title: [Determination of 2,4-dihydroxybenzophenone in mouse brain by high performance liquid chromatography].
abstract: OBJECTIVE
  To optimize and establish the experimental methods for the determination of 2,4-dihydroxybenzophenone (BP-1) in mouse brain.
  
  
  METHODS
  BP-1 was determined by high performance liquid chromatography (HPLC) and separated by Waters Symmetry C18 (4.6 mm×250 mm, 5 μm) using isocratic elution, and the sample preparation conditions were optimized by orthogonal experiment design. The mobile phase was methanol-water (volume ratio 3:1) containing 3% (volume fraction) acetic acid (pH 3.40) at a flow rate of 1.0 mL/min, and ultraviolet (UV) detection wavelength was set at 290 nm. Retention time was used for qualitative analysis and internal standard method for quantitative analysis.
  
  
  RESULTS
  Under the optimized experimental conditions, the calibration curve was linear with a correlation coefficient of 0.999 8 over the concentration range of 0.2-10.0 mg/L. The recoveries of BP-1 were between 96.8% and 104.5%. The intra-day and inter-day precision of BP-1 were 3.5%-5.7% and 4.5%-6.4%, respectively. The extraction recoveries of BP-1 at three concentrations (0.5, 2.0, 8.0 mg/L) in the mouse brain were 90.5%, 89.5%, and 97.7%, and the matrix effect of BP-1 at these three concentrations were 102.9%, 102.7%, and 90.9%, respectively.
  
  
  CONCLUSIONS
  The method is simple, accurate, and suitable for determination of the contents of BP-1 in mouse brain.
authors.affiliation: Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Yi-chao
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Cui
authors.affiliation: Department of Toxicology, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Yuan-li
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Toxicology, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Bao-xu
authors.initials: BX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001577:Benzophenones / Q000737:chemistry*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D051379:Mice
chemical_list: D001577:Benzophenones; C121479:2,4-dihydroxybenzophenone