title: Effects of Bush Sophora Root polysaccharide and its sulfate on immuno-enhancing of the therapeutic DVH.
abstract: Bush Sophora Root polysaccharide (BSRPS) and its sulfate, sulfated Bush Sophora Root polysaccharide (sBSRPS), possess the antiviral activities against duck hepatitis A virus. However their antiviral mechanisms are still not clear. This paper reported their immuno-enhancing roles in the therapeutic effects for duck virus hepatitis (DVH). The effects of BSRPS and sBSRPS on stimulating lymphocyte proliferation were investigated by MTT methods. After that, ducklings were challenged with DHAV and treated with BSRPS and sBSRPS. Meanwhile, the total antibody (Ab), cytokines including interferon gamma (IFN-γ), hepatocyte growth factor (HGF), interleukin (IL)-2, IL-6 and IL-8 were determined by enzyme-linked immuno sorbent assay methods. The results showed that BSRPS owned a fine hepatoprotective effect with stable HGF producing ability. Sulfated modification was able to increase the proliferation rates of B and T lymphocytes and the secretions of total Ab, IFN-γ and IL-2, as comparison with those of BSRPS group. In summary, both of them exhibited immuno-enhancing effects on the therapeutic effects for DVH, and the capacity of sBSRPS was stronger than that of BSRPS.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Luanting
authors.initials: L
authors.lastname: Xian
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Meiyun
authors.initials: M
authors.lastname: Song
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: liujiaguo@njau.edu.cn.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Sun
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D004372:Ducks; D004371:Hepatitis Virus, Duck / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy*; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010850:Picornaviridae Infections / Q000188:drug therapy*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D029909:Sophora / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides; D013431:Sulfates
keywords: Bush Sophora Root polysaccharide; Duck virus hepatitis; Immuno-enhancing