title: PPR protein PDM1/SEL1 is involved in RNA editing and splicing of plastid genes in Arabidopsis thaliana.
abstract: After transcription, most chloroplast precursor RNAs undergo further post-transcriptional processing including cleavage, editing, and splicing. Previous investigation has shown that the cleavage of the rpoA transcript and most editing sites, including accD-1, are defective in the knockout mutant of PDM1/SEL1, a PLS-type PPR protein, and that PDM1 is associated with the rpoA transcript. In this work, we found that the splicing of group II introns in trnK and ndhA is also affected in pdm1. Co-immunoprecipitation mass spectrometry experiments were performed to identify proteins that are associated with PDM1. We obtained 126 non-redundant proteins, of which MORF9 was reported to be involved in RNA editing in chloroplast. Yeast two-hybrid assays showed that PDM1 interacts directly with MORF9, MORF2, and MORF8. RNA immunoprecipitation showed that PDM1 associates with the transcripts of trnK and ndhA, as well as accD-1, suggesting that PDM1 is involved in RNA editing and splicing. Therefore, PDM1 is an important protein for post-transcriptional regulation in chloroplast.
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Hong-Dao
authors.initials: HD
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Yong-Lan
authors.initials: YL
authors.lastname: Cui
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Yin
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Qin
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Te
authors.initials: T
authors.lastname: Xu
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: He
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Zi-Ran
authors.initials: ZR
authors.lastname: Li
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China. znyang@shnu.edu.cn.
authors.forename: Zhong-Nan
authors.initials: ZN
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D018087:Plastids / Q000235:genetics; D011499:Protein Processing, Post-Translational; D017393:RNA Editing; D012326:RNA Splicing; D018749:RNA, Plant / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D018749:RNA, Plant; D017382:Reactive Oxygen Species; C000590497:SEL1 protein, Arabidopsis; C538752:pentatricopeptide repeat protein, Arabidopsis
keywords: Chloroplast; PPR protein; RNA editing; ROS; Splicing