title: DNA Assembly Tools and Strategies for the Generation of Plasmids.
abstract: Since the discovery of restriction enzymes and the generation of the first recombinant DNA molecule over 40 years ago, molecular biology has evolved into a multidisciplinary field that has democratized the conversion of a digitized DNA sequence stored in a computer into its biological counterpart, usually as a plasmid, stored in a living cell. In this article, we summarize the most relevant tools that allow the swift assembly of DNA sequences into useful plasmids for biotechnological purposes. We cover the main components and stages in a typical DNA assembly workflow, namely in silico design, de novo gene synthesis, and in vitro and in vivo sequence assembly methodologies.
authors.affiliation: Life Technologies, Carlsbad, CA 92008.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Baek
authors.affiliation: Life Technologies, Carlsbad, CA 92008.
authors.forename: Michael
authors.initials: M
authors.lastname: Liss
authors.affiliation: Life Technologies, Carlsbad, CA 92008.
authors.forename: Kevin
authors.initials: K
authors.lastname: Clancy
authors.affiliation: Life Technologies, Carlsbad, CA 92008.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Chesnut
authors.affiliation: Life Technologies, Carlsbad, CA 92008.
authors.forename: Federico
authors.initials: F
authors.lastname: Katzen
mesh_terms: D001709:Biotechnology / Q000379:methods; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D005813:Genes, Synthetic; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D004247:DNA