title: Hematological effects of repeated graded doses of the methanol extract of Paullinia pinnata (Linn.) leaves in Wistar albino rats.
abstract: BACKGROUND
  Paullinia pinnata is a medicinal plant used for the treatment of various diseases, including anemia in West Africa.
  
  
  OBJECTIVE
  This study was carried out to investigate the effect of increasing doses of the methanolic leaves extract of P. pinnata on hematological parameters in rats.
  
  
  METHODS
  Thirty-six male Wistar albino rats were grouped into six groups of six animals each. Five doses; 50,100, 200, 400 and 800 mg/kg body weight of the P. pinnata extract were administered separately to five groups. The sixth group served as a control and received only the vehicle (70% physiological saline: 30% Tween 80 [v/v]). Administration was done orally daily for 28 days at 24 h interval. On day 29, the animals were made inactive, blood was then collected from the heart and various hematological parameters were evaluated.
  
  
  METHODS
  Analysis of variance was employed.
  
  
  RESULTS
  The packed cell volume and red blood cell count increased significantly (P < 0.05) in the treatment groups except at 200 mg/kg dose. The hemoglobin concentration increased in all the treatment groups. The values for the neutrophils at 50, 100, 200 and 800 mg/kg doses were higher than that of the control. The white blood cell count increased significantly (P < 0.05) at 50 and 400 mg/kg doses compared to the control and exceeded the normal physiological range.
  
  
  CONCLUSIONS
  The maximum tolerable dose is 200 mg/kg body weight of the methanolic leaves extract of P. pinnata and the extract has anti-anemic property with the ability to increase neutrophils count.
authors.affiliation: Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
authors.forename: Oluwatoyin A
authors.initials: OA
authors.lastname: Adeyemo-Salami
authors.affiliation: Animal Physiology Laboratory, Department of Animal Science, University of Ibadan, Ibadan, Oyo State, Nigeria.
authors.forename: Emmanuel O
authors.initials: EO
authors.lastname: Ewuola
keywords: Erythropoiesis; Paullinia pinnata; hematological parameters; neutrophils