title: A visible light induced photoelectrochemical aptsensor constructed by aligned ZnO@CdTe core shell nanocable arrays/carboxylated g-C3N4 for the detection of Proprotein convertase subtilisin/kexin type 6 gene.
abstract: It was reported that Proprotein convertase subtilisin/kexin type 6 (PCSK6) can promote the progression of rheumatoid arthritis to a higher aggressive status. In this work, a novel visible light induced photoelectrochemical (PEC) platform was designed to detect PCSK6 gene. ZnO@CdTe nanocable arrays/carboxylated g-C3N4 used as the PEC signal generator. Hexagonal ZnO nanorods grew on ITO electrode firstly. CdTe were then electrodeposited on the ZnO nanorods surface to enhance the photogenerated h(+)/e(-) separation efficiency. Carboxylated g-C3N4 was utilized to improve h(+)/e(-) separation efficiency and anchor the capture probes of PCSK6 gene by the covalent bonding effect. The 5' and 3' primers captured PCSK6 ssDNA by the specific recognition. The linear range was 10 pg/mL to 20.0 ng/mL with a detection limit of 2 pg/mL.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: Shandong Medicinal Biotechnology Centre, The Key Lab for Biotechnology Drugs of Ministry of Health, The Key Lab of Rare and Uncommon Disease, Jinan 250022, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Pan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Picheng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Youying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: dubin61@gmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D001172:Arthritis, Rheumatoid / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D017931:DNA Primers / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006801:Humans; D008027:Light; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009570:Nitriles / Q000737:chemistry; D043484:Proprotein Convertases / Q000235:genetics*; D012697:Serine Endopeptidases / Q000235:genetics*; D013691:Tellurium / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D017931:DNA Primers; D004277:DNA, Single-Stranded; D009570:Nitriles; C011206:cyanogen; D006108:Graphite; C080654:PCSK6 protein, human; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013691:Tellurium; D015034:Zinc Oxide; C028337:cadmium telluride
keywords: Carboxylated g-C(3)N(4); PCSK6; Photoelectrochemical aptsensor; Rheumatoid arthritis; ZnO@CdTe nanocable arrays