title: Bio-inspired surface modification of PET for cardiovascular applications: Case study of gelatin.
abstract: An aqueous-based bio-inspired approach was applied to chemically bind a bio compatible and cell-interactive gelatin layer on poly(ethylene terephthalate) (PET) for cardiovascular applications. The protein layer was immobilized after an initial surface activation via a dopamine coating. The individual and synergetic effect of the dopamine deposition procedure and the substrate nature (pristine versus plasma-treated) was investigated via XPS, AFM, SEM and contact angle measurements. Dependent on the applied parameters, the post dopamine coating presented various surface roughnesses ranging between 96 nm and 210 nm. Subsequent gelatin immobilization mostly induced a smoothening effect, but the synergetic influence of the deposition protocol and plasma treatment resulted in different gelatin conformations. In addition, a comprehensive comparative study between chemically-modified (via dopamine) and physically-modified (physisorption) PET with gelatin was developed within the present study. All investigated samples were submitted to preliminary haemocompatibility tests, which clearly indicated the direct link between blood platelet behaviour and final protein arrangement.
authors.affiliation: Polymer Chemistry and Biomaterials Research (PBM) Group, Ghent University, Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium.
authors.forename: E Diana
authors.initials: ED
authors.lastname: Giol
authors.affiliation: Centre of Microsystems Technology (CMST), IMEC and Ghent University, Technologiepark 914A, B-9052 Ghent-Zwijnaarde, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Schaubroeck
authors.affiliation: Laboratory for Radiopharmacy, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
authors.forename: Ken
authors.initials: K
authors.lastname: Kersemans
authors.affiliation: Laboratory for Radiopharmacy, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: De Vos
authors.affiliation: Polymer Chemistry and Biomaterials Research (PBM) Group, Ghent University, Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Van Vlierberghe
authors.affiliation: Polymer Chemistry and Biomaterials Research (PBM) Group, Ghent University, Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium. Electronic address: Peter.Dubruel@UGent.be.
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
mesh_terms: D001792:Blood Platelets / Q000166:cytology; D002318:Cardiovascular Diseases / Q000628:therapy; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible; D004298:Dopamine / Q000008:administration & dosage; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049268:Positron-Emission Tomography; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D005780:Gelatin; D004298:Dopamine
keywords: Cardiovascular; Dopamine; Gelatin; Haemocompatibility; Platelet; Poly(ethylene terephthalate)