title: [Effect of PM2.5 on oxidative stress-JAK/STAT signaling pathway of human bronchial epithelial cells].
abstract: OBJECTIVE
  To investigate the regulation of oxidative stress-JAK/STAT signaling pathway on cytokines in human bronchial epithelial cells induced by PM2.5.
  
  
  METHODS
  Bronchial epithelial cells 16HBE were cultured using traditional invasive methods. Two aspects were explored, one is the relationship among PM2.5, oxidative stress, JAK/STAT signaling pathway. There were the following groups, control group, N-acetyl-L-cysteine (NAC, 5 mmol/L) group, 50 μg/ml PM2.5 group, 100 μg/ml PM2.5 group, 50 μg/ml PM2.5 + 5 mmol/L NAC group, 100 μg/ml PM2.5 + 5 mmol/L NAC group. Intracellular ROS levels and the gene expression of JAK2 and STAT3 were detected after all groups were exposed for 24 h. The other one is to explore the relationship among PM2.5, JAK/STAT signaling pathway and cytokine. The groups were arranged the following, control group, 6 μmol/L AG490 group, 100 μg/ml PM2.5 group, 6 μmol/L AG490 + 100 μg/ml PM2.5 group. The level of IL-6 was determined after all groups were exposed for 24h.
  
  
  RESULTS
  After 24 h exposure, intracellular ROS levels and the gene expression of JAK2 and STAT3 in 50 μg/ml PM2.5, 100 μg/ml PM2.5 exposure group were higher than the control group. Intracellular ROS levels and the gene expression of JAK2 and STAT3 in PM2.5 + NAC protection group were lower than the respective PM2.5 exposure group, the differences were statistically significant (P < 0.05). IL-6 levels of cellular supernatant in 6 μmol/L AG490 + 100 μg/ml PM2.5 group were lower than 100 μg/M PM2. group, the differences were statistically significant ( P < 0.05).
  
  
  CONCLUSIONS
  PM2.5 could cause oxidative damage on bronchial epithelial cells, and then regulate JAK/STAT signaling pathway and relative cytokines of epithelial cells through oxidative stress.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Xu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ma
authors.forename: Feifei
authors.initials: F
authors.lastname: Ping
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000111:Acetylcysteine; D016207:Cytokines; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D053614:Janus Kinase 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D020032:Tyrphostins
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; C507924:JAK2 protein, human; D053614:Janus Kinase 2; D000111:Acetylcysteine