title: Investigating physiological, cellular and molecular effects in juvenile blue crab, Callinectus sapidus, exposed to field-collected sediments contaminated by oil from the Deepwater Horizon Incident.
abstract: Juvenile blue crabs, Callinectus sapidus, were exposed for 31 days to six different sediments collected within the Pass a Loutre State Wildlife Management Area approximately 6 months or 1.5 years post-capping of the Macondo-252 well-head following the Deepwater Horizon (DWH) Incident. Based on forensic analysis to fingerprint for DWH oil, these sediments differed in their levels of DWH oil contamination, and included one reference sediment collected from a location with no detectable DWH oil present. The concentration of 50 individual parent and alkylation group polycyclic aromatic hydrocarbons (PAHs), saturated hydrocarbons (37 total), and total extractable hydrocarbons were determined in each sediment, as were biologically relevant metals, grain size distribution, percent total organic carbon, and percent total solids. Total concentrations of 50 PAHs (TPAH50) of initial treatment sediments ranged from 187 μg kg(-1) (reference site) to 2,086,458 μg kg(-1) (the highest DWH oil contaminated site). Multiple biological endpoints were measured including mortality, growth, and ecdysis. Additionally, early biomarkers of biological stress were examined in the hemolymph and hepatopancreas of crabs, including DNA damage (Comet assay) and expression of genes encoding Cu-metallothionein (CuMT), glutathione-S-transferase (GST), and manganese superoxide dismutase (MnSOD). Over the 31 day exposure, there were no treatment related mortalities in juvenile blue crabs. The overall growth and molting of the crabs were not substantially different between the various sediment exposures over the exposure period. Additionally, none of the early biomarkers of biological stress were correlated with PAH concentrations. Overall, juvenile blue crabs did not appear to be negatively impacted during the 31 day exposure by DWH oil contaminated sediments collected at least 6 months post-capping of the Macondo-252 well-head.
authors.affiliation: University of Maryland-Baltimore, Graduate Program of Life Sciences, Program in Toxicology, 660W. Redwood St., Rm. 134, Howard Hall, Baltimore, MD 21201 USA; University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, P.O. Box 38, 146 Williams Street, Solomons, MD 20688 USA.
authors.forename: Hannah V
authors.initials: HV
authors.lastname: Pie
authors.affiliation: Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, 701 East Pratt Street, Baltimore, MD 21202 USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Schott
authors.affiliation: University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, P.O. Box 38, 146 Williams Street, Solomons, MD 20688 USA. Electronic address: mitchelmore@umces.edu.
authors.forename: Carys L
authors.initials: CL
authors.lastname: Mitchelmore
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003386:Brachyura / Q000502:physiology*; D004784:Environmental Monitoring; D006838:Hydrocarbons / Q000378:metabolism* / Q000633:toxicity; D008668:Metallothionein / Q000378:metabolism; D059392:Petroleum Pollution; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D006838:Hydrocarbons; D014874:Water Pollutants, Chemical; D008668:Metallothionein
keywords: Blue crab; Deepwater horizon; Gene expression; Polycyclic aromatic hydrocarbons; Sediment