title: Mam33 promotes cytochrome c oxidase subunit I translation in Saccharomyces cerevisiae mitochondria.
abstract: Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from generating free oxygen radicals. In the yeast Saccharomyces cerevisiae, the nuclear-encoded proteins Mss51 and Pet309 specifically activate mitochondrial translation of the largest subunit, Cox1. Here we report that Mam33 is a third COX1 translational activator in yeast mitochondria. Mam33 is required for cells to adapt efficiently from fermentation to respiration. In the absence of Mam33, Cox1 translation is impaired, and cells poorly adapt to respiratory conditions because they lack basal fermentative levels of Cox1.
authors.affiliation: Department of Molecular Biology, Rowan University School of Osteopathic Medicine, and Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084.
authors.forename: Gabrielle A
authors.initials: GA
authors.lastname: Roloff
authors.affiliation: Department of Molecular Biology, Rowan University School of Osteopathic Medicine, and Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084 henrymf@rowan.edu.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Henry
mesh_terms: D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C112502:MAM33 protein, S cerevisiae; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; C526453:Cox1 protein, S cerevisiae; D003576:Electron Transport Complex IV