title: Quantification of nardosinone in rat plasma using liquid chromatography-tandem mass spectrometry and its pharmacokinetics application.
abstract: A rapid, sensitive and high-throughput liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was established and validated to assay the concentration of nardosinone, a main active compound isolated from Nardostachys chinensis, in rat plasma. Plasma samples were processed by protein precipitation with acetonitrile and separated on a Venusil MP-C18 column (50 × 2.1 mm, 5 µm) at an isocratic flow rate of 0.6 mL/min using methanol-0.1% formic acid in water (55 : 45, v/v) as mobile phase, and total run time was 2.5 min. MS-MS detection was accomplished in selected reaction monitoring mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 9.60-320 ng/mL with lower limit of quantification of 9.60 ng/mL. The intra- and inter-day precisions were below 12.3% in terms of relative standard deviation, and the accuracy was within ±9.0% in terms of relative error. Extraction recovery, matrix effect and stability were also satisfactory in rat plasma. The developed method was successfully applied to a pharmacokinetic study of nardosinone following an intravenous injection at a dose of 1.04 mg/kg to Sprague-Dawley rats.
authors.affiliation: Department of Pharmacy, Linyi People's Hospital, Linyi 276003, PR China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Assets and Equipment, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Pediatric Department of Internal Medicine, Linyi People's Hospital, Linyi 276003, PR China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Skill Training Room, Linyi People's Hospital, Linyi 276003, PR China jingrong243714@hotmail.com.
authors.forename: Jingrong
authors.initials: J
authors.lastname: Su
authors.affiliation: Yantai Yuhuangding Hospital, Yantai 264000, PR China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Yi
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D057230:Limit of Detection; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D000081223:Polycyclic Sesquiterpenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C011513:nardosinone