title: Facile Preparation of Core-Shell Magnetic Metal-Organic Framework Nanoparticles for the Selective Capture of Phosphopeptides.
abstract: In regard to the phosphoproteome, highly specific and efficient capture of heteroideous kinds of phosphopeptides from intricate biological sample attaches great significance to comprehensive and in-depth phosphorylated proteomics research. However, until now, it has been a challenge. In this study, a new-fashioned porous immobilized metal ion affinity chromatography (IMAC) material was designed and fabricated to promote the selectivity and detection limit for phosphopeptides by covering a metal-organic frameworks (MOFs) shell onto Fe3O4 nanoparticles, taking advantage of layer-by-layer method (the synthesized nanoparticle denoted as Fe3O4@MIL-100 (Fe)). The thick layer renders the nanoparticles with perfect hydrophilic character, super large surface area, large immobilization of the Fe(3+) ions and the special porous structure. Specifically, the as-synthesized MOF-decorated magnetic nanoparticles own an ultra large surface area which is up to 168.66 m(2) g(-1) as well as two appropriate pore sizes of 1.93 and 3.91 nm with a narrow grain-size distribution and rapid separation under the magnetic circumstance. The unique features vested the synthesized nanoparticles an excellent ability for phosphopeptides enrichment with high selectivity for β-casein (molar ratio of β-casein/BSA, 1:500), large enrichment capacity (60 mg g(-1)), low detection limit (0.5 fmol), excellent phosphopeptides recovery (above 84.47%), fine size-exclusion of high molecular weight proteins, good reusability, and desirable batch-to-batch repeatability. Furthermore, encouraged by the experimental results, we successfully performed the as-prepared porous IMAC nanoparticle in the specific capture of phosphopeptides from the human serum (both the healthy and unhealthy) and nonfat milk, which proves itself to be a good candidate for the enrichment and detection of the low-abundant phosphopeptides from complicated biological samples.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yiman
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Qian
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D003460:Crystallization / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009930:Organic Chemicals / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D058185:Magnetite Nanoparticles; D009930:Organic Chemicals; D010748:Phosphopeptides
keywords: magnetic nanoparticles; mass spectrum; metal−organic framework (MOF); phosphorylated peptides; size-exclusion