title: Reductive Dehalogenation of Oligocyclic Phenolic Bromoaromatics by Dehalococcoides mccartyi Strain CBDB1.
abstract: Dehalococcoides mccartyi strains transform many halogenated compounds and are used for bioremediation. Such anaerobic transformations were intensively studied with chlorinated and simply structured compounds such as chlorinated benzenes, ethenes, and ethanes. However, many halogenated oligocyclic aromatic compounds occur in nature as either naturally produced materials or as part of commercial products such as pharmaceuticals, pesticides, or flame retardants. Here, we demonstrate that the D. mccartyi strain CBDB1 reductively debrominated two oligocyclic aromatic phenolic compounds, tetrabromobisphenol A (TBBPA) and bromophenol blue (BPB). The strain CBDB1 completely converted TBBPA to bisphenol A and BPB to phenol red with a stepwise removal of all bromide substituents. Debromination (but no cell growth) was detected in the cultures cultivated with TBBPA. In contrast, strain CBDB1 grew when interacting with BPB, demonstrating that this substrate was used as an electron acceptor for organobromine respiration. High doses of BPB delayed debromination and inhibited growth in the early cultivation phase. A higher toxicity of TBBPA compared with that of BPB might be due to the higher lipophilicity of TBBPA. Mass spectrometric analyses of whole-cell extracts demonstrated that two proteins encoded by the reductive dehalogenase homologous genes CbdbA1092 and CbdbA1503 were specifically induced by the used oligocyclic compounds, whereas others (e.g., CbdbA84 (CbrA)) were downregulated.
authors.affiliation: †Department of Isotope Biogeochemistry and ‡Department of Analytics, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: †Department of Isotope Biogeochemistry and ‡Department of Analytics, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Kublik
authors.affiliation: †Department of Isotope Biogeochemistry and ‡Department of Analytics, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Cindy
authors.initials: C
authors.lastname: Weidauer
authors.affiliation: †Department of Isotope Biogeochemistry and ‡Department of Analytics, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Seiwert
authors.affiliation: †Department of Isotope Biogeochemistry and ‡Department of Analytics, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Lorenz
authors.initials: L
authors.lastname: Adrian
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D001978:Bromphenol Blue / Q000737:chemistry / Q000378:metabolism*; D041862:Chloroflexi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D054879:Halogenation; D006846:Hydrocarbons, Halogenated / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D011075:Polybrominated Biphenyls / Q000737:chemistry / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001426:Bacterial Proteins; D001559:Benzhydryl Compounds; D001978:Bromphenol Blue; D006846:Hydrocarbons, Halogenated; D010636:Phenols; D011075:Polybrominated Biphenyls; C020806:tetrabromobisphenol A; C006780:bisphenol A; D000098826:Bisphenol A Compounds