title: Development and Validation of Two Screening Assays for the Hepatitis C Virus NS3 Q80K Polymorphism Associated with Reduced Response to Combination Treatment Regimens Containing Simeprevir.
abstract: Persons with hepatitis C virus (HCV) genotype 1a (GT1a) infections harboring a baseline Q80K polymorphism in nonstructural protein 3 (NS3) have a reduced virologic response to simeprevir in combination with pegylated interferon-alfa and ribavirin. We aimed to develop, validate, and freely disseminate an NS3 clinical sequencing assay to detect the Q80K polymorphism and potentially other HCV NS3 drug resistance mutations. HCV RNA was extracted from frozen plasma using a NucliSENS easyMAG automated nucleic acid extractor, amplified by nested reverse transcription-PCR, and sequenced using Sanger and/or next-generation (MiSeq) methods. Sanger chromatograms were analyzed using in-house software (RECall), and nucleotide mixtures were called automatically. MiSeq reads were iteratively mapped to the H77 reference genome, and consensus NS3 sequences were generated with nucleotides present at >20% called as mixtures. The accuracy, precision, and sensitivity for detecting the Q80K polymorphism were assessed in 70 samples previously sequenced by an external laboratory. A comparison of the sequences generated by the Sanger and MiSeq methods with those determined by an external lab revealed >98.5% nucleotide sequence concordance and zero discordant calls of the Q80K polymorphism. The results were both highly repeatable and reproducible (>99.7% nucleotide concordance and 100% Q80K concordance). The limits of detection (>2 and ∼5 log10 IU/ml for the Sanger and MiSeq assays, respectively) are sufficiently low to allow genotyping in nearly all chronically infected treatment-naive persons. No systematic bias in the under- or overamplification of minority variants was observed. Coinfection with other viruses (e.g., HIV and hepatitis B virus [HBV]) did not affect the assay results. The two independent HCV NS3 sequencing assays with the automated analysis procedures described here are useful tools to screen for the Q80K polymorphism and other HCV protease inhibitor drug resistance mutations.
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: C K S
authors.initials: CK
authors.lastname: Chui
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: W W Y
authors.initials: WW
authors.lastname: Dong
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: J B
authors.initials: JB
authors.lastname: Joy
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: A F Y
authors.initials: AF
authors.lastname: Poon
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: W Y
authors.initials: WY
authors.lastname: Dong
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Mo
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: C K
authors.initials: CK
authors.lastname: Woods
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Beatty
authors.affiliation: Clinical Affairs, Janssen Inc., North York, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Hew
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada prharrigan@cfenet.ubc.ca cbrumme@cfenet.ubc.ca.
authors.forename: P R
authors.initials: PR
authors.lastname: Harrigan
authors.affiliation: BC Centre for Excellence in HIV/AIDS, Vancouver, British Columbia, Canada prharrigan@cfenet.ubc.ca cbrumme@cfenet.ubc.ca.
authors.forename: C J
authors.initials: CJ
authors.lastname: Brumme
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004351:Drug Resistance; D060005:Genotyping Techniques / Q000379:methods*; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000821:virology*; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D050505:Mutant Proteins / Q000235:genetics; D020125:Mutation, Missense; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000069616:Simeprevir / Q000494:pharmacology*; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000998:Antiviral Agents; D050505:Mutant Proteins; D000069616:Simeprevir; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases