title: Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus.
abstract: The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenBank accession numbers: KC117456 to KC117470). The number of alleles per locus ranged from 5 to 12 among 40 individuals, and the average observed and expected heterozygosities were 0.344 and 0.828, respectively. Three loci showed significant deviations from Hardy-Weinberg equilibrium. These polymorphic loci could provide a valuable tool for investigations of the population genetics, phylogeography, and conservation genetics of P. dabryanus.
authors.affiliation: Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.affiliation: Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.affiliation: Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.affiliation: Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: X
authors.initials: X
authors.lastname: Bai
authors.affiliation: Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, China caoxiaojuan@mail.hzau.edu.cn.
authors.forename: X
authors.initials: X
authors.lastname: Cao
mesh_terms: D000483:Alleles; D000818:Animals; D002681:China; D003531:Cypriniformes / Q000235:genetics*; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic