title: Application of next-generation sequencing for 24-chromosome aneuploidy screening of human preimplantation embryos.
abstract: BACKGROUND
  Aneuploidy is a leading cause of repeat implantation failure and recurrent miscarriages. Preimplantation genetic screening (PGS) enables the assessment of the numeral and structural chromosomal errors of embryos before transfer in patients undergoing in vitro fertilization. Array comparative genomic hybridization (aCGH) has been demonstrated to be an accurate PGS method and in present thought to be the gold standard, but new technologies, such as next-generation sequencing (NGS), continue to emerge. Validation of the new comprehensive NGS-based 24-chromosome aneuploidy screening technology is still needed to determine the preclinical accuracy before it might be considered as an alternative method for human PGS.
  
  
  RESULTS
  In the present study, 43 human trophectoderm (TE) biopsy samples and 5 cytogenetically characterized cell lines (Coriell Cell Repositories) were tested. The same whole genome amplified product of each sample was blindly assessed with Veriseq NGS and Agilent aCGH to identify the aneuploidy status. The result showed that the NGS identified all abnormalities identified in aCGH including the numeral chromosomal abnormalities (again or loss) in the embryo samples and the structural (partial deletion and duplication) in the Coriell cell lines. Both technologies can identify a segmental imbalance as small as 1.8 Mb in size. Among the 41 TE samples with abnormal karyotypes in this study, eight (19.5 %) samples presented as multiple chromosome abnormalities. The abnormalities occurred to almost all chromosomes, except chromosome 6, 7, 17 and Y chromosome.
  
  
  CONCLUSIONS
  Given its reliability and high level of consistency with an established aCGH methodology, NGS has demonstrated a robust high-throughput methodology ready for extensive clinical application in reproductive medicine, with potential advantages of reduced costs and enhanced precision. Then, a randomized controlled clinical trial confirming its clinical effectiveness is advisable to obtain a larger sequencing dataset and more evidence for the extensive use of NGS-based PGS.
authors.affiliation: Reproductive Medicine Center, Key Laboratory for Reproductive Medicine of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Pacgenomics Inc, Agoura Hills, CA USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.affiliation: Pacgenomics Inc, Agoura Hills, CA USA.
authors.forename: Lian
authors.initials: L
authors.lastname: Liu
authors.affiliation: Reproductive Medicine Center, Key Laboratory for Reproductive Medicine of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Houston Fertility Laboratory, Vivere Health, Houston, TX USA.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wang
keywords: Aneuploidy screening; Array comparative genomic hybridization; Blastocyst; Next-generation sequencing; Preimplantation genetic screening