title: Interaction of γ-Fe₂O₃ nanoparticles with fibrinogen.
abstract: In this article, an attempt is made to analysis the binding mechanism of γ-Fe2O3 nanoparticles with fibrinogen by using a combination of circular dichroism, UV-vis, fluorescence spectroscopic and computational methods. The multi-spectroscopic data revealed that the complex easily formed between γ-Fe2O3 nanoparticles and fibrinogen by mainly hydrogen bonding forces. The binding constants of fibrinogen with γ-Fe2O3 nanoparticles were 2.24×10(7), 1.15×10(7) and 0.72×10(7)Lmol(-1) at 298, 304, and 310K, respectively. Furthermore, the results from circular dichroism, UV-vis, synchronous fluorescence, and three-dimensional fluorescence studies showed that the strong binding interaction of γ-Fe2O3 nanoparticles with fibrinogen induced an obvious perturbation in the protein secondary and tertiary structure. Moreover, the results of molecular modeling indicated the existence of the preferable binding site on fibrinogen for γ-Fe2O3 NPs model.
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Peirong
authors.initials: P
authors.lastname: Wu
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: wyqing76@126.com.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006860:Hydrogen Bonding; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
chemical_list: D005290:Ferric Compounds; D005340:Fibrinogen
keywords: Conformational changes; Fibrinogen; Fluorescence; Protein interaction; γ-Fe(2)O(3) nanoparticles