title: Simultaneous determination of eight chemicals in Fufang Xueshuantong capsules by LC-MS-MS with periodic polarity switching.
abstract: A rapid, sensitive, selective and periodic polarity-switching liquid chromatography-tandem mass spectrometry(LC-MS-MS) method was developed for the simultaneous determination of eight components in an important traditional Chinese medicinal capsule, Fufang Xueshuantong. LC analysis was carried out on a Kinetex 2.6 µm XB-C18 column by gradient elution. The detection was performed by multiple reaction monitoring (MRM) with a periodic polarity-switching electrospray ionization (ESI) source across five periods of switching between positive and negative mode. Periodic polarity-switching was used to acquire maximal responses and to simultaneously detect eight constituents in one analysis run lasting 8 min. All of the analytes showed good linearity (r > 0.9947) in the tested ranges. The average recoveries were in the range of 95.4-103.9% with relative standard deviations ≤6.3%, and the intra- and inter-day variations were ≤3.7%. The developed method was successfully employed to analyze three batches of Fufang Xueshuantong capsule samples. The results showed that the levels of notoginsenoside R1, ginsenoside Rb1 and salvianolic acid B were clearly different in different batches of Fufang Xueshuantong capsules. Furthermore, the new established periodic polarity-switching LC-MS-MS method was proven to be highly sensitive and effective in evaluating the quality of Fufang Xueshuantong capsules.
authors.affiliation: The Second Hospital, Hebei Medical University, Shijiazhuang 050000, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, PR China.
authors.forename: Linfei
authors.initials: L
authors.lastname: Su
authors.affiliation: School of Pharmaceutical Sciences, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, PR China.
authors.forename: Weiding
authors.initials: W
authors.lastname: Shang
authors.affiliation: School of Pharmaceutical Sciences, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, PR China.
authors.forename: Zhihuan
authors.initials: Z
authors.lastname: Rong
authors.affiliation: The Second Hospital, Hebei Medical University, Shijiazhuang 050000, PR China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Sun
authors.affiliation: School of Pharmaceutical Sciences, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, PR China shixiaowei@hebmu.edu.cn.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Sciences, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, PR China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
mesh_terms: D017311:Amlodipine / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D001562:Benzimidazoles / Q000097:blood* / Q000493:pharmacokinetics; D001565:Benzoates / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077333:Telmisartan
chemical_list: D001562:Benzimidazoles; D001565:Benzoates; D017311:Amlodipine; D000077333:Telmisartan