title: Overexpression of SULT2B1b is an independent prognostic indicator and promotes cell growth and invasion in colorectal carcinoma.
abstract: Aberrant expression of cytosolic sulfotransferase 2B1b (SULT2B1b) has been reported in several human malignancies. However, the expression pattern and clinical significance of SULT2B1b in colorectal carcinoma (CRC) remains unknown. Real-time quantitative PCR, western blot, and immunohistochemistry analyses were used to determine SULT2B1b expression in CRC clinical samples and CRC-derived cell lines. Kaplan-Meier and Cox proportional regression analyses were used to evaluate the association between SULT2B1b expression and patient survival in two independent cohorts of 485 patients with CRC. Gain- and loss-of-function approaches were employed to investigate the role of SULT2B1b in regulation of CRC cell growth and invasion. We found that SULT2B1b expression was frequently upregulated in CRC clinical samples and CRC-derived cell lines and was significantly correlated with lymph node metastasis and TNM stage in both the training and validation cohorts. Patients with higher intratumoral SULT2B1b expression had a significantly shorter disease-specific survival (DSS) and disease-free survival (DFS) than those with lower expression. Importantly, increased expression of SULT2B1b significantly predicted poor DSS and DFS and was an independent unfavorable prognostic indicator for stage II patients in both cohorts. Functional studies revealed that overexpression of SULT2B1b promoted CRC cell growth and invasion in vitro. Conversely, knockdown of SULT2B1b inhibited these processes. In conclusion, our findings suggest that SULT2B1b expression correlates with disease progression and metastasis and may serve as a novel prognostic biomarker and potential therapeutic target for patients with CRC.
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.|Department of Clinical Laboratory, 150th Hospital of PLA, Luoyang, China.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Feng
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Zhai
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Chen-Ye
authors.initials: CY
authors.lastname: Qi
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Fu
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.|Department of Pathology, 150th Hospital of PLA, Luoyang, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Ye
authors.affiliation: Department of Gastrointestine Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Cai
authors.affiliation: Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Gao
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D015238:Sulfotransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015238:Sulfotransferases; C433248:SULT2B1 protein, human