title: Three-dimensional Nanowire Structures for Ultra-Fast Separation of DNA, Protein and RNA Molecules.
abstract: Separation and analysis of biomolecules represent crucial processes for biological and biomedical engineering development; however, separation resolution and speed for biomolecules analysis still require improvements. To achieve separation and analysis of biomolecules in a short time, the use of highly-ordered nanostructures fabricated by top-down or bottom-up approaches have been proposed. Here, we reported on the use of three-dimensional (3D) nanowire structures embedded in microchannels fabricated by a bottom-up approach for ultrafast separation of small biomolecules, such as DNA, protein, and RNA molecules. The 3D nanowire structures could analyze a mixture of DNA molecules (50-1000 bp) within 50 s, a mixture of protein molecules (20-340 kDa) within 5 s, and a mixture of RNA molecules (100-1000 bases) within 25 s. And, we could observe the electrophoretic mobility difference of biomolecules as a function of molecular size in the 3D nanowire structures. Since the present methodology allows users to control the pore size of sieving materials by varying the number of cycles for nanowire growth, the 3D nanowire structures have a good potential for use as alternatives for other sieving materials.
authors.affiliation: 1] Institute of Innovation for Future Society, Nagoya University, JAPAN [2] FIRST Research Center for Innovative Nanobiodevices, Nagoya University, JAPAN.
authors.forename: Sakon
authors.initials: S
authors.lastname: Rahong
authors.affiliation: 1] FIRST Research Center for Innovative Nanobiodevices, Nagoya University, JAPAN [2] Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, JAPAN.
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagida
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nagashima
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kanai
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Gang
authors.initials: G
authors.lastname: Meng
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Zhuge
authors.affiliation: 1] FIRST Research Center for Innovative Nanobiodevices, Nagoya University, JAPAN [2] Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, JAPAN.
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, JAPAN.
authors.forename: Tomoji
authors.initials: T
authors.lastname: Kawai
authors.affiliation: 1] Institute of Innovation for Future Society, Nagoya University, JAPAN [2] FIRST Research Center for Innovative Nanobiodevices, Nagoya University, JAPAN [3] Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, JAPAN [4] Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), JAPAN.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D001583:Benzoxazoles; D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D053770:Nanowires / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D011806:Quinolinium Compounds; D012313:RNA / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D001583:Benzoxazoles; D005456:Fluorescent Dyes; D011506:Proteins; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D012313:RNA; D004247:DNA