title: Monitoring of Water Spectral Pattern Reveals Differences in Probiotics Growth When Used for Rapid Bacteria Selection.
abstract: Development of efficient screening method coupled with cell functionality evaluation is highly needed in contemporary microbiology. The presented novel concept and fast non-destructive method brings in to play the water spectral pattern of the solution as a molecular fingerprint of the cell culture system. To elucidate the concept, NIR spectroscopy with Aquaphotomics were applied to monitor the growth of sixteen Lactobacillus bulgaricus one Lactobacillus pentosus and one Lactobacillus gasseri bacteria strains. Their growth rate, maximal optical density, low pH and bile tolerances were measured and further used as a reference data for analysis of the simultaneously acquired spectral data. The acquired spectral data in the region of 1100-1850nm was subjected to various multivariate data analyses - PCA, OPLS-DA, PLSR. The results showed high accuracy of bacteria strains classification according to their probiotic strength. Most informative spectral fingerprints covered the first overtone of water, emphasizing the relation of water molecular system to cell functionality.
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan; University of Food Technologies, Department of Microbiology, 26 "Maritza" Blvd., 4002 Plovdiv, Bulgaria.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Slavchev
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan; Corvinus University of Budapest, Faculty of Food Science, Department of Physics and Control, 14-16 Somlói str., Budapest 1118, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kovacs
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Koshiba
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Airi
authors.initials: A
authors.lastname: Nagai
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan; Kaposvár University, Faculty of Agricultural and Environmental Sciences, Institute of Food and Agricultural Product Qualification, 40 Guba Sándor str., Kaposvár 7400, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Bázár
authors.affiliation: University of Food Technologies, Department Biotechnology, 26 "Maritza" Blvd., 4002 Plovdiv, Bulgaria.
authors.forename: Albert
authors.initials: A
authors.lastname: Krastanov
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: Kobe University, Graduate School of Agricultural Science, Biomeasurement Technology Laboratory, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Roumiana
authors.initials: R
authors.lastname: Tsenkova
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D025341:Principal Component Analysis; D019936:Probiotics / Q000494:pharmacology*; D019265:Spectroscopy, Near-Infrared; D014867:Water / Q000737:chemistry*
chemical_list: D001647:Bile Acids and Salts; D014867:Water