title: Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease.
abstract: The microbial community of the reproductive appara-tus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic infor-mation about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal surface mucosal secretions were col-lected from five healthy cows and five cows that showed symptoms of reproductive disorders. Following high-throughput sequencing of the isolated microbial DNA, data were processed using the Mothur soft-ware to remove low-quality sequences and chimeras, and released to the Ribosomal Database Project for classification of operational taxo-nomic units (OTUs). Local BLASTn was performed and results were loaded into the MEGAN program for viewing profiles and taxonomic microbial attributes. The control profile comprised a total of 15 taxa, with Bacteroides, Enterobacteriaceae, and Victivallis comprising the highest representation of OTUs; the reproductive disorder-positive profile comprised 68 taxa, with Bacteroides, Enterobacteriaceae, His-tophilus, Victivallis, Alistipes, and Coriobacteriaceae being the taxa with the most OTU representation. A change was observed in both the community composition as well as in the microbial attributes of the profiles, suggesting that a relationship might exist between the patho-gen and representative taxa, reflecting the production of metabolites to disease progression.
authors.affiliation: Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil nureyevrod@gmail.com.
authors.forename: N F
authors.initials: NF
authors.lastname: Rodrigues
authors.affiliation: Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Kästle
authors.affiliation: Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: T J D
authors.initials: TJ
authors.lastname: Coutinho
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: A T
authors.initials: AT
authors.lastname: Amorim
authors.affiliation: Núcleo de Tecnologia em Saúde, Instituto Multidisciplinar em Saúde, Campus Anísio Teixeira, Universidade Federal da Bahia, Vitória da Conquista, BA, Brasil.
authors.forename: G B
authors.initials: GB
authors.lastname: Campos
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: V M
authors.initials: VM
authors.lastname: Santos
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Marques
authors.affiliation: Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Timenetsky
authors.forename: S T
authors.initials: ST
authors.lastname: de Farias
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D002417:Cattle; D005260:Female; D056186:Metagenomics; D064307:Microbiota / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060737:Reproductive Tract Infections / Q000382:microbiology* / Q000662:veterinary; D014621:Vagina / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S