title: Antitumor Properties of the Essential Oil From the Leaves of Duguetia gardneriana.
abstract: Duguetia gardneriana, popularly known in the Brazilian northeast as "jaquinha", is a species belonging to the family Annonaceae. The aim of this work was to assess the chemical composition and antitumor properties of the essential oil from the leaves of D. gardneriana in experimental models. The chemical composition of the essential oil was analyzed via gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. In vitro cytotoxic activity was determined in cultured tumor cells, and in vivo antitumor activity was assessed in B16-F10-bearing mice. The identified compounds were β-bisabolene (80.99%), elemicin (8.04%), germacrene D (4.15%), and cyperene (2.82%). The essential oil exhibited a cytotoxic effect, with IC50 values of 16.89, 19.16, 13.08, and 19.33 µg/mL being obtained for B16-F10, HepG2, HL-60, and K562 cell lines, respectively. On the other hand, β-bisabolene was inactive in all of the tested tumor cell lines (showing IC50 values greater than 25 µg/mL). The in vivo analysis revealed tumor growth inhibition rates of 5.37-37.52% at doses of 40 and 80 mg/kg/day, respectively. Herein, the essential oil from the leaves of D. gardneriana presented β-bisabolene as the major constituent and showed cytotoxic and antitumor potential.
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Ana Carolina B C
authors.initials: AC
authors.lastname: Rodrigues
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Larissa M
authors.initials: LM
authors.lastname: Bomfim
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Sara P
authors.initials: SP
authors.lastname: Neves
authors.affiliation: Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
authors.forename: Leociley R A
authors.initials: LR
authors.lastname: Menezes
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Rosane B
authors.initials: RB
authors.lastname: Dias
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Milena B P
authors.initials: MB
authors.lastname: Soares
authors.affiliation: Department of Biology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
authors.forename: Ana Paula N
authors.initials: AP
authors.lastname: Prata
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Clarissa A Gurgel
authors.initials: CA
authors.lastname: Rocha
authors.affiliation: Department of Chemistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
authors.forename: Emmanoel V
authors.initials: EV
authors.lastname: Costa
authors.affiliation: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (Fiocruz), Salvador, Bahia, Brazil.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Bezerra
mesh_terms: D000328:Adult; D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology; D045788:Sesquiterpenes, Germacrane / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C000606812:cyperene; D011748:Pyrogallol; C002135:elemicin; C531191:beta-bisabolene; C027259:germacrene D