title: Phytoecdysteroids of the East Asian Caryophyllaceae.
abstract: BACKGROUND
  Occurrence of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3), 2-deoxy-20-hydroxyecdysone (4) has been analyzed in 64 species of the East Asian Caryophyllaceae.
  
  
  METHODS
  Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC). HPLC with a high-resolution mass spectrometry was performed on Shimadzu LCMS-IT-TOF (Japan) system equipped with a LC-20A Prominence liquid chromatograph, a photodiode array detector SPD-M20A and ion-trap/time-of-flight mass spectrometer.
  
  
  RESULTS
  New sources of phytoecdysteroids: Melandrium sachalinense and Melandrium firmum have been revealed. It is the 1(st) time that two has been identified in M. sachalinense and M. firmum; 1 in the species: Lychnis fulgens, Silene repens, Silene foliosa, Silene stenophylla, Silene jenisseensis and M. sachalinense; 3 in Lychnis cognata; 4 in L. fulgens, S. stenophylla and S. jenisseensis (the tribe Lychnideae, the subfamily Caryophylloideae). Ecdysteroid-negative taxa are Spergularia rubra of the tribe Sperguleae; species of the genera Minuartia, Honckenya, Eremogone, Arenaria, Moehringia, Pseudostellaria, Fimbripetalum, Stellaria and Cerastium of the tribe Alsineae; Scleranthus annuus of the tribe Sclerantheae, as well as the East Asian representatives of the genera Gypsophila, Psammophiliela, Dianthus and Saponaria of the tribe Diantheae; Oberna and Agrostemma of the tribe Lychnideae.
  
  
  CONCLUSIONS
  This investigation shows the most promising sources of ecdysteriods are species of genera Silene and Lychnis.
authors.affiliation: Laboratory of Plant Chemotaxonomy, G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Novozhilova
authors.affiliation: Laboratory of Comparative Biochemistry, A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Viacheslav
authors.initials: V
authors.lastname: Rybin
authors.affiliation: Laboratory of Plant Chemotaxonomy, G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Petr
authors.initials: P
authors.lastname: Gorovoy
authors.affiliation: Laboratory of Plant Chemotaxonomy, G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Gavrilenko
authors.affiliation: Far East Federal University, Vladivostok, Russia.
authors.forename: Roman
authors.initials: R
authors.lastname: Doudkin
keywords: Caryophyllaceae; ecdysteroids; high-performance liquid chromatography