title: Chemotactic and Phagocytic Activity of Blood Neutrophils in Allergic Asthma.
abstract: Allergic asthma is a chronic inflammatory airway disease, and has been considered a T helper-2-biased response. Studies suggest that neutrophils may be associated with exacerbation and asthma severity. We sought to evaluate the chemotactic activity and phagocytic capacity by peripheral blood neutrophils from individuals with controlled and uncontrolled allergic asthma, and compare the results with non-asthmatic controls groups. Blood neutrophils were isolated from 95 patients: 24 with controlled asthma, 24 uncontrolled asthma, 24 healthy subjects and 23 patients with IgE-mediated allergies other than asthma. The neutrophil chemotaxis, stimulated with LPS, autologous serum or homologous serum, was determined using Boyden chambers. The phagocytic capacity was assessed by ingestion of zimosan particles, and digestion phase was analyzed by NBT test. The phagocytic digestion phase and chemotaxis by neutrophils from asthmatic patients was higher than in non-asthmatic controls (p  < 0.05). Autologous serum-induced neutrophil chemotaxis in patients with uncontrolled asthma was greater (p  < 0.05) than in other study groups. The ingestion phase of phagocytosis showed similar values in asthmatics and non-asthmatics. We conclude that the blood neutrophil from controlled and uncontrolled asthmatic patients exhibit activation markers, particularly phagocytic digestion and chemotactic activities.
authors.affiliation: Santa Casa de São Paulo School of Medical Sciences , São Paulo , Brazil , and.
authors.forename: Tainá
authors.initials: T
authors.lastname: Mosca
authors.forename: Maria C S
authors.initials: MC
authors.lastname: Menezes
authors.forename: Ademir Veras
authors.initials: AV
authors.lastname: Silva
authors.forename: Roberto
authors.initials: R
authors.lastname: Stirbulov
authors.forename: Wilma C N
authors.initials: WC
authors.lastname: Forte
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D001249:Asthma / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D002478:Cells, Cultured; D002633:Chemotaxis; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D003430:Cross-Sectional Studies; D005260:Female; D006636:Histamine Release; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D018927:Anti-Asthmatic Agents; D003176:Complement C3; D003181:Complement C4; D007073:Immunoglobulin E
keywords: Asthma; blood; neutrophils