title: Statistical modeling using early markers of innate immunity to explain variation in humoral responses to influenza vaccine in older adults.
abstract: Greater understanding of the factors associated with a protective response to influenza vaccine in older adults could have tremendous public health benefits. We studied 158 participants age 50-74 years vaccinated with 2010-2011 inactivated influenza vaccine and performed innate immunity and humoral immunity assays directed against influenza A/California/2009 (H1N1) as measured through hemagglutination inhibition (HAI), microneutralization, and B cell ELISPOT at days 0, 3, and 28 postvaccination. We report the results of statistical modeling using Day 3 cytokines, chemokines, and innate cell populations to model Day 0 to Day 28 HAI seroconversion, viral neutralization seroconversion, and B cell ELISPOT results.
authors.affiliation: Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN, USA.
authors.forename: Richard B
authors.initials: RB
authors.lastname: Kennedy
authors.affiliation: Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN, USA; Division of Infectious Diseases, Mayo Clinic, Rochester, MN, USA.
authors.forename: Pritish K
authors.initials: PK
authors.lastname: Tosh
authors.affiliation: Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA.
authors.forename: Krista M
authors.initials: KM
authors.lastname: Goergen
authors.affiliation: Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA.
authors.forename: Diane E
authors.initials: DE
authors.lastname: Grill
authors.affiliation: Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA.
authors.forename: Ann L
authors.initials: AL
authors.lastname: Oberg
authors.affiliation: Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN, USA; Division of General Internal Medicine, Mayo Clinic, Rochester, MN, USA. Electronic address: Poland.gregory@mayo.edu.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Poland
mesh_terms: D000367:Age Factors; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood*; D015415:Biomarkers / Q000032:analysis*; D002140:California; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007113:Immunity, Innate; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D009500:Neutralization Tests; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015415:Biomarkers; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated
keywords: Adult; Age factors; Aged; Immunity, Humoral; Influenza vaccines; Models, Statistical