title: Effect of polyamine metabolism inhibitors on Lewis lung carcinoma growth and metastasis.
abstract: OBJECTIVE
  To study the influence of polyamine metabolism inhibitors on the growth, metastasis and ornithine decarboxylase (ODC) activity of Lewis lung carcinoma.
  
  
  METHODS
  Experiments were performed on female mice C57Bl/6 with Lewis lung carcinoma. Nω-hydroxy-nor-arginine (nor-NOHA) and α-difluoromethylornithine (DFMO) were used as arginase and ODC inhibitors, correspondently. Inhibition of tumor growth was calculated by comparison of tumor volume in the treated and control groups. The average number of metastases per animal in the group and the average volume of pulmonary metastases per animal in the group have been determined. Determination of ODC - the key enzyme of the polyamine synthesis - in the samples of experimental tumors was performed by method of Luqman S.
  
  
  RESULTS
  Administration of DFMO or it's combination with nor-NOHA resulted in the decrease of tumor growth rate, number and volume of lung metastases and was accompanied with reduced ODC activity in tumor tissue.
  
  
  CONCLUSIONS
  Modifiers of polyamine metabolism may be considered as promising targeted cancer therapy.
authors.affiliation: RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: О А
authors.initials: ОА
authors.lastname: Samoilenko
authors.affiliation: RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: O A
authors.initials: OA
authors.lastname: Milinevska
authors.affiliation: RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: O V
authors.initials: OV
authors.lastname: Karnaushenko
authors.affiliation: RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: V A
authors.initials: VA
authors.lastname: Shlyakhovenko
authors.affiliation: RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
authors.forename: S P
authors.initials: SP
authors.lastname: Zaletok
mesh_terms: D000818:Animals; D001119:Arginase / Q000037:antagonists & inhibitors; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000556:secondary; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D000518:Eflornithine / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D047368:Tumor Burden / Q000187:drug effects
chemical_list: C404735:N(omega)-hydroxynorarginine; D001120:Arginine; D001119:Arginase; D000518:Eflornithine