title: Olfr603, an orphan olfactory receptor, is expressed in multiple specific embryonic tissues.
abstract: BACKGROUND
  Olfactory receptors were initially believed to be expressed specifically within the olfactory neurons. However, accumulating genome-scale data has recently demonstrated more extensive expression. There are hundreds of olfactory receptor family members and the realisation of their widespread expression provides an opportunity to reveal new biology. However, existing data is predominantly based on RT-PCR, microarray and RNA-seq approaches and the details of tissue and cell-type specific expression are lacking.
  
  
  RESULTS
  As a proof of principle, we selected Olfr603 for expression analysis. We generated an antibody against a non-conserved epitope of Olfr603 and characterised its expression in E8.5-E12.5 mouse embryos using immunohistochemistry. This analysis demonstrated a dynamic pattern of expression in diverse cell types within the developing embryo unrelated to the olfactory system. Expression was detected in migrating neural crest, endothelial precursors and vascular endothelium, endocardial cells, smooth muscle, neuroepithelium and within the ocular tissues. This complex distribution does not conform to any apparent germ layer or tissue origin.
  
  
  CONCLUSIONS
  This initial characterisation of Olfr603 expression highlights the potential for a broad role for this receptor in the development of many tissues.
authors.affiliation: Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville and Department of Paediatrics, University of Melbourne, Parkville, Australia.
authors.forename: Naomi L
authors.initials: NL
authors.lastname: Baker
authors.affiliation: Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville and Department of Paediatrics, University of Melbourne, Parkville, Australia.
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Miller
authors.affiliation: Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville and Department of Paediatrics, University of Melbourne, Parkville, Australia.
authors.forename: Donald F
authors.initials: DF
authors.lastname: Newgreen
authors.affiliation: Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville and Department of Paediatrics, University of Melbourne, Parkville, Australia. Electronic address: peter.farlie@mcri.edu.au.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Farlie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004622:Embryo, Mammalian; D015870:Gene Expression; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009420:Nervous System / Q000378:metabolism; D009432:Neural Crest / Q000378:metabolism; D054259:Neural Tube / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D057093:Orphan Nuclear Receptors / Q000096:biosynthesis*; D018035:Receptors, Odorant / Q000096:biosynthesis*
chemical_list: D009419:Nerve Tissue Proteins; D057093:Orphan Nuclear Receptors; D018035:Receptors, Odorant
keywords: Embryonic expression; Non-olfactory; Olfactory receptor