title: Nano-hydroxyapatite/chitosan-starch nanocomposite as a novel bone construct: Synthesis and in vitro studies.
abstract: A novel ternary nanocomposite system incorporating hydroxyapatite, chitosan and starch (n-HA/CS-ST) has been synthesized by co-precipitation method at room temperature, addressing the issues of biocompatibility, mechanical strength and cytotoxicity required for bone tissue engineering. The interactions, crystallite size, surface morphology and thermal stability against n-HA/CS nanocomposite have been obtained by comparing the results of FTIR, SEM, TEM, DLS, XRD and TGA/DTA. A comparative study of bioactivity and thermal stability of n-HA/CS and n-HA/CS-ST nanocomposites revealed that the incorporation of starch as templating agent enhanced these properties in n-HA/CS-ST nanocomposite. A lower swelling rate of n-HA/CS-ST relative to n-HA/CS indicates a higher mechanical strength supportive of bone tissue ingrowths. The MTT assay on murine fibroblast L929 and human osteoblasts-like MG-63 cells and in vitro bioactivity of n-HA/CS-ST matrix referred superior non-toxic nature of n-HA/CS-ST nanocomposite and greater possibility of osteointegration in vivo respectively. Furthermore n-HA/CS-ST exhibited improved antibacterial property against both Gram-positive and Gram-negative bacteria relative to n-HA/CS.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India. Electronic address: shakir078@yahoo.co.in.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shakir
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Reshma
authors.initials: R
authors.lastname: Jolly
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Mohd Shoeb
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Noor e
authors.initials: Ne
authors.lastname: Iram
authors.affiliation: Department of Microbiology, J.N. Medical College, A.M.U., Aligarh, India.
authors.forename: Haris M
authors.initials: HM
authors.lastname: Khan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D032701:Biomimetics; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002460:Cell Line; D060326:Chemistry Techniques, Synthetic; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D055595:Mechanical Phenomena; D051379:Mice; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013213:Starch / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D013213:Starch; D048271:Chitosan; D017886:Durapatite
keywords: Biomaterial; Chitosan; Hydroxyapatite; In vitro bioactivity; Starch