title: Observed impacts of insulin therapy on callus cell transforming growth factor-beta 1 expression in diabetic rats.
abstract: The expression of transforming growth factor-beta 1 (TGF-β1) inside the callus cells of diabetic rats and the impact of insulin therapy on its expression and biomechanics was investigated. The rats were randomly divided as follows: an insulin therapy group (IT), a diabetic model group (DM), and a non-diabetic control group (NC). Bone specimens from each group were extracted at different times for immunohistochemical observation of the expression of TGF-β1. Concurrently, the destruction torque and torsional stiffness were detected at different times. One to four weeks after fracture, TGF-β1 was widely expressed in fractured callus cells and periosteal proliferating cells, while the expression inside diabetic cells was significantly reduced. The expression of TGF-β1 decreased over the first 68 weeks, and the mature bone cells never expressed TGF-β1. The destruction torque (Nm) detected in the 6th week revealed that there was a statistically significant difference between the DM, NC, and IT groups (P < 0.01). In conclusion, TGF-β1 expression was significantly reduced inside the callus cells of diabetic rats. Insulin therapy increased TGF-β1 expression inside the callus cells of diabetic rats and improved the biomechanical characteristics of the callus.
authors.affiliation: Central Sterile Supply Department, Liao Cheng People's Hospital, Liao Cheng, Shandong, China.
authors.forename: L X
authors.initials: LX
authors.lastname: Wang
authors.affiliation: Intensive Care Unit, Liao Cheng People's Hospital, Liao Cheng, Shandong, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Liao Cheng People's Hospital, Shandong, China ShunleiDu@163.com.
authors.forename: S L
authors.initials: SL
authors.lastname: Du
mesh_terms: D000818:Animals; D002146:Bony Callus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D017102:Fracture Healing / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006244:Hardness; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D010521:Periosteum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013978:Tibial Fractures / Q000150:complications / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D019415:Torque; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D053773:Transforming Growth Factor beta1