title: One-pot synthesis of porphyrin functionalized γ-Fe2O3 nanocomposites as peroxidase mimics for H2O2 and glucose detection.
abstract: Meso-tetrakis(4-carboxyphenyl)-porphyrin-functionalized γ-Fe2O3 nanoparticles (H2TCPP-γ-Fe2O3) were successfully prepared by one-pot method under hydrothermal conditions and were found to possess intrinsic peroxidase-like activity. The H2TCPP-γ-Fe2O3 nanocomposites can catalytically oxidize peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction, which can be easily observed by the naked eye. Furthermore, kinetic studies indicate that the H2TCPP-γ-Fe2O3 nanocomposites have an even higher affinity to TMB than that of the natural enzyme, horseradish peroxidase (HRP). On the basis of the high activity, the reaction provides a simple, sensitive and selective method for colorimetric detection of H2O2 over a range of 10-100 μM with a minimum detection limit of 1.73 μM. Moreover, H2TCPP-γ-Fe2O3/glucose oxidase (GOx)/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 5-25 μM with a minimum detection limit of 2.54 μM. The results indicated that it is a simple, cheap, convenient, highly selective, sensitive and easy handling colorimetric assay. Results of a fluorescent probe suggest that the catalase-mimic activity of the H2TCPP-γ-Fe2O3 nanocomposites effectively catalyze the decomposition of H2O2 into H2O and O2.
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China. Electronic address: qyliu@sdust.edu.cn.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Leyou
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Jia
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Renren
authors.initials: R
authors.lastname: Zhu
mesh_terms: D001560:Benzidines / Q000737:chemistry; D002384:Catalysis; D060326:Chemistry Techniques, Synthetic; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D018360:Crystallography, X-Ray; D005290:Ferric Compounds / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis*; D007700:Kinetics; D057230:Limit of Detection; D018716:Molecular Mimicry; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry* / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D001560:Benzidines; D005290:Ferric Compounds; D011166:Porphyrins; C000499:ferric oxide; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D010544:Peroxidases; D005947:Glucose
keywords: Colorimetric sensor; One-pot method; Peroxidase; Porphyrin; γ-Fe(2)O(3)