title: Saline wastewater treatment by Chlorella vulgaris with simultaneous algal lipid accumulation triggered by nitrate deficiency.
abstract: Chlorella vulgaris, a marine microalgae strain adaptable to 0-50 g L(-1) of salinity, was selected for studying the coupling system of saline wastewater treatment and lipid accumulation. The effect of total nitrogen (T N) concentration was investigated on algal growth, nutrients removal as well as lipid accumulation. The removal efficiencies of TN and total phosphorus (TP) were found to be 92.2-96.6% and over 99%, respectively, after a batch cultivation of 20 days. To illustrate the response of lipid accumulation to nutrients removal, C. vulgaris was further cultivated in the recycling experiment of tidal saline water within the photobioreactor. The lipid accumulation was triggered upon the almost depletion of nitrate (<5 mg L(-1)), till the final highest lipid content of 40%. The nitrogen conversion in the sequence of nitrate, nitrite, and then to ammonium in the effluents was finally integrated with previous discussions on metabolic pathways of algal cell under nitrogen deficiency.
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Qiao-Hui
authors.initials: QH
authors.lastname: Shen
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Gong
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Fang
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zi-Cheng
authors.initials: ZC
authors.lastname: Bi
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China. Electronic address: chenglihua@zju.edu.cn.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Cheng
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Xu
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Zhejiang 310027, PR China.
authors.forename: Huan-Lin
authors.initials: HL
authors.lastname: Chen
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D048408:Chlorella vulgaris / Q000378:metabolism*; D008055:Lipids / Q000502:physiology*; D053858:Metabolic Networks and Pathways / Q000502:physiology; D058086:Microalgae / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D058433:Photobioreactors; D012965:Sodium Chloride / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry*
chemical_list: D064751:Ammonium Compounds; D008055:Lipids; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D010758:Phosphorus; D012965:Sodium Chloride; D062065:Wastewater
keywords: Chlorella vulgaris; Lipid accumulation; Microalgae; Nitrogen deficiency; Saline wastewater