title: Oxidant/antioxidant status, paraoxonase activity, and lipid profile in plasma of ovariectomized rats under the influence of estrogen, estrogen combined with progesterone, and genistein.
abstract: BACKGROUND
  The aim of this study was to investigate whether estradiol (E2), E2 combined with progesterone (Prog) (E2/Prog), and genistein (Gen) treatment had antioxidative and anti-hyperlipidemic effects in the plasma of ovariectomized (OVX) rats.
  
  
  METHODS
  Adult female Sprague-Dawley rats were divided into five groups. Rats in all groups, except for those in a sham group, underwent bilateral ovariectomy under general anesthesia. The groups were as follows: sham group; control OVX group; group treated with estrogen (0.014 mg/kg 17-β E2); group treated with a combination of E2 and Prog (0.014 mg/kg 17-β E2 plus 0.028 mg/kg drospirenone), and group treated with Gen (10 mg/kg/day). Plasma of rats of each treatment group was analyzed to determine the total antioxidant status, total oxidant status, paraoxonase activity, lipid profile, high-density lipoprotein (HDL-chol), low-density lipoprotein (LDL-chol), total cholesterol (Total-C), triacylglycerols, lipoprotein (a), and oxidative stress index.
  
  
  RESULTS
  Plasma Total-C levels and body weight increased in all the OVX groups compared with the sham group (P<0.005). The group treated with E2 had significantly elevated total oxidant status, oxidative stress index, LDL-chol, and Total-C compared with the control group (P<0.005). Gen treatment might lead to lower LDL-chol and Total-C levels compared with E2 treatment.
  
  
  CONCLUSIONS
  Gen treatment might be preferred to E2 treatment for treatment of menopausal symptoms in patients at risk for cardiovascular diseases. However, considering the small sample size of this study, larger studies are needed in this area.
authors.affiliation: Department of Obstetrics and Gynecology, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Agacayak
authors.affiliation: Department of Obstetrics and Gynecology, Idil State Hospital, Sirnak, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Basaranoglu
authors.affiliation: Department of Obstetrics and Gynecology, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Senem Yaman
authors.initials: SY
authors.lastname: Tunc
authors.affiliation: Department of Obstetrics and Gynecology, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Mehmet Sait
authors.initials: MS
authors.lastname: Icen
authors.affiliation: Department of Obstetrics and Gynecology, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Fatih Mehmet
authors.initials: FM
authors.lastname: Findik
authors.affiliation: Department of Biochemistry, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Kaplan
authors.affiliation: Department of Biochemistry, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Evliyaoglu
authors.affiliation: Department of Obstetrics and Gynecology, Dicle University School of Medicine, Diyarbakir, Turkey.
authors.forename: Talip
authors.initials: T
authors.lastname: Gul
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D001835:Body Weight; D004958:Estradiol / Q000494:pharmacology; D015914:Estrogen Replacement Therapy; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D008055:Lipids / Q000097:blood*; D010052:Ovariectomy; D016877:Oxidants / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D011372:Progestins / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D004967:Estrogens; D008055:Lipids; D016877:Oxidants; D011372:Progestins; D011374:Progesterone; D004958:Estradiol; D019833:Genistein; D043303:Aryldialkylphosphatase
keywords: cardiovascular diseases; genistein; menopause