title: Recent advances and future applications of microfluidic live-cell microarrays.
abstract: Microfluidic live-cell microarrays show much promise as screening tools for biomedical research because they could shed light on key biological processes such as cell signaling and cell-to-cell and cell-to-substrate dynamic responses. While miniaturization reduces the need for expensive clinical grade reagents, the integration of functional components including micropumps, biosensors, actuators, mixers and gradient generators results in improved assay reliability, reproducibility and well-defined cell culture conditions. The present review addresses recent technological advances in microfluidic live-cell microarray technology with a special focus on the applications of microfluidic single-cell, multi-cell and 3D cell microarrays.
authors.affiliation: BioSensor Technologies, AIT Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. Electronic address: Mario.rothbauer.fl@ait.ac.at.
authors.forename: Mario
authors.initials: M
authors.lastname: Rothbauer
authors.affiliation: BioSensor Technologies, AIT Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. Electronic address: david.wartmann.fl@ait.ac.at.
authors.forename: David
authors.initials: D
authors.lastname: Wartmann
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Science, Vienna, Muthgasse 11, 1190 Vienna, Austria. Electronic address: verena.charwat@boku.ac.at.
authors.forename: Verena
authors.initials: V
authors.lastname: Charwat
authors.affiliation: BioSensor Technologies, AIT Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. Electronic address: peter.ertl@ait.ac.at.
authors.forename: Peter
authors.initials: P
authors.lastname: Ertl
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000639:trends; D006801:Humans; D056656:Lab-On-A-Chip Devices; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods / Q000639:trends; D009940:Organoids; D046888:Tissue Array Analysis / Q000379:methods / Q000639:trends
keywords: Body-on-a-chip; Lab-on-a-chip; Live-cell microarrays; Microfluidics; Organ-on-a-chip