title: Mass Spectrometry in Pharmacokinetic Studies of a Synthetic Compound for Spinal Cord Injury Treatment.
abstract: The studies of drugs that could constitute a palliative to spinal cord injury (SCI) are a continuous and increasing demand in biomedicine field from developed societies. Recently we described the chemical synthesis and antiglioma activity of synthetic glycosides. A synthetic sulfated glycolipid (here IG20) has shown chemical stability, solubility in polar solvents, and high inhibitory capacity over glioma growth. We have used mass spectrometry (MS) to monitor IG20 (m/z = 550.3) in cells and tissues of the central nervous system (CNS) that are involved in SCI recovery. IG20 was detected by MS in serum and homogenates from CNS tissue of rats, though in the latter a previous deproteinization step was required. The pharmacokinetic parameters of serum clearance at 24 h and half-life at 4 h were determined for synthetic glycoside in the adult rat using MS. A local administration of the drug near of spinal lesion site is proposed.
authors.affiliation: Grupo de Química Neuro-Regenerativa, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La-Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain.
authors.forename: María
authors.identifier: 0000-0001-5524-2443
authors.initials: M
authors.lastname: Sánchez-Sierra
authors.affiliation: Grupo de Química Neuro-Regenerativa, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La-Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain.
authors.forename: Isabel
authors.identifier: 0000-0002-2273-5046
authors.initials: I
authors.lastname: García-Álvarez
authors.affiliation: Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Alfonso
authors.identifier: 0000-0002-1241-7538
authors.initials: A
authors.lastname: Fernández-Mayoralas
authors.affiliation: Grupo de Química Neuro-Regenerativa, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La-Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Moreno-Lillo
authors.affiliation: Servicio de Proteómica, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La-Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain.
authors.forename: Gemma
authors.identifier: 0000-0003-3200-8659
authors.initials: G
authors.lastname: Barroso García
authors.affiliation: Servicio de Proteómica, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La-Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain.
authors.forename: Verónica
authors.identifier: 0000-0001-6425-1851
authors.initials: V
authors.lastname: Moral Dardé
authors.affiliation: Grupo de Química Neuro-Regenerativa, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La-Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain.
authors.forename: Ernesto
authors.identifier: 0000-0002-2704-9782
authors.initials: E
authors.lastname: Doncel-Pérez
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D006017:Glycolipids / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000097:blood* / Q000188:drug therapy* / Q000473:pathology
chemical_list: D006017:Glycolipids; C011117:sulfoglycolipids