title: SCARECROW-LIKE15 interacts with HISTONE DEACETYLASE19 and is essential for repressing the seed maturation programme.
abstract: Epigenetic regulation of gene expression is critical for controlling embryonic properties during the embryo-to-seedling phase transition. Here we report that a histone deacetylase19 (HDA19)-associated regulator, scarecrow-like15 (SCL15), is essential for repressing the seed maturation programme in vegetative tissues. SCL15 is expressed in and GFP-tagged SCL15 predominantly localizes to, the vascular bundles particularly in the phloem companion cells and neighbouring specialized cells. Mutation of SCL15 leads to a global shift in gene expression in seedlings to a profile resembling late embryogenesis in seeds. In scl15 seedlings, many genes involved in seed maturation are markedly derepressed with concomitant accumulation of seed 12S globulin; this is correlated with elevated levels of histone acetylation at a subset of seed-specific loci. SCL15 physically interacts with HDA19 and direct targets of HDA19-SCL15 association are identified. These studies reveal that SCL15 acts as an HDA19-associated regulator to repress embryonic traits in seedlings.
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Gao
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Gordon M
authors.initials: GM
authors.lastname: Gropp
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Branimir
authors.initials: B
authors.lastname: Gjetvaj
authors.affiliation: Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5.
authors.forename: Donna L
authors.initials: DL
authors.lastname: Lindsay
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wei
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Cathy
authors.initials: C
authors.lastname: Coutu
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Wan
authors.affiliation: Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, Canada N5V 4T3.
authors.forename: Abdelali
authors.initials: A
authors.lastname: Hannoufa
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Lydiate
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Margaret Y
authors.initials: MY
authors.lastname: Gruber
authors.affiliation: Institute for Cellular and Molecular Biology, Center for Computational Biology and Bioinformatics, University of Texas at Austin, Austin, Texas 78712, USA.
authors.forename: Z Jeffrey
authors.initials: ZJ
authors.lastname: Chen
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, Canada S7N 0X2.
authors.forename: Dwayne D
authors.initials: DD
authors.lastname: Hegedus
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006655:Histone Deacetylases / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D058526:Plant Vascular Bundle / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*; D012639:Seeds / Q000254:growth & development*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D012097:Repressor Proteins; C000606764:SCARECROW-LIKE15 protein, Arabidopsis; C507152:HDA19 protein, Arabidopsis; D006655:Histone Deacetylases