title: Brain activity mapping at multiple scales with silicon microprobes containing 1,024 electrodes.
abstract: The coordinated activity of neural ensembles across multiple interconnected regions has been challenging to study in the mammalian brain with cellular resolution using conventional recording tools. For instance, neural systems regulating learned behaviors often encompass multiple distinct structures that span the brain. To address this challenge we developed a three-dimensional (3D) silicon microprobe capable of simultaneously measuring extracellular spike and local field potential activity from 1,024 electrodes. The microprobe geometry can be precisely configured during assembly to target virtually any combination of four spatially distinct neuroanatomical planes. Here we report on the operation of such a device built for high-throughput monitoring of neural signals in the orbitofrontal cortex and several nuclei in the basal ganglia. We perform analysis on systems-level dynamics and correlations during periods of conditioned behavioral responding and rest, demonstrating the technology's ability to reveal functional organization at multiple scales in parallel in the mouse brain.
authors.affiliation: Department of Neurobiology, University of California, Los Angeles, California;
authors.forename: Justin L
authors.initials: JL
authors.lastname: Shobe
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, California;
authors.forename: Leslie D
authors.initials: LD
authors.lastname: Claar
authors.affiliation: Neuroscience Interdepartmental Program, University of California, Los Angeles, California;
authors.forename: Sepideh
authors.initials: S
authors.lastname: Parhami
authors.affiliation: Neuroscience Interdepartmental Program, University of California, Los Angeles, California;
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Bakhurin
authors.affiliation: Department of Neurobiology, University of California, Los Angeles, California; Department of Bioengineering, University of California, Los Angeles, California; Neuroscience Interdepartmental Program, University of California, Los Angeles, California; Integrative Center for Learning and Memory, University of California, Los Angeles, California; and California NanoSystems Institute, University of California, Los Angeles, California smasmanidis@ucla.edu.
authors.forename: Sotiris C
authors.initials: SC
authors.lastname: Masmanidis
mesh_terms: D000200:Action Potentials; D000818:Animals; D001479:Basal Ganglia / Q000502:physiology*; D001931:Brain Mapping / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004569:Electroencephalography / Q000295:instrumentation* / Q000379:methods; D005625:Frontal Lobe / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012825:Silicon
chemical_list: D012825:Silicon
keywords: basal ganglia; high-density electrodes; multielectrodes; orbitofrontal cortex; silicon probes