title: PP005. Vitamin D depletion aggravates hypertension in transgenic rats.
abstract: BACKGROUND
  Vitamin D may ameliorate hypertension and kidney disease through genomic and extra-genomic pathways.
  
  
  OBJECTIVE
  To investigate the impact of vitamin D in a transgenic rat model of angiotensin II-mediated hypertensive organ failure.
  
  
  METHODS
  In 4-week-old age-matched rats overexpressing the human renin and angiotensinogen genes, group 1 (n=18) received vitamin D depleted chow; group 2 (n=15) standard chow and intraperitoneal paricalcitol at 800ng/kg thrice weekly; and group 3 (n=15) standard chow and vehicle injections. Blood pressure (tail cuff) and 24-h albuminuria were determined once weekly. After three weeks, animals were sacrificed. Heart tissue was examined for atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) by RT-PCR.
  
  
  RESULTS
  The vitamin D depleted group had higher blood pressure at week 1 (mean difference 23.4mmHg, 95% CI 9.1-37.7) and tended to have higher blood pressure in weeks 2 and 3 (mean difference 14.3mmHg 95% CI -0.02-28.7 and 15.2mmHg 95% CI -1.5-33). The depletion group had higher heart-to-body weight ratio, and a trend towards higher ANP and BNP levels. The group receiving paricalcitol did not perform better. No differences were found between groups in mortality or proteinuria.
  
  
  CONCLUSIONS
  Short-term vitamin D depletion aggravated hypertension and end-organ damage in a rat model of angiotensin II-induced hypertension. Short-term interventions with high-dose vitamin D analogues had no protective effect.
authors.forename: Louise
authors.initials: L
authors.lastname: Bjørkholt Andersen
authors.forename: Florian
authors.initials: F
authors.lastname: Herse
authors.forename: Henrik Thybo
authors.initials: HT
authors.lastname: Christesen
authors.forename: Ralf
authors.initials: R
authors.lastname: Dechend
authors.forename: Dominik
authors.initials: D
authors.lastname: Müller