title: Uranium Biominerals Precipitated by an Environmental Isolate of Serratia under Anaerobic Conditions.
abstract: Stimulating the microbially-mediated precipitation of uranium biominerals may be used to treat groundwater contamination at nuclear sites. The majority of studies to date have focussed on the reductive precipitation of uranium as U(IV) by U(VI)- and Fe(III)-reducing bacteria such as Geobacter and Shewanella species, although other mechanisms of uranium removal from solution can occur, including the precipitation of uranyl phosphates via bacterial phosphatase activity. Here we present the results of uranium biomineralisation experiments using an isolate of Serratia obtained from a sediment sample representative of the Sellafield nuclear site, UK. When supplied with glycerol phosphate, this Serratia strain was able to precipitate 1 mM of soluble U(VI) as uranyl phosphate minerals from the autunite group, under anaerobic and fermentative conditions. Under phosphate-limited anaerobic conditions and with glycerol as the electron donor, non-growing Serratia cells could precipitate 0.5 mM of uranium supplied as soluble U(VI), via reduction to nano-crystalline U(IV) uraninite. Some evidence for the reduction of solid phase uranyl(VI) phosphate was also observed. This study highlights the potential for Serratia and related species to play a role in the bioremediation of uranium contamination, via a range of different metabolic pathways, dependent on culturing or in situ conditions.
authors.affiliation: Research Centre for Radwaste Disposal and Williamson Research Centre, School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom.
authors.forename: Laura
authors.initials: L
authors.lastname: Newsome
authors.affiliation: Research Centre for Radwaste Disposal and Williamson Research Centre, School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom.
authors.forename: Katherine
authors.initials: K
authors.lastname: Morris
authors.affiliation: Research Centre for Radwaste Disposal and Williamson Research Centre, School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Lloyd
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D011232:Chemical Precipitation; D002712:Chlorides / Q000378:metabolism; D003470:Culture Media; D005650:Fumarates / Q000494:pharmacology; D019015:Geologic Sediments / Q000382:microbiology; D005990:Glycerol / Q000494:pharmacology; D008854:Microscopy, Electron; D008903:Minerals / Q000302:isolation & purification / Q000378:metabolism; D053758:Nanoparticles; D010084:Oxidation-Reduction; D010710:Phosphates / Q000302:isolation & purification / Q000378:metabolism; D012705:Serratia / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012990:Soil Pollutants, Radioactive / Q000378:metabolism*; D012995:Solubility; D014501:Uranium / Q000378:metabolism; D017974:Uranium Compounds / Q000302:isolation & purification / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D002712:Chlorides; D003470:Culture Media; D005650:Fumarates; D008903:Minerals; D010710:Phosphates; D012990:Soil Pollutants, Radioactive; D017974:Uranium Compounds; C075963:hydrogen uranyl phosphate; D014501:Uranium; D005990:Glycerol; C047385:uranium oxide; C408075:uranyl chloride