title: The antioxidant effect of dexmedetomidine on testicular ischemia-reperfusion injury.
abstract: OBJECTIVE
  To investigate the protective effect of dexmedetomidine (Dex) on testicular damage induced by ischemia-reperfusion injury in rats.
  
  
  METHODS
  Sham group underwent left scrotal exploration only (group 1). The ischemia-reperfusion only group underwent left testicular torsion and detorsion (group 2). The ischemia-reperfusion plus Dex group underwent left testicular torsion, received 50 µg/kg Dex (group 3) and 100 µg/kg Dex (group 4) intraperitoneally at minute 180 of ischemia and then underwent detorsion. We determined histopathological findings and performed specific biochemical analyses.
  
  
  RESULTS
  Increasing doses of Dex significantly increased TAS, and significantly decreased OSI. Analyzing the antioxidant effects of increasing doses of Dex in torsion and contrlateral testicles: Dex 100 µg/kg statistically significant increased the tissue total antioxidant status (TAS) and oxidative stress index (OSI) when compared with Dex 50 µg/kg but not found significantly change on the tissue total oxidant status (TOS). However, Dex did not significantly improve these histological alterations.
  
  
  CONCLUSIONS
  The antioxidant effects of dexmedetomidine on testicular ischemia-reperfusion injury in ipsilateral and contrlateral testis, but in the histopathological level, there was no difference statistically according to Johnsen's scoring system between groups at both sides.
authors.affiliation: Department of Urology, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Devrim
authors.initials: D
authors.lastname: Tuglu
authors.affiliation: Department of Urology, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Ercan
authors.initials: E
authors.lastname: Yuvanc
authors.affiliation: Department of Urology, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Erdal
authors.initials: E
authors.lastname: Yilmaz
authors.affiliation: Department of Anesthesiology and Reanimation, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Isin Yazici
authors.initials: IY
authors.lastname: Gencay
authors.affiliation: Department of Pathology, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Atasoy
authors.affiliation: Department of Biochemistry, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Ucler
authors.initials: U
authors.lastname: Kisa
authors.affiliation: Department of Urology, Medical Faculty, University of Kirikkale, Kirikkale, Turkey.
authors.forename: Ertan
authors.initials: E
authors.lastname: Batislam
mesh_terms: D058647:Adrenergic alpha-2 Receptor Agonists / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D020927:Dexmedetomidine / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D007511:Ischemia / Q000473:pathology / Q000517:prevention & control; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011446:Prospective Studies; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D012720:Severity of Illness Index; D013086:Spermatic Cord Torsion / Q000473:pathology / Q000517:prevention & control*; D013737:Testis / Q000098:blood supply* / Q000473:pathology; D013997:Time Factors
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D000975:Antioxidants; D020927:Dexmedetomidine