title: Helicobacter pylori: correlation of the virulence marker iceA allele with clinical outcome in a high prevalence area.
abstract: The association of Helicobacter pylori virulence marker 'induced by contact with epithelium A' (iceA) allele types was determined in H. pylori-related diseases and virulence markers. Gastric biopsies were obtained at EGD from patients for culture, histopathology and polymerase chain reaction (PCR) for iceA types, cagA and vacA alleles. Two hundred and eighty-four H. pylori isolates were examined. iceA type 1 was positive in 177 (62%) and iceA type 2 in 158 (56%). In iceA type 2, gastric ulcer was present in 34 (21%) (P < 0.001) and carcinoma in 28 (25%) (P = 0.002), compared to nine (8%) and 2 (2%) in iceA type 2-negative cases. For iceA type 2, 139 (88%) were associated with chronic active gastritis compared to 95 (75%) (P = 0.006) in iceA type 2-negative. H. pylori cagA was positive in 101 (64%) iceA type 2 strains compared to 57 (45%) in negative strains (P = 0.002). H. pylori iceA type 2 was dominant and associated with cagA, chronic active inflammation, gastric ulcer and carcinoma.
authors.forename: J
authors.initials: J
authors.lastname: Yakoob
authors.forename: Z
authors.initials: Z
authors.lastname: Abbas
authors.forename: R
authors.initials: R
authors.lastname: Khan
authors.forename: S A
authors.initials: SA
authors.lastname: Salim
authors.forename: A
authors.initials: A
authors.lastname: Abrar
authors.forename: S
authors.initials: S
authors.lastname: Awan
authors.forename: Z
authors.initials: Z
authors.lastname: Ahmad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology; D013276:Stomach Ulcer / Q000382:microbiology / Q000473:pathology; D016896:Treatment Outcome; D014774:Virulence / Q000235:genetics; D055815:Young Adult
chemical_list: D001425:Bacterial Outer Membrane Proteins; C063113:ice nucleation protein