title: Genome-wide analysis reveals phytohormone action during cassava storage root initiation.
abstract: Development of storage roots is a process associated with a phase change from cell division and elongation to radial growth and accumulation of massive amounts of reserve substances such as starch. Knowledge of the regulation of cassava storage root formation has accumulated over time; however, gene regulation during the initiation and early stage of storage root development is still poorly understood. In this study, transcription profiling of fibrous, intermediate and storage roots at eight weeks old were investigated using a 60-mer-oligo microarray. Transcription and gene expression were found to be the key regulating processes during the transition stage from fibrous to intermediate roots, while homeostasis and signal transduction influenced regulation from intermediate roots to storage roots. Clustering analysis of significant genes and transcription factors (TF) indicated that a number of phytohormone-related TF were differentially expressed; therefore, phytohormone-related genes were assembled into a network of correlative nodes. We propose a model showing the relationship between KNOX1 and phytohormones during storage root initiation. Exogeneous treatment of phytohormones N (6) -benzylaminopurine and 1-Naphthaleneacetic acid were used to induce the storage root initiation stage and to investigate expression patterns of the genes involved in storage root initiation. The results support the hypothesis that phytohormones are acting in concert to regulate the onset of cassava storage root development. Moreover, MeAGL20 is a factor that might play an important role at the onset of storage root initiation when the root tip becomes swollen.
authors.affiliation: Department of Biotechnology, Center for Cassava Molecular Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand, punchapat.soj@mahidol.ac.th.
authors.forename: Punchapat
authors.initials: P
authors.lastname: Sojikul
authors.forename: Treenut
authors.initials: T
authors.lastname: Saithong
authors.forename: Saowalak
authors.initials: S
authors.lastname: Kalapanulak
authors.forename: Nuttapat
authors.initials: N
authors.lastname: Pisuttinusart
authors.forename: Siripan
authors.initials: S
authors.lastname: Limsirichaikul
authors.forename: Maho
authors.initials: M
authors.lastname: Tanaka
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Utsumi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Jarunya
authors.initials: J
authors.lastname: Narangajavana
mesh_terms: D003583:Cytokinins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018745:Genome, Plant / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D002365:Manihot / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators