title: Immunological Response to Single Pathogen Challenge with Agents of the Bovine Respiratory Disease Complex: An RNA-Sequence Analysis of the Bronchial Lymph Node Transcriptome.
abstract: Susceptibility to bovine respiratory disease (BRD) is multi-factorial and is influenced by stress in conjunction with infection by both bacterial and viral pathogens. While vaccination is broadly used in an effort to prevent BRD, it is far from being fully protective and cases diagnosed from a combination of observed clinical signs without any attempt at identifying the causal pathogens are usually treated with antibiotics. Dairy and beef cattle losses from BRD are profound worldwide and genetic studies have now been initiated to elucidate host loci which underlie susceptibility with the objective of enabling molecular breeding to reduce disease prevalence. In this study, we employed RNA sequencing to examine the bronchial lymph node transcriptomes of controls and beef cattle which had individually been experimentally challenged with bovine respiratory syncytial virus, infectious bovine rhinotracheitis, bovine viral diarrhea virus, Pasteurella multocida, Mannheimia haemolytica or Mycoplasma bovis to identify the genes that are involved in the bovine immune response to infection. We found that 142 differentially expressed genes were located in previously described quantitative trait locus regions associated with risk of BRD. Mutations affecting the expression or amino acid composition of these genes may affect disease susceptibility and could be incorporated into molecular breeding programs. Genes involved in innate immunity were generally found to be differentially expressed between the control and pathogen-challenged animals suggesting that variation in these genes may lead to a heritability of susceptibility that is pathogen independent. However, we also found pathogen-specific expression profiles which suggest that host genetic variation for BRD susceptibility is pathogen dependent.
authors.affiliation: Embrapa Southeast Livestock, São Carlos, São Paulo, Brazil; Division of Animal Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Polyana C
authors.initials: PC
authors.lastname: Tizioto
authors.affiliation: Division of Animal Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: JaeWoo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Seabury
authors.affiliation: Division of Animal Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Schnabel
authors.affiliation: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Laurel J
authors.initials: LJ
authors.lastname: Gershwin
authors.affiliation: Department of Animal Science, College of Agriculture, University of California Davis, Davis, California, United States of America.
authors.forename: Alison L
authors.initials: AL
authors.lastname: Van Eenennaam
authors.affiliation: Department of Animal Science, College of Agriculture, University of California Davis, Davis, California, United States of America.
authors.forename: Rachel
authors.initials: R
authors.lastname: Toaff-Rosenstein
authors.affiliation: Department of Animal Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, United States of America.
authors.forename: Holly L
authors.initials: HL
authors.lastname: Neibergs
authors.affiliation: Division of Animal Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Jeremy F
authors.initials: JF
authors.lastname: Taylor
mesh_terms: D000818:Animals; D048090:Bovine Respiratory Disease Complex / Q000276:immunology*; D001980:Bronchi / Q000473:pathology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000821:virology*; D016000:Cluster Analysis; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D008198:Lymph Nodes / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D025341:Principal Component Analysis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011786:Quality Control; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D051193:Toll-Like Receptors