title: Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm.
abstract: BACKGROUND
  Yersinia pestis, causing deadly plague, is classified as a group A bioterrorism bacterium. Some recent DNA-based methods were used for detection of bioterrorism agents.
  
  
  RESULTS
  Y. pestis was used as a model organism to develop an immunosensor based on surface plasmon resonance imaging (SPRi) using monoclonal antibody against Y. pestis F1 antigen. The experimental approach included step-by-step detection of Y. pestis membrane proteins, lysed bacteria, intact bacteria, mock-infected powder and mock-infected clinical specimens. SPRi detected on average 10(6) intact Y. pestis organisms in buffer, in mock-infected powder and in a 1:4 mixture with HEL cells.
  
  
  CONCLUSIONS
  This study offers the proof-of-concept of the SPRi-based detection of a human pathogen in both environmental and clinical specimens.
authors.affiliation: Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France. tt.hong.huynh@gmail.com.
authors.forename: Hong T T
authors.initials: HT
authors.lastname: Huynh
authors.affiliation: Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France. guillaume.gotthard@gmail.com.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gotthard
authors.affiliation: Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France. jerome.terras@univ-amu.fr.
authors.forename: Jérome
authors.initials: J
authors.lastname: Terras
authors.affiliation: Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France. gerard.aboudharam@gmail.com.
authors.forename: Gérard
authors.initials: G
authors.lastname: Aboudharam
authors.affiliation: Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France. michel.drancourt@univ-amu.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Drancourt
authors.affiliation: Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France. eric.chabriere@univ-amu.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Chabrière
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D054045:Biological Warfare Agents; D006801:Humans; D010930:Plague / Q000175:diagnosis / Q000382:microbiology; D020349:Surface Plasmon Resonance; D015010:Yersinia pestis / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D054045:Biological Warfare Agents; C086725:caf1 protein, Yersinia pestis