title: Effect of alcoholic extract of Entada pursaetha DC on monosodium iodoacetate-induced osteoarthritis pain in rats.
abstract: OBJECTIVE
  Osteoarthritis (OA) is a degenerative disease characterized by joint pain and progressive loss of articular cartilage. Entada pursaetha has been traditionally used in the treatment of inflammatory disease, liver ailment, etc. In this study we investigated suppressive effect of ethanolic extract of E. pursaetha (EPE) on monosodium iodoacetate (MIA)-induced osteoarthritis pain and disease progression by histopathological changes in joints in a rat model.
  
  
  METHODS
  OA was induced in right knee of rat by intra-articular injection of 3 mg of MIA and characterized by pathological progression of disease and pain of affected joint. Spontaneous movements, mechanical, thermal and cold sensitivity were monitored at days 0 (before drug and MIA injection), 7, 14 and 21 of MIA administration. EPE (30, 100 and 300 mg/kg), vehicle or etoricoxib (10 mg/ kg; reference drug) were administered daily for 21 days by oral route.
  
  
  RESULTS
  EPE at various doses significantly reduced mechanical, heat, cold hyperalgesia and increased the horizontal and vertical movements in intra-articular MIA injected rats. EPE prevented the damage to cartilage structure and reduced the cellular abnormalities. Articular cartilage of rats treated with EPE at 300 mg/kg group was almost normal with well-developed smooth surface and chondrocytes were distributed individually or arranged in column.
  
  
  CONCLUSIONS
  The present findings showed that the EPE was not only able to mitigate pain and hyperalgesia but also inhibited MIA-induced cartilage degeneration in vivo. EPE may have the potential to become therapeutic modality in the treatment of osteoarthritis. However, further studies need to be done to confirm these findings in other models and clinical trials.
authors.forename: Rashmi R
authors.initials: RR
authors.lastname: Kumari
authors.forename: Amar S
authors.initials: AS
authors.lastname: More
authors.forename: Gaurav
authors.initials: G
authors.lastname: Gupta
authors.forename: Madhu C
authors.initials: MC
authors.lastname: Lingaraju
authors.forename: Venkanna
authors.initials: V
authors.lastname: Balaganur
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kumar
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Division of Pharmacology & Toxicology, Indian Veterinary Research Institute, Izatnagar, India.
authors.forename: Surendra Kumar
authors.initials: SK
authors.lastname: Tandan
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D002358:Cartilage, Articular / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects; D004195:Disease Models, Animal; D007887:Fabaceae / Q000737:chemistry; D006801:Humans; D007270:Injections, Intra-Articular; D007461:Iodoacetates / Q000633:toxicity; D008297:Male; D010003:Osteoarthritis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D010146:Pain / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats
chemical_list: D007461:Iodoacetates; D010936:Plant Extracts