title: Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.
abstract: Stromal fibroblasts are essential for tumor proliferation and invasion. Here we presented a 3-dimensional (3D) microfluidic co-culture device to reconstruct an in vivo-like tumor microenvironment for investigation of the interactions of cancer-associated fibroblasts (CAFs) and bladder cancer cells. With this device, we verified that the cytokines secreted by bladder cancer cells T24 effectively transform the fibroblasts into CAFs. Compared to fibroblasts, the CAFs, which undergo the aerobic glycolysis, showed higher ability to produce lactate and provide energy for bladder cancer cell proliferation and invasion. We also demonstrated that this kind of tumor-promoting effect was associated with the upregulation of monocarboxylate anion transporter 1 (MCT1) and MCT4 expression in CAFs. We concluded that MCT1 and MCT4 are involved in bladder cancer cell proliferation and invasiveness. Moreover, this 3D microfluidic co-culture device allows for the assay to characterize various cellular events in a single device sequentially, facilitating a better understanding of the interactions among heterotypic cells in a sophisticated microenvironment.
authors.affiliation: a Department of Urology, Affiliated Hospital of Qingdao University, Qingdao, China; Key Laboratory of Urinary System Diseases , Qingdao , China.
authors.forename: Haoqing
authors.initials: H
authors.lastname: Shi
authors.affiliation: b Department of Oncology , Affiliated Hospital of Qingdao University , Qingdao , China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Jiang
authors.affiliation: c Medical College of Qingdao University , Qingdao , China.
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.affiliation: a Department of Urology, Affiliated Hospital of Qingdao University, Qingdao, China; Key Laboratory of Urinary System Diseases , Qingdao , China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Cao
authors.affiliation: a Department of Urology, Affiliated Hospital of Qingdao University, Qingdao, China; Key Laboratory of Urinary System Diseases , Qingdao , China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: a Department of Urology, Affiliated Hospital of Qingdao University, Qingdao, China; Key Laboratory of Urinary System Diseases , Qingdao , China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.affiliation: c Medical College of Qingdao University , Qingdao , China.
authors.forename: Youlin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Urology, Affiliated Hospital of Qingdao University, Qingdao, China; Key Laboratory of Urinary System Diseases , Qingdao , China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Sun
authors.affiliation: a Department of Urology, Affiliated Hospital of Qingdao University, Qingdao, China; Key Laboratory of Urinary System Diseases , Qingdao , China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Niu
mesh_terms: D045744:Cell Line, Tumor; D018920:Coculture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology; D006019:Glycolysis / Q000235:genetics / Q000502:physiology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D019344:Lactic Acid / Q000378:metabolism; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D059016:Tumor Microenvironment / Q000235:genetics / Q000502:physiology; D001749:Urinary Bladder Neoplasms / Q000473:pathology; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C406962:SLC16A4 protein, human
keywords: 3D microfluidic device; aerobic glycolysis; bladder cancer; cancer-associated fibroblast; co-culture; invasiveness; lactate; monocarboxylate anion transporter; proliferation