title: Histochemical analysis of testis specific gene 13 in human normal and malignant tissues.
abstract: Testis-specific gene 13 (TSGA13) is abundantly expressed in testis. As previous studies of TSGA13 expression pattern have all been based on mRNA analysis, it is imperative to investigate its actual protein expression. Here, we first examined TSGA13 gene tree and protein homology among species, and found that TSGA13 is relatively well conserved. Next, we detected its protein expression in normal human tissues as well as in a limited number of malignant tumors by immunohistochemistry (IHC). It was demonstrated that, in addition to testis, high expression of TSGA13 could also be observed in multiple normal tissues, including stomach, larynx, spleen, bladder, tonsil, liver and thyroid. Notably, most types of human carcinoma tissues displayed reduced expression of TSGA13 rather than their adjacent normal tissues except glioblastoma and lung cancer. Hence, the data from the current study strongly suggest the association between TSGA13 and tumor malignancy.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Stomatology, Air Force General Hospital, Beijing, 100142, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Bu
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Stomatology, Air Force General Hospital, Beijing, 100142, China.
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.affiliation: Department of Oral and Maxillofacial Surgery, Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing, China.
authors.forename: Jinke
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Human Anatomy, Histology, and Embryology, The Fourth Military Medical University, Xi'an, 710032, China. liweipepeyato@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 169 Changle Western Road, Xi'an, 710032, China. sujin923@fmmu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Su
mesh_terms: D000918:Antibody Specificity / Q000276:immunology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D009928:Organ Specificity; D010802:Phylogeny; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D017386:Sequence Homology, Amino Acid; D013736:Testicular Neoplasms / Q000235:genetics / Q000473:pathology; D013737:Testis
chemical_list: D009363:Neoplasm Proteins; D011506:Proteins; C000610983:TSGA13 protein, human
keywords: Immunohistochemistry; TSGA13; Testis-specific; Tissue array; Tumor