title: Rice stripe1-2 and stripe1-3 Mutants Encoding the Small Subunit of Ribonucleotide Reductase Are Temperature Sensitive and Are Required for Chlorophyll Biosynthesis.
abstract: We induced mutants, stripe1-2 (st1-2) and stripe1-3 (st1-3), from rice (Oryza sativa L.) Indica 9311 using Ethyl methanesulfonate (EMS). Both st1-2 and st1-3 mutants encoded the small subunit of ribonucleotide reductase 1 (RNRS1), differed in the location of the mutated base, and displayed white-stripe from the L2 stage through maturity. The mutants were sensitive to temperature, and their chlorophyll content increased with the increase in temperature; however, they did not revert to normal green leaf phenotype under field conditions. The mutant st1-2 showed loosely arranged thylakoid lamellar structure as compared with wild-type (WT) plants. Contrastingly, st1-3 displayed normal thylakoid lamellar structure, good agronomic traits, and higher yield than st1-2 but lower yield than WT. Three-dimensional structure prediction for RNRS1 indicated that the mutation in Val-171 residue in st1-2 influenced the connection of RNRS1 to iron, causing abnormal development of chloroplasts. Real-time PCR analysis showed that the expression levels associated with chlorophyll biosynthetic pathway and photosynthesis were affected in st1-2 and st1-3 at different temperatures and different developmental stages.
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Long
authors.initials: L
authors.lastname: Xin
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Kangxi
authors.initials: K
authors.lastname: Du
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Ran
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Cui
authors.affiliation: College of Resources, Sichuan Agricultural University, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Quanju
authors.initials: Q
authors.lastname: Xiang
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Peizhou
authors.initials: P
authors.lastname: Xu
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Wu
mesh_terms: D002734:Chlorophyll / Q000096:biosynthesis*; D017343:Genes, Plant; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D012264:Ribonucleotide Reductases / Q000235:genetics*; D013696:Temperature
chemical_list: D002734:Chlorophyll; D012264:Ribonucleotide Reductases