title: Reference gene selection for real-time quantitative PCR analysis on ovarian cryopreservation by vitrification in mice.
abstract: OBJECTIVE
  To ensure the correct interpretation of the results of quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) from ovarian tissue cryopreserved by vitrification, it is critical to normalize expression levels to a reference gene with stable messenger RNA (mRNA) expression in the vitrified/warmed ovarian tissue. The aim of this work was to identify suitable reference genes for qRT-PCR analysis during ovarian cryopreservation by vitrification.
  
  
  METHODS
  GeNorm, NormFinder, comparative Delta-CT, and BestKeeper were used to analyze the expression and stability of the 14 reference genes GAPDH, ABL1, ACTB, CDKN1A, GPER, GUSB, HPRT1, HSP90AB1, IPO8, PPIA, RPL4, RPL30, TBP, and UPAR.
  
  
  RESULTS
  Our results indicated that ACTB and RPL4 were relatively stable reference genes in vitrified/warmed ovaries.
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetics in Ningxia, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan, Ningxia, 75004, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yuanyuan
authors.forename: Su
authors.initials: S
authors.lastname: Qin
authors.forename: Xu
authors.initials: X
authors.lastname: Rongrong
authors.forename: Gao
authors.initials: G
authors.lastname: Yujing
authors.forename: Pei
authors.initials: P
authors.lastname: Chengbin
authors.forename: Ma
authors.initials: M
authors.lastname: Jianjun
authors.forename: Yang
authors.initials: Y
authors.lastname: Yanzhou
authors.forename: Pei
authors.initials: P
authors.lastname: Xiuying
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D008810:Mice, Inbred C57BL; D010053:Ovary / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D012269:Ribosomal Proteins / Q000235:genetics; D058989:Vitrification
chemical_list: D000199:Actins; D012269:Ribosomal Proteins; C056368:ribosomal protein L4; C000608517:ribosomal protein L4, mouse