title: Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium.
abstract: Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct regions of the target DNA sequence, in order to amplify target DNA in a highly specific and rapid manner. A LAMP assay was designed to the pdhD gene of M. genitalium, and the limit of detection of the assay was determined as 10 fg of M. genitalium genomic DNA, equating to ~16 copies of the M. genitalium genome, which was equally sensitive as a gold standard 16S rRNA polymerase chain reaction assay.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
authors.forename: Thomas
authors.initials: T
authors.lastname: Edwards
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
authors.forename: Patricia
authors.initials: P
authors.lastname: Burke
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
authors.forename: Helen B
authors.initials: HB
authors.lastname: Smalley
authors.affiliation: Mast Group Ltd., Bootle, Liverpool, United Kingdom.
authors.forename: Liz
authors.initials: L
authors.lastname: Gillies
authors.affiliation: Public Health England - Bristol Laboratory, Specialist Virology Centre, Myrtle Road, Bristol, United Kingdom.
authors.forename: Denise
authors.initials: D
authors.lastname: Longhurst
authors.affiliation: Public Health England - Bristol Laboratory, Specialist Virology Centre, Myrtle Road, Bristol, United Kingdom.
authors.forename: Barry
authors.initials: B
authors.lastname: Vipond
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom. Electronic address: G.Hobbs@ljmu.ac.uk.
authors.forename: Glyn
authors.initials: G
authors.lastname: Hobbs
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D045704:Mycoplasma genitalium / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers
keywords: Diagnostic; Loop mediated isothermal amplification; Mycoplasma genitalium; PCR; Rapid testing; Urethritis