title: Novel sulfated phosphoglycolipids from Natronomonas moolapensis.
abstract: Polar lipid pattern determination is often used for the taxonomic classification of halophilic Archaea in addition to a genomic characterization. During the analysis of polar lipid extracts from the recently described haloarchaeon Natrononomonas moolapensis, an unknown glycolipid was detected. Fragmentation patterns observed from preliminary mass spectrometric analysis initially suggested the presence of a sulfo-hexosyl-phosphatidylglycerol. However, by NMR spectroscopy and enzymatic assays the existence of two isomeric molecules with different hexoses (1-(6-sulfo-d-glcp/galf-β1,2-glycero)-phospho-2,3-diphytanylglycerol) could be shown. The structural origin from phosphatidylglycerol distinguishes these glycolipids within Archaea, because all other characterized haloarchaeal glycolipids consist of diphytanylglycerol directly linked to an oligoglycosyl moiety. Now the door is open to investigate the physical and functional consequences of these architectural differences of the head groups.
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoffmann
authors.affiliation: Institute of Physics, Martin Luther University Halle-Wittenberg, Betty-Heimann-Str. 7, 06120 Halle, Germany; Department of Chemistry, Umeå University, 90187 Umeå, Sweden.
authors.forename: Michael
authors.initials: M
authors.lastname: Kovermann
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Oberwinkler
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Siedler
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Goethe University, Max-von-Laue-Str. 15, 60438 Frankfurt, Germany.
authors.forename: Niña Socorro
authors.initials: NS
authors.lastname: Cortina
authors.affiliation: Institute of Physics, Martin Luther University Halle-Wittenberg, Betty-Heimann-Str. 7, 06120 Halle, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Balbach
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Oesterhelt
mesh_terms: D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000032:analysis* / Q000378:metabolism; D006216:Halobacteriaceae / Q000145:classification / Q000378:metabolism*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D010802:Phylogeny; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000737:chemistry
chemical_list: D006017:Glycolipids; D010715:Phosphatidylglycerols; D010743:Phospholipids; D013431:Sulfates
keywords: Halophilic archaea; Mass; NMR; Natronomonas moolapensis strain 8.8.11(T); Phosphatidylglycerol; spectrometry