title: Quantitative analysis of flavanones from citrus fruits by using mesoporous molecular sieve-based miniaturized solid phase extraction coupled to ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry.
abstract: An analytical procedure based on miniaturized solid phase extraction (SPE) and ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was developed and validated for determination of six flavanones in Citrus fruits. The mesoporous molecular sieve SBA-15 as a solid sorbent was characterised by Fourier transform-infrared spectroscopy and scanning electron microscopy. Additionally, compared with reported extraction techniques, the mesoporous SBA-15 based SPE method possessed the advantages of shorter analysis time and higher sensitivity. Furthermore, considering the different nature of the tested compounds, all of the parameters, including the SBA-15 amount, solution pH, elution solvent, and the sorbent type, were investigated in detail. Under the optimum condition, the instrumental detection and quantitation limits calculated were less than 4.26 and 14.29ngmL(-1), respectively. The recoveries obtained for all the analytes were ranging from 89.22% to 103.46%. The experimental results suggested that SBA-15 was a promising material for the purification and enrichment of target flavanones from complex citrus fruit samples.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cao
authors.affiliation: Hangzhou Red Cross Hospital, Department of Traditional Chinese Medicine, Hangzhou 310003, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Peng
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Qiong-Yao
authors.initials: QY
authors.lastname: Zhu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: Zhang
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D044950:Flavanones; C509969:SBA-15; D012997:Solvents; D012822:Silicon Dioxide
keywords: Citrus fruits; Flavanones; Mesoporous molecular sieve; Miniaturized solid phase extraction; UHPLC-Q-TOF/MS