title: Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil.
abstract: A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10(T) was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10(T) belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2-35(T) (91.0%), Spirosoma lingual DSM 74(T) (90.8%), Spirosoma endophyticum EX36(T) (90.7%), Spirosoma panaciterrae DSM 21099(T) (90.5%), Spirosoma rigui WPCB118(T) (90.2%), Spirosoma spitsbergense DSM 19989(T) (89.8%), Spirosoma luteum DSM 19990(T) (89.6%), Spirosoma oryzae RHs22(T) (89.6%), and Spirosoma radiotolerans DG5A(T) (89.1%). Strain DY10(T) showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10(T) were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C15:0, C16:1 ω5c, and summed feature3 (C16:1 ω7c/C16:1 ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10(T) from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10(T) (=KCTC 23999(T) =KEMB 9004-162(T) =JCM 18492(T)).
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kang
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Joo
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
mesh_terms: D000531:Altitude; D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000528:radiation effects; D004275:DNA, Ribosomal / Q000235:genetics; D005720:Gamma Rays; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011836:Radiation Tolerance; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014466:Ultraviolet Rays; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7