title: Dietary compounds galangin and myricetin suppress ovarian cancer cell angiogenesis.
abstract: Galangin and myricetin are flavonoids isolated from vegetables and fruits which exhibit anti-proliferative activity in human cancer cells. In this study, their anti-angiogenic effects were investigated within vitro(HUVEC) andin vivo(CAM) models, which showed that galangin and myricetin inhibited angiogenesis induced by OVCAR-3 cells. The molecular mechanisms through which galangin and myricetin suppress angiogenesis were also studied. It was observed that galangin and myricetin inhibited secretion of the key angiogenesis mediator vascular endothelial growth factor (VEGF) and decreased levels of p-Akt, p-70S6K and hypoxia-inducible factor-1α (HIF-1α) proteins in A2780/CP70 and OVCAR-3 cells. Transient transfection experiments showed that galangin and myricetin inhibited secretion of VEGF by the Akt/p70S6K/ HIF-1α pathway. Moreover, a novel pathway, p21/HIF-1α/VEGF, was found to be involved in the inhibitory effect of myricetin on angiogenesis in OVCAR-3 cells. These data suggest that galangin and myricetin might serve as potential anti-angiogenic agents in the prevention of ovarian cancers dependent on new blood vessel networks.
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang University, China ; College of Science, Technology & Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA.
authors.forename: Haizhi
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Pharmaceutical Science, West Virginia University, Morgantown, WV 26506, USA.
authors.forename: Allen Y
authors.initials: AY
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Science, West Virginia University, Morgantown, WV 26506, USA.
authors.forename: Yon
authors.initials: Y
authors.lastname: Rojanasakul
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang University, China.
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA.
authors.forename: Gary O
authors.initials: GO
authors.lastname: Rankin
authors.affiliation: College of Science, Technology & Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA.
authors.forename: Yi Charlie
authors.initials: YC
authors.lastname: Chen
keywords: Angiogenesis; Galangin; Myricetin; Ovarian cancer; p21