title: Correction of Mitochondrial Enzyme Activities in the Skeletal Muscles of Old Rats in Response to Addition of Olive Oil to the Ration.
abstract: Activities of mitochondrial electron transport chain enzymes NADH-CoQ oxidoreductase (complex I), cytochrome C-oxidase (complex IV), and citrate synthase were measured by spectrophotometry in m. quadriceps femoris homogenate from old rats receiving olive oil with the ration. Reduced activities of complexes I and IV in old animals were restored to the level of young animals after 6-week consumption of olive oil. Activity of citrate synthase did not change with age. Positive effect of olive oil on fatty-acid composition of the muscle tissue in old animals was demonstrated. The content of summary monounsaturated fatty acids, reduced with aging, and of summary polyunsaturated ones, increasing with age, were restored in old rats to the levels virtually not differing from the levels of young animals.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia, genefrbron@mail.com.
authors.forename: G E
authors.initials: GE
authors.lastname: Bronnikov
authors.forename: T P
authors.initials: TP
authors.lastname: Kulagina
authors.forename: A V
authors.initials: AV
authors.lastname: Aripovskii
authors.forename: L I
authors.initials: LI
authors.lastname: Kramarova
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D004032:Diet; D042967:Electron Transport Complex I / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D000069463:Olive Oil / Q000494:pharmacology*; D052097:Quadriceps Muscle / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry
chemical_list: D005229:Fatty Acids, Monounsaturated; D000069463:Olive Oil; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; D042967:Electron Transport Complex I