title: In vitro and in vivo evaluation of PEGylated nanoparticles of bendamustine for treatment of lung cancer.
abstract: The purpose of this study was to develop PEGylated nanoparticles of bendamustine (BM) to improve therapeutic efficiency of drug and reduce the side-effects. The nanoparticles were prepared by a modified diffusion-emulsification method. The particle size and zeta potential of optimized BM-loaded PEGylated NPs were found to be 256 nm and -29.1 mV. The in vitro release showed biphasic behavior, with initial burst release followed by slow sustained delivery. The anti-tumor activity was determined using the A- 549 cell line, by the MTT assay. The stability study revealed that the nanoparticles prepared were stable for 3 months at both 25°C and 4°C.
authors.affiliation: a Columbia Institute of Pharmacy , Raipur, Tekari, Chhattisgarh , India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Dubey
authors.affiliation: b University Institute of Pharmacy, Pandit Ravishankar Shukla University , Raipur, Chhattisgarh , India.
authors.forename: Bina
authors.initials: B
authors.lastname: Gidwani
authors.affiliation: a Columbia Institute of Pharmacy , Raipur, Tekari, Chhattisgarh , India.
authors.forename: Ravindra
authors.initials: R
authors.lastname: Pandey
authors.affiliation: a Columbia Institute of Pharmacy , Raipur, Tekari, Chhattisgarh , India.
authors.forename: Shiv Shankar
authors.initials: SS
authors.lastname: Shukla
mesh_terms: D000818:Animals; D000069461:Bendamustine Hydrochloride / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: D011092:Polyethylene Glycols; D000069461:Bendamustine Hydrochloride; C000595209:polyethylene glycol 1000
keywords: PEGylation; bendamustine; lung cancer; nanoparticles