title: Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour.
abstract: A highly potent secondary metabolite producing endophytic strain, Streptomyces sp. HUST012 was isolated from the stems of the medicinal plant Dracaena cochinchinensis Lour. Strain HUST012 showed antimicrobial and antitumor activities which were significantly much higher than those of dragon's blood extracted from D. cochinchinensis Lour. On further analysis, the strain was found to produce two metabolites, SPE-B11.8 (elucidated to be a novel metabolite (Z)-tridec-7-ene-1,2,13-tricarboxylic acid) and SPE-B5.4 (elucidated as Actinomycin-D). The Minimum Inhibitory Concentration values of SPE-B11.8 against a set of test bacterial organisms (Methicillin-resistant Staphylococcus epidermis ATCC 35984, Methicillin-resistant Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 13883) ranged between 15.63 and 62.5 μg/ml while that for SPE-B5.4 ranged between 0.04 and 2.24 μg/ml. The compound SPE-B11.8 showed cytotoxic effect at 41.63 and 29.54 μg/ml IC 50-values against Hep G2 and MCF-7, respectively, while the compound SPE-B5.4 exhibited stronger activities against them at 0.23 and 0.18 μg/ml IC 50-values.
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University Kunming, China ; Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology Hanoi, Vietnam.
authors.forename: Thi-Nhan
authors.initials: TN
authors.lastname: Khieu
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University Kunming, China ; Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University Kunming, China.
authors.forename: Min-Jiao
authors.initials: MJ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University Guangzhou, China.
authors.forename: Salam
authors.initials: S
authors.lastname: Nimaichand
authors.affiliation: Laboratory of Fermentation Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology Hanoi, Vietnam.
authors.forename: Ngoc-Tung
authors.initials: NT
authors.lastname: Quach
authors.affiliation: Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology Hanoi, Vietnam.
authors.forename: Son
authors.initials: S
authors.lastname: Chu-Ky
authors.affiliation: Laboratory of Fermentation Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology Hanoi, Vietnam.
authors.forename: Quyet-Tien
authors.initials: QT
authors.lastname: Phi
authors.affiliation: Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology Hanoi, Vietnam.
authors.forename: Thu-Trang
authors.initials: TT
authors.lastname: Vu
authors.affiliation: Department of Bioactive Products, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology Hanoi, Vietnam.
authors.forename: Tien-Dat
authors.initials: TD
authors.lastname: Nguyen
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University Kunming, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University Kunming, China.
authors.forename: Deene M
authors.initials: DM
authors.lastname: Prabhu
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University Kunming, China ; State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University Guangzhou, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
keywords: (Z)-tridec-7-ene-1,2,13-tricarboxylic acid; Actinomycin-D; Dracaena cochinchinensis Lour.; Streptomyces sp. HUST012; antimicrobial and cytotoxic activities; endophytic