title: Allelic variation at the rpv1 locus controls partial resistance to Plum pox virus infection in Arabidopsis thaliana.
abstract: BACKGROUND
  Sharka is caused by Plum pox virus (PPV) in stone fruit trees. In orchards, the virus is transmitted by aphids and by grafting. In Arabidopsis, PPV is transferred by mechanical inoculation, by biolistics and by agroinoculation with infectious cDNA clones. Partial resistance to PPV has been observed in the Cvi-1 and Col-0 Arabidopsis accessions and is characterized by a tendency to escape systemic infection. Indeed, only one third of the plants are infected following inoculation, in comparison with the susceptible Ler accession.
  
  
  RESULTS
  Genetic analysis showed this partial resistance to be monogenic or digenic depending on the allelic configuration and recessive. It is detected when inoculating mechanically but is overcome when using biolistic or agroinoculation. A genome-wide association analysis was performed using multiparental lines and 147 Arabidopsis accessions. It identified a major genomic region, rpv1. Fine mapping led to the positioning of rpv1 to a 200 kb interval on the long arm of chromosome 1. A candidate gene approach identified the chloroplast phosphoglycerate kinase (cPGK2) as a potential gene underlying the resistance. A virus-induced gene silencing strategy was used to knock-down cPGK2 expression, resulting in drastically reduced PPV accumulation.
  
  
  CONCLUSIONS
  These results indicate that rpv1 resistance to PPV carried by the Cvi-1 and Col-0 accessions is linked to allelic variations at the Arabidopsis cPGK2 locus, leading to incomplete, compatible interaction with the virus.
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. poque.sylvain@gmail.com.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. poque.sylvain@gmail.com.|Current address: Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan. poque.sylvain@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Poque
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. gaelle.pagny@yahoo.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. gaelle.pagny@yahoo.fr.
authors.forename: G
authors.initials: G
authors.lastname: Pagny
authors.affiliation: INRA-UR1052, Genetics and Breeding of Fruits and Vegetables, Dom. St Maurice, CS 60094, F-84143, Montfavet cedex, France. laurence.ouibrahim@hotmail.com.
authors.forename: L
authors.initials: L
authors.lastname: Ouibrahim
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. achague@bordeaux.inra.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. achague@bordeaux.inra.fr.
authors.forename: A
authors.initials: A
authors.lastname: Chague
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. eyquard@bordeaux.inra.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. eyquard@bordeaux.inra.fr.
authors.forename: J-P
authors.initials: JP
authors.lastname: Eyquard
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. mbousque@bordeaux.inra.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. mbousque@bordeaux.inra.fr.
authors.forename: M
authors.initials: M
authors.lastname: Caballero
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. candress@bordeaux.inra.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. candress@bordeaux.inra.fr.
authors.forename: T
authors.initials: T
authors.lastname: Candresse
authors.affiliation: INRA-UR1052, Genetics and Breeding of Fruits and Vegetables, Dom. St Maurice, CS 60094, F-84143, Montfavet cedex, France. Carole.Caranta@avignon.inra.fr.
authors.forename: C
authors.initials: C
authors.lastname: Caranta
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. smariett@bordeaux.inra.fr.|Current address: INRA, UMR 1202 Biogeco, F- 33610, Cestas, France. smariett@bordeaux.inra.fr.|Current address: Univ. Bordeaux, UMR1202 Biogeco, F-33400, Talence, France. smariett@bordeaux.inra.fr.
authors.forename: S
authors.initials: S
authors.lastname: Mariette
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. decroocq@bordeaux.inra.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33140, Villenave d'Ornon, cedex, France. decroocq@bordeaux.inra.fr.
authors.forename: V
authors.initials: V
authors.lastname: Decroocq
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000821:virology*; D019470:Biolistics; D002736:Chloroplasts / Q000201:enzymology; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D060146:Ecotype; D020868:Gene Silencing; D017343:Genes, Plant; D056426:Genetic Loci; D055106:Genome-Wide Association Study; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D018515:Plant Leaves / Q000201:enzymology / Q000821:virology; D030821:Plants, Genetically Modified; D017801:Plum Pox Virus / Q000502:physiology*; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000821:virology
chemical_list: D049452:Green Fluorescent Proteins; D010735:Phosphoglycerate Kinase