title: A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells.
abstract: PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Mei
authors.affiliation: 1] Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA [2] Department of Thoracic Medical Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
authors.forename: Priti
authors.initials: P
authors.lastname: Kumari
authors.affiliation: Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
authors.forename: Amol Carl
authors.initials: AC
authors.lastname: Shetty
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA.
authors.forename: David
authors.initials: D
authors.lastname: Clark
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: Gable
authors.affiliation: 1] Computer-Aided Drug Design Center, Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland 21201, USA [2] Marlene and Stewart Greenebaum Cancer Center, University of Maryland, 22S Greene Street, Baltimore, Maryland 21201, USA.
authors.forename: Alexander D
authors.identifier: 000000034290182X
authors.initials: AD
authors.lastname: MacKerell
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA.
authors.forename: Mark Z
authors.initials: MZ
authors.lastname: Ma
authors.affiliation: 1] Marlene and Stewart Greenebaum Cancer Center, University of Maryland, 22S Greene Street, Baltimore, Maryland 21201, USA [2] Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Weber
authors.affiliation: Marlene and Stewart Greenebaum Cancer Center, University of Maryland, 22S Greene Street, Baltimore, Maryland 21201, USA.
authors.forename: Austin J
authors.initials: AJ
authors.lastname: Yang
authors.affiliation: Marlene and Stewart Greenebaum Cancer Center, University of Maryland, 22S Greene Street, Baltimore, Maryland 21201, USA.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Edelman
authors.affiliation: 1] Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA [2] Marlene and Stewart Greenebaum Cancer Center, University of Maryland, 22S Greene Street, Baltimore, Maryland 21201, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Mao
mesh_terms: D000199:Actins / Q000378:metabolism*; D015152:Blotting, Northern; D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D000099149:Ezrin
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D017923:Sodium-Hydrogen Exchangers; D000099149:Ezrin; C093376:sodium-hydrogen exchanger regulatory factor; C071058:moesin; C071776:radixin