title: EHEC Genomics: Past, Present, and Future.
abstract: This article examines the role of genomics in the understanding and identification of O157:H7 enterohemorrhagic Escherichia coli (EHEC). We highlight the development of novel molecular typing systems that are based on the genomic sequence that has been generated for this pathotype. The genomic comparisons of EHEC to other E. coli strains highlight the close relatedness of the O157 and O55 isolates and also identify other non-O157 clades of isolates that appear to have a different genomic history. Analysis within the EHEC isolates must be completed on a fine scale using whole-genome sequence-based approaches to assess both the conserved and lateral acquired gene content. The plethora of genomic data for EHEC isolates has provided the ability to examine this pathotype in detail, which has provided opportunities for novel surveillance, detection, and diagnostics.
authors.affiliation: Department of Biology, and South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, TX 78249.
authors.forename: Shah M
authors.initials: SM
authors.lastname: Sadiq
authors.affiliation: Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.
authors.forename: Tracy H
authors.initials: TH
authors.lastname: Hazen
authors.affiliation: Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.
authors.forename: David A
authors.initials: DA
authors.lastname: Rasko
authors.affiliation: Department of Biology, and South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, TX 78249.
authors.forename: Mark
authors.initials: M
authors.lastname: Eppinger
mesh_terms: D054324:Enterohemorrhagic Escherichia coli / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D005827:Genetics, Microbial / Q000639:trends; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D008829:Microbiology / Q000639:trends; D058889:Molecular Typing