title: Culture Condition Optimization and Pilot Scale Production of the M12 Metalloprotease Myroilysin Produced by the Deep-Sea Bacterium Myroides profundi D25.
abstract: The protease myroilysin is the most abundant protease secreted by marine sedimental bacterium Myroides profundi D25. As a novel elastase of the M12 family, myroilysin has high elastin-degrading activity and strong collagen-swelling ability, suggesting its promising biotechnological potential. Because myroilysin cannot be maturely expressed in Escherichia coli, it is important to be able to improve the production of myroilysin in the wild strain D25. We optimized the culture conditions of strain D25 for protease production by using single factor experiments. Under the optimized conditions, the protease activity of strain D25 reached 1137 ± 53.29 U/mL, i.e., 174% of that before optimization (652 ± 23.78 U/mL). We then conducted small scale fermentations of D25 in a 7.5 L fermentor. The protease activity of strain D25 in small scale fermentations reached 1546.4 ± 82.65 U/mL after parameter optimization. Based on the small scale fermentation results, we further conducted pilot scale fermentations of D25 in a 200 L fermentor, in which the protease production of D25 reached approximately 1100 U/mL. These results indicate that we successfully set up the small and pilot scale fermentation processes of strain D25 for myroilysin production, which should be helpful for the industrial production of myroilysin and the development of its biotechnological potential.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. swjd2010@163.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. ranly1020@126.com.
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Ran
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. wumingyoucai@aliyun.com.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.
authors.forename: Xi-Ying
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. qinqilong@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. qinqilong@sdu.edu.cn.
authors.forename: Qi-Long
authors.initials: QL
authors.lastname: Qin
authors.affiliation: Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.
authors.forename: Bai-Cheng
authors.initials: BC
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D001439:Bacteroides / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D008386:Marine Biology; D045726:Metalloproteases / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D010865:Pilot Projects; D013696:Temperature
chemical_list: D003470:Culture Media; D045726:Metalloproteases; D009584:Nitrogen
keywords: elastase; marine sediment; myroilysin; pilot scale fermentation; protease; single factor experiments; small scale fermentation