title: Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition.
abstract: OBJECTIVE
  To evaluate the heart and the Tc-99m-sestamibi biodistribution after statin pretreatment in a rat model of abdominal sepsis.
  
  
  METHODS
  Twenty-four Wistar rats were randomly distributed into four groups (n=6 per group): 1) sepsis with simvastatin treatment, 2) sepsis with vehicle, 3) sham control with simvastatin and 4) sham control with vehicle. 24 hours after cecal ligation and puncture rats received 1.0MBq of Tc-99m-sestamibi i.v. 30min after, animals were euthanized for ex-vivo tissue counting and myocardium histological analysis.
  
  
  RESULTS
  Myocardial histologic alterations were not detected 24 hours post-sepsis. There was significantly increased cardiac Tc-99m-sestamibi activity in the sepsis group with simvastatin treatment (1.9±0.3%ID/g, p<0.001) in comparison to the sepsis group+vehicle (1.0±0.2%ID/g), control sham group+ simvastatin (1.2±0.3%ID/g) and control sham group (1.3±0.2%ID/g). Significant Tc-99m-sestamibi activity in liver, kidney and lungs was also detected in the sepsis group treated with simvastatinin comparison to the other groups.
  
  
  CONCLUSIONS
  Statin treatment altered the biodistribution of Tc-99m-sestamibi with increased cardiac and solid organ activity in rats with abdominal sepsis, while no impact on controls. Increased myocardial tracer activity may be a result of a possible protection effect due to increased tissue perfusion mediated by statins.
authors.affiliation: Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Robson
authors.initials: R
authors.lastname: Macedo
authors.affiliation: Department of Radiology, Johns Hopkins University, Baltimore, USA.
authors.forename: Som Mehrbod
authors.initials: SM
authors.lastname: Javadi
authors.affiliation: Department of Nuclear Medicine, Wuerzburg University, Wuerzburg, Germany.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Higuchi
authors.affiliation: UFRN, Natal, RN, Brazil.
authors.forename: Marília Daniela Ferreira de
authors.initials: MD
authors.lastname: Carvalho
authors.affiliation: UFRN, Natal, RN, Brazil.
authors.forename: Vanessa de Fátima Lima Paiva
authors.initials: Vde F
authors.lastname: Medeiros
authors.affiliation: Department of Surgery, UFRN, Natal, RN, Brazil.
authors.forename: Ítalo Medeiros
authors.initials: ÍM
authors.lastname: Azevedo
authors.affiliation: Department of Patology, UFRN, Natal, RN, Brazil.
authors.forename: Francisco Pignataro
authors.initials: FP
authors.lastname: Lima
authors.affiliation: Department of Surgery, UFRN, Natal, RN, Brazil.
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006321:Heart / Q000000981:diagnostic imaging / Q000187:drug effects*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000627:therapeutic use*; D059413:Intraabdominal Infections / Q000150:complications / Q000188:drug therapy*; D007668:Kidney / Q000000981:diagnostic imaging / Q000187:drug effects; D008099:Liver / Q000000981:diagnostic imaging / Q000187:drug effects; D008168:Lung / Q000000981:diagnostic imaging / Q000187:drug effects; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D018805:Sepsis / Q000150:complications / Q000188:drug therapy*; D019821:Simvastatin / Q000627:therapeutic use*; D017256:Technetium Tc 99m Sestamibi / Q000493:pharmacokinetics; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi; D019821:Simvastatin