title: Identification of an Endophytic Antifungal Bacterial Strain Isolated from the Rubber Tree and Its Application in the Biological Control of Banana Fusarium Wilt.
abstract: Banana Fusarium wilt (also known as Panama disease) is one of the most disastrous plant diseases. Effective control methods are still under exploring. The endophytic bacterial strain ITBB B5-1 was isolated from the rubber tree, and identified as Serratia marcescens by morphological, biochemical, and phylogenetic analyses. This strain exhibited a high potential for biological control against the banana Fusarium disease. Visual agar plate assay showed that ITBB B5-1 restricted the mycelial growth of the pathogenic fungus Fusarium oxysporum f. sp. cubense race 4 (FOC4). Microscopic observation revealed that the cell wall of the FOC4 mycelium close to the co-cultured bacterium was partially decomposed, and the conidial formation was prohibited. The inhibition ratio of the culture fluid of ITBB B5-1 against the pathogenic fungus was 95.4% as estimated by tip culture assay. Chitinase and glucanase activity was detected in the culture fluid, and the highest activity was obtained at Day 2 and Day 3 of incubation for chitinase and glucanase, respectively. The filtrated cell-free culture fluid degraded the cell wall of FOC4 mycelium. These results indicated that chitinase and glucanase were involved in the antifungal mechanism of ITBB B5-1. The potted banana plants that were inoculated with ITBB B5-1 before infection with FOC4 showed 78.7% reduction in the disease severity index in the green house experiments. In the field trials, ITBB B5-1 showed a control effect of approximately 70.0% against the disease. Therefore, the endophytic bacterial strain ITBB B5-1 could be applied in the biological control of banana Fusarium wilt.
authors.affiliation: MOA Key Laboratory of Biology and Genetic Resources for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan Province, 571101, China.
authors.forename: Deguan
authors.initials: D
authors.lastname: Tan
authors.affiliation: MOA Key Laboratory of Biology and Genetic Resources for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan Province, 571101, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Fu
authors.affiliation: MOA Key Laboratory of Biology and Genetic Resources for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan Province, 571101, China.
authors.forename: Bingyin
authors.initials: B
authors.lastname: Han
authors.affiliation: MOA Key Laboratory of Biology and Genetic Resources for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan Province, 571101, China.
authors.forename: Xuepiao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Lijiang Teachers College, Lijiang, Yunnan Province, 674110, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zheng
authors.affiliation: MOA Key Laboratory of Biology and Genetic Resources for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan Province, 571101, China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000935:Antifungal Agents; D061046:Biological Control Agents; D060026:Endophytes / Q000302:isolation & purification*; D060585:Fusariosis / Q000382:microbiology*; D005670:Fusarium; D028482:Hevea / Q000382:microbiology*; D028521:Musa / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents