title: In vitro study of endogenous CEST agents at 3 T and 7 T.
abstract: Chemical exchange saturation transfer (CEST) has been an intensive research area in MRI, providing contrast mechanisms for the amplified detection and monitoring of biomarkers and physiologically active molecules. In biological tissues and organs, many endogenous CEST agents coexist, and their CEST effects may overlap. The interpretation of such overlapped CEST effects can be addressed when the individual CEST effects originating from various metabolites are characterized. In this work, we present the in vitro measurements of the CEST effects from endogenous CEST agents that are commonly found in biological tissues and organs, at the external magnetic fields of 3 T and 7 T and under various pH conditions. Together with the proton NMR spectra measured at 11.7 T, these CEST effects have been evaluated in consideration of the chemical exchange rates, chemical shifts, and acidities of the labile protons. Amine protons of small metabolites might not be visible at 3 T, but some of them can be probed at 7 T, wherein their CEST effects may overlap with those from coexisting amide and hydroxyl protons.
authors.affiliation: Center for Advanced Imaging Innovation and Research (CAI2R), Department of Radiology, New York University School of Medicine, New York, NY, USA.|Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, NY, USA.|Department of Chemistry, New York University, New York, NY, USA.
authors.forename: Jae-Seung
authors.identifier: http://orcid.org/0000-0002-8571-0663
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Center for Advanced Imaging Innovation and Research (CAI2R), Department of Radiology, New York University School of Medicine, New York, NY, USA.|Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: Ding
authors.initials: D
authors.lastname: Xia
authors.affiliation: Department of Chemistry, New York University, New York, NY, USA.
authors.forename: Alexej
authors.initials: A
authors.lastname: Jerschow
authors.affiliation: Center for Advanced Imaging Innovation and Research (CAI2R), Department of Radiology, New York University School of Medicine, New York, NY, USA.|Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: Ravinder R
authors.initials: RR
authors.lastname: Regatte
mesh_terms: D000577:Amides / Q000737:chemistry*; D003198:Computer Simulation; D003287:Contrast Media / Q000737:chemistry*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D019047:Phantoms, Imaging; D011522:Protons
chemical_list: D000577:Amides; D003287:Contrast Media; D011522:Protons
keywords: acidity; chemical exchange; chondroitin sulfate; creatine; glutamate; glycogen; myo-inositol; saturation transfer