title: Expression profile of tumor suppressor gene RASSF1 in lacrimal gland carcinoma.
abstract: We examined the expression pattern of the tumor sup-pressor gene RAS association domain family gene 1 (RASSF1) in lacri-mal gland carcinoma and analyzed its relationship with the oncogenesis and progression of tumors. Sixty-two patients (30 males, 32 females, average age = 47 ± 3.5 years) admitted with lacrimal gland carcinoma to the Department of Ophthalmology of our hospital between January 2012 and January 2014 were enrolled in this study. Based on tumor ma-lignancy, patients were classified into a malignant group (N = 25) and benign group (N = 37). Healthy lacrimal gland resections from trauma surgery (N = 35) were recruited as a healthy control group. Expres-sion profiles of RASSF1 in all groups were quantified using reverse transcription-polymerase chain reaction and western blotting. Recur-rence of lacrimal gland carcinoma was surveyed through postopera-tive follow-up. Expression levels of RASSF1 in samples from the ma-lignant and benign groups were significantly lower than those in the healthy group (P < 0.05). Furthermore, the malignant group showed lower RASSF1 expression than the benign group (P < 0.05). Postopera-tive follow-up identified 22 cases of recurrence in the malignant group, with a recurrence rate of 88%, while 15 cases in the benign group had a recurrence rate of 40.5%. A direct relationship exists between RASSF1 expression levels and the malignancy grade of lacrimal gland carci-noma. Patients with lower RASSF1 expression showed a higher recur-rence probability, indicating unfavorable prognosis. Therefore, measur-ing RASSF1 expression can be used as a diagnostic method for lacrimal gland carcinoma.
authors.affiliation: Ophthalmology Department of West China Hospital, Sichuan University, Sichuan, China changhongzeng88@126.com.
authors.forename: C H
authors.initials: CH
authors.lastname: Zeng
authors.affiliation: Ophthalmology Department of West China Hospital, Sichuan University, Sichuan, China.
authors.forename: B
authors.initials: B
authors.lastname: Guo
authors.affiliation: Ophthalmology Department of West China Hospital, Sichuan University, Sichuan, China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: Ophthalmology Department of West China Hospital, Sichuan University, Sichuan, China.
authors.forename: W M
authors.initials: WM
authors.lastname: He
authors.affiliation: Ophthalmology Department of West China Hospital, Sichuan University, Sichuan, China.
authors.forename: Q L
authors.initials: QL
authors.lastname: Luo
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007765:Lacrimal Apparatus / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins