title: Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland.
abstract: BACKGROUND
  Dengue viruses are the most widespread arboviruses (transmitted mainly by Aedes aegypti and Ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. There are four dengue virus serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. Subsequent infections increase the risk of developing severe dengue fever.
  
  
  METHODS
  Samples from travellers returning from the endemic area of dengue fever were examined with the reverse transcription-polymerase chain reaction method. Primers amplified 743 bp fragment of the NS5 and 3'UTR genomic region of mosquito-borne flaviviruses of JEV group were used.
  
  
  RESULTS
  The sequences from the 10 examined patients were compared to available DENV sequences in GenBank database and the basic local alignment search tool (BLAST) results confirmed that the infective virus was DENV-1 (6 patients), DENV-2 (2 patients) and DENV-3 (2 patients).
  
  
  CONCLUSIONS
  For the first time in Poland, dengue virus serotypes were determined in travellers returning from dengue-endemic areas.
authors.affiliation: Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine, Medical University of Gdansk, Poland. bebe@gumed.edu.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Biernat
authors.forename: Joanna
authors.initials: J
authors.lastname: Stańczak
authors.forename: Beata
authors.initials: B
authors.lastname: Szostakowska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wroczyńska
authors.forename: Anna
authors.initials: A
authors.lastname: Kuna
authors.forename: Wacław L
authors.initials: WL
authors.lastname: Nahorski
authors.forename: Maria
authors.initials: M
authors.lastname: Racewicz
mesh_terms: D003715:Dengue / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics; D006801:Humans; D011044:Poland / Q000453:epidemiology; D012189:Retrospective Studies; D016415:Sequence Alignment; D065288:Serogroup; D014195:Travel