title: MiR-449c inhibits gastric carcinoma growth.
abstract: OBJECTIVE
  Gastric carcinoma (GC) is among the leading causes of cancer-related deaths in China. Growing evidence indicates that dysregulation of miRNAs contributes to GC development. Although it has been shown that miR-449c acts as a tumor suppressor in lung cancer, the role of miR-449c in GC remains unclear.
  
  
  METHODS
  Here, we analyzed miR-449c levels in GC tissues and cell lines by RT-qPCR. We also overexpressed and inhibited miR-449c by transfecting miRNA mimics and antisense oligonucleotides (ASO), respectively. Cell growth was analyzed by MTT assay, and cell apoptosis was evaluated by FACS analysis. MiR-449c target genes were predicted using bioinformatics algorithms and confirmed by a dual luciferase reporter assay.
  
  
  RESULTS
  We detected lower miR-449c levels in GC tissues; the low miR-449c levels correlated with low survival rate. Overexpression of miR-449c inhibited cell growth and promoted apoptosis, while depletion of miR-449c increased cell growth and suppressed apoptosis. Moreover, the 3' UTR of MET, an oncogene that activates tumor cell growth, appeared to be targeted by miR-449c.
  
  
  CONCLUSIONS
  Together, we showed that the reduced miR-449c levels in GC tissues promote GC growth, which possibly contributes to the low survival rate of GC patients. Mechanistically, miR-449c may target MET to suppress GC cell growth.
authors.affiliation: Department of Surgery, Shaoxing Hospital of China Medical University, 1 Huayu Road, Shaoxing 312030, China. Electronic address: xucy15@163.com.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Surgery, Shaoxing Hospital of China Medical University, 1 Huayu Road, Shaoxing 312030, China.
authors.forename: Huangen
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Surgery, Shaoxing Hospital of China Medical University, 1 Huayu Road, Shaoxing 312030, China.
authors.forename: Sunyang
authors.initials: S
authors.lastname: Fang
authors.affiliation: Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, 568 Zhongxingbei Road, Shaoxing 312000, China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D015996:Survival Rate
chemical_list: C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met
keywords: Apoptosis; Gastric carcinoma (GC); Growth; MET; MicroRNAs (miRNA); miR-449c