title: Contribution of NADPH oxidase to the establishment of hippocampal neuronal polarity in culture.
abstract: Reactive oxygen species (ROS) produced by the NADPH oxidase (NOX) complex play important physiological and pathological roles in neurotransmission and neurodegeneration, respectively. However, the contribution of ROS to the molecular mechanisms involved in neuronal polarity and axon elongation is not well understood. In this work, we found that loss of NOX complex function altered neuronal polarization and decreased axonal length by a mechanism that involves actin cytoskeleton dynamics. These results indicate that physiological levels of ROS produced by the NOX complex modulate hippocampal neuronal polarity and axonal growth in vitro.
authors.affiliation: Department of Biology, Faculty of Sciences, Universidad de Chile, Las Palmeras 3425, Santiago 7800024, Chile.
authors.forename: Carlos
authors.initials: C
authors.lastname: Wilson
authors.affiliation: Department of Biology, Faculty of Sciences, Universidad de Chile, Las Palmeras 3425, Santiago 7800024, Chile.
authors.forename: M Tulio
authors.initials: MT
authors.lastname: Núñez
authors.affiliation: Department of Biology, Faculty of Sciences, Universidad de Chile, Las Palmeras 3425, Santiago 7800024, Chile chrgonza@uchile.cl.
authors.forename: Christian
authors.initials: C
authors.lastname: González-Billault
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics* / Q000378:metabolism; D001369:Axons / Q000378:metabolism*; D016764:Cell Polarity / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D019255:NADPH Oxidases / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D019255:NADPH Oxidases
keywords: Actin cytoskeleton; NOX; Neuronal polarity; ROS