title: [Cytotoxity of pomalidomide combined CAR-T cell for multiple myeloma cell RPMI8226 and U266].
abstract: OBJECTIVE
  To observe the cytotoxity of CD138-CAR-T cells on human multiple myeloma cell RPMI8226 and U266 cells and explore the impact of pomalidomide on the cytotoxity of CD138-CAR-T on RPMI8226 and U266 cells.
  
  
  METHODS
  The cytotoxity of CD138-CAR-T and CD138-CAR-T combined pomalidomide on RPMI8226 and U266 was detected by CFSE/7AAD. The effctor cells were co-cultured with target cells at 5:1 for 18 h, and then the supernatant were collected and used for ELISA assays.
  
  
  RESULTS
  After 18 h co-culture, the cytotoxity of CD138-CAR-T on RPMI8226 and U266 was significantiy higher than control (P<0.01). There was no significant change on the cytotoxity of pomalidoide combined with CD138-CAR-T on RPMI8226 and U266. The results showed that co-cultured system contribted to a markedly increased production of IFN-γ, after adding pomalidomide to the co-cultured system. It can significantly enhance the production of IFN-γ, compared with CD138-CAR-T alone.
  
  
  CONCLUSIONS
  CD138-CAR-T had significantly cytotoxity on U266 and RPMI8226. Pomalidomide could promote CD138-CAR-T cells IFN-γ production.
authors.affiliation: Department of Hematology, Center of Hematologic Diseases of Chinese PLA; Lanzhou Military Area General Hospital, Lanzhou　730050, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Hematology, Center of Hematologic Diseases of Chinese PLA; Lanzhou Military Area General Hospital, Lanzhou　730050, China.
authors.forename: Shuting
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Center of Hematologic Diseases of Chinese PLA; Lanzhou Military Area General Hospital, Lanzhou　730050, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Ou
authors.affiliation: Department of Hematology, Center of Hematologic Diseases of Chinese PLA; Lanzhou Military Area General Hospital, Lanzhou　730050, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Bai
mesh_terms: D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009101:Multiple Myeloma; D051603:Recoverin; D013601:T-Lymphocytes; D013792:Thalidomide / Q000031:analogs & derivatives
chemical_list: C496731:RCVRN protein, human; D051603:Recoverin; D013792:Thalidomide; C467566:pomalidomide