title: Association between ApoA-II -265T/C polymorphism and oxidative stress in patients with type 2 diabetes mellitus.
abstract: BACKGROUND
  Apolipoprotein A-II (ApoA-II) constitutes approximately 20% of the total HDL protein content. The results of various studies on the relationship between cardiovascular diseases (CVD) and the plasma ApoA-II level are contradictory. The aim of this study was to determine the relationship between ApoA-II polymorphism and oxidative stress (OS) as a risk factor for CVD.
  
  
  METHODS
  The present comparative study was carried out on 180 obese and non-obese patients with type 2 diabetes, with equal numbers of CC, TC, and TT genotypes of ApoA-II -265T/C gene. The ApoA-II genotype was determined by the TaqMan assay method. The anthropometric measurements and serum levels of lipid profile, superoxide dismutase activity (SOD), total antioxidant capacity (TAC), and 8-isoprostaneF2α were measured.
  
  
  RESULTS
  After adjusting for confounding factors, in the total study population and in obese and non-obese groups, the subjects with CC genotype had a lower mean serum SOD activity (p=0.002, p=0.007 and p=0.005, respectively) and higher mean 8-isoprostaneF2α concentration (p<0.001, p=0.003 and p=0.004, respectively) than the T-allele carriers. In the TT/TC group, the mean 8-isoprostanF2α concentration was significantly higher in the obese subjects than the non-obese subjects (p=0.009). In the CC group, no significant differences were found in the OS factors between obese and non-obese groups.
  
  
  CONCLUSIONS
  The T allele in patients with type 2 diabetes is a protective factor against OS; obesity inhibits this protective effect. The results of this study represent the anti-atherogenic properties of ApoA-II. However, further studies are needed in this field.
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran; Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Koohdani
authors.affiliation: Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Haleh
authors.initials: H
authors.lastname: Sadrzadeh-Yeganeh
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Djalali
authors.affiliation: Department of Biostatistics and Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Eshraghian
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Keramat
authors.affiliation: Department of Biostatistics and Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Mansournia
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: elh.zamani@yahoo.com.
authors.forename: Elham
authors.initials: E
authors.lastname: Zamani
mesh_terms: D000367:Age Factors; D000886:Anthropometry; D016633:Apolipoprotein A-II / Q000235:genetics*; D015992:Body Mass Index; D002318:Cardiovascular Diseases / Q000453:epidemiology* / Q000503:physiopathology; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D008297:Male; D015999:Multivariate Analysis; D009765:Obesity / Q000453:epidemiology; D018384:Oxidative Stress / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012016:Reference Values; D018570:Risk Assessment; D012737:Sex Factors; D015996:Survival Rate
chemical_list: D016633:Apolipoprotein A-II
keywords: ApoA-II polymorphism; Cardiovascular disease; Diabetes; Obesity; Oxidative stress