title: Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells.
abstract: The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethyl acetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 human lung adenocarcinoma epithelial cells. A-549 cells were exposed to 10-1000 μg/ml concentrations of the leaf extracts of A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced the viability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate, 36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% with water soluble extracts at 1000 μg/ml each. Among the various plant extracts, ethyl acetate extract showed the highest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol, and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of different soluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of a potential therapeutic anticancer agents.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University. Riyadh, Kingdom of Saudi Arabia E-mail : ataweel@hotmail.com.
authors.forename: Areej Mohammad
authors.initials: AM
authors.lastname: Al-Taweel
authors.forename: Shagufta
authors.initials: S
authors.lastname: Perveen
authors.forename: Ghada Ahmed
authors.initials: GA
authors.lastname: Fawzy
authors.forename: Taghreed Abdou
authors.initials: TA
authors.lastname: Ibrahim
authors.forename: Afsar
authors.initials: A
authors.lastname: Khan
authors.forename: Rashad
authors.initials: R
authors.lastname: Mehmood
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects*; D027922:Caprifoliaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D010936:Plant Extracts; D012997:Solvents