title: Preserved Cerebral Microcirculation After Cardiac Arrest in a Rat Model.
abstract: OBJECTIVE
  Recent studies show that sublingual microcirculation is altered in patients resuscitated from CA. The objective of this study was to investigate whether the cerebral microcirculation is disturbed in the early post-resuscitation period.
  
  
  METHODS
  Male Sprague-Dawley rats were randomized to either 10 minutes of CA or uninterrupted circulation, and observed to 120 or 360 minutes after ROSC. At 120 and 360 minutes, cerebral microcirculation was evaluated by SDF microscopy through a craniectomy. Plasma samples were analyzed for endothelial adhesion molecules and inflammatory markers, and brains were fixated for histological analysis.
  
  
  RESULTS
  Cerebral microcirculation, evaluated by TVD, PVD, PPV, and MFI did not differ between groups (p > 0.16). Plasma samples drawn 360 minutes after ROSC displayed a significant increase in sE-selectin, sL-selectin, sI-CAM1, IL-1β, IL-6, IL-10, and elastase compared to controls. In the CA animals, sE-selectin and elastase increased between 120 and 360 minutes after resuscitation (p < 0.007). Histological analysis revealed neuronal death in hippocampus layer CA1 360 min after resuscitation.
  
  
  CONCLUSIONS
  When evaluated by SDF, the cerebral microcirculation appears unaffected in the early post-CA period despite hypotension, systemic inflammation, endothelial activation, and neuronal injury.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Aarhus University Hospital, Aarhus C, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Secher
authors.affiliation: Center of Functionally Integrative Neuroscience, Aarhus University, Aarhus C, Denmark.
authors.forename: Leif
authors.initials: L
authors.lastname: Østergaard
authors.affiliation: Center of Functionally Integrative Neuroscience, Aarhus University, Aarhus C, Denmark.
authors.forename: Nina K
authors.initials: NK
authors.lastname: Iversen
authors.affiliation: Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense C, Denmark.
authors.forename: Kate L
authors.initials: KL
authors.lastname: Lambertsen
authors.affiliation: Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense C, Denmark.
authors.forename: Bettina H
authors.initials: BH
authors.lastname: Clausen
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Aarhus University Hospital, Aarhus C, Denmark.
authors.forename: Else
authors.initials: E
authors.lastname: Tønnesen
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Aarhus University Hospital, Aarhus C, Denmark.
authors.forename: Asger
authors.initials: A
authors.lastname: Granfeldt
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D006323:Heart Arrest / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D008833:Microcirculation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators
keywords: cardiac arrest; endothelial activation; inflammation; microcirculation; resuscitation