title: Beneficial Effects of Polygonum multiflorum on Hippocampal Neuronal Cells and Mouse Focal Cerebral Ischemia.
abstract: Beneficial effects of the water extract of Polygonum multiflorum (WEPM) and their mechanisms were investigated in HT22 hippocampal cells and hippocampus of middle cerebral artery occlusion (MCAO) mice. In HT22 cells against glutamate-induced oxidative stress, pretreatment with WEPM resulted in significantly reduced apoptotic neuronal death. Pretreatment with WEPM resulted in the suppression of ROS accumulation in connection with cellular Ca (2+) level after exposure to glutamate. Treatment with glutamate alone led to suppressed protein level of mature brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element binding protein (CREB); however, pretreatment with either WEPM or anti-oxidant N-acetyl-ʟ-cysteine (NAC) resulted in the significant enhancement of levels of these proteins. In addition, levels of mature BDNF expression and CREB phosphorylation were increased by combined treatment with WEPM, NAC, and intracellular Ca (2+) inhibitor BAPTA compared to other treatment groups. In MCAO mice, we confirmed the critical role of mature BDNF expression and CREB phosphorylation by WEPM in the neurons of the hippocampus. Our results suggest that WEPM mainly exerted beneficial effects on hippocampal neurons through the suppression of ROS accumulation and up-regulation of mature BDNF expression and CREB phosphorylation.
authors.affiliation: Department of Korean Medical Science, Pusan National University, Yangsan 626-870, Republic of Korea.|Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Ahn
authors.affiliation: Department of Korean Medical Science, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Yu Ri
authors.initials: YR
authors.lastname: Kim
authors.affiliation: Department of Korean Medical Science, Pusan National University, Yangsan 626-870, Republic of Korea.|Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Ha Neui
authors.initials: HN
authors.lastname: Kim
authors.affiliation: Division of Meridian and Structural Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.|Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Hwa Kyoung
authors.initials: HK
authors.lastname: Shin
authors.affiliation: Department of Korean Medical Science, Pusan National University, Yangsan 626-870, Republic of Korea.|Division of Meridian and Structural Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.|Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Byung Tae
authors.initials: BT
authors.lastname: Choi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004195:Disease Models, Animal; D000068676:Fallopia multiflora / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D018698:Glutamic Acid / Q000633:toxicity; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014867:Water
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017362:Cyclic AMP Response Element-Binding Protein; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014867:Water; D018698:Glutamic Acid; D002118:Calcium
keywords: Focal Cerebral Ischemia; Hippocampal Neuron; Oxidative Stress; Polygonum multiflorum