title: Confocal microscopy characterization of BRAFV600E mutated melanomas.
abstract: Thanks to modern techniques, molecular signatures for melanoma are now identifiable and have opened new horizons in the treatment of metastatic disease with molecular-targeted therapies. We distinguish different melanoma subtypes on the basis of genetic mutations such as BRAFV600E and we can therefore hypothesize the existence of corresponding morphological patterns that might be detected in vivo by noninvasive diagnostic tools such as dermoscopy and confocal microscopy. Eight BRAFV600E mutated melanomas (six primary and two metastases) were collected, matched in terms of age, sex, and thickness wild-type controls, and analyzed. In this preliminary study, regression, corresponding to fibrosis and melanophages in the dermis, was the predominant pattern and was also observed confocally when dermoscopy showed no peppering. In particular, confocal microscopy could not only detect regression but also provided a semiquantitative analysis of its grade through the count of melanophages. Confocal microscopy can be proposed as a useful tool in the preliminary screening and characterization of BRAFV600E mutated melanomas, providing new insights for patients' screening and follow-up.
authors.affiliation: Departments of aDermatology bClinical and Diagnostic Medicine and Public Health, University of Modena and Reggio Emilia, Modena, Italy cDepartment of Dermatology, Ludwig Maximilian University of Munich, Munich, Germany.
authors.forename: Cristel
authors.initials: C
authors.lastname: Ruini
authors.forename: Marco
authors.initials: M
authors.lastname: Manfredini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pellacani
authors.forename: Victor D
authors.initials: VD
authors.lastname: Mandel
authors.forename: Aldo
authors.initials: A
authors.lastname: Tomasi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ponti
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D046169:Dermoscopy / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D018613:Microscopy, Confocal; D008875:Middle Aged; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf