title: Antioxidant and antiproliferative activities of polysaccharide fractions from litchi pulp.
abstract: Three litchi polysaccharide fractions (LPFs), LP-4, LP-6 and LP-8, were obtained by fractional precipitation using 40%, 60% and 80% ethanol, respectively. The physicochemical properties, chemical antioxidant, and cellular antioxidant and antiproliferative activities of the three polysaccharide fractions were compared. LP-6 contained the highest contents of uronic acid and binding protein among the three fractions, whereas LP-8 contained the least. Amino acid composition analysis of the binding protein revealed that LP-6 contained the most acidic and aromatic amino acids. However, LP-8 contained more galactose and mannose than LP-4 and LP-6. LP-6 exhibited the highest chemical antioxidant activities, with an oxygen radical absorbance capacity of 28.14 μmol TE per g DW. LP-8 exhibited higher cellular antioxidant activity and a greater inhibitory effect on the proliferation of A549, HepG2 and MGC-803 cells at a concentration of 100-800 μg mL(-1) than LP-4 and LP-6. In summary, the different LPFs exhibited different antioxidant and antiproliferative activities with differential physicochemical properties.
authors.affiliation: Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, P. R. China. mwzhh@vip.tom.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.forename: Ruifen
authors.initials: R
authors.lastname: Zhang
authors.forename: Lihong
authors.initials: L
authors.lastname: Dong
authors.forename: Jinxin
authors.initials: J
authors.lastname: Guo
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Deng
authors.forename: Yang
authors.initials: Y
authors.lastname: Yi
authors.forename: Mingwei
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D032125:Litchi / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts