title: Preparation of silica nanoparticles loaded with nootropics and their in vivo permeation through blood-brain barrier.
abstract: The blood-brain barrier prevents the passage of many drugs that target the central nervous system. This paper presents the preparation and characterization of silica-based nanocarriers loaded with piracetam, pentoxifylline, and pyridoxine (drugs from the class of nootropics), which are designed to enhance the permeation of the drugs from the circulatory system through the blood-brain barrier. Their permeation was compared with non-nanoparticle drug substances (bulk materials) by means of an in vivo model of rat brain perfusion. The size and morphology of the nanoparticles were characterized by transmission electron microscopy. The content of the drug substances in silica-based nanocarriers was analysed by elemental analysis and UV spectrometry. Microscopic analysis of visualized silica nanocarriers in the perfused brain tissue was performed. The concentration of the drug substances in the tissue was determined by means of UHPLC-DAD/HRMS LTQ Orbitrap XL. It was found that the drug substances in silica-based nanocarriers permeated through the blood brain barrier to the brain tissue, whereas bulk materials were not detected in the brain.
authors.affiliation: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic.
authors.forename: Josef
authors.identifier: 0000-0003-2003-9052
authors.initials: J
authors.lastname: Jampilek
authors.affiliation: Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Kamil
authors.initials: K
authors.lastname: Zaruba
authors.affiliation: Global Change Research Centre AS CR, Belidla 986/4a, 603 00 Brno, Czech Republic.
authors.forename: Michal
authors.identifier: 0000-0002-2506-5826
authors.initials: M
authors.lastname: Oravec
authors.affiliation: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Kunes
authors.affiliation: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic.
authors.forename: Petr
authors.identifier: 0000-0003-2389-2868
authors.initials: P
authors.lastname: Babula
authors.affiliation: Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Ulbrich
authors.affiliation: Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Brezaniova
authors.affiliation: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic.
authors.forename: Radka
authors.identifier: 0000-0002-5945-2082
authors.initials: R
authors.lastname: Opatrilova
authors.affiliation: Global Change Research Centre AS CR, Belidla 986/4a, 603 00 Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Triska
authors.affiliation: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Suchy
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D018697:Nootropic Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D004337:Drug Carriers; D018697:Nootropic Agents; D012822:Silicon Dioxide