title: The effects of narrow-band middle infrared radiation in enhancing the antitumor activity of paclitaxel.
abstract: Paclitaxel is used as an adjuvant to enhance the effectiveness of ionization radiation therapy; however, high-energy radiation often damages the healthy cells surrounding cancer cells. Low-energy, middle-infrared radiation (MIR) has been shown to prevent tissue damage, and recent studies have begun combining MIR with paclitaxel. However, the cytotoxic effects of this treatment combination remain unclear, and the mechanism underlying its effects on HeLa cells has yet to be elucidated. This study investigated the effectiveness of treating HeLa human cervical cancer cells with a combination of paclitaxel for 48 h in conjunction with narrow-band MIR from 3.0 to 5.0 μm. This combined treatment significantly inhibited the growth of HeLa cells. Specifically, results from Annexin V-FITC/PI apoptosis detection and cell mitochondrial membrane potential analyses revealed an increase in apoptotic cell death and a collapse of mitochondrial membrane potential. One possible mechanism underlying cellular apoptosis is an increase in oxidative stress. These preliminary findings provide evidence to support the combination of narrow-band MIR with paclitaxel as an alternative approach in the treatment of human cervical cancer.
authors.affiliation: a Graduate Institute of Biomedical Electronics and Bioinformatics , National Taiwan University , Taipei , Taiwan.
authors.forename: Shang-Ru
authors.initials: SR
authors.lastname: Tsai
authors.affiliation: b Department of Obstetrics & Gynecology , National Taiwan University , Taipei , Taiwan.
authors.forename: Bor-Ching
authors.initials: BC
authors.lastname: Sheu
authors.affiliation: b Department of Obstetrics & Gynecology , National Taiwan University , Taipei , Taiwan.
authors.forename: Pei-Shen
authors.initials: PS
authors.lastname: Huang
authors.affiliation: a Graduate Institute of Biomedical Electronics and Bioinformatics , National Taiwan University , Taipei , Taiwan.|c Department of Electrical Engineering , National Taiwan University , Taipei , Taiwan.
authors.forename: Si-Chen
authors.initials: SC
authors.lastname: Lee
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D017239:Paclitaxel / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D017239:Paclitaxel
keywords: HeLa cell; middle infrared radiation; mitochondrial membrane potential; paclitaxel