title: Comparison of biochemical and immunological profile of pediatric patients with acute myeloid leukemia in relation to healthy individuals.
abstract: OBJECTIVE
  To compare the biochemical and immunological profiles of pediatric patients with acute myeloid leukemia (AML) with healthy children and adolescents.
  
  
  METHODS
  This was a cross-sectional study in which 21 therapy-naïve patients with AML were compared with a group of 24 healthy individuals. The following data were analyzed: serum proteins, leucocytes and subgroups, erythrocytes, hematocrit, hemoglobin, platelets, cytokines in peripheral blood mononuclear cells cultures under spontaneous and BCG- or PHA-stimulated conditions, immunoglobulin A, and erythrocytic glutathione. Statistical analysis was performed using SPSS software, considering as significant p-values<0.05.
  
  
  RESULTS
  Serum albumin levels were higher (p<0.0001) in the control group, as well as all the parameters related to red blood cells (p<0.0001). For leucocytes and subgroups, no statistical difference was found between the AML and the control groups. For cytokines, the concentrations were significantly higher under spontaneous and BCG-stimulated conditions for TNF-α, IL-6, IL-10, and IFN-γ in the control group. Under PHA-stimulated conditions, the concentration was higher (p=0.002) only for IL-6. No difference was found between the two groups for the other cytokines and for IgA in the saliva. Erythrocytic glutathione was higher (p<0.0001) in AML patients.
  
  
  CONCLUSIONS
  It was possible to characterize the biochemical and immunological profile of pediatric patients with AML, as well as highlight some significant differences in these parameters when comparing with healthy children and adolescents.
authors.affiliation: Nutrition Course, Center for Biological and Health Sciences, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil. Electronic address: fabianelaflor@gmail.com.
authors.forename: Fabiane L F Z
authors.initials: FL
authors.lastname: Sanches
authors.affiliation: Centre for Research in Pediatrics, Department of Pediatrics, Faculty of Medical Sciences, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Taís M
authors.initials: TM
authors.lastname: Nitsch
authors.affiliation: Centre for Research in Pediatrics, Department of Pediatrics, Faculty of Medical Sciences, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Maria Marluce S
authors.initials: MM
authors.lastname: Vilela
authors.affiliation: Department of Food and Nutrition, Faculty of Food Engineering, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Valdemiro C
authors.initials: VC
authors.lastname: Sgarbieri
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007223:Infant; D007231:Infant, Newborn; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D011228:Prealbumin / Q000032:analysis; D012463:Saliva / Q000276:immunology; D012709:Serum Albumin / Q000032:analysis; D055815:Young Adult
chemical_list: D016207:Cytokines; D007071:Immunoglobulin A, Secretory; D011228:Prealbumin; D012709:Serum Albumin; D005978:Glutathione
keywords: Avaliação Bioquímica; Avaliação Imunológica; Biochemical assessment; Immune evaluation; Leucemia Mieloide Aguda; Leukemia; Pediatria; Pediatrics