title: Activity of matrix metalloproteinases 2 and 9 in cultured rabbit corneal epithelium cells stimulated by tumor necrosis factor alpha.
abstract: We studied the activity of matrix metalloproteinases (MMP) 2 and 9 generated by cultured rabbit corneal epithelium cells that had been stimulated with tumor necrosis factor alpha (TNF-α), to investigate the possible regulative mechanisms of MMP-2/9 and their potential effect on corneal inflammatory diseases. The rabbit corneal epithelium cells were cultured in vitro and incubated with different concentrations of TNF-α (0, 1, 10, and 100 ng/mL) for 24 h. The activity of MMP-2/9 was examined using gelatin zymography. The results were analyzed by computer image analysis and statistical tests. TNF-α stimulated the secretion of MMP-2/9 in a dose-dependent manner, and MMP-2 was activated by TNF-α. Inflammatory factors such as TNF-α can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases, and could play an important role in the prevention and treatment of corneal inflammatory diseases.
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Wu
authors.affiliation: Department of Gastrointestinal Surgery, Jinzhou Central Hospital, Jinzhou, Hubei, China.
authors.forename: Z-L
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Jinzhou Central Hospital, Jinzhou, Hubei, China.
authors.forename: S-W
authors.initials: SW
authors.lastname: Nie
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: J
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China 417955104@qq.com.
authors.forename: Y-N
authors.initials: YN
authors.lastname: Yang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003316:Corneal Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D019573:Epithelium, Corneal / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9