title: Gata4, Tbx5 and Baf60c induce differentiation of adipose tissue-derived mesenchymal stem cells into beating cardiomyocytes.
abstract: The adipose tissue-derived mesenchymal stem cells (ADMSCs) are extensively utilized in tissue engineering, regenerative medicine and cell therapy. ADMSCs can differentiate into cardiomyocytes, and it has been shown that over-expression of a cocktail of factors can induce ectopic heart formation and program cardiogenesis in ESCs. However, which genes are responsible for differentiation of ADMSCs into beating cardiomyocyte-like cells remains unknown. In this study we have shown that the combination of Gata4, Tbx5 and Baf60c is sufficient for inducing ADMSCs to form cardiomyocytes. It also appears that, while Gata4 and Baf60c are key inducers of myocardial differentiation, Tbx5 is essential for the ability of cardiac cells to contract. These findings provide additional experimental references for myocardial tissue engineering in the emerging field of cell-based therapy of heart diseases.
authors.affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, PR China; Nursing school, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Li
authors.affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, PR China. Electronic address: gzk@xxmu.edu.cn.
authors.forename: Zhi-Kun
authors.initials: ZK
authors.lastname: Guo
authors.affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Yu-Qiao
authors.initials: YQ
authors.lastname: Chang
authors.affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yu
authors.affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Ci-Xia
authors.initials: CX
authors.lastname: Li
authors.affiliation: Department of Human Anatomy, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Li
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone; D050991:GATA4 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human; C491649:SMARCD3 protein, human; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5; D014157:Transcription Factors; D049452:Green Fluorescent Proteins
keywords: Adipose derived mesenchymal stem cells; Baf60c; Cardiomyocytes differentiation; Gata4; Tbx5; cTnT