title: Identification and characterization of novel NBS-LRR resistance gene analogues from the pea.
abstract: Pea (Pisum sativum) is one of the most cultivated le-gumes in the world, and its yield and seed quality are affected by a variety of pathogens. In plants, NBS-LRR (nucleotide binding site-leucine-rich repeat) is the main class of disease resistance genes. Using degenerate primers deduced from conserved motifs in the NBS domain of known resistance genes, we identified 10 NBS sequences in three varieties of P. sativum. The deduced amino acid sequences of the iden-tified resistance gene analogues (RGAs) exhibited the typical motifs of the NBS domain (P-loop, kinase-2, kinase-3a, and the hydrophobic domain, GLPL) present in the majority of plant proteins belonging to the NBS-LRR class. Phylogenetic analysis showed that seven RGAs belonged to the non-TIR-NBS-LRR subclass and three to the TIR-NBS-LRR subclass. The results of this study provide insights into the structure of this class of resistance genes in the pea, and their evolution-ary relationships with those of other plant species.
authors.affiliation: Research Unit on Genomics of Crop Insect Pests, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Campus El Manar Tunis, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Djebbi
authors.affiliation: Research Unit on Genomics of Crop Insect Pests, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Campus El Manar Tunis, Tunisia.
authors.forename: D
authors.initials: D
authors.lastname: Bouktila
authors.affiliation: Research Unit on Genomics of Crop Insect Pests, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Campus El Manar Tunis, Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Makni
authors.affiliation: Research Unit on Genomics of Crop Insect Pests, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Campus El Manar Tunis, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Makni
authors.affiliation: Research Unit on Genomics of Crop Insect Pests, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Campus El Manar Tunis, Tunisia mahakm@planet.tn.
authors.forename: M
authors.initials: M
authors.lastname: Mezghani-Khemakhem
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D060467:Disease Resistance / Q000235:genetics* / Q000502:physiology; D005810:Multigene Family; D018532:Pisum sativum / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins