title: Bioethanol production from sodium hydroxide/hydrogen peroxide-pretreated water hyacinth via simultaneous saccharification and fermentation with a newly isolated thermotolerant Kluyveromyces marxianu strain.
abstract: In this study, bioethanol production from NaOH/H2O2-pretreated water hyacinth was investigated. Pretreatment of water hyacinth with 1.5% (v/v) H2O2 and 3% (w/v) NaOH at 25 °C increased the production of reducing sugars (223.53 mg/g dry) and decreased the cellulose crystallinity (12.18%), compared with 48.67 mg/g dry and 22.80% in the untreated sample, respectively. The newly isolated Kluyveromyces marxianu K213 showed greater ethanol production from glucose (0.43 g/g glucose) at 45 °C than did the control Saccharomyces cerevisiae angel yeast. The maximum ethanol concentration (7.34 g/L) achieved with K. marxianu K213 by simultaneous saccharification and fermentation (SSF) from pretreated water hyacinth at 42 °C was 1.78-fold greater than that produced by angel yeast S. cerevisiae at 30 °C. The present work demonstrates that bioethanol production achieved via SSF of NaOH/H2O2-pretreated water hyacinth with K. marxianu K213 is a promising strategy to utilize water hyacinth biomass.
authors.affiliation: Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Yan
authors.affiliation: Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming 650500, PR China; Changdu Institute of Agriculture Science, Changdu 854000, PR China.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Qiaoping
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Manman
authors.initials: M
authors.lastname: He
authors.affiliation: Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Li
authors.affiliation: Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Chagan
authors.initials: C
authors.lastname: Irbis
mesh_terms: D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D031423:Hyacinthus / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007716:Kluyveromyces / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012972:Sodium Hydroxide / Q000378:metabolism*
chemical_list: D056804:Biofuels; D000431:Ethanol; D012972:Sodium Hydroxide; D002482:Cellulose; D006861:Hydrogen Peroxide; D002480:Cellulase
keywords: Bioethanol; Kluyveromyces marxianu; NaOH/H(2)O(2) pretreatment; Simultaneous saccharification and fermentation; Water hyacinth