title: Burkholderia gut symbionts enhance the innate immunity of host Riptortus pedestris.
abstract: The relation between gut symbiosis and immunity has been reported in various animal model studies. Here, we corroborate the effect of gut symbiont to host immunity using the bean bug model. The bean bug, Riptortus pedestris, is a useful gut symbiosis model due to the monospecific gut symbiont, genus Burkholderia. To examine the effect of gut symbiosis to host immunity, we generated the gut symbiont-harboring (symbiotic) insect line and the gut symbiont-lacking (aposymbiotic) insect line. Upon bacterial challenges, the symbiotic Riptortus exhibited better survival than aposymbiotic Riptortus. When cellular immunity was inhibited, the symbiotic Riptortus still survived better than aposymbioic Riptortus, suggesting stronger humoral immunity. The molecular basis of the strong humoral immunity was further confirmed by the increase of hemolymph antimicrobial activity and antimicrobial peptide expression in the symbiotic insects. Taken together, our data clearly demonstrate that Burkhoderia gut symbiont positively affect the Riptortus systemic immunity.
authors.affiliation: Department of Microbiology, Kosin University College of Medicine, Busan 602-703, South Korea.
authors.forename: Jiyeun Kate
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Jun Beom
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Ye Rang
authors.initials: YR
authors.lastname: Huh
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Ho Am
authors.initials: HA
authors.lastname: Jang
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Yoo
authors.affiliation: Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea. Electronic address: brlee@pusan.ac.kr.
authors.forename: Bok Luel
authors.initials: BL
authors.lastname: Lee
mesh_terms: D000818:Animals; D019117:Burkholderia / Q000276:immunology*; D048168:Escherichia coli K12 / Q000276:immunology; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology; D006458:Hemolymph / Q000276:immunology; D020062:Heteroptera / Q000276:immunology* / Q000382:microbiology*; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013559:Symbiosis
keywords: Antimicrobial peptide; Burkholderia symbiont; Gut symbiosis; Innate immunity; Riptortus pedestris