title: Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits.
abstract: BACKGROUND
  Platelet-rich plasma (PRP) contains numerous growth factors to promote wound healing and angiogenesis. The aim of this study was to gain further information about the benefits of platelet-rich-plasma for healing cutaneous acute to chronic wounds.
  
  
  METHODS
  A total of 30 New Zealand albino rabbits (n = 15/group) were randomly assigned to two experimental groups: control group, and PRP group. Bilateral resection defects measuring 3 cm were surgically created on the dorsolateral of the cutaneous in animals and the defects were randomly divided into two mentioned groups. Wound area, neovascularization, size and epithelialization were compared on days 7, 14 and 21 post-wounding. Histopathological analyses were conducted on 15 specimens from each group after sacrifice by the cellular aspects of the regeneration of the tissue.
  
  
  RESULTS
  Our results were indicated that the wound area of PRP was smaller than that in the non-treated group on days 7, 14 and 21. Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group. A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21. This results indicated that PRP treated group' enhanced angiogenesis at the wound beds as compared to no treatment group.
  
  
  CONCLUSIONS
  These results could be useful for researchers in the growing fields of tissue repair and experimental wound healing. Further studies will be essential to determine the role of PRP in clinical practice.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. omidostovar@yahoo.com.
authors.forename: Omid
authors.initials: O
authors.lastname: Ostvar
authors.affiliation: Brain and Spinal Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran. saharshadvar@gmail.com.
authors.forename: Sahar
authors.initials: S
authors.lastname: Shadvar
authors.affiliation: Baqiyatallah University of Medical Sciences, Tehran, Iran. emadyahaghi@yahoo.com.
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.affiliation: Department of Physical Medicine and Rehabilitation, Clinical Biomechanical and Rehabilitation Engineering research center, AJA University of Medical Sciences, Tehran, Iran. kamranazma@yahoo.com.
authors.forename: Kamran
authors.initials: K
authors.lastname: Azma
authors.affiliation: Department of Legal Medicine, AJA University of Medical Sciences, Tehran, Iran. amirfarshidfayyaz@yahoo.com.
authors.forename: Amir Farshid
authors.initials: AF
authors.lastname: Fayyaz
authors.affiliation: Department of Emergency Medicine, Alborz University of Medical Science, Karaj, Iran. ahmadik@mums.ac.ir.
authors.forename: Koorosh
authors.initials: K
authors.lastname: Ahmadi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453, Tehran, Iran. iradjnowrouzian@yahoo.com.
authors.forename: Iradj
authors.initials: I
authors.lastname: Nowrouzian
mesh_terms: D042501:Angiogenic Proteins / Q000097:blood*; D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D008297:Male; D018919:Neovascularization, Physiologic; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011817:Rabbits; D062326:Re-Epithelialization; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000097:blood / Q000473:pathology / Q000628:therapy*
chemical_list: D042501:Angiogenic Proteins; D036341:Intercellular Signaling Peptides and Proteins