title: Dynamic compression combined with SOX-9 overexpression in rabbit adipose-derived mesenchymal stem cells cultured in a three-dimensional gradual porous PLGA composite scaffold upregulates HIF-1α expression.
abstract: There is considerable interest in how the fate of adipose-derived stem cells is determined. Physical stimuli play a crucial role in skeletogenesis and in cartilage repair and regeneration. In the present study, we investigated the comparative and interactive effects of dynamic compression and SRY-related high-mobility group box gene-9 (SOX-9) on chondrogenesis of rabbit adipose-derived stem cells in three-dimensional gradual porous PLGA (polylactic-co-glycolic acid) composite scaffolds. Articular cartilage is stratified into zones delineated by characteristic changes in cellular, matrix, and nutritive components. As a consequence, biochemical and biomechanical properties vary greatly between the different zones, giving the tissue its unique structure and, thus, the ability to cope with extreme loading. The effects on development of the cartilage were examined using a combination of computational modeling to predict alterations in biophysical stimuli, detailed morphometric analysis of 3D digital representations. In addition, early chondrogenic differentiation was assessed via real-time PCR of mRNA expression levels for bone- and cartilage-specific gene markers. Our findings define the important role of dynamic compression combined with SOX-9 overexpression during in vitro generation of tissue-engineering cartilage and suggest that a 3D gradual porous PLGA composite scaffold may benefit articular cartilage tissue engineering in cartilage regeneration for better force distribution.
authors.affiliation: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004 Liaoning Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004 Liaoning Province, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004 Liaoning Province, China.
authors.forename: Enbo
authors.initials: E
authors.lastname: Wang
authors.affiliation: Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004 Liaoning Province, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004 Liaoning Province, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Kong
authors.affiliation: Department of Rehabilitation, Shengjing Hospital of China Medical University, Shenyang, 110004 Liaoning Province, China.
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Yuan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology; D002478:Cells, Cultured; D020219:Chondrogenesis; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D055758:SOX9 Transcription Factor / Q000235:genetics*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D015854:Up-Regulation
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D055758:SOX9 Transcription Factor; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: SRY-related high-mobility group box gene-9; adipose-derived stem cells; cartilage; compression; tissue engineering