title: Protective effects of hydrogen-rich medium on lipopolysaccharide-induced monocytic adhesion and vascular endothelial permeability through regulation of vascular endothelial cadherin.
abstract: We observed the effect of hydrogen-rich medium on lipopolysaccharide (LPS)-induced human umbilical vein endothelial cells (HUVECs), hyaline leukocyte conglutination, and permeability of the endothelium. Endotheliocytes were inoculated on 6-well plates and randomly divided into 4 groups: control, H2, LPS, LPS+H2, H2, and LPS+H2 in saturated hydrogen-rich medium. We applied Wright's stain-ing to observe conglutination of hyaline leukocytes and HUVECs, flow cytometry to determine the content of vascular cell adhesion protein 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1), enzyme-linked immunosorbent assay to measure the E-selectin concentration in the cell liquor, the transendothelial electrical resistance (TEER) to test the permeability of endothelial cells, and Western blot and immunofluorescence to test the expression and distribution of vascular endothelial (VE)-cadherin. Compared with control cells, there was an increase in endothelium-hyaline leukocyte conglutination, a reduction in VCAM-1, ICAM-1, and E-selectin, and the TEER value increased obviously. Compared with LPS, there was an obvious reduction in the conglutination of LPS+H2 cells, a reduction in VCAM-1, ICAM-1, and E-selectin levels, and a reduction in the TEER-resistance value, while the expression of VE-cadherin increased. Fluorescence results showed that, compared with control cells, the VE-cadherin in LPS cells was in-complete at the cell joints. Compared with LPS cells, the VE-cadherin in LPS+H2 cells was even and complete at the cell joints. Liquid rich in hydrogen could reduce LPS-induced production of adhesion molecules and endothelium-hyaline leukocyte conglutination, and influence the expression and distribution of VE-cadherin to regulate the permeability of the endothelium.
authors.affiliation: Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: W N
authors.initials: WN
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Han
authors.affiliation: Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: K L
authors.initials: KL
authors.lastname: Xie
authors.affiliation: Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, China yuhonghao_b@163.com.
authors.forename: Y H
authors.initials: YH
authors.lastname: Yu
mesh_terms: D015820:Cadherins / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006859:Hydrogen / Q000493:pharmacokinetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D015820:Cadherins; D003470:Culture Media; D008070:Lipopolysaccharides; D006859:Hydrogen