title: [Follicular Helper T Cells and Expression of PD-1 in Mice with Myelodysplastic Syndrome].
abstract: OBJECTIVE
  To investigate the complete blood count, morphological changes, follicular T helper (Tfh) cells and expression of PD-1 in bone marrow and spleen of mice with myelodysplastic syndrome(MDS) and to explore their significance in pathogenesis of MDS.
  
  
  METHODS
  The 10 male NUP98-HOXD13 transgenic mice and 10 male homologous wild-type C57BL/6J mice were used for experments. The complete blood count, morphological change of NUP98-HOXD13 transgenic mice and wild-type C57BL/6J were detected by routine methods. The level of Tfh cells and expression of PD-1 in bone marrow and spleen were measured by flow cytometry. The PD-1 mRNA of bone marrow mononuclear cells and spleen cells were analyzed by real-time PCR method.
  
  
  RESULTS
  The counts of RBC, neutrophile and platelet in above- mentioned transgenic mice were less than that in wild type C57BL/6J mice. As compared with wild type C57BL/6J mice, the morphology of RBC and platelet in transgenic mice was some abnormal, including bi-nucleated erythrocytes, ringed mucleated neutrophil and erythroblastic islands. The count of Tfh cells in transgenic mice was less than that in wild type mice, but the expression of PD-1 was higher. The expression of BMMNC PD-1 mRNA was obviously higher than that in wild type mice.
  
  
  CONCLUSIONS
  The pancytopenia and dysplasia, decrease of Tfh cells and increase of PD-1 expression have been observed in NUP98-HOXD13 transgenic mice, which may be one of important reasons for promoting malignant clone and leading to impair anti immune respones.
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Ning-Bo
authors.initials: NB
authors.lastname: Cui
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Hua-Quan
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjing 300192, China.
authors.forename: De-Guan
authors.initials: DG
authors.lastname: Li
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjing 300192, China.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Yan
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjing 300192, China.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Meng
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China. E-mail: shaozonghong@sina.com.
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Shao
mesh_terms: D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009190:Myelodysplastic Syndromes; D010198:Pancytopenia; D061026:Programmed Cell Death 1 Receptor; D060888:Real-Time Polymerase Chain Reaction; D006377:T-Lymphocytes, Helper-Inducer
chemical_list: C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor