title: Development of a cost-efficient novel method for rapid, concurrent genotyping of five common single nucleotide polymorphisms of the brain derived neurotrophic factor (BDNF) gene by tetra-primer amplification refractory mutation system.
abstract: Brain derived neurotrophic factor (BDNF) is a molecular trophic factor that plays a key role in neuronal survival and plasticity. Single nucleotide polymorphisms (SNPs) of the BDNF gene have been associated with specific phenotypic traits in a large number of neuropsychiatric disorders and the response to psychotherapeutic medications in patient populations. Nevertheless, due to study differences and occasionally contrasting findings, substantial further research is required to understand in better detail the association between specific BDNF SNPs and these psychiatric disorders. While considerable progress has been made recently in developing advanced genotyping platforms of SNPs, many high-throughput probe- or array-based detection methods currently available are limited by high costs, slow processing times or access to advanced instrumentation. The polymerase chain reaction (PCR)-based, tetra-primer amplification refractory mutation system (T-ARMS) method is a potential alternative technique for detecting SNP genotypes efficiently, quickly, easily, and cheaply. As a tool in psychopathology research, T-ARMS was shown to be capable of detecting five common SNPs in the BDNF gene (rs6265, rs988748, rs11030104, 11757G/C and rs7103411), which are all SNPs with previously demonstrated clinical relevance to schizophrenia and depression. The present technique therefore represents a suitable protocol for many research laboratories to study the genetic correlates of BDNF in psychiatric disorders. Copyright Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmacology, University of British Columbia, Vancouver, Canada.
authors.forename: Cathy K
authors.initials: CK
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, University of British Columbia, Vancouver, Canada.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Xu
authors.affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Ross
authors.affiliation: Department of Pharmacology, University of British Columbia, Vancouver, Canada.
authors.forename: Ryan
authors.initials: R
authors.lastname: Lo
authors.affiliation: Department of Psychiatry, University of British Columbia, Vancouver, Canada.|British Columbia Mental Health & Addictions Research Institute, Vancouver, Canada.
authors.forename: Ric M
authors.initials: RM
authors.lastname: Procyshyn
authors.affiliation: Department of Psychiatry, University of British Columbia, Vancouver, Canada.|British Columbia Mental Health & Addictions Research Institute, Vancouver, Canada.
authors.forename: Fidel
authors.initials: F
authors.lastname: Vila-Rodriguez
authors.affiliation: Department of Psychiatry, University of British Columbia, Vancouver, Canada.
authors.forename: Randall F
authors.initials: RF
authors.lastname: White
authors.affiliation: Department of Psychiatry, University of British Columbia, Vancouver, Canada.|British Columbia Mental Health & Addictions Research Institute, Vancouver, Canada.
authors.forename: William G
authors.initials: WG
authors.lastname: Honer
authors.affiliation: Department of Pharmacology, University of British Columbia, Vancouver, Canada.|British Columbia Mental Health & Addictions Research Institute, Vancouver, Canada.
authors.forename: Alasdair M
authors.initials: AM
authors.lastname: Barr
mesh_terms: D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000191:economics* / Q000379:methods*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D001523:Mental Disorders / Q000150:complications / Q000235:genetics*; D009422:Nervous System Diseases / Q000150:complications / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D019208:Brain-Derived Neurotrophic Factor
keywords: SNP genotyping; T-ARMS; brain-derived neurotrophic factor; psychiatric disorders; schizophrenia