title: Recombinant Phage Coated 1D Al2O3 Nanostructures for Controlling the Adhesion and Proliferation of Endothelial Cells.
abstract: A novel synthesis of a nanostructured cell adhesive surface is investigated for future stent developments. One-dimensional (1D) Al2O3 nanostructures were prepared by chemical vapor deposition of a single source precursor. Afterwards, recombinant filamentous bacteriophages which display a short binding motif with a cell adhesive peptide (RGD) on p3 and p8 proteins were immobilized on these 1D Al2O3 nanostructures by a simple dip-coating process to study the cellular response of human endothelial EA hy.926. While the cell density decreased on as-deposited 1D Al2O3 nanostructures, we observed enhanced cell proliferation and cell-cell interaction on recombinant phage overcoated 1D Al2O3 nanostructures. The recombinant phage overcoating also supports an isotropic cell spreading rather than elongated cell morphology as we observed on as-deposited Al2O3 1D nanostructures.
authors.affiliation: INM-Leibniz-Institute for New Materials, Campus D2 2, 66123 Saarbrücken, Germany.
authors.forename: Juseok
authors.initials: J
authors.lastname: Lee
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Hojeong
authors.initials: H
authors.lastname: Jeon
authors.affiliation: Clinic for Pediatric Cardiology, Saarland University Hospital, 66421 Homburg, Germany.
authors.forename: Ayman
authors.initials: A
authors.lastname: Haidar
authors.affiliation: Clinic for Pediatric Cardiology, Saarland University Hospital, 66421 Homburg, Germany.
authors.forename: Hashim
authors.initials: H
authors.lastname: Abdul-Khaliq
authors.affiliation: Inorganic Chemistry Department, Saarland University Campus C4 1, 66123 Saarbrücken, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Veith
authors.affiliation: INM-Leibniz-Institute for New Materials, Campus D2 2, 66123 Saarbrücken, Germany.
authors.forename: Cenk
authors.identifier: 0000-0002-8482-2030
authors.initials: C
authors.lastname: Aktas
authors.affiliation: Korea Institute of Science and Technology Europe (KIST-Europe) Forschungsgesellschaft mbH, Campus E 7 1, 66123 Saarbrücken, Germany.
authors.forename: Youngjun
authors.initials: Y
authors.lastname: Kim
mesh_terms: D001435:Bacteriophages / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry*; D013499:Surface Properties