title: Mitochondrial DNA 4401A>G and 4435A>G Mutations Associated with Essential Hypertension Testing Method.
abstract: BACKGROUND
  Establish a method to identify simultaneously mitochondrial DNA 4401A>G and 4435A>G mutations associated with essential hypertension.
  
  
  METHODS
  The whole genomic DNA of samples carrying mitochondrial DNA 4401A>G and 4435A>G mutations, double mutation (mtDNA 4401A>G and 4435A>G) as well as wild type were used as templates. Specifically amplified mtDNA 234 bp fragments between 4344 - 4577 using nested PCR and digested the PCR purified products simultaneously with two restriction enzymes BfaI and NlaIII. The products were identified by polyacrylamide gel electrophoresis.
  
  
  RESULTS
  Electrophoresis results showed that electrophoresis bands were specific among different samples.
  
  
  CONCLUSIONS
  This study established a convenient, accurate, and suitable for clinical determination of mtDNA 4401 A>G and 4435A>G mutations associated with essential hypertension testing using the new method.
authors.forename: Ling
authors.initials: L
authors.lastname: Xue
authors.forename: Han
authors.initials: H
authors.lastname: Yu
authors.forename: Junwei
authors.initials: J
authors.lastname: Geng
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Tang
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D000075222:Essential Hypertension; D006801:Humans; D006973:Hypertension / Q000175:diagnosis* / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction
chemical_list: D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes