title: Hydrogen Sulfide Alleviates Cadmium-Induced Cell Death through Restraining ROS Accumulation in Roots of Brassica rapa L. ssp. pekinensis.
abstract: Hydrogen sulfide (H2S) is a cell signal molecule produced endogenously and involved in regulation of tolerance to biotic and abiotic stress in plants. In this work, we used molecular biology, physiology, and histochemical methods to investigate the effects of H2S on cadmium- (Cd-) induced cell death in Chinese cabbage roots. Cd stress stimulated a rapid increase of endogenous H2S in roots. Additionally, root length was closely related to the cell death rate. Pretreatment with sodium hydrosulfide (NaHS), a H2S donor, alleviated the growth inhibition caused by Cd in roots-this effect was more pronounced at 5 μM NaHS. Cd-induced cell death in roots was significantly reduced by 5 μM NaHS treatment. Under Cd stress, activities of the antioxidant enzymes were significantly enhanced in roots. NaHS + Cd treatment made their activities increase further compared with Cd exposure alone. Enhanced antioxidant enzyme activity led to a decline in reactive oxygen species accumulation and lipid peroxidation. In contrast, these effects were reversed by hydroxylamine, a H2S inhibitor. These results suggested that H2S alleviated the cell death caused by Cd via upregulation of antioxidant enzyme activities to remove excessive reactive oxygen species and reduce cell oxidative damage.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Liping
authors.identifier: 0000-0002-0261-719X
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Yanxi
authors.initials: Y
authors.lastname: Pei
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Hongjiao
authors.identifier: 0000-0002-5559-8041
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zhuping
authors.identifier: 0000-0002-5533-6201
authors.initials: Z
authors.lastname: Jin
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zhiqiang
authors.identifier: 0000-0002-1421-0290
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zengjie
authors.identifier: 0000-0001-5498-0364
authors.initials: Z
authors.lastname: Qiao
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Huihui
authors.identifier: 0000-0002-1774-2436
authors.initials: H
authors.lastname: Fang
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Yanjie
authors.identifier: 0000-0001-8142-3029
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001937:Brassica / Q000187:drug effects / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013440:Sulfides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013440:Sulfides; D002104:Cadmium; D008315:Malondialdehyde; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; C025451:sodium bisulfide