title: Histone hypoacetylation and increased histone deacetylases in peripheral blood mononuclear cells from patients with Graves' disease.
abstract: The objective of this study was to investigate histone modification patterns in peripheral blood mononuclear cells (PBMCs) of patients with Graves' disease (GD). Thirty GD patients and 20 healthy controls were enrolled in this study. Global histone H3/H4 acetylation levels of PBMCs in all subjects were detected by enzyme-linked immunosorbent assay. mRNA levels of histone-related chromatin modifier genes were measured by real-time quantitative reverse transcription-polymerase chain reaction. Global histone H4 acetylation level in PBMCs of GD patients was significantly decreased compared with controls (p=0.005). The mRNA expression of histone deacetylases HDAC1 and HDAC2 were significantly increased in PBMCs of GD patients compared with controls (p=0.004 and 0.018; respectively). No significant difference was observed either in SIRT1 or in HATs mRNA including p300, CREBBP between GD patients and controls (p>0.05). Our findings firstly suggested that histone acetylation modifications are aberrant in PBMCs of GD patients, possibly due to the deregulation of epigenetic modifier genes.
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Yan
authors.affiliation: Clinical Research Center, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Jiao-zhen
authors.initials: JZ
authors.lastname: Zhou
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, China. Electronic address: zhangjinan@hotmail.com.
authors.forename: Jin-an
authors.initials: JA
authors.lastname: Zhang
authors.affiliation: Clinical Research Center, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Cai
authors.affiliation: Clinical Research Center, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Clinical Research Center, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Clinical Research Center, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Fatuma-Said
authors.initials: FS
authors.lastname: Muhali
authors.affiliation: Clinical Research Center, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, China.
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Song
mesh_terms: D000107:Acetylation; D000328:Adult; D050882:CREB-Binding Protein / Q000097:blood / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D006111:Graves Disease / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D056284:Histone Deacetylase 1 / Q000097:blood / Q000235:genetics*; D056464:Histone Deacetylase 2 / Q000097:blood / Q000235:genetics*; D006657:Histones / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D056564:Sirtuin 1 / Q000097:blood / Q000235:genetics; D050880:p300-CBP Transcription Factors / Q000097:blood / Q000235:genetics
chemical_list: D006657:Histones; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; D050880:p300-CBP Transcription Factors; C447939:SIRT1 protein, human; D056564:Sirtuin 1; C110550:HDAC1 protein, human; C533423:HDAC2 protein, human; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2
keywords: Graves' disease (GD); Histone acetylation; Histone modifications; Peripheral blood mononuclear cells (PBMCs)