title: Sortase A-mediated multi-functionalization of protein nanoparticles.
abstract: We report here a new strategy to enable fast, covalent, and site-directed functionalization of protein nanoparticles using Sortase A-mediated ligation using functional proteins ranging from monomeric to large tetrameric structures. Easy purification of the modified E2 nanoparticles is achieved by functionalization with a thermo-responsive elastin-like-peptide. The resulting protein nanoparticles remained intact and active even after repeated phase transitions, suggesting their use in biocatalysis, biosensing, and imaging applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA. wilfred@udel.edu.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Molino
authors.forename: Szu-Wen
authors.initials: SW
authors.lastname: Wang
authors.forename: Eric T
authors.initials: ET
authors.lastname: Boder
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
mesh_terms: D019881:Aminoacyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D055162:Biocatalysis; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004549:Elastin / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology*; D001616:beta-Galactosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; D002482:Cellulose; D004549:Elastin; D019881:Aminoacyltransferases; C120542:sortase A; D001616:beta-Galactosidase; C074775:CelA endoglucanase; D002480:Cellulase; D003546:Cysteine Endopeptidases