title: Establishment and Characterization of Two Novel Human Pancreatic Carcinoma Cell Lines.
abstract: BACKGROUND
  Pancreatic carcinoma (PC) is among the most lethal types of carcinomas worldwide. We aimed to establish well-defined PC cell lines in order to determine their resistance to chemotherapy.
  
  
  METHODS
  Cells cultured from the tumors of two patients were analyzed for xenograft formation, V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) and TP53 mutations, chemosensitivity, and mRNAs encoding rate-limiting enzymes that metabolize anticancer drugs.
  
  
  RESULTS
  The TYPK-1 and TYPK-2 cell lines were established from the lymph node of a locally advanced PC and from the ascites of a multi-metastatic and multi-chemoresistant PC, respectively. Each cell line generated tumors in nude mice. KRAS and TP53 mutations were detected in TYPK-1 but not TYPK-2 cells. TYPK-1 cells were resistant to gemcitabine, and TYPK-2 cells were resistant to oxaliplatin. The gemcitabine sensitivity of each cell line correlated with the expression of mRNAs encoding DCK and SLCAC29A1.
  
  
  CONCLUSIONS
  TYPK-1 and TYPK-2 cells may contribute to investigations of resistance to anticancer drugs.
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Hirano
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan okumura@med.u-toyama.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Okumura
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shimada
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Nagata
authors.affiliation: Department of Surgery and Science, University of Toyama. Sugitani, Toyama City, Toyama, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsukada
mesh_terms: D000369:Aged, 80 and over; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D009944:Organoplatinum Compounds / Q000494:pharmacology; D000077150:Oxaliplatin; D010190:Pancreatic Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D018631:ras Proteins / Q000235:genetics; D000093542:Gemcitabine
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D000077150:Oxaliplatin; D003841:Deoxycytidine; D018631:ras Proteins; D000093542:Gemcitabine
keywords: Pancreatic carcinoma; cell line characterization; chemotherapy