title: Caudate neuronal recording in freely behaving animals following acute and chronic dose response methylphenidate exposure.
abstract: The misuse and abuse of the psychostimulant, methylphenidate (MPD) the drug of choice in the treatment of attention deficit hyperactivity disorder (ADHD) has seen a sharp uprising in recent years among both youth and adults for its cognitive enhancing effects and for recreational purposes. This uprise in illicit use has lead to many questions concerning the long-term consequences of MPD exposure. The objective of this study was to record animal behavior concomitantly with the caudate nucleus (CN) neuronal activity following acute and repetitive (chronic) dose response exposure to methylphenidate (MPD). A saline control and three MPD dose (0.6, 2.5, and 10.0mg/kg) groups were used. Behaviorally, the same MPD dose in some animals following chronic MPD exposure elicited behavioral sensitization and other animals elicited behavioral tolerance. Based on this finding, the CN neuronal population recorded from animals expressing behavioral sensitization was also evaluated separately from CN neurons recorded from animals expressing behavioral tolerance to chronic MPD exposure, respectively. Significant differences in CN neuronal population responses between the behaviorally sensitized and the behaviorally tolerant animals were observed for the 2.5 and 10.0mg/kg MPD exposed groups. For 2.5mg/kg MPD, behaviorally sensitized animals responded by decreasing their firing rates while behaviorally tolerant animals showed mainly an increase in their firing rates. The CN neuronal responses recorded from the behaviorally sensitized animals following 10.0mg/kg MPD responded by increasing their firing rates whereas the CN neuronal recordings from the behaviorally tolerant animals showed that approximately half decreased their firing rates in response to 10.0mg/kg MPD exposure. The comparison of percentage change in neuronal firing rates showed that the behaviorally tolerant animals trended to exhibit increases in their neuronal firing rates at ED1 following initial MPD exposure and oppositely at ED10 MPD rechallenge. While the behaviorally sensitized animals in general increased in their percentage change of firing rats were observed following acute 10.0mg/kg MPD and the behaviorally sensitized 10.0mg/kg MPD animals and a robust increase in neuronal firing rates at ED1 and ED10 rechallenge. These results suggest the need to first individually analyze animal behavioral activity, and then to evaluate the neuronal responses to the drug based on the animals behavioral response to chronic MPD exposure.
authors.affiliation: University of Texas Health Science Center Medical School at Houston, 6431 Fannin St., MSB 7.208B, Houston, TX 77030, USA.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Claussen
authors.affiliation: University of Texas Health Science Center Medical School at Houston, 6431 Fannin St., MSB 7.208B, Houston, TX 77030, USA. Electronic address: Nachum.dafny@uth.tmc.edu.
authors.forename: Nachum
authors.initials: N
authors.lastname: Dafny
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002421:Caudate Nucleus / Q000187:drug effects* / Q000502:physiology*; D059333:Central Nervous System Sensitization / Q000187:drug effects; D000697:Central Nervous System Stimulants / Q000008:administration & dosage* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance / Q000502:physiology; D008774:Methylphenidate / Q000008:administration & dosage* / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000697:Central Nervous System Stimulants; D008774:Methylphenidate
keywords: Behavior; Caudate nucleus; Dose–response; Electrophysiology; Ritalin; Sensitized/tolerance