title: [Characterization of retrotransposon Bov-B LINE reverse transcriptase gene sequences in parthenogenetic lizards Darevskia unisexualis and bisexual species D. nairensis and D. valentini].
abstract: Cloning and sequencing of the partial reverse transcriptase gene (750 bp) of the Bov-B LINE retrotransposon have been held in parthenogenetic lizards Darevskia unisexualis and its assumed parental bisexual species D. nairensis and D.valentini. It was shown that the percentage of transcriptionally active copies of this gene, which does not contain a stop codon, is almost the same in the three species and is about 75%. The intragenomic divergence level of these sequences is low and was found to be 2.6% in D. unisexualis, 1.9% in D. nairensis, and 1.6% in D. valentini. The phylogenetic analysis shows the distribution of copies of D. unisexualis in each of the two clusters of RT sequences characteristic of D. nairensis and D. valentini. This result supports the view of the hybrid origin of D. unisexualis and does not exclude intraspecific hybridization between D. nairensis and D. valentini.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.|Moscow State Pedagogical University, Moscow, 119991 Russia.|svetlana_godakova@mail.ru.
authors.forename: S A
authors.initials: SA
authors.lastname: Godakova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Korchagin
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: S K
authors.initials: SK
authors.lastname: Semeynova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.|Moscow State Pedagogical University, Moscow, 119991 Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Chernyavskaya
authors.affiliation: Moscow State Pedagogical University, Moscow, 119991 Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Sevast'yanova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Ryskov
mesh_terms: D000818:Animals; D001136:Armenia; D005260:Female; D018628:Gene Dosage; D014644:Genetic Variation; D016678:Genome; D006824:Hybridization, Genetic; D008116:Lizards / Q000145:classification / Q000235:genetics*; D008297:Male; D010312:Parthenogenesis / Q000235:genetics; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D030162:Reptilian Proteins / Q000737:chemistry / Q000235:genetics*; D018626:Retroelements
chemical_list: D030162:Reptilian Proteins; D018626:Retroelements; D012194:RNA-Directed DNA Polymerase
keywords: Bov-B LINE; Darevskia genus; parthenogenetic lizard species; retrotransposon reverse transcriptase (RT) gene; retrotransposons