title: Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction.
abstract: Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The 20, 54, and 87th generations of CEK-adapted IBV-Sczy3 strains were used to infect CEK cells, and then nucleic copy numbers in the samples of supernatant collected at 12, 24, 36, 48, 60, and 72 h were detected. The results showed that the rapid growth period of the virus load of all the 3 generations was approximately 12-36 h post-infection; the peak of the virus load appeared at 36 h post-infection and then decreased gradually in the order of 20th > 54th > 87th for the 3 generations of CEK-adapted strains; the dynamic change curve of the IBV load in the supernatant of primary CEK cells showed a single peak. The results of this study provide a useful reference for CEK-adapted IBV field strains and the production of CEK-attenuated IBV vaccine.
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Wei
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: M P
authors.initials: MP
authors.lastname: Guo
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Duan
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: N L
authors.initials: NL
authors.lastname: Zou
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Q G
authors.initials: QG
authors.lastname: Yan
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: X T
authors.initials: XT
authors.lastname: Wen
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Cao
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China hyong601@163.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000276:immunology / Q000821:virology; D003333:Coronaviridae Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D001351:Infectious bronchitis virus / Q000276:immunology* / Q000472:pathogenicity; D007668:Kidney / Q000276:immunology / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D061251:Primary Cell Culture; D014613:Vaccines, Attenuated / Q000276:immunology* / Q000627:therapeutic use; D019562:Viral Load / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines