title: Reduction of start-up time through bioaugmentation process in microbial fuel cells using an isolate from dark fermentative spent media fed anode.
abstract: An electrochemically active bacteria Pseudomonas aeruginosa IIT BT SS1 was isolated from a dark fermentative spent media fed anode, and a bioaugmentation technique using the isolated strain was used to improve the start-up time of a microbial fuel cell (MFC). Higher volumetric current density and lower start-up time were observed with the augmented system MFC-PM (13.7 A/m(3)) when compared with mixed culture MFC-M (8.72 A/m(3)) during the initial phase. This enhanced performance in MFC-PM was possibly due to the improvement in electron transfer ability by the augmented strain. However, pure culture MFC-P showed maximum volumetric current density (17 A/m(3)) due to the inherent electrogenic properties of Pseudomonas sp. An electrochemical impedance spectroscopic (EIS) study, along with matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) analysis, supported the influence of isolated species in improving the MFC performance. The present study indicates that the bioaugmentation strategy using the isolated Pseudomonas sp. can be effectively utilized to decrease the start-up time of MFC.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India E-mail: ddas.iitkgp@gmail.com.
authors.forename: Soumya
authors.initials: S
authors.lastname: Pandit
authors.affiliation: Materials Science Centre, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Santimoy
authors.initials: S
authors.lastname: Khilari
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India E-mail: ddas.iitkgp@gmail.com.
authors.forename: Shantonu
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Civil Engineering, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: M M
authors.initials: MM
authors.lastname: Ghangrekar
authors.affiliation: Materials Science Centre, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Pradhan
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India E-mail: ddas.iitkgp@gmail.com.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Das
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D004566:Electrodes / Q000382:microbiology*; D004579:Electron Transport; D010084:Oxidation-Reduction; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003470:Culture Media