title: Effect of Matrine on HPAC cell migration by down-regulating the expression of MT1-MMP via Wnt signaling.
abstract: OBJECTIVE
  This study sought to explore the exact mechanism of Matrine inhibited migration and invasion of human pancreatic cancer cells.
  
  
  METHODS
  HPAC or Capan-1 cells were cultured in completed RPMI-1640 medium, contained with 50 μg/ml Matrine or 0.05 μg/ml docetaxel, respectively. Cell viability was evaluated by spectrophotometric analysis using MTT assay. Wound healing assay and transwell approach were used to detect the effects of Matrine on HPAC cell migration and invasion. Western Blot and RT-PCR were performed to detect the expressions of MT1-MMP, Wnt and β-Catenin. CHIP assay was used to detect whether the MT1-MMP transcription activity correlated with Wnt signaling pathway.
  
  
  RESULTS
  MTT results indicated that cell proliferration was inhibited by Matrine at a range of concentrations, especially at high dose. We further found that Matrine treatment significantly induced cell migration and invasion decreased. Interestingly, the expression of MT1-MMP decreased evidently upon Matrine treatment, paralleled with the expressions of Wnt and β-Catenin detected by Western Blot and RT-PCR assay. Further analysis of MT1-MMP transcription activity revealed that Matrine reduced the expression of MT1-MMP mediated by Wnt signaling pathway.
  
  
  CONCLUSIONS
  Matrine play a vital role in inhibiting HPAC cellular migration and invasion through down-regulating the expression of MT1-MMP via Wnt signaling pathway.
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.|Key lab, of BioMedicine of Luohe City, Luohe, China.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.|Key lab, of BioMedicine of Luohe City, Luohe, China.
authors.forename: Fazhang
authors.initials: F
authors.lastname: Zou
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Junping
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wan
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Yin
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Xianjia
authors.initials: X
authors.lastname: Li
authors.affiliation: Chengde Nursing Vocational College, Chengde, China.
authors.forename: Zhanyu
authors.initials: Z
authors.lastname: Bei
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Meng
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Luohe Medical College, Daxue Road, #148, Luohe City, Henan Province 462000 People's Republic of China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Song
keywords: Cell migration; Human pancreatic cancer; MT1-MMP; Matrine; Wnt