title: Metabolic interactions between hyperhomocysteinemia and endothelin-1 among Tunisian patients with acute coronary diseases.
abstract: BACKGROUND
  Acute coronary syndromes (ACS) are complex and polygenic diseases which are a real problem of public health. These syndromes require multidisciplinary studies to understand the pathogenesis mechanisms and metabolic interactions between different risk factors.This study aimed to explore the variation of two coronary risk parameters not mentioned by Framingham cohorts, hyperhomocysteinemia and endothelin-1 (ET-1) in Tunisian coronary and the study of the variation of these parameters based on various cardiac risk factors and metabolic relationship between them.To 157 coronary and 142 healthy subjects, the concentration of homocysteine was quantified by fluorescence polarization immunoassay; the concentration of ET-1 was measured by an analytical technique, the High Performance Liquid Chromatography (HPLC) coupled with mass spectrometry.
  
  
  RESULTS
  Our study showed that homocysteine and ET-1 were significantly higher in patients compared to healthy subjects (24.40 ± 12.5 μmol/L vs 7.44 ± 2.5 μmol/L p <0.00001) for homocysteine and (15.2 ± 5.3 nmol/L vs 7.1 ± 2.7 nmol/L, p <0.00001) for ET-1. On the other hand, homocysteine varies according to tobacco and diabetes while ET-1 depends on the sex, hypertension, smoking, obesity and dyslipidemia and a statistically negative correlation was shown between homocysteine and ET-1 in coronary patients (r = -0.66 p <0.00001).
  
  
  CONCLUSIONS
  The study of the variation of these two parameters in coronary patients and metabolic exploration of the relationship between homocysteine and ET-1 according to various risk factors and the interactions between themselves facilitates the decision of therapeutic treatment.
authors.affiliation: Laboratory of Biochemistry, Farhat HACHED Hospital, Street Doctor Moreau, 4000, Sousse, Tunisia. abdelkader.chalghoum@yahoo.fr.|Valorization and Research Support Space, Biotechnology Center of Borj Cedria, 2052, Hamam Lif, Tunisia. abdelkader.chalghoum@yahoo.fr.
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Chalghoum
authors.affiliation: Laboratory of Biochemistry, Farhat HACHED Hospital, Street Doctor Moreau, 4000, Sousse, Tunisia.
authors.forename: Yosri
authors.initials: Y
authors.lastname: Noichri
authors.affiliation: Valorization and Research Support Space, Biotechnology Center of Borj Cedria, 2052, Hamam Lif, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Karkouch
authors.affiliation: Laboratory of Biochemistry, Farhat HACHED Hospital, Street Doctor Moreau, 4000, Sousse, Tunisia.
authors.forename: Azza
authors.initials: A
authors.lastname: Dandana
authors.affiliation: Department of Biochemistry, Saint-Antoine Hospital, 184 Faubourg Saint-Antoine, 75571, Paris, Cedex 12, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Baudin
authors.affiliation: Department of Cardiology, Farhat HACHED Hospital, Street Doctor Moreau, 4000, Sousse, Tunisia.
authors.forename: Guieder
authors.initials: G
authors.lastname: Jeridi
authors.affiliation: Laboratory of Biochemistry, Farhat HACHED Hospital, Street Doctor Moreau, 4000, Sousse, Tunisia.
authors.forename: Salima
authors.initials: S
authors.lastname: Ferchichi
authors.affiliation: Laboratory of Biochemistry, Farhat HACHED Hospital, Street Doctor Moreau, 4000, Sousse, Tunisia.
authors.forename: Abdelhédi
authors.initials: A
authors.lastname: Miled
mesh_terms: D054058:Acute Coronary Syndrome / Q000378:metabolism*; D000368:Aged; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D020138:Hyperhomocysteinemia / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011446:Prospective Studies; D012307:Risk Factors; D012737:Sex Factors; D013223:Statistics as Topic; D014416:Tunisia
chemical_list: D019332:Endothelin-1; D006710:Homocysteine