title: Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing.
abstract: Laser speckle contrast imaging (LSCI) shows a great potential for monitoring blood flow, but the spatial resolution suffers from the scattering of tissue. Here, we demonstrate the capability of a combination method of LSCI and skin optical clearing to describe in detail the dynamic response of cutaneous vasculature to vasoactive noradrenaline injection. Moreover, the superior resolution, contrast and sensitivity make it possible to rebuild arteries-veins separation and quantitatively assess the blood flow dynamical changes in terms of flow velocity and vascular diameter at single artery or vein level.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China ; MoE Key Laboratory of Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China ; These authors contributed equally to this work.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Affiliated Hospital, Huazhong University of Science and Technology, Wuhan 430074, China ; These authors contributed equally to this work.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov 410012, Russia ; Institute of Precise Mechanics and Control RAS, Saratov 410028, Russia.
authors.forename: Valery V
authors.initials: VV
authors.lastname: Tuchin
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China ; MoE Key Laboratory of Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
keywords: (120.6150) Speckle imaging; (150.1135) Algorithms; (170.1470) Blood or tissue constituent monitoring; (290.0290) Scattering