title: UriSed as an Alternative to Phase-Contrast Microscopy in the Differentiation between Glomerular and Non-Glomerular Hematuria.
abstract: BACKGROUND
  Differentiation between glomerular and non-glomerular hematuria by observation of the erythrocyte morphology using phase-contrast is a time-consuming and labor-intensive procedure that requires skilled personnel. This paper has the purpose to evaluate the performance of UriSed (also called sediMAX in some countries) as an alternative to the phase-contrast microscopic analysis of erythrocyte morphology.
  
  
  METHODS
  312 urine samples with hematuria were analyzed by UriSed and by phase-contrast microscopy. Based on the presence of codocytes and/or acanthocytes, samples were classified as non-glomerular and glomerular. Kappa correlation was used to assess the agreement between both methods.
  
  
  RESULTS
  Our data showed excellent agreement between erythrocyte morphology analyzed by both methods (r = 0.974, kappa = 0.9484, p < 0.001) with only 8 samples presenting discordant results.
  
  
  CONCLUSIONS
  UriSed proved to be a precise and accurate alternative to the gold standard phase-contrast microscopy.
authors.forename: Paula V
authors.initials: PV
authors.lastname: Bottini
authors.forename: Bruna D
authors.initials: BD
authors.lastname: Andreguetto
authors.forename: Kelly
authors.initials: K
authors.lastname: Krempser
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: Lauand
authors.forename: Célia R
authors.initials: CR
authors.lastname: Garlipp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000473:pathology*; D005260:Female; D006417:Hematuria / Q000473:pathology*; D006801:Humans; D007223:Infant; D007674:Kidney Diseases / Q000473:pathology*; D008297:Male; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D055815:Young Adult