title: Rapid determination of 1-deoxynojirimycin in Morus alba L. leaves by direct analysis in real time (DART) mass spectrometry.
abstract: A new method based on a Direct Analysis in Real Time (DART) ionization source coupled with triple quadrupole tandem mass spectrometry has been developed for rapid qualitative and quantitative analyses of 1-deoxynojirimycin (DNJ) in mulberry leaves. Two ions produced from DNJ, [M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162), are observed using DART-MS in the positive ion mode. The peak areas of the two selected ions monitoring (SIM) signals of ([M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162)) are integrated to determine the peak area for quantitative analyses. A reasonable linear regression equation is obtained in the range of 1.01 to 40.50 μg/mL, with a linear coefficient (R(2)) of 0.996. The limits of detection (LOD) and quantification (LOQ) of the method are 0.25 and 0.80 μg/mL, respectively. The range of recovery is shown to be 87.73-95.61%. The results derived from the developed DART-MS method are in good agreement with those from the conventional HPLC-FLD method. By contrast, DART-MS in SIM mode is a simple, rapid and high-throughput approach for the determination of the DNJ content in mulberry leaves. The present method is advantageous for the rapid screening of mulberry leaves containing high DNJ contents.
authors.affiliation: School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
authors.forename: Dong-Yang
authors.initials: DY
authors.lastname: Zhang
authors.affiliation: College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
authors.forename: Ze-Yu
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: ASPEC Technologies Limited, Room 1506, RunFenDeShangBldg A, No.60 An Li Lu, Chaoyang District, Beijing 100101, PR China.
authors.forename: Charles C
authors.initials: CC
authors.lastname: Liu
authors.affiliation: School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China; College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China. Electronic address: ghwu@just.edu.cn.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Wu
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis*; D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004566:Electrodes; D000431:Ethanol / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D007477:Ions; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D031625:Morus / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D005449:Fluorenes; D007477:Ions; D012997:Solvents; D017485:1-Deoxynojirimycin; D000431:Ethanol; D002725:Chloroform; C054007:1-(9-fluorenyl)methyl chloroformate; D009584:Nitrogen
keywords: DART–MS; DNJ; Mulberry leaves; Rapid determination; SIM