title: The antioxidant effect of astaxanthin is higher in young mice than aged: a region specific study on brain.
abstract: Astaxanthin is a potential antioxidant which shows neuroprotective property. We aimed to investigate the age-dependent and region-specific antioxidant effects of astaxanthin in mice brain. Animals were divided into 4 groups; treatment young (3 months, n = 6) (AY), treatment old (16 months, n = 6) (AO), placebo young (3 months, n = 6) (PY) and placebo old (16 months, n = 6) (PO) groups. Treatment group was given astaxanthin (2 mg/kg/day, body weight), and placebo group was given 100 μl of 0.9% normal saline orally to the healthy Swiss albino mice for 4 weeks. The level of non-enzymatic oxidative markers namely malondialdehyde (MDA); nitric oxide (NO); advanced protein oxidation product (APOP); glutathione (GSH) and the activity of enzymatic antioxidants i.e.; catalase (CAT) and superoxide dismutase (SOD) were determined from the isolated brain regions. Treatment with astaxanthin significantly (p < 0.05) reduces the level of MDA, APOP, NO in the cortex, striatum, hypothalamus, hippocampus and cerebellum in both age groups. Astaxanthin markedly (p < 0.05) enhances the activity of CAT and SOD enzymes while improves the level of GSH in the brain. Overall, improvement of oxidative markers was significantly greater in the young group than the aged animal. In conclusion, we report that the activity of astaxanthin is age-dependent, higher in young in compared to the aged brain.
authors.affiliation: Department of Pharmaceutical Sciences, North South University, Plot 15, Block B, Bashundhara, Dhaka, 1229, Bangladesh, bd_pharmacy@yahoo.com.
authors.forename: Md Mamun
authors.initials: MM
authors.lastname: Al-Amin
authors.forename: Samiha
authors.initials: S
authors.lastname: Akhter
authors.forename: Ahmed Tasdid
authors.initials: AT
authors.lastname: Hasan
authors.forename: Tanzir
authors.initials: T
authors.lastname: Alam
authors.forename: S M
authors.initials: SM
authors.lastname: Nageeb Hasan
authors.forename: A R M
authors.initials: AR
authors.lastname: Saifullah
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shohel
mesh_terms: D000367:Age Factors; D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D024341:Xanthophylls / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D024341:Xanthophylls; C005948:astaxanthine