title: Assessment of gold nanoparticle effect on prostate cancer LNCaP cells.
abstract: In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and functional effect on tumor cells.
  
  
  OBJECTIVE
  This study was designed to describe the effect of non-functionalized AuNPs on LNCaP prostate cancer cells growth.
  
  
  METHODS
  LNCaP cells were cultured in RPMI-1640 medium containing AuNPs covered by polyvinylpyrrolidone of average size 26.4 nm (10.0 μg/ml). Counts of cells were calculated and their morphology was examined.
  
  
  RESULTS
  AuNPs conglomerates have been visualized in cultured cells. After 4-day incubation in presence of AuNPs significant retardation of LNCaP cells growth was observed both in 5α-dihydrotestosterone stimulated and non-stimulated cultures. No morphological changes of live LNCaP cells were seen in any experiment.
  
  
  CONCLUSIONS
  Given absence of morphological changes in live cells and dribble and relatively constant numbers of dead cells, it was concluded that inhibitory effect of AuNPs on LNCaP cells growth was caused by alterations of proliferation.
authors.affiliation: V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, Kyiv 01601, Ukraine.
authors.forename: A G
authors.initials: AG
authors.lastname: Reznikov
authors.affiliation: V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, Kyiv 01601, Ukraine.
authors.forename: O A
authors.initials: OA
authors.lastname: Salivonyk
authors.affiliation: O.O. Bogomolets Institute of Physiology, Kyiv 01024, Ukraine.
authors.forename: A G
authors.initials: AG
authors.lastname: Sotkis
authors.affiliation: International Center of Molecular Physiology, NAS of Ukraine, Kyiv 01024, Ukraine.
authors.forename: Y M
authors.initials: YM
authors.lastname: Shuba
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape / Q000187:drug effects; D013196:Dihydrotestosterone / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011471:Prostatic Neoplasms / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents; D013196:Dihydrotestosterone; D006046:Gold