title: [Affinity Detection and Quantification of Receptors of TRAIL Based on Their Thermostability].
abstract: OBJECTIVE
  To develop a way for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors quantification in cancer via its' thermostability.
  
  
  METHODS
  Endougenous alkaline phosphatase (AP) activity and denaturation temperature of pancreatic cancer cell lines AsPC-1 and Capan-2 were detected. Boiling treated recombinant protein death receptor 5 (DR5), named DR5 AP, as well as pancreatic cancer cells lines AsPC-1 and Capan-2 were incubated with AP-tagged TRAIL (AP-TRAIL), and then reacted with Reagent A and Reagent S, the substrate of AP, to quantitive and in site detection of the receptor.
  
  
  RESULTS
  The endougenous AP activity of pancreatic cancer cells lines AsPC-1 and Capan-2 could not be totally inactivated by incubated at 65 °C, thus inhibited the detection of TRAIL receptor, but the activity was dramatically decreased after treated with boiling water, whereas the DR5-AP was thermal stable. The surface receptor of AsPC-1 and Capan-2 could be recognized and bound by AP-TRAIL after treated at 100 °C, the readings were 2. 210±0. 393 and 2. 027±0. 019.
  
  
  CONCLUSIONS
  The TRAIL receptors are thermostable and this may provide a better diagnosis and prognosis of cancer as well as personalize cancer therapy.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Hai-peng
authors.initials: HP
authors.lastname: Liu
authors.forename: Tian-zhen
authors.initials: TZ
authors.lastname: Long
authors.forename: Guang-ping
authors.initials: GP
authors.lastname: Luo
authors.forename: Peng
authors.initials: P
authors.lastname: Liang
mesh_terms: D045744:Cell Line, Tumor; D006358:Hot Temperature; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D055550:Protein Stability; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D011994:Recombinant Proteins