title: Prostaglandin E2 increases proximal tubule fluid reabsorption, and modulates cultured proximal tubule cell responses via EP1 and EP4 receptors.
abstract: Renal prostaglandin (PG) E2 regulates salt and water transport, and affects disease processes via EP1-4 receptors, but its role in the proximal tubule (PT) is unknown. Our study investigates the effects of PGE2 on mouse PT fluid reabsorption, and its role in growth, sodium transporter expression, fibrosis, and oxidative stress in a mouse PT cell line (MCT). To determine which PGE2 EP receptors are expressed in MCT, qPCR for EP1-4 was performed on cells stimulated for 24 h with PGE2 or transforming growth factor beta (TGFβ), a known mediator of PT injury in kidney disease. EP1 and EP4 were detected in MCT, but EP2 and EP3 are not expressed. EP1 was increased by PGE2 and TGFβ, but EP4 was unchanged. To confirm the involvement of EP1 and EP4, sulprostone (SLP, EP1/3 agonist), ONO8711 (EP1 antagonist), and EP1 and EP4 siRNA were used. We first show that PGE2, SLP, and TGFβ reduced H(3)-thymidine and H(3)-leucine incorporation. The effects on cell-cycle regulators were examined by western blot. PGE2 increased p27 via EP1 and EP4, but TGFβ increased p21; PGE2-induced p27 was attenuated by TGFβ. PGE2 and SLP reduced cyclinE, while TGFβ increased cyclinD1, an effect attenuated by PGE2 administration. Na-K-ATPase α1 (NaK) was increased by PGE2 via EP1 and EP4. TGFβ had no effect on NaK. Additionally, PGE2 and TGFβ increased fibronectin levels, reaching 12-fold upon co-stimulation. EP1 siRNA abrogated PGE2-fibronectin. PGE2 also increased ROS generation, and ONO-8711 blocked PGE2-ROS. Finally, PGE2 significantly increased fluid reabsorption by 31 and 46% in isolated perfused mouse PT from C57BL/6 and FVB mice, respectively, and this was attenuated in FVB-EP1 null mice. Altogether PGE2 acting on EP1 and EP4 receptors may prove to be important mediators of PT injury, and salt and water transport.
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Rania
authors.initials: R
authors.lastname: Nasrallah
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Ramzi
authors.initials: R
authors.lastname: Hassouneh
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Joseph
authors.initials: J
authors.lastname: Zimpelmann
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Karam
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.|Kidney Research Centre, The Ottawa Hospital Research Institute, Ottawa, ON, Canada.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Thibodeau
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.|Kidney Research Centre, The Ottawa Hospital Research Institute, Ottawa, ON, Canada.
authors.forename: Dylan
authors.initials: D
authors.lastname: Burger
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.|Kidney Research Centre, The Ottawa Hospital Research Institute, Ottawa, ON, Canada.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Burns
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.|Kidney Research Centre, The Ottawa Hospital Research Institute, Ottawa, ON, Canada.
authors.forename: Chris Rj
authors.initials: CR
authors.lastname: Kennedy
authors.affiliation: Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hébert
mesh_terms: D000166:Acridines; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D002208:Caproates / Q000494:pharmacology; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D015232:Dinoprostone / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D034741:RNA, Small Interfering / Q000494:pharmacology; D058306:Receptors, Prostaglandin E, EP1 Subtype / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D058309:Receptors, Prostaglandin E, EP4 Subtype / Q000378:metabolism*; D065608:Renal Reabsorption / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D000166:Acridines; D001643:Bridged Bicyclo Compounds; D002208:Caproates; D019927:Cyclin E; C122148:ONO 8711; C545636:Ptger1 protein, mouse; C545651:Ptger4 protein, mouse; D034741:RNA, Small Interfering; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D016212:Transforming Growth Factor beta; D019938:Cyclin D1; C033472:10,10'-dimethyl-9,9'-biacridinium; C016767:sulprostone; D015232:Dinoprostone