title: Expanding the Entamoeba Universe: New Hosts Yield Novel Ribosomal Lineages.
abstract: Removing the requirement for cell culture has led to a substantial increase in the number of lineages of Entamoeba recognized as distinct. Surveying the range of potential host species for this parasite genus has barely been started and it is clear that additional sampling of the same host in different locations often identifies additional diversity. In this study, using small subunit ribosomal RNA gene sequencing, we identify four new lineages of Entamoeba, including the first report of Entamoeba from an elephant, and extend the host range of some previously described lineages. In addition, examination of microbiome data from a number of host animals suggests that substantial Entamoeba diversity remains to be uncovered.
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, United Kingdom.
authors.forename: Alison S
authors.initials: AS
authors.lastname: Jacob
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, United Kingdom.
authors.forename: Eloise J
authors.initials: EJ
authors.lastname: Busby
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, United Kingdom.
authors.forename: Abigail D
authors.initials: AD
authors.lastname: Levy
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, United Kingdom.
authors.forename: Natasha
authors.initials: N
authors.lastname: Komm
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, United Kingdom.
authors.forename: C Graham
authors.initials: CG
authors.lastname: Clark
mesh_terms: D000818:Animals; D001188:Artiodactyla / Q000469:parasitology; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal; D004606:Elephants / Q000469:parasitology; D004747:Entamoeba / Q000145:classification* / Q000502:physiology*; D004749:Entamoebiasis / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D020459:Genes, rRNA; D014644:Genetic Variation; D006734:Horse Diseases / Q000469:parasitology; D006736:Horses; D058507:Host Specificity; D064307:Microbiota; D010802:Phylogeny; D054679:Ribosome Subunits, Small / Q000235:genetics; D012376:Rodent Diseases / Q000469:parasitology; D012377:Rodentia; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Diversity; genetic diversity; next generation sequencing; phylogeny; ribosomal RNA; sequencing