title: Antagonism effects of cypermethrin on interleukin-6-induced androgen receptor activation.
abstract: To identify whether androgen receptor (AR) antagonism by cypermethrin involves interleukin-6 (IL-6)-induced ligand-independent AR signaling, we have developed the AR reporter gene assay. The reporter gene plasmid pMMTV-chloramphenicol transferase (CAT) was transfected into LNCaP cells. IL-6 increased expression of MMTV-CAT significantly (P<0.05). Cypermethrin decreased CAT reporter expression induced by IL-6 (50 ng/ml), and the significant inhibition was detected at 10(-5)M (P<0.05). IL-6 induces ligand-independent activation of AR. Cypermethrin exhibits inhibitory effects on IL-6-induced ligand-independent AR signaling. We provide a novel insight into cypermethrin-mediated antagonism of the IL-6-mediated ligand-independent activation of the AR.
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Xu
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Zhou
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhou
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ju
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Pan
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Zhang
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Mei-Rong
authors.initials: MR
authors.lastname: Zhang
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China.
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Yu
authors.affiliation: School of Public Health, Xuzhou Medical College, 209 Tong-Shan Road, Xuzhou, Jiangsu 221002, China. Electronic address: lichunxu2002@163.com.
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Xu
mesh_terms: D000818:Animals; D002374:Catalase / Q000235:genetics; D002460:Cell Line; D013196:Dihydrotestosterone / Q000494:pharmacology; D017930:Genes, Reporter; D015850:Interleukin-6 / Q000494:pharmacology*; D011722:Pyrethrins / Q000633:toxicity*; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D011722:Pyrethrins; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; C017160:cypermethrin; D002374:Catalase
keywords: Androgen receptor; Antiandrogen; Cypermethrin; Interleukin-6; Ligand-independent manner