title: Antitumor and antimetastasis effects of macerating solutions from an injectable chitosan-based hydrogel on hepatocarcinoma.
abstract: In our previous studies, injectable chitosan-based hydrogel (CH) was prepared and its application in surgery removal of tumor was studied. In this study, the antitumor and antimetastasis effects of the macerating solutions from CH were investigated. Our in vitro results showed that macerating solutions from CH significantly increased the proliferation of human normal liver L02 cells. In contrast, macerating solutions from CH showed significant inhibitory effects on the growth of human hepatoma Bel-7402 cells. In a mouse H22 tumor model, intraperitoneal injection of macerating solutions from CH decreased tumor growth and prevented tumor diffusion. Tumor weight was decreased dramatically in mice treated with macerating solutions from CH. The thymus index and spleen index were significantly increased by treatment with macerating solutions from CH. Administration of macerating solutions from CH also remarkably increased serum levels of TNF-α, IL-2, IFN-γ, and decreased serum VEGF content as compared with the control group treated with saline. The antimetastasis studies showed that the number of pulmonary nodules, pulmonary metastases index, and lymph nodes index were significantly decreased in experimental groups treated with macerating solutions from CH. This study provided more supporting data for the potential clinical application of CH after surgical removal of tumor.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Fulai
authors.initials: F
authors.lastname: Song
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao, 266003, People's Republic of China.
authors.forename: Baoqin
authors.initials: B
authors.lastname: Han
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D007267:Injections; D007376:Interleukin-2 / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008113:Liver Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan
keywords: antimetastasis; antitumor; chitosan-based hydrogel; hepatocarcinoma; tumor surgical removal