title: Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei.
abstract: Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or non-endemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas.
authors.forename: Vichaya
authors.initials: V
authors.lastname: Suttisunhakul
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.forename: Chanthiwa
authors.initials: C
authors.lastname: Wikraiphat
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Zakiya
authors.initials: Z
authors.lastname: Douglas
authors.forename: Nicholas P J
authors.initials: NPJ
authors.lastname: Day
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Brett
authors.forename: Mary N
authors.initials: MN
authors.lastname: Burtnick
mesh_terms: D000086:Acetazolamide; D000907:Antibodies, Bacterial / Q000276:immunology*; D016667:Bacterial Capsules / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000276:immunology*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D008554:Melioidosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D008863:Microspheres; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial; D000086:Acetazolamide