title: Metabolic fingerprinting of Arabidopsis thaliana accessions.
abstract: In the post-genomic era much effort has been put on the discovery of gene function using functional genomics. Despite the advances achieved by these technologies in the understanding of gene function at the genomic and proteomic level, there is still a big genotype-phenotype gap. Metabolic profiling has been used to analyze organisms that have already been characterized genetically. However, there is a small number of studies comparing the metabolic profile of different tissues of distinct accessions. Here, we report the detection of over 14,000 and 17,000 features in inflorescences and leaves, respectively, in two widely used Arabidopsis thaliana accessions. A predictive Random Forest Model was developed, which was able to reliably classify tissue type and accession of samples based on LC-MS profile. Thereby we demonstrate that the morphological differences among A. thaliana accessions are reflected also as distinct metabolic phenotypes within leaves and inflorescences.
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN) Irapuato, México ; Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República Montevideo, Uruguay.
authors.forename: Mariana
authors.initials: M
authors.lastname: Sotelo-Silveira
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN) Irapuato, México.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Chauvin
authors.affiliation: Department of Biotechnology and Biochemistry, CINVESTAV Unidad Irapuato Irapuato, Mexico.
authors.forename: Nayelli
authors.initials: N
authors.lastname: Marsch-Martínez
authors.affiliation: Department of Biotechnology and Biochemistry, CINVESTAV Unidad Irapuato Irapuato, Mexico.
authors.forename: Robert
authors.initials: R
authors.lastname: Winkler
authors.affiliation: Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN) Irapuato, México.
authors.forename: Stefan
authors.initials: S
authors.lastname: de Folter
keywords: Arabidopsis; accessions; development; metabolic phenotyping; metabolites