title: PPP1R12A Copy Number Is Associated with Clinical Outcomes of Stage III CRC Receiving Oxaliplatin-Based Chemotherapy.
abstract: OBJECTIVE
  To investigate the correlation between PPP1R12A gene copy number and clinical outcomes of oxaliplatin-based regimen in stage III colorectal cancer (CRC).
  
  
  METHODS
  A total of 139 paraffin-embedded tissue samples of stage III CRC patients who received oxaliplatin-based treatment after radical surgery were recruited. Genomic DNA was extracted and purified from paraffin-embedded sections. Quantitative PCR methods were used to detect the relative copy number (RCN) of PPP1R12A.
  
  
  RESULTS
  Statistical analysis demonstrated that low PPP1R12A RCN was associated with poor RFS (HR = 2.186, 95% CI: 1.293-3.696; P = 0.003) and OS (HR = 2.782, 95% CI: 1.531-5.052; P < 0.001). Additionally, when patients were stratified according to subgroups of stage III and tumor location, poor RFS and OS were also observed in the low PPP1R12A RCN group with significance (RFS: IIIB HR = 2.870, P < 0.001; colon HR = 1.910, P = 0.037; OS: IIIB HR = 3.527, P < 0.001; IIIC HR = 2.662, P = 0.049; rectum HR = 4.229, P = 0.002).
  
  
  CONCLUSIONS
  Our findings suggest the copy number of PPP1R12A can independently predict recurrence and overall survival of stage III colorectal cancer patients receiving oxaliplatin-based chemotherapy.
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Ajian
authors.initials: A
authors.lastname: Li
authors.affiliation: Center for Translational Medicine, Yangpu Hospital Affiliated to Shanghai Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Huaguang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Kangsheng
authors.initials: K
authors.lastname: Peng
authors.affiliation: Department of Pathology, The Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Center for Translational Medicine, Yangpu Hospital Affiliated to Shanghai Tongji University School of Medicine, Shanghai 200090, China ; Department of General Surgery, Yangpu Hospital Affiliated to Shanghai Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Moubin
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Gastroenterology, The Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Zhanju
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Yangpu Hospital Affiliated to Shanghai Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D005260:Female; D018628:Gene Dosage; D006801:Humans; D008297:Male; D008875:Middle Aged; D043263:Myosin-Light-Chain Phosphatase / Q000235:genetics*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D009944:Organoplatinum Compounds / Q000627:therapeutic use*; D000077150:Oxaliplatin
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D043263:Myosin-Light-Chain Phosphatase; C490372:PPP1R12A protein, human