title: Sox2-CreER mice are useful for fate mapping of mature, but not neonatal, cochlear supporting cells in hair cell regeneration studies.
abstract: Studies of hair cell regeneration in the postnatal cochlea rely on fate mapping of supporting cells. Here we characterized a Sox2-CreER knock-in mouse line with two independent reporter mouse strains at neonatal and mature ages. Regardless of induction age, reporter expression was robust, with CreER activity being readily detectable in >85% of supporting cells within the organ of Corti. When induced at postnatal day (P) 28, Sox2-CreER activity was exclusive to supporting cells demonstrating its utility for fate mapping studies beyond this age. However, when induced at P1, Sox2-CreER activity was also detected in >50% of cochlear hair cells, suggesting that Sox2-CreER may not be useful to fate map a supporting cell origin of regenerated hair cells if induced at neonatal ages. Given that this model is currently in use by several investigators for fate mapping purposes, and may be adopted by others in the future, our finding that current protocols are effective for restricting CreER activity to supporting cells at mature but not neonatal ages is both significant and timely.
authors.affiliation: Dept. of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Walters
authors.affiliation: Dept. of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamashita
authors.affiliation: Dept. of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Zuo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division; D054338:Cell Transdifferentiation; D006198:Hair Cells, Auditory / Q000166:cytology / Q000378:metabolism*; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009925:Organ of Corti / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D012038:Regeneration; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D008164:Luminescent Proteins; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C045073:Cre recombinase; D019426:Integrases