title: In Vitro Protocols for Measuring the Antioxidant Capacity of Algal Extracts.
abstract: In the last decade a large amount of research has been directed at targeting algal resources for biologically active molecules. High-throughput in vitro antioxidant assays are routinely used to screen for biologically active compounds present in algal extracts when the requirement is to identify samples for progression to more detailed biological scrutiny. Whilst a myriad of antioxidant assays have been developed, this present chapter aims to give step-by-step practical guidance on how to carry out some of the most popular and biologically relevant assays at the bench.
authors.affiliation: Department of Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin, 15, Ireland.
authors.forename: Owen
authors.initials: O
authors.lastname: Kenny
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Brunton
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Smyth
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry; D005470:Fluorometry / Q000379:methods; D005609:Free Radicals / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods; D006997:Hypochlorous Acid / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D012624:Seaweed / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D013858:Thiobarbiturates / Q000737:chemistry; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005609:Free Radicals; D010636:Phenols; D010851:Picrates; D013451:Sulfonic Acids; D013858:Thiobarbiturates; D013861:Thiocyanates; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002338:Carotenoids; C103724:ferric thiocyanate; D006997:Hypochlorous Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; C029684:thiobarbituric acid