title: Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and Nodule Sinorhizobium with Medicago sativa L.
abstract: Seed inoculation with bacterial consortium was found to increase legume yield, providing a higher growth than the standard nitrogen treatment methods. Alfalfa plants were inoculated by mono- and binary compositions of nitrogen-fixing microorganisms. Their physiological and biochemical properties were estimated. Inoculation by microbial consortium of Sinorhizobium meliloti T17 together with a new cyanobacterial isolate Nostoc PTV was more efficient than the single-rhizobium strain inoculation. This treatment provides an intensification of the processes of biological nitrogen fixation by rhizobia bacteria in the root nodules and an intensification of plant photosynthesis. Inoculation by bacterial consortium stimulates growth of plant mass and rhizogenesis and leads to increased productivity of alfalfa and to improving the amino acid composition of plant leaves. The full nucleotide sequence of the rRNA gene cluster and partial sequence of the dinitrogenase reductase (nifH) gene of Nostoc PTV were deposited to GenBank (JQ259185.1, JQ259186.1). Comparison of these gene sequences of Nostoc PTV with all sequences present at the GenBank shows that this cyanobacterial strain does not have 100% identity with any organisms investigated previously. Phylogenetic analysis showed that this cyanobacterium clustered with high credibility values with Nostoc muscorum.
authors.affiliation: Educational and Scientific "Institute of Biology", Taras Shevchenko National University of Kyiv, 64/13 Volodymyrska Street, Kyiv 01601, Ukraine.
authors.forename: E V
authors.initials: EV
authors.lastname: Karaushu
authors.affiliation: N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119333, Russia.
authors.forename: I V
authors.identifier: 0000-0001-7548-3615
authors.initials: IV
authors.lastname: Lazebnaya
authors.affiliation: Lomonosov Moscow State University, Biocenter, Leninskie Gory 1-12, Moscow 119991, Russia.
authors.forename: T R
authors.initials: TR
authors.lastname: Kravzova
authors.affiliation: Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, 31/17 Vasylkivska Street, Kyiv 03022, Ukraine.
authors.forename: N A
authors.initials: NA
authors.lastname: Vorobey
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova Street 26, Moscow 119334, Russia.
authors.forename: O E
authors.identifier: 0000-0003-3136-0415
authors.initials: OE
authors.lastname: Lazebny
authors.affiliation: Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, 31/17 Vasylkivska Street, Kyiv 03022, Ukraine.
authors.forename: D A
authors.initials: DA
authors.lastname: Kiriziy
authors.affiliation: Educational and Scientific "Institute of Biology", Taras Shevchenko National University of Kyiv, 64/13 Volodymyrska Street, Kyiv 01601, Ukraine.
authors.forename: O P
authors.initials: OP
authors.lastname: Olkhovich
authors.affiliation: Educational and Scientific "Institute of Biology", Taras Shevchenko National University of Kyiv, 64/13 Volodymyrska Street, Kyiv 01601, Ukraine.
authors.forename: N Yu
authors.initials: NY
authors.lastname: Taran
authors.affiliation: Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, 31/17 Vasylkivska Street, Kyiv 03022, Ukraine.
authors.forename: S Ya
authors.initials: SY
authors.lastname: Kots
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia.
authors.forename: A A
authors.identifier: 0000-0001-5958-7350
authors.initials: AA
authors.lastname: Popova
authors.affiliation: Lomonosov Moscow State University, Belozersky Institute of Physical-Chemical Biology, Leninskie Gory 1-40, Moscow 119992, Russia.
authors.forename: E
authors.initials: E
authors.lastname: Omarova
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia ; Lomonosov Moscow State University, Belozersky Institute of Physical-Chemical Biology, Leninskie Gory 1-40, Moscow 119992, Russia.
authors.forename: O A
authors.identifier: 0000-0003-2913-9017
authors.initials: OA
authors.lastname: Koksharova
mesh_terms: D000383:Agriculture; D000458:Cyanobacteria / Q000378:metabolism / Q000502:physiology*; D000455:Medicago sativa / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation / Q000502:physiology*; D010802:Phylogeny; D012231:Rhizobium / Q000378:metabolism / Q000502:physiology; D053204:Root Nodules, Plant / Q000382:microbiology* / Q000502:physiology; D016962:Sinorhizobium meliloti / Q000378:metabolism / Q000502:physiology; D013559:Symbiosis
chemical_list: D009584:Nitrogen