title: The effect of human leukocyte antigen G alleles on human papillomavirus infection and persistence in a cohort of HIV-positive pregnant women from Brazil.
abstract: Patients with compromised immune systems have more severe intraepithelial lesions and more rapid disease progression, in addition to increased risk for cervical cancer. Persistent infection by the human papillomavirus (HPV) is a necessary step in that process. By inducing expression of inhibitory ligands of natural killer cells, like HLA-G, HPV avoids the elimination of infected cells. Recent studies have investigated polymorphisms in HLA-G that may be associated with susceptibility to HPV infection and persistence. One hundred-forty HIV(+) pregnant women from Brazil had a DNA fragment comprising HLA-G exons 2-4 PCR-amplified, cloned, sequenced and analyzed for allele determination. Altogether, 22 alleles comprising 52 different genotypes were found. Four novel HLA-G alleles were characterized. We have not observed association of specific HLA-G alleles with HPV infection, but found a protective effect of the G:01:01:02 allele against the occurrence of intraepithelial lesions. In addition to describing new HLA-G alleles and defining new reference sequences, our data provide a better understanding of the impact of HLA-G alleles on HPV-related disease.
authors.affiliation: Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Brunna M
authors.initials: BM
authors.lastname: Alves
authors.affiliation: Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Prellwitz
authors.affiliation: Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Juliana D
authors.initials: JD
authors.lastname: Siqueira
authors.affiliation: Instituto de Ginecologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Ângela R
authors.initials: ÂR
authors.lastname: Meyrelles
authors.affiliation: Programa de Epidemiologia Clínica, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Anke
authors.initials: A
authors.lastname: Bergmann
authors.affiliation: Programa de Genética, Instituto Nacional de Câncer, Rio de Janeiro, Brazil; Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Héctor N
authors.initials: HN
authors.lastname: Seuánez
authors.affiliation: Instituto de Puericultura e Pediatria Martagão Gesteira, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Machado
authors.affiliation: Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil; Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Soares
authors.affiliation: Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil. Electronic address: esoares@inca.gov.br.
authors.forename: Esmeralda A
authors.initials: EA
authors.lastname: Soares
mesh_terms: D001938:Brazil; D015331:Cohort Studies; D060085:Coinfection / Q000235:genetics / Q000821:virology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D015658:HIV Infections / Q000235:genetics* / Q000821:virology; D059951:HLA-G Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D011230:Precancerous Conditions / Q000235:genetics* / Q000821:virology; D011247:Pregnancy; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
chemical_list: D059951:HLA-G Antigens
keywords: Genetic association; HIV; HLA-G; HPV; Novel alleles