title: Inhibitory effect of various breads on DMH-induced aberrant crypt foci and colorectal tumours in rats.
abstract: Bread is rich in dietary fibre and many phytochemical compounds, which may influence chemoprevention of colon cancer. In the present study, we evaluated the effect of three kinds of bread on DMH-induced colorectal tumours in F344 rats. F344 rats were divided into four groups (Steinmetz Three-Grain bread, Steinmetz Country bread, White bread, and MF). All groups were injected with 1,2-dimethylhydrazine (DMH, 20 mg/kg body weight) once a week for 8 consecutive weeks from 5 weeks of age. To investigate the antioxidant effect of bread, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging rate of bread and the serum levels of 8-hydroxy-deoxyguanosine (8-OHdG) in rats were examined. The number of colorectal aberrant crypt foci (ACF) and the incidence of colorectal tumours were studied after 34 weeks of DMH treatment. The Steinmetz Three-Grain and Steinmetz Country bread groups had higher scavenging rates of the DPPH free radical and lower serum levels of 8-OHdG and incidence of ACF, adenomas, and adenocarcinomas of colon than the White bread and MF group. Steinmetz Three-Grain bread and Steinmetz Country bread have various ingredient combinations that may inhibit colorectal cancer progression.
authors.affiliation: Department of Pathology (Affiliated Hospital), Physiopathology and Academic Administration, Guilin Medical University, Guilin, Guangxi 541004, China ; Department of Health Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan.
authors.forename: Guangying
authors.initials: G
authors.lastname: Qi
authors.affiliation: Department of Pathology (Affiliated Hospital), Physiopathology and Academic Administration, Guilin Medical University, Guilin, Guangxi 541004, China.
authors.forename: Sien
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Health Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan.
authors.forename: Tiri
authors.initials: T
authors.lastname: Takashima
authors.affiliation: Department of Health Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Nozoe
authors.affiliation: Research and Development of Baking, Andersen Institute of Bread & Life Co., Ltd., Hiroshima 739-0323, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Shobayashi
authors.affiliation: Department of Food Sciences and Biotechnology, Hiroshima Institute of Technology, Hiroshima 731-5193, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kakugawa
authors.affiliation: Department of Food Sciences and Biotechnology, Hiroshima Institute of Technology, Hiroshima 731-5193, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Murakami
authors.affiliation: Department of Health Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Jikihara
authors.affiliation: Department of Pathology (Affiliated Hospital), Physiopathology and Academic Administration, Guilin Medical University, Guilin, Guangxi 541004, China ; Department of Health Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Health Sciences, Prefectural University of Hiroshima, Minami-ku, Hiroshima 734-8558, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimamoto
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity*; D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001939:Bread; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000975:Antioxidants; D019813:1,2-Dimethylhydrazine