title: [Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer].
abstract: OBJECTIVE
  The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers.
  
  
  METHODS
  The material included endometrial adenocarcinoma tissue samples of histopathological grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation, what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal endometrium.
  
  
  RESULTS
  The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial cancer samples as compared to the control at p-value<0.05 and FC(log2)>1.5. These genes were identified as differentially expressed in grade G2-ASMTL, GNA 11, PER2, PTGDS and in grade G3-GNA12, GNA 11. Silencing of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups, independently of the histopathological grade.
  
  
  CONCLUSIONS
  The profile expression of genes associated with regulation of melatonin receptors activity was different and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and prognostic marker Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and melatonin signal transmitters (GNA 11, GNA 12, RTGS).
authors.forename: Andrzej
authors.initials: A
authors.lastname: Witek
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Jęda
authors.forename: Michał
authors.initials: M
authors.lastname: Baliś
authors.forename: Joanna
authors.initials: J
authors.lastname: Orchel
authors.forename: Bartosz
authors.initials: B
authors.lastname: Sikora
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Skubis
authors.forename: Justyna
authors.initials: J
authors.lastname: Szota-Czyż
authors.forename: Tomasz
authors.initials: T
authors.lastname: Janikowski
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
mesh_terms: D018269:Carcinoma, Endometrioid / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D044094:Receptors, Melatonin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D044094:Receptors, Melatonin