title: Use of bergenin as an analytical marker for standardization of the polyherbal formulation containing Saxifraga ligulata.
abstract: BACKGROUND
  Bergenin is the principle constituent of the well-known medicinal plant Saxifraga ligulata. Bergenin has anti-inflammatory, antipyretic, antiviral, immunostimulant, antihyperglycemic, and antioxidant properties. In this study, the presence of bergenin in Saxifraga ligulata and the formulations was identified using high performance thin layer chromatograpy fingerprinting technique.
  
  
  OBJECTIVE
  To develop a novel quantitative method for the estimation of bergenin using high performance liquid chromatography.
  
  
  METHODS
  The compound was separated, characterised and quantified using authentic reference standard. The method was validated per ICH guidelines for the parameters of accuracy, precision, linearity, limit of detection, limit of quantification and robustness.
  
  
  RESULTS
  The method was found to be accurate, linear (r2 = 0.998) and precise (%RSD <2%). The limits of detection (0.001%) and quantification (0.002%) were found to be suitable for detection and quantification of bergenin in commercial formulations.
  
  
  CONCLUSIONS
  The developed methods are suitable for the quality control applications of Saxifraga ligulata containing formulations.
authors.affiliation: Formulation and Development, Research and Development Center, The Himalaya Drug Company, Makali, Bengaluru, India.
authors.forename: Hulikal Basavarajaiah
authors.initials: HB
authors.lastname: Pushpalatha
authors.affiliation: Department of Pharmaceutics, JSS College of Pharmacy, JSS University, Sri Shivarathreeswara Nagar, Mysore, Karnataka, India.
authors.forename: Kumar
authors.initials: K
authors.lastname: Pramod
authors.affiliation: Formulation and Development, Research and Development Center, The Himalaya Drug Company, Makali, Bengaluru, India.
authors.forename: Rajendran
authors.initials: R
authors.lastname: Devanathan
authors.affiliation: Formulation and Development, Research and Development Center, The Himalaya Drug Company, Makali, Bengaluru, India.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Sundaram
keywords: Bergenin; Saxifraga ligulata; dosage forms; high-performance liquid chromatography; high-performance thin layer chromatography