title: Fatty acids in bacterium Dietzia sp. grown on simple and complex hydrocarbons determined as FAME by GC-MS.
abstract: The influence of growth substrates on the fatty acids produced by Dietzia sp. A14101 has been studied to investigate how qualitative and semi-quantitative information on fatty acids correlates with the ability of this strain to access and utilize a wide range of water-immiscible HC-substrates by modifying the FA content and thus also the properties of the cellular membrane. After incubation on different substrates and media, the profiles of fatty acids (FA) were analyzed by gas chromatography and mass spectrometry (GC-MS). The equivalent chain length (ECL) index calibration system was employed to identify FA. The effect of each substrate on the cell surface charge and on the hydrophobicity of the cellular membrane was also investigated. The results indicate that the variation of the content of saturated fatty acids (SAT-FA) versus mono-unsaturated fatty acids (MUFA) was found to be the most pronounced while branched FA exhibited much less variation in spite of different substrate regimes. The regulation of the ratio of SAT-FA and MUFA seems to be coupled with the regulation of the charge and hydrophobicity of the outer cellular surface. The exposure to a water immiscible substrate led to the development of the negative cellular surface charge, production of carotenoid-type pigments and increased hydrophobicity of the cellular surface. The specific aspects of the adaptation mechanism could have implications for bioremediation and/or (M)EOR applications.
authors.affiliation: Department of Chemistry, University of Bergen, Allégaten 41, 5007 Bergen, Norway. Electronic address: ina.hvidsten@kj.uib.no.
authors.forename: Ina
authors.initials: I
authors.lastname: Hvidsten
authors.affiliation: Department of Chemistry, University of Bergen, Allégaten 41, 5007 Bergen, Norway.
authors.forename: Svein Are
authors.initials: SA
authors.lastname: Mjøs
authors.affiliation: Uni Research CIPR, Uni Research, P.O. Box 7810, 5020 Bergen, Norway.
authors.forename: Gunhild
authors.initials: G
authors.lastname: Bødtker
authors.affiliation: Department of Chemistry, University of Bergen, Allégaten 41, 5007 Bergen, Norway.
authors.forename: Tanja
authors.initials: T
authors.lastname: Barth
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D004952:Esters / Q000032:analysis* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000032:analysis* / Q000378:metabolism
chemical_list: D004952:Esters; D005227:Fatty Acids; D006838:Hydrocarbons
keywords: Bio-surfactants; Fatty acid; Genus dietzia; Hydrocarbon-degradation