title: Modeling and simulation of fructooligosaccharides synthesis in a batch basket reactor.
abstract: Fructooligosaccharides (FOS) production was carried out in a batch basket reactor with immobilized fructosyltransferase from Rhodotorula sp. from 500×10(3) g m(-3) of sucrose in 50 mM sodium acetate buffer at pH 6.0, 48 °C at 85 rpm and with an activity of 22.44×10(3) U m(-3). The experimental data were well adjusted to the mathematical model for FOS production using SIMULINK(®) (MATLAB(®)). The highest regression coefficient (R(2)>90%) and the lowest percentual residual standard deviation (%RSD<4.0) and chi-square (χ(2) <1.0) were obtained for sucrose (GF), kestose (GF2) and total FOS. The mass transfer coefficient (kL) was determined as 5.6×10(-5) m h(-1) and the diffusivity (DS) was 2.11×10(-11) m(2) s(-1). The best predicted FOS yield (after 96 h) was 60.62%, with an equivalent productivity of 3.16×10(3) g m(-3) h(-1). These results reaffirm the good potential of this enzyme for industrial application and, in addition, are in conformation to other studies conducted with the same enzyme from the same and different microbial sources.
authors.affiliation: Department of Exact and Land Sciences, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil.
authors.forename: Maiki Roberto
authors.initials: MR
authors.lastname: Detofol
authors.affiliation: Department of Bioscience, Multidisciplinary Institute on Health (IMS), Federal University of Bahia campus Anísio Teixeira (CAT/UFBA), R. Rio de Contas, 80, Candeias, Postal Code: 45.029-094, Vitória da Conquista, Bahia, Brazil. Electronic address: elizamaguiar@yahoo.com.br.
authors.forename: Elizama
authors.initials: E
authors.lastname: Aguiar-Oliveira
authors.affiliation: Department of Engineering and Computer Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil.
authors.forename: Cindy Elena
authors.initials: CE
authors.lastname: Bustamante-Vargas
authors.affiliation: Department of Agricultural Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99.700-000, Erechim, Rio Grande do Sul, Brazil.
authors.forename: Alexandre Batista de Jesus
authors.initials: AB
authors.lastname: Soares
authors.affiliation: Department of Engineering and Computer Science, Integrated Regional University of the Alto Uruguai and Missões (URI), Av Sete de Setembro 1621, Postal Code: 99700-000, Erechim, Rio Grande do Sul, Brazil.
authors.forename: Mónica Beatriz Alvarado
authors.initials: MB
authors.lastname: Soares
authors.affiliation: Department of Food Engineer, Faculty of Food Engineer (FEA), University of Campinas (UNICAMP), R. Monteiro Lobato, 80, Cidade Universitária, Postal Code: 13083-862. Campinas, São Paulo, Brazil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Maugeri
mesh_terms: D019149:Bioreactors; D003198:Computer Simulation; D004800:Enzymes, Immobilized / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D008962:Models, Theoretical; D009844:Oligosaccharides / Q000096:biosynthesis*; D012248:Rhodotorula / Q000201:enzymology*; D001426:Bacterial Proteins
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D006602:Hexosyltransferases; D009844:Oligosaccharides; C116580:fructooligosaccharide; C021451:inulosucrase; D001426:Bacterial Proteins
keywords: Basket Reactor; FOS Production; Fructosyltransferase; Kinetic; Rhodotorula