title: Paeoniflorin reduced BLP-induced inflammatory response by inhibiting the NF-κB signal transduction in pathway THP-1 cells.
abstract: Sepsis is a severe illness in which the bloodstream is overwhelmed by bacteria. Despite effective antibiotic treatment, the mortality of septic shock remains high. In this study, we examined a potential usage of paeoniflorin, anti-inflammatory component for the treatment of sepsis. We established an inflammatory cell line by stimulating human THP-1 cell line with bacterial lipoprotein (BLP), which resulted in an activation of nuclear factor κB (NF-κB) p65 dependent-signal pathway, and in consequence, an increase in tumor necrosis factor α (TNF-α) and interleukin (IL)-6 expression. With this model, we studied the effect of paeoniflorin on the expression of NF-κB and Toll-like receptor 2 (TLR2) mediated signal transduction. Our data indicated that paeoniflorin directly inhibited activation of NF-κB p65, thereby reduced the expression of TNF-α and IL-6 in the BLP stimulated THP-1 cells. Paeoniflorin was also found to inhibit IκB phosphorylation and degradation. However, no significant differences in TLR2 and myeloid differentiation factor 88 (MyD88) expression were observed; therefore, these signaling molecules may not have much anti-inflammatory effect in our cellular model. As such, our current study provided a molecular base for the potential use of paeoniflorin in therapeutic treatment of sepsis induced by bacterial lipoprotein.
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Bi
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Suming
authors.initials: S
authors.lastname: Zhou
keywords: TnF-α; Toll-like receptor; bacterial lipoprotein; paeoniflorin