title: Prognostic Value of a CYP2B6 Gene Polymorphism in Patients with Acute Myeloid Leukemia.
abstract: BACKGROUND
  The objectives of this study aimed to detect a CYP2B6 polymorphism in de novo cases of acute myeloid leukemia patients and identify any role in disease progression and outcome.
  
  
  METHODS
  DNA was isolated from peripheral blood of 82 newly diagnosed acute myeloid leukemia cases and the CYP2B6 G15631T gene polymorphism was assayed by PCR restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The frequency of the GG genotype (wild type) was 48 (58.5%) and that of the mutant type T allele was 34 (41.9%). GT genotype heterozygous variants were found in 28 (34%), and TT genotype homozygous variants in 6 (7.3%) cases. We found no significant association between the CYP2B6 G15631T polymorphism and complete response (CR) (p-value=0.768), FAB classification (p-value=0.51), cytogenetic analysis (p-value=0.673), and overall survival (p-value=0.325). Also, there were no significant links with early toxic death (p-value=0.92) or progression- free survival (PFS) (p-value=0.245).
  
  
  CONCLUSIONS
  Our results suggest that the CYP2B6 polymorphism has no role in disease progression, therapeutic outcome, patient free survival, early toxic death and overall survival in acute myeloid leukemia patients.
authors.affiliation: Department of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt E-mail : nevin_elazhary@hotmail.com.
authors.forename: Nevin M
authors.initials: NM
authors.lastname: Alazhary
authors.forename: Roxan E
authors.initials: RE
authors.lastname: Shafik
authors.forename: Hanan E
authors.initials: HE
authors.lastname: Shafik
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Kamel
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D065702:Cytochrome P-450 CYP2B6 / Q000235:genetics*; D020732:Cytogenetic Analysis; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012074:Remission Induction; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D065702:Cytochrome P-450 CYP2B6