title: [Effect of Chlorogenic Acid on Hepatic Stellate Cell Proliferation, Generation and Degradation of Extracelluar Matrix].
abstract: OBJECTIVE
  To investigate the effects of chlorogenic acid (CGA) on hepatic stellate cell proliferation and the expression and secretion of Collagen I, Collagen III, tissue inhibitors of metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-2 (MMP-2).
  
  
  METHODS
  An immortalized rat hepatic stellate cell (HSC) line was cultured in vitro. The cells were divided into 5 groups: control group; platelet-derived growth factor (PDGF) (10 ng/mL), PDGF+CGA (12.5 µg/mL), PDGF+CGA (25 µg/mL), PDGF+CGA (50 µg/mL) and CGA (50 µg/mL) group. After 24 hours treatment, the proliferation of HSC was detected by MTT method. The mRNA expression of Collagen I, Collagen III, TIMP-1 and MMP-2 were detected by RT-PCR. The protein levels of Collagen I, Collagen III, TIMP-1 and MMP-2 in the culture supernatant of HSC were measured by ELISA.
  
  
  RESULTS
  PDGF increased the hepatic stellate cell proliferation, the mRNA expression and the protein levels of Collagen I, Collagen III and TIMP-1. (P < 0.05), which were significantly decreased by CGA (P < 0. 05). However, CGA had no significant influence on the expression of MMP-2.
  
  
  CONCLUSIONS
  The antifibrotic effect of CGA may be related with the inhibition of hepatic stellate cell proliferation and generation of extracelluar matrix and promotion of extracelluar matrix degradation.
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Shi
authors.forename: A-meng
authors.initials: AM
authors.lastname: Shi
authors.forename: Bo-xin
authors.initials: BX
authors.lastname: Shang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Fei
authors.initials: F
authors.lastname: Dai
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002726:Chlorogenic Acid / Q000494:pharmacology*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D010982:Platelet-Derived Growth Factor; D051381:Rats; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D010982:Platelet-Derived Growth Factor; C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D002726:Chlorogenic Acid; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat