title: Effects of high-intensity treadmill training on timeliness and plasticity expression of irisin in mice.
abstract: OBJECTIVE
  The purpose of this study was to investigate effects of high-intensity aerobic exercise training on timeliness and plasticity expression of irisin in mice and change of FNDC5, ACCβ expression, and to explore possible ways to influence its mechanism of fatty acid metabolism.
  
  
  METHODS
  Adult male mice of specific pathogen-free grade [Kunming mice, (20 ± 2) g] are randomly divided into 4 groups. Wherein the first group is immediately after one-time exercise groups: including control group (CN group 1), 0.5 h exercise group (group 2), 1 h exercise group (group 3), 1.5 h exercise group (group 4) and 2 h exercise group (group 5), each for 10. The second group is rest after one-time 60 min exercise groups: including control group (CN group 1), rest 20 min groups (groups 2), rest 40 min group (group 3), rest 60 min group (groups 4), rest 80 min group (group 5), each for 10. Third group is immediately after long-term exercise groups: including the control group (CN group 1), 0.5 h exercise group (group 2), 1 h exercise group (group 3), 1.5 h exercise group (group 4) and 2 h exercise group (group 5), each for 10. The fourth group is rest after long-term 60 min exercise group: including control group (CN group 1), rest 20 min group (group 2), rest 40 min group (group 3), rest 60 min group (4 groups) and rest 80 min groups (5 groups), each for 10.
  
  
  RESULTS
  With the extension of a one-time high-intensity exercise time, the mouse FNDC5 protein, P-ACCβ / ACCβ ratio showed fluctuations, and opposite trends between the two, its turning points are 1.5 h; FNDC5 protein and P-ACCβ / ACCβ ratio with long-term exercise in mice at different time produce adaptability; the regulation of exercise induced irisin timeliness and plasticity reflected after a long-term exercise irisin expression in serum showed a steady decline in trend and return to normal levels, compared to a one-time exercise, expression of irisin is more stable.
  
  
  CONCLUSIONS
  With the high-intensity exercise a one-time extension of time, the mouse FNDC5 proteins, P-ACCβ / ACCβ ratio showed fluctuations, and both changes in the opposite trend, its turning points are 1.5 h; the long-term exercise can produce FNDC5 proteins, P-ACCβ / ACCβ ratios adaptable, more stable expression of the irisin curve after long-term exercise compared to a one-time exercise.
authors.affiliation: College of Health Sciences, Wuhan Sports University, Wuhan, China. liujun@wipe.edu.cn.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: X Y
authors.initials: XY
authors.lastname: Cui
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Yang
authors.forename: W
authors.initials: W
authors.lastname: Gao
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: Y C
authors.initials: YC
authors.lastname: Dong
authors.forename: X J
authors.initials: XJ
authors.lastname: Kou
mesh_terms: D000818:Animals; D000066670:Cell Plasticity / Q000502:physiology*; D005080:Exercise Test / Q000379:methods*; D005353:Fibronectins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000379:methods / Q000502:physiology*; D013997:Time Factors
chemical_list: C571428:FNDC5 protein, mouse; D005353:Fibronectins