title: The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails: A comparative study.
abstract: Biomass enzymatic hydrolysis depends on the pretreatment methods employed, the composition of initial feedstock and the enzyme cocktail used to release sugars for subsequent fermentation into ethanol. In this study, sugarcane bagasse was pretreated with 1% H2SO4 and 1% NaOH and the biomass saccharification was performed with 8% solids loading using 10 FPase units/g of bagasse of the enzymatic extract from Chrysoporthe cubensis and three commercial cocktails for a comparative study. Overall, the best glucose and xylose release was obtained from alkaline pretreated sugarcane bagasse. The C. cubensis extract promoted higher release of glucose (5.32 g/L) and xylose (9.00 g/L) than the commercial mixtures. Moreover, the C. cubensis extract presented high specific enzyme activities when compared to commercial cocktails mainly concerning to endoglucanase (331.84 U/mg of protein), β-glucosidase (29.48 U/mg of protein), β-xylosidase (2.95 U/mg of protein), pectinase (127.46 U/mg of protein) and laccase (2.49 U/mg of protein).
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil; CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Gabriela Piccolo
authors.initials: GP
authors.lastname: Maitan-Alfenas
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
authors.forename: Evan Michael
authors.initials: EM
authors.lastname: Visser
authors.affiliation: Department of Forest Engineering, Universidade Federal de Mato Grosso, Av. Alexandre Ferronato, 1200, 78557-267 Sinop, MT, Brazil.
authors.forename: Rafael Ferreira
authors.initials: RF
authors.lastname: Alfenas
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
authors.forename: Bráulio Ris G
authors.initials: BR
authors.lastname: Nogueira
authors.affiliation: Department of Biotechnology, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, SP, Brazil.
authors.forename: Guilherme Galvão
authors.initials: GG
authors.lastname: de Campos
authors.affiliation: Department of Biotechnology, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, SP, Brazil.
authors.forename: Adriane Ferreira
authors.initials: AF
authors.lastname: Milagres
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil. Electronic address: vmonteze@ufv.br.
authors.forename: Valéria Monteze
authors.initials: VM
authors.lastname: Guimarães
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002241:Carbohydrates / Q000096:biosynthesis* / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004789:Enzyme Activation; D006867:Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D031786:Saccharum / Q000737:chemistry* / Q000382:microbiology; D013213:Starch
chemical_list: D002241:Carbohydrates; D002482:Cellulose; D013213:Starch; C027433:bagasse; D006867:Hydrolases
keywords: Biomass pretreatment; Chrysoporthe cubensis; Enzyme mixtures; Saccharification