title: Construction of extracellular microenvironment to improve surface endothelialization of NiTi alloy substrate.
abstract: To mimic extracellular microenvironment of endothelial cell, a bioactive multilayered structure of gelatin/chitosan pair, embedding with vascular endothelial growth factor (VEGF), was constructed onto NiTi alloy substrate surface via a layer-by-layer assembly technique. The successful fabrication of the multilayered structure was demonstrated by scanning electron microscopy, atomic force microscopy, contact angle measurement, attenuated total reflection-fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The growth behaviors of endothelial cells on various NiTi alloy substrates were investigated in vitro. Cytoskeleton observation, MTT assay, and wound healing assay proved that the VEGF-embedded multilayer structure positively stimulated adhesion, proliferation and motogenic responses of endothelial cells. More importantly, the present system promoted the nitric oxide production of endothelial cells. The approach affords an alternative to construct extracellular microenvironment for improving surface endothelialization of a cardiovascular implant.
authors.affiliation: Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China. Electronic address: liupeng79@cqu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Weihu
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address: kaiyong_cai@cqu.edu.cn.
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Cai
mesh_terms: D000497:Alloys / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009532:Nickel / Q000737:chemistry* / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D056951:Photoelectron Spectroscopy / Q000379:methods; D019736:Prostheses and Implants; D013499:Surface Properties / Q000187:drug effects*; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000497:Alloys; D042461:Vascular Endothelial Growth Factor A; C040654:titanium nickelide; D009569:Nitric Oxide; D009532:Nickel; D005780:Gelatin; D048271:Chitosan; D014025:Titanium
keywords: Endothelialization; Extracellular microenvironment; Layer-by-layer assembly; Surface modification; Vascular endothelial growth factor