title: Transcriptome response to infraorbital nerve transection in the gonadally intact male rat barrel cortex: RNA-seq.
abstract: The effects of infraorbital nerve (ION) transection on gene expression in the adult male rat barrel cortex were investigated using RNA sequencing. After a 24-hour survival duration, 98 genes were differentially regulated by ION transection. Differentially expressed genes suggest changes in neuronal activity, excitability, and morphology. The production of mRNA for neurotrophins, including brain-derived neurotrophin factor (BNDF), was decreased following ION transection. Several potassium channels showed decreased mRNA production, whereas a sodium channel (Na(V)β4) associated with burst firing showed increased mRNA production. The results may have important implications for phantom-limb pain and complex regional pain syndrome. Future experiments should determine the extent to which changes in RNA result in changes in protein expression, in addition to utilizing laser capture microdissection techniques to differentiate between neuronal and glial cells.
authors.affiliation: Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, 47045.
authors.forename: John J
authors.initials: JJ
authors.lastname: Orczyk
authors.affiliation: Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, 47045.
authors.forename: Rishabh
authors.initials: R
authors.lastname: Sethia
authors.affiliation: Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, 47045.
authors.forename: Dominique
authors.initials: D
authors.lastname: Doster
authors.affiliation: Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, 47045.|Program in Neuroscience, Indiana University, Bloomington, Indiana, 47045.
authors.forename: Preston E
authors.initials: PE
authors.lastname: Garraghty
mesh_terms: D000818:Animals; D015870:Gene Expression; D008297:Male; D008442:Maxillary Nerve / Q000293:injuries*; D009473:Neuronal Plasticity / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012683:Sensory Deprivation / Q000502:physiology*; D013003:Somatosensory Cortex / Q000378:metabolism*; D059467:Transcriptome; D014738:Vibrissae / Q000294:innervation / Q000502:physiology
chemical_list: D012333:RNA, Messenger
keywords: CLC Main Workbench (RRID:OMICS_01813); Ingenuity Pathway Analysis (RRID:nif-0000-33144); Sprague-Dawley rats (RRID:RGD_70508); cerebral cortex; injury; plasticity