title: MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells.
abstract: MED30 is an essential member of the mediator complex that forms a hub between transcriptional activators and RNA polymerase II. However, the expressions and roles of MED30 have been poorly characterized in cancer. In this study, we examined the functional roles of MED30 during gastric cancer progression. It was found that MED30 was overexpressed in gastric cancer tissues and cell lines. Moreover, MED30 overexpression increased the proliferation, migration, and invasion of gastric cancer cells, whereas MED30 knockdown inhibited these effects. Furthermore the knockdown significantly inhibited tumorigenicity in SCID mice. MED30 also promoted the expressions of genes related to epithelial-mesenchymal transition and induced a fibroblast-like morphology. This study shows MED30 has pathophysiological roles in the proliferation, migration, and invasion of gastric cancer cells and suggests that MED30 should be viewed as a potent therapeutic target for malignant gastric carcinoma.
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Yong Joo
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Busan, Republic of Korea; Medical Research Center for Ischemic Tissue Regeneration, Pusan National University, Busan, Republic of Korea.
authors.forename: Myoung-Eun
authors.initials: ME
authors.lastname: Han
authors.affiliation: Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Baek
authors.affiliation: Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea.
authors.forename: Seon-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Sae-Ock
authors.initials: SO
authors.lastname: Oh
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D056892:Mediator Complex / Q000378:metabolism*; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C570716:Med30 protein, mouse; D056892:Mediator Complex