title: Comparison of FcRγ-Deficient and CD57+ Natural Killer Cells Between Cord Blood and Adult Blood in the Cytomegalovirus-Endemic Korean Population.
abstract: BACKGROUND
  FcRγ-deficient natural killer (NK) cells (g(-)NK cells) have been associated with cytomegalovirus (CMV) infection. However, the frequency of g(-)NK cells in a CMV-endemic area (i.e., Korea) has not yet been studied. We examined the frequency of g(-)NK cells and expression of CD57 on NK cells in cord blood (CB) and adult blood (AB).
  
  
  METHODS
  Of the 24 AB samples collected, 95.8% (23/24) were CMV IgG(+)/IgM(-), while 100% of the 13 healthy CB samples were CMV IgG(+)/IgM(-). We performed whole-blood flow cytometry assays to analyze intracellular FcRγ and CD3ζ expression of CD3(-)/CD56(dim) NK cells from 13 CB and 24 AB samples, and surface CD57 expression on CD3(-)/CD56(dim)/CD16(+) NK cells from 13 CB and 19 AB samples.
  
  
  RESULTS
  All CMV seropositive AB samples contained g(-)NK cells (23/23), and the median proportion of g(-)NK cells in the CD3(-)/CD56(dim) NK cell pool was 35.0% (range: 11-77%). CD57(+) NK cells in the CD3(-)/CD56(dim)/CD16(+) NK cell population were detected in all 19 AB samples tested, but not in any CB samples.
  
  
  CONCLUSIONS
  Our data suggest that g(-)NK cells and CD57(+) NK cells are present at a very high frequency in CMV-seropositive AB, but rare in CMV-naïve CB.
authors.affiliation: Department of Pediatrics, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Hee Jo
authors.initials: HJ
authors.lastname: Baek
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. ; Department of Laboratory Medicine, KS Hospital, Gwangju, Korea.
authors.forename: Da-Woon
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. ; Center for Creative Biomedical Scientists, Chonnam National University, Gwangju, Korea. ; Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun, Korea.
authors.forename: Minh-Trang Thi
authors.initials: MT
authors.lastname: Phan
authors.affiliation: Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun, Korea.
authors.forename: Ju-Sun
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Anesthesiology and Pain Medicine, Chonnan National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology and Pain Medicine, Chonnan National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Jeong Il
authors.initials: JI
authors.lastname: Choi
authors.affiliation: Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun, Korea.
authors.forename: Je-Jung
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Myung-Geun
authors.initials: MG
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Ryang
authors.affiliation: Department of Companion & Laboratory Animal Science, Kongju National University, Yesan, Korea.
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Pediatrics, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Kook
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. ; Center for Creative Biomedical Scientists, Chonnam National University, Gwangju, Korea. ; Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun, Korea.
authors.forename: Duck
authors.initials: D
authors.lastname: Cho
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D044466:Asian People; D019002:CD56 Antigen / Q000378:metabolism*; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D017452:Receptors, IgG / Q000172:deficiency / Q000235:genetics*; D056910:Republic of Korea / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D019002:CD56 Antigen; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D017452:Receptors, IgG
keywords: Adult; Blood; Cord; Cytomegalovirus; FcRγ; Infection; Natural killer cell