title: Relative quantitation of multi-antennary N-glycan classes: combining PGC-LC-ESI-MS with exoglycosidase digestion.
abstract: In the search for N-glycan disease biomarkers current glycoanalytical methods may not be revealing a complete picture of precious samples, and we may be missing valuable structural information that fall outside analysis windows. We report a targeted strategy combining PGC-LC-ESI-MS with exoglycosidases to improve the relative quantitation of tri and tetra-antennary glycan classes.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia. nicki.packer@mq.edu.au.
authors.forename: J L
authors.initials: JL
authors.lastname: Abrahams
authors.forename: N H
authors.initials: NH
authors.lastname: Packer
authors.forename: M P
authors.initials: MP
authors.lastname: Campbell
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D011134:Polysaccharides; D006026:Glycoside Hydrolases