title: [Infectivity of promastigotes in stationary phase of Leishmania (viannia) braziliensis and leishmania (viannia) peruviana, In cell line dh82].
abstract: OBJECTIVE
  To determine the infectivity of promastigotes of Leishmania (V.) peruviana and Leishmania (V.) braziliensis in monocyte-macrophage cell line DH82 of Canis familiaris.
  
  
  METHODS
  Was conducted a experimental study during the months of january to december 2013. Were used strains of Leishmania were used (V.) braziliensis MHOM/PE/84/LC53 and Leishmania (V.) peruviana MHOM/PE/84/LC26. The cell line was infected with stationary phase promastigotes and infectivity was determined as the product of percent infected macrophages average amastigotes per macrophage observed in epifluorescence microscope.
  
  
  RESULTS
  13% of metacyclic forms to Leishmania (V.) braziliensis corresponded to 17.5 days post inoculation and Leishmania (V.) peruviana a percentage of 9.5% on the day 14.5. No significant difference was found between infectivity of stationary phase promastigotes of both species.
  
  
  CONCLUSIONS
  It is recommended assess the infectivity of metacyclic promastigotes peruviana strains of Leishmania (V.) and Leishmania (V.) braziliensis cell lines in order to determine the most appropriate model in vitro infection, allowing leishmanicidas make the drug more effective susceptibility studies for disease control.
authors.affiliation: Universidad Nacional de Trujillo, Trujillo, Perú
authors.forename: Karen Daphne
authors.initials: KD
authors.lastname: Calvay-Sánchez
authors.affiliation: Instituto Nacional de Salud, Lima, Perú
authors.forename: Nyshon Máximo
authors.initials: NM
authors.lastname: Rojas-Palomino
authors.affiliation: Instituto Nacional de Salud, Lima, Perú
authors.forename: Aidé Clorinda
authors.initials: AC
authors.lastname: Sandoval-Juarez
authors.affiliation: Instituto Nacional de Salud, Lima, Perú
authors.forename: Alberto
authors.initials: A
authors.lastname: Cisneros-Tarmeño
authors.affiliation: Unidad de Análisis y Generación de Evidencias en Salud Pública, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú
authors.forename: Cristian
authors.initials: C
authors.lastname: Obregón-Cahuaya
authors.affiliation: Instituto Nacional de Salud, Lima, Perú
authors.forename: Gloria Sonia
authors.initials: GS
authors.lastname: Minaya-Gómez
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D007892:Leishmania braziliensis / Q000254:growth & development / Q000472:pathogenicity*