title: [Buyang Huanwu decoction promotes neuroblast migration from subventricular zone via inducing angiogenesis after ischemia].
abstract: OBJECTIVE
  To study the effect of Buyang Huanwu decoction (BYHWD) inducing angiogenesis on the neuroblast migration from the subventricular zone and its mechanisms after focal cerebral ischemia.
  
  
  METHODS
  The middle cerebral artery occlusion (MCAO) was performed to mice for 30 minutes to establish the model. The rats were divided into sham group, model group, BYHWD group and endostatin group. BYHWD (20 g x kg(-1), ig) and endostatin (10 μg, sc) were administered 24 h after ischemia once a day for consecutively 14 days. At 14 d after ischemia, the density of micro-vessel and the number of neuroblasts in the ischemia border zone were determined by immunofluorescence staining. The mRNA and protein expression of cell-derived factor-1 (SDF-1) and brain-derived neurotrophic (BDNF) were examined by real-time PCR and Western blot.
  
  
  RESULTS
  Compared with the model group, BYHWD significantly increased the density of micro-vessel and the number of DCX positive cells in the ischemia border zone (P < 0.01), and significantly increased the SDF-1 and BDNF mRNA and protein expression (P < 0.01). Compared with BYHWD group, endostatin significantly reduced the density of micro-vessel and the number of DCX positive cells in the ischemia border zone (P < 0.01), as well as the SDF-1, BDNF mRNA and protein expression (P < 0.01).
  
  
  CONCLUSIONS
  BYHWD could promote the neuroblast migration from the subventricular zone via inducing angiogenesis after cerebral ischemia, the mechanism may be correlated with up-regulating the expression of SDF-1 and BDNF.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Zhi-ting
authors.initials: ZT
authors.lastname: Liu
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Chu
authors.forename: Tian-hong
authors.initials: TH
authors.lastname: Yu
authors.forename: Tie-bing
authors.initials: TB
authors.lastname: Qu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Cui-cui
authors.initials: CC
authors.lastname: Ren
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects*; D002552:Cerebral Ventricles / Q000473:pathology*; D054377:Chemokine CXCL12 / Q000032:analysis / Q000235:genetics; D000089662:Doublecortin Protein; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000187:drug effects* / Q000502:physiology
chemical_list: D043925:Angiogenesis Inducing Agents; D019208:Brain-Derived Neurotrophic Factor; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D004365:Drugs, Chinese Herbal; C460102:buyang huanwu