title: Cytotoxic and genotoxic effects of abamectin, chlorfenapyr, and imidacloprid on CHOK1 cells.
abstract: The cytotoxicity and genotoxicity of abamectin, chlorfenapyr, and imidacloprid have been evaluated on the Chinese hamster ovary (CHOK1) cells. Neutral red incorporation (NRI), total cellular protein content (TCP), and methyl tetrazolium (MTT) assays were followed to estimate the mid-point cytotoxicity values, NRI50, TCP50, and MTT50, respectively. The effects of the sublethal concentration (NRI25) on glutathione S-transferase (GST), glutathione reductase (GRD), glutathione peroxidase (GPX), and total glutathione content have been evaluated in the presence and absence of reduced glutathione (GSH), vitamin C, and vitamin E. The genotoxicity was evaluated using chromosomal aberrations (CA), micronucleus (MN) formation, and DNA fragmentation techniques in the presence and absence of the metabolic activation system, S9 mix. Abamectin was the most cytotoxic pesticide followed by chlorfenapyr, while imidacloprid was the least cytotoxic one. The glutathione redox cycle components were altered by the tested pesticides in the absence and presence of the tested antioxidants. The results of genotoxicity indicate that abamectin, chlorfenapyr, and imidacloprid have potential genotoxic effects on CHOK1 cells under the experimental conditions.
authors.affiliation: Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
authors.forename: Ali S
authors.initials: AS
authors.lastname: Al-Sarar
authors.affiliation: Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia. ymohamed@ksu.edu.sa.
authors.forename: Yasser
authors.initials: Y
authors.lastname: Abobakr
authors.affiliation: Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
authors.forename: Alaa E
authors.initials: AE
authors.lastname: Bayoumi
authors.affiliation: Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
authors.forename: Hamdy I
authors.initials: HI
authors.lastname: Hussein
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D053938:DNA Fragmentation / Q000187:drug effects*; D007093:Imidazoles / Q000633:toxicity*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*
chemical_list: D007093:Imidazoles; D009153:Mutagens; D000073943:Neonicotinoids; D009574:Nitro Compounds; D011722:Pyrethrins; C082359:imidacloprid; C048324:abamectin; D007559:Ivermectin; C436643:chlorfenapyr
keywords: Abamectin; CHOK1 cells; Chlorfenapyr; Cytotoxicity; Genotoxicity; Glutathione redox cycle; Imidacloprid