title: Inositols affect the mating circadian rhythm of Drosophila melanogaster.
abstract: Accumulating evidence indicates that the molecular circadian clock underlies the mating behavior of Drosophila melanogaster. However, information about which food components affect circadian mating behavior is scant. The ice plant, Mesembryanthemum crystallinum has recently become a popular functional food. Here, we showed that the close-proximity (CP) rhythm of D. melanogaster courtship behavior was damped under low-nutrient conditions, but significantly enhanced by feeding the flies with powdered ice plant. Among various components of ice plants, we found that myo-inositol increased the amplitude and slightly shortened the period of the CP rhythm. Real-time reporter assays showed that myo-inositol and D-pinitol shortened the period of the circadian reporter gene Per2-luc in NIH 3T3 cells. These data suggest that the ice plant is a useful functional food and that the ability of inositols to shorten rhythms is a general phenomenon in insects as well as mammals.
authors.affiliation: Ishida Group of Clock Genes, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology Tsukuba, Japan ; Graduate School of Life and Environmental Sciences, University of Tsukuba Tsukuba, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Sakata
authors.affiliation: Ishida Group of Clock Genes, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology Tsukuba, Japan.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Ishida Group of Clock Genes, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology Tsukuba, Japan ; SHIGRAY Inc. Sumida, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Ishida Group of Clock Genes, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology Tsukuba, Japan ; Graduate School of Life and Environmental Sciences, University of Tsukuba Tsukuba, Japan.
authors.forename: Kumpei
authors.initials: K
authors.lastname: Ito
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba Tsukuba, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Negishi
authors.affiliation: Tsuno Food Industrial Co. Ltd. Wakayama, Japan.
authors.forename: Takuo
authors.initials: T
authors.lastname: Tsuno
authors.affiliation: Tsuno Food Industrial Co. Ltd. Wakayama, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Tsuno
authors.affiliation: Tsuno Food Industrial Co. Ltd. Wakayama, Japan.
authors.forename: Youta
authors.initials: Y
authors.lastname: Yamazaki
authors.affiliation: Ishida Group of Clock Genes, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology Tsukuba, Japan ; Graduate School of Life and Environmental Sciences, University of Tsukuba Tsukuba, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Ishida
keywords: Drosophila melanogaster; circadian rhythm; ice plant; mating succession; myo-inositol