title: Biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum exposed to enhanced UV-B radiation.
abstract: The article studied UV-B effects on biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum. The experiment about UV-B effects on biochemical traits in flowers included six levels of UV-B treatments (0 (UV0), 50 (UV50), 200 (UV200), 400 (UV400), 600 (UV600) and 800 (UV800) μWcm(-2)). UV400, UV600 and UV800 treatments significantly increased the contents of hydrogen peroxide, malondialdehyde and UV-B absorbing compounds, and the activity of phenylalanine ammonia lyase enzyme over the control. The contents of chlorogenic acid and flavone in flowers were significantly increased by UV-B treatments (except for UV50 and UV800). Two-dimensional gel electrophoresis was utilized to analyze proteomic changes in flowers with or without UV-B radiation. Results indicated that 43 protein spots (>1.5-fold difference in volume) were detected, including 19 spots with a decreasing trend and 24 spots with an increasing trend, and 19 differentially expressed protein spots were successfully indentified by MALDI-TOF MS. The indentified proteins were classified based on functions, the most of which were involved in photosynthesis, respiration, protein biosynthesis and degradation and defence. An overall assessment using biochemical and differential proteomic data revealed that UV-B radiation could affect biochemical reaction and promote secondary metabolism processes in postharvest flowers.
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China. Electronic address: yaoxiao301@126.com.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Yao
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Jian-Zhou
authors.initials: JZ
authors.lastname: Chu
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Ma
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Si
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Ji-Gang
authors.initials: JG
authors.lastname: Li
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Shi
authors.affiliation: The College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Chao-Nan
authors.initials: CN
authors.lastname: Liu
mesh_terms: D002726:Chlorogenic Acid / Q000378:metabolism; D031202:Chrysanthemum / Q000378:metabolism* / Q000528:radiation effects*; D047309:Flavones / Q000378:metabolism; D035264:Flowers / Q000378:metabolism* / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics; D014466:Ultraviolet Rays
chemical_list: D047309:Flavones; D010940:Plant Proteins; D002726:Chlorogenic Acid; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; C043562:flavone
keywords: Chlorogenic acid; Chrysanthemum; Flavone; Postharvest flowers; Proteomic analysis; UV-B radiation