title: Spleen function after preservation in a physiological solution.
abstract: BACKGROUND
  The purpose of this study was to evaluate the morphology and function of implanted autogenous spleen tissue after 24 h of preservation in a physiological solution.
  
  
  METHODS
  Thirty-five male rats were divided into seven groups (n = 5): group 1, without surgical procedure; group 2, total splenectomy; group 3, total splenectomy and immediate implant of autogenous spleen tissue; group 4, total splenectomy and preservation of the entire spleen in lactated Ringer solution at room temperature for 24 h, followed by spleen sectioning and implantation; group 5, total splenectomy, followed by spleen sectioning and preservation in lactated Ringer solution at room temperature for 24 h and subsequent implantation of the slices; group 6, total splenectomy and preservation of the entire spleen in lactated Ringer solution at 4°C for 24 h, followed by spleen sectioning and implantation; and group 7, total splenectomy, the spleen was sliced and preserved in lactate Ringer solution at 4°C for 24 h, followed by implantation of the slices. After 90 d, scintigraphic studies using sulfur colloid labeled with 99mTc of the liver, lungs, spleen, implants, and a blood clot were performed. Hematological (erythrogram, leukogram, and platelets) and histologic studies were carried out.
  
  
  RESULTS
  The autogenous splenic implants regenerated in all animals that received those implants preserved at 4°C and immediately after excision. The scintigraphic study showed a better phagocytic function in groups 1, 3, 6, and 7. No difference was observed in the hematological study.
  
  
  CONCLUSIONS
  Spleen tissue preserved in lactated Ringer solution at 4°C for 24 h maintains its vitality and capacity to recover hematological and phagocytic functions.
authors.affiliation: Department of Surgery, School of Medicine of the Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Argos Soares
authors.initials: AS
authors.lastname: de Matos Filho
authors.affiliation: Department of Surgery, School of Medicine of the Federal University of Minas Gerais, Belo Horizonte, Brazil. Electronic address: petroian@gmail.com.
authors.forename: Andy
authors.initials: A
authors.lastname: Petroianu
mesh_terms: D000818:Animals; D007552:Isotonic Solutions; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D000077325:Ringer's Lactate; D013154:Spleen / Q000637:transplantation*
chemical_list: D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D000077325:Ringer's Lactate
keywords: Autogenous splenic implant; Damage control; Lactated Ringer solution; Spleen; Trauma