title: Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.
abstract: Cotton (Gossypium spp.) is an important economic crop and there is obvious heterosis in cotton, fertility has played an important role in this heterosis. However, the genes that exhibit critical roles in anther development and fertility are not well understood. Here, we report an acyl-CoA N-acyltransferase (EC2.3; GhACNAT) that plays a key role in anther development and fertility. Suppression of GhACNAT by virus-induced gene silencing in transgenic cotton (G. hirsutum L. cv. C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility. We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis. The genes differentially expressed in GhACNAT-silenced plants and C312 were mainly involved in catalytic activity and transcription regulator activity in lipid metabolism. In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312. Treatments with exogenous methyl jasmonate rescued anther dehiscence and pollen release in GhACNAT-silenced plants and caused self-fertility. The GhACNAT gene may play an important role in controlling cotton fertility by regulating the pathways of lipid synthesis and JA biogenesis.
authors.affiliation: 1] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China [2] College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Fu
authors.affiliation: 1] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China [2] College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shen
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Cotton Biology, the Institute of Cotton Research of CAAS, Anyang, 455000, Henan, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ke
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Binglun
authors.initials: B
authors.lastname: Luo
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Xu
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Cheng
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection of CAAS, Beijing, 100193, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi University, Shihezi, 832000, Xinjiang Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Cotton Biology, the Institute of Cotton Research of CAAS, Anyang, 455000, Henan, China.
authors.forename: Chaozhu
authors.initials: C
authors.lastname: Xing
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D015256:Coenzyme A-Transferases / Q000737:chemistry / Q000502:physiology*; D017124:Conserved Sequence; D003517:Cyclopentanes / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis; D035264:Flowers / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000378:metabolism*; D051479:Plant Infertility; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D030821:Plants, Genetically Modified
chemical_list: D003517:Cyclopentanes; D005227:Fatty Acids; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D015256:Coenzyme A-Transferases