title: Cytokine-induced killer cells combined with dendritic cells inhibited liver cancer cells.
abstract: OBJECTIVE
  To investigate the prognosis of advanced liver cancer patients treated with CIK-DCs and the mechanism of apoptosis of HEPG 2 cells.
  
  
  METHODS
  67 patients were enrolled in the study. Peripheral blood mononuclear cells (PBMCs) were separated, of which adherent PBMCs used granulocyte 2 macrophage colony2 stimulating factor (GM2CSF), tumor necrosis factor 2α (TNF2α), and interleukin 24 (IL24) to induce DCs, which were sensitized with antigen of autologous or exogenous cancer cells to obtain Ag-DCs; suspended PBMCs used interferon 2γ (IFN2γ), IL-2, and CD 3 monoclonal antibody (CD3mAb) respectively, to induce CIK cells. DCs and CIK cells were cultured together. Flow cytometry was used to detect the phenotypes of DCs and CIK cells, and the blood retransfused into patients. Western blot and flow cytometer were used to analyze the growth cycle of HepG 2 cells and the expression of BAX and PCNA.
  
  
  RESULTS
  No patients underwent complete remission, 5 obtained partial remission and 29 had stable disease. Of the 31 patients whose lesions could not be evaluated, 17 received effective treatment, showing that the immune response was enhanced. In vitro laboratory experiments revealed that DC-CIK cells markedly affected the growth cycle of HepG 2 cells. Analysis showed that DC-CIK cells enhanced the gene expression of BAX and inhibited the activity of PCNA.
  
  
  CONCLUSIONS
  Co-cultured DCs and CIK cells inhibit the proliferation and migration of liver cancer cells by down-regulating PCNA and up-regulating BAX. This approach may be an effective method to treat advanced liver cancer.
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Li
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: De-Hong
authors.initials: DH
authors.lastname: Huang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Center of Endoscopy Examination & Therapy, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Guo-He
authors.initials: GH
authors.lastname: Yan
authors.affiliation: Shanghai Claison Biotechnologic Limited Company Shanghai 201201, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Xian-Jun
authors.initials: XJ
authors.lastname: Tang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Xiao
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Shanghai Claison Biotechnologic Limited Company Shanghai 201201, China.
authors.forename: Xian-Yu
authors.initials: XY
authors.lastname: Wu
authors.affiliation: Department of Biotherapy and Hemo-Oncology, Chongqing Cancer Institute 181 Hanyu Road, Chongqing 400030, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiang
keywords: Bel associated protein (Bax); CDs-CIK; HEPG2 cells; liver cancer patients; proliferating cell nuclear antigen (PCNA)