title: Downregulation of miR-410 targeting the cyclin B1 gene plays a role in pituitary gonadotroph tumors.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs that act as posttranscriptional regulators of gene expression, and are frequently altered in human neoplasias. Here, we have analyzed the miRNA expression profile of human gonadotroph adenomas versus normal pituitary tissue using a miRNACHIP microarray. We demonstrate that miRNA-410 is downregulated in gonadotroph adenomas when compared with normal pituitary gland. We validate CCNB1 as target of miRNA-410 since its overexpression reduces CCNB1 at protein and mRNA levels, decreasing cell proliferation. In conclusion, our study suggess that the downregulation of miRNA-410 plays a role in the behavior of gonadotroph tumors.
authors.affiliation: a Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche ; Scuola di Medicina e Chirurgia di Napoli; Università degli Studi di Napoli "Federico II" ; Naples , Italy.
authors.forename: Paula
authors.initials: P
authors.lastname: Müssnich
authors.forename: Gerald
authors.initials: G
authors.lastname: Raverot
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Jaffrain-Rea
authors.forename: Filippo
authors.initials: F
authors.lastname: Fraggetta
authors.forename: Anne
authors.initials: A
authors.lastname: Wierinckx
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Trouillas
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
authors.forename: Daniela
authors.initials: D
authors.lastname: D'Angelo
mesh_terms: D020413:3' Untranslated Regions; D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D001665:Binding Sites; D049109:Cell Proliferation; D056744:Cyclin B1 / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; C534699:CCNB1 protein, human; D056744:Cyclin B1; C568527:MIRN410 microRNA, human; D035683:MicroRNAs
keywords: cyclin B1; miRNA-410; pituitary adenomas