title: Differential expression of Marek's disease virus (MDV) late proteins during in vitro and in situ replication: role for pUL47 in regulation of the MDV UL46-UL49 gene locus.
abstract: Marek's disease virus (MDV) is a lymphotropic alphaherpesvirus that replicates in a highly cell-associated manner in vitro. Production of infectious cell-free virus only occurs in feather follicle epithelial (FFE) cells of infected chicken skins. Previously, we described differential expression for a core alphaherpesvirus protein, pUL47 that was found to be abundantly expressed in FFE cells of infected chickens, while barely detectable during in vitro propagation. Here, we further examined the dynamics of expression of four tegument proteins within the UL46-49 locus during in vitro and in situ replication. All four proteins examined were expressed abundantly in situ, whereas both pUL47 and pUL48 expression were barely detectable in vitro. Replacement of the putative UL47 and UL48 promoters with the minimal cytomegalovirus promoter enhanced mRNA and protein expression in vitro. Interestingly, enhanced expression of pUL47 resulted in increased UL46, UL48, and UL49 transcripts that resulted in increased pUL46 and pUL48 expression.
authors.affiliation: Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa, IA, USA. Electronic address: keith-jarosinski@uiowa.edu.
authors.forename: Keith W
authors.initials: KW
authors.lastname: Jarosinski
authors.affiliation: INRA, UMR1282, Infectious Diseases and Public Health, ISP, BIOVA Team, Nouzilly, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Vautherot
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004847:Epithelial Cells / Q000821:virology; D015967:Gene Expression Regulation, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000378:metabolism*
chemical_list: D015678:Viral Structural Proteins
keywords: Chicken; Fluorescent protein; Gene expression; Herpesvirus; Marek’s disease; Recombinant; pUL46; pUL47; pUL48; pUL49