title: Mass Spectrometry-Based Proteomic Study Makes High-Density Lipoprotein a Biomarker for Atherosclerotic Vascular Disease.
abstract: High-density lipoprotein (HDL) is a lipid and protein complex that consists of apolipoproteins and lower level HDL-associated enzymes. HDL dysfunction is a factor in atherosclerosis and decreases patient survival. Mass spectrometry- (MS-) based proteomics provides a high throughput approach for analyzing the composition and modifications of complex HDL proteins in diseases. HDL can be separated according to size, surface charge, electronegativity, or apoprotein composition. MS-based proteomics on subfractionated HDL then allows investigation of lipoprotein roles in diseases. Herein, we review recent developments in MS-based quantitative proteomic techniques, HDL proteomics and lipoprotein modifications in diseases, and HDL subfractionation studies. We also discuss future directions and perspectives in MS-based proteomics on HDL.
authors.affiliation: College of Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Division of Nephrology, China Medical University Hospital, 2 Yu-Der Road, Taichung 40447, Taiwan ; L5 Research Center, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chiz-Tzung
authors.identifier: 0000-0002-0306-6676
authors.initials: CT
authors.lastname: Chang
authors.affiliation: L5 Research Center, China Medical University Hospital, Taichung, Taiwan ; Section of Cardiovascular Research, Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors.forename: Chao-Yuh
authors.initials: CY
authors.lastname: Yang
authors.affiliation: Department of Medical Genetics, Pediatrics and Medical Research, China Medical University Hospital, Taichung 40447, Taiwan.
authors.forename: Fuu-Jen
authors.initials: FJ
authors.lastname: Tsai
authors.affiliation: College of Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Division of Nephrology, China Medical University Hospital, 2 Yu-Der Road, Taichung 40447, Taiwan.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan ; Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: Chao-Jung
authors.identifier: 0000-0003-3834-9243
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D001053:Apolipoproteins / Q000378:metabolism; D050197:Atherosclerosis / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001053:Apolipoproteins; D015415:Biomarkers; D008075:Lipoproteins, HDL