title: Conferring high-temperature tolerance to nontransgenic tomato scions using graft transmission of RNA silencing of the fatty acid desaturase gene.
abstract: We investigated graft transmission of high-temperature tolerance in tomato scions to nontransgenic scions from transgenic rootstocks, where the fatty acid desaturase gene (LeFAD7) was RNA-silenced. Tomato was transformed with a plasmid carrying an inverted repeat of LeFAD7 by Agrobacterium. Several transgenic lines showed the lower amounts of LeFAD7 RNA and unsaturated fatty acids, while nontransgenic control did not, and siRNA was detected in the transgenic lines, but not in control. These lines grew under conditions of high temperature, while nontransgenic control did not. Further, the nontransgenic plants were grafted onto the silenced transgenic plants. The scions showed less of the target gene RNA, and siRNA was detected. Under high-temperature conditions, these grafted plants grew, while control grafted plants did not. Thus, it was shown that high-temperature tolerance was conferred in the nontransgenic scions after grafting onto the silenced rootstocks.
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Hondo
authors.affiliation: Berg Earth Inc, Uwajima, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kawara
authors.affiliation: Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
authors.forename: Yozo
authors.initials: Y
authors.lastname: Okazaki
authors.affiliation: Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.|Graduate School of Pharmaceutical Sciences, Chiba University, Inage-ku, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Kappei
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaeno
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamaoka
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nishiguchi
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D001483:Base Sequence; D044943:Fatty Acid Desaturases / Q000235:genetics*; D017343:Genes, Plant; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D034622:RNA Interference; D046509:Tissue Culture Techniques / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D044943:Fatty Acid Desaturases
keywords: LeFAD7; RNA silencing; fatty acid desaturase; graft transmission; high-temperature tolerance; tomato