title: The interactome of soybean GmWRKY53 using yeast 2-hybrid library screening to saturation.
abstract: Soybean GmWRKY53 functions in both biotic and abiotic stress signaling. Using GmWRKY53 as a bait yeast 2-hybrid library screening to saturation isolated multiple independent fragments for many interacting proteins, enabling delineation of minimal interacting domains and computation of a confidence score. Multiple independent clones coding for the LATE ELONGATED HYPOCOTYL clock protein GmLCL2 (MYB114) were isolated and the binding site for GmWRKY53 was mapped to 90 amino acids separate from the MYB domain. This suggests a direct input from the clock on GmWRKY53 activity. The GmWRKY53-interacting proteins also included 3 water stress-inducible AP2/ERF transcription factors. One of these (Glyma03g26310) is one of the most strongly water stress induced genes in soybean roots, suggesting that GmWRKY53/ERF complexes regulate water stress responses.
authors.affiliation: a Molecular and Computational Biology Section; University of Southern California ; Los Angeles , CA USA.
authors.forename: Prateek
authors.initials: P
authors.lastname: Tripathi
authors.forename: Roel C
authors.initials: RC
authors.lastname: Rabara
authors.forename: Mani Kant
authors.initials: MK
authors.lastname: Choudhary
authors.forename: Marissa A
authors.initials: MA
authors.lastname: Miller
authors.forename: Ying-Sheng
authors.initials: YS
authors.lastname: Huang
authors.forename: Qingxi J
authors.initials: QJ
authors.lastname: Shen
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Blachon
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Rushton
mesh_terms: D000595:Amino Acid Sequence; D003681:Dehydration; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D013025:Glycine max / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins
keywords: ERF transcription factor; GTPase accelerating proteins, GAP; LATE ELONGATED HYPOCOTYL, LHY; WRKY transcription factor; bright yellow 2, BY-2; clock protein; drought; guanine nucleotide exchange factor, GEF; protein-protein interaction; resistance proteins, R proteins; soybean; transcription factor, TF; yeast two-hybrid, Y2H