title: High-speed MALDI MS/MS imaging mass spectrometry using continuous raster sampling.
abstract: A matrix-assisted laser desorption/ionization time of flight/time of flight tandem mass spectrometer (MALDI TOF/TOF) has been used for high-speed precursor/fragment ion transition image acquisition. High-throughput analysis is facilitated by an Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz, a high digitizer acquisition rate (up to 50 pixels/s), and continuous laser raster sampling. MS/MS experiments are enabled through the use of a precision timed ion selector, second source acceleration, and a dedicated collision cell. Continuous raster sampling is shown here to facilitate rapid MS/MS ion image acquisition from thin tissue sections for the drug rifampicin and for a common kidney lipid, SM4s(d18:1/24:1). The ability to confirm the structural identity of an analyte as part of the MS/MS imaging experiment is an essential part of the analysis. Additionally, the increase in sensitivity and specificity afforded by an MS/MS approach is highly advantageous, especially when interrogating complex chemical environments such as those in biological tissues. Herein, we report continuous laser raster sampling TOF/TOF imaging methodologies which demonstrate 8 to 14-fold increases in throughput compared with existing MS/MS instrumentation, an important advantage when imaging large areas on tissues.
authors.affiliation: Department of Biochemistry, Vanderbilt University, Nashville, TN, 37232.|Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37232.
authors.forename: Boone M
authors.initials: BM
authors.lastname: Prentice
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, TN, 37232.|Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37232.
authors.forename: Chad W
authors.initials: CW
authors.lastname: Chumbley
authors.affiliation: Department of Biochemistry, Vanderbilt University, Nashville, TN, 37232.|Department of Chemistry, Vanderbilt University, Nashville, TN, 37232.|Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, TN, 37232.|Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, 37232.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D006651:Histocytochemistry; D007668:Kidney / Q000737:chemistry; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008055:Lipids; D004364:Pharmaceutical Preparations
keywords: MALDI; MS/MS; TOF/TOF; continuous raster; drug imaging; imaging; lipid imaging