title: Exosomes released by keratinocytes modulate melanocyte pigmentation.
abstract: Cells secrete extracellular vesicles (EVs), exosomes and microvesicles, which transfer proteins, lipids and RNAs to regulate recipient cell functions. Skin pigmentation relies on a tight dialogue between keratinocytes and melanocytes in the epidermis. Here we report that exosomes secreted by keratinocytes enhance melanin synthesis by increasing both the expression and activity of melanosomal proteins. Furthermore, we show that the function of keratinocyte-derived exosomes is phototype-dependent and is modulated by ultraviolet B. In sum, this study uncovers an important physiological function for exosomes in human pigmentation and opens new avenues in our understanding of how pigmentation is regulated by intercellular communication in both healthy and diseased states.
authors.affiliation: 1] Institut Curie, PSL Research University, UMR144, CNRS, F-75248 Paris, France [2] Structure and Membrane Compartments, Centre National de la Recherche Scientifique, UMR144, Paris F-75248, France [3] Cell and Tissue Imaging Facility, Infrastructures en Biologie Santé et Agronomie (IBiSA), Paris F-75248, France.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Lo Cicero
authors.affiliation: 1] Institut Curie, PSL Research University, UMR144, CNRS, F-75248 Paris, France [2] Structure and Membrane Compartments, Centre National de la Recherche Scientifique, UMR144, Paris F-75248, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Delevoye
authors.affiliation: 1] Institut Curie, PSL Research University, UMR144, CNRS, F-75248 Paris, France [2] Structure and Membrane Compartments, Centre National de la Recherche Scientifique, UMR144, Paris F-75248, France.
authors.forename: Floriane
authors.initials: F
authors.lastname: Gilles-Marsens
authors.affiliation: Institut Curie, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris F-75248, France.
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.affiliation: Institut Curie, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris F-75248, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Dingli
authors.affiliation: Laboratoires Clarins-31 chaussée Jules César, Pontoise 95300, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Guéré
authors.affiliation: Laboratoires Clarins-31 chaussée Jules César, Pontoise 95300, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: André
authors.affiliation: Laboratoires Clarins-31 chaussée Jules César, Pontoise 95300, France.
authors.forename: Katell
authors.initials: K
authors.lastname: Vié
authors.affiliation: 1] Institut Curie, PSL Research University, UMR144, CNRS, F-75248 Paris, France [2] Structure and Membrane Compartments, Centre National de la Recherche Scientifique, UMR144, Paris F-75248, France [3] Cell and Tissue Imaging Facility, Infrastructures en Biologie Santé et Agronomie (IBiSA), Paris F-75248, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: van Niel
authors.affiliation: 1] Institut Curie, PSL Research University, UMR144, CNRS, F-75248 Paris, France [2] Structure and Membrane Compartments, Centre National de la Recherche Scientifique, UMR144, Paris F-75248, France [3] Cell and Tissue Imaging Facility, Infrastructures en Biologie Santé et Agronomie (IBiSA), Paris F-75248, France.
authors.forename: Graça
authors.initials: G
authors.lastname: Raposo
mesh_terms: D002478:Cells, Cultured; D002853:Chromatography, Liquid; D004817:Epidermis; D055354:Exosomes / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D020460:Melanosomes / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010858:Pigmentation; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D014466:Ultraviolet Rays
chemical_list: D008543:Melanins; D012333:RNA, Messenger