title: Inhibition of porcine reproductive and respiratory syndrome virus by Cecropin D in vitro.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) continues to cause substantial economic losses to the pig industry worldwide. Although vaccines are commercially available for the control of PRRSV infection, no vaccination regimen has been proved sustained success in terms of generating a protective immune response. Therefore, the development of novel antivirals is urgently needed. Antimicrobial peptides display broad-spectrum antimicrobial activities against bacteria, fungi, and viruses and play an important role in host innate immune response. Here, we tested whether Cecropin D (CD) could inhibit PRRSV infection and replication in vitro. The inhibitory effect of CD occurred during viral attachment and the early period of viral entry into Marc-145 cells. CD also attenuated virus-induced apoptosis during the late phase of PRRSV infection and suppressed virus release in Marc-145 cells, which might contribute to the inhibition of PRRSV infection. Similar inhibitory effects on PRRSV infection were also found with CD treatment in porcine alveolar macrophages, the major target cell type of PRRSV infection in pigs in vivo. These findings suggest that CD has the potential to develop a new therapeutic agent against PRRSV infection.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, North Third Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, PR China. Electronic address: liuxh8@mail.sysu.edu.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, North Third Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, PR China. Electronic address: 419839537@qq.com.
authors.forename: Chunhe
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR China. Electronic address: ymaohuang@scau.edu.cn.
authors.forename: Yumao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, North Third Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, PR China. Electronic address: 461836869@qq.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, North Third Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, PR China. Electronic address: chyaosh@mail.sysu.edu.cn.
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D002522:Chlorocebus aethiops; D019476:Insect Proteins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000502:physiology; D014176:Protein Biosynthesis / Q000187:drug effects; D034421:Sus scrofa; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D053585:Virus Attachment; D053586:Virus Internalization; D057074:Virus Release; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D019476:Insect Proteins; D014764:Viral Proteins; C036149:cecropin D protein, Hyalophora cecropia
keywords: Antiviral activity; Cecropin D; PRRSV