title: Purification of vandaterosides from Vanda teres (Orchidaceae) by stepwise gradient centrifugal partition chromatography.
abstract: Vandaterosides are polar glucosyloxybenzyl eucomate derivatives found in Vanda teres (Orchidaceae), which display biological activities that slow the skin ageing process. In order to obtain larger quantities to allow us to go further in the bioassays, the hydroalcoholic extract of aerial parts (leaves and stems) of V. teres were fractionated by centrifugal partition chromatography, combining isocratic, gradient, and dual elution modes. The first fractionation was performed on the extract maintained in the stationary phase as water saturated in butanol, while increasing the polarity of the mobile phase by changing the proportions of ethyl acetate/1-butanol/water, in order to obtain two enriched fractions. Vandateroside I was then purified by isocratic mode with ethyl acetate/ethanol/water (46:14:40), while vandateroside II was obtained by combining isocratic elution with ethyl acetate/isopropanol/water (30:20:50) followed by a multiple dual mode with ethyl acetate/ethanol/water (46:14:40). In this manner, hundreds of milligrams of vandateroside I and II were recovered from 10 g of V. teres extract.
authors.affiliation: Laboratory of Pharmacognosy and Bioactive Natural Products, UMR 7200, Faculty of Pharmacy, University of Strasbourg, Illkirch Graffenstaden, France.
authors.forename: Veronika
authors.initials: V
authors.lastname: Cakova
authors.affiliation: Laboratory of Pharmacognosy and Bioactive Natural Products, UMR 7200, Faculty of Pharmacy, University of Strasbourg, Illkirch Graffenstaden, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Urbain
authors.affiliation: Armen Instrument, ZI Kermelin, Saint-Avé, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Le Quéméner
authors.affiliation: Armen Instrument, ZI Kermelin, Saint-Avé, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Audo
authors.affiliation: LVMH Recherche, Saint Jean de Braye, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bonté
authors.affiliation: Laboratory of Pharmacognosy and Bioactive Natural Products, UMR 7200, Faculty of Pharmacy, University of Strasbourg, Illkirch Graffenstaden, France.
authors.forename: Annelise
authors.initials: A
authors.lastname: Lobstein
mesh_terms: D020001:1-Butanol; D000085:Acetates / Q000737:chemistry; D001593:Benzyl Compounds / Q000032:analysis*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D008293:Malates / Q000032:analysis* / Q000302:isolation & purification; D029595:Orchidaceae / Q000737:chemistry*; D010936:Plant Extracts; D028321:Plant Preparations / Q000032:analysis; D015203:Reproducibility of Results; D012997:Solvents; D014867:Water
chemical_list: D000085:Acetates; D001593:Benzyl Compounds; D005960:Glucosides; D008293:Malates; D010936:Plant Extracts; D028321:Plant Preparations; D012997:Solvents; C000608334:vandateroside I; C000608335:vandateroside II; D014867:Water; C007650:ethyl acetate; D020001:1-Butanol
keywords: Centrifugal partition chromatography; Orchids; Stepwise gradient; Vanda teres; Vandaterosides