title: [Clinical Implications on ZO-1 Gene Methylation in Myelodysplastic Syndrome Progression].
abstract: OBJECTIVE
  To investigate the clinical significance of ZO-1 gene methylation level in MDS progression in order to provide a theoretical basis for evaluating progrosis of MDS patients.
  
  
  METHODS
  The methylation specific PCR (MS-PCR) was performed to evaluate the ZO-1 gene methylation status in bone marrow samples of normal persons as control (NC). MDS and AML patients, the bisulfite sequencing PCR (BSP) was applied to detect the ZO-1 gene methylation status in serial bone marrow samples of MDS-RA, MDS-RAEB and AML stages of a MDS patients.
  
  
  RESULTS
  The possitive rate of ZO-1 gene methylation in samples of NC, MDS and AML patients displayed significant difference; in sample of NC group the positive of ZO-1 gene methylation was not observed, but the positive rate of ZO-1 gene methylation in samples of AML patients was highest (65.0%), the proportion of ZO-1 gene methylation in myeloid blast count of MDS/AML patients was higher (P=0.000). The serial samples in one MDS patient showed that along with progress of disease, the positive rate of ZO-1 gene methylation in MDS-RA, MDS-RAEB and AML stages was found to be obvious different (P=0.000), the positive rate of ZO-1 gene methylation in AML stage was highest (64.65%).
  
  
  CONCLUSIONS
  The high methylation in promoter region of ZO-1 gene has been found in MDS/AML patients, and along with clonal proliferation, the positive rate of ZO-1 methylation and positive froguency of methylation sites increase graduatly which suggests that the MDS progresses in a certain degree, and the ZO-1 gene methylation level may be used as an new indicator for monitoring desease progression from MDS to AML.
authors.affiliation: Department of Hematology, Chinese PLA's General Hospital, Beijing 100853, China.|Department of Clinical Laboratorial Examination, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Kang
authors.affiliation: Department of Hematology, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Hematology, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Mian-Yang
authors.initials: MY
authors.lastname: Li
authors.affiliation: Department of Hematology, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA's General Hospital, Beijing 100853, China.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA's General Hospital, Beijing 100853, China. E-mail: chunhuiliyu@yahoo.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D001853:Bone Marrow; D019175:DNA Methylation; D018450:Disease Progression; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D008745:Methylation; D009190:Myelodysplastic Syndromes; D016133:Polymerase Chain Reaction; D062826:Zonula Occludens-1 Protein
chemical_list: C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein