title: Minimal residual disease of leukemia and the quality of cryopreserved human ovarian tissue in vitro.
abstract: Auto-transplant of cryopreserved ovarian tissue in leukemia patients carries a risk to reintroduce malignant cells. Maturation of ovarian follicles in vitro is a promising strategy to overcome the leukemic cell contamination. The follicle development and survival in 14 cryopreserved ovarian tissues with leukemia-specific PCR marker was evaluated after 7 or 14 days culture. Minimal residual disease (MRD) quantification was assessed by real-time quantitative PCR in order to identify the MRD positive (n = 6) and negative (n = 8) samples and to monitor levels of MRD before and after culture. The morphology of ovarian follicles were studied by light microscopy. After culture, no statistical significant differences were detected in follicle densities between MRD positive- and negative samples. Ovarian MRD either decreased below undetectable or fluctuated near the baseline level after 7 and 14 days in culture. This study provides quantitative in vitro evidence that leukemia contamination does not affect the follicle survival in cryopreserved ovarian tissue.
authors.affiliation: a Women and Children's Division, Oslo University Hospital, Rikshospitalet , Oslo , Norway.|b University of Oslo, Faculty of Medicine , Oslo , Norway.
authors.forename: Babak
authors.initials: B
authors.lastname: Asadi-Azarbaijani
authors.affiliation: c Division of Obstetrics and Gynecology, Karolinska Institute and University Hospital, Huddinge , Stockholm , Sweden.|d Stockholm IVF , Stockholm , Sweden.
authors.forename: Mona
authors.initials: M
authors.lastname: Sheikhi
authors.affiliation: e Departments of Physiology and Pediatrics , University of Turku , Turku , Finland.
authors.forename: Mirja
authors.initials: M
authors.lastname: Nurmio
authors.affiliation: f Tampere University Central Hospital , Tampere , Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Tinkanen
authors.affiliation: g TYKSLAB, Turku University Central Hospital , Turku , Finland.
authors.forename: Vesa
authors.initials: V
authors.lastname: Juvonen
authors.affiliation: h Barts and the London, Queen Mary College, William Harvey Research Institute, Centre for Endocrinology, University of London , London , UK.
authors.forename: Leo
authors.initials: L
authors.lastname: Dunkel
authors.affiliation: c Division of Obstetrics and Gynecology, Karolinska Institute and University Hospital, Huddinge , Stockholm , Sweden.
authors.forename: Outi
authors.initials: O
authors.lastname: Hovatta
authors.affiliation: i Faculty of Veterinary Medicine and Biosciences, University of Oslo , Oslo , Norway.
authors.forename: Irma C
authors.initials: IC
authors.lastname: Oskam
authors.affiliation: j Helsinki University Central Hospital , Helsinki , Finland.|k University of Helsinki , Helsinki , Finland.|l Department of Women's and Children's Health , Karolinska Institute and University Hospital , Stockholm , Sweden.
authors.forename: Kirsi
authors.initials: K
authors.lastname: Jahnukainen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation / Q000379:methods; D005260:Female; D059247:Fertility Preservation / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics; D018365:Neoplasm, Residual / Q000175:diagnosis*; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D006080:Ovarian Follicle; D010053:Ovary; D060888:Real-Time Polymerase Chain Reaction; D046509:Tissue Culture Techniques; D055815:Young Adult
chemical_list: D015514:Oncogene Proteins, Fusion
keywords: Leukemia; minimal residual disease; ovarian tissue; tissue culture