title: PP073. Gestational hypertension: A study on placental expression of endothelial and inducible nitric oxide synthase and no metabolism.
abstract: BACKGROUND
  Hypertension is one of the most common medical disorders in pregnancy and a major cause of maternal and perinatal morbidity and death. In primates adequate development of the embryo, and later of the fetus, depends on a successful hemomonochorial placentation. Nitric oxide (NO) a low molecular weight mediator, induces vasodilatation, inhibits platelet aggregation, and prevents the adhesion of platelets to endothelial cells. Till date, no data are available regarding gestational hypertension (GH) placenta and no metabolism and related enzyme expression and activity.
  
  
  OBJECTIVE
  The present study aimed to evaluate eNOS and iNOS expression in the placentas of both normal and GH patients, by means of Real-Time quantitative PCR, measure placental nitric oxide and peroxynitrite levels in the same group of subjects, and correlate such findings with HELLP group already published.
  
  
  METHODS
  Fifteen patients with gestational hypertension and thirty healthy pregnant controls comparable for maternal and gestational age were enrolled in the study. Placental tissue was taken immediately after delivery. eNOS and iNOS mRNA levels were evaluated Real-Time quantitative PCR, whereas nitric oxide and peroxynitrite production was measured by a commercially available kit.
  
  
  RESULTS
  Placental eNOS and iNOS mRNA levels were significantly reduced in GH (2,02-fold reduction and 2,33-fold reduction, respectively) when compared to controls. Conversely, NO and ONOO(-) production were significantly higher in GH group compared to control group (31.56±4.15nmol NO/mg prot vs. 23.98±5.14nmol NO/mg prot and 68.49±8.57 arbitrary fluorescence units vs 17.31±2.25 arbitrary fluorescence units; p<0, 05). Such results were compared to HELLP group obtained in an already published study.
  
  
  CONCLUSIONS
  As from results herein reported, we can hypothesize that complex mechanisms involving NO pathways cause a placental vasculature damage. However, it is not easy to understand if these changes could be interpreted as causes or consequences of this pathologic state.
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mazzanti
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vignini
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Nanetti
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Cecati
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Raffaelli
authors.affiliation: Department of Clinical Sciences, Section of Woman Health Science, Università Politecnica Marche, Ancona, Italy.
authors.forename: S R
authors.initials: SR
authors.lastname: Giannubilo
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Emanuelli
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry and Biology, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Saccucci
authors.affiliation: Department of Clinical Sciences, Section of Woman Health Science, Università Politecnica Marche, Ancona, Italy.
authors.forename: A L
authors.initials: AL
authors.lastname: Tranquilli