title: High levels of Porphyromonas gingivalis-induced immunoglobulin G2 are associated with lower high-density lipoprotein levels in chronic periodontitis.
abstract: OBJECTIVE
  To investigate the association between the presence of Porphyromonas gingivalis-induced immunoglobulin G antibodies and the high-density lipoprotein (HDL) level.
  
  
  METHODS
  A total of 108 individuals were examined. The presence of P. gingivalis was detected using primers designed to target the 16S rRNA gene sequence. Peripheral blood was collected from each subject to determine the levels of P. gingivalis-induced IgG1 and IgG2 serum antibodies. The HDL levels were determined using fully enzymatic methods.
  
  
  RESULTS
  A higher proportion of periodontitis patients had high levels of P. gingivalis-induced IgG1 and IgG2, and the proportion of subjects with a HDL level of < 35 md/dL was higher in the group of chronic periodontitis patients. In the unadjusted regression model, the presence of high levels of P. gingivalis-induced IgG2 was associated with a HDL level of < 35 md/dL. The adjusted model indicated that periodontitis patients with high levels of P. gingivalis-induced IgG2 showed 3.2 more chances of having pathological HDL levels (odds ratio = 3.2, 95% confidence interval = 1.2-9.8).
  
  
  CONCLUSIONS
  High levels of P. gingivalis-induced IgG2 were associated with low HDL concentrations in patients with periodontitis, which suggests that the response of the host to periodontal infection may play an important role in the pathogenesis of cardiovascular diseases.
authors.affiliation: Biomedical Stomatology Research Group, Universidad de Antioquia U de A, Medellín, Colombia. martin.ardila@udea.edu.co.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Ardila
authors.affiliation: Biomedical Stomatology Research Group, Universidad de Antioquia U de A, Medellín, Colombia.
authors.forename: Isabel C
authors.initials: IC
authors.lastname: Guzmán
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D055113:Chronic Periodontitis / Q000097:blood* / Q000382:microbiology; D003105:Colombia; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010518:Periodontitis / Q000097:blood / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D015415:Biomarkers; D007074:Immunoglobulin G; D008075:Lipoproteins, HDL; D012336:RNA, Ribosomal, 16S
keywords: antibodies; chronic periodontitis; high-density lipoproteins; immunoglobulin G; periodontitis