title: Genomic cloning, expression, and single nucleotide polymorphism association analysis of the insulin-like androgenic gland hormone gene in the oriental river prawn (Macrobrachium nipponense).
abstract: Increasing evidence suggests that the insulin-like androgenic gland hormone (IAG) gene plays an important role in male sexual differentiation, metabolism, and growth in crustaceans. In the present study, we isolated the full-length genome sequence of IAG by genome walking based on the cDNA sequence in Macrobrachium nipponense. Four novel single nucleotide polymorphisms (SNPs) were studied, including 509G>T, 529G>T, 590A>T in intron 1, and 2226A>G in intron 2. The association of genetic variation with growth traits [body length (BL) and body weight (BW)] was analyzed. Individuals with GG geno- type at locus 2226A>G maintained higher mean BL (P < 0.01) and BW (P < 0.05) than AA and GA individuals. These results suggest that IAG SNPs may be useful molecular markers for selecting growth traits in M. nipponense.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: F J
authors.initials: FJ
authors.lastname: Li
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: F W
authors.initials: FW
authors.lastname: Jiang
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: H K
authors.initials: HK
authors.lastname: Bai
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China fuht@ffrc.cn.
authors.forename: H T
authors.initials: HT
authors.lastname: Fu
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: S B
authors.initials: SB
authors.lastname: Jin
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Sun
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: H
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhang
mesh_terms: D000728:Androgens / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D056726:Genetic Association Studies; D042341:Gonadal Hormones / Q000096:biosynthesis / Q000235:genetics*; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D008297:Male; D033541:Palaemonidae / Q000235:genetics / Q000254:growth & development; D020641:Polymorphism, Single Nucleotide; D012733:Sex Differentiation / Q000235:genetics*
chemical_list: D000728:Androgens; D042341:Gonadal Hormones; D007328:Insulin