title: [Protective effects of notoginsenoside R1 against amyloid-β (1-42) induced mitochondrial apopototic death in SH-SY5Y cells].
abstract: OBJECTIVE
  To investigate the effects and underlying mechanism of notoginsenoside R1 on amyloid-β (1-42) (Aβ(1-42)) induced mitochondrial apoptotic death in SH-SY5Y cells.
  
  
  METHODS
  Cell viability was assayed by MTT, apoptotic rates were analyzed with PI/Annexin V flow cytometry, Bax and Bcl-2 expression were detected with Western blotting, enzymatic activity of caspase-3, caspase-8 and caspase-9 were measured by ELISA assay.
  
  
  RESULTS
  The 6.25-100 nmol x L(-1) of notoginsenoside R1 attenuate Aβ(1-42) induced apoptotic death of SH-SY5Y in dose dependent manner. The ratio of Bcl-2/Bax was elevated in SH-SY5Y with notoginsenoside R1 treatment. Caspase-3 and caspase-9 were activated with notoginsenoside R1 treatment while caspase-8 was not affected.
  
  
  CONCLUSIONS
  Notoginsenoside R1 could protect SH-SY5Y cells from Aβ(1-42) induced apoptosis via mitochondria related apoptotic pathway.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Wen-feng
authors.initials: WF
authors.lastname: Xin
authors.forename: Wen-sheng
authors.initials: WS
authors.lastname: Zhang
authors.forename: Yong-yan
authors.initials: YY
authors.lastname: Wang
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D008928:Mitochondria / Q000187:drug effects*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors*
chemical_list: D016229:Amyloid beta-Peptides; D036145:Ginsenosides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D020169:Caspases; C072936:notoginsenoside R1