title: Dissemination of the multidrug-resistant extended-spectrum β-lactamase-producing Escherichia coli O25b-ST131 clone and the role of house crow (Corvus splendens) foraging on hospital waste in Bangladesh.
abstract: Two hundred and thirty-eight faecal samples from crows foraging on hospital wastes were analysed for extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. ESBL-producing crow isolates were characterized and compared with 31 patient isolates. Among the crows, 59% carried ESBL producers. These included Escherichia coli, Klebsiella pneumoniae, Raoultella terrigena and Enterobacter cloacae harbouring the genes for CTX-M-1, CTX-M-15, CTX-M-55, CTX-M-79, and CTX-M-14. Human isolates carried only the CTX-M-15 gene. Two-thirds of crow E. coli isolates and all human E. coli isolates were multidrug resistant. Crows and patients shared E. coli sequence types, including the epidemic E. coli O25b-ST131 clone. The scavenging behaviour of crows at poorly managed hospital waste dumps made them potential reservoirs of antibiotic resistance, including ESBLs.
authors.affiliation: Clinical Microbiology and Infectious Medicine, Department of Medical Sciences, Uppsala University, Uppsala, Sweden; Zoonosis Science Centre, Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, Uppsala, Sweden. Electronic address: badrul.hasan@medsci.uu.se.
authors.forename: B
authors.initials: B
authors.lastname: Hasan
authors.affiliation: Clinical Microbiology and Infectious Medicine, Department of Medical Sciences, Uppsala University, Uppsala, Sweden; Zoonosis Science Centre, Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, Uppsala, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Olsen
authors.affiliation: Rajshahi Medical College Hospital, Rajshahi, Bangladesh.
authors.forename: A
authors.initials: A
authors.lastname: Alam
authors.affiliation: Chittagong Medical College Hospital, Chittagong, Bangladesh.
authors.forename: L
authors.initials: L
authors.lastname: Akter
authors.affiliation: Clinical Microbiology and Infectious Medicine, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Å
authors.initials: Å
authors.lastname: Melhus
mesh_terms: D000818:Animals; D001459:Bangladesh; D046368:Crows / Q000502:physiology*; D004197:Disease Reservoirs; D024901:Drug Resistance, Multiple, Bacterial; D016972:Enterobacter cloacae / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005247:Feeding Behavior; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification; D017744:Medical Waste Disposal; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D017744:Medical Waste Disposal; D001618:beta-Lactamases
keywords: E. coli; O25b-ST131; bla(CTX-M-15); hospital waste; house crow