title: Transcription Analysis of the Beta-Glucosidase Precursor in Wild-Type and l-4i Mutant Bombyx mori (Lepidoptera: Bombycidae).
abstract: Lethal fourth-instar larvae (l-4i) mutant of Bombyx mori, a recently discovered novel mutant, die from energy depletion due to genetic mutation. Beta-glucosidase is a common digestive enzyme that hydrolyzes cellulose in the diet to provide energy. In this study, the mRNA expression profiles of B. mori beta-glucosidase precursor (BmpreBG) were characterized by reverse transcription polymerase chain reaction and quantitative real-time polymerase chain reaction. The transcription level of BmpreBG varied in different tissues and developmental stages, except in the pupa and moth, which are the no-diet period. Remarkably, the mRNA expression level of BmpreBG was sharply reduced in l-4i but not in the wild type, which suggested that the digestive function of the mutant was severely damaged. This was consistent with the l-4i phenotypic traits of not eating mulberries, lack of energy, and ultimate death. 5'-rapid amplification of cDNA ends showed, for the first time, that BmpreBG has a 160-bp 5'-untranslated region. These findings suggested that B. mori β-glucosidase precursor was involved in the death process of l-4i mutant larvae.
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Lequn
authors.initials: L
authors.lastname: Kang
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China The Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China qlzhao302@126.com.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D017344:Genes, Insect; D005804:Genes, Lethal; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D019476:Insect Proteins; D012333:RNA, Messenger; D001617:beta-Glucosidase
keywords: BmpreBG; lethal mutant; β-glucosidase precursor