title: Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces.
abstract: We describe a general approach to designing two-dimensional (2D) protein arrays mediated by noncovalent protein-protein interfaces. Protein homo-oligomers are placed into one of the seventeen 2D layer groups, the degrees of freedom of the lattice are sampled to identify configurations with shape-complementary interacting surfaces, and the interaction energy is minimized using sequence design calculations. We used the method to design proteins that self-assemble into layer groups P 3 2 1, P 4 2(1) 2, and P 6. Projection maps of micrometer-scale arrays, assembled both in vitro and in vivo, are consistent with the design models and display the target layer group symmetry. Such programmable 2D protein lattices should enable new approaches to structure determination, sensing, and nanomaterial engineering.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. Institute for Protein Design, University of Washington, Seattle, WA 98195, USA. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
authors.forename: Shane
authors.initials: S
authors.lastname: Gonen
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: DiMaio
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. gonent@janelia.hhmi.org dabaker@uw.edu.
authors.forename: Tamir
authors.initials: T
authors.lastname: Gonen
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. Institute for Protein Design, University of Washington, Seattle, WA 98195, USA. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA. gonent@janelia.hhmi.org dabaker@uw.edu.
authors.forename: David
authors.initials: D
authors.lastname: Baker
mesh_terms: D017076:Computer-Aided Design; D020285:Cryoelectron Microscopy; D040081:Protein Array Analysis; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins