title: Short Communication: Effect of heat stress on heat-shock protein (Hsp60) mRNA expression in rainbow trout Oncorhynchus mykiss.
abstract: The enhanced expression of heat shock proteins (hsps) in organisms can be detected in response to many kinds of stressor. For fish, high temperature is an important stressor, and hsp expression is associated with differences in environmental temperature. In this study, rainbow trout (Oncorhynchus mykiss) that were accustomed to an aquatic temperature of 18°C were exposed to an elevated temperature (25°C), and hsp60 expression in the gill, liver, spleen, heart, and head kidney was quantified using real-time polymerase chain reaction in unstressed and heat-stressed animals. The fish responded to heat stress in a time- and tissue-specific manner. Cardiac hsp60 mRNA levels were largely unchanged, and the greatest induction of hsp60 in heat-stressed animals was recorded in the liver, suggesting that protein damage and the consequent requirement for the Hsp60 protein are probably greater in hepatic tissue. Therefore, fish must be provided with optimal temperature conditions in order to realize their potential growth and maximize fish farm profits.
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
authors.forename: H N
authors.initials: HN
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China liuz@gsau.edu.cn.
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Kang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
authors.forename: W M
authors.initials: WM
authors.lastname: Wang
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D005880:Gills / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D018834:Chaperonin 60; D012333:RNA, Messenger