title: Curcumin Triggers DNA Damage and Inhibits Expression of DNA Repair Proteins in Human Lung Cancer Cells.
abstract: The study goal was to evaluate the effects of curcumin on DNA damage and expression of DNA-repair proteins in human lung cancer. Thus, NCI-H460 cells were used to study the effects of curcumin on DNA damage and repair in vitro. We investigated curcumin induces DNA damage by comet the assay and 4',6-diamidino-2-phenylindole (DAPI) staining. The DNA damage/repair-related protein levels were examined and monitored by western blotting and confocal microscopy. Curcumin significantly increased the length of comet tails and DNA condensation in NCI-H460 cells. Curcumin reduced expression of DNA-repair proteins such as 14-3-3 protein sigma (14-3-3σ), O6-methylguanine-DNA methyltransferase (MGMT), breast cancer susceptibility gene 1 (BRCA1), and mediator of DNA damage checkpoint 1 (MDC1). Curcumin also increased phosphorylation of p53 and Histone H2A.X (S140) in the nuclei of NCI-H460 cells. Taken together, our findings indicated that curcumin triggered DNA damage and inhibited expression of DNA-repair-associated proteins in NCI-H460 cells.
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan, R.O.C. Department of Medical Imaging and Radiology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Ting
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan, R.O.C.
authors.forename: Hsin-Ell
authors.initials: HE
authors.lastname: Wang
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Yu
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Hsin-Chung
authors.initials: HC
authors.lastname: Liu
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan, R.O.C. Department of Radiation Oncology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C. Department of Radiological Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. john740604@yahoo.com.tw.
authors.forename: I-Tsang
authors.initials: IT
authors.lastname: Chiang
mesh_terms: D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D010766:Phosphorylation / Q000187:drug effects / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; D003474:Curcumin
keywords: Curcumin; DNA damage; DNA repair; human lung cancer