title: A matter of scale: how emerging technologies are redefining our view of chromosome architecture.
abstract: The 3D folding of the genome and its relation to fundamental processes such as gene regulation, replication, and segregation remains one of the most puzzling and exciting questions in genetics. In this review, we describe how the use of new technologies is starting to revolutionize the field of chromosome organization, and to shed light on the mechanisms of transcription, replication, and repair. In particular, we concentrate on recent studies using genome-wide methods, single-molecule technologies, and super-resolution microscopy (SRM). We summarize some of the main concerns when employing these techniques, and discuss potential new and exciting perspectives that illuminate the connection between 3D genomic organization and gene regulation.
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Diego I
authors.initials: DI
authors.lastname: Cattoni
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Valeri
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Le Gall
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France. Electronic address: marcelo.nollmann@cbs.cnrs.fr.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Nollmann
mesh_terms: D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000737:chemistry* / Q000235:genetics; D004247:DNA / Q000737:chemistry; D023281:Genomics / Q000379:methods*; D006801:Humans; D008853:Microscopy; D008954:Models, Biological; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA
keywords: chromatin structure; chromosome conformation capture; gene expression; single molecule microscopy; super-resolution microscopy