title: Sleep Disturbance and Altered Expression of Circadian Clock Genes in Patients With Sudden Sensorineural Hearing Loss.
abstract: The cause of sudden sensorineural hearing loss (SSNHL) remains unclear and therefore it is often considered as idiopathic. Sleep disturbance has been linked to SSNHL and circadian rhythm disruption, but the link between circadian rhythm disruption and SSNHL has never been investigated.In this study, we surveyed the sleep quality of 38 patients with SSNHL using a simple insomnia sleep questionnaire. The expression of circadian clock genes in peripheral blood (PB) leukocytes from 38 patients with SSNHL and 71 healthy subjects was accessed using real-time quantitative reverse transcriptase-polymerase chain reaction and validated using immunocytochemical staining.We found that 61.8% of patients with SSNHL suffered from insomnia before the insult of hearing loss. Besides, significantly decreased expression of PER1, CRY1, CRY2, CLOCK, BMAL1, and CKlε was found in PB leukocytes of patients with SSNHL when compared with healthy subjects. SSNHL patients with vertigo had significantly lower expression of CRY1 and CKlε than patients without vertigo symptoms. Our results imply the association of sleep disturbance and disrupted circadian rhythm in SSNHL.
authors.affiliation: From Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan (CHY, JHC, CMH, CFH); Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan (CHY, JHC, CMH, MYY); Department of Nursing, I-Shou University, Kaohsiung, Taiwan (PML); Division of Hematology-Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan (SFL); and Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (SFL).
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Yang
authors.forename: Chung-Feng
authors.initials: CF
authors.lastname: Hwang
authors.forename: Pai-Mei
authors.initials: PM
authors.lastname: Lin
authors.forename: Jiin-Haur
authors.initials: JH
authors.lastname: Chuang
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Hsu
authors.forename: Sheng-Fung
authors.initials: SF
authors.lastname: Lin
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D057906:Circadian Clocks / Q000235:genetics; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006319:Hearing Loss, Sensorineural / Q000209:etiology* / Q000378:metabolism; D003639:Hearing Loss, Sudden / Q000209:etiology* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012893:Sleep Wake Disorders / Q000150:complications*; D011795:Surveys and Questionnaires; D014717:Vertigo / Q000378:metabolism
chemical_list: D056925:Circadian Rhythm Signaling Peptides and Proteins