title: Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease.
abstract: BACKGROUND
  Previous studies have implicated a strong link between circulating plasma resistin and coronary artery disease (CAD). The aim of this study was to evaluate the differences in peripheral blood mononuclear cells (PBMC) resistin mRNA and its plasma protein concentrations between the patients with CAD of different clinical severity.
  
  
  METHODS
  This study included 33 healthy subjects as the control group (CG) and 77 patients requiring coronary angiography. Of the latter 30 was CAD negative whereas 47 were CAD positive [18 with stable angina pectoris (SAP) and 29 with acute coronary syndrome (ACS)]. Circulating resistin was measured by ELISA; PBMC resistin mRNA was determined by real-time PCR.
  
  
  RESULTS
  Resistin protein was significantly higher in the ACS group compared to the CG (P=0.001) and the CAD negative group (P=0.018). Resistin mRNA expression did not vary across the study groups, despite the positive correlation seen with plasma resistin (ρ=0.305, P=0.008). In patients, plasma resistin and PBMC resistin mRNA negatively correlated with HDL-C (ρ=-0.404, P<0.001 and ρ=-0.257, P=0.032, respectively). Furthermore, the highest plasma resistin tertile showed the lowest HDL-C (P=0.006). Plasma resistin was positively associated with serum creatinine (ρ=0.353, P=0.002).
  
  
  CONCLUSIONS
  Significant increase of plasma resistin in patients with ACS compared to CG and CAD negative patients was observed. Despite no change in PBMC resistin mRNA in different disease conditions a positive association between resistin mRNA and resistin plasma protein was evident. Both plasma resistin and PBMC resistin mRNA were negatively associated with plasma HDL-C, and plasma resistin positively with serum creatinine.
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Joksic
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
authors.forename: Miron
authors.initials: M
authors.lastname: Sopic
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Spasojevic-Kalimanovska
authors.affiliation: Institute for Cardiovascular Diseases, Clinical Center of Serbia, Belgrade, Serbia ; School of medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Kalimanovska-Ostric
authors.affiliation: Institute for Cardiovascular Diseases, Clinical Center of Serbia, Belgrade, Serbia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Andjelkovic
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
authors.forename: Zorana
authors.initials: Z
authors.lastname: Jelic-Ivanovic
mesh_terms: D000328:Adult; D000368:Aged; D008076:Cholesterol, HDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood* / Q000473:pathology; D003404:Creatinine / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood*; D052243:Resistin / Q000097:blood*; D012720:Severity of Illness Index
chemical_list: D008076:Cholesterol, HDL; C499624:RETN protein, human; D012333:RNA, Messenger; D052243:Resistin; D003404:Creatinine
keywords: acute coronary syndrome; coronary artery disease; gene expression; resistin, human