title: Supporting Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells on CD34+ Cell Proliferation and Its Mechanism.
abstract: OBJECTIVE
  To investigate the ability of UCB-derived MSCs to support the expansion of HSCs ex vivo and the possible mechanisms involved in this process.
  
  
  METHODS
  HSCs from UCB were co-cultured with UCB-derived MSCs for 14 days, and then the total number of HSCs and colony-forming units (CFU) were detected. Cytokines levels of MSCs supernatant were analyzed using ELISA.
  
  
  RESULTS
  The proliferation rate of HSCs co-cultured with MSCs was significantly higher than that of cultured HSCs alone (P<0.05). Furthermore, the addition of exogenous cytokines to the culture system significantly increased the proliferation rate of HSCs (P<0.05). MSCs had secretion of many cytokines, including GM-CSF, IL-7, IL-8, IL-11, SCF and SDF-1α.
  
  
  CONCLUSIONS
  UCB-derived MSCs as a feeder layer can be an alternative approach for ex vivo expansion of HSCs, and the cytokines by secreted UCB-MSCs may mediate the supportive role of MSC to HSC proliferation.
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors.forename: Zhi-Juan
authors.initials: ZJ
authors.lastname: Xu
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Sheng
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Lou
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors.forename: Qi-Tian
authors.initials: QT
authors.lastname: Mu
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Zhang
authors.affiliation: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China. E-mail: ouyangguifang@medmail.com.cn.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Ouyang
mesh_terms: D018952:Antigens, CD34; D049109:Cell Proliferation; D018920:Coculture Techniques; D016207:Cytokines; D005312:Fetal Blood; D006801:Humans; D059630:Mesenchymal Stem Cells
chemical_list: D018952:Antigens, CD34; D016207:Cytokines