title: Inhibition of autophagy induces IL-1β release from ARPE-19 cells via ROS mediated NLRP3 inflammasome activation under high glucose stress.
abstract: Autophagy plays an important role in the development of diabetic retinopathy (DR). Retinal pigment epithelial (RPE) cells are the main cells involved in DR, a process in which hyperglycemia plays a crucial role. This study was conducted to investigate the protective effect of autophagy against high glucose-induced inflammatory response in ARPE-19 cells and its underlying mechanism. In the present study we subjected ARPE-19 cells to high glucose stress and showed that ARPE-19 cells respond to high glucose with an increase in autophagy. 3-methyladenine (3-MA) inhibited occurrence of autophagy and it leaded to the accumulation of damaged-mitochondria-producing-ROS, and the activation of NLRP3 inflammasome, and subsequently, caused IL-1β secretion.
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: shihuanqi@sina.com.
authors.forename: Huanqi
authors.initials: H
authors.lastname: Shi
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: yiyi5337@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: wangxd139521@sina.com.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: 13945003007@163.com.
authors.forename: Ruishu
authors.initials: R
authors.lastname: Li
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: houwenwen0529@163.com.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Hou
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: biwenjiao1@163.com.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Bi
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, China. Electronic address: zhangxm667@163.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001343:Autophagy / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D017382:Reactive Oxygen Species; D005947:Glucose
keywords: Autophagy; Diabetic retinopathy; NLRP3 inflammasome; Retinal pigment epithelium