title: Cyclometalated Iridium(III) Imidazole Phenanthroline Complexes as Luminescent and Electrochemiluminescent G-Quadruplex DNA Binders.
abstract: Six cyclometalated iridium(III) phenanthroimidazole complexes with different modifications to the imidazole phenanthroline ligand exhibit enhanced luminescence when bound to guanine (G-) quadruplex DNA sequences. The complexes bind with low micromolar affinity to human telomeric and c-myc sequences in a 1:1 complex:quadruplex stoichiometry. Due to the luminescence enhancement upon binding to G-quadruplex DNA, the complexes can be used as selective quadruplex indicators. In addition, the electrogenerated chemiluminescence of all complexes increases in the presence of specific G-quadruplex sequences, demonstrating potential for the development of an ECL-based G-quadruplex assay.
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Castor
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Metera
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Ushula M
authors.initials: UM
authors.lastname: Tefashe
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Serpell
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Janine
authors.initials: J
authors.lastname: Mauzeroll
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke West, Montreal, Quebec H3A 0B8, Canada.
authors.forename: Hanadi F
authors.initials: HF
authors.lastname: Sleiman
mesh_terms: D001665:Binding Sites; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry / Q000378:metabolism; D007093:Imidazoles / Q000737:chemistry* / Q000378:metabolism; D007495:Iridium / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D008958:Models, Molecular; D010618:Phenanthrolines / Q000737:chemistry* / Q000378:metabolism
chemical_list: D056831:Coordination Complexes; D007093:Imidazoles; D008024:Ligands; D049408:Luminescent Agents; D010618:Phenanthrolines; D007495:Iridium; D006147:Guanine; C029899:imidazole