title: Therapeutic Effectiveness in the Treatment of Experimental Bacterial Keratitis with Ion-activated Mucoadhesive Hydrogel.
abstract: OBJECTIVE
  To investigate the therapeutic effectiveness of ion-activated mucoadhesive hydrogel system in the treatment of experimental bacterial keratitis.
  
  
  METHODS
  Mucoadhesive systems were prepared using gellan or sodium alginate alone and combined with sodium carboxymethylcellulose (NaCMC) to enhance the gel bioadhesion properties. The in vivo antimicrobial efficacy of selected mucoadhesive systems was studied in an experiment on bacterial keratitis in rabbit's eyes and compared with that of the marketed conventional eyedrops.
  
  
  RESULTS
  Ocular tolerance was studied in the eye of albino rabbits and tested formulations were non-irritant with no sign of inflammation. Better improvement in experimental bacterial keratitis in rabbit eyes was observed in animals treated with mucoadhesive hydrogel formulation (GG5 and GS5) compared with marketed drug solution.
  
  
  CONCLUSIONS
  The developed system is a viable alternative to conventional eyedrops of GTN due to its ability to enhance bioavailability through its longer precorneal residence time.
authors.affiliation: a Department of Pharmaceutics , Institute of Technology, Banaras Hindu University , Varanasi , Uttar Pradesh , India .|b Institute of Pharmaceutical Sciences, Guru Ghasdas Vishwavidyalaya, Koni , Bilaspur , Chhattisgarh , India , and.
authors.forename: Karthikeyan
authors.initials: K
authors.lastname: Kesavan
authors.affiliation: c Department of Ophthalmology , Institute of Medical Science, Banaras Hindu University , Varanasi , Uttar Pradesh , India.
authors.forename: Shri
authors.initials: S
authors.lastname: Kant
authors.affiliation: a Department of Pharmaceutics , Institute of Technology, Banaras Hindu University , Varanasi , Uttar Pradesh , India .|c Department of Ophthalmology , Institute of Medical Science, Banaras Hindu University , Varanasi , Uttar Pradesh , India.
authors.forename: Jayanta Kumar
authors.initials: JK
authors.lastname: Pandit
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001682:Biological Availability; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D003320:Corneal Ulcer / Q000188:drug therapy* / Q000382:microbiology; D004195:Disease Models, Animal; D004337:Drug Carriers; D015818:Eye Infections, Bacterial / Q000188:drug therapy* / Q000382:microbiology; D024841:Fluoroquinolones / Q000008:administration & dosage*; D000077734:Gatifloxacin; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000008:administration & dosage* / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects; D016896:Treatment Outcome
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D024841:Fluoroquinolones; D006603:Hexuronic Acids; D020100:Hydrogels; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D020723:Glucuronic Acid; D002266:Carboxymethylcellulose Sodium; D000077734:Gatifloxacin
keywords: Bacterial keratitis; gatifloxacin; in vivo antibacterial; ion-activated hydrogel; mucoadhesive; rabbit eyes