title: Comparative Transcriptome Analysis of the Cosmopolitan Marine Fungus Corollospora maritima Under Two Physiological Conditions.
abstract: Marine sandy beaches represent dynamic environments often subject to harsh conditions and climate fluctuations, where natural and anthropogenic inputs of freshwater from fluvial and pluvial sources alter salinity, which has been recognized as a key variable affecting the distribution of aquatic organisms and influencing critical physiological processes. The marine arenicolous fungus Corollospora maritima is a worldwide-distributed saprobe that has been reported to present tolerance to freshwater. Here, we present a transcriptome analysis that will provide the first insight of the genomic content for this fungus and a gene expression comparison between two different salinity conditions. We also identified genes that are candidates for being differentially expressed in response to environmental variations on salinity during the fungal growth. The de novo reconstruction of C. maritima transcriptome Illumina sequencing provided a total of 14,530 transcripts (16 megabases). The comparison between the two growth conditions rendered 103 genes specifically overexpressed in seawater, and 132 genes specifically up-regulated under freshwater. Using fungal isolates collected from different beaches, the specific environmental regulation of particular transcript differential expression was confirmed by RT-qPCR. To our knowledge, this is the first analysis that explores the marine fungus C. maritima molecular responses to overcome freshwater stress, and these data could shed light to understand the fungal adaptation and plasticity mechanisms to the marine habitat.
authors.affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Distrito Federal, México 04510 pvelezaguilar@gmail.com cesy@unam.mx.
authors.forename: Patricia
authors.initials: P
authors.lastname: Velez
authors.affiliation: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Distrito Federal, México 04510.
authors.forename: Naholi D
authors.initials: ND
authors.lastname: Alejandri-Ramírez
authors.affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Distrito Federal, México 04510.
authors.forename: María C
authors.initials: MC
authors.lastname: González
authors.affiliation: Unidad de Secuenciación Masiva y Bioinformática Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México 62210.
authors.forename: Karel J
authors.initials: KJ
authors.lastname: Estrada
authors.affiliation: Unidad de Secuenciación Masiva y Bioinformática Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México 62210.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sanchez-Flores
authors.affiliation: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Distrito Federal, México 04510 pvelezaguilar@gmail.com cesy@unam.mx.
authors.forename: Tzvetanka D
authors.initials: TD
authors.lastname: Dinkova
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D001203:Ascomycota / Q000145:classification / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D015966:Gene Expression Regulation, Fungal; D023281:Genomics / Q000379:methods; D010802:Phylogeny; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000235:genetics; D059467:Transcriptome
keywords: Halosphaeriaceae; environmental stress; fungal transcriptomics; intertidal zone; sandy seashore