title: Assessment of the cytotoxicity of a mineral trioxide aggregate-based sealer with respect to macrophage activity.
abstract: OBJECTIVE
  To assess the influence of co-culture with mineral trioxide aggregate (MTA) and MTA Fillapex (FLPX) on the viability, adherence, and phagocytosis activity of peritoneal macrophages from two mouse strains.
  
  
  METHODS
  Cellular viability, adherence, and phagocytosis of Saccharomyces boulardii were assayed in the presence of capillaries containing MTA and MTA Fillapex. The data were analyzed using parametric (Student's t) and non-parametric (Mann-Whitney) tests.
  
  
  RESULTS
  FLPX was severely cytotoxic and decreased cell viability, adherence, and phagocytic activity of both macrophage subtypes. Cells that were treated with MTA Fillapex remained viable (>80%) for only 4 h after stimulation. Macrophages from C57BL/6 mice presented higher adherence and higher phagocytic activity compared with macrophages from BALB/c mice.
  
  
  CONCLUSIONS
  Comparison of MTA and FLPX effects upon macrophages indicates that FLPX may impair macrophage activity and viability, while MTA seems to increase phagocytic activity.
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Julia Mourão
authors.initials: JM
authors.lastname: Braga
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Ricardo Reis
authors.initials: RR
authors.lastname: Oliveira
authors.affiliation: Departamento de Odontologia Social e Preventiva, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: de Castro Martins
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.|Nucleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, MG, Brazil.
authors.forename: Leda Quercia
authors.initials: LQ
authors.lastname: Vieira
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Antonio Paulino Ribeiro
authors.initials: AP
authors.lastname: Sobrinho
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004338:Drug Combinations; D008264:Macrophages / Q000187:drug effects*; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010087:Oxides / Q000633:toxicity*; D010587:Phagocytosis / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000633:toxicity*; D000069983:Saccharomyces boulardii; D017640:Silicates / Q000633:toxicity*
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate
keywords: Adherence; MTA Fillapex; Viability; macrophage; mineral trioxide aggregate; phagocytosis