title: Improved antifungal activity of amphotericin B-loaded TPGS-b-(PCL-ran-PGA) nanoparticles.
abstract: To develop amphotericin B-loaded biodegradable TPGS-b-(PCL-ran-PGA) nanoparticles (PLGA-TPGS-AMB NPs) for fungal infection treatment, PLGA-TPGS NPs and PLGA NPs were synthesized by a modified double emulsion method and characterized in terms of size and size distribution, morphology and zeta potential. Drug encapsulation efficiency, in vitro drug release, and in vitro/vivo tests against Candida glabrata were completed. The data showed that both of the two AMB-loaded NPs (PLGA-AMB NPs, PLGA-TPGS-AMB NPs) achieved significantly higher level of antifungal effects than water suspended AMB. In comparison with PLGA-AMB NPs, PLGA-TPGS-AMB NPs had a stronger protective effect against candidiasis and gained an advantage of prolonged antifungal efficacy. In conclusion, PLGA-TPGS-AMB NPs system significantly improves AMB bioavailability by increasing the aqueous dispersibility and improving the antifungal activity. And this would be an excellent choice for the antifungal treatment of the entrapped drug because of its low toxicity and higher effectiveness.
authors.affiliation: Stem Cell Engineering Research Center, School of Medical, Anhui University of Science & Technology Huainan 232001, Anhui, P.R. China ; State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University Wuhan 430071, P.R. China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Clinical Medical Laboratory, Shanghai Pudong New Area People's Hospital Huai'an, Shanghai 201299, P.R. China.
authors.forename: Ronghong
authors.initials: R
authors.lastname: Jiao
authors.affiliation: School of Bioteclmology, Southern Medical University Guangzhou 510515, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Xie
authors.affiliation: Stem Cell Engineering Research Center, School of Medical, Anhui University of Science & Technology Huainan 232001, Anhui, P.R. China.
authors.forename: Lifa
authors.initials: L
authors.lastname: Xu
authors.affiliation: Stem Cell Engineering Research Center, School of Medical, Anhui University of Science & Technology Huainan 232001, Anhui, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huo
authors.affiliation: Stem Cell Engineering Research Center, School of Medical, Anhui University of Science & Technology Huainan 232001, Anhui, P.R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Dai
authors.affiliation: Stem Cell Engineering Research Center, School of Medical, Anhui University of Science & Technology Huainan 232001, Anhui, P.R. China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Qian
authors.affiliation: School of Bioteclmology, Southern Medical University Guangzhou 510515, China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University Wuhan 430071, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: Stem Cell Engineering Research Center, School of Medical, Anhui University of Science & Technology Huainan 232001, Anhui, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Clinical Laboratory, The Affiliated Huai'an Hospital of Xuzhou Medical College Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
keywords: Antifungal; Candida glabrata; TPGS-b-(PCL-ran-PGA); amphotericin B; nanoparticle