title: The amino acid 419 in HC-Pro is involved in the ability of PVY isolate N605 to induce necrotic symptoms on potato tubers.
abstract: The ability to induce the potato tuber necrosis ringspot disease (PTNRD) is a property shared by PVY isolates belonging to different groups (e.g. PVY(N) and PVY(O)) and variants (e.g. PVY(NTN) and PVY(N)-W). The identification of viral molecular determinant(s) involved in the expression of PTNRD symptoms is essential for (i) an easier detection of tuber necrosis isolates and (ii) an improvement of our knowledge on the epidemiology of this potato disease. A reverse genetic approach associated with a biological typing of a collection of PVY chimeras and mutants indicated that residue E419 of the HC-Pro protein is linked to the ability of PVY to induce tuber necrosis on four PTNRD-susceptible potato cultivars. Indeed, the substitution of the N-type glutamic acid (E) in O-type aspartic acid (D) at position 419 in the HC-Pro cistron prevents the expression of tuber necrosis on infected tubers without reducing the virulence of the corresponding E/D419 mutant. This result opens opportunities for the future studies on potato/PVY interactions.
authors.affiliation: FN3PT/RD3PT, 43-45 rue de Naples, F-75008 Paris, France; INRA, UMR 1349 IGEPP, F-35653 Le Rheu, France. Electronic address: laurent.glais@fnpppt.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Glais
authors.affiliation: FN3PT/RD3PT, 43-45 rue de Naples, F-75008 Paris, France; INRA, UMR 1349 IGEPP, F-35653 Le Rheu, France. Electronic address: florence.faurez@inserm.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Faurez
authors.affiliation: INRA, UMR 1349 IGEPP, F-35653 Le Rheu, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Tribodet
authors.affiliation: FN3PT/RD3PT, 43-45 rue de Naples, F-75008 Paris, France; INRA, UMR 1349 IGEPP, F-35653 Le Rheu, France. Electronic address: frederic.boulard@fnpppt.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Boulard
authors.affiliation: INRA-Cirad-Supagro Montpellier, UMR 385 BGPI, F-34398 Montpellier, France. Electronic address: emmanuel.jacquot@supagro.inra.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Jacquot
mesh_terms: D020816:Amino Acid Motifs; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology*; D035281:Plant Tubers / Q000821:virology*; D017800:Potyvirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D011198:Solanum tuberosum / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus
keywords: HC-Pro; Infectious clone; PTNRD; PVY(NTN); Potato