title: Molecular phylogenetics of the white-lipped peccary (Tayassu pecari) did not confirm morphological subspecies in northwestern South America.
abstract: We sequenced the mitochondrial DNA (mtDNA) control region of 59 peccaries (44 white-lipped peccaries, Tayassu pecari, and 15 collared peccaries, Pecari tajacu). We also genotyped 3 DNA microsatellites from 78 white-lipped peccaries representing the 4 putative morphological subspecies (i.e., spiradens, aequatoris, pecari, and albirostris) present in northwestern South America (i.e., Colombia, Ecuador, Peru, and Bolivia). Our results showed: 1) the estimated diversity of the mtDNA control region in the T. pecari population was extremely high, whereas the average genetic diversity for the microsatellites was medium to high and similar to that observed in European pig breeds; 2) there was no significant genetic heterogeneity among the quoted putative morphological subspecies at the mitochondrial marker, but we did detect significant (although relatively small) genetic heterogeneity using microsatellites, indicating that T. pecari albirostris is a uniquely differentiated group; and 3) the phylogenetic mtDNA trees showed that haplotypes were intermixed independent of their "a priori" subspecies classification. In addition, the microsatellite assignation analyses yielded low percentages of well-classified individuals when the analysis considered the geographic morphology of the subspecies. Thus, the molecular results do not support the putative morphological subspecies of T. pecari in northwestern South America. Finally, our results did not detect clear historical demographic changes using the mtDNA control region sequences. These genetic results are discussed in the context of the ecological and social characteristics of T. pecari.
authors.affiliation: Laboratorio de Genética de Poblaciones-Biología Evolutiva, Unidad de Genética, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia mruiz@javeriana.edu.co.
authors.forename: M
authors.initials: M
authors.lastname: Ruiz-García
authors.affiliation: Laboratorio de Genética de Poblaciones-Biología Evolutiva, Unidad de Genética, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia.
authors.forename: M
authors.initials: M
authors.lastname: Pinedo-Castro
authors.affiliation: Laboratorio de Genética de Poblaciones-Biología Evolutiva, Unidad de Genética, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia.
authors.forename: K
authors.initials: K
authors.lastname: Luengas-Villamil
authors.affiliation: Laboratorio de Genética de Poblaciones-Biología Evolutiva, Unidad de Genética, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia.
authors.forename: C
authors.initials: C
authors.lastname: Vergara
authors.affiliation: Instituto de Investigación de la Orinoquia Colombiana.
authors.forename: J A
authors.initials: JA
authors.lastname: Rodriguez
authors.affiliation: Biology Department, Penn State University-Fayette, Uniontown.
authors.forename: J M
authors.initials: JM
authors.lastname: Shostell
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes; D008322:Mammals / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D010802:Phylogeny; D013020:South America
chemical_list: D004272:DNA, Mitochondrial