title: Microarray analysis of anti-cancer effects of docosahexaenoic acid on human colon cancer model in nude mice.
abstract: Docosahexaenoic acid (DHA), a derivative of ω3- polyunsaturated fatty acids present in fish oil, is well known to have anticancer activity on colon cancer cells, but the molecular and cellular mechanisms remain to be further clarified. In this study, anti-cancer effects of DHA on colon cancer cells were observed in a nude mouse HCT-15 xenograft model. And then, the different genes expression and signal pathways involved in this process were screened and identified using cDNA microarray analysis. Results of genes expression profiles indicated a reprogramming pattern of previously known and unknown genes and transcription factors associated with the action of DHA on colon cancer cells. And several genes related to tumor growth and metastasis including COX2, HIF-1α, VEGF-A, COMP, MMP-1, MMP-9, SCP2, SDC3, which were down-regulated by DHA, were further confirmed in HCT-15 cell line using RT-PCR method. In summary, our data might provide novel information for anti-cancer mechanism of DHA in colon cancer model.
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Shitao
authors.initials: S
authors.lastname: Zou
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Xingjun
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Surgical Oncology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: You
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
authors.affiliation: School of Radiation Medicine and Protection, Medical College of Soochow University Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, University of Oklahoma Health Science Center Oklahoma City, Oklahoma, USA.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Ding
authors.affiliation: Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Jinchang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China ; Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital Suzhou 215001, Jiangsu, P. R. China.
authors.forename: Jundong
authors.initials: J
authors.lastname: Zhou
keywords: DHA; colon cancer; in vivo; microarray analysis; nude mice