title: Decreased microRNA-143 expression and its tumor suppressive function in human oral squamous cell carcinoma.
abstract: MicroRNA-143 serves as a tumor suppressor in many human malignancies. However, its involvement in oral squamous cell carcinoma (OSCC) is still unclear. In this study, we investigated the effects of miR-143 in OSCC tumorigenesis and development. Using real-time quantitative reverse transcription-polymerase chain reaction, we detected miR-143 expression in 109 pairs of human OSCC and adjacent noncancerous tissues. The associations between miR-143 expression and clinicopathological factors and prognosis of OSCC patients were also statistically analyzed. Further, the effects of miR-143 on the biological behavior of OSCC cells were investigated. miR-143 expression was significantly downregulated in OSCC tissue samples and cell lines. Decreased miR-143 expression was significantly associated with advanced T classifications, positive N classification, advanced TNM stage, and shorter overall survival. In addition, upregulation of miR-143 in Tca8113 cells reduced cell proliferation, invasion, and migration, as well as promoted cell apoptosis in vitro. These findings validate the clinical significance of miR-143 in OSCC and reveal that it may be an intrinsic regulator of tumor progression and a potential prognostic factor for this disease.
authors.affiliation: Department of Orthodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Ni
authors.affiliation: Department of Orthodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: F O
authors.initials: FO
authors.lastname: Lin
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University Wenzhou, China.
authors.forename: D F
authors.initials: DF
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D016019:Survival Analysis
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs