title: Benefits from additives and xylanase during enzymatic hydrolysis of bamboo shoot and mature bamboo.
abstract: Effects of additives (BSA, PEG 6000, and Tween 80) on enzymatic hydrolysis of bamboo shoot and mature bamboo fractions (bamboo green, bamboo timber, bamboo yellow, bamboo node, and bamboo branches) by cellulases and/or xylanase were evaluated. The addition of additives was comparable to the increase of cellulase loadings in the conversion of cellulose and xylan in bamboo fractions. Supplementation of xylanase (1 mg/g DM) with cellulases (10 FPU/g DM) in the hydrolysis of bamboo fractions was more efficient than addition of additives in the production of glucose and xylose. Moreover, addition of additives could further increase the glucose release from different bamboo fractions by cellulases and xylanase. Bamboo green exhibited the lowest hydrolyzability. Almost all of the polysaccharides in pretreated bamboo shoot fractions were hydrolyzed by cellulases with the addition of additives or xylanase. Additives and xylanase showed great potential for reducing cellulase requirement in the hydrolysis of bamboo.
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
authors.forename: Kena
authors.initials: K
authors.lastname: Li
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China. Electronic address: junhuazhang@nwsuaf.edu.cn.
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D031787:Sasa / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D011136:Polysorbates; D012710:Serum Albumin, Bovine; C000595215:Polyethylene Glycol 6000; D011092:Polyethylene Glycols; D000641:Ammonia; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: Additives; Bamboo shoot; Cellulases; Mature bamboo; Xylanase