title: In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates towards Osteoblastic Cells (hFOB1.19).
abstract: Casein and soy protein were digested by papain to three degrees of hydrolysis (DH) 7.3%-13.3%, to obtain respective six casein and soy protein hydrolysates, aiming to clarify their in vitro proliferation and anti-apoptosis towards a human osteoblastic cell line (hFOB1.19 cells). Six casein and soy protein hydrolysates at five levels (0.01-0.2 mg/mL) mostly showed proliferation as positive 17β-estradiol did, because they conferred the osteoblasts with cell viability of 100%-114% and 104%-123%, respectively. The hydrolysates of higher DH values had stronger proliferation. Casein and soy protein hydrolysates of the highest DH values altered cell cycle progression, and enhanced cell proportion of S-phase from 50.5% to 56.5% and 60.5%. The two also antagonized etoposide- and NaF-induced osteoblast apoptosis. In apoptotic prevention, apoptotic cells were decreased from 31.6% to 22.6% and 15.6% (etoposide treatment), or from 19.5% to 17.7% and 12.4% (NaF treatment), respectively. In apoptotic reversal, soy protein hydrolysate decreased apoptotic cells from 13.3% to 11.7% (etoposide treatment), or from 14.5% to 11.0% (NaF treatment), but casein hydrolysate showed no reversal effect. It is concluded that the hydrolysates of two kinds had estradiol-like action on the osteoblasts, and soy protein hydrolysates had stronger proliferation and anti-apoptosis on the osteoblasts than casein hydrolysates.
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. panxiaowen12358@126.com.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Pan
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. zhaoxh@neau.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, China. zhaoxh@neau.edu.cn.
authors.forename: Xin-Huai
authors.initials: XH
authors.lastname: Zhao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002364:Caseins / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005047:Etoposide / Q000494:pharmacology; D005333:Fetus / Q000187:drug effects / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology*; D010206:Papain / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology; D030262:Soybean Proteins / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002364:Caseins; D011492:Protein Hydrolysates; D030262:Soybean Proteins; C475729:casein hydrolysate; D005047:Etoposide; D012969:Sodium Fluoride; D010206:Papain
keywords: anti-apoptosis; casein hydrolysates; human fetal osteoblasts; proliferation; soy protein hydrolysates