title: PP010. Does aldosterone participate in placental angiogenesis via PLGF?
abstract: BACKGROUND
  Angiogenic signals are a vital signal of placental integrity. Aldosterone has recently been shown to enhance placental growth factor (PlGF) expression in the peripheral vasculature [1] and to promote trophoblast growth [2]. The plgf gene possesses a functional mineralocorticoid receptor responsive element in the promoter region.
  
  
  OBJECTIVE
  Thus, we hypothesized that aldosterone adapts placental angiogenesis to trophoblast growth by secreting PlGF.
  
  
  METHODS
  The human choriocarcinoma cell line BeWo and first and third trimester human primary trophoblasts cells were subjected to several syncytialization signals. Upon visual confirmation, the cultured cells were subjected to either control conditions, the known stimulator forskolin, and increasing amounts of aldosterone (10(-9) to 10(-6)M) with and without the competitive aldosterone receptor blocker spironolactone. After 6 and 24h of incubation, RNA and protein were extracted. PlGF transcripts were quantified by Taqman PCR normalized to several housekeeping genes. Protein expression was quantified by ELISA.
  
  
  RESULTS
  PlGF mRNA expression increased 3-fold with forskolin in BeWo cells. In this cell line, aldosterone could slightly stimulate PlGF production. In non-syncytialized primary human first trimester trophoblasts, aldosterone did not exert a specific effect. In contrast, the term primary human trophoblasts did respond with a 2.5-fold increase after incubation with aldosterone (10(-7)M) in the presence of forskolin to allow forming a syncytial layer. PlGF protein was already slightly upregulated following 6h of incubation with aldosterone.
  
  
  CONCLUSIONS
  We concluded that aldosterone does regulate PlGF expression in specified conditions during pregnancy. Inappropriately low aldosterone levels such as in preeclampsia might such not only compromise plasma volume and trophoblast growth but also placental vascularization and systemic PlGF availability. These observations merit further investigation.
authors.affiliation: Klinik für Nephrologie/Hypertonie, Universität Bern, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Eisele
authors.affiliation: Klinik für Nephrologie/Hypertonie, Universität Bern, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Gennari-Moser
authors.affiliation: Institute of Biochemistry and Molecular Medicine, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Albrecht
authors.affiliation: Division of Obstetrics, University Women's Hospital Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Baumann
authors.affiliation: Division of Obstetrics, University Women's Hospital Bern, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Surbek
authors.affiliation: Klinik für Nephrologie/Hypertonie, Inselspital, Universität Bern, Bern, Switzerland.
authors.forename: M G
authors.initials: MG
authors.lastname: Mohaupt