title: Anti-inflammatory and antioxidant properties of Eriobotrya japonica leaves extracts.
abstract: BACKGROUND
  In the present work we determined phenolic and flavonoids content of Eriobotrya japonica leaves extracts and fractions and their antioxidant and anti-inflammatory properties.
  
  
  OBJECTIVE
  To evaluate the inhibition of inflammatory PLA2 and antioxidant effects of extracts and fractions from Eriobotrya japonica leaves.
  
  
  METHODS
  Antioxidant activity was evaluated with DPPH radical scavenging assay and anti-inflammatory effect of fractions was measured by their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA).
  
  
  RESULTS
  The EtOH/EtOAc 2:1 extract exhibited a potent inhibition of the hG-IIA with an IC50 values of 8 µg/ml. It also shows an antioxidant activity measured on DPPH with an IC50 of 42 µg/ml. Fractionation shows that CH2Cl2/MeOH 0:1 fraction was the rich one on flavonoids compounds (4.3 mg/g dry weight) and demonstrates a high antioxidant activity with an IC50 of 12 µg/ml. The anti-inflammatory evaluation demonstrates that the same fraction was the best one to inhibit the pro-inflammatory phospholipase A2 group IIA with an IC50 of 4 µg/ml.
  
  
  CONCLUSIONS
  Study conducted on Eriobotrya japonica shows that CH2Cl2/MeOH 0:1 fraction inhibits efficiently the hG-IIA phospholipase. which is considered as pro-inflammatory enzyme.
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax BP1173-3038, University of Sfax, Tunisia ; Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax BP1173-3038, University of Sfax, Tunisia.
authors.forename: Kammoun
authors.initials: K
authors.lastname: Maher
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax BP1173-3038, University of Sfax, Tunisia.
authors.forename: Ben Ali
authors.initials: BA
authors.lastname: Yassine
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax BP1173-3038, University of Sfax, Tunisia.
authors.forename: Bezzine
authors.initials: B
authors.lastname: Sofiane
mesh_terms: D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D031983:Eriobotrya / Q000737:chemistry*; D000431:Ethanol; D005419:Flavonoids / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D000431:Ethanol
keywords: Eriobotrya japonica; anti-inflammatory; extraction; flavonoids