title: Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration.
abstract: Extracellular matrix plays a dynamic role during the process of wound healing, embryogenesis and tissue regeneration. Caudal fin regeneration in zebrafish is an excellent model to study tissue and skeletal regeneration. We have analyzed the expression pattern of some of the well characterized ECM proteins during the process of caudal fin regeneration in zebrafish. Our results show that a transitional matrix analogous to the one formed during newt skeletal and heart muscle regeneration is synthesized during fin regeneration. Here we demonstrate that a provisional matrix rich in hyaluronic acid, tenascin C, and fibronectin is synthesized following amputation. Additionally, we observed that the link protein Hapln1a dependent ECM, consisting of Hapln1a, hyaluronan and proteoglycan aggrecan, is upregulated during fin regeneration. Laminin, the protein characteristic of differentiated tissues, showed only modest change in the expression pattern. Our findings on zebrafish fin regeneration implicates that changes in the extracellular milieu represent an evolutionarily conserved mechanism that proceeds during tissue regeneration, yet with distinct players depending on the type of tissue that is involved.
authors.affiliation: Department of Biological Sciences, Lehigh University, USA.
authors.forename: Jayalakshmi
authors.initials: J
authors.lastname: Govindan
authors.affiliation: Department of Biological Sciences, Lehigh University, USA. Electronic address: mki3@lehigh.edu.
authors.forename: M Kathryn
authors.initials: MK
authors.lastname: Iovine
mesh_terms: D058500:Animal Fins / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D019063:Tenascin / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology; D015027:Zebrafish / Q000378:metabolism / Q000502:physiology*; D029961:Zebrafish Proteins / Q000378:metabolism; D000099249:Proteoglycan Link Protein
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006820:Hyaluronic Acid; D011509:Proteoglycans; D019063:Tenascin; D029961:Zebrafish Proteins; C000594258:Hapln1a protein, zebrafish; D000099249:Proteoglycan Link Protein
keywords: Aggrecan; ECM; Fibronectin; Hapln1a; Hyaluronic acid; Regeneration; Tenascin C; Transitional matrix; Zebrafish