title: Comparison of in vitro methods for the production of Paenibacillus larvae endospores.
abstract: Paenibacillus larvae endospores are the infectious particles of the honey bee brood disease, American Foulbrood. We demonstrate that our previously published protocol (Alvarado et al., 2013) consistently yields higher numbers and purer preparations of P. larvae endospores, than previously described protocols, regardless of the strain tested (B-3650, B-3554 or B-3685).
authors.affiliation: School of Life Sciences, 4505 South Maryland Parkway, Bldg WHI, M/S 4004, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: alvara95@unlv.nevada.edu.
authors.forename: Israel
authors.initials: I
authors.lastname: Alvarado
authors.affiliation: School of Life Sciences, 4505 South Maryland Parkway, Bldg WHI, M/S 4004, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: melekoni@nsf.gov.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Elekonich
authors.affiliation: Department of Chemistry, 4505 South Maryland Parkway, Bldg CHE, M/S 4003, University of Nevada, Las Vegas, Las Vegas, NV 89154-4003, United States. Electronic address: ernesto.abelsantos@unlv.edu.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Abel-Santos
authors.affiliation: School of Life Sciences, 4505 South Maryland Parkway, Bldg WHI, M/S 4004, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States. Electronic address: helen.wing@unlv.edu.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Wing
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology*; D056507:Paenibacillus / Q000502:physiology*; D013171:Spores, Bacterial / Q000254:growth & development* / Q000648:ultrastructure
keywords: American Foulbrood disease; Honey bee; Paenibacillus larvae; Spore production