title: Nanosilver pathophysiology in earthworms: Transcriptional profiling of secretory proteins and the implication for the protein corona.
abstract: Previously we have identified lysenin as a key protein constituent of the secretome from Eisenia fetida coelomocytes and revealed its critical importance in priming interactions between the cells and the protein corona around nanosilver. As alterations of the protein environment can directly affect the corona composition, the extent to which nanoparticles influence the cells' protein secretion profile is of remarkable interest that has rarely acquired attention. Here, we have probed transcriptional responses of E. fetida coelomocytes to the representative nanosilver NM-300K (15 nm) in a time-dependent manner (2, 4, 8 and 24 h at a low-cytotoxic concentration), and examined the implication of the temporal changes in transcriptional profiles of secretory proteins with a particular reference to that of lysenin. NM-300K was accumulated in/at the cells and lysenin was, after transient induction, gradually suppressed over time indicating a negative feedback cycle. This may limit further enrichment of lysenin in the corona and thereby decrease the lysenin-assisted uptake of the nanoparticles. Other differentially expressed genes were those involved in metal stress (likewise in AgNO3-stressed cells) and in Toll-like receptor (TLR) signaling. This offers an intriguing perspective of the nanosilver pathophysiology in earthworms, in which the conserved pattern recognition receptor TLRs may play an effector role.
authors.affiliation: a iNANO Interdisciplinary Nanoscience Center, Aarhus University , Aarhus , Denmark .|b Department of Bioscience - Soil Fauna Ecology and Ecotoxicology , Aarhus University , Silkeborg , Denmark .
authors.forename: Yuya
authors.initials: Y
authors.lastname: Hayashi
authors.affiliation: a iNANO Interdisciplinary Nanoscience Center, Aarhus University , Aarhus , Denmark .
authors.forename: Teodora
authors.initials: T
authors.lastname: Miclaus
authors.affiliation: c Department of Immunology and Biotechnology , Clinical Center, University of Pécs , Pécs , Hungary , and.
authors.forename: Péter
authors.initials: P
authors.lastname: Engelmann
authors.affiliation: d Department of Public Health , Aarhus University , Aarhus , Denmark.
authors.forename: Herman
authors.initials: H
authors.lastname: Autrup
authors.affiliation: a iNANO Interdisciplinary Nanoscience Center, Aarhus University , Aarhus , Denmark .
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.affiliation: b Department of Bioscience - Soil Fauna Ecology and Ecotoxicology , Aarhus University , Silkeborg , Denmark .
authors.forename: Janeck J
authors.initials: JJ
authors.lastname: Scott-Fordsmand
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D000066970:Protein Corona / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012834:Silver / Q000737:chemistry / Q000633:toxicity*; D051193:Toll-Like Receptors / Q000378:metabolism; D014118:Toxins, Biological / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects
chemical_list: D000066970:Protein Corona; D011506:Proteins; D051193:Toll-Like Receptors; D014118:Toxins, Biological; C107040:lysenin; D012834:Silver
keywords: Biomolecular corona; NM-300K; coelomocytes; gene expression; toll-like receptors