title: [Effect of Pulsed Electromagnetic Fields on Osteogenic Differentiation and Wnt/β-catenin Signaling Pathway in Rat Bone Marrow Mesenchymal Stem Cells].
abstract: OBJECTIVE
  To investigate the effect of pulsed electromagnetic tields (FEMFs) on osteogemc differentiation and Wnt/β-catenin signaling pathway in rat bone marrow mesenchymal stem cells (BMSCs).
  
  
  METHODS
  Rat BMSCs were isolated and the passage 3 cells were divided into 3 groups. Cells were cultured in LG-DMEM complete medium for 1 d to ensure fully adherent. Then, change the medium. Cells were maintained in complete medium (Control group) or in osteo-induction medium (OM group). The cells in PEMFs group were cultured in complete medium and exposed to 8 Hz, 3. 8 mT PEMF stimulation for 40 min/d. The intervention lasted for 21 d. Cell proliferation activity was determined by using MTT. The effects of PEMF onosteogenic differentiation were assessed by ALP and Alizarin Red S staining. Various osteoblast-relevant genes and genes of Wnt/β-catenin signaling were analyzed by quantitative real-time RT-PCR.
  
  
  RESULTS
  We. found that OM could significantly promote the proliferation of BMSC at 7 d, 14 d, 21 d (P<0. 05), but the effect was not obviously found in PEMFs group. For osteogenic differentiation, the positive rates of ALP or Alizarin Red S staining were detected higher in PEMFs/ OM group (P < 0. 05). Quantitative RT-PCR revealed PEMFs or OM could increase mRNA levels of Wnt1, Wnt3a, LRP5, β-catenin, BMP-2, Runx2, ALP, OC at special time point (P<0. 05). Compared to OM group, PEMFs have a lower expression in each detection, but the trends were consistent.
  
  
  CONCLUSIONS
  PEMFs (8 Hz, 3. 8 mT) could induce the osteogenic differentiation of rat BMSCs via activating Wnt/β-catenin signaling pathway.
authors.forename: Yu-jing
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Chuan
authors.initials: C
authors.lastname: Liu
authors.forename: Qiao-dan
authors.initials: QD
authors.lastname: Ji
authors.forename: Xiao-tian
authors.initials: XT
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.forename: Cheng-qi
authors.initials: CQ
authors.lastname: He
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D051381:Rats; D060449:Wnt Signaling Pathway; D051155:Wnt1 Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495268:Ctnnb1 protein, rat; D051155:Wnt1 Protein; C495245:Wnt1 protein, rat; D051176:beta Catenin