title: Urokinase receptor-deficient mice mount an innate immune response to and clarify respiratory viruses as efficiently as wild-type mice.
abstract: The plasminogen activator receptor (uPAR) is required for lung infiltration by innate immune cells in respiratory bacterial infections. In order to verify if this held true for respiratory viruses, wild type (WT) and uPAR knockout (uPAR(-/-)) mice were inoculated intranasally with the human respiratory syncytial virus (HRSV) and the influenza A virus. At several days post-infection (dpi), viral titers in the lungs were determined while cell infiltrates in the bronchoalveolar lavage (BAL) were analyzed by flow cytometry. In the case of influenza A, body weight loss and mortality were also monitored. Only minor differences were observed between infected WT and uPAR(-/-) mice, primarily in influenza virus replication and pathology. These results indicate that uPAR does not play a major role in limiting virus replication or in orchestrating the innate immune response against HRSV or influenza infections in mice. This suggests that there are fundamental differences in the immune control of the viral infections studied here and those caused by bacteria.
authors.affiliation: a Unidad de Inmunología Viral; Centro Nacional de Microbiología; Instituto de Salud Carlos III ; Madrid , Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Ramos
authors.affiliation: a Unidad de Inmunología Viral; Centro Nacional de Microbiología; Instituto de Salud Carlos III ; Madrid , Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Lao
authors.affiliation: b Unidad de Veterinaria; Instituto de Salud Carlos III ; Madrid , Spain.
authors.forename: César
authors.initials: C
authors.lastname: Eguiluz
authors.affiliation: c Centro de Biología Molecular Severo Ochoa; CSIC/Universidad Autónoma de Madrid ; Madrid , Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Del Val
authors.affiliation: d Unidad de Infección Viral e Inmunidad; Centro Nacional de Microbiología; Instituto de Salud Carlos III ; Madrid , Spain.|e Centro de Investigación Biomédica en Red. Enfermedades Respiratorias; Instituto de Salud Carlos III ; Madrid , Spain.
authors.forename: Isidoro
authors.initials: I
authors.lastname: Martínez
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology / Q000472:pathogenicity; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055293:Receptors, Urokinase Plasminogen Activator / Q000172:deficiency* / Q000276:immunology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology / Q000472:pathogenicity; D012141:Respiratory Tract Infections / Q000276:immunology* / Q000821:virology*; D016019:Survival Analysis; D014568:Urokinase-Type Plasminogen Activator / Q000172:deficiency* / Q000276:immunology; D019562:Viral Load; D014779:Virus Replication / Q000502:physiology
chemical_list: D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator
keywords: influenza A virus; innate immune response; respiratory syncytial virus; urokinase plasminogen activator receptor (uPAR)