title: [Involvement of oxidative stress in embelin-induced cell death in leukemia HL-60 cells].
abstract: OBJECTIVE
  To evaluate the effects of Embelin on HL-60 cells by the impact of oxidative stress on DNA double-strain breaks (DSBs).
  
  
  METHODS
  HL-60 cells were treated with Embelin in different concentration (3, 10, 30, 100, and 300 μg/ml) for 24 h, and inhibitory effects was examined by CCK-8 assay. Reactive oxygen species (ROS) levels were evaluated by flow cytometry using DCFH-DA. Comet assay was used to detect the extent of DSBs.
  
  
  RESULTS
  Embelin inhibited proliferation of HL-60 cells in a dose-dependent manner. At the concentration of 10, 30, 100, and 300 μg/ml, the inhibition rate was (12.74 ± 2.27)%, (23.49 ± 1.96)%, (30.30±1.89)%, and (57.55 ± 3.59)% (P<0.05). Embelin also lead to high level of intracellular ROS and deterioration of DNA damage (P<0.05). When HL-60 cells were pretreated with ROS scavenger N-acetyl-l-cysteine (NAC) for 2 h and then treated with 300 μg/ml Embelin for 24 h, the intracellular ROS level declined and DSBs relieved (P<0.05). Meanwhile, embelin-induced cell viability significantly declined to (32.75 ± 2.70)% (P<0.05).
  
  
  CONCLUSIONS
  Embelin induced the death of HL-60 cells by increasing the generation of intracellular oxidation and the oxidative stress, which drived the damage of DNA double-strand.
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Miao
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
authors.affiliation: Hematological Department of Shengjing Hospital Affiliated to China Medical University, Shenyang　110023, China.
authors.forename: Zhuogang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000111:Acetylcysteine; D017209:Apoptosis; D016227:Benzoquinones; D002470:Cell Survival; D020552:Comet Assay; D004249:DNA Damage; D005452:Fluoresceins; D018922:HL-60 Cells; D006801:Humans; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
chemical_list: D016227:Benzoquinones; D005452:Fluoresceins; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; C010945:embelin; D000111:Acetylcysteine