title: Adsorption of nucleotides on biomimetic apatite: The case of cytidine 5' monophosphate (CMP).
abstract: The chemical interaction between DNA macromolecules and hard tissues in vertebrate is of foremost importance in paleogenetics, as bones and teeth represent a major substrate for the genetic material after cell death. Recently, the empirical hypothesis of DNA "protection" over time thanks to its adsorption on hard tissues was revisited from a physico-chemical viewpoint. In particular, the existence of a strong interaction between phosphate groups of DNA backbone and the surface of apatite nanocrystals (mimicking bone/dentin mineral) was evidenced on an experimental basis. In the field of nanomedicine, DNA or RNA can be used for gene transport into cells, and apatite nanocarriers then appear promising. In order to shed some more light on interactions between DNA molecules and apatite, the present study focuses on the adsorption of a "model" nucleotide, cytidine 5' monophosphate (CMP), on a carbonated biomimetic apatite sample. The follow-up of CMP kinetics of adsorption pointed out the rapidity of interaction with stabilization reached within few minutes. The adsorption isotherm could be realistically fitted to the Sips model (Langmuir-Freundlich) suggesting the influence of surface heterogeneities and adsorption cooperativity in the adsorption process. The desorption study pointed out the reversible character of CMP adsorption on biomimetic apatite. This contribution is intended to prove helpful in view of better apprehending the molecular interaction of DNA fragments and apatite compounds, independently of the application domain, such as bone diagenesis or nanomedicine. This study may also appear informative for researchers interested in the origins of life on Earth and the occurrence and behavior of primitive biomolecules.
authors.affiliation: CIRIMAT Carnot Institute, UMR CNRS/INPT/UPS 5085, University of Toulouse, Ensiacet, 4 allée E. Monso, 31030 Toulouse cedex 4, France; CIRIMAT Carnot Institute, UMR CNRS/INPT/UPS 5085, University of Toulouse, Faculté des Sciences Pharmaceutiques, 35 Chemin des Maraîchers, 31400 Toulouse, France.
authors.forename: Maëla
authors.initials: M
authors.lastname: Choimet
authors.affiliation: CIRIMAT Carnot Institute, UMR CNRS/INPT/UPS 5085, University of Toulouse, Faculté des Sciences Pharmaceutiques, 35 Chemin des Maraîchers, 31400 Toulouse, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Tourrette
authors.affiliation: CIRIMAT Carnot Institute, UMR CNRS/INPT/UPS 5085, University of Toulouse, Ensiacet, 4 allée E. Monso, 31030 Toulouse cedex 4, France. Electronic address: christophe.drouet@ensiacet.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Drouet
mesh_terms: D000327:Adsorption; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry; D032701:Biomimetics; D001842:Bone and Bones / Q000737:chemistry; D002113:Calcification, Physiologic; D003568:Cytidine Monophosphate / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D003804:Dentin; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D053758:Nanoparticles / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D010710:Phosphates; D012313:RNA / Q000737:chemistry; D051381:Rats; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D009711:Nucleotides; D010710:Phosphates; D012313:RNA; D004247:DNA; D017886:Durapatite; D003568:Cytidine Monophosphate
keywords: Adsorption; Biomimetic apatite; Cytidine monophosphate; DNA; Hydroxyapatite; Paleogenetics