title: The Patterns of Coevolution in Clade B HIV Envelope's N-Glycosylation Sites.
abstract: The co-evolution of the potential N-glycosylation sites of HIV Clade B gp120 was mapped onto the coevolution network of the protein structure using mean field direct coupling analysis (mfDCA). This was possible for 327 positions with suitable entropy and gap content. Indications of pressure to preserve the evolving glycan shield are seen as well as strong dependencies between the majority of the potential N-glycosylation sites and the rest of the structure. These findings indicate that although mainly an adaptation against antibody neutralization, the evolving glycan shield is structurally related to the core polypeptide, which, thus, is also under pressure to reflect the changes in the N-glycosylation. The map we propose fills the gap in previous attempts to tease out sequon evolution by providing a more general molecular context. Thus, it will help design strategies guiding HIV gp120 evolution in a rational way.
authors.affiliation: University of Michigan Health System, Ann Arbor, MI, United States of America.
authors.forename: Swetha
authors.initials: S
authors.lastname: Garimalla
authors.affiliation: Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kieber-Emmons
authors.affiliation: Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America; Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria; UMRS872(equip 16), INSERM UMRS 872 Centre de Recherche des Cordeliers, Paris, France.
authors.forename: Anastas D
authors.initials: AD
authors.lastname: Pashov
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D019143:Evolution, Molecular; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D010802:Phylogeny
chemical_list: D015699:HIV Envelope Protein gp120