title: Mutagenesis of an Asn156 Residue in a Surface Region of S-Selective Hydroxynitrile Lyase from Baliospermum montanum Enhances Catalytic Efficiency and Enantioselectivity.
abstract: The S-selective hydroxynitrile lyase from Baliospermum montanum (BmHNL) has broad substrate specificity toward aromatic substrates as well as high temperature stability, although with low enantioselectivity and specific activity. To expand the industrial application of this enzyme, we improved its enantioselectivity and specific activity toward (S)-mandelonitrile by mutagenesis. The specific activity of the BmHNL H103C/N156G mutant for (S)-mandelonitrile production was raised to 154 U mg-1(WT BmHNL: 52 U mg-1). The enantiomeric excess was increased to 93 % (WT BmHNL: 55 %). The kinetic analysis revealed Kmfor (R)-mandelonitrile and kcatfor (S)-mandelonitrile increased by the mutation at Asn156, thus contributing to the increase in enantiomeric excess. This is the first report on an improvement in catalytic efficiency and enantiomeric excess of BmHNL for (S)-mandelonitrile synthesis by random and site-directed mutagenesis.
authors.affiliation: Biotechnology Research Center, Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398 (Japan).|Asano Active Enzyme Molucule Project, ERATO, JST, 5180 Kurokawa, Imizu, Toyama 939-0398 (Japan).
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kawahara
authors.affiliation: Biotechnology Research Center, Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398 (Japan).|Asano Active Enzyme Molucule Project, ERATO, JST, 5180 Kurokawa, Imizu, Toyama 939-0398 (Japan).
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
keywords: cyanohydrins; enzyme catalysis; hydroxynitrile lyase; mandelonitrile; mutagenesis