title: Clarithromycin regresses endometriotic implants in rat endometriosis model.
abstract: The aim of this study was to investigate the effect of clarithromycin in rat endometriosis and its association with matrix metalloproteinase-9 (MMP-9) expression. After surgical induction of endometriosis, 27 rats were randomised into three groups. Size of endometriotic implants were evalutated and rats in group I (n = 9) were given 100 mg/kg/day of oral clarithromycin, rats in group II (n = 9) were given single 1 mg/kg s.c. injection of leuprolide acetate and rats in group III (n = 9) were not given any medication for 21 days. At the end of 21 days of medication, remaining 23 rats were sacrificed to evaluate morphological and histological features of implants. There was a significant difference between the groups in implant volumes (p = 0.004) before and after medication. Regression of implants were significantly higher in groups I and II than that in control group (p = 0.009 and p = 0.011, respectively). After medication, in group I the implant volume decreased from 62 (12-166) mm(3) to 26 (3-87) mm(3) (p = 0.012) and in group II the volume decreased from 224 (76-1135) mm(3) to 62 (26-101) mm(3) (p = 0.028). There was a significant difference between groups in histopathological score (p = 0.024). The epithelial immunohistochemical score of MMP-9 was significantly lower in group II than that in control group (p = 0.014). In conclusion, clarithromycin regresses endometriotic implants in rats, but not via MMP-9.
authors.affiliation: a Department of Obstetrics and Gynecology , Ankara Kecioren Teaching and Research Hospital , Ankara , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Cavkaytar
authors.affiliation: b Department of Obstetrics and Gynecology , Ankara Etlik Zubeyde Hanim Woman Health Teaching and Research Hospital , Ankara , Turkey.
authors.forename: O L
authors.initials: OL
authors.lastname: Tapisiz
authors.affiliation: b Department of Obstetrics and Gynecology , Ankara Etlik Zubeyde Hanim Woman Health Teaching and Research Hospital , Ankara , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Kiykac Altinbas
authors.affiliation: c Department of Pediatrics Enfectious Diseases , Ankara Gazi University Faculty of Medicine , Ankara , Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Tapisiz
authors.affiliation: d Department of Pathology , Ankara Gazi University Faculty of Medicine , Ankara , Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Erdem
authors.affiliation: b Department of Obstetrics and Gynecology , Ankara Etlik Zubeyde Hanim Woman Health Teaching and Research Hospital , Ankara , Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Goktolga
mesh_terms: D000818:Animals; D017291:Clarithromycin / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004715:Endometriosis / Q000188:drug therapy* / Q000201:enzymology; D005260:Female; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D011500:Protein Synthesis Inhibitors; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat; D017291:Clarithromycin
keywords: Clarithromycin; MMP-9; endometriosis; leuprolide acetate; rat