title: [An intriguing model for 5S rDNA sequences dispersion in the genome of freshwater stingray Potamotrygon motoro (Chondrichthyes: Potamotrygonidae)].
abstract: 5S rDNA genes of the stingray Potamotrygon motoro were PCR replicated, purified, cloned and sequenced. Two distinct classes of segments of different sizes were obtained. The smallest, with 342 bp units, was classified as class I, and the largest, with 1900 bp units, was designated as class II. Alignment with the consensus sequences for both classes showed changes in a few bases in the 5S rDNA genes. TATA-like sequences were detected in the nontranscribed spacer (NTS) regions of class I and a microsatellite (GCT) 10 sequence was detected in the NTS region of class II. The results obtained can help to understand the molecular organization of ribosomal genes and the mechanism of gene dispersion.
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista - UNESP, campus de Botucatu, 18618-000 Botucatu, SP, Brasil.|cruzvp@outlook.com.
authors.forename: V P
authors.initials: VP
authors.lastname: Cruz
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista - UNESP, campus de Botucatu, 18618-000 Botucatu, SP, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Oliveira
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista - UNESP, campus de Botucatu, 18618-000 Botucatu, SP, Brasil.
authors.forename: F
authors.initials: F
authors.lastname: Foresti
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D021901:DNA, Intergenic; D020459:Genes, rRNA; D016678:Genome; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012860:Skates, Fish / Q000235:genetics*
chemical_list: D021901:DNA, Intergenic; D012341:RNA, Ribosomal, 5S
keywords: 5S rDNA; Elasmobranchii; Potamotrygon; clone; microsatellite; stingray