title: Generation and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease.
abstract: Advances in differentiation of cardiomyocytes from human induced pluripotent stem cell (hiPSC) were emerged as a tool for modeling of cardiovascular disease that recapitulates the phenotype for the purpose of drug screening, biomarker discovery, and testing of single-nucleotide polymorphism (SNP) as a modifier for disease stratification. Here, we describe the (1) retroviral reprogramming strategies in the generation of human iPSC, (2) methodology in characterization of iPSC in order to identify the stem cell clones with the best quality, and (3) protocol of cardiac differentiation by modulation of Wnt signaling and β-catenin pathway.
authors.affiliation: Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: Yee Ki
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: X
authors.initials: X
authors.lastname: Ran
authors.affiliation: Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: K W H
authors.initials: KW
authors.lastname: Lai
authors.affiliation: Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: V Y M
authors.initials: VY
authors.lastname: Lau
authors.affiliation: Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.|Research Center of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.|Shenzhen Institutes of Research and Innovation, University of Hong Kong, Hong Kong, China.|Division of Cardiology, Queen Mary Hospital, Hong Kong, China.
authors.forename: D C W
authors.initials: DC
authors.lastname: Siu
authors.affiliation: Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. hftse@hku.hk.|Research Center of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. hftse@hku.hk.|Shenzhen Institutes of Research and Innovation, University of Hong Kong, Hong Kong, China. hftse@hku.hk.|Hong Kong-Guangdong Joint Laboratory on Stem Cell and Regenerative Medicine, Hong Kong, China. hftse@hku.hk.|Division of Cardiology, Queen Mary Hospital, Hong Kong, China. hftse@hku.hk.
authors.forename: H F
authors.initials: HF
authors.lastname: Tse
mesh_terms: D000577:Amides / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D004622:Embryo, Mammalian; D058732:Embryoid Bodies / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D061252:Feeder Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D061251:Primary Cell Culture; D011509:Proteoglycans / Q000737:chemistry; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012190:Retroviridae / Q000235:genetics; D013724:Teratoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D000577:Amides; D015415:Biomarkers; C495270:CTNNB1 protein, human; C473711:Chir 99021; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D007797:Laminin; D011509:Proteoglycans; D011725:Pyridines; D011743:Pyrimidines; D051176:beta Catenin; C059485:matrigel; C108830:Y 27632; D003094:Collagen
keywords: Cardiac differentiation; Cardiovascular disease modeling; Human induced pluripotent stem cell (hiPSC); Retroviral reprogramming; Stem cell characterization