title: The Dorsoventral Boundary of the Germinal Zone is a Specialized Niche for the Generation of Cortical Oligodendrocytes during a Restricted Temporal Window.
abstract: Oligodendrocyte precursor cells (OPCs) appear in the late embryonic brain, mature to become oligodendrocytes (OLs), and form myelin in the postnatal brain. Recently, it has been proposed that early-born OPCs derived from the ventral forebrain are eradicated postnatally and that late-born OLs predominate in the cortex of the adult mouse brain. However, intrinsic and extrinsic factors that specify the ability of self-renewing multipotent neural stem cells in the embryonic brain to generate cortical OL-lineage cells remain largely unknown. Using an inducible Cre/loxP system to permanently label Nestin- and Olig2-lineage cells, we identified that cortical OL-lineage cells start differentiating from neural stem cells within a restricted temporal window just prior to E16.5 through P10. We then showed, by means of electroporation of a Cre expression plasmid into the VZ/SVZ of E15.5 reporter mouse brains, that neural precursor cells in the dorsal VZ/SVZ are inhibited by Wnt signaling from contributing to cortical OLs in the adult brain. In contrast, neural precursor cells present in the dorsoventral boundary VZ/SVZ produce a significant amount of OLs in the adult cortex. Our results suggest that neural stem cells at this boundary are uniquely specialized to produce myelin-forming OLs in the cortex.
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.|Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Naruse
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.
authors.forename: Yugo
authors.initials: Y
authors.lastname: Ishino
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.|Current address: Department of Biology, Kyoto Prefectural University of Medicine, Kyoto 603-8334, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ono
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.|Current address: Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Takebayashi
authors.affiliation: Department of Physiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamaguchi
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ishizaki
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikenaka
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.|Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Okazaki 444-8787, Japan.|Department of Integrative Physiology, Shiga University of Medical Science, Otsu 520-2192, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Hitoshi
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002540:Cerebral Cortex / Q000254:growth & development* / Q000502:physiology*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000502:physiology*; D055153:Stem Cell Niche / Q000502:physiology*; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D000076104:Oligodendrocyte Transcription Factor 2; D051153:Wnt Proteins; C577485:Nes protein, mouse; C512773:Olig2 protein, mouse
keywords: FGF-2; Wnts; cortical oligodendrocyte; dorsoventral boundary; neural stem cell