title: Gene Silencing by RNAi in Mammalian Cells.
abstract: This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.
authors.affiliation: Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: Frida
authors.initials: F
authors.lastname: Ponthan
authors.affiliation: Advanced Medical & Dental Institute, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
authors.forename: Narazah Mohd
authors.initials: NM
authors.lastname: Yusoff
authors.affiliation: Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: Natalia Martinez
authors.initials: NM
authors.lastname: Soria
authors.affiliation: Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: Olaf
authors.initials: O
authors.lastname: Heidenreich
authors.affiliation: Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: Kelly
authors.initials: K
authors.lastname: Coffey
mesh_terms: D000818:Animals; D002460:Cell Line; D020868:Gene Silencing; D006801:Humans; D008322:Mammals; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods; D014162:Transfection / Q000379:methods
chemical_list: D034741:RNA, Small Interfering
keywords: RNA interference; electroporation; lentiviral transduction; shRNA; siRNA; transfection