title: Effect of magnesium supplementation on blood pressure and vascular reactivity in nitric oxide synthase inhibition-induced hypertension model.
abstract: The aim of this study was to assess the effect of oral magnesium supplementation (Mg-supp) on blood pressure (BP) and possible mechanism in nitric oxide synthase (NOS) inhibition-induced hypertension model. Hypertension and/or Mg-supp were created by N-nitro-l-arginine methyl ester (25 mg/kg/day by drinking water) and magnesium-oxide (0.8% by diet) for 6 weeks. Systolic BP was measured weekly by tail-cuff method. The effects of hypertension and/or Mg-supp in thoracic aorta and third branch of mesenteric artery constriction and relaxation responses were evaluated. NOS-inhibition produced a gradually developing hypertension and the magnitude of the BP was significantly attenuated after five weeks of Mg-supp. The increased phenylephrine-induced contractile and decreased acetylcholine (ACh)-induced dilation responses were found in both artery segments of hypertensive groups. Mg-supp was restored ACh-relaxation response in both arterial segments and also Phe-constriction response in thoracic aorta but not in mesenteric arteries. The contributions of NO, prostaglandins and K(+) channels to the dilator response of ACh were similar in the aorta of all the groups. The contribution of the NO to the ACh-mediated relaxation response of mesenteric arteries was suppressed in hypertensive rats, whereas this was corrected by Mg-supp. The flow-mediated dilation response of mesenteric arteries in hypertensive rats failed and could not be corrected by Mg-supp. Whereas, vascular eNOS protein and magnesium levels were not changed and plasma nitrite levels were reduced in hypertensive rats. The results of this study showed that Mg-supp lowered the arterial BP in NOS-inhibition induced hypertension model by restoring the agonist-induced relaxation response of the arteries.
authors.affiliation: a Department of Physiology, Medical Faculty , Akdeniz University , Antalya , Turkey .
authors.forename: Filiz
authors.initials: F
authors.lastname: Basralı
authors.affiliation: b Department of Physiology, Medical Faculty , Near East University , Nicosia , Mersin , Turkey .
authors.forename: Günnur
authors.initials: G
authors.lastname: Koçer
authors.affiliation: a Department of Physiology, Medical Faculty , Akdeniz University , Antalya , Turkey .
authors.forename: Pınar
authors.initials: P
authors.lastname: Ülker Karadamar
authors.affiliation: a Department of Physiology, Medical Faculty , Akdeniz University , Antalya , Turkey .
authors.forename: Seher
authors.initials: S
authors.lastname: Nasırcılar Ülker
authors.affiliation: c Department of Histology and Embryology, Medical Faculty , Akdeniz University , Antalya , Turkey , and.
authors.forename: Leyla
authors.initials: L
authors.lastname: Satı
authors.affiliation: a Department of Physiology, Medical Faculty , Akdeniz University , Antalya , Turkey .
authors.forename: Nur
authors.initials: N
authors.lastname: Özen
authors.affiliation: d Department of Chemistry, Faculty of Sciences and Letters , Istanbul Technical University , Istanbul , Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Özyurt
authors.affiliation: a Department of Physiology, Medical Faculty , Akdeniz University , Antalya , Turkey .
authors.forename: Ümit Kemal
authors.initials: ÜK
authors.lastname: Şentürk
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006973:Hypertension / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D008274:Magnesium / Q000008:administration & dosage*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D019001:Nitric Oxide Synthase; D008274:Magnesium; D019331:NG-Nitroarginine Methyl Ester
keywords: Hypertension; blood pressure; magnesium; nitric oxide; vascular tonus