title: Impact of high pressure processing on color, bioactive compounds, polyphenol oxidase activity, and microbiological attributes of pumpkin purée.
abstract: Physicochemical parameters, bioactive compounds' content (carotenoids and total phenols), total antioxidant activity, and enzymatic activity of polyphenol oxidase (PPO) were evaluated after high pressure processing (HPP) on a pumpkin purée (cv. 'Butternut'). Three pressure levels (400, 500, and 600 MPa) were combined with three holding times (200, 400, and 600 s). The applied treatments reduced the levels of total aerobic mesophilic (TAM), total psychrophilic and psychrotrophic bacteria (TPP), and molds and yeasts (M&Y). All applied treatments did not affect enzymatic activity of PPO. Pressure level increased CIE L* values, which could enhance the lightness perception of high pressure (HP)-treated purées. No differences were found between the untreated and HP-treated purées regarding total phenols and carotenoids content (lutein, α-carotene, and β-carotene) and total antioxidant activity. HPP did not affect most quality parameters and maintained the levels of bioactive compounds. However, it did not achieve the complete inhibition of PPO, which could reduce the shelf-life of the pumpkin purée.
authors.affiliation: Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto Tecnológico Agroalimentario, Technological Agri-Food Institute (INTAEX), Badajoz, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: González-Cebrino
authors.affiliation: Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto Tecnológico Agroalimentario, Technological Agri-Food Institute (INTAEX), Badajoz, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Durán
authors.affiliation: Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto Tecnológico Agroalimentario, Technological Agri-Food Institute (INTAEX), Badajoz, Spain.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Delgado-Adámez
authors.affiliation: Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto Tecnológico Agroalimentario, Technological Agri-Food Institute (INTAEX), Badajoz, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Contador
authors.affiliation: Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto Tecnológico Agroalimentario, Technological Agri-Food Institute (INTAEX), Badajoz, Spain mariarosario.ramirez@gobex.es.
authors.forename: Rosario Ramírez
authors.initials: RR
authors.lastname: Bernabé
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D002338:Carotenoids / Q000737:chemistry; D004156:Catechol Oxidase / Q000235:genetics / Q000378:metabolism*; D003116:Color; D028464:Cucurbita / Q000737:chemistry* / Q000382:microbiology*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D010636:Phenols; D011312:Pressure; D014801:Vitamin A
chemical_list: D010636:Phenols; D014801:Vitamin A; D002338:Carotenoids; D004156:Catechol Oxidase
keywords: High hydrostatic pressure; carotenoids; polyphenol oxidase activity; pumpkin purée