title: High-Throughput DNA Sequencing Analysis of Antibody Repertoires.
abstract: New high-throughput DNA sequencing (HTS) technologies developed in the past decade have begun to be applied to the study of the complex gene rearrangements that encode human antibodies. This article first reviews the genetic features of Ig loci and the HTS technologies that have been applied to human repertoire studies, then discusses key choices for experimental design and data analysis in these experiments and the insights gained in immunological and infectious disease studies with the use of these approaches.
authors.affiliation: Department of Pathology, Stanford University, Stanford, CA 94305.
authors.forename: Scott D
authors.initials: SD
authors.lastname: Boyd
authors.affiliation: Department of Pathology, Stanford University, Stanford, CA 94305.
authors.forename: Shilpa A
authors.initials: SA
authors.lastname: Joshi
mesh_terms: D000906:Antibodies / Q000235:genetics*; D015321:Gene Rearrangement; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D017422:Sequence Analysis, DNA
chemical_list: D000906:Antibodies