title: ABCG5 and ABCG8 gene polymorphisms in type 2 diabetes mellitus in the Turkish population.
abstract: OBJECTIVE
  The aim of the present study was to investigate the relationship between ABCG5 and ABCG8 gene polymorphisms and plasma lipid concentrations in Turkish patients with type 2 diabetes mellitus.
  
  
  METHODS
  Included in this study were 80 patients with type 2 diabetes and 73 healthy controls. Two selected single nucleotide polymorphisms in ABC transporter genes, ABCG5 (rs6720173) and ABCG8 (rs4148211), were genotyped by using the polymerase chain reaction-restriction fragment length polymorphism technique.
  
  
  RESULTS
  The rate of having the ABCG8 AA genotype (p=0.001) was significantly higher in the patients than in the control subjects. Correspondingly, the rates of having the AG genotype (p=0.001) and the G allele (p=0.001) were significantly lower in the patients than in controls. Upon comparing the groups regarding ABCG5, the frequencies of occurrence of the GG genotype (p=0.031) and G allele (p=0.003) were considerably higher in patients than in control subjects. In the patients, the rates of having the CC genotype (p=0.003) and the C allele (p=0.031) were also significantly lower than those in control subjects. There was no significant difference between G5 and G8 polymorphism and lipid levels in the study groups. The ABCG8 AA genotype carriers had higher triglyceride (p=0.045) and very low-density-cholesterol (p=0.045) levels than the ABCG8 GG genotype carriers in all study populations.
  
  
  CONCLUSIONS
  These results indicate that the AA genotype for ABCG8 and the GG genotype and G allele for ABCG5 are risk factors for diabetes. This study reveals the first data concerning the ABCG5 and ABCG8 gene polymorphisms in Turkish patients with diabetes.
authors.affiliation: Department of Molecular Medicine, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey; Department of Medical Biology and Regenerative and Restorative Medicine Research Center (REMER), Faculty of Medicine, Istanbul Medipol University, Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Gok
authors.affiliation: Department of Internal Medicine, Haseki Training and Research Hospital, Istanbul, Turkey.
authors.forename: Zeynep Ermis
authors.initials: ZE
authors.lastname: Karaali
authors.affiliation: Department of Molecular Medicine, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Acar
authors.affiliation: Department of Medical Biology and Regenerative and Restorative Medicine Research Center (REMER), Faculty of Medicine, Istanbul Medipol University, Istanbul, Turkey.
authors.forename: Ulkan
authors.initials: U
authors.lastname: Kilic
authors.affiliation: Department of Molecular Medicine, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey. Electronic address: harzue@gmail.com.
authors.forename: Arzu
authors.initials: A
authors.lastname: Ergen
mesh_terms: D000071018:ATP Binding Cassette Transporter, Subfamily G, Member 5; D000071019:ATP Binding Cassette Transporter, Subfamily G, Member 8; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014421:Turkey
chemical_list: C416945:ABCG5 protein, human; C416946:ABCG8 protein, human; D000071018:ATP Binding Cassette Transporter, Subfamily G, Member 5; D000071019:ATP Binding Cassette Transporter, Subfamily G, Member 8; D018528:ATP-Binding Cassette Transporters; D008055:Lipids; D008074:Lipoproteins
keywords: ABCG5; ABCG8; diabetes mellitus; diabète sucré; lipid metabolism; métabolisme des lipides; polymorphism; polymorphisme