title: Rewriting the blueprint of life by synthetic genomics and genome engineering.
abstract: Advances in DNA synthesis and assembly methods over the past decade have made it possible to construct genome-size fragments from oligonucleotides. Early work focused on synthesis of small viral genomes, followed by hierarchical synthesis of wild-type bacterial genomes and subsequently on transplantation of synthesized bacterial genomes into closely related recipient strains. More recently, a synthetic designer version of yeast Saccharomyces cerevisiae chromosome III has been generated, with numerous changes from the wild-type sequence without having an impact on cell fitness and phenotype, suggesting plasticity of the yeast genome. A project to generate the first synthetic yeast genome--the Sc2.0 Project--is currently underway.
authors.affiliation: Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD, 21205, USA.
authors.forename: Narayana
authors.initials: N
authors.lastname: Annaluru
authors.affiliation: Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD, 21205, USA.
authors.forename: Sivaprakash
authors.initials: S
authors.lastname: Ramalingam
authors.affiliation: Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD, 21205, USA. chandra@jhmi.edu.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Chandrasegaran
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D016680:Genome, Bacterial; D016681:Genome, Fungal; D016679:Genome, Viral; D023281:Genomics / Q000379:methods; D005838:Genotype; D045704:Mycoplasma genitalium / Q000235:genetics; D010641:Phenotype; D017955:Poliovirus / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D004279:DNA, Viral; D011994:Recombinant Proteins; D003851:Deoxyribonucleases