title: [Multilocus sequence typing analysis of enteropathogenic escherichia coli isolates in 8 provinces of China, 2006-2014].
abstract: OBJECTIVE
  To investigate the molecular typing feature of enteropathogenic Escherichia coli (EPEC) strains isolated from different reservoirs in eight provinces of China from 2006 to 2014.
  
  
  METHODS
  According to the time, place, reservoir, and PFGE pattern of the EPEC strains isolated from stools of humans with diarrhea, animal feces, and foods in eight provinces of China between 2006 and 2014, 149 EPEC strains were selected and characterized by multilocus sequence typing (MLST) using seven housekeeping genes provided by E.coli MLST database. Strain analysis demonstrated 56 different sequence types (STs). SeqMan II, MEGA 5.05, and eBURST V3 were applied to analyze the genetic relationships of domestic and forein existing 392 strains (243 EPEC strains included in the E.coli MLST database and 149 EPEC strains comprised in the present study).
  
  
  RESULTS
  Among the 56 different STs, the prevalent ST was ST-40, which included 19 (19/149, 12.8%) isolates. Nineteen new STs were identified. Eleven new alleles were detected in six house-keeping genes (adk, fumC, gyrB, icd, mdh, and purA). Six STs were simultaneously detected among EPEC strains isolated from patients with diarrhea and animals. And these EPEC strains were all aEPEC strains. Two STs were simultaneously identified among EPEC strains isolated from patients with diarrhea and foods. Also, these EPEC strains were all aEPEC strains. 33 out of 173 STs were divided into five major clone complexes by eBURST, STC-29, STC-10, STC-20, STC-28, and STC-517. The remaining EPEC strains included in the other 140 STs were part of the other clone complexes or just were singletons.
  
  
  CONCLUSIONS
  A high degree of phylogenetic heterogeneity was observed among the EPEC strains isolated in eight provinces of China. The EPEC strains with same STs of human isolates isolated from animal feces and foods were all aEPEC strains.
authors.affiliation: Beijing Jishuitan Hospital, Beijing 100035, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Xiangning
authors.initials: X
authors.lastname: Bai
authors.forename: Ailan
authors.initials: A
authors.lastname: Zhao
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Email: xyanmei2006@126.com.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D002681:China; D003967:Diarrhea; D054308:Enteropathogenic Escherichia coli; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005243:Feces; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D029968:Escherichia coli Proteins