title: Cyclam glycoconjugates as lectin ligands and protective agents of metal-induced amyloid aggregation.
abstract: Transition metal ion complexes of a number of chelators have been widely investigated due to their biological properties. The sugar conjugation of metal complexes has resulted in improved properties of the systems, such as solubility and lectin recognition. In this paper, we report the synthesis, the characterization of new glucose and galactose conjugates of 1,4,8,11-tetraazacyclotetradecane (cyclam) and their Cu(II) complexes. The glycoconjugates were proved to be protective agents of metal-induced amyloid aggregation. The binding constants of the galactose conjugate KA1=(3.07±0.16)×10(7) and KA2=(2.13±0.04)×10(7)M(-1) with the lectin Ricinus communis agglutinin (RCA120) as a model of galactose-specific human lectin were obtained with surface plasmon resonance.
authors.affiliation: Istituto di Biostrutture e Bioimmagini (IBB), CNR, via P. Gaifami 18, 95125 Catania, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Lanza
authors.affiliation: Dipartimento di Scienze Chimiche, University of Catania Viale A. Doria 6, 95125 Catania, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: D'Agata
authors.affiliation: Dipartimento di Scienze Chimiche, University of Catania Viale A. Doria 6, 95125 Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Iacono
authors.affiliation: Istituto di Biostrutture e Bioimmagini (IBB), CNR, via P. Gaifami 18, 95125 Catania, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Bellia
authors.affiliation: Dipartimento di Scienze Chimiche, University of Catania Viale A. Doria 6, 95125 Catania, Italy; I.N.B.B. Consortium, Viale delle Medaglie D'Oro, 305, 00136 Roma, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spoto
authors.affiliation: Dipartimento di Scienze Chimiche, University of Catania Viale A. Doria 6, 95125 Catania, Italy. Electronic address: gr.vecchio@unict.it.
authors.forename: Graziella
authors.initials: G
authors.lastname: Vecchio
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000737:chemistry*; D008024:Ligands; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D000682:Amyloid; D056831:Coordination Complexes; D006571:Heterocyclic Compounds; D008024:Ligands; D037121:Plant Lectins; C024560:cyclam; D003300:Copper; D005947:Glucose; D005690:Galactose
keywords: Amyloid; Carbohydrates; Copper; Cyclam; Lectins; Surface plasmon resonance