title: [Effect of Vascular Cell Adhesion Molecule -1 Overexpression on Adipogenic Differentiation of Murine Mesenchymal Stem Cells and Its Mechanism].
abstract: OBJECTIVE
  To investigate the effect of vascular cell adhesion molecule-1 (VCAM-1) gene overexpression on adipogenic differentiation of mouse mesenchymal stem cells(MSC) and explore its molecular mechanism.
  
  
  METHODS
  VCAM-1 overexpression MSC (MIGR1-VCAM-1/MSC) and the empty plasmid transfection MSC (MIGR1/MSC) were induced to adipogenic differentiation, oil-red-O staining and real-time PCR were used to detect the adipogenic differentiation ability and the mRNA expression level of key transcription factors C/EBP α and PPAR γ. The activation of P38, ERK and JNK pathways were analyzed by Western blot. Furthermore, the specific chemical inhibitors of MAPK pathway (SB203580, PD98059 and JNK inhibitor II) were added to the induced culture system and the alteration of the MSC adipogenic differentiation ability were evaluated.
  
  
  RESULTS
  no matter in self or induced differentiation groups, the lipid droplets in MIGR1-VCAM-1/MSC became larger, the amount of adipocyte increased than that in MIGR1/MSC (P<0.01), the mRNA expression level of C/EBPα and PPARγ were upregulated, and JNK pathway were down-regulated while the P38 and ERK pathway were significantly up-regulated. The inhibition of JNK pathway of MIGR1-VCAM-1/MSC could lead to increased mRNA expression level of C/EBP α and PPAR γ, the amount of adipocytes increased (P<0.01), however, the inhibition of the P38 and ERK pathway of MIGR1-VCAM-1/MSC could lead to decreased mRNA expression level of C/EBP α and PPAR γ, and the lipid droplets and the number of adipocytes became smaller and less.
  
  
  CONCLUSIONS
  The overexpression of VCAM-1 may promote MSC to differentiate into adipocytes through inhibiting JNK signaling pathway, activating P38 and ERK pathways.
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Thyroid and Breast Surgery, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Pediatrics, General Hospital of Tianjin Medical University, Tianjin 30052, China.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Ni
authors.affiliation: Department of Pediatrics, General Hospital of Tianjin Medical University, Tianjin 30052, China.
authors.forename: Shi-Feng
authors.initials: SF
authors.lastname: Ma
authors.affiliation: Department of Postgraduate, Hebei North College, Zhangjiakou 07500, Heibei Province China.
authors.forename: Xiu-Hui
authors.initials: XH
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, General Hospital of Tianjin Medical University, Tianjin 30052, China. E-mail: rxzheng@hotmail.com.
authors.forename: Rong-Xiu
authors.initials: RX
authors.lastname: Zheng
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850. E-mail: zhangyi612@hotmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha; D002454:Cell Differentiation; D015536:Down-Regulation; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells; D051379:Mice; D047495:PPAR gamma; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D019010:Vascular Cell Adhesion Molecule-1