title: Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry.
abstract: Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed.
authors.affiliation: ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. aurore.chevin@free.fr.
authors.forename: Aurore
authors.initials: A
authors.lastname: Chevin
authors.affiliation: Aix-Marseille Université, CNRS, AFMB UMR 7257, 13288 Marseille, France. bruno.coutard@afmb.univ-mrs.fr.
authors.forename: Bruno
authors.initials: B
authors.lastname: Coutard
authors.affiliation: ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. philippe.blanchard@anses.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.affiliation: Institute of Molecular and Cellular Pharmacology, IPMC, UMR6097 CNRS, 660 route des Lucioles, 06560 Valbonne, France. gay@ipmc.cnrs.fr.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Dabert-Gay
authors.affiliation: ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. magali.chabert@anses.fr.
authors.forename: Magali
authors.initials: M
authors.lastname: Ribière-Chabert
authors.affiliation: ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. richard.thiery@anses.fr.
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D002213:Capsid / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D016366:Open Reading Frames; D055503:Protein Multimerization; D012328:RNA Viruses / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D015678:Viral Structural Proteins / Q000032:analysis*
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins
keywords: MALDI-TOF/TOF; anti-pSP antibodies; chronic bee paralysis virus (CBPV); hypothetical structural protein (hSP); nano-HPLC; predicted structural protein (pSP); protein identification