title: Cellular stress induces cap-independent alpha-enolase/MBP-1 translation.
abstract: Myc promoter-binding protein-1 (MBP-1) is a shorter protein variant of the glycolytic enzyme alpha-enolase. Although several lines of evidence indicate that MBP-1 acts as a tumor suppressor, the cellular mechanisms and signaling pathways underlying MBP-1 expression still remain largely elusive. To dissect these pathways, we used the SkBr3 breast cancer cell line and non-tumorigenic HEK293T cells ectopically overexpressing alpha-enolase/MBP-1. Here, we demonstrate that induced cell stresses promote MBP-1 expression through the AKT/PERK/eIF2α signaling axis. Our results contribute to shedding light on the molecular mechanisms underlying MBP-1 expression in non-tumorigenic and cancer cells.
authors.affiliation: Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National Research Council (CNR), Palermo, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Maranto
authors.affiliation: Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National Research Council (CNR), Palermo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Perconti
authors.affiliation: Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Contino
authors.affiliation: Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National Research Council (CNR), Palermo, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rubino
authors.affiliation: Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National Research Council (CNR), Palermo, Italy; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Italy. Electronic address: salvatore.feo@unipa.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Feo
authors.affiliation: Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National Research Council (CNR), Palermo, Italy. Electronic address: agata.giallongo@ibim.cnr.it.
authors.forename: Agata
authors.initials: A
authors.lastname: Giallongo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D019892:eIF-2 Kinase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C088830:DENND4A protein, human; D004268:DNA-Binding Proteins; D015852:Eukaryotic Initiation Factor-2; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; C494918:AKT1 protein, human; C460640:EIF2AK3 protein, human; D051057:Proto-Oncogene Proteins c-akt; D019892:eIF-2 Kinase; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase
keywords: Alternative translation; Breast cancer; Endoplasmic reticulum stress; Myc promoter-binding protein-1