title: Chip-based array magnetic solid phase microextraction on-line coupled with inductively coupled plasma mass spectrometry for the determination of trace heavy metals in cells.
abstract: Determination of trace elements in cells is critical to metallomics research and still faces tremendous difficulties even with the help of highly sensitive inductively coupled plasma mass spectrometry (ICP-MS). Microfluidic chips offer a functional tool-set for cell analysis with features of miniaturization, integration and automation. In this work, we proposed a chip-based array magnetic solid phase microextraction (MSPME) system and on-line combined it with ICP-MS via microflow concentric nebulization for the determination of trace Cu, Zn, Cd, Hg, Pb and Bi in cells. Under the optimized conditions, the limits of detection of the developed on-line chip-based array MSPME-ICP-MS system are 49, 43, 4.2, 6.1, 13 and 18 ng L(-1) for Cu, Zn, Cd, Hg, Pb and Bi, respectively. The proposed method was applied to the analysis of target heavy metals in three kinds of cells (HepG2, Jurkat T and MCF7), and the recoveries for the spiked samples were in the range of 83.8%-117%. The extractable species of the target metals in cells were also studied and the results demonstrated a high selectivity of the developed methods towards free metal ions and/or their small molecular species.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. binhu@whu.edu.cn.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Zhekuan
authors.initials: Z
authors.lastname: Wu
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D019169:Jurkat Cells; D057230:Limit of Detection; D061986:MCF-7 Cells; D013058:Mass Spectrometry; D019216:Metals, Heavy / Q000032:analysis*; D052617:Solid Phase Microextraction
chemical_list: D019216:Metals, Heavy