title: Short Communication: Virion Aggregation by Neutralizing and Nonneutralizing Antibodies to the HIV-1 Envelope Glycoprotein.
abstract: We used dynamic light scattering to detect aggregation of HIV-1 virions by antibodies (IgG) to the viral envelope glycoprotein (Env). Virions of different strains were inactivated by 2,2'-dithiodipyridine (AT-2), a procedure that abrogates infectivity but preserves the native antigenic structure of Env. Neutralizing antibodies directed to a V3-base- and glycan-dependent epitope on gp120 and to the apex of the Env trimer, as well as nonneutralizing antibodies to the epitope cluster I on the gp41-ectodomain, aggregated virions, but in markedly narrow concentration ranges. In contrast, the neutralizing antibody 2G12, which is specific for a composite glycan-dependent epitope on gp120 and functionally monovalent because of its unusual domain-swap structure, was nonaggregating. These results have potentially complex implications for vaccine development.
authors.affiliation: 1 Department of Microbiology and Immunology, Weill Cornell Medical College , Cornell University, New York, New York.
authors.forename: Marina R
authors.initials: MR
authors.lastname: Alexander
authors.affiliation: 1 Department of Microbiology and Immunology, Weill Cornell Medical College , Cornell University, New York, New York.|2 Department of Medical Microbiology, Academic Medical Center, University of Amsterdam , Amsterdam, the Netherlands .
authors.forename: Rogier W
authors.initials: RW
authors.lastname: Sanders
authors.affiliation: 1 Department of Microbiology and Immunology, Weill Cornell Medical College , Cornell University, New York, New York.
authors.forename: John P
authors.initials: JP
authors.lastname: Moore
authors.affiliation: 1 Department of Microbiology and Immunology, Weill Cornell Medical College , Cornell University, New York, New York.
authors.forename: Per Johan
authors.initials: PJ
authors.lastname: Klasse
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology; D000067493:Dynamic Light Scattering; D006023:Glycoproteins; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D014771:Virion / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D006023:Glycoproteins; D015483:HIV Antibodies; D007074:Immunoglobulin G; D054299:env Gene Products, Human Immunodeficiency Virus