title: Anti-tumor effect of Cordyceps militaris in HCV-infected human hepatocarcinoma 7.5 cells.
abstract: Cordyceps extract has been reported to have various pharmacological activities including an anti-cancer effect. We investigated the inhibitory effect of Cordyceps militaris on hepatitis C virus-infected human hepatocarcinoma 7.5 cells (J6/JFH1-huh 7.5 cells). The huh7.5 cells with or without HCV infection were treated with various concentrations of ethanol extract of Cordyceps militaris (CME) for 48 h and the cytotoxicity was measured by CCK-8 assay. Both J6/JFH1-huh7.5 cells and huh7.5 cells were highly susceptible to CME. To examine the molecular mechanisms of the inhibitory effect on huh7.5 cells, the effect of CME on cell apoptosis was measured using flow cytometry and the expressions of p53, Bim, Bax, PARP, (cleaved) caspase-9, and (cleaved) caspase- 3 in huh 7.5 cells were detected by western blot assays. CME significantly increased early apoptosis and up-regulated the expression of Bim, Bax, cleaved PARP, cleaved caspase 9 and cleaved caspase-3. We also found the decrease of HCV Core or NS3 protein by CME in HCV-infected huh 7.5 cells.
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul, 132-714, Republic of Korea.
authors.forename: Seulki
authors.initials: S
authors.lastname: Lee
authors.forename: Hwan Hee
authors.initials: HH
authors.lastname: Lee
authors.forename: Jisung
authors.initials: J
authors.lastname: Kim
authors.forename: Joohee
authors.initials: J
authors.lastname: Jung
authors.forename: Aree
authors.initials: A
authors.lastname: Moon
authors.forename: Choon-Sik
authors.initials: CS
authors.lastname: Jeong
authors.forename: Hyojeung
authors.initials: H
authors.lastname: Kang
authors.forename: Hyosun
authors.initials: H
authors.lastname: Cho
mesh_terms: D000818:Animals; D000898:Antibiosis; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D032661:Cordyceps / Q000737:chemistry*; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D008807:Mice, Inbred BALB C; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000970:Antineoplastic Agents; D053148:Caspase 3; D053453:Caspase 9; D016159:Tumor Suppressor Protein p53; D017361:Viral Nonstructural Proteins; D051028:bcl-2-Associated X Protein; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C495901:TP53 protein, human