title: Selenorhodamine photosensitizers with the Texas-red core for photodynamic therapy of cancer cells.
abstract: We examined two selenorhodamines with amide and thioamide functionality at the 5-position of a 9-(2-thienyl) substituent on the selenoxanthylium analogue of the Texas-red core for their potential as photosensitizers for photodynamic therapy (PDT) in P-glycoprotein (P-gp)-expressing Colo-26 cells. These compounds were examined for their photophysical properties (absorption, fluorescence, and ability to generate singlet oxygen), for their uptake into Colo-26 cells in the absence or presence of verapamil, for their dark and phototoxicity toward Colo-26 cells, and for their co-localization with mitochondrial-specific agents in Colo-26 cells. Both compounds were extremely effective photosensitizers with values of EC50 ⩽ 4 × 10(-8)M toward Colo-26 cells with 1.0 J cm(-2) laser light delivered at 630 ± 2 nm.
authors.affiliation: Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, United States. Electronic address: markkrym@buffalo.edu.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Kryman
authors.affiliation: Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, United States. Electronic address: kdavies@buffalo.edu.
authors.forename: Kellie S
authors.initials: KS
authors.lastname: Davies
authors.affiliation: Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, United States. Electronic address: mklinder@buffalo.edu.
authors.forename: Michelle K
authors.initials: MK
authors.lastname: Linder
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, State University of New York, Buffalo, NY 14260, United States. Electronic address: tyo2@buffalo.edu.
authors.forename: Tymish Y
authors.initials: TY
authors.lastname: Ohulchanskyy
authors.affiliation: Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, United States; Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, State University of New York, Buffalo, NY 14260, United States. Electronic address: mdetty@buffalo.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Detty
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D016566:Organoselenium Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity; D012235:Rhodamines / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity; D026082:Singlet Oxygen / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014966:Xanthenes / Q000737:chemistry*
chemical_list: D016566:Organoselenium Compounds; D017319:Photosensitizing Agents; D012235:Rhodamines; D014966:Xanthenes; D026082:Singlet Oxygen; C034657:Texas red
keywords: P-glycoprotein; Photodynamic therapy; Photosensitizers; Selenorhodamine