title: Fit-for-Purpose Radio Receptor Assay for the Determination of Growth Hormone Secretagogues in Urine.
abstract: The everlasting pharmacological development is continuously producing new substances with potential doping abuse. Among these, secretagogues are very prone to misuse by athletes for their properties to release growth hormone (GH) and some limitations in the actual analytical methods to detect them. In this paper, an in-depth study on the key variables of the radio receptor method previously developed by our group is performed and a fit-for-purpose protocol is established. Thus, this sensitive and robust screening method is proposed as an intelligent and preventive antidoping method to detect new growth hormone secretagogues (GHSs) in exceptional suspicious urine samples obtained from athletes and will support the current detection methods based on liquid chromatography-mass spectrometry (LC-MS).
authors.affiliation: Bioanalysis Research Group, Neuroscience Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Ferro
authors.affiliation: Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, Barcelona, Spain Anapharm Biotech, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Gutiérrez-Gallego
authors.affiliation: Bioanalysis Research Group, Neuroscience Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bosch
authors.affiliation: Human Pharmacology and Neurosciences Research Group, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain Department of Pharmacology, Therapeutics and Toxicology, Universitat Autònoma de Barcelona-UAB, Cerdanyola del Vallés (Bellaterra), Spain.
authors.forename: M
authors.initials: M
authors.lastname: Farré
authors.affiliation: Bioanalysis Research Group, Neuroscience Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, Barcelona, Spain jsegura@imim.es.
authors.forename: J
authors.initials: J
authors.lastname: Segura
mesh_terms: D001667:Binding, Competitive; D002853:Chromatography, Liquid; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D054439:Ghrelin / Q000378:metabolism; D006023:Glycoproteins / Q000652:urine*; D013006:Growth Hormone / Q000652:urine*; D057809:HEK293 Cells; D006801:Humans; D013058:Mass Spectrometry; D058573:Performance-Enhancing Substances / Q000652:urine*; D054440:Receptors, Ghrelin / Q000378:metabolism
chemical_list: D054439:Ghrelin; D006023:Glycoproteins; C073416:MMS-68 protein, human; D058573:Performance-Enhancing Substances; D054440:Receptors, Ghrelin; D013006:Growth Hormone
keywords: GHS-R1a receptor; doping abuse; doping substances; growth hormone secretagogues; radio receptor assay