title: Solvent Properties of Water in Aqueous Solutions of Elastin-Like Polypeptide.
abstract: The phase-transition temperatures of an elastin-like polypeptide (ELP) with the (GVGVP)40 sequence and solvent dipolarity/polarizability, hydrogen-bond donor acidity, and hydrogen-bond acceptor basicity in its aqueous solutions were quantified in the absence and presence of different salts (Na2SO4, NaCl, NaClO4, and NaSCN) and various osmolytes (sucrose, sorbitol, trehalose, and trimethylamine N-oxide (TMAO)). All osmolytes decreased the ELP phase-transition temperature, whereas NaCl and Na2SO4 decreased, and NaSCN and NaClO4 increased it. The determined phase-transition temperatures may be described as a linear combination of the solvent's dipolarity/polarizability and hydrogen-bond donor acidity. The linear relationship established for the phase-transition temperature in the presence of salts differs quantitatively from that in the presence of osmolytes, in agreement with different (direct and indirect) mechanisms of the influence of salts and osmolytes on the ELP phase-transition temperature.
authors.affiliation: Analiza, Inc., Cleveland, OH 44114, USA. Luisa.ferreira@cleveland-diagnostics.com.
authors.forename: Luisa A
authors.initials: LA
authors.lastname: Ferreira
authors.affiliation: Department of Chemical and Biomedical Engineering, Cleveland State University, Cleveland, OH 44115, USA. jamejs@gmail.com.
authors.forename: James T
authors.initials: JT
authors.lastname: Cole
authors.affiliation: Department of Chemistry, Philipps University, Marburg D-35032, Germany. reichardt@chemie.uni-marburg.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Reichardt
authors.affiliation: Department of Chemical and Biomedical Engineering, Cleveland State University, Cleveland, OH 44115, USA. n.holland1@csuohio.edu.
authors.forename: Nolan B
authors.initials: NB
authors.lastname: Holland
authors.affiliation: Department of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. vuversky@health.usf.edu.|Institute for Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia. vuversky@health.usf.edu.|Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. vuversky@health.usf.edu.|Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia. vuversky@health.usf.edu.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Uversky
authors.affiliation: Analiza, Inc., Cleveland, OH 44114, USA. Boris.Zaslavsky@Cleveland-Diagnostics.com.
authors.forename: Boris Y
authors.initials: BY
authors.lastname: Zaslavsky
mesh_terms: D004187:Disaccharides / Q000737:chemistry; D004549:Elastin / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D044366:Transition Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D010455:Peptides; D012492:Salts; D012997:Solvents; D014867:Water; D004549:Elastin
keywords: and hydrogen-bond acceptor basicity; elastin-like polypeptide; hydrogen-bond donor acidity; osmolyte; phase-transition temperature; solvent dipolarity/polarizability; solvent properties