title: [Effects of Silencing AKT Gene by shRNA on Proliferation, Apoptosis and Notch1 Signal Pathway in Jeko-1 Cells].
abstract: OBJECTIVE
  To investigate the effects of silencing AKT gene by RNA interference on the proliferation, apoptosis and the expression of Notch1 signal pathway-related proteins in mantle cell lymphoma Jeko-1 cell line.
  
  
  METHODS
  The hairpin-like oligonucleotide sequences targeting AKT gene were designed and transfected into Jeko-1 cells by lipofectamine(TM) 2000. The AKT mRNA and protein were detected by RQ-PCR and Western blot respectively. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expression of BCL-2, BAX, caspase-3, caspase-9, p-AKT, Notch1, HES1 was detected by Western blot.
  
  
  RESULTS
  AKT mRNA was markedly suppressed by the shRNA targeting AKT. AKT shRNA suppressed the proliferation of Jeko-1 cells and induced apoptosis of these cells. The cell apoptotic rates were (37.72±4.39)%, (2.62±1.53)%, (1.57±0.42)% in AKT shRNA, Neg-shRNA and Control, respectively, The difference between them was statistically significant (P<0.01). AKT shRNA down-regulated the expression of Bcl-2, AKT p-AKT , Notch1, and HES1, up-regulated the expression of BAX, caspase-3, caspase-9.
  
  
  CONCLUSIONS
  Silencing AKT gene can inhibit the proliferation of Jeko-1 cells line, induc cell apoptosis, its mechanism may be associated with specially inhibiting PI3K/AKT signaling pathway and down-regulating the activity of Notch1 signaling pathway.
authors.affiliation: Department of Laboratorial Examination, The First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China. E-mail: nchuangyiqun@126.com.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D053453:Caspase 9; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D020868:Gene Silencing; D006801:Humans; D020522:Lymphoma, Mantle-Cell; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051881:Receptor, Notch1; D015398:Signal Transduction; D014162:Transfection
chemical_list: C497761:NOTCH1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051881:Receptor, Notch1; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9