title: Effect of eccentric contraction on satellite cell activation in human vastus lateralis muscle.
abstract: We compared the time-course of satellite cell (SC) activation between eccentric and concentric contractions in the vastus lateralis (VL) muscle after step exercise. Young adults participated in a 30-min step up/down exercise which mainly involved concentric contractions with the right VL muscle and eccentric contractions with the left VL muscle. The concentric and eccentric contraction phases of the VL muscles were identified by changes in the electromyogram (EMG) and knee joint angle. Biopsy samples were taken from both VL muscles at three time periods: before the exercise and 2 and 5 days after the exercise. We found that the numbers of SCs were significantly increased in the type IIa fibers of the left VL at 2 and 5 days after the exercise. The expression of both hepatocyte growth factor (HGF) and myogenic differentiation 1 (MyoD) mRNA had significantly increased in the left VL at 2 and 5 days after the exercise and in the right VL at 5 days after the exercise. The expression of transient receptor potential canonical (TRPC) 1 mRNA also increased in the left VL at 2 days after exercise. These results indicate that eccentric contraction can effectively activate SC proliferation for up to 5 days after exercise. Similar changes in HGF, MyoD and TRPC1 mRNA expression suggest that HGF/c-Met signal activation through cation influx has a major impact on skeletal muscle SC activation in response to eccentric exercise.
authors.affiliation: Department of Biological Sciences, Graduate School of Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Imaoka
authors.forename: Minako
authors.initials: M
authors.lastname: Kawai
authors.forename: Futoshi
authors.initials: F
authors.lastname: Mori
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyata
mesh_terms: D001696:Biomechanical Phenomena; D001706:Biopsy; D049109:Cell Proliferation; D004576:Electromyography; D005260:Female; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007719:Knee Joint / Q000502:physiology; D008297:Male; D009119:Muscle Contraction; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D052097:Quadriceps Muscle / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016059:Range of Motion, Articular; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C066855:HGF protein, human; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D012333:RNA, Messenger; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D017228:Hepatocyte Growth Factor