title: Quantitation of the Regional Lymph Node Metastatic Burden and Prognosis in Malignant Mammary Tumors of Dogs.
abstract: BACKGROUND
  As in women, regional lymph node status impacts survival in dogs with malignant mammary tumors. However, few studies have evaluated regional lymph node metastases in dogs with malignant mammary gland tumors.
  
  
  OBJECTIVE
  To estimate overall survival based on the assessments of the lymph node status and the morphologic and morphometric features in female dogs with malignant mammary gland tumors.
  
  
  METHODS
  In total, 178 lymph nodes from 97 female dogs were assessed and reviewed, and after confirmation by immunohistochemistry (IHC), 161 lymph nodes were selected for analysis of metastases. Animals were considered metastasis-free (negative lymph nodes) only after IHC analysis for cytokeratin AE1/AE3. The number of positive lymph nodes, the number of metastatic foci, the maximum diameter and the area of metastasis were analyzed, and estimates of overall survival were made.
  
  
  RESULTS
  Dogs with metastasis had lower mean survival than those with metastasis-free regional lymph nodes, showing a direct relationship between the number of affected lymph nodes and shorter survival. However, histologic analysis of the lymph nodes identified lower survival rates in animals with macrometastases and isolated tumor cells, areas of metastasis >20.11 mm², and metastatic diameters >7.32 mm.
  
  
  CONCLUSIONS
  The identification of ≥1 lymph nodes positive for metastasis and morphometric characterization of lymphatic metastases indicate the prognostic relevance of lymph nodes status in dogs with mammary tumors.
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil.
authors.forename: M R
authors.initials: MR
authors.lastname: de Araújo
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil.
authors.forename: L C
authors.initials: LC
authors.lastname: Campos
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Ferreira
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil.
authors.forename: G D
authors.initials: GD
authors.lastname: Cassali
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology; D004285:Dogs; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008321:Mammary Glands, Animal / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000401:mortality / Q000473:pathology*; D011379:Prognosis
keywords: Canine; Lymph node; Mammary tumors; Metastasis; Survival