title: [Effect of Bear Bile Powder and Its Succedaneum on Peripheral Blood and Bone Marrow Megakaryocytes in Mouse Model with Thrombocytopenia Induced by Cytosine Arabinoside].
abstract: OBJECTIVE
  To investigate the effect of bear bile powder and ursodesxy cholic acid (UDCA) on peripheral blood, bone marrow megakaryocyte and immune organs in mouse model with thrombocytopenia, so as to provide a reference for studying the curative effects of bear bile powder and its succedaneum on thrombocytopenic purpura (TP).
  
  
  METHODS
  The mouse model with thrombocytopenia indued by cytosine arabinoside (Ara-C) was established, a total of 70 mice were randomly divided into normal group, model group, prednisone group, bear bile (middle and high dose) powder group and UDCA (middle and high dose) group. From the first day of making model mice in the each group, 0.4 ml/(20 g·d) corresponding drug was administered by infusion. At day 10 after treatment the peripheral blood, spleen and thymus organ index, the number of bone marrow megakaryocyte in each group were compared.
  
  
  RESULTS
  compared with the normal group, the Plt, WBC and megakaryocyte counts in model group decreased, the spleen index increased obviously (P<0.05), but the WBC count returned to normal by 10 days; after treatment, compared with model group, the Plt, WBC and megakaryocyte counts of treated groups increased, spleen index decreased significantly (P<0.05), but the WBC count in prednisone group decreased, which in bear bile powder (high) group and UDCA (high) group were particularly significant.
  
  
  CONCLUSIONS
  The bear bile powder and UDCA have been confirmed to have therapeutical effect on thrombocytopenia models induced by Ara-C, UDCA can substitute bear bile powder as a treatment drug for thrombocytopenic purpura.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Qing-Chun
authors.initials: QC
authors.lastname: Wang
authors.affiliation: Acadmy of Mongolian Medicine, Inner Mongolia Medical University, Hohhot 010020, Inner Mongolia, China.
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: Zhu
authors.affiliation: Department of Ultrasound, International Mongolian Medicine Hospital of Inner Mongolia, Hohhot 010056, Inner Mongolia, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Acadmy of Mongolian Medicine, Inner Mongolia Medical University, Hohhot 010020, Inner Mongolia, China. E-mail: changbai2010@aliyun.com.
authors.forename: Chang-Xi
authors.initials: CX
authors.lastname: Bai
mesh_terms: D000818:Animals; D001646:Bile; D001853:Bone Marrow; D001854:Bone Marrow Cells; D003561:Cytarabine; D004195:Disease Models, Animal; D008533:Megakaryocytes; D051379:Mice; D013154:Spleen; D013921:Thrombocytopenia
chemical_list: D003561:Cytarabine