title: Shikonin inhibits invasiveness of osteosarcoma through MMP13 suppression.
abstract: Osteosarcoma (OS) is the most common primary malignant bone tumor, notorious for its metastasis. We have recently shown that shikonin, an effective constituent extracted from Chinese medicinal herb, induces necroptosis in OS cells. Nevertheless, the effects of low-dose shikonin on the invasiveness of OS cells are unknown. Here, we showed that shikonin dose-dependently decreased OS cell invasiveness in both scratch wound healing assay and transwell cell migration assay. Moreover, the direct target of shikonin on cell invasiveness was found to be matrix metalloproteinase (MMP)-13. Further, the inhibitory effects of shikonin on cell invasiveness were completely abolished in MMP13-overexpressing OS cells. Together, these data suggest that shikonin may suppress OS invasiveness through MMP13 suppression. Thus, our data highlight a previous unappreciated role for shikonin in suppressing OS cell metastasis.
authors.affiliation: Department of Orthopedics, Guizhou Province Osteological Hospital, 25 Shachong S Rd, Guiyang, 550007, China.
authors.forename: Biyong
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of Orthopedics, Guizhou Province Osteological Hospital, 25 Shachong S Rd, Guiyang, 550007, China. Bing_qiu15@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Qiu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis* / Q000235:genetics; D009285:Naphthoquinones / Q000008:administration & dosage*; D009361:Neoplasm Invasiveness / Q000235:genetics; D012516:Osteosarcoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology
chemical_list: D009285:Naphthoquinones; C016101:shikonin; D053509:Matrix Metalloproteinase 13
keywords: Matrix metalloproteinase (MMP)-13; Osteosarcoma (OS); Shikonin