title: PP178. Antenatal sFlt1 and PlGF levels at 20-28weeks of gestation for prediction of the occurrence of preeclampsia: A preliminary report.
abstract: BACKGROUND
  Angiogenic and antiangiogenic factors substantially involve in the pathogenesis of preeclampsia. Levels of these biomarkers alter before the occurrence of disease.
  
  
  OBJECTIVE
  To determine the potential of using antenatal serum levels of soluble fms-like tyrosine kinase (sFlt1) and placental growth factor (PlGF) at 20-28weeks of gestation for prediction of subsequent preeclampsia in non-selected population.
  
  
  METHODS
  A reference range of serum sFlt1, PlGF level and sFlt1/PlGF ratio at 10-42weeks of gestation was determined from 291 healthy pregnant women using automated assays. Through antenatal visits at 20-28weeks of gestation, serum of 1,689 women who had singleton pregnancy were obtained and frozen at -80°C. Serum of the mothers who subsequently develop preeclampsia were thawed and analyzed for sFlt1 and PlGF levels.
  
  
  RESULTS
  At 48 hours postpartum, 21 of 1,689 mothers developed preeclampsia. Serum levels (mean±SEM) of sFlt1, PlGF and sFlt1/PlGF ratio obtained at 20-23(+6)weeks of gestation in preeclampsia (N=8) Vs. normal values were 1,845±697 Vs. 1807±95pg/ml, 339±86 Vs. 542±26pg/ml and 33.9±29.5 Vs. 4.1±0.3 (P = 0.304,0.051 and 0.561) respectively. Serum levels of sFlt1, PlGF and sFlt1/PlGF ratio obtained at 24-27(+6)weeks of gestation in preeclampsia (N=13) Vs. normal values were 2,732±637 Vs. 1576±82pg/ml, 449±57 Vs. 718±52pg/ml and 8.9±3.4 Vs. 2.6±0.2 (P = 0.081,0.006 and 0.017) respectively.
  
  
  CONCLUSIONS
  In non-selected population, automated assay of sFlt1 and PlGF levels and sFlt1/PlGF ratio during 20-28weeks of gestation appear to have potential in prediction of the occurrence of preeclampsia.
authors.affiliation: Maternal-fetal Medicine Unit, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Jantarasaengaram
authors.affiliation: Maternal-fetal Medicine Unit, Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Tansatit
authors.affiliation: Dept. of Pathology, Rajavithi Hospital, College of Medicine, Rangsit University, Bangkok, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Samkhoompim