title: Impact of Implementation of an Automated Liquid Culture System on Diagnosis of Tuberculous Pleurisy.
abstract: This study was conducted to evaluate the impact of implementation of an automated liquid culture system on the diagnosis of tuberculous pleurisy in an HIV-uninfected patient population. We retrospectively compared the culture yield, time to positivity, and contamination rate of pleural effusion samples in the BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) and Ogawa media among patients with tuberculous pleurisy. Out of 104 effusion samples, 43 (41.3%) were culture positive on either the MGIT or the Ogawa media. The culture yield of MGIT was higher (40.4%, 42/104) than that of Ogawa media (18.3%, 19/104) (P<0.001). One of the samples was positive only on the Ogawa medium. The median time to positivity was faster in the MGIT (18 days, range 8-32 days) than in the Ogawa media (37 days, range 20-59 days) (P<0.001). No contamination or growth of nontuberculous mycobacterium was observed on either of the culture media. In conclusion, the automated liquid culture system could provide approximately twice as high yields and fast results in effusion culture, compared to solid media. Supplemental solid media may have a limited impact on maximizing sensitivity in effusion culture; however, further studies are required.
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Byung Hee
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Seong Hoon
authors.initials: SH
authors.lastname: Yoon
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Hye Ju
authors.initials: HJ
authors.lastname: Yeo
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Dong Wan
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Seung Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Woo Hyun
authors.initials: WH
authors.lastname: Cho
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Yun Seong
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea. ; Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea.
authors.forename: Doosoo
authors.initials: D
authors.lastname: Jeon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D057205:Automation, Laboratory / Q000379:methods*; D018929:Cell Culture Techniques; D003470:Culture Media / Q000145:classification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis; D010994:Pleura / Q000382:microbiology / Q000473:pathology; D012189:Retrospective Studies; D013183:Sputum / Q000382:microbiology*; D014396:Tuberculosis, Pleural / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D003470:Culture Media
keywords: Culture Media; Pleural Effusion; Pleurisy; Tuberculosis