title: Cell growth and migration under octenidine-antiseptic treatment.
abstract: OBJECTIVE
  The toxicity of octenidine antiseptics in cultured cells contrasts their good tolerability in tissue. This phenomenon prompted us to examine which cell culture conditions allow survival and proliferation and to investigate a possible modulation of toxicity by the extracellular matrix proteoglycan chondroitin sulfate.
  
  
  METHODS
  We tested fibroblasts and MCF7 cells for growth using the MTT test, and assessed wound healing potency with a laceration assay. Expression levels of the genes involved in controlling wound healing were assessed with RT-PCR.
  
  
  RESULTS
  A 24 hour exposure to the octenidine-based solution was found incompatible with cell growth. When octenidine solution (0.5-0.5mg/l) was coated on dishes, growth was profoundly reduced after 24 hours, however there was no cytotoxic effect at 0.012 mg/l. Interestingly, when dishes were first coated with chondroitin sulfate the cytotoxicity of octenidine-based solution was modulated. Cell migration was not inhibited by octenidine-based solution treatment (2 minutes; 15 mg/l). No significant changes in gene expression levels in response to the octenidine-based solution treatment were detected.
  
  
  CONCLUSIONS
  In cell culture conditions application of the octenidine-based solution without toxicity can be observed, comparable to the minimal application required to give full bactericidal effect. Alteration of toxicity by interaction with chondroitin sulfate in cell culture suggests a similar function for extraceullar matrix in intact tissue.
authors.affiliation: Center for Pathobiochemistry and Medical Genetics, Medical University of Vienna, Austria.|Present address Abteilung für Molekulare Genetik, Medical University Vienna, Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Jenull
authors.affiliation: Center for Pathobiochemistry and Medical Genetics, Medical University of Vienna, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Hojdar
authors.affiliation: Center for Pathobiochemistry and Medical Genetics, Medical University of Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Laggner
authors.affiliation: Center for Pathobiochemistry and Medical Genetics, Medical University of Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Velimirov
authors.affiliation: Center for Pathobiochemistry and Medical Genetics, Medical University of Vienna, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Zemann
authors.affiliation: Center for Pathobiochemistry and Medical Genetics, Medical University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Huettinger
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007097:Imines; D011725:Pyridines / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D007097:Imines; D011725:Pyridines; C034213:octenidine
keywords: cell growth; chondroitin sulfate; migration; octenidine