title: Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin.
abstract: The effect of homogeneous fibrin (Fb), collagen (Coll) and composite fibrin-heparin (Fb-Hp), fibrin-collagen (Fb-Coll) membranes on in vitro release of platelet-derived growth factor (PDGF-BB) was evaluated in the presence or absence of amoxicillin using of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 272 nm. The process of the PDGF-BB growth factor and amoxicillin release from the studied membranes was of a two-phase nature in the majority of the systems analysed. The PDGF-BB was released in the highest amount from the Coll membrane (M7) without the presence of amoxicillin--546.2 ± 7.47 pg, t0.5 = 0.88 h and 202.5 ± 6.83 pg, t0.5 = 26.65 h during the first phase and second phase, respectively. The lowest PDGF-BB release was observed from composite M4 (Fb-Hp) membrane--5.88 ± 0.81 pg, t0.5 = 1.69 h; and 110.2 ± 6.48 pg, t0.5 = 855.6 h during first and second phase respectively. An optimal release of amoxicillin was observed in the case of the composite M6 (Fb-Coll) membrane--only in the second phase: 64.2 ± 7.8 μg, t0.5 = 83.5 h. The lowest and delayed amoxicillin release was achieved for M4 membrane (approx. 17.1 ± 1.12 μg, t0.5 = 46.5 h). The results of the PDGF-BB release and amoxicillin from membranes indicated a correlation between the level of release and composition of the film. Our results suggested that fibrin and collagen membranes may be beneficial to enhance periodontal bone regeneration.
authors.forename: Marta
authors.initials: M
authors.lastname: Michalska
authors.forename: Marcin
authors.initials: M
authors.lastname: Kozakiewicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bodek
authors.forename: Kazimiera Henryka
authors.initials: KH
authors.lastname: Bodek
mesh_terms: D000658:Amoxicillin / Q000008:administration & dosage*; D000077214:Becaplermin; D003094:Collagen / Q000008:administration & dosage*; D005337:Fibrin / Q000008:administration & dosage*; D006801:Humans; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D000658:Amoxicillin; D005337:Fibrin; D003094:Collagen