title: Experimental Model to Study the Role of Retinoblastoma Gene Product (pRb) for Determination of Adipocyte Differentiation.
abstract: Using stable constitutive expression of retinoblastoma gene product (pRb) in polypotent mesenchymal 10T1/2 cells we obtained stable cell lines hyperexpressing functionally active or inactive mutant pRb. The cells producing active exogenous pRb demonstrated high sensitivity to adipocyte differentiation inductors, whereas production of inactive form of the exogenous protein suppressed adipocyte differentiation. The obtained lines can serve as the experimental model for studying the role of pRb in determination of adipocyte differentiation.
authors.affiliation: Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia, borisvp478@gmail.com.
authors.forename: B V
authors.initials: BV
authors.lastname: Popov
authors.forename: P S
authors.initials: PS
authors.lastname: Shilo
authors.forename: O V
authors.initials: OV
authors.lastname: Zhidkova
authors.forename: A M
authors.initials: AM
authors.lastname: Zaichik
authors.forename: N S
authors.initials: NS
authors.lastname: Petrov
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D015151:Immunoblotting; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D010957:Plasmids / Q000235:genetics; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D016160:Retinoblastoma Protein