title: Engineering Yarrowia lipolytica for production of medium-chain fatty acids.
abstract: Lipids are naturally derived products that offer an attractive, renewable alternative to petroleum-based hydrocarbons. While naturally produced long-chain fatty acids can replace some petroleum analogs, medium-chain fatty acid would more closely match the desired physical and chemical properties of currently employed petroleum products. In this study, we engineered Yarrowia lipolytica, an oleaginous yeast that naturally produces lipids at high titers, to produce medium-chain fatty acids. Five different acyl-acyl carrier protein (ACP) thioesterases with specificity for medium-chain acyl-ACP molecules were expressed in Y. lipolytica, resulting in formation of either decanoic or octanoic acid. These novel fatty acid products were found to comprise up to 40 % of the total cell lipids. Furthermore, the reduction in chain length resulted in a twofold increase in specific lipid productivity in these engineered strains. The medium-chain fatty acids were found to be incorporated into all lipid classes.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA.
authors.forename: Charles D
authors.initials: CD
authors.lastname: Rutter
authors.forename: Shuyan
authors.initials: S
authors.lastname: Zhang
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Rao
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics*; D002210:Caprylates / Q000378:metabolism; D003652:Decanoic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D015870:Gene Expression; D060847:Metabolic Engineering; D010170:Palmitoyl-CoA Hydrolase / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D025062:Yarrowia / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000213:Acyl Carrier Protein; D002210:Caprylates; D003652:Decanoic Acids; D005227:Fatty Acids; D011994:Recombinant Proteins; C031071:decanoic acid; D010170:Palmitoyl-CoA Hydrolase; C031492:octanoic acid