title: [Salinomycin Enhances the Apoptosis of T-cell Acute Lymphoblastic Leukemia Cell Line Jurkat Cells Induced by Vincristine].
abstract: OBJECTIVE
  This study was aimed to investigate the effect of salinomycin combined with vincristine on the proliferation and apoptosis of Jurkat cells and its possible mechanisms.
  
  
  METHODS
  The proliferation of Jurkat cells was examined by CKK-8 assay. Flow cytometry was used to assess cellular apoptosis. Levels of BCL-2, caspase-3, and caspase- 8 were measured by Western blot.
  
  
  RESULTS
  The salinomycin or vincristine, either alone or in combination, inhibited the proliferation of Jurkat cells in a dose-dependent manner. Salinomycin combined with vincristine produced more obveous inhibition of cell proliferation than either compound used alone (P<0.05). Western blot analysis showed that the combined use of Sal and VCR reduced the expression of BCL-2 protein, and increased expression of caspase 3 and caspase 8 protein, more significantly. Furthermore, combination of Sal and VCR synergistally promoted apoptosis of the Jurkat cells (P<0.05).
  
  
  CONCLUSIONS
  The combination of salinomycin and vincristine synergistically inhibits proliferation and promotes apoptosis of T-cell acute lymphoblastic leukemia Jurkat cells.
authors.affiliation: Medical Center, Tsinghua University, Beijing 100084, China.|Department of Hematology and Oncology, Tsinghua University First Hospital, Beijing 100016, China. E-mail: hnlpp@163.com.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Liu
authors.affiliation: Institute of Hematology, Jinan University Medical College, Guangzhou 510632, Guangdong Province, China.
authors.forename: Jin-Can
authors.initials: JC
authors.lastname: Zhu
authors.affiliation: Institute of Hematology, Jinan University Medical College, Guangzhou 510632, Guangdong Province, China.
authors.forename: Ge-Xiu
authors.initials: GX
authors.lastname: Liu
authors.affiliation: Medical Center, Tsinghua University, Beijing 100084, China.
authors.forename: Chao-Xiang
authors.initials: CX
authors.lastname: Shui
authors.affiliation: Medical Center, Tsinghua University, Beijing 100084, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D053181:Caspase 8; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; D011714:Pyrans; D014750:Vincristine; D000095662:Polyether Polyketides
chemical_list: D053148:Caspase 3; D053181:Caspase 8; D011714:Pyrans; D014750:Vincristine; C010327:salinomycin; D000095662:Polyether Polyketides; C505407:CASP3 protein, human; C505530:CASP8 protein, human