title: Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm.
abstract: Three experiments were conducted to determine the protective effect of cholesterol-loaded cyclodextrin (CLC) against hydrogen peroxide (H2O2) or cryo-induced damage in ram sperm. In Experiment 1, the fresh ejaculates were either treated with CLC or remained untreated. Both CLC treated and untreated samples were then incubated with 0, 250 or 500 μM H2O2 at 35°C for 12 h. After incubation period of 12 h, the motility, viability and membrane integrity remained higher in CLC treated sperm even in the presence of 250 or 500 μM H2O2. The H2O2 treatment affected all the sperm parameters adversely (P<0.05). However, compared to CLC untreated counterpart, the motility, viability and membrane integrity remained higher (P<0.05) in treated sperm, even in the presence of 250 or 500 μM H2O2 during 12 h of incubation. In Experiment 2, semen was cryopreserved in the presence or absence of CLC. The post-thaw results revealed that CLC treated sperm has higher (P<0.05) motility, viability and membrane integrity compared to the control. In Experiment 3, lipid peroxidation levels were assessed by determining malondialdehyde (MDA) concentrations during the H2O2-induced oxidative stress in CLC treated and untreated sperm. However, no difference (P>0.05) in MDA level was observed among the groups at any stage of incubation. In conclusion, the CLC incorporation in ram sperm membrane may protects it against H2O2 or cryo-induced oxidative damage. The cryoprotective influence of CLC on ram sperm might be resulted from, at least partly, its antioxidative property.
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey; Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Zahid
authors.initials: Z
authors.lastname: Naseer
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: Ejaz
authors.initials: E
authors.lastname: Ahmad
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey. Electronic address: aksoymelih2003@yahoo.com.
authors.forename: Melih
authors.initials: M
authors.lastname: Aksoy
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: Niyazi
authors.initials: N
authors.lastname: Küçük
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: İlker
authors.initials: İ
authors.lastname: Serin
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ceylan
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Boyacıoğlu
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: Cavit
authors.initials: C
authors.lastname: Kum
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods; D003505:Cyclodextrins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D012661:Semen / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods*; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003505:Cyclodextrins; D008315:Malondialdehyde; D002784:Cholesterol; D006861:Hydrogen Peroxide
keywords: CLC; Cryopreservation; H(2)O(2); Oxidative stress; Ram sperm