title: Spag6 Negatively Regulates Neuronal Migration During Mouse Brain Development.
abstract: Sperm-associated antigen 6 (Spag6) is a Chlamydomonas reinhardtii PF16 homologous gene detected in the human testis and is crucial for sperm motility. Neuronal migration is a dynamic process requiring coordinated cytoskeletal remodeling, and Spag6 is co-localized with microtubules in Chinese hamster ovary cells and COS-1 cells. However, the role of Spag6 in neuronal migration remains unclear. Here, we demonstrated that Spag6 was continuously expressed in the developing cerebral cortex. Using in utero electroporation (IUE), we found that overexpression of Spag6 delayed the rate of neuronal migration, rather than affecting the ultimate fate of cortical neurons. Furthermore, overexpression of Spag6 caused a significant decrease in neurite number and length of cortical neurons. Our results indicated that Spag6 controlled neuronal migration as well as neurite branching and elongation.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Runchuan
authors.initials: R
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Mengdi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China. shantingzhao@hotmail.com.
authors.forename: Shanting
authors.identifier: http://orcid.org/0000-0002-5130-7315
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002465:Cell Movement; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D051379:Mice; D008868:Microtubule Proteins / Q000235:genetics / Q000378:metabolism*; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism* / Q000502:physiology
chemical_list: D008868:Microtubule Proteins; C504274:SPAG6 protein, mouse
keywords: In utero electroporation; Neuronal migration; Spag6