title: [Mutation analysis of 16 mutation spots related to children patients with non-syndromic sensorineural hearing loss].
abstract: OBJECTIVE
  To explore the clinical signification of screening 16 target deafness mutations in GJB2, GJB3, SLC26A4, WFS1 and mitochondrial DNA 12S rRNA in 135 children patients with non-syndromic sensorineural hearing loss (NSHL) in Zibo City, Shandong province.
  
  
  METHODS
  Peripheral blood samples of 135 subjects in the study diagnosed as NSHL were collected; Polymerase chain reaction (PCR) and direct sequencing were used to analyze the 16 mutation spots.
  
  
  RESULTS
  Sixty-two cases of 135 patients (45.9%, 62/135) were found out to be carries of at least one pathogenic gene mutation. Among them, 24 cases (17.8%, 24/135) had two mutated alleles (homozygote and compound heterozygote), and 38 cases (28.1%, 38/135) were single mutant carriers. Among all the children patients, 30 cases (22. 2%, 30/135) had SLC26A4 mutations, and 19 cases (14.1%, 19/135) had GJB2 mutations. In the study 86 Mutant alleles were detected, and the allele frequency of SLC26A4 c. 766_2A > G and GJB2 c. 235delC was 11.11% (30/270) and 8.5% (23/270) respectively. The allele frequency of SLC26A4 c. 2168A > G and WFS1 c. 2158A > G is 2.6% (7/270).
  
  
  CONCLUSIONS
  SLC26A4 mutation is the primary cause of the patients with NSHL in this study, and GJB2 mutation is the secondary. The most common mutant form is c. 766_2A of SLC26A4, and the second is c. 235delC of GJB2. GJB3 and WFS1 mutations were detected, whereas mtDNA mutations were not found out in this study.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Man
authors.forename: Zeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Rongzhong
authors.initials: R
authors.lastname: Lu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Shiping
authors.initials: S
authors.lastname: Sun
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Xu
authors.forename: Weiguo
authors.initials: W
authors.lastname: Wang
authors.forename: Huizhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000483:Alleles; D002648:Child; D000072259:Connexin 26; D017630:Connexins; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial; D003638:Deafness; D005787:Gene Frequency; D034381:Hearing Loss; D006319:Hearing Loss, Sensorineural / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008928:Mitochondria; D009154:Mutation; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal
chemical_list: D017630:Connexins; D004272:DNA, Mitochondrial; C000607042:GJB2 protein, human; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D000072259:Connexin 26