title: Immunohistochemical localization of GABAergic key molecules in the main olfactory bulb of the Korean roe deer, Capreolus pygargus.
abstract: Gamma-amino butyric acid (GABA) negatively regulates the excitatory activity of neurons and is a predominant neurotransmitter in the nervous system. The olfactory bulb, the main center in the olfactory system, is modulated by inhibitory interneurons that use GABA as their main neurotransmitter. The present study aimed to evaluate GABAergic transmission in the main olfactory bulb (MOB) of the Korean roe deer (Capreolus pygargus) by examining the immunohistochemical localization of GABAergic key molecules, including glutamic acid decarboxylase (GAD), vesicular GABA transporter (VGAT), GABA transporters (GATs; GAT-1 and GAT-3), and potassium sodium chloride co-transporter 2 (KCC2). GAD, VGAT, and KCC2 were expressed in the glomerular layer (GL), external plexiform layer (ePL), mitral cell layer (ML), and granule cell layer (GrL). Intense GAT-1 expression was observed in the GL; GAT-1 expression was discernible in the ePL, ML, and GrL. However, intense GAT-3 expression was extensively observed in all layers of the MOB. These results suggest that substantial GABAergic synapses are present in the GL, ePL, ML, and GrL. Furthermore, the released GABA may be removed by GAT-1 and GAT-3 in the GL, and the majority of GABA, which is present in the ePL to GrL, may undergo reuptake by GAT-3. This is the first morphological and descriptive study of GABAergic transmission in the MOB of Korean roe deer.
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 903 0215, Japan.
authors.forename: Jeongtae
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Molecular Anatomy, Faculty of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 903 0215, Japan.
authors.forename: Chitoshi
authors.initials: C
authors.lastname: Takayama
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690 756, Republic of Korea.
authors.forename: Changnam
authors.initials: C
authors.lastname: Park
authors.affiliation: School of Medicine, Jeju National University, Jeju 690 756, Republic of Korea.
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, 300 Yongbong-Dong, Buk-Gu, Gwangju 500 757, Republic of Korea. Electronic address: moonc@chonnam.ac.kr.
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690 756, Republic of Korea. Electronic address: shint@jejunu.ac.kr.
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D003670:Deer / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D009830:Olfactory Bulb / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*; D000096922:K Cl- Cotransporters
chemical_list: D050485:GABA Plasma Membrane Transport Proteins; D027981:Symporters; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; D005680:gamma-Aminobutyric Acid; D000096922:K Cl- Cotransporters
keywords: Gamma-amino butyric acid; Immunohistochemistry; Main olfactory bulb; Roe deer