title: [Molecular genetic characterization of the Far Eastern trematode Skrjabino lecithum spasskii Belous, 1954 (Digenea: Haploporidae)), a parasite of mullets].
abstract: Intraspecific genetic differentiation of the trematode Skrjabinolecithum spasskii and its phylogenetic relationships with other species of the family Haploporidae were studied by comparing the nucleotide sequences of a part of the 28S rRNA gene and the ITS1-5.8S-ITS2 rDNA region. Trematodes were isolated from so-iuy mullet Liza haematocheila fishes collected in rivers of Primorye and flathead grey mullet Mugil cephalus fishes collected in water bodies of Vietnam (27 fishes in total). A phylogenetic analysis showed that S. spasskii is close to species of the genus Capitimitta of the subfamily Waretrematinae. By intraspecific variation of rDNA sequences, trematodes were divided into three groups with tree different genotypes, which had fixed nucleotide substitutions. Genotype I was found in trematodes from fishes collected in Primorye. Genotype II was detected in trematodes from M. cephalus fishes collected in the Tonkin Bay, Cat Ba Island, Vietnam. Genotype III was found in five trematodes from L. haematocheila collected in the Kievka River, Primorye. The genetic distances between genotypes I and III from Primorye were 0.4 and 0.65% by 28S and ITS rDNA sequences, respectively. The lowest genetic distances were observed between genotypes II (Vietnam) and III (Primorye), 0.1 and 0.33% by 28S and ITS rDNA sequences, respectively. Possible causes of genetic differentiation of S. spasskii from different geographic locations and different definitive host species are discussed.
authors.affiliation: Institute of Biology and Soil Science, Far East Division, Russian Academy of Sciences, Vladivostok, 690022 Russia.|pan2006_82@mail.ru.
authors.forename: D M
authors.initials: DM
authors.lastname: Atopkin
authors.affiliation: Institute of Biology and Soil Science, Far East Division, Russian Academy of Sciences, Vladivostok, 690022 Russia.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Nikitenko
authors.affiliation: Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam.
authors.forename: H D
authors.initials: HD
authors.lastname: Ngo
authors.affiliation: Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam.
authors.forename: N V
authors.initials: NV
authors.lastname: Ha
authors.affiliation: Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam.
authors.forename: N V
authors.initials: NV
authors.lastname: Tang
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes; D058507:Host Specificity; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012800:Siberia; D023701:Smegmamorpha / Q000469:parasitology; D013045:Species Specificity; D014200:Trematoda / Q000145:classification / Q000235:genetics*; D014744:Vietnam
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S
keywords: 28S rDNA; 5.8S rDNA; Haploporidae; ITS rDNA; Mugilidae; PCR; trematodes