title: MicroRNA-34a targets FMNL2 and E2F5 and suppresses the progression of colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common malignancies. Increasing evidences indicate that dysregulation of miRNAs is a frequent event in CRC and contributes to the pathogenesis of CRC. In this study, we found that over-expression of miR-34a inhibited cell proliferation and invasion, induced a cell cycle arrest and triggered apoptosis, while knockdown of miR-34a showed the opposite effects. Moreover, ectopic miR-34a suppressed tumor growth and metastasis of CRC cells in vivo. FMNL2 and E2F5 were identified as direct targets of miR-34a. Reintroduction of FMNL2 or E2F5 without 3'UTR region reversed the inhibitory effects of miR-34a on cell proliferation and invasion. MiR-34a was down-regulated in CRC cells and inversely correlated with FMNL2 and E2F5 expressions. Our study suggests that miR-34a is an important tumor suppressor of CRC progression by targeting FMNL2 and E2F5, thus providing new insight into the molecular mechanisms underlying CRC progression and establishing a strong potential for the application of miR-34a as a novel therapeutic marker against CRC.
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: GuiFeng
authors.initials: G
authors.lastname: Lu
authors.affiliation: Clinical Medical College, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China.
authors.forename: YaLing
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Biostatistics, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China.
authors.forename: ShengLi
authors.initials: S
authors.lastname: An
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: SaiNan
authors.initials: S
authors.lastname: Xin
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: XiaoLi
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: PingXiang
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: WenTing
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: YanQing
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong Province, People's Republic of China. Electronic address: redsnow007@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Liang
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018450:Disease Progression; D015536:Down-Regulation; D050700:E2F5 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D000080865:Formins; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D050700:E2F5 Transcription Factor; C092558:E2F5 protein, human; C476208:FMNL2 protein, human; D000080865:Formins; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D011506:Proteins
keywords: Colorectal cancer; E2F5; FMNL2; MiR-34a; Progression