title: Protein Production with a Pichia pastoris OCH1 Knockout Strain in Fed-Batch Mode.
abstract: The methylotrophic yeast Pichia pastoris is a widely used host organism for recombinant protein production in biotechnology and pharmaceutical industry. However, if the target product describes a glycoprotein, an α-1,6-mannosyltransferase located in the Golgi apparatus of P. pastoris, called OCH1, triggers hypermannosylation of the recombinant protein which significantly impedes following unit operations and hampers biopharmaceutical product applications. A knockout of the och1 gene allows the production of less-glycosylated proteins-however, morphology and physiology of P. pastoris also change, complicating the upstream process. Here, we describe a controlled and efficient bioprocess based on the specific substrate uptake rate (q s) for a recombinant P. pastoris OCH1 knockout strain expressing a peroxidase as model protein.
authors.affiliation: Research Area Biochemical Engineering, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Gmeiner
authors.forename: Oliver
authors.initials: O
authors.lastname: Spadiut
mesh_terms: D001709:Biotechnology / Q000379:methods; D005656:Fungal Proteins / Q000235:genetics*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000235:genetics; D008364:Mannosyltransferases / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D008364:Mannosyltransferases