title: Influence of collagen addition on the thermal and morphological properties of chitosan/xanthan hydrogels.
abstract: This study investigates the collagen influence on thermal and morphological characteristics of chitosan/xanthan hydrogels for potential tissue engineering applications. Anionic collagen was prepared by selective hydrolysis of type I collagen found in bovine tendons. Chitosan was obtained from the partial deacetylation of squid pen β-chitin and xanthan was acquired from Fluka. The hydrogels were obtained in different ratios and were characterized by thermal and morphological analysis. FT-IR suggested only electrostatic interactions between NH3(+) groups of chitosan and COO(-) groups of xanthan and collagen. Thermogravimetric curves showed that hydrogels contain a great amount of water (above 98%) and the presence of collagen does not change this characteristic. Freezing-bound water transition in DSC curves was shifted to higher values due to the increase of water/polymer interaction, mainly when different ratios of chitosan and xanthan were used. SEM images showed sheet-form structures with the presence of collagen promoting an increase in pore size.
authors.affiliation: Programa Interunidades em Bioengenharia (EESC/FMRP/IQSC), Universidade de São Paulo, Brazil.
authors.forename: Marilia M
authors.initials: MM
authors.lastname: Horn
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, USP, Av. Trab. São-carlense, 400, CP 780, CEP 13560-970, São Carlos, SP, Brazil.
authors.forename: Virginia C A
authors.initials: VC
authors.lastname: Martins
authors.affiliation: Programa Interunidades em Bioengenharia (EESC/FMRP/IQSC), Universidade de São Paulo, Brazil; Instituto de Química de São Carlos, Universidade de São Paulo, USP, Av. Trab. São-carlense, 400, CP 780, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: amplepis@iqsc.usp.br.
authors.forename: Ana Maria de Guzzi
authors.initials: AM
authors.lastname: Plepis
mesh_terms: D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013696:Temperature; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D011135:Polysaccharides, Bacterial; D003094:Collagen; D048271:Chitosan; C002563:xanthan gum
keywords: Chitosan; Collagen; Xanthan