title: Preparation and Characterization of Monomodal Grapevine Virus A Capsid Protein.
abstract: Grapevine virus A (GVA), a flexible filament of approximately 800 nm in length is composed of capsid subunits that spontaneously assembles around a positive sense genomic RNA. In addition to encapsidation, plant viruses capsid proteins (CPs) participate in other processes throughout infection and GVA CP is involved in cell-to-cell translocation of the virus. A protocol was developed to obtain low-molecular weight GVA-CP that is not prone to aggregation and spontaneous assembly and this was characterized by circular dichroism and dynamic light scattering. These results indicate the suitably of GVA-CP for X-ray crystallographic and NMR studies that should lead to the elucidation of the first three-dimensional structure of a flexible filamentous virus from the Betaflexiviridae family.
authors.forename: Vinícius S
authors.initials: VS
authors.lastname: Santana
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Mariutti
authors.forename: Raphael J
authors.initials: RJ
authors.lastname: Eberle
authors.forename: Anwar
authors.initials: A
authors.lastname: Ullah
authors.forename: Icaro P
authors.initials: IP
authors.lastname: Caruso
authors.affiliation: Centro Multiusuário de Inovação Biomolecular, Departamento de Física, Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo 2265, São Jose do Rio Preto-SP, 15054-000, Brazil. arni@sjrp.unesp.br.
authors.forename: Raghuvir K
authors.initials: RK
authors.lastname: Arni
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000067493:Dynamic Light Scattering; D052677:Flexiviridae / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins