title: MicroRNA-137 Contributes to Dampened Tumorigenesis in Human Gastric Cancer by Targeting AKT2.
abstract: MiRNAs play important roles in tumorigenesis. This study focused on exploring the effects and regulation mechanism of miRNA-137 on the biological behaviors of gastric cancer. Total RNA was extracted from tissues of 100 patients with gastric cancer and from four gastric cancer cell lines. Expression of miR-137 was detected by real-time PCR from 100 patients. The effects of miR-137 overexpression on gastric cancer cells' proliferation, apoptosis, migration and invasion ability were investigated in vitro and in vivo. The target gene of miR-137 was predicted by Targetscan on line software, screened by dual luciferase reporter gene assay and demonstrated by western blot. As a result, the expression of miR-137 was significant reduced in gastric cancer cell line HGC-27, HGC-803, SGC-7901 and MKN-45 as well as in gastric cancer tissues compared with GES-1 cell or matched adjacent non-neoplastic tissues (p<0.001). The re-introduction of miR-137 into gastric cancer cells was able to inhibit cell proliferation, migration and invasion. The in vivo experiments demonstrated that the miR-137 overexpression can reduce the gastric cancer cell proliferation and metastasis. Bioinformatic and western blot analysis indicated that the miR-137 acted as tumor suppressor roles on gastric cancer cells through targeting AKT2 and further affecting the Bad and GSK-3β. In conclusion, the miR-137 which is frequently down-regulated in gastric cancer is potentially involved in gastric cancer tumorigenesis and metastasis by regulating AKT2 related signal pathways.
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Shutang
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Xuejv
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, First affiliated Hospital of Henan University, Kaifeng, Henan, China.
authors.forename: Dazheng
authors.initials: D
authors.lastname: Han
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D015536:Down-Regulation; D064593:Heterografts; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: C545567:MIRN137 microRNA, human; D035683:MicroRNAs; C494921:AKT2 protein, human; D051057:Proto-Oncogene Proteins c-akt