title: Genetic profiling of yeast industrial strains using in situ comparative genomic hybridization (CGH).
abstract: The genetic differences and changes in genomic stability may affect fermentation processes involving baker's, brewer's and wine yeast strains. Thus, it seems worthwhile to monitor the changes in genomic DNA copy number of industrial strains. In the present study, we developed an in situ comparative genomic hybridization (CGH) to investigate the ploidy and genetic differences between selected industrial yeast strains. The CGH-based system was validated using the laboratory Saccharomyces cerevisiae yeast strains (haploid BY4741 and diploid BY4743). DNA isolated from BY4743 cells was considered a reference DNA. The ploidy and DNA gains and losses of baker's, brewer's and wine strains were revealed. Taken together, the in situ CGH was shown a helpful molecular tool to identify genomic differences between yeast industrial strains. Moreover, the in situ CGH-based system may be used at the single-cell level of analysis to supplement array-based techniques and high-throughput analyses at the population scale.
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, PL 35-959 Rzeszow, Poland. Electronic address: mawnuk@gmail.com.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, PL 35-959 Rzeszow, Poland.
authors.forename: Anita
authors.initials: A
authors.lastname: Panek
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, PL 35-959 Rzeszow, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Golec
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, PL 35-959 Rzeszow, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Magda
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, PL 35-959 Rzeszow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Deregowska
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, PL 35-959 Rzeszow, Poland.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Adamczyk
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
mesh_terms: D055028:Comparative Genomic Hybridization / Q000379:methods*; D056915:DNA Copy Number Variations; D005285:Fermentation; D016681:Genome, Fungal; D023281:Genomics / Q000379:methods*; D007218:Industrial Microbiology; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics*; D059010:Single-Cell Analysis
keywords: Genomic instability; In situ comparative genomic hybridization; Industrial yeast