title: An ultralow background substrate for protein microarray technology.
abstract: We herein report an ultralow background substrate for protein microarrays. Conventional protein microarray substrates often suffer from non-specific protein adsorption and inhomogeneous spot morphology. Consequently, surface treatment and a suitable printing solution are required to improve the microarray performance. In the current work, we improved the situation by developing a new microarray substrate based on a fluorinated ethylene propylene (FEP) membrane. A polydopamine microspot array was fabricated on the FEP membrane, with proteins conjugated to the FEP surface through polydopamine. Uniform microspots were obtained on FEP without the application of a special printing solution. The modified FEP membrane demonstrated ultralow background signal and was applied in protein and peptide microarray analysis.
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China. bozheng@cuhk.edu.hk.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ma
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene / Q000031:analogs & derivatives* / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods* / Q000639:trends; D005465:Fluorocarbon Polymers
chemical_list: D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene; C096305:fluorinated ethylene propylene; D005465:Fluorocarbon Polymers