title: Pim-2 Modulates Aerobic Glycolysis and Energy Production during the Development of Colorectal Tumors.
abstract: Tumor cells have higher rates of glucose uptake and aerobic glycolysis to meet energy demands for proliferation and metastasis. The characteristics of increased glucose uptake, accompanied with aerobic glycolysis, has been exploited for the diagnosis of cancers. Although much progress has been made, the mechanisms regulating tumor aerobic glycolysis and energy production are still not fully understood. Here, we demonstrate that Pim-2 is required for glycolysis and energy production in colorectal tumor cells. Our results show that Pim-2 is highly expressed in colorectal tumor cells, and may be induced by nutrient stimulation. Activation of Pim-2 in colorectal cells led to increase glucose utilization and aerobic glycolysis, as well as energy production. While knockdown of Pim-2 decreased energy production in colorectal tumor cells and increased their susceptibility to apoptosis. Moreover, the effects of Pim-2 kinase on aerobic glycolysis seem to be partly dependent on mTORC1 signaling, because inhibition of mTORC1 activity reversed the aerobic glycolysis mediated by Pim-2. Our findings suggest that Pim-2-mediated aerobic glycolysis is critical for monitoring Warburg effect in colorectal tumor cells, highlighting Pim-2 as a potential metabolic target for colorectal tumor therapy.
authors.affiliation: 1. Daqing Oilfield General Hospital, Zhongkang Street 9, Daqing, 163001, China.
authors.forename: Xue-hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: 1. Daqing Oilfield General Hospital, Zhongkang Street 9, Daqing, 163001, China ; 2. The Second Affiliated Hospital of Harbin Medical University, Road Xuefu 246, Harbin, 150086, China.
authors.forename: Hong-liang
authors.initials: HL
authors.lastname: Yu
authors.affiliation: 2. The Second Affiliated Hospital of Harbin Medical University, Road Xuefu 246, Harbin, 150086, China.
authors.forename: Fu-jing
authors.initials: FJ
authors.lastname: Wang
authors.affiliation: 3. The Sixth People's Affiliated Hospital of Shanghai Jiao Tong University, Road Yishan 600, Shanghai, 200233, China.
authors.forename: Yong-long
authors.initials: YL
authors.lastname: Han
authors.affiliation: 2. The Second Affiliated Hospital of Harbin Medical University, Road Xuefu 246, Harbin, 150086, China.
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Yang
mesh_terms: D000332:Aerobiosis / Q000235:genetics; D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C490587:PIM2 protein, human; D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases
keywords: Aerobic glycolysis; Apoptosis; Pim-2; Warburg effect