title: Herd immunity and fatal cases of influenza among the population exposed to poultry and wild birds in Russian Asia in 2013-2014.
abstract: In total 1,525 blood serum samples were collected in October, 2013 in Russian Asia from people who reside in territories that are at high risk for emergence of influenza viruses with pandemic potential. Presence of antibodies to influenza viruses in the sera was tested in hemagglutination inhibition test. None of the samples produced positive results with the antigens A/H5 and A/H7. Twelve strains of influenza A(H1N1pdm09) virus were isolated from people who died presumably from influenza during 2013-2014 epidemic season. All strains were similar to vaccine strain A/California/07/09 according to their antigenic properties and sensitivity to anti-neuraminidase drugs (oseltamivir and zanamivir). Genetic analysis revealed that all strains belong to group 6, subgroup 6B of influenza A(H1N1)pdm09 virus. Substitutions in HA1: S164F add E235K as well as E47G, A86V, K331R, N386K, N397K in NA, and K131E, N29S in NS1, and N29S, R34Q in NEP separate investigated strains into two groups: 1st group-A/Chita/1114/2014, A/Chita/1115/2014, A/Chita/853/2014, A/Barnaul/269/2014 and 2nd group-A/Chita/655/2014, A/Chita/656/2014, A/Chita/709/2014, A/Chita/873/2014. Mutation D222G in HA1, which is often associated with high morbidity of the illness, was present in strain A/Novosibirsk/114/2014. Substitution N386K in NA removes a potential N-glycosylation site in neuraminidases of A/Chita/1114/2014, A/Chita/1115/2014, A/Chita/853/2014, A/Barnaul/269/2014, A/Novosibirsk/114/2014, and A/Blagoveshensk/252/2014.
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Ilyicheva
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Murat
authors.initials: M
authors.lastname: Abdurashitov
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Durymanov
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Susloparov
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Goncharova
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Kolosova
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Valery
authors.initials: V
authors.lastname: Mikheev
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Ryzhikov
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001717:Birds; D002648:Child; D002675:Child, Preschool; D004781:Environmental Exposure; D005260:Female; D005838:Genotype; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D032521:Immunity, Herd; D007223:Infant; D007231:Infant, Newborn; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000276:immunology* / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D011200:Poultry; D012800:Siberia / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus
keywords: human immunity; influenza viruses; phylogenic analysis; sequencing