title: siRNA transfection in larvae of the barnacle Amphibalanus amphitrite.
abstract: RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in 'double transfections', in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.
authors.affiliation: Environmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Science, No. 62, Fenghuang Road, Sanya City, Hainan Province 572000, China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: He
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
authors.forename: Yue Him
authors.initials: YH
authors.lastname: Wong
authors.affiliation: Environmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Environmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China KAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China boqianpy@ust.hk.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D000818:Animals; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008675:Metamorphosis, Biological; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D001468:Thoracica / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Amphibalanus amphitrite; Larval settlement; RNA interference; Transfection; p38 MAPK