title: Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses.
abstract: Since the recent discovery of Samba virus, the first representative of the family Mimiviridae from Brazil, prospecting for mimiviruses has been conducted in different environmental conditions in Brazil. Recently, we isolated using Acanthamoeba sp. three new mimiviruses, all of lineage A of amoebal mimiviruses: Kroon virus from urban lake water; Amazonia virus from the Brazilian Amazon river; and Oyster virus from farmed oysters. The aims of this work were to sequence and analyze the genome of these new Brazilian mimiviruses (mimi-BR) and update the analysis of the Samba virus genome. The genomes of Samba virus, Amazonia virus and Oyster virus were 97%-99% similar, whereas Kroon virus had a low similarity (90%-91%) with other mimi-BR. A total of 3877 proteins encoded by mimi-BR were grouped into 974 orthologous clusters. In addition, we identified three new ORFans in the Kroon virus genome. Additional work is needed to expand our knowledge of the diversity of mimiviruses from Brazil, including if and why among amoebal mimiviruses those of lineage A predominate in the Brazilian environment.
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. felipelopesassis@gmail.com.
authors.forename: Felipe L
authors.initials: FL
authors.lastname: Assis
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. leenaasd@gmail.com.|Department of Biochemistry, Faculty of Science, King Abdulaziz University, 21589 Jeddah, Saudi Arabia. leenaasd@gmail.com.
authors.forename: Leena
authors.initials: L
authors.lastname: Bajrai
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. jonatas.abrahao@gmail.com.
authors.forename: Jonatas S
authors.initials: JS
authors.lastname: Abrahao
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. ernagkroon@gmail.com.
authors.forename: Erna G
authors.initials: EG
authors.lastname: Kroon
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. fabiopiod154@gmail.com.
authors.forename: Fabio P
authors.initials: FP
authors.lastname: Dornas
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. ketyllen@yahoo.com.br.
authors.forename: Kétyllen R
authors.initials: KR
authors.lastname: Andrade
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil. pvboratto@gmail.com.
authors.forename: Paulo V M
authors.initials: PV
authors.lastname: Boratto
authors.affiliation: Centro de Ciências Biológicas, Departamento de Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil. maryrp@gmail.com.
authors.forename: Mariana R
authors.initials: MR
authors.lastname: Pilotto
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. catherine.robert@univ-amu.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robert
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. benamarsamia@yahoo.fr.
authors.forename: Samia
authors.initials: S
authors.lastname: Benamar
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. bernard.la-scola@univ-amu.fr.|Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Centre Hospitalo-Universitaire Timone, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, 13385 Marseille, France. bernard.la-scola@univ-amu.fr.
authors.forename: Bernard La
authors.initials: BL
authors.lastname: Scola
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France. philippe.colson@univ-amu.fr.|Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique-Hôpitaux de Marseille, Centre Hospitalo-Universitaire Timone, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, 13385 Marseille, France. philippe.colson@univ-amu.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Colson
mesh_terms: D001483:Base Sequence; D001938:Brazil; D005618:Fresh Water / Q000821:virology*; D016679:Genome, Viral; D056686:Mimiviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment
keywords: Amazonia virus; Kroon virus; Megavirales; Mimiviridae; Samba virus; genomics; mimivirus; oyster virus; pan-genome