title: [Inhibition of antisense VEGF gene on the growth of glioma cells BT325].
abstract: OBJECTIVE
  To investigate the inhibitory effect of antisense VEGF gene on growth of human glioma BT325 and induced apoptosis in cells.
  
  
  METHODS
  The pcDNA3.1/anti-VEGF eukaryotic expression vector was constructed and transfected into human glioma BT325. The ELISA assay was used to assess the expression of VEGF gene. Soft agar colony formation, MTT assay and electron microscope were used to evaluate the proliferation, apoptosis and the morphological changes of BT325.
  
  
  RESULTS
  Compared with a control group, the level of VEGF expression was significantly decreased and was almost completely suppressed. The amount in soft agar colony at vector group and antisense VEGF gene group were 21 and 2, respectively. The growth of BT325 was significantly inhibited to 38.23% and resulted in the apoptotic morphology by antisense VEGF gene under electron microscope, compared to vector group.
  
  
  CONCLUSIONS
  Antisense VEGF gene inhibited the growth and proliferation, induced cell apoptosis, played an efficient role of anti-cancer in BT325 cells.
authors.forename: Licui
authors.initials: L
authors.lastname: Sun
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Lu
authors.forename: Weiyan
authors.initials: W
authors.lastname: Gong
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhenwu
authors.initials: Z
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016373:DNA, Antisense / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors; D005910:Glioma / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D006801:Humans; D016372:RNA, Antisense / Q000235:genetics* / Q000494:pharmacology; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D016373:DNA, Antisense; D016372:RNA, Antisense; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A