title: Industrial Production of 2,3-Butanediol from the Engineered Corynebacterium glutamicum.
abstract: The platform chemical 2,3-butanediol (2,3-BDO) is a valuable product that can be converted into several petroleum-based chemicals via simple chemical reactions. Here, we produced 2,3-BDO with the non-pathogenic and rapidly growing Corynebacterium glutamicum. To enhance the 2,3-BDO production capacity of C. glutamicum, we introduced budA encoding acetolactate decarboxylase from Klebsiella pneumoniae, a powerful 2,3-BDO producer. Additionally, budB (encoding α-acetolactate synthase) and budC (encoding acetoin reductase) were introduced from K. pneumoniae to reinforce the carbon flux in the 2,3-BDO production. Because budC had a negative effect on 2,3-BDO production in C. glutamicum, the budB and budA introduced strain, SGSC102, was selected for 2,3-BDO production, and batch culture was performed at 30 °C, 250 rpm and pH 6.86 with pure glucose, molasses, and cassava powder as carbon substrates. After batch culture, significant amount of 2,3-BDO (18.9 and 12.0 g/L, respectively) was produced from 80 g/L of pure glucose and cassava powder.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, South Korea.
authors.forename: Jeongmo
authors.initials: J
authors.lastname: Yang
authors.forename: Borim
authors.initials: B
authors.lastname: Kim
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Kim
authors.forename: Yuhyeon
authors.initials: Y
authors.lastname: Kweon
authors.forename: Soojin
authors.initials: S
authors.lastname: Lee
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002072:Butylene Glycols / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000254:growth & development / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D007218:Industrial Microbiology / Q000379:methods*; D055442:Metabolome; D015203:Reproducibility of Results; D013379:Substrate Specificity
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D002244:Carbon