title: Methodological challenges of optical tweezers-based X-ray fluorescence imaging of biological model organisms at synchrotron facilities.
abstract: Recently, a radically new synchrotron radiation-based elemental imaging approach for the analysis of biological model organisms and single cells in their natural in vivo state was introduced. The methodology combines optical tweezers (OT) technology for non-contact laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time at ESRF-ID13. The optical manipulation possibilities and limitations of biological model organisms, the OT setup developments for XRF imaging and the confocal XRF-related challenges are reported. In general, the applicability of the OT-based setup is extended with the aim of introducing the OT XRF methodology in all research fields where highly sensitive in vivo multi-elemental analysis is of relevance at the (sub)micrometre spatial resolution level.
authors.affiliation: Department of Analytical Chemistry, Ghent University, Krijgslaan 281, Ghent 9000, Belgium.
authors.forename: Eva
authors.initials: E
authors.lastname: Vergucht
authors.affiliation: Department of Electronics and Information Systems, Ghent University, Sint-Pietersnieuwstraat 41, Ghent 9000, Belgium.
authors.forename: Toon
authors.initials: T
authors.lastname: Brans
authors.affiliation: Department of Electronics and Information Systems, Ghent University, Sint-Pietersnieuwstraat 41, Ghent 9000, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Beunis
authors.affiliation: Department of Analytical Chemistry, Ghent University, Krijgslaan 281, Ghent 9000, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Garrevoet
authors.affiliation: Department of Analytical Chemistry, Ghent University, Krijgslaan 281, Ghent 9000, Belgium.
authors.forename: Stephen
authors.initials: S
authors.lastname: Bauters
authors.affiliation: Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Jozef Plateaustraat 22, Ghent 9000, Belgium.
authors.forename: Maarten
authors.initials: M
authors.lastname: De Rijcke
authors.affiliation: Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Jozef Plateaustraat 22, Ghent 9000, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Deruytter
authors.affiliation: Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Jozef Plateaustraat 22, Ghent 9000, Belgium.
authors.forename: Colin
authors.initials: C
authors.lastname: Janssen
authors.affiliation: European Synchrotron Radiation Facility, 71 avenue des Martyrs, Grenoble 38000, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Riekel
authors.affiliation: Department of Analytical Chemistry, Ghent University, Krijgslaan 281, Ghent 9000, Belgium.
authors.forename: Manfred
authors.initials: M
authors.lastname: Burghammer
authors.affiliation: Department of Analytical Chemistry, Ghent University, Krijgslaan 281, Ghent 9000, Belgium.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Vincze
mesh_terms: D008954:Models, Biological; D052898:Optical Tweezers; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D017356:Synchrotrons
keywords: X-ray fluorescence; confocal XRF; optical tweezers; single-cell imaging