title: PRESYNAPTIC NETWORKS. Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules.
abstract: Individual cortical neurons can selectively respond to specific environmental features, such as visual motion or faces. How this relates to the selectivity of the presynaptic network across cortical layers remains unclear. We used single-cell-initiated, monosynaptically restricted retrograde transsynaptic tracing with rabies viruses expressing GCaMP6s to image, in vivo, the visual motion-evoked activity of individual layer 2/3 pyramidal neurons and their presynaptic networks across layers in mouse primary visual cortex. Neurons within each layer exhibited similar motion direction preferences, forming layer-specific functional modules. In one-third of the networks, the layer modules were locked to the direction preference of the postsynaptic neuron, whereas for other networks the direction preference varied by layer. Thus, there exist feature-locked and feature-variant cortical networks.
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Wertz
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Stuart
authors.initials: S
authors.lastname: Trenholm
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yonehara
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hillier
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Raics
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Marcus
authors.initials: M
authors.lastname: Leinweber
authors.affiliation: Two-Photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Szalay
authors.affiliation: Max von Pettenkofer-Institute and Gene Center, Ludwig-Maximilians-University Munich, Munich, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Ghanem
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
authors.forename: Georg
authors.initials: G
authors.lastname: Keller
authors.affiliation: Two-Photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Rózsa
authors.affiliation: Max von Pettenkofer-Institute and Gene Center, Ludwig-Maximilians-University Munich, Munich, Germany.
authors.forename: Karl-Klaus
authors.initials: KK
authors.lastname: Conzelmann
authors.affiliation: Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. Department of Ophthalmology, University of Basel, Basel, Switzerland. botond.roska@fmi.ch.
authors.forename: Botond
authors.initials: B
authors.lastname: Roska
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005074:Evoked Potentials, Visual; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D051379:Mice; D009038:Motion; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D059906:Neuroimaging; D017729:Presynaptic Terminals / Q000502:physiology*; D017966:Pyramidal Cells / Q000502:physiology*; D011820:Rabies virus; D059010:Single-Cell Analysis; D014793:Visual Cortex / Q000502:physiology*
chemical_list: D002135:Calcium-Binding Proteins; D008164:Luminescent Proteins