title: Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.
abstract: CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.|‡Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, United States.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Veldkamp
authors.affiliation: §IST Austria (Institute for Science and Technology Austria), 3400 Klosterneuburg, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Kiermaier
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Skylar J
authors.initials: SJ
authors.lastname: Gabel-Eissens
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Miranda L
authors.initials: ML
authors.lastname: Gillitzer
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: David R
authors.initials: DR
authors.lastname: Lippner
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Frank A
authors.initials: FA
authors.lastname: DiSilvio
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Casey J
authors.initials: CJ
authors.lastname: Mueller
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Paeton L
authors.initials: PL
authors.lastname: Wantuch
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Chaffee
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Famiglietti
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Zgoba
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Asha A
authors.initials: AA
authors.lastname: Bailey
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Yaya
authors.initials: Y
authors.lastname: Bah
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Engebretson
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: David R
authors.initials: DR
authors.lastname: Graupner
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Emily R
authors.initials: ER
authors.lastname: Lackner
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Vincent D
authors.initials: VD
authors.lastname: LaRosa
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Tysha
authors.initials: T
authors.lastname: Medeiros
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Olson
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Phillips
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Harley
authors.initials: H
authors.lastname: Pyles
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Richard
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Schoeller
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Boris
authors.initials: B
authors.lastname: Touzeau
authors.affiliation: †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.
authors.forename: Larry G
authors.initials: LG
authors.lastname: Williams
authors.affiliation: §IST Austria (Institute for Science and Technology Austria), 3400 Klosterneuburg, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Sixt
authors.affiliation: ‡Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, United States.
authors.forename: Francis C
authors.initials: FC
authors.lastname: Peterson
mesh_terms: D001665:Binding Sites; D054415:Chemokine CCL19 / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D054400:Receptors, CCR7 / Q000378:metabolism*
chemical_list: C515370:CCR7 protein, human; D054415:Chemokine CCL19; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D054400:Receptors, CCR7