title: Microfluidic system for high-throughput immunoglobulin-E analysis from clinical serum samples.
abstract: Rapid and high-throughput analytical techniques for IgE that requires a small serum amount are very important, especially for pediatric patients. In these patients, blood is collected from veins, which is painful compared to fingertip blood collection. Herein, a novel microfluidic system capable of high-throughput parallel analyses of allergen-specific IgE from small amounts of patient serum was successfully developed. A six-plex immunoassay was constructed within a microfluidic chip, and the entire system was validated using samples from clinical patients. Major antigens from house dust mite (Dermatophagoides farinae and Blomia tropicalis), cat (Felis domesticus), fungus (Cladosporium herbarum), ragweed (Humulus japonicas), and tree pollen (Platanus acerifolia) were used as analysis targets. Sample consumption decreased to <0.05 µL compared with the 480µL serum consumption by fluoroenzyme immunoassay (UniCAP system Pharmacia Diagnostics AB, Uppsala, Sweden), the 50 µL serum consumption by enzyme-linked immune sorbent assay (ELISA), or the 1.5 µL serum consumption by conventional protein chip analysis. Analysis duration, reagent cost, and total cost for each measurement were also considerably decreased. The assay showed good accuracy and sensitivity toward the clinical samples. A significant correlation of allergen-specific IgE levels was found among the microfluidic assay, UniCAP system, and ELISA.
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
authors.forename: Xiran
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Suqin
authors.initials: S
authors.lastname: Man
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ran
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Feng
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
authors.forename: Sixiu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China; Institute of Biomedical Science, Fudan University, Shanghai 200433, PR China. Electronic address: xjcheng@shmu.edu.cn.
authors.forename: Xunjia
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, PR China; Institute of Biomedical Science, Fudan University, Shanghai 200433, PR China. Electronic address: gsui@fudan.edu.cn.
authors.forename: Guodong
authors.initials: G
authors.lastname: Sui
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D056656:Lab-On-A-Chip Devices
chemical_list: D000941:Antigens; D007073:Immunoglobulin E
keywords: Array; Asthma; Automation; Immunoglobulin-E; Microfluidic