title: Label-free method for anti-glucopeptide antibody detection in Multiple Sclerosis.
abstract: Surface plasmon resonance technique is particularly interesting in immunology because it has the potential to visualize label-free antigen-antibody interactions in real-time, thus enabling antibody detection and monitoring. Herein we release the guidelines for the correct use of a method to detect specific antibodies directly in Multiple Sclerosis patients' sera using a glucopeptide-based label-free biosensor. The protocol describes the strategy employed for the immobilization of glucopeptide antigen onto a gold sensor chip and the evaluation of the specific binding of serum antibodies to the immobilized antigen. •Label-free method for the real time screening of disease-specific antibodies within a few minutes;•The described protocol employs small quantities of glucopeptide antigen and blood serum samples saving method-cost;•Stability of the immobilized glucopeptide antigen guarantees the regeneration of the surface allowing re-use the immunosensor with high automated throughput. The antibodies detected using the described methodology can be evaluated as biomarkers of Multiple Sclerosis. The SPR detection system is able to characterize antibodies significantly different from those evaluated in the classical enzyme-linked immunosorbent assays (ELISA).
authors.affiliation: Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology-PeptLab, Italy ; Department of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical Sciences and Nutraceutics, University of Florence, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Italy.
authors.forename: Feliciana
authors.initials: F
authors.lastname: Real-Fernández
authors.affiliation: Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology-PeptLab, Italy ; Department of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical Sciences and Nutraceutics, University of Florence, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Italy.
authors.forename: Giada
authors.initials: G
authors.lastname: Rossi
authors.affiliation: Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology-PeptLab, Italy ; Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Morgagni 50, I-50134 Firenze, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Lolli
authors.affiliation: Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology-PeptLab, Italy ; Department of Chemistry "Ugo Schiff", Via della Lastruccia 13, I-50019 Sesto Fiorentino, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Papini
authors.affiliation: Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology-PeptLab, Italy ; Department of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical Sciences and Nutraceutics, University of Florence, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Rovero
keywords: Antibody detection; Label-free immunoassay; Serodiagnosis; Surface plasmon resonance