title: Hyaline cartilage formation and tumorigenesis of implanted tissues derived from human induced pluripotent stem cells.
abstract: Induced pluripotent stem cells (iPSCs) are a promising cell source for cartilage regenerative medicine. Meanwhile, the risk of tumorigenesis should be considered in the clinical application of human iPSCs (hiPSCs). Here, we report in vitro chondrogenic differentiation of hiPSCs and maturation of the differentiated hiPSCs through transplantation into mouse knee joints. Three hiPSC clones showed efficient chondrogenic differentiation using an established protocol for human embryonic stem cells. The differentiated hiPSCs formed hyaline cartilage tissues at 8 weeks after transplantation into the articular cartilage of NOD/SCID mouse knee joints. Although tumors were not observed during the 8 weeks after transplantation, an immature teratoma had developed in one mouse at 16 weeks. In conclusion, hiPSCs are a potent cell source for regeneration of hyaline articular cartilage. However, the risk of tumorigenesis should be managed for clinical application in the future.
authors.affiliation: Bone and Cartilage Regenerative Medicine, Faculty of Medicine, University of Tokyo.
authors.forename: Taku
authors.initials: T
authors.lastname: Saito
authors.forename: Fumiko
authors.initials: F
authors.lastname: Yano
authors.forename: Daisuke
authors.initials: D
authors.lastname: Mori
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawata
authors.forename: Kazuto
authors.initials: K
authors.lastname: Hoshi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.forename: Hideki
authors.initials: H
authors.lastname: Masaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Otsu
authors.forename: Koji
authors.initials: K
authors.lastname: Eto
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Ung-il
authors.initials: UI
authors.lastname: Chung
authors.forename: Sakae
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D020219:Chondrogenesis; D006801:Humans; D051457:Hyaline Cartilage / Q000378:metabolism* / Q000637:transplantation*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID