title: Identification and Purification of the CPD Photolyase in Vibrio parahaemolyticus RIMD2210633.
abstract: Photoreactivation is an error-free mechanism of DNA repair, utilized by prokaryotes and most eukaryotes and is catalyzed by specific enzymes called DNA photolyases. Photoreactivation has been reported in Vibrio parahaemolyticus WP28; however, information on photolyases in V. parahaemolyticus (V.p) strains has not been reported. This study examined the photoreactivation in V.p RIMD2210633. The photolyase responsible for repairing cyclobutane pyrimidine dimer (CPD) in DNA was identified, and the corresponding gene was determined as VPA1471. The protein was overexpressed in Escherichia coli and was purified for functional assessment in vitro. The mRNA level and protein expression level of this gene increased after ultraviolet A (UVA) illumination following ultraviolet C (UVC) irradiation. In vitro experiments confirmed that the protein encoded by VPA1471 could reduce the quantity of CPD in DNA. We designated the corresponding gene and protein of VPA1471 phr and Phr, respectively, although the function of two other photolyase/cryptochrome family members, VPA0203 and VPA0204, remains unclear. UV (ultraviolet) irradiation experiments suggest that these two genes possess some photorepairing ability. Therefore, we hypothesize that VPA0203 and VPA0204 encode (6-4) photolyase in V. parahaemolyticus RIMD2210633.
authors.affiliation: Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang, China.|Department of Preventive Environment and Nutrition, Institute of Health Bioscience, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Zehong
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang, China.
authors.forename: Gaojian
authors.initials: G
authors.lastname: Lian
authors.affiliation: Department of Preventive Environment and Nutrition, Institute of Health Bioscience, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Mawatari
authors.affiliation: Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Tang
authors.affiliation: Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang, China.
authors.forename: Shuya
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Preventive Environment and Nutrition, Institute of Health Bioscience, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Shimohata
authors.affiliation: Department of Microbiology and Immunology, University of South China, Hengyang, China.
authors.forename: Yimou
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Yin
authors.affiliation: Department of Preventive Environment and Nutrition, Institute of Health Bioscience, University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
mesh_terms: D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000302:isolation & purification*; D011740:Pyrimidine Dimers / Q000302:isolation & purification; D014736:Vibrio parahaemolyticus / Q000201:enzymology*
chemical_list: D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase