title: Interferon-γ and Interleukin-10 Gene Polymorphisms are not Predictors of Chronic Hepatitis C (Genotype-4) Disease Progression.
abstract: Immunoregulatory cytokines have an influence on hepatitis C virus (HCV) infection outcome. This study aimed to determine whether single nucleotide polymorphisms (SNP) in IFN- γ and IL-10 genes are associated with susceptibility and/or are markers of prognosis regarding chronic hepatitis C outcomes. IFN γ (+874T/A) and IL-10 (-1082G/A) genotypes were determined in 75 HCV genotype 4 patients with different disease severities (chronic hepatitis, n=25, liver cirrhosis and hepatocellular carcinoma (HCC) on top of liver cirrhosis, n=50) and 25 healthy participants using allele-specific polymerase chain reaction. No statistical differences in allele or genotype distributions of IFN γ and IL-10 genes were detected between patients and controls or between patientgroups. No significant difference in the frequency of IL-10 SNP at position -1082 or IFN-γ at position +874T/A was found between chronic HCV genotype 4 and with progression of disease severity in liver cirrhosis or HCC. In conclusion; interferon-γ and interleukin-10 gene polymorphisms are not predictors of disease progression in patients with chronic hepatitis C (Genotype-4).
authors.affiliation: Clinical and Chemical Pathology Department, Faculty of medicine, Cairo University, Egypt E-mail : hendshousha@yahoo.com.
authors.forename: Nermine Ahmed
authors.initials: NA
authors.lastname: Bahgat
authors.forename: Manal Mohamed
authors.initials: MM
authors.lastname: Kamal
authors.forename: Ashraf Omar
authors.initials: AO
authors.lastname: Abdelaziz
authors.forename: Mohamed Ahmed
authors.initials: MA
authors.lastname: Mohye
authors.forename: Hend Ibrahim
authors.initials: HI
authors.lastname: Shousha
authors.forename: Mae Mohamed
authors.initials: MM
authors.lastname: ahmed
authors.forename: Tamer Mahmoud
authors.initials: TM
authors.lastname: Elbaz
authors.forename: Mohamed Mahmoud
authors.initials: MM
authors.lastname: Nabil
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008103:Liver Cirrhosis / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012720:Severity of Illness Index
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma