title: Stable Carbon Isotope Fractionation During 1,2-Dichloropropane-to-Propene Transformation by an Enrichment Culture Containing Dehalogenimonas Strains and a dcpA Gene.
abstract: A stable enrichment culture derived from Besòs river estuary sediments stoichiometrically dechlorinated 1,2-dichloropropane (1,2-DCP) to propene. Sequential transfers in defined anaerobic medium with the inhibitor bromoethanesulfonate produced a sediment-free culture dechlorinating 1,2-DCP in the absence of methanogenesis. Application of previously published genus-specific primers targeting 16S rRNA gene sequences revealed the presence of a Dehalogenimonas strain, and no amplification was obtained with Dehalococcoides-specific primers. The partial sequence of the 16S rRNA amplicon was 100% identical with Dehalogenimonas alkenigignens strain IP3-3. Also, dcpA, a gene described to encode a corrinoid-containing 1,2-DCP reductive dehalogenase was detected. Resistance of the dehalogenating activity to vancomycin, exclusive conversion of vicinally chlorinated alkanes, and tolerance to short-term oxygen exposure is consistent with the hypothesis that a Dehalogenimonas strain is responsible for 1,2-DCP conversion in the culture. Quantitative PCR showed a positive correlation between the number of Dehalogenimonas 16S rRNA genes copies in the culture and consumption of 1,2-DCP. Compound specific isotope analysis revealed that the Dehalogenimonas-catalyzed carbon isotopic fractionation (εC(bulk)) of the 1,2-DCP-to-propene reaction was -15.0 ± 0.7‰ under both methanogenic and nonmethanogenic conditions. This study demonstrates that carbon isotope fractionation is a valuable approach for monitoring in situ 1,2-DCP reductive dechlorination by Dehalogenimonas strains.
authors.affiliation: †Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), Carrer de les Sitges s/n, 08193 Bellaterra, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Martín-González
authors.affiliation: †Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), Carrer de les Sitges s/n, 08193 Bellaterra, Spain.
authors.forename: S Hatijah
authors.initials: SH
authors.lastname: Mortan
authors.affiliation: ‡Departament de Crystal-lografia, Mineralogia i Dipòsits Minerals, Facultat de Geologia, Universitat de Barcelona (UB), Martí Franquès s/n, 08028. Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Rosell
authors.affiliation: §Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Parladé
authors.affiliation: §Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Alonso
authors.affiliation: §Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Gaju
authors.affiliation: ∥Institut de Química Avançada de Catalunya (IQAC) CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Caminal
authors.affiliation: ⊥Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, Leipzig, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Adrian
authors.affiliation: †Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), Carrer de les Sitges s/n, 08193 Bellaterra, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Marco-Urrea
mesh_terms: D000473:Alkanes / Q000737:chemistry / Q000378:metabolism; D000475:Alkenes / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis* / Q000737:chemistry; D005591:Chemical Fractionation; D041862:Chloroflexi / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology; D054879:Halogenation; D008969:Molecular Sequence Data; D011407:Propane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013030:Spain; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000473:Alkanes; D000475:Alkenes; D002247:Carbon Isotopes; D012336:RNA, Ribosomal, 16S; D014640:Vancomycin; C013658:propylene; C004765:propylene dichloride; D011407:Propane