title: Investigation of Optical Coherence Microelastography as a Method to Visualize Cancers in Human Breast Tissue.
abstract: An accurate intraoperative identification of malignant tissue is a challenge in the surgical management of breast cancer. Imaging techniques that help address this challenge could contribute to more complete and accurate tumor excision, and thereby help reduce the current high reexcision rates without resorting to the removal of excess healthy tissue. Optical coherence microelastography (OCME) is a three-dimensional, high-resolution imaging technique that is sensitive to microscale variations of the mechanical properties of tissue. As the tumor modifies the mechanical properties of breast tissue, OCME has the potential to identify, on the microscale, involved regions of fresh, unstained tissue. OCME is based on the use of optical coherence tomography (OCT) to measure tissue deformation in response to applied mechanical compression. In this feasibility study on 58 ex vivo samples from patients undergoing mastectomy or wide local excision, we demonstrate the performance of OCME as a means to visualize tissue microarchitecture in benign and malignant human breast tissues. Through a comparison with corresponding histology and OCT images, OCME is shown to enable ready visualization of features such as ducts, lobules, microcysts, blood vessels, and arterioles and to identify invasive tumor through distinctive patterns in OCME images, often with enhanced contrast compared with OCT. These results lay the foundation for future intraoperative studies. Cancer Res; 75(16); 3236-45. ©2015 AACR.
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia. brendan.kennedy@uwa.edu.au.
authors.forename: Brendan F
authors.initials: BF
authors.lastname: Kennedy
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Robert A
authors.initials: RA
authors.lastname: McLaughlin
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Kelsey M
authors.initials: KM
authors.lastname: Kennedy
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Lixin
authors.initials: L
authors.lastname: Chin
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Wijesinghe
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Curatolo
authors.affiliation: School of Surgery, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Alan
authors.initials: A
authors.lastname: Tien
authors.affiliation: Breast Clinic, Royal Perth Hospital, Perth, Western Australia, Australia.
authors.forename: Maxine
authors.initials: M
authors.lastname: Ronald
authors.affiliation: PathWest, Perth, Western Australia, Australia.
authors.forename: Bruce
authors.initials: B
authors.lastname: Latham
authors.affiliation: School of Surgery, The University of Western Australia, Crawley, Western Australia, Australia. Breast Clinic, Royal Perth Hospital, Perth, Western Australia, Australia.
authors.forename: Christobel M
authors.initials: CM
authors.lastname: Saunders
authors.affiliation: Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia. Centre for Microscopy, Characterization and Analysis, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: David D
authors.initials: DD
authors.lastname: Sampson
mesh_terms: D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis*; D054459:Elasticity Imaging Techniques / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*