title: Development of response surface methodology for optimization of extraction parameters and quantitative estimation of embelin from Embelia ribes Burm by high performance liquid chromatography.
abstract: BACKGROUND
  Embelia ribes Burm is widely used medicinal plant for the treatment of different types of disorders in the Indian traditional systems of medicine.
  
  
  OBJECTIVE
  The present work was aimed to optimize the extraction parameters of embelin from E. ribes fruits and also to quantify embelin content in different extracts of the plant.
  
  
  METHODS
  Optimization of extraction parameters such as solvent: drug ratio, temperature and time were carried out by response surface methodology (RSM). Quantitative estimation of embelin in different extracts of E. ribes fruits was done through high performance liquid chromatography.
  
  
  RESULTS
  The optimal conditions determined for extraction of embelin through RSM were; extraction time (27.50 min), extraction temperature 45°C and solvent: drug ratio (8:1). Under the optimized conditions, the embelin yield (32.71%) was equitable to the expected yield (31.07%, P > 0.05). These results showed that the developed model is satisfactory and suitable for the extraction process of embelin. The analysis of variance showed a high goodness of model fit and the accomplishment of the RSM method for improving embelin extraction from the fruits of E. ribes.
  
  
  CONCLUSIONS
  It is concluded that this may be a useful method for the extraction and quantitative estimation of embelin from the fruits of E. ribes.
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.
authors.forename: Md Shamsir
authors.initials: MS
authors.lastname: Alam
authors.affiliation: Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Zoheir A
authors.initials: ZA
authors.lastname: Damanhouri
authors.affiliation: Department of Health Information Technology, Jeddah Community College, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.
authors.forename: Lubna
authors.initials: L
authors.lastname: Abidin
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Amir
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Aqil
authors.affiliation: Department of Pharmacy, Oman Medical College, Muscat, Oman.
authors.forename: Shah Alam
authors.initials: SA
authors.lastname: Khan
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Mujeeb
keywords: Embelia ribes; response surface methodology; reverse phase-high performance liquid chromatography; ultrasonic-assisted extraction