title: Time-resolved cell culture assay analyser (TReCCA Analyser) for the analysis of on-line data: data integration--sensor correction--time-resolved IC50 determination.
abstract: Time-resolved cell culture assays circumvent the need to set arbitrary end-points and reveal the dynamics of quality controlled experiments. However, they lead to the generation of large data sets, which can represent a complexity barrier to their use. We therefore developed the Time-Resolved Cell Culture Assay (TReCCA) Analyser program to perform standard cell assay analyses efficiently and make sophisticated in-depth analyses easily available. The functions of the program include data normalising and averaging, as well as smoothing and slope calculation, pin-pointing exact change time points. A time-resolved IC50/EC50 calculation provides a better understanding of drug toxicity over time and a more accurate drug to drug comparison. Finally the logarithmic sensor recalibration function, for sensors with an exponential calibration curve, homogenises the sensor output and enables the detection of low-scale changes. To illustrate the capabilities of the TReCCA Analyser, we performed on-line monitoring of dissolved oxygen in the culture media of the breast cancer cell line MCF-7 treated with different concentrations of the anti-cancer drug Cisplatin. The TReCCA Analyser is freely available at www.uni-heidelberg.de/fakultaeten/biowissenschaften/ipmb/biologie/woelfl/Research.html. By introducing the program, we hope to encourage more systematic use of time-resolved assays and lead researchers to fully exploit their data.
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany; Institute for Analytical Chemistry, Mannheim University of Applied Sciences, Mannheim, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Lochead
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Schessner
authors.affiliation: Institute for Analytical Chemistry, Mannheim University of Applied Sciences, Mannheim, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Werner
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Wölfl
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy; D002138:Calibration; D018929:Cell Culture Techniques / Q000379:methods; D002945:Cisplatin / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D010100:Oxygen / Q000737:chemistry; D011108:Polymers; D012815:Signal Processing, Computer-Assisted; D012984:Software
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D002945:Cisplatin; D010100:Oxygen