title: Amburana cearensis leaf extract maintains survival and promotes in vitro development of ovine secondary follicles.
abstract: The antioxidant properties of Amburana cearensis extract may be a useful substitute for standard cell culture medium. Thus, the aim of this study was to evaluate the effect of this extract, with or without supplementation, on in vitro survival and development of sheep isolated secondary follicles. After collection of the ovaries, secondary follicles were isolated and cultured for 18 days in α-MEM+ supplemented with bovine serum albumin, insulin, transferrin, selenium, glutamine, hypoxanthine and ascorbic acid (control medium) or into medium composed of different concentrations of A. cearensis extract without supplements (Amb 0.1; 0.2 or 0.4 mg/ml) or A. cearensis extract supplemented with the same substances described above for α-MEM+ supplementation. The A. cearensis supplemented medium was named Amb 0.1+; 0.2+ or 0.4+ mg/ml. There were more morphologically normal follicles in Amb 0.1 or Amb 0.4 mg/ml than in the control medium (α-MEM+) after 18 days of culture. Moreover, the percentage of antrum formation was significantly higher in Amb 0.1 or Amb 0.2 mg/ml than in α-MEM+ and Amb 0.1+ mg/ml, and similar to the other treatments. All A. cearensis extract media induced a progressive and significant increase in follicular diameter throughout the culture period. In conclusion, this study showed that 0.1 mg/ml of this extract, without supplementation, maintains follicular survival and promotes the development of ovine isolated secondary follicles in vitro. This extract can be an alternative culture medium for preantral follicle development.
authors.affiliation: Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: R S
authors.initials: RS
authors.lastname: Barberino
authors.affiliation: Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: V R P
authors.initials: VR
authors.lastname: Barros
authors.affiliation: Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: V G
authors.initials: VG
authors.lastname: Menezes
authors.affiliation: Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: L P
authors.initials: LP
authors.lastname: Santos
authors.affiliation: Faculty of Veterinary Medicine,Laboratory of Manipulation of Oocytes and Preantral Follicles,State University of Ceara,Fortaleza,CE,Brazil.
authors.forename: V R
authors.initials: VR
authors.lastname: Araújo
authors.affiliation: Laboratory of Bromatology and Animal Nutrition,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: M A A
authors.initials: MA
authors.lastname: Queiroz
authors.affiliation: Nucleus for Studies and Research on Medicinal Plants,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: J R G S
authors.initials: JR
authors.lastname: Almeida
authors.affiliation: Laboratory of Pharmacology,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Palheta
authors.affiliation: Universidade Federal do Vale do São Francisco (UNIVASF).Campus de Ciências Agrárias.Colegiado de Medicina Veterinária.Laboratório de Biologia Celular,Citologia e Histologia.Rodovia BR 407,Km 12,Lote 543 - Projeto de Irrigação Nilo Coelho - S/N,C1. CEP: 56300-990,Petrolina,PE,Brasil.
authors.forename: M H T
authors.initials: MH
authors.lastname: Matos
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D005973:Glutamine / Q000494:pharmacology; D019271:Hypoxanthine / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012643:Selenium / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012756:Sheep; D014168:Transferrin / Q000494:pharmacology
chemical_list: D007328:Insulin; D010936:Plant Extracts; D014168:Transferrin; D005973:Glutamine; D012710:Serum Albumin, Bovine; D019271:Hypoxanthine; D012643:Selenium; D001205:Ascorbic Acid
keywords: Culture; Medicinal plant; Oocyte; Sheep; Umburana