title: [Binding properties of components removable from dental base plate, analysed by Fourier-Transform Surface Plasmon Resonance (FT-SPR) method].
abstract: In parallel with the emergence of new dental materials the number of allergic diseases is continuously increasing. Extremely small quantities of the allergens are capable to inducing an allergic reaction. Therefore it is particularly important to examine these materials as antigens and investigate their binding properties to proteins (e.g. formaldehyde, methacrylic acid, benzoyl-peroxide...). The Fourier Transform Surface Plasmon Resonance Spectroscopy (FT-SPR) is a suitable examination method for this type of procedure. FT-SPR measurement is performed at a fixed angel of incident light, and reflectivity is measured over a range of wavelength in the near infrared. The advantages of this method are the outstanding sensitivity, the label-free detection capability and the possibility of the real-time testing procedure. Formaldehyde and methacrylic acid are among the most common dental allergens. In our study we examined these molecules by FT-SPR spectroscopy. The aim of this work was to investigate the suitability of this method to the detection of these materials, with special focuses on the analysis and evaluation concentration-dependent measurements. Different concentrations (0.01 %-0.2%) of formaldehyde and methacrylic acid solutions were measured. The individual spectra were measured for all of the solutions, and calibration curves were calculated for the materials for the possibility of the determination of an unknown concentration. The results confirmed that the method is theoretically capable to detect hundred-thousandths scale concentration-changes in the solution flowing above the SPR-chip. The concentration-dependent studies had proved that the method capable to measure directly these materials and can provide appropriate calibration for quantitative determination. These experiments show the broad applicability of the FT-SPR method, which can greatly facilitate the mapping and understanding of biomolecular interactions in the future.
authors.forename: József
authors.initials: J
authors.lastname: Bakó
authors.forename: Máté
authors.initials: M
authors.lastname: Kelemen
authors.forename: Melinda
authors.initials: M
authors.lastname: Szalóki
authors.forename: Géza
authors.initials: G
authors.lastname: Vitályos
authors.forename: Tünde
authors.initials: T
authors.lastname: Radics
authors.forename: Csaba
authors.initials: C
authors.lastname: Hegedüs
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D001585:Benzoyl Peroxide / Q000737:chemistry; D002138:Calibration; D003764:Dental Materials / Q000009:adverse effects / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde / Q000737:chemistry* / Q000276:immunology; D005583:Fourier Analysis; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
chemical_list: D000485:Allergens; D000941:Antigens; D003764:Dental Materials; D008689:Methacrylates; C008384:methacrylic acid; D005557:Formaldehyde; D001585:Benzoyl Peroxide