title: Separation, determination and antifungal activity test of the products from a new Bacillus amyloliquefaciens.
abstract: A new Bacillus amyloliquefaciens named ZJU-2011 was discovered, and the culture supernatant showed a strong inhibitory effect against Candida albicans. In this study, a novel method was developed to purify the antifungal compounds in high purity. The obtained products were analysed by high performance liquid chromatography and proven to be of high purity. Mass spectrometry showed that the molecular weights of the two bioactive components were 270 and 288, respectively, and their structures were determined to be bacilysin and chlorotetaine by using (1)H and (13)C nuclear magnetic resonance spectroscopy. To the best of our knowledge, this is the first time that B. amyloliquefaciens has been reported to produce bacilysin and chlorotetaine simultaneously. The minimum inhibitory concentration of chlorotetaine against six common fungal pathogens were determined to be in the range of 1.8-7.8 μg/mL.
authors.affiliation: a Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University , Hangzhou 310027 , P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University , Hangzhou 310027 , P.R. China.|b Zhejiang Key Laboratory of Antifungal Drugs , Taizhou , P.R. China.
authors.forename: Mian-Bin
authors.initials: MB
authors.lastname: Wu
authors.affiliation: b Zhejiang Key Laboratory of Antifungal Drugs , Taizhou , P.R. China.
authors.forename: Zheng-Jie
authors.initials: ZJ
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University , Hangzhou 310027 , P.R. China.
authors.forename: Jian-Pin
authors.initials: JP
authors.lastname: Lin
authors.affiliation: a Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University , Hangzhou 310027 , P.R. China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Yang
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification*; D001407:Bacillus / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure
chemical_list: D000935:Antifungal Agents; D004151:Dipeptides; C006674:bacilysin
keywords: Bacillus amyloliquefaciens; antifungal; bacilysin; chlorotetaine; isolate