title: 2D light scattering static cytometry for label-free single cell analysis with submicron resolution.
abstract: Conventional optical cytometric techniques usually measure fluorescence or scattering signals at fixed angles from flowing cells in a liquid stream. Here we develop a novel cytometer that employs a scanning optical fiber to illuminate single static cells on a glass slide, which requires neither microfluidic fabrication nor flow control. This static cytometric technique measures two dimensional (2D) light scattering patterns via a small numerical aperture (0.25) microscope objective for label-free single cell analysis. Good agreement is obtained between the yeast cell experimental and Mie theory simulated patterns. It is demonstrated that the static cytometer with a microscope objective of a low resolution around 1.30 μm has the potential to perform high resolution analysis on yeast cells with distributed sizes. The capability of the static cytometer for size determination with submicron resolution is validated via measurements on standard microspheres with mean diameters of 3.87 and 4.19 μm. Our 2D light scattering static cytometric technique may provide an easy-to-use, label-free, and flow-free method for single cell diagnostics.
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Xie
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Department of Medical Genetics, School of Medicine, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Beihua
authors.initials: B
authors.lastname: Kong
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Xuantao
authors.initials: X
authors.lastname: Su
mesh_terms: D002452:Cell Count / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D005453:Fluorescence; D008027:Light; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000379:methods; D008863:Microspheres; D012441:Saccharomyces cerevisiae; D012542:Scattering, Radiation; D059010:Single-Cell Analysis / Q000379:methods
keywords: 2D light scattering; Mie theory; finite-difference time-domain; flow cytometry; label-free; single cell; static cytometry; submicron resolution