title: Investigation of irradiated rats DNA in the presence of Cu(II) chelates of amino acids Schiff bases.
abstract: The new synthesized Cu(II) chelates of amino acids Schiff bases were studied as a potential radioprotectors. Male albino rats of Wistar strain were exposed to X-ray whole-body irradiation at 4.8 Gy. This dose caused 30% mortality of the animals (LD30). The survival of animals exposed to radiation after preliminary administration of 10 mg/kg Cu(II)(Nicotinyl-L-Tyrosinate)2 or Cu(II)(Nicotinyl-L-Tryptophanate)2 prior to irradiation was registered about 80 and 100% correspondingly. Using spectrophotometric melting and agarose gel electrophoresis methods, the differences between the DNA isolated from irradiated rats and rats pretreated with Cu(II) chelates were studied. The fragments of DNA with different breaks were revealed in DNA samples isolated from irradiated animals. While, the repair of the DNA structure was observed for animals pretreated with the Cu(II) chelates. The results suggested that pretreatment of the irradiated rats with Cu(II)(Nicotinyl-L-Tyrosinate)2 and Cu(II)(Nicotinyl-L-Tryptophanate)2 compounds improves the liver DNA characteristics.
authors.affiliation: a Department of Physics , Yerevan State University , Al. Manoogian 1, Yerevan 0025 , Armenia.
authors.forename: N H
authors.initials: NH
authors.lastname: Karapetyan
authors.affiliation: a Department of Physics , Yerevan State University , Al. Manoogian 1, Yerevan 0025 , Armenia.
authors.forename: A L
authors.initials: AL
authors.lastname: Torosyan
authors.affiliation: b Centre of Radiation Medicine and Burns , P.O. Box 25, Davidashen, Yerevan 0048 , Armenia.
authors.forename: M
authors.initials: M
authors.lastname: Malakyan
authors.affiliation: b Centre of Radiation Medicine and Burns , P.O. Box 25, Davidashen, Yerevan 0048 , Armenia.
authors.forename: S A
authors.initials: SA
authors.lastname: Bajinyan
authors.affiliation: a Department of Physics , Yerevan State University , Al. Manoogian 1, Yerevan 0025 , Armenia.
authors.forename: S G
authors.initials: SG
authors.lastname: Haroutiunian
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002614:Chelating Agents / Q000008:administration & dosage / Q000737:chemistry*; D003300:Copper / Q000008:administration & dosage / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D011827:Radiation; D011837:Radiation-Protective Agents / Q000737:chemistry*; D051381:Rats; D012545:Schiff Bases / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002614:Chelating Agents; D011837:Radiation-Protective Agents; D012545:Schiff Bases; D003300:Copper; D004247:DNA
keywords: DNA; X-ray irradiation; electrophoresis; melting