title: Bone Morphogenetic Protein-2 regulates in vitro osteogenic differentiation of mouse adipose derived stem cells.
abstract: OBJECTIVE
  The aim of this study is to investigate the feasibility and efficiency of Bone Morphogenetic Protein-2 (BMP-2) in regulating in vitro osteogenic differentiation of mouse adipose derived stem cells (ADSCs).
  
  
  METHODS
  Mouse ADSCs were isolated from adipose tissues of C57/BL6 mice (age of 4-6 w) and cultured. Surface antigens of passage 3 (P3) ADSCs, including CD31, CD34, CD90, CD105 and CD133, were analyzed using flow cytometry. Overexpression of BMP-2 was achieved through gene transfection of ADSCs. In vitro osteogenic differentiation of transfected and non-transfected ADSCs cultured in specific induction media was evaluated by Alizarin Red staining. In addition, expression of osteoblast-specific gene, Runx2, was analyzed by quantitative RT-PCR (qRT-PCR).
  
  
  RESULTS
  Abundant ADSCs could be isolated from adipose tissue. P3 ADSCs expressed stem cell-specific molecular markers, CD90 and CD105 but did not express CD31, CD34 or CD133. BMP-2 could efficiently transfect mouse ADSCs. Alizarin Red staining revealed that more calcified nodules were formed in BMP-2 transfected ADSCs. qRT-PCR further confirmed higher level of Runx2 expression in BMP-2 transfected ADSCs (p < 0.05).
  
  
  CONCLUSIONS
  BMP-2 can promote in vitro osteogenic differentiation of mouse adipose stem cells.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China. Zhoujs12399@163.com.
authors.forename: X-D
authors.initials: XD
authors.lastname: Chen
authors.forename: M
authors.initials: M
authors.lastname: Deng
authors.forename: J-S
authors.initials: JS
authors.lastname: Zhou
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Xiao
authors.forename: X-S
authors.initials: XS
authors.lastname: Zhou
authors.forename: C-C
authors.initials: CC
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Wu
authors.forename: Z-D
authors.initials: ZD
authors.lastname: Wang
authors.forename: X-T
authors.initials: XT
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; D005434:Flow Cytometry; D066298:In Vitro Techniques; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; C493716:Runx2 protein, mouse