title: Probing fibronectin-antibody interactions using AFM force spectroscopy and lateral force microscopy.
abstract: The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin-carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces strongly dominate over the non-specific ones. However, obtaining quantitative results requires the complex and tedious calibration of a torsional force. Here, a new and relatively simple method for the calibration of the torsional force is presented. The proposed calibration method is validated through the measurement of the interaction forces between human fibronectin and its monoclonal antibody. The results obtained using LFM and AFM-based classical force spectroscopies showed similar unbinding forces recorded at similar loading rates. Our studies verify that the proposed lateral force calibration method can be applied to study single molecule interactions.
authors.affiliation: Laboratoire de la Physique de la Matière Vivante, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Andrzej J
authors.initials: AJ
authors.lastname: Kulik
authors.affiliation: Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Lekka
authors.affiliation: Laboratoire de la Physique de la Matière Vivante, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: Kyumin
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Histology, Jagiellonian University Medical College, Kopernika 7, 31-034 Kraków, Poland.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Pyka-Fościak
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziądzka 5/7, 87-100 Toruń, Poland.
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Nowak
keywords: fibronectin; lateral force microscopy; molecular recognition; torsional forces calibration