title: Rapid and Automated Quantification of Microalgal Lipids on a Spinning Disc.
abstract: We have developed a fully integrated centrifugal microfluidic device for rapid on-site quantification of lipids from microalgal samples. The fully automated serial process involving cell sedimentation and lysis, liquid-liquid extraction, and colorimetric detection of lipid contents was accomplished within 13 min using a lab-on-a-disc. The presented organic solvent-tolerable (for n-hexane, ethanol) microfluidic disc was newly fabricated by combining thermal fusion bonding and carbon dot-based valving techniques. It is expected that this novel platform will possibly contribute toward sustainable biofuel applications by providing a practical solution for on-site monitoring of lipid accumulation in microalgal samples, thus providing imperative contribution toward energy and environmental purposes of centrifugal microfluidic technology.
authors.affiliation: †Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan 689-798, South Korea.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Kim
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Jeong
authors.forename: Bolam
authors.initials: B
authors.lastname: Kim
authors.forename: Dong-Pyo
authors.initials: DP
authors.lastname: Kim
authors.affiliation: †Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan 689-798, South Korea.|⊥Center for Soft and Living Matter, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan 689-798, South Korea.
authors.forename: Yoon-Kyoung
authors.initials: YK
authors.lastname: Cho
mesh_terms: D001331:Automation; D003124:Colorimetry; D008055:Lipids / Q000032:analysis*; D058086:Microalgae / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D008055:Lipids