title: Intraspecies Interaction of Fusarium graminearum Contributes to Reduced Toxin Production and Virulence.
abstract: Fusarium graminearum is a pathogenic fungus that causes Fusarium head blight in wheat and lowers the yield and quality of grains by contamination with the trichothecene mycotoxin deoxynivalenol. The fungi coexist and interact with several different fusaria as well as other plant pathogenic fungi and bacteria in the field. In Canada, F. graminearum exists as two main trichothecene chemotypes: 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol. To understand the potential interactions between two isolates of these chemotypes, we conducted coinoculation studies both in culture and in planta. The studies showed that intraspecies interaction reduces trichothecene yield in culture and disease symptoms in wheat. To elucidate the genes involved in the intraspecies interaction, expression profiling was performed on RNA samples isolated from coinoculated cultures, and potential genes were identified by using the genome sequences of the respective isolates.
authors.affiliation: 1 Eastern Cereal and Oilseed Research Centre, 960 Carling, Agriculture and Agri-Food Canada, Ottawa K1A 0C6, Canada;|2 Department of Biology, 1125 Colonel By, Carleton University, Ottawa K1S 5B6, Canada.
authors.forename: Sean
authors.initials: S
authors.lastname: Walkowiak
authors.affiliation: 1 Eastern Cereal and Oilseed Research Centre, 960 Carling, Agriculture and Agri-Food Canada, Ottawa K1A 0C6, Canada;|2 Department of Biology, 1125 Colonel By, Carleton University, Ottawa K1S 5B6, Canada.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Bonner
authors.affiliation: 1 Eastern Cereal and Oilseed Research Centre, 960 Carling, Agriculture and Agri-Food Canada, Ottawa K1A 0C6, Canada;
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: 1 Eastern Cereal and Oilseed Research Centre, 960 Carling, Agriculture and Agri-Food Canada, Ottawa K1A 0C6, Canada;
authors.forename: Barbara
authors.initials: B
authors.lastname: Blackwell
authors.affiliation: 2 Department of Biology, 1125 Colonel By, Carleton University, Ottawa K1S 5B6, Canada.
authors.forename: Owen
authors.initials: O
authors.lastname: Rowland
authors.affiliation: 1 Eastern Cereal and Oilseed Research Centre, 960 Carling, Agriculture and Agri-Food Canada, Ottawa K1A 0C6, Canada;
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Subramaniam
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal / Q000235:genetics; D054884:Host-Pathogen Interactions; D056265:Microbial Interactions / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D059467:Transcriptome; D014255:Trichothecenes / Q000096:biosynthesis*; D014908:Triticum / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D014255:Trichothecenes; C043247:3-acetyldeoxynivalenol; C046760:15-acetyldeoxynivalenol