title: The association of APOC4 polymorphisms with premature coronary artery disease in a Chinese Han population.
abstract: BACKGROUND
  Hypercholesterolemia arising from abnormal lipid metabolism is one of the critical risk factors for coronary artery disease (CAD), however the roles of genetic variants in lipid metabolism-related genes on premature CAD (≤ 60 years old) development still require further investigation. We herein genotyped four single nucleotide polymorphisms (SNPs) in lipid metabolism-related genes (rs1132899 and rs5167 in APOC4, rs1801693 and rs7765781 in LPA), aimed to shed light on the influence of these SNPs on individual susceptibility to early-onset CAD.
  
  
  METHODS
  Genotyping of the four SNPs (rs1132899, rs5167, rs1801693 and rs7765781) was performed in 224 premature CAD cases and 297 control subjects (≤ 60 years old) using polymerase chain reaction-ligation detection reaction (PCR-LDR) method. The association of these SNPs with premature CAD was performed with SPSS software.
  
  
  RESULTS
  Multivariate logistic regression analysis showed that C allele (OR = 1.50, P = 0.027) and CC genotype (OR = 2.84, P = 0.022) of APOC4 rs1132899 were associated with increased premature CAD risk, while the other three SNPs had no significant effect. Further stratified analysis uncovered a more evident association with the risk of premature CAD among male subjects (C allele, OR = 1.65, and CC genotype, OR = 3.33).
  
  
  CONCLUSIONS
  Our data provides the first evidence that APOC4 rs1132899 polymorphism was associated with an increased risk of premature CAD in Chinese subjects, and the association was more significant among male subjects.
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Shun
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Nan-hong
authors.initials: NH
authors.lastname: Li
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Yu-ning
authors.initials: YN
authors.lastname: Chen
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Meng-yun
authors.initials: MY
authors.lastname: Cai
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Sai-sai
authors.initials: SS
authors.lastname: Tang
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Haijiao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Disease, The First People's Hospital of Foshan, Foshan, People's Republic of China.
authors.forename: Jin-ming
authors.initials: JM
authors.lastname: Cen
authors.affiliation: Department of Cardiovascular Disease, The First People's Hospital of Foshan, Foshan, People's Republic of China.
authors.forename: Xi-li
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Department of Cardiovascular Disease, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, People's Republic of China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China. xgliu64@126.com.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China. xgliu64@126.com.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China. xgliu64@126.com.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Dongguan, 523808, People's Republic of China. xiongxingdong@126.com.|Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, People's Republic of China. xiongxingdong@126.com.|Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Zhanjiang, People's Republic of China. xiongxingdong@126.com.
authors.forename: Xing-dong
authors.initials: XD
authors.lastname: Xiong
mesh_terms: D000704:Analysis of Variance; D001056:Apolipoproteins C / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008055:Lipids / Q000097:blood; D017270:Lipoprotein(a) / Q000235:genetics; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: C097349:APOC4 protein, human; D001056:Apolipoproteins C; D008055:Lipids; D017270:Lipoprotein(a)