title: Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai.
abstract: A new broad-spectrum powdery mildew resistance allele Pm2c was identified and mapped in Chinese wheat landrace Niaomai. Chinese wheat landrace Niaomai showed resistance to 27 of 28 Chinese Blumeria graminis f. sp tritici (Bgt) races. Genetic analysis of an F2 population and its derived F2:3 families from the cross Niaomai × Mingxian 169 and backcross population, Niaomai/2*Mingxian 169, indicated that the resistance of Niaomai to Bgt races was conferred by a single dominant resistance gene, temporarily designated PmNM. Molecular tagging showed that PmNM was located on chromosome 5DS and flanked by SSR markers Xcfd81 and Xcfd78 with the genetic distances of 0.1/0.4 cM and 4.9/7.5 cM, respectively. Niaomai showed a different array of responses compared to lines with Pm2a, Pm2b, PmD57-5D, PmLX66, PmX3986-2 and Pm48 genes, sharing the same Xcfd81 allele but differing from Xcfd78 allele for Pm2a and Pm2b lines. Allelism tests based on crosses of Niaomai with Ulka/8*Cc and KM2939 showed that PmNM is allelic to Pm2a and Pm2b. We concluded that PmNM is a new allele of Pm2, re-designated Pm2c. Pm2c could be transferred into wheat cultivars by marker-assisted selection to improve the powdery mildew resistance of breeding cultivars/lines.
authors.affiliation: Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Bioengineering, Henan University of Technology, Zhengzhou, 450001, Henan, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Ma
authors.affiliation: Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Qie
authors.affiliation: Shijiazhuang Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050041, Hebei, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Fu
authors.affiliation: Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China. dgan@sjziam.ac.cn.
authors.forename: Diaoguo
authors.initials: D
authors.lastname: An
mesh_terms: D000483:Alleles; D001203:Ascomycota; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D005819:Genetic Markers