title: Changes in gene transcription and taxane production in elicited cell cultures of Taxus × media and Taxus globosa.
abstract: The response of two Taxus cell systems to the action of cyclodextrin (CD) and coronatine (CORO), supplied to the culture medium either separately or together, was studied. Two-stage Taxus globosa and Taxus media cell cultures were established and the elicitors were added at the beginning of the second stage. Growth, taxane production, and the expression of known taxol biosynthetic genes, including the recently characterized CoA ligase gene, were studied. Although CORO reduced the growth capacity of both cell lines, CD apparently counteracted this negative effect. Taxane production was significantly enhanced by the simultaneous addition of CD and CORO to the medium. The total taxane production in the T. media cell line was more than double that of T. globosa, but in the latter more than 90% of the taxanes produced were excreted to the medium. Individual taxane patterns also differed: at the height of production, the main taxanes in T. globosa cultures were cephalomannine and 10-deacetyltaxol, and in T. media, taxol and baccatin III. The low transcript levels of taxane biosynthetic genes found in T. globosa cells mirrored the lower taxane production in these cultures, while a high expression was strongly correlated with a high taxane production in T. media.
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Karla
authors.initials: K
authors.lastname: Ramirez-Estrada
authors.affiliation: Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Morelos, Mexico.
authors.forename: Lidia
authors.initials: L
authors.lastname: Osuna
authors.affiliation: Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Moyano
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Bonfill
authors.affiliation: Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Morelos, Mexico.
authors.forename: Nadia
authors.initials: N
authors.lastname: Tapia
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Cusido
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain. Electronic address: javierpalazon@ub.edu.
authors.forename: Javier
authors.initials: J
authors.lastname: Palazon
mesh_terms: D000470:Alkaloids; D000596:Amino Acids; D001952:Bridged-Ring Compounds / Q000032:analysis; D018929:Cell Culture Techniques; D003505:Cyclodextrins / Q000378:metabolism; D007192:Indenes; D017239:Paclitaxel / Q000032:analysis; D043823:Taxoids / Q000032:analysis* / Q000737:chemistry; D020946:Taxus / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D001952:Bridged-Ring Compounds; D003505:Cyclodextrins; D007192:Indenes; D043823:Taxoids; C080625:taxane; C073950:baccatin III; C058487:coronatine; C056348:cephalomannine; C031494:10-deacetyltaxol; D017239:Paclitaxel
keywords: Plant cell cultures; Taxanes; Taxol; Taxus globosa; Taxus×media; Transcription profiles