title: Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.
abstract: We report the development of a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) instrument (CRS-OCT) capable of measuring analytes in targeted biological tissues with sub-100-micron spatial resolution. The OCT subsystem was used to measure depth-resolved tissue morphology and guide the acquisition of chemically-specific Raman spectra. To demonstrate its utility, the instrument was used to accurately measure depth-resolved, physiologically-relevant concentrations of Tenofovir, a microbicide drug used to prevent the sexual transmission of HIV, in ex vivo tissue samples.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Maher
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Oranat
authors.initials: O
authors.lastname: Chuchuen
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Marcus H
authors.initials: MH
authors.lastname: Henderson
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Rinehart
authors.affiliation: University of North Carolina Eshelman School of Pharmacy and University of North Carolina Center for AIDS Research, University of North Carolina, Chapel Hill, NC, USA ; Department of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA.
authors.forename: Angela D M
authors.initials: AD
authors.lastname: Kashuba
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Wax
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA ; Department of Obstetrics and Gynecology, Duke University, Durham, NC 27708, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Katz
keywords: (170.4500) Optical coherence tomography; (170.5660) Raman spectroscopy; (170.6935) Tissue characterization