title: B7-H3 expression in children with asthma exacerbation.
abstract: BACKGROUND
  Recent studies have shown that B7-H3, a recently identified B7 family member, plays a critical role in the development of asthma.
  
  
  OBJECTIVE
  This study is to explore the expression of B7-H3 in children with asthma exacerbation.
  
  
  METHODS
  Twenty-one Chinese children with asthma exacerbation as well as 18 nonasthmatic control Chinese children were enrolled. B7-H3 level and cytokines (interferon [IFN]-γ, interleukin [IL]-4, and IL-10) determination were performed by enzyme-linked immunosorbent assay (ELISA) technique. Meanwhile, clinical parameters including laboratory findings, forced expiratory volume in one second (FEV(1)) and fractional exhaled nitric oxide were obtained.
  
  
  RESULTS
  Children with asthma exacerbation had significantly higher levels of B7-H3 than controls (4.46 ± 1.33 versus 3.42 ± 1.48 ng/mL; p = 0.027). Plasma IL-4 level was significantly higher in asthma exacerbation subjects than controls (157.98 ± 21.57 versus 121.92 ± 24.37 pg/mL; p < 0.0001), and IFN-γ level was significantly lower in asthma exacerbation subjects (292.73 ± 152.47 versus 421.78 ± 145.84 pg/mL; p = 0.0107). Level of B7-H3 in asthma exacerbation subjects with inhaled corticosteroid (ICS) treatment recently was significantly lower than subjects without ICS treatment (t = 2.706; p = 0.0136). Additionally, levels of B7-H3 decreased remarkably after prednisone treatment. Level of sB7-H3 in asthma exacerbation subjects was inversely correlated with level of IFN-γ (r(p) = -0.605; p = 0.005) after adjustment.
  
  
  CONCLUSIONS
  B7-H3 may play an important role in asthma exacerbation and was a useful clinical biomarker to evaluate asthma exacerbation.
authors.affiliation: Department of Respiratory Disease, Soochow University Affiliated Children's Hospital, Soochow University, Suzhou, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Chen
authors.forename: Guangbo
authors.initials: G
authors.lastname: Zhang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Yan
authors.forename: Canhong
authors.initials: C
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Meijuan
authors.initials: M
authors.lastname: Wang
authors.forename: Chuangli
authors.initials: C
authors.lastname: Hao
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
mesh_terms: D000367:Age Factors; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D001249:Asthma / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D060887:B7 Antigens / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006760:Hospitalization; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D011241:Prednisone / Q000627:therapeutic use
chemical_list: D018927:Anti-Asthmatic Agents; D060887:B7 Antigens; D015415:Biomarkers; C423237:CD276 protein, human; D016207:Cytokines; D011241:Prednisone