title: Denver Papillae Protocol for Objective Analysis of Fungiform Papillae.
abstract: The goal of the Denver Papillae Protocol is to use a dichotomous key to define and prioritize the characteristics of fungiform papillae (FP) to ensure consistent scoring between scorers. This protocol builds off of a need that has arisen from the last two decades of taste research using FP as a proxy for taste pore density. FP density has historically been analyzed using Miller & Reedy's 1990 characterizations of their morphology: round, stained lighter, large, and elevated. In this work, the authors forewarned that stricter definitions of FP morphology needed to be outlined. Despite this call to action, follow up literature has been scarce, with most studies continuing to cite Miller & Reedy's original work. Consequently, FP density reports have been highly variable and, combined with small sample sizes, may contribute to the discrepant conclusions on the role of FP in taste sensitivity. The Genetics of Taste Lab explored this apparent inconsistency in counting and found that scorers were individually prioritizing the importance of these characteristics differently and had no guidance for when a papilla had some, but not all, of the reported qualities of FP. The result of this subjectivity is highly variable FP counts of the same tongue image. The Denver Papillae Protocol has been developed to remedy this consequence through use of a dichotomous key that further defines and prioritizes the importance of the characteristics put forth by Miller & Reedy. The proposed method could help create a standard way to quantify FP for researchers in the field of taste and nutritional studies.
authors.affiliation: Health Sciences Department, Denver Museum of Nature & Science; tnuessle@dmns.org.
authors.forename: Tiffany M
authors.initials: TM
authors.lastname: Nuessle
authors.affiliation: Health Sciences Department, Denver Museum of Nature & Science.
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Garneau
authors.affiliation: Health Sciences Department, Denver Museum of Nature & Science.
authors.forename: Meghan M
authors.initials: MM
authors.lastname: Sloan
authors.affiliation: Department of Mathematical and Statistical Sciences, University of Colorado Denver.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Santorico
mesh_terms: D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008297:Male; D010781:Photography / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D013650:Taste Buds / Q000033:anatomy & histology* / Q000166:cytology; D014059:Tongue / Q000033:anatomy & histology* / Q000166:cytology