title: Sevoflurane Induces DNA Damage Whereas Isoflurane Leads to Higher Antioxidative Status in Anesthetized Rats.
abstract: Taking into account that there are controversial antioxidative effects of inhalational anesthetics isoflurane and sevoflurane and absence of comparison of genotoxicity of both anesthetics in animal model, the aim of this study was to compare DNA damage and antioxidant status in Wistar rats exposed to a single time to isoflurane or sevoflurane. The alkaline single-cell gel electrophoresis assay (comet assay) was performed in order to evaluate DNA damage in whole blood cells of control animals (unexposed; n = 6) and those exposed to 2% isoflurane (n = 6) or 4% sevoflurane (n = 6) for 120 min. Plasma antioxidant status was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was no statistically significant difference between isoflurane and sevoflurane groups regarding hemodynamic and temperature variables (P > 0.05). Sevoflurane significantly increased DNA damage compared to unexposed animals (P = 0.02). In addition, Wistar rats anesthetized with isoflurane showed higher antioxidative status (MTT) than control group (P = 0.019). There were no significant differences in DNA damage or antioxidant status between isoflurane and sevoflurane groups (P > 0.05). In conclusion, our findings suggest that, in contrast to sevoflurane exposure, isoflurane increases systemic antioxidative status, protecting cells from DNA damage in rats.
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-000 Botucatu, SP, Brazil ; Department of Anesthesiology, Botucatu Medical School, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil.
authors.forename: Thalita L A
authors.initials: TL
authors.lastname: Rocha
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-000 Botucatu, SP, Brazil ; Department of Anesthesiology, Botucatu Medical School, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Dias-Junior
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-000 Botucatu, SP, Brazil.
authors.forename: Jose S
authors.initials: JS
authors.lastname: Possomato-Vieira
authors.affiliation: Department of Pharmacology, Biosciences Institute of Botucatu, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-000 Botucatu, SP, Brazil.
authors.forename: Victor H
authors.initials: VH
authors.lastname: Gonçalves-Rizzi
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil.
authors.forename: Flávia R
authors.initials: FR
authors.lastname: Nogueira
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil.
authors.forename: Kátina M
authors.initials: KM
authors.lastname: de Souza
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil.
authors.forename: Leandro G
authors.initials: LG
authors.lastname: Braz
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, Sao Paulo State University (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Braz
mesh_terms: D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000009:adverse effects / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D004195:Disease Models, Animal; D007530:Isoflurane / Q000008:administration & dosage* / Q000009:adverse effects; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage* / Q000009:adverse effects; D051381:Rats; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D000975:Antioxidants; D008738:Methyl Ethers; D000077149:Sevoflurane; D007530:Isoflurane