title: [Joint locus of a/b-globin genes in Danio rerio is segregated into structural subdomains active at different stages of development].
abstract: In the domain model of eukaryotic genome organization, the functional unit of the genome, along with the relevant regulatory elements, is considered to be a gene or a gene family. In hot-blooded vertebrate animals, the domains of a- and b-globin genes are positioned at different chromosomes and are organized and regulated in different fashion. In cold-blooded animals, in particular in tropical fish Danio rerio, a- and b globin genes are located in a common gene cluster. However, the joint a/b-globin gene cluster is subdivided into two development stage-specific subdomains, the adult one and the embryonic-larval one. In an attempt to find out whether this functional segregation correlates with structural segregation of the domain we compared the DNase I sensitivity and profiles of histone modifications of adult and embryonic-larval segments of the domain in cultured D. rerio fibroblasts. We have demonstrated that, in these nonerythroid cells, adult and embryonic- larval subdomains possess different DNase I sensitivities and different profiles of H3K27me3, a histone modification introduced by PRC2 complex. These observations suggest that joint a/b globin gene domain of Danio rerio is segregated into two structural subdomain harboring adult and embryonic-larval globin genes.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Dolgushin
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Petrova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Iudinkova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.|Biology Department, Moscow State University, Moscow, 119991 Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Razin
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.|iarovaia@inbox.ru.
authors.forename: O V
authors.initials: OV
authors.lastname: Iarovaia
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D002874:Chromosome Mapping; D002875:Chromosomes / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry; D004625:Embryo, Nonmammalian; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D056426:Genetic Loci; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D005810:Multigene Family; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development; D055542:alpha-Globins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055544:beta-Globins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D002843:Chromatin; D029941:Fish Proteins; D006657:Histones; D055542:alpha-Globins; D055544:beta-Globins; D011495:Histone-Lysine N-Methyltransferase; D003850:Deoxyribonuclease I
keywords: Danio rerio; a/b-globin genes; chromatin; domains and subdomains within eukaryotic genome