title: [Effect of RNA interference of CD59 gene on proliferation of non-small cell lung cancer cell line GLC-P in vitro].
abstract: OBJECTIVE
  To investigate the effect of CD59 gene inhibition mediated by RNA interference on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) GLC-P cells in vitro.
  
  
  METHODS
  Recombinant plasmids for RNA interference of CD59 gene were constructed and transfected into GLC-P cells via lipofectamine 2000. The stably transfected cells were examined with real-time RT-PCR, MTT assay and enzyme-linked immunosorbent assay to investigate the changes in cell proliferation and apoptosis.
  
  
  RESULTS
  Compared with the control cells, the cells transfected with CD59-siRNA showed significantly decreased expression levels of CD59 mRNA (P<0.05) and significantly inhibited cell proliferation.
  
  
  CONCLUSIONS
  CD59 gene is highly expressed in NSCLC and RNA interference-mediated CD59 silencing can strongly inhibit the proliferation and induce apoptosis in GLC-P cells, which shed light on a potentially new target for targeted gene therapy of NSCLC.
authors.affiliation: Department of Cardiothoracic Surgery, Guangzhou Red Cross Hospital/Fourth Affiliated Hospital of Jinan University, Guangzhou 510220, China. E-mail: william0406@163.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Wu
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Wenjin
authors.initials: W
authors.lastname: Hou
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yi
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Wang
authors.forename: Weijia
authors.initials: W
authors.lastname: Lin
mesh_terms: D017209:Apoptosis; D018957:CD59 Antigens / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D015316:Genetic Therapy; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D018957:CD59 Antigens; D012333:RNA, Messenger; D034741:RNA, Small Interfering