title: Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture.
abstract: Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.
authors.affiliation: Department of Biology, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: INSERM, U941, Paris, France.
authors.forename: Ana
authors.initials: A
authors.lastname: Godinho-Santos
authors.affiliation: INSERM, U941, Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rato
authors.affiliation: 1] INSERM, U941, Paris, France [2] Univ Paris Diderot, Sorbonne Paris Cité, IUH, Paris, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Vanwalscappel
authors.affiliation: 1] INSERM, U941, Paris, France [2] Univ Paris Diderot, Sorbonne Paris Cité, IUH, Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Clavel
authors.affiliation: 1] Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo, Japan [2] Graduate School of Information Science and Technology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Aihara
authors.affiliation: 1] Department of Biology, Kyushu University, Fukuoka 812-8581, Japan [2] PRESTO, JST, Kawaguchi, Saitama 3320012, Japan [3] CREST, JST, Kawaguchi, Saitama 3320012, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Iwami
authors.affiliation: 1] INSERM, U941, Paris, France [2] Univ Paris Diderot, Sorbonne Paris Cité, IUH, Paris, France.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Mammano
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques; D057809:HEK293 Cells; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D008954:Models, Biological; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I