title: Efficacy of stannous, fluoride and their their combination in dentin erosion prevention in vitro.
abstract: The aim of this study was to compare the protective effects of solutions containing stannous (Sn), fluoride (F) and their combination in the prevention of dentin erosion. Forty bovine root dentin specimens (4'4'2 mm(3)) were prepared and randomly assigned to 4 groups (n = 10): SnCl2 (800 ppm/6.7 mM Sn), NaF (250 ppm/13 mM F), NaF/SnCl2 (800 ppm/6.7 mM Sn; 250 ppm/13 mM F), and deionized water (DIW) as a negative control. An acquired pellicle was formed on dentin samples by incubation in clarified, pooled, stimulated human saliva for 24 hours. The specimens were subjected to 5 daily cycles, each consisting of 5 of min demineralization (0.3%/15.6 mM citric acid, pH 2.6, 6'/day) and 60 min of re-mineralization in clarified human saliva. Thirty minutes after the 1st, 3rd and 5th demineralization episodes of each day, the specimens were treated with one of the test solutions for 2 min. Surface loss was measured via optical profilometry. Mixed-model ANOVA followed by Tukey's test were used for the statistical analysis. Sn, F, and their combination significantly reduced the dentin surface loss by 23%, 36%, and 60% compared with DIW, respectively. All groups were significantly different (p < 0.05). The combination of Sn and F significantly reduced the amount of dentin surface loss compared with all other groups. The F group also significantly reduced surface loss compared with Sn and DIW, followed by the Sn group, which showed significantly greater protection compared with the DIW control. The daily use of a combined fluoride and stannous solution is promising for preventing dentin erosion.
authors.affiliation: Department of Preventive and Community Dentistry, School of Dentistry, Indiana University, Indianapolis, IN, USA.
authors.forename: Amnah Abdullah
authors.initials: AA
authors.lastname: Algarni
authors.affiliation: Department of Preventive and Community Dentistry, School of Dentistry, Indiana University, Indianapolis, IN, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Lippert
authors.affiliation: Department of Preventive and Community Dentistry, School of Dentistry, Indiana University, Indianapolis, IN, USA.
authors.forename: Anderson Takeo
authors.initials: AT
authors.lastname: Hara
mesh_terms: D000818:Animals; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002417:Cattle; D003804:Dentin / Q000187:drug effects*; D004338:Drug Combinations; D006801:Humans; D011897:Random Allocation; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012969:Sodium Fluoride / Q000627:therapeutic use*; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D017971:Tin Compounds / Q000627:therapeutic use*; D014002:Tin Fluorides / Q000627:therapeutic use*; D014077:Tooth Erosion / Q000517:prevention & control*
chemical_list: D002327:Cariostatic Agents; D004338:Drug Combinations; D017971:Tin Compounds; D014002:Tin Fluorides; C023599:stannous chloride; D012969:Sodium Fluoride