title: Correlating 3D morphology with molecular pathology: fibrotic remodelling in human lung biopsies.
abstract: Assessing alterations of the parenchymal architecture is essential in understanding fibrosing interstitial lung diseases. Here, we present a novel method to visualise fibrotic remodelling in human lungs and correlate morphological three-dimensional (3D) data with gene and protein expression in the very same sample. The key to our approach is a novel embedding resin that clears samples to full optical transparency and simultaneously allows 3D laser tomography and preparation of sections for histology, immunohistochemistry and RNA isolation. Correlating 3D laser tomography with molecular diagnostic techniques enables new insights into lung diseases. This approach has great potential to become an essential tool in pulmonary research.
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Kellner
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Institute for Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Wehling
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Hannover, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Warnecke
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Marko
authors.initials: M
authors.lastname: Heidrich
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Institute for Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Izykowski
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Department of Radiology, Hannover Medical School, Hannover, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Vogel-Claussen
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Raoul-Amadeus
authors.initials: RA
authors.lastname: Lorbeer
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Georgios
authors.initials: G
authors.lastname: Antonopoulos
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Department of Experimental Pneumology, Hannover Medical School, Hannover, Germany REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Sabina
authors.initials: S
authors.lastname: Janciauskiene
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Grothausmann
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Knudsen
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Tammo
authors.initials: T
authors.lastname: Ripken
authors.affiliation: Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Hannover, Germany Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Meyer
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Institute for Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Kreipe
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Ochs
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany Institute for Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Danny
authors.initials: D
authors.lastname: Jonigk
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Mark Philipp
authors.initials: MP
authors.lastname: Kühnel
mesh_terms: D005355:Fibrosis; D006801:Humans; D021621:Imaging, Three-Dimensional; D008168:Lung / Q000473:pathology*; D014057:Tomography, X-Ray Computed / Q000379:methods*
keywords: Allergic Alveolitis; Imaging/CT MRI etc; Interstitial Fibrosis