title: Rapid and simple colorimetric detection of Escherichia coli O157:H7 in apple juice using a novel recombinant bacteriophage-based Method.
abstract: In this study, a bacteriophage-based method for the colorimetric detection of E. coli O157:H7 in apple juice was investigated. Firstly, a gene encoding Cytochrome c Peroxidase (CCP) chromogenic enzyme was inserted into a wild type PP01 phage genome to construct the recombinant PP01ccp phage that was used in the production of the chromogenic enzyme through specific infection into E. coli O157:H7. The method was then examined in the colorimetric detection of E. coli O157:H7 in broth, and the appearance of E. coli O157:H7 in broth was confirmed by the color change after a few minutes of the enzyme assay. Secondly, the method was investigated in the colorimetric detection of E. coli O157:H7 in apple juice. A low E. coli O157:H7 concentration as 1 CFU mL(-1) was detected in 15 h that was in a shorter time than in previous bioluminescence phage-based methods. Moreover, the method is much simpler compared to other previous phage-based methods since it enables detection without the need for expensive apparatus.
authors.affiliation: Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh City University of Technology.
authors.forename: Hoang A
authors.initials: HA
authors.lastname: Hoang
authors.forename: Le T
authors.initials: le T
authors.lastname: Dien
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development; D002253:Carbonated Beverages / Q000382:microbiology*; D003124:Colorimetry / Q000379:methods*; D003578:Cytochrome-c Peroxidase / Q000032:analysis / Q000235:genetics; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000821:virology*; D005516:Food Microbiology / Q000379:methods*; D017930:Genes, Reporter; D016679:Genome, Viral; D027845:Malus; D011995:Recombination, Genetic; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D003578:Cytochrome-c Peroxidase