title: Temperature-based on-column solute focusing in capillary liquid chromatography reduces peak broadening from pre-column dispersion and volume overload when used alone or with solvent-based focusing.
abstract: On-column focusing is essential for satisfactory performance using capillary scale columns. On-column focusing results from generating transient conditions at the head of the column that lead to high solute retention. Solvent-based on-column focusing is a well-known approach to achieve this. Temperature-assisted on-column focusing (TASF) can also be effective. TASF improves focusing by cooling a short segment of the column inlet to a temperature that is lower than the column temperature during the injection and then rapidly heating the focusing segment to the match the column temperature. A troublesome feature of an earlier implementation of TASF was the need to leave the capillary column unpacked in that portion of the column inside the fitting connecting it to the injection valve. We have overcome that problem in this work by packing the head of the column with solid silica spheres. In addition, technical improvements to the TASF instrumentation include: selection of a more powerful thermo-electric cooler to create faster temperature changes and electronic control for easy incorporation into conventional capillary instruments. Used in conjunction with solvent-based focusing and with isocratic elution, volumes of paraben samples (esters of p-hydroxybenzoic acid) up to 4.5-times the column liquid volume can be injected without significant bandspreading due to volume overload. Interestingly, the shapes of the peaks from the lowest volume injections that we can make, 30nL, are improved when using TASF. TASF is very effective at reducing the detrimental effects of pre-column dispersion using isocratic elution. Finally, we show that TASF can be used to focus the neuropeptide galanin in a sample solvent with elution strength stronger than the mobile phase. Here, the stronger solvent is necessitated by the need to prevent peptide adsorption prior to and during analysis.
authors.affiliation: Department of Chemistry University of Pittsburgh 219 Parkman Avenue Pittsburgh, PA 15260, United States.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Groskreutz
authors.affiliation: Department of Chemistry University of Pittsburgh 219 Parkman Avenue Pittsburgh, PA 15260, United States.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Horner
authors.affiliation: Department of Chemistry University of Pittsburgh 219 Parkman Avenue Pittsburgh, PA 15260, United States. Electronic address: sweber@pitt.edu.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Weber
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019004:Galanin / Q000032:analysis; D012822:Silicon Dioxide; D012997:Solvents; D013696:Temperature
chemical_list: D012997:Solvents; D012822:Silicon Dioxide; D019004:Galanin
keywords: Capillary HPLC; On-column focusing; Temperature; Volume overload