title: The isolation and characterization of the novel chlorothalonil-degrading strain Paracoccus sp. XF-3 and the cloning of the chd gene.
abstract: Chlorothalonil (CTN) is one of the most widely used fungicides and is often detected in the environment. Here, we report the isolation and characterization of a novel CTN-degrading bacterial strain XF-3 from long-term CTN-contaminated sites and identify it as a strain of the Paracoccus sp. The isolate could utilise CTN as the sole source of carbon and energy for growth. The optimal pH and temperature for degradation by XF-3 were 7.0 and 30°C, respectively. The CTN degradation gene was cloned by PCR. Although the results of a BLAST sequence search indicated that this gene has a 99% similarity with chd (a gene encoding the CTN hydrolytic dehalogenase), its hydrolytic efficiency for CTN was slightly greater than the chd from strain CTN-3. This is the first report of this gene from the genus Paracoccus. Therefore, there is a practical significance and a potential value of the isolated novel strain, XF-3.
authors.affiliation: Department of Bioengineering, College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui Province, PR China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Yue
authors.affiliation: Department of Bioengineering, College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui Province, PR China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Xiong
authors.affiliation: Department of Bioengineering, College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui Province, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Bioengineering, College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui Province, PR China. Electronic address: wanf-3344@163.com.
authors.forename: Guangli
authors.initials: G
authors.lastname: Wang
mesh_terms: D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D005659:Fungicides, Industrial / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009570:Nitriles / Q000378:metabolism*; D010230:Paracoccus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013696:Temperature
chemical_list: D005659:Fungicides, Industrial; D009570:Nitriles; C005806:tetrachloroisophthalonitrile
keywords: Biodegradation; Chlorothalonil; Paracoccus sp.; Strain XF-3; chd gene