title: Effect of Ginkgo biloba extract on apoptosis of brain tissues in rats with acute cerebral infarction and related gene expression.
abstract: We investigated the effect of Ginkgo biloba extract on apoptosis of brain tissues in rats with acute cerebral infarction and apoptosis-related gene expression. Rat models of acute cerebral infarction were constructed using the suture method, and randomly divided into the control group, model, and treatment groups. In the treatment group, 4 mg/kg G. biloba extract was intravenously injected into the rat tail vein. Phosphate-buffered saline solution was injected in the model group. Seventy-two hours after treatment, rats were euthanized, and brain tissues were removed to analyze the changes in caspase-3, B-cell lymphoma 2 (Bcl-2), and Bcl-2-associated X protein (Bax) mRNA and protein levels, and variation in brain tissue cells' apoptosis indices was measured. Compared with the control group, the model and treatment groups showed significantly upregulated caspase-3, Bcl-2, and Bax mRNA and protein levels in brain tissues, but remarkably downregulated Bcl-2 mRNA and protein levels (P < 0.05). After treatment, in treatment group brain tissues, caspase-3 and Bax mRNA and protein levels were significantly lower than those in the model group, while Bcl-2 mRNA and protein levels were higher than that in the model group (P < 0.05). The model and treatment groups showed increased cell apoptosis indices of brain tissues compared to the control group; after treatment, the apoptosis index in the treatment group was significantly downregulated compared with that in the model group (P < 0.05). In conclusion, G. biloba extract significantly reduced apoptosis in rat brain tissue cells with acute cerebral infarction and thus protected brain tissues.
authors.affiliation: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, China.
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, China.
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, China yongchencn@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D020441:Ginkgo biloba / Q000737:chemistry; D006801:Humans; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D053148:Caspase 3