title: Integrated miRNA-mRNA analysis of Epstein-Barr virus-positive nasopharyngeal carcinoma.
abstract: This study aims to identify the crucial miRNAs in Epstein-Barr virus-positive nasopharyngeal carcinoma (NPC) and their target genes. Gene expression profile data (GSE12452) that included 31 NPC and 10 normal nasopharyngeal tissue specimens were downloaded. Differentially expressed genes (DEGs) were identified using significance analysis of microarrays. The underlying function of DEGs was predicted via Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. The miRNA sequencing dataset GSE14738 was also downloaded, and expression levels of miRNA were calculated by the number of reads mapped to each miRNA. The selected miRNAs were integrated into the miRecords database to obtain their target genes. Target genes associated with DEGs were used to construct the interaction network via Cytoscape. A total of 1437 DEGs between NPC and control were identified, most of which were enriched in cell cycle and extracellular matrix-receptor interaction signaling pathways. Furthermore, 112 miRNAs were considered upregulated in NPC samples. A total of 2228 relationships between 39 miRNAs and 1247 target genes were obtained, of which 182 relationships between 32 miRNAs and 97 target genes were chosen to construct an interaction network. The interactions between DEGs and the let-7 or miR-29 families appeared strongest in this network, where CDC25A, COL3A1, and COL1A1 were regulated by several let-7 family members, while COL4A1 and COL5A2 were regulated by several miR-29 family members. The let-7 and miR-29 families may be related to the development of NPC by regulating the genes involved in cell cycle and ECM-receptor interaction.
authors.affiliation: Department of Otolaryngology & Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University.
authors.forename: L H
authors.initials: LH
authors.lastname: Zhu
authors.affiliation: Department of Otolaryngology & Head and Neck Surgery, Beijing Jishuitan Hospital, XiCheng District, Beijing, China.
authors.forename: X T
authors.initials: XT
authors.lastname: Miao
authors.affiliation: Chaoyang District, Beijing, China wangningyyoo@163.com.
authors.forename: N Y
authors.initials: NY
authors.lastname: Wang
mesh_terms: D002277:Carcinoma; D019295:Computational Biology / Q000379:methods; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger