title: [Contamination level of aflatoxin B1 in lotus seeds rapid screening by indirect competitive ELISA method].
abstract: A simple and cost-effective indirect competitive enzyme-linked immune sorbent assay (ic-ELISA) was developed to rapidly screen the content of aflatoxin B1 (AFB1) in lotus seeds, and the results were confirmed by ultra-fast liquid chromatography-tandem mass spectrometry( UFLC-MS/MS). Matrix-matched calibration expressed a good linearity ranging from 0. 171 to 7. 25 µg · L(-1) for AFB, with R2 > 0.978. The medium inhibitory concentration( IC50 ) for AFB1 was 1.29 µg · L(-1), the recovery for AFB1 was 74.73% to 126.9% with RSD < 5%, and the limit of detection (IC10) was 0.128 µg · L(-1). The developed ic-ELSIA method was applied to rapid analysis of AFB, in 20 lotus seeds samples and the results indicated that the contents of AFB, in samples 1-15 were in the range of 1. 19- 115. 3 µg · kg(-1) and in 40% of the samples exceeded the legal limit(5 µg · kg(-1)), while the contamination rate of AFB, in samples 16-20 was 40%. Pearson correlation coefficient(r) reached 0.997 for AFB1 content in the samples detected by ic-ELSIA and UFLC-MS/MS methods. The results proved that the developed ic-ELISA method is simple, sensitive and reliable, and can be used for rapid and high-throughput screening of AFB1 in lotus seeds
authors.forename: Xian-feng
authors.initials: XF
authors.lastname: Chu
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Dou
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Kong
authors.forename: Mei-hua
authors.initials: MH
authors.lastname: Yang
authors.forename: Chong
authors.initials: C
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ouyang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D004340:Drug Contamination; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000070116:Lotus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D016604:Aflatoxin B1