title: Orally Administered Chitosan-Coated Polycaprolactone Nanoparticles Containing Curcumin Attenuate Metastatic Melanoma in the Lungs.
abstract: The study was aimed to evaluate the effect of orally administered chitosan-coated nanoparticles containing curcumin on metastatic melanoma. Chitosan-coated nanoparticles containing curcumin were prepared, and their antimetastatic activity was investigated both in vitro and in vivo. Curcumin decreased cell viability and induced apoptosis of B16F10 melanoma cells. We observed that curcumin significantly decreased the expression of metalloproteinases, which are known to be associated with migration and proliferation of cancer cells. Importantly, treatment with chitosan-coated nanoparticles containing curcumin decreased pulmonary tumor formation in a murine model of experimental metastasis. Histological analyses confirmed the macroscopic results in which lungs of mice treated with curcumin-loaded chitosan-coated polycaprolactone nanoparticles had only a few small nodules and most of them were free of melanoma. Our findings indicate that nanoparticles coated with the mucoadhesive polymer chitosan containing curcumin may be a promising approach and/or intervention for the treatment of malignant melanoma.
authors.affiliation: Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Gecioni
authors.initials: G
authors.lastname: Loch-Neckel
authors.affiliation: Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Lorena
authors.initials: L
authors.lastname: Santos-Bubniak
authors.affiliation: Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Letícia
authors.initials: L
authors.lastname: Mazzarino
authors.affiliation: Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Amanda V
authors.initials: AV
authors.lastname: Jacques
authors.affiliation: Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Moccelin
authors.affiliation: Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Maria Claúdia
authors.initials: MC
authors.lastname: Santos-Silva
authors.affiliation: Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900, Brazil.
authors.forename: Elenara
authors.initials: E
authors.lastname: Lemos-Senna
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D048271:Chitosan; D003474:Curcumin / Q000008:administration & dosage* / Q000627:therapeutic use*; D005260:Female; D007408:Intestinal Absorption; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D009362:Neoplasm Metastasis; D011091:Polyesters; D012878:Skin Neoplasms; D000096142:Cutaneous Malignant Melanoma
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D003474:Curcumin
keywords: B16F10 cells; chitosan; curcumin; drug delivery systems; in vitro models; intestinal absorption; metastatic melanoma; mouse model; muco adhesive; nanotechnology