title: Phosphorus dendrimers and photodynamic therapy. Spectroscopic studies on two dendrimer-photosensitizer complexes: Cationic phosphorus dendrimer with rose bengal and anionic phosphorus dendrimer with methylene blue.
abstract: Dendrimers due to their unique architecture may play an important role in drug delivery systems including chemotherapy, gene therapy and recently, photodynamic therapy as well. We investigated two dendrimer-photosensitizer systems in context of potential use of these systems in photodynamic therapy. The mixtures of an anionic phosphorus dendrimer of the second generation and methylene blue were studied by UV-vis spectroscopy while that of a cationic phosphorus dendrimer (third generation) and rose bengal were investigated by spectrofluorimetric methods. Spectroscopic analysis of these two systems revealed the formation of dendrimer-photosensitizer complexes via electrostatic interactions as well as π stacking. The stoichiometry of the rose bengal-cationic dendrimer complex was estimated to be 7:1 and 9:1 for the methylene blue-anionic dendrimer complex. The results suggest that these polyanionic or polycationic phosphorus dendrimers can be promising candidates as carriers in photodynamic therapy.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Dabrzalska
authors.affiliation: Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Zablocka
authors.affiliation: Université Paris Descartes, PRES Sorbonne Paris Cité, CNRS UMR 860, Laboratoire de Chimie et de Biochimie pharmacologiques et toxicologique, 45 rue des Saints Pères, 75006 Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mignani
authors.affiliation: Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077 Toulouse, France; Université de Toulouse, UPS, INPT, 31077, Toulouse Cedex 4, France. Electronic address: jean-pierre.majoral@lcc-toulouse.fr.
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Majoral
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, 01069 Dresden, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert-Maculewicz
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D008751:Methylene Blue / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry*; D012395:Rose Bengal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D050091:Dendrimers; D017319:Photosensitizing Agents; D012395:Rose Bengal; D010758:Phosphorus; D008751:Methylene Blue
keywords: Drug delivery system; Methylene blue; Phosphorus dendrimer; Photodynamic therapy; Photosensitizer; Rose bengal