title: Bioinspired synthesis of polydopamine/Ag nanocomposite particles with antibacterial activities.
abstract: Mussel-inspired chemistry (polydopamine) offers great opportunities to develop inexpensive and efficient process for many types of materials with complex shapes and functions in a mild and friendly environment. This paper describes a facile, yet green approach to synthesize polydopamine/silver (PDA/Ag) nanocomposite particles with a combination use of polydopamine chemistry and electroless metallization of Ag. In this approach, monodisperse spherical polydopamine particles are first synthesized by the oxidation and self-polymerization of dopamine (monomer) in an alkaline water-ethanol solution at room temperature, which are served as the active templates for secondary reactions due to the abundant catechol and amine groups on the surface. Subsequently, the silver precursor-[Ag(NH3)2](+) ions introduced are easily absorbed onto the surface of the PDA particles, and are immediately in situ reduced to metallic Ag nanoparticles with the help of these active catechol and amine groups. During the preparation, no additional reductants, toxic reagents and intricate instruments are needed. These as-synthesized PDA/Ag nanocomposite particles are ideal candidates for antibacterial application because they do not show significant cytotoxicity against HEK293T human embryonic kidney cells in the in vitro cytotoxicity assay, whereas demonstrate enhanced antibacterial abilities against Escherichia coli (Gram-negative bacteria) and Staphylococcus aureus (Gram-positive bacteria) in the antibacterial assays. Owing to their excellent cytocompatibilities and antibacterial activities, these PDA/Ag nanocomposite particles can be considered as the promising antibacterial materials for future biomedical applications.
authors.affiliation: School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Chengjiao
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.affiliation: School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Zhenni
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address: zwdeng@snnu.edu.cn.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: hlgdz@163.com.
authors.forename: Dingzong
authors.initials: D
authors.lastname: Guo
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom. Electronic address: pengbo006@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D007477:Ions / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D007211:Indoles; D007477:Ions; D011108:Polymers; C568283:polydopamine; D012834:Silver
keywords: Antibacterial activity; Nanocomposite; Particles; Polydopamine; Silver