title: Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis.
abstract: Antimicrobial peptides (AMPs) have a great potential to be used as a substitute for antibiotics since AMPs don't lead to bacteria's drug resistance. Anti-lipopolysaccharide factors (ALFs) are one type of AMPs and exist in crustaceans. In the present study, we produced a recombinant protein (rFcALF5) of an ALF isoform (FcALF5) from Chinese shrimp Fenneropenaeus chinensis through a prokaryotic expression system. The rFcALF5 exhibited varied antibacterial activities against different bacteria. Besides its antibacterial activities, it could also inhibit the infection of white spot syndrome virus (WSSV) to shrimp after pre-incubation with this virus. In order to learn the antiviral mechanism on how rFcALF5 influences WSSV infection, the interaction between the total proteins of WSSV and rFcALF5 was analyzed and the data showed that rFcALF5 had direct interaction with the envelope protein VP24 of WSSV. The LPS binding domain (LBD) of FcALF5 also showed direct interaction with VP24 of WSSV. Therefore we inferred that the antiviral activity of FcALF5 might be achieved through the binding of its LBD to VP24 of WSSV. These findings provided more information to develop new strategies for the control of shrimp disease in aquaculture.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao 266071, China.
authors.forename: Shihao
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao 266071, China. Electronic address: fhli@qdio.ac.cn.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xinjia
authors.initials: X
authors.lastname: Lv
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D060829:Arthropod Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008070:Lipopolysaccharides / Q000276:immunology; D008826:Microbial Sensitivity Tests; D033561:Penaeidae; D020033:Protein Isoforms / Q000235:genetics; D017434:Protein Structure, Tertiary; D014759:Viral Envelope Proteins / Q000187:drug effects; D046848:White spot syndrome virus 1 / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents; D060829:Arthropod Proteins; D008070:Lipopolysaccharides; D020033:Protein Isoforms; D014759:Viral Envelope Proteins; C034705:antilipopolysaccharide factor (Limulus)
keywords: Anti-lipopolysaccharide factors; Antibacterial; Antiviral; VP24; WSSV