title: Cardiovascular and thermoregulatory dysregulation over 24 h following acute heat stress in rats.
abstract: The influences of severe heat stroke (HS) on cardiovascular function during recovery are incompletely understood. We hypothesized that HS would elicit a heart rate (HR) increase persisting through 24 h of recovery due to hemodynamic, thermoregulatory, and inflammatory events, necessitating tachycardia to support mean arterial pressure (MAP). Core temperature (Tc), HR, and MAP were measured via radiotelemetry in conscious male Fischer 344 rats (n = 22; 282.4 ± 3.5 g) during exposure to 37°C ambient temperature until a maximum Tc of 42.0°C, and during recovery at 20°C ambient temperature through 24 h. Rats were divided into Mild, Moderate, and Severe groups based on pathophysiology. HS rats exhibited hysteresis relative to Tc with HR higher for a given Tc during recovery compared with heating (P < 0.0001). "Reverse" hysteresis occurred in MAP with pressure during cooling lower than heating per degree Tc (P < 0.0001). Mild HS rats showed tachycardia [P < 0.01 vs. control (Con)] through 8 h of recovery, elevated MAP (P < 0.05 vs. Con) for the initial 5 h of recovery, with sustained hyperthermia (P < 0.05 vs. Con) through 24 h. Moderate HS rats showed significant tachycardia (P < 0.01 vs. Con), normal MAP (P > 0.05 vs. Con), and rebound hyperthermia from 4 to 24 h post-HS (P < 0.05 vs. Con). Severe HS rats showed tachycardia (P < 0.05 vs. Con), hypotension (P < 0.01 vs. Con), and hypothermia for 24 h (P < 0.05 vs. Con). Severe HS rats showed 14- and 12-fold increase in heart and liver inducible nitric oxide synthase expression, respectively. Hypotension and hypothermia in Severe HS rats was consistent with inducible nitric oxide synthase-mediated systemic vasodilation. These findings provide mechanistic insight into hemodynamic and thermoregulatory impairments during 24 h of HS recovery.
authors.affiliation: US Army Research Institute of Environmental Medicine, Thermal and Mountain Medicine Division, Natick, Massachusetts carrie.m.quinn.mil@mail.mil.
authors.forename: Carrie M
authors.initials: CM
authors.lastname: Quinn
authors.affiliation: US Army Research Institute of Environmental Medicine, Thermal and Mountain Medicine Division, Natick, Massachusetts.
authors.forename: Gerald N
authors.initials: GN
authors.lastname: Audet
authors.affiliation: US Army Research Institute of Environmental Medicine, Thermal and Mountain Medicine Division, Natick, Massachusetts.
authors.forename: Nisha
authors.initials: N
authors.lastname: Charkoudian
authors.affiliation: US Army Research Institute of Environmental Medicine, Thermal and Mountain Medicine Division, Natick, Massachusetts.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Leon
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001833:Body Temperature Regulation; D006339:Heart Rate; D018883:Heat Stroke / Q000503:physiopathology*; D018869:Heat-Shock Response; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D014664:Vasodilation
keywords: autonomic nervous system; blood pressure; heart rate; heat stroke; iNOS; thermoregulation