title: Defining the phenotype of neutrophils following reverse migration in zebrafish.
abstract: Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.
authors.affiliation: Bateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Felix
authors.initials: F
authors.lastname: Ellett
authors.affiliation: Bateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Elks
authors.affiliation: Bateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Anne L
authors.initials: AL
authors.lastname: Robertson
authors.affiliation: Bateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Ogryzko
authors.affiliation: Bateson Centre and Department of Infection and Immunity, University of Sheffield, Sheffield, United Kingdom s.a.renshaw@sheffield.ac.uk.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Renshaw
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology; D015027:Zebrafish / Q000235:genetics / Q000276:immunology*
keywords: chemotaxis; granulocytes; infection; inflammation; phagocytosis