title: Response of osteoblasts and preosteoblasts to calcium deficient and Si substituted hydroxyapatites treated at different temperatures.
abstract: Hydroxyapatite (HA) is a calcium phosphate bioceramic widely used for bone grafting and augmentation purposes. The biological response of HA can be improved through chemical and microstructural modifications, as well as by manufacturing it as macroporous implants. In the present study, calcium deficient hydroxyapatite (CDHA) and Si substituted hydroxyapatite (SiHA) macroporous scaffolds have been prepared by robocasting. In order to obtain different microstructural properties, the scaffolds have been treated at 700°C and 1250°C. The scaffolds have been characterized and tested as supports for both osteoblast growth and pre-osteoblast differentiation, as fundamental requisite for their potential use in bone tissue engineering. Morphology, viability, adhesion, proliferation, cell cycle, apoptosis, intracellular content of reactive oxygen species and interleukin-6 production were evaluated after contact of osteoblasts-like cells with CDHA and SiHA materials. An adequate interaction of osteoblasts-like cells and preosteoblasts-like cells with all these scaffolds was observed. However, the higher bone cell proliferation and differentiation on CDHA and SiHA scaffolds treated at 1250°C and the lower adsorption of albumin and fibrinogen on these materials in comparison to those treated at 700°C, suggest a better tissue response to CDHA and SiHA materials treated at high temperature.
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: María Concepción
authors.initials: MC
authors.lastname: Matesanz
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Linares
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Oñaderra
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Feito
authors.affiliation: Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain; Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Martínez-Vázquez
authors.affiliation: Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain; Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Sánchez-Salcedo
authors.affiliation: Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain; Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. Electronic address: arcosd@ucm.es.
authors.forename: Daniel
authors.initials: D
authors.lastname: Arcos
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain. Electronic address: portoles@quim.ucm.es.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Portolés
authors.affiliation: Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain; Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. Electronic address: vallet@ucm.es.
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D012825:Silicon / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D006882:Hydroxyapatites; D002118:Calcium; D012825:Silicon
keywords: Fibrinogen; Hydroxyapatite; Osteoblast; Preosteoblast; Scaffold