title: CD10-positive mantle cell lymphoma: biologically distinct entity or an aberrant immunophenotype? Insight, through gene expression profile in a unique case series.
abstract: BACKGROUND
  Mantle cell lymphoma (MCL) is an aggressive disease with genetic heterogeneity and discrete clinical subtypes. MCL is rarely CD10 positive. These cases raise the question whether a subset of MCL may be germinal centre (GC) derived, and have distinct clinicopathological characteristics.
  
  
  OBJECTIVE
  A series of nine CD10-positive MCL cases is described herein. The clinicopathological and immunophenotypic features, immunoglobulin somatic hypermutation (SHM) status and gene expression profile (GEP) data are detailed. These features were compared with two independent sets (n=20, each) of CD10-negative MCL cases (controls), which were randomly selected from our institutional registry.
  
  
  RESULTS
  GEP showed distinct expression of a GC signature in CD10-positive MCL cases with minimal impact on downstream signalling pathways. There were no significant differences in the clinicopathological features or clinical outcome between our CD10-positive and CD10-negative MCL cases. The frequency of SHM was comparable with established data.
  
  
  CONCLUSIONS
  This study provides convincing evidence that CD10 expression is related to a distinct GC signature in MCL cases, but without clinical or biological implications.
authors.affiliation: Division of Hematology and Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Calgary/Calgary Laboratory Services (CLS), Calgary, Alberta, Canada.
authors.forename: Ariz
authors.initials: A
authors.lastname: Akhter
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Etienne
authors.initials: E
authors.lastname: Mahe
authors.affiliation: Division of Hematology and Hematological Malignancies, Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Lesley
authors.initials: L
authors.lastname: Street
authors.affiliation: Division of Hematology and Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Calgary/Calgary Laboratory Services (CLS), Calgary, Alberta, Canada.
authors.forename: Payam
authors.initials: P
authors.lastname: Pournazari
authors.affiliation: Division of Anatomical Pathology, Department of Pathology and Laboratory Medicine, University of Calgary/Calgary Laboratory Services (CLS), Calgary, Alberta, Canada.
authors.forename: Marco
authors.initials: M
authors.lastname: Perizzolo
authors.affiliation: Division of Hematology and Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Calgary/Calgary Laboratory Services (CLS), Calgary, Alberta, Canada.
authors.forename: Meer-Taher
authors.initials: MT
authors.lastname: Shabani-Rad
authors.affiliation: Division of Hematology and Hematological Malignancies, Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Stewart
authors.affiliation: Division of Hematology and Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Calgary/Calgary Laboratory Services (CLS), Calgary, Alberta, Canada.
authors.forename: Adnan
authors.initials: A
authors.lastname: Mansoor
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics*; D016022:Case-Control Studies; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D020522:Lymphoma, Mantle-Cell / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D015260:Neprilysin / Q000032:analysis*; D010641:Phenotype; D011237:Predictive Value of Tests; D012042:Registries
chemical_list: D014408:Biomarkers, Tumor; D015260:Neprilysin
keywords: IMMUNOCYTOCHEMISTRY; LYMPHOMA; MOLECULAR PATHOLOGY; TUMOUR BIOLOGY