title: Online polar two phase countercurrent chromatography×high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step.
abstract: Herein, we report an on-line two-dimensional system constructed by counter-current chromatography (CCC) coupling with preparative high-performance liquid chromatography (prep-HPLC) for the separation and purification of polar natural products. The CCC was used as the first dimensional isolation column, where an environmental friendly polar two-phase solvent system of isopropanol and 16% sodium chloride aqueous solution (1:1.2, v/v) was introduced for low toxicity and favorable resolution. In addition, by applying the stop-and-go flow technique, effluents pre-fractionated by CCC was further purified by a preparative column packed with octadecyl silane (ODS) as the second dimension. The interface between the two dimensions was comprised of a 6-port switching valve and an electronically controlled 2-position 10-port switching valve connected with two equivalent holding columns. To be highlighted here, this rationally designed interface for the purpose of smooth desalination, absorption and desorption, successfully solved the solvent compatibility problem between the two dimensional separation systems. The present integrated system was successfully applied in a one-step preparative separation and identification of 10 pure compounds from the water extracts of Tieguanyin tea (Chinese oolong tea). In short, all the results demonstrated that the on-line 2D CCC×LC method is an efficient and green approach for harvesting polar targets in a single step, which showed great promise in drug discovery.
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Xiamen University, South Si-Ming Road, Xiamen 361005, China.
authors.forename: Shu-Qi
authors.initials: SQ
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Fang
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
authors.forename: Quan-Cheng
authors.initials: QC
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China. Electronic address: wuyl@xmu.edu.cn.
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, South Xiang-An Road, Xiamen 361102, China. Electronic address: qyk@xmu.edu.cn.
authors.forename: Ying-Kun
authors.initials: YK
authors.lastname: Qiu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013662:Tea / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea
keywords: Counter-current chromatography; Polar two-phase solvent system; Tea polyphenols; Tieguanyin tea; Two-dimensional liquid chromatography