title: Role of the stromal vascular fraction from adipose tissue in association with a phosphocalcic scaffold in bone regeneration in an irradiated area.
abstract: BACKGROUND
  Osteoradionecrosis of the jaw is a major side-effect of radiotherapy used in the treatment of squamous cell carcinomas of the upper aerodigestive tract. The standard reconstruction procedure is a free flap transfer of autogenous bone. A new approach using a tissue engineering strategy has shown that total bone marrow (TBM) associated with biphasic calcium phosphate (BCP) is the best combination for bone regeneration in an irradiated area. Recently, the stromal vascular fraction from adipose tissue (SVF) was described as an alternative to TBM for promoting new bone formation. The aim of this study was to identify the capacity of a freshly isolated SVF to induce new bone formation in an irradiated area.
  
  
  METHODS
  Four weeks after irradiation of the hind limbs of 15 rats, bone defects were created and filled with either SVF or TBM with and without BCP.
  
  
  RESULTS
  Three weeks after the implantations, analysis showed that the BCP-TBM mixture improved new bone formation after radiation (p < 0.05). The BCP-SVF association induced significant neoangiogenesis but failed to enhance new bone formation.
  
  
  CONCLUSIONS
  The BCP-SVF mixture was insufficient to enhance new bone formation in the irradiated area, suggesting that the role of the environment might be crucial for ossification.
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Service d'oto-rhino-laryngologie et de chirurgie cervico-faciale, 1 place Alexis Ricordeau, 44093 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Thery
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France; Université de Nantes, UFR Odontologie, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Bléry
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Service d'oto-rhino-laryngologie et de chirurgie cervico-faciale, 1 place Alexis Ricordeau, 44093 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Malard
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Sourice
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France; Centre Hospitalier Universitaire de Nantes, Service de stomatologie et de chirurgie maxillo-faciale, 1 place Alexis Ricordeau, 44093 Nantes Cedex 1, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Corre
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France; Université de Nantes, UFR Odontologie, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.affiliation: INSERM, UMRS 791 (Head: Pr Pierre Weiss, DDS-PhD), Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Service d'oto-rhino-laryngologie et de chirurgie cervico-faciale, 1 place Alexis Ricordeau, 44093 Nantes Cedex 1, France; Centre Hospitalier Universitaire de Nantes, Pôle hospitalo-universitaire 4 OTONN, France. Electronic address: florent.espitalier@chu-nantes.fr.
authors.forename: Florent
authors.initials: F
authors.lastname: Espitalier
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000818:Animals; D001861:Bone Regeneration; D007568:Jaw / Q000473:pathology*; D010025:Osteoradionecrosis / Q000473:pathology / Q000628:therapy*; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
keywords: Adipose tissue; Bone marrow graft; Calcium phosphate scaffold; Irradiated bone regeneration; Stromal vascular fraction