title: Effect of Rhizoma paridis total saponins on apoptosis of colorectal cancer cells and imbalance of the JAK/STAT3 molecular pathway induced by IL-6 suppression.
abstract: We observed the influence of different concentrations of Rhizoma paridis total saponins (RPTS) on the apoptosis of colorectal cancer cells and explored the internal mechanism involved. We determined whether RPTS influences the interleukin-6 (IL-6)/Janus kinase (JAK)-signal transducer and activator of transcription-3 (STAT3) apoptosis molecular pathway and looked for colon cancer-related signal transduction pathways or targets inducing apoptosis. We also cultured SW480 colorectal cancer cells using different concentrations of RPTS (10, 20, 40, and 80 μg/ mL), and observed the effect of RPTS on SW480 cell morphology under a fluorescence inverted microscope. We detected serum IL-6 using the polymerase chain reaction and the expression of JAK-STAT3 protein by western blot. After treating SW480 with RPTS and Hoechst 33258 dyeing, we found that the typical apoptosis morphology had changed. Secretion of IL-6 in the serum decreased significantly (P < 0.05), and STAT3 levels were reduced. RPTS can significantly promote apoptosis in SW480 colorectal cancer cells. The mechanism may be that it suppresses the secretion of IL-6 and inhibits the IL-6/JAK-STAT3 protein signaling pathway.
authors.affiliation: The First Clinical College, Shandong University of Chinese Medicine, Changqing District, Jinan, Shandong Province, China.
authors.forename: W-J
authors.initials: WJ
authors.lastname: Teng
authors.affiliation: Oncology Department of Changshu Hospital of Traditional Chinese Medicine, Shixin District, Changshu, Jiangsu Province, China.
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.affiliation: Medical Oncology, Auxiliary Fuding Hospital, Fujian Traditional Chinese Medicine University of Fujian Province.
authors.forename: F-Y
authors.initials: FY
authors.lastname: Zhu
authors.affiliation: Department of Health Toxicology, Second Military Medical University, Yangpu District, Shanghai, China.
authors.forename: K
authors.initials: K
authors.lastname: Di
authors.affiliation: Cancer Center, Weifang Traditional Chinese Medicine Hospital, Kuiwen District, Weifang, Shandong Province, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Cancer Center, Weifang Traditional Chinese Medicine Hospital, Kuiwen District, Weifang, Shandong Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: The First Clinical College, Shandong University of Chinese Medicine, Changqing District, Jinan, Shandong Province, China.
authors.forename: X-J
authors.initials: XJ
authors.lastname: Cao
authors.affiliation: Cancer Center, Weifang Traditional Chinese Medicine Hospital, Kuiwen District, Weifang, Shandong Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.affiliation: Cancer Center, Weifang Traditional Chinese Medicine Hospital, Kuiwen District, Weifang, Shandong Province, China.
authors.forename: L-J
authors.initials: LJ
authors.lastname: Deng
authors.affiliation: Cancer Center, Weifang Traditional Chinese Medicine Hospital, Kuiwen District, Weifang, Shandong Province, China.
authors.forename: C-G
authors.initials: CG
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D053612:Janus Kinases / Q000096:biosynthesis* / Q000235:genetics; D010766:Phosphorylation; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D050796:STAT3 Transcription Factor / Q000096:biosynthesis / Q000235:genetics; D012503:Saponins / Q000008:administration & dosage* / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D010936:Plant Extracts; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D012503:Saponins; D053612:Janus Kinases