title: IMART software for correction of motion artifacts in images collected in intravital microscopy.
abstract: Intravital microscopy is a uniquely powerful tool, providing the ability to characterize cell and organ physiology in the natural context of the intact, living animal. With the recent development of high-resolution microscopy techniques such as confocal and multiphoton microscopy, intravital microscopy can now characterize structures at subcellular resolution and capture events at sub-second temporal resolution. However, realizing the potential for high resolution requires remarkable stability in the tissue. Whereas the rigid structure of the skull facilitates high-resolution imaging of the brain, organs of the viscera are free to move with respiration and heartbeat, requiring additional apparatus for immobilization. In our experience, these methods are variably effective, so that many studies are compromised by residual motion artifacts. Here we demonstrate the use of IMART, a software tool for removing motion artifacts from intravital microscopy images collected in time series or in three dimensions.
authors.affiliation: Division of Nephrology; School of Medicine; Indiana University; Indianapolis, IN USA.
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Dunn
authors.affiliation: Video and Image Processing Laboratory; School of Electrical and Computer Engineering; Purdue University; West Lafayette, IN USA.
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Lorenz
authors.affiliation: Department of Electrical and Computer Engineering; Indiana University; Indianapolis, IN USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Salama
authors.affiliation: Video and Image Processing Laboratory; School of Electrical and Computer Engineering; Purdue University; West Lafayette, IN USA.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Delp
keywords: fluorescence microscopy; image analysis; image processing; image registration; in vivo imaging; intravital microscopy; motion artifact; multiphoton microscopy