title: [Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells].
abstract: OBJECTIVE
  To express solute carrier 26A proteins in HEK-293 cells and explore their functions.
  
  
  METHODS
  SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing SLC26A proteins was measured by whole-cell patch recording.
  
  
  RESULTS
  All the SLC26A transporters were expressed on the membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which represented their binding capability with anions.
  
  
  CONCLUSIONS
  The SLC26A transporters expressed on HEK cells show similar functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A transporters.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. E-mail: chaofan1217@yeah.net.
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
authors.forename: Mingyu
authors.initials: M
authors.lastname: Fu
authors.forename: Zhongju
authors.initials: Z
authors.lastname: Xiao
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
mesh_terms: D027321:Anion Transport Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D018408:Patch-Clamp Techniques; D000076988:Sulfate Transporters; D014162:Transfection
chemical_list: D027321:Anion Transport Proteins; C473560:SLC26A1 protein, human; D000076988:Sulfate Transporters