title: Novel real-time cell analysis platform for the dynamic monitoring of ionizing radiation effects on human tumor cell lines and primary fibroblasts.
abstract: Translational research in radiation oncology is important for the detection of adverse radiation effects, cellular responses, and radiation modifications, and may help to improve the outcome of radiation therapy in patients with cancer. The present study aimed to optimize and validate a real‑time label‑free assay for the dynamic monitoring of cellular responses to ionizing radiation. The xCELLigence system is an impedance‑based platform that provides continuous information on alterations in cell size, shape, adhesion, proliferation, and survival. In the present study, various malignant human primary fibroblast cells (U251, GBM2, MCF7, A549, HT‑29) were exposed to 0, 5 and 10 Gy of Cobalt60 radiation. As well as the xCELLigence system, cell survival and proliferation was evaluated using the following conventional end‑point cell‑based methods: Clonogenic, MTS, and lactate dehydrogenase assays, and apoptosis was detected by fluorescence‑activated cell sorting. The effects of ionizing radiation were detected for each cell line using impedance monitoring. The real‑time data correlated with the colony forming assay results. At low cell densities (1,000‑2,000 cells/well) the impedance‑based method was more accurate at monitoring dose‑dependent changes in the malignant human primary fibroblast cell lines, as compared with the end‑point assays. The results of the present study demonstrated that the xCELLigence system may be a reliable and rapid diagnostic method for the monitoring of dynamic cell behavior following radiation. In addition, the xCELLigence system may be used to investigate the cellular mechanisms underlying the radiation response, as well as the time‑dependent effects of radiation on cell proliferation and viability.
authors.affiliation: Avidin Ltd., Szeged H‑6726, Hungary.
authors.forename: Imola
authors.initials: I
authors.lastname: Mán
authors.affiliation: Avidin Ltd., Szeged H‑6726, Hungary.
authors.forename: Gábor J
authors.initials: GJ
authors.lastname: Szebeni
authors.affiliation: Department of Neurology, Faculty of Medicine, University of Szeged, Szeged H‑6725, Hungary.
authors.forename: Imola
authors.initials: I
authors.lastname: Plangár
authors.affiliation: Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Emilia R
authors.initials: ER
authors.lastname: Szabó
authors.affiliation: Institute of Surgical Research, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Tünde
authors.initials: T
authors.lastname: Tőkés
authors.affiliation: Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.affiliation: Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Nagy
authors.affiliation: Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Fekete
authors.affiliation: Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged H-6726, Hungary.
authors.forename: Roberta
authors.initials: R
authors.lastname: Fajka-Boja
authors.affiliation: Avidin Ltd., Szeged H‑6726, Hungary.
authors.forename: László G
authors.initials: LG
authors.lastname: Puskás
authors.affiliation: Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Hideghéty
authors.affiliation: Avidin Ltd., Szeged H‑6726, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Hackler
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D001681:Biological Assay / Q000295:instrumentation*; D002448:Cell Adhesion / Q000528:radiation effects; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D048430:Cell Shape / Q000528:radiation effects; D048429:Cell Size / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D017097:Electric Impedance; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009928:Organ Specificity; D061251:Primary Cell Culture; D013997:Time Factors
chemical_list: D007770:L-Lactate Dehydrogenase