title: Alternative splicing, expression and cellular localization of Calneuron-1 in the rat and human brain.
abstract: Calneuron-1 and -2 are members of the neuronal calcium-binding protein family (nCaBP). They are transmembrane Calmodulin-like EF-hand Ca(2+)-sensors, and a function in the control of Golgi-to-plasma membrane vesicle trafficking has been assigned to both proteins. In this paper, we describe the distribution of Calneuron-1 in rat and human brains. We show that Calneuron-1 is ubiquitously expressed in all brain regions examined. The protein is most abundant in Purkinje cells of the cerebellum and principal neurons of the cortex and limbic brain whereas no expression in glial cells is apparent. In addition, we identify two novel splice isoforms of Calneuron-1 with extended N-termini. These isoforms are particular abundant in the cerebellum. Taken together, these data set grounds for a better understanding of the cellular function of Calneurons.
authors.affiliation: RG Neuroplasticity, Leibniz-Institute for Neurobiology, Magdeburg, Germany (JH, MMarunde, MMikhaylova, MRK)
authors.forename: Johannes
authors.initials: J
authors.lastname: Hradsky
authors.affiliation: Department of Psychiatry, Faculty of Medicine, Otto-von-Guericke University, Magdeburg, Germany (HGB).
authors.forename: Hans-Gert
authors.initials: HG
authors.lastname: Bernstein
authors.affiliation: RG Neuroplasticity, Leibniz-Institute for Neurobiology, Magdeburg, Germany (JH, MMarunde, MMikhaylova, MRK)
authors.forename: Monika
authors.initials: M
authors.lastname: Marunde
authors.affiliation: RG Neuroplasticity, Leibniz-Institute for Neurobiology, Magdeburg, Germany (JH, MMarunde, MMikhaylova, MRK)|Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (MMikhaylova)
authors.forename: Marina
authors.initials: M
authors.lastname: Mikhaylova
authors.affiliation: RG Neuroplasticity, Leibniz-Institute for Neurobiology, Magdeburg, Germany (JH, MMarunde, MMikhaylova, MRK)
authors.forename: Michael R
authors.initials: MR
authors.lastname: Kreutz
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics; D002147:Calmodulin / Q000032:analysis* / Q000235:genetics; D020832:EF Hand Motifs; D005091:Exons; D005260:Female; D015870:Gene Expression; D006801:Humans; D007438:Introns; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: C428830:CALN1 protein, human; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D020033:Protein Isoforms; C516555:calneuron I, rat
keywords: Calneuron; Golgi; cellular distribution; human brain; nCaBP; rat brain; splicing