title: Magnetic nanoparticles enhance the anticancer activity of cathelicidin LL-37 peptide against colon cancer cells.
abstract: The pleiotropic activity of human cathelicidin LL-37 peptide includes an ability to suppress development of colon cancer cells. We hypothesized that the anticancer activity of LL-37 would improve when attached to the surface of magnetic nanoparticles (MNPs). Using colon cancer culture (DLD-1 cells and HT-29 cells), we evaluated the effects of MNPs, LL-37 peptide, its synthetic analog ceragenin CSA-13, and two novel nanosystems, ie, MNP@LL-37 and MNP@CSA-13, on cancer cell viability and apoptosis. Treatment of cancer cells with the LL-37 peptide linked to MNPs (MNP@LL-37) caused a greater decrease in cell viability and a higher rate of apoptosis compared with treatment using free LL-37 peptide. Additionally, we observed a strong ability of ceragenin CSA-13 and MNP@CSA-13 to induce apoptosis of DLD-1 cells. We found that both nanosystems were successfully internalized by HT-29 cells, and cathelicidin LL-37 and ceragenin CSA-13 might play a key role as novel homing molecules. These results indicate that the previously described anticancer activity of LL-37 peptide against colon cancer cells might be significantly improved using a theranostic approach.
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Niemirowicz
authors.affiliation: Department of Medicinal Chemistry, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Prokop
authors.affiliation: Institute of Chemistry, University of Bialystok, Bialystok, Poland.
authors.forename: Agnieszka Z
authors.initials: AZ
authors.lastname: Wilczewska
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Wnorowska
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Piktel
authors.affiliation: Department of Hematology, Holy Cross Oncology Center of Kielce, Kielce, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Wątek
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
authors.forename: Paul B
authors.initials: PB
authors.lastname: Savage
authors.affiliation: Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Poland ; Department of Physiology, Pathophysiology and Microbiology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Kielce, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Bucki
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy*; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000627:therapeutic use; D013256:Steroids / Q000008:administration & dosage / Q000494:pharmacology; D000068936:Theranostic Nanomedicine / Q000379:methods; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D058185:Magnetite Nanoparticles; D013256:Steroids; C521528:ceragenin CSA-13; D054804:Cathelicidins
keywords: anticancer activity; cathelicidin LL-37; ceragenin; colorectal cancer; magnetic nanoparticles