title: PP038. Renal ETK/BMX activation decreased in preeclampsia.
abstract: BACKGROUND
  Vascular endothelial growth factors (VEGF's) are essential to angiogenesis and play a central role in the pathophysiology of preeclampsia. Specifically, antagonists of VEGFR2 cause a preeclampsia-like syndrome, in humans and rats[1]. ETK/BMX is a receptor tyrosine kinase (RTK) which induces VEGF expression and forms a complex with VEGFR2, whereby VEGF and TNF can induce a reciprocal activation of both kinases.
  
  
  OBJECTIVE
  To determine the levels of phosphorylation, and thus activation, of VEGFR2 and ETK/BMX in renal tissue from women with preeclampsia and with healthy pregnancies.
  
  
  METHODS
  Renal tissue was obtained with consent from six preeclamptic and six healthy pregnant women included in a previous renal needle biopsy study[2] and a RayBio® Phosphorylation Antibody Array was used according to instructions.
  
  
  RESULTS
  Phosphorylated ETK/BMX was significantly reduced in the preeclamptic women compared to in the healthy pregnant women. There was no difference in phosphorylated VEGFR2 between groups.
  
  
  CONCLUSIONS
  These data suggest that ETK/BMX could be an important mediator of VEGF function in healthy pregnancy, in the kidneys more so than VEGFR2, and that absence of the positive feedforward signalling that ETK/BMX and VEGF together accomplish, and/or a TNF induced activation of this, may play a role in the pathophysiology of preeclampsia.
authors.forename: Helena
authors.initials: H
authors.lastname: Strevens
authors.forename: Dag
authors.initials: D
authors.lastname: Wide-Swensson
authors.forename: Ola
authors.initials: O
authors.lastname: Hansson
authors.forename: Olle
authors.initials: O
authors.lastname: Melander