title: Shape Transformation of Light-Responsive Pyrene-Containing Micelles and Their Influence on Cytoviability.
abstract: The amphiphilic pyrene-containing random copolymers with light-responsive pyrene ester bonds were synthesized by copolymerizing 1-pyrenemethyl acrylate (PA) and N,N-dimethylacrylamide (DMA). The P(DMA-co-PA) copolymers formed spherical micelles in water, which were transformed into nanorods as a result of cleavage of the pyrene ester bonds under UV irradiation. In vitro culture with A549 cells and Raw cells showed that compared to the nonphotodegradable ones, the photodegradable P(DMA-co-PA) micelles caused significantly higher cytotoxicity under the same UV irradiation. The intracellular reactive oxygen species (ROS) level had a positive correlation with the cytotoxicity regardless of the cell types. The nonphotodegradable pyrene-containing micelles produced a lower level of ROS under UV irradiation. However, the photodecomposable P(DMA-co-PA) micelles produced a significant higher level of ROS under the same trigger of UV irradiation, which caused the shape transformation of micelles to nanorods and higher cytotoxicity simultaneously.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D008027:Light; D008823:Micelles; D043942:Nanotubes / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D011721:Pyrenes / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry; D013696:Temperature; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D011721:Pyrenes; D017382:Reactive Oxygen Species; D014867:Water; C030984:pyrene; C099046:N,N-dimethylacrylamide