title: Scylla serrata reovirus p35 protein expressed in Escherichia coli cells alters membrane permeability.
abstract: To promote viral entry, replication, release, and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes. Scylla serrata reovirus (SsRV) is a major pathogen that can severely damage mud crab (S. serrata) aquaculture. Protein p35, which is encoded by segment 10 of SsRV, contains two transmembrane domains. In this study, we found that SsRV p35 can induce membrane permeability changes when expressed in Escherichia coli. SsRV p35 expressed in bacterial cells existed as monomers under reducing conditions but formed homodimers and homotrimers under non-reducing conditions. These findings demonstrate that p35 may act as a viroporin; further studies are needed to elucidate the detailed structure-function relationships of this protein.
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yuan
authors.forename: Dongyang
authors.initials: D
authors.lastname: Fan
authors.forename: Jifang
authors.initials: J
authors.lastname: Yang
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Jigang
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D003386:Brachyura / Q000821:virology; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects*; D055503:Protein Multimerization; D012087:Reoviridae / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins