title: Endogenous neural stem cells in central canal of adult rats acquired limited ability to differentiate into neurons following mild spinal cord injury.
abstract: Endogenous neural stem cells in central canal of adult mammalian spinal cord exhibit stem cell properties following injury. In the present study, the endogenous neural stem cells were labeled with Dil to track the differentiation of cells after mild spinal cord injury (SCI). Compared with 1 and 14 days post mild injury, the number of endogenous neural stem cells significantly increased at the injured site of spinal cord on 3 and 7 days post-injury. Dil-labeled βIII-tublin and GFAP expressing cells could be detected on 7 days post-injury, which indicated that the endogenous neural stem cells in central canal of spinal cord differentiated into different type of neural cells, but there were more differentiated astrocytes than the neurons after injury. Furthermore, after injury the expression of inhibitory Notch1 and Hes1 mRNA began to increase at 6 hours and was evident at 12 and 24 hours, which maintained high levels up to 7 days post-injury. These results indicated that a mild SCI in rat is sufficient to induce endogenous neural stem cells proliferation and differentiation. However, the ability to differentiate into neurons is limited, which may be, at least in part, due to high expression of inhibitory Notch1 and Hes1 genes after injury.
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Botao
authors.initials: B
authors.lastname: Tan
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Zaiyun
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D005260:Female; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000473:pathology*
keywords: Endogenous neural stem cells; differentiation; proliferation; spinal cord injury