title: Lower serum soluble-EGFR is a potential biomarker for metastasis of HCC demonstrated by N-glycoproteomic analysis.
abstract: Hepatocellular carcinoma (HCC) is one of the most deadly cancers in the world due to its high metastatic potential. By using the isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative N-glycoproteomic analysis, 26 differentially expressed serum glycoproteins derived from defined stages in orthotopic xenograft tumor model were identified. Among them, expression level of soluble EGFR (sEGFR) was verified in HCC cell lines. We found that non-metastasis HCC cell lines express significantly more sEGFR than HCC cell lines with metastasis potential both in cell lysates and culture media. Serum samples from 28 non-metastatic HCC patients and 28 metastatic HCC patients were assayed. Compared with the non-metastatic HCC group, serum level of sEGFR in metastatic HCC group was statistically lower (p<0.01). All these results provide evidence that sEGFR is a potential candidate for metastasis-associated biomarkers of HCC. The related molecular mechanism deserves to be further explored.
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital; and Cancer Research Center, Institute of Biomedical Sciences; Fudan University, Shanghai, 200032, China.
authors.forename: Cun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital; and Cancer Research Center, Institute of Biomedical Sciences; Fudan University, Shanghai, 200032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital; and Cancer Research Center, Institute of Biomedical Sciences; Fudan University, Shanghai, 200032, China.
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Chunmin
authors.initials: C
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D002457:Cell Extracts; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D066246:ErbB Receptors / Q000097:blood* / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012995:Solubility; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D002457:Cell Extracts; D006023:Glycoproteins; C512478:EGFR protein, human; D066246:ErbB Receptors