title: Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15.
abstract: Bacillus amyloliquefaciens L-H15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress Fusarium oxysporum and other soil-borne fungal plant pathogens. Two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. The two compounds were identified by tandem Q-TOF mass spectroscopy as C15 Iturin A (1) and a novel cyclic peptide with a molecular weight of 852.4 Da (2). Both compounds showed good inhibitory activities against three plant fungal pathogens in cylinder-plate diffusion assay. To our best knowledge, this is the first report on a cyclic antifungal peptide with a molecular weight of 852.4 Da. The strong antifungal activity suggests that the B. amyloliquefaciens L-H15 and its bioactive components might provide an alternative resource for the biocontrol of plant diseases and sustainable agriculture.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Room 410, East District, 17# Qinghua East Road, Beijing, 100083, China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Han
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.forename: Chengzhen
authors.initials: C
authors.lastname: You
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Yu
authors.forename: Pinglan
authors.initials: P
authors.lastname: Li
authors.forename: Qingmao
authors.initials: Q
authors.lastname: Shang
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D002855:Chromatography, Thin Layer; D005658:Fungi / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
chemical_list: D000935:Antifungal Agents; D010456:Peptides, Cyclic