title: The Volumetric Diversity of Misfolded Prion Protein Oligomers Revealed by Pressure Dissociation.
abstract: Protein oligomerization has been associated with a wide range of diseases. High pressure approaches offer a powerful tool for deciphering the underlying molecular mechanisms by revealing volume changes associated with the misfolding and assembly reactions. We applied high pressure to induce conformational changes in three distinct β-sheet-rich oligomers of the prion protein PrP, a protein characterized by a variety of infectious quaternary structures that can propagate stably and faithfully and cause diseases with specific phenotypic traits. We show that pressure induces dissociation of the oligomers and leads to a lower volume monomeric PrP state that refolds into the native conformation after pressure release. By measuring the different pressure and temperature sensitivity of the tested PrP oligomers, we demonstrate significantly different void volumes in their quaternary structure. In addition, by focusing on the kinetic and energetic behavior of the pressure-induced dissociation of one specific PrP oligomer, we reveal a large negative activation volume and an increase in both apparent activation enthalpy and entropy. This suggests a transition state ensemble that is less structured and significantly more hydrated than the oligomeric state. Finally, we found that site-specific fluorescent labeling allows monitoring of the transient population of a kinetic intermediate in the dissociation reaction. Our results indicate that defects in atomic packing may deserve consideration as a new factor that influences differences between PrP assemblies and that could be relevant also for explaining the origin of prion strains.
authors.affiliation: From the Institut National de la Recherche Agronomique, UR892, Virologie Immunologie Moléculaires, Domaine de Vilvert, F-78350 Jouy-en-Josas, France and joan.torrent@inserm.fr.
authors.forename: Joan
authors.initials: J
authors.lastname: Torrent
authors.affiliation: the Institut National de la Recherche Agronomique, UMR1208, Ingénierie des Agropolymères et Technologies Emergentes, Université Montpellier, F-34095 Montpellier, France.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Lange
authors.affiliation: From the Institut National de la Recherche Agronomique, UR892, Virologie Immunologie Moléculaires, Domaine de Vilvert, F-78350 Jouy-en-Josas, France and.
authors.forename: Human
authors.initials: H
authors.lastname: Rezaei
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D011312:Pressure; D011328:Prions / Q000737:chemistry*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D012756:Sheep
chemical_list: D001704:Biopolymers; D011328:Prions; D011994:Recombinant Proteins
keywords: oligomer; pressure; prion; protein misfolding; protein self-assembly; protein stability