title: Identification and mapping stripe rust resistance gene YrLM168a using extreme individuals and recessive phenotype class in a complicate genetic background.
abstract: The identification and characterization of resistance genes effective against stripe rust of wheat is beneficial for modern wheat breeding programs. Molecular markers to such genes facilitate their deployment. The variety Milan has resistance that is effective against the predominant stripe rust races in the Sichuan region. Two resistant and two susceptible F8 lines from a cross between Milan and the susceptible variety Chuannong 16 were used to investigate inheritance of the Milan resistance. Three F2 populations were developed from crosses between the resistant lines and their susceptible sibling lines (LM168a × LM168c, LM168c × LM168a, LM168b × LM168d) and used for genetic analysis and molecular mapping of the genes for resistance. The stripe rust resistance in LM168a and LM168b was conferred by a single dominant gene, temporarily designated as YrLM168a. Forty-five extreme susceptible plants from the F2 families of LM168d × LM168b were genotyped with 836 simple sequence repeat (SSR) markers to map YrLM168a. YrLM168a was mapped in chromosome 6BL. The nearest flanking markers Xwmc756 and Xbarc146 were 4.6 and 4.6 cM away from the gene at both sides, respectively. The amplification results of twenty extreme resistant (IT 0) and susceptible (IT 4) F2 plants of LM168c × LM168a and LM168a × LM168c with marker Xwmc756 further validated the mapping results. The study suggested that extreme individuals and recessive phenotype class can be successfully used for mapping genes, which should be efficient and reliable. In addition, the flanking markers near YrLM168a should be helpful in marker-assisted breeding.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China. junyanfeng@live.cn.|Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610061, Sichuan, People's Republic of China. junyanfeng@live.cn.
authors.forename: Junyan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Guoyue
authors.initials: G
authors.lastname: Chen
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Yaxi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Qiantao
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Zhien
authors.initials: Z
authors.lastname: Pu
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Xiujin
authors.initials: X
authors.lastname: Lan
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Shoufen
authors.initials: S
authors.lastname: Dai
authors.affiliation: Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610061, Sichuan, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China. ylzheng@sicau.edu.cn.
authors.forename: Youliang
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D005658:Fungi / Q000472:pathogenicity*; D017343:Genes, Plant; D005808:Genes, Recessive; D008040:Genetic Linkage; D005819:Genetic Markers; D010641:Phenotype; D014908:Triticum / Q000382:microbiology*
chemical_list: D005819:Genetic Markers
keywords: Molecular mapping; Resistance gene; Simple sequence repeat (SSR); Wheat stripe rust