title: Complement C4a inhibits the secretion of hepatitis B virus screened by surface-enhanced laser desorption ionization time-flight mass spectrometry-based ProteinChip analysis.
abstract: OBJECTIVE
  Chronic hepatitis B (CHB) is a kind of chronic liver disease caused by persistent hepatitis B virus (HBV) infection. The study aims to seek the factors of host resistance to HBV and investigate their roles.
  
  
  METHODS
  Protein profiles of 58 healthy controls and 121 CHB patients were obtained by SELDI-TOF/MS. Predicted protein was validated by ELISA. Protein expression was evaluated by Western blot in the persistently HBV expressing cell line HepG2.2.15 and non-HBV expressing cell line HepG2. The level of HBV DNA was subsequently detected by quantitative real-time PCR in HepG2.2.15 cells with complement C4a treatment.
  
  
  RESULTS
  Significantly altered protein peaks were found through statistical analysis, and m/z 4300 was predicted by databases and successfully matched with the fragment of complement C4a. According to ELISA, serum complement C4a was found to be significantly lower in CHB patients compared with healthy controls (p < 0.001) and the area under receiver operating characteristics curve is 0.78. Furthermore, complement C4a showed lower expression in HepG2.2.5 cells and the secretion of HBV DNA was inhibited by complement C4a.
  
  
  CONCLUSIONS
  The present study implied the important role of complement C4a in inhibiting the HBV DNA secretion in CHB.
authors.affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Song
authors.affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Gui-Biao
authors.initials: GB
authors.lastname: Zhang
authors.affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Hu
authors.affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Lu
authors.affiliation: Institute of Liver Diseases, Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Yi-Fu
authors.initials: YF
authors.lastname: Yang
authors.affiliation: Research Center for Traditional Chinese Medicine and Systems Biology, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Yong-Yu
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Institute of Liver Diseases, Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China.
authors.forename: Yi-Yang
authors.initials: YY
authors.lastname: Hu
authors.affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, P. R. China.
authors.forename: Shi-Bing
authors.initials: SB
authors.lastname: Su
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015331:Cohort Studies; D015934:Complement C4a / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D015934:Complement C4a
keywords: Chronic hepatitis B; Complement C4a; Hepatitis B virus; SELDI-TOF/MS