title: Paeoniflorin improves menopause depression in ovariectomized rats under chronic unpredictable mild stress.
abstract: Paeoniflorin has been shown to effectively relieve neurologic impairments and lessen depression. It remains poorly understood whether it can be used to treat menopause depression; therefore, in the present study, animal model of menopause depression were established by resecting the ovaries in combined with long-term chronic unpredictable stress. Animal model of menopause depression was established by ovariectomy. Sprague-Dawley rats were given 10 mg/kg of paeoniflorin by gastrogavage for 2 weeks. Fluoxetine (2.5 mg/kg) served as a positive control. Sucrose solution consumption test, open-field test, real-time PCR and western blot results demonstrated that paeoniflorin increased sucrose solution consumption, voluntary behaviors and 5-HT1AR mRNA and protein expression. Paeoniflorin decreased the levels of corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), cortisol (CORT) and 5-HT2AR mRNA and protein expression in rats with menopause depression. These results indicate that paeoniflorin unregulated 5-HT1AR expression, but downregulated 5-HT2AR expression in brains of rats with menopause depression, and thus exert antidepressant effects.
authors.affiliation: Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Shanghai University of Traditional Chinese Medicine Shanghai 200437, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Basic Medical Science of Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Shanghai University of Traditional Chinese Medicine Shanghai 200437, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Shanghai University of Traditional Chinese Medicine Shanghai 200437, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Shanghai University of Traditional Chinese Medicine Shanghai 200437, China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Xia
authors.affiliation: School of Basic Medical Science of Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Lv
authors.affiliation: Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Shanghai University of Traditional Chinese Medicine Shanghai 200437, China.
authors.forename: Li
authors.initials: L
authors.lastname: Dong
keywords: 5-HT1AR; 5-HT2AR; Paeoniflorin; menopause depression; neural regeneration