title: Characterization of Phlorotannins from Brown Algae by LC-HRMS.
abstract: Phlorotannins are a class of polyphenols found in brown seaweeds that have significant potential for use as therapeutics, owing to their wide range of bioactivities. Molecular characterization of phlorotannin-enriched extracts is challenging due to the extreme sample complexity and the wide range of molecular weights observed. Herein, we describe a method for characterizing phlorotannins employing ultrahigh-pressure liquid chromatography (UHPLC) operating in hydrophilic interaction liquid chromatography (HILIC) mode combined with high-resolution mass spectrometry (HRMS).
authors.affiliation: Measurement Science and Standards, National Research Council of Canada, 1200 Montreal Road, Ottawa, ON, Canada, K1A 0R6, jeremy.melanson@nrc-cnrc.gc.ca.
authors.forename: Jeremy E
authors.initials: JE
authors.lastname: Melanson
authors.forename: Shawna L
authors.initials: SL
authors.lastname: MacKinnon
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D000459:Phaeophyceae / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis / Q000302:isolation & purification; D012624:Seaweed / Q000737:chemistry*; D013634:Tannins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D059808:Polyphenols; D013634:Tannins