title: The effect of the bioactive sphingolipids S1P and C1P on multipotent stromal cells--new opportunities in regenerative medicine.
abstract: Sphingosine-1-phosphate (S1P) and ceramide-1-phosphate (C1P) belong to a family of bioactive sphingolipids that act as important extracellular signaling molecules and chemoattractants. This study investigated the influence of S1P and C1P on the morphology, proliferation activity and osteogenic properties of rat multipotent stromal cells derived from bone marrow (BMSCs) and subcutaneous adipose tissue (ASCs). We show that S1P and C1P can influence mesenchymal stem cells (MSCs), each in a different manner. S1P stimulation promoted the formation of cellular aggregates of BMSCs and ASCs, while C1P had an effect on the regular growth pattern and expanded intercellular connections, thereby increasing the proliferative activity. Although osteogenic differentiation of MSCs was enhanced by the addition of S1P, the effectiveness of osteoblast differentiation was more evident in BMSCs, particularly when biochemical and molecular marker levels were considered. The results of the functional osteogenic differentiation assay, which includes an evaluation of the efficiency of extracellular matrix mineralization (SEM-EDX), revealed the formation of numerous mineral aggregates in BMSC cultures stimulated with S1P. Our data demonstrated that in an appropriate combination, the bioactive sphingolipids S1P and C1P may find wide application in regenerative medicine, particularly in bone regeneration with the use of MSCs.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śmieszek
authors.forename: Marta
authors.initials: M
authors.lastname: Jeleń
authors.forename: Klaudia
authors.initials: K
authors.lastname: Chrząstek
authors.forename: Jakub
authors.initials: J
authors.lastname: Grzesiak
authors.forename: Justyna
authors.initials: J
authors.lastname: Meissner
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D002518:Ceramides / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D008246:Lysophospholipids / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D044968:Regenerative Medicine / Q000379:methods* / Q000639:trends; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002518:Ceramides; D008246:Lysophospholipids; C065576:ceramide 1-phosphate; D015675:Osteocalcin; D053495:Osteopontin; C060506:sphingosine 1-phosphate; D000469:Alkaline Phosphatase; D013110:Sphingosine