title: An endophytic bacterium Acinetobacter calcoaceticus Sasm3-enhanced phytoremediation of nitrate-cadmium compound polluted soil by intercropping Sedum alfredii with oilseed rape.
abstract: Intensive agricultural system with high input of fertilizer results in high agricultural output. However, excessive fertilization in intensive agricultural system has great potential to cause nitrate and heavy metal accumulation in soil, which is adverse to human health. The main objective of the present study was to observe the effects of intercropping and inoculation of endophytic bacterium Acinetobacter calcoaceticus Sasm3 on phytoremediation of combined contaminated soil in oilseed rape (Brassica napus L.). The results showed that with Sasm3 inoculation, the biomass of rape was increased by 10-20% for shoot, 64% for root, and 23-29% for seeds while the nitrate accumulation in rape was decreased by 14% in root and by 12% in shoot. The cadmium concentration in rape increased significantly with mono-inoculating treatment, whereas it decreased significantly after intercropping treatment. By denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR analysis, the diversity of bacterial community and the number of nirS and nirK gene copies increased significantly with inoculation or/and intercropping treatment. In conclusion, the endophytic bacterium Sasm3-inoculated intercropping system not only improved the efficiency of clearing cadmium from soil without obstructing crop production, but also improved the quality of crop.
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Xu
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Pan
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Shengke
authors.initials: S
authors.lastname: Tian
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China. yfeng@zju.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Yang
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D029741:Brassica rapa / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D019187:Cadmium Compounds / Q000302:isolation & purification* / Q000378:metabolism; D000069599:Crop Production; D058645:Denaturing Gradient Gel Electrophoresis; D060026:Endophytes / Q000235:genetics / Q000378:metabolism; D058889:Molecular Typing; D009566:Nitrates / Q000302:isolation & purification* / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D031963:Sedum / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D019187:Cadmium Compounds; D009566:Nitrates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; C035196:cadmium nitrate
keywords: DGGE; Denitrification; Heavy metal; Quantitative PCR; Soil salinization