title: FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus.
abstract: Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion ability in this study was screened out and designated A77. Molecular analysis showed that a 414-bp sequence flanking mini-Tn10 in A77 had the highest identity (97%) with the bacterial flagellar protein gene flgN. A complemented strain flgN+ was constructed and the biological characteristics of the wild-type, mutant A77, and complemented flgN+ strains were investigated. The results showed that the decreased abilities of motility, adhesion to mucus, and biofilm formation in the mutant strain were partially recovered in the complemented flgN+ strain, which suggested that flgN plays an important role in the adhesion of A. hydrophila to its host.
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: X
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Qin
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: G F
authors.initials: GF
authors.lastname: Lin
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: B
authors.initials: B
authors.lastname: Huang
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: W S
authors.initials: WS
authors.lastname: Huang
authors.affiliation: Fisheries College, Jimei University, Xiamen, Fujian, China yanqp@jmu.edu.cn.
authors.forename: Q P
authors.initials: QP
authors.lastname: Yan
mesh_terms: D016980:Aeromonas hydrophila / Q000235:genetics* / Q000472:pathogenicity; D000595:Amino Acid Sequence / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development; D002448:Cell Adhesion / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000235:genetics* / Q000382:microbiology; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D009093:Mucus / Q000378:metabolism / Q000382:microbiology; D016254:Mutagenesis, Insertional; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; C087498:FlgN protein, Bacteria