title: Magnetic fields suppress Pseudomonas aeruginosa biofilms and enhance ciprofloxacin activity.
abstract: Due to the refractory nature of pathogenic microbial biofilms, innovative biofilm eradication strategies are constantly being sought. Thus, this study addresses a novel approach to eradicate Pseudomonas aeruginosa biofilms. Magnetic nanoparticles (MNP), ciprofloxacin (Cipro), and magnetic fields were systematically evaluated in vitro for their relative anti-biofilm contributions. Twenty-four-hour biofilms exposed to aerosolized MNPs, Cipro, or a combination of both, were assessed in the presence or absence of magnetic fields (Static one-sided, Static switched, Oscillating, Static + oscillating) using changes in bacterial metabolism, biofilm biomass, and biofilm imaging. The biofilms exposed to magnetic fields alone exhibited significant metabolic and biomass reductions (p < 0.05). When biofilms were treated with a MNP/Cipro combination, the most significant metabolic and biomass reductions were observed when exposed to static switched magnetic fields (p < 0.05). The exposure of P. aeruginosa biofilms to a static switched magnetic field alone, or co-administration with MNP/Cipro/MNP + Cipro appears to be a promising approach to eradicate biofilms of this bacterium.
authors.affiliation: a College of Pharmacy , The University of Texas at Austin , Austin , TX , USA.
authors.forename: H M H N
authors.initials: HM
authors.lastname: Bandara
authors.forename: D
authors.initials: D
authors.lastname: Nguyen
authors.forename: S
authors.initials: S
authors.lastname: Mogarala
authors.forename: M
authors.initials: M
authors.lastname: Osiñski
authors.forename: H D C
authors.initials: HD
authors.lastname: Smyth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms; D002939:Ciprofloxacin / Q000494:pharmacology*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D058185:Magnetite Nanoparticles; D002939:Ciprofloxacin
keywords: Pseudomonas aeruginosa; biofilms; ciprofloxacin; drug delivery; magnetic fields; magnetic nanoparticles