title: Coupling of Spinosad Fermentation and Separation Process via Two-Step Macroporous Resin Adsorption Method.
abstract: In this paper, a two-step resin adsorption technology was investigated for spinosad production and separation as follows: the first step resin addition into the fermentor at early cultivation period to decrease the timely product concentration in the broth; the second step of resin addition was used after fermentation to adsorb and extract the spinosad. Based on this, a two-step macroporous resin adsorption-membrane separation process for spinosad fermentation, separation, and purification was established. Spinosad concentration in 5-L fermentor increased by 14.45 % after adding 50 g/L macroporous at the beginning of fermentation. The established two-step macroporous resin adsorption-membrane separation process got the 95.43 % purity and 87 % yield for spinosad, which were both higher than that of the conventional crystallization of spinosad from aqueous phase that were 93.23 and 79.15 % separately. The two-step macroporous resin adsorption method has not only carried out the coupling of spinosad fermentation and separation but also increased spinosad productivity. In addition, the two-step macroporous resin adsorption-membrane separation process performs better in spinosad yield and purity.
authors.affiliation: Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Fanglong
authors.initials: F
authors.lastname: Zhao
authors.forename: Chuanbo
authors.initials: C
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Yin
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Shen
authors.forename: Wenyu
authors.initials: W
authors.lastname: Lu
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002853:Chromatography, Liquid; D003460:Crystallization; D004338:Drug Combinations; D005285:Fermentation; D018942:Macrolides / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008567:Membranes, Artificial; D011136:Polysorbates; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D014867:Water
chemical_list: D004338:Drug Combinations; D018942:Macrolides; D008567:Membranes, Artificial; D011136:Polysorbates; D012117:Resins, Synthetic; D014867:Water; C415329:spinosad