title: Comparative analysis of transcription factor gene families from Papaver somniferum: identification of regulatory factors involved in benzylisoquinoline alkaloid biosynthesis.
abstract: Opium poppy (Papaver somniferum L.), known for biosynthesis of several therapeutically important benzylisoquinoline alkaloids (BIAs), has emerged as the premier organism to study plant alkaloid metabolism. The most prominent molecules produced in opium poppy include narcotic analgesic morphine, the cough suppressant codeine, the muscle relaxant papaverine and the anti-microbial agent sanguinarine and berberine. Despite several health benefits, biosynthesis of some of these molecules is very low due to tight temporal and spatial regulation of the genes committed to their biosynthesis. Transcription factors, one of the prime regulators of secondary plant product biosynthesis, might be involved in controlled biosynthesis of BIAs in P. somniferum. In this study, identification of members of different transcription factor gene families using transcriptome datasets of 10 cultivars of P. somniferum with distinct chemoprofile has been carried out. Analysis suggests that most represented transcription factor gene family in all the poppy cultivars is WRKY. Comparative transcriptome analysis revealed differential expression pattern of the members of a set of transcription factor gene families among 10 cultivars. Through analysis, two members of WRKY and one member of C3H gene family were identified as potential candidates which might regulate thebaine and papaverine biosynthesis, respectively, in poppy.
authors.affiliation: CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.
authors.forename: Parul
authors.initials: P
authors.lastname: Agarwal
authors.affiliation: CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.
authors.forename: Sumya
authors.initials: S
authors.lastname: Pathak
authors.affiliation: CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Lakhwani
authors.affiliation: CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.
authors.forename: Parul
authors.initials: P
authors.lastname: Gupta
authors.affiliation: CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.
authors.forename: Mehar Hasan
authors.initials: MH
authors.lastname: Asif
authors.affiliation: CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India. prabodht@hotmail.com.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India. prabodht@hotmail.com.
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
mesh_terms: D044182:Benzylisoquinolines / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005810:Multigene Family; D010207:Papaver / Q000235:genetics* / Q000378:metabolism; D010208:Papaverine / Q000096:biosynthesis / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D064210:Secondary Metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D044182:Benzylisoquinolines; D010940:Plant Proteins; D014157:Transcription Factors; D010208:Papaverine
keywords: Benzylisoquinoline alkaloids; Biosynthetic pathway; Cultivars; Papaver somniferum; Transcription factors; Transcriptome data