title: In Vivo Analgesic and Antipyretic Activities of N-Butanol and Water Fractions of Ocimum Suave Aqueous Leaves Extract in Mice.
abstract: BACKGROUND
  Ocimum suave willd is one of the plants traditionally used for the treatment of inflammation and related disorders in different parts of Ethiopia. The aim of the current study was to evaluate the analgesic and antipyretic activities of the solvent fractions (n-butanol and water) of O. suave aqueous leaves extract.
  
  
  METHODS
  Acetic acid writhing and tail flick tests were used to evaluate the analgesic activity, and yeast-induced fever in mice was used to evaluate the antipyretic activity of the solvent fractions.
  
  
  RESULTS
  Both solvent fractions exhibited inhibitory effect against acetic acid induced writhing at all tested dose levels in a dose dependent manner. The water fraction inhibited writhing by 47.69% at a dose of 200 mg/kg which was comparable to that by ASA, the standard drug. In the tail flick test, 200 mg/kg dose of both solvent fractions showed significant activity (P<0.05) after 0.5h, 1h and 3hrs of their administration. Both n- butanol and water fractions produced significant reduction in yeast induced fever at all doses employed.
  
  
  CONCLUSIONS
  From these findings, it can be concluded that the n-butanol and water fractions of O. suave aqueous leaves extract have potential analgesic and antipyretic activity in mice.
authors.affiliation: Department of Pharmacology, College of Medical Sciences, Jimma University, Ethiopia.
authors.forename: Shibiru
authors.initials: S
authors.lastname: Tesema
authors.affiliation: Department of Pharmacology, School of Medicine, Addis Ababa University, Ethiopia.
authors.forename: Eyasu
authors.initials: E
authors.lastname: Makonnen
mesh_terms: D020001:1-Butanol; D019342:Acetic Acid; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D058633:Antipyretics / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005002:Ethiopia; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy; D051379:Mice; D031339:Ocimum / Q000737:chemistry*; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D013623:Tail / Q000187:drug effects / Q000503:physiopathology; D014867:Water; D015003:Yeasts
chemical_list: D000700:Analgesics; D058633:Antipyretics; D010936:Plant Extracts; D014867:Water; D020001:1-Butanol; D019342:Acetic Acid
keywords: Analgesic; O. suave; antipyretic; butanol fraction; tail flick; water fraction; writhing