title: Myosin regulatory light chain phosphorylation enhances cardiac β-myosin in vitro motility under load.
abstract: Familial hypertrophic cardiomyopathy (HCM) is characterized by left ventricular hypertrophy and myofibrillar disarray, and often results in sudden cardiac death. Two HCM mutations, N47K and R58Q, are located in the myosin regulatory light chain (RLC). The RLC mechanically stabilizes the myosin lever arm, which is crucial to myosin's ability to transmit contractile force. The N47K and R58Q mutations have previously been shown to reduce actin filament velocity under load, stemming from a more compliant lever arm (Greenberg, 2010). In contrast, RLC phosphorylation was shown to impart stiffness to the myosin lever arm (Greenberg, 2009). We hypothesized that phosphorylation of the mutant HCM-RLC may mitigate distinct mutation-induced structural and functional abnormalities. In vitro motility assays were utilized to investigate the effects of RLC phosphorylation on the HCM-RLC mutant phenotype in the presence of an α-actinin frictional load. Porcine cardiac β-myosin was depleted of its native RLC and reconstituted with mutant or wild-type human RLC in phosphorylated or non-phosphorylated form. Consistent with previous findings, in the presence of load, myosin bearing the HCM mutations reduced actin sliding velocity compared to WT resulting in 31-41% reductions in force production. Myosin containing phosphorylated RLC (WT or mutant) increased sliding velocity and also restored mutant myosin force production to near WT unphosphorylated values. These results point to RLC phosphorylation as a general mechanism to increase force production of the individual myosin motor and as a potential target to ameliorate the HCM-induced phenotype at the molecular level.
authors.affiliation: Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Karabina
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kazmierczak
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Danuta
authors.initials: D
authors.lastname: Szczesna-Cordary
authors.affiliation: Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA, USA. Electronic address: Jeffrey_Moore@uml.edu.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Moore
mesh_terms: D000185:Actinin / Q000737:chemistry* / Q000235:genetics; D000199:Actins / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D002645:Chickens; D015870:Gene Expression; D006352:Heart Ventricles / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D009038:Motion; D018482:Muscle, Skeletal / Q000737:chemistry; D009130:Muscle, Smooth / Q000737:chemistry; D009154:Mutation; D018994:Myosin Light Chains / Q000737:chemistry* / Q000235:genetics; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry* / Q000235:genetics; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013552:Swine; D024723:Ventricular Myosins / Q000737:chemistry* / Q000235:genetics
chemical_list: D000199:Actins; D018994:Myosin Light Chains; D011994:Recombinant Proteins; D000185:Actinin; D009219:Myosin-Light-Chain Kinase; D024723:Ventricular Myosins
keywords: Cardiac ventricular myosin; Hypertrophic cardiomyopathy; Load dependence; Regulatory light chain phosphorylation