title: Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests.
abstract: Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Heraud
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France. ; Hospices Civils de Lyon, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron, France. ; International Center for Research in Infectiology, INSERM U 1111, Lyon, France. ; Université de Lyon, Faculté de Médecine Lyon Est, Domaine de la Buire, Lyon, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Freydiere
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France. ; Research group on ≪Bacterial Opportunistic Pathogens and Environment≫, UMR 5557 Ecologie Microbienne, CNRS, Université Lyon 1, ENVL, Université de Lyon, Lyon, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Doleans-Jordheim
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France. ; Hospices Civils de Lyon, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron, France. ; International Center for Research in Infectiology, INSERM U 1111, Lyon, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Bes
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France. ; Hospices Civils de Lyon, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron, France. ; International Center for Research in Infectiology, INSERM U 1111, Lyon, France. ; Université de Lyon, Faculté de Médecine Lyon Est, Domaine de la Buire, Lyon, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Tristan
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France. ; Hospices Civils de Lyon, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron, France. ; International Center for Research in Infectiology, INSERM U 1111, Lyon, France. ; Université de Lyon, Faculté de Médecine Lyon Est, Domaine de la Buire, Lyon, France.
authors.forename: François
authors.initials: F
authors.lastname: Vandenesch
authors.affiliation: Hospices Civils de Lyon, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron, France. ; International Center for Research in Infectiology, INSERM U 1111, Lyon, France. ; Université de Lyon, Faculté de Médecine Lyon Est, Domaine de la Buire, Lyon, France. ; Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et Pathologie Nord, Lyon, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Laurent
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron, France. ; Hospices Civils de Lyon, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron, France. ; International Center for Research in Infectiology, INSERM U 1111, Lyon, France. ; Université de Lyon, Faculté de Médecine Lyon Est, Domaine de la Buire, Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Dauwalder
mesh_terms: D006801:Humans; D008712:Methicillin / Q000494:pharmacology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D011933:Reagent Kits, Diagnostic; D008712:Methicillin
keywords: Accuracy; Blood culture; Immunochromatographic test; Methicillin resistance; Staphylococcus aureus