title: A Forward Genetic Screen for Suppressors of Somatic P Granules in Caenorhabditis elegans.
abstract: In Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs of tumor suppressors Retinoblastoma (Rb/LIN-35) and Malignant Brain Tumor (MBT/LIN-61). However, the full scope of pathways that suppress germline expression in the soma is unknown. To address this, we performed a mutagenesis and screened for somatic expression of GFP-tagged PGL-1, a core P-granule nucleating protein. Eight alleles were isolated from 4000 haploid genomes. Five of these alleles exhibit a synMuv phenotype, whereas the remaining three were identified as hypomorphic alleles of known synMuv B genes, lin-13 and dpl-1. These findings suggest that most suppressors of germline programs in the soma of C. elegans are either required for viability or function through synMuv B chromatin regulation.
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672.
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Kelly
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Senter-Zapata
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672.
authors.forename: Anne C
authors.initials: AC
authors.lastname: Campbell
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672.
authors.forename: Hannah E
authors.initials: HE
authors.lastname: Lust
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672.
authors.forename: Monique E
authors.initials: ME
authors.lastname: Theriault
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672.
authors.forename: Karolina M
authors.initials: KM
authors.lastname: Andralojc
authors.affiliation: Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672 dupdike@mdibl.org.
authors.forename: Dustin L
authors.initials: DL
authors.lastname: Updike
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping; D015870:Gene Expression; D017930:Genes, Reporter; D005854:Germ Cells; D009154:Mutation; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; C114543:PGL-1 protein, C elegans; D016601:RNA-Binding Proteins; D012097:Repressor Proteins
keywords: C. elegans; P granules; germline; retinoblastoma; synMuv B