title: Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation.
abstract: Estrogen plays an important role in inhibiting osteoclast differentiation and protecting against bone loss from osteoporosis, especially in postmenopausal women. However, the precise mechanisms underlying the effect of estrogen on osteoclasts are not well known. In the present study, we performed proteomics analysis and bioinformatics analysis to comprehensively compare the differential expression of proteins in receptor activator of nuclear factor-κB ligand RANKL-induced osteoclasts in the presence and absence of estrogen. We identified 6403 proteins, of which 124 were upregulated and 231 were downregulated by estrogen. Bioinformatics analysis showed that estrogen treatment interfered with 77 intracellular pathways, including both confirmed canonical and unconfirmed pathways of osteoclast formation. Our findings validate the inhibitory effect of estrogen on osteoclasts via the promotion of apoptosis and suppression of differentiation and polarization and suggest that estrogen might inhibit osteoclast formation via other pathways, which requires further investigation and verification.
authors.affiliation: Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Peifu
authors.initials: P
authors.lastname: Tang
authors.affiliation: National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, No. 5 Dongdansantiao, Dongcheng District, Beijing 100005, China.
authors.forename: Yanpan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Lihai
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, No. 5 Dongdansantiao, Dongcheng District, Beijing 100005, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000235:genetics / Q000503:physiopathology; D002454:Cell Differentiation / Q000235:genetics; D004967:Estrogens / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D010010:Osteoclasts / Q000378:metabolism* / Q000473:pathology; D010024:Osteoporosis / Q000378:metabolism / Q000503:physiopathology; D040901:Proteomics; D053245:RANK Ligand / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D004967:Estrogens; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse