title: Concurrent determination of olanzapine, risperidone and 9-hydroxyrisperidone in human plasma by ultra performance liquid chromatography with diode array detection method: application to pharmacokinetic study.
abstract: A simple and sensitive ultra-performance liquid chromatography (UPLC) method has been developed and validated for simultaneous estimation of olanzapine (OLZ), risperidone (RIS) and 9-hydroxyrisperidone (9-OHRIS) in human plasma in vitro. The sample preparation was performed by simple liquid-liquid extraction technique. The analytes were chromatographed on a Waters Acquity H class UPLC system using isocratic mobile phase conditions at a flow rate of 0.3 mL/min and Acquity UPLC BEH shield RP18 column maintained at 40°C. Quantification was performed on a photodiode array detector set at 277 nm and clozapine was used as internal standard (IS). OLZ, RIS, 9-OHRIS and IS retention times were found to be 0.9, 1.4, .1.8 and 3.1 min, respectively, and the total run time was 4 min. The method was validated for selectivity, specificity, recovery, linearity, accuracy, precision and sample stability. The calibration curve was linear over the concentration range 1-100 ng/mL for OLZ, RIS and 9-OHRIS. Intra- and inter-day precisions for OLZ, RIS and 9-OHRIS were found to be good with the coefficient of variation <6.96%, and the accuracy ranging from 97.55 to 105.41%, in human plasma. The validated UPLC method was successfully applied to the pharmacokinetic study of RIS and 9-OHRIS in human plasma.
authors.affiliation: Department of Pharmaceutical Analysis, PSG College of Pharmacy, Peelamedu, Coimbatore, 641004, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Siva Selva Kumar
authors.affiliation: Department of Pharmacology, PSG College of Pharmacy, Peelamedu, Coimbatore, 641004, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Ramanathan
mesh_terms: D000284:Administration, Oral; D000328:Adult; D001569:Benzodiazepines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D000077152:Olanzapine; D000068882:Paliperidone Palmitate / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D018967:Risperidone / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D055815:Young Adult
chemical_list: D001569:Benzodiazepines; D018967:Risperidone; D000077152:Olanzapine; D000068882:Paliperidone Palmitate
keywords: 9-hydroxyrisperidone; UPLC; olanzapine; pharmacokinetics; risperidone