title: Annotation of the Protein Coding Regions of the Equine Genome.
abstract: Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine reference genome, since they are also identified as homozygous variants by genomic DNA resequencing of the reference horse. Taken together, we provide a resource of equine mRNA structures and protein coding variants that will enhance equine and cross-species transcriptional and genomic comparisons.
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Hestand
authors.affiliation: Biochemistry and Molecular Biology Department, School of Medicine, University of Louisville, Louisville, Kentucky, United States of America.
authors.forename: Theodore S
authors.initials: TS
authors.lastname: Kalbfleisch
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Coleman
authors.affiliation: Department of Computer Science, University of Kentucky, Lexington, Kentucky, United States of America.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Computer Science, University of Kentucky, Lexington, Kentucky, United States of America.
authors.forename: Jinze
authors.initials: J
authors.lastname: Liu
authors.affiliation: Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Orlando
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.
authors.forename: James N
authors.initials: JN
authors.lastname: MacLeod
mesh_terms: D000818:Animals; D001483:Base Sequence; D016678:Genome; D006736:Horses / Q000235:genetics*; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger