title: A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures.
abstract: Despite exciting new possibilities for regenerative therapy posed by the ability to induce pluripotent stem cells, recapitulation of three-dimensional kidneys for repair or replacement has not been possible. ARID3a-deficient mouse tissues generated multipotent, developmentally plastic cells. Therefore, we assessed the adult mouse ARID3a-/- kidney cell line, KKPS5, which expresses renal progenitor surface markers as an alternative cell source for modeling kidney development. Remarkably, these cells spontaneously developed into multicellular nephron-like structures in vitro, and engrafted into immunocompromised medaka mesonephros, where they formed mouse nephron structures. These data implicate KKPS5 cells as a new model system for studying kidney development.
authors.affiliation: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.|Immunobiology and Cancer Research, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.|Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Carol F
authors.initials: CF
authors.lastname: Webb
authors.affiliation: Immunobiology and Cancer Research, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Ratliff
authors.affiliation: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Powell
authors.affiliation: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Celeste R
authors.initials: CR
authors.lastname: Wirsig-Wiechmann
authors.affiliation: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Lakiza
authors.affiliation: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Obara
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D007668:Kidney / Q000033:anatomy & histology* / Q000166:cytology; D051379:Mice; D018345:Mice, Knockout; D009990:Oryzias; D013234:Stem Cells / Q000166:cytology; D014157:Transcription Factors / Q000235:genetics
chemical_list: C097532:Arid3a protein, mouse; D004268:DNA-Binding Proteins; D014157:Transcription Factors
keywords: ARID3a; Medaka; Nephron structure; Renal progenitor