title: Expression, regulation and function of Hmgn3 during decidualization in mice.
abstract: Although Hmgn3 is involved in the regulation of development and cellular differentiation, its physiological roles on decidualization are still unknown. Here we showed that Hmgn3 was highly expressed in the decidua and decidualizing stromal cells. Overexpression of Hmgn3 variants, Hmgn3a or Hmgn3b, enhanced the expression of decidualization markers Prl8a2 and Prl3c1, whereas inhibition of Hmgn3 reduced their expression. Hmgn3 could mediate the effects of Hoxa10 and cAMP on the expression of Prl8a2 and Prl3c1. Further study found that Hmgn3 directed the process of decidualization through influencing the expression of Hand2. Progesterone could induce the expression of Hmgn3 in the ovariectomized mouse uterus, uterine epithelial cells and stromal cells. Knockdown of Hoxa10 with siRNA alleviated the induction of progesterone and cAMP on Hmgn3 expression. Simultaneously, siRNA-mediated down-regulation of Hmgn3 in the uterine stromal cells could attenuate the effects of progesterone, cAMP and Hoxa10 on the expression of Hand2. Collectively, Hmgn3 may play an important role during mouse decidualization.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, PR China.
authors.forename: Dang-Dang
authors.initials: DD
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, PR China.
authors.forename: Chuan-Hui
authors.initials: CH
authors.lastname: Guo
authors.affiliation: College of Clinical Medicine, Jilin University, Changchun, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yue
authors.affiliation: Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences, Changchun, PR China.
authors.forename: Cui-Cui
authors.initials: CC
authors.lastname: Duan
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, PR China.
authors.forename: Zhan-Qing
authors.initials: ZQ
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, PR China.
authors.forename: Hang
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, PR China. Electronic address: guobin79@jlu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, PR China.
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Yue
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D024001:HMGN Proteins / Q000096:biosynthesis*; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis; D051379:Mice; D011247:Pregnancy / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D020033:Protein Isoforms / Q000096:biosynthesis
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D024001:HMGN Proteins; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D011374:Progesterone; D020033:Protein Isoforms; C532059:HMGN3 protein, mouse; C498766:Hand2 protein, mouse; C092119:Hoxa10 protein, mouse
keywords: Decidualization; Hmgn3; Mouse; Stromal cell; Uterus