title: Differential Proteomic Analysis of Syncytiotrophoblast Extracellular Vesicles from Early-Onset Severe Preeclampsia, using 8-Plex iTRAQ Labeling Coupled with 2D Nano LC-MS/MS.
abstract: OBJECTIVE
  Previous studies have revealed that the increased shedding of syncytiotrophoblast extracellular vesicles (STBM) may lead to preeclampsia (PE). We aimed to identify the proteins carried by STBM and their potential pathological roles in early-onset severe PE.
  
  
  METHODS
  In this study, we performed a differential proteomic analysis of STBM from early-onset severe PE patients, using iTRAQ isobaric tags and 2D nano LC-MS/MS. STBM were generated by the in vitro explant culture method, and then verified by electron microscopy and western blot analysis.
  
  
  RESULTS
  A total of 18 533 unique peptides and 3 317 proteins were identified, 3 292 proteins were quantified. We identified 194 differentially expressed proteins in STBM from early-onset severe PE patients, 122 proteins were up-regulated and 72 proteins were down-regulated. Further bioinformatics analysis revealed that mitochondrion, transmembrane transport and transmembrane transporter activity were the most abundant categories in gene ontology (GO) annotation. Glycolysis/ gluconeogenesis, citrate cycle, fatty acid elongation, steroid hormone biosynthesis and oxidative phosphorylation were the five significantly represented pathways. Four differentially expressed proteins (siglec-6, calnexin, CD63 and S100-A8) related to inflammation, coagulation or immunoregulation were independently verified using western blot.
  
  
  CONCLUSIONS
  The identification of key proteins carried by STBM may serve not only as a basis for better understanding and further exploring the etiology and pathogenesis of PE, but also as potential biomarkers and in providing targets for future therapy in PE, especially in early-onset severe PE(sPE).
authors.affiliation: Department of Obstetrics and Gynecology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Gu
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Liu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhou
authors.forename: Jiongyu
authors.initials: J
authors.lastname: Hu
authors.forename: Meijia
authors.initials: M
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yilin
authors.initials: Y
authors.lastname: Li
authors.forename: Yingru
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jianxin
authors.initials: J
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D002952:Citric Acid Cycle / Q000235:genetics; D000067128:Extracellular Vesicles / Q000737:chemistry*; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005943:Gluconeogenesis / Q000235:genetics; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D012720:Severity of Illness Index; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology
chemical_list: D005227:Fatty Acids; D026901:Membrane Transport Proteins; D020543:Proteome