title: Gene expression profiles ofBapx1expressing FACS sorted cells from wildtype andBapx1-EGFPnull mouse embryos.
abstract: The data described in this article refers to Chatterjee et al. (2015) "In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes forBapx1and its co-regulation withSox9in the mammalian vertebral column" (GEO GSE35649) [1]. Transcriptional profiling combined with genome wide binding data is a powerful tool to elucidate the molecular mechanism behind vertebrate organogenesis. It also helps to uncover multiple roles of a single gene in different organs. In the above mentioned report we reveal the function of the homeobox geneBapx1during the embryogenesis of five distinct organs (vertebral column, spleen, gut, forelimb and hindlimb) at a relevant developmental stage (E12.5), microarray analysis of isolated wildtype and mutant cells in is compared in conjunction with ChIP-Seq analysis. We also analyzed the development of the vertebral column by comparing microarray and ChIP-Seq data forBapx1with similarly generated data sets forSox9to generate a gene regulatory network controlling various facets of the organogenesis.
authors.affiliation: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733 N. Broadway, Baltimore, MD 21205 USA.
authors.forename: Sumantra
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
authors.forename: V
authors.initials: V
authors.lastname: Sivakamasundari
authors.affiliation: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Kraus
authors.affiliation: Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
authors.forename: Sook Peng
authors.initials: SP
authors.lastname: Yap
authors.affiliation: Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
authors.forename: Vibhor
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
authors.forename: Shyam
authors.initials: S
authors.lastname: Prabhakar
authors.affiliation: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lufkin
keywords: Bapx1; ChIP-Seq; FACS; Mouse