title: Differentiation of Normal and Cancer Cell Adhesion on Custom Designed Protein Nanopatterns.
abstract: Cell adhesion to the extracellular matrix is deregulated in metastasis. However, traditional surfaces used to study cell adhesion do not faithfully mimic the in vivo microenvironment. Electron beam lithography (EBL) is able to generate customized protein nanopatterns. Here, we used an EBL-based green lithography approach to fabricate homogeneous and gradient, single (fibronectin, K-casein) and double (fibronectin, laminin) active component protein nanopatterns with micrometer scale spacing to investigate differences in adhesion of breast cancer cells (BCC) and normal mammary epithelial cells (NMEC). Our results showed that as expected, in contrast to NMEC, BCC were plastic: they tolerated nonadhesion promoting regions, adapted to flow and exploited gradients better. In addition, the number of focal adhesions but not their area appeared to be the dominant parameter for regulation of cell adhesion. Our findings also demonstrated that custom designed protein nanopatterns, which can properly mimic the in vivo microenvironment, enable realistic distinction of normal and cancerous cell adhesion.
authors.affiliation: Department of Molecular Biology and Genetics, Izmir Institute of Technology, 35430 Urla/Izmir, Turkey.
authors.forename: Utku
authors.initials: U
authors.lastname: Horzum
authors.affiliation: Department of Molecular Biology and Genetics, Izmir Institute of Technology, 35430 Urla/Izmir, Turkey.
authors.forename: Berrin
authors.initials: B
authors.lastname: Ozdil
authors.affiliation: Department of Molecular Biology and Genetics, Izmir Institute of Technology, 35430 Urla/Izmir, Turkey.
authors.forename: Devrim
authors.initials: D
authors.lastname: Pesen-Okvur
mesh_terms: D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D002460:Cell Line; D045744:Cell Line, Tumor; D048430:Cell Shape; D004847:Epithelial Cells / Q000166:cytology; D004867:Equipment Design; D005260:Female; D005353:Fibronectins / Q000737:chemistry; D022001:Focal Adhesions / Q000473:pathology; D006801:Humans; D007797:Laminin / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology; D013499:Surface Properties
chemical_list: D005353:Fibronectins; D007797:Laminin
keywords: Focal adhesion; cancer; electron beam lithography; fibronectin; laminin; nanopattern