title: Advanced Evaluation Strategies for Protein-Imprinted Polymer Nanobeads.
abstract: Molecularly imprinted polymers (MIPs) are synthetic affinity matrices capable of selective binding a specific target molecule. A strategy for competitive selectivity studies is developed providing information on the selective binding properties of MIPs in complex matrices. Batch rebinding experiments entail the target protease, two other proteins, and MIP nanobeads. The protease is inhibited by addition of pepstatin thus quenching the degradation of the other proteins. The proteins are analyzed via sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The relevance of competitive selectivity studies for the evaluation of MIP performance is further emphasized by comparison to single protein rebinding experiments.
authors.affiliation: Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Albert-Einstein-Allee 11, Ulm, 89081, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Pluhar
authors.affiliation: Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Albert-Einstein-Allee 11, Ulm, 89081, Germany. boris.mizaikoff@uni-ulm.de.
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
mesh_terms: D000818:Animals; D002417:Cattle; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine
keywords: competitive selectivity study; inhibition study; molecularly imprinted polymer; protease; protein-imprinted polymer