title: Microneutralization assay titres correlate with protection against seasonal influenza H1N1 and H3N2 in children.
abstract: Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies against seasonal H1N1 and H3N2 influenza were measured in children and adolescents (n = 656) by MN and hemagglutination inhibition (HAI) assays. Compared to HAI, the MN assay is more sensitive in detecting serum antibodies and estimates of protective effectiveness against PCR-confirmed infection were higher for both subtypes. Given our findings, the MN assay warrants further consideration as a formal tool for the routine evaluation of vaccine-induced antibody responses.
authors.affiliation: McMaster Immunology Research Centre, McMaster University, Hamilton, Ontario, Canada; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; Michael G DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Chris P
authors.initials: CP
authors.lastname: Verschoor
authors.affiliation: Department of Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Pardeep
authors.initials: P
authors.lastname: Singh
authors.affiliation: Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Margaret L
authors.initials: ML
authors.lastname: Russell
authors.affiliation: McMaster Immunology Research Centre, McMaster University, Hamilton, Ontario, Canada; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; Michael G DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Dawn M E
authors.initials: DM
authors.lastname: Bowdish
authors.affiliation: Research Institute of the McGill University Health Center, Montreal, QC, Canada.
authors.forename: Angela
authors.initials: A
authors.lastname: Brewer
authors.affiliation: Research Institute of the McGill University Health Center, Montreal, QC, Canada.
authors.forename: Louis
authors.initials: L
authors.lastname: Cyr
authors.affiliation: Research Institute of the McGill University Health Center, Montreal, QC, Canada.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Ward
authors.affiliation: McMaster Immunology Research Centre, McMaster University, Hamilton, Ontario, Canada; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; Michael G DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada; Department of Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Loeb
mesh_terms: D000293:Adolescent; D000416:Alberta; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype; D053122:Influenza A Virus, H3N2 Subtype; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000276:immunology*; D008350:Manitoba; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D011446:Prospective Studies
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; C483468:hemagglutinin, human influenza A virus