title: Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription.
abstract: Target miRNA was detected by producing RNA: rolling circle polymerization (RCP) and rolling circle transcription (RCT) were interlinked to provide dual amplification, producing multiplied malachite green (MG) aptamers, and a signal was generated by the SERS (surface-enhanced Raman scattering) quantification of the MG molecules that were bound to the transcripts.
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China. greeneese@live.cn.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.forename: Fuwei
authors.initials: F
authors.lastname: Zheng
authors.forename: Rui
authors.initials: R
authors.lastname: Ren
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D004270:DNA, Circular / Q000737:chemistry* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D058105:Polymerization; D012394:Rosaniline Dyes / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D052157:Aptamers, Nucleotide; D004270:DNA, Circular; D035683:MicroRNAs; D012394:Rosaniline Dyes; C005095:malachite green