title: Interfacial Polymerization of Dopamine in a Pickering Emulsion: Synthesis of Cross-Linkable Colloidosomes and Enzyme Immobilization at Oil/Water Interfaces.
abstract: Colloidosomes are promising carriers for immobilizing enzyme for catalytic purposes in aqueous/organic media. However, they often suffer from one or more problems regarding catalytic performance, stability, and recyclability. Here, we report a novel approach for the synthesis of cross-linkable colloidosomes by the selective polymerization of dopamine at oil/water interfaces in a Pickering emulsion. An efficient enzyme immobilization method was further developed by covalently bonding enzymes to the polydopamine (PDA) layer along with the formation of such colloidosomes with lipase as a model enzyme. In this enzyme system, the PDA layer served as a cross-linking layer and enzyme support for simultaneously enhancing the colloidosomes' stability and improving surface availability of the enzymes for catalytic reaction. It was found that the specific activity of lipases immobilized on the colloidosome shells was 8 and 1.4 times higher than that of free lipase and encapsulated lipase positioned in the aqueous cores of colloidosomes, respectively. Moreover, the immobilized lipases demonstrated excellent operational stability and recyclability, retaining 86.6% of enzyme activity after 15 cycles. It is therefore reasonable to expect that this novel approach for enzyme immobilization has great potential to serve as an important technique for the construction of biocatalytic systems.
authors.affiliation: †State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Qu
authors.affiliation: ‡Tianjin Engineering Center of Biomass-derived Gas/Oil Technology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Renliang
authors.initials: R
authors.lastname: Huang
authors.affiliation: †State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.|§Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.|∥Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: †State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.|§Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.|∥Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Rongxin
authors.initials: R
authors.lastname: Su
authors.affiliation: †State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry; D004058:Diffusion; D004298:Dopamine / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008422:Materials Testing; D009821:Oils / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D004655:Emulsions; D004800:Enzymes, Immobilized; D008081:Liposomes; D009821:Oils; D011108:Polymers; D014867:Water; D008049:Lipase; D004298:Dopamine
keywords: Pickering emulsion; colloidosomes; enzyme immobilization; poly(dopamine); silica nanoparticles