title: PP069. The expressions of B7-H1 and B7-H4 co-stimulatory molecules on myeloid and lymphoid dendritic cells in pre-eclampsia and normal pregnancy. The expressions of B7-H1 and B7-H4 co-stimulatory moleculeson myeloid and lymphoid dendritic cells in pre-eclampsia and normal pregnancy.
abstract: BACKGROUND
  Pre-eclampsia (PE) is a common obstetric syndrome affecting about 5-10% of pregnant women. The syndrome is a leading cause of maternal and fetal morbidity and mortality, even in developed world. The etiology and pathogenesis of this syndrome are not fully understood. There are many studies describing alterations in the innate and adaptive immune system which may have an influence on the onset of this disorder. It was suggested that activation of cell-mediated immunity may play the key role in the etiology of pre-eclampsia.
  
  
  OBJECTIVE
  The purpose of our study was to estimate the expressions of B7-H1 and B7-H4 costimulatory molecules on myeloid and lymphoid DCs (CD1c(+), BDCA-2(+)) in the peripheral blood of patients with pre-eclampsia and normal pregnant women in the third trimesters of physiological pregnancy.
  
  
  METHODS
  Thirty three patients with pre-eclampsia and 26 normal pregnant women were included in the study. Dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies against blood dendritic cell antigens and B7-H1 and B7-H4 molecules and estimated using flow cytometry.
  
  
  RESULTS
  The expressions of B7-H1 molecule on CD1c(+) myeloid DCs and B7-H4 molecule on BDCA-2(+) lymphoid DCs did not differ in pre-eclampsia and healthy third trimester pregnant women. The expressions of B7-H4 molecule on CD1c(+) myeloid DCs and B7-H1 molecule on BDCA-2(+) lymphoid DCs were significantly higher in peripheral blood of patients with pre-eclampsia in comparison with the control group.
  
  
  CONCLUSIONS
  It seems possible that higher expressions of B7-H4 molecule on CD1c(+) myeloid DCs and B7-H1 molecule on lymphoid BDCA-2(+) DCs in pre-eclampsia may be the tolerogenic mechanism secondary to the pro-inflammatory response which is observed in this syndrome.
authors.affiliation: Department of Obstetrics and Perinatology, Lublin, Poland.
authors.forename: D A
authors.initials: DA
authors.lastname: Darmochwal-Kolarz
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kludka-Sternik
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Chmielewski
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Kolarz
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Rolinski
authors.affiliation: Department of Obstetrics and Perinatology, Lublin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Oleszczuk