title: The Association of Delayed Corneal Surface and Visual Recovery After Corneal Transplantation With Longer Donor Storage Time-A Prospective Analysis.
abstract: OBJECTIVE
  This study aimed to assess the influence of donor corneal storage time on endothelial cell count (ECC), corneal epithelial recovery, and visual rehabilitation after corneal transplantation in the first postoperative year.
  
  
  METHODS
  A collaborative prospective study involving a local eye bank and a tertiary ophthalmic unit was conducted.
  
  
  METHODS
  Donor cornea buttons were stored in Optisol-GS (Chiron Ophthalmics Inc, Irvine, Calif) storage media for a maximum of 14 days before transplantation. Before corneal distribution, the eye bank collected information on death-to-harvesting time, death-to-surgery time, donor central corneal thickness, and donor ECC at various time points. Subjects who underwent penetrating keratoplasty and endothelial keratoplasty were recruited and monitored for 1 year. Postoperative epithelial healing, visual acuity, ECC, and hospital stay were evaluated.
  
  
  RESULTS
  Thirty-one eyes of 31 patients completed the study. There was a significant positive correlation between donor storage time and epithelial healing (Spearman ρ = 0.39, P = 0.031). Faster epithelial healing was significantly correlated with posttransplantation visual improvements at months 1, 3, and 6 and shorter hospital stay (Spearman ρ = 0.74, P < 0.001). Mean ECC loss was 23.8% at 12 months posttransplantation. There was no significant correlation between storage time and ECC loss preoperatively and posttransplantation.
  
  
  CONCLUSIONS
  The duration of graft storage in Optisol-GS storage media up to 14 days had no significant effects on long-term visual acuity and ECC postoperatively. Shorter storage time had significant correlation with earlier epithelial healing and faster visual rehabilitation.
authors.affiliation: From the *Hospital Authority Ophthalmic Service, Hong Kong Eye Hospital, Hong Kong SAR, China; and †Hospital Authority Lion's Eye Bank, Hong Kong SAR, China.
authors.forename: Douglas K T
authors.initials: DK
authors.lastname: Lam
authors.forename: Tommy C Y
authors.initials: TC
authors.lastname: Chan
authors.forename: Janice S C
authors.initials: JS
authors.lastname: Wong
authors.forename: Galen Y
authors.initials: GY
authors.lastname: Chow
authors.forename: Victoria W Y
authors.initials: VW
authors.lastname: Wong