title: The Variability of the Order Burkholderiales Representatives in the Healthcare Units.
abstract: OBJECTIVE
  The order Burkholderiales became more abundant in the healthcare units since the late 1970s; it is especially dangerous for intensive care unit patients and patients with chronic lung diseases. The goal of this investigation was to reveal the real variability of the order Burkholderiales representatives and to estimate their phylogenetic relationships.
  
  
  METHODS
  16S rDNA and genes of the Burkholderia cenocepacia complex (Bcc) Multi Locus Sequence Typing (MLST) scheme were used for the bacteria detection.
  
  
  RESULTS
  . A huge diversity of genome size and organization was revealed in the order Burkholderiales that may prove the adaptability of this taxon's representatives. The following variability of the Burkholderiales in Russian healthcare units has been revealed: Burkholderiaceae (Burkholderia, Pandoraea, and Lautropia), Alcaligenaceae (Achromobacter), and Comamonadaceae (Variovorax). The Burkholderia genus was the most diverse and was represented by 5 species and 16 sequence types (ST). ST709 and 728 were transmissible and often encountered in cystic fibrosis patients and in hospitals. A. xylosoxidans was estimated by 15 genotypes. The strains of first and second ones were the most numerous.
  
  
  CONCLUSIONS
  Phylogenetic position of the genus Lautropia with smaller genome is ambiguous. The Bcc MLST scheme is applicable for all Burkholderiales representatives for resolving the epidemiological problems.
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Olga L
authors.initials: OL
authors.lastname: Voronina
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Marina S
authors.initials: MS
authors.lastname: Kunda
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Ryzhova
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Ekaterina I
authors.initials: EI
authors.lastname: Aksenova
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Semenov
authors.affiliation: Federal State Budgetary Institution "Scientific Centre of Children Health" RAMS, 119991 Moscow, Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Lasareva
authors.affiliation: Research Institute of Pulmonology FMBA of Russia, 105077 Moscow, Russia.
authors.forename: Elena L
authors.initials: EL
authors.lastname: Amelina
authors.affiliation: Research Institute of Pulmonology FMBA of Russia, 105077 Moscow, Russia.
authors.forename: Alexandr G
authors.initials: AG
authors.lastname: Chuchalin
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Vladimir G
authors.initials: VG
authors.lastname: Lunin
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, 123098 Moscow, Russia.
authors.forename: Alexandr L
authors.initials: AL
authors.lastname: Gintsburg
mesh_terms: D019121:Burkholderia Infections / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D042521:Burkholderiaceae / Q000235:genetics* / Q000472:pathogenicity; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013045:Species Specificity
chemical_list: D012336:RNA, Ribosomal, 16S