title: MISTRAL: a transmission soft X-ray microscopy beamline for cryo nano-tomography of biological samples and magnetic domains imaging.
abstract: The performance of MISTRAL is reported, the soft X-ray transmission microscopy beamline at the ALBA light source (Barcelona, Spain) which is primarily dedicated to cryo soft X-ray tomography (cryo-SXT) for three-dimensional visualization of whole unstained cells at spatial resolutions down to 30 nm (half pitch). Short acquisition times allowing for high-throughput and correlative microscopy studies have promoted cryo-SXT as an emerging cellular imaging tool for structural cell biologists bridging the gap between optical and electron microscopy. In addition, the beamline offers the possibility of imaging magnetic domains in thin magnetic films that are illustrated here with an example.
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sorrentino
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Nicolás
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Valcárcel
authors.affiliation: Centro Nacional de Biotecnología - CSIC, Campus Cantoblanco, Madrid 28049, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Chichón
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Marc
authors.initials: M
authors.lastname: Rosanes
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Avila
authors.affiliation: Zeiss, Pleasanton, CA 94588, USA.
authors.forename: Andrei
authors.initials: A
authors.lastname: Tkachuk
authors.affiliation: Zeiss, Pleasanton, CA 94588, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Irwin
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Ferrer
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona 08290, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Pereiro
mesh_terms: D008280:Magnetics; D008853:Microscopy / Q000379:methods*; D014054:Tomography / Q000379:methods*; D014965:X-Rays
keywords: X-ray microscopy; correlative imaging; magnetic domains imaging; tomography