title: [Anti-attachment effect of ethyl acetate extract of Huanglian Jiedu decoction on Candida glabrata].
abstract: OBJECTIVE
  To investigate anti-attachment effect of ethyl acetate extract of Huanglian Jiedu decoction (EAHD) on Candida glabrata.
  
  
  METHODS
  Serial 2-fold dilution assay was used to determine the minimum inhibitory concentrations MICs of EAHD to C. glabrata. XTT assay was used to evaluate the effect of EAHD against adhesion of C. glabrata. Inverted microscope, scanning electron microscope (SEM) and fluorescein diacetate (FDA) staining were applied to observe the morphological changes of C. glabrata in adhesion. PCR was adopted to inspect the expression of attachment-related genes such as EPA1, EPA6 and EPA7.
  
  
  RESULTS
  The MIC of EAHD and fluconazole to C. glabrata were 320 mg · L(-1) and 1 mg · L(-1) respectively. The total cells including budding cells decreased in a dose-dependent manner following EAHD treatment. The expressions of EPA1, EPA6 and EPA7 were downregulated dramatically after EAHD treatment.
  
  
  CONCLUSIONS
  EAHD could effectively inhibit adherence of C. glabrata.
authors.forename: Tian-ming
authors.initials: TM
authors.lastname: Wang
authors.forename: Meng-xiang
authors.initials: MX
authors.lastname: Zhang
authors.forename: Gao-xiang
authors.initials: GX
authors.lastname: Shi
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Dan
authors.initials: D
authors.lastname: Xia
authors.forename: Chang-zhong
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000085:Acetates; D041221:Candida glabrata / Q000187:drug effects* / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics; D037102:Lectins / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000085:Acetates; D004365:Drugs, Chinese Herbal; D005656:Fungal Proteins; D037102:Lectins; D010936:Plant Extracts; C120505:epithelial adhesin 1, Candida glabrata; C494417:huanglian; C007650:ethyl acetate