title: Exploiting Oligo(amido amine) Backbones for the Multivalent Presentation of Coiled-Coil Peptides.
abstract: The investigation of coiled coil formation for one mono- and two divalent peptide-polymer conjugates is presented. Through the assembly of the full conjugates on solid support, monodisperse sequence-defined conjugates are obtained with defined positions and distances between the peptide side chains along the polymeric backbone. A heteromeric peptide design was chosen, where peptide K is attached to the polymer backbone, and coiled-coil formation is only expected through complexation with the complementary peptide E. Indeed, the monovalent peptide K-polymer conjugate displays rapid coiled-coil formation when mixed with the complementary peptide E sequence. The divalent systems show intramolecular homomeric coiled-coil formation on the polymer backbone despite the peptide design. Interestingly, this intramolecular assembly undergoes a conformational rearrangement by the addition of the complementary peptide E leading to the formation of heteromeric coiled coil-polymer aggregates. The polymer backbone acts as a template bringing the covalently bound peptide strands in close proximity to each other, increasing the local concentration and inducing the otherwise nonfavorable formation of intramolecular helical assemblies.
authors.affiliation: †Department of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Ulla I M
authors.initials: UI
authors.lastname: Gerling-Driessen
authors.affiliation: ‡Max Planck Institute of Colloids and Interfaces, Research Campus Golm 14424 Potsdam, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Mujkic-Ninnemann
authors.affiliation: ‡Max Planck Institute of Colloids and Interfaces, Research Campus Golm 14424 Potsdam, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ponader
authors.affiliation: †Department of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Schöne
authors.affiliation: ‡Max Planck Institute of Colloids and Interfaces, Research Campus Golm 14424 Potsdam, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Hartmann
authors.affiliation: †Department of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Koksch
authors.forename: U I M
authors.initials: UI
authors.lastname: Gerling-Driessen
authors.forename: D
authors.initials: D
authors.lastname: Schöne
authors.forename: B
authors.initials: B
authors.lastname: Koksch
authors.forename: D
authors.initials: D
authors.lastname: Ponader
authors.forename: N
authors.initials: N
authors.lastname: Mujkic-Ninnemann
authors.forename: L
authors.initials: L
authors.lastname: Hartmann
mesh_terms: D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D000588:Amines; D009841:Oligonucleotides; D010455:Peptides; D011108:Polymers