title: A new curvularin glycoside and its cytotoxic and antibacterial analogues from marine actinomycete Pseudonocardia sp. HS7.
abstract: Five curvularin macrolides (1-5) were isolated from the cultured broth of marine actinomycete Pseudonocardia sp. HS7 that was obtained from the cloacal aperture of sea cucumber Holothuria moebii. The structures of these isolates were characterized as (11S,15R)-11-hydroxycurvularin (1), (11R,15R)-11-hydroxycurvularin (2), curvularin-7-O-α-D-glucopyranoside (3), trans-dehydrocurvularin (4) and curvularin (5) based on their NMR and HRESIMS data as well as chemical degradation. Compound 3 is a new macrolide with a rare α-D-glucopyranose substituent. Compounds 1-4, 5a and 5c (the acyl products of 5), suppressed the proliferation of all six tested cancer cell lines and 4 is the most active compound with IC50 values ranging from 0.59 to 3.39 μM. The 11-hydroxycurvularins 1 and 2 also showed antibacterial activity inhibiting the growth of Escherichia coli.
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Ye
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Komal
authors.initials: K
authors.lastname: Anjum
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Song
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Wang
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Siran
authors.initials: S
authors.lastname: Yu
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Haocai
authors.initials: H
authors.lastname: Huang
authors.affiliation: b College of Pharmaceutical Sciences, Zhejiang University, , Hangzhou 310058 , P.R. China.
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Lian
authors.affiliation: a Ocean College, Zhejiang University , Hangzhou 310058 , P.R. China.
authors.forename: Zhizhen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D047332:Holothuria / Q000382:microbiology*; D006801:Humans; D020128:Inhibitory Concentration 50; D018942:Macrolides / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D013237:Stereoisomerism; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D006027:Glycosides; D018942:Macrolides; C013914:dehydrocurvularin; D015025:Zearalenone; C013455:curvularin
keywords: Pseudonocardia sp. HS7; antibacterial activity; curvularin glycoside; cytotoxic