title: Urinary Metabolomic Profiling Reveals the Effect of Shenfu Decoction on Chronic Heart Failure in Rats.
abstract: Shenfu decoction (SFD) can be used to treat patients with sign of Yangqi decline or Yang exhaustion related to chronic heart failure (CHF). We conducted a gas chromatography with time-of-flight mass spectrometer (GC/TOF-MS)-based metabolomic study to increase the understanding of CHF and assess the efficacies and mechanisms of SFD in treating CHF induced by coronary artery ligation in rats. Based on unsupervised principal component analysis, there was a clear separation between the CHF and sham surgery group, which revealed that CHF disturbed the metabolism of endogenous substances and significantly altered the urine metabolite fingerprints. After SFD treatment, the metabolomics profile found in CHF was significantly reversed, shifting much closer to normal controls and sham surgery group, indicating that SFD has therapeutic effects in CHF, which is in accordance with the hemodynamic assay results. Metabolomic pathway analysis demonstrated that several pathways including fatty acid biosynthesis, fatty acid elongation, steroid biosynthesis, galactose metabolism, and amino acid metabolism were significantly altered in CHF rats. Therefore, we may infer that SFD shows therapeutic efficacy in CHF by restoring these disturbed metabolic pathways, especially those related to energy metabolism. This study offers new methodologies for increasing the understanding of CHF and systematically characterizing the efficacies and mechanisms of SFD in treating CHF.
authors.affiliation: Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, 67 Dong Chang Xi Lu, Liaocheng 252000, China. daweiyang2011@gmail.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China. xxshining@163.com.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, 67 Dong Chang Xi Lu, Liaocheng 252000, China. u_m_c_u_ywu@hotmail.com.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China. goldlubo@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.affiliation: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China. yuanaifeng07@sina.com.
authors.forename: Aifeng
authors.initials: A
authors.lastname: Yuan
authors.affiliation: Biomedical Engineering School, Carlos III University, Avda Universidad 30, Leganes, Madrid 28911, Spain. cleon@ucdavis.edu.|Metabolomics, Genome Center, UC Davis, 451 Health Sciences Drive, Davis, CA 95616, USA. cleon@ucdavis.edu.
authors.forename: Carlos
authors.initials: C
authors.lastname: Leon
authors.affiliation: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China. guona5246@126.com.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008401:Gas Chromatography-Mass Spectrometry; D006333:Heart Failure / Q000188:drug therapy* / Q000503:physiopathology; D006439:Hemodynamics; D008297:Male; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D016482:Urinalysis
chemical_list: D004365:Drugs, Chinese Herbal; C000590839:Shenghua Decoction
keywords: GC/TOF–MS; Shenfu decoction; chronic heart failure; metabolomic pathway analysis; metabolomics