title: Prevention of Serum Albumin Glycation/Fibrillation by β-Cyclodextrin Functionalized Magnetic Nanoparticles.
abstract: Nowadays, glycation induced protein aggregation and related opposing strategies have received much attention. We present the effect of functionalized magnetic core-shell nanoparticles of Fe3O4 (MNPs) with β-cyclodextrin (β-CD) on the aggregation/fibrillation of bovine serum albumin (BSA) under diabetic condition known as amyloidogenesis. To confirm the β-CD conjugation on MNP, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM) methods were applied. Moreover, spectrofluorimetry and spectropolarimetry were utilized to analyze the effect of β-CD/Fe3O4 MNPs on the aggregation and amyloidogenesis of BSA through glycation. The BSA amyloidogenesis was significantly inhibited by interfering β-CD-MNPs that may present the possible diagnostic and preventive applications against the degenerative effects of protein glycation/fibrillation under diabetes.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Ansari
authors.affiliation: School of Biology, College of Science, University of Tehran, Tehran, Iran. mhabibi@ut.ac.ir.
authors.forename: Mehran
authors.initials: M
authors.lastname: Habibi-Rezaei
authors.forename: Soheila
authors.initials: S
authors.lastname: Salahshour-Kordestani
authors.forename: Ali A Moosavi
authors.initials: AA
authors.lastname: Movahedi
authors.forename: Najmeh
authors.initials: N
authors.lastname: Poursasan
mesh_terms: D000818:Animals; D002417:Cattle; D006031:Glycosylation / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D055503:Protein Multimerization / Q000187:drug effects*; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D058185:Magnetite Nanoparticles; D047392:beta-Cyclodextrins; D012710:Serum Albumin, Bovine; C031215:betadex