title: Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed.
abstract: Fluorescence resonance energy transfer-based assay showed that archaeal ribonuclease P (RNase P) proteins significantly promoted DNA annealing and strand displacement. Moreover, we found that archaeal RNase P proteins could discriminate nucleotide exchanges in DNA chains via their activity accelerating DNA strand displacement, suggesting that they have potential for biotechnological application to genetic diagnosis.
authors.affiliation: a Laboratory of Structural Biology , Graduate School of Systems Life Sciences , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Miyanoshita
authors.affiliation: a Laboratory of Structural Biology , Graduate School of Systems Life Sciences , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.|b Laboratory of Biochemistry, Faculty of Agriculture, Department of Bioscience and Biotechnology, Graduate School , Kyushu University , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.affiliation: a Laboratory of Structural Biology , Graduate School of Systems Life Sciences , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.|b Laboratory of Biochemistry, Faculty of Agriculture, Department of Bioscience and Biotechnology, Graduate School , Kyushu University , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kakuta
authors.affiliation: a Laboratory of Structural Biology , Graduate School of Systems Life Sciences , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.|b Laboratory of Biochemistry, Faculty of Agriculture, Department of Bioscience and Biotechnology, Graduate School , Kyushu University , Hakozaki 6-10-1, Fukuoka 812-8581 , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
mesh_terms: D001709:Biotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D043902:Pyrococcus horikoshii / Q000201:enzymology*; D043262:Ribonuclease P / Q000378:metabolism*
chemical_list: D043262:Ribonuclease P
keywords: RNA annealing; RNase P proteins; fluorescence resonance energy transfer; strand displacement