title: Effect of high fat diet on artificial oocyte activation following superovulation in mice.
abstract: The aim of the present study was to determine the effects of increased dietary intake and high fat diet (HFD) in mice on artificial oocyte activation by using puromycin or roscovitine. Six-week-old mice were fed as either a control diet group, an increased dietary intake group or an HFD group for 4 weeks. Oocytes were obtained following superovulation and were divided into three treatment groups (no activation treatment, calcium ionophore and puromycin treatment, and calcium ionophore and roscovitine treatment) and were incubated for 4 h. Retrieved oocytes and numbers of oocytes activated as assessed by morphological changes were compared among the three treatment groups. The proportion of degenerated oocytes in HFD mice was significantly higher than that in control diet mice. The rates of activation in oocytes treated with roscovitine were 90.3% in control diet mice, 89.8% in increased dietary intake mice and 67.9% in HFD mice. The rate of activation in oocytes treated with roscovitine in HFD mice was significantly lower than the rates in control diet mice and increased dietary intake mice. The rates of activation in oocytes treated with puromycin were 90.6% in control diet mice, 94.0% in increased dietary intake mice and 71.4% in HFD mice, and the rate of activation in oocytes treated with puromycin in HFD mice was significantly lower than the rates in control diet mice and increased dietary intake mice. HFD-induced obesity deteriorated induction of oocyte activation by roscovitine or puromycin in mice.
authors.affiliation: Graduate School of Health Science,University of Tokushima Graduate School,3-18-15 Kuramoto,Tokushima 770-8503,Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamamoto
authors.affiliation: Department of Reproductive Technology,Institute of Health Biosciences,University of Tokushima Graduate School,3-18-15 Kuramoto,Tokushima 770-8503,Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yasui
authors.affiliation: Department of Obstetrics and Gynecology,Institute of Health Biosciences,University of Tokushima Graduate School,3-18-15 Kuramoto,Tokushima 770-8503,Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Kobayashi
authors.affiliation: Department of Obstetrics and Gynecology,Institute of Health Biosciences,University of Tokushima Graduate School,3-18-15 Kuramoto,Tokushima 770-8503,Japan.
authors.forename: Takane
authors.initials: T
authors.lastname: Kitazato
authors.affiliation: School of Health Sciences,University of Tokushima,3-18-15 Kuramoto,Tokushima 770-8503,Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Iwasa
authors.affiliation: School of Health Sciences,University of Tokushima,3-18-15 Kuramoto,Tokushima 770-8503,Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Irahara
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D061207:Calcium Ionophores / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D051379:Mice; D009765:Obesity / Q000097:blood / Q000209:etiology / Q000503:physiopathology; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011687:Purines / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology*; D000077546:Roscovitine; D013480:Superovulation / Q000502:physiology; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D061207:Calcium Ionophores; D005230:Fatty Acids, Nonesterified; D011687:Purines; D014280:Triglycerides; D000077546:Roscovitine; D011691:Puromycin; D002784:Cholesterol
keywords: High fat diet; Oocyte activation; Puromycin; Roscovitine