title: Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cells in Arabidopsis.
abstract: This study functionally characterizes the Arabidopsis (Arabidopsis thaliana) plastidial glycolytic isoforms of glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in photosynthetic and heterotrophic cells. We expressed the enzyme in gapcp double mutants (gapcp1gapcp2) under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 had no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affected Arabidopsis development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Our results indicate that GAPCp1 is not functionally important in photosynthetic cells but plays a fundamental role in roots and in heterotrophic cells of the AP. Specifically, GAPCp activity may be required in root meristems and the root cap for normal primary root growth. Transcriptomic and metabolomic analyses indicate that the lack of GAPCp activity affects nitrogen and carbon metabolism as well as mineral nutrition and that glycerate and glutamine are the main metabolites responding to GAPCp activity. Thus, GAPCp could be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of γ-aminobutyrate, which in turn affect plant development.
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Armand D
authors.identifier: http://orcid.org/0000-0003-0043-2180
authors.initials: AD
authors.lastname: Anoman
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Jesús
authors.identifier: http://orcid.org/0000-0002-2099-3754
authors.initials: J
authors.lastname: Muñoz-Bertomeu
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Sara
authors.identifier: http://orcid.org/0000-0002-6123-8173
authors.initials: S
authors.lastname: Rosa-Téllez
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: María
authors.identifier: http://orcid.org/0000-0002-9296-0070
authors.initials: M
authors.lastname: Flores-Tornero
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Ramón
authors.initials: R
authors.lastname: Serrano
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Eduardo
authors.identifier: http://orcid.org/0000-0002-0828-121X
authors.initials: E
authors.lastname: Bueso
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.).
authors.forename: Juan
authors.identifier: http://orcid.org/0000-0001-7774-2676
authors.initials: J
authors.lastname: Segura
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmácia (A.D.A., S.R.-T., M.F.-T., J.S., R.R.) and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (A.D.A., S.R.-T., M.F.-T., J.S., R.R.), Universitat de València, 46100 Burjassot, Spain;Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain (J.M.-B., R.S., E.B.); andMax Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (A.R.F.) roc.ros@uv.es.
authors.forename: Roc
authors.identifier: http://orcid.org/0000-0003-4254-8368
authors.initials: R
authors.lastname: Ros
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes; D009584:Nitrogen / Q000378:metabolism*; D011401:Promoter Regions, Genetic
chemical_list: D029681:Arabidopsis Proteins; D007527:Isoenzymes; D002244:Carbon; C585192:GAPC1 protein, Arabidopsis; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D009584:Nitrogen