title: Antioxidant and Antiacetylcholinesterase Activity of Teucrium hyrcanicum.
abstract: BACKGROUND
  Teucrium hyrcanicum belonging to the Lamiaceae family is a native plant in Iran; it is called Maryam nokhodi-e-jangali in Farsi.
  
  
  OBJECTIVE
  The aim of this study is to evaluate acetylcholinesterase inhibition (AChEI), antioxidant activity and flavonoids content of T. hyrcanicum methanol extract.
  
  
  METHODS
  The air-dried and the ground aerial parts of T. hyrcanicum were extracted by percolation method with methanol. Antioxidant activity of the extract was investigated by using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay (FRAP) methods. In addition, AChEI and flavonoid content of T. hyrcanicum methanol extract were measured.
  
  
  RESULTS
  The results showed that total flavonoid content of T. hyrcanicum in reference to the standard curve for quercetin was 20.70 ± 0.05 mg quercetin equivalents/g of extract. In the FRAP method, the antioxidant activity of T. hyrcanicum extract and butyl hydroxyanisole (BHA) (as a positive control) were 657.5 ± 0.04 and 880 ± 0.06 mmol Fe II/1 g dried extract. According to results of DPPH assay, half maximal inhibitory concentration (IC50) value for DPPH radical-scavenging activities of T. hyrcanicum methanol extract, vitamin E and BHA were 74.6, 14.12 and 7.8 μg/mL, respectively. IC50 value for AChEI of T. hyrcanicum and donepezil as a positive control were 2.12 mg/mL and 0.013 mg/mL.
  
  
  CONCLUSIONS
  The results of the present study showed T. hyrcanicum is a natural antioxidant that the flavonoid content can be responsible for extract effects.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Golfakhrabadi
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran ; Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Yousefbeyk
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran.
authors.forename: Tahmineh
authors.initials: T
authors.lastname: Mirnezami
authors.affiliation: Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Pedram
authors.initials: P
authors.lastname: Laghaei
authors.affiliation: Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran ; Department of Traditional Pharmacy, Faculty of Traditional Medicine and Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran.
authors.forename: Mannan
authors.initials: M
authors.lastname: Hajimahmoodi
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran ; Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Khanavi
keywords: Acetylcholinesterase inhibitory; Teucrium hyrcanicum; antioxidant activity; flavonoid contents