title: Antioxidant Potential and Toxicity Study of the Cerium Oxide Nanoparticles Synthesized by Microwave-Mediated Synthesis.
abstract: Monodispersed cerium oxide nanoparticle has been synthesized by microwave-mediated hydrothermal as well as microwave-mediated solvothermal synthesis. X-ray diffraction (XRD) data shows that the synthesized particles are single phase. SEM and TEM analysis suggest that particle synthesized by microwave-mediated solvothermal method are less agglomerated. In vitro toxicology study of the synthesized nanoceria particles has shown good free radical scavenging activity for NO and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assayed except superoxide radical within a concentration range of 25 to 75 ng ml(-1). Nanoceria particle also showed inhibition of Fe-ascorbate-induced lipid peroxidation (LPx) in chick liver mitochondrial fractions. Solvothermally synthesized nanoceria showed better protection against Fe-ascorbate-induced LPx than the hydrothermal one while the hydrothermally synthesized nanoceria showed better DPPH and NO scavenging activity. The ceria nanoparticles also prevented Fe-ascorbate-H2O2-induced carbonylation of bovine serum albumin in a dose-dependent manner. At higher concentration, i.e., 100 ng ml(-1), the synthesized nanoparticles showed a reverse trend in all the parameters measured indicating its toxicity at higher doses.
authors.affiliation: Department of Chemistry, Ravenshaw University, Cuttack, Odisha, India.
authors.forename: Siba
authors.initials: S
authors.lastname: Soren
authors.forename: Soumya Ranjan
authors.initials: SR
authors.lastname: Jena
authors.forename: Luna
authors.initials: L
authors.lastname: Samanta
authors.forename: Purnendu
authors.initials: P
authors.lastname: Parhi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D002563:Cerium / Q000633:toxicity*; D002645:Chickens; D003460:Crystallization; D016166:Free Radical Scavengers / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008872:Microwaves; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D009569:Nitric Oxide / Q000737:chemistry; D050050:Protein Carbonylation / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D012710:Serum Albumin, Bovine; D002563:Cerium; D009569:Nitric Oxide; C030583:ceric oxide