title: PDIA3 and PDIA6 gene expression as an aggressiveness marker in primary ductal breast cancer.
abstract: Changes in the expression of the protein disulfide isomerase genes PDIA3 and PDIA6 may increase endoplasmic reticulum stress, leading to cellular instability and neoplasia. We evaluated the expression of PDIA3 and PDIA6 in invasive ductal carcinomas. Using reverse transcription-quantitative polymerase chain reaction, we compared the mRNA expression level in 45 samples of invasive ductal carcinoma with that in normal breast samples. Increased expression of the PDIA3 gene in carcinomas (P = 0.0009) was observed. In addition, PDIA3 expression was increased in tumors with lymph node metastasis (P = 0.009) and with grade III (P < 0.02). The PDIA6 gene showed higher expression levels in the presence of lymph node metastasis (U = 99.00, P = 0.0476) and lower expression for negative hormone receptors status (P = 0.0351). Our results suggest that alterations in PDIA3/6 expression levels may be involved in the breast carcinogenic process and should be further investigated as a marker of aggressiveness.
authors.affiliation: Laboratório de Citogenética Humana e Oncogenética, Departamento de Genética, Universidade Federal do Paraná.
authors.forename: F S
authors.initials: FS
authors.lastname: Ramos
authors.affiliation: Laboratório de Citogenética Humana e Oncogenética, Departamento de Genética, Universidade Federal do Paraná.
authors.forename: L T R
authors.initials: LT
authors.lastname: Serino
authors.affiliation: Laboratório de Citogenética Humana e Oncogenética, Departamento de Genética, Universidade Federal do Paraná.
authors.forename: C M S
authors.initials: CM
authors.lastname: Carvalho
authors.affiliation: Universidade de Mama, Hospital Nossa Senhora das Graças, Curitiba, PR, Brasil.
authors.forename: R S
authors.initials: RS
authors.lastname: Lima
authors.affiliation: Universidade de Mama, Hospital Nossa Senhora das Graças, Curitiba, PR, Brasil.
authors.forename: C A
authors.initials: CA
authors.lastname: Urban
authors.affiliation: Laboratório de Citogenética Humana e Oncogenética, Departamento de Genética, Universidade Federal do Paraná.
authors.forename: I J
authors.initials: IJ
authors.lastname: Cavalli
authors.affiliation: Laboratório de Citogenética Humana e Oncogenética, Departamento de Genética, Universidade Federal do Paraná, eribeiro@ufpr.br.
authors.forename: E M S F
authors.initials: EM
authors.lastname: Ribeiro
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D019704:Protein Disulfide-Isomerases / Q000235:genetics* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C496931:PDIA6 protein, human; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human