title: Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro.
abstract: Exposure to diesel exhaust particles (DEP) has been associated with adverse cardiopulmonary health effects, which may be related to dysregulation of lipid metabolism and formation of macrophage foam cells. In this study, THP-1 derived macrophages were exposed to an automobile generated DEP (A-DEP) for 24h to study lipid droplet formation and possible mechanisms. The results show that A-DEP did not induce cytotoxicity. The production of reactive oxygen species was only significantly increased after exposure for 3h, but not 24h. Intracellular level of reduced glutathione was increased after 24h exposure. These results combined indicate an adaptive response to oxidative stress. Exposure to A-DEP was associated with significantly increased formation of lipid droplets, as well as changes in lysosomal function, assessed as reduced LysoTracker staining. In conclusion, these results indicated that exposure to A-DEP may induce formation of lipid droplets in macrophages in vitro possibly via lysosomal dysfunction.
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark. Electronic address: yica@sund.ku.dk.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
authors.forename: Kim
authors.initials: K
authors.lastname: Jantzen
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
authors.forename: Ana Cecilia Damiao
authors.initials: AC
authors.lastname: Gouveia
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
authors.forename: Astrid
authors.initials: A
authors.lastname: Skovmand
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Roursgaard
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
authors.forename: Steffen
authors.initials: S
authors.lastname: Loft
authors.affiliation: Department of Environmental Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Møller
mesh_terms: D001336:Automobiles; D002460:Cell Line; D006801:Humans; D066298:In Vitro Techniques; D008247:Lysosomes / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D018384:Oxidative Stress; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D001335:Vehicle Emissions
keywords: Automobile diesel exhaust particles; Lipid droplet; Lysosomal dysfunction; Oxidative stress