title: Quantitative analysis of the human T cell palmitome.
abstract: Palmitoylation is a reversible post-translational modification used to inducibly compartmentalize proteins in cellular membranes, affecting the function of receptors and intracellular signaling proteins. The identification of protein "palmitomes" in several cell lines raises the question to what extent this modification is conserved in primary cells. Here we use primary T cells with acyl-biotin exchange and quantitative mass spectrometry to identify a pool of proteins previously unreported as palmitoylated in vivo.
authors.affiliation: Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, 14195 Berlin, Germany.
authors.forename: Eliot
authors.initials: E
authors.lastname: Morrison
authors.affiliation: 1] Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, 14195 Berlin, Germany [2] Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
authors.forename: Benno
authors.initials: B
authors.lastname: Kuropka
authors.affiliation: Otto-von-Guericke University, Institute of Molecular and Clinical Immunology, Leipziger Strasse 44, 39120 Magdeburg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kliche
authors.affiliation: Heidelberg University Biochemistry Center (BZH)Im Neuenheimer Feld 328 69120 Heidelberg.
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.affiliation: Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, 14195 Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Freund
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D006801:Humans; D019169:Jurkat Cells; D054878:Lipoylation; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013601:T-Lymphocytes / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome