title: Discrimination between native and Tn6010-associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by using a two-step strategy.
abstract: We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.
authors.affiliation: CHU Reims, Hôpital Robert Debré, Laboratoire de Bactériologie-Virologie-Hygiène, Reims, France UFR Médecine, EA 4687 ERA, SFR CAP-Santé, Université de Reims Champagne-Ardenne, Reims, France tguillard@chu-reims.fr.
authors.forename: Thomas
authors.initials: T
authors.lastname: Guillard
authors.affiliation: UFR Médecine, EA 4687 ERA, SFR CAP-Santé, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Lebreil
authors.affiliation: CHU Reims, Hôpital Robert Debré, Laboratoire d'Immunologie, Reims, France.
authors.forename: Lars Hestbjerg
authors.initials: LH
authors.lastname: Hansen
authors.affiliation: UFR Médecine, EA 4682, SFR CAP-Santé, Université de Reims Champagne-Ardenne, Reims, France Department of Environmental Science, Aarhus Univeristy, Roskilde, Denmark.
authors.forename: Aymric
authors.initials: A
authors.lastname: Kisserli
authors.affiliation: CHU Nancy, Laboratoire de Bactériologie, Nancy, France.
authors.forename: Sibel
authors.initials: S
authors.lastname: Berger
authors.affiliation: CHU Nancy, Laboratoire de Bactériologie, Nancy, France EA 7300 Stress Immunité Pathogènes, Université de Lorraine, Vandoeuvre-lès-Nancy, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Lozniewski
authors.affiliation: CHU Nancy, Laboratoire de Bactériologie, Nancy, France EA 7300 Stress Immunité Pathogènes, Université de Lorraine, Vandoeuvre-lès-Nancy, France.
authors.forename: Corentine
authors.initials: C
authors.lastname: Alauzet
authors.affiliation: CHU Reims, Hôpital Robert Debré, Laboratoire de Bactériologie-Virologie-Hygiène, Reims, France UFR Médecine, EA 4687 ERA, SFR CAP-Santé, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: de Champs
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects*; D007709:Klebsiella / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents