title: Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi.
abstract: Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.
authors.affiliation: Translation Research Center, Inha University School of Medicine, Incheon, Korea.
authors.forename: Sun-Myoung
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
authors.forename: Hea Yoon
authors.initials: HY
authors.lastname: Kwon
authors.affiliation: Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
authors.forename: Jae-Hyong
authors.initials: JH
authors.lastname: Im
authors.affiliation: Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Baek
authors.affiliation: Department of Microbiology, Inha University School of Medicine, Incheon, Korea.
authors.forename: Jae-Seung
authors.initials: JS
authors.lastname: Kang
authors.affiliation: Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D006801:Humans; D008854:Microscopy, Electron; D012285:Orientia tsutsugamushi / Q000276:immunology* / Q000378:metabolism; D012612:Scrub Typhus / Q000175:diagnosis / Q000382:microbiology; D022142:Secretory Vesicles / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface
keywords: Antigens; Orientia tsutsugamushi; Outer Membrane Vesicles; Scrub Typhus