title: Hypergravity-induced enrichment of β1 integrin on the cell membranes of osteoblast-like cells via caveolae-dependent endocytosis.
abstract: In bone cells, integrins on the cellular surface are the primary sensors of their mechanical environment. Although gravitational changes are known to affect the adhesion and functions of bone cells, whether integrins respond to hypergravity in osteoblasts remains unclear. In this work, we demonstrate that exposure to a hypergravitational environment (20 × g via centrifugation) resulted in the concentration of β1, but not β3, integrin on the cell membrane of osteoblast-like (MC3T3-E1) cells. Notably, the total expression of both integrins was unaffected by the hypergravitational environment. In addition, caveolin-dependent endocytosis was discovered to be involved in the regulation of the enrichment of β1 integrin on the cell surface after stimulation by hypergravity. These findings could aid in the improvement of our understanding of the mechanisms underlying the effects of different gravitational forces on the human body.
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Institute of Biomechanics and Medical Engineering, School of Aerospace, Tsinghua University, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zu
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Zhuang
authors.affiliation: Institute of Biomechanics and Medical Engineering, School of Aerospace, Tsinghua University, Beijing, China. Electronic address: yangchun@mail.tsinghua.edu.cn.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D021941:Caveolae / Q000502:physiology*; D017931:DNA Primers; D004705:Endocytosis; D018471:Hypergravity; D019012:Integrin beta1 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D019012:Integrin beta1; D039661:Integrin beta3
keywords: Caveolae; Gravity sensors; Hypergravity; Integrin; Mechanotransduction; Osteoblasts