title: Impact of caffeine on β cell proliferation and apoptosis under the influence of palmitic acid.
abstract: We examined the influence of caffeine on the proliferation and apoptosis of β cells cultured in vitro in the presence of the free fatty acid palmitic acid (PA). Different concentrations of caffeine (1-100 mM) and free fatty PA were added to cultured β cells. The MTT assay was used to analyze cell proliferative activity; flow cytometry was used to measure apoptosis and calculate the apoptosis rate. Compared with the blank control group, cells cultured with 500 mM PA for 24, 48, 72, and 96 h showed inhibition of pancreatic β cell proliferative activity. In the 10 and 25 mM caffeine groups cultured for 48, 72, and 96 h, β cell proliferative activity was much higher than that in the 500 mM PA group. The apoptosis rate in the 500 mM PA group was 40.55 ± 20.33%, which was higher than that in the blank control group. The apoptosis rates in the 10 and 25 mM caffeine group and the PA group were 19.12 ± 10.56 and 20.97 ± 9.75%, respectively, which was lower than that in the 500 mM PA group. At some concentrations, caffeine can improve free fatty PA levels and guide pancreatic β cell proliferation inhibition and cell apoptosis.
authors.affiliation: Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China yumin_lzz@yeah.net.
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: T
authors.initials: T
authors.lastname: Shen
authors.affiliation: Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: B L
authors.initials: BL
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002110:Caffeine / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000187:drug effects*; D019308:Palmitic Acid / Q000378:metabolism*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007328:Insulin; D019308:Palmitic Acid; D002110:Caffeine