title: Role of RHOT1 on migration and proliferation of pancreatic cancer.
abstract: Pancreatic cancer (PC) is one of the most malignant tumors. Rho GTPases can affect several types of human cancers, including PC. In this study, we investigated the role of Ras homolog family member T1 (RHOT1), a new member of Rho GTPases in PC. IHC results showed that RHOT1 was expressed significantly higher in PC tissues than paracancerous tissues (P<0.01) and SMAD family member 4 (SMAD4) was expressed lower in PC tissues (P<0.01). RHOT1 was widely expressed in PC cell lines analyzed by reverse transcription PCR (RT-PCR), real-time quantitative PCR (RT-qPCR) and western blotting (WB). SiRNA-RHOT1 significantly suppressed the proliferation and migration of SW1990 cells. Moreover, SMAD4 was identified as an effector of RHOT1. Our findings suggest that RHOT1 can regulate cell migration and proliferation by suppressing the expression of SMAD4 in PC, which may provide a novel sight to explore the mechanism and therapeutic strategy for PC.
authors.affiliation: Department of Geriatrics, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Youzhen
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Geriatrics, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Geng
authors.affiliation: Department of Gastroenterology, Institute of Digestive Diseases, Tongji Hospital Affiliated to Tongji University Shanghai 200065, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Geriatrics, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
keywords: RHOT1; SMAD4; migration; pancreatic cancer; proliferation