title: Bacillus subtilis SalA is a phosphorylation-dependent transcription regulator that represses scoC and activates the production of the exoprotease AprE.
abstract: Bacillus subtilis Mrp family protein SalA has been shown to indirectly promote the production of the exoprotease AprE by inhibiting the expression of scoC, which codes for a repressor of aprE. The exact mechanism by which SalA influences scoC expression has not been clarified previously. We demonstrate that SalA possesses a DNA-binding domain (residues 1-60), which binds to the promoter region of scoC. The binding of SalA to its target DNA depends on the presence of ATP and is stimulated by phosphorylation of SalA at tyrosine 327. The B. subtilis protein-tyrosine kinase PtkA interacts specifically with the C-terminal domain of SalA in vivo and in vitro and is responsible for activating its DNA binding via phosphorylation of tyrosine 327. In vivo, a mutant mimicking phosphorylation of SalA (SalA Y327E) exhibited a strong repression of scoC and consequently overproduction of AprE. By contrast, the non-phosphorylatable SalA Y327F and the ΔptkA exhibited the opposite effect, stronger expression of scoC and lower production of the exoprotease. Interestingly, both SalA and PtkA contain the same ATP-binding Walker domain and have thus presumably arisen from the common ancestral protein. Their regulatory interplay seems to be conserved in other bacteria.
authors.affiliation: Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden.
authors.forename: Abderahmane
authors.initials: A
authors.lastname: Derouiche
authors.affiliation: Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Micalis UMR1319, Institut National de la Recherche Agronomique, Jouy-en-Josas, 78350, France.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bidnenko
authors.affiliation: Micalis UMR1319, Institut National de la Recherche Agronomique, Jouy-en-Josas, 78350, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Ventroux
authors.affiliation: Micalis UMR1319, Institut National de la Recherche Agronomique, Jouy-en-Josas, 78350, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pigonneau
authors.affiliation: Proteome Center Tübingen, University of Tübingen, Tübingen, 72076, Germany.
authors.forename: Mirita
authors.initials: M
authors.lastname: Franz-Wachtel
authors.affiliation: Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden.
authors.forename: Aida
authors.initials: A
authors.lastname: Kalantari
authors.affiliation: Institut de Biochimie et Biophysique Moléculaire et Cellulaire, University Paris-Sud, Orsay, 91405, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Nessler
authors.affiliation: Micalis UMR1319, Institut National de la Recherche Agronomique, Jouy-en-Josas, 78350, France.
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Noirot-Gros
authors.affiliation: Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden.
authors.forename: Ivan
authors.initials: I
authors.lastname: Mijakovic
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D020689:Exopeptidases / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D011494:Protein Kinases / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: C088074:AprE protein, Bacteria; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D026901:Membrane Transport Proteins; C442131:ScoC protein, Bacillus subtilis; D014157:Transcription Factors; D014443:Tyrosine; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D020689:Exopeptidases