title: PP029. Genetic change of adipose tissue related to inflammation in preeclampsia: Findings under novel culture method.
abstract: BACKGROUND
  Recently, adipose tissue is suggested to contribute to the inflammatory action in preeclampsia(PE), from peripheral increase of cytokines. However, the mechanism of the action in adipose tissue was not clarified yet.
  
  
  OBJECTIVE
  In this study, we performed "Gel bottom-captured" adipose tissue culture with PE to show that the adipose tissue is the inflammatory focus in the pathophysiology of PE.
  
  
  METHODS
  Under informed consent of the patients, omentum from probe laparotomy for ovarian cancer was captured in Peptide Hydrogel®. After 24h starvation, tissues were cultured with medium containing 10% of severe PE and healthy pregnant maternal serum (n=5 each). M30 (Apoptosis) and M65 (all cell death) were measured with ELISA. After mRNA extraction from tissue, quantitative PCR array (Qiagen, Inc.) was performed on all samples.
  
  
  RESULTS
  No significant histological differences were found between each culture. However, M30/M65 ratio was increased in PE (p=0.053). In PCR array, under 2-fold decrease in OSM (p=0.019) was found, and over 2-fold increase in TLR (p<0.01) and other inflammatory genes were defined in PE.
  
  
  CONCLUSIONS
  Inflammatory action in PE via TLR pathway was defined by adipose tissue culture. Apoptosis were shown in PE condition, but total tissue injury include necrosis were suggested to be stronger in normal pregnancy. Increase of inflammatory gene suggested that adipose tissue is one of a main organ of systemic inflammation in PE.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Naruse
authors.forename: Taihei
authors.initials: T
authors.lastname: Tsunemi
authors.forename: Akira
authors.initials: A
authors.lastname: Onogi
authors.forename: Natsuki
authors.initials: N
authors.lastname: Koike
authors.forename: Juria
authors.initials: J
authors.lastname: Akasaka
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Oi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi