title: ANP/NPRA signaling preferentially mediates Th2 responses in favor of pathological processes during the course of acute allergic asthma.
abstract: Although atrial natriuretic peptide (ANP) has been well recognized for its role in the regulation of volume-pressure homeostasis in cardiovascular system, its impact on respiratory system, particularly on the pathogenesis of acute allergic asthma, is yet to be elucidated. In the present report, we induced mice with OVA for onset of acute allergic asthma along with the administration of recombinant ANP or A71915 (an antagonist for ANP/natriuretic peptide receptor A, NPRA). It was noted that treatment of mice with ANP significantly promoted inflammatory infiltration in the airway and the production of inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) and lung homogenates, and the number of inflammatory cells in the BALF was significantly higher as compared with that of PBS treated asthmatic mice. Moreover, blockade of ANP/NPRA signaling by A71915 almost completely attenuated the effect of ANP administration. Mechanistic studies revealed that ANP repressed the expression of Th1 transcription factor T-bet, but enhanced Th2 transcription GATA3 expression. Together, our data provided feasible evidence suggesting that ANP/NPRA signaling predominantly induces a Th2-type response in favor of pathological processes during the course of acute allergic asthma.
authors.affiliation: Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University Guilin 541001, China ; Institute of Respiratory Diseases, Guilin Medical University Guilin 541001, China ; Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous Region Guilin 541001, China.
authors.forename: Libing
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University Guilin 541001, China ; Institute of Respiratory Diseases, Guilin Medical University Guilin 541001, China ; Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous Region Guilin 541001, China.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University Guilin 541001, China ; Institute of Respiratory Diseases, Guilin Medical University Guilin 541001, China ; Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous Region Guilin 541001, China.
authors.forename: Biwen
authors.initials: B
authors.lastname: Mo
authors.affiliation: Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University Guilin 541001, China ; Institute of Respiratory Diseases, Guilin Medical University Guilin 541001, China ; Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous Region Guilin 541001, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University Guilin 541001, China ; Institute of Respiratory Diseases, Guilin Medical University Guilin 541001, China ; Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous Region Guilin 541001, China.
authors.forename: Yabing
authors.initials: Y
authors.lastname: Sun
authors.affiliation: The Center for Biomedical Research, Tongji Hospital, Huazhong University of Science and Technology Wuhan 430030, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, China.
authors.forename: Shaokun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Emergency Medicine and Rare Diseases, Central South University Changsha 410011, China ; Department of Emergency Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Institute of Emergency Medicine and Rare Diseases, Central South University Changsha 410011, China ; Department of Emergency Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, China ; The Center for Biomedical Research, Tongji Hospital, Huazhong University of Science and Technology Wuhan 430030, China.
authors.forename: Cong-Yi
authors.initials: CY
authors.lastname: Wang
keywords: Atrial natriuretic peptide/Natriuretic peptide receptor A (ANP/NPRA) signaling; Th1/Th2 cells; acute allergic asthma; airway inflammation