title: Maintenance of bladder urothelia integrity and successful urothelialization of various tissue-engineered mesenchymes in vitro.
abstract: Tissue-engineering offers the opportunity to produce hybrid tissues in vitro. The induction of bladder urothelial cells (BUCs) differentiation in vitro has been assessed by several research groups to build bladder models for fundamental studies and clinical applications. However, BUC induction of advanced differentiation in culture remains a challenging task. To reach this goal, optimal culture conditions are required, notably the use of specific additives as well as proper mesenchymal support. The best positive control for BUCs functional state monitoring is native urothelium collected from healthy bladder samples. In order to establish the best culture conditions to maintain and promote BUC differentiated state, native urothelia were cultured on various mesenchymes. Native bladder mesenchymes were used as controls for the maintenance of native urothelia. Histological and ultrastructural analyses showed the necessity to have a cellularized mesenchyme for rapid formation of a pseudostratified urothelium, allowing apical membrane rearrangement of the superficial cells in culture. Taken together, the results strongly suggest that it is possible to conserve the integrity of urothelia in vitro and, thus, potentially use them for eventual clinical applications and pharmacological investigations.
authors.affiliation: Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX Faculté de médecine, Université Laval, Québec, Canada. sara.ucergy@hotmail.fr.|Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Faculté de médecine, Centre de Recherche du CHU de Québec, Université Laval, Québec, Canada. sara.ucergy@hotmail.fr.
authors.forename: Sara
authors.initials: S
authors.lastname: Bouhout
authors.affiliation: Service d'anatomopathologie du CHU de Québec, Québec, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Tremblay
authors.affiliation: Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Faculté de médecine, Centre de Recherche du CHU de Québec, Université Laval, Québec, Canada.
authors.forename: Stephane
authors.initials: S
authors.lastname: Bolduc
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D008648:Mesoderm / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009130:Muscle, Smooth / Q000166:cytology; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D001743:Urinary Bladder / Q000166:cytology*; D019459:Urothelium / Q000166:cytology*
chemical_list: D003470:Culture Media
keywords: Bladder hybrid constructs; Collagen matrix; Tissue-engineered urothelia