title: A Golgi-localized two-photon probe for imaging zinc ions.
abstract: We report a two-photon fluorescent probe which shows a strong two photon excited fluorescence enhancement in response to Zn(2+), easy loading into the cells, Golgi-localizing ability, low cytotoxicity, and high photostability. Two-photon microscopy imaging revealed that this probe allows for real-time monitoring of the changes in Golgi Zn(2+) as well as their 3D distributions in live cells and tissues.
authors.affiliation: Department of Chemistry and Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
authors.forename: Hardev
authors.initials: H
authors.lastname: Singh
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Heo
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Byun
authors.forename: Avik Ranjan
authors.initials: AR
authors.lastname: Sarkar
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006056:Golgi Apparatus / Q000737:chemistry* / Q000648:ultrastructure; D006367:HeLa Cells; D006624:Hippocampus / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton; D061848:Optical Imaging; D051381:Rats; D015032:Zinc / Q000032:analysis*
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D015032:Zinc