title: Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro.
abstract: It has been proved that terminally differentiated mature adipocytes possess abilities to dedifferentiate into fibroblast-like progeny cells with self-renewal and multiple differentiation, termed dedifferentiated fat (DFAT) cells. However, the biological properties of DFAT cells during long-term culture in vitro have not been elucidated. Here, we obtained fibroblast-like morphology of porcine DFAT cells by ceiling culture. During the dedifferentiation process, round mature adipocytes with single large lipid droplets changed into spindle-shaped cells accompanied by the adipogenic markers PPARγ, aP2, LPL, and Adiponectin significant downregulation. Flow cytometric analysis showed that porcine DFAT cells displayed similar cell-surface antigen profile to mesenchymal stem cells (MSCs). Furthermore, different passages of porcine DFAT cells during long-term culture in vitro retained high levels of cell viabilities (>97%), efficient proliferative capacity including population doubling time ranged from 20 h to 22 h and population doubling reached 47.40 ± 1.64 by 58 days of culture. In addition, porcine DFAT cells maintained the multiple differentiation capabilities into adipocytes, osteoblasts, and skeletal myocytes and displayed normal chromosomal karyotypes for prolonged passaging. Therefore, porcine DFAT cells may be a novel model of stem cells for studying the functions of gene in the different biological events.
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China.
authors.forename: Tongxing
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Yuanfei
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China ; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China ; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D052242:Adiponectin / Q000096:biosynthesis; D000273:Adipose Tissue / Q000254:growth & development*; D000818:Animals; D054337:Cell Dedifferentiation / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D059630:Mesenchymal Stem Cells; D047495:PPAR gamma / Q000096:biosynthesis; D013552:Swine
chemical_list: D052242:Adiponectin; D047495:PPAR gamma