title: Prevention of Aflatoxin B₁-Induced DNA Breaks by β-D-Glucan.
abstract: Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B1 (AFB1) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-D-glucan (Glu) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose, we applied the comet assay to groups of animals that were first administered Glu in three doses (100, 400 and 700 mg/kg bw, respectively) and, 20 min later, 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10 and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The data suggested the formation of a supramolecular complex between AFB1 and β-D-glucan.
authors.affiliation: Genetics Laboratory, National School of Biological Sciences, IPN. "Unidad A. López Mateos". Av. Wilfrido Massieu. Zacatenco, México, DF 07738, Mexico. eduardo.madrigal@lycos.com.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Madrigal-Bujaidar
authors.affiliation: Conservation Medicine Laboratory, Superior School of Medicine, IPN. "Unidad Casco de Santo Tomas". Plan de San Luis y Díaz Mirón. México, DF 11340, Mexico. jmorales101@yahoo.com.mx.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Morales-González
authors.affiliation: Institute of Health Sciences, Autonomous University of Hidalgo State, Ex-Hacienda de la Concepción, Tilcuautla, Hidalgo 42160, Mexico. spmtz68@yahoo.com.mx.
authors.forename: Manuel
authors.initials: M
authors.lastname: Sánchez-Gutiérrez
authors.affiliation: Institute of Health Sciences, Autonomous University of Hidalgo State, Ex-Hacienda de la Concepción, Tilcuautla, Hidalgo 42160, Mexico. jizquierdovega@gmail.com.
authors.forename: Jeannett A
authors.initials: JA
authors.lastname: Izquierdo-Vega
authors.affiliation: Organic Chemistry Department, National School of Biological Sciences, IPN. "Unidad Casco de Santo Tomas". Carpio y Plan de Ayala. México, DF 11340, Mexico. areyesarellano@yahoo.com.mx.
authors.forename: Alicia
authors.initials: A
authors.lastname: Reyes-Arellano
authors.affiliation: Genetics Laboratory, National School of Biological Sciences, IPN. "Unidad A. López Mateos". Av. Wilfrido Massieu. Zacatenco, México, DF 07738, Mexico. isela.alvarez@gmail.com.
authors.forename: Isela
authors.initials: I
authors.lastname: Álvarez-González
authors.affiliation: Preclinical Toxicology Laboratory, National School of Biological Sciences, IPN. "Unidad A. López Mateos". Av. Wilfrido Massieu. Zacatenco, México, DF 07738, Mexico. pastenrich@yahoo.com.mx.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pérez-Pasten
authors.affiliation: Conservation Medicine Laboratory, Superior School of Medicine, IPN. "Unidad Casco de Santo Tomas". Plan de San Luis y Díaz Mirón. México, DF 11340, Mexico. eomsmx@yahoo.com.mx.|Institute of Health Sciences, Autonomous University of Hidalgo State, Ex-Hacienda de la Concepción, Tilcuautla, Hidalgo 42160, Mexico. eomsmx@yahoo.com.mx.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Madrigal-Santillán
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology*; D002273:Carcinogens / Q000737:chemistry / Q000633:toxicity*; D020552:Comet Assay; D003460:Crystallization; D053960:DNA Breaks / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D011509:Proteoglycans; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D011509:Proteoglycans; D047071:beta-Glucans; C010770:polysaccharide-K; D016604:Aflatoxin B1
keywords: aflatoxin B1; antigenotoxicity; glucan; mouse hepatocytes