title: T, B, and NKT Cells in Systemic Inflammation in Obstructive Sleep Apnoea.
abstract: BACKGROUND
  Obstructive sleep apnoea syndrome (OSAS) brings risk of serious complications. The study objective was to assess elements of the cellular immune response in the course of OSAS.
  
  
  METHODS
  Peripheral blood (PB) lymphocytes: T, B, NK, NKT-like, Th, Tc, and HLA DR+ T cells were evaluated by flow cytometry of 48 OSA patients; the concentration of adiponectin, interleukin 1β, and TNFα was measured by ELISA method. The OSA complication score was developed and used for statistical analysis.
  
  
  RESULTS
  The proportion of B cells and Th/Tc ratio were significantly lower in the BP of OSA patients when compared with control subjects (median 7.9 versus 10.9%, 0.9 versus 1.5, p < 0.05). The proportion of Tc, NK, NKT-like, and HLADR positive T cells were elevated in OSA patients when compared with healthy subjects (36.4 versus 26.8, 15.5 versus 8.5, 5.7 versus 3.0, and 8.4 versus 4.5%, p < 0.05, resp.) and were more pronounced in patients with metabolic syndrome. The grade of OSA complication score correlated with systemic inflammation markers and the proportion of B cells. The value of adiponectin/BMI ratio correlated significantly with SpO2 (r = 0.31, p < 0.05), CRP (r = -0.35, p < 0.05), TNFα concentration (r = -0.36, p < 0.05), and proportion of B cells (r = 0.32, p < 0.05).
  
  
  CONCLUSIONS
  Lymphocytes B, Tc, NK, NKT-like, and adiponectin are involved in systemic immune response in OSA patients possibly predisposing them to cardiovascular and metabolic complications.
authors.affiliation: Department of Pneumonology and Allergology, Medical University of Warsaw, Ulica Banacha 1a, 02 097 Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Domagała-Kulawik
authors.affiliation: Department of Pneumonology and Allergology, Medical University of Warsaw, Ulica Banacha 1a, 02 097 Warsaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Osińska
authors.affiliation: Department of Pneumonology and Allergology, Medical University of Warsaw, Ulica Banacha 1a, 02 097 Warsaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Piechuta
authors.affiliation: Department of Pneumonology and Allergology, Medical University of Warsaw, Ulica Banacha 1a, 02 097 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Bielicki
authors.affiliation: Laboratory of Flow Cytometry, Medical Centre of Postgraduate Education, Ulica Marymoncka 99, 01 813 Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Skirecki
mesh_terms: D052242:Adiponectin / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000276:immunology*; D002097:C-Reactive Protein / Q000032:analysis; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D008875:Middle Aged; D055611:Natural Killer T-Cells / Q000276:immunology*; D020181:Sleep Apnea, Obstructive / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D052242:Adiponectin; D006684:HLA-DR Antigens; D053583:Interleukin-1beta; D002097:C-Reactive Protein