title: Anti-inflammatory tirucallane saponins from Paramignya scandens.
abstract: Five new tirucallane saponins, paramignyosides A-E (1-5), were isolated from the water fraction of the Paramignya scandens stem and leaves. Their structures were elucidated on the basis of spectroscopic evidence including high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and one dimensional (1D)- and 2D-NMR. The effects of isolated compounds on pro-inflammatory cytokines were evaluated by measuring the production of interleukin (IL)-12 p40, IL-6, and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs). Paramignyoside C (3) exhibited selective and potent inhibitory effect (IC50=5.03±0.19 µM) on the production of IL-12 p40 comparable to that of the positive control, SB203580 (IC50=5.00±0.16 µM). Further studies are required to confirm efficacy in vivo and the mechanism of anti-inflammatory effects.
authors.affiliation: Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology (VAST).
authors.forename: Nguyen Huu Toan
authors.initials: NH
authors.lastname: Phan
authors.forename: Nguyen Thi Dieu
authors.initials: NT
authors.lastname: Thuan
authors.forename: Ninh Thi
authors.initials: NT
authors.lastname: Ngoc
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.forename: Sohyun
authors.initials: S
authors.lastname: Kim
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Thanh
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D008070:Lipopolysaccharides / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D008968:Molecular Conformation; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D027821:Rutaceae / Q000737:chemistry* / Q000378:metabolism; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D012503:Saponins; D014315:Triterpenes; C514393:tirucallane