title: Diversity and ecological tolerance of bacteria isolated from the rhizosphere of halophyton plants living nearby Kiskunság soda ponds, Hungary.
abstract: Many halophytes and halophilic microorganisms are capable to adapt to the extremities of saline habitats. This study reveals the taxonomic diversity and ecological tolerance of bacteria isolated from the rhizosphere of three different halophytes (Bolboschoenus maritimus, Puccinellia limosa and Aster tripolium) living in the vicinity of Kiskunság soda ponds. Following a sampling in September 2013, altogether 76 bacterial strains were isolated using two different media. The strains were identified on the basis of 16S rRNA gene sequencing following ARDRA grouping. Salt and pH tolerance of the strains were examined by measuring their growth in broths containing 0-15% NaCl (w/V) and characterized with pH 7-12 values. Among the strains genera of Anaerobacillus, Bacillus and Exiguobacterium (Firmicutes), Agromyces, Isoptericola, Microbacterium, Micrococcus, Nocardiopsis, Nesterenkonia and Streptomyces (Actinobacteria), Halomonas and Idiomarina (Proteobacteria) and Anditalea (Bacteroidetes) were identified. The Bolboschoenus and Puccinellia samples characterized with the highest pH and electric conductivity values were dominated by Bacillus, Halomonas and Nesterenkonia, respectively. The salt tolerance of the bacterial strains was strongly dependent on the sampling location and plant species. In contrast, growth of bacterial strains in broths with alkaline pH values was more balanced. The strains from the Puccinellia sample showed the widest salt and pH tolerance.
authors.affiliation: Department of Microbiology, Eötvös Loránd University , Pázmány P. sétány 1/C, H-1117 Budapest , Hungary.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Borsodi
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Bárány
authors.forename: Gergely
authors.initials: G
authors.lastname: Krett
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.forename: Tibor
authors.initials: T
authors.lastname: Szili-Kovács
mesh_terms: D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006814:Hungary; D010802:Phylogeny; D061005:Ponds; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D055051:Salt-Tolerant Plants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: 16S rRNA gene; cultivation; pH and NaCl tolerance; rhizosphere bacteria; soda soil