title: A novel OFET-based biosensor for the selective and sensitive detection of lactate levels.
abstract: Biosensors based on organic field effect transistors (OFETs) are one of the more promising device applications in organic electronics. However, OFET-based biosensors are still in their early stages of development compared to other electrochemical biosensors. This study is the first to report on an extended-gate type organic field effect transistor (OFET) for lactate detection in aqueous media. Here, the extended-gate electrode of the OFET was modified with layers of a lactate oxidase and a horseradish peroxidase osmium-redox polymer on a flexible plastic film substrate for an enzymatic redox reaction of lactate. The device exhibited both high selectivity and sensitivity. The limit of detection (LOD) and the limit of quantification (LOQ) were estimated to be 66 nM and 220 nM, respectively, which are the sufficient detection limit for practical sensor applications. The obtained results confirm that extended-gate type OFET devices are applicable to enzyme-based biosensors for detecting lactate levels.
authors.affiliation: Research Center for Organic Electronics (ROEL), Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. Electronic address: tminami@yz.yamagata-u.ac.jp.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Minami
authors.affiliation: Research Center for Organic Electronics (ROEL), Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Sato
authors.affiliation: Research Center for Organic Electronics (ROEL), Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Tsukuru
authors.initials: T
authors.lastname: Minamiki
authors.affiliation: Research Center for Organic Electronics (ROEL), Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Fukuda
authors.affiliation: Research Center for Organic Electronics (ROEL), Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kumaki
authors.affiliation: Research Center for Organic Electronics (ROEL), Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Tokito
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D007773:Lactates / Q000032:analysis*; D057230:Limit of Detection; D006899:Mixed Function Oxygenases / Q000737:chemistry; D009992:Osmium / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014173:Transistors, Electronic; D014867:Water / Q000032:analysis
chemical_list: D004800:Enzymes, Immobilized; D007773:Lactates; D011108:Polymers; D014867:Water; D009992:Osmium; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase
keywords: HRP; Lactate; Lactate oxidase; OFET; Organic field effect transistor; Osmium polymer