title: Enhanced osteoconductivity of polyethersulphone nanofibres loaded with bioactive glass nanoparticles in in vitro and in vivo models.
abstract: OBJECTIVE
  There is growing need for new scaffold constructions for synthetic bone graft substitutes to repair large bone lesions. A very promising and important class of new implants for tissue engineering is based on three-dimensional scaffolds and bioceramics.
  
  
  METHODS
  In this study, after investigation of mechanical properties of polyethersulphone (PES) nanofibres, fabricated by electrospinning methodology and coated with bioactive glass (BG), cells of the MG-63 line were cultured on surfaces of these scaffolds. Their capacity to support MG-63 proliferation was also investigated in vitro by MTT assay. Osteoconductivity on these scaffolds was investigated by the common osteogenic markers alkaline phosphatase (ALP) activity, calcium mineral deposition and bone-related gene activation. Next, a bone reconstruction of rat critical-size defects model was evaluated using radiographic imaging analysis (digital mammography), computed tomography and histological examination.
  
  
  RESULTS
  In vitro results indicated that biocompatibility and osteogenic markers of MG-63 cells were significantly enhanced after coating PES with BG. Based on in vivo results, new bone formation in the defect site was enhanced in implanted rats in comparison with a control group. The highest reconstruction was observed in animals implanted with BG-coated nanofibres.
  
  
  CONCLUSIONS
  Osteoconductivity of PES nanofibres was markedly enhanced after coating them with BG, and introduction of this construct as new bone-graft substitute for bone loss and defects is indicated.
authors.affiliation: Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, 1997775555, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Faculty of Dentistry, Semmelweis University, Budapest, 1088, Hungary.
authors.forename: S
authors.initials: S
authors.lastname: Farhadian
authors.affiliation: Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, 1997775555, Iran.
authors.forename: F Jamshidi
authors.initials: FJ
authors.lastname: Adegani
authors.affiliation: Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, 1997775555, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Mirzaei
authors.affiliation: Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, 14115-111, Iran.
authors.forename: M Soufi
authors.initials: MS
authors.lastname: Zomorrod
authors.affiliation: Department of Medical Immunology, Faculty of Medical Science, Tatbiat Modares University, Tehran, 14115-111, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Langroudi
authors.affiliation: Materials Department, Engineering Faculty, Shahrekord University, Shahrekord, 8818634139, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Doostmohammadi
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, Tehran, 14155-6455, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Seyedjafari
authors.affiliation: Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, 14115-111, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000008:administration & dosage / Q000627:therapeutic use*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000627:therapeutic use*; D008297:Male; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D012886:Skull / Q000187:drug effects / Q000293:injuries* / Q000502:physiology / Q000648:ultrastructure; D013450:Sulfones / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D018786:Bone Substitutes; D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone