title: A Modular Approach To Study Protein Adsorption on Surface Modified Hydroxyapatite.
abstract: Biocompatible inorganic nano- and microcarriers can be suitable candidates for protein delivery. This study demonstrates facile methods of functionalization by using nanoscale linker molecules to change the protein adsorption capacity of hydroxyapatite (HA) powder. The adsorption capacity of bovine serum albumin as a model protein has been studied with respect to the surface modifications. The selected linker molecules (lysine, arginine, and phosphoserine) can influence the adsorption capacity by changing the electrostatic nature of the HA surface. Qualitative and quantitative analyses of linker-molecule interactions with the HA surface have been performed by using NMR spectroscopy, zeta-potential measurements, X-ray photoelectron spectroscopy, and thermogravimetric analyses. Additionally, correlations to theoretical isotherm models have been calculated with respect to Langmuir and Freundlich isotherms. Lysine and arginine increased the protein adsorption, whereas phosphoserine reduced the protein adsorption. The results show that the adsorption capacity can be controlled with different functionalization, depending on the protein-carrier selections under consideration. The scientific knowledge acquired from this study can be applied in various biotechnological applications that involve biomolecule-inorganic material interfaces.
authors.affiliation: Department of Chemistry, University of Liège, B6a, Allée de la Chimie 3, Liège 4000 (Belgium).|Sustainable Materials Management, Flemish Institute for Technological Research (VITO), Boeretang 200, Mol 2400 (Belgium).|Present address: Department of Physics, UNESP, Univ. Estadual Paulista, 17033-360, Bauru, SP (Brazil).
authors.forename: Vinayaraj
authors.initials: V
authors.lastname: Ozhukil Kollath
authors.affiliation: Department of Organic Chemistry, Ghent University, Krijgslaan 281, Ghent 9000 (Belgium).
authors.forename: Freya
authors.initials: F
authors.lastname: Van den Broeck
authors.affiliation: Department of Organic Chemistry, Ghent University, Krijgslaan 281, Ghent 9000 (Belgium).
authors.forename: Krisztina
authors.initials: K
authors.lastname: Fehér
authors.affiliation: Department of Organic Chemistry, Ghent University, Krijgslaan 281, Ghent 9000 (Belgium).
authors.forename: José C
authors.initials: JC
authors.lastname: Martins
authors.affiliation: Department of Metallurgy and Materials Engineering, Katholieke Universiteit Leuven, 3001 Heverlee (Belgium).
authors.forename: Jan
authors.initials: J
authors.lastname: Luyten
authors.affiliation: APTIS, University of Liège, B5a, Allée de la Chimie 3, Liège 4000 (Belgium).|Present address: Galephar MF, 6900 Marche en Famenne (Belgium).
authors.forename: Karl
authors.initials: K
authors.lastname: Traina
authors.affiliation: Sustainable Materials Management, Flemish Institute for Technological Research (VITO), Boeretang 200, Mol 2400 (Belgium). steven.mullens@vito.be.
authors.forename: Steven
authors.initials: S
authors.lastname: Mullens
authors.affiliation: Department of Chemistry, University of Liège, B6a, Allée de la Chimie 3, Liège 4000 (Belgium). rcloots@ulg.ac.be.
authors.forename: Rudi
authors.initials: R
authors.lastname: Cloots
mesh_terms: D000327:Adsorption; D000818:Animals; D001120:Arginine / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D010768:Phosphoserine / Q000737:chemistry* / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D013499:Surface Properties
chemical_list: D010768:Phosphoserine; D017886:Durapatite; D001120:Arginine; D008239:Lysine
keywords: NMR spectroscopy; adsorption; amino acids; proteins; surface chemistry