title: Polymorphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation.
abstract: Individual sensitivity to ionising radiation (IR) is the result of interaction between exposure, DNA damage, and its repair, which is why polymorphisms in DNA repair genes could play an important role. We examined the association between DNA damage, expressed as micronuclei (MNi), nuclear buds (NBs), and nucleoplasmic bridges (NPBs) and single nucleotide polymorphisms in selected DNA repair genes (APE1, hOGG1, XRCC1, XRCC3, XPD, PARP1, MGMT genes; representative of the different DNA repair pathways operating in mammals) in 77 hospital workers chronically exposed to low doses of IR, and 70 matched controls. A significantly higher MNi frequency was found in the exposed group (16.2±10.4 vs. 11.5±9.4; P=0.003) and the effect appeared to be independent from the principal confounding factor. Exposed individuals with hOGG1, XRCC1, PARP1, and MGMT wild-type alleles or APEX1, as well as XPD (rs13181) heterozygous showed a significantly higher MNi frequency than controls with the same genotypes. Genetic polymorphism analysis and cytogenetic dosimetry have proven to be a powerful tool complementary to physical dosimetry in regular health surveillance programmes.
authors.forename: Mirta
authors.initials: M
authors.lastname: Milić
authors.forename: Ružica
authors.initials: R
authors.lastname: Rozgaj
authors.forename: Vilena
authors.initials: V
authors.lastname: Kašuba
authors.forename: Ana-Marija
authors.initials: AM
authors.lastname: Jazbec
authors.forename: Boris
authors.initials: B
authors.lastname: Starčević
authors.forename: Barnaba
authors.initials: B
authors.lastname: Lyzbicki
authors.forename: Gloria
authors.initials: G
authors.lastname: Ravegnini
authors.forename: Corrado
authors.initials: C
authors.lastname: Zenesini
authors.forename: Muriel
authors.initials: M
authors.lastname: Musti
authors.forename: Patrizia
authors.initials: P
authors.lastname: Hrelia
authors.forename: Sabrina
authors.initials: S
authors.lastname: Angelini
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D048749:Cytokinesis / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics / Q000276:immunology* / Q000528:radiation effects*; D005260:Female; D006282:Health Personnel; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects; D020641:Polymorphism, Single Nucleotide; D000069079:Radiation Exposure / Q000009:adverse effects*; D011839:Radiation, Ionizing; D055815:Young Adult
chemical_list: D015415:Biomarkers