title: Proteomic analysis revealed the altered kidney protein profile of a Cyld knockout mouse model.
abstract: The aim of this study was to compare the proteomics pattern of the kidneys from Cyld knockout mice with that from normal mouse kidneys and establish a preliminary understanding of the role of Cyld in the kidney. Proteins from the kidneys of knockout Cyld mice and wild-type mice were extracted, isobaric tags for relative and absolute quantitation (iTRAQ) was performed, and the proteomics patterns of the two groups were compared. The genotypes of the mice were verified by polymerase chain reaction. A total of 1748 proteins with a local false discovery rate of ≤5% were identified, among which 1437 proteins were reliably recognized and quantified. The expression of two dysregulated proteins was confirmed by Western blotting. Gene ontology and pathway analyses indicated that the proteins identified were involved in biological processes, cell components, and molecular functions, and participated in different pathways. Some of the proteins identified were relevant to renal function or kidney diseases. The difference between the proteomics profiles of kidneys from Cyld knockout mice and wild-type mice was prominent, which correlates to kidney dysfunction and the development of renal diseases.
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Song
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: F
authors.initials: F
authors.lastname: Wu
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: S-C
authors.initials: SC
authors.lastname: Sun
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: T X
authors.initials: TX
authors.lastname: Cui
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, China.
authors.forename: D Q
authors.initials: DQ
authors.lastname: Tang
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000235:genetics*; D000074749:Deubiquitinating Enzyme CYLD; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D014176:Protein Biosynthesis / Q000235:genetics; D040901:Proteomics
chemical_list: C511601:CYLD protein, mouse; D000074749:Deubiquitinating Enzyme CYLD; D003546:Cysteine Endopeptidases