title: Interaction of intraocular lenses with fibronectin and human lens epithelial cells: Effect of chemical composition and aging.
abstract: The aim of this study is to investigate in vitro interactions between hydrophobic acrylate intraocular lenses (IOLs) and their biological environment. The influence of lens chemical composition and aging on fibronectin (FN) adsorption and on IOLs cytotoxicity on human lens epithelial cells was examined. Cytotoxicity of acrylate monomers used in IOLs manufacture was also investigated. Four different IOLs were included in the study: Acrysof(®), Tecnis(®), EnVista(®), and iSert(®). Implants were artificially aged in a xenon arc chamber to simulate 2 years of light exposure. Fibronectin adsorption on IOL surface was quantified using ELISA and correlated to surface roughness determined with AFM. Direct contact cytotoxicity was determined with the MTT assay and cell morphology was observed with light microscopy. Results showed that fibronectin adsorption did not differ significantly among IOLs, whatever their chemical composition. Moreover, aging conditions did not impact fibronectin adsorption. All IOLs were biocompatible even after applying 2-year aging conditions, with cell viability higher than 70%. Five acrylate monomers appeared to be toxic in the range of concentrations tested, but no monomer release from the IOLs could be detected during accelerated 2-year incubation with saline solution. This study did not reveal an influence of chemical composition and aging on protein adsorption and on biocompatibility.
authors.affiliation: Université Paris-Sud, EA401 Matériaux et Santé, IFR 141, Faculté De Pharmacie, Châtenay Malabry, F-92296, France.|Gustave Roussy Cancer Institute, Pharmacy Department, Villejuif, F-94800, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Tortolano
authors.affiliation: Université Paris-Sud, EA401 Matériaux et Santé, IFR 141, Faculté De Pharmacie, Châtenay Malabry, F-92296, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Serrano
authors.affiliation: Université Paris-Sud, EA401 Matériaux et Santé, IFR 141, Faculté De Pharmacie, Châtenay Malabry, F-92296, France.
authors.forename: Emile
authors.identifier: http://orcid.org/0000-0003-2855-4091
authors.initials: E
authors.lastname: Jubeli
authors.affiliation: Université Paris-Sud, EA401 Matériaux et Santé, IFR 141, Faculté De Pharmacie, Châtenay Malabry, F-92296, France.
authors.forename: Johanna
authors.initials: J
authors.lastname: Saunier
authors.affiliation: Université Paris-Sud, EA401 Matériaux et Santé, IFR 141, Faculté De Pharmacie, Châtenay Malabry, F-92296, France.
authors.forename: Najet
authors.initials: N
authors.lastname: Yagoubi
mesh_terms: D000179:Acrylates / Q000378:metabolism* / Q000633:toxicity; D001672:Biocompatible Materials / Q000378:metabolism* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism; D007910:Lenses, Intraocular / Q000009:adverse effects; D012826:Silicone Elastomers
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D005353:Fibronectins; D012826:Silicone Elastomers; C036658:acrylic acid
keywords: biocompatibility; hydrophobic acrylic intraocular lenses; lens epithelial cells; polymer aging