title: An Overview and History of Glyco-Engineering in Insect Expression Systems.
abstract: Insect systems, including the baculovirus-insect cell and Drosophila S2 cell systems are widely used as recombinant protein production platforms. Historically, however, no insect-based system has been able to produce glycoproteins with human-type glycans, which often influence the clinical efficacy of therapeutic glycoproteins and the overall structures and functions of other recombinant glycoprotein products. In addition, some insect cell systems produce N-glycans with immunogenic epitopes. Over the past 20 years, these problems have been addressed by efforts to glyco-engineer insect-based expression systems. These efforts have focused on introducing the capacity to produce complex-type, terminally sialylated N-glycans and eliminating the capacity to produce immunogenic N-glycans. Various glyco-engineering approaches have included genetically engineering insect cells, baculoviral vectors, and/or insects with heterologous genes encoding the enzymes required to produce various glycosyltransferases, sugars, nucleotide sugars, and nucleotide sugar transporters, as well as an enzyme that can deplete GDP-fucose. In this chapter, we present an overview and history of glyco-engineering in insect expression systems as a prelude to subsequent chapters, which will highlight various methods used for this purpose.
authors.affiliation: GlycoBac, LLC, Laramie, WY, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Geisler
authors.forename: Hideaki
authors.initials: H
authors.lastname: Mabashi-Asazuma
authors.forename: Donald L
authors.initials: DL
authors.lastname: Jarvis
mesh_terms: D000818:Animals; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006031:Glycosylation; D006801:Humans; D007313:Insecta / Q000235:genetics*; D011134:Polysaccharides / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides; D011994:Recombinant Proteins