title: [Molecular Pathological Diagnosis of Mucosa-associated Lymphoid Tissue Lymphoma].
abstract: OBJECTIVE
  To explore the molecular pathological diagnosis of mucosa-associated lymphoid tissue lymphoma(MALTL).
  
  
  METHODS
  Sixty MALTL paraffin embedding specimens were analyzed retrospectively. The distribution of the primary lesion, morphology, immunophenotype, IgH gene cloning rearrangement were evaluated.
  
  
  RESULTS
  The main risk area for the patients with MALTL was gastric area(37%), in the second place was salivary gland(20%), in the third place was intestine (12%), orbit and ocular adnexa(12%); at low magnification, MALTL specimens manifasted diffuse growth majority, a few of nodular structure, lymphoma cell forms were diversified; The results of immunohistochemical detection showed that the CD20 and the BCL-2 were positive, the CD3, CD5, CD10, CD23, cyclin D1 and CD21 were negative, 17 specimen kappa or lambda express more obviously, their sensibility was 28.33%(17/60); 61.67%(37/60) developed IgH gene rearrangement, 19 specimen IgH gene rearrangement monoclonal and kappa or lambda were negative, the positive rate of both combined detections was 68.33%.
  
  
  CONCLUSIONS
  The tissue morphologic characteristics and immuno-histochemistry detection are the basic means for MALTL diagnosis, the detection of IgH gene reasragement and Kappa or lamda restrictive expression has the practical importance for MALTL diagnosis, both combination can show higher positive rate for MALTL diagnosis.
authors.affiliation: Department of Pathology, Shengzhou Minicipal People Hospital, Shengzhou 312400, Zhejiang Province, China. E-mail: jianfengzhangsz@163.com.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Shengzhou Minicipal People Hospital, Shengzhou 312400, Zhejiang Province, China.
authors.forename: Shu-Xia
authors.initials: SX
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D019938:Cyclin D1; D015321:Gene Rearrangement; D006801:Humans; D016130:Immunophenotyping; D018442:Lymphoma, B-Cell, Marginal Zone
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1