title: The effect of alpha lipoic acid on rat kidneys in methotrexate induced oxidative injury.
abstract: OBJECTIVE
  The purpose of this study is to determine the antioxidant and anti-inflammatory effects of alpha lipoic acid (ALA) on methotrexate (MTX) induced kidney injury in rats.
  
  
  METHODS
  Thirty-two rats were equally divided into four groups; control, ALA, MTX and MTX with ALA groups. A single dose of MTX (20 mg/kg) was administered to make kidney injury to groups 3 and 4, intraperitoneally. The ALA was administered intraperitonealy in groups 2 and 4 and the other groups received saline injection for five days. On the sixth day the blood samples and kidney tissues were obtained for the measurement of TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels and histological examination.
  
  
  RESULTS
  Administration of MTX caused a decrease in tissue GSH, and Na+, K+-ATPase activity significantly. A significant increase in tissue MDA and MPO activities were also seen. The pro-inflammatory cytokines (TNF-α, IL-β) were increased in the MTX group significantly. ALA treatment reversed all biochemical indices as well as histopathological alterations induced by MTX administration.
  
  
  CONCLUSIONS
  MTX made oxidative damage on kidneys of rat and it was partially prevented by anti-inflammatory and antioxidant effects of ALA treatment.
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey. tugrul-cakir@hotmail.com.
authors.forename: T
authors.initials: T
authors.lastname: Çakır
authors.forename: C
authors.initials: C
authors.lastname: Polat
authors.forename: A
authors.initials: A
authors.lastname: Baştürk
authors.forename: M
authors.initials: M
authors.lastname: Gül
authors.forename: A
authors.initials: A
authors.lastname: Aslaner
authors.forename: H
authors.initials: H
authors.lastname: Durgut
authors.forename: A Ö
authors.initials: AÖ
authors.lastname: Şehirli
authors.forename: A
authors.initials: A
authors.lastname: Aykaç
authors.forename: L
authors.initials: L
authors.lastname: Bahar
authors.forename: M Z
authors.initials: MZ
authors.lastname: Sabuncuoglu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D005260:Female; D005978:Glutathione; D053583:Interleukin-1beta / Q000097:blood; D007668:Kidney / Q000187:drug effects*; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D008315:Malondialdehyde; D008727:Methotrexate / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D008063:Thioctic Acid; D009195:Peroxidase; D005978:Glutathione; D008727:Methotrexate