title: Delayed tooth replantation following root canal filling with calcium hydroxide and MTA: Histomorphometric study in rats.
abstract: OBJECTIVE
  The aim of this study was to perform a histomorphometric evaluation of the repair process in rat teeth replanted after root canals were filled with calcium hydroxide (CH) and mineral trioxide aggregate (MTA).
  
  
  METHODS
  Upper right incisors were extracted from 30 rats divided into three groups (n=10). The teeth were stored dry for 60min, after which the pulp and periodontal ligament (PDL) were removed and immersed in acidulated-phosphate sodium fluoride solution. In Group I, the root canals were filled with saline; in Group II, they were filled with CH; and in Group III, they were filled with CH, and the foramen was sealed with an MTA plug. The teeth were replanted, and the animals were sacrificed after 60 days. The sections with teeth were removed for histological preparation (haematoxylin and eosin, H&E). The characteristics of the PDL, cementum, dentine, and alveolar bone, as well as the occurrence of inflammatory and replacement root resorption and apical sealing, were subjected to histological and morphometric analysis (P<0.05).
  
  
  RESULTS
  Group I was the most affected by root resorption (mean=67.05%). In Groups II and III, the resorption averaged 42.2% and 11.7%, respectively. Group III was less affected by inflammatory resorption and presented more areas of apical sealing by mineralized tissue (P<0.05).
  
  
  CONCLUSIONS
  An apical MTA plug improved the repair of the replanted tooth by decreasing surface resorption and repairing mineralized tissue in the periapical region.
authors.affiliation: Federal University of Rio de Janeiro, UFRJ, Dental School, R. Prof Rodolpho Paulo Rocco 325/2o Andar, Ilha da Cidade Universitária, Rio de Janeiro, RJ CEP 21941-913, Brazil. Electronic address: jonatasce@hotmail.com.
authors.forename: Jônatas Caldeira
authors.initials: JC
authors.lastname: Esteves
authors.affiliation: Univ Estadual Paulista-UNESP-Araçatuba Dental School, Rua José Bonifácio, 1193, Vila Mendonç, Zip Code 16015-050, Araçatuba, SP, Brazil.
authors.forename: Heloisa Fonseca
authors.initials: HF
authors.lastname: Marão
authors.affiliation: Faculdades Integradas Aparício Carvalho, FIMCA, Ruas das Araras, 241, Eldorado, Porto Velho, Rondônia, Brazil.
authors.forename: Pedro Ivo Dos Santos
authors.initials: PI
authors.lastname: Silva
authors.affiliation: Univ Estadual Paulista-UNESP-Araçatuba Dental School, Rua José Bonifácio, 1193, Vila Mendonç, Zip Code 16015-050, Araçatuba, SP, Brazil.
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Poi
authors.affiliation: Univ Estadual Paulista-UNESP-Araçatuba Dental School, Rua José Bonifácio, 1193, Vila Mendonç, Zip Code 16015-050, Araçatuba, SP, Brazil.
authors.forename: Sônia Regina
authors.initials: SR
authors.lastname: Panzarini
authors.affiliation: Univ Estadual Paulista-UNESP-Araçatuba Dental School, Rua José Bonifácio, 1193, Vila Mendonç, Zip Code 16015-050, Araçatuba, SP, Brazil.
authors.forename: Alessandra Marcondes
authors.initials: AM
authors.lastname: Aranega
authors.affiliation: Univ Estadual Paulista-UNESP-Araçatuba Dental School, Rua José Bonifácio, 1193, Vila Mendonç, Zip Code 16015-050, Araçatuba, SP, Brazil.
authors.forename: Eduardo Dias
authors.initials: ED
authors.lastname: Ribeiro
authors.affiliation: Univ Estadual Paulista-UNESP-Araçatuba Dental School, Rua José Bonifácio, 1193, Vila Mendonç, Zip Code 16015-050, Araçatuba, SP, Brazil.
authors.forename: Celso Koogi
authors.initials: CK
authors.lastname: Sonoda
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D004338:Drug Combinations; D007180:Incisor / Q000187:drug effects / Q000473:pathology; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012391:Root Resorption / Q000473:pathology / Q000517:prevention & control*; D017640:Silicates / Q000494:pharmacology*; D014081:Tooth Extraction; D014090:Tooth Replantation / Q000379:methods*; D014092:Tooth Root / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D002126:Calcium Hydroxide
keywords: Calcium hydroxide; Mineral trioxide aggregate; Periodontal ligament; Root resorption; Tooth replantation