title: Site-Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS).
abstract: A method for comprehensive glycoprotein characterization via glycopeptide generation and analysis is presented. Parallel analysis of a deglycosylated sample and the use of buffered solvent simplify the identification of peaks comprising the glycopattern of a given peptide.
authors.affiliation: Department of Chemistry, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
authors.forename: Clemens
authors.initials: C
authors.lastname: Gruber
authors.forename: Friedrich
authors.initials: F
authors.lastname: Altmann
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D010446:Peptide Fragments / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D006023:Glycoproteins; D010446:Peptide Fragments