title: Effect of papain-based gel on type I collagen--spectroscopy applied for microstructural analysis.
abstract: Considering the improvement of biomaterials that facilitate atraumatic restorative techniques in dentistry, a papain-based gel can be used in the chemomechanical removal of decayed dental tissue. However, there is no information regarding the influence of this gel on the structure of sound collagen. The aim of the present study was to investigate the adsorption of a papain-based gel (Papacarie(TM)) to collagen and determine collagen integrity after treatment. A pilot study was first performed with 10 samples of type I collagen membrane obtained from bovine Achilles deep tendon to compare the influence of hydration (Milli-Q water) on infrared bands of collagen. In a further experiment, 10 samples of type I collagen membrane were used to evaluate the effects of Papacarie(TM) on the collagen microstructure. All analyses were performed using the attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR). The results demonstrated that the application of Papacarie(TM) does not lead to the degradation of collagen and this product can be safely used in minimally invasive dentistry. As the integrity of sound collagen is preserved after the application of the papain-based gel, this product is indicated for the selective removal of infected dentin, leaving the affected dentin intact and capable of re-mineralization.
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Zenildo Santos
authors.initials: ZS
authors.lastname: Silva Júnior
authors.affiliation: School of Dentistry, Nove de Julho University, São Paulo, SP, Brazil.
authors.forename: Sergio Brossi
authors.initials: SB
authors.lastname: Botta
authors.affiliation: Biomedical Engineering, Center of Engineering, Modeling and Applied Social Sciences, Federal University of ABC, Sao Bernardo do Campo, SP, Brazil.
authors.forename: Patricia Aparecida
authors.initials: PA
authors.lastname: Ana
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Cristiane Miranda
authors.initials: CM
authors.lastname: França
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Kristianne Porta Santos
authors.initials: KP
authors.lastname: Fernandes
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Raquel Agnelli
authors.initials: RA
authors.lastname: Mesquita-Ferrari
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Deana
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Sandra Kalil
authors.initials: SK
authors.lastname: Bussadori
mesh_terms: D000818:Animals; D002417:Cattle; D024042:Collagen Type I / Q000187:drug effects*; D005782:Gels / Q000494:pharmacology*; D008566:Membranes / Q000187:drug effects; D010206:Papain / Q000494:pharmacology*; D010865:Pilot Projects; D013055:Spectrophotometry, Infrared; D013710:Tendons / Q000187:drug effects
chemical_list: D024042:Collagen Type I; D005782:Gels; C509557:Papacarie; D010206:Papain