title: Indole: a signaling molecule or a mere metabolic byproduct that alters bacterial physiology at a high concentration?
abstract: Indole is an organic compound that is widespread in microbial communities inhabiting diverse habitats, like the soil environment and human intestines. Measurement of indole production is a traditional method for the identification of microbial species. Escherichia coli can produce millimolar concentrations of indole in the stationary growth phase under nutrient-rich conditions. Indole has received considerable attention because of its remarkable effects on various biological functions of the microbial communities, for example, biofilm formation, motility, virulence, plasmid stability, and antibiotic resistance. Indole may function as an intercellular signaling molecule, like a quorum-sensing signal. Nevertheless, a receptor system for indole and the function of this compound in coordinated behavior of a microbial population (which are requirements for a true signaling molecule) have not yet been confirmed. Recent findings suggest that a long-known quorum-sensing regulator, E. coli's SdiA, cannot recognize indole and that this compound may simply cause membrane disruption and energy reduction, which can lead to various changes in bacterial physiology including unstable folding of a quorum-sensing regulator. Indole appears to be responsible for acquisition of antibiotic resistance via the formation of persister cells and activation of an exporter. This review highlights and summarizes the current knowledge about indole as a multitrophic molecule among bacteria, together with recently identified new avenues of research.
authors.affiliation: Laboratory of Molecular Environmental Microbiology, Department of Environmental Sciences and Ecological Engineering, Korea University, Seoul, 136-713, Republic of Korea.
authors.forename: Jisun
authors.initials: J
authors.lastname: Kim
authors.forename: Woojun
authors.initials: W
authors.lastname: Park
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D018441:Biofilms / Q000254:growth & development; D004926:Escherichia coli / Q000502:physiology; D029968:Escherichia coli Proteins / Q000378:metabolism / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007211:Indoles / Q000378:metabolism*; D010957:Plasmids; D053038:Quorum Sensing; D015398:Signal Transduction; D015534:Trans-Activators / Q000502:physiology; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D007211:Indoles; D015534:Trans-Activators; C490806:sdiA protein, E coli; C030374:indole