title: FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis.
abstract: When a tubular structure forms during early embryogenesis, tubular elongation and lumen formation (epithelialization) proceed simultaneously in a spatiotemporally coordinated manner. We here demonstrate, using the Wolffian duct (WD) of early chicken embryos, that this coordination is regulated by the expression of FGF8, which shifts posteriorly during body axis elongation. FGF8 acts as a chemoattractant on the leader cells of the elongating WD and prevents them from epithelialization, whereas static ('rear') cells that receive progressively less FGF8 undergo epithelialization to form a lumen. Thus, FGF8 acts as a binary switch that distinguishes tubular elongation from lumen formation. The posteriorly shifting FGF8 is also known to regulate somite segmentation, suggesting that multiple types of tissue morphogenesis are coordinately regulated by macroscopic changes in body growth.
authors.affiliation: Department of Zoology, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Atsuta
authors.affiliation: Department of Zoology, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Chiyoda-ku, Tokyo 102-0076, Japan yotayota@develop.zool.kyoto-u.ac.jp.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002630:Chemotactic Factors / Q000494:pharmacology; D002642:Chick Embryo; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D008954:Models, Biological; D038081:Organogenesis / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D014928:Wolffian Ducts / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
chemical_list: D002630:Chemotactic Factors; D051524:Fibroblast Growth Factor 8; D048049:Extracellular Signal-Regulated MAP Kinases; D018631:ras Proteins
keywords: Body axis; Chemoattraction; Live imaging; Wolffian duct/nephric duct