title: Toward the Identification of Two Glycoproteins Involved in the Stomatal Deregulation of Downy Mildew-Infected Grapevine Leaves.
abstract: Stomata remain abnormally opened and unresponsive to abscisic acid in grapevine leaves infected by downy mildew. This deregulation occurs from 3 days postinoculation and increases concomitantly with leaf colonization by the pathogen. Using epidermal peels, we demonstrated that the active compound involved in this deregulation is located in the apoplast. Biochemical assays showed that the active compound present in the apoplastic fluids isolated from Plasmopara viticola-infected grapevine leaves (IAF) is a CysCys bridge-independent, thermostable and glycosylated protein. Fractionation guided assays based on chromatography coupled to stomatal response and proteomic analysis allowed the identification of both plant and pathogen proteins in the active fraction obtained from IAF. Further in silico analysis and discriminant filtrations based on the comparison between predictions and experimental indications lead to the identification of two Vitis vinifera proteins as candidates for the observed stomatal deregulation.
authors.affiliation: 1 CNRS, UMR1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21000 Dijon, France;
authors.forename: Christelle
authors.initials: C
authors.lastname: Guillier
authors.affiliation: 2 Université de Bourgogne, UMR1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21000 Dijon, France;
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gamm
authors.affiliation: 3 Clinical Innovation Proteomic Platform - CLIPP, 15 Boulevard Maréchal de Lattre de Tassigny, BP37013, F-21070 Dijon cedex, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Lucchi
authors.affiliation: 3 Clinical Innovation Proteomic Platform - CLIPP, 15 Boulevard Maréchal de Lattre de Tassigny, BP37013, F-21070 Dijon cedex, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Truntzer
authors.affiliation: 3 Clinical Innovation Proteomic Platform - CLIPP, 15 Boulevard Maréchal de Lattre de Tassigny, BP37013, F-21070 Dijon cedex, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Pecqueur
authors.affiliation: 3 Clinical Innovation Proteomic Platform - CLIPP, 15 Boulevard Maréchal de Lattre de Tassigny, BP37013, F-21070 Dijon cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ducoroy
authors.affiliation: 2 Université de Bourgogne, UMR1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21000 Dijon, France;
authors.forename: Marielle
authors.initials: M
authors.lastname: Adrian
authors.affiliation: 2 Université de Bourgogne, UMR1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21000 Dijon, France;
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Héloir
mesh_terms: D000595:Amino Acid Sequence; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D002852:Chromatography, Ion Exchange; D003198:Computer Simulation; D005656:Fungal Proteins / Q000378:metabolism; D006023:Glycoproteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D009868:Oomycetes / Q000378:metabolism* / Q000502:physiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D040901:Proteomics / Q000379:methods; D017386:Sequence Homology, Amino Acid; D027843:Vitis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins; D010940:Plant Proteins