title: Development of a method using high-performance liquid chromatographic fingerprint and multi-ingredients quantitative analysis for the quality control of Yangxinshi Pian.
abstract: A simple and reliable method of high-performance liquid chromatography with diode array detection method was developed for fingerprint analysis and simultaneous determination of six compounds including puerarin, salvianolic acid B, berberine hydrochloride, palmatine chloride, dehydrocorydaline, and icariin in the Chinese medicine preparation Yangxinshi Pian. The separation was performed on an Agilent Eclipse XDB-C18 reserved-phase column (250 mm × 4.6mm I.D., 5 μm) using gradient elution with 50 mmol/L monopotassium phosphate aqueous solution and methanol as mobile phase at a flow rate of 1.0 mL/min. The column operating temperature was set at 30°C, and the detection wavelength was 280 nm. The method was validated by linearity, precision, accuracy, stability, and recovery. For fingerprint analysis, 25 peaks were selected as the common peaks, and four kinds of similarities including cosine similarity (S), ratio of similarity (S'), projection content similarity (C), and content similarity (P) were applied to evaluate the quality consistency of different batches of Yangxinshi Pian. The results showed that the developed method was an efficient tool for quality evaluation of Yangxinshi Pian.
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Yerui
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Qiyuan
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Qingdao Growful Pharmaceutical Co., Ltd, Qingdao, China.
authors.forename: Zuolin
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Lianjun
authors.initials: L
authors.lastname: Luan
authors.affiliation: College of Pharmaceutical Sciences, ZhejiangUniversity, Hangzhou, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D057230:Limit of Detection; D000432:Methanol / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D011786:Quality Control; D012044:Regression Analysis; D015203:Reproducibility of Results; D014867:Water
chemical_list: D004365:Drugs, Chinese Herbal; D010710:Phosphates; D017680:Potassium Compounds; C000608333:yangxinshi pian; D014867:Water; C013216:potassium phosphate; D000432:Methanol
keywords: Fingerprint analysis; Multi-ingredient quantitative analysis; Quality control; Yangxinshi Pian