title: Preparation of Graphene-Modified Acupuncture Needle and Its Application in Detecting Neurotransmitters.
abstract: We report a unique nanosensing platform by combining modern nanotechnology with traditional acupuncture needle to prepare graphene-modified acupuncture needle (G-AN), and using it for sensitive detection of neurotransmitters via electrochemistry. An electrochemical deposition method was employed to deposit Au nanoparticles (AuNPs) on the tip surface of the traditional acupuncture needle, while the other part of the needle was coated with insulation paste. Subsequently, the G-AN was obtained by cyclic voltammetry reduction of a graphene oxide solution on the surface of the AuNPs. To investigate the sensing property of the G-AN, pH dependence was measured by recording the open circuit potential in the various pH buffer solutions ranging from 2.0 to 10.0. What's more, the G-AN was further used for detection of dopamine (DA) with a limit of detection of 0.24 μM. This novel G-AN exhibited a good sensitivity and selectivity, and could realize direct detection of DA in human serum.
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Tang
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Danxin
authors.initials: D
authors.lastname: Du
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: School of Acupuncture and Moxibustion, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Liang
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ning
authors.affiliation: 1] School of Acupuncture and Moxibustion, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China [2] Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1] School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China [2] Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D026881:Acupuncture / Q000295:instrumentation; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004298:Dopamine / Q000032:analysis / Q000097:blood; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009339:Needles; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results
chemical_list: D018377:Neurotransmitter Agents; D006046:Gold; D006108:Graphite; D004298:Dopamine