title: VERO stable cell lines expressing full-length human epidermal growth factor receptors 2 and 3: platforms for subtractive phage display.
abstract: Cross-talk between human epidermal growth factor receptor 2 and 3 (HER2 and HER3) may potentially contribute to therapeutic resistance in human breast cancer. Subtractive phage display allows highly specific selection for antibody fragments directed against cells surface HER2 and HER3. The strategies to select conformation- and activation-specific antibodies against HER2 and HER3 require tightly regulated HER2 and HER3 expressing cells that allow controlled activation/inactivation of these receptors during panning procedures. To achieve this, first, we found that the VERO cell line is an appropriate cell line for heterogeneous expression of HER2 and HER3, and then we established a panel of VERO stable cell lines expressing high levels of HER2 and HER3 alone and in combination. We also showed that HER2 and HER3 expressed in VERO cells were biologically active and could form heterodimer following neuregulin1 treatment. The cell line established here not only provided platforms for phage display-based methods but also could be used in any HER-related studies.
authors.affiliation: 1 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Akbar
authors.initials: A
authors.lastname: Hedayatizadeh-Omran
authors.affiliation: 1 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .|2 Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Reza
authors.initials: R
authors.lastname: Valadan
authors.affiliation: 1 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .|2 Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Alireza
authors.initials: A
authors.lastname: Rafiei
authors.affiliation: 1 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .|2 Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Mohsen
authors.initials: M
authors.lastname: Tehrani
authors.affiliation: 1 Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh-Navaei
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D006801:Humans; D019151:Peptide Library; D055503:Protein Multimerization; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D020893:Receptor, ErbB-3 / Q000096:biosynthesis* / Q000235:genetics; D014709:Vero Cells
chemical_list: D019151:Peptide Library; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; C508053:ERBB2 protein, human; C581292:ERBB3 protein, human