title: Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice.
abstract: Objective. Intestinal ischemia reperfusion (II/R) injury plays a critical role in remote organ dysfunction, such as lung injury, which is associated with nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. In the present study, we tested whether ginsenoside Rb1 attenuated II/R induced lung injury by Nrf2/HO-1 pathway. Methods. II/R injury was induced in male C57BL/6J mice by 45 min of superior mesenteric artery (SMA) occlusion followed by 2 hours of reperfusion. Ginsenoside Rb1 was administrated prior to reperfusion with or without ATRA (all-transretinoic acid, the inhibitor of Nrf2/ARE signaling pathway) administration before II/R. Results. II/R induced lung histological injury, which is accompanied with increased levels of malondialdehyde (MDA), interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) α but decreased levels of superoxide dismutase (SOD) and IL-10 in the lung tissues. Ginsenoside Rb1 reduced lung histological injury and the levels of TNF-α and MDA, as well as wet/dry weight ratio. Interestingly, the increased Nrf2 and HO-1 expression induced by II/R in the lung tissues was promoted by ginsenoside Rb1 treatment. All these changes could be inhibited or prevented by ATRA. Conclusion. Ginsenoside Rb1 is capable of ameliorating II/R induced lung injuries by activating Nrf2/HO-1 pathway.
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Qing-tao
authors.initials: QT
authors.lastname: Meng
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wating
authors.initials: W
authors.lastname: Su
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Lei
authors.affiliation: Department of Anesthesiology, The University of Hong Kong, Pok Fu Lam, Hong Kong ; Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, China.
authors.forename: Zhengyuan
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Zhong-yuan
authors.initials: ZY
authors.lastname: Xia
mesh_terms: D000818:Animals; D036145:Ginsenosides / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007422:Intestines / Q000473:pathology*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D036145:Ginsenosides; D015850:Interleukin-6; D051267:NF-E2-Related Factor 2; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D008315:Malondialdehyde; D014212:Tretinoin; C442759:ginsenoside Rb1; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase