title: Distinct Metabolomic Profiles of Papillary Thyroid Carcinoma and Benign Thyroid Adenoma.
abstract: Papillary thyroid carcinoma (PTC) and benign thyroid adenoma (BTA) are the most common head and neck tumors. However, the metabolic differences between PTC and BTA have not been characterized. The aim of this study was to identify the metabolic profiles of these two types of tumors using a metabolomics approach. Tumors and adjacent nontumor specimens collected from 57 patients with PTC and 48 patients with BTA were profiled using gas chromatography-time-of-flight mass spectrometry and ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry. A panel of 46 and 44 differentially expressed metabolites were identified in the PTC and BTA specimens, respetively, and compared with nontumor tissues. Common metabolic signatures, as characterized by increased glycolysis, amino acid metabolism, one carbon metabolism and tryptophan metabolism, were found in both types of tumors. Purine and pyrimidine metabolism was significantly elevated in the PTC specimens, and taurine and hypotaurine levels were also higher in the PTC tissues. Increased fatty acid and bile acid levels were found, especially in the BTA tissues. The metabolic profiles of the PTC and BTA tissues include both similar and remarkably different metabolites, suggesting the presence of common and unique mechanistic pathways in these types of tumors during tumorigenesis.
authors.affiliation: †Department of Otolaryngology Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Yishan Road 600, 200233 Shanghai, China.|‡Department of Head and Neck Surgery, Renji Hospital of Shanghai Jiao Tong University School of Medicine, Shandongzhong Road 145,200001 Shanghai, China.|∥Otolaryngological Institute of Shanghai Jiao Tong University, Yishan Road 600, 200233 Shanghai, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: §Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Yishan Road 600, 200233 Shanghai, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: §Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Yishan Road 600, 200233 Shanghai, China.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: §Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Yishan Road 600, 200233 Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.affiliation: ‡Department of Head and Neck Surgery, Renji Hospital of Shanghai Jiao Tong University School of Medicine, Shandongzhong Road 145,200001 Shanghai, China.|∥Otolaryngological Institute of Shanghai Jiao Tong University, Yishan Road 600, 200233 Shanghai, China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Wang
authors.affiliation: †Department of Otolaryngology Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Yishan Road 600, 200233 Shanghai, China.|∥Otolaryngological Institute of Shanghai Jiao Tong University, Yishan Road 600, 200233 Shanghai, China.
authors.forename: Shankai
authors.initials: S
authors.lastname: Yin
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000596:Amino Acids / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000145:classification / Q000379:methods*; D008875:Middle Aged; D009367:Neoplasm Staging; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D000596:Amino Acids; D011687:Purines; D011743:Pyrimidines; D002244:Carbon; C030986:pyrimidine; C030985:purine
keywords: benign thyroid adenoma; gas chromatography−time-of-flight mass spectrometry; metabolomics; papillary thyroid carcinoma; ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry