title: Determination of Caudatin in Rat Plasma by UPLC-MS/MS: Application to a Preclinical Pharmacokinetic Study.
abstract: In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of caudatin in rat plasma using carbamazepine as internal standard (IS). This method was linear over the concentration range 2.5-300 ng/ml with a lower limit of quantification (LLOQ) of 2.5 ng/ml. Inter- and intra-day precision (RSD%) were all within 10% and the accuracy (RE%) was equal or lower than 5%. Recoveries of caudatin and IS were more than 80% and matrix effects were not significant. Stability studies showed that caudatin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of caudatin to rats.
authors.affiliation: Department of Anaesthesiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shan
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wu
authors.forename: Baiping
authors.initials: B
authors.lastname: Mao
authors.forename: Ren-Shan
authors.initials: RS
authors.lastname: Ge
mesh_terms: D000818:Animals; D002220:Carbamazepine / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006027:Glycosides / Q000097:blood* / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D013256:Steroids / Q000097:blood* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry
chemical_list: D006027:Glycosides; D013256:Steroids; C570806:caudatin; D002220:Carbamazepine