title: Plant-Derived Tick Repellents Activate the Honey Bee Ectoparasitic Mite TRPA1.
abstract: We have identified and characterized the TRPA1 channel of Varroa destructor (VdTRPA1), a major ectoparasitic mite of honey bee. One of the two VdTRPA1 isoforms, VdTRPA1L, was activated by a variety of plant-derived compounds, including electrophilic compounds, suggesting that chemical activation profiles are mostly shared between arthropod TRPA1 channels. Nevertheless, carvacrol and α-terpineol activated VdTRPA1L but not a honey bee noxious-stimuli-sensitive TRPA, AmHsTRPA, and Drosophila melanogaster TRPA1. Activation of VdTRPA1L in D. melanogaster taste neurons by the above compounds was sufficient to modify the gustatory behaviors. Carvacrol and α-terpineol repelled V. destructor in a laboratory assay, and α-terpineol repressed V. destructor entry for reproduction into the brood cells in hives. Understanding the functions of parasite TRP channels not only gives clues about the evolving molecular and cellular mechanisms of parasitism but also helps in the development of control methods.
authors.affiliation: Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China.
authors.forename: Guangda
authors.initials: G
authors.lastname: Peng
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Kashio
authors.affiliation: Nagaragawa Research Center, API Co., Ltd., 692-3, Nagara, Gifu 502-0071, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Morimoto
authors.affiliation: Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China.
authors.forename: Tianbang
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Department of Physiological Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
authors.affiliation: Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China. Electronic address: tatsuhiko.kadowaki@xjtlu.edu.cn.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kadowaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000378:metabolism*; D001516:Bees / Q000378:metabolism; D001522:Behavior, Animal / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000081007:Cymenes; D004331:Drosophila melanogaster / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D057809:HEK293 Cells; D006801:Humans; D007300:Insect Control; D008969:Molecular Sequence Data; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D050051:Transient Receptor Potential Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057077:Varroidae / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D000081007:Cymenes; D039821:Monoterpenes; D020033:Protein Isoforms; D050051:Transient Receptor Potential Channels; C016775:alpha-terpineol; C073316:carvacrol; D002118:Calcium