title: Report of new cases of Trypanosoma caninum in Brazil.
abstract: BACKGROUND
  Trypanosoma caninum is a protozoan species recently described in dogs, whose occurrence has been reported in areas of overlap with visceral leishmaniasis.
  
  
  METHODS
  Trypanosoma sp. were isolated from nine dogs and characterized by molecular methods.
  
  
  RESULTS
  PCR and sequencing confirmed the presence of T. caninum in all dogs, revealing two new areas of transmission: Barra Mansa and São João do Piauí.
  
  
  CONCLUSIONS
  The nine new cases described, when added to those already published, account for 62 cases of natural infection by T. caninum and show the geographical spread of this species to new areas, where other trypanosomatids circulate.
authors.affiliation: Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Tatiana da Silva Fonseca
authors.initials: Tda S
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Juliana Helena Silva
authors.initials: JH
authors.lastname: Barros
authors.affiliation: Laboratório de Doenças Parasitárias, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Taliha Dias
authors.initials: TD
authors.lastname: Perez
authors.affiliation: Laboratório de Pesquisa Clínica em Dermatozoonoses, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Fabiano Borges
authors.initials: FB
authors.lastname: Figueiredo
authors.affiliation: Laboratório de Pesquisa Clínica em Dermatozoonoses, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Artur Augusto Velho Mendes
authors.initials: AA
authors.lastname: Júnior
authors.affiliation: Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Maria de Fátima
authors.initials: Mde F
authors.lastname: Madeira
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D014345:Trypanosoma / Q000145:classification / Q000235:genetics*; D014352:Trypanosomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D012337:RNA, Ribosomal, 18S