title: [Genotype study on hepatitis C among entry foreigners in China].
abstract: OBJECTIVE
  To monitor the the genotype and subgenotype c of hepatitis C among the immigrations. To identify the new genotype and trace the transmission.
  
  
  METHODS
  From 2009 to 2013, 235 positive Anti-HCV samples were collected from Sichuan, Liaoning, Guangdong, Shenzhen and Yunnan ITHC. Specific PCR primers were used to amplify the HCV Core, then the PCR products were sequenced by genetic analyzer. The genotypes were identified by aligning the GenBank reference sequences and constructing the phylogenetic tree of Core.
  
  
  RESULTS
  One hundred and twenty-nine samples showed HCV-RNA positive (54.9%) in 235 samples which were anti-HCV positive. We detected six kinds of genotypes in 115 cases of sequncing successfully, of which 72 cases of genotype 1 (G1, 62.6%), followed by G3 (18.2%), G2 (9.6%), G4 (6%), G6 (2.6%) and G5 (0.9%). Genotype 1b was the most common subtype, accounting for 47% of all infections. The phylogenetic tress indicated the HCV 4 strains in immigrations had high affinity with that in Egypt and Europe, while HCV 6 strains closed with that in China.
  
  
  CONCLUSIONS
  HCV 1b is the advantage of popular genotype in HCV carriers. Subtype 4 may be a possible new genotype transmited into China. The immigrations may be the sources of new genotype of HCV.
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China.
authors.forename: Lübo
authors.initials: L
authors.lastname: Tian
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China.
authors.forename: Liming
authors.initials: L
authors.lastname: He
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China; Email: h121260@126.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Fan
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China.
authors.forename: Guolong
authors.initials: G
authors.lastname: Gao
authors.affiliation: Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D002681:China; D054242:Emigrants and Immigrants; D005838:Genotype; D016174:Hepacivirus; D006526:Hepatitis C; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction