title: Effects of conjugated linoleic acid on the expression levels of miR-27 and miR-143 in pig adipose tissue.
abstract: In this study, we evaluated the effect and possible mech-anism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were col-lected, and real-time polymerase chain reaction (PCR) was used to de-termine the expression of adipocyte differentiation marker genes and associated microRNAs (miRNAs). Our results indicated that dietary CLA significantly decreased body fat deposition in the pig dorsum. The expression of adipocyte differentiation marker genes, including peroxi-some proliferator-activated receptor (PPAR)-γ and CCAAT/enhancer-binding protein α (C/EBPα) were not affected, whereas the expression of fatty acid binding protein 4 (FABP4) was significantly enhanced (P < 0.05). The expression of miR-27 and miR-143 in adipose tissue was significantly decreased. Data analysis indicated a significant negative correlation between miR-27 and FABP4 expression in the dorsal sub-cutaneous adipose tissue. In addition, the expression of miR-143 and miR-27 exhibited a significant negative relationship with FABP4 and PPARγ in the abdominal subcutaneous adipose tissue. Thus, miRNA levels in adipose tissues could be modulated by CLA, thereby affecting adipose metabolism.
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing, China.
authors.forename: R L
authors.initials: RL
authors.lastname: Qi
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Huang
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing, China yfeiyun@yeah.net.
authors.forename: F Y
authors.initials: FY
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D050218:Body Fat Distribution; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements; D050556:Fatty Acid-Binding Proteins / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D050356:Lipid Metabolism / Q000187:drug effects; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D050556:Fatty Acid-Binding Proteins; D044243:Linoleic Acids, Conjugated; D035683:MicroRNAs; D047495:PPAR gamma