title: Interplay of cell dynamics and epithelial tension during morphogenesis of the Drosophila pupal wing.
abstract: How tissue shape emerges from the collective mechanical properties and behavior of individual cells is not understood. We combine experiment and theory to study this problem in the developing wing epithelium of Drosophila. At pupal stages, the wing-hinge contraction contributes to anisotropic tissue flows that reshape the wing blade. Here, we quantitatively account for this wing-blade shape change on the basis of cell divisions, cell rearrangements and cell shape changes. We show that cells both generate and respond to epithelial stresses during this process, and that the nature of this interplay specifies the pattern of junctional network remodeling that changes wing shape. We show that patterned constraints exerted on the tissue by the extracellular matrix are key to force the tissue into the right shape. We present a continuum mechanical model that quantitatively describes the relationship between epithelial stresses and cell dynamics, and how their interplay reshapes the wing.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Etournay
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.
authors.forename: Marko
authors.initials: M
authors.lastname: Popović
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Merkel
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Nandi
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Blasse
authors.affiliation: Institut de Biologie du Développement de Marseille, Marseille, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Aigouy
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Brandl
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Gene
authors.initials: G
authors.lastname: Myers
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Salbreux
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.
authors.forename: Frank
authors.identifier: http://orcid.org/0000-0003-4731-9185
authors.initials: F
authors.lastname: Jülicher
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Eaton
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D004330:Drosophila / Q000196:embryology* / Q000254:growth & development; D004847:Epithelial Cells / Q000502:physiology*; D004848:Epithelium / Q000502:physiology*; D008954:Models, Biological; D011679:Pupa / Q000254:growth & development; D014921:Wings, Animal / Q000196:embryology*
keywords: D. melanogaster; Drosophila; cell biology; cell-dynamics; computational biology; continuum-mechanics; morphogenesis; systems biology; tissue-mechanics; wing-epithelium