title: Animal ice-binding (antifreeze) proteins and glycolipids: an overview with emphasis on physiological function.
abstract: Ice-binding proteins (IBPs) assist in subzero tolerance of multiple cold-tolerant organisms: animals, plants, fungi, bacteria etc. IBPs include: (1) antifreeze proteins (AFPs) with high thermal hysteresis antifreeze activity; (2) low thermal hysteresis IBPs; and (3) ice-nucleating proteins (INPs). Several structurally different IBPs have evolved, even within related taxa. Proteins that produce thermal hysteresis inhibit freezing by a non-colligative mechanism, whereby they adsorb onto ice crystals or ice-nucleating surfaces and prevent further growth. This lowers the so-called hysteretic freezing point below the normal equilibrium freezing/melting point, producing a difference between the two, termed thermal hysteresis. True AFPs with high thermal hysteresis are found in freeze-avoiding animals (those that must prevent freezing, as they die if frozen) especially marine fish, insects and other terrestrial arthropods where they function to prevent freezing at temperatures below those commonly experienced by the organism. Low thermal hysteresis IBPs are found in freeze-tolerant organisms (those able to survive extracellular freezing), and function to inhibit recrystallization - a potentially damaging process whereby larger ice crystals grow at the expense of smaller ones - and in some cases, prevent lethal propagation of extracellular ice into the cytoplasm. Ice-nucleator proteins inhibit supercooling and induce freezing in the extracellular fluid at high subzero temperatures in many freeze-tolerant species, thereby allowing them to control the location and temperature of ice nucleation, and the rate of ice growth. Numerous nuances to these functions have evolved. Antifreeze glycolipids with significant thermal hysteresis activity were recently identified in insects, frogs and plants.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA Duman.1@nd.edu.
authors.forename: John G
authors.initials: JG
authors.lastname: Duman
mesh_terms: D000064:Acclimatization; D000818:Animals; D021301:Antifreeze Proteins / Q000378:metabolism*; D003081:Cold Climate; D003451:Cryoprotective Agents / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism*; D007053:Ice
chemical_list: D021301:Antifreeze Proteins; D003451:Cryoprotective Agents; D006017:Glycolipids; D007053:Ice
keywords: Antifreeze proteins; Cold tolerance; Ice-binding proteins; Ice-nucleating proteins