title: Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1.
abstract: The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp. bulgaricus strain OLL1073R-1 was purified and characterized. The molecular mass was 5.0×10(6) g/mol. Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5. The NPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation. Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n. The structure is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains.
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, Saint-Hyacinthe, Québec J2S 8E3, Canada. Electronic address: Marie-Rose.VanCalsteren@AGR.GC.CA.
authors.forename: Marie-Rose
authors.initials: MR
authors.lastname: Van Calsteren
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, Saint-Hyacinthe, Québec J2S 8E3, Canada.
authors.forename: Fleur
authors.initials: F
authors.lastname: Gagnon
authors.affiliation: Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aobaku, Sendai 981-8555, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Nishimura
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Makino
mesh_terms: D002240:Carbohydrate Sequence; D048668:Lactobacillus delbrueckii / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Mass spectrometry (MS); Nuclear magnetic resonance (NMR); Repeating unit sequence; Smith degradation; casper