title: Resveratrol increases glycolytic flux in Saccharomyces cerevisiae via a SNF1-dependet mechanism.
abstract: Evidence suggests that AMP protein kinase (AMPK) is the main target of the phytochemical resveratrol (RSV) in mammalian cells. Data also indicates that RSV stimulates glucose metabolism; however, the molecular link between RSV and glucose uptake remains unknown. Herein, we provide evidence indicating that RSV stimulates glycolysis via sucrose non-fermenting 1 gene (SNF1, Saccharomyces cerevisiae orthologous of AMPK). S. cerevisiae cultures treated with 30 μM RSV showed an increase in extracellular acidification rate compared to untreated cells, indicating an elevated glycolytic flux. Also, RSV treatment increased transcription levels of two key glycolytic genes, hexokinase 2 (HXK2) and phosphofructokinase 1 (PFK1), as well as production of NADH. Moreover, RSV treatment inhibited mitochondrial respiration when glucose was used as a carbon source. Importantly, the effects of RSV on glycolysis were dependent of SNF1. Taken together, these findings suggest that SNF1 (AMPK in mammalian systems) is the molecular target of RSV in S. cerevisiae.
authors.affiliation: Laboratorio de Biotecnología Microbiana del Instituto Tecnológico Superior de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez #2120, Ciudad Hidalgo, Michoacán, 61100, México.
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Madrigal-Perez
authors.forename: Gerardo M
authors.initials: GM
authors.lastname: Nava
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: González-Hernández
authors.forename: Minerva
authors.initials: M
authors.lastname: Ramos-Gomez
mesh_terms: D006019:Glycolysis / Q000187:drug effects*; D006593:Hexokinase / Q000096:biosynthesis / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010732:Phosphofructokinase-1 / Q000096:biosynthesis / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics; D013267:Stilbenes / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D013267:Stilbenes; C071570:SNF1-related protein kinases; C490214:HXK2 protein, S cerevisiae; D006593:Hexokinase; D010732:Phosphofructokinase-1; D017346:Protein Serine-Threonine Kinases; D000077185:Resveratrol