title: Effect of serum-derived albumin scaffold and canine adipose tissue-derived mesenchymal stem cells on osteogenesis in canine segmental bone defect model.
abstract: Composite biological and synthetic grafts with progenitor cells offer an alternative approach to auto- or allografts for fracture repair. This study was conducted to evaluate osteogenesis of autologous serum-derived albumin (ASA) scaffolds seeded with canine adipose tissue-derived mesenchymal stem cells (Ad-MSCs) in a canine segmental bone defect model. ASA scaffold was prepared with canine serum using cross-linking and freeze-drying procedures. Beta-tricalcium phosphate (β-TCP) was mixed at the cross-linking stage. Ad-MSCs were seeded into the scaffold and incubated for one day before implantation. After 16 weeks, the grafts were harvested for histological analysis. The dogs were divided into five groups: control, ASA scaffolds with and without Ad-MSCs, and ASA scaffolds including β-TCP with and without Ad-MSCs. ASA scaffolds with Ad-MSCs had a significantly larger area of increased opacity at the proximal and distal host cortex-implant interfaces in radiographs 16 weeks after implantation compared to the groups with β-TCP (p < 0.05). Histomorphometric analysis showed that ASA scaffolds with Ad-MSCs had significantly greater new bone formation than other groups (p < 0.05). These results suggest that Ad-MSCs seeded into ASA scaffolds enhanced osteogenesis in the bone defect model, but that β-TCP in the ASA scaffold might prevent penetration of the cells required for bone healing.
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Yoon
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Byung-Jae
authors.initials: BJ
authors.lastname: Kang
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.|BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Yongsun
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.|BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Daeun
authors.initials: D
authors.lastname: Rhew
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Wan Hee
authors.initials: WH
authors.lastname: Kim
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.|BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Oh-Kyeong
authors.initials: OK
authors.lastname: Kweon
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000648:ultrastructure; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004285:Dogs; D050723:Fractures, Bone / Q000628:therapy*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology*; D012709:Serum Albumin / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D012709:Serum Albumin; C485817:beta-tricalcium phosphate
keywords: adipose tissue-derived mesenchymal stem cells; bone defect; serum-derived albumin scaffold