title: Effectiveness of inhibitor rapamycin, saracatinib, linsitinib and JNJ-38877605 against human prostate cancer cells.
abstract: To investigate the effect of different concentrations of inhibitors rapamycin, saracatinib, linsitinib and JNJ-38877605 on PC-3 cells with CCK-8 assay, respectively. PC-3 cells were incubated with different concentrations of rapamycin, saracatinib, linsitinib and JNJ-38877605, respectively, for 48 h at 37°C, the concentrations of rapamycin were 5 nM, 10 nM, 20 nM, 50 nM, 75 nM, 100 nM; Saracatinib: 0.125 nM, 0.25 nM, 0.5 nM, 1 nM, 2.5 nM, 5 nM; Linsitinib: 2 nM, 5 nM, 10 nM, 20 nM, 40 nM, 60 nM; JNJ-38877605: 0.125 nM, 0.5 nM, 1 nM, 2.5 nM, 5 nM, 10 nM. The proliferation of PC-3 cells was examined by CCK-8. Different concentrations of inhibitor rapamycin remarkably inhibited PC-3 cell proliferation after 48 h (P<0.05), inhibitory action did not change significantly from 5 nM-100 nM; different concentrations of saracatinib, linsitinib and JNJ-38877605 did not inhibit PC-3 cell proliferation after 48 h. Rapamycin treatment at low concentration can inhibit the proliferation of PC-3 cells, while saracatinib, linsitinib and JNJ-38877605 do not inhibit PC-3 cell proliferation.
authors.affiliation: Department of Urology, Linyi People's Hospital Linyi 276000, China ; Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200011, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Urology, Jinshan Hospital, Fudan University Shanghai 201508, China.
authors.forename: Zhanyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Urology, Linyi People's Hospital Linyi 276000, China.
authors.forename: Longmei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, Linyi People's Hospital Linyi 276000, China.
authors.forename: Xiangfei
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Urology, Linyi People's Hospital Linyi 276000, China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Urology, Linyi People's Hospital Linyi 276000, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Urology, Linyi People's Hospital Linyi 276000, China.
authors.forename: Fengfu
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200011, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Urology, Jinshan Hospital, Fudan University Shanghai 201508, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
keywords: JNJ-38877605; PC-3 cells; linsitinib; rapamycin; saracatinib