title: Silk fibroin/sodium alginate composite nano-fibrous scaffold prepared through thermally induced phase-separation (TIPS) method for biomedical applications.
abstract: To mimic the natural fibrous structure of the tissue extracellular matrix, a nano-fibrous silk fibroin (SF)/sodium alginate (SA) composite scaffold was fabricated by a thermally-induced phase-separation method. The effects of SF/SA ratio on the structure and the porosity of the composite scaffolds were examined. Scanning electron microscopy and porosity results showed that the 5SF/1SA and 3SF/1SA scaffolds possessed an excellent nano-fibrous structure and a porosity of more than 90%. Fourier transform infrared, X-ray diffraction, and differential scanning calorimetry results indicated the physical interaction between SF and SA molecules and their good compatibility in the 5SF/1SA and 3SF/1SA scaffolds, whereas they showed less compatibility in the 1SF/1SA scaffold. Cell culture results showed that MG-63 cells can attach and grow well on the surface of the SF/SA scaffolds. The nano-fibrous SF/SA scaffold can be potentially used in tissue engineering.
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: zhp9810_a@163.com.
authors.forename: Haiping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: xtianliu@126.com.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: yangm@zju.edu.cn.
authors.forename: Mingying
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: ljzhu@zju.edu.cn.
authors.forename: Liangjun
authors.initials: L
authors.lastname: Zhu
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx / Q000737:chemistry; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002460:Cell Line; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D057139:Nanofibers / Q000737:chemistry*; D016062:Porosity; D047011:Silk / Q000737:chemistry*; D017670:Sodium Compounds / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D047011:Silk; D017670:Sodium Compounds; C513667:sodium aluminum hydride; D005349:Fibroins
keywords: Cell biocompatibility; Nano-fibrous; Silk fibroin; Sodium alginate