title: Test-retest repeatability of myocardial blood flow and infarct size using ¹¹C-acetate micro-PET imaging in mice.
abstract: OBJECTIVE
  Global and regional responses of absolute myocardial blood flow index (iMBF) are used as surrogate markers to assess response to therapies in coronary artery disease. In this study, we assessed the test-retest repeatability of iMBF imaging, and the accuracy of infarct sizing in mice using (11)C-acetate PET.
  
  
  METHODS
  (11)C-Acetate cardiac PET images were acquired in healthy controls, endothelial nitric oxide synthase (eNOS) knockout transgenic mice, and mice after myocardial infarction (MI) to estimate global and regional iMBF, and myocardial infarct size compared to (18)F-FDG PET and ex-vivo histology results.
  
  
  RESULTS
  Global test-retest iMBF values had good coefficients of repeatability (CR) in healthy mice, eNOS knockout mice and normally perfused regions in MI mice (CR = 1.6, 2.0 and 1.5 mL/min/g, respectively). Infarct size measured on (11)C-acetate iMBF images was also repeatable (CR = 17 %) and showed a good correlation with the infarct sizes found on (18)F-FDG PET and histopathology (r (2) > 0.77; p < 0.05).
  
  
  CONCLUSIONS
  (11)C-Acetate micro-PET assessment of iMBF and infarct size is repeatable and suitable for serial investigation of coronary artery disease progression and therapy.
authors.affiliation: National Cardiac PET Centre, University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, Ontario, K1Y 4W7, Canada, Etienne.Croteau@USherbrooke.ca.
authors.forename: Etienne
authors.initials: E
authors.lastname: Croteau
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Renaud
authors.forename: Matthew
authors.initials: M
authors.lastname: McDonald
authors.forename: Ran
authors.initials: R
authors.lastname: Klein
authors.forename: Jean N
authors.initials: JN
authors.lastname: DaSilva
authors.forename: Rob S B
authors.initials: RS
authors.lastname: Beanlands
authors.forename: Robert A
authors.initials: RA
authors.lastname: deKemp
mesh_terms: D000085:Acetates / Q000493:pharmacokinetics*; D000818:Animals; D001783:Blood Flow Velocity; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D003326:Coronary Circulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology*; D055414:Myocardial Perfusion Imaging / Q000662:veterinary*; D049268:Positron-Emission Tomography / Q000662:veterinary*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals