title: Simple and efficient isolation of cordycepin from culture broth of a Cordyceps militaris mutant.
abstract: Isolation of cordycepin from the culture broth of Cordyceps militaris mutant was investigated. Based on the solubility curve, three crystallizing processes, temperature shift (process I), pH shift (process II), and pH shift followed by temperature shift (process III) were carried out. Process III was the most promising method regarding both purity and yield.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui-shi, Fukui 910-8507, Japan.
authors.forename: Mina
authors.initials: M
authors.lastname: Masuda
authors.affiliation: Research & Development Department, The Wakasa-wan Research Center, 64-52-1 Nagatani, Tsuruga-shi, Fukui 914-0192, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Hatashita
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui-shi, Fukui 910-8507, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Fujihara
authors.affiliation: Tenure-Track Program for Innovative Research, University of Fukui, 3-9-1 Bunkyo, Fukui-shi, Fukui 910-8507, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui-shi, Fukui 910-8507, Japan; Translational Research Center, Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address: a_sakura@u-fukui.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sakurai
mesh_terms: D032661:Cordyceps / Q000235:genetics* / Q000378:metabolism*; D003460:Crystallization; D003470:Culture Media / Q000737:chemistry*; D003839:Deoxyadenosines / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D012995:Solubility; D013696:Temperature
chemical_list: D003470:Culture Media; D003839:Deoxyadenosines; C058120:cordycepin
keywords: Cordycepin; Cordyceps militaris mutant; Crystallization; Proton beam irradiation; Purification