title: Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics.
abstract: Ultraviolet (UV) radiation causes cellular DNA damage, among which cyclobutane pyrimidine dimers (CPDs) are responsible for a variety of genetic mutations. Although several approaches have been developed for detection of CPDs, conventional methods require time-consuming steps. Aquaphotomics, a new approach based on near-infrared spectroscopy (NIRS) and multivariate analysis that determines interactions between water and other components of the solution, has become an effective method for qualitative and quantitative parameters measurement in the solutions. NIR spectral patterns of UVC-irradiated and nonirradiated DNA solutions were evaluated using aquaphotomics for detection of UV-induced CPDs. Groups of UV-irradiated and nonirradiated DNA samples were classified (87.5% accuracy) by soft independent modeling of class analogy (SIMCA). A precise regression model calculated from NIR water spectral patterns based on UVC doses (r Val = 0.9457) and the concentration of cis-syn cyclobutane thymine dimers (cis-syn T<>Ts; r Val = 0.9993) was developed using partial least squares regression (PLSR), while taking advantage of water spectral patterns, particularly around 1400-1500 nm. Our results suggested that, in contrast to DNA, the formation of cis-syn T<>Ts increased the strongly hydrogen bonded water. Additionally, NIRS could qualitatively and quantitatively detect cis-syn T<>Ts in isolated DNA aqueous solutions upon UVC exposure.
authors.affiliation: Division of Dermatology, Department of Internal Related, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Goto
authors.affiliation: 1] Biomeasurement Technology Laboratory, Graduate School of Agriculture, Kobe University, Kobe 657-8501, Japan [2] Institute of Food and Agricultural Product Qualification, Faculty of Agricultural and Environmental Sciences, Kaposvar University, Kaposvar 7401, Hungary.
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Bazar
authors.affiliation: 1] Biomeasurement Technology Laboratory, Graduate School of Agriculture, Kobe University, Kobe 657-8501, Japan [2] Department of Physics and Control, Corvinus University of Budapest, Budapest 1118, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kovacs
authors.affiliation: Division of Dermatology, Department of Internal Related, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kunisada
authors.affiliation: Biomeasurement Technology Laboratory, Graduate School of Agriculture, Kobe University, Kobe 657-8501, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8501, Japan.
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kizaki
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: Biomeasurement Technology Laboratory, Graduate School of Agriculture, Kobe University, Kobe 657-8501, Japan.
authors.forename: Roumiana
authors.initials: R
authors.lastname: Tsenkova
authors.affiliation: Division of Dermatology, Department of Internal Related, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Nishigori
mesh_terms: D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D016296:Mutagenesis / Q000528:radiation effects; D009154:Mutation / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000302:isolation & purification* / Q000528:radiation effects; D019265:Spectroscopy, Near-Infrared; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; D004247:DNA