title: cir-ITCH plays an inhibitory role in colorectal cancer by regulating the Wnt/β-catenin pathway.
abstract: Noncoding RNAs (ncRNAs) are the dominant product of eukaryotic transcription. These products range from short microRNAs (miRNAs) to long intergenic noncoding RNAs (lincRNAs). Circular RNAs composed of exonic sequences represent an understudied form of ncRNA that was discovered more than 20 years ago. Using a TaqMan-based reverse transcriptase polymerase chain reaction assay, we analyzed the relationship between cir-ITCH expression and colorectal cancer (CRC) in a total of 45 CRCs and paired adjacent non-tumor tissue samples. We found that cir-ITCH expression was typically down-regulated in CRC compared to the peritumoral tissue. This result, as well as several follow-up experiments, showed that cir-ITCH could increase the level of ITCH, which is involved in the inhibition of the Wnt/β-catenin pathway. Therefore, our results showed that cir-ITCH plays a role in CRC by regulating the Wnt/β-catenin pathway.
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
authors.forename: Guanli
authors.initials: G
authors.lastname: Huang
authors.affiliation: Departments of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
authors.forename: Yixiong
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of endoscopic surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of endoscopic surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
authors.forename: Xiangjian
authors.initials: X
authors.lastname: Chen
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D001665:Binding Sites; D002470:Cell Survival; D015179:Colorectal Neoplasms / Q000378:metabolism*; D003609:Dactinomycin / Q000737:chemistry; D005095:Exoribonucleases / Q000737:chemistry; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D010957:Plasmids / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D000079962:RNA, Circular; D022661:RNA, Untranslated / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D000079962:RNA, Circular; D022661:RNA, Untranslated; D051153:Wnt Proteins; D051176:beta Catenin; C000607101:cir-ITCH noncoding RNA, human; D003609:Dactinomycin; D012313:RNA; D005095:Exoribonucleases; C080868:ribonuclease R