title: Tetrahydrocurcumin exerts protective effect on vincristine induced neuropathy: Behavioral, biochemical, neurophysiological and histological evidence.
abstract: Hyperalgesia, allodynia, delayed motor nerve conduction velocity, oxidative stress and axonal damage are signs and symptoms of chemotherapy induced peripheral neuropathy (CIPN). Present treatment/preventive strategies of CIPN are futile and the neuropathy may even lead to discontinuation of chemotherapy. In this study, we evaluated the protective effect of tetrahydrocurcumin (THC) 40 and 80mg/kg in experimental vincristine induced neuropathy in rats. Hyperalgesia was assessed by hot plate (thermal), Randall-Selitto (mechanical) test, allodynia was assessed by cold plate (thermal) test, functional loss was measured by sciatic function index, nociception was evaluated by formalin test. Neurophysiological recordings were carried out to assess motor nerve conduction velocity. Total calcium levels, oxidative stress and TNF-α was measured in sciatic nerve tissue homogenate to assess neuropathy. Histopathological changes was observed on sciatic nerve to assess the protective effect of THC against the vincristine. Pregabalin was used as a standard in this study. Rats administered with THC at 80mg/kg significantly attenuated the vincristine induced neuropathic pain manifestations which may be due to its multiple actions including anti-nociceptive, anti-inflammatory, neuroprotective, calcium inhibitory and antioxidant effect. This study delineates that THC can be a promising candidate for the prevention of CIPN by chemotherapeutic agents.
authors.affiliation: Department of Pharmacology, PSG College of Pharmacy, Coimbatore 641004, Tamil Nadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Greeshma
authors.affiliation: Department of Pharmacology, PSG College of Pharmacy, Coimbatore 641004, Tamil Nadu, India.
authors.forename: K G
authors.initials: KG
authors.lastname: Prasanth
authors.affiliation: Department of Pharmacology, PSG College of Pharmacy, Coimbatore 641004, Tamil Nadu, India. Electronic address: ubbalaji@gmail.com.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Balaji
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003080:Cold Temperature; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005978:Glutathione / Q000378:metabolism; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009437:Neuralgia / Q000139:chemically induced / Q000517:prevention & control*; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014750:Vincristine / Q000633:toxicity
chemical_list: D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C096277:tetrahydrocurcumin; D009569:Nitric Oxide; D014750:Vincristine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D003474:Curcumin; D002118:Calcium
keywords: Allodynia; Anti-nociceptive; Hyperalgesia; Nerve conduction velocity; Pain; Sciatic nerve