title: SWP73 Subunits of Arabidopsis SWI/SNF Chromatin Remodeling Complexes Play Distinct Roles in Leaf and Flower Development.
abstract: Arabidopsis thaliana SWP73A and SWP73B are homologs of mammalian BRAHMA-associated factors (BAF60s) that tether SWITCH/SUCROSE NONFERMENTING chromatin remodeling complexes to transcription factors of genes regulating various cell differentiation pathways. Here, we show that Arabidopsis thaliana SWP73s modulate several important developmental pathways. While undergoing normal vegetative development, swp73a mutants display reduced expression of FLOWERING LOCUS C and early flowering in short days. By contrast, swp73b mutants are characterized by retarded growth, severe defects in leaf and flower development, delayed flowering, and male sterility. MNase-Seq, transcript profiling, and ChIP-Seq studies demonstrate that SWP73B binds the promoters of ASYMMETRIC LEAVES1 and 2, KANADI1 and 3, and YABBY2, 3, and 5 genes, which regulate leaf development and show coordinately altered transcription in swp73b plants. Lack of SWP73B alters the expression patterns of APETALA1, APETALA3, and the MADS box gene AGL24, whereas other floral organ identity genes show reduced expression correlating with defects in flower development. Consistently, SWP73B binds to the promoter regions of APETALA1 and 3, SEPALLATA3, LEAFY, UNUSUAL FLORAL ORGANS, TERMINAL FLOWER1, AGAMOUS-LIKE24, and SUPPRESSOR OF CONSTANS OVEREXPRESSION1 genes, and the swp73b mutation alters nucleosome occupancy on most of these loci. In conclusion, SWP73B acts as important modulator of major developmental pathways, while SWP73A functions in flowering time control.
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland.
authors.forename: Sebastian P
authors.initials: SP
authors.lastname: Sacharowski
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland.
authors.forename: Dominika M
authors.initials: DM
authors.lastname: Gratkowska
authors.affiliation: Marie Curie Memorial Cancer Center, 02-781 Warsaw, Poland.
authors.forename: Elzbieta A
authors.initials: EA
authors.lastname: Sarnowska
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Kondrak
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
authors.forename: Iga
authors.initials: I
authors.lastname: Jancewicz
authors.affiliation: Max-Planck Institut für Pflanzenzüchtungsforschung, D-50829 Köln, Germany.
authors.forename: Aimone
authors.initials: A
authors.lastname: Porri
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland Universtity of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology, Department of Plant Molecular Biology, 02-106 Warsaw, Poland.
authors.forename: Ernest
authors.initials: E
authors.lastname: Bucior
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland Universtity of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology, Department of Plant Molecular Biology, 02-106 Warsaw, Poland.
authors.forename: Anna T
authors.initials: AT
authors.lastname: Rolicka
authors.affiliation: Max-Planck Institut für Pflanzenzüchtungsforschung, D-50829 Köln, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Franzen
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Kowalczyk
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawlikowska
authors.affiliation: Max Planck Genome Centre Cologne, D-50820 Köln, Germany.
authors.forename: Bruno
authors.initials: B
authors.lastname: Huettel
authors.affiliation: Max-Planck Institut für Pflanzenzüchtungsforschung, D-50829 Köln, Germany.
authors.forename: Stefano
authors.initials: S
authors.lastname: Torti
authors.affiliation: Max-Planck Institut für Pflanzenzüchtungsforschung, D-50829 Köln, Germany.
authors.forename: Elmon
authors.initials: E
authors.lastname: Schmelzer
authors.affiliation: Max-Planck Institut für Pflanzenzüchtungsforschung, D-50829 Köln, Germany.
authors.forename: George
authors.initials: G
authors.lastname: Coupland
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland Universtity of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology, Department of Plant Molecular Biology, 02-106 Warsaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Jerzmanowski
authors.affiliation: Max-Planck Institut für Pflanzenzüchtungsforschung, D-50829 Köln, Germany Institute of Plant Biology, Biological Research Center of Hungarian Academy, H-6724 Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Koncz
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Department of Protein Biosynthesis, 02-106 Warsaw, Poland tsarn@ibb.waw.pl.
authors.forename: Tomasz J
authors.identifier: http://orcid.org/0000-0002-3805-2039
authors.initials: TJ
authors.lastname: Sarnowski
mesh_terms: D017360:Arabidopsis / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008836:Micrococcal Nuclease / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009707:Nucleosomes / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes; D021122:Protein Subunits; D012333:RNA, Messenger; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; D008836:Micrococcal Nuclease