title: Effect of different organic matters on flocculation of Chlorella sorokiniana and optimization of flocculation conditions in swine manure wastewater.
abstract: In this study, flocculation of Chlorella sorokiniana cultivated in swine manure wastewater, BG-11 medium and BG-11 medium supplemented with different organic matters (glucose, urea and tryptone) was investigated. The results demonstrated that the minimum amount of Al(3+) required for complete flocculation in wastewater would increase substantially, and flocculation efficiency became highly sensitive to pH. Tryptone could cause similar extent of inhibition on flocculation as in wastewater. Meanwhile, glucose could increase concentrations of Algogenic Organic Matter (AOM), inhibiting flocculation strongly at higher pH, including flocculation induced by Al(3+) and autoflocculation. However, urea had little effect on flocculation of C. sorokiniana. Moreover, the major factors: dilution times, pH and flocculants dosage, which had significant impact on flocculation efficiency of C. sorokiniana in piggery wastewater, were optimized using response surface methodology (RSM). The optimal flocculation efficiency (100%) was achieved at pH 8.5, 7-folds of dilution and 52.14 mg L(-1) of Al(3+).
authors.affiliation: State Key Laboratory for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: chensf@cau.edu.cn.
authors.forename: Sanfeng
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D002449:Cell Aggregation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002708:Chlorella / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D003198:Computer Simulation; D007220:Industrial Waste / Q000517:prevention & control; D008372:Manure / Q000382:microbiology*; D008954:Models, Biological; D009930:Organic Chemicals / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity; D013552:Swine; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D007220:Industrial Waste; D008372:Manure; D009930:Organic Chemicals; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Chlorella; Flocculation; Organic matters; Response surface methodology (RSM); Swine manure wastewater