title: Morphometric analysis and developmental comparison of embryos from carriers with balanced chromosomal rearrangements in preimplantation genetic diagnosis cycles.
abstract: The morphological parameters of embryos from 22 carriers with balanced chromosomal rearrangements (CRs) were quantified and evaluated to determine their possible link to chromosomal composition. The morphometric characteristics of 168 embryos diagnosed by fluorescence in situ hybridisation were measured using an imaging tool and then analysed retrospectively. The mean zygotic diameter of normal-balanced embryos was significantly smaller compared with that of abnormal embryos (P=0.015). In addition, the reduction in total cytoplasmic volume for Day-3 embryos was significantly lower in normal or balanced embryos than in abnormal embryos (P=0.027). Moreover, the pronuclear volumes of embryos that failed to reach the blastocyst stage were significantly smaller compared with those of blastocysts (P=0.016). These findings indicate that morphometric characteristics are correlated with developmental outcomes as well as with chromosomal composition in embryos from balanced CR carriers. However, an effective indicator of developmental outcomes may not accurately reflect chromosomal composition. Combining morphometric and traditional qualitative assessment may increase the precision and standardisation of embryo evaluation as well as contributing to improved efficiency of preimplantation genetic diagnosis by selecting embryos with high developmental potential and preferentially testing embryos predicted to have a low risk of chromosomal imbalance.
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Baoheng
authors.initials: B
authors.lastname: Gui
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Yao
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Huang
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Libin
authors.initials: L
authors.lastname: Mei
authors.affiliation: The Reproductive Medical Center of Xiangya Hospital, Central South University, No. 87, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Reproductive Medical Center of Xiangya Hospital, Central South University, No. 87, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Donge
authors.initials: D
authors.lastname: Liu
authors.affiliation: The Reproductive Medical Center of Xiangya Hospital, Central South University, No. 87, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Nenghui
authors.initials: N
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Desheng
authors.initials: D
authors.lastname: Liang
authors.affiliation: The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
authors.forename: Lingqian
authors.initials: L
authors.lastname: Wu
mesh_terms: D000328:Adult; D001755:Blastocyst; D005260:Female; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D014178:Translocation, Genetic