title: Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry.
abstract: The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar. jep2026@qatar-med.cornell.edu.|Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10022, USA. jep2026@qatar-med.cornell.edu.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Pasquier
authors.affiliation: UMR_I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims Champagne-Ardenne, BP-1039-Reims Cedex 2, 51687 Reims, France. damien.rioult@univ-reims.fr.
authors.forename: Damien
authors.initials: D
authors.lastname: Rioult
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar. naa2037@qatar-med.cornell.edu.
authors.forename: Nadine
authors.initials: N
authors.lastname: Abu-Kaoud
authors.affiliation: Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar. jep2026@qatar-med.cornell.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Hoarau-Véchot
authors.affiliation: UGSF, UMR CNRS 8576, équipe Régulation des Signaux de Division, Université de Lille, Sciences et Technologies, 59009 Villeneuve d'Ascq, France. matthieu.marin@univ-lille1.fr.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Marin
authors.affiliation: Laboratory of Ecotoxicology UPRES EA 3222, IFRMP 23, University of Le Havre, 76058 Le Havre, France. frank.lefoll@univ-lehavre.fr.
authors.forename: Frank
authors.initials: F
authors.lastname: Le Foll
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D048429:Cell Size; D018118:Chloride Channels / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D061986:MCF-7 Cells
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018118:Chloride Channels
keywords: MCF7; P-glycoprotein; cell volume; flow cytometer; multi-drug resistance