title: Role of ultrasound and microbubble-mediated heat shock protein 72 siRNA on ischemia-reperfusion liver injury in rat.
abstract: OBJECTIVE
  To explore the ultrasound-guided gene transfection as well as the role of heat shock protein 72 (HSP72) siRNA combined with ultrasound micro-bubble contrast agents on rat hepatic ischemia-reperfusion injury.
  
  
  METHODS
  72 SD rats were divided into non-surgery group (group N), sham-operation group (group P) and liver ischemia-reperfusion groups (I/R). In each group, rats were further divided into 4 subgroups according to the different intravenous treatment: 220 ul saline solution (group A); 20 ul HSP72 siRNA plasmid vector + 200 ul saline solution (group B); 20 ul HSP72 siRNA plasmid vector + 200 ul ultrasound microbubble contrast agent (group C); 20 ul HSP72 siRNA plasmid vector + 200 ul ultrasound microbubble contrast agent + ultrasonic irradiation target region with MI1.0 (group D).
  
  
  RESULTS
  Certain degree hepatic tissue injury was observed in rats of group I/R A, B and C. The expressions of liver tissue HSP72 mRNA and HSP72 protein and the concentrations of peripheral blood HSP72, ALT and TNF-α were significantly increased at each I/R subgroup (vs group N and group P, P < 0.01). Among them, the plasma concentrations of ALT, HSP72, and TNF-α and the liver tissue expressions of HSP72 mRNA and HSP72 protein at group A were significantly higher than groups B, C and D (P < 0.01). And group D was significantly lower than that of group A, B and C (P < 0.01).
  
  
  CONCLUSIONS
  The liver tissue expressions of HSP72 mRNA and HSP72 protein and the liver injury degree of ischemia-perfusion were significantly reduced after the HSP72 siRNA was combined with micro-bubble and radiated directionally by ultrasound.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Shaoxing University Shaoxing 312000, Zhejiang, China.
authors.forename: Chungen
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of Lab Medicine, Affiliated Hospital of Shaoxing University Shaoxing 312000, Zhejiang, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Shaoxing University Shaoxing 312000, Zhejiang, China.
authors.forename: Danwen
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Ultrasound Diagnosis, Affiliated Hospital of Shaoxing University Shaoxing 312000, Zhejiang, China.
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Xia
keywords: Heat shock protein 72; gene transfection; ischemia-reperfusion liver injury; siRNA; ultrasound micro-bubble contrast agents