title: Sensor Based on Aptamer Folding to Detect Low-Molecular Weight Analytes.
abstract: Aptamers have emerged as promising biorecognition elements in the development of biosensors. The present work focuses on the application of quartz crystal microbalance with dissipation monitoring (QCM-D) for the enantioselective detection of a low molecular weight target molecule (less than 200 Da) by aptamer-based sensors. While QCM-D is a powerful technique for label-free, real-time characterization and quantification of molecular interactions at interfaces, the detection of small molecules interacting with immobilized receptors still remains a challenge. In the present study, we take advantage of the aptamer conformational changes upon the target binding that induces displacement of water acoustically coupled to the sensing layer. As a consequence, this phenomenon leads to a significant enhancement of the detection signal. The methodology is exemplified with the enantioselective recognition of a low molecular weight model compound, L-tyrosinamide (L-Tym). QCM-D monitoring of L-Tym interaction with the aptamer monolayer leads to an appreciable signal that can be further exploited for analytical purposes or thermodynamics studies. Furthermore, in situ combination of QCM-D with spectroscopic ellipsometry unambiguously demonstrates that the conformational change induces a nanometric decrease of the aptamer monolayer thickness. Since QCM-D is sensitive to the whole mass of the sensing layer including water that is acoustically coupled, a decrease in thickness of the highly hydrated aptamer layer induces a sizable release of water that can be easily detected by QCM-D.
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Alina
authors.initials: A
authors.lastname: Osypova
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Dhruv
authors.initials: D
authors.lastname: Thakar
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Dejeu
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Hugues
authors.initials: H
authors.lastname: Bonnet
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Angéline
authors.initials: A
authors.lastname: Van der Heyden
authors.affiliation: §CIC biomaGUNE, 20009 Donostia-San Sebastian, Spain.
authors.forename: Galina V
authors.initials: GV
authors.lastname: Dubacheva
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.|§CIC biomaGUNE, 20009 Donostia-San Sebastian, Spain.|∥Max-Planck-Institute for Intelligent Systems, 70569 Stuttgart, Germany.
authors.forename: Ralf P
authors.initials: RP
authors.lastname: Richter
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Defrancq
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Spinelli
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Liliane
authors.initials: L
authors.lastname: Coche-Guérente
authors.affiliation: †Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|‡CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Labbé
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D008970:Molecular Weight; D058525:Quartz Crystal Microbalance Techniques; D054852:Small Molecule Libraries / Q000032:analysis; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D054852:Small Molecule Libraries; C036387:tyrosinamide; D014443:Tyrosine