title: Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration.
abstract: We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at 70 frames per second, and the individual optical sections were assembled into a full 4D reconstruction of the beating heart using an optimized retrospective image registration algorithm. This imaging and reconstruction platform permitted us to visualize protein expression patterns at endogenous concentrations in zebrafish gene trap lines.
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125, USA ; Translational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA ; Equal contribution.
authors.forename: Vikas
authors.initials: V
authors.lastname: Trivedi
authors.affiliation: Translational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA ; Equal contribution.
authors.forename: Thai V
authors.initials: TV
authors.lastname: Truong
authors.affiliation: Translational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA.
authors.forename: Le A
authors.initials: le A
authors.lastname: Trinh
authors.affiliation: Translational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Holland
authors.affiliation: Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara CA 93111 USA ; Idiap Research Institute, 1920 Martigny, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Liebling
authors.affiliation: Translational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Fraser
keywords: (170.1420) Biology; (170.3010) Image reconstruction techniques; (180.0180) Microscopy; (190.0190) Nonlinear optics