title: Calebin-A inhibits adipogenesis and hepatic steatosis in high-fat diet-induced obesity via activation of AMPK signaling.
abstract: METHODS
  Diet-induced obesity and associated nonalcoholic fatty liver disease have increased and become a major health problem worldwide. This study was conducted to investigate the chemopreventive effects of dietary Calebin-A, a curcuminoid, on differentiation of 3T3-L1 adipocytes and high-fat diet (HFD) induced obesity and hepatic steatosis. Potential mechanisms contributing to these effects were also elucidated.
  
  
  RESULTS
  Calebin-A effectively and dose dependently suppressed accumulation of lipid droplets in adipocytes through the suppression of adipogenic specific factor peroxisome proliferator-activated receptor (PPAR) γ and fatty acid synthase and activated acetyl-CoA carboxylase. Dietary Calebin-A effectively decreased weight gain and relative perigonadal, retroperitoneal, and mesenteric fat weight in HFD-fed mice. Furthermore, Calebin-A markedly reduced hepatic steatosis and the serum levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, total cholesterol, and triacylglycerol. These effects were associated with the downregulation of PPARγ, sterol regulatory element-binding protein-1, and particularly the activation of AMP-activated protein kinase α signaling found in both adipocytes and liver tissues.
  
  
  CONCLUSIONS
  Taken together, these results demonstrated for the first time that Calebin-A suppressed adipocyte differentiation, prevented HFD-induced obesity, and improved hepatic steatosis, suggesting a novel application for the prevention and treatment of obesity and associated nonalcoholic fatty liver disease.
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Ching-Shu
authors.initials: CS
authors.lastname: Lai
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Sih-Ning
authors.initials: SN
authors.lastname: Liao
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Tsai
authors.affiliation: Sabinsa Corporation, East Windsor, NJ, USA.
authors.forename: Nagabhushanam
authors.initials: N
authors.lastname: Kalyanam
authors.affiliation: Sabinsa Corporation, East Windsor, NJ, USA.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Majeed
authors.affiliation: Research and Development, Sami Labs Limited, Bangalore, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Majeed
authors.affiliation: Department of Food Science, Rutgers University, New Brunswick, NJ, USA.
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Min-Hsiung
authors.initials: MH
authors.lastname: Pan
mesh_terms: D041721:3T3-L1 Cells / Q000187:drug effects; D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D059305:Diet, High-Fat / Q000009:adverse effects; D005234:Fatty Liver / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D039821:Monoterpenes / Q000494:pharmacology*; D009765:Obesity / Q000209:etiology / Q000517:prevention & control*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002934:Cinnamates; D039821:Monoterpenes; C448819:calebin-A; D055372:AMP-Activated Protein Kinases
keywords: 3T3-L1 adipocytes; AMP-activated protein kinase; Adipogenesis; Calebin-A; High-fat diet