title: 5-Hydroxymethylcytosine Profiling in Human DNA.
abstract: Since its "re-discovery" in 2009, there has been significant interest in defining the genome-wide distribution of DNA marked by 5-hydroxymethylation at cytosine bases (5hmC). In recent years, technological advances have resulted in a multitude of unique strategies to map 5hmC across the human genome. Here we discuss the wide range of approaches available to map this modification and describe in detail the affinity based methods which result in the enrichment of 5hmC marked DNA for downstream analysis.
authors.affiliation: MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road, Edinburgh, EH4 2XU, UK.
authors.forename: John P
authors.initials: JP
authors.lastname: Thomson
authors.affiliation: The Centre for Individualized Medication, Linköping University Hospital, Linköping University, Linköping, SE-58185, Sweden.
authors.forename: Colm E
authors.initials: CE
authors.lastname: Nestor
authors.affiliation: MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road, Edinburgh, EH4 2XU, UK. richard.meehan@igmm.ed.ac.uk.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Meehan
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D016172:DNA Fingerprinting / Q000379:methods*; D057890:Epigenomics; D015894:Genome, Human; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D004247:DNA
keywords: 5-hydroxymethylcytosine; 5hmC; 5mC; DNA immunoprecipitation and enrichment; Epigenetics; HmeDIP