title: Up-Regulation of Corticocerebral NKD2 in Lipopolysaccharide-Induced Neuroinflammation.
abstract: Naked2 (NKD2), one member of Naked family, has been shown to negatively regulate Wnt/β-catenin signaling pathway. It has been recognized that NKD2 plays a vital role in cell homeostasis and prevention of tumorigenesis. However, NKD2 expression and its functional role in the brain in neuroinflammatory processes remain unclear. In our study, we investigated NKD2 distribution and role in lipopolysaccharide (LPS)-induced neuroinflammation rat model. The data indicated that NKD2 was up-regulated in LPS-injected brain, and the cellular localization of NKD2 was predominantly in cerebral cortical neurons. Furthermore, we treated primary neurons with conditioned media (CM) collected from LPS-stimulated mixed glial cultures (MGC). We detected that the up-regulation of NKD2 might be associated with the subsequent apoptosis in neurons. We also found knockdown NKD2 partially depressed the increase of cleaved caspase-3 and increased the reduction of β-catenin stimulated by MGC-CM. Taken together, these results suggested that NKD2 might be involved in neuronal apoptosis via the Wnt/β-catenin pathway during neuroinflammation in CNS. Our findings might provide a new therapeutic target for the prevention of neuroinflammation-involved neurological disorders.
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226002, Jiangsu, People's Republic of China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Pharmacology, Pharmacy College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Pharmacy College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Laboratory Animal Center, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226002, Jiangsu, People's Republic of China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226002, Jiangsu, People's Republic of China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226002, Jiangsu, People's Republic of China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226002, Jiangsu, People's Republic of China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xia
authors.affiliation: The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Pharmacology, Pharmacy College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Affiliated Cancer Hospital of Nantong University, Nantong, 226361, Jiangsu, People's Republic of China.
authors.forename: Yuejiao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. eudora2008@126.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002540:Cerebral Cortex / Q000473:pathology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D020868:Gene Silencing / Q000187:drug effects; D007249:Inflammation / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000473:pathology*; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; C000611390:NKD2 protein, rat
keywords: Lipopolysaccharide; NKD2; Neuroinflammation; Neuronal apoptosis; β-catenin