title: Utilization of coconut oil cake for the production of lipase using Bacillus coagulans VKL1.
abstract: The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In the first step, the influence of pH, temperature, carbon source, nitrogen source and inducers on lipase activity was investigated by the One-Factor-At-A-Time (OFAT) method. In the second step, the three significant factors resulted from OFAT were optimized by the statistical approach (CCD).The optimum values of olive oil (0.5%), Tween 80 (0.6%) and FeSO4 (0.05%) was found to be responsible for a 3.2-fold increase in the lipase production identified by Central Composite Design.
authors.affiliation: Department of Chemical Engineering, AC College of technology, Anna University.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Gowthami
authors.forename: Karuppan
authors.initials: K
authors.lastname: Muthukumar
authors.forename: Manickam
authors.initials: M
authors.lastname: Velan
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification; D002244:Carbon / Q000378:metabolism; D016000:Cluster Analysis; D000074263:Coconut Oil; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D010938:Plant Oils / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D008049:Lipase; D009584:Nitrogen; D000074263:Coconut Oil