title: Low expression of microRNA-204 (miR-204) is associated with poor clinical outcome of acute myeloid leukemia (AML) patients.
abstract: BACKGROUND
  Acute myeloid leukemia (AML) is a heterogeneous neoplasm of the bone marrow with poor prognosis. In clinical practice new prognostic factors are still needed. MicroRNAs (miRs), small endogenous noncoding RNAs, play an essential role in the development and progression of acute leukemia. The aim of the study was to evaluate miR-204 expression in patients with AML at diagnosis and after induction chemotherapy, in comparison to healthy controls. We also investigated, if miR-204 expression correlates with clinical features of AML patients.
  
  
  METHODS
  miR-204 expression has been analyzed using RT-PCR in 95 bone marrow specimens from newly diagnosed AML patients in comparison to 20 healthy subject.
  
  
  RESULTS
  We showed down-regulated miR-204 expression in AML patients, which was associated with shorter patients' survival. Higher expression of miR-204 in patients after induction therapy was correlated with complete remission achieving.
  
  
  CONCLUSIONS
  We showed low miR-204 expression in AML and found it to be an independent prognostic factor in this patient population.
authors.affiliation: Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Wroclaw Medical University, Pasteur 4 Str, 50-367, Wroclaw, Poland. aleksandra.butrym@gmail.com.|Department of Physiology, Wroclaw Medical University, Wroclaw, Poland. aleksandra.butrym@gmail.com.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Butrym
authors.affiliation: Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Wroclaw Medical University, Pasteur 4 Str, 50-367, Wroclaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Rybka
authors.affiliation: Department of Forensic Medicine, Molecular Techniques Unit, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Baczyńska
authors.affiliation: Department of Epidemiology, Cancer Center-Institute of Oncology, Gliwice, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tukiendorf
authors.affiliation: Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Wroclaw Medical University, Pasteur 4 Str, 50-367, Wroclaw, Poland.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kuliczkowski
authors.affiliation: Department of Internal and Occupational Diseases and Hypertension, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Mazur
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000401:mortality; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D016019:Survival Analysis; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D035683:MicroRNAs