title: Proteomic Identification of Target Proteins of Thiodigalactoside in White Adipose Tissue from Diet-Induced Obese Rats.
abstract: Previously, galectin-1 (GAL1) was found to be up-regulated in obesity-prone subjects, suggesting that use of a GAL1 inhibitor could be a novel therapeutic approach for treatment of obesity. We evaluated thiodigalactoside (TDG) as a potent inhibitor of GAL1 and identified target proteins of TDG by performing comparative proteome analysis of white adipose tissue (WAT) from control and TDG-treated rats fed a high fat diet (HFD) using two dimensional gel electrophoresis (2-DE) combined with MALDI-TOF-MS. Thirty-two spots from a total of 356 matched spots showed differential expression between control and TDG-treated rats, as identified by peptide mass fingerprinting. These proteins were categorized into groups such as carbohydrate metabolism, tricarboxylic acid (TCA) cycle, signal transduction, cytoskeletal, and mitochondrial proteins based on functional analysis using Protein Annotation Through Evolutionary Relationship (PANTHER) and Database for Annotation, Visualization, Integrated Discovery (DAVID) classification. One of the most striking findings of this study was significant changes in Carbonic anhydrase 3 (CA3), Voltage-dependent anion channel 1 (VDAC1), phosphatidylethanolamine-binding protein 1 (PEBP1), annexin A2 (ANXA2) and lactate dehydrogenase A chain (LDHA) protein levels between WAT from control and TDG-treated groups. In addition, we confirmed increased expression of thermogenic proteins as well as reduced expression of lipogenic proteins in response to TDG treatment. These results suggest that TDG may effectively prevent obesity, and TDG-responsive proteins can be used as novel target proteins for obesity treatment.
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Korea. hilalparray@gmail.com.
authors.forename: Hilal Ahmad
authors.initials: HA
authors.lastname: Parray
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Korea. jwyun@daegu.ac.kr.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D017306:Annexin A2 / Q000235:genetics / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D037483:Galectin 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000080864:Lactate Dehydrogenase 5; D009765:Obesity / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013862:Thiogalactosides / Q000494:pharmacology*; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D017306:Annexin A2; D037483:Galectin 1; D007527:Isoenzymes; C550693:PEBP1 protein, rat; D049488:Phosphatidylethanolamine Binding Protein; D020543:Proteome; D013862:Thiogalactosides; C494764:Vdac1 protein, rat; C031803:thiodigalactoside; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5; D050995:Voltage-Dependent Anion Channel 1; D002256:Carbonic Anhydrases
keywords: galectin; obesity; proteome; thiodigalactoside; white adipose tissue