title: A generic methodological framework for studying single cell motility in high-throughput time-lapse data.
abstract: BACKGROUND
  Motility is a fundamental cellular attribute, which plays a major part in processes ranging from embryonic development to metastasis. Traditionally, single cell motility is often studied by live cell imaging. Yet, such studies were so far limited to low throughput. To systematically study cell motility at a large scale, we need robust methods to quantify cell trajectories in live cell imaging data.
  
  
  RESULTS
  The primary contribution of this article is to present Motility study Integrated Workflow (MotIW), a generic workflow for the study of single cell motility in high-throughput time-lapse screening data. It is composed of cell tracking, cell trajectory mapping to an original feature space and hit detection according to a new statistical procedure. We show that this workflow is scalable and demonstrates its power by application to simulated data, as well as large-scale live cell imaging data. This application enables the identification of an ontology of cell motility patterns in a fully unsupervised manner.
  
  
  METHODS
  Python code and examples are available online (http://cbio.ensmp.fr/∼aschoenauer/motiw.html)
authors.affiliation: MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Schoenauer Sebag
authors.affiliation: MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Plancade
authors.affiliation: MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Raulet-Tomkiewicz
authors.affiliation: MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Barouki
authors.affiliation: MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Vert
authors.affiliation: MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Walter
mesh_terms: D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D059010:Single-Cell Analysis; D012984:Software; D059008:Time-Lapse Imaging / Q000379:methods*; D057188:Workflow