title: The complete mitochondrial genome of the killifish Orestias sp. (Cyprinodontiformes, Cyprinodontidae) from the high Andean range.
abstract: The killifish genus Orestias is endemic to freshwater ecosystems in the High Andes of Peru, Bolivia and Chile. Phylogenetic and phylogeographic studies with partial mitochondrial genes have failed to resolve relationship among species, needing more comprehensive approaches. In this study, we described the complete mitochondrial genome of Orestias sp. from Lirima, northern Chile, with the aim to provide useful data for phylogenetic purposes and species delimitation. The mitochondrial genome was assembled with 2.6 million of reads obtained through an Ion Torrent (chip 318) sequencer. The circular sequence of 16,617 bp showed the following nucleotide composition: A, 26.7%, C, 27.1%, G, 17.0%, and T, 29.2%. Gene composition and structure were similar to other fish sequences available, and comprised 13 protein-coding genes, 12S and 16S rRNA, 22 tRNA genes, and a control region.
authors.affiliation: a Fish and Isotope Ecology Laboratory, Instituto de Ciencias Naturales Alexander von Humboldt, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta , Antofagasta , Chile .
authors.forename: Claudio
authors.initials: C
authors.lastname: Quezada-Romegialli
authors.affiliation: b Departamento de Ciencias Ecológicas , Universidad de Chile , Santiago , Chile .|c Escuela de Medicina Veterinaria sede Talca, Facultad de Recursos Naturales y Medicina Veterinaria, Universidad Santo Tomás , Talca , Chile , and.
authors.forename: Claudia Jimena
authors.initials: CJ
authors.lastname: Guerrero
authors.affiliation: b Departamento de Ciencias Ecológicas , Universidad de Chile , Santiago , Chile .|d Instituto de Ecología y Biodiversidad, Núcleo Milenio de Ecología y Manejo Sustentable de Islas Oceánicas, Universidad de Chile , Santiago , Chile.
authors.forename: David
authors.initials: D
authors.lastname: Véliz
authors.affiliation: b Departamento de Ciencias Ecológicas , Universidad de Chile , Santiago , Chile .
authors.forename: Irma
authors.initials: I
authors.lastname: Vila
mesh_terms: D000818:Animals; D005618:Fresh Water; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D007695:Killifishes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny
keywords: Altiplano; NGS; freshwater fish; next-generation sequencing