title: PP025. Measurement of the activity of the circulating and intrarenal renin-angiotensin system (iRAS) in pregnant and non-pregnant subjects.
abstract: OBJECTIVE
  To see if urinary angiotensinogen (uAGT)/creatinine and other urinary components of the RAS could be used to detect renal disease in pregnancy, as renal disease predisposes to preeclampsia.
  
  
  METHODS
  Plasma and urinary prorenin, ACE and AGT (iRAS) were measured by ELISA. Urinary active renin levels were measured enzymatically (9 males, 10 non pregnant, 61 Australian Indigenous pregnant women).
  
  
  RESULTS
  No relationships between plasma RAS and iRAS were found. In non-pregnant females plasma AGT levels were inversely related to protein and albumin/creatinine (r=-0.72, P=0.019, n=10; r=-0.65, P=0.042, n=10). In pregnancy, plasma ACE levels were related to protein/creatinine (r=0.29, P=0.036, n=54). Urinary protein/creatinine was not related to iRAS activity (males and non-pregnant females) but in pregnancy was related to prorenin and active renin/creatinine (r=0.45, P=0.02, n=26 r=0.47, P<0.001, n=50). Urinary albumin/creatinine was related to uAGT and active renin/creatinine in pregnancy (r=0.39, P=0.005, n=51; r=0.37, P=0.008, n=51). uACE/creatinine and uAGT/creatinine were related (r=0.52, P<0.001, n=51).
  
  
  CONCLUSIONS
  Excretion of components of the iRAS is independent of plasma levels. Not only is uAGT/creatinine related to albumin/creatinine but there are similar relationships with other iRAS components. Measurement of the iRAS in human pregnancy may detect early stage renal disease, endemic in Indigenous Australians.
authors.forename: Eugenie
authors.initials: E
authors.lastname: Lumbers
authors.forename: Kirsty
authors.initials: K
authors.lastname: Pringle
authors.forename: Shane
authors.initials: S
authors.lastname: Sykes
authors.forename: Loretta
authors.initials: L
authors.lastname: Weatherall
authors.forename: Don
authors.initials: D
authors.lastname: Clausen
authors.forename: Kym
authors.initials: K
authors.lastname: Rae
authors.forename: Roger
authors.initials: R
authors.lastname: Smith