title: Using infrared and Raman microspectroscopies to compare ex vivo involved psoriatic skin with normal human skin.
abstract: Psoriasis is a chronic dermatosis that affects around 3% of the world's population. The etiology of this autoimmune pathology is not completely understood. The barrier function of psoriatic skin is known to be strongly altered, but the structural modifications at the origin of this dysfunction are not clear. To develop strategies to reduce symptoms of psoriasis or adequate substitutes for modeling, a deep understanding of the organization of psoriatic skin at a molecular level is required. Infrared and Raman microspectroscopies have been used to obtain direct molecular-level information on psoriatic and healthy human skin biopsies. From the intensities and positions of specific vibrational bands, the lipid and protein distribution and the lipid order have been mapped in the different layers of the skin. Results showed a similar distribution of lipids and collagen for normal and psoriatic human skin. However, psoriatic skin is characterized by heterogeneity in lipid/protein composition at the micrometer scale, a reduction in the definition of skin layer boundaries and a decrease in lipid chain order in the stratum corneum as compared to normal skin. A global decrease of the structural organization is exhibited in psoriatic skin that is compatible with an alteration of its barrier properties.
authors.affiliation: Université Laval, Département de Génie des Mines, de la Métallurgie et des Matériaux, Laboratoire d'Ingénierie de Surface, Centre de Recherche sur les Matériaux Avancés, 1065 Avenue de la médecine, Québec G1V 0A6, CanadabCentre de Recherche du Centre Hosp.
authors.forename: Marie
authors.initials: M
authors.lastname: Leroy
authors.affiliation: Université Laval, Département de Chimie, Regroupement québécois sur la fonction, la structure et l'ingénierie des protéines, Centre de Recherche sur les Matériaux Avancés, 1045 Avenue de la médecine, Québec G1V 0A6, Canada.
authors.forename: Thierry
authors.initials: T
authors.lastname: Lefèvre
authors.affiliation: Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération, LOEX-CRCHU de Québec, Hôpital de l'Enfant Jésus, 1401, 18e rue, Québec G1J 1Z4, Canada.
authors.forename: Roxane
authors.initials: R
authors.lastname: Pouliot
authors.affiliation: Université Laval, Département de Chimie, Regroupement québécois sur la fonction, la structure et l'ingénierie des protéines, Centre de Recherche sur les Matériaux Avancés, 1045 Avenue de la médecine, Québec G1V 0A6, Canada.
authors.forename: Michèle
authors.initials: M
authors.lastname: Auger
authors.affiliation: Université Laval, Département de Génie des Mines, de la Métallurgie et des Matériaux, Laboratoire d'Ingénierie de Surface, Centre de Recherche sur les Matériaux Avancés, 1065 Avenue de la médecine, Québec G1V 0A6, CanadabCentre de Recherche du Centre Hosp.
authors.forename: Gaétan
authors.initials: G
authors.lastname: Laroche
mesh_terms: D000328:Adult; D000368:Aged; D003094:Collagen / Q000737:chemistry; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008055:Lipids / Q000737:chemistry; D008297:Male; D015591:Microspectrophotometry; D008875:Middle Aged; D011565:Psoriasis / Q000473:pathology*; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids; D003094:Collagen