title: A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.
abstract: A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. zhangfeng_njust@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. lishuangming1989@163.com.
authors.forename: Shuangming
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. caokang666@163.com.
authors.forename: Kang
authors.initials: K
authors.lastname: Cao
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. wpjshirley@163.com.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. suyan@njust.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. zhuxinhua@njust.edu.cn.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. wanying@njust.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
mesh_terms: D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D006801:Humans; D007610:Kallikreins / Q000097:blood*; D057230:Limit of Detection; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D015335:Molecular Probes; D017430:Prostate-Specific Antigen / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D004129:Dimethylpolysiloxanes; D015335:Molecular Probes; C013830:baysilon; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen
keywords: Love wave; PDMS; PSA; aptamer; biosensing; microfluidic; stem-loop probe