title: Sodium Hydroxide Pinpoint Pressing Permeation Method for the Animal Modeling of Sick Sinus Syndrome.
abstract: Sodium hydroxide pinpoint pressing permeation (SHPPP) was investigated in order to build a rat model of sick sinus syndrome (SSS), which is easy to operate and control the degree of damage, with fewer complications and applicable for large and small animals.Thirty healthy Wistar rats (15 males and 15 females, weighing 250-350 g) were randomly divided into 3 groups, namely a formaldehyde thoracotomy wet compressing group (FTWC), formaldehyde pinpoint pressing permeation group (FPPP) group, and SHPPP group. The number of surviving rats, heart rate (HR), sinoatrial node recovery time (SNRT), corrected SNRT (CSNRT), and sinoatrial conduction time (SACT) were recorded 3 days, one week, and two weeks after modeling.The achievement ratio of modeling was 10% in the FTWC group, 40% in the FPPP group, and 70% in the SHPPP group, and the differences were statistically significant (χ(2) = 7.250, P = 0.007). Meanwhile, the HR was reduced by about 37% in these 3 groups 3 days after modeling, while the reduction was maintained only in SHPPP (P > 0.05) and the HR was re-elevated in the FTWC and FPPP groups 2 weeks after modeling (P < 0.05). Additionally, the SNRT, cS-NRT, and SACT were significantly prolonged compared with pre-modeling in all 3 groups (P < 0.01).SHPPP was the best method with which to build an SSS model with stable and lasting low HR and high success rate of modeling, which might be helpful for further studies on the SSS mechanisms and drugs.
authors.affiliation: Third Ward, Department of Cardiovascular, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine.
authors.forename: Naizhi
authors.initials: N
authors.lastname: Geng
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.forename: Cailiang
authors.initials: C
authors.lastname: Peng
authors.forename: Huaiping
authors.initials: H
authors.lastname: Wang
authors.forename: Shuoxin
authors.initials: S
authors.lastname: Zhang
authors.forename: Tianyu
authors.initials: T
authors.lastname: Chen
authors.forename: Lixia
authors.initials: L
authors.lastname: Liu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wu
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D022062:Electrophysiologic Techniques, Cardiac / Q000379:methods; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012804:Sick Sinus Syndrome / Q000209:etiology / Q000503:physiopathology; D012849:Sinoatrial Node / Q000187:drug effects / Q000503:physiopathology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013908:Thoracotomy / Q000379:methods; D013997:Time Factors
chemical_list: D005557:Formaldehyde; D012972:Sodium Hydroxide