title: An anion induced multisignaling probe for Hg(2+) and its application for fish kidney and liver tissue imaging studies.
abstract: 3',6'-Bis(diethylamino)-2-(pyridin-2-ylmethyl)spiro[isoindoline-1,9'-xanthen]-3-one () was synthesized for the selective fluorescence and colorimetric recognition of Hg(2+) at pH 6.0. In addition, was useful for imaging Hg(2+) in fish kidney and liver tissues using a fluorescence microscope. Spirolactam ring opening of for Hg(2+) recognition is strongly influenced by the nature of the mercury salt and found to be NO3(-)-induced. Other mercury salts such as HgCl2, Hg(CH3COO)2 and Hg(ClO4)2 failed to induce fluorescence and colorimetric response of under the same experimental conditions. For instance, the former salt does not exhibit spirolactam ring opening but forms a new ionic compound (H3L)2[Hg6Cl18]·2H2O (), whose structure has been elucidated by single crystal X-ray diffraction. This might be explained by (1) the higher covalent nature of Hg(2+) and, hence, the lower acidity of the metal center and its inability to induce the ring opening reaction, and (2) the bulky anion, in the case of Hg(ClO4)2, which is also ionic, faces steric hindrance to accommodate within the N(Et)2 group upon spirolactam ring opening.
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, 713104, West Bengal, India. ddas100in@yahoo.com.
authors.forename: Sandip
authors.initials: S
authors.lastname: Mandal
authors.forename: Arnab
authors.initials: A
authors.lastname: Banerjee
authors.forename: Debasree
authors.initials: D
authors.lastname: Ghosh
authors.forename: Dipak Kumar
authors.initials: DK
authors.lastname: Mandal
authors.forename: Damir A
authors.initials: DA
authors.lastname: Safin
authors.forename: Maria G
authors.initials: MG
authors.lastname: Babashkina
authors.forename: Koen
authors.initials: K
authors.lastname: Robeyns
authors.forename: Mariusz P
authors.initials: MP
authors.lastname: Mitoraj
authors.forename: Piotr
authors.initials: P
authors.lastname: Kubisiak
authors.forename: Yann
authors.initials: Y
authors.lastname: Garcia
authors.forename: Debasis
authors.initials: D
authors.lastname: Das
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D005399:Fishes; D005456:Fluorescent Dyes / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008628:Mercury / Q000032:analysis*; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D061848:Optical Imaging; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013141:Spiro Compounds / Q000737:chemistry*; D044004:Xanthones / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D013141:Spiro Compounds; D044004:Xanthones; C026188:rhodamine 6G; D008628:Mercury