title: Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells.
abstract: The immune response relies on the migration of leukocytes and on their ability to stop in precise anatomical locations to fulfil their task. How leukocyte migration and function are coordinated is unknown. Here we show that in immature dendritic cells, which patrol their environment by engulfing extracellular material, cell migration and antigen capture are antagonistic. This antagonism results from transient enrichment of myosin IIA at the cell front, which disrupts the back-to-front gradient of the motor protein, slowing down locomotion but promoting antigen capture. We further highlight that myosin IIA enrichment at the cell front requires the MHC class II-associated invariant chain (Ii). Thus, by controlling myosin IIA localization, Ii imposes on dendritic cells an intermittent antigen capture behaviour that might facilitate environment patrolling. We propose that the requirement for myosin II in both cell migration and specific cell functions may provide a general mechanism for their coordination in time and space.
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Chabaud
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Mélina L
authors.initials: ML
authors.lastname: Heuzé
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Bretou
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Pablo
authors.initials: P
authors.lastname: Vargas
authors.affiliation: CNRS UMR144, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Maiuri
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Paola
authors.initials: P
authors.lastname: Solanes
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Maurin
authors.affiliation: CNRS UMR144, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Terriac
authors.affiliation: CNRS UMR144, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Maël
authors.initials: M
authors.lastname: Le Berre
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Danielle
authors.initials: D
authors.lastname: Lankar
authors.affiliation: CNRS UMR3215/Inserm U934, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Piolot
authors.affiliation: Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Adelstein
authors.affiliation: Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Yingfan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Sixt
authors.affiliation: National Jewish Health &University of Colorado, 1250 14th Street, Denver, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Jacobelli
authors.affiliation: CNRS UMR 7600, Université Pierre et Marie Curie, 4 Place Jussieu, 7600 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bénichou
authors.affiliation: 1] CNRS UMR 7600, Université Pierre et Marie Curie, 4 Place Jussieu, 7600 Paris, France [2] CNRS FRE 3231, Université Pierre et Marie Curie, 4 Place Jussieu, 75005 Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Voituriez
authors.affiliation: CNRS UMR144, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
authors.affiliation: Inserm U932, Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Lennon-Duménil
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D001854:Bone Marrow Cells; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D046210:Microfluidic Analytical Techniques; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism*
chemical_list: D000941:Antigens; D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C045084:invariant chain; D010047:Ovalbumin; D002403:Cathepsins; C028217:cathepsin S; D024462:Myosin Type II