title: Hydrogen Sulfide Ameliorates Early Brain Injury Following Subarachnoid Hemorrhage in Rats.
abstract: Increasing studies have demonstrated the neuroprotective effect of hydrogen sulfide (H2S) in central nervous system (CNS) diseases. However, the potential application value of H2S in the therapy of subarachnoid hemorrhage (SAH) is still not well known. This study was to investigate the potential effect of H2S on early brain injury (EBI) induced by SAH and explore the underlying mechanisms. The role of sodium hydrosulfide (NaHS), a donor of H2S, in SAH-induced EBI, was investigated in both in vivo and in vitro. A prechiasmatic cistern single injection model was used to produce experimental SAH in vivo. In vitro, cultured primary rat cortical neurons and human umbilical vein endothelial cells (HUVECs) were exposed to OxyHb at concentration of 10 μM to mimic SAH. Endogenous production of H2S in the brain was significantly inhibited by SAH. The protein levels of the predominant H2S-generating enzymes in the brain, including cystathionineb-synthase (CBS) and 3-mercaptopyruvate sulfur transferase (3MST), were also correspondingly reduced by SAH, while treatment with NaHS restored H2S production and the expressions of CBS and 3MST. More importantly, NaHS treatment could significantly attenuate EBI (including brain edema, blood-brain barrier disruption, brain cell apoptosis, inflammatory response, and cerebral vasospasm) after SAH. In vitro, H2S protects neurons and endothelial function by functioning as an antioxidant and antiapoptotic mediator. Our results suggest that NaSH as an exogenous H2S donor could significantly reduce EBI induced by SAH.
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Duan
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Baoqi
authors.initials: B
authors.lastname: Dang
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Anju
authors.initials: A
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China. sz_neurosurgery@163.com.
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China. nju_neurosurgery@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000097:blood / Q000188:drug therapy* / Q000209:etiology* / Q000473:pathology; D020169:Caspases / Q000378:metabolism; D002536:Cerebral Arteries / Q000187:drug effects / Q000473:pathology; D003541:Cystathionine beta-Synthase / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use*; D007249:Inflammation / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010108:Oxyhemoglobins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013345:Subarachnoid Hemorrhage / Q000097:blood / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D013466:Sulfurtransferases / Q000378:metabolism; D013702:Temporal Lobe / Q000473:pathology; D020301:Vasospasm, Intracranial / Q000097:blood / Q000150:complications / Q000188:drug therapy / Q000473:pathology
chemical_list: D010108:Oxyhemoglobins; D017382:Reactive Oxygen Species; D013466:Sulfurtransferases; C021550:3-mercaptopyruvate sulphurtransferase; D020169:Caspases; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide
keywords: Early brain injury; Hydrogen sulfide; Inflammatory response; Oxidative stress; Subarachnoid hemorrhage