title: Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens.
abstract: The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.
authors.affiliation: Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain.
authors.forename: María Concepción
authors.initials: MC
authors.lastname: Mediavilla-Gradolph
authors.affiliation: Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: De Toro-Peinado
authors.affiliation: Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain.
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Bermúdez-Ruiz
authors.affiliation: Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain.
authors.forename: María de los Ángeles
authors.initials: Mde L
authors.lastname: García-Martínez
authors.affiliation: Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Ortega-Torres
authors.affiliation: Microbiology Unit, Hospital Costa del Sol, 29603 Marbella, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Montiel Quezel-Guerraz
authors.affiliation: Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, 29010 Malaga, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Palop-Borrás
mesh_terms: D005838:Genotype; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization