title: Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis.
abstract: Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chi Mei Medical Center, Tainan 735, Taiwan; School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Yen
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Wu
authors.affiliation: Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan; College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Chao-Nan
authors.initials: CN
authors.lastname: Hsiung
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Min-I
authors.initials: MI
authors.lastname: Yeh
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Lin
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan; Department of Dentistry, Wan Fang Hospital, Taipei Medical University, Taipei 110, Taiwan. Electronic address: seanlee@tmu.edu.tw.
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Lee
mesh_terms: D002648:Child; D020219:Chondrogenesis / Q000187:drug effects*; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D057230:Limit of Detection; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D010619:Phenazines / Q000737:chemistry
chemical_list: D007211:Indoles; D010619:Phenazines; C007293:DAPI; D002809:Chondroitin Sulfates; D004247:DNA; C009195:safranine T
keywords: Chondrogenesis; DAPI; Glycosaminoglycan; Safranin O; Screening