title: Effects of ischemia and omeprazole preconditioning on functional recovery of isolated rat heart.
abstract: OBJECTIVE
  The aim of this study was to compare protective effects of ischemic and potential protective effects of pharmacological preconditioning with omeprazole on isolated rat heart subjected to ischemia/reperfusion.
  
  
  METHODS
  The hearts of male Wistar albino rats were excised and perfused on a Langendorff apparatus. In control group (CG) after stabilization period, hearts were subjected to global ischemia (perfusion was totally stopped) for 20 minutes and 30 minutes of reperfusion. Hearts of group II (IPC) were submitted to ischemic preconditioning lasting 5 minutes before 20 minutes of ischemia and 30 minutes of reperfusion. In third group (OPC) hearts first underwent preconditioning lasting 5 minutes with 100 μM omeprazole, and then submitted 20 minutes of ischemia and 30 minutes of reperfusion.
  
  
  RESULTS
  Administration of omeprazole before ischemia induction had protective effect on myocardium function recovery especially regarding to values of systolic left ventricular pressure and dp/dt max. Also our findings are that values of coronary flow did not change between OPC and IPC groups in last point of reperfusion.
  
  
  CONCLUSIONS
  Based on our results it seems that ischemic preconditioning could be used as first window of protection after ischemic injury especially because all investigated parameters showed continuous trend of recovery of myocardial function. On the other hand, preconditioning with omeprazole induced sudden trend of recovery with positive myocardium protection, although less effective than results obtained with ischemic preconditioning not withstand, we must consider that omeprazole may be used in many clinical circumstances where direct coronary clamping for ischemic preconditioning is not possible.
authors.affiliation: Department of Pharmaceutical chemistry, Faculty of Medical Sciences, University of Kragujevac, Serbia.
authors.forename: Nevena
authors.initials: N
authors.lastname: Jeremic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia.
authors.forename: Anica
authors.initials: A
authors.lastname: Petkovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Srejovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zivkovic
authors.affiliation: Institute of Medical Physiology "Richard Burian", School of Medicine, University of Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Djuric
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jakovljevic
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D003326:Coronary Circulation / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009853:Omeprazole / Q000494:pharmacology* / Q000627:therapeutic use; D054328:Proton Pump Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D054328:Proton Pump Inhibitors; D009853:Omeprazole