title: [Effects of telmisartan on resistin expression in a rat model of nonalcoholic steatohepatitis and insulin resistance].
abstract: OBJECTIVE
  To investigate the effects of telmisartan on expression of resistin in serum and liver under conditions of nonalcoholic steatohepatitis (NASH) and insulin resistance using a rat model system.
  
  
  METHODS
  Forty-five male Sprague-Dawley rats were randomly divided into a normal control group (NC, n=10), a model control group (MC, n=15), a polyene phosphatidylcholine prevention group (PP, n=10), and a telmisartan prevention group (TP, n=10). The NC group was given a standard diet and the other groups were given a high-fat diet for 16 weeks in order to induce NASH. At the end of week 12, 5 rats in the MC group were sacrificed for pathology confirmation of the NASH model. At the end of week 12, the TP group was given telmisartan (8.0 mg/kg/d) and the PP group was given polyene phosphatidylcholine (8.4 mg/kg/d) for an additional 4 weeks by intragastric administration. At the end of week 16, all rats were sacrificed and body weights recorded. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglycerides (TG), resistin, insulin and fasting blood glucose were measured. The insulin resistance value, HOMA-IR, was assessed by homeostasis mode assessment. Liver expression of the resistin protein was detected by western blotting and of the resistin mRNA was detected by RT-PCR. The F test and LSD test were used for statistical analyses.
  
  
  RESULTS
  Compared to the NC group, the body weight and HOMA-IR of rats in the MC group were significantly increased (P<0.01). The levels of serum resistin, and of resistin protein and mRNA in liver, were significantly higher in the MC group than in the NC group of rats (all P less than 0.01). The body weight of rats in the TP group was significantly lower than those in the MC group (P<0.05). The levels of serrn resistin, resistin protein and mRNA in the liver, and insulin resistance were significantly lower in the TP group than in the MC group of rats (all P<0.01). The PP group did not show significant differences in any of these measures, except for loss of body weight (P<0.05).
  
  
  CONCLUSIONS
  Telmisartan elicits preventive and protective effects in a NASH rat model.Telmisartan may improve insulin resistance in NASH rats by decreasing the expression of serum resistin, and liver resistin protein and mRNA.
authors.affiliation: Department of Gastroenterology, Tianjin Fourth Central Hospital, Tianjin Medical University the Fourth Central Clinical College, Tianjin 300140, China.
authors.forename: Qiuzan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yingli
authors.initials: Y
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Xiuru
authors.initials: X
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Chenming
authors.initials: C
authors.lastname: Zhang
authors.forename: Bangmao
authors.initials: B
authors.lastname: Wang
mesh_terms: D000410:Alanine Transaminase; D000818:Animals; D001219:Aspartate Aminotransferases; D001562:Benzimidazoles; D001565:Benzoates; D002784:Cholesterol; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D007328:Insulin; D007333:Insulin Resistance; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D051381:Rats; D017207:Rats, Sprague-Dawley; D052243:Resistin; D000077333:Telmisartan; D014280:Triglycerides
chemical_list: D001562:Benzimidazoles; D001565:Benzoates; D007328:Insulin; D052243:Resistin; C499622:Retn protein, rat; D014280:Triglycerides; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000077333:Telmisartan