title: Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar.
abstract: Trees adjust their growth following forced changes in orientation to re-establish a vertical position. In angiosperms, this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formation of differential wood types through a quantitative proteomic and phosphoproteomic analysis on poplar subjected to a gravitropic stimulus. We identified and quantified 675 phosphopeptides, corresponding to 468 phosphoproteins, and 3 763 nonphosphorylated peptides, corresponding to 1 155 proteins, in the differentiating xylem of straight-growing trees (control) and trees subjected to a gravitational stimulus during 8 weeks. About 1% of the peptides were specific to a wood type (straight, opposite, or tension wood). Proteins quantified in more than one type of wood were more numerous: a mixed linear model showed 389 phosphopeptides and 556 proteins to differ in abundance between tension wood and opposite wood. Twenty-one percent of the phosphoproteins identified here were described in their phosphorylated form for the first time. Our analyses revealed remarkable developmental molecular plasticity, with wood type-specific phosphorylation events, and highlighted the involvement of different proteins in the biosynthesis of cell wall components during the formation of the three types of wood.
authors.affiliation: †INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.|‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Mauriat
authors.affiliation: §INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Leplé
authors.affiliation: ⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.affiliation: †INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.|‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bartholomé
authors.affiliation: ⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Negroni
authors.affiliation: §INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Richet
authors.affiliation: †INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.|‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Lalanne
authors.affiliation: ⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneu
authors.affiliation: ¶INRA, UMR 547 PIAF, 234 Avenue du Brézet, F-63100 Clermont-Ferrand, France.|∥Clermont Université, Université Blaise Pascal, UMR 547 PIAF, F-63100 Clermont-Ferrand, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Coutand
authors.affiliation: †INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.|‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Plomion
mesh_terms: D000595:Amino Acid Sequence; D016000:Cluster Analysis; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006112:Gravitation; D018522:Gravitropism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010748:Phosphopeptides / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics; D014934:Wood / Q000235:genetics / Q000378:metabolism*; D052584:Xylem / Q000235:genetics / Q000378:metabolism
chemical_list: D010455:Peptides; D010748:Phosphopeptides; D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome
keywords: cell wall; phosphoproteomics; poplar; proteomics; tension wood; wood formation