title: Hsa-miR-132 inhibits proliferation of hepatic carcinoma cells by targeting YAP.
abstract: MicroRNAs and Yes-associated protein (YAP) play an important role in the occurrence and development of hepatic carcinomas. However, the interaction between microRNAs and YAP was seldom elucidated. In this study, we showed that miR-132 could target YAP gene by using dual-luciferase reporter system. Further quantitative polymerase chain reaction analysis and western blotting showed that miR-132 could significantly reduce the expression of YAP at mRNA and protein levels. Results of annexin V-fluorescein isothiocyanate, 5-ethynyl-2'-deoxyuridine staining and transwell assays showed that miR-132 significantly promoted the cell apoptosis and effectively inhibited the proliferation and invasion of hepatoma cells. These results indicated that miR-132 could inhibit the growth of hepatoma cells by targeting YAP gene and reducing its expression level. Taken together, results from this study would help us to understand the mechanisms for occurrence and development of hepatic carcinoma and provide new targets for diagnosis and treatment of liver cancer.
authors.affiliation: Department of General Surgery, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Chang-Jiang
authors.initials: CJ
authors.lastname: Lei
authors.affiliation: Department of General Surgery, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Oncology Department, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.affiliation: Laboratory Medicine, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Hanyang Affiliated Hospital of Wuhan University of Science & Technology, Wuhan, China.
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Pan
authors.affiliation: Department of General Surgery, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Hao-Cheng
authors.initials: HC
authors.lastname: Long
authors.affiliation: Department of General Surgery, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Chun-Zhou
authors.initials: CZ
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: De-Fa
authors.initials: DF
authors.lastname: Ren
authors.affiliation: Department of General Surgery, Fifth Hospital of Wuhan, Wuhan, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019295:Computational Biology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D010750:Phosphoproteins / Q000378:metabolism*; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C547077:MIRN132 microRNA, human; D035683:MicroRNAs; D010750:Phosphoproteins; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human
keywords: Hsa-miR-132; YAP; hepatic carcinoma cells; microRNA