title: Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry.
abstract: Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17α-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development.
authors.affiliation: Cell Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP 11400 Montevideo, Uruguay.
authors.forename: Valentina
authors.initials: V
authors.lastname: Porro
authors.affiliation: Cell Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP 11400 Montevideo, Uruguay.
authors.forename: Romina
authors.initials: R
authors.lastname: Pagotto
authors.affiliation: Cell Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP 11400 Montevideo, Uruguay.
authors.forename: María Belén
authors.initials: MB
authors.lastname: Harreguy
authors.affiliation: Cell Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP 11400 Montevideo, Uruguay.
authors.forename: Sofía
authors.initials: S
authors.lastname: Ramírez
authors.affiliation: Transgenic and Experimental Animal Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP 11400 Montevideo, Uruguay.
authors.forename: Martina
authors.initials: M
authors.lastname: Crispo
authors.affiliation: Instituto de Salud y Ambiente del Litoral (ISAL), Ciudad Universitaria, Paraje El Pozo, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CP3000 Santa Fe, Argentina.
authors.forename: Clarisa
authors.initials: C
authors.lastname: Santamaría
authors.affiliation: Instituto de Salud y Ambiente del Litoral (ISAL), Ciudad Universitaria, Paraje El Pozo, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CP3000 Santa Fe, Argentina.
authors.forename: Enrique H
authors.initials: EH
authors.lastname: Luque
authors.affiliation: Instituto de Salud y Ambiente del Litoral (ISAL), Ciudad Universitaria, Paraje El Pozo, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CP3000 Santa Fe, Argentina. Electronic address: harodrig@fbcb.unl.edu.ar.
authors.forename: Horacio A
authors.initials: HA
authors.lastname: Rodríguez
authors.affiliation: Cell Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP 11400 Montevideo, Uruguay.
authors.forename: Mariela
authors.initials: M
authors.lastname: Bollati-Fogolín
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D004785:Environmental Pollutants; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D049452:Green Fluorescent Proteins; D004997:Ethinyl Estradiol
keywords: Estrogens; Flow cytometry; GFP; Male germ cells; Maturation; Oct4