title: Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina.
abstract: Ninety-three rectal swab samples were taken, from dogs suspected of canine parvovirus (CPV) infection and analyzed by PCR. A fragment of the VP2 gene, was amplified in 41 (44%) of them, resulting CPV positive samples. Sequencing analysis of these PCR products showed that 37 samples (90.2%) belonged to the CPV2c type, whereas four samples (9.8%) were identified as CPV2a, which has not been found since 2008. It was also found that 24 out of 37 CPV2c samples (65%), carried the mutation Thr440Ala, whereas this mutation was absent in the four CPV2a strains reported herein. Using phylogenetic analysis of the full length VP2 gene, which was amplified by PCR in six local samples, it was seen that CPV2a Argentine strains reported in this study, were genetically closer to a previous local CPV2a isolate (year 2003) and to a South African CPV2a strain, than to any of the recently reported Uruguayan CPV2a strains. The results obtained in this work, together with those reported previously in Uruguay strongly suggest that, in spite of the geographical proximity, wild type CPV strains undergo different evolutive pathways in each country, resulting in the prevalence of different strains in related dog populations. Further extensive epidemiological studies are needed in order to improve the understanding of CPV evolution.
authors.affiliation: Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: marinagallocalderon@yahoo.com.ar.
authors.forename: Marina Gallo
authors.initials: MG
authors.lastname: Calderón
authors.affiliation: Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Carina
authors.initials: C
authors.lastname: Romanutti
authors.affiliation: Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Wilda
authors.affiliation: Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: D' Antuono
authors.affiliation: Fundación de Estudios en Virología Animal (FEVAN), Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Leticia
authors.initials: L
authors.lastname: Keller
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (UBA-CONICET), Facultad de Medicina, Paraguay 2155, p12, C1121ABG Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Mónica N
authors.initials: MN
authors.lastname: Giacomodonato
authors.affiliation: Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Nora
authors.initials: N
authors.lastname: Mattion
authors.affiliation: Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: José
authors.initials: J
authors.lastname: La Torre
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D016000:Cluster Analysis; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology; D004285:Dogs; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000302:isolation & purification*; D058974:Phylogeography; D016133:Polymerase Chain Reaction; D012007:Rectum / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
keywords: Canine parvovirus; Sequence analysis; VP2 gene; Virus evolution