title: MiR-181a-5p inhibits cell proliferation and migration by targeting Kras in non-small cell lung cancer A549 cells.
abstract: MicroRNAs play important roles in carcinogenesis and tumor progress. Lung cancer is the leading cause of cancer mortality worldwide. In this study, the function of miR-181a-5p was investigated in non-small-cell lung cancer (NSCLC). Results showed that miR-181a-5p was significantly decreased in NSCLC tissues and cell lines. The proliferation and migration of A549 cells transfected with miR-181a-5p mimic was significantly inhibited. Luciferase activity assay results demonstrated that two binding sites of Kras could be directly targeted by miR-181a-5p. Furthermore, Kras was down-regulated by miR-181a-5p at both transcriptional and translational levels. SiRNA-mediated Kras down-regulation could mimic the effects of miR-181a-5p mimic in A549 cells. Our findings suggest that miR-181a-5p plays a potential role in tumor suppression by partially targeting Kras and has the potential therapeutic application in NSCLC patients.
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Ma
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Detao
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Wei
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Botao
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Shanghai Shines Pharmaceuticals Co., Ltd., Shanghai 200032, China.
authors.forename: Xintai
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Medicine, Shanghai Chest Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200030, China loujiatao@126.com yan.jin@siat.ac.cn jinyouxin@shu.edu.cn.
authors.forename: Jiatao
authors.initials: J
authors.lastname: Lou
authors.affiliation: Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, Shenzhen 518055, China loujiatao@126.com yan.jin@siat.ac.cn jinyouxin@shu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Life Sciences, Shanghai University, Shanghai 200444, China loujiatao@126.com yan.jin@siat.ac.cn jinyouxin@shu.edu.cn.
authors.forename: Youxin
authors.initials: Y
authors.lastname: Jin
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D058990:Molecular Targeted Therapy; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D014162:Transfection
chemical_list: C117307:KRAS protein, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D016283:Proto-Oncogene Proteins p21(ras)
keywords: miR-181a-5p; migration; non-small cell lung cancer; proliferation