title: The C-terminal α-helix of YsxC is essential for its binding to 50S ribosome and rRNAs.
abstract: YsxC is an essential P-loop GTPase that interacts with the 50S subunit of the ribosome. The putative implication in ribosome binding of two basic clusters of YsxC, a conserved positively charged patch including R31, R116, H117 and K146 lying adjacent to the nucleotide-binding site, and the C-terminal alpha helix, was investigated. C-terminal truncation variants of YsxC were unable to bind to both ribosome and rRNAs, whereas mutations in the other cluster did not affect YsxC binding. Our results indicate that the basic C-terminal region of YsxC is required for its binding to the 50S ribosomal subunit.
authors.affiliation: CNRS, IBS, 6 rue Jules Horowitz, 38000 Grenoble, France; Université Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France; CNRS, IBS, F-38027 Grenoble, France; CEA, DSV, IBS, F-38027 Grenoble, France. Electronic address: catherine.wicker-planquart@ibs.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Wicker-Planquart
authors.affiliation: BMSSI, UMR 5086 CNRS/Université Claude Bernard Lyon I, France.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Ceres
authors.affiliation: CNRS, IBS, 6 rue Jules Horowitz, 38000 Grenoble, France; Université Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France; CNRS, IBS, F-38027 Grenoble, France; CEA, DSV, IBS, F-38027 Grenoble, France. Electronic address: jean-michel.jault@ibcp.fr.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Jault
mesh_terms: D019943:Amino Acid Substitution; D001120:Arginine / Q000737:chemistry; D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry; D006358:Hot Temperature; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055550:Protein Stability; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism*; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D054681:Ribosome Subunits, Large, Bacterial / Q000737:chemistry / Q000378:metabolism*; D054680:Ribosome Subunits, Small, Bacterial / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008024:Ligands; D050505:Mutant Proteins; D010446:Peptide Fragments; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011994:Recombinant Proteins; D006639:Histidine; D001120:Arginine; D020558:GTP Phosphohydrolases; D008239:Lysine
keywords: Bacillus subtilis; GTPase; Ribosome binding; YsxC