title: Osteogenic differentiation of MC3T3-E1 cells on poly(L-lactide)/Fe3O4 nanofibers with static magnetic field exposure.
abstract: Proliferation and differentiation of bone-related cells are modulated by many factors such as scaffold design, growth factor, dynamic culture system, and physical simulation. Nanofibrous structure and moderate-intensity (1 mT-1 T) static magnetic field (SMF) have been identified as capable of stimulating proliferation and differentiation of osteoblasts. Herein, magnetic nanofibers were prepared by electrospinning mixture solutions of poly(L-lactide) (PLLA) and ferromagnetic Fe3O4 nanoparticles (NPs). The PLLA/Fe3O4 composite nanofibers demonstrated homogeneous dispersion of Fe3O4 NPs, and their magnetism depended on the contents of Fe3O4 NPs. SMF of 100 mT was applied in the culture of MC3T3-E1 osteoblasts on pure PLLA and PLLA/Fe3O4 composite nanofibers for the purpose of studying the effect of SMF on osteogenic differentiation of osteoblastic cells on magnetic nanofibrous scaffolds. On non-magnetic PLLA nanofibers, the application of external SMF could enhance the proliferation and osteogenic differentiation of MC3T3-E1 cells. In comparison with pure PLLA nanofibers, the incorporation of Fe3O4 NPs could also promote the proliferation and osteogenic differentiation of MC3T3-E1 cells in the absence or presence of external SMF. The marriage of magnetic nanofibers and external SMF was found most effective in accelerating every aspect of biological behaviors of MC3T3-E1 osteoblasts. The findings demonstrated that the magnetic feature of substrate and microenvironment were applicable ways in regulating osteogenesis in bone tissue engineering.
authors.affiliation: State Key Laboratory of Organic-inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China; Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Dingying
authors.initials: D
authors.lastname: Shan
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Jia
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Shun
authors.initials: S
authors.lastname: Duan
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, PR China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Organic-inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China; Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: yangxp@mail.buct.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005290:Ferric Compounds / Q000737:chemistry*; D060526:Magnetic Fields; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D011091:Polyesters; C000499:ferric oxide; C033616:poly(lactide)
keywords: Magnetic nanofibers; Moderate-intensity; Osteogenic differentiation; Static magnetic field