title: Improving plant transformation using Agrobacterium tumefaciens.
abstract: Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in Eclipta alba explants.
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: L V
authors.initials: LV
authors.lastname: Ribeiro Neto
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Oliveira
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: M V
authors.initials: MV
authors.lastname: Lourenço
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: B W
authors.initials: BW
authors.lastname: Bertoni
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: S C
authors.initials: SC
authors.lastname: França
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: T M
authors.initials: TM
authors.lastname: Rosa-Santos
authors.affiliation: Laboratório de Genômica Funcional, Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil szingaretti@unaerp.br.
authors.forename: S M
authors.initials: SM
authors.lastname: Zingaretti
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D036601:Eclipta / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology / Q000528:radiation effects; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D007612:Kanamycin / Q000494:pharmacology; D007613:Kanamycin Resistance; D018547:Plant Stems / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology / Q000528:radiation effects; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D000069453:Ultrasonic Waves
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007612:Kanamycin