title: Determination of Acidity in Donor Milk.
abstract: BACKGROUND
  There is no uniformity among milk banks on milk acceptance criteria. The acidity obtained by the Dornic titration technique is a widely used quality control in donor milk. However, there are no comparative data with other acidity-measuring techniques, such as the pH meter.
  
  
  OBJECTIVE
  The objective of this study was to assess the correlation between the Dornic technique and the pH measure to determine the pH cutoff corresponding to the Dornic degree limit value used as a reference for donor milk quality control.
  
  
  METHODS
  Fifty-two human milk samples were obtained from 48 donors. Acidity was measured using the Dornic method and pH meter in triplicate. Statistical data analysis to estimate significant correlations between variables was carried out. The Dornic acidity value that led to rejecting donor milk was ≥ 8 Dornic degrees (°D).
  
  
  RESULTS
  In the evaluated sample size, Dornic acidity measure and pH values showed a statistically significant negative correlation (τ = -0.780; P = .000). A pH value of 6.57 corresponds to 8°D and of 7.12 to 4°D.
  
  
  CONCLUSIONS
  Donor milk with a pH over 6.57 may be accepted for subsequent processing in the milk bank. Moreover, the pH measurement seems to be more useful due to certain advantages over the Dornic method, such as objectivity, accuracy, standardization, the lack of chemical reagents required, and the fact that it does not destroy the milk sample.
authors.affiliation: 1 Department of Neonatology, Hospital 12 de Octubre, Madrid, Spain.|2 SAMID Network (Spanish Collaborative Maternal and Child Health Research Network), RD12/0026/0007, Spain.
authors.forename: Diana
authors.initials: D
authors.lastname: Escuder-Vieco
authors.affiliation: 1 Department of Neonatology, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Vázquez-Román
authors.affiliation: 3 Environmental Science Degree, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Sánchez-Pallás
authors.affiliation: 1 Department of Neonatology, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: Noelia
authors.initials: N
authors.lastname: Ureta-Velasco
authors.affiliation: 1 Department of Neonatology, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Mosqueda-Peña
authors.affiliation: 1 Department of Neonatology, Hospital 12 de Octubre, Madrid, Spain.|2 SAMID Network (Spanish Collaborative Maternal and Child Health Research Network), RD12/0026/0007, Spain.
authors.forename: Carmen Rosa
authors.initials: CR
authors.lastname: Pallás-Alonso
mesh_terms: D000136:Acid-Base Equilibrium; D001942:Breast Feeding / Q000706:statistics & numerical data / Q000639:trends; D006801:Humans; D017784:Milk Banks / Q000592:standards*; D008895:Milk, Human / Q000737:chemistry*
keywords: acidity; breastfeeding; donor milk; human milk banking; quality control