title: Pheromone-encoding mRNA is transported to the yeast mating projection by specific RNP granules.
abstract: Association of messenger RNAs with large complexes such as processing bodies (PBs) plays a pivotal role in regulating their translation and decay. Little is known about other possible functions of these assemblies. Exposure of haploid yeast cells, carrying mating type "a," to "α pheromone" stimulates polarized growth resulting in a "shmoo" projection; it also induces synthesis of "a pheromone," encoded by MFA2. In this paper, we show that, in response to α pheromone, MFA2 mRNA is assembled with two types of granules; both contain some canonical PB proteins, yet they differ in size, localization, motility, and sensitivity to cycloheximide. Remarkably, one type is involved in mRNA transport to the tip of the shmoo, whereas the other-in local translation in the shmoo. Normal assembly of these granules is critical for their movement, localization, and for mating. Thus, MFA2 mRNAs are transported to the shmoo tip, in complex with PB-like particles, where they are locally translated.
authors.affiliation: Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel Department of Molecular Biology, Ariel University, Ariel 40700, Israel stellar@ariel.ac.il.
authors.forename: Stella
authors.initials: S
authors.lastname: Aronov
authors.affiliation: Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
authors.forename: Saray
authors.initials: S
authors.lastname: Dover-Biterman
authors.affiliation: Multidisciplinary Laboratory, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
authors.forename: Edith
authors.initials: E
authors.lastname: Suss-Toby
authors.affiliation: Multidisciplinary Laboratory, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel Bioinformatics Knowledge Unit, The Lorry I. Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion-Israel Institute of Technology, Haifa 31096, Israel.
authors.forename: Michael
authors.initials: M
authors.lastname: Shmoish
authors.affiliation: Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
authors.forename: Lea
authors.initials: L
authors.lastname: Duek
authors.affiliation: Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
authors.forename: Mordechai
authors.initials: M
authors.lastname: Choder
mesh_terms: D001692:Biological Transport; D021961:Cell Membrane Structures / Q000378:metabolism; D022081:Cell Surface Extensions / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics; D010675:Pheromones / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008074:Lipoproteins; C095063:MFA2 protein, S cerevisiae; D010675:Pheromones; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins