title: Long noncoding RNAs in hepatitis B virus-related hepatocellular carcinoma.
abstract: OBJECTIVE
  To study the expression of long noncoding RNAs (lncRNAs) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).
  
  
  METHODS
  The lncRNA profiles between HBV-related HCC tissues and corresponding normal liver tissues were generated using microarray analysis. Datasets were analyzed using multiple algorithms to depict alterations in gene expression on the basis of gene ontology (GO), pathway analysis, and lncRNA levels.
  
  
  RESULTS
  The microarray revealed that 1772 lncRNAs and 2508 mRNAs were differently expressed. The pathway analysis demonstrated that the cell cycle, cytokine-cytokine receptor interaction, chemokine signaling pathway, and phosphoinositide 3-kinase-protein kinase B signaling pathway may play important roles in HCC. Several GO terms, such as cell cycle, DNA replication, immune response, and signal transduction, were enriched in gene lists, suggesting a potential correlation with HBV-related HCC. The upregulated large intergenic noncoding RNA ULK4P2 was physically combined with enhancer of zeste homolog 2. Therefore, the lncRNAs may participate in regulating HBV-related HCC.
  
  
  CONCLUSIONS
  lncRNAs play important roles in HCC, future studies should verify whether large intergenic noncoding ULK4P2 functions by combining with enhancer of zeste homolog 2 in HCC.
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Yu
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Xu
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Jun-Jian
authors.initials: JJ
authors.lastname: Deng
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Han
authors.affiliation: Ting-Ting Yu, Xi-Ming Xu, Yi Hu, Jun-Jian Deng, Wei Ge, Na-Na Han, Mei-Xia Zhang, Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Mei-Xia
authors.initials: MX
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology; D016022:Case-Control Studies; D002472:Cell Transformation, Viral; D019295:Computational Biology; D030541:Databases, Genetic; D000071221:Enhancer of Zeste Homolog 2 Protein; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000472:pathogenicity*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D063151:Polycomb Repressive Complex 2 / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
keywords: Enhancer of zeste homolog 2; Hepatocellular carcinoma; Long noncoding RNAs; Microarray; ULK4P2