title: [ITD mutation burden for the prognosis in FLT3-ITD positive acute myeloid leukemia patients].
abstract: OBJECTIVE
  To explore the impact of ITD mutation characteristics on the overall survival (OS) and complete remission duration (CRD) in FLT3-ITD positive non-M3 acute myeloid leukemia (AML).
  
  
  METHODS
  Capillary electrophoresis was used to detect the FLT3-ITD characteristics after PCR amplication. Single or multiple mutations were identified by the numbers of peak. FLT3-ITD mutation burden was calculated by the peak area of mutant divided by the wild-type and mutant peak areas. Clinical data was collected and followed up in the FLT3-ITD mutation patients.
  
  
  RESULTS
  Multiple ITD mutations were common in patients aged 60 and above. Patients with single ITD mutation had higher percentage of blasts in bone marrow than multiple ITD mutations (0.758 vs 0.638, P=0.028). The numbers and length of FLT3-ITD mutation had no impact on prognosis. Patients with less than 10% of ITD mutation burden showed no difference with the intermediate-risk c-kit group in OS and CRD, but the two groups had longer OS and CRD than ITD mutation burden above 10% (OS: undefined, undefined, 9.9 months, P<0.05; CRD: undefined, undefined, 6.7 months, P<0.05). In patients with ITD mutation burden above 10%, cases with NPM1 or CEBPA mutation alone had markedly longer CRD than ITD mutation alone (25.0 vs 5.1 months, P=0.003), while OS were similar (11.4 vs 8.0 months, P>0.05).
  
  
  CONCLUSIONS
  Non-M3 AML patients with less than 10% FLT3-ITD mutation burden had a better prognosis than those above 10%.
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Ding
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Shen
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Zixing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Suning
authors.initials: S
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Jiannong
authors.initials: J
authors.lastname: Cen
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Ding
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou　215006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
mesh_terms: D005838:Genotype; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D009154:Mutation; D000090243:Nucleophosmin; D011379:Prognosis; D012074:Remission Induction; D051941:fms-Like Tyrosine Kinase 3
chemical_list: C000716967:NPM1 protein, human; D000090243:Nucleophosmin; D051941:fms-Like Tyrosine Kinase 3