title: Expression profile of IL-1 family cytokines in aqueous humor and sera of patients with HLA-B27 associated anterior uveitis and idiopathic anterior uveitis.
abstract: The purpose of this study was to evaluate the cytokine expression profile of specific IL-1 family members in the aqueous humor and sera of patients with HLA-B27 associated acute anterior uveitis (AAU) and idiopathic AAU. Following informed consent, a total of 13 patients with HLA-B27 associated AAU, 12 patients with idiopathic AAU and 9 controls were recruited to this study from May 2013 to July 2014. Each individual received a complete ophthalmologic examination. Aqueous humor and sera samples were collected and 11 inflammation-related cytokines of the IL-1 family (IL-1α, IL-1β, IL-1 receptor antagonist [IL-1Ra], IL-18, IL-36 receptor antagonist [IL-36Ra], IL-33, IL-36α, IL-36β, IL-36γ, IL-37, IL-38) were quantitatively measured and analyzed for statistical significance between groups. The degree of inflammation, anterior chamber cell or flare, correlated with expression of IL-1β, IL-1Ra, and IL-18. The highest levels of IL-1β, IL-1Ra, IL-18, and IL-36Ra were seen in the aqueous of patients with HLA-B27 associated AAU and this was statically significant when compared to the controls, but not to idiopathic AAU. Expression of IL-18 was statistically higher in the aqueous of patients with HLA-B27 associated AAU in comparison to either idiopathic AAU or controls, but this may reflect greater inflammation in this patient group. In the sera only IL-1α was statistically higher in the HLA-B27 associated AAU in comparison to the control. Cytokine analysis reveals elevation of multiple IL-1 family members in the aqueous humor of patients with AAU as compared to controls. The specific signature of inflammation may potentially be useful in developing new future therapies for AAU.
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Jules Stein Eye Institute, David Geffen School of Medicine at the University of California, Los Angeles, USA.
authors.forename: Lynn
authors.initials: L
authors.lastname: Gordon
authors.affiliation: Eye & ENT Hospital, Department of Ophthalmology & Vision Science, Shanghai Medical School, Fudan University, Shanghai, China; Department of Ophthalmology, University of Hong Kong, Hong Kong, China. Electronic address: linglingchen98@hotmail.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D002452:Cell Count; D005260:Female; D015796:HLA-B27 Antigen / Q000378:metabolism*; D006650:Histocompatibility Testing; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014606:Uveitis, Anterior / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015796:HLA-B27 Antigen; D007375:Interleukin-1
keywords: Acute anterior uveitis; Aqueous humor; Cytokines; HLA-B27; IL-1 family; IL-37; Idiopathic AAU; Serum