title: Responses of Bacterial Communities to Simulated Climate Changes in Alpine Meadow Soil of the Qinghai-Tibet Plateau.
abstract: The soil microbial community plays an important role in terrestrial carbon and nitrogen cycling. However, microbial responses to climate warming or cooling remain poorly understood, limiting our ability to predict the consequences of future climate changes. To address this issue, it is critical to identify microbes sensitive to climate change and key driving factors shifting microbial communities. In this study, alpine soil transplant experiments were conducted downward or upward along an elevation gradient between 3,200 and 3,800 m in the Qinghai-Tibet plateau to simulate climate warming or cooling. After a 2-year soil transplant experiment, soil bacterial communities were analyzed by pyrosequencing of 16S rRNA gene amplicons. The results showed that the transplanted soil bacterial communities became more similar to those in their destination sites and more different from those in their "home" sites. Warming led to increases in the relative abundances in Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria and decreases in Acidobacteria, Betaproteobacteria, and Deltaproteobacteria, while cooling had opposite effects on bacterial communities (symmetric response). Soil temperature and plant biomass contributed significantly to shaping the bacterial community structure. Overall, climate warming or cooling shifted the soil bacterial community structure mainly through species sorting, and such a shift might correlate to important biogeochemical processes such as greenhouse gas emissions. This study provides new insights into our understanding of soil bacterial community responses to climate warming and cooling.
authors.affiliation: Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chinese Academy of Sciences, and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Junpeng
authors.initials: J
authors.lastname: Rui
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, China lixz@cib.ac.cn wangsp@itpcas.ac.cn.
authors.forename: Shiping
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: An
authors.affiliation: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Wen-tso
authors.initials: WT
authors.lastname: Liu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, China.
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Lin
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China.
authors.forename: Yunfeng
authors.identifier: http://orcid.org/0000-0001-8274-6196
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China lixz@cib.ac.cn wangsp@itpcas.ac.cn.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Li
mesh_terms: D000531:Altitude; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D057231:Climate Change; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D018609:Tibet
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S