title: Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass.
abstract: BACKGROUND
  Cardiac surgery with cardiopulmonary bypass (CPB) may cause inflammatory responses, which can deteriorate the outcomes. Inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6,-8 and -10, can act as both the effector and the predictor for post-operative inflammatory responses. Plasma mitochondrial DNA (mtDNA) was found as a pro-inflammatory agent recently, which was released when cells were insulted.
  
  
  METHODS
  In the present study, we included 38 patients undergoing coronary artery bypass graft (CABG) to analyze their perioperative plasma mtDNA and levels of inflammatory cytokines. Blood samples were collected before aortic cross-clamping (T1), at the end of CPB (T2), 6 h post-CPB (T3), 12 h post-CPB (T4), and 24 h post-CPB (T5). Rt-PCR and specific ELISA kits were used to quantify the plasma mtDNA and inflammatory cytokines, respectively. Bivariate correlations analysis was used to check the correlations between plasma mtDNA and inflammatory cytokines respectively.
  
  
  RESULTS
  Results shown that plasma mtDNA elevated significantly at T2 and peaked at T4. Furthermore, plasma TNF-α, IL-6 and IL-8 levels significantly increased at T2 and peaked at T3 while IL-10 elevated and peaked at T2. Bivariate correlations analysis showed that the peak plasma mtDNA were positively correlated with the peak TNF-α (r = 0.677, P < 0.001), the peak IL-6 (r = 0.706, P < 0.001), the peak IL-8 (r = 0.584, P < 0.001) and the peak IL-10 (r = 0.565, P < 0.001).
  
  
  CONCLUSIONS
  We found that plasma mtDNA might play a key role in CPB-induced post-operative inflammatory responses.
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. joey.qin@outlook.com.
authors.forename: Chaoyi
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. rachael.liu@qq.com.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. 229554253@qq.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Cardiology, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. liyajiao800@163.com.
authors.forename: Yajiao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. qianhong2222@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. hxxwsyk@163.com.
authors.forename: Yingkang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. meng_wei_1111@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
mesh_terms: D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass / Q000379:methods*; D003324:Coronary Artery Disease / Q000097:blood* / Q000235:genetics / Q000601:surgery; D016207:Cytokines / Q000097:blood*; D004272:DNA, Mitochondrial / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D059035:Perioperative Period
chemical_list: D016207:Cytokines; D004272:DNA, Mitochondrial