title: Determination of Substitution Patterns of Galactans from Green Seaweeds of the Bryopsidales.
abstract: Sulfated and pyruvylated galactans are the major soluble polysaccharides produced by seaweeds of the Bryopsidales. Their backbones have a complex and variable pattern of substitution which, until now, has only been elucidated for a few species. Methods for determination of sulfate and pyruvic acid content, and chemical strategies to determine their position in the galactan chain are outlined here. These methods can also be applied to other sulfated and/or pyruvylated polysaccharides.
authors.affiliation: Cátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, Buenos Aires, C1417DSE, Argentina.
authors.forename: Paula Ximena
authors.initials: PX
authors.lastname: Arata
authors.forename: Paula Virginia
authors.initials: PV
authors.lastname: Fernández
authors.forename: Marina
authors.initials: M
authors.lastname: Ciancia
mesh_terms: D000460:Chlorophyta / Q000737:chemistry*; D003217:Conductometry / Q000379:methods; D005685:Galactans / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D009391:Nephelometry and Turbidimetry / Q000379:methods; D019289:Pyruvic Acid / Q000032:analysis*; D012624:Seaweed / Q000737:chemistry*; D013431:Sulfates / Q000032:analysis*
chemical_list: D005685:Galactans; D013431:Sulfates; D019289:Pyruvic Acid