title: Electrospun Collagen Fibers with Spatial Patterning of SDF1α for the Guidance of Neural Stem Cells.
abstract: Producing gradients of biological cues into nerve conduits is crucial for nerve guidance and regeneration. Herein, the fabrication of gradients of stromal cell-derived factor-1α (SDF1α) on electrospun collagen mats is reported using an electrohydrodynamic jet printing technique. The fabrication of various SDF1α gradated patterns on collagen fibrous mats is successfully demonstrated including shallow continuous gradient, steep continuous gradient, and step gradient by controlling the processing parameters. The SDF1α graded collagen scaffolds show a long-term stable gradient, as SDF1α is fused with a unique peptide of collagen binding domain (CBD), and CBD-SDF1α can specifically bind to the collagen mat. Such graded scaffolds exhibit sustained release of SDF1α. Further examination of neural stem cell (NSC) response to the CBD-SDF1α gradients with various patterns show that the NSCs can sense the CBD-SDF1α gradients, display a polarized morphology, and tend to migrate toward the region with a higher CBD-SDF1α content. The collagen mats with CBD-SDF1α gradients guide gradual distribution of NSCs, and NSC-differentiated neurons and astrocytes after seeding for 1 and 7 d. This new class of CBD-SDF1α gradient scaffolds can potentially be employed for guided nerve regeneration.
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.affiliation: Institute of Combined Injury State Key Laboratory of Trauma Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Bai
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.|Institute of Combined Injury State Key Laboratory of Trauma Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.|Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology Chinese Academy of Sciences, Beijing, 100080, China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D001253:Astrocytes; D002454:Cell Differentiation; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D058953:Neural Stem Cells / Q000378:metabolism*; D009474:Neurons; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D054377:Chemokine CXCL12; D003094:Collagen
keywords: collagen-binding domain; directed migration; neural stem cells; protein gradients