title: Transcription factors GATA4 and TBX5 promote cardiomyogenic differentiation of rat bone marrow mesenchymal stromal cells.
abstract: Bone marrow mesenchymal stromal cells (BMSCs) have potential applications in cell and gene therapies for cardiac disease. The cardiac-specific transcription factors GATA-binding protein 4 (GATA4) and T-Box protein 5 (TBX5) are considered to be pivotal in cardiogenesis. The aim of this study was to investigate the effects of GATA4 and TBX5 on cardiomyogenic differentiation of BMSCs. The BMSCs were initially isolated and identified. Vectors harboring cardiac transcription factor genes GATA4 and TBX5 or empty vectors were transferred into BMSCs. Cardiomyogenic cells differentiated from BMSCs were identified by expression of cardiac-specific markers including cardiac troponin T, connexin 43, β-myosin heavy chain, and myosin light chain-2 using immunocytochemical staining, western blotting, and quantitative real-time PCR. The ultrastructures of the differentiated cells were examined by transmission electron microscopy, which were similar to those of fetal cardiomyocytes. The differentiated cells exhibited L-type calcium current activities reflective of the electrophysiological characteristics of cardiomyocytes. These findings indicate that exogenous expression of cardiac-specific transcription factors GATA4 and TBX5 enhance cardiomyogenic differentiation of BMSCs.
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China. zhanglei@hebmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Su-Xia
authors.initials: SX
authors.lastname: Shao
authors.affiliation: Department of Histology and Embryology, Hebei North University, Zhangjiakou City, Hebei Province, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Cui
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhang
authors.affiliation: Department of Neurology, the 1st Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Xiang-Zeng
authors.initials: XZ
authors.lastname: Kong
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D055724:Electrophysiological Phenomena; D050991:GATA4 Transcription Factor / Q000235:genetics* / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*; D010011:Osteocytes / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000502:physiology*; D014162:Transfection
chemical_list: D050991:GATA4 Transcription Factor; C084365:Gata4 protein, rat; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5