title: [Inhibitory effects of Hes1 on acute myeloid leukemia cells].
abstract: OBJECTIVE
  To elucidate the impact of Hes1 on the proliferation and apoptosis of acute myeloid leukemia (AML) cells.
  
  
  METHODS
  The expression levels of Hes1 and p21 in AML patient samples and myeloid leukemia cell lines were analyzed by real-time PCR. Hes1 was up-regulated by retrovirus transfection in AML cell lines and the proliferation capacity were assayed by MTT, cell cycle by Hoechst/PY, apoptosis by AnnexinV.
  
  
  RESULTS
  The expression of Hes1 in primary AML cells and HL-60, U937, KG1a cell lines were 0.67 ± 0.24, 0.59 ± 0.43, 0.42 ± 0.03, and 0.32 ± 0.26, respectively, and p21 were 0.54 ± 0.01, 0.44 ± 0.12, 0.36 ± 0.12, and 0.59 ± 0.43, respectively. Hes1 expression levels after transduction in HL-60, U937, KG1a were 4.9 ± 0.2, 5.2 ± 0.4, 5.8 ± 0.5, respectively. Induced activation of Hes1 led to AML cells growth arrest and apoptosis, which was associated with an enhanced p21 expression. Besides, activated Hes1 led to AML cells growth inhibition in vivo.
  
  
  CONCLUSIONS
  Hes1 could mediate growth arrest and apoptosis in AML cells, which may be a novel target for AML.
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Institute and Hospital. Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin　300060, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tian
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Institute and Hospital. Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin　300060, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Institute and Hospital. Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin　300060, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Institute and Hospital. Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin　300060, China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Institute and Hospital. Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin　300060, China.
authors.forename: Fulian
authors.initials: F
authors.lastname: Qu
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Institute and Hospital. Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin　300060, China.
authors.forename: Yizhuo
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins; D002453:Cell Cycle; D045744:Cell Line, Tumor; D018398:Homeodomain Proteins; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D000072056:Transcription Factor HES-1; D015854:Up-Regulation
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D000072056:Transcription Factor HES-1; C078493:HES1 protein, human