title: Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release.
abstract: A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.
authors.affiliation: Key Laboratory of Nano-Bio Interfacce, Division of Nanobiomedicine, Suzhou Insitute of Nano-Tech and Nano-Bionics, Chinese Academy of Science, Suzhou, 215123, China. rjpei2011@sinano.ac.cn.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Shen
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.forename: Kewei
authors.initials: K
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001943:Breast Neoplasms / Q000201:enzymology; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005453:Fluorescence; D054856:G-Quadruplexes; D006801:Humans; D008027:Light; D010766:Phosphorylation; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology; D013050:Spectrometry, Fluorescence
chemical_list: D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase