title: Conjugated Polythiophene for Rapid, Simple, and High-Throughput Screening of Antimicrobial Photosensitizers.
abstract: The cationic conjugated poly[3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-thiophene hydrochloride] (PMNT) has been developed for high-throughput screening of photodynamic antimicrobial chemotherapy photosensitizers (PSs). The bacterial number can be detected quantitatively by PMNT via various fluorescence quenching efficiencies. The photosensitized inactivation of bacteria is not efficient with ineffective PSs, and thus the bacteria grow exponentially and can be coated tightly by PMNT through electrostatic and hydrophobic interactions, resulting in aggregates and fluorescence quenching of PMNT, whereas, conversely, effective PSs lead to original and strong fluorescence of PMNT. This new platform of high-throughput screening is promising for discovering new PSs.
authors.affiliation: †School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, P. R. China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Li
authors.affiliation: §Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Niu
authors.affiliation: §Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Qi
authors.affiliation: §Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yuan
authors.affiliation: §Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Fan
authors.affiliation: §Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Hailong
authors.initials: H
authors.lastname: An
authors.affiliation: †School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, P. R. China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Yan
authors.affiliation: †School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, P. R. China.
authors.forename: Huanrong
authors.initials: H
authors.lastname: Li
authors.affiliation: §Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: †School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, P. R. China.|§Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin 300401, P. R. China.
authors.forename: Chengfen
authors.initials: C
authors.lastname: Xing
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage*; D002469:Cell Separation / Q000295:instrumentation; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D008027:Light; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects; D013876:Thiophenes / Q000737:chemistry* / Q000528:radiation effects; D016896:Treatment Outcome
chemical_list: D000890:Anti-Infective Agents; D017319:Photosensitizing Agents; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene
keywords: biosensor; conjugated polymers; fluorescence; high-throughput screening; photosensitizer