title: Involvement of NRF2 in Perfluorooctanoic Acid-Induced Testicular Damage in Male Mice.
abstract: Perfluorooctane acid (PFOA) is a hazardous environmental pollutant that has been reported to exert adverse effects on animal and human health. In this study, male mice were orally administered different concentrations of PFOA (2.5, 5, or 10 mg/kg/day) to evaluate the reproductive toxicity. Exposure to PFOA for 14 consecutive days obviously disrupted seminiferous tubules and reduced sperm count. The highest concentration of PFOA (10 mg/kg/day) caused growth retardation and diminished absolute testis weight. Furthermore, PFOA treatment significantly increased the generation of oxidative stress indicators malondialdehyde and hydrogen peroxide, decreased the expression of transcription factor NRF2, and inhibited the activities of antioxidant enzymes superoxide dismutase and catalase in the testis. Moreover, PFOA exposure up-regulated p-p53 and BAX expression and down-regulated BCL-2 expression in the testis. These results indicated that PFOA-induced male reproductive disorders might be involved in developmental impairment and inhibition of NRF2-mediated antioxidant response in the testis of mice.
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Zou
authors.affiliation: Nanchang University Press, Nanchang, PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zou
authors.affiliation: Library, Nanchang University, Nanchang, PR China.
authors.forename: Fangming
authors.initials: F
authors.lastname: Liu
authors.affiliation: Library, Nanchang University, Nanchang, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xia
authors.affiliation: Library, Nanchang University, Nanchang, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, PR China zhangdalei@ncu.edu.cn.
authors.forename: Dalei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002210:Caprylates / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D019254:Genes, bcl-2 / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D008297:Male; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000187:drug effects* / Q000235:genetics*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D012671:Seminiferous Tubules / Q000187:drug effects; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects / Q000235:genetics; D013733:Testicular Diseases / Q000139:chemically induced* / Q000235:genetics*; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000975:Antioxidants; C494827:Bax protein, mouse; D002210:Caprylates; D005466:Fluorocarbons; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D051028:bcl-2-Associated X Protein; C023036:perfluorooctanoic acid
keywords: developmental impairment; oxidative stress; perfluorooctane acid; reproductive toxicity; spermatogenesis