title: Genotypes of hepatitis B virus identified in patients tested prior to endoscopy from a Teaching Hospital in the Central Province of Sri Lanka.
abstract: The present study was carried out to identify the hepatitis B virus (HBV) genotypes in six patients attending the surgical clinic who were positive for hepatitis B surface antigen. DNA was extracted from the serum of patients and subjected to a modified nested PCR to detect a 585 bp region within the S gene of the HBV genome. Positive PCR products were purified and sequenced via a cycle sequencing method. The sequence data were analyzed with reference sequences in the HepSEQ database to identify the particular HBV genotype of the samples. Nested PCR for the S gene of the HBV genome was positive in 2 out of 6 samples. The genotyping and sequence analysis of the PCR products showed HBV genotype A with a homology of 98% to the reference sequences in the HepSeq database.
authors.forename: D G D
authors.initials: DG
authors.lastname: Jayasuriya
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Peradeniya, Sri Lanka. faseeha.noordeen12@gmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Noordeen
authors.forename: F N N
authors.initials: FN
authors.lastname: Pitchai
mesh_terms: D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D004724:Endoscopy; D016679:Genome, Viral / Q000235:genetics; D005838:Genotype; D006509:Hepatitis B / Q000097:blood / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006784:Hospitals, Teaching; D006801:Humans; D016133:Polymerase Chain Reaction; D011300:Preoperative Care; D011498:Protein Precursors / Q000097:blood / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013188:Sri Lanka; D014759:Viral Envelope Proteins / Q000097:blood / Q000235:genetics
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen