title: Bricks and blueprints: methods and standards for DNA assembly.
abstract: DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.
authors.affiliation: Centre for Synthetic Biology and Innovation, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Arturo
authors.initials: A
authors.lastname: Casini
authors.affiliation: Centre for Synthetic Biology and Innovation, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Marko
authors.initials: M
authors.lastname: Storch
authors.affiliation: Centre for Synthetic Biology and Innovation, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Geoffrey S
authors.initials: GS
authors.lastname: Baldwin
authors.affiliation: Centre for Synthetic Biology and Innovation, Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Ellis
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004720:Endonucleases / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods / Q000592:standards; D011995:Recombination, Genetic; D012015:Reference Standards; D058615:Synthetic Biology
chemical_list: D004720:Endonucleases