title: Complexation of cationic-neutral block polyelectrolyte with insulin and in vitro release studies.
abstract: Insulin (INS) was incorporated into complexes with the block polyelectrolyte quaternized poly[3,5-bis(dimethylaminomethylene)hydroxystyrene]-b-poly(ethylene oxide) (QNPHOSEO), which is a cationic-neutral block polyelectrolyte. Light scattering techniques are used in order to examine the size, the size distribution and the ζ-potential of the nanocarriers in aqueous and biological media, which are found to depend on the ratio of the components and the physicochemical parameters during and after complex preparation. Circular dichroism and infrared spectroscopy, employed to investigate the structure of the complexed INS, show no alteration of protein structure after complexation. In vitro release profiles of the entrapped protein are found to depend on the ratio of the components and the solution conditions used during preparation of the complexes.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Panepistimioupolis Zografou 15771, National and Kapodistrian University of Athens, Athens, Greece; Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue 11635, Athens, Greece.
authors.forename: Natassa
authors.initials: N
authors.lastname: Pippa
authors.affiliation: Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue 11635, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Karayianni
authors.affiliation: Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue 11635, Athens, Greece. Electronic address: pispas@eie.gr.
authors.forename: Stergios
authors.initials: S
authors.lastname: Pispas
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Panepistimioupolis Zografou 15771, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Costas
authors.initials: C
authors.lastname: Demetzos
mesh_terms: D002412:Cations / Q000737:chemistry*; D002942:Circular Dichroism / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D000067493:Dynamic Light Scattering / Q000379:methods; D007328:Insulin / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002412:Cations; D004337:Drug Carriers; D007328:Insulin; D011108:Polymers; D012996:Solutions; D014867:Water; D011092:Polyethylene Glycols
keywords: Block polyelectrolyte; Complexation process; In vitro release studies; Insulin; Stealthiness