title: Spatial distribution of Brucella antibodies with reference to indigenous cattle populations among contrasting agro-ecological zones of Uganda.
abstract: Indigenous cattle populations exhibit various degrees of agro-ecological fitness and provide desirable opportunities for investments to improve sustainable production for better rural small-scale farmers' incomes globally. However, they could be a source of infection to their attendants and other susceptible livestock if their brucellosis status remains unknown. This study investigated the spatial distribution of Brucella antibodies among indigenous cattle populations in Uganda. Sera from a total of 925 indigenous cattle (410 Ankole Bos taurus indicus, 50 Nganda and 465 East African Shorthorn Zebu (EASZ) - B. indicus) obtained randomly from 209 herds spread throughout Uganda were sequentially analysed for Brucella antibodies using the indirect (I) and competitive (C) enzyme linked Immuno-sorbent assays (ELISA). Recent incidences of abortion within the previous 12 months and routine hygienic practices during parturition were explored for public health risks. Brucella antibodies occurred in approximately 8.64% (80/925) and 28.70% (95% CI: 22.52, 34.89) of the sampled individual cattle and herds, respectively. Findings have shown that Ankole and EASZ cattle had similar seroprevalences. Indigenous cattle from the different study agro-ecological zones (AEZs) exhibited varying seroprevalences ranging from approximately 1.78% (95% CI: 0, 5.29) to 19.67% (95% CI: 8.99, 30.35) in the Lake Victoria Crescent (LVC) and North Eastern Drylands (NED) respectively. Significantly higher odds for Brucella antibodies occurred in the NED (OR: 3.40, 95% CI: 1.34, 8.57, p=0.01) inhabited by EASZ cattle compared to the KP (reference category) AEZ. Recent incidences of abortions within the previous 12 months were significantly (p<0.001) associated with seropositive herds. These findings add critical evidence to existing information on the widespread occurrence of brucellosis among indigenous cattle populations in Uganda and could guide allocation of meagre resources for awareness creation. And deployment of control strategies including culling of older cattle and those which have aborted during advanced gestation, enforcement of hygiene practices and mass vaccination.
authors.affiliation: Department of Environmental Management, Molecular Genetics Laboratory, College of Agricultural and Environmental Sciences, Makerere University, P. O. Box 7062/7298, Kampala, Uganda; National Livestock Resources Research Institute (NaLIRRI), P.O. Box 96, Tororo, Uganda. Electronic address: freddykabi@gmail.com.
authors.forename: Fredrick
authors.initials: F
authors.lastname: Kabi
authors.affiliation: Department of Environmental Management, Molecular Genetics Laboratory, College of Agricultural and Environmental Sciences, Makerere University, P. O. Box 7062/7298, Kampala, Uganda.
authors.forename: Vincent
authors.initials: V
authors.lastname: Muwanika
authors.affiliation: Department of Bio-Sciences, College of Natural Sciences, Makerere University, Box 7062, Kampala, Uganda.
authors.forename: Charles
authors.initials: C
authors.lastname: Masembe
mesh_terms: D000034:Abortion, Veterinary / Q000453:epidemiology / Q000382:microbiology; D000822:Animal Husbandry; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002002:Brucella / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D002417:Cattle; D017753:Ecosystem; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D015994:Incidence; D008297:Male; D015995:Prevalence; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D014454:Uganda / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial
keywords: Agro-ecological zones; Brucella antibodies; Indigenous cattle populations; Spatial distribution; Uganda