title: Antidermatophytic Activity of Mikania micrantha Kunth: An Invasive Weed.
abstract: BACKGROUND
  The incidence of dermatophytosis has risen dramatically in recent years. Limited availability of side-effect free drugs has led to a search for new antidermatophytic agents.
  
  
  OBJECTIVE
  The objective was to investigate antidermatophytic activity and in vitro anti-inflammatory activity (protease inhibition assay) of whole plant (aerial parts only) of Mikania micrantha.
  
  
  METHODS
  The dried and powdered aerial parts of M. micrantha were extracted separately with petroleum ether, ethyl acetate and methanol. Antidermatophytic activity was determined by agar tube dilution method against Epidermophyton floccosum var. nigricans, Microsporum canis, Microsporum gypseum and Trichophyton rubrum. The activities of various parts of the plant - flowers, leaves and stem were separately analyzed using their ethyl acetate extract. Fungicidal efficacy and trypsin inhibiting activity of the whole plant, flowers and leaves were also analyzed using the ethyl acetate extracts.
  
  
  METHODS
  For trypsin inhibition assay results are expressed as mean ± standard division. For antidermatophytic assay, the significance of the difference between control and test was analyzed statistically using Fisher's exact test.
  
  
  RESULTS
  Ethyl acetate extract of M. micrantha exhibited excellent antidermatophytic activity, followed by petroleum ether and methanolic extracts. Ethyl acetate extracts of whole plant, flowers, leaves and stem completely inhibited the growth of dermatophytes at the tested concentration of 2 mg/mL. Furthermore, ethyl acetate extracts of whole plant, leaves and flowers were fungicidal, and the percentages of trypsin inhibition exhibited were 33.73 ± 0.306, 39.0 ± 0.505 and 35.53 ± 0.503, respectively.
  
  
  CONCLUSIONS
  Since M. micrantha possesses antidermatophytic as well as anti-inflammatory activities, the plant is an excellent candidate for the development of new medicaments against dermatophytoses in traditional as well as modern medicine.
authors.affiliation: Biochemistry and Pharmacognosy Research Lab, School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors.forename: Madhavankutty
authors.initials: M
authors.lastname: Jyothilakshmi
authors.affiliation: Microbiology Research Lab, School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors.forename: Mathew
authors.initials: M
authors.lastname: Jyothis
authors.affiliation: Biochemistry and Pharmacognosy Research Lab, School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors.forename: Mukalel Sankunni
authors.initials: MS
authors.lastname: Latha
keywords: Anti-inflammatory action; Epidermophyton; Microsporum; Trichophyton; Trypsin inhibiting assay