title: Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice.
abstract: Quercetin, a dietary flavonoid used as a food supplement, showed powerful antioxidant effects in different cellular models. However, recent in vitro and in vivo studies in mammals have suggested a prooxidant effect of quercetin and described an interaction with mitochondria causing an increase in O2 (∙-) production, a decrease in ATP levels, and impairment of respiratory chain in liver tissue. Therefore, because of its dual actions, we studied the effect of quercetin in vivo to analyze heart mitochondrial function and erythropoiesis. Mice were injected with 50 mg/kg of quercetin for 15 days. Treatment with quercetin decreased body weight, serum insulin, and ceruloplasmin levels as compared with untreated mice. Along with an impaired antioxidant capacity in plasma, quercetin-treated mice showed a significant delay on erythropoiesis progression. Heart mitochondrial function was also impaired displaying more protein oxidation and less activity for IV, respectively, than no-treated mice. In addition, a significant reduction in the protein expression levels of Mitofusin 2 and Voltage-Dependent Anion Carrier was observed. All these results suggest that quercetin affects erythropoiesis and mitochondrial function and then its potential use as a dietary supplement should be reexamined.
authors.affiliation: Center of Biomedical Research, Faculty of Health Sciences, Universidad Autónoma de Chile, Ricardo Morales 3369, 8910132 Santiago, Chile.
authors.forename: Lina M
authors.identifier: 0000-0002-9363-2730
authors.initials: LM
authors.lastname: Ruiz
authors.affiliation: Center of Biomedical Research, Faculty of Health Sciences, Universidad Autónoma de Chile, Ricardo Morales 3369, 8910132 Santiago, Chile.
authors.forename: Celia
authors.identifier: 0000-0002-8558-5444
authors.initials: C
authors.lastname: Salazar
authors.affiliation: Center for Biomedical Research, Faculty of Biological Sciences and Faculty of Medicine, Universidad Andres Bello, República 217, 8370146 Santiago, Chile.
authors.forename: Erik
authors.identifier: 0000-0002-2538-4093
authors.initials: E
authors.lastname: Jensen
authors.affiliation: iEng Solutions Ltd., London N12 0DR, UK.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Ruiz
authors.affiliation: Department of Chemical Sciences, Faculty of Exact Sciences, Universidad Andres Bello, República 275, 8370146 Santiago, Chile.
authors.forename: William
authors.identifier: 0000-0002-6061-8879
authors.initials: W
authors.lastname: Tiznado
authors.affiliation: Center of Biomedical Research, Faculty of Health Sciences, Universidad Autónoma de Chile, Ricardo Morales 3369, 8910132 Santiago, Chile.
authors.forename: Rodrigo A
authors.identifier: 0000-0002-9582-6379
authors.initials: RA
authors.lastname: Quintanilla
authors.affiliation: Center of Biomedical Research, Faculty of Health Sciences, Universidad Autónoma de Chile, Ricardo Morales 3369, 8910132 Santiago, Chile.
authors.forename: Marlen
authors.initials: M
authors.lastname: Barreto
authors.affiliation: Center for Biomedical Research, Faculty of Biological Sciences and Faculty of Medicine, Universidad Andres Bello, República 217, 8370146 Santiago, Chile ; Millennium Institute of Immunology and Immunotherapy, Santiago, Chile.
authors.forename: Alvaro A
authors.initials: AA
authors.lastname: Elorza
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D004920:Erythropoiesis / Q000187:drug effects*; D005293:Ferritins / Q000097:blood; D020558:GTP Phosphohydrolases / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D011794:Quercetin / Q000494:pharmacology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007328:Insulin; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; D005293:Ferritins; D011794:Quercetin; D002570:Ceruloplasmin; D020558:GTP Phosphohydrolases; C488751:Mfn2 protein, mouse