title: Insulin sensitivity index (ISI0, 120) potentially linked to carbon isotopes of breath CO2 for pre-diabetes and type 2 diabetes.
abstract: New strategies for an accurate and early detection of insulin resistance are important to delay or prevent the acute onset of type 2 diabetes (T2D). Currently, insulin sensitivity index (ISI0,120) is considered to be a viable invasive method of whole-body insulin resistance for use in clinical settings in comparison with other invasive sensitivity indexes like homeostasis model assessment (HOMA), and quantitative insulin sensitivity check index (QUICKI). To investigate how these sensitivity indexes link the (13)C/(12)C-carbon isotopes of exhaled breath CO2 to pre-diabetes (PD) and type 2 diabetes in response to glucose ingestion, we studied excretion dynamics of (13)C/(12)C-isotopic fractionations of breath CO2. Here, we show that (13)C/(12)C-isotope ratios of breath CO2 were well correlated with blood glucose, insulin, glycosylated-hemoglobin as well as with HOMA-IR and 1/QUICKI. Conversely, the strongest correlation was observed between 1/ISI0,120 and breath CO2 isotopes. Consequently, we determined several optimal diagnostic cut-off points of 1/ISI0,120 and (13)CO2/(12)CO2-isotope ratios to distinctively track the evolution of PD prior to the onset of T2D. Our findings suggest that isotopic breath CO2 is a novel method for accurate estimation of ISI0,120 and thus may open new perspectives into the isotope-specific non-invasive evaluation of insulin resistance for large-scale real-time diabetes screening purposes.
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata-700098, India.
authors.forename: Chiranjit
authors.initials: C
authors.lastname: Ghosh
authors.affiliation: Department of Medicine, Vivekananda Institute of Medical Sciences, 99 Sarat Bose Road, Kolkata-700027, India.
authors.forename: Prabuddha
authors.initials: P
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Medicine, Raipur Institute of Medical Sciences, Raipur-492006, Chhattisgarh, India.
authors.forename: Shibendu
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, JD Block, Sector III, Kolkata-700098, India.
authors.forename: Manik
authors.initials: M
authors.lastname: Pradhan
mesh_terms: D000328:Adult; D019540:Area Under Curve; D001786:Blood Glucose / Q000032:analysis; D001944:Breath Tests; D002245:Carbon Dioxide / Q000737:chemistry* / Q000378:metabolism; D002247:Carbon Isotopes / Q000737:chemistry; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D011236:Prediabetic State / Q000175:diagnosis* / Q000473:pathology; D012372:ROC Curve
chemical_list: D001786:Blood Glucose; D002245:Carbon Dioxide; D002247:Carbon Isotopes; D005947:Glucose; D006442:Glycated Hemoglobin; D007328:Insulin