and Water Quality during the Reservoir Thermal Stratification.
abstract: Reservoir thermal stratification drives the water temperature and dissolved oxygen gradient, however, the characteristic of vertical water microbial community during thermal stratification is so far poorly understood. In this work, water bacterial community diversity was determined using the Illumina Miseq sequencing technique. The results showed that epilimnion, metalimnion and hypolimnion were formed steadily in the JINPEN drinking water reservoir. Water temperature decreased steadily from the surface (23.11 °C) to the bottom (9.17 °C). Total nitrogen ranged from 1.07 to 2.06 mg/L and nitrate nitrogen ranged from 0.8 to 1.84 mg/L. The dissolved oxygen concentration decreased sharply below 50 m, and reached zero at 65 m. The Miseq sequencing revealed a total of 4127 operational taxonomic units (OTUs) with 97% similarity, which were affiliated with 15 phyla including Acidobacteria, Actinobacteria, Armatimonadetes, Bacteroidetes, Caldiserica, Chlamydiae, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The highest Shannon diversity was 4.41 in 45 m, and the highest Chao 1 diversity was 506 in 5 m. Rhodobacter dominated in 55 m (23.24%) and 65 m (12.58%). Prosthecobacter dominated from 0.5 to 50 m. The heat map profile and redundancy analysis (RDA) indicated significant difference in vertical water bacterial community composition in the reservoir. Meanwhile, water quality properties including dissolved oxygen, conductivity, nitrate nitrogen and total nitrogen have a dramatic influence on vertical distribution of bacterial communities.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China. zhanghaihan@xauat.edu.cn.
authors.forename: Hai-Han
authors.initials: HH
authors.lastname: Zhang
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China. chenshengnan@xauat.edu.cn.
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Chen
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China. huangtinglin@xauat.edu.cn.
authors.forename: Ting-Lin
authors.initials: TL
authors.lastname: Huang
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China. hs_weixing@163.com.
authors.forename: Wei-Xing
authors.initials: WX
authors.lastname: Ma
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China. xujinlan@xauat.edu.cn.
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Xu
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China. sunxin@xauat.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D009584:Nitrogen / Q000032:analysis; D013696:Temperature; D014871:Water Microbiology; D060753:Water Quality
chemical_list: D009584:Nitrogen
keywords: high-throughput sequencing; reservoir; thermal stratification; water bacterial community