title: miR-30c negatively regulates the migration and invasion by targeting the immediate early response protein 2 in SMMC-7721 and HepG2 cells.
abstract: miR-30c has been reported to act as a tumor suppressor and negatively regulate cancer metastasis by directly targeting metastasis associated genes; however, miR-30c has also been shown to promote the invasion of metastatic breast cancer cells, suggesting that miR-30c might be involved in cancer cell metastasis in different ways via targeting different genes. In this study, we demonstrated that over-expression and knockdown of immediate early response protein 2 (IER2) modulated the general capacity of the migration and invasion in hepatocellular carcinoma cell line SMMC-7721 and HepG2, whereas overexpression and knockdown of miR-30c decreased and promoted cell motility, respectively. Further studies revealed that miR-30c overexpression down-regulated the expression of IER2 protein but not its mRNA level, and miR-30c can directly target the 3' untranslated region (3'UTR) of IER2, and subsequently reducing its expression. Moreover, we also showed that suppression of cell motility by miR-30c was partially rescued by IER2 re-expression. Our results indicated that miR-30c may function as a negative regulator in cell motility, with IER2 as a direct and functional target in SMMC-7721 and HepG2 cells.
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China ; Clinical Medical College, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.affiliation: Clinical Medical College, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Xizhi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Liao
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Zijing
authors.initials: Z
authors.lastname: Deng
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Shen
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Medicine, Yangzhou University Yangzhou, Jiangsu 225001, P.R. China ; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou, Jiangsu 225001, P.R. China ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou, Jiangsu 225009, P.R. China.
authors.forename: Weigan
authors.initials: W
authors.lastname: Shen
keywords: IER2; invasion; miR-30c; migration