title: Decreased Expression of MiRNA-204-5p Contributes to Glioma Progression and Promotes Glioma Cell Growth, Migration and Invasion.
abstract: Gliomas are the most common malignant primary brain tumors in adults and exhibit a spectrum of aberrantly aggressive phenotype. Although increasing evidence indicated that the deregulation of microRNAs (miRNAs) contributes to tumorigenesis and invasion, little is known about the roles of miR-204-5p in human gliomas. In the present study, the expression of miR-204-5p in clinical glioma tissues was measured by qRT-PCR. The effects of miR-204-5p on glioma cell growth and metastasis were examined by overexpressing or inhibiting miR-204-5p. We found that the expression level of miR-204-5p was significantly reduced in clinical glioma tissues compared with normal brain tissues. Moreover, we revealed that the introduction of miR-204-5p dramatically suppressed glioma cell growth, migration and invasion. Furthermore, mechanistic investigations revealed that RAB22A, a member of the RAS oncogene family, is a direct functional target of miR-204-5p in gliomas. In vivo, restoring miR-204-5p expression in glioma cells suppressed tumorigenesis and increased overall host survival. Our findings suggest that miR-204-5p is a cancer suppressor miRNA and overexpression of miR-204-5p is a novel glioma treatment strategy.
authors.affiliation: Department of Microbiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100730, China; School of Basic Medicine, Peking Union Medical College, Beijing 100730, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Suzhou Wuzhong Hospital, Suzhou 210031, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Suzhou Wuzhong Hospital, Suzhou 210031, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100730, China; School of Basic Medicine, Peking Union Medical College, Beijing 100730, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D005910:Glioma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics; D011379:Prognosis; D012334:RNA, Neoplasm / Q000235:genetics / Q000502:physiology*; D014410:Tumor Stem Cell Assay; D023041:Xenograft Model Antitumor Assays; D020691:rab GTP-Binding Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C549404:MIRN204 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; C484191:RAB22A protein, human; D012334:RNA, Neoplasm; D020691:rab GTP-Binding Proteins