title: Effects of Caffeine Supplementation on Plasma and Blood Mononuclear Cell Interleukin-10 Levels After Exercise.
abstract: This study compared the response of interleukin (IL)-10, and also of IL-6 and IL-12 p40, to exercise and caffeine supplementation between plasma and blood mononuclear cells (BMNCs). Participants in the study (n = 28) were randomly allocated in a double-blind fashion to either caffeine (n = 14) or placebo (n = 14) treatments. One hour before completing a 15-km run competition, athletes took 6 mg/kg body mass of caffeine or a placebo. Plasma and BMNCs were purified from blood samples taken before and after competition. Concentrations of interleukins (IL-10, IL-6, and IL-12 p40), cyclic adenosine monophosphate (cAMP), caffeine, adrenaline, and cortisol were measured in plasma. IL-10, IL-6, and IL-12 p40 and cAMP levels were also determined in BMNCs. Exercise induced significant increases in IL-6 and IL-10 plasma levels, with higher increases in the caffeine-supplemented group. After 2-hr recovery, these levels returned to almost preexercise values. However, no effect of caffeine on BMNC cytokines was observed. IL-10, IL-6, and IL-12 p40 levels in BMNCs increased mainly at 2 hr postexercise. cAMP levels increased postexercise in plasma and after recovery in BMNCs, but no effects of caffeine were observed. In conclusion, caffeine did not modify cytokine levels in BMNCs in response to exercise. However, higher increases of IL-10 were observed in plasma after exercise in the supplemented participants, which could suppose an enhancement of the anti-inflammatory properties of exercise.
authors.affiliation: Research Group on Evidence, Lifestyles & Health, Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences, University of the Balearic Islands, Palma de Mallorca. Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Tauler
authors.forename: Sonia
authors.initials: S
authors.lastname: Martinez
authors.forename: Pau
authors.initials: P
authors.lastname: Martinez
authors.forename: Leticia
authors.initials: L
authors.lastname: Lozano
authors.forename: Carlos
authors.initials: C
authors.lastname: Moreno
authors.forename: Antoni
authors.initials: A
authors.lastname: Aguiló
mesh_terms: D000328:Adult; D002110:Caffeine / Q000008:administration & dosage* / Q000097:blood; D000242:Cyclic AMP / Q000378:metabolism; D019587:Dietary Supplements; D004311:Double-Blind Method; D004837:Epinephrine / Q000097:blood; D015444:Exercise / Q000502:physiology*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D016753:Interleukin-10 / Q000097:blood*; D053762:Interleukin-12 Subunit p40 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D064133:Sports Nutritional Physiological Phenomena
chemical_list: C508609:IL10 protein, human; C508600:IL6 protein, human; D053762:Interleukin-12 Subunit p40; D015850:Interleukin-6; D016753:Interleukin-10; D002110:Caffeine; D000242:Cyclic AMP; D006854:Hydrocortisone; D004837:Epinephrine