title: The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks' gestation.
abstract: CD200 expression in murine trophoblast and decidua prevents semi-allogeneic and LPS-induced abortions by binding to CD200 receptor-bearing cells to suppress NK activity, induces IDO in macrophages, and promotes the generation of regulatory T cell subsets. CD200 and its receptor CD200R1 reported in 7-9 weeks' gestation human villus trophoblasts are reduced in spontaneous abortion syncytiotrophoblasts. By specific antibody staining, we find that both CD200 and CD200R1 are expressed even earlier, by 5 weeks' gestation, by villus trophoblasts and by decidual cells. Expression of CD200 was validated using two independent antibodies. CD200-CD200R1 signaling may be required for human pregnancy success.
authors.affiliation: Department of Medicine, McMaster University, Health Sciences Center Rm. 3H1E, 1280 Main St. West, Hamilton, Ontario, Canada L8S 4K1; Toronto General Research Institute, Toronto, Ontario, Canada; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada. Electronic address: clarkd@mcmaster.ca.
authors.forename: David A
authors.initials: DA
authors.lastname: Clark
authors.affiliation: Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Arredondo
authors.affiliation: Department of Oncology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Sukhbinder
authors.initials: S
authors.lastname: Dhesy-Thind
mesh_terms: D015703:Antigens, CD / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002824:Chorionic Villi / Q000276:immunology*; D003656:Decidua / Q000276:immunology*; D005260:Female; D005333:Fetus / Q000276:immunology*; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D005865:Gestational Age; D006801:Humans; D064446:Orexin Receptors; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; C042739:antigens, CD200
keywords: CD200; CD200 receptor; Decidua; Human pregnancy immunology; Villus trophoblasts