title: Trypanocidal, trichomonacidal and cytotoxic components of cultivated Artemisia absinthium Linnaeus (Asteraceae) essential oil.
abstract: Artemisia absinthium is an aromatic and medicinal plant of ethnopharmacological interest and it has been widely studied. The use ofA. absinthium based on the collection of wild populations can result in variable compositions of the extracts and essential oils (EOs). The aim of this paper is the identification of the active components of the vapour pressure (VP) EO from a selected and cultivated A. absinthium Spanish population (T2-11) against two parasitic protozoa with different metabolic pathways: Trypanosoma cruzi and Trichomonas vaginalis. VP showed activity on both parasites at the highest concentrations. The chromatographic fractionation of the VP T2-11 resulted in nine fractions (VLC1-9). The chemical composition of the fractions and the antiparasitic effects of fractions and their main compounds suggest that the activity of the VP is related with the presence of trans-caryophyllene and dihydrochamazulene (main components of fractions VLC1 and VLC2 respectively). Additionally, the cytotoxicity of VP and fractions has been tested on several tumour and no tumour human cell lines. Fractions VLC1 and VLC2 were not cytotoxic against the nontumoural cell line HS5, suggesting selective antiparasitic activity for these two fractions. The VP and fractions inhibited the growth of human tumour cell lines in a dose-dependent manner.
authors.affiliation: Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, ES.
authors.forename: Rafael Alberto
authors.initials: RA
authors.lastname: Martínez-Díaz
authors.affiliation: Facultad de Farmacia, Campus de Excelencia Internacional Moncloa, Universidad Complutense de Madrid, Madrid, ES.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ibáñez-Escribano
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria, Madrid, ES.
authors.forename: Jesús
authors.initials: J
authors.lastname: Burillo
authors.affiliation: Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, ES.
authors.forename: Lorena de Las
authors.initials: Lde L
authors.lastname: Heras
authors.affiliation: Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, ES.
authors.forename: Gema Del
authors.initials: GD
authors.lastname: Prado
authors.affiliation: Instituto de Investigación Hospital 12 de Octubre, Madrid, ES.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Agulló-Ortuño
authors.affiliation: Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Madrid, ES.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Julio
authors.affiliation: Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Madrid, ES.
authors.forename: Azucena
authors.initials: A
authors.lastname: González-Coloma
mesh_terms: D031171:Artemisia absinthium / Q000737:chemistry*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014244:Trichomonas / Q000187:drug effects*; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts