title: Arbuscular mycorrhizal symbiosis and methyl jasmonate avoid the inhibition of root hydraulic conductivity caused by drought.
abstract: Hormonal regulation and symbiotic relationships provide benefits for plants to overcome stress conditions. The aim of this study was to elucidate the effects of exogenous methyl jasmonate (MeJA) application on root hydraulic conductivity (L) of Phaseolus vulgaris plants which established arbuscular mycorrhizal (AM) symbiosis under two water regimes (well-watered and drought conditions). The variation in endogenous contents of several hormones (MeJA, JA, abscisic acid (ABA), indol-3-acetic acid (IAA), salicylic acid (SA)) and the changes in aquaporin gene expression, protein abundance and phosphorylation state were analyzed. AM symbiosis decreased L under well-watered conditions, which was partially reverted by the MeJA treatment, apparently by a drop in root IAA contents. Also, AM symbiosis and MeJA prevented inhibition of L under drought conditions, most probably by a reduction in root SA contents. Additionally, the gene expression of two fungal aquaporins was upregulated under drought conditions, independently of the MeJA treatment. Plant aquaporin gene expression could not explain the behaviour of L. Conversely, evidence was found for the control of L by phosphorylation of aquaporins. Hence, MeJA addition modified the response of L to both AM symbiosis and drought, presumably by regulating the root contents of IAA and SA and the phosphorylation state of aquaporins.
authors.affiliation: Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), C/ Profesor Albareda 1, 18008, Granada, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sánchez-Romera
authors.affiliation: Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), C/ Profesor Albareda 1, 18008, Granada, Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Ruiz-Lozano
authors.affiliation: CIPAV TimacAGRO International-Roullier Group, Polígono Arazuri-Orcoyen, c/C no. 32, 31160, Orcoyen, Navarra, Spain.
authors.forename: Ángel María
authors.initials: ÁM
authors.lastname: Zamarreño
authors.affiliation: CIPAV TimacAGRO International-Roullier Group, Polígono Arazuri-Orcoyen, c/C no. 32, 31160, Orcoyen, Navarra, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: García-Mina
authors.affiliation: Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), C/ Profesor Albareda 1, 18008, Granada, Spain. raroca@eez.csic.es.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Aroca
mesh_terms: D000085:Acetates / Q000378:metabolism*; D020346:Aquaporins / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D055864:Droughts; D038821:Mycorrhizae / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism*; D027805:Phaseolus / Q000382:microbiology* / Q000502:physiology; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology* / Q000502:physiology; D013312:Stress, Physiological; D013559:Symbiosis
chemical_list: D000085:Acetates; D020346:Aquaporins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C072239:methyl jasmonate
keywords: Aquaporins; Arbuscular mycorrhiza; Drought; Methyl jasmonate; Root hydraulic conductivity