title: [Roles of histone acetyltransferase and histone deacetylase in the pathogenesis of bronchial asthma].
abstract: OBJECTIVE
  To evaluate the roles of various cytokines, histone acetyltransferase (HAT) and histone deacetylase (HDAC) in the pathogenesis of bronchial asthma.
  
  
  METHODS
  BALB/C mice were randomly assigned to control, untreated asthma, hormone treatment and TSA treatment groups. Bronchial asthma was induced by intraperitoneal injections and atomization inhalation of ovalbumin (OVA) in the asthma, hormone treatment and trichostatin (TSA) treatment groups. The mice in the hormone treatment and TSA treatment groups were administered with dexamethasone 1.0 mg/kg and TSA 1.0 mg/kg respectively by an intraperitoneal injection 30 minutes before challenge of asthma. At 24 hours after the last challenge, IL-4, IL-8 and IFN- levels in serum were measured using ELISA, and activities of HAT and HDAC in peripheral blood mononuclear cells (PBMC) were determined by the enzyme linked immunofluorescence assay.
  
  
  RESULTS
  The serum levels of IL-4 and IL-8 in the untreated asthma group were higher than in the control, hormone treatment and TSA treatment groups (P<0.05). There was no difference in the serum levels of IL-4 and IL-8 among the control, hormone treatment and TSA treatment groups (P>0.05). The activity of HDAC in the untreated asthma group was lower than in the control, hormone treatment and TSA treatment groups (P<0.05). Hormone treatment significantly decreased the activity of HAT compared with the untreated asthma group (P<0.05). There was no difference in the activities of HAT and HDAC among the control, hormone treatment and TSA treatment groups (P>0.05).
  
  
  CONCLUSIONS
  The decreased activity of HDAC leads to an increased secretion of inflammatory factors and thus induces asthma.
authors.affiliation: Department of Pediatric Respiratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214000, China. ggzhou2003@yahoo.com.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Pan
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001249:Asthma / Q000201:enzymology / Q000209:etiology* / Q000276:immunology; D016207:Cytokines / Q000097:blood; D051548:Histone Acetyltransferases / Q000502:physiology*; D006655:Histone Deacetylases / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases