title: Genetically encoded voltage indicators for large scale cortical imaging come of age.
abstract: Electrical signals are fundamental to cellular sensing, communication and motility. In the nervous system, information is represented as receptor, synaptic and action potentials. Understanding how brain functions emerge from these electrical signals is one of the ultimate challenges in neuroscience and requires a methodology to monitor membrane voltage transients from large numbers of cells at high spatio-temporal resolution. Optical voltage imaging holds longstanding promises to achieve this, and has gained a fresh powerful momentum with the development of genetically encoded voltage indicators (GEVIs). With a focus on neuroimaging studies on intact mouse brains, we highlight recent advances in this field.
authors.affiliation: Department of Medicine, Imperial College London, London W12 0NN, United Kingdom. Electronic address: tknopfel@knopfel-lab.net.
authors.forename: Thomas
authors.initials: T
authors.lastname: Knöpfel
authors.affiliation: Department of Medicine, Imperial College London, London W12 0NN, United Kingdom.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Gallero-Salas
authors.affiliation: Department of Medicine, Imperial College London, London W12 0NN, United Kingdom.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Song
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000378:metabolism / Q000502:physiology; D002460:Cell Line; D005071:Evoked Potentials / Q000502:physiology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D059907:Functional Neuroimaging / Q000379:methods*; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D015202:Protein Engineering; D056969:Voltage-Sensitive Dye Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins