title: Relationship between genetic polymorphisms of DNA ligase 1 and non-small cell lung cancer susceptibility and radiosensitivity.
abstract: The aim of this study was to examine the relationship between genetic polymorphisms in DNA ligase 1 (LIG1) and non-small cell lung cancer (NSCLC) susceptibility and radiosensitivity in a Chinese population. This was a case-control study that included 352 NSCLC patients and 448 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism analysis was conducted to detect HaeIII polymorphisms in exon 6 of the LIG1 gene in this popula-tion. This information was used to observe the effects of radiation in pa-tients with different genotypes in order to determine the genotypes as-sociated with radiosensitivity. The CC genotype and C allele frequency were significantly higher in the NSCLC group than in the control group (P = 0.012 and P = 0.023, respectively). The relative risk of experienc-ing NSCLC was 2.55 [95% confidence interval (CI), 1.12-3.98] for CC homozygous patients and 0.87 (95%CI, 0.46-1.88) for AA homozygous patients. Analysis of LIG1 genetic polymorphisms and radiosensitiv-ity of NSCLC patients showed that AA homozygous patients were sig-nificantly more radiosensitive than the control group (AA vs AC, P = 0.014; AA vs CC, P < 0.001; AC vs CC, P = 0.023). Therefore, the LIG1 CC genotype was associated with susceptibility to NSCLC, and the AA genotype demonstrated increased radiosensitivity compared to the AC and CC genotypes.
authors.affiliation: The Fourth Clinical Medical College of Nanjing Medical University,Nanjing, Jiangsu, China.
authors.forename: H
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Radiation Oncology, The Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, xiahe_th@163.com.
authors.forename: X
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Radiation Oncology, The Affiliated Jiangsu Cancer Hospital of Nanjing Medical University.
authors.forename: L
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Radiation Oncology, The Affiliated Jiangsu Cancer Hospital of Nanjing Medical University.
authors.forename: W J
authors.initials: WJ
authors.lastname: Guo
authors.affiliation: The Fourth Clinical Medical College of Nanjing Medical University,Nanjing, Jiangsu, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Xia
authors.affiliation: Department of Radiation Oncology, The First People's Hospital of Xinyi, Xinyi Jiangsu, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Jiang
mesh_terms: D000368:Aged; D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D016022:Case-Control Studies; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics*; D005091:Exons; D005260:Female; D005720:Gamma Rays / Q000627:therapeutic use*; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006720:Homozygote; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011836:Radiation Tolerance
chemical_list: C000607892:LIG1 protein, human; D011088:DNA Ligases; D000072481:DNA Ligase ATP