title: New approaches for understanding the nuclear force balance in living, adherent cells.
abstract: Cytoskeletal forces are transmitted to the nucleus to position and shape it. Linkages mediated by the LINC (linker of nucleoskeleton and cytoskeleton) complex transfer these forces to the nuclear envelope. Nuclear position and shape can be thought to be determined by a balance of cytoskeletal forces generated by microtubule motors that shear the nuclear surface, actomyosin forces that can pull, push and shear the nucleus, and intermediate filaments that may passively resist nuclear decentering and deformation. Parsing contributions of these different forces to nuclear mechanics is a very challenging task. Here we review new approaches that can be used in living cells to probe and understand the nuclear force balance.
authors.affiliation: Department of Biomedical Engineering, University of Florida, Gainesville FL 32611, United States.
authors.forename: Srujana
authors.initials: S
authors.lastname: Neelam
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville FL 32611, United States.
authors.forename: Richard B
authors.initials: RB
authors.lastname: Dickinson
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville FL 32611, United States. Electronic address: tlele@che.ufl.edu.
authors.forename: Tanmay P
authors.initials: TP
authors.lastname: Lele
mesh_terms: D002448:Cell Adhesion; D002465:Cell Movement; D002467:Cell Nucleus / Q000502:physiology; D003599:Cytoskeleton / Q000502:physiology*; D006801:Humans; D018625:Microscopy, Atomic Force
keywords: Cytoskeleton; LINC complex; Nuclear forces; Nuclear position; Nuclear shape