title: Blood coagulation and platelet adhesion on polyaniline films.
abstract: Polyaniline is a promising conducting polymer with still increasing application potential in biomedicine. Its surface modification can be an efficient way how to introduce desired functional groups and to control its properties while keeping the bulk characteristics of the material unchanged. The purpose of the study was to synthetize thin films of pristine conducting polyaniline hydrochloride, non-conducting polyaniline base and polyaniline modified with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPSA) and investigate chosen parameters of their hemocompatibility. The modification was performed either by introduction of PAMPSA during the synthesis or by reprotonation of polyaniline base. The polyaniline hydrochloride and polyaniline base had no impact on blood coagulation and platelet adhesion. By contrast, the polyaniline reprotonated with PAMPSA completely hindered coagulation thanks to its interaction with coagulation factors Xa, Va and IIa. The significantly lower platelets adhesion was also found on this surface. Moreover, this film maintains its conductivity at pH of 6, which is an improvement in comparison with standard polyaniline hydrochloride losing most of its conductivity at pH of 4. Polyaniline film with PAMPSA introduced during synthesis had an impact on platelet adhesion but not on coagulation. The combined conductivity, anticoagulation activity, low platelet adhesion and improved conductivity at pH closer to physiological, open up new possibilities for application of polyaniline reprotonated by PAMPSA in blood-contacting devices, such as catheters or blood vessel grafts.
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic; Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic. Electronic address: humpolicek@ft.utb.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Humpolíček
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Kuceková
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic; Department of Fat, Surfactant and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlin, Zlin, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Kašpárková
authors.affiliation: Department of Hematology, Tomas Bata Regional Hospital in Zlin, 762 75 Zlin, Czech Republic; Department of Health Care Sciences, Faculty of Humanities, Tomas Bata University in Zlin, 760 05 Zlin, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Pelková
authors.affiliation: Department of Surface Engineering, Plasma Laboratory, Josef Stefan Institute, 1000 Ljubljana, Slovenia.
authors.forename: Martina
authors.initials: M
authors.lastname: Modic
authors.affiliation: Department of Surface Engineering, Plasma Laboratory, Josef Stefan Institute, 1000 Ljubljana, Slovenia.
authors.forename: Ita
authors.initials: I
authors.lastname: Junkar
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Trchová
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Bober
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Stejskal
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic.
authors.forename: Marián
authors.initials: M
authors.lastname: Lehocký
mesh_terms: D000814:Aniline Compounds; D001777:Blood Coagulation; D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000814:Aniline Compounds; C416807:polyaniline
keywords: Blood coagulation; Hemocompatibility; Platelet adhesion; Poly(2-acrylamido-2-methyl-1-propanesulfonic acid); Polyaniline