title: Ultrasonic Nanobubbles Carrying Anti-PSMA Nanobody: Construction and Application in Prostate Cancer-Targeted Imaging.
abstract: To facilitate prostate cancer imaging using targeted molecules, we constructed ultrasonic nanobubbles coupled with specific anti-PSMA (prostate specific membrane antigen) nanobodies, and evaluated their in vitro binding capacity and in vivo imaging efficacy. The "targeted" nanobubbles, which were constructed via a biotin-streptavidin system, had an average diameter of 487.60 ± 33.55 nm and carried the anti-PSMA nanobody as demonstrated by immunofluorescence. Microscopy revealed targeted binding of nanobubbles in vitro to PSMA-positive cells. Additionally, ultrasonography indicators of nanobubble imaging (including arrival time, peak time, peak intensity and enhanced duration) were evaluated for the ultrasound imaging in three kinds of animal xenografts (LNCaP, C4-2 and MKN45), and showed that these four indicators of targeted nanobubbles exhibited significant differences from blank nanobubbles. Therefore, this study not only presents a novel approach to target prostate cancer ultrasonography, but also provides the basis and methods for constructing small-sized and high-efficient targeted ultrasound nanobubbles.
authors.affiliation: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Urology, Daping Hospital, Institute of Surgery Research, Third Military Medical University, Chongqing, China.
authors.forename: Luofu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: Zhui
authors.initials: Z
authors.lastname: Tu
authors.affiliation: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang City, Jiangxi Province, China; Jiangxi-QAI Joint Research Institute, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Kejing
authors.initials: K
authors.lastname: Fang
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D045744:Cell Line, Tumor; D043425:Glutamate Carboxypeptidase II / Q000037:antagonists & inhibitors* / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D061905:Single-Domain Antibodies / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D061905:Single-Domain Antibodies; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II