title: Potential therapeutic agents for circulatory diseases from Bauhinia glauca Benth.subsp. pernervosa. (Da Ye Guan Men).
abstract: Because of platelets as critical factor in the formation of pathogenic thrombi, anti-platelet activities have been selected as therapeutic target for various circulatory diseases. In order to find potential therapeutic agents, bioassay-directed separation of Bauhinia glauca Benth.subsp. pernervosa. (called Da Ye Guan Men as a traditional Chinese medicine) was performed to get 29 main components (compounds 1-29) from the bioactive part of this herbal. It was the first time to focus on the composition with anti-platelet aggregation activities for this traditional Chinese medicine. The constituents, characterized from the effective extract, were established on the basis of extensive spectral data analysis. Then their anti-platelet aggregation effects were evaluated systematically. On the basis of the chemical profile and biological assay, it was suggested that the flavonoid composition (5 and 18) should be responsible for the anti-platelet aggregation of the herbal because of their significant activities. The primary structure and activity relationship was also discussed briefly.
authors.affiliation: School of Traditional Chinese Materia Medica 49(#), Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Yingzhan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Ling
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Materia Medica 49(#), Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Materia Medica 49(#), Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Materia Medica 49(#), Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Materia Medica 49(#), Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China. Electronic address: liningsypharm@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D001792:Blood Platelets / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors
keywords: Bauhinia glauca Benth.subsp. pernervosa.; Bioassay-directed separation; Effective flavonoid composition; Platelet aggregation inhibiting activities; Structure and activity relationship