title: PHOTOSYNTHESIS. A 12 Å carotenoid translocation in a photoswitch associated with cyanobacterial photoprotection.
abstract: Pigment-protein and pigment-pigment interactions are of fundamental importance to the light-harvesting and photoprotective functions essential to oxygenic photosynthesis. The orange carotenoid protein (OCP) functions as both a sensor of light and effector of photoprotective energy dissipation in cyanobacteria. We report the atomic-resolution structure of an active form of the OCP consisting of the N-terminal domain and a single noncovalently bound carotenoid pigment. The crystal structure, combined with additional solution-state structural data, reveals that OCP photoactivation is accompanied by a 12 angstrom translocation of the pigment within the protein and a reconfiguration of carotenoid-protein interactions. Our results identify the origin of the photochromic changes in the OCP triggered by light and reveal the structural determinants required for interaction with the light-harvesting antenna during photoprotection.
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Ryan L
authors.initials: RL
authors.lastname: Leverenz
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Sutter
authors.affiliation: Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay (iBiTec-S), 91191 Gif-sur-Yvette, France. Centre National de la Recherche Scientifique (CNRS), I2BC, UMR 9198, 91191 Gif-sur-Yvette, France.
authors.forename: Adjélé
authors.initials: A
authors.lastname: Wilson
authors.affiliation: Berkeley Center for Structural Biology, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Sayan
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay (iBiTec-S), 91191 Gif-sur-Yvette, France. Centre National de la Recherche Scientifique (CNRS), I2BC, UMR 9198, 91191 Gif-sur-Yvette, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Thurotte
authors.affiliation: Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay (iBiTec-S), 91191 Gif-sur-Yvette, France. Centre National de la Recherche Scientifique (CNRS), I2BC, UMR 9198, 91191 Gif-sur-Yvette, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Bourcier de Carbon
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Petzold
authors.affiliation: Berkeley Center for Structural Biology, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Corie
authors.initials: C
authors.lastname: Ralston
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: François
authors.initials: F
authors.lastname: Perreau
authors.affiliation: Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay (iBiTec-S), 91191 Gif-sur-Yvette, France. Centre National de la Recherche Scientifique (CNRS), I2BC, UMR 9198, 91191 Gif-sur-Yvette, France.
authors.forename: Diana
authors.initials: D
authors.lastname: Kirilovsky
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA. ckerfeld@lbl.gov.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Kerfeld
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D016644:Canthaxanthin / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D008956:Models, Chemical; D010788:Photosynthesis; D045524:Phycobilisomes / Q000737:chemistry*; D017433:Protein Structure, Secondary; D021381:Protein Transport; D046939:Synechocystis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D045524:Phycobilisomes; C109473:orange carotenoid protein, Synechocystis; D016644:Canthaxanthin