title: Dual Action of Myricetin on Porphyromonas gingivalis and the Inflammatory Response of Host Cells: A Promising Therapeutic Molecule for Periodontal Diseases.
abstract: Periodontitis that affects the underlying structures of the periodontium, including the alveolar bone, is a multifactorial disease, whose etiology involves interactions between specific bacterial species of the subgingival biofilm and the host immune components. In the present study, we investigated the effects of myricetin, a flavonol largely distributed in fruits and vegetables, on growth and virulence properties of Porphyromonas gingivalis as well as on the P. gingivalis-induced inflammatory response in host cells. Minimal inhibitory concentration values of myricetin against P. gingivalis were in the range of 62.5 to 125 μg/ml. The iron-chelating activity of myricetin may contribute to the antibacterial activity of this flavonol. Myricetin was found to attenuate the virulence of P. gingivalis by reducing the expression of genes coding for important virulence factors, including proteinases (rgpA, rgpB, and kgp) and adhesins (fimA, hagA, and hagB). Myricetin dose-dependently prevented NF-κB activation in a monocyte model. Moreover, it inhibited the secretion of IL-6, IL-8 and MMP-3 by P. gingivalis-stimulated gingival fibroblasts. In conclusion, our study brought clear evidence that the flavonol myricetin exhibits a dual action on the periodontopathogenic bacterium P. gingivalis and the inflammatory response of host cells. Therefore, myricetin holds promise as a therapeutic agent for the treatment/prevention of periodontitis.
authors.affiliation: Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Grenier
authors.affiliation: Department of Stomatology, Hubei University of Science and Technology, Xianning City, Hubei Province, China.
authors.forename: Huangqin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Amel
authors.initials: A
authors.lastname: Ben Lagha
authors.affiliation: Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Jade
authors.initials: J
authors.lastname: Fournier-Larente
authors.affiliation: Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Morin
mesh_terms: D017364:Collagenases / Q000378:metabolism; D003124:Colorimetry; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005881:Gingiva / Q000473:pathology; D054884:Host-Pathogen Interactions / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000382:microbiology*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D016328:NF-kappa B / Q000378:metabolism; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000382:microbiology* / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017262:Siderophores / Q000378:metabolism; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005419:Flavonoids; D015850:Interleukin-6; D016209:Interleukin-8; D007502:Iron Chelating Agents; D016328:NF-kappa B; D012333:RNA, Messenger; D017262:Siderophores; D037521:Virulence Factors; C040015:myricetin; D017364:Collagenases; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9