title: Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau.
abstract: Rhodiola alsia, which has been used widely in traditional Chinese medicine for a considerable time, grows on moist habitats at high altitude near the snow line. Microsatellite loci were developed for R. alsia to investigate its population genetics. In total, 17 polymorphic microsatellites were developed based on ESTs from the Illumina HiSeq(TM) 2000 platform. The microsatellite loci were checked for variability using 80 individuals of R. alsia sampled from four locations on the Qinghai-Tibet Plateau. The total number of alleles per locus ranged from 10 to 20, and the observed heterozygosity ranged from 0.000 to 1.000. The null allele frequency ranged from 0.000 to 0.324. These microsatellites are expected to be helpful in future studies of population genetics in R. alsia and related species.
authors.affiliation: Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing, China.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lei
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: Q B
authors.initials: QB
authors.lastname: Gao
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: G
authors.initials: G
authors.lastname: Khan
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: R
authors.initials: R
authors.lastname: Xing
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China slchen@nwipb.cas.cn.
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000483:Alleles; D006801:Humans; D038861:Medicine, Tibetan Traditional; D018895:Microsatellite Repeats / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic; D029771:Rhodiola / Q000235:genetics*; D018609:Tibet