title: Role of Dendritic Cell-Specific ICAM-3-Grabbing Nonintegrin on Dendritic Cells in the Recognition of Hepatitis B Virus.
abstract: Dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) is an essential process for virus infection, such as HIV and hepatitis C, and plays a role in immune escape. However, the role of DC-SIGN in hepatitis B virus (HBV) infection is still unknown. The aim of this study was to investigate the role of DC-SIGN in mediating the maturation and activation of dendritic cells (DCs) when infected by HBV. Highly mannosylated HBV particles were obtained by treating HBV-producing HepG2.2.15 cells with the a-mannosidase I-inhibitor kifunensine. Highly mannosylated HBV or wild type HBV was added to infect the DCs of the DC-SIGN gene-silencing group and normal group, respectively. Then, the expression of CDla, CD80, CD83, CD86 and HLA-DR on DCs was detected by flow cytometry, the capacity of stimulating lymphocyte proliferation was tested by MTT assay, the level of IL-12p70 that was released by DCs was measured by enzyme-linked immunosorbent assay, and the expression of the proteins NF-κBp65 and p38 was detected by western blot. Both wild type and highly mannosylated HBV could promote DCs maturation and activation. However, the highly mannosylated HBV could promote DCs immune activation more strongly. The difference in the effect on DCs between the two types of HBV could be eliminated by DC-SIGN gene silencing. DC-SIGN can promote the maturation and activation of DCs when recognized HBV, but wild type HBV can escape recognition by DC-SIGN to a certain extent with the help of demannosylated modification, leading to defective DCs function and chronic HBV infection.
authors.affiliation: 1 Department of Infectious Disease, Huazhong University of Science and Technology , Wuhan, China .
authors.forename: Minxin
authors.initials: M
authors.lastname: Wang
authors.affiliation: 2 Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, China .
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zou
authors.affiliation: 1 Department of Infectious Disease, Huazhong University of Science and Technology , Wuhan, China .
authors.forename: Deying
authors.initials: D
authors.lastname: Tian
authors.affiliation: 1 Department of Infectious Disease, Huazhong University of Science and Technology , Wuhan, China .
authors.forename: Song
authors.initials: S
authors.lastname: Hu
authors.affiliation: 1 Department of Infectious Disease, Huazhong University of Science and Technology , Wuhan, China .
authors.forename: Libin
authors.initials: L
authors.lastname: Jiang
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000276:immunology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000032:analysis; D048051:p38 Mitogen-Activated Protein Kinases / Q000032:analysis; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D018664:Interleukin-12; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D051996:Transcription Factor RelA; D048051:p38 Mitogen-Activated Protein Kinases; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin