title: Gypsophila elegans isoorientin attenuates CCl₄-induced hepatic fibrosis in rats via modulation of NF-κB and TGF-β1/Smad signaling pathways.
abstract: The hepatoprotective effect of Gypsophila elegans isoorientin (GEI) was evaluated using a hepatic fibrosis model induced by CCl4 in rats. The results revealed that GEI significantly prevented CCl4-induced liver injury and fibrosis, as evidenced by the attenuation of histopathological changes, the decrease in serum aminotransferase, and the inhibition of collagen accumulation. GEI strongly inhibited lipid peroxidation and recruited anti-oxidative defense system. Moreover, GEI alleviated pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6 via inhibiting nuclear factor-κB (NF-κB) activation. In addition, GEI down-regulated the phosphorylation of Smad2/3 and up-regulated the level of hepatic Smad7, thereby inhibiting TGFβ1/Smad signaling pathway. In conclusion, our findings indicate that GEI can inhibit CCl4-induced hepatic fibrosis, which may be ascribed to its radical scavenging action, antioxidant activity, and modulation of NF-κB and TGF-β1/Smad signaling pathways.
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Lv
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Shimei
authors.initials: S
authors.lastname: Tan
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Zhuo
authors.affiliation: The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China.
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Lu
authors.affiliation: The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China. Electronic address: hqf00@126.com.
authors.forename: Quanfang
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control*; D029748:Caryophyllaceae / Q000737:chemistry*; D016207:Cytokines / Q000097:blood; D016166:Free Radical Scavengers / Q000494:pharmacology; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D047311:Luteolin / Q000494:pharmacology*; D008297:Male; D020782:Matrix Metalloproteinases / Q000187:drug effects; D016328:NF-kappa B / Q000187:drug effects*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D051785:Smad Proteins / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000187:drug effects*
chemical_list: D016207:Cytokines; D016166:Free Radical Scavengers; D016328:NF-kappa B; D051785:Smad Proteins; D053773:Transforming Growth Factor beta1; C057912:homoorientin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D020782:Matrix Metalloproteinases; D047311:Luteolin
keywords: Gypsophila elegans; Hepatic fibrosis; Isoorientin; NF-κB; TGF-β1/Smad