title: Insulin-Like Growth Factor Binding Protein-2 Promotes Adhesion of Endothelial Progenitor Cells to Endothelial Cells via Integrin α5β1.
abstract: The contribution of endothelial progenitor cells (EPCs) to new vessel formation has been studied in different physiological and pathological conditions for decades. As previously suggested, insulin-like growth factor binding protein-2 (IGFBP-2) may interact with integrins and promote cell migration. However, the role of IGFBP-2 in regulation of EPC functions remains largely unknown. In this present study, we found that overexpression of IGFBP-2 in human umbilical vein endothelial cells (HUVECs) promoted EPC-endothelial adhesion. Conversely, siRNA-mediated depletion of IGFBP-2 inhibited oxygen-glucose deprivation (OGD)-induced EPC-endothelial adhesion. Further, we demonstrated that the arginine-glycine-aspartic acid (RGD) motif in its C-domain is required for interaction with integrin α5β1. In addition, treatment with IGFBP-2 significantly enhanced incorporation of EPCs into tubule networks formed by HUVECs. Thus, our findings suggest that exogenous administration of IGFBP-2 may facilitate neovascularization and improve treatment of ischemic conditions.
authors.affiliation: Department of Neurology, Second Clinical Hospital, Harbin Medical University, 246 Xuefu Road, Harbin, 150086, People's Republic of China.
authors.forename: Nianping
authors.initials: N
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People's Republic of China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cerebropathy, Qiqihaer City Hospital of Traditional Chinese Medicine, Qiqihaer, 161000, China.
authors.forename: Zhengfei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Animal Experiment Center, Second Clinical Hospital, Harbin Medical University, Harbin, 150086, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Second Clinical Hospital, Harbin Medical University, Harbin, 150086, China. qfj2015@126.com.
authors.forename: Fujun
authors.initials: F
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People's Republic of China. hyd20132014@126.com.
authors.forename: Xijun
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People's Republic of China. f12905087@126.com.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Wang
mesh_terms: D020816:Amino Acid Motifs; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D015687:Cell Hypoxia; D003470:Culture Media; D042783:Endothelial Cells / Q000166:cytology*; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D005947:Glucose / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D039081:Integrin alpha5beta1 / Q000737:chemistry / Q000502:physiology*; D009842:Oligopeptides / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D025941:Protein Interaction Mapping; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D018971:Insulin-Like Growth Factor Binding Protein 2; D039081:Integrin alpha5beta1; D009842:Oligopeptides; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C047981:arginyl-glycyl-aspartic acid; D005947:Glucose; D010100:Oxygen
keywords: Endothelial progenitor cell; IGF binding protein 2; Integrin; Neovascularization; RGD motif