title: In vivo quantitation of injected circulating tumor cells from great saphenous vein based on video-rate confocal microscopy.
abstract: The number of circulating tumor cell (CTC) in the peripheral blood of cancer patients can be a valuable biomarker for cancer diagnosis and treatment monitoring. In this study, we implemented a custom-design video-rate confocal microscopy system in capable of direct visualization of fast flowing CTC at great saphenous vein (GSV) of a live animal model in vivo. Continuous acquisition of video-rate images at GSV revealed the highly dynamic time-dependent changes in the number of intravenously injected circulating tumor cells. By extracting a calibration factor through the hemocytometric analysis of intravenously injected long-circulating red blood cells, we established a novel quantitation method for CTC in whole body blood in vivo.
authors.affiliation: Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daehak-ro 291, Yuseong-gu, Daejeon, 305-701, South Korea.
authors.forename: Howon
authors.initials: H
authors.lastname: Seo
authors.affiliation: Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daehak-ro 291, Yuseong-gu, Daejeon, 305-701, South Korea.
authors.forename: Yoonha
authors.initials: Y
authors.lastname: Hwang
authors.affiliation: Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daehak-ro 291, Yuseong-gu, Daejeon, 305-701, South Korea.
authors.forename: Kibaek
authors.initials: K
authors.lastname: Choe
authors.affiliation: Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daehak-ro 291, Yuseong-gu, Daejeon, 305-701, South Korea.
authors.forename: Pilhan
authors.initials: P
authors.lastname: Kim
keywords: (000.1430) Biology and medicine; (170.1470) Blood or tissue constituent monitoring; (170.1790) Confocal microscopy; (170.2520) Fluorescence microscopy