title: A specific proteomic response of Sulfolobus solfataricus P2 to gamma radiations.
abstract: Sulfolobus solfataricus is an acidophilic hyperthermophilic crenarchaeon living at 80 °C in aerobic conditions. As other thermophilic organisms, S. solfataricus is resistant to gamma irradiation and we studied the response of this microorganism to this ionizing irradiation by monitoring cell growth, DNA integrity and proteome variations. In aerobic conditions, the S. solfataricus genome was fragmented due to the multiple DNA double strand breakages induced by γ-rays and was fully restored within a couple of hours. Comparison of irradiated and unirradiated cell proteomes indicated that only few proteins changed. The proteins identified by mass spectrometry are involved in different cellular pathways including DNA replication, recombination and repair. Interestingly, we observed that some proteins are irradiation dose-specific while others are common to the cell response regardless of the irradiation dose. Most of the proteins highlighted in these conditions seem to act together to allow an efficient cell response to γ-irradiation.
authors.affiliation: Université Versailles St-Quentin, 45 Avenue des Etats-Unis, 78035 Versailles, France; Université Paris-Sud, Institut de Génétique et Microbiologie, UMR 8621 CNRS, Bât. 409, 91405 Orsay Cedex, France.
authors.forename: Sharon
authors.initials: S
authors.lastname: Larmony
authors.affiliation: Université Versailles St-Quentin, 45 Avenue des Etats-Unis, 78035 Versailles, France; Université Paris-Sud, Institut de Génétique et Microbiologie, UMR 8621 CNRS, Bât. 409, 91405 Orsay Cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Garnier
authors.affiliation: Université Versailles St-Quentin, 45 Avenue des Etats-Unis, 78035 Versailles, France; Université Paris-Sud, Institut de Génétique et Microbiologie, UMR 8621 CNRS, Bât. 409, 91405 Orsay Cedex, France.
authors.forename: Astrid
authors.initials: A
authors.lastname: Hoste
authors.affiliation: Université Versailles St-Quentin, 45 Avenue des Etats-Unis, 78035 Versailles, France; Université Paris-Sud, Institut de Génétique et Microbiologie, UMR 8621 CNRS, Bât. 409, 91405 Orsay Cedex, France. Electronic address: nadal.marc@ijm.univ-paris-diderot.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Nadal
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D004260:DNA Repair / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays / Q000009:adverse effects*; D013058:Mass Spectrometry; D020543:Proteome / Q000528:radiation effects*; D048229:Sulfolobus solfataricus / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D019843:Archaeal Proteins; D020543:Proteome
keywords: DNA repair; Gamma radiation; Hyperthermophilic archaea; Proteomic