title: A Proteomic Characterization of Bordetella pertussis Clinical Isolates Associated with a California State Pertussis Outbreak.
abstract: Bordetella pertussis (Bp) is the etiologic agent of pertussis (whooping cough), a highly communicable infection. Although pertussis is vaccine preventable, in recent years there has been increased incidence, despite high vaccine coverage. Possible reasons for the rise in cases include the following: Bp strain adaptation, waning vaccine immunity, increased surveillance, and improved clinical diagnostics. A pertussis outbreak impacted California (USA) in 2010; children and preadolescents were the most affected but the burden of disease fell mainly on infants. To identify protein biomarkers associated with this pertussis outbreak, we report a whole cellular protein characterization of six Bp isolates plus the pertussis acellular vaccine strain Bp Tohama I (T), utilizing gel-free proteomics-based mass spectrometry (MS). MS/MS tryptic peptide detection and protein database searching combined with western blot analysis revealed three Bp isolates in this study had markedly reduced detection of pertactin (Prn), a subunit of pertussis acellular vaccines. Additionally, antibody affinity capture technologies were implemented using anti-Bp T rabbit polyclonal antisera and whole cellular proteins to identify putative immunogens. Proteome profiling could shed light on pathogenesis and potentially lay the foundation for reduced infection transmission strategies and improved clinical diagnostics.
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: Yulanda M
authors.initials: YM
authors.lastname: Williamson
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: Hercules
authors.identifier: 0000-0001-7672-0942
authors.initials: H
authors.lastname: Moura
authors.affiliation: Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Whitmon
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Woolfitt
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Schieltz
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: Jon C
authors.initials: JC
authors.lastname: Rees
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA ; Oak Ridge Institute for Science and Education, Oak Ridge, TN 37831-0117, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Guo
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA ; Oak Ridge Institute for Science and Education, Oak Ridge, TN 37831-0117, USA.
authors.forename: Heather
authors.identifier: 0000-0001-9264-023X
authors.initials: H
authors.lastname: Kirkham
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA ; Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bouck
authors.affiliation: Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Edwin W
authors.initials: EW
authors.lastname: Ades
authors.affiliation: Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Tondella
authors.affiliation: Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: George M
authors.initials: GM
authors.lastname: Carlone
authors.affiliation: Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Jacquelyn S
authors.initials: JS
authors.lastname: Sampson
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Barr