title: Curcumin enhanced cholesterol efflux by upregulating ABCA1 expression through AMPK-SIRT1-LXRα signaling in THP-1 macrophage-derived foam cells.
abstract: Curcumin, a traditional Chinese derivative from the rhizomes of Curcuma longa, is beneficial to health by modulating lipid metabolism and suppressing atherogenesis. A key part of atherosclerosis is the failure of macrophages to restore their cellular cholesterol homeostasis and the formation of foam cells. In this study, results showed that curcumin dramatically increased the expression of ATP-binding cassette transporter 1 (ABCA1), promoted cholesterol efflux from THP-1 macrophage-derived foam cells, and reduced cellular cholesterol levels. Curcumin activated AMP-activated protein kinase (AMPK) and SIRT1, and then activated LXRα in THP-1 macrophage-derived foam cells. Inhibiting AMPK/SIRT1 activity by its specific inhibitor or by small interfering RNA could inhibit LXRα activation and abolish curcumin-induced ABCA1 expression and cholesterol efflux. Thus, curcumin enhanced cholesterol efflux by upregulating ABCA1 expression through activating AMPK-SIRT1-LXRα signaling in THP-1 macrophage-derived foam cells. This study describes a possible mechanism for understanding the antiatherogenic effects of curcumin on attenuating the progression of atherosclerosis.
authors.affiliation: 1 Department of Pathology, Affiliated Hui Zhou Hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University Huizhou City , Huizhou, People's Republic of China .|2 Key Laboratory for Atherosclerology of Hunan Province, Institute of Cardiovascular Disease, University of South China , Hengyang, People's Republic of China .
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Lin
authors.affiliation: 3 Department of Clinical Laboratory, Affiliated Nanhua Hospital, University of South China , Hengyang, People's Republic of China .
authors.forename: Mi-Hua
authors.initials: MH
authors.lastname: Liu
authors.affiliation: 1 Department of Pathology, Affiliated Hui Zhou Hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University Huizhou City , Huizhou, People's Republic of China .
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Hu
authors.affiliation: 4 Department of Ultrasonic Diagnosis, Affiliated First Hospital, Hebei Medical University , Shi Jiazhuang, People's Republic of China .
authors.forename: Hong-ru
authors.initials: HR
authors.lastname: Feng
authors.affiliation: 1 Department of Pathology, Affiliated Hui Zhou Hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University Huizhou City , Huizhou, People's Republic of China .
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Fan
authors.affiliation: 1 Department of Pathology, Affiliated Hui Zhou Hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University Huizhou City , Huizhou, People's Republic of China .
authors.forename: Wei-wen
authors.initials: WW
authors.lastname: Zou
authors.affiliation: 1 Department of Pathology, Affiliated Hui Zhou Hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University Huizhou City , Huizhou, People's Republic of China .
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Pan
authors.affiliation: 1 Department of Pathology, Affiliated Hui Zhou Hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University Huizhou City , Huizhou, People's Republic of China .
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Hu
authors.affiliation: 2 Key Laboratory for Atherosclerology of Hunan Province, Institute of Cardiovascular Disease, University of South China , Hengyang, People's Republic of China .
authors.forename: Zuo
authors.initials: Z
authors.lastname: Wang
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics / Q000378:metabolism*; D000263:Adenylate Kinase / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism*; D003474:Curcumin / Q000494:pharmacology*; D005487:Foam Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000378:metabolism; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000960:Hypolipidemic Agents; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; D057093:Orphan Nuclear Receptors; D002784:Cholesterol; D000263:Adenylate Kinase; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D003474:Curcumin