title: Quantitative analysis of differential protein expression in cervical carcinoma cells after zeylenone treatment by stable isotope labeling with amino acids in cell culture.
abstract: Cervical carcinoma is a malignant tumor that poses a serious threat to women's health and survival. Approximately 10-25% of cervical cancers are adenocarcinomas (ACs). AC has high rates of recurrence and mortality, while there is no effective treatment for now. Zeylenone (Zey), which is isolated from an ethanol extract of the leaves of Uvaria grandiflora Roxb. of the family Annonaceae, has shown potent inhibitory activity against various tumor cells, including cervical carcinoma cells. To gain insight into the molecular mechanism underlying the effect of Zey on AC, we quantified protein expression changes in AC cells treated with Zey. We used stable isotope labeling with amino acids in cell culture (SILAC) in combination with high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) and bioinformatics analysis to compare protein expression profiles in HeLa cells before and after Zey treatment. Of 1805 differentially expressed proteins identified, 229 were screened as key protein molecules and classified into nine categories. Profiling of differentially-expressed proteins contributed to our understanding of the molecular mechanism by which Zey induces HeLa cell apoptosis. Using this method, candidate targets can be identified for developing new drugs against cervical carcinoma.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China. Electronic address: leining1129@163.com.
authors.forename: Leilei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Hu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China. Electronic address: lcao@implad.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Huo
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Dongyu
authors.initials: D
authors.lastname: Liu
authors.affiliation: Xinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ma
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; Yun Nan University of Traditional Chinese Medicine, Kunming 650500, China.
authors.forename: Canhong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Han
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Liyong
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China. Electronic address: sun_xiaobo163@163.com.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D003510:Cyclohexanes / Q000494:pharmacology*; D004146:Dioxanes / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D040901:Proteomics / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003510:Cyclohexanes; D004146:Dioxanes; D009363:Neoplasm Proteins; C516240:zeylenone
keywords: Adenocarcinomas; Apoptosis; Cervical carcinoma; Stable isotope labeling with amino acids in cell culture (SILAC); Tandem mass spectrometry; Zeylenone