title: Detection of cerebrospinal fluid leakage by specific measurement of transferrin glycoforms.
abstract: A simple and rapid detection of cerebrospinal fluid (CSF) leakage would benefit spine surgeons making critical postoperative decisions on patient care. We have assessed novel approaches to selectively determine CSF β2-transferrin (β2TF), an asialo-transferrin (aTF) biomarker, without interference from serum sialo-transferrin (sTF) in test samples. First, we performed mild periodate oxidation to selectively generate aldehyde groups in sTF for capture with magnetic hydrazide microparticles, and selective removal with a magnetic separator. Using this protocol sTF was selectively removed from mixtures of CSF and serum containing CSF aTF (β2TF) and serum sTF, respectively. Second, a two-step enzymatic method was developed with neuraminidase and galactose oxidase for generating aldehyde groups in sTF present in CSF and serum mixtures for magnetic hydrazide microparticle capture. After selectively removing sTF from mixtures of CSF and serum, ELISA could detect significant TF signal only in CSF, while the TF signal in serum was negligible. The new approach for selective removal of only sTF in test samples will be promising for the required intervention by a spine surgeon.
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA.
authors.forename: Seok-Joon
authors.initials: SJ
authors.lastname: Kwon
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA.
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA.|Department of Biology, Rensselaer Polytechnic Institute, Troy, NY, USA.|Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA.
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
authors.affiliation: Neurological Surgery, P.C., Rockville Centre, NY, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Sonstein
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA.|Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA.|Neurological Surgery, P.C., Rockville Centre, NY, USA.|Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D001212:Asialoglycoproteins / Q000097:blood / Q000134:cerebrospinal fluid / Q000737:chemistry; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D065634:Cerebrospinal Fluid Leak / Q000175:diagnosis*; D006801:Humans; D012795:Sialoglycoproteins / Q000097:blood / Q000134:cerebrospinal fluid / Q000737:chemistry; D014168:Transferrin / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid / Q000737:chemistry
chemical_list: D001212:Asialoglycoproteins; D015415:Biomarkers; D012795:Sialoglycoproteins; D014168:Transferrin; C017031:asialotransferrins; C519074:sialotransferrin, human
keywords: Cerebrospinal fluid; Glycoforms; Transferrin