title: Influenza bivalent vaccine comprising recombinant H3 hemagglutinin (HA) and H1 HA containing replaced H3 hemagglutinin transmembrane domain exhibited improved heterosubtypic protection immunity in mice.
abstract: Influenza caused by infection of influenza viruses is still a leading cause of morbidity and mortality in human. Vaccination is the main defense against influenza virus, but current influenza trivalent or quatrivalent vaccines (TIV/QIV) would lose their effectiveness when vaccine strains are mismatched with circulating strains. Our early study showed that recombinant influenza Hx-TM HA proteins containing H3 HA transmembrane domain(TM) had improved immunogenicity and heterosubtypic protection over corresponding wild-type Hx-WT HA proteins. In present study, bivalent vaccines containing H3-WT+Hx-TM were investigated for their immune responses and heterosubtypic protection immunities. The data showed that the bivalent vaccines containing H3-WT and H5-TM or H1-TM had improved immune responses and heterosubtypic protection over the bivalent vaccines containing H3-WT and H5-WT or H1-WT respectively. These results demonstrated that the improved immune responses and heterosubtypic protection of Hx-TM HA proteins could be translated into bivalent vaccines, suggesting a feasible strategy of improving the immune responses and heterosubtypic protection of influenza multivalent vaccines such as TIV and QIV.
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China; Department of biotechnology, Guilin Medical University, Guilin 541000, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Chunyi
authors.initials: C
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Firstline Biopharmaceuticals Corporation, 12050 167th PL NE, Redmond, WA 98052, USA.
authors.forename: George Dacai
authors.initials: GD
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China. Electronic address: caoych@mail.sysu.edu.cn.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001835:Body Weight; D056738:Cross Protection; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D009975:Orthomyxoviridae / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic
keywords: Heterosubtypic protection; Influenza A virus; Multivalent vaccine