title: [Anti-metastasis of celastrol on esophageal cancer cells and its mechanism].
abstract: Celastrol is a quinone methyl terpene extracted from the traditional Chinese medicine Tripterygium wilfordii, which has anti-inflammatory, immune suppression and pharmacological activities, as well as anti-tumor activity. The effects of celastrol on adhesion, migration and invasion of esophageal cancer cells were investigated in this experiment. Human esophageal cancer cell line ECA-109 was used. The inhibition of ECA-109 cells' adhesion induced by celastrol was measured by MTT test. The inhibition of ECA-109 cells' migration induced by celastrol was measured by scratch test. The invasion inhibition of ECA-109 cells induced by celastrol was measured by Transwell experiment. Quantitative real-time PCR and Western blot were used to determine the effects of different concentrations of celastrol on integrin family and Wnt signaling pathway in ECA-109 cells. The results showed that celastrol inhibited adhesion, migration and invasion of ECA-109 cells and expressions of integrins β1, β4, αv and β-Catenin, LRP6 in Wnt signal pathway in a dose-dependent manner. Therefore the study suggests that celastrol could inhibit the cell metastasis of esophageal cancer by inhibiting the Wnt signaling pathway and the expressions of integrins.
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong 637009, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong 637009, China. wcl_xj@163.com.|State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, Southwest University, Chongqing 400715, China.
authors.forename: Chun-Lian
authors.initials: CL
authors.lastname: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D053978:Pentacyclic Triterpenes; D060888:Real-Time Polymerase Chain Reaction; D014315:Triterpenes / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C050414:celastrol