title: Immunohistochemical Study of Nrf2-Antioxidant Response Element as Indicator of Oxidative Stress Induced by Cadmium in Developing Rats.
abstract: In developing animals, Cadmium (Cd) induces toxicity to many organs including brain. Reactive oxygen species (ROS) are often implicated in Cd-inducedtoxicity and it has been clearly demonstrated that oxidative stress interferes with the expression of genes as well as transcriptional factors such as Nrf2-dependent Antioxidant Response Element (Nrf2-ARE). Cd-generated oxidative stress and elevated Nrf2 activity have been reported in vitro and in situ cells. In this study we evaluated the morphological changes and the expression pattern of Nrf2 and correlated them with the Cd concentrations in different ages of developing rats in heart, lung, kidney, liver, and brain. The Cd content in different organs of rats treated with the metal was increased in all ages assayed. Comparatively, lower Cd brain levels were found in rats intoxicated at the age of 12 days, then pups treated at 5, 10, or 15 days old, at the same metal dose. No evident changes, as a consequence of cadmium exposure, were evident in the morphological analysis in any of the ages assayed. However, Nrf2-ARE immunoreactivity was observed in 15-day-old rats exposed to Cd. Our results support that fully developed blood-brain barrier is an important protector against Cd entrance to brain and that Nrf2 increased expression is a part of protective mechanism against cadmium-induced toxicity.
authors.affiliation: Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Sergio
authors.initials: S
authors.lastname: Montes
authors.affiliation: Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Juárez-Rebollar
authors.affiliation: Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Concepción
authors.initials: C
authors.lastname: Nava-Ruíz
authors.affiliation: Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Aurora
authors.initials: A
authors.lastname: Sánchez-García
authors.affiliation: Departamento de Bioterio, Experimental, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Yesica
authors.initials: Y
authors.lastname: Heras-Romero
authors.affiliation: Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Camilo
authors.initials: C
authors.lastname: Rios
authors.affiliation: Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Insurgentes Sur 3877, La Fama, 14269 Tlalpan, DF, Mexico.
authors.forename: Marisela
authors.initials: M
authors.lastname: Méndez-Armenta
mesh_terms: D000818:Animals; D061745:Antioxidant Response Elements / Q000187:drug effects; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D002104:Cadmium