title: Targeted tumor imaging of anti-CD20-polymeric nanoparticles developed for the diagnosis of B-cell malignancies.
abstract: The expectations of nanoparticle (NP)-based targeted drug delivery systems in cancer, when compared with convectional therapeutic methods, are greater efficacy and reduced drug side effects due to specific cellular-level interactions. However, there are conflicting literature reports on enhanced tumor accumulation of targeted NPs, which is essential for translating their applications as improved drug-delivery systems and contrast agents in cancer imaging. In this study, we characterized biodegradable NPs conjugated with an anti-CD20 antibody for in vivo imaging and drug delivery onto tumor cells. NPs' binding specificity mediated by anti-CD20 antibody was evaluated on MEC1 cells and chronic lymphocytic leukemia patients' cells. The whole-body distribution of untargeted NPs and anti-CD20 NPs were compared by time-domain optical imaging in a localized human/mouse model of B-cell malignancy. These studies provided evidence that NPs' functionalization by an anti-CD20 antibody improves tumor pharmacokinetic profiles in vivo after systemic administration and increases in vivo imaging of tumor mass compared to non-targeted NPs. Together, drug delivery and imaging probe represents a promising theranostics tool for targeting B-cell malignancies.
authors.affiliation: Department of Life Sciences, University of Trieste, Trieste, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Capolla
authors.affiliation: Institute for Maternal and Child Health - IRCCS "Burlo Garofolo", Trieste, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Garrovo
authors.affiliation: Department of Life Sciences, University of Trieste, Trieste, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Zorzet
authors.affiliation: Animal Care Unit, Cluster in Biomedicine (CBM scrl), Trieste, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lorenzon
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Bologna, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Rampazzo
authors.affiliation: LNK Chemsolutions LLC, Lincoln, NE, USA.
authors.forename: Ruben
authors.initials: R
authors.lastname: Spretz
authors.affiliation: Department of Medical, Surgery and Health Sciences, University of Trieste, Trieste, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pozzato
authors.affiliation: Bio-Target, Inc., University of Chicago, Chicago, IL, USA.
authors.forename: Luis
authors.initials: L
authors.lastname: Núñez
authors.affiliation: Department of Human Pathology, University of Palermo, Palermo, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Tripodo
authors.affiliation: Department of Life Sciences, University of Trieste, Trieste, Italy ; Callerio Foundation Onlus, Institutes of Biological Researches, Trieste, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Macor
authors.affiliation: Institute for Maternal and Child Health - IRCCS "Burlo Garofolo", Trieste, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Biffi
mesh_terms: D000818:Animals; D018951:Antigens, CD20 / Q000737:chemistry*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D015448:Leukemia, B-Cell / Q000175:diagnosis*; D051379:Mice; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D018951:Antigens, CD20; D011108:Polymers
keywords: active targeting; optical imaging; tumor accumulation