title: Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda.
abstract: The type III secretion system (T3SS) of Edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. EseB, a component required for effector translocation, is reported to mediate autoaggregation of E. tarda. In this study, we demonstrate that EseB forms filamentous appendages on the surface of E. tarda and is required for biofilm formation by E. tarda in Dulbecco's modified Eagle's medium (DMEM). Biofilm formation by E. tarda in DMEM does not require FlhB, an essential component for assembling flagella. Dynamic analysis of EseB filament formation, autoaggregation, and biofilm formation shows that the formation of EseB filaments occurs prior to autoaggregation and biofilm formation. The addition of an EseB antibody to E. tarda cultures before bacterial autoaggregation prevents autoaggregation and biofilm formation in a dose-dependent manner, whereas the addition of the EseB antibody to E. tarda cultures in which biofilm is already formed does not destroy the biofilm. Therefore, EseB filament-mediated bacterial cell-cell interaction is a prerequisite for autoaggregation and biofilm formation.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan Province, China University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhi Peng
authors.initials: ZP
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Jin Fang
authors.initials: JF
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China.
authors.forename: Lu Yi
authors.initials: LY
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan Province, China.
authors.forename: Tiao Yi
authors.initials: TY
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China jsliu@ihb.ac.cn xieh@ihb.ac.cn.
authors.forename: Jia Shou
authors.initials: JS
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China jsliu@ihb.ac.cn xieh@ihb.ac.cn.
authors.forename: Hai Xia
authors.initials: HX
authors.lastname: Xie
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D020609:Edwardsiella tarda / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D000069296:Type III Secretion Systems / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D000069296:Type III Secretion Systems