title: Stem cell-based pulp tissue engineering: variables enrolled in translation from the bench to the bedside, a systematic review of literature.
abstract: Stem cell-based therapy (SC-BT) is emerging as an alternative for endodontic therapies. The interaction between stem cells and scaffolds plays a crucial role in the generation of a 'friendly cell' microenvironment. The aim of this systematic review was to explore techniques applied to regenerate the pulp-dentine complex tissue using SC-BT. An electronic search into the SciVerse Scopus (SS), ISI Web Science (IWS) and Entrez PubMed (EP) using specific keywords was performed. Specific inclusion and exclusion criteria were predetermined. The search yielded papers, out of which full-text papers were included in the final analyses. Data extraction pooled the results in four main topics: (a) influence of the chemical properties of the scaffolds over cell behaviour; (b) influence of the physical characteristics of scaffolds over cell behaviour; (c) strategies applied to improve the stem cell/scaffold interface; and (d) influence of cue microenvironment on stem cell differentiation towards odontoblast-like cells and pulp-like tissue formation. The relationship between the scaffolds, the environment and the growth factors released from dentine are critical for de novo pulp tissue regeneration. The preconditioning of dentine walls with ethylenediaminetetraacetic acid (EDTA) was imperative for successful pulp-dentine complex regeneration. An analyses of the grouped results revealed that pulp regeneration was an attainable goal.
authors.affiliation: Post Graduation Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: M C M
authors.initials: MC
authors.lastname: Conde
authors.affiliation: Post Graduation Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: L A
authors.initials: LA
authors.lastname: Chisini
authors.affiliation: Post Graduation Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.|Post graduation program in Epidemiology, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: F F
authors.initials: FF
authors.lastname: Demarco
authors.affiliation: Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Nör
authors.affiliation: Department of Oral Surgery and Orthopedics, Pediatric Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Casagrande
authors.affiliation: Department of Semiology and Clinics, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: S B C
authors.initials: SB
authors.lastname: Tarquinio
mesh_terms: D003782:Dental Pulp / Q000254:growth & development*; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
keywords: dental pulp; scaffold; stem cell; tissue engineering