title: A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice.
abstract: Currently, the metabolic syndrome (MS) is occurring at growing rates worldwide, raising extensive concerns on the mechanisms and therapeutic interventions for this disorder. Herein, we described a novel method of establishing MS model in rodents. Male Institute of Cancer Research (ICR) mice were fed with high-fat-high-fructose (HFHF) diet or normal chow (NC) respectively for 12 weeks. Metabolic phenotypes were assessed by glucose tolerance test, insulin tolerance test and hyperinsulinemic-euglycemic clamp. Blood pressure was measured by a tail-cuff system. At the end of the experiment, mice were sacrificed, and blood and tissues were harvested for subsequent analysis. Serum insulin levels were measured by ELISA, and lipid profiles were determined biochemically. The HFHF diet-fed ICR mice exhibited obvious characteristics of the components of MS, including obvious obesity, severe insulin resistance, hyperinsulinemia, dislipidemia, significant hypertension and hyperuricemia. Our data suggest that HFHF diet-fed ICR mice may be a robust and efficient animal model that could well mimic the basic pathogenesis of human MS.
authors.affiliation: Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhuhua
authors.forename: Wang
authors.initials: W
authors.lastname: Zhiquan
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhen
authors.forename: Niu
authors.initials: N
authors.lastname: Yixin
authors.forename: Zhang
authors.initials: Z
authors.lastname: Weiwei
authors.forename: Li
authors.initials: L
authors.lastname: Xiaoyong
authors.forename: Liu
authors.initials: L
authors.lastname: Yueming
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hongmei
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Su
authors.initials: S
authors.lastname: Qing
mesh_terms: D000821:Animal Feed; D000818:Animals; D015415:Biomarkers / Q000097:blood; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D050171:Dyslipidemias; D005632:Fructose / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D006946:Hyperinsulinism; D006973:Hypertension; D033461:Hyperuricemia; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000175:diagnosis / Q000209:etiology; D008813:Mice, Inbred ICR; D010641:Phenotype; D013997:Time Factors
chemical_list: D015415:Biomarkers; D007328:Insulin; D008055:Lipids; D005632:Fructose