title: Cardiac engraftment of genetically-selected parthenogenetic stem cell-derived cardiomyocytes.
abstract: Parthenogenetic stem cells (PSCs) are a promising candidate donor for cell therapy applications. Similar to embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), PSCs exhibit self-renewing capacity and clonogenic proliferation in vitro. PSCs exhibit largely haploidentical genotype, and as such may constitute an attractive population for allogenic applications. In this study, PSCs isolated from transgenic mice carrying a cardiomyocyte-restricted reporter transgene to permit tracking of donor cells were genetically modified to carry a cardiomyocyte-restricted aminoglycoside phosphotransferase expression cassette (MHC-neor/pGK-hygror) to permit the generation of highly enriched cardiomyocyte cultures from spontaneously differentiating PSCs by simple selection with the neomycin analogue G148. Following engraftment into isogenic recipient hearts, the selected cardiomyocytes formed a functional syncytium with the host myocardium as evidenced by the presence of entrained intracellular calcium transients. These cells thus constitute a potential source of therapeutic donor cells.
authors.affiliation: Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, United States of America.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, United States of America; Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Rubart
authors.affiliation: Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, United States of America; Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Soonpaa
authors.affiliation: Institute of Pharmacology, University Medical Center Göttingen, Göttingen, Germany; DZHK (German Center for Cardiovascular Research), partner site Göttingen, Göttingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Didié
authors.affiliation: Institute of Pharmacology, University Medical Center Göttingen, Göttingen, Germany; DZHK (German Center for Cardiovascular Research), partner site Göttingen, Göttingen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Christalla
authors.affiliation: Institute of Pharmacology, University Medical Center Göttingen, Göttingen, Germany; DZHK (German Center for Cardiovascular Research), partner site Göttingen, Göttingen, Germany.
authors.forename: Wolfram-Hubertus
authors.initials: WH
authors.lastname: Zimmermann
authors.affiliation: Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, United States of America; Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America.
authors.forename: Loren J
authors.initials: LJ
authors.lastname: Field
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods; D018274:Electroporation; D005260:Female; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019868:Kanamycin Kinase / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology*; D010312:Parthenogenesis; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection; D019076:Transgenes
chemical_list: D049452:Green Fluorescent Proteins; D019868:Kanamycin Kinase