title: Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement.
abstract: The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled using finite elements analysis and experimentally measured using a Microsquisher(®). They are subsequently loaded with preosteoblastic cells, which remain live after 24 and 72 h. The interlockable scaffolds have maintained their ability to fuse with tissue spheroids. This work represents a novel technological platform, enabling the rapid, laser-based, in situ 3D tissue biofabrication.
authors.affiliation: IESL-FORTH, N. Plastira 100, 70013 Heraklion, Greece.
authors.forename: Paulius
authors.initials: P
authors.lastname: Danilevicius
authors.affiliation: Three-Dimensional Technologies Division, Renato Archer Center for Information Technology, Campinas, São Paulo 13069-901, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Rezende
authors.affiliation: Three-Dimensional Technologies Division, Renato Archer Center for Information Technology, Campinas, São Paulo 13069-901, Brazil.
authors.forename: Frederico D A S
authors.initials: FD
authors.lastname: Pereira
authors.affiliation: IESL-FORTH, N. Plastira 100, 70013 Heraklion, Greece.
authors.forename: Alexandros
authors.initials: A
authors.lastname: Selimis
authors.affiliation: Institute of Anatomy and Anthropology, Riga Stradins University, Riga LV-1007, Latvia and Institute of Biomaterials and Biomechanics, Riga Technical University, Riga LV-1048, Latvia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kasyanov
authors.affiliation: Three-Dimensional Technologies Division, Renato Archer Center for Information Technology, Campinas, São Paulo 13069-901, Brazil.
authors.forename: Pedro Y
authors.initials: PY
authors.lastname: Noritomi
authors.affiliation: Three-Dimensional Technologies Division, Renato Archer Center for Information Technology, Campinas, São Paulo 13069-901, Brazil.
authors.forename: Jorge V L
authors.initials: JV
authors.lastname: da Silva
authors.affiliation: IESL-FORTH, N. Plastira 100, 70013 Heraklion, Greece and Department of Materials Science and Technology, University of Crete, Heraklion, 70013, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Chatzinikolaidou
authors.affiliation: IESL-FORTH, N. Plastira 100, 70013 Heraklion, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Farsari
authors.affiliation: IESL-FORTH, N. Plastira 100, 70013 Heraklion, Greece; Three-Dimensional Technologies Division, Renato Archer Center for Information Technology, Campinas, São Paulo 13069-901, Brazil; and DIMAV, INMETRO, Xerem 25250-020, Rio de Janeiro, Brazil.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mironov
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D018914:Cells, Immobilized / Q000502:physiology*; D007834:Lasers; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D058105:Polymerization; D017640:Silicates; D013234:Stem Cells / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D015040:Zirconium
chemical_list: D017640:Silicates; C003784:zircon; D015040:Zirconium