title: miR-1322 Binding Sites in Paralogous and Orthologous Genes.
abstract: We searched for 2,563 microRNA (miRNA) binding sites in 17,494 mRNA sequences of human genes. miR-1322 has more than 2,000 binding sites in 1,058 genes with ΔG/ΔG m ratio of 85% and more. miR-1322 has 1,889 binding sites in CDSs, 215 binding sites in 5' UTRs, and 160 binding sites in 3' UTRs. From two to 28 binding sites have arranged localization with the start position through three nucleotides of each following binding site. The nucleotide sequences of these sites in CDSs encode oligopeptides with the same and/or different amino acid sequences. We found that 33% of the target genes encoded transcription factors. miR-1322 has arranged binding sites in the CDSs of orthologous MAMLD1, MAML2, and MAML3 genes. These sites encode a polyglutamine oligopeptide ranging from six to 47 amino acids in length. The properties of miR-1322 binding sites in orthologous and paralogous target genes are discussed.
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.
authors.forename: Raigul
authors.initials: R
authors.lastname: Niyazova
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.
authors.forename: Olga
authors.initials: O
authors.lastname: Berillo
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.
authors.forename: Shara
authors.initials: S
authors.lastname: Atambayeva
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.
authors.forename: Anna
authors.initials: A
authors.lastname: Pyrkova
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.
authors.forename: Aigul
authors.initials: A
authors.lastname: Alybayeva
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.
authors.forename: Anatoly
authors.initials: A
authors.lastname: Ivashchenko
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D001665:Binding Sites / Q000235:genetics*; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C583581:MIRN1322 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger