title: Flow-injection chemiluminescence method for the determination of moxifloxacin in pharmaceutical tablets and human urine using silver nanoparticles sensitized calcein-KMnO4 system.
abstract: Silver nanoparticles have been synthesized and were utilized for the enhanced luminometric estimation of moxifloxacin antibiotic. During the experimental procedure, it was clearly found that the addition of silver nanoparticles intensifies the weak chemiluminescence signal intensity of calcein-KMnO4 system by several folds. It was also obvious that the intensity enhancement was linearly proportional to the moxifloxacin concentration and this phenomenon was further utilized for the quantitative determination of target analyte. Effects of the different chemical variables during the experiment were studied to achieve best chemiluminescence signal. Under the optimized experimental parameters, the linear calibration graph was established over the moxifloxacin concentration range of 6.0 × 10(-8) M to 2.5 × 10(-6) M with coefficient of correlation (r (2)) value 0.9998. The lower detection limit was found to be 5.6 × 10(-9) M. The percentage relative standard deviation calculated from five replicate chemiluminescence measurements was found to be 2.63 %. The developed chemiluminescence technique was successfully applied to the determination of moxifloxacin in tablet formulation and spiked human urine sample.
authors.affiliation: Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia, tarabai22@yahoo.com.sg.
authors.forename: Saikh Mohammad
authors.initials: SM
authors.lastname: Wabaidur
authors.forename: Seikh Mafiz
authors.initials: SM
authors.lastname: Alam
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Alothman
authors.forename: Masoom Raza
authors.initials: MR
authors.lastname: Siddiqui
authors.forename: Mu
authors.initials: M
authors.lastname: Naushad
authors.forename: Ayoub A
authors.initials: AA
authors.lastname: Alqadami
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000652:urine; D017022:Flow Injection Analysis / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D024841:Fluoroquinolones / Q000032:analysis* / Q000652:urine; D005960:Glucosides / Q000737:chemistry; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D017895:Manganese Compounds / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D000077266:Moxifloxacin; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods; D016482:Urinalysis / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D005452:Fluoresceins; D024841:Fluoroquinolones; D005960:Glucosides; D017895:Manganese Compounds; D010087:Oxides; D013607:Tablets; C534725:glucopyranosyl 12-hydroxyjasmonic acid; D012834:Silver; C027424:manganese oxide; D000077266:Moxifloxacin; C007740:fluorexon