title: Identification and purification of target protein using affinity resin bearing a photo-labile linker.
abstract: This Letter presents an effective method for the identification of target proteins of bioactive compounds such as drugs, natural products, and intrinsic ligands, using an affinity resin. The application of a photo-labile linker to an affinity resin enabled the selective elution of a target protein by irradiation for a short duration at 4 °C while leaving a large amount of non-specific binding protein on the resin. We have named this method the 'STEAP' method (selective target elution from affinity resins with photo-labile linker). Only a target protein that can bind the bioactive compound, the so-called 'active' protein, is eluted by the selective cleavage of the linker between the solid matrix and the target compound, and therefore, it is worth considering the potential of this method for the hyper-purification of proteins.
authors.affiliation: Advanced Medical Research Center, Hyogo University of Health Science, 1-3-6 Minatojima, Kobe 650-8530, Japan; KOBE Chemical Genetics., Inc., 2-2-2 Minatojima-nakamachi, Chuo-ku, Kobe 650-8530, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Mabuchi
authors.affiliation: Advanced Medical Research Center, Hyogo University of Health Science, 1-3-6 Minatojima, Kobe 650-8530, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Discovery Research Department, Chugai Pharmaceutical Co., Ltd, 200 Kajiwara, Kamakura 247-8530, Kanagawa, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Haramura
authors.affiliation: Advanced Medical Research Center, Hyogo University of Health Science, 1-3-6 Minatojima, Kobe 650-8530, Japan; KOBE Chemical Genetics., Inc., 2-2-2 Minatojima-nakamachi, Chuo-ku, Kobe 650-8530, Japan. Electronic address: tanaka-a@huhs.ac.jp.
authors.forename: Akito
authors.initials: A
authors.lastname: Tanaka
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002846:Chromatography, Affinity; D016503:Drug Delivery Systems; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D011506:Proteins; D012117:Resins, Synthetic
keywords: Affinity resin; Antibodies; Photochemistry; Protein purification; Target identification