title: Aberrations and adaptive optics in super-resolution microscopy.
abstract: As one of the most powerful tools in the biological investigation of cellular structures and dynamic processes, fluorescence microscopy has undergone extraordinary developments in the past decades. The advent of super-resolution techniques has enabled fluorescence microscopy - or rather nanoscopy - to achieve nanoscale resolution in living specimens and unravelled the interior of cells with unprecedented detail. The methods employed in this expanding field of microscopy, however, are especially prone to the detrimental effects of optical aberrations. In this review, we discuss how super-resolution microscopy techniques based upon single-molecule switching, stimulated emission depletion and structured illumination each suffer from aberrations in different ways that are dependent upon intrinsic technical aspects. We discuss the use of adaptive optics as an effective means to overcome this problem.
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK martin.booth@eng.ox.ac.uk.
authors.forename: Martin
authors.initials: M
authors.lastname: Booth
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK.
authors.forename: Débora
authors.initials: D
authors.lastname: Andrade
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Burke
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK.
authors.forename: Brian
authors.initials: B
authors.lastname: Patton
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK.
authors.forename: Mantas
authors.initials: M
authors.lastname: Zurauskas
mesh_terms: D008029:Lighting / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*
keywords: aberrations; adaptive optics; single-molecule switching; stimulated emission depletion; structured illumination; super-resolution microscopy