title: Phylogenetic analysis of the complete mitogenome sequence of the raspberry weevil, Aegorhinus superciliosus (Coleoptera: Curculionidae), supports monophyly of the tribe Aterpini.
abstract: The superfamily Curculionoidea is one of the most diverse groups of insects in the world, including many species which are crop pests. Within this group, the native raspberry weevil, Aegorhinus superciliosus (Guérin, 1830), is an important pest in blueberry and raspberry fields in southern South America. Using a 454 sequencing approach, we sequenced and annotated the mitogenome of A. superciliosus, it, providing the first such information for any species in the tribe Aterpini, subfamily Cyclominae. The assembled mitogenome is a circular DNA molecule 15,121bp in length containing all 37 genes normally found in metazoans. Mitogenome organization and transcriptional orientation in A. superciliosus showed the same pattern that characterizes the suborder Polyphaga. Bayesian and Maximum Likelihood phylogenetic analyses supported the monophyly of the tribe Aterpini and the subfamily Cyclominae, recovering this clade in a sister group relationship with Entiminae and Hyperinae. The monophyly of these three subfamilies defines a critical transition to an ectophagous lifestyle in the larvae, from an ancestrally endophagous larval lifestyle in all other lineages. The sequenced mitogenome of A. superciliosus can provide basic data for future studies investigating population history, molecular systematics, stress ecophysiology and phylogeography.
authors.affiliation: Departamento de Producción Agrícola, Facultad de Ciencias Agrarias, Universidad de Talca, Casilla 747, Talca, Chile; Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile, Casilla 567, Valdivia, Chile. Electronic address: mcabrerabrandt@gmail.com.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Cabrera-Brandt
authors.affiliation: Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile, Casilla 567, Valdivia, Chile; CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart 7001, TAS, Australia.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Gaitán-Espitia
mesh_terms: D000818:Animals; D017344:Genes, Insect; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D065856:Rubus / Q000469:parasitology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D048889:Weevils / Q000145:classification* / Q000235:genetics*
keywords: Comparative phylogenetics; Curculionoidea; Ectophagy; Mitogenome; Weevils