title: Dietary fish oil inhibits mechanical allodynia and thermal hyperalgesia in diabetic rats by blocking nuclear factor-κB-mediated inflammatory pathways.
abstract: One of the most common complications of early-onset diabetes mellitus is peripheral diabetic neuropathy, which is manifested either by loss of nociception or by allodynia and hyperalgesia. Diabetes mellitus is a common metabolic disease in human beings with characteristic symptoms of hyperglycemia, chronic inflammation and insulin resistance. Dietary fatty acids, especially polyunsaturated fatty acids, have been shown anti-inflammatory role in various experimental conditions. The present study investigated the effects of fish oil supplementation on the inflammation in the dorsal root ganglion (DRG) of streptozotocin (STZ)-induced diabetes rats. The effects of diabetes and fish oil treatment on the allodynia and hyperalgesia were also evaluated. Dietary fish oil effectively attenuated both allodynia and hyperalgesia induce by STZ injection. Along with the behavioral findings, DRG from fish oil-treated diabetic rats displayed a decrease in inflammatory cytokines and the expression of nuclear factor-κB (NF-κB) compared with untreated diabetic rats. Fish oil supplementation also increased the phosphorylation of AKT in DRG of diabetic rats. These results suggested that dietary fish oil-inhibited allodynia and hyperalgesia in diabetic rats may stem from its anti-inflammatory potential by regulating NF-κB and AKT. Fish oil might be useful as an adjuvant therapy for the prevention and treatment of diabetic complications.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, the Fourth Military Medical University, Xi'an 710032, P.R. China.
authors.forename: Meng-Ying
authors.initials: MY
authors.lastname: Li
authors.affiliation: Department of Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, P.R. China.
authors.forename: Ya-Yun
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, the Fourth Military Medical University, Xi'an 710032, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, the Fourth Military Medical University, Xi'an 710032, P.R. China.
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Hou
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, the Fourth Military Medical University, Xi'an 710032, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, the Fourth Military Medical University, Xi'an 710032, P.R. China. Electronic address: fmmur.young@gmail.com.
authors.forename: Rui-Hua
authors.initials: RH
authors.lastname: Yang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, the Fourth Military Medical University, Xi'an 710032, P.R. China. Electronic address: wfeng@fmmu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000503:physiopathology; D019587:Dietary Supplements; D005395:Fish Oils / Q000737:chemistry / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D006930:Hyperalgesia / Q000178:diet therapy* / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000178:diet therapy / Q000378:metabolism; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D016207:Cytokines; D005395:Fish Oils; D020672:I-kappa B Proteins; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D013311:Streptozocin; D051057:Proto-Oncogene Proteins c-akt
keywords: Diabetes; Fish oil; Inflammation; NF-κB; Painful diabetic neuropathy