title: Molecular cloning, sequence characteristics, and tissue expression analysis of ECE1 gene in Tibetan pig.
abstract: Low air pressure and low oxygen partial pressure at high altitude seriously affect the survival and development of human beings and animals. ECE1 is a recently discovered gene that is involved in anti-hypoxia, but the full-length cDNA sequence has not been obtained. For a better understanding of the structure and function of the ECE1 gene and to study its effect in Tibetan pig, the cDNA of the ECE1 gene from the muscle of Tibetan pig was cloned, sequenced and characterized. The ECE1 full-length cDNA sequence consists of 2262 bp coding sequence (CDS) that encodes 753 amino acids with a molecular mass of 85,449 kD, 2 bp 5'UTR and 1507 bp 3'UTR. In addition, the phylogenetic tree analysis revealed that the Tibetan pig ECE1 has a closer genetic relationship and evolution distance with the land mammals ECE1. Furthermore, analysis by qPCR showed that the ECE1 transcript is constitutively expressed in the 10 tissues tested: the liver, subcutaneous fat, kidney, muscle, stomach, heart, brain, spleen, pancreas, and lung. These results serve as a foundation for further insight into the Tibetan pig ECE1 gene.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, PR China.
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Wang
authors.affiliation: Station of Animal Science and Veterinary, Gongbo'gyamda County, Linzhi, Tibet 860200, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, PR China.
authors.forename: Chuan-Hao
authors.initials: CH
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, PR China.
authors.forename: Hai-Peng
authors.initials: HP
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, PR China. Electronic address: yqzeng@sdau.edu.cn.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Zeng
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, PR China. Electronic address: wanghui2328@sdau.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000531:Altitude; D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D000072396:Endothelin-Converting Enzymes; D020869:Gene Expression Profiling; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics* / Q000378:metabolism; D018609:Tibet; D014018:Tissue Distribution
chemical_list: D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes
keywords: Anti-hypoxia; ECE1; Expression; Sequence analysis; Tibetan pig; cDNA clone