title: Neuroglobin protects cardiomyocytes against apoptosis and cardiac hypertrophy induced by isoproterenol in rats.
abstract: Neuroglobin (Ngb) is well known as a physiological role in oxygen homeostasis of neurons and perhaps a protective role against hypoxia and oxidative stress. In this study, we found that Ngb is expressed in rat heart tissues and it is related to isoproterenol induced cardiac hypertrophy. Moreover, overexpression or knock-down of Ngb influences the expression of hypertrophic markers ANP and BNP and the ratio of hypertrophic cells in rat H9c2 myoblasts when isoproterenol treatment. The Annexin V-FITC/PI Staining, Western blot and qPCR analysis showed that the involvement in p53-mediated apoptosis of cardiomyocytes of Ngb is might be the mechanism. This protein could prevent the cells against ROS and POS-induced apoptosis not only in nervous systems but also in cardiomyocytes. From the results, it is concluded that Ngb is a promising protectant in the cardiac hypertrophy, it may be a candidate target to cardiac hypertrophy for clinic treatment.
authors.affiliation: Department of Emergency, Qilu Hospital, Shandong University Jinan, China ; Chest Pain Center, Qilu Hospital, Shandong University Jinan, China ; Key Laboratory of Emergency and Critical Care Medicine of Shandong Province, Qilu Hospital, Shandong University Jinan, China ; Key Laboratory of Cardiovascular Remodeling & Function Research, Chinese Ministry of Education & Chinese Ministry of Public Health, Qilu Hospital, Shandong University Jinan, China ; Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Zhen-Fang
authors.initials: ZF
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Emergency, Xixiang People's Hospital Guangdong Medical College Shenzhen, Guangdong, China.
authors.forename: Yan-Xiang
authors.initials: YX
authors.lastname: Qiao
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Wan-Qun
authors.initials: WQ
authors.lastname: Xu
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Cheng-Tai
authors.initials: CT
authors.lastname: Ma
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Gu
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Zhou
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Chang-Xing
authors.initials: CX
authors.lastname: Cui
authors.affiliation: Department of Emergency, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Di
authors.initials: D
authors.lastname: Xia
authors.affiliation: Department of Emergency, Qilu Hospital, Shandong University Jinan, China ; Chest Pain Center, Qilu Hospital, Shandong University Jinan, China ; Key Laboratory of Emergency and Critical Care Medicine of Shandong Province, Qilu Hospital, Shandong University Jinan, China ; Key Laboratory of Cardiovascular Remodeling & Function Research, Chinese Ministry of Education & Chinese Ministry of Public Health, Qilu Hospital, Shandong University Jinan, China.
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Chen
keywords: Apoptosis; cardiac hypertrophy; cardiomyocytes; oxidative stress; p53