title: Advanced colorectal adenoma related gene expression signature may predict prognostic for colorectal cancer patients with adenoma-carcinoma sequence.
abstract: BACKGROUND
  There are still no absolute parameters predicting progression of adenoma into cancer. The present study aimed to characterize functional differences on the multistep carcinogenetic process from the adenoma-carcinoma sequence.
  
  
  METHODS
  All samples were collected and mRNA expression profiling was performed by using Agilent Microarray high-throughput gene-chip technology. Then, the characteristics of mRNA expression profiles of adenoma-carcinoma sequence were described with bioinformatics software, and we analyzed the relationship between gene expression profiles of adenoma-adenocarcinoma sequence and clinical prognosis of colorectal cancer.
  
  
  RESULTS
  The mRNA expressions of adenoma-carcinoma sequence were significantly different between high-grade intraepithelial neoplasia group and adenocarcinoma group. The biological process of gene ontology function enrichment analysis on differentially expressed genes between high-grade intraepithelial neoplasia group and adenocarcinoma group showed that genes enriched in the extracellular structure organization, skeletal system development, biological adhesion and itself regulated growth regulation, with the P value after FDR correction of less than 0.05. In addition, IPR-related protein mainly focused on the insulin-like growth factor binding proteins.
  
  
  CONCLUSIONS
  The variable trends of gene expression profiles for adenoma-carcinoma sequence were mainly concentrated in high-grade intraepithelial neoplasia and adenocarcinoma. The differentially expressed genes are significantly correlated between high-grade intraepithelial neoplasia group and adenocarcinoma group. Bioinformatics analysis is an effective way to study the gene expression profiles in the adenoma-carcinoma sequence, and may provide an effective tool to involve colorectal cancer research strategy into colorectal adenoma or advanced adenoma.
authors.affiliation: Department of Surgical Oncology, Ward 3rd, Beijing Shijitan Hospital, Capital Medical University, Capital Medical University School of Oncology, Peking University Ninth School of Clinical Medicine Beijing 100038, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100021, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Shi
authors.affiliation: Department of Surgical Oncology, Ward 3rd, Beijing Shijitan Hospital, Capital Medical University, Capital Medical University School of Oncology, Peking University Ninth School of Clinical Medicine Beijing 100038, China.
authors.forename: Dai-Xiang
authors.initials: DX
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100021, China.
authors.forename: Bang-Rong
authors.initials: BR
authors.lastname: Cao
authors.affiliation: Department of Surgical Oncology, Ward 3rd, Beijing Shijitan Hospital, Capital Medical University, Capital Medical University School of Oncology, Peking University Ninth School of Clinical Medicine Beijing 100038, China.
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Luo
authors.affiliation: Department of Surgical Oncology, Ward 3rd, Beijing Shijitan Hospital, Capital Medical University, Capital Medical University School of Oncology, Peking University Ninth School of Clinical Medicine Beijing 100038, China.
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Cheng
keywords: Colorectal cancer; adenoma-carcinoma sequence; advanced colorectal adenoma; mRNA expression profile; prognosis