title: A novel micro-emulsion and micelle assembling method to prepare DEC205 monoclonal antibody coupled cationic nanoliposomes for simulating exosomes to target dendritic cells.
abstract: Cationic biomimetic exosomes were prepared using a novel micro-emulsion and micelle assembling method by introducing DEC205 monoclonal antibody as specific ligand to target dendritic cells (DCs). The Box-Behnken experimental design was applied for optimization of nanoliposomes (NLip) and DEC205 monoclonal antibody was then conjugated on the surface of NLip (DEC205-NLip). NLip and DEC205-NLip respectively had an average size of 62.7 ± 6.33 nm and 81.64 ± 4.25 nm, zeta potential of +30.5 ± 2.3 mV and +19.8 ± 1.8 mV and encapsulation efficiency of 91.02 ± 3.1% and 93.10 ± 2.2%. In addition, the toxicity studies confirmed DEC205 monoclonal antibody could significantly reduce the cytotoxicity of the cationic lipid against DCs. And the cellular uptake experiment evaluated the significant targeting effect of the DEC205 monoclonal antibody on DC cells. In conclusion, the novel method presented here to prepare biomimetic exosomes was an efficient approach to develop antigen carriers for specific DCs targeting.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, P.O. Box 42, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, P.O. Box 42, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Chang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, P.O. Box 42, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Zhongyan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, P.O. Box 42, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, P.O. Box 42, Shenyang, Liaoning Province 110016, PR China. Electronic address: kexinbest1128@163.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
mesh_terms: D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry*; D002412:Cations / Q000008:administration & dosage / Q000737:chemistry*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects*; D004655:Emulsions / Q000008:administration & dosage / Q000737:chemistry*; D055354:Exosomes / Q000187:drug effects*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry*; D008823:Micelles; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size
chemical_list: D000911:Antibodies, Monoclonal; D002412:Cations; D004655:Emulsions; D008055:Lipids; D008081:Liposomes; D008823:Micelles
keywords: DEC205 monoclonal antibody; Dendritic cells; Exosomes; Micelle; Micro-emulsion; Nanoliposomes