title: Clinical features and mutation status of EGFR, KRAS, BRAF, EML4-ALK and ROS1 between surgical resection samples and non surgical resection samples in lung cancer.
abstract: BACKGROUND
  Target therapy is the first-line treatment in lung cancer. The testing of driver gene mutations is crucial for decision of treatment. Many lung cancer patients are in advanced grade, and lose the chance of operation.
  
  
  METHODS
  The tissue used to perform mutation testing is only from biopsy. In order to analysis the difference between surgical resection samples (SRSs) and non-surgical resection samples (NSRSs), 1,357 surgical tissues and 145 biopsy samples histopathologically diagnosed with lung cancer were collected to detect the mutation status of EGFR, KRAS, BRAF, EML4-ALK and ROS1 in this study.
  
  
  RESULTS
  There were no significant differences of age, gender, and histological type between the two group patients we collected; however, the significant difference was present in grade. More early stage patients were in the surgical group, but more advanced stage lung cancer patients were in non surgical group. In the mutation analysis, we also found no significant differences in all driver genes we detected between the two groups.
  
  
  CONCLUSIONS
  Both surgical resection samples and biopsy samples could be used to perform the testing the driver gene mutation.
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai pulmonary hospital, Tongji University School of Medicine, Shanghai 200433, China ; 2 Department of Thoracic Surgery, Shanghai Changzheng hospital Affiliated to Shanghai Second Military University, Shanghai 200061, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai pulmonary hospital, Tongji University School of Medicine, Shanghai 200433, China ; 2 Department of Thoracic Surgery, Shanghai Changzheng hospital Affiliated to Shanghai Second Military University, Shanghai 200061, China.
authors.forename: Jichen
authors.initials: J
authors.lastname: Qu
authors.affiliation: 1 Department of Thoracic Surgery, Shanghai pulmonary hospital, Tongji University School of Medicine, Shanghai 200433, China ; 2 Department of Thoracic Surgery, Shanghai Changzheng hospital Affiliated to Shanghai Second Military University, Shanghai 200061, China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Xu
keywords: Surgical resection sample (SRS); driver gene mutation; lung cancer; non-surgical resection sample (NSRS)