title: Supplementation of Citrus maxima Peel Powder Prevented Oxidative Stress, Fibrosis, and Hepatic Damage in Carbon Tetrachloride (CCl4) Treated Rats.
abstract: Citrus maxima peel is rich in natural phenolic compounds and has a long use in the traditional medicine. HPLC-DAD analysis on Citrus maxima peel powder exhibited the presence of various phenolic compounds such as caffeic acid and (-)-epicatechin. To determine the plausible hepatoprotective activity of Citrus maxima peel powder, we used carbon tetrachloride (CCl4) treated rat model. Liver damage in rats was confirmed by measuring the AST, ALT, and ALP enzyme activities. In addition, lipid peroxidation products (MDA), nitric oxide, advanced protein oxidation products level (APOP), and catalase activities were also analyzed along with the histological profiling for the inflammatory cell infiltration, collagen, and iron deposition in liver. Dietary supplementation of Citrus maxima peel powder exhibited significant reduction of serum AST, ALT, and ALP activities in carbon tetrachloride treated rats. Moreover, Citrus maxima peel powder also showed a significant reduction of the oxidative stress markers (MDA, NO, and APOP level) and restored the catalase activity in CCl4 treated rats. Histological examination of the liver section revealed reduced inflammatory cells infiltration, collagen, and iron deposition in CCl4 treated rats. The results from this study demonstrated that Citrus maxima peel powder produced significant hepatoprotective action in CCl4 administered rats.
authors.affiliation: Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
authors.forename: Mohammed Riaz Hasan
authors.initials: MR
authors.lastname: Chowdhury
authors.affiliation: Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
authors.forename: Md Abu Taher
authors.initials: MA
authors.lastname: Sagor
authors.affiliation: Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
authors.forename: Nabila
authors.initials: N
authors.lastname: Tabassum
authors.affiliation: Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
authors.forename: Md Abdullah
authors.initials: MA
authors.lastname: Potol
authors.affiliation: BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh.
authors.forename: Hemayet
authors.identifier: 0000-0001-8759-9279
authors.initials: H
authors.lastname: Hossain
authors.affiliation: Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
authors.forename: Md Ashraful
authors.identifier: 0000-0001-7596-5868
authors.initials: MA
authors.lastname: Alam