title: Target-antigen Detection and Localization of Human Amniotic-derived Cells after in Utero Transplantation in Rats.
abstract: Human amniotic-derived cells (hAMCs) have recently raised interest for their differentiation capability and immunomodulatory properties. To assess the feasibility of hAMCs therapeutic treatment during fetal development, we explored the localization of cells derived from the human amniotic membrane in rat organs after in utero transplantation. Rats were sacrificed at different time points and their organs were analyzed for the distribution of hAMCs by immunohistochemistry using an antibody against Human Cytoplasm and through detection of human DNA. Immunohistochemical and PCR analysis showed that most of the rat tissues presented human cells/DNA suggesting a widespread migration of hAMCs after transplantation. We developed an efficient target-antigen detection method based on an immunohistochemical technique that resulted to be highly specific and sensitive to identify the hAMCs into rat tissues.
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy gburrai@uniss.it.
authors.forename: Giovanni Pietro
authors.initials: GP
authors.lastname: Burrai
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Antuofermo
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy Centro di Ricerca E. Menni, Fondazione Poliambulanza-Istituto Ospedaliero, Brescia, Italy.
authors.forename: Serafina
authors.initials: S
authors.lastname: Farigu
authors.affiliation: Centro di Ricerca E. Menni, Fondazione Poliambulanza-Istituto Ospedaliero, Brescia, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Cargnoni
authors.affiliation: Centro di Ricerca E. Menni, Fondazione Poliambulanza-Istituto Ospedaliero, Brescia, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bonassi
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Pasciu
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy.
authors.forename: Maria Piera
authors.initials: MP
authors.lastname: Demontis
authors.affiliation: Centro di Ricerca E. Menni, Fondazione Poliambulanza-Istituto Ospedaliero, Brescia, Italy.
authors.forename: Ornella
authors.initials: O
authors.lastname: Parolini
authors.affiliation: Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Sassari, Italy.
authors.forename: Maria Vittoria
authors.initials: MV
authors.lastname: Varoni
mesh_terms: D000650:Amnion / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D000941:Antigens / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D017207:Rats, Sprague-Dawley; D016019:Survival Analysis
chemical_list: D000941:Antigens; D004247:DNA
keywords: Human amniotic-derived cells; Immunohistochemistry; In utero transplantation; PCR; Placenta