title: Establishment and characterization of a reconstructed Chinese human epidermis model.
abstract: OBJECTIVE
  In vitro reconstructed human epidermis is a powerful tool for both basic research and industrial applications in dermatology, pharmacology and the cosmetic field.
  
  
  METHODS
  By growing keratinocytes of Chinese origin on a collagen matrix after a submerged culture followed by an air-liquid interface culture, an in vitro reconstructed Chinese human epidermis model was obtained. This Chinese epidermis model was further characterized.
  
  
  RESULTS
  The reconstructed human epidermis model (China EpiSkin model) exhibits morphological features similar to native skin and shows similar expression profile of proliferation (Ki67) and differentiation (K14 and K10 cytokeratins, filaggrin) markers. Corneodesmosomes, lamellar lipids, desmosomes, keratohyalin granules, keratin filaments and membrane-coating granules are also observed at the ultrastructure level. Moreover, China EpiSkin model contains most of the major lipid classes normally found in the native skin and potentially could present the properties of skin barrier. More importantly, the model production achieves high reproducibility and low intra- and inter-batch variations.
  
  
  CONCLUSIONS
  This is the first reconstructed Chinese human epidermis model reported to meet the high quality standard with industrialized production criteria. This China EpiSkin model can be used for both skin research and safety assessment in vitro.
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Qiu
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhong
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.|Shanghai EPISKIN Biotechnology Co. Ltd, 1299 Zhang Heng Road, Pudong, Shanghai, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.|Shanghai EPISKIN Biotechnology Co. Ltd, 1299 Zhang Heng Road, Pudong, Shanghai, China.
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.
authors.forename: H
authors.initials: H
authors.lastname: Ni
authors.affiliation: L'Oréal Research and Innovation, 550 Jin Yu Road, Pudong, Shanghai, China.|Shanghai EPISKIN Biotechnology Co. Ltd, 1299 Zhang Heng Road, Pudong, Shanghai, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Cai
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D002681:China; D000078404:Epidermal Cells; D004817:Epidermis / Q000648:ultrastructure; D000091344:Filaggrin Proteins; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D015203:Reproducibility of Results
keywords: Chinese; cell culture; industrialization; keratinocyte; reconstructed human epidermis; skin barrier; skin structure