title: Hi-C in Budding Yeast.
abstract: Hi-C enables simultaneous detection of interaction frequencies between all possible pairs of restriction fragments in the genome. The Hi-C method is based on chromosome conformation capture (3C), which uses formaldehyde cross-linking to fix chromatin regions that interact in three-dimensional space, irrespective of their genomic locations. In the Hi-C protocol described here, cross-linked chromatin is digested with HindIII and the ends are filled in with a nucleotide mix containing biotinylated dCTP. These fragments are ligated together, and the resulting chimeric molecules are purified and sheared to reduce length. Finally, biotinylated ligation junctions are pulled down with streptavidin-coated beads, linked to high-throughput sequencing adaptors, and amplified via polymerase chain reaction (PCR). The resolution of the Hi-C data set will depend on the depth of sequencing and choice of restriction enzyme. When sufficient sequence reads are obtained, information on chromatin interactions and chromosome conformation can be derived at single restriction fragment resolution for complete genomes.
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Jon-Matthew
authors.initials: JM
authors.lastname: Belton
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Job
authors.initials: J
authors.lastname: Dekker
mesh_terms: D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D015825:Chromosomes, Fungal / Q000737:chemistry / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D004718:Saccharomycetales / Q000235:genetics*
chemical_list: D002843:Chromatin