title: Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture.
abstract: Centromeres play a pivotal role in maintaining genome integrity by facilitating the recruitment of kinetochore and sister-chromatid cohesion proteins, both required for correct chromosome segregation. Centromeres are epigenetically specified by the presence of the histone H3 variant (CENH3). In this study, we investigate the role of the highly conserved γ-tubulin complex protein 3-interacting proteins (GIPs) in Arabidopsis centromere regulation. We show that GIPs form a complex with CENH3 in cycling cells. GIP depletion in the gip1gip2 knockdown mutant leads to a decreased CENH3 level at centromeres, despite a higher level of Mis18BP1/KNL2 present at both centromeric and ectopic sites. We thus postulate that GIPs are required to ensure CENH3 deposition and/or maintenance at centromeres. In addition, the recruitment at the centromere of other proteins such as the CENP-C kinetochore component and the cohesin subunit SMC3 is impaired in gip1gip2. These defects in centromere architecture result in aneuploidy due to severely altered centromeric cohesion. Altogether, we ascribe a central function to GIPs for the proper recruitment and/or stabilization of centromeric proteins essential in the specification of the centromere identity, as well as for centromeric cohesion in somatic cells.
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France;
authors.forename: Morgane
authors.initials: M
authors.lastname: Batzenschlager
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research OT Gatersleben, D-06466 Stadt Seeland, Germany;
authors.forename: Inna
authors.initials: I
authors.lastname: Lermontova
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research OT Gatersleben, D-06466 Stadt Seeland, Germany;
authors.forename: Veit
authors.initials: V
authors.lastname: Schubert
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research OT Gatersleben, D-06466 Stadt Seeland, Germany;
authors.forename: Jörg
authors.initials: J
authors.lastname: Fuchs
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France;
authors.forename: Alexandre
authors.initials: A
authors.lastname: Berr
authors.affiliation: Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH, Amsterdam, The Netherlands.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Koini
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France;
authors.forename: Guy
authors.initials: G
authors.lastname: Houlné
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France;
authors.forename: Etienne
authors.initials: E
authors.lastname: Herzog
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research OT Gatersleben, D-06466 Stadt Seeland, Germany;
authors.forename: Twan
authors.initials: T
authors.lastname: Rutten
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France;
authors.forename: Abdelmalek
authors.initials: A
authors.lastname: Alioua
authors.affiliation: Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH, Amsterdam, The Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: Fransz
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France;
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Schmit
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67000 Strasbourg, France; marie-edith.chaboute@ibmp-cnrs.unistra.fr.
authors.forename: Marie-Edith
authors.identifier: http://orcid.org/0000-0001-8688-721X
authors.initials: ME
authors.lastname: Chabouté
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle; D002503:Centromere; D017343:Genes, Plant; D006657:Histones / Q000378:metabolism; D011485:Protein Binding
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C509716:GIP1 protein, Arabidopsis; C000602368:GIP2 protein, Arabidopsis; D006657:Histones
keywords: Arabidopsis; MZT1; centromere assembly; centromeric cohesion; ploidy stability