title: In vitro evaluation of random and aligned polycaprolactone/gelatin fibers via electrospinning for bone tissue engineering.
abstract: Scaffold, as an essential element of tissue engineering, should provide proper chemical and structural cues to direct tissue regeneration. In this study, aligned and random polycaprolactone (PCL)/gelatin fibrous scaffolds with different mass ratio were electrospun. Chemical, structural, and mechanical properties of PCL/gelatin fibrous scaffolds were characterized by FTIR and tensile measurements. The average diameters of different groups were between 334.96 ± 41.43 nm and 363.78 ± 50.49 nm. Blending PCL with gelatin increased the mechanical properties of the scaffolds. The cell culture results demonstrated that the mass ratio of PCL and gelatin showed no obvious effects on cell behavior, whereas the cell growth behavior was affected by the fibers orientation. Higher elongation ratio, enhanced cell proliferation and elevated alkaline phosphatase activity were observed for cells cultured on aligned fibers. The findings in our research provide insightful information for the design and fabrication of scaffolds for bone tissue engineering.
authors.affiliation: a Department of Materials Science and Engineering , Jinan University , 601 W Huangpu Ave, Guangzhou , P.R. China.
authors.forename: Zhenzhao
authors.initials: Z
authors.lastname: Guo
authors.forename: Jiaming
authors.initials: J
authors.lastname: Xu
authors.forename: Shan
authors.initials: S
authors.lastname: Ding
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Changren
authors.initials: C
authors.lastname: Zhou
authors.forename: Lihua
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005780:Gelatin / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D011091:Polyesters / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin
keywords: aligned fiber; electrospinning; gelatin; osteoblast; polycaprolactone