title: Cloning, expression and purification of d-tagatose 3-epimerase gene from Escherichia coli JM109.
abstract: An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatography, highly purified and stable DTE protein was produced. The molecular weight of the DTE protein was estimated to be 29.8kDa. The latest 83 DTE sequences from public database were selected and analyzed by molecular clustering, multi-sequence alignment. DTEs were roughly divided into five categories.
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China. Electronic address: realren@sina.com.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: He
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
authors.forename: Le
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Yu
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Jia
authors.affiliation: School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
mesh_terms: D003001:Cloning, Molecular; D016000:Cluster Analysis; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006601:Hexoses / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D006601:Hexoses; D011994:Recombinant Proteins; C030192:tagatose
keywords: Cloning; Escherichia coli; Expression; Purification; d-Tagatose 3-epimerase