title: CKA2 functions in H2O2-induced apoptosis and high-temperature stress tolerance by regulating NO accumulation in yeast.
abstract: Nitric oxide (NO) plays key roles in yeast responses to various environmental factors, such as H2O2 and high temperature. However, the gene encoding NO synthase (NOS) in yeast has not yet been identified, and the mechanism underlying the regulation of NOS-like activity is poorly understood. Here, we report on the involvement of CKA2 in H2O2-induced yeast apoptosis and yeast high-temperature stress tolerance. Our results showed that although Δcka2 mutant had reduced NO accumulation with decreased apoptosis after H2O2 exposure, treatment with a NO donor, sodium nitroprusside, resulted in similar survival rate of Δcka2 mutant compared to that of wild-type yeast when subjected to H2O2 stress. This finding occurred because H2O2-enhanced NOS-like activity in wild-type yeast was significantly repressed in Δcka2. Our additional experiments indicated that both high-temperature-enhanced NO accumulation and NOS-like activity were also suppressed in Δcka2, leading to the hypersensitivity of the mutant to high temperature in terms of changes in survival rate. Thus, our results showed that CKA2 functioned in H2O2-induced apoptosis and high-temperature stress tolerance by regulating NOS-like-dependent NO accumulation in yeast.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Liu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Yuan
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Li
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, China yingtlu@whu.edu.cn.
authors.forename: Ying-Tang
authors.initials: YT
authors.lastname: Lu
mesh_terms: D017209:Apoptosis; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D006358:Hot Temperature / Q000009:adverse effects*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D050296:Microbial Viability / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; C000593617:CKa2 protein, S cerevisiae; D047390:Casein Kinase II
keywords: CKA2; H2O2; NOS-like; high-temperature stress; nitric oxide; yeast