title: Enhanced Bone Tissue Regeneration by Porous Gelatin Composites Loaded with the Chinese Herbal Decoction Danggui Buxue Tang.
abstract: Danggui Buxue Tang (DBT) is a traditional Chinese herbal decoction containing Radix Astragali and Radix Angelicae sinensis. Pharmacological results indicate that DBT can stimulate bone cell proliferation and differentiation. The aim of the study was to investigate the efficacy of adding DBT to bone substitutes on bone regeneration following bone injury. DBT was incorporated into porous composites (GGT) made from genipin-crosslinked gelatin and β-triclacium phosphates as bone substitutes (GGTDBT). The biological response of mouse calvarial bone to these composites was evaluated by in vivo imaging systems (IVIS), micro-computed tomography (micro-CT), and histology analysis. IVIS images revealed a stronger fluorescent signal in GGTDBT-treated defect than in GGT-treated defect at 8 weeks after implantation. Micro-CT analysis demonstrated that the level of repair from week 4 to 8 increased from 42.1% to 71.2% at the sites treated with GGTDBT, while that increased from 33.2% to 54.1% at GGT-treated sites. These findings suggest that the GGTDBT stimulates the innate regenerative capacity of bone, supporting their use in bone tissue regeneration.
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Wang
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Integrated Chinese and Western Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Shi-Yuan
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Shung-Te
authors.initials: ST
authors.lastname: Kao
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Medicine, Taichung Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
authors.forename: Yuan-Tsung
authors.initials: YT
authors.lastname: Fu
authors.affiliation: Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Tzong-Fu
authors.initials: TF
authors.lastname: Kuo
authors.affiliation: Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.
authors.forename: Kuo-Yu
authors.initials: KY
authors.lastname: Chen
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan; Department of Biomedical Informatics, Asia University, Taichung, Taiwan.
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
mesh_terms: D029971:Angelica sinensis / Q000378:metabolism; D000818:Animals; D027883:Astragalus Plant / Q000378:metabolism; D027885:Astragalus propinquus; D001672:Biocompatible Materials / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D050723:Fractures, Bone / Q000628:therapy*; D005780:Gelatin / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D018517:Plant Roots / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D004365:Drugs, Chinese Herbal; C477279:danggui buxue decoction; D005780:Gelatin; C027492:Huang Qi; C044613:angelicae sinensis extract