title: IL-10 and IL-17F Promoter Single Nucleotide Polymorphism and Asthma: A Case-Control Study in South India.
abstract: BACKGROUND
  Several studies have assessed the association between IL-17F and IL-10 promoter polymorphisms and asthma, but the results were conflicting. Furthermore, few studies have evaluated the association of cytokine polymorphisms with asthma and its clinical phenotypes.
  
  
  OBJECTIVE
  This study was conducted to evaluate the association of IL-10 (interleukin 10) and IL-17F (interleukin 17F) promoter polymorphisms (rs1800871, rs1800896 and rs1889570) with asthma and its clinical phenotypes including severity, atopic status, spirometric parameters, and response to treatment in south Indian population. A sub-study was conducted to assess cytokine levels in subjects with different gene variants.
  
  
  METHODS
  IL-10 and IL-17F polymorphisms were genotyped in 419 asthmatic patients and 393 controls using Mass ARRAY.
  
  
  RESULTS
  Our results showed an association between IL-10 SNPs and mild asthma. No association was found with any of three SNPs in moderate to severe asthma. Comparison of genotype distribution of IL-17F rs1887570 AA variant among atopic and non-atopic patients showed significant difference (p = 0.024). Correlation analysis of IL-10 and IL-17F SNPs to clinical variables showed a positive correlation between IL-17F rs1887570 AA and number of allergen sensitized (rs = 0.142, p = 0.004). Significant improvement in lung function was observed after 2 months of ICS (Inhaled corticosteroids) and LABA (long acting β2 agonist) treatment in all subjects with no statistically significant difference among SNPs variants. Cytokines levels were similar in different SNP variants.
  
  
  CONCLUSIONS
  We observed an association between IL-10 rs1800871 and rs1800896 SNPs and mild asthma, as well as IL-17F rs1887570 AA variant and number of allergens sensitized.
authors.affiliation: Genetics and Genomics Lab, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
authors.forename: Sareh
authors.initials: S
authors.lastname: Raeiszadeh Jahromi
authors.affiliation: Department of Pulmonary Medicine, J.S.S. Medical College, Mysore, Karnataka, India.
authors.forename: P A
authors.initials: PA
authors.lastname: Mahesh
authors.affiliation: Department of Pulmonary Medicine, J.S.S. Medical College, Mysore, Karnataka, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Jayaraj
authors.affiliation: Allergy, Asthma, and Chest Center, Mysore, Karnataka, India.
authors.forename: Amrutha D
authors.initials: AD
authors.lastname: Holla
authors.affiliation: Genetics and Genomics Lab, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Vishweswaraiah
authors.affiliation: Genetics and Genomics Lab, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore, Karnataka, India. nbruom@gmail.com.
authors.forename: Nallur B
authors.initials: NB
authors.lastname: Ramachandra
mesh_terms: D000328:Adult; D000483:Alleles; D001249:Asthma / Q000188:drug therapy / Q000235:genetics* / Q000503:physiopathology*; D016022:Case-Control Studies; D005260:Female; D005541:Forced Expiratory Volume; D005838:Genotype; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics; D007194:India; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D020381:Interleukin-17 / Q000097:blood / Q000235:genetics*; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012720:Severity of Illness Index; D013147:Spirometry; D014797:Vital Capacity
chemical_list: D020381:Interleukin-17; D016753:Interleukin-10
keywords: Asthma; Atopic status; IL-10; IL-17F; Promoter; Single nucleotide polymorphism