title: Highly Active Biocatalytic Coatings from Protein-Polymer Diblock Copolymers.
abstract: A method for fabricating nanostructured biocatalysts using bioconjugate block copolymer self-assembly is demonstrated, yielding very high protein loadings and activity per unit area, compared to more-established enzyme encapsulation methods. Self-assembled heterogeneous biocatalysts are fabricated by flow coating myoglobin-b-poly(N-isopropylacrylamide) (myoglobin-PNIPAM) block copolymers onto solid supports, and films are stabilized by lightly cross-linking with glutaraldehyde. The conjugates form weakly ordered, nonbirefringent micellar and lamellar assemblies in concentrated solution and disordered but micro-phase-separated structures in thin solid films. The low diffusion resistance in the bioconjugate film imparted by the water-swollen PNIPAM nanostructures, the high enzyme density within the film, and high retention of protein activity results in extremely high catalytic activity: 5-10 times greater than catalysts fabricated using other well-established methods.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Aaron
authors.initials: A
authors.lastname: Huang
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Guokui
authors.initials: G
authors.lastname: Qin
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Bradley D
authors.initials: BD
authors.lastname: Olsen
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D002384:Catalysis; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D008422:Materials Testing; D008567:Membranes, Artificial; D009211:Myoglobin / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D009211:Myoglobin; C052970:poly-N-isopropylacrylamide
keywords: biocatalyst; bioconjugation; block copolymer; myoglobin