title: Cytotoxic triterpene saponins from Cercodemas anceps.
abstract: Seven holostane-type triterpene saponins (1-7), including five new compounds namely cercodemasoides A-E (2-6), were isolated from the sea cucumber Cercodemas anceps. Their structures were elucidated on the basis of spectroscopic evidence including HR ESI MS, ESI MS/MS, 1D and 2D NMR. The cytotoxic effects of isolated compounds were evaluated by SRB method on five human cancer cell lines including Hep-G2 (hepatoma cancer), KB (epidermoid carcinoma), LNCaP (prostate cancer), MCF7 (breast cancer), and SK-Mel2 (melanoma). Compounds 1-7 showed potent cytotoxicity on five tested cancer cell lines with IC50 values ranging from 0.03 ± 0.01 to 7.36 ± 0.46 μM. With respect to the potent cytotoxicity of the isolated saponins, further studies are required to confirm efficacy in vivo and the mechanism of cytotoxic effects.
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Vien
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Tran Thi Hong
authors.initials: TT
authors.lastname: Hanh
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.affiliation: Institute of Biotechnology (IBT), VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Thao
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Thanh
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.affiliation: Institute of Marine Environment and Resources (IMER), VAST, 246 Da Nang, Haiphong, Viet Nam.
authors.forename: Do Cong
authors.initials: do C
authors.lastname: Thung
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.affiliation: Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam. Electronic address: cvminh@vast.vn.
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D012616:Sea Cucumbers / Q000737:chemistry* / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry
chemical_list: D012503:Saponins; D014315:Triterpenes
keywords: Cercodemas anceps; Cercodemasoide; Cucumariidae; Cytotoxic activity; Sea cucumber