title: Chemical shift assignments of a new folded domain from yeast Pcf11.
abstract: The yeast protein Pcf11 is a component of the cleavage/polyadenylation factor IA (CF IA) complex involved in the 3' processing of pre-mRNA. Pcf11 interacts with RNA and the C-terminal domain (CTD) of the largest subunit of RNA polymerase II via the CTD-interaction domain (CID), and other peptide regions mediate contacts with CF IA subunits Clp1 and Rna14/Rna15. We have identified a novel domain adjacent to the CID and have determined the backbone and sidechain (1)H, (13)C and (15)N chemical shift assignments for the bacterially produced construct. Despite the reduced sequence complexity due to numerous glutamine and leucine residues, secondary chemical shift analysis indicates that the domain is composed of three well-defined helical regions with relaxation measurements consistent with a folded independent domain. The proximity of this previously uncharacterized domain close to the N-terminal CID prompts speculation for a putative role in modulating CTD and RNA binding, or possible intermolecular contacts within CF IA.
authors.affiliation: Inserm U869, 2 rue Robert Escarpit, 33607, Pessac, France.|Institut Européen de Chimie et Biologie, Univ. Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Xu
authors.affiliation: Inserm U869, 2 rue Robert Escarpit, 33607, Pessac, France.|Institut Européen de Chimie et Biologie, Univ. Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France.
authors.forename: Natacha
authors.initials: N
authors.lastname: Pérébaskine
authors.affiliation: Inserm U869, 2 rue Robert Escarpit, 33607, Pessac, France.|Institut Européen de Chimie et Biologie, Univ. Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Minvielle-Sébastia
authors.affiliation: Inserm U869, 2 rue Robert Escarpit, 33607, Pessac, France.|Institut Européen de Chimie et Biologie, Univ. Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fribourg
authors.affiliation: Inserm U869, 2 rue Robert Escarpit, 33607, Pessac, France. c.mackereth@iecb.u-bordeaux.fr.|Institut Européen de Chimie et Biologie, Univ. Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France. c.mackereth@iecb.u-bordeaux.fr.
authors.forename: Cameron D
authors.initials: CD
authors.lastname: Mackereth
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000737:chemistry*
chemical_list: C512637:PCF11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D039221:mRNA Cleavage and Polyadenylation Factors
keywords: Pcf11; Pre-mRNA; Protein domain; RNA processing; Yeast