title: Ultra-large field-of-view two-photon microscopy.
abstract: We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of the microscope by recording resting-state vasomotion across both hemispheres of the murine brain through a transcranial window and by imaging histological sections without the need to stitch.
authors.forename: Philbert S
authors.initials: PS
authors.lastname: Tsai
authors.forename: Celine
authors.initials: C
authors.lastname: Mateo
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Field
authors.forename: Chris B
authors.initials: CB
authors.lastname: Schaffer
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Anderson
authors.forename: David
authors.initials: D
authors.lastname: Kleinfeld
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply; D004867:Equipment Design; D021621:Imaging, Three-Dimensional; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D055620:Optical Phenomena; D014666:Vasomotor System / Q000502:physiology