title: Development and characterization of microsatellite markers for Veratrum maackii (Melanthiaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in Veratrum maackii (Melanthiaceae) to assess the pattern of population genetic structures across the species' distribution.
  
  
  RESULTS
  Ten polymorphic loci were isolated from V. maackii using 454 shotgun pyrosequencing and the biotin capture method. The number of alleles per locus ranged from three to 19. The observed heterozygosity and expected heterozygosity ranged from 0.143 to 0.929 and 0.269 to 0.904, respectively.
  
  
  CONCLUSIONS
  These newly developed microsatellite markers are useful for assessing the genetic diversity, population structure, and demographic history of V. maackii across its distribution range.
authors.affiliation: Botanical Gardens, Tohoku University, Aoba, Sendai 980-0862, Japan.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Ecology and Evolutionary Biology, Graduate School of Sciences, Tohoku University, Aoba, Sendai 980-8578, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iijima
authors.affiliation: Botanical Gardens, Tohoku University, Aoba, Sendai 980-0862, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Maki
keywords: 454 shotgun sequencing; Melanthiaceae; Veratrum maackii; genetic structure; microsatellite; multiplex PCR