title: Collective and individual glycolytic oscillations in yeast cells encapsulated in alginate microparticles.
abstract: Yeast cells were encapsulated into alginate microparticles of a few hundred micrometers diameter using a centrifuge-based droplet shooting device. We demonstrate the first experimental results of glycolytic oscillations in individual yeast cells immobilized in this way. We investigated both the individual and collective oscillatory behaviors at different cell densities. As the cell density increased, the amplitude of the individual oscillations increased while their period decreased, and the collective oscillations became more synchronized, with an order parameter close to 1 (indicating high synchrony). We also synthesized biphasic-Janus microparticles encapsulating yeast cells of different densities in each hemisphere. The cellular oscillations between the two hemispheres were entrained at both the individual and population levels. Such systems of cells encapsulated into microparticles are useful for investigating how cell-to-cell communication depends on the density and spatial distribution of cells.
authors.affiliation: Graduate School of Environment and Information Sciences, Yokohama National University (YNU), 79-7 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Amemiya
authors.affiliation: Graduate School of Environment and Information Sciences, Yokohama National University (YNU), 79-7 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Obase
authors.affiliation: Graduate School of Environment and Information Sciences, Yokohama National University (YNU), 79-7 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Hiramatsu
authors.affiliation: Graduate School of Environment and Information Sciences, Yokohama National University (YNU), 79-7 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan.
authors.forename: Kiminori
authors.initials: K
authors.lastname: Itoh
authors.affiliation: Research Center for Life and Environmental Sciences, Toyo University, 1-1-1 Izumino, Itakura, Ora, Gunma 374-0193, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shibata
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuda-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takinoue
authors.affiliation: Tokyo Metropolitan College of Industrial Technology (TMCIT), 1-10-40 Higashinoshi, Shinagawa-ku, Tokyo 140-0011, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001683:Biological Clocks; D018914:Cells, Immobilized / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006019:Glycolysis; D006603:Hexuronic Acids / Q000737:chemistry; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid