title: Antiproliferative activity of flavonoids from Croton sphaerogynus Baill. (Euphorbiaceae).
abstract: Croton sphaerogynus is a shrub from the Atlantic Rain Forest in southeastern Brazil. A lyophilized crude EtOH extract from leaves of C. sphaerogynus, obtained by maceration at room temperature (seven days), was suspended in methanol and partitioned with hexane. The purified MeOH phase was fractionated over Sephadex LH-20 yielding five fractions (F1-F5) containing flavonoids, as characterized by HPLC-DAD and HPLC-MS analyses. The antiproliferative activity of the crude EtOH extract, MeOH and hexane phases, and fractions F1-F5 was evaluated on in vitro cell lines NCI-H460 (nonsmall cell lung), MCF-7 (breast cancer), and U251 (glioma). The MeOH phase showed activity (mean log GI50 0.54) higher than the hexane phase and EtOH extract (mean log GI50 1.13 and 1.19, resp.). F1 exhibited activity against NCI-H460 (nonsmall cell lung) (GI50 1.2 μg/mL), which could be accounted for the presence of flavonoids and/or diterpenes. F4 showed moderate activity (mean log GI50 1.05), while F5 showed weak activity (mean log GI50 1.36). It is suggested that the antiproliferative activity of the crude EtOH extract and MeOH phase is accounted for a synergistic combination of flavonoids and diterpenes.
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Kátia Pereira
authors.initials: KP
authors.lastname: dos Santos
authors.affiliation: Universidade Paulista, Rua Apeninos 267, 01533-000 São Paulo, SP, Brazil.
authors.forename: Lucimar B
authors.initials: LB
authors.lastname: Motta
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Deborah Y A C
authors.initials: DY
authors.lastname: Santos
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Maria L F
authors.initials: ML
authors.lastname: Salatino
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Salatino
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Marcelo J Pena
authors.initials: MJ
authors.lastname: Ferreira
authors.affiliation: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua Professor Arthur Riedel 275, 09972-270 Diadema, SP, Brazil.
authors.forename: João Henrique G
authors.initials: JH
authors.lastname: Lago
authors.affiliation: Divisão de Farmacologia e Toxicologia, CPQBA, CP 6171, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil.
authors.forename: Ana Lúcia T G
authors.initials: AL
authors.lastname: Ruiz
authors.affiliation: Divisão de Farmacologia e Toxicologia, CPQBA, CP 6171, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil.
authors.forename: João E
authors.initials: JE
authors.lastname: de Carvalho
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Furlan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D029802:Croton / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D018515:Plant Leaves / Q000737:chemistry; D013053:Spectrophotometry; D053719:Tandem Mass Spectrometry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids