title: Cellulose production in Pseudomonas syringae pv. syringae: a compromise between epiphytic and pathogenic lifestyles.
abstract: Genome sequencing and annotation have revealed a putative cellulose biosynthetic operon in the strain Pseudomonas syringae pv. syringae UMAF0158, the causal agent of bacterial apical necrosis. Bioinformatics analyses and experimental methods were used to confirm the functionality of the cellulose biosynthetic operon. In addition, the results showed the contribution of the cellulose operon to important aspects of P. syringae pv. syringae biology, such as the formation of biofilms and adhesion to the leaf surface of mango, suggesting that this operon increases epiphytic fitness. However, based on the incidence and severity of the symptoms observed in tomato leaflets, cellulose expression reduces virulence, as cellulose-deficient mutants increased the area of necrosis, whereas the cellulose-overproducing strain decreased the area of necrosis compared with the wild type. In conclusion, the results of this study show that the epiphytic and pathogenic stages of the P. syringae pv. syringae UMAF0158 lifestyle are intimately affected by cellulose production.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Estación Experimental La Mayora, Algarrobo-Costa, 29750 Málaga, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Arrebola
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Facultad de Ciencias, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Víctor J
authors.initials: VJ
authors.lastname: Carrión
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Facultad de Ciencias, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Gutiérrez-Barranquero
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Facultad de Ciencias, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Pérez-García
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Parque Científico y Tecnológico de la Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Rodríguez-Palenzuela
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Facultad de Ciencias, Campus de Teatinos, 29071 Málaga, Spain.
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Cazorla
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Facultad de Ciencias, Campus de Teatinos, 29071 Málaga, Spain adevicente@uma.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: de Vicente
mesh_terms: D001483:Base Sequence; D018441:Biofilms; D002482:Cellulose / Q000096:biosynthesis* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000382:microbiology; D008969:Molecular Sequence Data; D009876:Operon; D018515:Plant Leaves / Q000382:microbiology; D044224:Pseudomonas syringae / Q000235:genetics* / Q000378:metabolism*; D014774:Virulence / Q000235:genetics
chemical_list: D002482:Cellulose
keywords: bacterial EPS; biofilm; phyllosphere; virulence; woody plants