title: Distribution of Peripheral Lymphocyte Populations in Primary Sjögren's Syndrome Patients.
abstract: Purpose of this study was to evaluate the lymphocyte populations' distribution changes in peripheral blood of patients with primary Sjögren's syndrome (pSS). Lymphocyte populations' distribution changes in peripheral blood of pSS patients were investigated in 52 patients with pSS and in 28 healthy controls by flow cytometry. We found decreased absolute count of CD3(+) T cell population in pSS patients. Analysis of CD4(+) T cell population showed significant proportion and absolute count differences in pSS patient's blood with SSA/SSB antibodies (Abs) in comparison to controls. No significant differences were observed analyzing CD4(+) and CD8(+) Treg subpopulation. Proportion and absolute counts of Th17 cells were significantly lower in pSS patient's blood. Absolute counts of CD8(+) T cells were significantly lower in pSS patients in comparison to controls and also impaired proportion and absolute counts of CD8(+) subpopulations according to CD27(+) and CD57(+) were observed. Absolute counts of NKT and NK cells were decreased in pSS with Abs. B cells proportion was increased only in blood of pSS with Abs. Lymphocyte distribution impairment can be due to genetically determined lymphopenia or lymphocyte migration from periphery to inflammatory sites or/and increased susceptibility to apoptosis.
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, LT-08409 Vilnius, Lithuania.
authors.forename: Gintaras
authors.initials: G
authors.lastname: Sudzius
authors.affiliation: Department of Innovative Diagnostic Treatment and Health Monitoring Technology, State Research Institute Centre for Innovative Medicine, LT-01102 Vilnius, Lithuania.
authors.forename: Diana
authors.initials: D
authors.lastname: Mieliauskaite
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, LT-08409 Vilnius, Lithuania.
authors.forename: Almantas
authors.identifier: 0000-0002-3089-1428
authors.initials: A
authors.lastname: Siaurys
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, LT-08409 Vilnius, Lithuania.
authors.forename: Rita
authors.initials: R
authors.lastname: Viliene
authors.affiliation: Department of Innovative Diagnostic Treatment and Health Monitoring Technology, State Research Institute Centre for Innovative Medicine, LT-01102 Vilnius, Lithuania ; Faculty of Medicine, Vilnius University, LT-03101 Vilnius, Lithuania.
authors.forename: Irena
authors.initials: I
authors.lastname: Butrimiene
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, LT-08409 Vilnius, Lithuania ; Department of Pathology, Forensic Medicine and Pharmacology, Faculty of Medicine, Vilnius University, LT-03101 Vilnius, Lithuania.
authors.forename: Dainius
authors.initials: D
authors.lastname: Characiejus
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, LT-08409 Vilnius, Lithuania ; Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, LT-10223 Vilnius, Lithuania.
authors.forename: Irena
authors.identifier: 0000-0001-7422-2333
authors.initials: I
authors.lastname: Dumalakiene
mesh_terms: D000906:Antibodies / Q000276:immunology; D016022:Case-Control Studies; D002465:Cell Movement / Q000276:immunology; D005260:Female; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012859:Sjogren's Syndrome / Q000276:immunology*
chemical_list: D000906:Antibodies