title: Genotoxic and Antigenotoxic Assessment of Chios Mastic Oil by the In Vitro Micronucleus Test on Human Lymphocytes and the In Vivo Wing Somatic Test on Drosophila.
abstract: Chios mastic oil (CMO), the essential oil derived from Pistacia lentiscus (L.) var. chia (Duham), has generated considerable interest because of its antimicrobial, anticancer, antioxidant and other beneficial properties. In the present study, the potential genotoxic activity of CMO as well as its antigenotoxic properties against the mutagenic agent mitomycin-C (MMC) were evaluated by employing the in vitro Cytokinesis Block MicroNucleus (CBMN) assay and the in vivo Somatic Mutation And Recombination Test (SMART). In the in vitro experiments, lymphocytes were treated with 0.01, 0.05 and 0.10% (v/v) of CMO with or without 0.05 μg/ml MMC, while in the in vivo assay Drosophila larvae were fed with 0.05, 0.10, 0.50 and 1.00% (v/v) of CMO with or without 2.50 μg/ml MMC. CMO did not significantly increase the frequency of micronuclei (MN) or total wing spots, indicating lack of mutagenic or recombinogenic activity. However, the in vitro analysis suggested cytotoxic activity of CMO. The simultaneous administration of MMC with CMO did not alter considerably the frequencies of MMC-induced MN and wing spots showing that CMO doesn't exert antigenotoxic or antirecombinogenic action. Therefore, CMO could be considered as a safe product in terms of genotoxic potential. Even though it could not afford any protection against DNA damage, at least under our experimental conditions, its cytotoxic potential could be of interest.
authors.affiliation: Department of Environmental and Natural Resources Management, University of Patras, Agrinio, Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Vlastos
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Drosopoulou
authors.affiliation: Department of Environmental and Natural Resources Management, University of Patras, Agrinio, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Efthimiou
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Maximos
authors.initials: M
authors.lastname: Gavriilidis
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Panagaki
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Krystalenia
authors.initials: K
authors.lastname: Mpatziou
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Kalamara
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Despoina
authors.initials: D
authors.lastname: Mademtzoglou
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Penelope
authors.initials: P
authors.lastname: Mavragani-Tsipidou
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D027927:Pistacia / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry; D020011:Protective Agents; D014921:Wings, Animal / Q000187:drug effects
chemical_list: D010938:Plant Oils; D020011:Protective Agents; D016685:Mitomycin