title: [Importin-β1 plays a key role in the nucleocytoplasmic transportation process of MARVELD1].
abstract: While MARVEL domain-containing protein 1 (MARVELD1) is widely expressed in normal tissue, its expression is decreased in tumor tissue. Importin-β1 is a kind of nucleocytoplasmic transport protein, which participates in protein transportation between the nucleus and cytoplasm. It has been known that MARVELD1 binds importin-β1. To define the biological functions of the binding between MARVELD1 and importin-β1, confocal laser scanning microscopy was applied to confirm the distribution of MARVELD1 in the cells when importin-β1, is knocked down, and the results show that MARVELD1's concentration is decreased in the nucleus and elevated in the cytoplasm. GST-pull down and western blot were applied to test the binding affinity between the normal importin-β1, and several truncations of MARVELD1, and the binding affinity between the normal MARVELD1 and several truncations of importin-β1. These results show that there exist several binding points between MARVELD1 and importin-β1. Basing on these results one can come to a conclusion that importin-β1 mediates the non-classical nucleocytoplasmic transportation of MARVELD1. It was also found that knockdown of importin-β1, leads to an increase in the total density of MARVELD1. Considering the abnormally reduced expression of MARVELD1 in tumor tissue, the results of this study will help gaining a better understanding of the roles of MARVELD1 in the signal network systems during tumorigenesis in cancer cells.
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001 China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001 China.
authors.forename: M
authors.initials: M
authors.lastname: Shi
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001 China.|lilugene@hit.edu.cn.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D015398:Signal Transduction; D028961:beta Karyopherins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: C424166:KPNB1 protein, human; C542435:MARVELD1 protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D028961:beta Karyopherins
keywords: MARVELD1; importin-β1; nucleocytoplasmic transportation; protein-protein interaction