title: Improvement in glucose biosensing response of electrochemically grown polypyrrole nanotubes by incorporating crosslinked glucose oxidase.
abstract: In this paper a novel enzymatic glucose biosensor has been reported in which platinum coated alumina membranes (Anodisc™s) have been employed as templates for the growth of polypyrrole (PPy) nanotube arrays using electrochemical polymerization. The PPy nanotube arrays were grown on Anodisc™s of pore diameter 100 nm using potentiostatic electropolymerization. In order to optimize the polymerization time, immobilization of glucose oxidase (GOx) was first performed using physical adsorption followed by measuring its biosensing response which was examined amperometrically for increasing concentrations of glucose. In order to further improve the sensing performance of the biosensor fabricated for optimum polymerization duration, enzyme immobilization was carried out using cross-linking with glutaraldehyde and bovine serum albumin (BSA). Approximately six fold enhancement in the sensitivity was observed in the fabricated electrodes. The biosensors also showed a wide range of linear operation (0.2-13 mM), limit of detection of 50 μM glucose concentration, excellent selectivity for glucose, notable reliability for real sample detection and substantially improved shelf life.
authors.affiliation: Molecular and Nanoelectronics Research Group (MNRG), Department of Electrical Engineering, Indian Institute of Technology Indore, Pithampur Auto Cluster Limited (PACL) Building, Survey No. 113/2-B, Village Hernia Khedi, Mhow, Indore, Madhya Pradesh, India. Electronic address: agarpragya@gmail.com.
authors.forename: Pragya Agar
authors.initials: PA
authors.lastname: Palod
authors.affiliation: Molecular and Nanoelectronics Research Group (MNRG), Department of Electrical Engineering, Indian Institute of Technology Indore, Pithampur Auto Cluster Limited (PACL) Building, Survey No. 113/2-B, Village Hernia Khedi, Mhow, Indore, Madhya Pradesh, India.
authors.forename: Vipul
authors.initials: V
authors.lastname: Singh
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D005949:Glucose Oxidase; D005947:Glucose
keywords: Cross-linking; Glucose biosensor; Glucose oxidase; Polypyrrole; Porosity; Sensitivity