title: PP061. The role of heat shock protein 60 and 70 in early- and late-onset preeclampsia differentiation.
abstract: BACKGROUND
  Preeclampsia is a human pregnancy-specific syndrome characterized by the onset of hypertension and proteinuria. These manifestations may occur before the 34th week of gestation or from this period on, being denominated early-onset or late-onset preeclampsia respectively. The etiology of both disorders seems to differ qualitatively; therefore, different strategies of prevention and treatment must be studied.
  
  
  OBJECTIVE
  The aim of the present study is to determine whether the plasma levels of heat-shock proteins Hsp60 and Hsp70 as well as specific antibodies anti-Hsp60 and anti-Hsp70 may differentiate early-onset from late-onset preeclampsia.
  
  
  METHODS
  We evaluated 175 pregnant women with PE (55 early-onset PE and 120 late-onset PE). Plasma was obtained from peripheral blood and Hsp60, Hsp70 as well as anti-Hsp60 and anti-Hsp70 antibody levels were determined by enzyme immunoassay. Uric acid levels were also determined in the plasma of patients. For statistical analyses, the Mann-Whitney U-test and the Spearman rank order correlation were applied with significance level set at 5%.
  
  
  RESULTS
  Hsp70 levels obtained from early-onset PE group were significantly higher than the late-onset PE women and showed positive correlation with uric acid (r=0.4547; p=0.0028). The Hsp60 production was similar in both groups. Our results also indicate that there was no significant difference of anti-Hsp60 and anti-Hsp70 antibody levels between women with early- and late-onset PE. However,these antibody levels were high,indicating a strong relationship with the production of HSP60 and Hsp70 protein.
  
  
  CONCLUSIONS
  Association between levels of Hsp70 and uric acid in plasma of patients with early-onset PE seems to reflect the oxidative stress in this group of patients. This study provides evidence that Hsp70 determination may be utilized to assess the differentiation between early- and late-onset PE.
  FAPESP 2010/09241-2.
authors.affiliation: Gynecology and Obstetrics, Botucatu, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Peracoli
authors.affiliation: Botucatu Medical School - São Paulo State University, Botucatu, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Bannwart-Castro
authors.affiliation: Microbiology and Immunology, Institute of Biosciences, São Paulo State University, Botucatu, Brazil.
authors.forename: V S I
authors.initials: VS
authors.lastname: Giorgi
authors.affiliation: Gynecology and Obstetrics, Botucatu, Brazil.
authors.forename: I C
authors.initials: IC
authors.lastname: Weel
authors.affiliation: Gynecology and Obstetrics, Botucatu, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Romao
authors.affiliation: Gynecology and Obstetrics, Botucatu, Brazil.
authors.forename: S S
authors.initials: SS
authors.lastname: Witkin
authors.affiliation: Gynecology and Obstetrics, Botucatu, Brazil.
authors.forename: V T M
authors.initials: VT
authors.lastname: Borges
authors.affiliation: Gynecology and Obstetrics, Botucatu, Brazil.
authors.forename: M V C
authors.initials: MV
authors.lastname: Rudge
authors.affiliation: Microbiology and Immunology, Institute of Biosciences, São Paulo State University, Botucatu, Brazil.
authors.forename: M T S
authors.initials: MT
authors.lastname: Peracoli