title: Serial Evaluation of Abdominal Fluid and Serum Amino-terminal pro-C-type Natriuretic Peptide in Dogs with Septic Peritonitis.
abstract: BACKGROUND
  Serum N-terminal pro-C-natriuretic peptide (NT-proCNP) has shown promise as a diagnostic biomarker for sepsis. Its sensitivity to detect dogs with septic peritonitis (SP) is reportedly low, perhaps attributable to the compartmentalization of NT-proCNP in the abdominal cavity.
  
  
  OBJECTIVE
  To evaluate the use of an ELISA for the measurement of NT-proCNP in canine abdominal fluid and to describe the peri-operative pattern of abdominal fluid and serum NT-proCNP concentrations in dogs with SP.
  
  
  METHODS
  Five client-owned dogs with nonseptic abdominal effusion of varying etiologies and 12 client-owned dogs with SP undergoing abdominal surgery and placement of a closed-suction abdominal drain (CSAD). Six dogs were included upon hospital admission; 6 were included the day after surgery.
  
  
  METHODS
  Prospective pilot study. A commercially available ELISA kit was analytically validated for use on canine abdominal fluid. The NT-proCNP concentrations were measured in the abdominal fluid of control dogs, and in serum and abdominal fluid of dogs with SP from admission for CSAD removal.
  
  
  RESULTS
  In dogs with SP, admission abdominal fluid NT-proCNP concentrations were lower than the concurrent serum concentrations (P = 0.031), and lower than control canine abdominal fluid concentrations (P = 0.015). Postoperatively, abdominal fluid NT-proCNP concentrations remained lower than serum concentrations (P < 0.050), except on day 4.
  
  
  CONCLUSIONS
  The ELISA kit was able to measure NT-proCNP in canine abdominal fluid. In dogs with SP, low serum NT-proCNP concentrations cannot be explained by abdominal compartmentalization.
authors.affiliation: Departments of Clinical Studies and Pathobiology, University of Guelph, Guelph, ON, Canada.
authors.forename: L V S
authors.initials: LV
authors.lastname: Guieu
authors.affiliation: Departments of Clinical Studies and Pathobiology, University of Guelph, Guelph, ON, Canada.
authors.forename: A M E
authors.initials: AM
authors.lastname: Bersenas
authors.affiliation: Departments of Clinical Studies and Pathobiology, University of Guelph, Guelph, ON, Canada.
authors.forename: M K
authors.initials: MK
authors.lastname: Holowaychuk
authors.affiliation: Departments of Clinical Studies and Pathobiology, University of Guelph, Guelph, ON, Canada.
authors.forename: B A
authors.initials: BA
authors.lastname: Brisson
authors.affiliation: Centre for Public Health and Zoonoses, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Weese
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008297:Male; D020098:Natriuretic Peptide, C-Type / Q000032:analysis* / Q000097:blood; D010538:Peritonitis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D010865:Pilot Projects; D011446:Prospective Studies; D018805:Sepsis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D015415:Biomarkers; D020098:Natriuretic Peptide, C-Type
keywords: Biomarkers; Canine; ELISA; Monitoring; Surgery