title: Regulation of the plasma amino acid profile by leucine via the system L amino acid transporter.
abstract: Plasma concentrations of amino acids reflect the intracellular amino acid pool in mammals. However, the regulatory mechanism requires clarification. In this study, we examined the effect of leucine administration on plasma amino acid profiles in mice with and without the treatment of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) or rapamycin as an inhibitor of system L or mammalian target of rapamycin complex 1, respectively. The elevation of plasma leucine concentration after leucine administration was associated with a significant decrease in the plasma concentrations of isoleucine, valine, methionine, phenylalanine, and tyrosine; BCH treatment almost completely blocked the leucine-induced decrease in plasma amino acid concentrations. Rapamycin treatment had much less effects on the actions of leucine than BCH treatment. These results suggest that leucine regulates the plasma concentrations of branched-chain amino acids, methionine, phenylalanine, and tyrosine, and that system L amino acid transporters are involved in the leucine action.
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Zhen
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kitaura
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kadota
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kondo
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Minjun
authors.initials: M
authors.lastname: Xu
authors.affiliation: a Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Shimomura
mesh_terms: D027062:Amino Acid Transport System L / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000596:Amino Acids / Q000097:blood*; D000598:Amino Acids, Cyclic / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007930:Leucine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D000076222:Mechanistic Target of Rapamycin Complex 1; D051379:Mice; D046912:Multiprotein Complexes / Q000037:antagonists & inhibitors / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D013997:Time Factors
chemical_list: D027062:Amino Acid Transport System L; D000596:Amino Acids; D000598:Amino Acids, Cyclic; D046912:Multiprotein Complexes; C006504:2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases; D007930:Leucine; D020123:Sirolimus
keywords: BCH; branched-chain amino acids; plasma amino acid profile; rapamycin