title: Antiallergic effects of peiminine through the regulation of inflammatory mediators in HMC-1 cells.
abstract: Peiminine is the main biologically active component derived from Fritillaria ussuriensis. Peiminine was investigated in various pulmonary diseases, but its antiallergic effect and the related mechanism have not been reported yet. The present study aimed to evaluate the effect of peiminine on mast cell-mediated allergic inflammation in HMC-1 cells. The pro-inflammatory cytokine production was measured using ELISA, reverse transcription-polymerase chain reaction and nuclear factor-kappaB (NF-κB), mitogen-activated protein kinases (MAPKs) pathway activation, as determined by Western blot analysis. Peiminine inhibits the production of the pro-inflammatory cytokine, such as interleukin (IL)-6, IL-8, tumor necrosis factor-alpha (TNF-α) and IL-1beta (IL-1β). It was shown to have inhibitory effects on MAPKs phosphorylation and NF-B expression in human mast cells (HMC)-1 using Western blot. HMC-1 cells were observed for confirmation of histamine release. Passive cutaneous anaphylaxis (PCA) reactions were evaluated using an animal model and peiminine demonstrated inhibitory effects on IgE-dependent anaphylaxis. These results suggest that peiminine has regulatory potential for allergic inflammatory reactions mediated by HMC-1 cells.
authors.affiliation: Department of Anatomy, College of Korean Medicine, Kyung Hee University Seoul , Republic of Korea.
authors.forename: Bina
authors.initials: B
authors.lastname: Lee
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Min
authors.forename: Da-Won
authors.initials: DW
authors.lastname: Jeong
authors.forename: Jae-Yun
authors.initials: JY
authors.lastname: Jun
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Cho
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Sohn
authors.forename: Hyuk-Sang
authors.initials: HS
authors.lastname: Jung
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002595:Cevanes / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006636:Histamine Release / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018926:Anti-Allergic Agents; D002595:Cevanes; D016207:Cytokines; D007073:Immunoglobulin E; C047331:peiminine
keywords: Cytokine; MAP kinases; inflammation; nuclear factor-kappaB; peiminine