title: A practical and highly sensitive C3N4-TYR fluorescent probe for convenient detection of dopamine.
abstract: The C3N4-tyrosinase (TYR) hybrid is a highly accurate, sensitive and simple fluorescent probe for the detection of dopamine (DOPA). Under optimized conditions, the relative fluorescence intensity of C3N4-TYR is proportional to the DOPA concentration in the range from 1 × 10(-3) to 3 × 10(-8) mol L(-1) with a correlation coefficient of 0.995. In the present system, the detection limit achieved is as low as 3 × 10(-8) mol L(-1). Notably, these quantitative detection results for clinical samples are comparable to those of high performance liquid chromatography. Moreover, the enzyme-encapsulated C3N4 sensing arrays on both glass slide and test paper were evaluated, which revealed sensitive detection and excellent stability. The results reported here provide a new approach for the design of a multifunctional nanosensor for the detection of bio-molecules.
authors.affiliation: Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, PR China. zhkang@suda.edu.cn yangl@suda.edu.cn hhuang0618@suda.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Manman
authors.initials: M
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Yalin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Kang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D014442:Monophenol Monooxygenase; D004298:Dopamine