title: Characterization of a feruloyl esterase B from Talaromyces cellulolyticus.
abstract: A feruloyl esterase catalyzes the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from esterified sugars in plant cell walls. Talaromyces cellulolyticus is a high cellulolytic-enzyme producing fungus. However, there is no report for feruloyl esterase activity of T. cellulolyticus. Analysis of the genome database of T. cellulolyticus identified a gene encoding a putative feruloyl esterase B. The recombinant enzyme was prepared using a T. cellulolyticus homologous expression system and characterized. The purified enzyme exhibited hydrolytic activity toward p-nitrophenyl acetate, p-nitrophenyl trans-ferulate, methyl ferulate, rice husk, and bagasse. HPLC assays showed that the enzyme released ferulic acid and p-coumaric acid from hydrothermal-treated rice husk and bagasse. Trichoderma sp. is well-known high cellulolytic-enzyme producing fungus useful for the lignocellulosic biomass saccharification. Interestingly, no feruloyl esterase has been reported from Trichoderma sp. The results show that this enzyme is expected to be industrially useful for biomass saccharification.
authors.affiliation: a Biomass Refinery Research Center , National Institute of Advanced Industrial Science and Technology (AIST) , Higashi-hiroshima , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: b Frontier Research Labs , Institute for Innovation Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Yoshida
authors.affiliation: b Frontier Research Labs , Institute for Innovation Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Fukada
authors.affiliation: a Biomass Refinery Research Center , National Institute of Advanced Industrial Science and Technology (AIST) , Higashi-hiroshima , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.affiliation: b Frontier Research Labs , Institute for Innovation Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Tokura
authors.affiliation: a Biomass Refinery Research Center , National Institute of Advanced Industrial Science and Technology (AIST) , Higashi-hiroshima , Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishikawa
mesh_terms: D002109:Caffeic Acids / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D003373:Coumaric Acids / Q000737:chemistry; D006868:Hydrolysis; D032901:Talaromyces / Q000737:chemistry / Q000201:enzymology*
chemical_list: D002109:Caffeic Acids; D002241:Carbohydrates; D003373:Coumaric Acids; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; C530806:methyl ferulate
keywords: bagasse; biomass; cellulase; fungus; saccharification