title: Rapid implantation of dissolving microneedles on an electrospun pillar array.
abstract: Dissolving microneedles (DMNs), designed to release drugs and dissolve after skin insertion, have been spotlighted as a novel transdermal delivery system due to their advantages such as minimal pain and tissue damage, ability to self-administer, and no associated hazardous residues. The drug delivery efficacy of DMNs, however, is limited by incomplete insertion and the extended period required for DMN dissolution. Here, we introduce a novel DMN delivery system, DMN on an electrospun pillar array (DEPA), which can rapidly implant DMNs into skin. DMNs were fabricated on a pillar array covered by a fibrous sheet produced by electrospinning PLGA solution (14%, w/v). DMNs were implanted into the skin by manual application (press and vibration for 10 s) by tearing of the fibers hung on the 300-μm pillars. Separation of DMNs from the fibrous sheet was dependent on both pillar height and the properties of the fibrous sheet. After evaluation of the implantation and dissolution of DMNs with diffusion of red dye by taking cross-sectional images of porcine skin, the hypoglycemic effect of insulin loaded DEPA was examined using a healthy mouse model. This DMN array overcomes critical issues associated with the low penetration efficiency of flat patch-based DMNs, and will allow realization of patient convenience with the desired drug efficacy.
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors.forename: Huisuk
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors.forename: Soyoung
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors.forename: Inyoung
authors.initials: I
authors.lastname: Huh
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors.forename: Suyong
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors.forename: Shayan F
authors.initials: SF
authors.lastname: Lahiji
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea.
authors.forename: Miroo
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seoul 120-749, Republic of Korea. Electronic address: hijung@yonsei.ac.kr.
authors.forename: Hyungil
authors.initials: H
authors.lastname: Jung
mesh_terms: D020341:Absorbable Implants; D000279:Administration, Cutaneous; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D004339:Drug Compounding; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004343:Drug Implants / Q000008:administration & dosage* / Q000627:therapeutic use; D004867:Equipment Design; D057927:Hydrophobic and Hydrophilic Interactions; D007328:Insulin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D019344:Lactic Acid; D008297:Male; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012867:Skin / Q000648:ultrastructure; D050151:Subcutaneous Fat; D034421:Sus scrofa; D013552:Swine; D013718:Tensile Strength
chemical_list: D001786:Blood Glucose; D004343:Drug Implants; D007328:Insulin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Dissolving microneedles on electrospun pillar array; Droplet-born air blowing; Electrospinning; Implantation