title: PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway.
abstract: The p53-inducible gene 3 (PIG3 or TP53I3) is a downstream gene of p53, which can be involved in the process of apoptosis induced by p53 via the production of reactive oxygen species (ROS). However, the functional significance of PIG3 in cancer remains to be determined. This aim of this study was to examine the mRNA and protein expression of PIG3 in papillary thyroid carcinoma (PTC) and normal thyroid tissues, assess the relationship between PIG3 expression and clinicopathological parameters in PTC and examine its role in the proliferation of PTC cell lines. The results showed that PIG3 was aberrantly overexpressed in the majority of specimens of PTC while the expression of p53 was lower in PTC compared with normal thyroid tissues. Anti-PIG3 immuno-reactivity positively correlated with TNM grade. In the PTC cell lines, PIG3 silencing using small interfering RNA (siRNAs) impaired their ability of proliferation and decreased the activity of the PI3K/AKT/PTEN pathway. The results suggested that PIG3 plays an oncogenic role in PTC via the regulation of the PI3K/AKT/PTEN pathway and support the exploration of PIG3 as a novel biomarker for patients with PTC.
authors.affiliation: Department of Oncologic Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Jinye
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Oncologic Surgery, The Affiliated Municipal Hospital, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Oncologic Surgery, The Affiliated Municipal Hospital, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Clinical Laboratory, Medical School, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Oncologic Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Qinyan
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Department of Clinical Laboratory, Medical School, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Oncologic Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D000077273:Thyroid Cancer, Papillary; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D011518:Proto-Oncogene Proteins; D017382:Reactive Oxygen Species; C411296:TP53I3 protein, human; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human