title: Integration of DNA bio-gates and duplex-specific nuclease signal amplification: towards electrochemiluminescence detection of survivin mRNA.
abstract: Using the DNA bio-gate and duplex-specific nuclease assisted target recycling, a facile electrochemiluminescence assay was developed for the sensitive detection of survivin mRNA.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, Jiangsu, P. R. China. xujj@nju.edu.cn hychen@nju.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiang-Ling
authors.initials: XL
authors.lastname: Li
authors.forename: Jia-Dong
authors.initials: JD
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Hao
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry; D003851:Deoxyribonucleases / Q000737:chemistry; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D049449:Luminescence; D053758:Nanoparticles / Q000145:classification; D009942:Organometallic Compounds / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009942:Organometallic Compounds; D012333:RNA, Messenger; D000077022:Survivin; C415768:tris(2,2'-bipyridyl)ruthenium(II); D012822:Silicon Dioxide; D004247:DNA; D003851:Deoxyribonucleases