title: Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system.
abstract: The clinical application of mesenchymal stromal/stem cells (MSCs) has been extensively explored. In this study, we examined the availability of freshly donated umbilical cord blood (UCB) units that do not qualify for the Japanese banking system for transplantation because of their small volume as a source of MSCs. Forty-five UCB units were used. The median volume of each UCB unit and number of nucleated cells per unit were 40 mL and 5.39 × 10(8), respectively. MSCs were successfully isolated from 18 of 45 units (40 %). The MSC isolation rate was not affected by cell processing method or the interval between delivery and cell processing. The volume of the UCB unit and the mononuclear cell count were predictive factors of the MSC isolation rate. MSCs were effectively isolated by selecting UCB units with a volume of ≥54 mL and containing ≥1.28 × 10(8) mononuclear cells, yielding a MSC isolation rate of >70 %. UCB-derived MSCs were similar to bone marrow-derived MSCs in terms of their morphology, surface marker expression, and differentiation potential, apart from adipogenesis. Our data indicate that UCB units that are currently discarded due to inadequate volume should be reconsidered as a source of MSCs using the well-established UCB banking system.
authors.affiliation: Department of Hematology, Kobe City Medical Center General Hospital, Kobe, 650-0046, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshioka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Miura
authors.forename: Masaki
authors.initials: M
authors.lastname: Iwasa
authors.forename: Aya
authors.initials: A
authors.lastname: Fujishiro
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Yao
authors.forename: Masako
authors.initials: M
authors.lastname: Miura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Fukuoka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Asumi
authors.initials: A
authors.lastname: Yokota
authors.forename: Hideyo
authors.initials: H
authors.lastname: Hirai
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Ichinohe
authors.forename: Akifumi
authors.initials: A
authors.lastname: Takaori-Kondo
authors.forename: Taira
authors.initials: T
authors.lastname: Maekawa
mesh_terms: D018070:Biological Specimen Banks; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D059785:Karyotype; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D012680:Sensitivity and Specificity