title: Hepatotoxicity of herbicide Sencor in goldfish may result from induction of mild oxidative stress.
abstract: The effects of 96 h exposure to 7.14, 35.7, or 71.4 mg L(-1) of Sencor were studied on liver and plasma parameters in goldfish, Carassius auratus L. Goldfish exposure to 71.4 mg L(-1) of Sencor for 96 h resulted in a decrease in glucose concentrations in plasma and liver by 55%, but did not affect liver glycogen levels. An increase in the activity of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase (by 24-27%, 32-72%, and 87-102%, respectively) occurred in plasma of Sencor exposed goldfish, whereas in liver activities of these enzymes decreased (by 15-17%, 19%, and 20%, respectively). Lactate concentration in plasma increased by 22-36% in all treated fish groups, whereas in liver it increased by 64% only after exposure to 35.7 mg L(-1) of Sencor. Herbicide exposure enhanced lipid peroxide levels by 49-75% and decreased activities of catalase by 46%, glutathione reductase by 25-48% and glutathione peroxidase by 21-26% suggesting development of oxidative stress in liver. The treatment induced various histological changes in goldfish liver, such as dilated sinusoids, hypertrophy and dystrophy of hepatic cells and detachment of endothelial cytoplasm with diffuse hemorrhage. The data collectively let us propose that mild oxidative stress might be responsible for the hepatotoxicity of Sencor.
authors.affiliation: Department of Biochemistry and Biotechnology, Precarpathian National University named after Vasyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine.
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Maksymiv
authors.affiliation: Department of Biochemistry and Biotechnology, Precarpathian National University named after Vasyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine.
authors.forename: Viktor V
authors.initials: VV
authors.lastname: Husak
authors.affiliation: Department of Biochemistry and Biotechnology, Precarpathian National University named after Vasyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine.
authors.forename: Nadia M
authors.initials: NM
authors.lastname: Mosiichuk
authors.affiliation: Department of Biochemistry and Biotechnology, Precarpathian National University named after Vasyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine.
authors.forename: Tetiana M
authors.initials: TM
authors.lastname: Matviishyn
authors.affiliation: Department of Human and Animal Anatomy and Physiology, Precarpathian National University named after Vasyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine.
authors.forename: Iryna Y
authors.initials: IY
authors.lastname: Sluchyk
authors.affiliation: Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Storey
authors.affiliation: Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Storey
authors.affiliation: Department of Biochemistry and Biotechnology, Precarpathian National University named after Vasyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine. Electronic address: lushchak@pu.if.ua.
authors.forename: Volodymyr I
authors.initials: VI
authors.lastname: Lushchak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001769:Blood / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity; D007773:Lactates / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects*; D014227:Triazines / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D006540:Herbicides; D007773:Lactates; D014227:Triazines; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; C009235:metribuzin
keywords: Blood parameters; Carassius auratus; Histology; Liver; Metribuzin; Oxidative stress