title: Distinct effect of xenobiotics on the metal-binding properties of protein molecules.
abstract: The X-ray standing-wave method was applied to study the elemental composition and molecular organization of ordered protein films of alkaline phosphatase exposed to different xenobiotics (drug compounds, lead). Binding of metal ions from triply distilled water to protein molecules has been experimentally observed. Definite differences in the arrangement of impurity metal ions in the films have been established. The considerable enhancement of protein-metal interactions is attributed to partial rearrangement of the protein native structure, induced by xenobiotics.
authors.affiliation: National Research Centre `Kurchatov Institute', Akademika Kurchatova pl. 1, Moscow 123182, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Novikova
authors.affiliation: National Research Centre `Kurchatov Institute', Akademika Kurchatova pl. 1, Moscow 123182, Russia.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kovalchuk
authors.affiliation: Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russia.
authors.forename: Nina
authors.initials: N
authors.lastname: Stepina
authors.affiliation: Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow 119333, Russia.
authors.forename: Radmir
authors.initials: R
authors.lastname: Gaynutdinov
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, GSP-1, Leninskie Gory, Moscow 119991, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Chukhrai
authors.affiliation: Institute of Pediatrics and Pediatric Surgery, Russian Academy of Medical Sciences, Taldomskaya str. 2, Moscow 125412, Russia.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Yurieva
mesh_terms: D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D014965:X-Rays; D015262:Xenobiotics / Q000737:chemistry*
chemical_list: D008670:Metals; D011506:Proteins; D015262:Xenobiotics
keywords: Langmuir–Blodgett monolayer; X-ray fluorescence; X-ray total external reflection; alkaline phosphatase; chelating drug compounds; trace metal binding