title: Oxidative stress triggers lipid droplet accumulation in primary cultured hepatocytes by activating fatty acid synthesis.
abstract: Despite the impaired intestinal lipid absorption and low level of visceral fat, the Sod1-deficient mouse is susceptible to developing liver steatosis. To gain insights into the mechanism responsible for this abnormal lipid metabolism, we analyzed primary cultured hepatocytes obtained from Sod1-deficient and wild-type mice. Lipid droplets began to accumulate in the cultured hepatocytes and was further increased by a Sod1 deficiency. Levels of enzymes involved in lipogenesis were elevated. It thus appears that lipogenesis is activated by oxidative stress, which is more prominent in the case of Sod1 deficiency, and appears to participate in liver steatosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Medical Sciences, Yamagata University, Japan.
authors.forename: Jaeyong
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Medical Sciences, Yamagata University, Japan.
authors.forename: Takujiro
authors.initials: T
authors.lastname: Homma
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Medical Sciences, Yamagata University, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kurahashi
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Medical Sciences, Yamagata University, Japan.
authors.forename: Eun Sil
authors.initials: ES
authors.lastname: Kang
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Medical Sciences, Yamagata University, Japan. Electronic address: jfujii@med.id.yamagata-u.ac.jp.
authors.forename: Junichi
authors.initials: J
authors.lastname: Fujii
mesh_terms: D000818:Animals; D005227:Fatty Acids / Q000096:biosynthesis*; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D007408:Intestinal Absorption; D066292:Lipid Droplets / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D050155:Lipogenesis / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018384:Oxidative Stress; D061251:Primary Cell Culture; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics*; D000072105:Superoxide Dismutase-1
chemical_list: D005227:Fatty Acids; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1
keywords: Lipogenesis; Liver steatosis; Oxidative stress; Superoxide dismutase