title: 17β-Estradiol transcriptionally modulates Nlrp1 and Nlrp3 inflammasomes in gonadectomized rats with inflammation.
abstract: BACKGROUND
  It has been reported that 17β-estradiol (E2) reduces the expression of inflammatory molecules, but there are no data that show the effect of E2 on the transcriptional regulation of innate immunity-related molecules and inflammasomes.
  
  
  OBJECTIVE
  To study the effect of 17β-estradiol (E2) on the transcriptional expression of the NLR family, pyrin domain containing 1 (Nlrp1) and (Nlrp3) inflammasomes, which are mediators of inflammation.
  
  
  METHODS
  Inflammation was induced in adult female gonadectomized (Gdx) rats by intramuscular injection of complete Freund's adjuvant (CFA). Measurements were taken at different times after the treatment. Gene expression determinations were done by quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  CFA-induced inflammation increased the transcription of Nlrp3, IL-1β (p < 0.05), vascular cell adhesion molecule 1 (VCAM1), E-selectin and estrogen receptor 1 alpha (ERα) (p < 0.001) and decreased the transcription of Nlrp1, Caspase-1, IL-33, NFKB1, ICAM1, ICAM2, GCRα, GCRβ, UCP3 and PGC1α (p < 0.001) compared to the control. The administration of E2 to the inflamed tissue significantly increased the expression of Nlrp1, NFKB1, ERα, UCP3, Caspase-1, E-selectin (p < 0.001), IL-18 and ERα (p < 0.05) and decreased IL-1β and VCAM1 (p < 0.005) compared to the control.
  
  
  CONCLUSIONS
  CFA differentially modulates the transcription of inflammasome-related genes and the administration of E2 increases the expression of ERα and Nlrp1 together with NFKB1, a key molecule in the activation of the inflammasomes. Finally, an analysis using the web interface GeneMANIA revealed an interaction between several genes, indicating a functional correlation in this model.
authors.affiliation: Laboratory of Molecular Biology .
authors.forename: Modesto
authors.initials: M
authors.lastname: Gómez López
authors.forename: Aarón
authors.initials: A
authors.lastname: Domínguez López
authors.forename: Edgar
authors.initials: E
authors.lastname: Abarca Rojano
authors.forename: Saúl
authors.initials: S
authors.lastname: Rojas Hernández
authors.forename: María de Los A
authors.initials: Mde L
authors.lastname: Martínez Godínez
authors.forename: Angel
authors.initials: A
authors.lastname: Miliar García
authors.forename: Rafael
authors.initials: R
authors.lastname: Campos Rodríguez
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004487:Edema / Q000139:chemically induced / Q000276:immunology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects*; D058847:Inflammasomes / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010052:Ovariectomy; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000276:Adjuvants, Immunologic; D002352:Carrier Proteins; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009419:Nerve Tissue Proteins; C526260:Nlrp1a protein, rat; C573346:Nlrp3 protein, rat; D004958:Estradiol; D005620:Freund's Adjuvant
keywords: Adjuvant; estrogens; gene expression; inflammasome; inflammation