title: Aberrant expression of selenium-containing glutathione peroxidases in clear cell renal cell carcinomas.
abstract: OBJECTIVE
  To find putative diagnostic markers for clear cell renal cell carcinomas (ccRCC).
  
  
  METHODS
  Quantitative polymerase chain reaction (Q-PCR), bisulfite treatment, methylation-specific PCR, analysis on cBioPortal for Cancer Genomics.
  
  
  RESULTS
  We have found that expression of GPX1, GPX3, and GPX4 genes was decreased in ccRCC. We have shown that the number of alanine (GCG) repeats at the amino terminus of the GPX1 protein is variable. It was reported earlier that an allele that possess 5 alanine repeats is associated with the increased cancer risk. According to the obtained data, the allele with the 5 alanine repeats was also present in a group of healthy donors. Moreover, the frequency of alleles with repeats was similar among ccRCC patients and healthy individuals. We found that decreased expression of GPXs genes was not associated with promoter methylation. To provide other explanation, an analysis on the gene copy number was performed. We have found the heterozygous deletions for GPX1 gene, amplification for GPX3 gene, and no change in gene copy number for GPX4.
  
  
  CONCLUSIONS
  Our data support the hypothesis that GPX1, GPX3, and GPX4 genes may play a role in ccRCC cancerogenesis and therefore they might be considered as putative diagnostic markers for ccRCC.
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: E
authors.initials: E
authors.lastname: Rudenko
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Kondratov
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: G
authors.initials: G
authors.lastname: Gerashchenko
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: Y
authors.initials: Y
authors.lastname: Lapska
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: S
authors.initials: S
authors.lastname: Kravchenko
authors.affiliation: Institute of Gerontology, NAMS of Ukraine, Kyiv 04114, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Koliada
authors.affiliation: Institute of Urology, NAMS of Ukraine, Kyiv 04053, Ukraine.
authors.forename: S
authors.initials: S
authors.lastname: Vozianov
authors.affiliation: Institute of Urology, NAMS of Ukraine, Kyiv 04053, Ukraine.
authors.forename: Y
authors.initials: Y
authors.lastname: Zgonnyk
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03680, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Kashuba
mesh_terms: D063646:Carcinogenesis / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1
chemical_list: C486484:GPX3 protein, human; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human