title: Deep sequencing analysis of microRNA expression in human melanocyte and melanoma cell lines.
abstract: Melanoma is a type of skin cancer that is highly aggressive, and is considered the most deadly of all skin cancers. Currently, there are no effective therapies for melanomas once they undergo metastasis. MicroRNAs (miRNAs) are small, single-stranded, non-coding RNA molecules that can post-transcriptionally regulate gene expression. They have been reported to be associated with the occurrence of many diseases, including human melanoma. However, the mechanisms by which miRNAs exert their effects remain unclear; therefore, a systematic analysis of the miRNAome in human melanoma is necessary. We investigated the miRNAome in human melanocyte and melanoma cell lines using high-throughput RNA sequencing. We identified a group of dysregulated miRNAs by comparing the miRNA expression profiles among the melanoma cell lines. Target genes of these miRNAs encode proteins whose functions are associated with the cell cycle and apoptosis. Gene networks were built to investigate the interactions of genes during melanoma progression. We identified that the key genes that regulate melanoma cell proliferation were regulated by miRNAs. In summary, our investigation of the human melanoma miRNAome using high-throughput sequencing revealed a number of previously unreported miRNAs associated with malignant progression of melanoma. Our findings add to existing knowledge regarding the mechanisms of melanoma development.
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Ding
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shaobin
authors.initials: S
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Biology, Hebei Academy of Sciences, Shijiazhuang 050081, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Junyun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Dermatology, General Hospital of People's Liberation Army, Beijing 100853, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Youhui
authors.initials: Y
authors.lastname: Deng
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Dermatology, General Hospital of People's Liberation Army, Beijing 100853, China. Electronic address: luckhua301@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: fangxd@big.ac.cn.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Fang
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D018450:Disease Progression; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008544:Melanocytes / Q000378:metabolism*; D008545:Melanoma / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012878:Skin Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm
keywords: High-throughput RNA sequencing; Melanoma; MicroRNAs; PI3K-AKT signaling pathway; TGF-beta signaling pathway