title: Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway.
abstract: We developed two types of artificial platforms, T-junction and crossroad microchannel devices, and obtained guidance response ratio of pollen tubes to the female tissue as 56-57%. The crossroad device was also able to collect the attracted pollen tubes with high purity, which is useful for future omics analysis.
authors.affiliation: Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan. Electronic address: sato.yoshikatsu@i.mbox.nagoya-u.ac.jp.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
authors.forename: Nagisa
authors.initials: N
authors.lastname: Sugimoto
authors.affiliation: Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan; JST, ERATO, Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan; Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higashiyama
authors.affiliation: JST, ERATO, Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan; Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arata
mesh_terms: D004129:Dimethylpolysiloxanes; D019684:Magnoliopsida / Q000502:physiology*; D055617:Micro-Electrical-Mechanical Systems; D046210:Microfluidic Analytical Techniques; D056771:Ovule / Q000502:physiology*; D053205:Pollen Tube / Q000502:physiology*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon
keywords: Micro-electro-mechanical system; Microchannel; Microchip; Ovule; Plant reproduction; Pollen tube; Pollen tube guidance; Polydimethylsiloxane; Purification; Torenia fournieri