title: Glycopeptide Nanoconjugates Based on Multilayer Self-Assembly as an Antitumor Vaccine.
abstract: Antitumor vaccine, which is promising for tumor therapy, has been extensively studied. Some encouraging results of chemically synthetic vaccine designs based on the tumor-associated antigen mucin 1 have been achieved. However, some shortcomings such as low efficiency and difficult purification restrict their clinical application. To overcome these difficulties, we designed a novel antitumor vaccine of glycopeptide nanoconjugates based on the multilayer self-assembly through the interaction of positive and negative charges. This vaccine formed the spherical structure and effectively activated the macrophage in vitro. Besides, it also induced high titer of antibodies against mucin 1 glycopeptide. The induced antibodies could highly bind to the tumor cells and effectively kill them by activation of the complement dependent cytotoxicity complex. This novel strategy provides a new way for the development of simple and effective antitumor vaccine.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Liu
authors.forename: Zhan-Yi
authors.initials: ZY
authors.lastname: Sun
authors.forename: Pu-Guang
authors.initials: PG
authors.lastname: Chen
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Huang
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Zhao
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Chen
authors.affiliation: ‡National Engineering Research Center for Carbohydrate Synthesis, Jiangxi Normal University, Nanchang 330022, P. R. China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D019496:Cancer Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006020:Glycopeptides / Q000276:immunology*; D006801:Humans; D061986:MCF-7 Cells; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018396:Mucin-1 / Q000276:immunology*; D058726:Nanoconjugates / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D019496:Cancer Vaccines; D006020:Glycopeptides; D018396:Mucin-1; D058726:Nanoconjugates