title: Characterization and phylogenetic analysis of the swine leukocyte antigen 3 gene from Korean native pigs.
abstract: We investigated the phylogenetic relationships between pig breeds, compared the genetic similarity between humans and pigs, and provided basic genetic information on Korean native pigs (KNPs), using genetic variants of the swine leukocyte antigen 3 (SLA-3) gene. Primers were based on sequences from GenBank (accession Nos. AF464010 and AF464009). Polymerase chain reaction analysis amplified approximately 1727 bp of segments, which contained 1086 bp of coding regions and 641 bp of the 3'- and 5'-untranslated regions. Bacterial artificial chromosome clones of miniature pigs were used for sequencing the SLA-3 genomic region, which was 3114 bp in total length, including the coding (1086 bp) and non-coding (2028 bp) regions. Sequence analysis detected 53 single nucleotide polymorphisms (SNPs), based on a minor allele frequency greater than 0.01, which is low compared with other pig breeds, and the results suggest that there is low genetic variability in KNPs. Comparative analysis revealed that humans possess approximately three times more genetic variation than do pigs. Approximately 71% of SNPs in exons 2 and 3 were detected in KNPs, and exon 5 in humans is a highly polymorphic region. Newly identified sequences of SLA-3 using KNPs were submitted to GenBank (accession No. DQ992512-18). Cluster analysis revealed that KNPs were grouped according to three major alleles: SLA-3*0502 (DQ992518), SLA-3*0302 (DQ992513 and DQ992516), and SLA-3*0303 (DQ992512, DQ992514, DQ992515, and DQ992517). Alignments revealed that humans have a relatively close genetic relationship with pigs and chimpanzees. The information provided by this study may be useful in KNP management.
authors.affiliation: Animal Genomics Bioinformatics Division, National Institute of Animal Science, Suwon, Korea chung133@korea.kr.
authors.forename: H Y
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Animal Genomics Bioinformatics Division, National Institute of Animal Science, Suwon, Korea.
authors.forename: Y C
authors.initials: YC
authors.lastname: Choi
authors.affiliation: Animal Genomics Bioinformatics Division, National Institute of Animal Science, Suwon, Korea.
authors.forename: H N
authors.initials: HN
authors.lastname: Park
mesh_terms: D000818:Animals; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D006649:Histocompatibility Antigens / Q000235:genetics*; D007723:Korea; D010802:Phylogeny; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
chemical_list: D006649:Histocompatibility Antigens