title: [Inhibitory effect of 17-AAG combined with paclitaxel on proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of 17-AAG combined with paclitaxel (PTX) on the proliferation and apoptosis of esophageal squamous cell carcinoma cell line Eca-109 in vitro.
  
  
  METHODS
  Eca-109 cells were treated with 17-AAG and PTX either alone or in combination. The proliferation of Eca-109 cells was detected by MTT assay, and the cell cycle changes and cell apoptosis were determined by flow cytometry.
  
  
  RESULTS
  Compared with the control group, both 17-AAG and PTX significantly inhibited the proliferation of Eca-109 cells. A combined treatment of the cells with 0.5 µmol/L PTX and 0.625 µmol/L 17-AAG produced an obviously stronger inhibitory effect on the cell proliferation than either of the agents used alone (P<0.01). Flow cytometry showed that, 17-AAG and PTX used alone caused Eca-109 cell cycle arrest in G2/M phase and S phase, respectively, and their combined use caused cell cycle arrest in both G2/M and S phases. The cell apoptosis rates of Eca-109 cells treated with 17-AAG, PTX and their combination were 4.52%, 10.91%, and 29.88%, respectively, all significantly higher than that in the control group (1.32%); the combined treatment resulted in a distinct apoptotic peak that was significantly higher than that caused by either of the agents alone.
  
  
  CONCLUSIONS
  17-AAG and PTX can inhibit cell proliferation and promote apoptosis of Eca-109 cells, and their combination produces stronger effects in inhibiting cell proliferation and increasing cell apoptosis.
authors.affiliation: Department of Oncology, First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China. E-mail: 13710956393@139.com.
authors.forename: Size
authors.initials: S
authors.lastname: Chen
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Li
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.forename: Xianyi
authors.initials: X
authors.lastname: Mo
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Kailan
authors.initials: K
authors.lastname: Mo
authors.forename: Ying
authors.initials: Y
authors.lastname: Ding
mesh_terms: D017209:Apoptosis; D016227:Benzoquinones / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D047029:Lactams, Macrocyclic / Q000494:pharmacology*; D017239:Paclitaxel / Q000494:pharmacology*
chemical_list: D016227:Benzoquinones; D047029:Lactams, Macrocyclic; C112765:tanespimycin; D017239:Paclitaxel