title: [Protective Effect of Activated Nrf2 against Hyperoxia-induced Lung Injury in Neonatal Rats].
abstract: OBJECTIVE
  To observe the effect of nuclear factor erythroid 2-related factor 2 (Nrf2) agonist on the apoptosis of alveolar cell induced by hyperoxia and to explore whether Nrf2 activation could protect neonatal rats from hyperoxia induced lung injury.
  
  
  METHODS
  90 neonatal Sprague-Dawley rats were randomized into room air group (FiO2 =21%, N group), hyperoxia group (0 group) and Nrf2 group (n=30 each). Neonatal rats in the 0 group and Nrf2 group received saline 0. 2 mL and Nrf2 agonist 30 mg/kg respectively at the first and second day after birth, and were exposed in high concentration oxygen (95%) for 4 d. N group rats were fed in room air. The apoptotic index (AI) and Nrf2 expression of lung tissue were detected by TUNEL and immunohistochemistry staining respectively.
  
  
  RESULTS
  Compared with 0 group (28. 8% ± 3. 0%), the AI of alveolar. cell was lower in N group (0. 7%±0. 6%) and Nrf2 group (7. 2% ± 0. 8%) (P<0. 01). The expression of Nrf2 was significantly higher in 0 group (926. 80 ± 130. 51) and Nrf2 group (1038. 40±151. 12) than that in N group (30. 03±9. 99) (P<0. 01).
  
  
  CONCLUSIONS
  Nrf2 activation could reduce the alveolar cellular apoptosis and protect neonatal rats from hyperoxia induced lung injury.
authors.forename: Juan
authors.initials: J
authors.lastname: Ni
authors.forename: Yuan-gui
authors.initials: YG
authors.lastname: Lin
authors.forename: Lan
authors.initials: L
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Jia-fu
authors.initials: JF
authors.lastname: Wei
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Jiang
mesh_terms: D056809:Alveolar Epithelial Cells / Q000166:cytology; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D018496:Hyperoxia; D008168:Lung / Q000187:drug effects* / Q000503:physiopathology; D055370:Lung Injury; D051267:NF-E2-Related Factor 2 / Q000819:agonists*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat