title: Stereoselective Determination of Tebuconazole in Water and Zebrafish by Supercritical Fluid Chromatography Tandem Mass Spectrometry.
abstract: A simple and sensitive method for the enantioselective determination of tebuconazole enantiomers in water and zebrafish has been established using supercritical fluid chromatography (SFC)-MS/MS. The effects of the chiral stationary phases, mobile phase, auto back pressure regulator (ABPR) pressure, column temperature, flow rate of the mobile phase, and compensation pump solvent were evaluated. Finally, the optimal SFC-MS/MS working conditions were determined to include a CO2/MeOH mobile phase (87:13, v/v), 2.0 mL/min flow rate, 2200 psi ABPR, and 30 °C column temperature using a Chiralpak IA-3 chiral column under electrospray ionization positive mode. The modified QuEChERS method was applied to water and zebrafish samples. The mean recoveries for the tebuconazole enantiomers were 79.8-108.4% with RSDs ≤ 7.0% in both matrices. The LOQs ranged from 0.24 to 1.20 μg/kg. The developed analytical method was further validated by application to the analysis of authentic samples.
authors.affiliation: †Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.|§Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Zenglong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tao
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Xinglu
authors.initials: X
authors.lastname: Pan
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: XiXi
authors.initials: X
authors.lastname: Chen
authors.affiliation: §Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People's Republic of China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005659:Fungicides, Industrial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014230:Triazoles / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis; D015027:Zebrafish
chemical_list: D005659:Fungicides, Industrial; D014230:Triazoles; D014874:Water Pollutants, Chemical; D014867:Water; C087114:tebuconazole
keywords: chiral separation; supercritical fluid chromatography−tandem mass spectrometry; tebuconazole; zebrafish