title: Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts.
abstract: The aim of the work was to test a relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7 cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 55 were identified by matrix-assisted laser desorption/ionization - time of flight tandem mass spectrometry. Clinically relevant proteins associated with celiac disease, wheat dependent exercise induced anaphylaxis, baker's asthma, and food allergy, were detected in 24 2-DE spots. However, alcohol-soluble gliadins were not detected with this approach. The comparison with a recent quantitative study suggested that gel-based and gel-free proteomics approaches are complementary for the detection and quantification of clinically relevant proteins in wheat grain.
authors.affiliation: Department of Developmental and Reproduction Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences Nitra, Slovakia ; Faculty of Natural Sciences, Comenius University Bratislava, Slovakia.
authors.forename: Sona
authors.initials: S
authors.lastname: Fekecsová
authors.affiliation: Institute of Virology, Slovak Academy of Sciences Bratislava, Slovakia.
authors.forename: Maksym
authors.initials: M
authors.lastname: Danchenko
authors.affiliation: Department of Developmental and Reproduction Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences Nitra, Slovakia.
authors.forename: Lubica
authors.initials: L
authors.lastname: Uvackova
authors.affiliation: Institute of Virology, Slovak Academy of Sciences Bratislava, Slovakia.
authors.forename: Ludovit
authors.initials: L
authors.lastname: Skultety
authors.affiliation: Department of Developmental and Reproduction Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences Nitra, Slovakia ; Institute of Virology, Slovak Academy of Sciences Bratislava, Slovakia.
authors.forename: Martin
authors.initials: M
authors.lastname: Hajduch
keywords: 2-DE; 7 cm IPG; MALDI-TOF/TOF; Triticum aestivum; allergen; gel-based; grain; quantification