title: Three-Dimensional Bioprinting for Regenerative Dentistry and Craniofacial Tissue Engineering.
abstract: Craniofacial tissues are organized with complex 3-dimensional (3D) architectures. Mimicking such 3D complexity and the multicellular interactions naturally occurring in craniofacial structures represents one of the greatest challenges in regenerative dentistry. Three-dimensional bioprinting of tissues and biological structures has been proposed as a promising alternative to address some of these key challenges. It enables precise manufacture of various biomaterials with complex 3D architectures, while being compatible with multiple cell sources and being customizable to patient-specific needs. This review describes different 3D bioprinting methods and summarizes how different classes of biomaterials (polymer hydrogels, ceramics, composites, and cell aggregates) may be used for 3D biomanufacturing of scaffolds, as well as craniofacial tissue analogs. While the fabrication of scaffolds upon which cells attach, migrate, and proliferate is already in use, printing of all the components that form a tissue (living cells and matrix materials together) to produce tissue constructs is still in its early stages. In summary, this review seeks to highlight some of the key advantages of 3D bioprinting technology for the regeneration of craniofacial structures. Additionally, it stimulates progress on the development of strategies that will promote the translation of craniofacial tissue engineering from the laboratory bench to the chair side.
authors.affiliation: Bioengineering Laboratory, Faculty of Dentistry, University of Sydney, Sydney, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Obregon
authors.affiliation: Institute of Health and Biomedical Innovation, Kelvin Grove, Brisbane, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Vaquette
authors.affiliation: Griffith Health Institute, Regenerative Medicine Center, School of Dentistry and Oral Health, Griffith University, Gold Coast, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Ivanovski
authors.affiliation: Institute of Health and Biomedical Innovation, Kelvin Grove, Brisbane, Australia.
authors.forename: D W
authors.initials: DW
authors.lastname: Hutmacher
authors.affiliation: Bioengineering Laboratory, Faculty of Dentistry, University of Sydney, Sydney, Australia Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, Oregon Health and Science University, Portland, OR, USA bertasso@ohsu.edu.
authors.forename: L E
authors.initials: LE
authors.lastname: Bertassoni
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D001861:Bone Regeneration / Q000502:physiology; D005147:Facial Bones / Q000601:surgery; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D066330:Printing, Three-Dimensional; D012886:Skull / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials
keywords: 3D printing; biofabrication; bone regeneration; craniofacial regeneration; guided tissue regeneration; tissue scaffolds