title: Persistent natural infection of a Culex tritaeniorhynchus cell line with a novel Culex tritaeniorhynchus rhabdovirus strain.
abstract: Culex tritaeniorhynchus rhabdovirus (CTRV) is a mosquito virus that establishes persistent infection without any obvious cell death. Therefore, occult infection by CTRV can be present in mosquito cell lines. In this study, it is shown that NIID-CTR cells, which were derived from Cx. tritaeniorhynchus, are persistently infected with a novel strain of CTRV. Complete genome sequencing of the infecting strain revealed that it is genetically similar but distinct from the previously isolated CTRV strain, excluding the possibility of contamination. These findings raise the importance of further CTRV studies, such as screening of CTRV in other mosquito cell lines.
authors.affiliation: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065.|Institute of Virology, University Medical Center Freiburg, Freiburg 79104, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gillich
authors.affiliation: Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640.
authors.forename: Ryusei
authors.initials: R
authors.lastname: Kuwata
authors.affiliation: Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Isawa
authors.affiliation: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065.|United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8511, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Horie
mesh_terms: D000818:Animals; D002460:Cell Line; D003465:Culex / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: Culex tritaeniorhynchus rhabdovirus; NIID-CTR; occult infection; persistent infection