title: Antiangiogenic Activity of Acer tegmentosum Maxim Water Extract in Vitro and in Vivo.
abstract: Angiogenesis, the formation of new blood vessels, is critical for tumor growth and metastasis. Notably, tumors themselves can lead to angiogenesis by inducing vascular endothelial growth factor (VEGF), which is one of the most potent angiogenic factors. Inhibition of angiogenesis is currently perceived as one of the most promising strategies for the blockage of tumor growth. In this study, we investigated the effects of Acer tegmentosum maxim water extract (ATME) on angiogenesis and its underlying signal mechanism. We studied the antiangiogenic activity of ATME by using human umbilical vein endothelial cells (HUVECs). ATME strongly inhibited VEGF-induced endothelial cell proliferation, migration, invasion, and tube formation, as well as vessel sprouting in a rat aortic ring sprouting assay. Moreover, we found that the p44/42 mitogen activated protein (MAP) kinase signaling pathway is involved in the inhibition of angiogenesis by ATME. Moreover, when we performed the in vivo matrigel plug assay, VEGF-induced angiogenesis was potently reduced when compared to that for the control group. Taken together, these results suggest that ATME exhibits potent antiangiogenic activity in vivo and in vitro and that these effects are regulated by the extracellular regulated kinase (ERK) pathway.
authors.affiliation: Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab., College of Science and Technology, Yonsei University, Wonju, Korea.
authors.forename: Eok-Cheon
authors.initials: EC
authors.lastname: Kim
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
authors.forename: So Hun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Qingdao Endocrine & Diabetes Hospital, Qingdao, China.
authors.forename: Shan-Ji
authors.initials: SJ
authors.lastname: Piao
authors.affiliation: Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab., College of Science and Technology, Yonsei University, Wonju, Korea.
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
authors.affiliation: Division of Biological Science and Technology and Yonsei-Fraunhofer Medical Device Lab., College of Science and Technology, Yonsei University, Wonju, Korea.
authors.forename: Kiho
authors.initials: K
authors.lastname: Bae
authors.affiliation: Department of Biomedical Engineering, Institute of Medical Engineering and Yonsei-Fraunhofer Medical Device Laboratory, Yonsei University, Wonju, Korea.
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon, Korea.
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Hong
authors.affiliation: Department of Obstetrics & Gynecology, Inha University College of Medicine, Incheon, Korea.
authors.forename: Byoung Ick
authors.initials: BI
authors.lastname: Lee
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea. ; Department of Clinical Pharmacology, Inha University School of Medicine, Incheon, Korea.
authors.forename: Moonsuk
authors.initials: M
authors.lastname: Nam
mesh_terms: D031002:Acer / Q000378:metabolism*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000517:prevention & control; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts; D014157:Transcription Factors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C478359:WDR77 protein, human; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D048049:Extracellular Signal-Regulated MAP Kinases; D019950:Mitogen-Activated Protein Kinase 1
keywords: Acer tegmentosum Maxim; Angiogenesis; Vascular Endothelial Growth Factor A; p44/42 MAP Kinase