title: Associations of miRNA polymorphisms and expression levels with breast cancer risk in the Chinese population.
abstract: Single-nucleotide polymorphisms in microRNAs (miRNAs) may dramatically affect gene expression and subsequently alter individual susceptibility to cancer, and thus has become a research hotspot for many cancer types, including breast cancer. We recruited 321 breast cancer patients and 290 controls in our study. Four established miRNA single-nucleotide polymorphisms (mir-499 rs3746444 A>G; miR-27a rs895819 A>G; miR-196a2 rs11614913 T>C; miR-146a rs2910164 G/C) were detected using Taqman assays. Mature miRNA expression, allele distribution, and the association with clinical features were further analyzed. Our results showed that the miR146a rs2910164 G/C polymorphism was associated with an elevated risk of breast cancer (odds ratio = 1.85, 95% confidence interval = 1.03-3.32; P < 0.05). Compared with the ancestral T allele in miR-196a2 rs11614913, the variant C allele was consistently associated with an increased risk of breast cancer (odds ratio = 2.20, 95% confidence interval = 1.19-4.09, P < 0.01) and clinical pathological type (P < 0.01). miR-27a rs895819 A>G and miR-499 rs3746444 A>G were not associated with breast cancer risk. Analysis of mature miRNA expression confirmed that the variant C allele in miR146a rs2910164 and miR-196a2 rs11614913 dramatically inhibited production of their mature products. Our results suggested that miR-146a rs2910164 G>C and miR-196a2 rs11614913 T>C may be biomarkers for predicting breast cancer risk in the Chinese population.
authors.affiliation: Department of Head-Neck and Breast Surgery, Xinxiang Central Hospital, Xinxiang, China 413960747@qq.com.
authors.forename: P
authors.initials: P
authors.lastname: Qi
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: B
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Yao
authors.affiliation: Department of Head-Neck and Breast Surgery, Xinxiang Central Hospital, Xinxiang, China.
authors.forename: S
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Head-Neck and Breast Surgery, Xinxiang Central Hospital, Xinxiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Head-Neck and Breast Surgery, Xinxiang Central Hospital, Xinxiang, China.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Xie
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; C531388:MIRN27 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs