title: A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells.
abstract: Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore.
authors.forename: Yogeswari
authors.initials: Y
authors.lastname: Chandran
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore.
authors.forename: Nam-Young
authors.initials: NY
authors.lastname: Kang
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Chemistry & MedChem Program, Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Samira
authors.initials: S
authors.lastname: Husen Alamudi
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore.
authors.forename: Srikanta
authors.initials: S
authors.lastname: Sahu
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Su
authors.affiliation: Department of Chemistry & MedChem Program, Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Jungyeol
authors.initials: J
authors.lastname: Lee
authors.affiliation: MRC Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, United Kingdom. Electronic address: mvendrel@staffmail.ed.ac.uk.
authors.forename: Marc
authors.initials: M
authors.lastname: Vendrell
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, #02-02 Helios, Singapore 138667, Singapore; Department of Chemistry & MedChem Program, Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. Electronic address: chmcyt@nus.edu.sg.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000032:analysis / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006575:Heterocyclic Compounds, 3-Ring / Q000032:analysis / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*
chemical_list: D001896:Boron Compounds; C000608314:CDy9 compound; D005456:Fluorescent Dyes; D006575:Heterocyclic Compounds, 3-Ring
keywords: Fluorescence imaging; Fluorescence-assisted cell sorting; Mouse embryonic stem cells; Single cell PCR; Three germ layers