title: Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47phox Expression in Rats.
abstract: The effect of ellagic acid on oxidative stress and hypertension induced by Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) was investigated. Male Sprague-Dawley rats were administrated with L-NAME (40 mg/kg/day) for five weeks. L-NAME induced high systolic blood pressure (SBP) and increased heart rate (HR), hindlimb vascular resistance (HVR) and oxidative stress. Concurrent treatment with ellagic acid (7.5 or 15 mg/kg) prevented these alterations. Co-treatment with ellagic acid was associated with up-regulation of endothelial nitric oxide synthase (eNOS) protein production and alleviation of oxidative stress as indicated by decreased superoxide production in the vascular tissue, reduced plasma malondialdehyde levels, reduced NADPH oxidase subunit p47phox expression and increased plasma nitrate/nitrite levels. Our results indicate that ellagic acid attenuates hypertension by reducing NADPH oxidase subunit p47phox expression, which prevents oxidative stress and restores NO bioavailability.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. no_ng_pt@hotmail.com.
authors.forename: Thewarid
authors.initials: T
authors.lastname: Berkban
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. ton_pt_kku@hotmail.com.
authors.forename: Pattanapong
authors.initials: P
authors.lastname: Boonprom
authors.affiliation: Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. bugvo@hotmail.com.
authors.forename: Sarawoot
authors.initials: S
authors.lastname: Bunbupha
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. jariya@kku.ac.th.
authors.forename: Jariya Umka
authors.initials: JU
authors.lastname: Welbat
authors.affiliation: Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. upa_ku@kku.ac.th.
authors.forename: Upa
authors.initials: U
authors.lastname: Kukongviriyapan
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. veerapol@kku.ac.th.
authors.forename: Veerapol
authors.initials: V
authors.lastname: Kukongviriyapan
authors.affiliation: Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. ppoung@kku.ac.th.
authors.forename: Poungrat
authors.initials: P
authors.lastname: Pakdeechote
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand. parpra@kku.ac.th.
authors.forename: Parichat
authors.initials: P
authors.lastname: Prachaney
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004610:Ellagic Acid / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D019255:NADPH Oxidases / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D009566:Nitrates; D009573:Nitrites; D004610:Ellagic Acid; D009569:Nitric Oxide; D008315:Malondialdehyde; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D019331:NG-Nitroarginine Methyl Ester
keywords: NADPH  oxidase; antioxidant; ellagic acid; high blood pressure; nitric oxide deficiency