title: Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma.
abstract: OBJECTIVE
  A disintegrin and metalloprotease (ADAM) 17 has been reported to be implicated in cancer cells invasion. Nevertheless, its potential role in lung adenocarcinoma has not been addressed clearly.
  
  
  METHODS
  RT-PCR and Western blot were used to detect the expression of miR-326 and ADAM17 in lung adenocarcinoma samples (n=73). miR-326 mimics and inhibitor were tansfected in human A549 and SPCA1 cell lines. The transwell assay was used to detect the cell invasive ability. The regulation mechanism was evaluated by luciferase reporter assay. The markers of (epithelial-to-mesenchymal transition) EMT were detected by using Western blot assay.
  
  
  RESULTS
  We found increased expression of ADAM17 in lung adenocarcinoma and cell lines. In vitro, up-regulation of ADAM17 promoted cells invasion, while silencing of ADAM17 inhibited cells invasion. Meanwhile, ADAM17 could affect the markers of EMT. Furthermore, we confirmed that ADAM17 is a target of miR-326, which is involved in EMT and cells invasion.
  
  
  CONCLUSIONS
  These findings revealed that ADAM17, a target of miR-326, promoted EMT-induced cells invasion in lung adenocarcinoma.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Jiangnan University, The Forth People's Hospital of Wuxi City, Wuxi, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cai
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jiru
authors.initials: J
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Zhou
authors.forename: Linfang
authors.initials: L
authors.lastname: Jin
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Bai
authors.forename: Yuan
authors.initials: Y
authors.lastname: Weng
mesh_terms: D051722:ADAM Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000072198:ADAM17 Protein; D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000368:Aged; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D015194:Diffusion Chambers, Culture; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C545322:MIRN326 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D008156:Luciferases; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human