title: Periodic monitoring of persistent organic pollutants and molecular damage in Cyprinus carpio from Büyük Menderes River.
abstract: Concentrations of persistent organic pollutants (POPs) were quantified in river water and sediment, as well as in the liver and muscle tissues of Cyprinus carpio that were sampled four times in a year at three stations in the Büyük Menderes River (BMR). Potential biomarkers of possible cellular molecular damage, namely lipid peroxidation (LPO) degradation products, protein carbonyls (PCO) and DNA repair product 8-hydroxy-2'-deoxyguanosine (8-OHdG), were analysed. All the targeted pollutants were measurable both in biotic and abiotic samples. Interestingly, the results suggested that there was recent organochlorine pesticide (OCP) input into the river water in the first two sampling periods in all stations in contrast to prohibition, while input of polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs) was not detected. Liver POP concentrations were higher than in muscle, as expected, and were found to decrease from the first to the fourth sampling period in all stations, except PBDEs. Levels of LPO degradation products in the liver and in muscle tissues decreased from the first to the fourth sampling period. This suggests that these markers reflect the lipid damage in respective tissues due to the tissue burden of targeted POPs. Protein carbonyls were the highest in the first sampling period, followed by a dramatic decrease in the second, and then a gradual increase towards the fourth sampling period in all stations. 8-OHdG levels were lower in Sarayköy station in the first sampling period. Among the measured biomarkers, only several LPO degradation products were significantly correlated with OCPs and PCBs in liver tissue.
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Ege University, 35100, Bornova-İzmir, Turkey.|Bioengineering Department, Faculty of Engineering, Kirikkale University, 71450, Merkez-Kirikkale, Turkey.
authors.forename: Beste
authors.initials: B
authors.lastname: Çağdaş
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Ege University, 35100, Bornova-İzmir, Turkey.
authors.forename: Rasih
authors.initials: R
authors.lastname: Kocagöz
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Ege University, 35100, Bornova-İzmir, Turkey.
authors.forename: İlgen
authors.initials: İ
authors.lastname: Onat
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ege University, 35100, Bornova-İzmir, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Perçin
authors.affiliation: Department of Hydrobiology, Faculty of Fisheries, Ege University, 35100, Bornova-İzmir, Turkey.
authors.forename: Okan
authors.initials: O
authors.lastname: Özaydın
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Ege University, 35100, Bornova-İzmir, Turkey. horhan@gmail.com.
authors.forename: Hilmi
authors.initials: H
authors.lastname: Orhan
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002347:Carps; D003849:Deoxyguanosine / Q000031:analogs & derivatives; D004784:Environmental Monitoring; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D014874:Water Pollutants, Chemical; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: Büyük Menderes River; Cyprinus carpio; DNA damage; Lipid peroxidation degradation products; POPs; Protein carbonyls