title: Antitumoral Effect of Mural Cells Assessed With High-Resolution MRI and Fluorescence Microscopy.
abstract: OBJECTIVE
  The purpose of this study was to detect labeled mural cells in vivo and study their therapeutic effect on tumor growth and on functional changes in the vascular network by use of MRI and fibered confocal fluorescence microscopy (FCFM).
  
  
  METHODS
  Twenty-eight mice were allocated to the following three groups 7 days after injection of TC1 tumor cells (C157 black 6): control, no injection (n = 7); sham, injection of phosphate-buffered saline solution (n = 10); and treated, injection of human mural cells (n = 11). Tumor growth was measured with calipers. Labeled mural cells were tracked with high-resolution MRI and FCFM. Microvessel density was assessed with MRI and FCFM, and the findings were compared with the histologic results.
  
  
  RESULTS
  Tumor growth was significantly slowed in the treated group starting on day 10 (p = 0.001). Round signal-intensity voids were observed in the center of six of seven tumors treated with magnetically labeled mural cells. Positive staining for iron was observed in histologic sections of two of five of these tumors. Microvessel density measured with FCFM was greater in the treated mice (p = 0.03). Flow cytometry revealed viable human mural cells only in treated tumors.
  
  
  CONCLUSIONS
  In this study, imaging techniques such as high-resolution MRI and FCFM showed the therapeutic effect of mural cell injection on tumor growth and microvessel function.
authors.affiliation: 1 Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970, 56 rue Leblanc, 75015 Paris, France.|2 Women's Medical Imaging, CHRU Lille, Hôpital Jeanne de Flandre, Lille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Faye
authors.affiliation: 1 Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970, 56 rue Leblanc, 75015 Paris, France.|3 Radiology Department, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Fournier
authors.affiliation: 4 Plateforme d'Imagerie du Petit Animal, Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Balvay
authors.affiliation: 5 Laboratoire Matière et Systèmes Complexes, Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Wilhelm
authors.affiliation: 6 Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France.
authors.forename: Dong
authors.initials: D
authors.lastname: Broqueres-You
authors.affiliation: 7 Pathology Laboratory, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Bruneval
authors.affiliation: 1 Cardiovascular Research Center-PARCC, Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970, 56 rue Leblanc, 75015 Paris, France.|3 Radiology Department, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Clément
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003287:Contrast Media / Q000008:administration & dosage; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008833:Microcirculation; D018613:Microscopy, Confocal; D009389:Neovascularization, Pathologic / Q000473:pathology*; D020286:Pericytes / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D003287:Contrast Media
keywords: MRI; cellular imaging; fluorescence microscopy; mural cells; tumor angiogenesis