title: Downregulation of microRNA-409-3p promotes aggressiveness and metastasis in colorectal cancer: an indication for personalized medicine.
abstract: BACKGROUND
  MicroRNAs play an essential role in colorectal cancer development and progression. Aberrant miR-409-3p expression has been reported in several cancers. However, the clinical significance and functions of miR-409-3p in human CRC were not entirely clear.
  
  
  METHODS
  miR-409-3p expression levels were determined in 45 pairs of primary CRC and their corresponding adjacent non-tumor tissues by qPCR. The effects of ectopic expression of miR-409-3p on CRC cells proliferation, wound healing, metastasis were investigated by CCK-8, transwell assay and peritoneal spreading nude mice model.
  
  
  RESULTS
  Statistical analysis of clinical cases revealed that low miR-409-3p expression had inclinations towards lager tumor size and local invasion. Ectopic expression of miRNA mimics suggested that miR-409-3p could inhibits the abilities of proliferation, wound healing, metastasis and invasion in CRC cells. Notably, we found the NLK could be a potential target of miR-409-3p.
  
  
  CONCLUSIONS
  Our results suggest that miR-409-3p functions as a tumor suppressor by inhibiting the development and metastasis of CRC, suggesting that miR-409-3p is expected to become a new diagnostic marker and a new target of the treatment of CRC.
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233030, China. mulinliu11@163.com.
authors.forename: Mulin
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230000, China. amanxu@yeah.net.
authors.forename: Aman
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230000, China. xiaoyuanxy@yeah.net.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233030, China. qzhangz@126.com.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233030, China. taotfang@163.com.
authors.forename: Taotao
authors.initials: T
authors.lastname: Fang
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230000, China. nihao2209256@163.com.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, China. lcljm11@163.com.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002999:Clone Cells; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010537:Peritoneum / Q000473:pathology; D057285:Precision Medicine; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D047368:Tumor Burden; D014945:Wound Healing / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D047908:Intracellular Signaling Peptides and Proteins; C572367:MIRN409 microRNA, human; D035683:MicroRNAs; C470551:NLK protein, human; D017346:Protein Serine-Threonine Kinases