title: Applying a kinetic method to an indirect ELISA measuring Ostertagia ostertagi antibodies in milk.
abstract: Indirect enzyme-linked immunosorbent assays (ELISAs) are frequently run as endpoint ELISAs (e-ELISAs). However, kinetic ELISAs (k-ELISAs) have certain advantages over e-ELISAs. The objective of this study was to understand the relationship between e-ELISA and k-ELISA results. Specifically, to determine whether it was possible to run both k-ELISA and e-ELISA on the same plate and establish an appropriate time interval for k-ELISA measurements. A normalization method for k-ELISA slopes (slope ratio) is proposed. Using an indirect e-ELISA test measuring antibodies against Ostertagia ostertagi in milk from dairy cattle, we found that running a k-ELISA had no effect on optical density ratio results of an e-ELISA on the same plate, and that agreement was very strong at 10, 15, and 28 min, allowing for a reduction in the total processing time for ELISA tests.
authors.affiliation: Department of Health Management (Vanderstichel, Dohoo); Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island (Markham).
authors.forename: Raphaël
authors.initials: R
authors.lastname: Vanderstichel
authors.affiliation: Department of Health Management (Vanderstichel, Dohoo); Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island (Markham).
authors.forename: Ian
authors.initials: I
authors.lastname: Dohoo
authors.affiliation: Department of Health Management (Vanderstichel, Dohoo); Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island (Markham).
authors.forename: Fred
authors.initials: F
authors.lastname: Markham
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008892:Milk / Q000737:chemistry*; D010028:Ostertagia / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth