title: Selenium protects cerebral cells by cisplatin induced neurotoxicity.
abstract: OBJECTIVE
  To evaluate the central nervous system toxicity of cisplatin and neuroprotective effect of selenium.
  
  
  METHODS
  Twenty-one male Wistar albino rats were divided into three groups: control (C), cisplatin (CS), cisplatin and selenium (CSE, n=7 in each group). Cisplatin (12 mg/kg/day, i.p.) was administered to CS and CSE groups for three days. Furthermore, CSE group received 3mg/kg/day (twice-a-day as 1.5 mg/kg) selenium via oral gavage five days before cisplatin injection and continued for 11 consecutive days. The same volumes of saline were administered to C group intraperitoneally and orally at same time.
  
  
  RESULTS
  Heterochromatic and vacuolated neurons and dilated capillary vessels in the brain were observed in the histochemical examinations of cisplatin treated group. Rats that were given a dose of 3mg/kg/day selenium decreased the cisplatin induced histopathological changes in the brain, indicating a protective effect. In addition, cytoplasmic staining of the cell for bcl-2, both cytoplasmic and nuclear staining for bax were determined to be positive in the all groups. Bax positive cells were increased in the CS group compared to C group, in contrast to decreased bcl-2 positivity.
  
  
  CONCLUSIONS
  Selenium limited apototic activity and histological changes due to the cisplatin related central neurotoxicity.
authors.affiliation: Department of Neurosurgery, Afyon Kocatepe University, Afyonkarahisar, Turkey.
authors.forename: Ergun
authors.initials: E
authors.lastname: Karavelioglu
authors.affiliation: Department of Neurosurgery, Afyon Kocatepe University, Turkey.
authors.forename: Mehmet Gazi
authors.initials: MG
authors.lastname: Boyaci
authors.affiliation: Department of Histology and Embryology, Balikesir University, Balikesir, Turkey.
authors.forename: Nejdet
authors.initials: N
authors.lastname: Simsek
authors.affiliation: Department of Neurosurgery, Balikesir University, Balikesir, Turkey.
authors.forename: Mehmet Akif
authors.initials: MA
authors.lastname: Sonmez
authors.affiliation: Department of Neurology, Balikesir University, Balikesir, Turkey.
authors.forename: Rabia
authors.initials: R
authors.lastname: Koc
authors.affiliation: Department of Neurosurgery, Afyonkarahisar State Hospital, Afyonkarahisar, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Karademir
authors.affiliation: Department of Neurosurgery, Canakkale 18 Mart University, Canakkale, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Guven
authors.affiliation: Department of Neurosurgery, Balikesir University, Balikesir, Turkey.
authors.forename: Olcay
authors.initials: O
authors.lastname: Eser
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002945:Cisplatin / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012643:Selenium / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D018696:Neuroprotective Agents; D012643:Selenium; D002945:Cisplatin