title: A first insight into the spleen transcriptome of the notothenioid fish Lepidonotothen nudifrons: Resource description and functional overview.
abstract: In this study, we describe a de novo sequencing and assembly of the spleen transcriptome of Lepidonotothen nudifrons, a notothenioid fish widely distributed around the Antarctic Peninsula and the Scotia Arc. Sequences were generated on an Illumina MiSeq system and assembled to a total of 112,477 transcripts. Putative functional annotation was possible for more than 34% of the transcripts. This data will be relevant for future studies targeting the erythrocyte turnover, oxygen transport mechanism and immune system, which are key functional traits to investigate cold adaptation and thermal sensitivity of Antarctic notothenioids.
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: chiara.papetti@gmail.com.
authors.forename: Chiara
authors.initials: C
authors.lastname: Papetti
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: lars.harms@awi.de.
authors.forename: Lars
authors.initials: L
authors.lastname: Harms
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: heidrun.windisch@hhu.de.
authors.forename: Heidrun Sigrid
authors.initials: HS
authors.lastname: Windisch
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: stephan.frickenhaus@awi.de.
authors.forename: Stephan
authors.initials: S
authors.lastname: Frickenhaus
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: tina.sandersfeld@awi.de.
authors.forename: Tina
authors.initials: T
authors.lastname: Sandersfeld
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: jutta.juergens@awi.de.
authors.forename: Jutta
authors.initials: J
authors.lastname: Jürgens
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: nils.koschnick@awi.de.
authors.forename: Nils
authors.initials: N
authors.lastname: Koschnick
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: rainer.knust@awi.de.
authors.forename: Rainer
authors.initials: R
authors.lastname: Knust
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: hans.poertner@awi.de.
authors.forename: Hans-Otto
authors.initials: HO
authors.lastname: Pörtner
authors.affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, Bremerhaven 27570, Germany. Electronic address: magnus.lucassen@awi.de.
authors.forename: Magnus
authors.initials: M
authors.lastname: Lucassen
mesh_terms: D000818:Animals; D021141:Nucleic Acid Amplification Techniques; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D012313:RNA
keywords: Antarctic Peninsula; Immune system; Non-model species; Nototheniidae; RNA-Seq