title: Old is new again: a chemical probe for targeting mitochondria and monitoring mitochondrial membrane potential in cells.
abstract: Here, we explore the new application of an old molecule. We find that the tetraphenylethene-indolium molecule (TPE-indo) can both image the mitochondria (in the aggregated state), and indicate mitochondrial activity by the fluorescence change of TPE-indo. TPE-indo shows good photostability, longer emission wavelength, targeting effect for mitochondria, and better response to the changes of the mitochondrial membrane potential (ΔΨm).
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No. 11 ZhongGuanCun BeiYiTiao, 100190 Beijing, China. xingyujiang@nanoctr.cn wangz@nanoctr.cn.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.forename: Xianhong
authors.initials: X
authors.lastname: Huang
authors.forename: Guanxin
authors.initials: G
authors.lastname: Zhang
authors.forename: Xuefei
authors.initials: X
authors.lastname: Wang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D015335:Molecular Probes / Q000737:chemistry*; D041681:NIH 3T3 Cells
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D015335:Molecular Probes