title: Evaluating the enantioselective distribution, degradation and excretion of epoxiconazole in mice following a single oral gavage.
abstract: 1. The enantiomeric enrichment or degradation of epoxiconazole has been reported in grape, soil, tubifex and mealworm beetle. But, little is known about its enantioselective behaviors in mammals. 2. To further understand differences in the distribution, degradation and excretion of epoxiconazole enantiomers in vivo, male CD-1 mice were selected as the test model to investigate the enantioselective behaviors after a single oral gavage. Mice were sacrificed after 1 h, 3 h, 7 h, 12 h, 24 h, 48 h, 72 h treatment, blood, tissues and excretions were collected for epoxiconazole analysis by LC-MS/MS. 3. On the Lux-Cellulose-1 chiral column, an enrichment of the second eluting (+)-epoxiconazole was generally observed, and feces and urine showed similar EF with major tissues. 4. To elucidate the potential role of intestinal bacterial flora in stereospecific degradation of epoxiconazole, mice fecal flora were cultured in vitro and incubated with epoxiconazole for 48 h. Results showed that (-)-epoxiconazole was preferentially degraded by intestinal bacterial. 5. These results may provide useful information for risk assessment of epoxiconazole on non-target animals.
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Dezhen
authors.initials: D
authors.lastname: Wang
authors.affiliation: b Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences , Beijing , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhu
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Nian
authors.initials: N
authors.lastname: Deng
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Applied Chemistry, China Agricultural University , Beijing , China and.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004852:Epoxy Compounds / Q000493:pharmacokinetics* / Q000494:pharmacology; D008297:Male; D051379:Mice; D013997:Time Factors; D014230:Triazoles / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D004852:Epoxy Compounds; D014230:Triazoles; C109476:epoxiconazole
keywords: Degradation; enantioselective; epoxiconazole; excretion; fecal flora; mice