title: [Microgeographic and temporal genetic changes of Aedes aegypti from Medellín, Colombia].
abstract: BACKGROUND
  Aedes aegypti populations may experience changes in abundance and genetic diversity in addition to changes in their evolutionary capability to respond to vector control. The knowledge on the changes in genetic variation on a spatio-temporal scale improves the epidemiological understanding of dengue and supports the appropriate and timely design of vector control strategies.
  
  
  OBJECTIVE
  To assess the genetic changes, diversity and gene flow in six microgeographical populations of Ae. aegypti in Medellín for different epidemiological periods of dengue.
  
  
  METHODS
  A total of 255 specimens from six different neighborhoods in Medellín were used to assess variations in the CO1 mtDNA haplotype composition, diversity and genetic differentiation for an epidemic period (2010) and an endemic period (2012).
  
  
  RESULTS
  Two groups of highly differentiated haplotypes were present in both periods, and a high-frequency haplotype was assessed for all neighborhoods. The highest haplotype diversity was recorded in 2012, but the maximum nucleotide diversity was recorded in 2010. No significant correlation between genetic and geographic distances was observed.
  
  
  CONCLUSIONS
  The genetic composition of Ae. aegypti varies over time without a predictable pattern. In addition, the presence of a high-frequency haplotype in both periods could indicate a persistent variation adapted to vector control. However, the simultaneous movement of highly differentiated CO1 haplotypes compatible with multiple introductions suggests that different gene pools would be suitable for transmission. These results are consistent with mosquito dispersion due to human activities, which would enable the rapid spread of the virus during epidemics in Medellin.
authors.affiliation: Grupo de Entomología, Facultad de Medicina, Universidad de Antioquia.
authors.forename: Jorge Mario
authors.initials: JM
authors.lastname: Cadavid
authors.affiliation: Grupo de Entomología, Facultad de Medicina, Universidad de Antioquia.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rúa
authors.affiliation: Grupo de Genética Molecular, Instituto de Biología, Universidad de Antioquia.
authors.forename: Omer
authors.initials: O
authors.lastname: Campo
authors.affiliation: Grupo de Genética Molecular, Instituto de Biología, Universidad de Antioquia.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Bedoya
authors.affiliation: Grupo de Genética Molecular, Instituto de Biología, Universidad de Antioquia.
authors.forename: Winston
authors.initials: W
authors.lastname: Rojas
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D003105:Colombia; D003710:Demography; D017344:Genes, Insect; D014644:Genetic Variation; D005843:Geography; D006239:Haplotypes