title: Complement regulatory protein genes in channel catfish and their involvement in disease defense response.
abstract: Complement system is one of the most important defense systems of innate immunity, which plays a crucial role in disease defense responses in channel catfish. However, inappropriate and excessive complement activation could lead to potential damage to the host cells. Therefore the complement system is controlled by a set of complement regulatory proteins to allow normal defensive functions, but prevent hazardous complement activation to host tissues. In this study, we identified nine complement regulatory protein genes from the channel catfish genome. Phylogenetic and syntenic analyses were conducted to determine their orthology relationships, supporting their correct annotation and potential functional inferences. The expression profiles of the complement regulatory protein genes were determined in channel catfish healthy tissues and after infection with the two main bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. The vast majority of complement regulatory protein genes were significantly regulated after bacterial infections, but interestingly were generally up-regulated after E. ictaluri infection while mostly down-regulated after F. columnare infection, suggesting a pathogen-specific pattern of regulation. Collectively, these findings suggested that complement regulatory protein genes may play complex roles in the host immune responses to bacterial pathogens in channel catfish.
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Jiang
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Jiaren
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Shikai
authors.initials: S
authors.lastname: Liu
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA; College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Xiaozhu
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA. Electronic address: liuzhan@auburn.edu.
authors.forename: Zhanjiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002397:Catfishes / Q000235:genetics* / Q000276:immunology*; D003167:Complement Activation / Q000235:genetics*; D003165:Complement System Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D020610:Edwardsiella ictaluri / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D005417:Flavobacterium / Q000276:immunology; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D017434:Protein Structure, Tertiary / Q000235:genetics
chemical_list: D029941:Fish Proteins; D006023:Glycoproteins; D003165:Complement System Proteins
keywords: Catfish; Complement regulatory protein; Disease; Fish; Genome; Innate immunity