title: Hemoglobin interacting proteins and implications of spectrin hemoglobin interaction.
abstract: In this report we have analyzed interacting partners of hemoglobin inside erythrocyte and sought possible implications of hemoglobin-spectrin interaction. Our list of identified cytosolic hemoglobin interacting proteins includes redox regulators like peroxiredoxin-2, Cu-Zn superoxide dismutase, catalase, aldehyde dehydrogenase-1, flavin reductase and chaperones like HSP70, α-hemoglobin stabilizing protein. Others include metabolic enzymes like carbonic anhydrase-1, selenium binding protein-1, purine nucleoside phosphorylase and nucleoside diphosphate kinase. Additionally, various membrane proteins like α and β spectrin, ankyrin, band3, protein4.1, actin and glyceraldehyde 3 phosphate dehydrogenase have been shown to interact with hemoglobin. Our result indicates that major membrane skeleton protein spectrin, that also has a chaperone like activity, helps to fold the unstable alpha-globin chains in vitro. Taken together our results could provide insight into a protein network evolved around hemoglobin molecule inside erythrocyte that may add a new perspective in understanding the hemoglobin function and homeostasis.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India.
authors.forename: Avik
authors.initials: A
authors.lastname: Basu
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India. Electronic address: abhijit.chakrabarti@saha.ac.in.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
mesh_terms: D000328:Adult; D001665:Binding Sites; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008297:Male; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D013049:Spectrin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D006454:Hemoglobins; D013049:Spectrin
keywords: Chaperone; Erythrocyte; Hemoglobin; Mass spectrometry; Protein–protein interaction; Spectrin