title: Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation.
abstract: In this study, we further investigated a previously developed aptamer targeting ROS 17/2.8 (rat osteosarcoma) cells. We found that this C6-8 aptamer specifically binds to heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 and that it specifically labeled multiple tumor-cell lines as effectively as hnRNP A2/B1 monoclonal antibodies. When conjugated with fluorescent carbon nanodots (CDots) it could freely enter multiple living tumor cell lines (HepG2, MCF-7, H1299, and HeLa), whose growth it inhibited by targeting hnRNP A2/B1. Similar inhibitory effects were observed when the GFP-HepG2 hepatocarcinoma cells treated with C6-8-conjugated CDots were implanted in nude mice. Our work provides a new aptamer for targeting/labeling multiple tumor cell types, and its nanoparticle conjugates bring further advantages that increase its potential for use in cancer diagnosis and therapy.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory for Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, No. 27 Taiping Road, Haidian District, 100850, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Aixue
authors.initials: A
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, No. 27 Taiping Road, Haidian District, 100850, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ding
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Su
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Ge
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Leqiao
authors.initials: L
authors.lastname: Sun
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Chenjun
authors.initials: C
authors.lastname: Bai
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Shen
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Fang
authors.affiliation: Deparment of Orthopedics, Wuhan City Hospital No,3&Tongren Hospital of Wuhan University, Wuhan, 430060, China.
authors.forename: Zhanghua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Institute of Laboratory of Blood-borne Virus, Beijing, 100850, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Beijing Institute of Laboratory of Blood-borne Virus, Beijing, 100850, China.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China. Electronic address: shhli55@163.com.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory for Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, No. 27 Taiping Road, Haidian District, 100850, Beijing, China. Electronic address: xiejw@bmi.ac.cn.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Xie
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, 100850, China. Electronic address: shaonsh@hotmail.com.
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016503:Drug Delivery Systems; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000378:metabolism*; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D058726:Nanoconjugates / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051381:Rats
chemical_list: D052157:Aptamers, Nucleotide; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D058726:Nanoconjugates
keywords: Aptamer; Heterogeneous nuclear ribonucleoprotein A2/B1; Nanoparticles; SELEX; Tumor cells