title: Goldfish Carassius auratus susceptibility to viral hemorrhagic septicemia virus genotype IVb depends on exposure route.
abstract: We assessed the susceptibility of goldfish Carassius auratus to infection by genotype IVb of the viral hemorrhagic septicemia virus. Goldfish were infected by intraperitoneal injections of 106 plaque-forming units (pfu) fish-1, single bath exposure of 105 pfu ml-1 for 24 h, or consumption of 0.4 g of commercial fish feed soaked in 107 pfu per 8 fish. The mortality rate of intraperitoneal-infected goldfish was 10 to 32%, although the virus was detected by quantitative RT-PCR in 77% (65/84) of the survivors at the end of the 42 d trial, suggesting a carrier state. Severe gross lesions were observed in many of the moribund and dead goldfish such as hemorrhaging in the skin, fin, liver, kidney, brain, intestine, and eye as well as abdominal distension, bilateral exophthalmia, and splenomegaly. There was minimal morbidity or mortality in the immersion, feeding, or control groups.
authors.affiliation: Aquatic Animal Health Program, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY 14853, USA.
authors.forename: Rodman G
authors.initials: RG
authors.lastname: Getchell
authors.forename: Toni
authors.initials: T
authors.lastname: Erkinharju
authors.forename: Anna O
authors.initials: AO
authors.lastname: Johnson
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Davis
authors.forename: Emily E
authors.initials: EE
authors.lastname: Hatch
authors.forename: Emily R
authors.initials: ER
authors.lastname: Cornwell
authors.forename: Paul R
authors.initials: PR
authors.lastname: Bowser
mesh_terms: D000818:Animals; D004198:Disease Susceptibility; D005393:Fish Diseases / Q000821:virology*; D005838:Genotype; D006054:Goldfish; D029141:Novirhabdovirus / Q000235:genetics*; D018353:Rhabdoviridae Infections / Q000662:veterinary* / Q000821:virology