title: Clostridium amazonense sp. nov. an obliqately anaerobic bacterium isolated from a remote Amazonian community in Peru.
abstract: A strictly anaerobic Gram-stain positive, spore-forming, rod-shaped bacterium designated NE08V(T), was isolated from a fecal sample of an individual residing in a remote Amazonian community in Peru. Phylogenetic analysis based on the 16S rRNA gene sequence showed the organism belonged to the genus Clostridium and is most closely related to Clostridium vulturis (97.4% sequence similarity) and was further characterized using biochemical and chemotaxonomic methods. The major cellular fatty acids were anteiso C13:0 and C16:0 with a genomic DNA G + C content of 31.6 mol%. Fermentation products during growth with PYG were acetate and butyrate. Based on phylogenetic, phenotypic and chemotaxonomic information, strain NE08V was identified as representing a novel species of the genus Clostridium, for which the name Clostridium amazonense sp. nov. is proposed. The type strain is NE08V(T) (DSM 23598(T) = CCUG 59712(T)).
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: O'Neal
authors.affiliation: Department of Anthropology, University of Oklahoma, USA.
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: Obregón-Tito
authors.affiliation: Department of Anthropology, University of Oklahoma, USA.
authors.forename: Raul Y
authors.initials: RY
authors.lastname: Tito
authors.affiliation: Department of Anthropology, University of Oklahoma, USA.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Ozga
authors.affiliation: Department of Anthropology, University of Oklahoma, USA.
authors.forename: Susan I
authors.initials: SI
authors.lastname: Polo
authors.affiliation: Department of Anthropology, University of Oklahoma, USA.
authors.forename: Cecil M
authors.initials: CM
authors.lastname: Lewis
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, USA. Electronic address: paul.lawson@ou.edu.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D003013:Clostridium / Q000145:classification* / Q000302:isolation & purification*; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010568:Peru; D010802:Phylogeny; D044382:Population Groups; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012424:Rural Population; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA phylogeny; Clostridium amazonense sp. nov.; Indigenous community; Taxonomy