title: New Metrics for Evaluating Viral Respiratory Pathogenesis.
abstract: Viral pathogenesis studies in mice have relied on markers of severe systemic disease, rather than clinically relevant measures, to evaluate respiratory virus infection; thus confounding connections to human disease. Here, whole-body plethysmography was used to directly measure changes in pulmonary function during two respiratory viral infections. This methodology closely tracked with traditional pathogenesis metrics, distinguished both virus- and dose-specific responses, and identified long-term respiratory changes following both SARS-CoV and Influenza A Virus infection. Together, the work highlights the utility of examining respiratory function following infection in order to fully understand viral pathogenesis.
authors.affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
authors.forename: Vineet D
authors.initials: VD
authors.lastname: Menachery
authors.affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Gralinski
authors.affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Baric
authors.affiliation: Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
authors.forename: Martin T
authors.initials: MT
authors.lastname: Ferris
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D004285:Dogs; D053118:Influenza A Virus, H1N1 Subtype; D008168:Lung / Q000473:pathology / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000503:physiopathology*; D010993:Plethysmography, Whole Body; D011237:Predictive Value of Tests; D045473:Severe acute respiratory syndrome-related coronavirus; D045169:Severe Acute Respiratory Syndrome / Q000503:physiopathology*; D014709:Vero Cells; D019562:Viral Load; D014779:Virus Replication / Q000502:physiology