title: Autologous mobilized peripheral blood CD34(+) cell infusion in non-viral decompensated liver cirrhosis.
abstract: OBJECTIVE
  To study the effect of mobilized peripheral blood autologous CD34 positive (CD34(+)) cell infusion in patients with non-viral decompensated cirrhosis.
  
  
  METHODS
  Cirrhotic patients of non-viral etiology were divided into 2 groups based on their willingness to be listed for deceased donor liver transplant (DDLT) (control, n = 23) or to receive autologous CD34(+) cell infusion through the hepatic artery (study group, n = 22). Patients in the study group were admitted to hospital and received granulocyte colony stimulating factor injections 520 μg/d for 3 consecutive days to mobilize CD34(+) cells from the bone marrow. On day 4, leukapheresis was done and CD34(+) cells were isolated using CliniMAC magnetic cell sorter. The isolated CD34(+) cells were infused into the hepatic artery under radiological guidance. The patients were discharged within 48 h. The control group received standard of care treatment for liver cirrhosis and were worked up for DDLT as per protocol of the institute. Both groups were followed up every week for 4 wk and then every month for 3 mo.
  
  
  RESULTS
  In the control and the study group, the cause of cirrhosis was cryptogenic in 18 (78.2%) and 16 (72.72%) and alcohol related in 5 (21.7%) and 6 (27.27%), respectively. The mean day 3 cell count (cells/μL) was 27.00 ± 20.43 with a viability of 81.84 ± 11.99%. and purity of 80%-90%. Primary end point analysis revealed that at 4 wk, the mean serum albumin in the study group increased significantly (2.83 ± 0.36 vs 2.43 ± 0.42, P = 0.001) when compared with controls. This improvement in albumin was, however, not sustained at 3 mo. However, at the end of 3 mo there was a statistically significant improvement in serum creatinine in the study group (0.96 ± 0.33 vs 1.42 ± 0.70, P = 0.01) which translated into a significant improvement in the Model for End-Stage Liver Disease score (15.75 ± 5.13 vs 19.94 ± 6.68, P = 0.04). On statistical analysis of secondary end points, the transplant free survival at the end of 1 mo and 3 mo did not show any significant difference (P = 0.60) when compared to the control group. There was no improvement in aspartate transaminase, alanine transaminase, and bilirubin at any point in the study population. There was no mortality benefit in the study group. The procedure was safe with no procedural or treatment related complications.
  
  
  CONCLUSIONS
  Autologous CD 34(+) cell infusion is safe and effectively improves liver function in the short term and may serve as a bridge to liver transplantation.
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Mithun
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Padaki Nagaraja
authors.initials: PN
authors.lastname: Rao
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Mitnala
authors.initials: M
authors.lastname: Sasikala
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Mamata Reddy
authors.initials: MR
authors.lastname: Kuncharam
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Chimpa
authors.initials: C
authors.lastname: Reddy
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Vardaraj
authors.initials: V
authors.lastname: Gokak
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Bpss
authors.initials: B
authors.lastname: Raju
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Jagdeesh R
authors.initials: JR
authors.lastname: Singh
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: Piyal
authors.initials: P
authors.lastname: Nag
authors.affiliation: Mithun Sharma, Padaki Nagaraja Rao, Vardaraj Gokak, BPSS Raju, D Nageshwar Reddy, Department of Gastroenterology and Hepatology, Asian Institute of Gastroenterology, Asian Healthcare Foundation, Hyderabad 500082, India.
authors.forename: D
authors.initials: D
authors.lastname: Nageshwar Reddy
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000276:immunology*; D006499:Hepatic Artery; D006801:Humans; D018189:Immunomagnetic Separation; D007194:India; D019934:International Normalized Ratio; D007937:Leukapheresis; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis / Q000401:mortality / Q000601:surgery*; D008111:Liver Function Tests; D008115:Liver Regeneration; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects / Q000401:mortality; D020127:Recovery of Function; D012709:Serum Albumin / Q000378:metabolism; D000075462:Serum Albumin, Human; D013997:Time Factors; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: C422455:ALB protein, human; D018952:Antigens, CD34; D015415:Biomarkers; D012709:Serum Albumin; D016179:Granulocyte Colony-Stimulating Factor; D000075462:Serum Albumin, Human
keywords: CD34 cell infusion; Cirrhosis; Liver transplantation; Model for end-stage liver disease; Stem cell