title: [Effect of SAHA on Maturation of Dendritic Cells and Its Mechanism].
abstract: OBJECTIVE
  To investigate the effect of SAHA on the maturation of human dendritic cells (DC) and to explore its underlying mechanism.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMNC) were isolated from human peripheral blood and cultured in RPMI 1640 medium with 100 ng/ml rhGM-CSF and 500 U/ml rhIL-4. In the LPS induced maturation process, dendritic cells treated with or without SAHA were used as test group, and dendritic cells treated without LPS or SAHA were used as control group. DC was observed under inverted microscope. Flow cytometer was used to detect the surface antigen molecules expressed by DC. The mixed lymphocyte culture (MLC) was used to observe the allogeneic lymphocyte stimulation. The NF-κB signaling pathway was detected by electrophoretic mobility shift assay (EMSA).
  
  
  RESULTS
  The SAHA could effectively suppress the maturation of DC induced by LPS, the DC treated with SAHA+LPS had immature morphological characteristics; the expression of CD80, CD83 and HLA-DR in SAHA+LPS group and control group were significantly down-regulated as compared with single LPS group (P<0.01); the ability of DC to stimulate the proliferation of allogeneic T lymphocytes in SAHA+LPS group and control group was significantly weaker than that in single LPS group (P<0.01); EMSA results showed that NF-κB activity decreased after SAHA and LPS treatment and was significantly lower than that of single LPS group.
  
  
  CONCLUSIONS
  SAHA can effectively suppress DC maturation induced by LPS and also weaken the ability to stimulate allogeneic T lymphocyte. NF-κB signaling pathway is involved in regulating DC maturation.
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Fu
authors.affiliation: Department of Clinical Laboratory Examination, Huaihe Hospital, Henan University, Kaifeng 475000, Henan Province, China.
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Bai
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Jin-Zong
authors.initials: JZ
authors.lastname: Lin
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Lu
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Emergency, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China. E-mail: zhangkejie0378@sina.com.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation; D003713:Dendritic Cells; D005434:Flow Cytometry; D006684:HLA-DR Antigens; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D016328:NF-kappa B; D013601:T-Lymphocytes
chemical_list: D006684:HLA-DR Antigens; D016328:NF-kappa B