title: Cyto/hemocompatible magnetic hybrid nanoparticles (Ag2S-Fe3O4) with luminescence in the near-infrared region as promising theranostic materials.
abstract: Small hybrid nanoparticles composed of highly biocompatible Ag2S quantum dots (QD) emitting in the near-infrared region and superparamagnetic iron oxide (SPION) are produced in a simple extraction method utilizing ligand exchange mechanism. Hybrid nanoparticles luminesce at the same wavelength as the parent QD, therefore an array of hybrid nanoparticles with emission between 840 and 912nm were easily produced. Such hybrid structures have (1) strong luminescence in the medical imaging window eliminating the autofluoresence of cells as effective optical probes, (2) strong magnetic response for magnetic targeting and (3) good cyto/hemocompatibility. An interesting size dependent cytotoxicity behavior was observed in HeLa and NIH/3T3 cell lines: smallest particles are internalized significantly more by both of the cell lines, yet showed almost no significant cytotoxicity in HeLa between 10 and 25μg/mL Ag concentration but were most toxic in NIH/3T3 cells. Cell internalization and hence the cytotoxicity enhanced when cells were incubated with the hybrid nanoparticles under magnetic field, especially with the hybrid nanoparticles containing larger amounts of SPION in the hybrid composition. These results prove them as effective optical imaging agents and magnetic delivery vehicles. Combined with the known advantages of SPIONs as a contrast agent in MRI, these particles are a step forward for new theranostics for multimode imaging and magnetic targeting.
authors.affiliation: Materials Science and Engineering, Koc University, Istanbul 34450, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Hocaoglu
authors.affiliation: Department of Molecular Biology and Genetics, Koc University, Istanbul 34450, Turkey.
authors.forename: Didar
authors.initials: D
authors.lastname: Asik
authors.affiliation: Department of Chemistry, Koc University, Istanbul 34450, Turkey.
authors.forename: Gulen
authors.initials: G
authors.lastname: Ulusoy
authors.affiliation: Center Interfacultaire des Biomateriaux, University of Liege, Liege B 4000, Belgium.
authors.forename: Christian
authors.initials: C
authors.lastname: Grandfils
authors.affiliation: Institute of Health and Consumer Protection, European Commission Joint Research Center, Ispra 21027, Italy.
authors.forename: Isaac
authors.initials: I
authors.lastname: Ojea-Jimenez
authors.affiliation: Institute of Health and Consumer Protection, European Commission Joint Research Center, Ispra 21027, Italy.
authors.forename: François
authors.initials: F
authors.lastname: Rossi
authors.affiliation: Materials Science and Engineering, Koc University, Istanbul 34450, Turkey; Department of Physics, Koc University, Istanbul 34450, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Kiraz
authors.affiliation: Department of Physics, Gebze Technical University, Gebze, Turkey.
authors.forename: Nurcan
authors.initials: N
authors.lastname: Doğan
authors.affiliation: Materials Science and Engineering, Koc University, Istanbul 34450, Turkey; Department of Chemistry, Koc University, Istanbul 34450, Turkey. Electronic address: fyagci@ku.edu.tr.
authors.forename: Havva Yagci
authors.initials: HY
authors.lastname: Acar
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D049449:Luminescence; D008280:Magnetics; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D051379:Mice; D041681:NIH 3T3 Cells; D000068936:Theranostic Nanomedicine
chemical_list: D001672:Biocompatible Materials
keywords: Hybrid nanoparticles; Magnetic nanoparticle; Near-infrared; Quantum dot; Silver sulfide