title: Lam6 Regulates the Extent of Contacts between Organelles.
abstract: Communication between organelles is crucial for eukaryotic cells to function as one coherent unit. An important means of communication is through membrane contact sites, where two organelles come into close proximity allowing the transport of lipids and small solutes between them. Contact sites are dynamic in size and can change in response to environmental or cellular stimuli; however, how this is regulated has been unclear. Here, we show that Saccharomyces cerevisiae Lam6 resides in several central contact sites: ERMES (ER/mitochondria encounter structure), vCLAMP (vacuole and mitochondria patch), and NVJ (nuclear vacuolar junction). We show that Lam6 is sufficient for expansion of contact sites under physiological conditions and necessary for coordination of contact site size. Given that Lam6 is part of a large protein family and is conserved in vertebrates, our work opens avenues for investigating the underlying principles of organelle communication.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Elbaz-Alon
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Eisenberg-Bord
authors.affiliation: Department of Chemical Research Support, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Vera
authors.initials: V
authors.lastname: Shinder
authors.affiliation: Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung (ZBMZ), Universität Freiburg, Freiburg 79104, Germany; Fakultät für Biologie, Universität Freiburg, Freiburg 79104, Germany.
authors.forename: Sebastian Berthold
authors.initials: SB
authors.lastname: Stiller
authors.affiliation: Department of Chemical Research Support, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Eyal
authors.initials: E
authors.lastname: Shimoni
authors.affiliation: Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung (ZBMZ), Universität Freiburg, Freiburg 79104, Germany; BIOSS Centre for Biological Signalling Studies, Universität Freiburg, Freiburg 79104, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Wiedemann
authors.affiliation: Department of Human Molecular Genetics and Biochemistry, Tel-Aviv University, Tel Aviv 6997801, Israel.
authors.forename: Tamar
authors.initials: T
authors.lastname: Geiger
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address: maya.schuldiner@weizmann.ac.il.
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
mesh_terms: D017920:Antiporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D017920:Antiporters; C000599842:LTC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins