title: p38α controls self-renewal and fate decision of neurosphere-forming cells in adult hippocampus.
abstract: Neural stem cells (NSC) from the adult hippocampus easily lose their activity in vitro. Efficient in vitro expansion of adult hippocampus-derived NSC is important for generation of tools for research and cell therapy. Here, we show that a single copy disruption or pharmacological inhibition of p38α enables successful long-term neurosphere culture of adult mouse hippocampal cells. Expanded neurospheres with high proliferative activity differentiated into the three neuronal lineages under differentiating conditions. Thus, inhibition of p38α can maintain adult hippocampal NSC activity in vitro.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Yoshioka
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Namiki
authors.affiliation: RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Sudo
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Kasuya
keywords: Adult hippocampus; DG, dentate gyrus; NSC, neural stem cells; Neural stem cell; Neurosphere; RGL, radial glia-like cells; SGZ, subgranular zone; SVZ, subventricular zone; p38α