title: THE EFFECT OF DIHYDROARTEMISININ ON THE PROLIFERATION, METASTASIS AND APOPTOSIS OF HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM.
abstract: This study aims to research the effect of dihydroartemisinin on the proliferation, metastasis and apoptosis in human osteosarcoma cells 143B and the underlying mechanism. This study designed five groups for experiment and control, using dimethylsulfoxide (DMSO), and docosahexaenoic acid (DHA) at concentrations of 15, 25, 35 μmol.L-1 respectively. Experiments including methyl thiazolyl tetrazolium (MTT) assay, clone formation assay, Hoechst 33258 staining assay, luciferase reporter plasmid assay, Western blot and scratch test were carried out. In addition, SPSS 18.0 software from IBM was used for statistical analysis and all the data obtained from the experiments were expressed as mean ± SD, and variance was used to compare the difference between the groups. DHA is proved to be able to inhibit the proliferation and metastasis of osteosarcoma cells, as well as leaving a positive effect on apoptosis in the cytomorphosis. It achieves regulation over the human osteosarcoma cells by keeping the expression of related protein under control.
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: D W
authors.initials: DW
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Yu
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: E
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Yao
authors.affiliation: Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Bi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D037621:Artemisinins / Q000494:pharmacology*; D006690:Bisbenzimidazole; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D017930:Genes, Reporter; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D012516:Osteosarcoma / Q000473:pathology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014410:Tumor Stem Cell Assay
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D037621:Artemisinins; D004396:Coloring Agents; D009363:Neoplasm Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D004281:Docosahexaenoic Acids; C039060:artenimol; C022616:thiazolyl blue; D006690:Bisbenzimidazole; D004121:Dimethyl Sulfoxide