title: Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells.
abstract: In this study, we developed a novel biosensor based on highly exposed Pt nanoparticles (Pt NPs) decorated porous graphene (PG) for the reliable detection of extracellular hydrogen peroxide (H2O2) released from living cells. The commercially available low-cost hydrophilic CaCO3 spheres were used as template for preparing PG. The porous structure provided larger surface area and more active sites. Due to the porous structure of PG, the Pt NPs supported on PG were not secluded by aggregated graphene layers and were highly exposed to target molecules. Ultrafine Pt NPs were well dispersed and loaded on PG by a method of microwave assistance. Electrochemical performances of the Pt/PG nanocomposites modified glassy carbon electrode (GCE) were investigated. The electrocatalytic reduction of H2O2 showed a wide linear range from 1 to 1477 μM, with a high sensitivity of 341.14 μA mM(-1) cm(-2) and a limit of detection (LOD) as low as 0.50 μM. Moreover, the Pt/PG/GCE exhibited excellent anti-interference property, reproducibility and long-term storage stability. Because of these remarkable analytical advantages, the constructed sensor was used to determine H2O2 released from living cells with satisfactory results. The superior catalytic activity makes Pt/PG nanocomposites a promising candidate for electrochemical sensors and biosensors design.
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China. Electronic address: baoxj133@nenu.edu.cn.
authors.forename: Xiangjie
authors.initials: X
authors.lastname: Bo
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China. Electronic address: guolp078@nenu.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Guo
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D058948:Cell Tracking / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D010984:Platinum / Q000737:chemistry*; D016062:Porosity; D015203:Reproducibility of Results
chemical_list: D010984:Platinum; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Cell tracking; Electrochemical biosensor; Nonenzymatic hydrogen peroxide detection; Porous graphene; Pt nanoparticles