title: [Study of change in activity of hepatic drug metabolism enzymes in rat model of chronic unpredictable mild stress].
abstract: This study aimed to explore the impact of depression caused by chronic unpredictable mild stress (CUMS) on in vivo activity of six kinds of CYP450 isoforms in rats. According to 'Katz' method, the model of CUMS was established. Tolbutamide, chlorzoxazone, theophylline, midazolam, omeprazole and dextromethorphan were chosen as probe substrates of CYP2C6, CYP2E1, CYP1A2, CYP3A2, CYP2D1 and CYP2D2 of rats. Plasma concentration of six kinds of CYP450 in control group and model group were determined by LC-MS/MS and computed pharmacokinetic parameters. Consequently, metabolism of theophylline and chlorzoxazone accelerated significantly (P < 0.01), but tolbutamide, dextromethorphan, omeprazole and midazolam had no significant difference. The present study proved that depression caused by CUMS had strong induction to CYP1A2 and medium induction to CYP2E1.
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Zang
authors.forename: Bing-ting
authors.initials: BT
authors.lastname: Sun
authors.forename: Wen-zhu
authors.initials: WZ
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Rong
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Dai
authors.forename: Wen-zheng
authors.initials: WZ
authors.lastname: Ju
authors.forename: Heng-shan
authors.initials: HS
authors.lastname: Tan
mesh_terms: D000818:Animals; D002753:Chlorzoxazone / Q000378:metabolism; D002853:Chromatography, Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003863:Depression; D003915:Dextromethorphan / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008874:Midazolam / Q000378:metabolism; D009853:Omeprazole / Q000378:metabolism; D051381:Rats; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D013806:Theophylline / Q000378:metabolism; D014044:Tolbutamide / Q000378:metabolism
chemical_list: D003915:Dextromethorphan; D003577:Cytochrome P-450 Enzyme System; D014044:Tolbutamide; D013806:Theophylline; D002753:Chlorzoxazone; D009853:Omeprazole; D008874:Midazolam