title: Evaluation of Left Ventricular Regional Systolic Function Using Tissue Doppler Echocardiography After Mesenchymal Stem Cell Transplantation in Rabbits With Myocardial Infarction.
abstract: OBJECTIVE
  The aim of this work was to assess left ventricular (LV) regional systolic function in rabbits with myocardial infarction after allogeneic mesenchymal stem cell (MSC) transplantation using quantitative tissue velocity imaging.
  
  
  METHODS
  Thirty New Zealand White rabbits were assigned into 3 groups randomly: a sham-operated group (n = 10), a myocardial infarction (MI) group (n = 10), and a MSC transplantation group (n = 10). Mesenchymal stem cells (1 × 10(7) in total) were delivered into 5 spots around the left anterior descending artery (LAD) blood supply area via direct intramyocardial injections 1 hour after LAD ligation in the MSC group, whereas the MI group received the same amount of phosphate-buffered saline injections. Echocardiography was performed before LAD ligation and 1 day and 2 weeks after MSC transplantation, respectively. The peak systolic velocity (Vs) of each LV wall segment was measured. The myocardial slices were harvested for histologic staining after the last echocardiographic examination.
  
  
  RESULTS
  The velocity curves for the LV myocardium before LAD ligation had a trend showing that the Vs value decreased gradually from basal to apical segments. The Vs values for the LV segments around the infarcted area in the MSC group decreased significantly compared with the sham group (P < .05) 1 day after MSC transplantation, whereas they increased significantly 2 weeks after MSC transplantation compared with 1 day after LAD ligation (P < .05).
  
  
  CONCLUSIONS
  This study demonstrates that quantitative tissue velocity imaging may provide a promising approach to quantitatively assessing LV regional systolic function before and after MSC transplantation.
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China.
authors.forename: Shuangquan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China.
authors.forename: Hairu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ren
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China. jwtian2004@163.com lengxiaoping@hotmail.com.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Leng
mesh_terms: D000818:Animals; D015150:Echocardiography, Doppler; D006352:Heart Ventricles / Q000000981:diagnostic imaging / Q000503:physiopathology; D045164:Mesenchymal Stem Cell Transplantation; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000503:physiopathology; D011817:Rabbits; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging* / Q000503:physiopathology
keywords: echocardiography; mesenchymal stem cells; myocardial infarction; quantitative tissue velocity imaging; tissue Doppler echocardiography