title: Protective Effect of Neuropeptide Substance P on Bone Marrow Mesenchymal Stem Cells against Apoptosis Induced by Serum Deprivation.
abstract: Substance P (SP) contributes to bone formation by stimulating the proliferation and differentiation of bone marrow stromal cells (BMSCs); however, the possible involved effect of SP on apoptosis induced by serum deprivation (SD) in BMSCs is unclear. To explore the potential protective effect of SP and its mechanism, we investigated the relationships among SP, apoptosis induced by SD, and Wnt signaling in BMSCs. SP exhibited a protective effect, as indicated by a reduction in the apoptotic rate, nuclear condensation, caspase-3 and caspase-9 activation, and the ratio of Bax/Bcl-2 that was observed after 24 h of SD. This protective effect was blocked by the inhibition of Wnt signaling or antagonism of the NK-1 receptor. Moreover, SP promoted the mRNA and protein expression of Wnt signaling molecules such as β-catenin, p-GSK-3β, c-myc, and cyclin D1 in addition to the nuclear translocation of β-catenin, indicating that active Wnt signaling is involved in SP inhibition of apoptosis. Our results revealed that mediated by the NK-1 receptor, SP exerts an inhibitory effect on serum deprivation induced apoptosis in BMSCs that is related to the activation of canonical Wnt signaling.
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Su
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Jin
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Engineering and Materials Science, Queen Mary University of London, London, UK.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Xiangyang Central Hospital, Xiangyang, Hubei 441021, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Mei
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Zhen-Lv
authors.initials: ZL
authors.lastname: Zou
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Jian-Qun
authors.initials: JQ
authors.lastname: Wu
authors.affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.
authors.forename: Zi-Yi
authors.initials: ZY
authors.lastname: Xu