title: Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System.
abstract: The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.
authors.affiliation: Department of Biomedical Engineering, University of California Irvine, CA, USA lxkong@uci.edu.
authors.forename: Ling X
authors.initials: LX
authors.lastname: Kong
authors.affiliation: Department of Physics and Astronomy, University of California Irvine, Irvine, CA, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Perebikovsky
authors.affiliation: Department of Biomedical Engineering, University of California Irvine, CA, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Moebius
authors.affiliation: Department of Mechanical Engineering, University of California Irvine, Irvine, CA, USA.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Kulinsky
authors.affiliation: Department of Biomedical Engineering, University of California Irvine, CA, USA Department of Mechanical Engineering, University of California Irvine, Irvine, CA, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Madou
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D044085:Microfluidics / Q000379:methods* / Q000639:trends; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000639:trends; D013048:Specimen Handling / Q000379:methods / Q000639:trends
keywords: LoD; analytical; assay; centrifugal; microfluidics; point-of-care; sample-to-answer