title: Labeling Cell Surface GPIs and GPI-Anchored Proteins through Metabolic Engineering with Artificial Inositol Derivatives.
abstract: Glycosylphosphatidylinositol (GPI) anchoring of proteins to the cell surface is important for various biological processes, but GPI-anchored proteins are difficult to study. An effective strategy was developed for the metabolic engineering of cell-surface GPIs and GPI-anchored proteins by using inositol derivatives carrying an azido group. The azide-labeled GPIs and GPI-anchored proteins were then tagged with biotin on live cells through a click reaction, which allows further elaboration with streptavidin-conjugated dyes or other molecules. The strategy can be used to label GPI-anchored proteins with various tags for biological studies.
authors.affiliation: National Glycoengineering Research Center, Shandong University, 29 Shanda Nan Lu, Jinan 250010 (China).
authors.forename: Lili
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202 (the United States).
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: National Glycoengineering Research Center, Shandong University, 29 Shanda Nan Lu, Jinan 250010 (China).
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Guo
mesh_terms: D001386:Azides / Q000737:chemistry* / Q000378:metabolism; D019921:Biotinylation; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D057930:Click Chemistry; D058851:GPI-Linked Proteins / Q000032:analysis* / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000031:analogs & derivatives* / Q000378:metabolism; D060847:Metabolic Engineering; D008853:Microscopy; D013194:Staining and Labeling
chemical_list: D001386:Azides; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D007294:Inositol
keywords: GPI anchor; carbohydrates; glycolipids; inositol; metabolic engineering