title: Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs.
abstract: The bactericidal/permeability-increasing protein (BPI) gene has been identified as a candidate gene for disease-resistance breeding. We evaluated whether polymorphisms in exons 4 and 10 of the BPI gene are associated with immune indices [interleukin-2 (IL-2), IL-4, IL-6, interferon-b (IFN-b), IL-10, and IL-12]. In this study, we identified one mutation (C522T) in the BPI exon 4 site and two mutations (A1060G and T1151G) in the BPI exon 10 site. Correlation analysis revealed that in the Sutai pig population, the effect of genotypes at the BPI exon 4 site on the level of IL-6 was significant (P < 0.05), with an effective genotype of CD; moreover, the effect of genotypes at the BPI exon 10 site on the level of IL-12 was significant (P < 0.05), and the effective genotype was AB. The optimal combined genotype was CD-AB, which was more effective regarding the IL-6 and IL-12 levels compared to the other combined genotypes (P < 0.05). These results indicate that single nucleotide polymorphisms and the combined genotypes of BPI exons 4 and 10 affect immune indices in Sutai pigs. Therefore, these genotypes should be further examined as effective markers for disease-resistant breeding of pigs.
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors.forename: S P
authors.initials: SP
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Huo
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China wenbinbao74@yahoo.com.
authors.forename: W B
authors.initials: WB
authors.lastname: Bao
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D001798:Blood Proteins / Q000235:genetics*; D060467:Disease Resistance; D005091:Exons; D007376:Interleukin-2 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D000068618:Selective Breeding; D013552:Swine / Q000235:genetics / Q000276:immunology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D007376:Interleukin-2; D015850:Interleukin-6; C021623:bactericidal permeability increasing protein