title: A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
abstract: Cholesterol and its biosynthetic pathway intermediates and derivatives are required for many developmental processes including membrane biogenesis, transmembrane receptor signaling, steroid biogenesis, nuclear receptor activation, and posttranslational modification of hedgehog (Hh) proteins. To perform such multifaceted tasks depends on stringent regulation of expression of cholesterol biosynthetic enzymes (CBEs). We established for a whole organism, for the first time, the 3D expression pattern of all genes required for cholesterol biosynthesis (CBS), starting from acetyl-CoA and ending with cholesterol. This data was produced by high-throughput in situ hybridization on serial sections through the mouse fetus. The textually annotated image data were seamlessly integrated into the METscout and GenePaint public databases. This novel information helps in the understanding of why CBEs are expressed at particular locations within the fetus. For example, strong CBE expression is detected at sites of cell proliferation and also where cell growth increases membrane surface, such as in neurons sprouting axons and forming synapses. The CBE data also sheds light on the spatial relationship of cells and tissue that express sonic Hh (Shh) and produce cholesterol, respectively. We discovered that not all cells expressing Shh are capable of CBS. This finding suggests novel ways by which cholesterylation of Shh is regulated.
authors.affiliation: Departments of Molecular Biology and Genetics Faculty of Science, Ataturk University, 25240 Erzurum, Turkey.
authors.forename: Melda
authors.initials: M
authors.lastname: Şişecioğlu
authors.affiliation: Departments of Molecular Biology and Genetics Faculty of Science, Ataturk University, 25240 Erzurum, Turkey Genes and Behavior Department, Max Planck Institute of Biophysical Chemistry, 37077 Goettingen, Germany.
authors.forename: Harun
authors.initials: H
authors.lastname: Budak
authors.affiliation: Genes and Behavior Department, Max Planck Institute of Biophysical Chemistry, 37077 Goettingen, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Geffers
authors.affiliation: Genes and Behavior Department, Max Planck Institute of Biophysical Chemistry, 37077 Goettingen, Germany Department of Biology, Faculty of Arts and Sciences, Erzincan University, 24100 Erzincan, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Çankaya
authors.affiliation: Chemistry, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey Department of Chemistry, Faculty of Arts and Sciences, Bingol University, 12000 Bingol, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Çiftci
authors.affiliation: Genes and Behavior Department, Max Planck Institute of Biophysical Chemistry, 37077 Goettingen, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Thaller
authors.affiliation: Genes and Behavior Department, Max Planck Institute of Biophysical Chemistry, 37077 Goettingen, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Eichele
authors.affiliation: Chemistry, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey.
authors.forename: Ömer İrfan
authors.initials: Öİ
authors.lastname: Küfrevioğlu
authors.affiliation: Chemistry, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Özdemir
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000201:enzymology* / Q000378:metabolism; D004734:Energy Metabolism; D018507:Gene Expression Regulation, Developmental; D051379:Mice
chemical_list: D002784:Cholesterol
keywords: Smith-Lemli-Opitz syndrome; brain lipids; cholesterol/biosynthesis; eye; gene expression; in situ hybridization; nutrition; signal transduction