title: Hairy and Enhancer of Split 6 (Hes6) Deficiency in Mouse Impairs Neuroblast Differentiation in Dentate Gyrus Without Affecting Cell Proliferation and Integration into Mature Neurons.
abstract: Hes6 is a member of the hairy-enhancer of split homolog (Hes) family of transcription factors and interacts with other Hes family genes. During development, Hes genes are expressed in neural stem cells and progenitor cells. However, the role of Hes6 in adult hippocampal neurogenesis remains unclear. We therefore investigated the effects of Hes6 on adult hippocampal neurogenesis, by comparing Hes6 knockout and wild-type mice. To this end, we immunostained for markers of neural stem cells and progenitor cells (nestin), proliferating cells (Ki67), post-mitotic neuroblasts and immature neurons (doublecortin, DCX), mature neuronal cells (NeuN), and astrocyte (S100β). We also injected 5-bromo-2'-deoxyuridine (BrdU) to trace the fate of mitotic cells. Nestin- and Ki67-positive proliferating cells did now show any significant differences between wild and knockout groups. Hes6 knockout negatively affects neuroblast differentiation based on DCX immunohistochemistry. On the contrary, the ratio of the BrdU and NeuN double-positive cells did not show any significance, even though it was slightly higher in the knockout group. These results suggest that Hes6 is involved in the regulation of neuroblast differentiation during adult neurogenesis, but does not influence integration into mature neurons.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Nam
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Yo Na
authors.initials: YN
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Jong Whi
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Kyeong
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Seo Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Yeri
authors.initials: Y
authors.lastname: Son
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Life Sciences and Ewha Research Center for Systems Biology, Ewha Womans University, Seoul, 120-750, South Korea.
authors.forename: Jaesang
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biomedical Laboratory Science, College of Biomedical Sciences, Soonchunhyang University, Asan, 336-745, South Korea.
authors.forename: Sun Shin
authors.initials: SS
authors.lastname: Yi
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea. ysyoon@snu.ac.kr.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea. ysyoon@snu.ac.kr.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea. ysyoon@snu.ac.kr.
authors.forename: Yeo Sung
authors.initials: YS
authors.lastname: Yoon
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea. snumouse@snu.ac.kr.|BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea. snumouse@snu.ac.kr.|KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea. snumouse@snu.ac.kr.|Interdisciplinary Program for Bioinformatics, Program for Cancer Biology and BIO-MAX Institute, Seoul National University, Seoul, 151-742, South Korea. snumouse@snu.ac.kr.
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D001835:Body Weight; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005455:Fluorescent Antibody Technique; D005838:Genotype; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D064231:Nestin; D009479:Neuropeptides; D012097:Repressor Proteins; D001616:beta-Galactosidase; C000714847:Dcx protein, mouse; C486570:Hes6 protein, mouse
keywords: Adult neurogenesis; Hes6; Hippocampus; Neuronal differentiation