title: NMR based biomarkers to study age-related changes in the human quadriceps.
abstract: Age-related sarcopenia is a major health issue. To improve elderly person quality of life, it is important to characterize age-associated structural changes within the skeletal muscle. NMR imaging offers quantitative tools to monitor these changes. We scanned 93 subjects: 33 young adults aged between 19 and 27 years old and 60 older adults between 69 and 80 years old. Their physical activity was assessed using a tri-axial accelerometer and they were classified either as active or sedentary. A standard multi-slice multi-echo (MSME) sequence was run and water T2 maps were extracted using a tri-exponential fit. Fat fraction was quantified using three-point Dixon technique. Each quadriceps muscle was characterized by: water T2 mean value, water T2 heterogeneity and the mean fat fraction. Statistical analysis (ANOVA) showed that water T2 mean values and its heterogeneity indices as well as fat fraction were significantly higher in the elderly group (p<0.05). Only fat fraction was significantly lower in the active group compared to the sedentary one (p<0.05). Linear regression confirmed the significant impact of age on these NMR parameters whereas physical activity impact was not systematic. NMR imaging provided a comprehensive assessment of the aging process impact on skeletal muscle composition. Water T2 increase might be related to changes in fiber typology while increased T2 heterogeneities might correlate with some degree of tissue disorganization, like the development of interstitial fibrosis. Fat fraction and water T2 heterogeneity increase was partly slowed down by physical activity. These changes were not gender dependent.
authors.affiliation: Institute of Myology, NMR Laboratory, Paris, France; CEA, I(2)BM, MIRCen, NMR Laboratory, Paris, France. Electronic address: n.azzabou@institut-myologie.org.
authors.forename: Noura
authors.initials: N
authors.lastname: Azzabou
authors.affiliation: Institute of Myology, Neuromuscular Physiology and Evaluation Laboratory, Paris, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Hogrel
authors.affiliation: Institute of Myology, NMR Laboratory, Paris, France; CEA, I(2)BM, MIRCen, NMR Laboratory, Paris, France.
authors.forename: Pierre G
authors.initials: PG
authors.lastname: Carlier
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D009043:Motor Activity / Q000502:physiology; D052097:Quadriceps Muscle / Q000378:metabolism / Q000473:pathology*; D055948:Sarcopenia / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D012727:Sex Characteristics; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Aging; Biomarkers; Fatty tissue; NMR imaging; Sarcopenia; Skeletal muscle