title: Electron spin resonance of spin-labeled lipid assemblies and proteins.
abstract: Spin-label electron spin resonance (ESR) spectroscopy is a valuable means to study molecular mobility and interactions in biological systems. This paper deals with conventional, continuous wave ESR of nitroxide spin-labels at 9-GHz providing an introduction to the basic principles of the technique and applications to self-assembled lipid aggregates and proteins. Emphasis is given to segmental lipid chain order and rotational dynamics of lipid structures, environmental polarity of membranes and proteins, structure and conformational dynamics of proteins.
authors.affiliation: Department of Physics, University of Calabria, 87036 Rende (CS), Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Guzzi
authors.affiliation: Department of Physics, University of Calabria, 87036 Rende (CS), Italy. Electronic address: rosa.bartucci@fis.unical.it.
authors.forename: Rosa
authors.initials: R
authors.lastname: Bartucci
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D013113:Spin Labels; D013194:Staining and Labeling / Q000379:methods
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D009589:Nitrogen Oxides; D010713:Phosphatidylcholines; D013113:Spin Labels; C039900:nitroxyl
keywords: ESR; Membrane; Polarity; Protein; Rotational dynamics; Spin-label