title: Preferential recruitment of the maternal centromere-specific histone H3 (CENH3) in oat (Avena sativa L.) × pearl millet (Pennisetum glaucum L.) hybrid embryos.
abstract: Chromosome elimination occurs frequently in interspecific hybrids between distantly related species in Poaceae. However, chromosomes from both parents behave stably in a hybrid of female oat (Avena sativa L.) pollinated by pearl millet (Pennisetum glaucum L.). To analyze the chromosome behavior in this hybrid, we cloned the centromere-specific histone H3 (CENH3) genes of oat and pearl millet and produced a pearl millet-specific anti-CENH3 antibody. Application of this antibody together with a grass species common anti-CENH3 antibody revealed the dynamic CENH3 composition of the hybrid cells before and after fertilization. Despite co-expression of CENH3 genes encoded by oat and pearl millet, only an oat-type CENH3 was incorporated into the centromeres of both species in the hybrid embryo. Oat CENH3 enables a functional centromere in pearl millet chromosomes in an oat genetic background. Comparison of CENH3 genes among Poaceae species that show chromosome elimination in interspecific hybrids revealed that the loop 1 regions of oat and pearl millet CENH3 exhibit exceptionally high similarity.
authors.affiliation: Arid Land Research Center, Tottori University, Hamasaka, Tottori, 680-0001, Japan.|Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, 06466, Gatersleben, Germany.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Ishii
authors.affiliation: Faculty of Medicine, Tottori University, Yonago, Tottori, 683-8503, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sunamura
authors.affiliation: Arid Land Research Center, Tottori University, Hamasaka, Tottori, 680-0001, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Arid Land Research Center, Tottori University, Hamasaka, Tottori, 680-0001, Japan.
authors.forename: Amin Elsadig
authors.initials: AE
authors.lastname: Eltayeb
authors.affiliation: Arid Land Research Center, Tottori University, Hamasaka, Tottori, 680-0001, Japan. tsujim@alrc.tottori-u.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D018554:Avena / Q000235:genetics* / Q000378:metabolism; D002503:Centromere / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D015870:Gene Expression; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D031782:Pennisetum / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D012639:Seeds; D016415:Sequence Alignment
chemical_list: D006657:Histones; D010940:Plant Proteins
keywords: centromere-specific histone H3 (CENH3); chromosome elimination; embryogenesis; interspecific cross; oat; pearl millet