title: LbL Assembly of Albumin on Nitric Oxide-Releasing Silica Nanoparticles Using Suramin, a Polyanion Drug, as an Interlayer Linker.
abstract: Preformed protein corona of nanoparticles can be utilized as a promising formulation strategy for improving nano drug delivery. Nitric oxide (NO) is a labile molecule with extensive therapeutic implications. In this study, we test whether preformation of protein coatings can enhance the performance of NO-delivering nanoparticles. S-Nitroso (SNO) silica nanoparticles (SNO-SiNPs) were prepared using a nanoprecipitation method. For the first time, bovine serum albumin (BSA) was used to coat NO-releasing nanoparticles, facilitated by a polyanionic drug, suramin, under a layer-by-layer (LbL) scheme. Bare and coated nanoparticles were characterized by zeta-potential, size, and spectroscopic measurements. We demonstrate that albumin/suramin-surface coassembly has advantages in preventing particle aggregation during lyophilization, controlling NO release and exerting an enhanced anticancer effect.
authors.affiliation: †School of Pharmacy, National Defense Medical Center, Taipei 11490, Taiwan, Republic of China.
authors.forename: Hung-Chang
authors.initials: HC
authors.lastname: Chou
authors.affiliation: ‡School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan, Republic of China.
authors.forename: Shih-Jiuan
authors.initials: SJ
authors.lastname: Chiu
authors.affiliation: †School of Pharmacy, National Defense Medical Center, Taipei 11490, Taiwan, Republic of China.
authors.forename: Teh-Min
authors.initials: TM
authors.lastname: Hu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000627:therapeutic use; D002417:Cattle; D016503:Drug Delivery Systems; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D009569:Nitric Oxide / Q000737:chemistry* / Q000627:therapeutic use; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013498:Suramin / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D009569:Nitric Oxide; D000071228:Polyelectrolytes; D011108:Polymers; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D013498:Suramin