title: ESEM Detection of Foreign Metallic Particles inside Ameloblastomatous Cells.
abstract: Ameloblastoma is a borderline tumor of odontogenic origin, with a high recurrence rate and possible local aggressiveness. The etiopathogenetic factors involved in its occurrence are not still defined and our study has been precisely aimed to search for novel factors associated with its development. Sections cut from paraffin blocks, containing the representative specimens of 18 different ameloblastomas, collected in a 15-year period (1999-2014), have been observed by an environmental scanning electron microscope, in order to search micro- and nano-sized particles and to identify their composition. In all the neoplastic cases, micro- and nano-sized metallic debris, differing in size and composition, have been detected inside the ameloblastomatous cells. On the contrary, the total absence of metallic particles in the healthy control cases has been emerged. Our results reveal a relationship between ameloblastoma and metallic particulate. The cigarette smoke and the routine dental practice appear the most probable source for the presence of these biopersistant inorganic particles inside the neoplastic cells.
authors.affiliation: a Department of Diagnostic and Clinical Medicine and of Public Health , Section of Pathology, University of Modena and Reggio Emilia , Modena (MO) , Italy .
authors.forename: Luca
authors.initials: L
authors.lastname: Roncati
authors.affiliation: b National Research Council, Institute of Science and Technology for Ceramics , Faenza (RA) , Italy .|c Nanodiagnostics Laboratory , San Vito di Spilamberto , Modena (MO) , Italy , and.
authors.forename: Antonietta Morena
authors.initials: AM
authors.lastname: Gatti
authors.affiliation: d Provincial Health Care Services, Institute of Pathology, Santa Maria del Carmine Hospital , Rovereto (TN) , Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Pusiol
authors.affiliation: a Department of Diagnostic and Clinical Medicine and of Public Health , Section of Pathology, University of Modena and Reggio Emilia , Modena (MO) , Italy .
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Barbolini
authors.affiliation: a Department of Diagnostic and Clinical Medicine and of Public Health , Section of Pathology, University of Modena and Reggio Emilia , Modena (MO) , Italy .
authors.forename: Antonio
authors.initials: A
authors.lastname: Maiorana
authors.affiliation: c Nanodiagnostics Laboratory , San Vito di Spilamberto , Modena (MO) , Italy , and.
authors.forename: Stefano
authors.initials: S
authors.lastname: Montanari
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000564:Ameloblastoma / Q000737:chemistry / Q000648:ultrastructure*; D005260:Female; D005547:Foreign Bodies; D006801:Humans; D007573:Jaw Neoplasms / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D008670:Metals / Q000032:analysis*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D008670:Metals
keywords: Ameloblastoma; cigarette smoke; dental practice; environmental scanning electron microscope (ESEM); heavy metals; metallic particles