title: Impact of the substrate loading regime and phosphoric acid supplementation on performance of biogas reactors and microbial community dynamics during anaerobic digestion of chicken wastes.
abstract: This study evaluates the effects of increasing organic loading rate (OLR) and decreasing hydraulic retention time (HRT) as well as phosphoric acid addition on mesophilic reactors' performance and biogas production from chicken wastes. Furthermore, microbial community composition in reactors was characterized by a 16S rRNA gene-based pyrosequencing analysis. Each step of increasing OLR impacted on the activity of microorganisms what caused a temporary decrease in biogas production. The addition of phosphoric acid resulted in the increased biogas production with values between 361 and 447 mL g(VS)(-1) from day 61 to day 74 compared to control reactor (309-350 mL g(VS)(-1)). With reactors' operation, Bacteroidetes phylotypes were noticeably replaced with Firmicutes representatives, and significant increase of Clostridium sp. was identified. Within Euryarchaeota, Methanosarcina sp. dominated in all analyzed samples, in which high ammonium levels were detected (3.4-4.9 NH4(+)-N g L(-1)). These results can help in better understanding the anaerobic digestion process of simultaneously ammonium/phosphate-rich substrates.
authors.affiliation: Department of Technologies, A.E. Arbuzov Institute of Organic and Physical Chemistry, RAN, Kazan 420088, The Republic of Tatarstan, Russia.
authors.forename: Dmitry E
authors.initials: DE
authors.lastname: Belostotskiy
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, Kazan 420008, The Republic of Tatarstan, Russia.
authors.forename: Elvira E
authors.initials: EE
authors.lastname: Ziganshina
authors.affiliation: Laboratory of Omics Technologies, Kazan (Volga Region) Federal University, Kazan 420008, The Republic of Tatarstan, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Siniagina
authors.affiliation: Laboratory of Omics Technologies, Kazan (Volga Region) Federal University, Kazan 420008, The Republic of Tatarstan, Russia.
authors.forename: Eugenia A
authors.initials: EA
authors.lastname: Boulygina
authors.affiliation: Department of Technologies, A.E. Arbuzov Institute of Organic and Physical Chemistry, RAN, Kazan 420088, The Republic of Tatarstan, Russia.
authors.forename: Vasili A
authors.initials: VA
authors.lastname: Miluykov
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, Kazan 420008, The Republic of Tatarstan, Russia. Electronic address: a.ziganshin06@fulbrightmail.org.
authors.forename: Ayrat M
authors.initials: AM
authors.lastname: Ziganshin
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D000693:Anaerobiosis / Q000502:physiology; D000818:Animals; D041963:Bacteroidetes / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D003013:Clostridium / Q000378:metabolism; D000068536:Bacillota / Q000378:metabolism; D017020:Methanosarcina / Q000378:metabolism; D010756:Phosphoric Acids / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000379:methods
chemical_list: D064751:Ammonium Compounds; D056804:Biofuels; D010756:Phosphoric Acids; D012336:RNA, Ribosomal, 16S; C030242:phosphoric acid
keywords: Ammonia inhibition; Biogas; Chicken wastes; Microbial diversity; Phosphoric acid