title: A novel variant of the β-lactamase ADC-61 gene in multi-drug resistant Acinetobacter baumannii.
abstract: The aim of this study was to investigate the existence of a β-lactamase gene in a group of multi-drug resistant Acinetobacter baumannii. Twenty strains of multi-drug resistant A. baumannii were isolated. Thirty-four β-lactamase genes and the ISaba1-OXA-23 linkage were analyzed in these strains by polymerase chain reaction (PCR) and verified by DNA sequencing. Three kinds of β-lactamase genes (TEM, ADC, and OXA-23) were identified, among which the sequence of strain No. 20, ADC, was different from ADC subtypes recorded by GenBank, and was identified as a new variant of β-lactamase genes (named ADC-61 and registered in GenBank: accession No. JQ753702); all the other 19 strains were ADC-30. Eighteen strains of the OXA-23 group were all positive as indicated by detection of ISaba1-OXA-23 linkage. Gene sequencing indicated that the TEM gene was TEM-1. These results suggest that the three kinds of β-lactamase genes identified in this study, TEM, ADC, and OXA-23, play a key role in drug resistance in this group of A. baumannii. To our knowledge, this is the first report of an emergent new mutation of the β-lactamase gene, ADC-61, in China or abroad.
authors.affiliation: Clinical Laboratory Department, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Clinical Laboratory Department, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu, China.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Teng
authors.affiliation: Clinical Laboratory Department, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Clinical Laboratory Department, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu, China.
authors.forename: S-B
authors.initials: SB
authors.lastname: Li
authors.affiliation: Clinical Laboratory Department, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001618:beta-Lactamases