title: A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
abstract: A sensitive and noble amperometric horseradish peroxidase (HRP) biosensor is fabricated via the deposition of gold nanoparticles (AuNPs) onto a three-dimensional (3D) porous carbonized chicken eggshell membrane (CESM). Due to the synergistic effects of the unique porous carbon architecture and well-distributed AuNPs, the enzyme-modified electrode shows an excellent electrochemical redox behavior. Compared with bare glass carbon electrode (GCE), the cathodic peak current of the enzymatic electrode increases 12.6 times at a formal potential of -100 mV (vs. SCE) and charge-transfer resistance decreases 62.8%. Additionally, the AuNPs-CESM electrode exhibits a good biocompatibility, which effectively retains its bioactivity with a surface coverage of HRP 6.39×10(-9) mol cm(-2) (752 times higher than the theoretical monolayer coverage of HRP). Furthermore, the HRP-AuNPs-CESM-GCE electrode, as a biosensor for H2O2 detection, has a good accuracy and high sensitivity with the linear range of 0.01-2.7 mM H2O2 and the detection limit of 3 μM H2O2 (S/N = 3).
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Science and Chemical Engineering, Harbin Engineering University, Harbin, China.
authors.forename: Zhuangjun
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Li
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Du
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Chenming
authors.initials: C
authors.lastname: Liu
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Environmental Protection Research Institute of Light Industry, Beijing Academy of Science and Technology, Beijing, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002645:Chickens; D058266:Dielectric Spectroscopy; D004528:Egg Shell / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D004583:Electrons; D005898:Glass; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008566:Membranes; D053768:Metal Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D015203:Reproducibility of Results; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002244:Carbon; D006046:Gold; D006861:Hydrogen Peroxide