title: Probing the Mucoadhesive Interactions Between Porcine Gastric Mucin and Some Water-Soluble Polymers.
abstract: This study investigates the structural features of porcine gastric mucin (PGM) in aqueous dispersions and its interactions with water-soluble polymers (poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), poly(ethylene oxide), and poly(ethylene glycol)) using isothermal titration calorimetry, turbidimetric titration, dynamic light scattering, and transmission electron microscopy. It is established that PAA (450 kDa) and PMAA (100 kDa) exhibit strong specific interactions with PGM causing further aggregation of its particles, while PAA (2 kDa), poly(ethylene oxide) (1 000 kDa), and poly(ethylene glycol) (10 kDa) do not show any detectable effects on mucin. Sonication of mucin dispersions prior to their mixing with PAA (450 kDa) and PMAA (100 kDa) leads to more pronounced intensity of interactions.
authors.affiliation: Reading School of Pharmacy, University of Reading, Whiteknights PO Box 224, Reading, RG6 6AD, United Kingdom.
authors.forename: Yasser A
authors.initials: YA
authors.lastname: Albarkah
authors.affiliation: Reading School of Pharmacy, University of Reading, Whiteknights PO Box 224, Reading, RG6 6AD, United Kingdom.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Green
authors.affiliation: Reading School of Pharmacy, University of Reading, Whiteknights PO Box 224, Reading, RG6 6AD, United Kingdom. v.khutoryanskiy@reading.ac.uk.
authors.forename: Vitaliy V
authors.initials: VV
authors.lastname: Khutoryanskiy
mesh_terms: D000818:Animals; D005752:Gastric Mucins / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012995:Solubility; D013552:Swine; D014867:Water / Q000737:chemistry
chemical_list: D005752:Gastric Mucins; D011109:Polymethacrylic Acids; D014867:Water; C030613:polymethacrylic acid; D011092:Polyethylene Glycols; C000595209:polyethylene glycol 1000
keywords: hydrogen bonding; isothermal titration calorimetry; mucoadhesion; porcine gastric mucin; water-soluble polymers