title: Dual-Modality Noninvasive Mapping of Sentinel Lymph Node by Photoacoustic and Near-Infrared Fluorescent Imaging Using Dye-Loaded Mesoporous Silica Nanoparticles.
abstract: The imaging of sentinel lymph nodes (SLNs), the first defense against primary tumor metastasis, has been considered as an important strategy for noninvasive tracking tumor metastasis in clinics. In this study, we developed an imaging contrast system based on fluorescent dye-loaded mesoporous silica nanoparticles (MSNPs) that integrate near-infrared (NIR) fluorescent and photoacoustic (PA) imaging modalities for efficient SLN mapping. By balancing the ratio of dye and nanoparticles for simultaneous optimization of dual-modality imaging (NIR and PA), the dye encapsulated MSNP platform was set up to generate both a moderate NIR emission and PA signals simultaneously. Moreover, the underlying mechanisms of the relevance between optical and PA properties were discovered. Subsequently, dual-modality imaging was achieved to visualize tumor draining SLNs up to 2 weeks in a 4T1 tumor metastatic model. Obvious differences in uptake rate and contrast between metastatic and normal SLNs were observed both in vivo and ex vivo. Based on all these imaging data, it was demonstrated that the dye-loaded MSNPs allow detection of regional lymph nodes in vivo with time-domain NIR fluorescent and PA imaging methods efficiently.
authors.affiliation: School of Pharmaceutical Sciences, Central South University , Changsha, 410013, P. R. China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Radiology, the Third Xiangya Hospital, Central South University , Changsha, 410013, P. R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Rong
authors.affiliation: School of Pharmaceutical Sciences, Central South University , Changsha, 410013, P. R. China.
authors.forename: Lun
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Pharmaceutical Sciences, Central South University , Changsha, 410013, P. R. China.
authors.forename: Xintong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Radiology, the Third Xiangya Hospital, Central South University , Changsha, 410013, P. R. China.
authors.forename: Cejun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Radiology, the Third Xiangya Hospital, Central South University , Changsha, 410013, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Radiology, the Third Xiangya Hospital, Central South University , Changsha, 410013, P. R. China.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Lab Powder Met, Central South University , Changsha, 410083, P. R. China.
authors.forename: Kechao
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Radiology, the Third Xiangya Hospital, Central South University , Changsha, 410013, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Central South University , Changsha, 410013, P. R. China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology* / Q000601:surgery; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D008198:Lymph Nodes / Q000473:pathology* / Q000601:surgery; D051379:Mice; D008807:Mice, Inbred BALB C; D064847:Multimodal Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D061088:Photoacoustic Techniques / Q000379:methods*; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D005456:Fluorescent Dyes; D012822:Silicon Dioxide
keywords: mesoporous silica nanoparticles (MSNPs); multimodality imaging; near-infrared fluorescence (NIR) imaging; photoacoustic (PA) imaging; sentinel lymph nodes (SLNs)