title: In Vitro infection of human dura-mater fibroblasts with Staphylococcus aureus: colonization and reactive production of IL-1beta.
abstract: OBJECTIVE
  Post-operative meningitis, caused mainly by Staphylococcus aureus and Gram-negative rods, is a life-threatening complication after neurosurgery, and its pathogenesis is far from clear. The purpose of this work was to study the experimental infection of human dura-mater fibroblasts and whole human dura by S. aureus.
  
  
  METHODS
  In vitro cultures of human dura-mater fibroblasts and organotypic cultures of small pieces of human dura mater were inoculated with a human-derived S. aureus strain. The pattern of bacterial infection as well as cytokines secretion by the infected fibroblasts was studied.
  
  
  RESULTS
  Our results suggest that colonisation of human dura-mater fibroblasts in culture and whole dura-mater tissue by S. aureus includes bacterial growth on the cell surface, fibroblast intracellular invasion by bacteria and a significant synthesis of interleukin 1beta (IL-1beta) by the infected cells.
  
  
  CONCLUSIONS
  This is the first report of human dura-mater fibroblast infection by S. aureus. Hopefully, these results can lead to a better understanding of the pathogenesis of meningitis caused by this bacterial species and to a more rational therapeutic approach.
authors.forename: Marta A
authors.initials: MA
authors.lastname: Almirón
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Goldschmidt
authors.forename: Andrea Mendoza
authors.initials: AM
authors.lastname: Bertelli
authors.forename: Marisa I
authors.initials: MI
authors.lastname: Gomez
authors.forename: Pablo
authors.initials: P
authors.lastname: Argibay
authors.forename: Norberto A
authors.initials: NA
authors.lastname: Sanjuan
mesh_terms: D002478:Cells, Cultured; D004388:Dura Mater / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D053583:Interleukin-1beta / Q000378:metabolism; D009924:Organ Culture Techniques; D013203:Staphylococcal Infections / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000648:ultrastructure
chemical_list: D053583:Interleukin-1beta
keywords: Culture,; Dura-mater,; Fibroblasts,; Human,; Staphylococcus aureus