title: Specific Proteins in Nontuberculous Mycobacteria: New Potential Tools.
abstract: Nontuberculous mycobacteria (NTM) have been isolated from water, soil, air, food, protozoa, plants, animals, and humans. Although most NTM are saprophytes, approximately one-third of NTM have been associated with human diseases. In this study, we did a comparative proteomic analysis among five NTM strains isolated from several sources. There were different numbers of protein spots from M. gordonae (1,264), M. nonchromogenicum type I (894), M. nonchromogenicum type II (935), M. peregrinum (806), and M. scrofulaceum/Mycobacterium mantenii (1,486) strains, respectively. We identified 141 proteins common to all strains and specific proteins to each NTM strain. A total of 23 proteins were selected for its identification. Two of the common proteins identified (short-chain dehydrogenase/reductase SDR and diguanylate cyclase) did not align with M. tuberculosis complex protein sequences, which suggest that these proteins are found only in the NTM strains. Some of the proteins identified as common to all strains can be used as markers of NTM exposure and for the development of new diagnostic tools. Additionally, the specific proteins to NTM strains identified may represent potential candidates for the diagnosis of diseases caused by these mycobacteria.
authors.affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Orduña
authors.affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.
authors.forename: Antonia I
authors.initials: AI
authors.lastname: Castillo-Rodal
authors.affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.
authors.forename: Martha E
authors.initials: ME
authors.lastname: Mercado
authors.affiliation: División de Investigación Clínica, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.
authors.forename: Samuel
authors.initials: S
authors.lastname: Ponce de León
authors.affiliation: División de Investigación Clínica, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: López-Vidal
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000235:genetics* / Q000473:pathology; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins