title: Associations between the properties of the cartilage matrix and findings from quantitative MRI in human osteoarthritic cartilage of the knee.
abstract: The aim of this study was to investigate the associations between the properties of the cartilage matrix and the results of T2 mapping and delayed gadolinium-enhanced magnetic resonance imaging (dGEMRIC) in human knee osteoarthritic cartilage. Osteochondral samples were harvested from the middle part of the femoral condyle and tibial plateaus of 20 patients with knee osteoarthritis (OA) during total knee arthroplasty. Sagittal T2 mapping, T1pre, and T1Gd were performed using 7.0T magnetic resonance imaging (MRI). Glycosaminoglycan (GAG) distribution was evaluated by OARSI, collagen anisotropy was assessed by polarized light microscopy (PLM), and biochemical analyses measured water, GAG, and collagen content. Associations between properties of the cartilage matrix and T2 and ΔR1 (1/T1Gd-1/T1pre) values were explored using correlation analysis. T2 and ΔR1 values were significantly correlated with the degree of cartilage degeneration (OARSI grade; Ρ = 0.53 and 0.77). T2 values were significantly correlated with water content (r = 0.69; P < 0.001), GAG content (r = -0.43; P < 0.001), and PLM grade (r = 0.47; P < 0.001), but not with collagen content (r = -0.02; P = 0.110). ΔR1 values were significantly correlated with GAG content (r = -0.84; P < 0.001) and PLM grade (r = 0.41; P < 0.001). Taken together, T2 mapping and dGEMRIC results were correlated with the properties of the cartilage matrix in human knee osteoarthritic cartilage. Combination T2 mapping and dGEMRIC represents a potential non-invasive monitoring technique to detect the progress of knee OA.
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China ; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China ; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Radiology, The First Affiliated Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China ; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Fengyong
authors.initials: F
authors.lastname: Mao
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China ; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China ; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Radiology, Jiangsu Key Laboratory of Molecule Imaging and Functional Imaging, Zhong Da Hospital, Medical School of Southeast University Nanjing, Jiangsu, China.
authors.forename: Fengchao
authors.initials: F
authors.lastname: Zang
authors.affiliation: Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China ; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University Nanjing, Jiangsu, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D005260:Female; D006801:Humans; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D020370:Osteoarthritis, Knee / Q000378:metabolism / Q000473:pathology*
chemical_list: D003094:Collagen
keywords: 7.0T; T2 mapping; cartilage matrix; dGEMRIC; human osteoarthritic cartilage