title: Phthalocyanine-mediated Photodynamic Treatment of Tumoural and Non-tumoural cell lines.
abstract: This study deals with the use of cationic far-red absorbing photosensitizers (λ(max) ~740 nm) from the group of the phthalocyanines, in photodynamic therapy. The photosensitizers differed in their central atom, bearing either hydrogen, zinc or magnesium. These photosensitizers were tested in vitro on the tumour cell line HeLa (cervical cancer) and non-tumour cell line NIH3T3 (mouse fibroblast). The following tests were performed: measurement of reactive oxygen species production, viability testing, Comet assay and cell type detection (apoptotic, necrotic and living cells). The best results were achieved with zinc derivative at relatively low half-maximum inhibitory concentration (0.04 μM) and a total radiation dose of 15 J cm(-2).
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic barbora.manisova@upol.cz.
authors.forename: Barbora
authors.initials: B
authors.lastname: Manisova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: Svatopluk
authors.initials: S
authors.lastname: Binder
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Malina
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Jiravova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Langova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kolarova
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology
chemical_list: D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D008274:Magnesium; D015032:Zinc; C013647:phthalocyanine
keywords: Photosensitizer; Phthalocyanine; cellular damage; oxidative stress; photodynamic treatment; phototoxicity; tumour cells