title: A near-infrared multifunctional fluorescent probe with an inherent tumor-targeting property for bioimaging.
abstract: A mitochondria-targeting probe, by conjugating a quaternary ammonium cation with glucosamine modified pH-activated cyanine, was designed and synthesized. This probe has excellent selectivity and sensitivity toward pH, stability, cellular membrane permeability and low cytotoxicity. Owing to the acidic feature of tumors and the more negative mitochondrial membrane potential of tumor cells than that of normal cells, this probe can selectively accumulate in tumor cells and light up its fluorescence. It has been successfully applied for in vivo tumor imaging with a high signal-to-noise ratio. Moreover, this multifunctional switchable sensor was also employed for the fluorescent imaging of the fluctuation of intracellular pH in HeLa cells.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China. lgchen@tju.edu.cn yan@tju.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Jin
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Xing
authors.forename: Xilong
authors.initials: X
authors.lastname: Yan
authors.forename: Ligong
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D005944:Glucosamine / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry; D053078:Membrane Potential, Mitochondrial; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D059629:Signal-To-Noise Ratio; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D000644:Quaternary Ammonium Compounds; D005944:Glucosamine