title: Expression of the tumor suppressor gene hypermethylated in cancer 1 in laryngeal carcinoma.
abstract: Hypermethylated in cancer 1 (HIC1) is a putative suppressor gene, cooperating with TP53 in the regulation of apoptosis. The promoter site of this gene contains CpG islands susceptible to methylation. Altered methylation leads to the silencing of HIC1. Persistent loss of HIC1 function reflects the attenuation of proapoptotic characteristics of TP53 and may constitute the background for carcinogenesis. Altered methylation profiles along with diminished expression of HIC1 were documented in a number of solid neoplasms. The aim of this study was to evaluate the expression of the HIC1 gene in laryngeal carcinoma. RNA was extracted from samples of laryngeal cancer and corresponding healthy tissues of 21 patients with advanced laryngeal cancer (T3-T4). The amount of RNA (cDNA) was evaluated using reverse transcription-quantitative polymerase chain reaction with GADPH as the reference gene. Data demonstrated that HIC1 expression was significantly reduced in laryngeal cancer tissues. The relative expression of HIC1 was found to be ~40% lower in tumor samples compared to that in healthy controls. The median tumor/normal tissue ratio for HIC1 was 0.615. These results suggest that low HIC1 expression may be associated with neoplastic transformation in the larynx.
authors.affiliation: Department of Laryngology, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-027, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Markowski
authors.affiliation: Department of Molecular Biology and Genetics, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-752, Poland ; CoE Research and Teaching of Molecular Biology of Matrix and Nanotechnology, Network of CoE BioMedTech Silesia, Katowice 40-752, Poland.
authors.forename: Aleksander L
authors.initials: AL
authors.lastname: Sieroń
authors.affiliation: Department of Laryngology, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-027, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kasperczyk
authors.affiliation: Department of Pathomorphology, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-752, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Ciupińska-Kajor
authors.affiliation: Department of Molecular Biology and Genetics, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-752, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Auguściak-Duma
authors.affiliation: Department of Human Anatomy, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-752, Poland.
authors.forename: Wirginia
authors.initials: W
authors.lastname: Likus
keywords: hypermethylated in cancer 1; laryngeal cancer; tumor suppressor gene