title: Cep70 regulates microtubule stability by interacting with HDAC6.
abstract: Microtubules, highly dynamic components of the cytoskeleton, are involved in mitosis, cell migration and intracellular trafficking. Our previous work has shown that the centrosomal protein Cep70 regulates microtubule organization and mitotic spindle orientation in mammalian cells. However, it remains elusive whether Cep70 is implicated in microtubule stability. Here we demonstrate that Cep70 enhances microtubule resistance to cold or nocodazole treatment. Our data further show that Cep70 promotes microtubule stability by regulating tubulin acetylation, and plays an important role in stabilizing microtubules. Mechanistic studies reveal that Cep70 interacts and colocalizes with histone deacetylase 6 (HDAC6) in the cytoplasm. These findings suggest that Cep70 promotes microtubule stability by interaction with HDAC6 and regulation of tubulin acetylation.
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing 210096, China. Electronic address: xingjuanshi@seu.edu.cn.
authors.forename: Xingjuan
authors.initials: X
authors.lastname: Shi
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
authors.forename: Yujue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China. Electronic address: dwli@nankai.edu.cn.
authors.forename: Dengwen
authors.initials: D
authors.lastname: Li
mesh_terms: D000107:Acetylation; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003080:Cold Temperature; D057809:HEK293 Cells; D006367:HeLa Cells; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D058105:Polymerization; D011485:Protein Binding; D034741:RNA, Small Interfering; D014404:Tubulin / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases; D008869:Microtubule-Associated Proteins; D015739:Nocodazole; D034741:RNA, Small Interfering; D014404:Tubulin; C574386:CEP70 protein, human; C433811:HDAC6 protein, human
keywords: Cep70; Histone deacetylase 6; Microtubule stability; Tubulin; Tubulin acetylation