title: Discovery a novel organic solvent tolerant esterase from Salinispora arenicola CNP193 through genome mining.
abstract: An esterase gene, encoding a 325-amino-acid protein (SAestA), was mined form obligate marine actinomycete strain Salinispora arenicola CNP193 genome sequence. Phylogenetic analysis of the deduced amino acid sequence showed that the enzyme belonged to the family IV of lipolytic enzymes. The gene was cloned, expressed in Escherichia coli as a His-tagged protein, purified and characterized. The molecular weight of His-tagged SAestA is ∼38 kDa. SAestA-His6 was active in a temperature (5-40 °C) and pH range (7.0-11.0), and maximal activity was determined at pH 9.0 and 30 °C. The activity was severely inhibited by Hg(2+), Cu(2+), and Zn(2+). In particular, this enzyme showed remarkable stability in presence of organic solvents (25%, v/v) with log P>2.0 even after incubation for 7 days. All these characteristics suggested that SAestA may be a potential candidate for application in industrial processes in aqueous/organic media.
authors.affiliation: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang, 222005, China; Jiangsu Marine Resources Development Research Institute, Lianyungang, 222000, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Yaowei
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang, 222005, China; Jiangsu Marine Resources Development Research Institute, Lianyungang, 222000, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang, 222005, China; Jiangsu Marine Resources Development Research Institute, Lianyungang, 222000, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang, 222005, China; Jiangsu Marine Resources Development Research Institute, Lianyungang, 222000, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Jiao
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D057225:Data Mining; D004795:Enzyme Stability / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D004950:Esterases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016680:Genome, Bacterial / Q000235:genetics; D023281:Genomics; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008848:Micromonosporaceae / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017421:Sequence Analysis; D012997:Solvents / Q000494:pharmacology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D008670:Metals; D009930:Organic Chemicals; D011994:Recombinant Proteins; D012997:Solvents; D004950:Esterases
keywords: Esterase; Genome mining; S. arenicola