title: Influence of PAHs among other coastal environmental variables on total and PAH-degrading bacterial communities.
abstract: We evaluated the relative impact of anthropogenic polycyclic aromatic hydrocarbons (PAHs) among biogeochemical variables on total, metabolically active, and PAH bacterial communities in summer and winter in surface microlayer (SML) and subsurface seawaters (SSW) across short transects along the NW Mediterranean coast from three harbors, one wastewater effluent, and one nearshore observatory reference site. At both seasons, significant correlations were found between dissolved total PAH concentrations and PAH-degrading bacteria that formed a gradient from the shore to nearshore waters. Accumulation of PAH degraders was particularly high in the SML, where PAHs accumulated. Harbors and wastewater outfalls influenced drastically and in a different way the total and active bacterial community structure, but they only impacted the communities from the nearshore zone (<2 km from the shore). By using direct multivariate statistical analysis, we confirmed the significant effect of PAH concentrations on the spatial and temporal dynamic of total and active communities in this area, but this effect was putted in perspective by the importance of other biogeochemical variables.
authors.affiliation: UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Sorbonne Universités, F-66650, Banyuls/mer, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Sauret
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Tedetti
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Guigue
authors.affiliation: UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Sorbonne Universités, F-66650, Banyuls/mer, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Dumas
authors.affiliation: UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Sorbonne Universités, F-66650, Banyuls/mer, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Lami
authors.affiliation: UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Sorbonne Universités, F-66650, Banyuls/mer, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Pujo-Pay
authors.affiliation: UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Sorbonne Universités, F-66650, Banyuls/mer, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Conan
authors.affiliation: Aix Marseille Université, CNRS, Université de Toulon, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Goutx
authors.affiliation: UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, Sorbonne Universités, F-66650, Banyuls/mer, France. ghiglione@obs-banyuls.fr.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Ghiglione
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D008522:Mediterranean Sea; D059013:Microbial Consortia / Q000187:drug effects* / Q000235:genetics; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012621:Seasons; D012623:Seawater / Q000737:chemistry / Q000382:microbiology; D012995:Solubility; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: CE-SSCP; MPN; Marine microbial ecotoxicology; PAH; Sea surface microlayer; qPCR