title: Hydroxysafflor yellow A inhibits lipopolysaccharide-induced proliferation and migration of vascular smooth muscle cells via Toll-like receptor-4 pathway.
abstract: Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) is closely associated with early vascular hyperplasic lesions. Toll-like receptor (TLR)-4 is a pathogen pattern recognition receptor expressed on VSMCs, and can be activated by lipopolysaccharide. Activated TLR-4 plays a promoting role in VSMCs proliferation and migration through the downstream signaling pathways including Rac1/Akt. Hydroxysafflor yellow A (HSYA) is the main component of the safflower yellow pigments, which has long been used for the treatment of cardiovascular diseases in traditional Chinese medicine. However, the effect of HSYA on VSMC proliferation and migration remains unknown. In the present study, we showed that HYSA could inhibit LPS-induced VSMCs proliferation and migration, accompanied by the downregulated levels of several key pro-inflammatory cytokines, including TNF-α, IL-6, and IL-8. We further showed that HYSA inhibited LPS-induced upregulation of TLR-4 expression as well as the activation of Rac1/Akt pathway, suggesting that HSYA inhibits LPS-induced VSMCs proliferation and migration, partly at least, via inhibition of TLR-4/Rac1/Akt pathway. Accordingly, HSYA may be used as a promising agent for prevention and treatment of vascular hyperplasic disorders.
authors.affiliation: Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University (Haikou Municipal People's Hospital) Haikou 570208, Hainan Province, China.
authors.forename: Guoshuai
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Hemodialysis, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University (Haikou Municipal People's Hospital) Haikou 570208, Hainan Province, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The People's Hospital of Hainan Province Haikou 570311, Hainan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Neurology, The People's Hospital of Hainan Province Haikou 570311, Hainan Province, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University (Haikou Municipal People's Hospital) Haikou 570208, Hainan Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong Province, China.
authors.forename: Suyue
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University Changsha 410008, Hunan Province, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Song
keywords: Hydroxysafflor yellow A; lipopolysaccharide; migration; proliferation; vascular smooth muscle cell