title: Ohr Protects Corynebacterium glutamicum against Organic Hydroperoxide Induced Oxidative Stress.
abstract: Ohr, a bacterial protein encoded by the Organic Hydroperoxide Resistance (ohr) gene, plays a critical role in resistance to organic hydroperoxides. In the present study, we show that the Cys-based thiol-dependent Ohr of Corynebacterium glutamicum decomposes organic hydroperoxides more efficiently than hydrogen peroxide. Replacement of either of the two Cys residues of Ohr by a Ser residue resulted in drastic loss of activity. The electron donors supporting regeneration of the peroxidase activity of the oxidized Ohr of C. glutamicum were principally lipoylated proteins (LpdA and Lpd/SucB). A Δohr mutant exhibited significantly decreased resistance to organic hydroperoxides and marked accumulation of reactive oxygen species (ROS) in vivo; protein carbonylation was also enhanced notably. The resistance to hydrogen peroxide also decreased, but protein carbonylation did not rise to any great extent. Together, the results unequivocally show that Ohr is essential for mediation of organic hydroperoxide resistance by C. glutamicum.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Meiru
authors.initials: M
authors.lastname: Si
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Yaoling
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: Environmental Analytical Laboratory, National Physical & Standards Laboratory, PCSIR, Islamabad, Pakistan.
authors.forename: Muhammad Tausif
authors.initials: MT
authors.lastname: Chaudhry
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000187:drug effects* / Q000502:physiology*; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D050296:Microbial Viability; D008969:Molecular Sequence Data; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010544:Peroxidases