title: Levels of Ancylostoma infections and phylogenetic analysis of cox 1 gene of A. ceylanicum in stray cat faecal samples from Guangzhou, China.
abstract: Ancylostoma ceylanicum is a common zoonotic nematode. Cats act as natural reservoirs of the hookworm and are involved in transmitting infection to humans, thus posing a potential risk to public health. The prevalence of feline A. ceylanicum in Guangzhou (South China) was surveyed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In total, 112 faecal samples were examined; 34.8% (39/112) and 43.8% (49/112) samples were positive with hookworms by microscopy and PCR method, respectively. Among them, 40.8% of samples harboured A. ceylanicum. Twelve positive A. ceylanicum samples were selected randomly and used for cox 1 sequence analysis. Sequencing results revealed that they had 97-99% similarity with A. ceylanicum cox 1 gene sequences deposited in GenBank. A phylogenetic tree showed that A. ceylanicum isolates were divided into two groups: one comprising four isolates from Guangzhou (South China), and the other comprising those from Malaysia, Cambodia and Guangzhou. In the latter group, all A. ceylanicum isolates from Guangzhou were clustered into a minor group again. The results indicate that the high prevalence of A. ceylanicum in stray cats in South China poses a potential risk of hookworm transmission from pet cats to humans, and that A. ceylanicum may be a species complex worldwide.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: X G
authors.initials: XG
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: L P
authors.initials: LP
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: M R
authors.initials: MR
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: A Y
authors.initials: AY
authors.lastname: Abdulahi
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University,Guangzhou, Guangdong Province,510642,People's Republic of China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Li
mesh_terms: D000722:Ancylostoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000724:Ancylostomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D051545:Cyclooxygenase 1 / Q000235:genetics*; D005243:Feces / Q000469:parasitology*; D014644:Genetic Variation; D005838:Genotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D017422:Sequence Analysis, DNA
chemical_list: D051545:Cyclooxygenase 1