title: Decreased TIM-3 mRNA expression in peripheral blood mononuclear cells from nephropathy patients.
abstract: Increasing evidence shows that TIM-1 and TIM-3 in-fluence chronic autoimmune diseases, and their expression levels in immune cells from nephritic patients are still unknown. Real-time transcription-polymerase chain reaction analysis was used to deter-mine expression levels of TIM-1 and TIM-3 mRNA in peripheral blood mononuclear cells (PBMCs) from 36 patients with minimal change glo-merulopathy (MCG), 65 patients with lupus nephritis (LN), 78 patients with IgA nephropathy (IgAN), 55 patients with membranous nephropa-thy (MN), 22 patients with crescentic glomerulonephritis (CGN), 26 patients with anaphylactoid purpura nephritis (APN), and 63 healthy controls. TIM-3 mRNA expression significantly decreased in PBMCs from nephritic patients (LN, P < 0.0001; MCG, P < 0.0001; MN, P = 0.0031; CGN, P = 0.0464; IgAN, P = 0.0002; APN, P = 0.0392) com-pared with healthy controls. In contrast, there was no significant differ-ence in TIM-1 mRNA expression between the patients and the healthy controls. Our results suggest that insufficient expression of TIM-3 mRNA may be involved in the pathogenesis of nephropathy.
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China xzcai@mail.cmu.edu.cn.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Cai
authors.affiliation: Department of Nephrology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: N
authors.initials: N
authors.lastname: Liu
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Du
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: S
authors.initials: S
authors.lastname: Yu
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005922:Glomerulonephritis, IGA / Q000097:blood* / Q000235:genetics / Q000473:pathology; D000072596:Hepatitis A Virus Cellular Receptor 1; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D007963:Leukocytes, Mononuclear; D008181:Lupus Nephritis / Q000097:blood* / Q000235:genetics / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000097:blood; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000097:blood; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood; D011991:Receptors, Virus / Q000096:biosynthesis* / Q000097:blood
chemical_list: C491143:HAVCR1 protein, human; C448908:HAVCR2 protein, human; D000072596:Hepatitis A Virus Cellular Receptor 1; D000072597:Hepatitis A Virus Cellular Receptor 2; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D011991:Receptors, Virus