title: The Ultrastructure of MCF-7 Breast Cancer Cells after Vasodilator-Stimulated Phosphoprotein Knockdown.
abstract: Inhibition of vasodilator-stimulated phosphoprotein (VASP) expression could modulate the adhesion and proliferation of breast cancer cells. However, the underlying mechanisms are not well defined. Here, we show that knockdown of the VASP changes the ultrastructure of human MCF-7 breast cancer cells. Transfection of VASP shRNA significantly lowered the expression of VASP protein in MCF-7 cells. In the shRNA-VASP group, immunofluorescence showed diminished presence of F-actin, and it was lower in the nucleus than in the cytoplasm. After VASP was inhibited, the MCF-7 cells were oval in shape with blunt lamellipodium, disappearance of the cristae of mitochondria, decreased microvilli and more vacuoles. Collectively, our findings elucidated the morphological mechanism that knockdown of the VASP changed the ultrastructure of MCF-7 cells.
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan University School of Basic Medical Sciences , Wuhan , Hubei , China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: b Division of Nephrology , Renmin Hospital of Wuhan University , Wuhan , Hubei , China , and.
authors.forename: Ke
authors.initials: K
authors.lastname: Su
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan University School of Basic Medical Sciences , Wuhan , Hubei , China .
authors.forename: Peng-Chao
authors.initials: PC
authors.lastname: Hu
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan University School of Basic Medical Sciences , Wuhan , Hubei , China .
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Gao
authors.affiliation: c Department of Oncology , Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan University , Wuhan , Hubei , China.
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Zhang
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan University School of Basic Medical Sciences , Wuhan , Hubei , China .
authors.forename: Lei
authors.initials: L
authors.lastname: Wei
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D008840:Microfilament Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010750:Phosphoproteins / Q000378:metabolism*; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D000098662:Vasodilator-Stimulated Phosphoprotein
keywords: Knockdown; MCF-7; VASP; ultrastructure changes