title: A red-emitting ratiometric fluorescent probe based on a benzophosphole P-oxide scaffold for the detection of intracellular sodium ions.
abstract: We disclose the development of a ratiometric fluorescent probe based on a benzophosphole P-oxide and its application for the detection of intracellular Na(+) ions. Excitation by visible light induced red emission from this probe in water, which was subjected to a hypsochromic shift upon complexation with Na(+). Based on this change, a ratiometric analysis enabled us to visualise changes in the Na(+) concentration in living mammalian cells.
authors.affiliation: Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. taki@itbm.nagoya-u.ac.jp yamaguchi@chem.naogya-u.ac.jp.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ogasawara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Osaki
authors.forename: Aiko
authors.initials: A
authors.lastname: Fukazawa
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Sato
authors.forename: Kimi
authors.initials: K
authors.lastname: Ogasawara
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higashiyama
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Yamaguchi
mesh_terms: D003498:Cyclic P-Oxides / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D015394:Molecular Structure; D010087:Oxides / Q000138:chemical synthesis / Q000737:chemistry*; D012964:Sodium / Q000032:analysis*
chemical_list: D003498:Cyclic P-Oxides; D005456:Fluorescent Dyes; D007477:Ions; D010087:Oxides; D012964:Sodium