title: Comparison of the single molecule activity distributions of recombinant and non-recombinant bovine intestinal alkaline phosphatase.
abstract: Single molecule assays were performed on bovine intestinal alkaline phosphatase and the recombinant enzyme expressed in Pichia pastoris using a capillary electrophoresis-based method. The catalytic rates for the bovine and recombinant enzymes were found to be 11,000±7000min(-1) (N=161) and 12,000±7000min(-1) (N=173), respectively. Mean catalytic rates and variances did not differ significantly between the enzyme from both sources. Furthermore, the distribution of catalytic rates were indistinguishable.
authors.affiliation: Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada. Electronic address: d.craig@uwinnipeg.ca.
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Craig
authors.affiliation: Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada.
authors.forename: Fiona
authors.initials: F
authors.lastname: Hanlon-Dearman
authors.affiliation: Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada.
authors.forename: Shailah
authors.initials: S
authors.lastname: Beaudry
authors.affiliation: Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada.
authors.forename: Kevin
authors.initials: K
authors.lastname: Shek
authors.affiliation: Chemistry Department, University of Winnipeg, Winnipeg, MB, Canada.
authors.forename: Steffany D
authors.initials: SD
authors.lastname: King
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary; D057075:Enzyme Assays; D010843:Pichia; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase; Bovine; Pichia pastoris; Recombinant; Single molecule