title: Neuroprotective effect of combined therapy with hyperbaric oxygen and madopar on 6-hydroxydopamine-induced Parkinson's disease in rats.
abstract: Parkinson's disease (PD) is a common movement disorder in the elderly. In the present study, we examined whether the combination of hyperbaric oxygen (HBO) and madopar therapy provided a neuroprotective effect on dopaminergic neurons in the substantia nigra using a rat model of PD. Rotational assessments revealed that both HBO and combination therapy significantly attenuated apomorphine-induced turning in PD rats. Our results indicated that the combination therapy increased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities and reduced the malondialdehyde (MDA) content in the SN. Furthermore, the combination therapy resulted in significant protection against the loss of neurons, and specifically tyrosine hydroxylase (TH)-positive neurons, in the SN and also alleviated the production of glial fibrillary acidic protein (GFAP). The levels of Bcl-2 were increased and Bax were decreased following the HBO or combination therapy. In brief, the neuroprotective effect of combined therapy with HBO and madopar against 6-OHDA-induced PD rats may rely on its ability to reduce oxidative stress and protect against Bax/Bcl-2-mediated apoptosis.
authors.affiliation: Department of Hyperbaric oxygen, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, PR China. Electronic address: panxron@163.com.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Hyperbaric oxygen, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, PR China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Hyperbaric oxygen, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Hyperbaric oxygen, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, PR China.
authors.forename: Haiming
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Hyperbaric oxygen, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, PR China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Fan
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001545:Benserazide / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D004338:Drug Combinations; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D007980:Levodopa / Q000627:therapeutic use*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D018384:Oxidative Stress; D016627:Oxidopamine / Q000627:therapeutic use*; D010300:Parkinson Disease / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017208:Rats, Wistar; D013239:Stereotyped Behavior; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D005904:Glial Fibrillary Acidic Protein; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; C005177:benserazide, levodopa drug combination; D007980:Levodopa; D008315:Malondialdehyde; D001545:Benserazide; D016627:Oxidopamine; D005979:Glutathione Peroxidase; D014446:Tyrosine 3-Monooxygenase; D013482:Superoxide Dismutase; D001058:Apomorphine
keywords: Apoptosis; HBO; Madopar; Oxidative stress; Parkinson’s disease; Substantia nigra