title: ASSOCIATION BETWEEN AQUEOUS HUMOR CXC MOTIF CHEMOKINE LIGAND 13 LEVELS AND SUBFOVEAL CHOROIDAL THICKNESS IN NORMAL OLDER SUBJECTS.
abstract: OBJECTIVE
  To investigate the association of subfoveal choroidal thickness with intraocular inflammatory cytokines and chemokines.
  
  
  METHODS
  The subjects consisted of 76 eyes of consecutive cataract patients at the Japan Community Health Care Organization Tokyo Shinjuku Medical Center between September 2010 and August 2012. In addition, two autopsy eyes from elderly males were used for immunohistochemical study. Subfoveal choroidal thickness was measured using spectral domain optical coherence tomography before cataract surgery. At the beginning of cataract surgery, a sample of undiluted aqueous humor was manually aspirated and the concentrations of the following cytokines were determined using a multiplex cytokine assay: IP-10, MCP-1, MMP-9, IL-6, IL-10, CXCL1, CXCL12, CXCL13, and CCL11. The association of subfoveal choroidal thickness with intraocular cytokine concentration was analyzed. Expression of CXCL13 was examined in autopsy eyes.
  
  
  RESULTS
  Among these factors, higher concentration of CXCL13 was associated with thicker choroid. In particular, multiple regression analysis showed that the concentration of CXCL13 was associated with subfoveal choroidal thickness (P = 0.001), independently of axial length (P = 0.049). By immunostaining, CXCL13 was clearly detected in choroidal endothelial cells.
  
  
  CONCLUSIONS
  Aqueous humor concentration of CXCL13 is correlated with subfoveal choroidal thickness in normal subjects.
authors.affiliation: *Department of Ophthalmology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo, Japan; †Department of Ophthalmology, Japan Community Health Care Organization Tokyo Shinjuku Medical Center, Tokyo, Japan; and ‡Department of Ophthalmology, Jichi Medical University, Tochigi, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nomura
authors.forename: Hidenori
authors.initials: H
authors.lastname: Takahashi
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Fujino
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kawashima
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yanagi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000378:metabolism*; D058452:Axial Length, Eye; D002386:Cataract / Q000378:metabolism; D054382:Chemokine CXCL13 / Q000378:metabolism*; D002829:Choroid / Q000473:pathology*; D005260:Female; D005584:Fovea Centralis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D041623:Tomography, Optical Coherence
chemical_list: C110663:CXCL13 protein, human; D054382:Chemokine CXCL13