title: [Analysis of the Physiological Activities and Functions in Vitro of Apheresis Platelets during Storage].
abstract: OBJECTIVE
  To investigate the changes of physiological activities and functions in vitro of apheresis platelets during storage.
  
  
  METHODS
  17 units of apheresis platelets were randomly chosen and stored at 20 °C to 24 °C with agitation. Platelet counting (Plt), mean platelet volume (MPV), blood gases, pH value, glucose (Glu) concentration, lactate (LA) concentration, LDH concentration, thromboelastogram (TEG), hypotonic shock response (HSR), CD62p expression rate and anew expression rate were measured on days 0, 1, 3, 5 after platelet storage. Changes of physiological activities and functions in vitro were systematically evaluated by above-mentioned indexes.
  
  
  RESULTS
  During storage, Plt, MPV and HSR were not significantly changed; but pH value, blood gases, Glu, LA, LDH, HSR, expression rate of CD62p and anew expression rate were significant differenty. Among thromboelastogram indexes, R value increased obviously with prolongation of storage time; K value and αAngle were not significantly changed; MA was not significantly changed on day 1 and 3, but was slightly increase on day 5.
  
  
  CONCLUSIONS
  The physiological activities and functions in vitro of apheresis platelets are kept well during storage. For clinical transfusion of apheresis platelet during storage, clinical effect of transfusione is not influenced.
authors.affiliation: Department of Blood Transfusion, The Fourth Hospital Affiliated to China Medical University, Shenyang 110032, Liaoning Province, China.
authors.forename: Huan-Ming
authors.initials: HM
authors.lastname: Xu
authors.affiliation: Liaoning Provincial Blood Center, Shengyang 110044, Liaoning Province, China.
authors.forename: Dong-Yang
authors.initials: DY
authors.lastname: Zhao
authors.affiliation: Department of Blood Transfusion, The First Hospital Affiliated to China Medical Universoty, Shenyang 110001, Liaoning Province, China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Hao
authors.affiliation: Department of Utrasonics, The First Hospital Affiliated to China Medical Universoty, Shenyang 110001, Liaoning Province, China.
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Gao
authors.affiliation: Department of Blood Transfusion, The Fourth Hospital Affiliated to China Medical University, Shenyang 110032, Liaoning Province, China. E-mail: 317780708@qq.com.
authors.forename: Ji-Xiang
authors.initials: JX
authors.lastname: Cui
mesh_terms: D001792:Blood Platelets; D001793:Blood Preservation; D006801:Humans; D066298:In Vitro Techniques; D010976:Platelet Count; D010983:Plateletpheresis; D013916:Thrombelastography