title: Serum Albumin-Alginate Microparticles Prepared by Transacylation: Relationship between Physicochemical, Structural and Functional Properties.
abstract: Our laboratory develops a method of microencapsulation using a transacylation reaction in a water-in-oil (W/O) emulsion. The method is based on the creation of amide bonds between free amine functions of a protein (human serum albumin (HSA)) and ester groups of propylene glycol alginate (PGA) in the inner aqueous phase after alkalization. The aim of this work is to study the influence of physicochemical properties of HSA-PGA mixtures on microparticle characteristics. Microparticles were prepared varying the concentrations of PGA and HSA, then characterized (inner structure, size, swelling rate, release kinetics). PGA and each polymer mixture used in the microencapsulation procedure were examined in order to elucidate the mechanism of microstructure formation. It was found that the morphology and functional properties of HSA-alginate microparticles were related to the two polymer concentrations in the aqueous solution. Actually, the polymer concentration variations led to physicochemical changes, which affected the microparticle structure and functional properties.
authors.affiliation: †Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 7312, U.F.R. Pharmacie, 51, rue Cognacq-Jay, 51096 Reims, France.|‡Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 7312, U.F.R. Sciences Exactes et Naturelles, Campus du Moulin de la Housse 51687 Reims, France.
authors.forename: Imane
authors.initials: I
authors.lastname: Hadef
authors.affiliation: ‡Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 7312, U.F.R. Sciences Exactes et Naturelles, Campus du Moulin de la Housse 51687 Reims, France.
authors.forename: Barbara
authors.initials: B
authors.lastname: Rogé
authors.affiliation: †Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 7312, U.F.R. Pharmacie, 51, rue Cognacq-Jay, 51096 Reims, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Edwards-Lévy
mesh_terms: D000215:Acylation; D000464:Alginates / Q000737:chemistry* / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry* / Q000627:therapeutic use; D004339:Drug Compounding; D006801:Humans; D011108:Polymers / Q000737:chemistry* / Q000627:therapeutic use; D012709:Serum Albumin / Q000737:chemistry* / Q000627:therapeutic use; D014867:Water / Q000737:chemistry
chemical_list: D000464:Alginates; D004337:Drug Carriers; D011108:Polymers; D012709:Serum Albumin; D014867:Water; C038550:propylene glycol alginate ester