title: Cartilage-Specific Near-Infrared Fluorophores for Biomedical Imaging.
abstract: A novel class of near-infrared fluorescent contrast agents was developed. These agents target cartilage with high specificity and this property is inherent to the chemical structure of the fluorophore. After a single low-dose intravenous injection and a clearance time of approximately 4 h, these agents bind to all three major types of cartilage (hyaline, elastic, and fibrocartilage) and perform equally well across species. Analysis of the chemical structure similarities revealed a potential pharmacophore for cartilage targeting. Our results lay the foundation for future improvements in tissue engineering, joint surgery, and cartilage-specific drug development.
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, SL436A, Boston, MA 02215 (USA).|Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 501-746 (South Korea).
authors.forename: Hoon
authors.initials: H
authors.lastname: Hyun
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303 (USA).
authors.forename: Eric A
authors.initials: EA
authors.lastname: Owens
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, SL436A, Boston, MA 02215 (USA).
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Wada
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303 (USA).
authors.forename: Andrew
authors.initials: A
authors.lastname: Levitz
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, SL436A, Boston, MA 02215 (USA).
authors.forename: GwangLi
authors.initials: G
authors.lastname: Park
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, SL436A, Boston, MA 02215 (USA).|Department of Surgery, Chonnam National University Medical School, Gwangju 501-746 (South Korea).
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Park
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, SL436A, Boston, MA 02215 (USA).|Curadel, LLC, 377 Plantation Street, Worcester, MA 01605 (USA).
authors.forename: John V
authors.initials: JV
authors.lastname: Frangioni
authors.affiliation: Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303 (USA). mhenary1@gsu.edu.
authors.forename: Maged
authors.initials: M
authors.lastname: Henary
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, SL436A, Boston, MA 02215 (USA). hchoi@bidmc.harvard.edu.|Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735 (South Korea). hchoi@bidmc.harvard.edu.
authors.forename: Hak Soo
authors.initials: HS
authors.lastname: Choi
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D061848:Optical Imaging / Q000379:methods; D019265:Spectroscopy, Near-Infrared
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes
keywords: NIR fluorescence; cartilage; fluorophores; imaging agents; targeted dyes