title: Diversity of endophytic fungi in Glycine max.
abstract: Endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. With the isolation and identification of these fungi, new species are being discovered, and ecological relationships with their hosts have also been studied. In Glycine max, limited studies have investigated the isolation and distribution of endophytic fungi throughout leaves and roots. The distribution of these fungi in various plant organs differs in diversity and abundance, even when analyzed using molecular techniques that can evaluate fungal communities in different parts of the plants, such as denaturing gradient gel electrophoresis (DGGE). Our results show there is greater species richness of culturable endophytic filamentous fungi in the leaves G. max as compared to roots. Additionally, the leaves had high values for diversity indices, i.e. Simpsons, Shannon and Equitability. Conversely, dominance index was higher in roots as compared to leaves. The fungi Ampelomyces sp., Cladosporium cladosporioides, Colletotrichum gloeosporioides, Diaporthe helianthi, Guignardia mangiferae and Phoma sp. were more frequently isolated from the leaves, whereas the fungi Fusarium oxysporum, Fusarium solani and Fusarium sp. were prevalent in the roots. However, by evaluating the two communities by DGGE, we concluded that the species richness was higher in the roots than in the leaves. UPGMA analysis showed consistent clustering of isolates; however, the fungus Leptospora rubella, which belongs to the order Dothideales, was grouped among species of the order Pleosporales. The presence of endophytic Fusarium species in G. max roots is unsurprising, since Fusarium spp. isolates have been previously described as endophyte in other reports. However, it remains to be determined whether the G. max Fusarium endophytes are latent pathogens or non-pathogenic forms that benefit the plant. This study provides a broader knowledge of the distribution of the fungal community in G. max leaves and roots, and identifies the genetic relationships among the isolated species.
authors.affiliation: Departamento de Microbiologia - Bioagro, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, CEP 36570 900 Viçosa, MG, Brazil.
authors.forename: Elio Gomes
authors.initials: EG
authors.lastname: Fernandes
authors.affiliation: Departamento de Fitopatologia, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, CEP 36570 900 Viçosa, MG, Brazil.
authors.forename: Olinto Liparini
authors.initials: OL
authors.lastname: Pereira
authors.affiliation: Departamento de Microbiologia - Bioagro, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, CEP 36570 900 Viçosa, MG, Brazil.
authors.forename: Cynthia Cânedo
authors.initials: CC
authors.lastname: da Silva
authors.affiliation: Departamento de Microbiologia - Bioagro, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, CEP 36570 900 Viçosa, MG, Brazil.
authors.forename: Claudia Braga Pereira
authors.initials: CB
authors.lastname: Bento
authors.affiliation: Departamento de Microbiologia - Bioagro, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, CEP 36570 900 Viçosa, MG, Brazil. Electronic address: mvqueiro@ufv.br.
authors.forename: Marisa Vieira
authors.initials: MV
authors.lastname: de Queiroz
mesh_terms: D001483:Base Sequence; D044822:Biodiversity; D001938:Brazil; D004275:DNA, Ribosomal; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D060026:Endophytes / Q000145:classification* / Q000302:isolation & purification; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D013025:Glycine max / Q000382:microbiology*
chemical_list: D004275:DNA, Ribosomal
keywords: Clustering analysis; DGGE; Fungal community; ITS; Phytopathogenic fungi