title: Prediction of development and maintenance of high albuminuria during chronic renin-angiotensin suppression by plasma proteomics.
abstract: BACKGROUND
  High albuminuria is a strong predictor of development of cardiovascular events in hypertensive patients. The search for predictors identifying patients at risk of developing high albuminuria or presenting a more rapid progression in this parameter may represent an effective strategy for adequate intervention and better outcome.
  
  
  RESULTS
  Initially we investigated 24 patients presenting with normoalbuminuria, de novo albuminuria and sustained albuminuria. Plasma proteomics disclosed an upregulation of ceruloplasmin (CP), haptoglobin (HP) and alpha 1-acid glycoprotein (ORM1) that in a second step were selected for validation using turbidimetry assay in a cohort of 105 subjects. The validation showed that HP and ORM1 proteins were increased in patients presenting with very high albuminuria and potential irreversible kidney damage. CP and HP correlated positively with albuminuria values in normoalbuminuric patients. Finally, the levels of ORM1 and CP were increased in patients who progressed in their levels of albuminuria.
  
  
  CONCLUSIONS
  Our findings show that these proteins may potentially be useful for predicting the development of high albuminuria and to monitor renal damage.
authors.affiliation: Laboratorio de Fisiopatologia Vascular, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Baldan-Martin
authors.affiliation: Laboratorio de Fisiopatologia Vascular, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cuesta
authors.affiliation: Departamento de Inmunologia, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Alvarez-Llamas
authors.affiliation: Departamento de Inmunologia, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Gonzalez-Calero
authors.affiliation: Unidad de Hipertension, Instituto de Investigación i+12, Hospital Universitario 12 de Octubre, Madrid, Spain; Instituto Pluridisciplinar, Universidad Complutense de Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Ruiz-Hurtado
authors.affiliation: Laboratorio de Fisiopatologia Vascular, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Moreno-Luna
authors.affiliation: Laboratorio de Fisiopatologia Vascular, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Mourino-Alvarez
authors.affiliation: Laboratorio de Fisiopatologia Vascular, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
authors.forename: Tamara
authors.initials: T
authors.lastname: Sastre-Oliva
authors.affiliation: Unidad de Hipertension, Instituto de Investigación i+12, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Julian
authors.initials: J
authors.lastname: Segura
authors.affiliation: Departamento de Cardiologia, Complejo Hospitalario de Toledo, SESCAM, Toledo, Spain.
authors.forename: Luis R
authors.initials: LR
authors.lastname: Padial
authors.affiliation: Departamento de Inmunologia, IIS-Fundacion Jimenez Diaz, Madrid, Spain; Departamento de Bioquimica y Biologia Molecular I, Universidad Complutense, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.affiliation: Unidad de Hipertension, Instituto de Investigación i+12, Hospital Universitario 12 de Octubre, Madrid, Spain. Electronic address: ruilope@ad-hocbox.com.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Ruilope
authors.affiliation: Laboratorio de Fisiopatologia Vascular, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Barderas
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000419:Albuminuria / Q000097:blood / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood*; D002570:Ceruloplasmin / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000150:complications*; D007668:Kidney / Q000293:injuries; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D009961:Orosomucoid / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012084:Renin-Angiotensin System / Q000187:drug effects*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D006242:Haptoglobins; D009961:Orosomucoid; D002570:Ceruloplasmin
keywords: Albuminuria; Hypertension; Organ damage; Plasma proteomics; RAS suppression