title: Detection of influenza B lineages from 2001 to 2013 in a tertiary hospital in the city of São Paulo, Brazil.
abstract: Two antigenically distinct lineages of influenza B viruses, the Victoria-like and Yamagata-like strains, currently circulate among humans. Surveillance from United States of America and Europe over the last 10 years showed that the chance of a correct matching between vaccine and the circulating lineage had been 50%. We investigated influenza B infection in different patient groups (asymptomatic, general community, with comorbidities and hospitalised) attended at a tertiary hospital in the city of São Paulo, Brazil between 2001-2013. All samples were screened for influenza B virus by one-step real-time reverse transcription-polymerase chain reaction. From 2,992 respiratory samples collected, 114 (3.8%) tested positive for influenza B. Teenagers (13-18 years) presented the highest rate of 18.5% (odds ratio 22.87, 95% confidence interval 2.90-180.66, p < 0.001). One hundred nine samples could be characterised: 50 were Yamagata-like and 59 were Victoria-like strains. Mismatching between the vaccine and predominant circulating strain was observed in 2002 and 2013 seasons. Based on data collected during a period of 12 years, we found that influenza B was more frequent in teenagers. Co-circulation of both lineages and mismatch with the vaccine strain can occur. Our data highlighted the importance of quadrivalent vaccines and future analysis of the age groups included in vaccination programs.
authors.affiliation: Laboratório de Virologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, BR.
authors.forename: Ana Helena
authors.initials: AH
authors.lastname: Perosa
authors.affiliation: Laboratório de Virologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, BR.
authors.forename: Celso
authors.initials: C
authors.lastname: Granato
authors.affiliation: Laboratório de Virologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, BR.
authors.forename: Nancy
authors.initials: N
authors.lastname: Bellei
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009981:Influenza B virus / Q000235:genetics*; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000821:virology*; D010641:Phenotype; D012367:RNA, Viral / Q000235:genetics
chemical_list: D007252:Influenza Vaccines; D012367:RNA, Viral