title: Novel Molecular Assay for Simultaneous Identification of Neglected Lungworms and Heartworms Affecting Cats.
abstract: Feline lungworms and heartworms are stimulating the interest of the scientific community due to their clinical impact and apparent geographical expansion. Diagnosis of the infections caused by these nematodes is indeed challenging. This report describes a novel multiplex PCR able to identify simultaneously three species of lungworms (Aelurostrongylus abstrusus and Troglostrongylus brevior) and heartworms (Angiostrongylus chabaudi) affecting felids. Epidemiological and clinical perspectives are discussed.
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy angdicesare@gmail.com.
authors.forename: Angela
authors.initials: A
authors.lastname: Di Cesare
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Veronesi
authors.affiliation: Department of Animal Medicine, Production and Health, University of Padua, Padua, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Frangipane di Regalbono
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Iorio
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors.forename: Donato
authors.initials: D
authors.lastname: Traversa
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000469:parasitology; D002415:Cats; D006374:Helminthiasis, Animal / Q000175:diagnosis* / Q000469:parasitology; D008681:Metastrongyloidea / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D014730:Veterinary Medicine / Q000379:methods*