title: MicroRNA-9 Regulates the Differentiation and Function of Myeloid-Derived Suppressor Cells via Targeting Runx1.
abstract: Myeloid-derived suppressor cells (MDSCs) play a critical role in tumor-associated immunosuppression, thus affecting effective immunotherapies for cancers. However, the molecular mechanisms involved in regulating the differentiation and function of MDSCs remain largely unclear. In this study, we found that inhibition of microRNA (miR)-9 promoted the differentiation of MDSCs with significantly reduced immunosuppressive function whereas overexpression of miR-9 markedly enhanced the function of MDSCs. Notably, knockdown of miR-9 significantly impaired the activity of MDSCs and inhibited the tumor growth of Lewis lung carcinoma in mice. Moreover, miR-9 regulated MDSCs differentiation by targeting the runt-related transcription factor 1, an essential transcription factor in regulating MDSC differentiation and function. Furthermore, the CREB was found to regulate miR-9 expression in MDSCs. Taken together, our findings have identified a critical role of miR-9 in regulating the differentiation and function of MDSCs.
authors.affiliation: Department of Laboratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002, China; Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Ke
authors.initials: K
authors.lastname: Rui
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tang
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Yungang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Laboratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002, China;
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Pathology, University of Hong Kong, Hong Kong 999077, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Laboratory Medicine, Affiliated People's Hospital, Jiangsu University, Zhenjiang 212002, China; Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang 210013, China; and sjwjs@ujs.edu.cn.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D050676:Core Binding Factor Alpha 2 Subunit / Q000096:biosynthesis / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D022423:Myeloid Cells / Q000166:cytology* / Q000276:immunology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D047071:beta-Glucans / Q000494:pharmacology
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D035683:MicroRNAs; C541873:Mirn92 microRNA, mouse; C493726:Runx1 protein, mouse; D047071:beta-Glucans