title: The role of leptin in the ventricular remodeling process and its mechanism.
abstract: OBJECTIVE
  This study aims to explore the role of leptin in the ventricular remodeling process and its mechanism in the diabetic rats' model.
  
  
  METHODS
  The diabetic SD rats model induced by streptozotocin was established. The SD rats were randomly divided into 4 groups: control group (20 rats treated with citric acid/sodium citrate buffer); M0 group (10 rats treated with physiological saline); M1 group (10 rats treated with 50 μg/kg LP); M2 group (10 rats treated with 100 μg/kg LP). Ang-II was detected by ELISA. The expression levels of LP and Ob-Rb were detected by RT-PCR. MAPK phosphorylation changes were detected by western blotting. Myocardial morphology was observed.
  
  
  RESULTS
  Compared with control group, the blood glucose concentration and Ang-II significantly increased in diabetic model groups (P < 0.01) and body weight decreased (P < 0.05). The expression levels of LP and Ob-Rb increased and heart function decreased in diabetic model groups.
  
  
  CONCLUSIONS
  LP may be involved in the myocardial cell hypertrophy through the neuroendocrine system and associated with the JAK-STAT, Ras-Raf-MEK-MAPK and PI-3K signaling pathway, which provides a new concept for the pathogenesis of cardiac hypertrophy.
authors.affiliation: Department of Cardiology, Tianjin Fifth Central Hospital No. 41 Zhejiang Road, Tianjin 300450, P. R. China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Zuo
authors.affiliation: Department of Cardiology, Tianjin Fifth Central Hospital No. 41 Zhejiang Road, Tianjin 300450, P. R. China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Cardiology, Tianjin Fifth Central Hospital No. 41 Zhejiang Road, Tianjin 300450, P. R. China.
authors.forename: Liuying
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Cardiology, Tianjin Fifth Central Hospital No. 41 Zhejiang Road, Tianjin 300450, P. R. China.
authors.forename: Cuancuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Tianjin Fifth Central Hospital No. 41 Zhejiang Road, Tianjin 300450, P. R. China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Tianjin Fifth Central Hospital No. 41 Zhejiang Road, Tianjin 300450, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
keywords: Angiotensin II; Leptin; MAPK; Ob-Rb; streptozotocin; ventricular remodeling