title: The Presence of Histamine and a Histamine Receptor in the Bivalve Mollusc,Crassostrea virginica.
abstract: Histamine, a biogenic amine, is a neurotransmitter in neurons and sensory receptors in invertebrates. Histamine has rarely been reported in bivalves. We used HPLC with pre-column derivatization using 2,3-naphthalenedicarboxaldehyde (NDA) as a fluorescent labeling agent to measure histamine in ganglia, and peripheral tissues of the oysterCrassostrea virginica. We also used Western Blot technique to look for the presence of a histamine receptor in the mantle rim. HPLC results found histamine present in ng amounts in both the cerebral and visceral ganglia, as well as the mantle rim and other peripheral tissues ofC. virginica. The study confirms and quantifies histamine as an endogenous biogenic amine inC. virginicain the nervous system and innervated organs. Western Blot technique also identified a histamine H2-like receptor present in sensory tissue of the oyster's mantle rim.
authors.affiliation: Medgar Evers College, Brooklyn, NY.
authors.forename: Jarreau
authors.initials: J
authors.lastname: Harrison
authors.affiliation: SUNY at Stony Brook University, Stony Brook, NY.
authors.forename: Kisha
authors.initials: K
authors.lastname: LaFleur
authors.forename: Daniel
authors.initials: D
authors.lastname: Mantone
authors.forename: Beatrix
authors.initials: B
authors.lastname: Boisette
authors.forename: Ave
authors.initials: A
authors.lastname: Harris
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Catapane
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Carroll