title: Environmental factors shaping the abundance and distribution of laccase-encoding bacterial community with potential phenolic oxidase capacity during composting.
abstract: Increasing molecular evidence points to a wide occurrence of laccase-like multicopper oxidase (LMCO)-encoding genes in bacteria. Most researches mainly focused on the bacterial LMCO diversity, whereas the processes and the environmental factors responsible for structuring bacterial LMCO communities remain relatively unknown in a composting system. Six gene libraries were constructed from samples in representative stages during composting. A total of 185 sequences obtained from sample DNA extracts were classified to 59 operational taxonomic units (OTUs) based on 10 % cutoff. The distribution profile of bacterial LMCO genes showed that proteobacterial- and actinobacterial-associated species were the dominant communities during composting. Pearson correlation analysis indicated that the pile temperature and water-soluble carbon (WSC) content were significantly positively correlated with bacterial LMCO gene OTU numbers, Chao1 and Shannon index, whereas the humic acid (HA)-like carbon content had the most significant effect on the distribution of the bacterial LMCO genes during composting by redundancy analysis. These findings will improve the understanding of the mutual relationship between environmental factors and bacterial LMCO community compositions in composting.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China.|Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China.
authors.forename: Lunhui
authors.initials: L
authors.lastname: Lu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China. zgming@hnu.edu.cn.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China. zgming@hnu.edu.cn.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China. fancz@hnu.edu.cn.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China. fancz@hnu.edu.cn.
authors.forename: Changzheng
authors.initials: C
authors.lastname: Fan
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: He
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Ministry of Education, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Changsha, 410082, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
mesh_terms: D058448:Biota; D002244:Carbon / Q000032:analysis; D042845:Laccase / Q000235:genetics*; D014442:Monophenol Monooxygenase / Q000235:genetics*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D012987:Soil; D002244:Carbon; D042845:Laccase; D014442:Monophenol Monooxygenase
keywords: Bacteria; Composting; Environmental factors; Laccase-like multicopper oxidases