title: PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice.
abstract: 64Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold.
authors.affiliation: Structural Biophysics, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, DK-2100 København Ø, Denmark.
authors.forename: Pie
authors.initials: P
authors.lastname: Huda
authors.affiliation: Cluster for Molecular Imaging and Department of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet and University of Copenhagen, Blegdamsvej 3, DK-2200 København N, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Binderup
authors.affiliation: Structural Biophysics, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, DK-2100 København Ø, Denmark.
authors.forename: Martin Cramer
authors.initials: MC
authors.lastname: Pedersen
authors.affiliation: Structural Biophysics, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, DK-2100 København Ø, Denmark.
authors.forename: Søren Roi
authors.initials: SR
authors.lastname: Midtgaard
authors.affiliation: Hevesy Laboratory, Center for Nuclear Technology, Technical University of Denmark, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
authors.forename: Dennis Ringkjøbing
authors.initials: DR
authors.lastname: Elema
authors.affiliation: Cluster for Molecular Imaging and Department of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet and University of Copenhagen, Blegdamsvej 3, DK-2200 København N, Denmark.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kjær
authors.affiliation: Hevesy Laboratory, Center for Nuclear Technology, Technical University of Denmark, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
authors.forename: Mikael
authors.initials: M
authors.lastname: Jensen
authors.affiliation: Structural Biophysics, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, DK-2100 København Ø, Denmark.
authors.forename: Lise
authors.initials: L
authors.lastname: Arleth
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003301:Copper Radioisotopes; D004195:Disease Models, Animal; D006573:Heterocyclic Compounds, 1-Ring; D064593:Heterografts; D006801:Humans; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis*; D010316:Particle Size; D010713:Phosphatidylcholines; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D003301:Copper Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D010713:Phosphatidylcholines; D019275:Radiopharmaceuticals; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; C028694:1-palmitoyl-2-oleoylphosphatidylcholine