title: miR-135a Targets and Regulates Prolactin Receptor Gene in Goat Mammary Epithelial Cells.
abstract: Mammary gland development and lactation are typical traits controlled by multiple genes, hormones, and regulatory factors. Prolactin receptor (PRLR), a specific receptor of prolactin, has been reported to have important physiological functions in regulating mammogenesis and lactogenesis. However, the post-transcriptional regulation mechanisms of PRLR expression have not yet been shown in detail. In this study, the expression of miR-135a and PRLR at different development stages of Laoshan dairy goat mammary gland tissues was investigated. After overexpression and silencing expression of miR-135a in cultured primary mammary epithelial cells, the regulatory relationship between miR-135a and PRLR was examined through dual-luciferase reporter assay, and the expression of PRLR at both mRNA and protein levels was examined by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. Collectively, our results suggested that PRLR is a direct target gene of miR-135a, miR-135a is a novel regulator of PRLR, and it might play an essential role in the regulation of animal mammary gland development and lactation.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Guizhi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Tianle
authors.initials: T
authors.lastname: Chao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, China .
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003612:Dairying; D004847:Epithelial Cells / Q000378:metabolism* / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006041:Goats / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D007774:Lactation / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D035683:MicroRNAs / Q000502:physiology*; D008894:Milk Proteins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D011981:Receptors, Prolactin / Q000235:genetics* / Q000378:metabolism; D017385:Sequence Homology
chemical_list: D035683:MicroRNAs; D008894:Milk Proteins; D011981:Receptors, Prolactin