title: MALDI-mass spectrometry imaging identifies vitronectin as a common constituent of amyloid deposits.
abstract: Amyloids are pathological intra- and extracellular fibrillar aggregates of polypeptides with a cross-β-sheet structure and characteristic tinctorial properties. The amyloid deposits commonly enclose several non-fibrillar components of the extracellular matrix. Their potential to regulate the formation and aggregation process of amyloid fibrils is still poorly understood. For a better understanding of the role of the extracellular matrix in amyloidosis, it is essential to gain deeper insights into the composition of amyloid deposits. Here, we utilized matrix-assisted laser desorption and ionization mass spectrometry imaging to identify extracellular matrix compounds in amyloid deposits. Using this technique, we identified and determined the spatial distribution of vitronectin within AApoAI-, ALλ-, ATTR- and AIns amyloid deposits and, using immunohistochemistry, validated the spatial overlap of vitronectin with amyloids in 175 cases with diverse types of amyloid in several different tissues.
authors.affiliation: Department of Pathology (MW, SK, CR)
authors.forename: Martin
authors.initials: M
authors.lastname: Winter
authors.affiliation: Research Group Systematic Proteome Research, Institute of Experimental Medicine (AT|)Christian-Albrechts-University, Kiel, Germany (AT)
authors.forename: Andreas
authors.initials: A
authors.lastname: Tholey
authors.affiliation: Department of Pathology (MW, SK, CR)
authors.forename: Sandra
authors.initials: S
authors.lastname: Krüger
authors.affiliation: Department of Transplant Medicine, University Hospital Münster, Münster, Germany (HS).
authors.forename: Hartmut
authors.initials: H
authors.lastname: Schmidt
authors.affiliation: Department of Pathology (MW, SK, CR)
authors.forename: Christoph
authors.initials: C
authors.lastname: Röcken
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000682:Amyloid / Q000737:chemistry*; D000686:Amyloidosis / Q000473:pathology*; D001921:Brain / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007420:Intestine, Large / Q000473:pathology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding / Q000379:methods; D058225:Plaque, Amyloid / Q000473:pathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods; D019096:Vitronectin / Q000032:analysis*; D055815:Young Adult
chemical_list: D000682:Amyloid; D019096:Vitronectin
keywords: amyloid; formalin-fixed/paraffin-embedded tissue; immunohistochemistry; mass spectrometry imaging; matrix-assisted laser desorption/ionization; vitronectin