title: Validity of Tissue Microarray by Immunohistochemistry.
abstract: BACKGROUND
  Tissue microarrays (TMAs) extract designated areas of tissue paraffin blocks in several units that are millimeters in diameter in a cylindrical fashion, array dozens of these tissue specimens, and then re-embed them. Here, a TMA was utilized to analyze renal cell carcinoma (RCC) specimens with anti-FABP7 and anti-Brn2 antibodies.
  
  
  METHODS
  Paraffin-embedded specimens from 114 RCC patients were immunostained with anti-FABP7 and anti-Brn2 antibodies to examine the rate of agreement between the staining of TMA grafts compared to conventional tissue slice grafts. The staining area of the tumor was also examined.
  
  
  RESULTS
  The positive ratio of anti-FABP7 was 74% and of anti-Brn2 was 57%. The rate of agreement of each antibody was 100% regardless of tumor size before extraction.
  
  
  CONCLUSIONS
  Immunostaining of TMA slices might be effective for the analysis of RCC specimens.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Fujita
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Takayama
authors.forename: Naohisa
authors.initials: N
authors.lastname: Takaoka
authors.forename: Cheng
authors.initials: C
authors.lastname: Tan
authors.forename: Hisaki
authors.initials: H
authors.lastname: Igarashi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Ozono
mesh_terms: D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis*; D000071438:Fatty Acid-Binding Protein 7; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000473:pathology; D046888:Tissue Array Analysis / Q000379:methods*; D025521:Tumor Suppressor Proteins / Q000032:analysis*
chemical_list: D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D025521:Tumor Suppressor Proteins