title: iTRAQ-based quantitative proteomics analysis of rice leaves infected by Rice stripe virus reveals several proteins involved in symptom formation.
abstract: BACKGROUND
  Rice plants infected by Rice stripe virus (RSV) usually leads to chlorosis and death of newly emerged leaves. However, the mechanism of RSV-induced these symptoms was not clear.
  
  
  METHODS
  We used an iTRAQ approach for a quantitative proteomics comparison of non-infected and infected rice leaves. RT-qPCR and Northern blot analyses were performed for assessing the transcription of candidate genes.
  
  
  RESULTS
  As a whole, 681 (65.8% downregulated, 34.2% upregulated infected vs. non-infected) differentially accumulated proteins were identified. A bioinformatics analysis indicated that ten of these regulated proteins are involved in chlorophyll biosynthesis and three in cell death processes. Subsequent RT-qPCR results showed that downregulation of magnesium chelatase was due to reduced expression levels of the genes encoding subunits CHLI and CHLD, which resulted in chlorophyll reduction involved in leaf chlorosis. Three aspartic proteases expressed higher in RSV-infected leaves than those in the control leaves, which were also implicated in RSV-induced cell death. Northern blot analyses of CHLI and p0026h03.19 confirmed the RT-qPCR results.
  
  
  CONCLUSIONS
  The magnesium chelatase and aspartic proteases may be associated with RSV-induced leaf chlorosis and cell death, respectively. The findings may yield new insights into mechanisms underlying rice stripe disease symptom formation.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. bwang0721@163.com.
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. hajanojamal@gmail.com.
authors.forename: Jamal-U-Ddin
authors.initials: JU
authors.lastname: Hajano
authors.affiliation: Institute of Plant Protection, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China. renyd@126.com.
authors.forename: Yingdang
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Institute of Plant Protection, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China. chuantaolu@qq.com.
authors.forename: Chuantao
authors.initials: C
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. wangxifeng@caas.cn.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D057055:Aspartic Acid Proteases / Q000032:analysis / Q000235:genetics; D015152:Blotting, Northern; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008190:Lyases / Q000032:analysis / Q000235:genetics; D012275:Oryza / Q000737:chemistry* / Q000821:virology; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000737:chemistry* / Q000821:virology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D029364:Tenuivirus / Q000254:growth & development*
chemical_list: D020543:Proteome; D057055:Aspartic Acid Proteases; D008190:Lyases; C086788:magnesium chelatase