title: Sox2 promotes survival of satellite glial cells in vitro.
abstract: Sox2 is a transcriptional factor expressed in neural stem cells. It is known that Sox2 regulates cell differentiation, proliferation and survival of the neural stem cells. Our previous study showed that Sox2 is expressed in all satellite glial cells of the adult rat dorsal root ganglion. In this study, to examine the role of Sox2 in satellite glial cells, we establish a satellite glial cell-enriched culture system. Our culture method succeeded in harvesting satellite glial cells with the somata of neurons in the dorsal root ganglion. Using this culture system, Sox2 was downregulated by siRNA against Sox2. The knockdown of Sox2 downregulated ErbB2 and ErbB3 mRNA at 2 and 4 days after siRNA treatment. MAPK phosphorylation, downstream of ErbB, was also inhibited by Sox2 knockdown. Because ErbB2 and ErbB3 are receptors that support the survival of glial cells in the peripheral nervous system, apoptotic cells were also counted. TUNEL-positive cells increased at 5 days after siRNA treatment. These results suggest that Sox2 promotes satellite glial cell survival through the MAPK pathway via ErbB receptors.
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Hirakata-City, Osaka 573-1010, Japan. Electronic address: koiket@hirakata.kmu.ac.jp.
authors.forename: Taro
authors.initials: T
authors.lastname: Koike
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Hirakata-City, Osaka 573-1010, Japan.
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Wakabayashi
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Hirakata-City, Osaka 573-1010, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Hirakata-City, Osaka 573-1010, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Hirahara
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Hirakata-City, Osaka 573-1010, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002470:Cell Survival; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D020893:Receptor, ErbB-3 / Q000235:genetics / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D020928:Mitogen-Activated Protein Kinases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; D055748:SOXB1 Transcription Factors; C527784:Sox2 protein, rat; C483804:Erbb2 protein, rat
keywords: Apoptosis; Culture; ErbB; Satellite glial cell; Sox2