title: The essential function of Rrs1 in ribosome biogenesis is conserved in budding and fission yeasts.
abstract: The Rrs1 protein plays an essential role in the biogenesis of 60S ribosomal subunits in budding yeast (Saccharomyces cerevisiae). Here, we examined whether the fission yeast (Schizosaccharomyces pombe) homologue of Rrs1 also plays a role in ribosome biogenesis. To this end, we constructed two temperature-sensitive fission yeast strains, rrs1-D14/22G and rrs1-L51P, which had amino acid substitutions corresponding to those of the previously characterized budding yeast rrs1-84 (D22/30G) and rrs1-124 (L61P) strains, respectively. The fission yeast mutants exhibited severe defects in growth and 60S ribosomal subunit biogenesis at high temperatures. In addition, expression of the Rrs1 protein of fission yeast suppressed the growth defects of the budding yeast rrs1 mutants at high temperatures. Yeast two-hybrid analyses revealed that the interactions of Rrs1 with the Rfp2 and Ebp2 proteins were conserved in budding and fission yeasts. These results suggest that the essential function of Rrs1 in ribosome biogenesis may be conserved in budding and fission yeasts.
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Kun
authors.initials: K
authors.lastname: Wan
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Kawara
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kume
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Yabuki
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.|National Research Institute of Brewing, Higashi-Hiroshima, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Goshima
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kitamura
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ueno
authors.affiliation: National Research Institute of Brewing, Higashi-Hiroshima, Japan.
authors.forename: Muneyoshi
authors.initials: M
authors.lastname: Kanai
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.|Asahi-shuzo Sake Brewing Co. Ltd, Niigata, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Funato
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuta
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins
keywords: Rrs1; budding yeast; fission yeast; ribosome biogenesis