title: RAD51 G135C genetic polymorphism and their potential role in gastric cancer induced by Helicobacter pylori infection in Bhutan.
abstract: In order to evaluate the role of the RAD51 G135C genetic polymorphism on the risk of gastric cancer induced by Helicobacter pylori infection, we determined allele frequency and genotype distribution of this polymorphism in Bhutan--a population documented with high prevalence of gastric cancer and extremely high prevalence of H. pylori infection. The status of RAD51 G135C was examined by restriction fragment length polymorphism analysis of PCR amplified fragments and sequencing. Histological scores were evaluated according to the updated Sydney system. G135C carriers showed significantly higher scores for intestinal metaplasia in the antrum than G135G carriers [mean (median) 0·33 (0) vs. 0·08 (0), P = 0·008]. Higher scores for intestinal metaplasia of G135C carriers compared to those of G135G carriers were also observed in H. pylori-positive patients [0·3 (0) vs. 0·1 (0), P = 0·002] and H. pylori-positive patients with gastritis [0·4 (0) vs. 0·1 (0), P = 0·002] but were not found in H. pylori-negative patients. Our findings revealed that a combination of H. pylori infection and RAD51 G135C genotype of the host showed an increasing score for intestinal metaplasia. Therefore, RAD51 G135C might be the important predictor for gastric cancer of H. pylori-infected patients.
authors.affiliation: Department of Environmental and Preventive Medicine,Oita University Faculty of Medicine,Yufu City,Oita,Japan.
authors.forename: T T H
authors.initials: TT
authors.lastname: Trang
authors.affiliation: Department of Environmental and Preventive Medicine,Oita University Faculty of Medicine,Yufu City,Oita,Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagashima
authors.affiliation: Department of Molecular Pathology,Oita University Faculty of Medicine,Yufu,Japan.
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.affiliation: Gastroenterology Unit,Department of Medicine,Thammas at University Hospital,Pathumthani,Thailand.
authors.forename: V
authors.initials: V
authors.lastname: Mahachai
authors.affiliation: Department of Gastroenterology,Bangkok Hospital,Bangkok,Thailand.
authors.forename: R-K
authors.initials: RK
authors.lastname: Vilaichone
authors.affiliation: Department of Surgery,Jigme Dorji Wangchuk National Referral Hospital,Thimphu,Bhutan.
authors.forename: L
authors.initials: L
authors.lastname: Tshering
authors.affiliation: Department of Endoscopy,Cho Ray Hospital,Ho Chi Minh,Vietnam.
authors.forename: T T
authors.initials: TT
authors.lastname: Binh
authors.affiliation: Department of Environmental and Preventive Medicine,Oita University Faculty of Medicine,Yufu City,Oita,Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaoka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001631:Bhutan / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008679:Metaplasia / Q000453:epidemiology* / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011706:Pyloric Antrum / Q000473:pathology; D051135:Rad51 Recombinase / Q000235:genetics* / Q000378:metabolism; D018570:Risk Assessment; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics / Q000382:microbiology; D055815:Young Adult
chemical_list: C495153:RAD51 protein, human; D051135:Rad51 Recombinase
keywords: Gastric cancer; Helicobacter pylori infection; RAD51; polymorphisms