title: Time-Lapse Imaging of Glial-Axonal Interactions.
abstract: In the central nervous system (CNS), myelin is formed by oligodendrocytes that are derived from precursor cells, known as oligodendrocyte precursor cells (OPCs). Successive stages of OPC interactions with the axons can be visualized in vitro and ex vivo using mixed neural cell cultures and pieces of intact spinal cord, respectively. OPCs and their differentiation can be imaged using cell-type-specific markers or green fluorescent protein (GFP) tags. This protocol describes methodology for generating these two systems for time-lapse imaging of dynamic cell interactions using fluorescent and 2-photon microscopy.
authors.affiliation: Clinical Tumor Biology & Immunotherapy Group, Ludwig Centre for Cancer Research, Department of Oncology, University Hospital of Lausanne, Lausanne, Switzerland.|These authors contributed equally to this work.
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Ioannidou
authors.affiliation: Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom.|These authors contributed equally to this work.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Edgar
authors.affiliation: Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Barnett
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D002490:Central Nervous System / Q000166:cytology*; D005456:Fluorescent Dyes / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000382:microbiology; D059008:Time-Lapse Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes
keywords: CNS; myelination; time-lapse imaging