title: Deliveries from trophectoderm biopsied, fresh and vitrified blastocysts derived from polar body biopsied, vitrified oocytes.
abstract: This longitudinal study reports preliminary findings of six patients who underwent first polar body biopsy followed by oocyte vitrification. All oocytes were warmed, inseminated by intracytoplasmic sperm injection and cultured to blastocyst. All suitable blastocysts underwent trophectoderm biopsy for aneuploidy screening, and supernumerary blastocysts were vitrified. Euploid blastocysts were transferred either fresh or in a subsequent programmed cycle. Of the 91 metaphase II oocytes, 30 had euploid first polar bodies. Development to blastocyst was more likely in oocytes with a euploid first polar body (66.7% versus 24.6%; P < 0.001). Nineteen euploid blastocysts were produced: 10 from oocytes with a euploid first polar body and nine from oocytes with an aneuploid first polar body. Five out of six patients (83%) had a live birth or ongoing pregnancy at the time of analysis. Eleven euploid blastocysts have been transferred and seven implanted (64%). Although the chromosomal status of the first polar body was poorly predictive of embryonic ploidy, an association was found between chromosomal status of the first polar body and development to blastocyst. Further study is required to characterize these relationships, but proof of concept is provided that twice biopsied, twice cryopreserved oocytes and embryos can lead to viable pregnancies.
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Jamie
authors.initials: J
authors.lastname: Grifo
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Alexis
authors.initials: A
authors.lastname: Adler
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Hsiao Ling
authors.initials: HL
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, New York University Langone Medical Center, 530 1st Ave. NBV 9E2, New York, NY 10016, USA. Electronic address: scottjmorin@gmail.com.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Morin
authors.affiliation: Department of Obstetrics and Gynecology, New York University Langone Medical Center, 530 1st Ave. NBV 9E2, New York, NY 10016, USA.
authors.forename: Meghan
authors.initials: M
authors.lastname: Smith
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Lucy
authors.initials: L
authors.lastname: Lu
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Brooke
authors.initials: B
authors.lastname: Hodes-Wertz
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Caroline
authors.initials: C
authors.lastname: McCaffrey
authors.affiliation: New York University Langone Medical Center Fertility Center, 660 1st Ave. 5th Floor, New York, NY 10016, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Berkeley
authors.affiliation: Reprogenetics, 3 Regent St. Livingston, NJ 07039, USA.
authors.forename: Santiago
authors.initials: S
authors.lastname: Munné
mesh_terms: D001755:Blastocyst / Q000166:cytology*; D004475:Ectoderm / Q000166:cytology; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D014327:Trophoblasts / Q000166:cytology
keywords: array comparative genomic hybridization; cryopreservation; embryo; fertility preservation; polar body biopsy; preimplantation genetic screening