title: Polymorphisms in FTO and TCF7L2 genes of Euro-Brazilian women with gestational diabetes.
abstract: OBJECTIVE
  To investigate the association between fat mass and obesity-associated (FTO) gene polymorphisms rs8050136C>A and rs9939609T>A, and transcription factor 7-like 2 (TCF7L2) gene polymorphisms rs12255372G>T and rs7903146C>T, in a sample group of pregnant Euro-Brazilian women with or without gestational diabetes mellitus (GDM).
  
  
  METHODS
  Subjects were classified as either healthy pregnant control (n=200) or GDM (n=200) according to the 2010 criteria of the American Diabetes Association. The polymorphisms were genotyped using fluorescent probes (TaqMan®).
  
  
  RESULTS
  All groups were in the Hardy-Weinberg equilibrium. The genotype and allele frequencies of the examined polymorphisms did not exhibit significant difference (P>0.05) between the groups. In the healthy and GDM pregnant women groups, the A-allele frequencies (95% CI) of FTO polymorphisms rs8050136 and rs9939609 were 39% (34-44%); 38% (33-43%) and 40% (35-45%); 41% (36-46%), respectively; and the T-allele frequencies of TCF7L2 polymorphisms rs12255372 and rs7903146 were 30% (26-35%), 32% (27-37%) and 29% (25-34%), 36% (31-41%), respectively.
  
  
  CONCLUSIONS
  The examined polymorphisms were not associated with GDM in the Euro-Brazilian population studied.
authors.affiliation: Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil.
authors.forename: Sandra Fabrico
authors.initials: SF
authors.lastname: de Melo
authors.affiliation: Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil; Health and Biosciences School, Pontifical Catholic University of Parana, Curitiba, Parana, Brazil.
authors.forename: Henrique Ravanhol
authors.initials: HR
authors.lastname: Frigeri
authors.affiliation: Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil.
authors.forename: Izabella Castilhos Ribeiro
authors.initials: IC
authors.lastname: dos Santos-Weiss
authors.affiliation: Endocrinology and Metabolism Service (SEMPR), Clinical Hospital, Federal University of Parana, Brazil.
authors.forename: Rosângela Roginski
authors.initials: RR
authors.lastname: Réa
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Parana, Brazil.
authors.forename: Emanuel Maltempi
authors.initials: EM
authors.lastname: de Souza
authors.affiliation: Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil.
authors.forename: Dayane
authors.initials: D
authors.lastname: Alberton
authors.affiliation: Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil.
authors.forename: Fabiane
authors.initials: F
authors.lastname: Gomes de Moraes Rego
authors.affiliation: Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil. Electronic address: gpicheth@ufpr.br.
authors.forename: Geraldo
authors.initials: G
authors.lastname: Picheth
mesh_terms: D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D001938:Brazil; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics*; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: D011506:Proteins; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human
keywords: Case-controlled study; FTO; Gestational diabetes mellitus; Polymorphisms; SNP; TCF7L2