title: Colocalization of low-methylesterified pectins and Pb deposits in the apoplast of aspen roots exposed to lead.
abstract: Low-methylesterified homogalacturonans have been suggested to play a role in the binding and immobilization of Pb in CW. Using root apices of hybrid aspen, a plant with a high phytoremediation potential, as a model, we demonstrated that the in situ distribution pattern of low-methylesterified homogalacturonan, pectin epitope (JIM5-P), reflects the pattern of Pb occurrence. The region which indicated high JIM5-P level corresponded with "Pb accumulation zone". Moreover, JIM5-P was especially abundant in cell junctions, CWs lining the intercellular spaces and the corners of intercellular spaces indicating the highest accumulation of Pb. Furthermore, JIM5-P and Pb commonly co-localized. The observations indicate that low-methylesterified homogalacturonan is the CW polymer that determines the capacity of CW for Pb sequestration. Our results suggest a promising directions for CW modification for enhancing the efficiency of plant roots in Pb accumulation, an important aspect in the phytoremediation of soils contaminated with trace metals.
authors.affiliation: Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Rabęda
authors.affiliation: Laboratory of Electron Microscopy, Nencki Institute of Experimental Biology, Pasteur Street 3, 02-093 Warszawa, Poland.
authors.forename: Henryk
authors.initials: H
authors.lastname: Bilski
authors.affiliation: Umea Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90183 Umea, Sweden.
authors.forename: Ewa J
authors.initials: EJ
authors.lastname: Mellerowicz
authors.affiliation: Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Napieralska
authors.affiliation: Laboratory of Electron Microscopy, Nencki Institute of Experimental Biology, Pasteur Street 3, 02-093 Warszawa, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Suski
authors.affiliation: Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Woźny
authors.affiliation: Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland. Electronic address: magdak@amu.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Krzesłowska
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D001673:Biodegradation, Environmental; D015415:Biomarkers / Q000097:blood; D004951:Esterification; D007854:Lead / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D032107:Populus / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D012989:Soil Pollutants; D007854:Lead; D010368:Pectins; C003181:polygalacturonic acid
keywords: Cell wall; Heavy metal; Phytoremediation; Populus; Tolerance