title: Treatment study of distal femur for parathyroid hormone (1-34) and β-tricalcium phosphate on bone formation in critical size defects in rats.
abstract: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1-34), a surgically implanted synthetic β-tricalcium phosphate bone biomaterial serving as a matrix to support new bone formation. Twelve weeks after bilateral ovariectomy, all rats underwent bone defect in the distal femurs, and β-tricalcium phosphate was implanted into critical sized defects. After defect operation, all animals were randomly divided into four groups and received following subcutaneous injections until death at four and eight weeks: sham rats (group ST); sham rats + parathyroid hormone, 30 µg/kg, three times a week (group SPT); OVX rats (group OT); and OVX rats + parathyroid hormone (group OPT). The distal femurs of rats were harvested for evaluation. The treatment group demonstrating the highest levels of new bone formation was the defects treated with parathyroid hormone as assessed by micro-computed tomography, biomechanical strength, and histological analysis for sham rats. Furthermore, parathyroid hormone showed a stronger effect on accelerating the degradation of β-tricalcium phosphate. Osteoporosis can limit the function of parathyroid hormone and/or β-tricalcium phosphate. The results from our study demonstrate that combination of parathyroid hormone and β-tricalcium phosphate brings better effect to bone tissue repair in non-osteoporosis and/or osteoporosis status.
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Zhou-Shan
authors.initials: ZS
authors.lastname: Tao
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Qiang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Kai-kai
authors.initials: KK
authors.lastname: Tu
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Huang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Hong-ming
authors.initials: HM
authors.lastname: Xu
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Yang-Xun
authors.initials: YX
authors.lastname: Lv
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China tzs19900327@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000293:injuries* / Q000502:physiology / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D010281:Parathyroid Hormone / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D010281:Parathyroid Hormone; C485817:beta-tricalcium phosphate
keywords: Osteoporotic bone; defect healing; micro-CT; parathyroid hormone; β-tricalcium phosphate