title: Chemical profiling and anti-psoriatic activity of methanolic extract of Andrographis nallamalayana J.L.Ellis.
abstract: Andrographis nallamalayana is being widely used as tribal medicine in the treatment of leucoderma and mouth ulcers. Chemical profiling of methanolic extract of the whole plant (PE), using GC-MS and LC-MS, revealed the presence of compounds viz. α-tocopherol, β-sitosterol, tetradecanoic acid, monostearin, flavones/flavanones and their glycosides, chromones, etc. Topical application of imiquimod on the dorsal portion of male BALB/C mice resulted in the development of psoriatic symptoms (erythema, scaling, thickening and folding) with a mean disease activity index (DAI) of >7.0. Topical treatment with 100-μL PE (~6.4%/12.8%) formulations, for 12-days, resulted in the alleviation of disease symptoms. Compared to water-based formulations, emu oil-based formulation, PE400EO was found more effective in reducing the mean DAI (>84%), keratinocyte count (>65%) (p < 0.01) and interleukin-22 (~70%) (p < 0.05). We report, for the first time, anti-psoriatic activity of A. nallamalayana having great potential in developing a potent phytomedicine against psoriasis.
authors.affiliation: a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India.|b Department of Pharmacy and Biotechnology , University College of Technology, Osmania University , Hyderabad 500 007 , India.
authors.forename: Sunitha
authors.initials: S
authors.lastname: Parlapally
authors.affiliation: a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India.
authors.forename: Neeraja
authors.initials: N
authors.lastname: Cherukupalli
authors.affiliation: c National Centre for Mass Spectrometry , CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007 Telangana , India.|d Academy of Scientific and Innovative Research, CSIR-IICT , Hyderabad , India.
authors.forename: Sudarshana Reddy
authors.initials: SR
authors.lastname: Bhumireddy
authors.affiliation: c National Centre for Mass Spectrometry , CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007 Telangana , India.|d Academy of Scientific and Innovative Research, CSIR-IICT , Hyderabad , India.
authors.forename: Prabhakar
authors.initials: P
authors.lastname: Sripadi
authors.affiliation: b Department of Pharmacy and Biotechnology , University College of Technology, Osmania University , Hyderabad 500 007 , India.
authors.forename: Ravindernath
authors.initials: R
authors.lastname: Anisetti
authors.affiliation: a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India.
authors.forename: Charu Chandra
authors.initials: CC
authors.lastname: Giri
authors.affiliation: a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India.
authors.forename: Venkateswara Rao
authors.initials: VR
authors.lastname: Khareedu
authors.affiliation: a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India.
authors.forename: Dashavantha
authors.initials: D
authors.lastname: Reddy Vudem
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D044950:Flavanones / Q000032:analysis; D047309:Flavones / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000032:analysis; D007378:Interleukins / Q000378:metabolism; D008297:Male; D000432:Methanol; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000737:chemistry*; D011565:Psoriasis / Q000188:drug therapy*; D012855:Sitosterols / Q000032:analysis; D024502:alpha-Tocopherol / Q000032:analysis; D000097043:Interleukin-22
chemical_list: D044950:Flavanones; D047309:Flavones; D006027:Glycosides; D007378:Interleukins; D000432:Methanol; D010936:Plant Extracts; D012855:Sitosterols; D024502:alpha-Tocopherol; D000097043:Interleukin-22; C025473:gamma-sitosterol
keywords: Andrographis nallamalayana; antipsoriatic activity; chemical profiling; interleukin-22; mouse model