title: Duplex Ratio Tests as Diagnostic Biomarkers in Malignant Melanoma.
abstract: Chromosomal instability is a well-described feature of malignant tumors. Melanomas have typical patterns of chromosomal instability compared with benign nevi, which have minimal DNA copy number change. A few malignant melanomas and their benign counterparts, nevi, prove difficult to diagnose on histopathologic analysis alone, which is currently the gold standard. Quantitative PCR-based assays called duplex ratio tests (DRTs) have been developed by our laboratory for application using DNA from FFPE samples of melanomas and nevi. The reproducibility and accuracy of the DRTs were demonstrated and appropriate correction factors for DNA quality calculated for each assay, based on the results of 108 diploid samples. As a panel, seven DRTs were able to differentiate unambiguous cases of melanoma and nevi with a sensitivity of 87% (95% CI, 83%-91%) and a specificity of 88% (95% CI, 84%-92%) in a series of 145 melanomas and 123 nevi. The DRT scores for 20 nonmetastasizing primary melanomas and 20 metastasizing primary melanomas revealed that DRTs had a marginal benefit as prognostic markers. DRTs have early potential to act as molecular biomarkers of melanoma on FFPE specimens pending validation, and DRTs may have applicability as prognostic markers in melanoma or other tumor types if new DRTs to relevant loci are developed.
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom. Electronic address: dam18@le.ac.uk.
authors.forename: David A
authors.initials: DA
authors.lastname: Moore
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom.
authors.forename: Gerald
authors.initials: G
authors.lastname: Saldanha
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom.
authors.forename: Abdlrzag
authors.initials: A
authors.lastname: Ehdode
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom.
authors.forename: Mohamed Z
authors.initials: MZ
authors.lastname: Mughal
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom.
authors.forename: Linda
authors.initials: L
authors.lastname: Potter
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom.
authors.forename: Lovesh
authors.initials: L
authors.lastname: Dyall
authors.affiliation: Department of Cancer Studies, University of Leicester, Leicester, United Kingdom.
authors.forename: James H
authors.initials: JH
authors.lastname: Pringle
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D056915:DNA Copy Number Variations; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000235:genetics; D008875:Middle Aged; D009506:Nevus / Q000175:diagnosis / Q000235:genetics; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000235:genetics; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor