title: Resistance to Cucurbit aphid-borne yellows virus in Melon Accession TGR-1551.
abstract: The genetic control of resistance to Cucurbit aphid-borne yellows virus (CABYV; genus Polerovirus, family Luteoviridae) in the TGR-1551 melon accession was studied through agroinoculation of a genetic family obtained from the cross between this accession and the susceptible Spanish cultivar 'Bola de Oro'. Segregation analyses were consistent with the hypothesis that one dominant gene and at least two more modifier genes confer resistance; one of these additional genes is likely present in the susceptible parent 'Bola de Oro'. Local and systemic accumulation of the virus was analyzed in a time course experiment, showing that TGR-1551 resistance was expressed systemically as a significant reduction of virus accumulation compared with susceptible controls, but not locally in agroinoculated cotyledons. In aphid transmission experiments, CABYV inoculation by aphids was significantly reduced in TGR-1551 plants, although the virus was acquired at a similar rate from TGR-1551 as from susceptible plants. Results of feeding behavior studies using the DC electrical penetration graph technique suggested that viruliferous aphids can salivate and feed from the phloem of TGR-1551 plants and that the observed reduction in virus transmission efficiency is not related to reduced salivation by Aphis gossypii in phloem sieve elements. Since the virus is able to accumulate to normal levels in agroinoculated tissues, our results suggest that resistance of TGR-1551 plants to CABYV is related to impairment of virus movement or translocation after it reaches the phloem sieve elements.
authors.affiliation: First, second, and sixth authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; third and fourth authors: Instituto de Ciencias Agrarias (ICA), CSIC, Serrano 115 dpdo, 28006, Madrid, Spain; and fifth author: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Mona A
authors.initials: MA
authors.lastname: Kassem
authors.affiliation: First, second, and sixth authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; third and fourth authors: Instituto de Ciencias Agrarias (ICA), CSIC, Serrano 115 dpdo, 28006, Madrid, Spain; and fifth author: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Gosalvez
authors.affiliation: First, second, and sixth authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; third and fourth authors: Instituto de Ciencias Agrarias (ICA), CSIC, Serrano 115 dpdo, 28006, Madrid, Spain; and fifth author: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Garzo
authors.affiliation: First, second, and sixth authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; third and fourth authors: Instituto de Ciencias Agrarias (ICA), CSIC, Serrano 115 dpdo, 28006, Madrid, Spain; and fifth author: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fereres
authors.affiliation: First, second, and sixth authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; third and fourth authors: Instituto de Ciencias Agrarias (ICA), CSIC, Serrano 115 dpdo, 28006, Madrid, Spain; and fifth author: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Gómez-Guillamón
authors.affiliation: First, second, and sixth authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; third and fourth authors: Instituto de Ciencias Agrarias (ICA), CSIC, Serrano 115 dpdo, 28006, Madrid, Spain; and fifth author: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Aranda
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D018548:Cotyledon / Q000821:virology; D028463:Cucumis melo / Q000821:virology; D019662:Cucurbitaceae / Q000821:virology*; D005247:Feeding Behavior; D052679:Luteoviridae / Q000502:physiology*; D052585:Phloem / Q000382:microbiology; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000469:parasitology
keywords: Cucumis melo; luteovirus