title: Extraction and Purification of R-phycoerythrin from Marine Red Algae.
abstract: This chapter focuses on the recovery of an R-Phycoerythrin (R-PE)-enriched fraction from marine algae. Since R-PE is a proteinaceous pigment, we have developed a simple and rapid two-step method devoted to the extraction and purification of R-PE from marine red algae. Here we describe a phosphate buffer extraction followed by anion exchange chromatography carried on a DEAE Sepharose Fast Flow column. To ensure the quality and quantity of R-PE recovery, we also indicate different methods to monitor each fraction obtained, such as spectrophotometric indicators, gel filtration, and SDS-PAGE analysis.
authors.affiliation: LUNAM Université, Université de Nantes, MMS, 2 rue de la Houssinière, BP 92208, Nantes, 44322, France, justine.dumay@univ-nantes.fr.
authors.forename: Justine
authors.initials: J
authors.lastname: Dumay
authors.forename: Michèle
authors.initials: M
authors.lastname: Morançais
authors.forename: Huu Phuo Trang
authors.initials: HP
authors.lastname: Nguyen
authors.forename: Joël
authors.initials: J
authors.lastname: Fleurence
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010799:Phycoerythrin / Q000737:chemistry / Q000302:isolation & purification*; D000461:Rhodophyta / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D010799:Phycoerythrin