title: Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect.
abstract: Homocysteine thiolactone was demonstrated to inhibit the growth of 10-12-day-old chicken embryo cardiac tissue explants at 7 × 10⁻⁹ -1 × 10⁻³ M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10⁻³ M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10⁻³ M) and ouabain at concentrations regulating the signal-transducing (1 × 10⁻¹⁰ M) and pumping (1 × 10⁻⁸ M) functions of Na⁺,K⁺ -ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na⁺,K⁺ -ATPase pumping function.
authors.affiliation: Pavlov Institute of Physiology Russian Academy of Sciences , St. Petersburg , Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Lopatina
authors.forename: A V
authors.initials: AV
authors.lastname: Kipenko
authors.forename: V A
authors.initials: VA
authors.lastname: Penniyaynen
authors.forename: N A
authors.initials: NA
authors.lastname: Pasatetskaya
authors.forename: D
authors.initials: D
authors.lastname: Djuric
authors.forename: B V
authors.initials: BV
authors.lastname: Krylov
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D006321:Heart / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010042:Ouabain / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D004791:Enzyme Inhibitors; D006710:Homocysteine; D010042:Ouabain; C007957:homocysteine thiolactone; D000254:Sodium-Potassium-Exchanging ATPase
keywords: K+-ATPase; Na+; PlasDIC-contrast; cardiac tissue explants; homocysteine thiolactone; organotypic culture