title: [Expression of a testis-specific gene 1700001022RIK in mice and its bioinformatic analysis].
abstract: OBJECTIVE
  To identify the expression characteristics of the 1700001022RIK (RIKEN cDNA 1700001022) gene in mice and explore its function by bioinformatic analysis.
  
  
  METHODS
  Using the expression profile of gene microarray, we detected the expression of a new testis-specific gene, 1700001022RIK, in mice. We analyzed its expression characteristics in the testis tissue and their changes in different developmental stages of the testis by RT-PCR, real-time RT-PCR, Western blot, and immunohistochemistry. We performed bioinformatic analysis using a bioinformatic software.
  
  
  RESULTS
  The 1700001022RIK gene was specifically expressed in the mouse testis in an age-dependent manner, most highly in the adult mice. The 1700001022RIK protein was mainly expressed in the spermatogonia, spermatocytes, and round spermatids of the adult mice. Bioinformatic analysis showed that the 1700001022RIK protein amino acid sequence had a high similarity in human and mice, which indicated that this gene was highly conserved in mammals.
  
  
  CONCLUSIONS
  1700001022RIK is a testis-specific gene mainly expressed in the spermatogonia, spermatocytes, and round spermatids of seminiferous tubules, which might be involved in the regulation of spermatogenesis.
authors.forename: Yu-chi
authors.initials: YC
authors.lastname: Li
authors.forename: Shou-ren
authors.initials: SR
authors.lastname: Lin
authors.forename: Man-ling
authors.initials: ML
authors.lastname: Luo
authors.forename: Huan
authors.initials: H
authors.lastname: Guo
authors.forename: Han-wei
authors.initials: HW
authors.lastname: Wu
authors.forename: Zhi-mao
authors.initials: ZM
authors.lastname: Jiang
authors.forename: Yao-ting
authors.initials: YT
authors.lastname: Gui
mesh_terms: D000367:Age Factors; D000818:Animals; D015153:Blotting, Western; D019295:Computational Biology; D018076:DNA, Complementary; D015870:Gene Expression; D023281:Genomics; D008297:Male; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics*; D012671:Seminiferous Tubules; D013087:Spermatids; D013090:Spermatocytes; D013091:Spermatogenesis / Q000235:genetics; D013093:Spermatogonia; D013737:Testis
chemical_list: D018076:DNA, Complementary; D018832:Molecular Chaperones