title: Regulating effects of insertion direction of matrix attachment regions on transgenic expression in stably transformed Chinese hamster ovary cells.
abstract: We investigated the effects of different directions of insertion of matrix attachment region (MAR) sequences on transgenic expression in stably transformed Chinese hamster ovary (CHO) cells. The MAR sequences were inserted in forward or reverse directions into the expression vectors, and transfected into CHO cells. The expression of the chloramphenicol acetyltransferase (CAT) reporter gene and the relative copy numbers of the CAT gene were analyzed. The CAT gene expression levels in the vector with the MAR sequence inserted in the forward or reverse directions increased compared with expression without the MAR sequence. The relative copy numbers of the CAT gene with MAR sequenced vectors inserted in the reverse and forward directions were lower, than in the control group. The direction of insertion of MAR sequences had no significant effect on expression levels. The expression levels were not proportional to the copy numbers of the gene.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China wtianyuncn@126.com.
authors.forename: T Y
authors.initials: TY
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Dong
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: R
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: C P
authors.initials: CP
authors.lastname: Zhao
mesh_terms: D000818:Animals; D016466:CHO Cells; D002461:Cell Line, Transformed; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D003412:Cricetulus; D021901:DNA, Intergenic / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism; D045170:Matrix Attachment Regions; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D019076:Transgenes
chemical_list: D021901:DNA, Intergenic; D015500:Chloramphenicol O-Acetyltransferase