title: Effects of Different Maturation Systems on Bovine Oocyte Quality, Plasma Membrane Phospholipid Composition and Resistance to Vitrification and Warming.
abstract: The objective of this study was to evaluate the effects of different maturation systems on oocyte resistance after vitrification and on the phospholipid profile of the oocyte plasma membrane (PM). Four different maturation systems were tested: 1) in vitro maturation using immature oocytes aspirated from slaughterhouse ovaries (CONT; n = 136); 2) in vitro maturation using immature oocytes obtained by ovum pick-up (OPU) from unstimulated heifers (IMA; n = 433); 3) in vitro maturation using immature oocytes obtained by OPU from stimulated heifers (FSH; n = 444); and 4) in vivo maturation using oocytes obtained from heifers stimulated 24 hours prior by an injection of GnRH (MII; n = 658). A sample of matured oocytes from each fresh group was analyzed by matrix associated laser desorption-ionization (MALDI-TOF) to determine their PM composition. Then, half of the matured oocytes from each group were vitrified/warmed (CONT VIT, IMA VIT, FSH VIT and MII VIT), while the other half were used as fresh controls. Afterwards, the eight groups underwent IVF and IVC, and blastocyst development was assessed at D2, D7 and D8. A chi-square test was used to compare embryo development between the groups. Corresponding phospholipid ion intensity was expressed in arbitrary units, and following principal components analyses (PCA) the data were distributed on a 3D graph. Oocytes obtained from superstimulated animals showed a greater rate of developmental (P<0.05) at D7 (MII = 62.4±17.5% and FSH = 58.8±16.1%) compared to those obtained from unstimulated animals (CONT = 37.9±8.5% and IMA = 50.6±14.4%). However, the maturation system did not affect the resistance of oocytes to vitrification because the blastocyst rate at D7 was similar (P>0.05) for all groups (CONT VIT = 2.8±3.5%, IMA VIT = 2.9±4.0%, FSH VIT = 4.3±7.2% and MII VIT = 3.6±7.2%). MALDI-TOF revealed that oocytes from all maturation groups had similar phospholipid contents, except for 760.6 ([PC (34:1) + H]+), which was more highly expressed in MII compared to FSH (P<0.05). The results suggest that although maturation systems improve embryonic development, they do not change the PM composition nor the resistance of bovine oocytes to vitrification.
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
authors.forename: José F W
authors.initials: JF
authors.lastname: Sprícigo
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
authors.forename: Mateus N
authors.initials: MN
authors.lastname: Diógenes
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
authors.forename: Ligiane O
authors.initials: LO
authors.lastname: Leme
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
authors.forename: Carolle V
authors.initials: CV
authors.lastname: Muterlle
authors.affiliation: Embrapa Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Brasília- DF, Brazil.
authors.forename: Bianca Damiani Marques
authors.initials: BD
authors.lastname: Silva
authors.affiliation: Servei de Nutrició i Benestar Animal (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Solà-Oriol
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil.
authors.forename: Ivo
authors.initials: I
authors.lastname: Pivato
authors.affiliation: Embrapa Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Brasília- DF, Brazil.
authors.forename: Luciano Paulino
authors.initials: LP
authors.lastname: Silva
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília-DF, Brazil; Embrapa Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Brasília- DF, Brazil.
authors.forename: Margot A N
authors.initials: MA
authors.lastname: Dode
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D009865:Oocytes / Q000166:cytology*; D010743:Phospholipids / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010743:Phospholipids