title: [Relationship of GSTT1 and GSTM1 Gene Polymorphisms and PAH-DNA Adducts with Pathogenesis of Multiple Myeloma].
abstract: OBJECTIVE
  To explore the relationship of GSTT1, GSTM1 gene polymorphisms and PAH-DNA adduct with pathogenesis of multiple myeloma.
  
  
  METHODS
  The bone marrow samples from 37 newly-diagnosed MM patients and 52 healthy peoples as controls were collected; the PCR-based restriction fragment length polymorphism (PCR-RFLP) method was used to detecte the polymorphism of GSTT1 and GSTM1, and to analysis their relationship with clinical characters of MM patients; the engyme linked immunosorbent assay (ELISA) was performed to detect the concentration of PAH-DNA adducts.
  
  
  RESULTS
  GSTT1 null and GSTM1 null genotypes increased the risk of multiple myeloma with OR 2.57 (P=0.035) and 1.37 (P>0.05) respectively. In MM patients group, GSTT1 null genotype in stage III was significantly higher than that in stages I, II (P=0.038). However, no statistically significant association was found between GSTT1 gene polymorphism and clinical characters, such as age, type, hemoglobin, β2-MG, albumin. Compared with Hb≥85 g/L of the newly-diagnosed MM patients, MM patients with Hb<85 g/L had significantly higher incidence of GSTM1 null genetype (P<0.05). The level of PAH-DNA adducts in MM patients was higher than that in controls (2358±1182 pg/ml vs 1853±996 pg/ml) (P<0.05). GSTT1 null genotype with PAH-DNA level≥2100 pg/ml showed a risk index of MM (OR=4.500, P<0.05).
  
  
  CONCLUSIONS
  GSTT1 gene may have a critical function in the development of MM, and correlates with staging of MM. GSTM1 gene polymorphism correlates with hemoglobin levels of patients with MM. The content of PAH-DNA adducts may play an important role in the pathogenesis of multiple myeloma.
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China. E-mail: 18661807392@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Blood Diseases, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Guang-Lun
authors.initials: GL
authors.lastname: Li
authors.affiliation: Central Laboratory, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Li-Kun
authors.initials: LK
authors.lastname: Zhuang
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Lu
mesh_terms: D018736:DNA Adducts; D005838:Genotype; D005982:Glutathione Transferase; D006801:Humans; D015994:Incidence; D009101:Multiple Myeloma; D011084:Polycyclic Aromatic Hydrocarbons; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D018736:DNA Adducts; D005982:Glutathione Transferase; D011084:Polycyclic Aromatic Hydrocarbons; C095911:polycyclic aromatic hydrocarbons-DNA adduct; C413545:glutathione S-transferase T1