title: Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture: Part 1 Design and feasibility.
abstract: Design, fabrication, integration, and feasibility test results of a novel microfluidic cell capture device is presented, exploiting the advantages of proton beam writing to make lithographic irradiations under multiple target tilting angles and UV lithography to easily reproduce large area structures. A cell capture device is demonstrated with a unique doubly tilted micropillar array design for cell manipulation in microfluidic applications. Tilting the pillars increased their functional surface, therefore, enhanced fluidic interaction when special bioaffinity coating was used, and improved fluid dynamic behavior regarding cell culture injection. The proposed microstructures were capable to support adequate distribution of body fluids, such as blood, spinal fluid, etc., between the inlet and outlet of the microfluidic sample reservoirs, offering advanced cell capture capability on the functionalized surfaces. The hydrodynamic characteristics of the microfluidic systems were tested with yeast cells (similar size as red blood cells) for efficient capture.
authors.affiliation: MTA Atomki, Debrecen, Hungary.
authors.forename: Istvan
authors.initials: I
authors.lastname: Rajta
authors.affiliation: MTA Atomki, Debrecen, Hungary.
authors.forename: Robert
authors.initials: R
authors.lastname: Huszánk
authors.affiliation: MTA Atomki, Debrecen, Hungary.
authors.forename: Atilla T T
authors.initials: AT
authors.lastname: Szabó
authors.affiliation: MTA Atomki, Debrecen, Hungary.
authors.forename: Gyula U L
authors.initials: GU
authors.lastname: Nagy
authors.affiliation: MTA Atomki, Debrecen, Hungary.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Szilasi
authors.affiliation: Hungarian Academy of Sciences, Centre for Energy Research, Institute of Technical Physics and Materials Science, Budapest, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Fürjes
authors.affiliation: Hungarian Academy of Sciences, Centre for Energy Research, Institute of Technical Physics and Materials Science, Budapest, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Holczer
authors.affiliation: Hungarian Academy of Sciences, Centre for Energy Research, Institute of Technical Physics and Materials Science, Budapest, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Fekete
authors.affiliation: MTA-PE Translational Glycomics Group, MUKKI, University of Pannonia, Veszprém, Hungary.
authors.forename: Gabor
authors.initials: G
authors.lastname: Járvás
authors.affiliation: MTA-PE Translational Glycomics Group, MUKKI, University of Pannonia, Veszprém, Hungary.|Horvath Csaba Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, Hungary.
authors.forename: Marton
authors.initials: M
authors.lastname: Szigeti
authors.affiliation: MTA-PE Translational Glycomics Group, MUKKI, University of Pannonia, Veszprém, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Hajba
authors.affiliation: MTA-PE Translational Glycomics Group, MUKKI, University of Pannonia, Veszprém, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Bodnár
authors.affiliation: MTA-PE Translational Glycomics Group, MUKKI, University of Pannonia, Veszprém, Hungary.|Horvath Csaba Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, Hungary.
authors.forename: Andras
authors.initials: A
authors.lastname: Guttman
mesh_terms: D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005240:Feasibility Studies; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008954:Models, Biological; D015003:Yeasts / Q000166:cytology
keywords: Cell capture; Proton beam writing; Soft lithography