title: New Activities for Isolated Compounds from Convolvulus austro-aegyptiacus as Anti-ulcerogenic, Anti-Helicobacter pylori and Their Mimic Synthesis Using Bio-guided Fractionation.
abstract: Bio-guided fractionation of the total alcoholic extract of Convolvulus austro-aegyptiacus was screened for its anti-ulcerogenic activity, using an absolute-ethanol-induced ulcer model at 500 and 1000 mg/kg doses. Two compounds were isolated from the butanol extract of C. austro-aegyptiacus and identified by1H and13C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50 mg/kg) showed a remarkable anti-ulcerogenic activity because they exhibited control-ulcer protection by 16.7% and 90.8%, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD50)was more than 4000 mg/kg. Moreover, the obtained results were confirmed by the sub-chronic toxicity because the rats that have been administered 1000 mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Pharmacognosy Department, College of Pharmacy, Salman bin Abdulaziz University, Al-Kharj, KSA.
authors.forename: Amani S
authors.initials: AS
authors.lastname: Awaad
authors.affiliation: Chemistry Department, College of Science, King Saud University, Riyadh, KSA.
authors.forename: Asmaa A
authors.initials: AA
authors.lastname: Al-Rifai
authors.affiliation: Aromatic and Medicinal Plant Dept., Desert Research Centre, Cairo, Egypt.
authors.forename: Reham M
authors.initials: RM
authors.lastname: El-Meligy
authors.affiliation: Medicinal Chemistry Department, College of Pharmacy, Salman bin Abdulaziz University, Al-Kharj, KSA.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Alafeefy
authors.affiliation: Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Zain
keywords: H. pylori; cytoprotective; liver and kidney functions; peptic ulcer; scopoletin; scopolin