title: Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos.
abstract: Endogenous peroxiredoxin II (PRDX II) protein plays a vital role in early embryonic development. This study assessed the beneficial effects of exogenous PRDX II on bovine embryo development at the cellular and molecular levels. To this end, in vitro maturation (IVM) medium was supplemented with various concentrations of PRDX II (0, 6.25, 12.5, 25, 50, and 100μg/mL). Of these, 12.5μg/mL PRDX II was the most effective and significantly promoted embryonic development. Therefore, this concentration of PRDX II was used in subsequent experiments. The percentage of embryos that developed into Day 8 blastocysts and the total number of cells per blastocyst (38.2% and 150.6±5.1) was higher in the PRDX II-treated group than in the control (26.4% and 128.9±3.9, respectively). Moreover, the percent of TUNEL positive cells was higher (P<0.05) in the control than in the PRDX II-treated. Furthermore, PRDX II added to the IVM media increased mitochondria content in blastocysts and decreased the intracellular ROS levels in oocytes and blastocysts compared with the control (P<0.05). The expression of genes associated with blastocyst quality (CDX2 and IFNτ), antioxidant activity (SOD2), and mitochondrial activity (TFAM) was higher, whereas the expression of a gene involved in the apoptotic pathway (c-FOS) was lower, in the PRDX II-treated than in the control group. In conclusion, supplementation of IVM medium with PRDX II promotes development to the blastocyst stage and improves blastocyst quality through reducing ROS, enhancing embryonic mitochondrial activity, and modulating development- related target genes expression.
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic of Korea; Department of Animal Breeding and Genetics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal 8210, Bangladesh.
authors.forename: Md
authors.initials: M
authors.lastname: Fakruzzaman
authors.affiliation: Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
authors.forename: Nasser
authors.initials: N
authors.lastname: Ghanem
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic of Korea.
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Bang
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic of Korea.
authors.forename: A-Na
authors.initials: AN
authors.lastname: Ha
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic of Korea.
authors.forename: Kyeong-Lim
authors.initials: KL
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Biotechnology, College of Science and Natural Resource, Gyeongnam National University of Science and Technology, Jinju 660-758, Gyeongsangnam-do, Republic of Korea.
authors.forename: Sea-Hwan
authors.initials: SH
authors.lastname: Sohn
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84322-4700, USA.
authors.forename: Zhongde
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic of Korea; Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-do, Republic of Korea. Electronic address: ikong7900@gmail.com.
authors.forename: Il-Keun
authors.initials: IK
authors.lastname: Kong
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002417:Cattle; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D008928:Mitochondria / Q000187:drug effects*; D054464:Peroxiredoxins / Q000494:pharmacology*; D012313:RNA / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017382:Reactive Oxygen Species; D012313:RNA; D054464:Peroxiredoxins
keywords: Apoptosis; Blastocyst; Bovine; Gene expression; Mitochondria; PRDX II