title: Neovibsanin B inhibits human malignant brain tumor cell line proliferation and induces apoptosis.
abstract: The present study was designed to examine the effect of neovibsanin B on glioma cell viability, apoptosis and on the survival time in mice bearing tumor xenografts. The results demonstrated that neovibsanin B significantly reduced the cell viability of GL261-NS and GL261-AC cells in a dose-dependent manner. However the inhibition of proliferation was more significant in GL261-NS cells. The IC50 value of neovibsanin B against GL261-NS and GL261-AC cells is 5 and 25 nM, respectively. The inhibitory effect of neovibsanin B on cell growth was more effective than that of vincristine (VCR) (P < 0.05). We also observed a significant decrease in sphere-forming ability of GL261-NS cells on treatment with neovibsanin B. The number of colonies formed by GL261-NS cells on treatment with neovibsanin B, VCR and DMSO were 3.34 ± 1.02, 12.53 ± 3.46 and 61.34 ± 9.89% respectively after 7 days. The flow cytometry revealed a marked increase in apoptotic cell death of GL261-NS cells on treatment with neovibsanin B. The western blots showed a significant decrease in the level of activated caspase-3 on treatment with neovibsanin B after 24 h. In addition, neovibsanin B increased the median survival time of glioma-bearing mice (P < 0.05). Therefore, neovibsanin B effectively inhibits glioma cell viability by inducing apoptosis, and can be a potent therapeutic agent for the treatment of malignant glioma.
authors.affiliation: Institute of Neuroinformatics and Laboratory of Body and Mind, Dalian University of Technology Dalian 116023, China.
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Cui
authors.affiliation: Central lab, Zhongshan Hospital affiliated to Dalian University Dalian 116023, China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, Zhongshan Hospital affiliated to Dalian University Dalian 116023, China.
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Fan
authors.affiliation: Central lab, Zhongshan Hospital affiliated to Dalian University Dalian 116023, China.
authors.forename: Sheng-Fan
authors.initials: SF
authors.lastname: Li
authors.affiliation: Institute of Neuroinformatics and Laboratory of Body and Mind, Dalian University of Technology Dalian 116023, China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Deng
authors.affiliation: Central lab, Zhongshan Hospital affiliated to Dalian University Dalian 116023, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Central lab, Zhongshan Hospital affiliated to Dalian University Dalian 116023, China.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Central lab, Zhongshan Hospital affiliated to Dalian University Dalian 116023, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
keywords: Malignant glioma; apoptosis; neovibsanin B; sphere-forming ability; vincristine