title: RNA-Seq-based transcriptome analysis of Korean rose bitterling (Rhodeus uyekii) exposed to synthetic estrogen 17-α-ethinylestradiol (EE2).
abstract: The potential impact of natural and synthetic estrogens on aquatic ecosystems has become a subject of great interest in recent years. One synthetic estrogen, 17-alpha-ethinylestradiol (EE2), is present in municipal sewage discharges and causes gonad alterations in various fish species. To understand the possible damage caused by EE2, male Rhodeus uyekii were exposed to 100 ng/L EE2 for 7 days. RNA-Seq was performed to assess the effects of EE2 on gene expression in hepatic and skin tissues. The analysis revealed that EE2 induced the expression of various genes, including sex hormone genes, anti-Mullerian hormone, vitellogenin, and estrogen receptor alpha; cancer genes, breast cancer anti-estrogen resistance protein 3, caveolin 2, and Smad2; and apoptotic genes, p53, Bcl-2, TNF-α, and WDR36. These results suggest that the synthetic estrogen EE2 disturbs the endocrine system and regulates both carcinogenic and apoptotic gene expressions in R. uyekii.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea. Electronic address: heejkong@korea.kr.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.affiliation: All Bio Technology Co., LTD, Internet Business Incubation Center, Mokweon University, Daejeon 302-729, Republic of Korea.
authors.forename: Il Kyu
authors.initials: IK
authors.lastname: Lee
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea.
authors.forename: Julan
authors.initials: J
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea.
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: All Bio Technology Co., LTD, Internet Business Incubation Center, Mokweon University, Daejeon 302-729, Republic of Korea.
authors.forename: Wang Sik
authors.initials: WS
authors.lastname: Cho
authors.affiliation: All Bio Technology Co., LTD, Internet Business Incubation Center, Mokweon University, Daejeon 302-729, Republic of Korea.
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea.
authors.forename: Jung Youn
authors.initials: JY
authors.lastname: Park
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea.
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
mesh_terms: D000818:Animals; D003531:Cypriniformes / Q000378:metabolism*; D004967:Estrogens / Q000737:chemistry / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000737:chemistry / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D059467:Transcriptome; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D004967:Estrogens; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol