title: Specificity of the Immunomodulating Activity of Sasa veitchii (Japanese Folk Medicine Kumazasa) to Fungal Polysaccharides.
abstract: Many plant extracts are used as well-known folk medicines and exhibit various biological activities that are beneficial to human health. These extracts contain polysaccharides, and some are pathogen-associated molecular patterns (PAMPs) that stimulate innate as well as acquired immune systems. In the present study, the cooperative effects of PAMPs and bamboo water-soluble methanol precipitation (BWMP) in a macromolecular fraction of the hot water extract of Sasa veitchii (in Japanese folk medicine, known as Kumazasa; family Poaceae) were analyzed in vitro using the spleen or bone marrow cells of mice. The splenocytes of male DBA/2 and C57BL/6 mice were cultured with BWMP in the presence or absence of PAMPs, and responses were assessed by measuring cytokines. BWMP inhibited the production of interferon gamma (IFN-γ) by not only toll like receptors (TLRs), but also the C-type lectin receptors (CLRs) dectin-1 and dectin-2. BWMP also inhibited the autologous production of IFN-γ in the splenocyte culture. These results suggested that BWMP may inhibit the signaling pathways of PAMPs, but not ligand-receptor interactions. In contrast, BWMP did not inhibit the production of cytokines by dendritic cells. These results indicated that the inhibition of IFN-γ by BWMP was mediated through the cell-to-cell interactions of splenic cells during cultivation.
authors.affiliation: Tokyo University of Pharmacy and Life Science, Tokyo.
authors.forename: Wataru
authors.initials: W
authors.lastname: Sato
authors.affiliation: Hoshi Pharmaceutical Co., Ltd, Tokyo.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takeshita
authors.affiliation: Hoshi Pharmaceutical Co., Ltd, Tokyo.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Tsuboi
authors.affiliation: Hoshi Pharmaceutical Co., Ltd, Tokyo.
authors.forename: Masato
authors.initials: M
authors.lastname: Kanamori
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ishibashi
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392.
authors.forename: Noriko N
authors.initials: NN
authors.lastname: Miura
authors.affiliation: Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.affiliation: Tokyo University Pharmaceutical and Life Sciences, School of Pharmacy, Laboratory Immunopharmacological Microbiological Production.
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects*; D037181:Lectins, C-Type / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018448:Models, Immunological; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031787:Sasa / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D051193:Toll-Like Receptors / Q000187:drug effects / Q000378:metabolism; D000099181:Dectin-1
chemical_list: D016207:Cytokines; D062610:Fungal Polysaccharides; D037181:Lectins, C-Type; D000069452:Pathogen-Associated Molecular Pattern Molecules; D010936:Plant Extracts; D051193:Toll-Like Receptors; D000099181:Dectin-1; C445340:dectin-2, mouse