title: Haplotype analysis of TGF-β1 gene in a preeclamptic population of northern Mexico.
abstract: OBJECTIVE
  To determine the frequencies of -800G/A (rs1800468), -509C/T (rs1800469) and 869T/C (rs1800470) polymorphisms and their haplotypes in the TGF-β1 gene and their association with preeclampsia in a population of northern México.
  
  
  METHODS
  This case-control study involved 175 preeclamptic and 253 normoevolutive pregnant women. The polymorphisms were genotyped by real time PCR.
  
  
  RESULTS
  The allele and genotype frequencies of polymorphisms showed no significant differences between cases and controls; the -800AA genotype had a very low frequency in cases (1%) and controls (0.4%). The TT genotype of the 869T/C polymorphism is a protective factor of severe preeclampsia (OR 0.56, 95% CI 0.32-0.98). The -509C/T and 869T/C polymorphisms were in linkage disequilibrium (D'=.537, p=.009). The most common haplotypes in case and control groups were -800G/-509C/869C, 34.95% and 37.24%, respectively. We found no increased risk of preeclampsia by haplotype.
  
  
  CONCLUSIONS
  Our results suggest that -800G/A, -509C/T and 869T/C polymorphisms of TGF-β1 gene or their haplotypes are not associated with preeclampsia and that only the TT genotype of 869T/C polymorphism is a protective factor of severe preeclampsia in a population of northern México.
authors.affiliation: Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico.
authors.forename: Marisela
authors.initials: M
authors.lastname: Aguilar-Duran
authors.affiliation: Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico.
authors.forename: Jaime
authors.initials: J
authors.lastname: Salvador-Moysén
authors.affiliation: Academia de Genómica, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, IPN Unidad Durango, 34220 Durango, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Galaviz-Hernandez
authors.affiliation: Hospital General de Durango, Secretaría de Salud Durango, 34000 Durango, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vázquez-Alaniz
authors.affiliation: Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico.
authors.forename: Ada A
authors.initials: AA
authors.lastname: Sandoval-Carrillo
authors.affiliation: Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico.
authors.forename: Nadia
authors.initials: N
authors.lastname: Velázquez-Hernández
authors.affiliation: Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, 34000 Durango, Mexico. Electronic address: jsalas_pacheco@hotmail.com.
authors.forename: José M
authors.initials: JM
authors.lastname: Salas-Pacheco
keywords: Haplotype; Polymorphism; Preeclampsia; TGF-β1