title: Cyclin B3 controls anaphase onset independent of spindle assembly checkpoint in meiotic oocytes.
abstract: Cyclin B3 is a relatively new member of the cyclin family whose functions are little known. We found that depletion of cyclin B3 inhibited metaphase-anaphase transition as indicated by a well-sustained MI spindle and cyclin B1 expression in meiotic oocytes after extended culture. This effect was independent of spindle assembly checkpoint activity, since both Bub3 and BubR1 signals were not observed at kinetochores in MI-arrested cells. The metaphase I arrest was not rescued by either Mad2 knockdown or cdc20 overexpression, but it was rescued by securin RNAi. We conclude that cyclin B3 controls the metaphase-anaphase transition by activating APC/C(cdc20) in meiotic oocytes, a process that does not rely on SAC activity.
authors.affiliation: a Institute of Reproductive Sciences; College of Animal Science and Technology; Qingdao Agricultural University ; Qingdao , China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Qi
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Ma
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000705:Anaphase; D000818:Animals; D002478:Cells, Cultured; D019926:Cyclin B / Q000502:physiology*; D005260:Female; D059566:M Phase Cell Cycle Checkpoints; D008540:Meiosis; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000502:physiology*
chemical_list: C503695:Ccnb3 protein, mouse; D019926:Cyclin B
keywords: Cyclin B3; SAC; anaphase initiation; meiosis; oocyte