title: MARCC (Matrix-Assisted Reader Chromatin Capture): An Antibody-Free Method to Enrich and Analyze Combinatorial Nucleosome Modifications.
abstract: Combinatorial patterns of histone modifications are key indicators of different chromatin states. Most of the current approaches rely on the usage of antibodies to analyze combinatorial histone modifications. Here we detail an antibody-free method named MARCC (Matrix-Assisted Reader Chromatin Capture) to enrich combinatorial histone modifications. The combinatorial patterns are enriched on native nucleosomes extracted from cultured mammalian cells and prepared by micrococcal nuclease digestion. Such enrichment is achieved by recombinant chromatin-interacting protein modules, or so-called reader domains, which can bind in a combinatorial modification-dependent manner. The enriched chromatin can be quantified by immunoblotting or mass spectrometry for the co-existence of histone modifications, while the associated DNA content can be analyzed by qPCR or next-generation sequencing. Altogether, MARCC provides a reproducible, efficient and customizable solution to enrich and analyze combinatorial histone modifications.
authors.affiliation: Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin.|Wisconsin Institutes for Discovery, University of Wisconsin, Madison, Wisconsin.
authors.forename: Zhangli
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin.|Wisconsin Institutes for Discovery, University of Wisconsin, Madison, Wisconsin.
authors.forename: John M
authors.initials: JM
authors.lastname: Denu
mesh_terms: D002843:Chromatin / Q000737:chemistry* / Q000302:isolation & purification*; D003584:Cytological Techniques / Q000379:methods*; D042421:Histone Code; D008967:Molecular Biology / Q000379:methods*; D009707:Nucleosomes / Q000737:chemistry*
chemical_list: D002843:Chromatin; D009707:Nucleosomes
keywords: MARCC; PTM; affinity enrichment; antibody-free; chromatin; histone; reader domain