title: Evaluation of Echinostoma liei worm, metacercaria and redia antigens for schistosomiasis control.
abstract: While chemotherepeutic drugs, such as praziquantel, oxamniquine and metrifonate, are currently considered safe and effective drugs for schistosomiasis treatment, reinfection occurs frequently after drug treatment. Thus, a vaccine is sought to provide long-term treatment. Antigens from worm, metacercaria and redia of Echinostoma liei (E. liei) were purified using CNBr-activated Sepharose column, then used for immunization of mice prior to infection with Schistosomiasis mansoni. Worm burden, hepatic and intestinal eggs and oogram count was significantly reduced and that was reflected in normalization of liver architecture. This referred to a significant increase in the tested immunoglobulin level (IgM, IgG1 and IgG2).
authors.affiliation: Zoology Dept., Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: G
authors.initials: G
authors.lastname: Abdel-Monaem
authors.affiliation: Zoology Dept., Faculty of Science, Cairo University, Giza, Egypt. Electronic address: alyaafarid@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Farid
authors.affiliation: Parasitology Dept., Theodore Bilharz Research Institute, Giza, Egypt.
authors.forename: I
authors.initials: I
authors.lastname: Rabia
authors.affiliation: Zoology Dept., Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: El-Amir
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D001700:Biomphalaria / Q000469:parasitology; D004449:Echinostoma / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007422:Intestines / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008297:Male; D051379:Mice; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000254:growth & development; D012555:Schistosomiasis mansoni / Q000517:prevention & control*; D014612:Vaccines
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D014612:Vaccines
keywords: Echinostoma liei; Immunoglobulins; Oogram; PZQ; Schistosomiasis; Worm burden