title: Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina.
abstract: OBJECTIVE
  To increase the commercial value of oleaginous fungus Mortierella alpina by incorporation of trans-10,cis-12 conjugated linoleic acid (CLA) into the polyunsaturated fatty acids (PUFAs) of M. alpina via Propionibacterium acnes isomerase (PAI) conversion.
  
  
  RESULTS
  The PAI gene and the codon optimization version were heterologously expressed in M. alpina via Agrobacterium tumefaciens-mediated transformation (ATMT). Coding usage modification significantly improved the translation of PAI transcripts and trans-10,cis-12 CLA was produced up to 1.2 mg l(-1), which corresponds to approx. 0.05% of the total fatty acid (TFA). Since PAI prefers free linoleic acid as a substrate rather than any other forms, 5 μM long-chain acyl CoA synthetase inhibitor was added and the trans-10,cis-12 CLA content increased approx. 24-fold to 29 mg l(-1), reaching up to 1.2% (w/w) of the TFA in M. alpina.
  
  
  CONCLUSIONS
  Heterologous expression of PAI in M. alpina by ATMT methods is a practicable way in biosynthesis of CLA and this system may be a feasible platform for industrial production of CLA.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.
authors.forename: Danhui
authors.initials: D
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China. haiqinchen@jiangnan.edu.cn.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China. haiqinchen@jiangnan.edu.cn.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China.
authors.forename: Guangfei
authors.initials: G
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China.
authors.forename: Baixi
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People's Republic of China.|Departments of Cancer Biology and Biochemistry, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D015870:Gene Expression; D007535:Isomerases / Q000235:genetics / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000378:metabolism*; D020094:Mortierella / Q000235:genetics / Q000378:metabolism*; D011425:Propionibacterium acnes / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D044243:Linoleic Acids, Conjugated; D011994:Recombinant Proteins; D007535:Isomerases
keywords: Conjugated linoleic acid; Heterologous expression; Linoleic acid isomerase: Mortierella alpine; Trans-10,cis-12CLA; Triacsin