title: Polycaprolactone fiber meshes provide a 3D environment suitable for cultivation and differentiation of melanocytes from the outer root sheath of hair follicle.
abstract: Melanocytes differentiated from the stem cells of human hair follicle outer root sheath (ORS) have the potential for developing non-invasive treatments for skin disorders out of a minimal sample: of hair root. With a robust procedure for melanocyte cultivation from the ORS of human hair follicle at hand, this study focused on the identification of a suitable biocompatible, biodegradable carrier as the next step toward their clinical implementation. Polycaprolactone (PCL) is a known biocompatible material used for a number of medical devices. In this study, we have populated electrospun PCL fiber meshes with normal human epidermal melanocytes (NHEM) as well as with hair-follicle-derived human melanocytes from the outer root sheath (HUMORS) and tested their functionality in vitro. PCL fiber meshes evidently provided a niche for melanocytes and supported their melanotic properties. The cells were tested for gene expression of PAX3, PMEL, TYR and MITF, as well as for proliferation, expression of melanocyte marker proteins tyrosinase and glycoprotein 100 (gp100), L-DOPA-tautomerase enzymatic activity and melanin content. Reduced mitochondrial activity and PAX-3 gene expression indicated that the three-dimensional PCL scaffold supported differentiation rather than proliferation of melanocytes. The monitored melanotic features of both the NHEM and HUMORS cultivated on PCL scaffolds significantly exceeded those of two-dimensional adherent cultures.
authors.affiliation: Translational Centre for Regenerative Medicine, Leipzig University, Leipzig, Germany.
authors.forename: Vuk
authors.initials: V
authors.lastname: Savkovic
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Biology, Pharmacy, and Psychology, Leipzig University, Leipzig, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Flämig
authors.affiliation: Translational Centre for Regenerative Medicine, Leipzig University, Leipzig, Germany.
authors.forename: Marie
authors.initials: M
authors.lastname: Schneider
authors.affiliation: Translational Centre for Regenerative Medicine, Leipzig University, Leipzig, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Sülflow
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Biology, Pharmacy, and Psychology, Leipzig University, Leipzig, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Loth
authors.affiliation: Translational Centre for Regenerative Medicine, Leipzig University, Leipzig, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lohrenz
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Biology, Pharmacy, and Psychology, Leipzig University, Leipzig, Germany.
authors.forename: Michael Christian
authors.initials: MC
authors.lastname: Hacker
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Biology, Pharmacy, and Psychology, Leipzig University, Leipzig, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Schulz-Siegmund
authors.affiliation: Clinic and Policlinic for Dermatology, Venereology, and Allergology, Leipzig University Clinic, Leipzig, Germany.
authors.forename: Jan-Christoph
authors.initials: JC
authors.lastname: Simon
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D060833:Cellular Microenvironment / Q000187:drug effects*; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D008544:Melanocytes / Q000166:cytology* / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone
keywords: 3D culture; HUMORS; hair follicle; melanocyte; outer root sheath; polycaprolactone