title: Comparing Wild American Grapes with Vitis vinifera: A Metabolomics Study of Grape Composition.
abstract: We analyzed via untargeted UHPLC-ESI-Q-TOF-MS the metabolome of the berry tissues (skin, pulp, seeds) of some American Vitis species (Vitis cinerea, Vitis californica, Vitis arizonica), together with four interspecific hybrids, and seven Vitis vinifera cultivars, aiming to find differences in the metabolomes of the American Vitis sp. versus Vitis vinifera. Apart from the known differences, that is, more complex content of anthocyanins and stilbenoids in the American grapes, we observed higher procyanidin accumulation (tens to hundreds of times) in the vinifera skin and seeds in comparison to American berries, and we confirmed this result via phloroglucinolysis. In the American grapes considered, we did not detect the accumulation of pleasing aroma precursors (terpenoids, glycosides), whereas they are common in vinifera grapes. We also found accumulation of hydrolyzable tannins and their precursors in the skin of the wild American grapes, which has never been reported earlier in any of the species under investigation. Such information is needed to improve the design of new breeding programs, lowering the risk of retaining undesirable characteristics in the chemical phenotype of the offspring.
authors.affiliation: †Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy.|§International Doctoral School in Bio-molecular Science, University of Trento, Via Sommarive 14, 38123 Povo-Trento, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Narduzzi
authors.affiliation: †Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy.
authors.forename: Jan
authors.initials: J
authors.lastname: Stanstrup
authors.affiliation: †Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Mattivi
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000378:metabolism; D005421:Flavoring Agents / Q000737:chemistry* / Q000378:metabolism; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D055432:Metabolomics; D027843:Vitis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D005421:Flavoring Agents
keywords: LC-MS; Vitis germplasm; hydrolyzable tannins identification; procyanidins quantification; untargeted comparative analysis