title: Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model.
abstract: Genetic vaccination using naked plasmid DNA is an immunization strategy both against infectious diseases and cancer. In order to improve the efficacy of DNA vaccines, particularly in large animals and humans, different strategies have been pursued. These vaccination strategies are based on different application routes, schedules, and coexpression of immunomodulatory molecules as adjuvants. Our mouse tumor model offers the possibility to investigate Her2/neu DNA vaccines in different settings, i.e., intramuscular or intradermal application with or without coexpression of adjuvants. Protection from tumor growth in tumor challenge experiments and both T cell and humoral immune responses against Her2/neu peptides are used as surrogate parameters for vaccine efficacy.
authors.affiliation: Laboratory for Molecular Immunotherapy, Charité University Medicine Berlin and Max-Delbrück-Center for Molecular Medicine, Robert- Rössle Strasse 10, 13125, Berlin, Germany, nguyen@mdc-berlin.de.
authors.forename: Tam
authors.initials: T
authors.lastname: Nguyen-Hoai
authors.forename: Antonio
authors.initials: A
authors.lastname: Pezzutto
authors.forename: Jörg
authors.initials: J
authors.lastname: Westermann
mesh_terms: D000818:Animals; D019470:Biolistics / Q000379:methods*; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D058501:Enzyme-Linked Immunospot Assay; D006801:Humans; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000276:immunology* / Q000628:therapy*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology; D016896:Treatment Outcome; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D004247:DNA; D018719:Erb-b2 Receptor Tyrosine Kinases; D019444:Vaccines, DNA