title: Enhancement of the antibiotic activity of aminoglycosides by extracts from Anadenanthera colubrine (Vell.) Brenan var. cebil against multi-drug resistant bacteria.
abstract: The aim of this work was to evaluate the antimicrobial activity of ethanol (EEAC) and hexane (HFAC) extracts from the stem bark of Anadenanthera colubrina (Vell.) Brenan var. cebil alone or in combination with aminoglycosides against multi-drug resistant (MDR) bacteria. Minimal inhibitory concentrations (MICs) of the extracts were determined by using microdilution assay. For the evaluation of extracts as modulators of antibiotic resistance, MICs of neomycin and amikacin were determined in presence or absence of each compound at sub-inhibitory concentrations. Both EEAC and HFAC did not show antimicrobial activity against MDR strains tested. However, the addition of EEAC and HFAC enhanced the activity of neomycin and amikacin against Staphylococcus aureus SA10 strain. When the natural products were replaced by chlorpromazine, the same effect was observed. Anadenanthera colubrine var. cebil may be a source of phytochemicals able to potentiate the aminoglycoside activity against MDR S. aureus by the inhibition of efflux pump.
authors.affiliation: a Laboratory of Research in Microbiology, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: Humberto M
authors.initials: HM
authors.lastname: Barreto
authors.affiliation: a Laboratory of Research in Microbiology, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: Kivia M R N
authors.initials: KM
authors.lastname: Coelho
authors.affiliation: a Laboratory of Research in Microbiology, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: Josie H L
authors.initials: JH
authors.lastname: Ferreira
authors.affiliation: b Laboratory of Clinical Microbiology, Federal University of Paraiba , João Pessoa , PB , Brazil.
authors.forename: Bernadete H C
authors.initials: BH
authors.lastname: Dos Santos
authors.affiliation: c Laboratory of Microbiology, Faculty of Higher Education of Floriano , Floriano , PI , Brazil.
authors.forename: Aislan P L
authors.initials: AP
authors.lastname: de Abreu
authors.affiliation: d Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri , Crato , CE , Brazil.
authors.forename: Henrique D M
authors.initials: HD
authors.lastname: Coutinho
authors.affiliation: e Laboratory of Natural Products, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: Romezio A C
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: e Laboratory of Natural Products, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: Taciana O
authors.initials: TO
authors.lastname: de Sousa
authors.affiliation: e Laboratory of Natural Products, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: Antonia M das G L
authors.initials: AM
authors.lastname: Citó
authors.affiliation: e Laboratory of Natural Products, Federal University of Piauí , Teresina , PI , Brazil.
authors.forename: José A D
authors.initials: JA
authors.lastname: Lopes
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects; D004357:Drug Synergism; D007887:Fabaceae / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009355:Neomycin / Q000494:pharmacology; D064209:Phytochemicals / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D064209:Phytochemicals; D010936:Plant Extracts; D000583:Amikacin; D009355:Neomycin
keywords: Anadenanthera colubrina var. cebil; Escherichia coli; Staphylococcus aureus; antimicrobial activity; chlorpromazine; efflux pump; modifying-resistance activity