title: A retrievable, water-soluble and biocompatible fluorescent probe for recognition of Cu(II) and sulfide based on a peptide receptor.
abstract: A novel fluorescent probe C-GGH for recognition of Cu(2+) and S(2-) was designed and synthesized by incorporation a natural tripeptide GGH (Gly-Gly-His) moiety, which acts as a recognition unit for Cu(2+), to a coumarin fluorophore. C-GGH displayed ON-OFF type fluorescence response towards Cu(2+) with high selectivity in 100% aqueous solution. Furthermore, the in situ generated non-fluorescent C-GGH-Cu(2+) complex can serve as an effective OFF-ON type fluorescent probe for selective sensing sulfide anion. Due to the strong binding affinity of S(2-) to Cu(2+), sulfide anion can extract Cu(2+) from C-GGH-Cu(2+) complex, resulting the release of C-GGH and the revival of fluorescent emission of the system. Techniques of emission spectrometry, NMR, mass spectrometry, and HPLC were used to confirm the displacement mechanism of C-GGH-Cu(2+) for sensing sulfide anion. The probe C-GGH-Cu(2+) allowed detection of S(2-) in aqueous buffer solution with a LOD (limit of detection) of 0.44μM. By taking advantages of the natural tripeptide GGH, the sensor exhibits excellent biocompatibility and water solubility. Moreover, the cell imaging study demonstrated that the probe can be exploited to detect sulfur anion in biological systems.
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, PR China.
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Hao
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Wansong
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, PR China. Electronic address: 3523805920@163.com.
authors.forename: Yintang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qu
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, PR China; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410111, PR China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China. Electronic address: liuyounian@sqnc.edu.cn.
authors.forename: You-Nian
authors.initials: YN
authors.lastname: Liu
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal Universtiy, Shangqiu 476000, PR China. Electronic address: xumaotian@sqnc.edu.cn.
authors.forename: Maotian
authors.initials: M
authors.lastname: Xu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D013440:Sulfides; D014867:Water; D003300:Copper
keywords: Copper ion; Fluorescent probe; Gly-Gly-His; Sulfide sensing