title: Microchip with an open tubular immobilized ph gradient for UV whole column imaging detection.
abstract: This study reports a new method for establishing an open tubular IPG in a microchip coupled with a whole column image detection (WCID) system for protein separation applications. This method allows a wider range of immobilized pH (2.6-9.5) to be established in a PDMS/quartz channel by controlling the diffusion of acidic and basic polymer solutions into the channel through well-designed channel dimensions. The developed pH gradient was experimentally validated by performing the separation of a mixture of standard pI markers. It was further validated by the separation of the hemoglobin control AFSC sample. This method is advantageous over existing IPG methods because it has a wider range of pH and maintains the open tubular feature that matches the UV WCID to improve the sensitivity.
authors.affiliation: Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, Canada.
authors.forename: Hulie
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, Canada.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Glawdel
authors.affiliation: Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, Canada.
authors.forename: Carolyn L
authors.initials: CL
authors.lastname: Ren
mesh_terms: D004867:Equipment Design; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008956:Models, Chemical; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation* / Q000379:methods
chemical_list: D006454:Hemoglobins
keywords: IPG; Microfluidic chip; Protein separation; Whole column image detection