title: EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells.
abstract: OBJECTIVE
  To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells.
  
  
  METHODS
  Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evaluated indirectly by measuring cell viability. Expression of mineralization-associated genes (COL1A1, ALP, BSP, DSPP, RUNX2) in cells cultured on pre-treated dentine for 7 days was determined by RT-qPCR. Nonparametric statistical analysis was performed for cell migration and cell viability data to compare different groups and time-points (Mann-Whitney U-test, α = 0.05).
  
  
  RESULTS
  Treatment of dentine with H2 O or EDTA allowed for cell attachment, which was prohibited by NaOCl with statistical significance (P = 0.000). Furthermore, EDTA conditioning induced cell migration towards dentine. The expression of mineralization-associated genes was increased in dental pulp cells cultured on dentine after EDTA conditioning compared to H2 O-pre-treated dentine discs.
  
  
  CONCLUSIONS
  EDTA conditioning of dentine promoted the adhesion, migration and differentiation of dental pulp stem cells towards or onto dentine. A pre-treatment with EDTA as the final step of an irrigation protocol for regenerative endodontic procedures has the potential to act favourably on new tissue formation within the root canal.
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.
authors.forename: K M
authors.initials: KM
authors.lastname: Galler
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Widbiller
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Buchalla
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Eidt
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.
authors.forename: K-A
authors.initials: KA
authors.lastname: Hiller
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.
authors.forename: P C
authors.initials: PC
authors.lastname: Hoffer
authors.affiliation: Department of Conservative Dentistry and Periodontology, University Medical Center, Regensburg, Germany.|School of Dental Medicine, ZMK Bern, University of Bern, Bern, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Schmalz
mesh_terms: D002113:Calcification, Physiologic / Q000187:drug effects / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology; D003804:Dentin / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology
chemical_list: D004492:Edetic Acid
keywords: EDTA; dental pulp stem cells; dentine; growth factors; regenerative endodontics