title: Construction of a Near-Infrared-Activatable Enzyme Platform To Remotely Trigger Intracellular Signal Transduction Using an Upconversion Nanoparticle.
abstract: Photoactivatable (caged) bioeffectors provide a way to remotely trigger or disable biochemical pathways in living organisms at a desired time and location with a pulse of light (uncaging), but the phototoxicity of ultraviolet (UV) often limits its application. In this study, we have demonstrated the near-infrared (NIR) photoactivatable enzyme platform using protein kinase A (PKA), an important enzyme in cell biology. We successfully photoactivated PKA using NIR to phosphorylate its substrate, and this induced a downstream cellular response in living cells with high spatiotemporal resolution. In addition, this system allows NIR to selectively activate the caged enzyme immobilized on the nanoparticle surface without activating other caged proteins in the cytosol. This NIR-responsive enzyme-nanoparticle system provides an innovative approach to remote-control proteins and enzymes, which can be used by researchers who need to avoid direct UV irradiation or use UV as a secondary channel to turn on a bioeffector.
authors.affiliation: †Institute of Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
authors.forename: Hua-De
authors.initials: HD
authors.lastname: Gao
authors.affiliation: †Institute of Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
authors.forename: Pounraj
authors.initials: P
authors.lastname: Thanasekaran
authors.affiliation: †Institute of Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
authors.forename: Chao-Wei
authors.initials: CW
authors.lastname: Chiang
authors.affiliation: †Institute of Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Hong
authors.affiliation: ‡Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, 1 Zhongxiao E. Road, Section 3, Taipei 10608, Taiwan.
authors.forename: Yen-Chun
authors.initials: YC
authors.lastname: Liu
authors.affiliation: ‡Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, 1 Zhongxiao E. Road, Section 3, Taipei 10608, Taiwan.
authors.forename: Yu-Hsu
authors.initials: YH
authors.lastname: Chang
authors.affiliation: †Institute of Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
authors.forename: Hsien-Ming
authors.initials: HM
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism / Q000528:radiation effects*; D004800:Enzymes, Immobilized / Q000378:metabolism / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism; D007259:Infrared Rays; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction
chemical_list: D004800:Enzymes, Immobilized; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: cellular activity; enzyme; photoactivation; proteins; upconversion nanoparticles