title: A comparative proteomic study of plasma in feline pancreatitis and pancreatic carcinoma using 2-dimensional gel electrophoresis to identify diagnostic biomarkers: A pilot study.
abstract: While pancreatitis is now recognized as a common ailment in cats, the diagnosis remains challenging due to discordant results and suboptimal sensitivity of ultrasound and specific feline pancreatic lipase (Spec fPL) assay. Pancreatitis also shares similar clinical features with pancreatic carcinoma, a rare but aggressive disease with a grave prognosis. The objective of this pilot study was to compare the plasma proteomes of normal healthy cats (n = 6), cats with pancreatitis (n = 6), and cats with pancreatic carcinoma (n = 6) in order to identify potential new biomarkers of feline pancreatic disease. After plasma protein separation by 2-dimensional gel electrophoresis, protein spots were detected by Coomassie Brilliant Blue G-250 staining and identified by mass spectrometry. Alpha-1-acid glycoprotein (AGP), apolipoprotein-A1 (Apo-A1), and apolipoprotein-A1 precursor (Pre Apo-A1) appeared to be differentially expressed, which suggests the presence of a systemic acute-phase response and alteration of lipid metabolism in cats with pancreatic disease. Future studies involving greater case numbers are needed in order to assess the utility of these proteins as potential biomarkers. More sensitive proteomic techniques may also be helpful in detecting significant but low-abundance proteins.
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Melissa D
authors.initials: MD
authors.lastname: Meachem
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Elisabeth R
authors.initials: ER
authors.lastname: Snead
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Beverly A
authors.initials: BA
authors.lastname: Kidney
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Marion L
authors.initials: ML
authors.lastname: Jackson
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Ryan
authors.initials: R
authors.lastname: Dickinson
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Victoria
authors.initials: V
authors.lastname: Larson
authors.affiliation: Department of Veterinary Pathology (Meachem, Kidney, Jackson, Simko) and Department of Small Animal Clinical Sciences (Snead), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Prairie Diagnostic Services, Saskatoon, Saskatchewan S7N 5B4 (Dickinson); Calgary Animal Referral and Emergency Centre, Calgary, Alberta T2H 2Y4 (Larson).
authors.forename: Elemir
authors.initials: E
authors.lastname: Simko
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002371:Cat Diseases; D002415:Cats; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000662:veterinary*; D010190:Pancreatic Neoplasms / Q000097:blood / Q000662:veterinary*; D010195:Pancreatitis / Q000097:blood / Q000662:veterinary*; D010865:Pilot Projects; D040901:Proteomics
chemical_list: D015415:Biomarkers