title: The p16INK4a Antibody Immobilization Method for Immonosensor Application.
abstract: BACKGROUND
  The p16INK4a is a protein that expressed in Liquid-based cervical cytology specimens and has been proved link to cervical cancer. The p16INK4a could be detection by piezoelectric immunosensor and the immobilization of the p16INK4a antibody influence the sensitivity of the piezoelectric immunosensor.
  
  
  METHODS
  5μL mouse polyclonal antibody against p16INK4a was bound onto the surface of immonosensor through two methods. (directly immobilized method; protein A method). Absorb of the p16INK4a antibody on the surface of immonosensor caused a shift in the resonant frequency of the immunosensor and The frequency changes recorded showed a better reproducibility. The activity of the immobilization antibody with the directly method and protein A method was tested with p16INK4a antigen.
  
  
  RESULTS
  The resonant frequency for different antibody immobilization methods were different, and the sensitivity for p16INK4a detection also different.
  
  
  CONCLUSIONS
  The protein A method was found to be much more better than the directly method for the immobilization of the p16INK4A antibody on the gold electrode of the quartz crystal for cervical lesion detection. The Protein A method created more reproducible and stable immobilization antibody layers with p16INK4A antigen.
authors.affiliation: Frequency Control Laboratory, School of Automation and Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan, China E-mail : yang9707@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Xian-He
authors.initials: XH
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000097:blood / Q000276:immunology*; D055088:Early Detection of Cancer; D004566:Electrodes; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D011791:Quartz / Q000737:chemistry* / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000276:immunology / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D019941:Cyclin-Dependent Kinase Inhibitor p16; D013205:Staphylococcal Protein A; D011791:Quartz; D006046:Gold