title: Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system.
abstract: Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery rates with the short disposable needle vs. the long needle were not significantly different (47.5% and 35.0%, respectively). Twenty-six SCNT embryos were transferred to 13 mares, and one mare delivered a live offspring at Day 342. There was a perfect identity match between the cloned foal and the cell donor after analysis of microsatellite DNA, and the mitochondrial DNA of the cloned foal was identical with that of the oocyte donor. These results demonstrated that the short disposable needle system can be used to recover oocytes to use as cytoplasts for SCNT, in the production of cloned foals and for other applications in equine embryology.
authors.affiliation: Department of Theriogenology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Wonyou
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Theriogenology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Kilyoung
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Surgery, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Inhyung
authors.initials: I
authors.lastname: Lee
authors.affiliation: Department of Life Science, Sogang University, Seoul 04107, Korea.
authors.forename: Hyungdo
authors.initials: H
authors.lastname: Shin
authors.affiliation: Department of Theriogenology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Seongchan
authors.initials: S
authors.lastname: Yeon
authors.affiliation: Department of Theriogenology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.|Emergence Center for Food-Medicine Personalized Therapy System, Advanced Institutes of Convergence Technology, Seoul National University, Suwon 16229, Korea.|Farm Animal Clinical Training and Research Center, Institutes of GreenBio Science Technology, Seoul National University, Pyeongchang 25354, Korea.
authors.forename: Goo
authors.initials: G
authors.lastname: Jang
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000662:veterinary*; D004272:DNA, Mitochondrial / Q000235:genetics; D004624:Embryo Transfer / Q000662:veterinary*; D004622:Embryo, Mammalian; D005260:Female; D006736:Horses; D018895:Microsatellite Repeats; D009339:Needles / Q000662:veterinary; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D054315:Oocyte Retrieval / Q000662:veterinary; D006080:Ovarian Follicle / Q000166:cytology*; D011247:Pregnancy
chemical_list: D004272:DNA, Mitochondrial
keywords: cloned horse; embryo transfer; follicle aspiration; short needle; somatic cell nuclear transfer