title: Phytoremediation potential of Arabidopsis with reference to acrylamide and microarray analysis of acrylamide-response genes.
abstract: Acrylamide (ACR) is a widely used industrial chemical. However, it is a dangerous compound because it showed neurotoxic effects in humans and act as reproductive toxicant and carcinogen in many animal species. In the environment, acrylamide has high soil mobility and may travel via groundwater. Phytoremediation is an effective method to remove the environmental pollutants, but the mechanism of plant response to acrylamide remains unknown. With the purpose of assessing remediation potentials of plants for acrylamide, we have examined acrylamide uptake by the model plant Arabidopsis grown on contaminated substrates with high performance liquid chromatography (HPLC) analysis. The result revealed that acrylamide could be absorbed and degraded by Arabidopsis. Further microarray analysis showed that 527 transcripts were up-regulated within 2-days under acrylamide exposure condition. We have found many potential acrylamide-induced genes playing a major role in plant metabolism and phytoremediation.
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Jian-Jie
authors.initials: JJ
authors.lastname: Gao
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Sun
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China. Electronic address: yaoquanhong_sh@aliyun.com.
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
mesh_terms: D020106:Acrylamide / Q000737:chemistry / Q000633:toxicity*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002273:Carcinogens / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant; D046228:Microarray Analysis / Q000379:methods*; D008954:Models, Biological; D015203:Reproducibility of Results; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D002273:Carcinogens; D004785:Environmental Pollutants; D012987:Soil; D020106:Acrylamide
keywords: Acrylamide; Arabidopsis; Microarray; Phytoremediation