title: Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.
abstract: Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.
authors.affiliation: Electrical and Electronics Engineering Department, Bahcesehir University, Istanbul, 34349, Turkey.
authors.forename: Ayca Yalcin
authors.initials: AY
authors.lastname: Ozkumur
authors.affiliation: Department of Biological Engineering, Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.
authors.forename: Brittany A
authors.initials: BA
authors.lastname: Goods
authors.affiliation: Department of Chemical Engineering, Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.|The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.|The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, 02139, USA.
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Love
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry; D057134:Antibodies, Neutralizing / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry; D016466:CHO Cells; D002465:Cell Movement; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D015195:Drug Design; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000737:chemistry*; D015658:HIV Infections / Q000276:immunology* / Q000628:therapy; D015497:HIV-1; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D044085:Microfluidics; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D009500:Neutralization Tests; D012372:ROC Curve
chemical_list: D019380:Anti-HIV Agents; D057134:Antibodies, Neutralizing; D001688:Biological Products; D015483:HIV Antibodies
keywords: cell patterning; human immunodeficiency virus (HIV); living-cell arrays; neutralization