title: Effects of Atrazine on the Development of Neural System of Zebrafish, Danio rerio.
abstract: By comparative analysis of histomorphology and AChE activity, the changes of physiological and biochemical parameters were determined in zebrafish embryos and larvae dealt with atrazine (ATR) at different concentrations (0.0001, 0.001, 0.01, 0.1, and 1 mg/L). This study showed that the development of the sarcomere and the arrangement of white muscle myofibers were affected by ATR significantly and the length of sarcomere shortened. Further analysis of the results showed that the AChE activity in juvenile fish which was treated with ATR was downregulated, which can indicate that the innervation efficiency to the muscle was impaired. Conversely, the AChE activity in zebrafish embryos which was treated with ATR was upregulated. A parallel phenomenon showed that embryonic primary sensory neurons (Rohon-Beard cells), principally expressing AChE in embryos, survived the physiological apoptosis. These phenomena demonstrated that the motor integration ability of the zebrafish was damaged by ATR which can disturb the development of sensory neurons and sarcomere and the innervations of muscle.
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Mu
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.
authors.forename: Xianjiang
authors.initials: X
authors.lastname: Kang
mesh_terms: D000818:Animals; D001280:Atrazine / Q000008:administration & dosage* / Q000633:toxicity; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects* / Q000276:immunology; D007814:Larva; D012518:Sarcomeres / Q000187:drug effects* / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015027:Zebrafish / Q000196:embryology
chemical_list: D014874:Water Pollutants, Chemical; D001280:Atrazine