title: Molecular epidemiological characterization of poultry red mite, Dermanyssus gallinae, in Japan.
abstract: Dermanyssus gallinae, the poultry red mite, is an obligatory blood-sucking ectoparasite. The genetic diversity of D. gallinae has been examined in some countries, but so far not in Asian countries. Here, we sequenced a part of the mitochondrial cytochrome oxidase subunit I (COI) and16S rRNA genes and nuclear internal transcribed spacers (ITS) region in 239 mite samples collected from 40 prefectures throughout Japan. The COI and 16S rRNA nucleotide sequences were classified into 28 and 26 haplotypes, respectively. In phylogenetic trees, the haplotypes clustered into 2 haplogroups corresponding to haplogroups A and B, which were previously reported. Haplogroups A and B were further subdivided into sub-haplogroups AJ1 and AJ2, and BJ1 and BJ2, respectively. In both trees, the sequences of haplotypes in AJ1 and BJ2 were relatively distant from those reported in other countries, while some sequences in AJ2 and BJ1 were identical to those in Europe. In addition, the ITS sequences were classified into two sequences, and both sequences were closely related to the sequences found in European countries. These findings indicate a possibility of international oversea transmission of D. gallinae.
authors.affiliation: Laboratory of Veterinary Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama Minami, Tottori 680-8553, Japan.
authors.forename: Thi Thanh Huong
authors.initials: TT
authors.lastname: Chu
authors.forename: Takako
authors.initials: T
authors.lastname: Murano
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Uno
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Usui
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D002645:Chickens; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005060:Europe / Q000453:epidemiology; D007564:Japan / Q000453:epidemiology; D008924:Mite Infestations / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008925:Mites / Q000235:genetics*; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV