title: iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians.
abstract: The planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on clarifying the molecular mechanism of regeneration initiation in planarians at transcriptional level, but the initiation mechanism of planarian head regeneration (PHR) remains unclear at the protein level. Here, a global analysis of proteome dynamics during the early stage of PHR was performed using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics strategy, and our data are available via ProteomeXchange with identifier PXD002100. The results showed that 162 proteins were differentially expressed at 2 h and 6 h following amputation. Furthermore, the analysis of expression patterns and functional enrichment of the differentially expressed proteins showed that proteins involved in muscle contraction, oxidation reduction and protein synthesis were up-regulated in the initiation of PHR. Moreover, ingenuity pathway analysis showed that predominant signaling pathways such as ILK, calcium, EIF2 and mTOR signaling which were associated with cell migration, cell proliferation and protein synthesis were likely to be involved in the initiation of PHR. The results for the first time demonstrated that muscle contraction and ILK signaling might played important roles in the initiation of PHR at the global protein level. The findings of this research provide a molecular basis for further unraveling the mechanism of head regeneration initiation in planarians.
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Geng
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China; College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China; College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Qin
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China; College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Xiayan
authors.initials: X
authors.lastname: Zang
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China; College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China; College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Geng
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Xue
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Zimei
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Kexue
authors.initials: K
authors.lastname: Ma
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, Henan Province, China; Henan Engineering Laboratory for Bioengineering and Drug Development, Henan Normal University, Xinxiang, Henan Province, China; College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D006257:Head / Q000502:physiology*; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000235:genetics; D010455:Peptides / Q000032:analysis; D010932:Planarians / Q000235:genetics / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology*; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014945:Wound Healing / Q000502:physiology
chemical_list: D015801:Helminth Proteins; D010455:Peptides; D017346:Protein Serine-Threonine Kinases