title: Poly-3-hydroxybutyrate (PHB) production from alkylphenols, mono and poly-aromatic hydrocarbons using Bacillus sp. CYR1: A new strategy for wealth from waste.
abstract: In the present study five different types of alkylphenols, each of the two different types of mono and poly-aromatic hydrocarbons were selected for degradation, and conversion into poly-3-hydroxybutyrate (PHB) using the Bacillus sp. CYR1. Strain CYR1 showed growth with various toxic organic compounds. Degradation pattern of all the organic compounds at 100 mg/l concentration with or without addition of tween-80 were analyzed using high pressure liquid chromatography (HPLC). Strain CYR1 showed good removal of compounds in the presence of tween-80 within 3 days, but it took 6 days without addition of tween-80. Strain CYR1 showed highest PHB production with phenol (51 ± 5%), naphthalene (42 ± 4%), 4-chlorophenol (32 ± 3%) and 4-nonylphenol (29 ± 3%). The functional groups, structure, and thermal properties of the produced PHB were analyzed. These results denoted that the strain Bacillus sp. CYR1 can be used for conversion of different toxic compounds persistent in wastewaters into useable biological polyesters.
authors.affiliation: Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran 050-8585, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Venkateswar Reddy
authors.affiliation: Research Center for Environmentally Friendly Materials Engineering, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran, Hokkaido 050-8585, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Mawatari
authors.affiliation: Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto-shi, Kyoto 606-8501, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yajima
authors.affiliation: Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran 050-8585, Japan.
authors.forename: Chigusa
authors.initials: C
authors.lastname: Seki
authors.affiliation: Biomass Refinery Research Center, National Institute of Advanced Industrial, Sciences and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; Bioproduction Research Institute, National Institute of Advanced Industrial Sciences, and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Hoshino
authors.affiliation: Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran 050-8585, Japan. Electronic address: ychang@mmm.muroran-it.ac.jp.
authors.forename: Young-Cheol
authors.initials: YC
authors.lastname: Chang
mesh_terms: D000478:Alkylation; D001407:Bacillus / Q000145:classification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006885:Hydroxybutyrates / Q000302:isolation & purification / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control; D010636:Phenols / Q000302:isolation & purification / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification / Q000378:metabolism*; D011091:Polyesters / Q000302:isolation & purification / Q000378:metabolism*; D059027:Recycling / Q000379:methods; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism; D018508:Water Purification / Q000379:methods; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D007220:Industrial Waste; D010636:Phenols; D011084:Polycyclic Aromatic Hydrocarbons; D011091:Polyesters; D014874:Water Pollutants, Chemical; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Alkylphenols; Aromatic compounds; Bacillus sp. CYR1; Poly-3-hydroxybutyrate (PHB); Transmission electron microscopy (TEM)