title: Effect of leukocyte filtration on the P-selectin expression of apheresis platelets.
abstract: The aim of this study was to investigate the effect of leukocyte filtration on the P-selectin (CD62P) surface expression of apheresis platelets during the retention period. Ten bags of apheresis platelets stored for 1 day (0-24 h) and 10 bags of apheresis platelets stored for 2 days (24-48 h) were used for leukocyte filtration (experimental group). Ten bags of apheresis platelets with the corresponding retention periods but without filtration were used as a negative control (control group). Thereafter, 100 μL of platelet suspensions from apheresis platelets with or without leukocyte filtration were sampled before and after leukocyte filtration for the detection of CD62P surface expression by flow cytometry. No statistical difference in the CD62P surface expression of apheresis platelets was observed before and after leukocyte filtration (P > 0.05), neither did the CD62P surface expression exhibit any change among the different retention periods. Leukocyte filtration does not affect the CD62P surface expression of apheresis platelets stored for up to 2 days, which indicates that leukocyte filtration does not damage the activation of apheresis platelets within the retention period.
authors.affiliation: Department of Blood Transfusion, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China zuotingxie@163.com.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Xie
authors.affiliation: Department of Laboratory, The Third People's Hospital of Cangnan, Wenzhou, China.
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Medical Science, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Zhang
authors.affiliation: Department of Blood Transfusion, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Yang
authors.affiliation: Department of Laboratory, The Second Hospital of Zhejiang University School of Medicine, Hanzhou, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Tao
mesh_terms: D001781:Blood Component Removal; D001792:Blood Platelets / Q000378:metabolism*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D019007:P-Selectin / Q000096:biosynthesis*; D010983:Plateletpheresis / Q000379:methods
chemical_list: D019007:P-Selectin