title: Depletion of enteroantigen-activated CD4⁺ T cells: effects on proliferation and pathogenicity.
abstract: Naive CD4(+) T cells depleted of natural Treg (CD25(+)) cells proliferate extensively when exposed to a fecal extract [enteroantigen (eAg)] pulsed on antigen-presenting cells (APC). When transplanted into SCID recipient mice, the CD25-depleted T cells induce a chronic colitis with a lethal course. We observed here that if T cells, pre-activated for 48h by eAg from BALB/c or SCID mice, are removed and then reexposed to either of the two sources of antigen, these T cells have completely lost their anti-eAg proliferative capacity in vitro. This observation indicates that eAgs derived from Balb/c and SCID mice are recognized by similar subsets of T cells. However, when transferred into SCID mice, eAg-activation-depleted T cells are still capable of inducing a severe colitis fully comparable with the disease induced by naive CD4(+) T cells.
authors.affiliation: Department of Immunology and Microbiology, Faculty of Health Sciences, Panum Institute, University of Copenhagen, Blegdamsvej 3, Copenhagen 2200, Denmark.
authors.forename: Mohammed-Samir
authors.initials: MS
authors.lastname: Belmaáti
authors.affiliation: Department of Immunology and Microbiology, Faculty of Health Sciences, Panum Institute, University of Copenhagen, Blegdamsvej 3, Copenhagen 2200, Denmark claesson@sund.ku.dk.
authors.forename: Mogens H
authors.initials: MH
authors.lastname: Claesson
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation / Q000502:physiology*; D003092:Colitis / Q000276:immunology; D005260:Female; D008213:Lymphocyte Activation / Q000276:immunology*; D008212:Lymphocyte Depletion / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014774:Virulence / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial
keywords: SCID; T cells; colitis