title: Effect of BMP7 on podocyte transdifferentiation and Smad7 expression induced by hyperglycemia.
abstract: OBJECTIVE
  To investigate the effect of BMP7 on the transdifferentiation and Smad7 expression of podocytes induced by high glucose in vitro and to explore its possible protective mechanisms.
  
  
  METHODS
  Mouse podocytes were cultured and divided into normal glucose group (NG), high glucose group (HG), mannitol group, NG+BMP7 group, and HG+BMP7 group. Real-time PCR and Western blot were applied respectively to detect the mRNA and protein expression levels of synaptopodin, desmin, and Smad7.
  
  
  RESULTS
  The cells significantly up-regulated the mRNA and protein expression of desmin and reduced the expression of both synaptopodin and Smad7 after 48 hours (vs. NG, p < 0.01). BMP7 dramatically suppressed the mRNA and protein expression of desmin and protected the expression of synaptopodin and Smad7 after incubation with high glucose for 48 hours (vs. HG, p < 0.01).
  
  
  CONCLUSIONS
  BMP7 can inhibit the epithelial-to-mesenchymal cell transformation (EMT) of podocytes induced by high glucose; Smad7 may mediate the blunting effects of BMP7 on high glucose in podocytes.
authors.forename: Liqiu
authors.initials: L
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Fu
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Leping
authors.initials: L
authors.lastname: Shao
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects; D003893:Desmin / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000503:physiopathology*; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D008840:Microfilament Proteins / Q000187:drug effects; D050199:Podocytes / Q000187:drug effects*; D051906:Smad7 Protein / Q000187:drug effects*; D013997:Time Factors
chemical_list: D055419:Bone Morphogenetic Protein 7; D003893:Desmin; D008840:Microfilament Proteins; D051906:Smad7 Protein; C497807:Smad7 protein, mouse; C503968:Synpo protein, mouse; C525820:bmp7 protein, mouse; D008353:Mannitol; D005947:Glucose