title: The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans.
abstract: Aging is the major risk factor for many human diseases and degeneration. Thus, clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted. In traditional Chinese medicine (TCM), some were claimed to slow down aging. Qingyangshen (Cynanchum otophyllum schneid) is such a TCM. Here, we assayed the longevity effect of compound Otophylloside B (Ot B), a C-21 steroidal glycoside isolated from Qingyangshen, in Caenorhabditis elegans, which is a popular model for aging research. Our results showed that Ot B could modestly extend the lifespan of C. elegans, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16. Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor (daf-2). In addition, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.|University of Chinese Academy of Sciences, Beijing, 100049 China.|The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101 China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.|University of Chinese Academy of Sciences, Beijing, 100049 China.
authors.forename: Qin-Li
authors.initials: QL
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.
authors.forename: Quan-Zhang
authors.initials: QZ
authors.lastname: Mu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.|University of Chinese Academy of Sciences, Beijing, 100049 China.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.|University of Chinese Academy of Sciences, Beijing, 100049 China.
authors.forename: Zhong-Lin
authors.initials: ZL
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming, 650201 Yunnan China.
authors.forename: Huai-Rong
authors.initials: HR
authors.lastname: Luo
keywords: Aging; Caenorhabditis elegans; DAF-16/FOXO; IIS signaling pathway; Otophylloside B