title: Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.
abstract: BACKGROUND
  There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People's Republic of China.
  
  
  RESULTS
  The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments.
  
  
  CONCLUSIONS
  DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China.
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China. ynfy428@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China. shihongfu@hotail.com.
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Fu
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China. yangwh0604@163.com.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Yang
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China. second7368@163.com.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin, 130062, People's Republic of China. heb-001001@163.com.
authors.forename: Biao
authors.initials: B
authors.lastname: He
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin, 130062, People's Republic of China. changchun_tu@hotmail.com.
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Tu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China. gdliang@hotmail.com.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China. zhangHL715@163.com.
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D003465:Culex / Q000821:virology*; D016679:Genome, Viral; D016851:Orthobunyavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins