title: Method for generation of peptide-specific IgY antibodies directed to Staphylococcus aureus extracellular fibrinogen binding protein epitope.
abstract: The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococcus aureus fibrinogen binding protein (Efb) and its peptidyl epitope (spanning residues 127-140). The Efb is an extracellular, adhesion protein which binds both human fibrinogen and complement C3 protein thus contributing to the high infectious potential of this pathogen. The selected epitope of Efb protein is responsible for the interaction with C3. The immunochemical characterization of both anti-Efb and epitope-specific IgY antibodies revealed their similar avidity, titer, and reactivity profile, although some differences in the hen's immune response to administered antigens is discussed.
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Walczak
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Grzywa
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Łupicka-Słowik
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Skoreński
authors.affiliation: Department of Epizootiology and Clinic of Bird and Exotic Animals, Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Pl. Grunwaldzki 45, 50-366, Wroclaw, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Bobrek
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Nowak
authors.affiliation: European Molecular Biology Laboratory (EMBL), Hamburg Outstation, Notkestrasse 85, 22607, Hamburg, Germany.
authors.forename: Stephane
authors.initials: S
authors.lastname: Boivin
authors.affiliation: Center for Infectious Disease, Division of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas Health Science Center, Houston, TX, USA.
authors.forename: Eric L
authors.initials: EL
authors.lastname: Brown
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Józef
authors.initials: J
authors.lastname: Oleksyszyn
authors.affiliation: Division of Medicinal Chemistry and Microbiology, Wroclaw University of Technology, Faculty of Chemistry, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Sieńczyk
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology; D002645:Chickens; D000939:Epitopes / Q000276:immunology; D005260:Female; D005340:Fibrinogen / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D010455:Peptides / Q000276:immunology; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D000939:Epitopes; C016512:IgY; D007136:Immunoglobulins; D010455:Peptides; D005340:Fibrinogen
keywords: IgY immunoglobulins; Staphylococcus aureus; complement C3; fibrinogen binding protein; peptidyl epitope