title: Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town.
abstract: BACKGROUND
  Diarrheal disease and its complications remain a major cause of morbidity and mortality in children. The prevalence and antibiogram of E. coli as causative agents of diarrhea vary from region to region, and even within countries in the same geographical area.
  
  
  OBJECTIVE
  To determine the serotype and antimicrobial susceptibility of E. coli in children under-five years of age.
  
  
  METHODS
  A cross-sectional study was conducted among 422 children with diarrhea from December 2011 to February 2012. Identification of E. coli and antimicrobial susceptibility testing were done following standard procedures.
  
  
  RESULTS
  The overall isolation rate of E. coli was 48.3%. Poly 2 sero-groups, poly 3 sero-groups, poly 4 sero-groups and E. coli O157:H7 accounted for 80 (39.2%), 40 (19.6%), 25 (12.3%), and 59 (28.9%) of the isolates, respectively. Poly 2 sero-groups, constituting isolates belonging to enteropathogenic E. coli were the most commonly isolated serotypes. E. coli exhibited high levels of antimicrobial resistance to ampicillin (86.8%), tetracycline (76%) and cotrimoxazole (76%). Low levels of resistance to ciprofloxacin (6.9%) and norfloxacin (9.3%) were documented.
  
  
  CONCLUSIONS
  High prevalence of diarrheagenic E. coli compounded by alarming antimicrobial resistances is a serious public health problem. Regular determination of antibiogram and public education are recommended.
authors.affiliation: Department of Biology, Science College, Bahir Dar University.
authors.forename: Ayrikim
authors.initials: A
authors.lastname: Adugna
authors.affiliation: Department of Biology, Science College, Bahir Dar University.
authors.forename: Mulugeta
authors.initials: M
authors.lastname: Kibret
authors.affiliation: Department of Microbiology, Parasitology and Immunology, College of Medicine and Health Sciences, Bahir Dar University.
authors.forename: Bayeh
authors.initials: B
authors.lastname: Abera
authors.affiliation: Department of Biology, Science College, Bahir Dar University.
authors.forename: Endalkachew
authors.initials: E
authors.lastname: Nibret
authors.affiliation: Department of Biology, Science College, Bahir Dar University.
authors.forename: Melaku
authors.initials: M
authors.lastname: Adal
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology; D005002:Ethiopia / Q000453:epidemiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D065288:Serogroup
chemical_list: D000900:Anti-Bacterial Agents
keywords: E. coli; Ethiopia; antimicrobial susceptibility; diarrhea