title: A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration.
abstract: Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical factor, so we modified this method after examining multiple parameters (solvent, pH, buffers, and light exposure). The improved method is simple to implement, robust, accurate, and (most important) precise.
authors.affiliation: Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA. Electronic address: kevin.rupprecht@abbott.com.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Rupprecht
authors.affiliation: Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA.
authors.forename: Ewa Z
authors.initials: EZ
authors.lastname: Lang
authors.affiliation: Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA.
authors.forename: Svetoslava D
authors.initials: SD
authors.lastname: Gregory
authors.affiliation: Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Bergsma
authors.affiliation: Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA.
authors.forename: Tracey D
authors.initials: TD
authors.lastname: Rae
authors.affiliation: Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Fishpaugh
mesh_terms: D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D012997:Solvents; D012967:Sodium Dodecyl Sulfate
keywords: Concentration; Quantitative analysis; Sodium dodecyl sulfate (SDS)