title: Effects of sevoflurane on ventilator induced lung injury in a healthy lung experimental model.
abstract: OBJECTIVE
  Ventilator-induced lung injury (VILI) causes a systemic inflammatory response in tissues, with an increase in IL-1, IL-6 and TNF-α in blood and tissues. Cytoprotective effects of sevoflurane in different experimental models are well known, and this protective effect can also be observed in VILI. The objective of this study was to assess the effects of sevoflurane in VILI.
  
  
  METHODS
  A prospective, randomized, controlled study was designed. Twenty female rats were studied. The animals were mechanically ventilated, without sevoflurane in the control group and sevoflurane 3% in the treated group (SEV group). VILI was induced applying a maximal inspiratory pressure of 35 cmH2O for 20 min without any positive end-expiratory pressure for 20 min (INJURY time). The animals were then ventilated 30 min with a maximal inspiratory pressure of 12 cmH2O and 3 cmH2O positive end-expiratory pressure (time 30 min POST-INJURY), at which time the animals were euthanized and pathological and biomarkers studies were performed. Heart rate, invasive blood pressure, pH, PaO2, and PaCO2 were recorded. The lung wet-to-dry weight ratio was used as an index of lung edema.
  
  
  RESULTS
  No differences were found in the blood gas analysis parameters or heart rate between the 2 groups. Blood pressure was statistically higher in the control group, but still within the normal clinical range. The percentage of pulmonary edema and concentrations of TNF-α and IL-6 in lung tissue in the SEV group were lower than in the control group.
  
  
  CONCLUSIONS
  Sevoflurane attenuates VILI in a previous healthy lung in an experimental subclinical model in rats.
authors.affiliation: Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, España. Electronic address: antonromero@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Romero
authors.affiliation: Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, España.
authors.forename: A
authors.initials: A
authors.lastname: Moreno
authors.affiliation: Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, España.
authors.forename: J
authors.initials: J
authors.lastname: García
authors.affiliation: Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, España.
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.affiliation: Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, España.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, España.
authors.forename: J
authors.initials: J
authors.lastname: García
mesh_terms: D000818:Animals; D008168:Lung; D008962:Models, Theoretical; D011175:Positive-Pressure Respiration / Q000009:adverse effects; D011446:Prospective Studies; D051381:Rats; D055397:Ventilator-Induced Lung Injury / Q000209:etiology
keywords: Anestesia; Anesthesia; Daño pulmonar; Inflamación; Inflammation; Lung injury; Mechanical ventilation; Sevoflurane; Sevoflurano; Ventilación mecánica