title: A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine.
abstract: Herein we report the use of bromomaleimides for the construction of stable albumin conjugates via conjugation to its native, single accessible, cysteine followed by hydrolysis. Advantages over the classical maleimide approach are highlighted in terms of quantitative hydrolysis and absence of undesirable retro-Michael deconjugation.
authors.affiliation: Department of Chemistry, University College London, London, WC1H 0AJ, UK. j.r.baker@ucl.ac.uk v.chudasama@ucl.ac.uk.
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.forename: Mikael B
authors.initials: MB
authors.lastname: Caspersen
authors.forename: Eifion
authors.initials: E
authors.lastname: Robinson
authors.forename: Maurício
authors.initials: M
authors.lastname: Morais
authors.forename: Antoine
authors.initials: A
authors.lastname: Maruani
authors.forename: João P M
authors.initials: JP
authors.lastname: Nunes
authors.forename: Karl
authors.initials: K
authors.lastname: Nicholls
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Saxton
authors.forename: Stephen
authors.initials: S
authors.lastname: Caddick
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
authors.forename: Vijay
authors.initials: V
authors.lastname: Chudasama
mesh_terms: D000418:Albumins / Q000737:chemistry*; D057930:Click Chemistry; D003545:Cysteine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008298:Maleates / Q000737:chemistry; D013058:Mass Spectrometry; D017433:Protein Structure, Secondary; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000418:Albumins; D008298:Maleates; D013438:Sulfhydryl Compounds; D003545:Cysteine; C008311:maleamic acid