title: Role of an arginine-lysine rich motif in maturation and trafficking of CD19.
abstract: Normal expression of CD19 on the surface of B cells requires the presence of the tetraspanin molecule CD81. Previous studies have shown that surface expression of CD19 is highly reduced in CD81-deficient mouse B cells and that it is completely absent in an antibody deficient human patient with a mutation in the CD81 gene. The current study explored the contribution of an arginine-lysine rich motif, present in the membrane-proximal cytoplasmic domain of CD19, for the maturation and trafficking of this molecule. We demonstrate that this motif plays a role in the maturation and recycling of CD19 but in a CD81-independent manner.
authors.affiliation: Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, CA 94305, USA.
authors.forename: Felipe
authors.initials: F
authors.lastname: Vences-Catalán
authors.affiliation: Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, CA 94305, USA.
authors.forename: Chiung-Chi
authors.initials: CC
authors.lastname: Kuo
authors.affiliation: Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, CA 94305, USA. Electronic address: slevy@stanford.edu.
authors.forename: Shoshana
authors.initials: S
authors.lastname: Levy
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D018941:Antigens, CD19 / Q000737:chemistry / Q000378:metabolism*; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D021381:Protein Transport / Q000502:physiology*; D013329:Structure-Activity Relationship; D060225:Tetraspanin 28 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018941:Antigens, CD19; C554798:CD81 protein, human; D060225:Tetraspanin 28; D001120:Arginine; D008239:Lysine
keywords: CD19; CD81; ER; Glycosylation; Protein trafficking