title: [Pandanus tectorius derived caffeoylquinic acids inhibit lipid accumulation in HepG2 hepatoma cells through regulation of gene expression involved in lipid metabolism].
abstract: The fruit of Pandanus tectorius (PTF) has a long history of use as a folk medicine to treat hyperlipidemia in Hainan province, South China. Our previous studies have shown that the n-butanol extract of PTF is rich in caffeoylquinic acids and has an adequate therapeutic effect on dyslipidemic animals induced by high-fat diet. In this work, seven caffeoylquinic acids isolated from PTF were screened for the lipid-lowering activity in HepG2 hepatoma cells. Oil-Red O staining, microscopy and intracellular triglyceride (TG) and total cholesterol (TC) quantification showed that 3-O-caffeoylquinic acid (3-CQA), 3, 5-di-O-caffeoylquinic acid (3,5-CQA), and 3,4,5-tri-O-caffeoylquinic acid (3,4,5-CQA) significantly inhibited lipid accumulation induced by oleic acid and decreased intracellular levels of TC and TG in a dose-dependent manner. These three caffeoylquinic acids showed no significant cytotoxicity at concentrations of 1 -50 μmol x L(-1) as determined by MTT assay. Realtime quantitative PCR revealed that 3-CQA and 3, 5-CQA significantly increased the expression of lipid oxidation-related genes PPARα, CPT-1 and ACOX1 while 3-CQA, 3, 5-CQA and 3,4,5-CQA decreased the expression of lipogenic genes SREBP-1c, SREBP-2, HMGR, ACC, FAS. Overall, 3-CQA, 3, 5-CQA and 3, 4, 5-CQA may be the principal hypolipidemic components in PTF which can decrease intracellular lipid accumulation through up-regulating the expression of lipid oxidative genes and down-regulating the expression of lipogenic genes.
authors.forename: Chong-ming
authors.initials: CM
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Luan
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Xiao-po
authors.initials: XP
authors.lastname: Zhang
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002681:China; D002784:Cholesterol / Q000378:metabolism; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D019301:Oleic Acid; D031673:Pandanaceae / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides / Q000378:metabolism
chemical_list: D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; C472707:caffeoylquinic acid; D011801:Quinic Acid; D019301:Oleic Acid; D002784:Cholesterol