title: Probe-free real-time reverse transcription polymerase chain reaction assays for the detection and typing of porcine reproductive and respiratory syndrome virus in Canada.
abstract: Porcine reproductive and respiratory syndrome (PRRS) has tremendous impact on the pork industry in North America. The molecular diagnosis of infection with PRRS virus (PRRSV) is hampered by its considerable strain diversity. In this study, 43 previously published or newly developed primers for probe-free real-time reverse transcription polymerase chain reaction (RT-PCR) were evaluated on their sensitivity, specificity, reproducibility, and repeatability, using a diverse panel of 36 PRRSV strains as well as other arteriviruses and unrelated porcine viruses. Three primer pairs had excellent diagnostic and analytical sensitivity on par with a probe-based reference assay, absolute specificity to virus genotype and species, as well as over 95% reproducibility and repeatability across a wide dynamic range.
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta (Eschbaumer, Li, Czub); Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany (Wernike); and Marshall Swine and Poultry Health Services, Camrose, Alberta (Marshall).
authors.forename: Michael
authors.initials: M
authors.lastname: Eschbaumer
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta (Eschbaumer, Li, Czub); Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany (Wernike); and Marshall Swine and Poultry Health Services, Camrose, Alberta (Marshall).
authors.forename: Wansi May
authors.initials: WM
authors.lastname: Li
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta (Eschbaumer, Li, Czub); Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany (Wernike); and Marshall Swine and Poultry Health Services, Camrose, Alberta (Marshall).
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wernike
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta (Eschbaumer, Li, Czub); Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany (Wernike); and Marshall Swine and Poultry Health Services, Camrose, Alberta (Marshall).
authors.forename: Frank
authors.initials: F
authors.lastname: Marshall
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta (Eschbaumer, Li, Czub); Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany (Wernike); and Marshall Swine and Poultry Health Services, Camrose, Alberta (Marshall).
authors.forename: Markus
authors.initials: M
authors.lastname: Czub
mesh_terms: D000818:Animals; D001483:Base Sequence; D002170:Canada / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D004279:DNA, Viral