title: Quantification of Bufadienolides in Bryophyllum pinnatum Leaves and Manufactured Products by UHPLC-ESIMS/MS.
abstract: A quantitative assay for determination of the main bufadienolides bersaldegenin-1-acetate (1), bersaldegenin-3-acetate (2), bryophyllin A (3), and bersaldegenin-1,3,5-orthoacetate (4) in Bryophyllum pinnatum leaves and manufactured products was developed and validated. The assay involved extraction by pressurised liquid extraction, followed by quantification by ultrahigh performance liquid chromatography-tandem mass spectroscopy. The ultrahigh performance liquid chromatography-tandem mass spectroscopy method was applied to various batches of leaves harvested on several dates from plants grown at two locations (Brazil and Germany). In addition, press juices prepared from plants cultivated in Germany and Brazil were analysed. The total bufadienolide content ranged from 16.28 to 40.50 mg/100 g dry weight in leaves from plants grown in Brazil. The total content of these four bufadienolides was significantly lower in plants cultivated in Germany (3.78-12.49 mg/100 g dry weight, resp.). The total amounts of bufadienolides were 0.091-0.163 mg/100 mL and 0.89-1.16 mg/100 mL in press juices obtained from plants cultivated in Germany and Brazil, respectively. When analysing single leaves from individual plants, the content of bufadienolides was markedly higher in young leaves. For comparative purposes, the content of these bufadienolides was also determined in Bryophyllum daigremontianum and Bryophyllum tubiflorum. Bersaldegenin-1,3,5-orthoacetate (4) was predominant in the leaves of B. daigremontianum and in the stems of B. tubiflorum, while the leaves of B. tubiflorum contained very low amounts of 1-4.
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Mouhssin
authors.initials: M
authors.lastname: Oufir
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Christina
authors.initials: C
authors.lastname: Seiler
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Manon
authors.initials: M
authors.lastname: Gerodetti
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Julia
authors.initials: J
authors.lastname: Gerber
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Karin
authors.initials: K
authors.lastname: Fürer
authors.affiliation: Weleda AG, Arlesheim, Switzerland.
authors.forename: Monica
authors.initials: M
authors.lastname: Mennet-von Eiff
authors.affiliation: Research Department, Klinik Arlesheim AG, Arlesheim, Switzerland.
authors.forename: Siegward-M
authors.initials: SM
authors.lastname: Elsas
authors.affiliation: Department of Clinical Research, University of Bern, Bern, Switzerland.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Brenneisen
authors.affiliation: Department of Obstetrics, University Hospital Zurich, Zürich, Switzerland.
authors.forename: Ursula
authors.initials: U
authors.lastname: von Mandach
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
authors.affiliation: Department of Pharmaceutical Sciences, Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Olivier
authors.initials: O
authors.lastname: Potterat
mesh_terms: D001938:Brazil; D002018:Bufanolides / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005858:Germany; D031962:Kalanchoe / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002018:Bufanolides; C087925:bufadienolide