title: Polyphenol administration impairs T-cell proliferation by imprinting a distinct dendritic cell maturational profile.
abstract: Currently little is known as to how nutritionally derived compounds may affect dendritic cell (DC) maturation and potentially prevent inappropriate inflammatory responses that are characteristic of chronic inflammatory syndromes. Previous observations have demonstrated that two polyphenols quercetin and piperine delivered through reconstituted oil bodies (ROBs-QP) can influence DC maturation in response to LPS leading to a modulated inflammatory response. In the present study, we examined the molecular effects of ROBs-QP exposure on DC differentiation in mice and identified a unique molecular signature in response to LPS administration that potentially modulates DC maturation and activity in inflammatory conditions. Following LPS administration, ROBs-QP-exposed DCs expressed an altered molecular profile as compared with control DCs, including cytokine and chemokine production, chemokine receptor repertoire, and antigen presentation ability. In vivo ROBs-QP administration suppresses antigen-specific T-cell division in the draining lymph nodes resulting from a reduced ability to create stable immunological synapse. Our data demonstrate that polyphenols exposure can drive DCs toward a new anti-inflammatory molecular profile capable of dampening the inflammatory response, highlighting their potential as complementary nutritional approaches in the treatment of chronic inflammatory syndromes.
authors.affiliation: IRCCS "de Bellis", Laboratory of Experimental Immunopathology, Castellana Grotte, BA, Italy.
authors.forename: Francesca Romana
authors.initials: FR
authors.lastname: Delvecchio
authors.affiliation: IRCCS "de Bellis", Laboratory of Experimental Immunopathology, Castellana Grotte, BA, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Vadrucci
authors.affiliation: IRCCS "de Bellis", Laboratory of Experimental Immunopathology, Castellana Grotte, BA, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cavalcanti
authors.affiliation: IRCCS "de Bellis", Laboratory of Experimental Immunopathology, Castellana Grotte, BA, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: De Santis
authors.affiliation: School of Health Science, University of Tasmania, Launceston, Tasmania, Australia.
authors.forename: Dale
authors.initials: D
authors.lastname: Kunde
authors.affiliation: Mario Negri Sud, Laboratory of Lipid Metabolism and Cancer, Santa Maria Imbaro, CH, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Vacca
authors.affiliation: Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors.forename: Jay
authors.initials: J
authors.lastname: Myers
authors.affiliation: Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors.forename: Frederick
authors.initials: F
authors.lastname: Allen
authors.affiliation: IRCCS "de Bellis", Laboratory of Experimental Immunopathology, Castellana Grotte, BA, Italy.
authors.forename: Giusy
authors.initials: G
authors.lastname: Bianco
authors.affiliation: Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors.forename: Alex Y
authors.initials: AY
authors.lastname: Huang
authors.affiliation: Department of Pathology and Diagnostic, University of Verona Medical School, Verona, Italy.
authors.forename: Vladia
authors.initials: V
authors.lastname: Monsurro
authors.affiliation: CNR, Institute of Science of Food Production, Lecce, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Santino
authors.affiliation: IRCCS "de Bellis", Laboratory of Experimental Immunopathology, Castellana Grotte, BA, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Chieppa
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D052117:Benzodioxoles / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D015870:Gene Expression; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010880:Piperidines / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D061251:Primary Cell Culture; D011794:Quercetin / Q000494:pharmacology*; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D016207:Cytokines; D008070:Lipopolysaccharides; D010880:Piperidines; D059808:Polyphenols; D053284:Polyunsaturated Alkamides; D019707:Receptors, Chemokine; D011794:Quercetin; C008922:piperine
keywords: Antigen presentation/processing; Dendritic cells; Inflammation