title: Bluetongue virus in Oryx antelope (Oryx leucoryx) during the quarantine period in 2010 in Croatia.
abstract: Bluetongue (BT) is a viral infectious non‑contagious disease of domestic and wild ruminants. Insect species of the genus Culicoides (Diptera: Ceratopogonidae) serve as biological vectors that transmit bluetongue virus (BTV) to susceptible hosts. The infection is present in the Mediterranean region. Recently, it has also been reported in Central, Western, and Northern Europe where BTV‑8 was recognised as the causative serotype. In the meantime, BTV‑14 has appeared in the North‑Eastern part of Europe. In the present study, BTV serotype 16 (BTV‑16) was detected by virus neutralisation (VNT)‑assay and real‑time reverse transcription‑PCR (rRT‑PCR) in 1 antelope and BTV‑1 in 3 of 10 Oryx antelopes (Oryx leucoryx) imported in Croatia from the Sultanate of Oman. No BTV vectors were collected during the antelope quarantine on the Veliki Brijun Island. Also, no BTV antibodies were detected in sheep, cattle, and deer on the Island. Entomological studies did not reveal any new vector species that may have been introduced with the infected antelopes on their transportation. It was the first time that BTV was demonstrated in animals imported in Croatia. It involved BTV‑1, which had never been demonstrated before and BTV‑16, which had been previously recorded in domestic ruminants.
authors.affiliation: Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Bosnić
authors.forename: Relja
authors.initials: R
authors.lastname: Beck
authors.forename: Eddy
authors.initials: E
authors.lastname: Listeš
authors.forename: Ivana
authors.initials: I
authors.lastname: Lojkić
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savini
authors.forename: Besi
authors.initials: B
authors.lastname: Roić
mesh_terms: D000818:Animals; D000866:Antelopes / Q000821:virology*; D001820:Bluetongue virus / Q000302:isolation & purification*; D002519:Ceratopogonidae / Q000821:virology; D017523:Croatia; D011790:Quarantine / Q000662:veterinary; D013997:Time Factors