title: Synthesis, crystal structures, photoluminescence properties and DNA binding of triazine-nickel(II) complexes for DNA detection.
abstract: We report here the synthesis of three new nickel(II) complexes: [Ni(PzTA)2CO3]·5H2O (PzTA=2,4-diamino-6-(2'-pyrazin)-1,3,5-triazine) in 1, [NiQ(PyTA)(H2O)2]Cl·H2O (HQ=8-hydroxyquinoline, PyTA=2,4-diamino-6-(2'-pyridyl)-1,3,5-triazine) in 2, [NiQ(PzTA)(H2O)2]Cl·H2O in 3, and they were characterized by UV spectroscopy, elemental analysis, molar conductivity and X-ray single crystal diffraction. Binding of the complexes to ct-DNA was investigated with electronic spectroscopy, ethidium bromide displacement from DNA, viscometry and cyclic voltammetry. The results depicted the DNA binding mode of the three complexes was intercalation, and complex 1 together with external static-electricity. Moreover, the three complexes also presented potential anti-oxidant activity. Interestingly, we found 1 was sensitive to oxygen and to the polarity of nonaqueous solvents in fluorescence spectroscopy. Fluorescence of 2 and 3 is weak in neutral aqueous solvents, but is greatly enhanced by addition of ct-DNA. Thus, 2 and 3 can be used to DNA detection as DNA fluorescence probes with a LOD of 1.61 ng mL(-1), 4.90 ng mL(-1) for the relative wide linear range of 0.01-20 μg mL(-1), 0.02-30 μg mL(-1), respectively. These findings indicate that 1 may be a potential optical probe for oxygen-free environments in nonaqueous form, while 2 and 3 were DNA-targeted probes.
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Ran-Ran
authors.initials: RR
authors.lastname: Duan
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Ou
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: xhzhou@scau.edu.cn.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhou
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry; D018360:Crystallography, X-Ray; D004247:DNA / Q000032:analysis* / Q000378:metabolism*; D007364:Intercalating Agents / Q000737:chemistry*; D008027:Light; D008163:Luminescent Measurements / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D014227:Triazines; D009532:Nickel; D004247:DNA
keywords: 1,3,5-Triazine; 8-Hydroxyquinoline; DNA interaction; Fluorescent probe; Nickel(II) complex