title: Incidence Changes of Human Papillomavirus in Oropharyngeal Squamous Cell Carcinoma and Effects on Survival in the Netherlands Cancer Institute, 1980-2009.
abstract: OBJECTIVE
  Human papillomavirus (HPV) is a risk factor for oropharyngeal squamous cell carcinoma (OPSCC), with an increasing incidence. The present study aimed to determine the changing incidence of HPV in patients with OPSCC in the period 1980-2009 and its influence on survival.
  
  
  METHODS
  We randomly sampled 158 patients from a cohort of 828 patients with OPSCC stratified by decade (1980-1989, 1990-1999, 2000-2009). Formalin-fixed paraffin-embedded material was tested for HPV DNA by SPF-10 polymerase chain reaction (PCR) and immunohistochemically stained for p16 and p53.
  
  
  RESULTS
  DNA from 146 patients was suitable for HPV detection. HPV DNA was detected in 13/47 (28%), 18/47 (38%), and 20/52 (38%) patients in the cohorts of 1980-1989, 1990-1999, and 2000-2009, respectively (p-value for trend=0.269). Lack of further increase during the most recent decade is inconsistent with the rising incidence and higher prevalence reported in other Western countries. Patients with HPV-positive OPSCC had a better survival in spite of higher tumor stage.
authors.affiliation: Department of Head and Neck Oncology and Surgery, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands r.henneman@nki.nl.
authors.forename: Roel
authors.initials: R
authors.lastname: Henneman
authors.affiliation: Department of Head and Neck Oncology and Surgery, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands Department of Otorhinolaryngology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Hester S
authors.initials: HS
authors.lastname: Van Monsjou
authors.affiliation: Department of Head and Neck Oncology and Surgery, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands Department of Otorhinolaryngology, University Medical Center St Radboud, Nijmegen, the Netherlands.
authors.forename: Caroline V M
authors.initials: CV
authors.lastname: Verhagen
authors.affiliation: Department of Pathology, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
authors.forename: Marie-Louise F
authors.initials: ML
authors.lastname: Van Velthuysen
authors.affiliation: Department of Pathology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Natalja T
authors.initials: NT
authors.lastname: Ter Haar
authors.affiliation: Department of Pathology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: E Michelle
authors.initials: EM
authors.lastname: Osse
authors.affiliation: Department of Biostatistics, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
authors.forename: Marta I
authors.initials: MI
authors.lastname: Lopez-Yurda
authors.affiliation: Department of Head and Neck Oncology and Surgery, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Balm
authors.affiliation: Department of Head and Neck Oncology and Surgery, Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
authors.forename: Michiel W M
authors.initials: MW
authors.lastname: Van Den Brekel
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000209:etiology* / Q000401:mortality / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009426:Netherlands; D009959:Oropharyngeal Neoplasms / Q000209:etiology* / Q000401:mortality / Q000821:virology*; D030361:Papillomavirus Infections / Q000150:complications* / Q000401:mortality / Q000821:virology; D012307:Risk Factors
chemical_list: D004279:DNA, Viral
keywords: HNSCC; HPV incidence; Head and neck squamous cell carcinoma; human papillomavirus; oropharynx; survival