title: Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL.
abstract: Three novel QTL for peroxidase activity were mapped, and gene-specific markers for TaPod-A1 were developed and validated using RILs derived from the Doumai/Shi 4185 cross and 281 wheat cultivars. TaPod-A1 is within one of the three QTL. Peroxidase (POD) activity in grain is an important factor determining the color of flour and end-use products of wheat, such as noodles and steamed bread. Mapping QTL for POD activity, characterization of POD genes and development of gene-specific markers are important for molecular marker-assisted selection in wheat breeding. Quantitative trait loci (QTL) for POD activity in common wheat were mapped using a recombinant inbred line (RIL) population derived from a Doumai/Shi 4185 cross grown in four environments and genotyped using the wheat 90 K iSelect assay. Three novel QTL for POD activity, QPod.caas-3AL, QPod.caas-4BS and QPod.caas-5AS, were identified on chromosomes 3AL, 4BS and 5AS, explaining 5.3-21.2% of phenotypic variance across environments. The full-length genomic DNA (gDNA) sequence of a POD gene, designated TaPod-A1, on chromosome 3A was characterized by homolog cloning and PCR verification. Two complementary dominant sequence-tagged site (STS) markers, POD-3A1 and POD-3A2, were developed based on single nucleotide polymorphisms (SNPs) between two alleles at the TaPod-A1 locus, amplifying 291- and 766-bp fragments in cultivars with lower and higher POD activities, respectively. The two gene-specific markers were mapped on chromosome 3AL using a set of Chinese Spring (CS) nulli-tetrasomic lines, and ditelosomic lines 3AL and 3AS. QTL analysis indicated that QPod.caas-3AL co-segregated with the gene-specific markers POD-3A1 and POD-3A2. POD-3A1 and POD-3A2 were verified on 281 wheat cultivars and advanced lines, and showed significant (P < 0.05) associations with POD activities. POD-3A1 and POD-3A2 may be useful as markers for improving color attributes in wheat breeding programs.
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Wei
authors.affiliation: College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Geng
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
authors.forename: Weie
authors.initials: W
authors.lastname: Wen
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. xiaxianchun@caas.cn.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Xia
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. chenxinmin@caas.cn.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Chen
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. zhhecaas@163.com.|International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China. zhhecaas@163.com.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: He
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D010544:Peroxidases / Q000235:genetics*; D010641:Phenotype; D000069600:Plant Breeding; D040641:Quantitative Trait Loci; D016324:Sequence Tagged Sites; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D010544:Peroxidases