title: [RIBOSOMAL DNA INTERNAL TRANSCRIBED SPACER 2 SEQUENCE AS A PHYLOGENETIC MARKER FOR THE IDENTIFICATION OF TRICHINELLA NEMATODES].
abstract: The results of testing several primer combinations were used to choose an optimal pair for the amplification of the internal transcribed spacer 2 (ITS2) region of ribosomal DNA (direct: Tri58s F 5 CGG TGG ATC RCT TGG CTC GTA CG and reverse: AB28 Rr (CGA CCG CTT ATT GAT ATG C). This pair of primers yields a 900 bp PCR product. Comparative analysis of obtained ITS2 sequences, for 8 Trichinella isolates from different regions of the Russian Federation permits different species and individual genotypes of these parasitic nematodes to be validly distinguished.
authors.forename: I M
authors.initials: IM
authors.lastname: Odoyevskaya
authors.forename: S E
authors.initials: SE
authors.lastname: Spiridonov
mesh_terms: D000818:Animals; D006224:Cricetinae; D018509:DNA, Helminth / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005819:Genetic Markers; D051379:Mice; D010802:Phylogeny; D051381:Rats; D014234:Trichinella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers