title: Imaging of tumor clones with differential liver colonization.
abstract: We present a model of hepatic colorectal metastases which represents monoclonal cell lines double-labeled by luciferase and tdTomato. These cells form liver metastasis in varying numbers and patterns similar to those observed in patients. Using in vivo and ex vivo luminescent and fluorescent imaging we determine the growth kinetics and clonogenic frequency of tumor cells colonizing liver. Molecular profiling detected stable expressional differences between clones consistent with their phenotypes. The data indicate that clinically relevant phenotypes of liver metastases can be modeled in vivo.
authors.affiliation: 1] Department of Surgery [2] Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Go
authors.initials: G
authors.lastname: Oshima
authors.affiliation: Department of Surgery.
authors.forename: Sean C
authors.initials: SC
authors.lastname: Wightman
authors.affiliation: Department of Surgery.
authors.forename: Abhineet
authors.initials: A
authors.lastname: Uppal
authors.affiliation: Department of Surgery.
authors.forename: Melinda E
authors.initials: ME
authors.lastname: Stack
authors.affiliation: Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Pitroda
authors.affiliation: Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Oskvarek
authors.affiliation: Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Surgery.
authors.forename: Mitchell C
authors.initials: MC
authors.lastname: Posner
authors.affiliation: Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Samuel
authors.initials: S
authors.lastname: Hellman
authors.affiliation: Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Ralph R
authors.initials: RR
authors.lastname: Weichselbaum
authors.affiliation: Department of Radiation and Cellular Oncology, Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637.
authors.forename: Nikolai N
authors.initials: NN
authors.lastname: Khodarev
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D064593:Heterografts; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D008163:Luminescent Measurements / Q000379:methods; D047368:Tumor Burden