title: Proteome analysis in thyroid pathology.
abstract: The incidence of thyroid cancer has continuously increased due to its detection in the preclinical stage. Clinical research in thyroid pathology is focusing on the development of new diagnostic tools to improve the stratification of nodules that have biological, practical and economic consequences on the management of patients. Several clinical questions related to thyroid carcinoma remain open and the use of proteomic research in the hunt for new targets with potential diagnostic applications has an important role in the solutions. Many different proteomic approaches are used to investigate thyroid lesions, including mass spectrometry profiling and imaging technologies. These approaches have been applied to different human tissues (cytological specimens, frozen sections, formalin-fixed paraffin embedded tissue or Tissue Micro Arrays). Moreover, other specimens are used for biomarker discovery, such as cell lines and the secretome. Alternative approaches, such as metabolomics and lipidomics, are also used and integrated within proteomics.
authors.affiliation: Department of Surgery and Translational Medicine, Section of Pathology, Università degli Studi di Milano-Bicocca, Milan, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Pagni
authors.forename: Vincenzo
authors.initials: V
authors.lastname: L'Imperio
authors.forename: Francesca
authors.initials: F
authors.lastname: Bono
authors.forename: Mattia
authors.initials: M
authors.lastname: Garancini
authors.forename: Gaia
authors.initials: G
authors.lastname: Roversi
authors.forename: Gabriele
authors.initials: G
authors.lastname: De Sio
authors.forename: Manuel
authors.initials: M
authors.lastname: Galli
authors.forename: Andrew James
authors.initials: AJ
authors.lastname: Smith
authors.forename: Clizia
authors.initials: C
authors.lastname: Chinello
authors.forename: Fulvio
authors.initials: F
authors.lastname: Magni
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome; D013959:Thyroid Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D020543:Proteome
keywords: MS-imaging; cancer; diagnosis; mass spectrometry; proteomics; thyroid