title: Reduced Pumilio-2 expression in patients with temporal lobe epilepsy and in the lithium-pilocarpine induced epilepsy rat model.
abstract: OBJECTIVE
  Drosophila Pumilio (Pum), a homolog of mammalian Pum2, plays an important role in translational regulation in the central nervous system (CNS), particularly for dendrite outgrowth and neuronal excitability. We investigated the expression pattern and cellular distribution of Pum2 in patients with drug-refractory temporal lobe epilepsy (TLE) and rats with lithium chloride-pilocarpine-induced epilepsy.
  
  
  METHODS
  Real-time quantitative PCR (RT-qPCR), Western blot, immunohistochemistry, and double-labeled immunofluorescence were utilized to determine the expression level and distribution of Pum2 in temporal neocortex tissues from patients with intractable TLE (n=20) and patients with severe head trauma (n=20) in addition to the hippocampus and adjacent cortex of rats with lithium chloride-pilocarpine-induced TLE and controls.
  
  
  RESULTS
  Pum2 was expressed in the cell bodies and dendrites of neurons but did not colocalize with glial fibrillary acidic protein-positive astrocytes or propidium iodide (PI) in nuclei. The expression of Pum2 was significantly reduced in patients and rats with TLE in comparison to controls (P<0.05).
  
  
  CONCLUSIONS
  Pum2 expression was less in patients with TLE and a rodent model of epilepsy, suggesting that decreased expression of Pum2 may be involved in the pathogenesis of TLE.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Xu-Ling
authors.initials: XL
authors.lastname: Wu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Yun-Yi
authors.initials: YY
authors.lastname: Huang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Jin-Xian
authors.initials: JX
authors.lastname: Yuan
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China. Electronic address: chenym1997@sina.com.
authors.forename: Yang-Mei
authors.initials: YM
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D003712:Dendrites / Q000378:metabolism; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D019579:Neocortex / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013702:Temporal Lobe / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D016601:RNA-Binding Proteins
keywords: Excitability; Lithium chloride–pilocarpine-induced epilepsy model; Plasticity; Pumilio-2; Temporal lobe epilepsy