title: PEGylated ceria nanoparticles used for radioprotection on human liver cells under γ-ray irradiation.
abstract: Ceria nanoparticles (CNPs) have recently been shown to protect cells and animals from radiation-induced damage. However, most of the CNPs used in previous studies were either naked or weakly protected by surfactants, which inevitably encounter many obstacles in biological applications. Here, alendronate was used as an ideal anchor to graft polyethylene glycol (PEG) onto CNPs, leading to enhanced stability, reduced cytotoxicity, and improved biological properties. Further investigation assessed the protective ability of the nanoparticles against radiation-induced effects for human normal liver cells (L-02), indicating that the PEGylated CNPs (CNPs-AL-PEG) were more efficient than naked CNPs. We determined that enhanced Ce(3+)/Ce(4+) ratios improved intracellular dispersion and that the ameliorated intracellular distribution of CNPs-AL-PEG contributes to the elevated expression of SOD2, which leads to increased protection of normal cells against ROS and reduces the oxidatively generated DNA damage. These studies hold tremendous promise for radioprotection and biological applications.
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhang-You
authors.initials: ZY
authors.lastname: Yang
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Zeng
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Hao
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Radiation Oncology, Sichuan Cancer Hospital, Chengdu 610041, China. Electronic address: wwdwyl@sina.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Wang
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China. Electronic address: lrong361@126.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002563:Cerium / Q000008:administration & dosage / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D022781:Hepatocytes / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D002563:Cerium; D011092:Polyethylene Glycols
keywords: Ceria nanoparticles; PEGylation; Radioprotection; Reactive oxygen species; Superoxide dismutase