title: Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract.
abstract: Bauhinia forficata Link. is utilised as an antidiabetic in Brazilian folk-medicine; furthermore, its antioxidant properties suggest a potential usefulness in the prevention of diabetes complications associated with oxidative stress. The contribution of a flavonoid-rich fraction (FRF), HPLC-PDA-ESI-MS characterised, to the antioxidant and cytotoxic properties of B. forficata hydro-alcoholic leaves extract was evaluated for the first time. Both extract and FRF showed radical-scavenging activity and reducing power with a strong relationship with the flavonoid content found; hence, flavonoids are mainly responsible for the primary antioxidant activity of B. forficata extract. The extract significantly decreased FO-1 cell viability at the higher concentrations. FRF did not exert any effect; thus, flavonoids do not appear to be responsible for the cytotoxicity of the extract. The extract resulted virtually non-toxic against both Artemia salina and normal human lymphocytes, demonstrating potential selectivity in inhibiting cancer cell growth. Finally, no antimicrobial activity was observed against the bacteria and yeasts tested.
authors.affiliation: a 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.
authors.forename: Natalizia
authors.initials: N
authors.lastname: Miceli
authors.affiliation: a 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.
authors.forename: Luigina Pasqualina
authors.initials: LP
authors.lastname: Buongiorno
authors.affiliation: a 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Celi
authors.affiliation: b 'Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute' Department , University of Messina , Viale F. Stagno d'Alcontres 31, Messina 98166 , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.affiliation: a 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.|c Chromaleont s.r.l., c/o 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.|d Centro Integrato di Ricerca, University Campus Bio-Medico of Rome , Via Álvaro del Portillo 21, Roma 00128 , Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.affiliation: b 'Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute' Department , University of Messina , Viale F. Stagno d'Alcontres 31, Messina 98166 , Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Donato
authors.affiliation: a 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.|c Chromaleont s.r.l., c/o 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.|d Centro Integrato di Ricerca, University Campus Bio-Medico of Rome , Via Álvaro del Portillo 21, Roma 00128 , Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
authors.affiliation: e 'Patologia Umana' Department , University of Messina , A.O.U. Policlinico 'G.Martino' Via Consolare Valeria 1, Messina 98124 , Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Bonaccorsi
authors.affiliation: a 'Scienze del Farmaco e Prodotti per la Salute' Department , University of Messina , Viale Annunziata, Messina 98168 , Italy.
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Taviano
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001156:Artemia / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D036481:Bauhinia / Q000737:chemistry*; D001938:Brazil; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004354:Drug Screening Assays, Antitumor; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000633:toxicity; D015003:Yeasts / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts
keywords: Bauhinia forficata Link.; antioxidant activity; cytotoxic activity; flavonoid-rich fraction; phenolic profile