title: Hemicyanine dyes derived from 2,3,3-trimethyl-3H-indolium as candidates for non-covalent protein probes.
abstract: Non-covalent interaction of hemicyanine dyes, derivatives of 2,3,3-trimethyl-3H-indolium with bovine serum albumin has been studied by spectral method. For this purpose, three hemicyanine dyes containing N-(2-carboxyethyl)-2,3,3-trimethyl-3H-indolium moiety were synthesized and their UV/Vis and fluorescence spectra, aggregation, photostability and association with bovine serum albumin were studied. The hemicyanine dyes with 2-ethylcarboxylic group was found to interact with bovine serum albumin, which is probably due to negative charge on the dye molecule at the expense of the carboxylic group and the ability to form hydrogen bonds with albumin.
authors.affiliation: UTP, University of Science and Technology, Faculty of Chemical Technology and Engineering, Seminaryjna 3, 85-326 Bydgoszcz, Poland. Electronic address: nina@utp.edu.pl.
authors.forename: Janina
authors.initials: J
authors.lastname: Kabatc
authors.affiliation: UTP, University of Science and Technology, Faculty of Chemical Technology and Engineering, Seminaryjna 3, 85-326 Bydgoszcz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jurek
authors.affiliation: UTP, University of Science and Technology, Faculty of Chemical Technology and Engineering, Seminaryjna 3, 85-326 Bydgoszcz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kostrzewska
authors.affiliation: Jagiellonian University, Faculty of Chemistry, Ingardena 3, 30-060 Cracow, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Orzeł
mesh_terms: D000418:Albumins / Q000737:chemistry; D000818:Animals; D002232:Carbocyanines / Q000032:analysis*; D002264:Carboxylic Acids / Q000737:chemistry; D002417:Cattle; D002855:Chromatography, Thin Layer; D004396:Coloring Agents / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006860:Hydrogen Bonding; D007211:Indoles / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010777:Photochemistry / Q000379:methods; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D000418:Albumins; D002232:Carbocyanines; D002264:Carboxylic Acids; D004396:Coloring Agents; D005456:Fluorescent Dyes; D007211:Indoles; D012997:Solvents; C000601156:tetramethylene hemicyanine; C000657:cyanine dye 1; D012710:Serum Albumin, Bovine
keywords: Aggregation; Hemicyanines; Interaction with bovine serum albumin; Photostability