title: nextflu: real-time tracking of seasonal influenza virus evolution in humans.
abstract: Seasonal influenza viruses evolve rapidly, allowing them to evade immunity in their human hosts and reinfect previously infected individuals. Similarly, vaccines against seasonal influenza need to be updated frequently to protect against an evolving virus population. We have thus developed a processing pipeline and browser-based visualization that allows convenient exploration and analysis of the most recent influenza virus sequence data. This web-application displays a phylogenetic tree that can be decorated with additional information such as the viral genotype at specific sites, sampling location and derived statistics that have been shown to be predictive of future virus dynamics. In addition, mutation, genotype and clade frequency trajectories are calculated and displayed.
  
  
  METHODS
  Python and Javascript source code is freely available from https://github.com/blab/nextflu, while the web-application is live at http://nextflu.org.
  
  
  BACKGROUND
  tbedford@fredhutch.org.
authors.affiliation: Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany and.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Neher
authors.affiliation: Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Trevor
authors.initials: T
authors.lastname: Bedford
mesh_terms: D019143:Evolution, Molecular; D006801:Humans; D007251:Influenza, Human / Q000821:virology*; D009154:Mutation; D009975:Orthomyxoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012621:Seasons; D012984:Software