title: Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma.
abstract: We used a target-centric strategy to identify transporter proteins upregulated in pancreatic ductal adenocarcinoma (PDAC) as potential targets for a functional imaging probe to complement existing anatomical imaging approaches. We performed transcriptomic profiling (microarray and RNASeq) on histologically confirmed primary PDAC tumors and normal pancreas tissue from 33 patients, including five patients whose tumors were not visible on computed tomography. Target expression was confirmed with immunohistochemistry on tissue microarrays from 94 PDAC patients. The best imaging target identified was SLC6A14 (a neutral and basic amino acid transporter). SLC6A14 was overexpressed at the transcriptional level in all patients and expressed at the protein level in 95% of PDAC tumors. Very little is known about the role of SLC6A14 in PDAC and our results demonstrate that this target merits further investigation as a candidate transporter for functional imaging of PDAC.
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Alan R
authors.initials: AR
authors.lastname: Penheiter
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Sibel
authors.initials: S
authors.lastname: Erdogan
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Murphy
authors.affiliation: Biomedical Statistics and Informatics, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Steven N
authors.initials: SN
authors.lastname: Hart
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Joema
authors.initials: J
authors.lastname: Felipe Lima
authors.affiliation: Medical Genome Facility, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Rakhshan Rohakhtar
authors.affiliation: Biomedical Statistics and Informatics, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: O'Brien
authors.affiliation: Biomedical Statistics and Informatics, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Bamlet
authors.affiliation: Department of Epidemiology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Ryan E
authors.initials: RE
authors.lastname: Wuertz
authors.affiliation: Department of Anatomic Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Smyrk
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Fergus J
authors.initials: FJ
authors.lastname: Couch
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: George
authors.initials: G
authors.lastname: Vasmatzis
authors.affiliation: Department of Radiology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Claire E
authors.initials: CE
authors.lastname: Bender
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA ; Department of Radiology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Stephanie K
authors.initials: SK
authors.lastname: Carlson
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral / Q000096:biosynthesis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D014408:Biomarkers, Tumor; C503995:SLC6A14 protein, human