title: In Vitro Studies of Bacterial Cellulose and Magnetic Nanoparticles Smart Nanocomposites for Efficient Chronic Wounds Healing.
abstract: The quality of life of patients with chronic wounds can be extremely poor and, therefore, over the past decades, great efforts have been made to develop efficient strategies to improve the healing process and the social impact associated with these conditions. Cell based therapy, as a modern tissue engineering strategy, involves the design of 3D cell-scaffold bioconstructs obtained by preseeding drug loaded scaffolds with undifferentiated cells in order to achieve in situ functional de novo tissue. This paper reports on the development of bionanocomposites based on bacterial cellulose and magnetic nanoparticles (magnetite) for efficient chronic wounds healing. Composites were obtained directly in the cellulose bacterial culture medium by dispersing various amounts of magnetite nanoparticles during the biosynthesis process. After purification and drying, the membranes were characterized by Raman spectroscopy and X-ray diffraction to reveal the presence of magnetite within the bacterial cellulose matrix. Morphological investigation was employed through SEM and TEM analyses on bionanocomposites. The biocompatibility of these innovative materials was studied in relation to human adipose derived stem cells in terms of cellular morphology, viability, and proliferation as well as scaffolds cytotoxic potential.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania ; Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania.
authors.forename: Bianca
authors.identifier: 0000-0001-8093-1750
authors.initials: B
authors.lastname: Galateanu
authors.affiliation: Advanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
authors.forename: Mihaela-Cristina
authors.initials: MC
authors.lastname: Bunea
authors.affiliation: Advanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
authors.forename: Paul
authors.initials: P
authors.lastname: Stanescu
authors.affiliation: Department of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
authors.forename: Eugenia
authors.identifier: 0000-0002-5014-3953
authors.initials: E
authors.lastname: Vasile
authors.affiliation: National Institute for Chemical Pharmaceutical Research and Development, 112 Calea Vitan, 031299 Bucharest, Romania.
authors.forename: Angela
authors.initials: A
authors.lastname: Casarica
authors.affiliation: Advanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
authors.forename: Horia
authors.initials: H
authors.lastname: Iovu
authors.affiliation: Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania.
authors.forename: Anca
authors.identifier: 0000-0001-8510-6653
authors.initials: A
authors.lastname: Hermenean
authors.affiliation: Advanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
authors.forename: Catalin
authors.initials: C
authors.lastname: Zaharia
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania.
authors.forename: Marieta
authors.identifier: 0000-0002-8103-3693
authors.initials: M
authors.lastname: Costache