title: Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system.
abstract: Hair follicle morphogenesis and regeneration depend on intensive but well-orchestrated interactions between epithelial and mesenchymal components. Therefore, an alternative strategy to reproduce the process of epithelial-mesenchymal interaction in vitro could use a 3D system containing appropriate cell populations. The 3D air-liquid culture system for reproducibly generating hair follicles from dissociated epithelial and dermal papilla (DP) cells combined with a collagen-chitosan scaffold is described in this study. Wnt-CM was prepared from the supernatant of Wnt1a-expressing bone marrow mesenchymal stem cells (BM-MSCs) that maintain the hair-inducing gene expression of DP cells. The collagen-chitosan scaffold cells (CCS cells) were constructed using a two-step method by inoculating the Wnt-CM-treated DP cells and epidermal (EP) cells into the CCS. The cells in the air-liquid culture formed dermal condensates and a proliferative cell layer in vitro. The CCS cells were able to induce hair regeneration in nude mice. The results demonstrate that Wnt-CM can maintain the hair induction ability of DP cells in expansion cultures, and this approach can be used for large-scale preparation of CCS cells in vitro to treat hair loss. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Hao
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Jiejie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Tong
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Ti
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Chen
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Central Laboratory, Hainan Branch, Chinese PLA General Hospital, Sanya, People's Republic of China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D018859:Hair Follicle / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D051155:Wnt1 Protein / Q000378:metabolism*
chemical_list: D051155:Wnt1 Protein; C495246:Wnt1 protein, mouse
keywords: Wingless-type mouse mammary tumour virus integration site; bone marrow mesenchymal stem cell; conditioned medium; dermal papilla; hair follicles; regeneration; three-dimensional; tissue engineering