title: Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. A comparative study.
abstract: This paper addresses a comparative study of immunomagnetic separation of Salmonella using micro and nano-sized magnetic carriers. In this approach, nano (300 nm) and micro (2.8 μm) sized magnetic particles were modified with anti-Salmonella antibody to pre-concentrate the bacteria from the samples throughout an immunological reaction. The performance of the immunomagnetic separation on the different magnetic carriers was evaluated using classical culturing, confocal and scanning electron microscopy to study the binding pattern, as well as a magneto-actuated immunosensor with electrochemical read-out for the rapid detection of the bacteria in spiked milk samples. In this approach, a second polyclonal antibody labeled with peroxidase as electrochemical reporter was used. The magneto-actuated electrochemical immunosensor was able to clearly distinguish between food pathogenic bacteria such as Salmonella enterica and Escherichia coli, showing a limit of detection (LOD) as low as 538 CFU mL(-1) and 291 CFU mL(-1) for magnetic micro and nanocarriers, respectively, in whole milk, although magnetic nanoparticles showed a noticeable higher matrix effect and higher agglomeration effect. These LODs were achieved in a total assay time of 1h without any previous culturing pre-enrichment step. If the samples were pre-enriched for 8 h, the magneto immunosensor based on the magnetic nanoparticles was able to detect as low as 1 CFU in 25 mL of milk (0.04 CFU mL(-1)).
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain.
authors.forename: Delfina
authors.initials: D
authors.lastname: Brandão
authors.affiliation: Applied Enzyme Technology Ltd., Gwent Group Ltd., Monmouth House, Mamhilad Park, Pontypool, NP4 OHZ, UK.
authors.forename: Susana
authors.initials: S
authors.lastname: Liébana
authors.affiliation: Grup de Microbiologia Molecular, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Campoy
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Alegret
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Bellaterra), Spain. Electronic address: Isabel.Pividori@uab.cat.
authors.forename: María
authors.initials: M
authors.lastname: Isabel Pividori
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D004563:Electrochemistry; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification; D018189:Immunomagnetic Separation / Q000379:methods*; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D008863:Microspheres; D008892:Milk / Q000382:microbiology; D053758:Nanoparticles / Q000737:chemistry*; D019779:Salmonella enterica / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized
keywords: Electrochemical immunosensor; Immunomagnetic separation; Magnetic nanoparticles; Salmonella enterica