title: [S-Adenosylmethionine Inhibits Expression of Vascular Endothelial Growth Factor-C Protein and Cellular Proliferation in Gastric Cancer].
abstract: OBJECTIVE
  To explore inhibitory effects of S-adenosylmethionine on vascular endothelial growth factor-C (VEGF-C) protein and cellular proliferation in gastric cancer by regulating methylation status of VEGF-C promoter.
  
  
  METHODS
  MTT analyses and nude mice model were employed to examine the effects of S- adenosylmethionine on inhibiting gastric cancer growth in vitro and in vivo. The protein expression of VEGF-C in gastric cancer cells was assessed by Western blot. The methylation status of VEGF-C promoter was assessed by bisulfite genomic DNA sequencing analysis.
  
  
  RESULTS
  VEGF-C promoter was hypomethylated in MGC803 and SGC7901. The treatment of S-adenosylmethionine resulted in a heavy hypermethylation of VEGF-C promoter, which consequently down regulated protein level of VEGF-C. S-adenosylmethionine effectively inhibited the growth of gastric cancer cells in vitro and in vivo (P<0. 05).
  
  
  CONCLUSIONS
  S-adenosylmethionine can effectively reverse DNA hypomethylation on VEGF-C promoter which down-regulates VEGF-C protein expression and inhibit gastric cancer growth.
authors.forename: Yong-bin
authors.initials: YB
authors.lastname: Zhang
authors.forename: Ming-xu
authors.initials: MX
authors.lastname: Da
authors.forename: Ji-bin
authors.initials: JB
authors.lastname: Yao
authors.forename: Yao-xing
authors.initials: YX
authors.lastname: Duan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D019175:DNA Methylation; D015536:Down-Regulation; D051379:Mice; D008819:Mice, Nude; D011401:Promoter Regions, Genetic; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
chemical_list: D042582:Vascular Endothelial Growth Factor C; D012436:S-Adenosylmethionine