title: Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis.
abstract: Carrageenans are sulfated galactan isolated from marine red algae with different disaccharide forms. There are also some hybrid carrageenan-like oligomers, which are reported to possess a number of bioactivities. Here, we describe a method to study the structural characterization of a carrageenan-like sulfated galactan FB1 extracted from the red seaweed Furcellaria lumbricalis. We show the process of the general analysis of FB1, including the molecular weight, sulfate content, total sugar content, protein content, and 3,6-anhydrogalactose (3,6-AnG) content analyses. The fine structure identification methods, including desulfation and methylation, nuclear magnetic resonance (NMR), and electrospray ionization collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS), are also described in detail.
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, 5 Yu Shan Rd., Qingdao, 266003, P.R. China.
authors.forename: Youjing
authors.initials: Y
authors.lastname: Lv
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Junzeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Guangli
authors.initials: G
authors.lastname: Yu
mesh_terms: D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives / Q000032:analysis; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D000461:Rhodophyta / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013431:Sulfates / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005685:Galactans; D013431:Sulfates; C117625:3,6-anhydrogalactose; D002351:Carrageenan; D005690:Galactose