title: Role of exon 7 PTEN Gene in Endometrial Carcinoma.
abstract: BACKGROUND
  Endometrial carcinoma is the most common malignant tumor of the female genital tract and the fourth most common cancer in Iranian women after breast, colorectal and lung cancers. Various genetic alterations appear to be early events in the pathogenesis of endometrial carcinoma and it seems that PTEN is the most commonly mutated gene in the endometrioid subtype. The aim of the present study was to investigate the correlation between mutations in exon 7 of PTEN gene and endometrial carcinoma.
  
  
  METHODS
  Seventy-five patients with endometrial carcinoma and 75 females whose underwent hysterectomy for non tumoral indication were selected for evaluation of PTEN mutations in exon 7 by PCR-SSCP and sequencing. Correlations between the frequency and type of mutation and the pathologic findings of the cancer (tumor subtype, stage and grade) were assessed.
  
  
  RESULTS
  All of the samples were obtained from Iranian patients. 60 % (45 cases) of the tumors were endometriod and 40% (30 cases) were of serous type. The grade distributions of the 75 cases according to the FIGO staging system were as follows: low grade, 20 cases; high grade 55 cases, low stage, 41 cases; high stage 34 cases. For exon 7 of the PTEN gene, the analysis showed that there were no mutations in our cases.
  
  
  CONCLUSIONS
  Our findings in the present study suggest that exon 7 of PTEN does not play any significant role in the development of endometrial carcinoma in Iranian cases.
authors.affiliation: Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran E-mail : dastranjt@gmail.com,akbarzadehab@tbzmed.ac.ir.
authors.forename: Leila
authors.initials: L
authors.lastname: Kafshdooz
authors.forename: Taiebeh
authors.initials: T
authors.lastname: Kafshdooz
authors.forename: Ali Dastranj
authors.initials: AD
authors.lastname: Tabrizi
authors.forename: Seyyed Mojtaba
authors.initials: SM
authors.lastname: Mohaddes Ardabili
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.forename: Jalal
authors.initials: J
authors.lastname: Gharesouran
authors.forename: Morteza
authors.initials: M
authors.lastname: Ghojazadeh
authors.forename: Safar
authors.initials: S
authors.lastname: Farajnia
mesh_terms: D016022:Case-Control Studies; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D004717:Endometrium / Q000378:metabolism*; D005091:Exons / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007044:Hysterectomy; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis
chemical_list: D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human