title: [Over-expression of transcription factor 21 inhibits the proliferation and migration and promotes apoptosis of SMMC-7721 cells].
abstract: OBJECTIVE
  To explore the impact of transcription factor 21 (TCF21) gene on proliferation, migration and apoptosis in SMMC-7721 hepatocellular carcinoma cell line and the related mechanism.
  
  
  METHODS
  Using Lipofectamine™2000, we stably transfected pcDNA3.1⁺TCF21 and pcDNA3.1⁺ plasmids into SMMC-7721 cells of TCF21 over-expression group and empty vector group, respectively. The untreated cells were set as blank control group. The expression of TCF21 mRNA and protein were investigated by reverse transcription PCR and Western blotting. Cell proliferation ability was detected by MTT assay. Wound healing assay was used to observe cell migration ability. Annexin V-FITC/PI double labeling combined with flow cytometry was applied to determine cell apoptosis rate. Western blotting was performed to examine the expressions of KISS1, P53 and matrix metalloproteinase-9 (MMP-9).
  
  
  RESULTS
  TCF21 was over-expressed in TCF21-transfected SMMC-7721 cells. Compared with the control groups, the TCF21 over-expression group showed relatively weakened proliferation ability and significantly inhibited migration ability as well as the increased apoptosis. Western blotting demonstrated that up-regulated TCF21 raised the expressions of KISS1 and p53, and down-regulated MMP-9 level.
  
  
  CONCLUSIONS
  Tumor-suppressor gene TCF21 could inhibit the proliferation and migration of SMMC-7721 hepatocellular carcinoma cells and promote its apoptosis.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Wu
mesh_terms: D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D059648:Kisspeptins / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D059648:Kisspeptins; D020780:Matrix Metalloproteinase 9; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; C106628:KISS1 protein, human; C491825:TCF21 protein, human