title: [Expression Changes of Matrix Reconstitution-related Genes in Bone Marrow during 5-Fluorouracil-Induced Hemopoietic Injury and Their Significance].
abstract: OBJECTIVE
  To explore the expression change of the genes related with matrix reconstitution during the injury and reconstitution of murine bone marrow following treatment with 5-fluorouracil (5-FU).
  
  
  METHODS
  C57BL/6 mice received intraperitoneal injection of 5-FU (200 mg/kg), and peripheral blood cell counts were monitored at 3, 6, 9, 15, 21, 27 days after treatment. Bone marrow cells were harvested at these times for total RNA extraction using TRIzol. Reverse transcriptions in combination with real-time PCR were performed for detecting expression of genes related with matrix reconstitution, including ECM-1, MMP-2, MMP-3, MMP-13 and TIMP-1.
  
  
  RESULTS
  After injection of 5-FU, the numbers of three line cells in peripheral blood (i.e. RBC, WBC and platelets) decreased and then recovered with differential dynamics. Similarly, RT-qPCR revealed that all the 5 detected gene expressions were significantly up-regulated during the injury. The mRNA expression of MMP-2 reached to peak at day 3 while the other genes reached to peak at day 6. MMP-3 has a low expression when compared with others, but its expression increased significantly after injury.
  
  
  CONCLUSIONS
  In 5-FU induced hematopoietic injury and reconstitution model, matrix reconstitution-related genes may play an important role in hematopoietic reconstitution, but different genes play different roles at various time, and cooperate with each other for hematopoietic reconstitution.
authors.affiliation: Department of Anatomy, Qingdao University Medical College, Qingdao 266071, Shandong Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Ning
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Ya-Bin
authors.initials: YB
authors.lastname: Hu
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Shao
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China.
authors.forename: Yi-Qiang
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Qingdao University Medical College, Qingdao 266071, Shandong Province, China. E-mail :shenruowu@aliyun.com.
authors.forename: Ruo-Wu
authors.initials: RW
authors.lastname: Shen
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001773:Blood Cells / Q000473:pathology*; D001853:Bone Marrow; D001854:Bone Marrow Cells; D005109:Extracellular Matrix; D005472:Fluorouracil; D015870:Gene Expression; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D005472:Fluorouracil