title: Roscovitine treatment caused impairment of fertilizing ability in mice.
abstract: OBJECTIVE
  To explore the adverse effect of roscovitine on reproductive system of male mice.
  
  
  METHODS
  Male hSOD1(G93A) transgenetic mice received roscovitine 72 nmol/day (d) for 4 weeks (w), with normal control and dimethyl sulfoxide (DMSO)-treated animals served as controls (n=4). Male C57BL/6 mice were treated with roscovitine at either 72 nmol/d or 144 nmol/d for 4 w or 8 w, and normal control and DMSO treated mice served as controls. Fertility of male mice, sperm quality parameters, histological and related pathological changes of seminiferous tubules associated with roscovitine treatment were evaluated.
  
  
  RESULTS
  In male hSOD1(G93A) transgenetic mice treated with 72 nmol/d roscovitine for 4 w and C57BL/6 male mice treated with 72 nmol/d roscovitine for 8w and 144 nmol/d roscovitine for 4 w and 8 w, sperm counts and sperm motility rates decreased and sperm abnormality rates increased, and damage of seminiferous tubules were detected. Roscovitine treatment induced inhibition of CDK5 activities and decrease of BrdU-positive tubuler cells.
  
  
  CONCLUSIONS
  These results demonstrated that roscovitine treatment induced interference of male reproductive system and caused impairment of fertilizing ability. Reproductive system of ALS male mice was more susceptible to roscovitine induced impaired fertilizing ability.
authors.affiliation: Department of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Department of Neurology, The Affiliated Hospital of Weifang Medical University, Weifang 261000, Shandong, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ren
authors.affiliation: Department of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang, PR China.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Neurology, The First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang, PR China. Electronic address: fenghonglin1014@sina.com.
authors.forename: Honglin
authors.initials: H
authors.lastname: Feng
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: cuishangjin@126.com.
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D005298:Fertility / Q000187:drug effects*; D006728:Hormones / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011687:Purines / Q000009:adverse effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects*; D000077546:Roscovitine; D012671:Seminiferous Tubules / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013737:Testis / Q000187:drug effects / Q000378:metabolism
chemical_list: D006728:Hormones; D011687:Purines; D017382:Reactive Oxygen Species; D000077546:Roscovitine; D008315:Malondialdehyde; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1
keywords: Male mice; Reproductive toxicity; Roscovitine