title: Development of a full automation solid phase microextraction method for investigating the partition coefficient of organic pollutant in complex sample.
abstract: A fully automated solid phase microextraction (SPME) depletion method was developed to study the partition coefficient of organic compound between complex matrix and water sample. The SPME depletion process was conducted by pre-loading the fiber with a specific amount of organic compounds from a proposed standard gas generation vial, and then desorbing the fiber into the targeted samples. Based on the proposed method, the partition coefficients (Kmatrix) of 4 polyaromatic hydrocarbons (PAHs) between humic acid (HA)/hydroxypropyl-β-cyclodextrin (β-HPCD) and aqueous sample were determined. The results showed that the logKmatrix of 4 PAHs with HA and β-HPCD ranged from 3.19 to 4.08, and 2.45 to 3.15, respectively. In addition, the logKmatrix values decreased about 0.12-0.27 log units for different PAHs for every 10°C increase in temperature. The effect of temperature on the partition coefficient followed van't Hoff plot, and the partition coefficient at any temperature can be predicted based on the plot. Furthermore, the proposed method was applied for the real biological fluid analysis. The partition coefficients of 6 PAHs between the complex matrices in the fetal bovine serum and water were determined, and compared to ones obtained from SPME extraction method. The result demonstrated that the proposed method can be applied to determine the sorption coefficients of hydrophobic compounds between complex matrix and water in a variety of samples.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Jiang
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Wen
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Tiangang
authors.initials: T
authors.lastname: Luan
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: cesoygf@mail.sysu.edu.cn.
authors.forename: Gangfeng
authors.initials: G
authors.lastname: Ouyang
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D004784:Environmental Monitoring / Q000379:methods*; D006812:Humic Substances / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D009930:Organic Chemicals / Q000032:analysis; D052617:Solid Phase Microextraction; D013696:Temperature; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D047392:beta-Cyclodextrins / Q000032:analysis
chemical_list: D006812:Humic Substances; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical; D047392:beta-Cyclodextrins; D014867:Water; D000073738:2-Hydroxypropyl-beta-cyclodextrin
keywords: Automation; Complex matrix; Depletion method; Partition coefficient; Solid phase microextraction