title: Faithful expression of imprinted genes in donor cells of SCNT cloned pigs.
abstract: To understand if the genomic imprinting status of the donor cells is altered during the process of SCNT (somatic cell nuclear transfer), cloned pigs were produced by SCNT using PEF (porcine embryonic fibroblast) and P-PEF (parthenogenetic-PEF) cells as donors. Then, the gene expression and methylation patterns of H19, IGF2, NNAT and MEST were compared between PEF vs. C-PEF (cloned-PEF), P-PEF vs. CP-PEF (cloned-P-PEF), respectively. Taken together, the results revealed that there was no significant difference in the expression of imprinted genes and conserved genomic imprints between the donor and cloned cells.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sui
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Song
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors.forename: Anfeng
authors.initials: A
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China. Electronic address: lizj_1998@jlu.edu.cn.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China; Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China. Electronic address: lai_liangxue@gibh.ac.cn.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002681:China; D002999:Clone Cells; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D019175:DNA Methylation; D004477:Ectogenesis; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000166:cytology; D047108:Embryonic Development; D044127:Epigenesis, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D053652:Nuclear Transfer Techniques / Q000009:adverse effects / Q000662:veterinary; D010312:Parthenogenesis; D011247:Pregnancy; D034421:Sus scrofa / Q000235:genetics / Q000378:metabolism*
keywords: Cloned pig; Imprinted gene; Parthenogenetic; Porcine embryonic fibroblast; Somatic-cell nuclear transfer