title: Diadenylate cyclase evaluation of ssDacA (SSU98_1483) in Streptococcus suis serotype 2.
abstract: Cyclic diadenosine monophosphate is a recently identified signaling molecule. It has been shown to play important roles in bacterial pathogenesis. SSU98_1483 (ssDacA), which is an ortholog of Listeria monocytogenes DacA, is a putative diadenylate cyclase in Streptococcus suis serotype 2. In this study, we determined the enzymatic activity of ssDacA in vitro using high-performance liquid chromatography and mass spectrometry. Our results showed that ssDacA was a diadenylate cyclase that converts ATP into cyclic diadenosine monophosphate in vitro. The diadenylate cyclase activity of ssDacA was dependent on divalent metal ions such as Mg(2+), Mn(2+), or Co(2+), and it is more active under basic pH than under acidic pH. The conserved RHR motif in ssDacA was essential for its enzymatic activity, and mutation in this motif abolished the diadenylate cyclase activity of ssDacA. These results indicate that ssDacA is a diadenylate cyclase, which synthesizes cyclic diadenosine monophosphate in Streptococcus suis serotype 2.
authors.affiliation: Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: B
authors.initials: B
authors.lastname: Du
authors.affiliation: Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China sunjhe@sjtu.edu.cn.
authors.forename: J H
authors.initials: JH
authors.lastname: Sun
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002413:Cations, Divalent; D003035:Cobalt / Q000737:chemistry / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000096:biosynthesis / Q000737:chemistry*; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008345:Manganese / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D065288:Serogroup; D017011:Streptococcus suis / Q000145:classification / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D015226:Dinucleoside Phosphates; D011994:Recombinant Proteins; C528998:cyclic diadenosine phosphate; D003035:Cobalt; D008345:Manganese; D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases; D008274:Magnesium