title: Genome-derived cytosolic DNA contributes to type I interferon expression and immunogenicity of B-cell lymphoma cells.
abstract: We recently provided evidence that genome-derived DNA is present in the cytosol of many tumor cells. Genomic loci that give rise to cytosolic DNA can potentially form non-B DNA structures including triple-stranded RNA:DNA structures (R-loops). The RNA:DNA-specific endonuclease RNaseh1 reduced the levels of cytosolic DNA and type I interferon-dependent rejection of B-cell lymphoma suggesting that cytosolic DNA may contribute to immune surveillance of B-cell lymphoma.
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 117456, Singapore.
authors.forename: Yu J
authors.initials: YJ
authors.lastname: Shen
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore.
authors.forename: Samantha S W
authors.initials: SSW
authors.lastname: Ho
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore.
authors.forename: Nikki Y
authors.initials: NY
authors.lastname: Tan
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 117456, Singapore.
authors.forename: Christine Xing'Er
authors.initials: CX
authors.lastname: Koo
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore.
authors.forename: Muznah
authors.initials: M
authors.lastname: Khatoo
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore.
authors.forename: Florence S G
authors.initials: FSG
authors.lastname: Cheung
authors.affiliation: Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 117456, Singapore. Electronic address: micsg@nus.edu.sg.
authors.forename: Stephan
authors.initials: S
authors.lastname: Gasser
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics*; D016678:Genome; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000276:immunology*; D012313:RNA / Q000235:genetics
chemical_list: D007370:Interferon Type I; D012313:RNA; D004247:DNA
keywords: Cancer; DNA sensors; Innate immunity; Non-B DNA motifs; Type I interferon