title: miR-448 negatively regulates ovarian cancer cell growth and metastasis by targeting CXCL12.
abstract: OBJECTIVE
  The purpose of the study is to investigate the roles of miR-448 in ovarian cancer.
  
  
  METHODS
  miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber.
  
  
  RESULTS
  miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression.
  
  
  CONCLUSIONS
  Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.
authors.affiliation: Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China. lv_yuefeng@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China.
authors.forename: W
authors.initials: W
authors.lastname: Ding
authors.affiliation: Department of Gynaecology, Shiyan Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Gynaecology, Shiyan Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: C
authors.initials: C
authors.lastname: Fang
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics; D054377:Chemokine CXCL12 / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014161:Transduction, Genetic
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C557400:MIRN448 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: CXCL12; Metastasis; Ovarian cancer; Proliferation; miR-448