title: HOW EXPRESSIONS OF CLAUDIN-1 AND MMP-2 IN RETINOBLASTOMA CORRELATE WITH HISTOLOGICAL DIFFERENTIATION AND OPTIC NERVE INVASION.
abstract: Retinoblastoma is a commonly seen and dangerous intraocular malignant tumor in infants. Studies have found that Claudin-1 and MMP-2, whose expressions may be connected, play roles in tissues of retinoblastoma. In this study we analyze and discuss changes of Claudin-1 and MMP-2 expressions, and the correlation between the expressions and retinoblastoma histological differentiation and optic nerve invasion. MaxVisionTM was applied to detect expressions of Claudin-1 and MMP-2 in 45 samples of retinoblastoma and 15 paraffin-embedded samples of normal retina. The correlation between Claudin-1 expression and MMP-2 expression was analyzed based on chi-squared test and Spearmans correlation test. Positive expressions of Claudin-1 in retinoblastoma were fewer than those in retina; higher positive expressions were found in differentiated tissues than in undifferentiated tissues; while compared to expressions in invasive optic nerves, Claudin-1 expressed more positively in optic nerves without invasion. As for MMP-2, its expressions were higher in retinoblastoma than in normal retina; undifferentiated tissues had higher positive expressions than differentiated tissues, which were not statistically significant; higher positive expressions were detected in invasive optic nerves. Thus, it could be concluded that the correlation between Claudin-1 expression and MMP-2 expression in retinoblastoma was negative. Expressions of Claudin-1 were positively related to histological differentiation and optic nerve invasion of retinoblastoma; while MMp-2 expression had negative correlation with histological differentiation and optic nerve invasion of retinoblastoma. Claudin-1 and MMP-2 played a negative role in the optic nerve invasion and tumor development of retinoblastoma.
authors.affiliation: Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: W C
authors.initials: WC
authors.lastname: Wan
authors.affiliation: Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Jin
authors.affiliation: Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Zheng
authors.affiliation: Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Zhang
authors.affiliation: Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D002454:Cell Differentiation; D002675:Child, Preschool; D062445:Claudin-1 / Q000032:analysis* / Q000502:physiology; D005134:Eye Neoplasms / Q000737:chemistry / Q000473:pathology*; D005136:Eye Proteins / Q000032:analysis* / Q000502:physiology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis* / Q000502:physiology; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis* / Q000502:physiology; D009900:Optic Nerve / Q000737:chemistry* / Q000473:pathology; D012175:Retinoblastoma / Q000737:chemistry / Q000473:pathology*
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; D005136:Eye Proteins; D009363:Neoplasm Proteins; D020778:Matrix Metalloproteinase 2