title: Effect of mevalonic acid on cholesterol synthesis in bovine intramuscular and subcutaneous adipocytes.
abstract: Mevalonic acid (MVA) is a key material in the synthesis of cholesterol; indeed, intracellular cholesterol synthesis is also called the mevalonic acid pathway. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. This study suggests that MVA may play an important role in the differentiation of bovine adipose tissue in vivo. We investigated differential mRNA expression in bovine intramuscular preadipocytes (BIPs) and bovine subcutaneous preadipocytes (BSPs) by culturing cells from the longissimus dorsi muscle and subcutaneous fat tissues of Luxi yellow cattle. The morphology of lipid accumulation of bovine preadipocytes was detected by Oil Red O staining, and total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC) levels were measured. Temporospatial expression of HMGR was investigated by real-time quantitative polymerase chain reaction (PCR). The TC, LDLC, and HDLC content did not significantly differ over time but increased slowly with increasing MVA concentration. HMGR expression increased over time and with increasing concentrations of MVA. MVA increased adipose cell proliferation in a dose-dependent and time-dependent manner. MVA stimulated HMGR expression in two cell types and its influence on adipocyte differentiation.
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China.
authors.forename: Xiaomu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: You
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Animal Science, Heze University, Heze, 274015, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China.
authors.forename: Enliang
authors.initials: E
authors.lastname: Song
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China.
authors.forename: Fachun
authors.initials: F
authors.lastname: Wan
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Han
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji'nan City, Shandong Province, 250100, China. liuguifen126@126.com.|Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China. liuguifen126@126.com.
authors.forename: Guifen
authors.initials: G
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D008798:Mevalonic Acid / Q000494:pharmacology*; D009132:Muscles / Q000166:cytology; D050151:Subcutaneous Fat / Q000166:cytology
chemical_list: D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D008798:Mevalonic Acid
keywords: 3-Hydroxy-3-methylglutaryl-CoA reductase; Bovine adipocytes; Cholesterol; Meat quality; Mevalonic acid