title: Alterations in lipid profile, oxidative stress and hepatic function in rat fed with saccharin and methyl-salicylates.
abstract: BACKGROUND
  Food additives attract consumers, improve foods quality, control weight, and replace sugar in foods, while it may affect seriously children and adults health.
  
  
  OBJECTIVE
  To investigate the adverse effects of saccharin and methylsalicyltaes on lipid profile, blood glucose, renal, hepatic function, and oxidative stress/antioxidant (lipid peroxidation, Catalase and reduced glutathione (GSH) in liver tissues).
  
  
  METHODS
  46 young male albino rats were used. Saccharin and methylsalicylate were giving orally as low and high dose for 30 days. Rats were divided into 5 groups, 1(st) control group, 2(nd) and 3(rd) low and high saccharin-treated groups and 4(th) and 5(th) low and high methylsalicylate-treated group.
  
  
  RESULTS
  Serum total cholesterol, triglyceride, glucose levels and body weight gain were decreased in saccharin high dose compared to control. Rats ingested high dose of saccharin presented a significant reduction in serum triglycerides, cholesterol and LDL levels. Low and high doses of saccharin exhibited a significant increase in liver function marker of ALT, AST, ALP activity, total proteins and albumin levels and renal function test (urea and creatinine levels) in comparison with control group. Saccharin high dose induce a significant decline in hepatic GSH levels, catalase and SOD activities while increased in hepatic MDA level.
  
  
  CONCLUSIONS
  It could be concluded that, saccharin affects harmfully and alters biochemical markers in hepatic and renal tissues not only at greater doses but also at low doses. Whereas uses of metylsalicylates would not pose a risk for renal function and hepatic oxidative markers.
authors.affiliation: Department of Chemistry Biochemistry, Science College, University of Dammam KSA ; Department of Biochemistry, Faculty of Veterinary Medicine, Beni Suef University Egypt.
authors.forename: Kamal Adel
authors.initials: KA
authors.lastname: Amin
authors.affiliation: Department of Chemistry Biochemistry, Science College, University of Dammam KSA.
authors.forename: Hessah Mohammed
authors.initials: HM
authors.lastname: AlMuzafar
keywords: Food additives; liver; methyl-salicylates; oxidative biomarkers; saccharin