title: Highly carbonylated cellulose nanofibrous membranes utilizing maleic anhydride grafting for efficient lysozyme adsorption.
abstract: Construction of adsorptive materials for simple, efficient, and high-throughput adsorption of proteins is critical to meet the great demands of highly purified proteins in biotechnological and biopharmaceutical industry; however, it has proven extremely challenging. Here, we report a cost-effective strategy to create carbonyl groups surface-functionalized nanofibrous membranes under mild conditions for positively charged protein adsorption. Our approach allows maleic anhydride to in situ graft on cellulose nanofibrous membranes (CMA) to construct adsorptive membranes with large surface area and tortuous porous structure. Thereby, the resultant CMA membranes exhibited high adsorption capacity of 160 mg g(-1), fast equilibrium within 12 h, and good reversibility to lysozyme. Moreover, the dynamic adsorption was performed under low pressure-drops (750 Pa), with a relatively high saturation adsorption amount of 118 mg g(-1), which matched well with the requirements for proteins purification. Considering the excellent adsorption performance of the as-prepared adsorptive membranes, this simple and intriguing approach may pave a way for the design and development of robust and cost-effective adsorption membranes to meet the great demands for fast and efficient adsorption of positively charged proteins.
authors.forename: Juncheng
authors.initials: J
authors.lastname: Ma
authors.affiliation: ‡State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wang
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Fu
authors.affiliation: ‡State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Si
authors.affiliation: §Nanomaterials Research Center, Modern Textile Institute, Donghua University, Shanghai 200051, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Yu
authors.affiliation: ‡State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.|§Nanomaterials Research Center, Modern Textile Institute, Donghua University, Shanghai 200051, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ding
mesh_terms: D000327:Adsorption; D002482:Cellulose / Q000737:chemistry*; D008299:Maleic Anhydrides / Q000737:chemistry*; D008567:Membranes, Artificial; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification; D057139:Nanofibers / Q000737:chemistry*; D016062:Porosity
chemical_list: D008299:Maleic Anhydrides; D008567:Membranes, Artificial; D002482:Cellulose; D009113:Muramidase
keywords: adsorption; electrospun cellulose nanofibrous membranes; lysozyme; maleic anhydride; surface functionalization