title: Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein.
abstract: Reticuloendotheliosis virus (REV) causes an immunosuppressive and oncogenic disease in chickens and other birds. In this study, based on an infectious clone of REV, named HLJR0901, a recombinant virus containing the enhanced green fluorescence protein (EGFP) gene was constructed by inserting the EGFP expression cassette downstream of the 3' terminus of the viral env gene. An EGFP-tagged REV that stably expresses EGFP was rescued. This visible recombinant REV could contribute to the further understanding of the molecular mechanism involved in the replication and pathogenicity of REV.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Deng
authors.forename: Feng
authors.initials: F
authors.lastname: Hu
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.forename: Yuping
authors.initials: Y
authors.lastname: Hua
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001717:Birds; D002645:Chickens; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods*; D014773:Virology / Q000379:methods*; D014779:Virus Replication
chemical_list: D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins