title: In vitro digestibility of goat milk and kefir with a new standardised static digestion method (INFOGEST cost action) and bioactivities of the resultant peptides.
abstract: The hydrolysis degrees of goat milk and kefir during simulated gastrointestinal digestion and some bioactivities of the resulting peptides after fermentation and digestion were studied. A static in vitro digestion method by the COST FA1005 Action INFOGEST was used and goat milk and kefir were partially hydrolyzed during the gastric phase and had above 80% hydrolysis after duodenal digestion. There were no differences between the digestibility of goat milk and kefir (p > 0.05). Goat milk and kefir displayed about 7-fold antioxidant activity after digestion (p < 0.05). Fermentation showed no effect on the calcium-binding capacity of the samples (p > 0.05), however, after in vitro digestion calcium-binding capacity of the goat milk and kefir increased 2 and 5 fold, respectively (p < 0.05). Digested goat milk and kefir showed a higher dose-dependent inhibitory effect on α-amylase compared to undigested samples (p < 0.05). α-Glucosidase inhibitory activities and in vitro bile acid-binding capacities of the samples were not determined at the studied concentrations.
authors.affiliation: Department of Food Engineering, Nutrition Section, Engineering Faculty of Ege University, 35100, İzmir, Turkey. sedef.el@ege.edu.tr.
authors.forename: Sedef
authors.initials: S
authors.lastname: Nehir El
authors.forename: Sibel
authors.initials: S
authors.lastname: Karakaya
authors.forename: Sebnem
authors.initials: S
authors.lastname: Simsek
authors.forename: Didier
authors.initials: D
authors.lastname: Dupont
authors.forename: Esra
authors.initials: E
authors.lastname: Menfaatli
authors.forename: Alper Tolga
authors.initials: AT
authors.lastname: Eker
mesh_terms: D000818:Animals; D043302:Cultured Milk Products / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D004063:Digestion; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000378:metabolism*; D006041:Goats; D006801:Humans; D006868:Hydrolysis; D056546:Lactobacillales / Q000378:metabolism*; D008892:Milk / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D010455:Peptides; D000516:alpha-Amylases; D000520:alpha-Glucosidases