title: The effects of bone pâté on human osteoblasts cell cultures.
abstract: The aim of the present study was to evaluate the effect of bone pate on human osteoblast differentiation by measuring cell viability, alkaline phosphatase activity and expression of the transcription factors and of the major components of the extracellular matrix. Although bone paté has been used in ear surgery for many years and when placed in contact with mastoid and external auditory canal bone become viable, the cellular mechanisms that lead to its osteointegration have never been described. Bone paté taken from four patients subjected to mastoidectomy and affected by middle ear and mastoid cholesteatoma was placed in contact with osteoblast-like cell cultures. Four experimental conditions were obtained: cell cultures treated with bone patè, with bone paté mixed with fibrin glue, with fibrin glue and untreated. After 24 h, the viability of the cells was evaluated; after 1 week, alkaline phosphatase activity and the expression of transcription factors and bone matrix proteins were assessed by quantitative polymerase chain reaction. After 24 h osteoblasts showed increased viability when treated with bone paté (19 % increase) and bone pate mixed with fibrin glue (34 % increase). After 1 week, the number of alkaline phosphatase positive cells increased by 97 and 94 % in cultures treated with bone paté alone and bone pate mixed with fibrin glue. Treatment with bone patè upregulated transcription factors and components of the extracellular matrix. The present data show that bone paté has a high osteoinductive potential on human osteoblasts, enhancing their activity.
authors.affiliation: Section of Otolaryngology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy. nicolaantonioadolfo.quaranta@uniba.it.
authors.forename: Nicola
authors.initials: N
authors.lastname: Quaranta
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Buccoliero
authors.affiliation: Section of Otolaryngology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: De Luca
authors.affiliation: Department of Clinical and Experimental Medicine, University of Foggia, 71100, Foggia, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mori
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy.
authors.forename: Giacomina
authors.initials: G
authors.lastname: Brunetti
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Colucci
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy.
authors.forename: Graziana
authors.initials: G
authors.lastname: Colaianni
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124, Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Grano
mesh_terms: D000368:Aged; D000469:Alkaline Phosphatase / Q000032:analysis; D001842:Bone and Bones; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival; D002675:Child, Preschool; D018424:Cholesteatoma, Middle Ear / Q000601:surgery; D004391:Dust; D004424:Ear Canal / Q000601:surgery; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008416:Mastoid / Q000601:surgery; D008875:Middle Aged; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis; D013506:Otologic Surgical Procedures; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004391:Dust; D015718:Fibrin Tissue Adhesive; D000469:Alkaline Phosphatase
keywords: Bone patè; Cell cultures; Ear surgery; Fibrin glue; Osteoblast; Osteointegration