title: Development and validation of an in-house quantitative analysis method for cylindrospermopsin using hydrophilic interaction liquid chromatography-tandem mass spectrometry: Quantification demonstrated in 4 aquatic organisms.
abstract: The cyanobacterial toxin cylindrospermopsin (CYN) is of great concern in aquatic environments because of its incidence, multiple toxicity endpoints, and, therefore, the severity of health implications. It may bioaccumulate in aquatic food webs, resulting in high exposure concentrations to higher-order trophic levels, particularly humans. Because of accumulation at primary levels resulting from exposure to trace amounts of toxin, a sensitive analytical technique with proven aquatic applications is required. In the present study, a hydrophilic interaction liquid chromatographic-tandem mass spectrometric method with a lower limit of detection of 200 fg on column (signal-to-noise ratio = 3, n = 9) and a lower limit of quantification of 1 pg on column (signal-to-noise ratio = 11, n = 9) with demonstrated application in 4 aquatic organisms is described. The analytical method was optimized and validated with a linear range (r(2) = 0.999) from 0.1 ng mL(-1) to 100 ng mL(-1) CYN. Mean recovery of the extraction method was 98 ± 2%. Application of the method was demonstrated by quantifying CYN uptake in Scenedesmus subspicatus (green algae), Egeria densa (Brazilian waterweed), Daphnia magna (water flea), and Lumbriculus variegatus (blackworm) after 24 h of static exposure to 50 μg L(-1) CYN. Uptake ranged from 0.05% to 0.11% of the nominal CYN exposure amount. This constitutes a sensitive and reproducible method for extraction and quantification of unconjugated CYN with demonstrated application in 4 aquatic organisms, which can be used in further aquatic toxicological investigations.
authors.affiliation: Institute of Ecology, Technische Universität Berlin, Berlin, Germany.
authors.forename: Maranda
authors.initials: M
authors.lastname: Esterhuizen-Londt
authors.affiliation: Institute of Ecology, Technische Universität Berlin, Berlin, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Kühn
authors.affiliation: Institute of Ecology, Technische Universität Berlin, Berlin, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Pflugmacher
mesh_terms: D000470:Alkaloids; D059001:Aquatic Organisms / Q000737:chemistry / Q000187:drug effects*; D001427:Bacterial Toxins / Q000032:analysis*; D001938:Brazil; D002853:Chromatography, Liquid / Q000379:methods; D000087522:Cyanobacteria Toxins; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins / Q000032:analysis*; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D053719:Tandem Mass Spectrometry / Q000379:methods; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; C089595:cylindrospermopsin; D014498:Uracil
keywords: Aquatic food chain; Cylindrospermopsin; Liquid chromatography­-tandem mass spectrometry; Quantitative analysis