title: Investigation of multipotent postnatal stem cells from human maxillary sinus membrane.
abstract: Maxillary sinus membrane (MSM) elevation is a common surgical technique for increasing bone height in the posterior maxilla prior to dental implant placement. However, the biological nature of bone regeneration in MSM remains largely unidentified. In this study, MSM tissue was obtained from 16 individuals during orthognathic surgery and used to isolate MSM stem cells (MSMSCs) by single-colony selection and STRO-1 cell sorting. The cell characteristics in terms of colony-forming ability, cell surface antigens, multi-differentiation potential and in vivo implantation were all evaluated. It was found that MSMSCs were of mesenchymal origin and positive for mesenchymal stem cell (MSC) markers such as STRO-1, CD146, CD29 and CD44; furthermore, under defined culture conditions, MSMSCs were able to form mineral deposits and differentiate into adipocytes and chondrocytes. When transplanted into immunocompromised rodents, MSMSCs showed the capacity to generate bone-like tissue and, importantly, maintain their MSC characteristics after in vivo implantation. These findings provide cellular and molecular evidence that MSM contains stem cells that show functional potential in bone regeneration for dental implant.
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China [2] Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: JunBing
authors.initials: J
authors.lastname: Guo
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China [2] Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: JunQuan
authors.initials: J
authors.lastname: Weng
authors.affiliation: Department of Prosthetic Dentistry, the First People's Hospital of Yunnan, Kunming, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Rong
authors.affiliation: Department of Stomatology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China [2] Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: ShuangXi
authors.initials: S
authors.lastname: Zhu
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China [2] Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: DaiYing
authors.initials: D
authors.lastname: Huang
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China [2] Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China [2] Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: SongLing
authors.initials: S
authors.lastname: Chen
mesh_terms: D050156:Adipogenesis; D000293:Adolescent; D053687:Adult Stem Cells / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology; D002469:Cell Separation; D020219:Chondrogenesis; D002999:Clone Cells; D003782:Dental Pulp / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008443:Maxillary Sinus / Q000166:cytology* / Q000000981:diagnostic imaging; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology; D011859:Radiography; D033581:Stem Cell Transplantation; D055815:Young Adult