title: Independent Neuronal Origin of Seizures and Behavioral Comorbidities in an Animal Model of a Severe Childhood Genetic Epileptic Encephalopathy.
abstract: The childhood epileptic encephalopathies (EE's) are seizure disorders that broadly impact development including cognitive, sensory and motor progress with severe consequences and comorbidities. Recently, mutations in DNM1 (dynamin 1) have been implicated in two EE syndromes, Lennox-Gastaut Syndrome and Infantile Spasms. Dnm1 encodes dynamin 1, a large multimeric GTPase necessary for activity-dependent membrane recycling in neurons, including synaptic vesicle endocytosis. Dnm1Ftfl or "fitful" mice carry a spontaneous mutation in the mouse ortholog of DNM1 and recapitulate many of the disease features associated with human DNM1 patients, providing a relevant disease model of human EE's. In order to examine the cellular etiology of seizures and behavioral and neurological comorbidities, we engineered a conditional Dnm1Ftfl mouse model of DNM1 EE. Observations of Dnm1Ftfl/flox mice in combination with various neuronal subpopulation specific cre strains demonstrate unique seizure phenotypes and clear separation of major neurobehavioral comorbidities from severe seizures associated with the germline model. This demonstration of pleiotropy suggests that treating seizures per se may not prevent severe comorbidity observed in EE associated with dynamin-1 mutations, and is likely to have implications for other genetic forms of EE.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Samuel K
authors.initials: SK
authors.lastname: Asinof
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Stacey J
authors.initials: SJ
authors.lastname: Sukoff Rizzo
authors.affiliation: Biomedical Sciences Graduate Program, University of California, San Diego, San Diego, California, United States of America.
authors.forename: Alexandra R
authors.initials: AR
authors.lastname: Buckley
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Beyer
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Verity A
authors.initials: VA
authors.lastname: Letts
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Wayne N
authors.initials: WN
authors.lastname: Frankel
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Boumil
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D004195:Disease Models, Animal; D034284:Dynamin I / Q000235:genetics* / Q000378:metabolism; D004569:Electroencephalography; D004827:Epilepsy / Q000453:epidemiology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007223:Infant; D065768:Lennox Gastaut Syndrome / Q000453:epidemiology / Q000235:genetics; D008297:Male; D008817:Mice, Mutant Strains; D009474:Neurons / Q000473:pathology; D010641:Phenotype; D016548:Prosencephalon / Q000378:metabolism / Q000503:physiopathology; D013036:Spasms, Infantile / Q000453:epidemiology / Q000235:genetics; D009435:Synaptic Transmission
chemical_list: D034284:Dynamin I