title: [Exploration of the role of cisplatin on transformation of larvngeal tumor cells to stem-like cancer cells].
abstract: OBJECTIVE
  To explore the possibility mechanism of non-side population cells (NSP) of Hep-2 be induced into stem-like cancer cells by chemotherapy drug--cisplatin.
  
  
  METHODS
  Hep-2 cell lines were sorted by fluorescence-actived cell sorting. The acquired NSP cells in trail group were co-cultured with cisplatin for more than 48 hours,while the control group with normal saline(NS). Then identified the percentage of the side population (SP) cells by flow cytometer. The β-catenin, notch-1 mRNA in trial and control group were detected using quantitative realtime PCR, and the β-catenin, notch-1 protein in two groups were compared by Western blot.
  
  
  RESULTS
  The percentage of side population cells in two groups were (17.16 ± 0.18)%, (10.05 ± 1.20)%, respectively. There was significant difference between two groups (t = 5.844, P < 0.01). The expression of β-catenin, notch-1 was higher in trail group by qRT-PCR; the protein levels of β- catenin, notch-1 was found to inceased in the trail group by Western blot (t = 5.155, P = 0.031; t = 5.977, P = 0.004). Statistical analysis showed significant difference between two groups (P < 0.05).
  
  
  CONCLUSIONS
  NSP cells can be differentiated into stem-like cancer cells after being treating with cisplatin. The supposed mechanism is maybe through wnt/β-catenin, notch signaling transduction pathway abnormalities.
authors.forename: Maomao
authors.initials: M
authors.lastname: Ai
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002469:Cell Separation; D002945:Cisplatin / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D012333:RNA, Messenger; D015398:Signal Transduction; D051176:beta Catenin
chemical_list: D000970:Antineoplastic Agents; D012333:RNA, Messenger; D051176:beta Catenin; D002945:Cisplatin