title: Insulin-like growth factor binding protein-3 affects osteogenic efficacy on dental implants in rat mandible.
abstract: Insulin like growth factor binding protein-3 (IGFBP-3) in bone cells and its utilization in dental implants have not been well studied. The aim of this study was to determine the osteogenic efficacy of chitosan gold nanoparticles (Ch-GNPs) conjugated with IGFBP-3 coated titanium (Ti) implants. Ch-GNPs were conjugated with IGFBP-3 plasmid DNA through a coacervation process. Conjugation was cast over Ti surfaces, and cells were seeded on coated surfaces. For in vitro analysis the expression of different proteins was analyzed by immunoblotting. For in vivo analysis, Ch-GNP/IGFBP-3 coated implants were installed in rat mandibles. Four weeks post-implantation, mandibles were examined by microcomputed tomography (μCT), immunohistochemistry, hematoxylin & eosin and tartrate resistance acid phosphatase staining. In vitro overexpressed Ch-GNP/IGFBP-3 coated Ti surfaces was associated with activation of extracellular signal related kinase (ERK), inhibition of the stress activated protein c-Jun N-terminal kinase (JNK) and enhanced bone morphogenetic protein (BMP)-2 and 7 compared to control. Further, in vivo, Ch-GNP/IGFBP-3 coated implants were associated with inhibition of implant induced osteoclastogenesis molecules, receptor activator of nuclear factor kappa-B ligand (RANKL) and enhanced expression of osteogenic molecules including BMP2/7 and osteopontin (OPN). The μCT analysis demonstrated that IGFBP-3 increased the volume of newly formed bone surrounding the implants compared to control (n=5; p<0.05). These results support the view that IGFBP-3 overexpression diminishes osteoclastogenesis and enhances osteogenesis of Ti implants, and can serve as a potent molecule for the development of good implantation.
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Govinda
authors.initials: G
authors.lastname: Bhattarai
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Dental Materials, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Division of Advanced Materials Engineering, Research Center for Advanced Materials, Development and Institute of Biodegradable Materials, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Il-Song
authors.initials: IS
authors.lastname: Park
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea. Electronic address: yihokn@chonbuk.ac.kr.
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Yi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015921:Dental Implants; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000502:physiology*; D008297:Male; D008334:Mandible / Q000601:surgery*; D051379:Mice; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015921:Dental Implants; D018972:Insulin-Like Growth Factor Binding Protein 3
keywords: Chitosan gold nanoparticles; Gene delivery; IGFBP-3; Implant; Osteogenesis; Surface modification