title: Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients.
abstract: A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA + csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia.
authors.forename: O E
authors.initials: OE
authors.lastname: Bryzgunova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia ; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Tamkovich
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Cherepanova
authors.affiliation: Meshalkin Novosibirsk State Research Institute of Circulation Pathology, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Yarmoshchuk
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Permyakova
authors.affiliation: Meshalkin Novosibirsk State Research Institute of Circulation Pathology, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
authors.forename: O Y
authors.initials: OY
authors.lastname: Anykeeva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrentieva, 8, 630090, Novosibirsk, Russia ; Meshalkin Novosibirsk State Research Institute of Circulation Pathology, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.
authors.forename: P P
authors.initials: PP
authors.lastname: Laktionov
keywords: DNase activity; circulating DNA; plasma; prostate cancer