title: ErmF and ereD are responsible for erythromycin resistance in Riemerella anatipestifer.
abstract: To investigate the genetic basis of erythromycin resistance in Riemerella anatipestifer, the MIC to erythromycin of 79 R. anatipestifer isolates from China and one typed strain, ATCC11845, were evaluated. The results showed that 43 of 80 (53.8%) of the tested R. anatipestifer strains showed resistance to erythromycin, and 30 of 43 erythromycin-resistant R. anatipestifer strains carried ermF or ermFU with an MIC in the range of 32-2048 μg/ml, while the other 13 strains carrying the ereD gene exhibited an MIC of 4-16 μg/ml. Of 30 ermF + R. anatipestifer strains, 27 (90.0%) carried the ermFU gene which may have been derived from the CTnDOT-like element, while three other strains carried ermF from transposon Tn4351. Moreover, sequence analysis revealed that ermF, ermFU, and ereD were located within the multiresistance region of the R. anatipestifer genome.
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China; College of Animal Science and Veterinary Medicine, Shandong Agricultural University, No. 61, Daizong Road, Tai'an, 271018, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Xing
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Sun
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Cui
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Changcan
authors.initials: C
authors.lastname: Ou
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, No. 61, Daizong Road, Tai'an, 271018, China.
authors.forename: Weishan
authors.initials: W
authors.lastname: Chang
authors.affiliation: Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004372:Ducks; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics*; D060494:Riemerella / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004917:Erythromycin; D008780:Methyltransferases; C437393:ErmF protein, Bacteroides