title: Gadd45b and N-methyl-D-aspartate induced DNA demethylation in postmitotic neurons.
abstract: OBJECTIVE
  In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf.
  
  
  METHODS
  Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were treated with N-methyl-d-aspartate (NMDA). Expression changes of genes reportedly involved in DNA demethylation, Bdnf mRNA and protein and 5MC and 5HMC at Bdnf promoters were measured.
  
  
  RESULTS
  Gadd45b siRNA transfection in neurons abolishes the NMDA-induced increase in Bdnf IXa mRNA and reductions in 5MC and 5HMC at the Bdnf IXa promoter.
  
  
  CONCLUSIONS
  These results contribute to our understanding of DNA demethylation mechanisms in neurons, and its role in regulating NMDA responsive genes implicated in mental illnesses.
authors.affiliation: Jesse Brown Veterans Affairs Medical Center, 820 South Damen Avenue (M/C 151), Chicago, IL 60612, USA.|Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Gavin
authors.affiliation: Jesse Brown Veterans Affairs Medical Center, 820 South Damen Avenue (M/C 151), Chicago, IL 60612, USA.|Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA.
authors.forename: Handojo
authors.initials: H
authors.lastname: Kusumo
authors.affiliation: Jesse Brown Veterans Affairs Medical Center, 820 South Damen Avenue (M/C 151), Chicago, IL 60612, USA.|Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA.
authors.forename: Rajiv P
authors.initials: RP
authors.lastname: Sharma
authors.affiliation: Jesse Brown Veterans Affairs Medical Center, 820 South Damen Avenue (M/C 151), Chicago, IL 60612, USA.|Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA.|Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR and VA Portland Health Care System, Portland, OR.
authors.forename: Marina
authors.initials: M
authors.lastname: Guizzetti
authors.affiliation: Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Guidotti
authors.affiliation: Jesse Brown Veterans Affairs Medical Center, 820 South Damen Avenue (M/C 151), Chicago, IL 60612, USA.|Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA.
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Pandey
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D019175:DNA Methylation; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism*; D000097642:GADD45 Proteins
chemical_list: D044503:5-Methylcytosine; D019208:Brain-Derived Neurotrophic Factor; D018797:Cell Cycle Proteins; D018690:Excitatory Amino Acid Agonists; D016202:N-Methylaspartate; D009687:Nuclear Proteins; C499072:Gadd45a protein, mouse; D000097642:GADD45 Proteins
keywords: CpG; alcoholism; epigenetic; histone; schizophrenia