title: Proteomic analysis of white and yellow seminal plasma in turkeys (Meleagris gallopavo).
abstract: Yellow semen syndrome (YSS) is endemic within domestic turkey populations. Yellow semen is of lower quality and, when used for insemination, results in reduced fertility and hatchability. Little is known about the etiology of YSS. The aim of this study was to compare the proteome of white and yellow seminal plasma of turkeys using 1) 2-dimensional difference gel electrophoresis (2D-DIGE) to quantify seminal plasma proteins and 2) matrix-assisted laser desorption/ionization mass spectrometry to identify the proteins that are differentially abundant in white and yellow seminal plasma. A total of 49 protein spots (30 upregulated and 19 downregulated) were differentially expressed in yellow seminal plasma compared with white seminal plasma. Transthyretin and serum albumin-like showed a 3-fold increase in seminal plasma from males with YSS, and the latter was validated using Western blot analysis. A 3-fold increase was observed for hemopexin-like and immunoglobulin light chain V-J-C region. Pantetheinase-like showed a 1.3-fold increase. Ovotransferrin, hepatocyte growth factor activator, cysteine-rich secretory protein 3-like, and ferritin heavy chain-like showed a significant decrease (at least a 1.3-fold decrease) in yellow semen. Further studies are necessary to evaluate the precise function of the above-mentioned proteins in YSS and to establish quality markers of turkey semen to predict the reproductive potential of individual turkeys.
authors.forename: M
authors.initials: M
authors.lastname: Słowińska
authors.forename: K
authors.initials: K
authors.lastname: Kozłowski
authors.forename: J
authors.initials: J
authors.lastname: Jankowski
authors.forename: A
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D050439:Genes, Immunoglobulin Light Chain / Q000235:genetics; D008297:Male; D011201:Poultry Diseases / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis / Q000662:veterinary*; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D014422:Turkeys / Q000235:genetics / Q000378:metabolism*
chemical_list: D029589:Seminal Plasma Proteins; D012709:Serum Albumin; D001052:Apoferritins