title: The role of XRCC6/Ku70 in nasopharyngeal carcinoma.
abstract: The association between XRCC6/Ku70, an upstream player in the DNA double-strand break repair system, and the risk of nasopharyngeal carcinoma (NPC) was examined. In this case-control study, 176 NPC patients and 352 cancer-free controls were genotyped, and the associations of XRCC6 promoter T-991C (rs5751129), promoter G-57C (rs2267437), promoter G-31A (rs132770), and intron 3 (rs132774) polymorphisms with NPC risk were evaluated. NPC tissue samples were also assessed for their XRCC6 mRNA and protein expression by real-time quantitative reverse transcription PCR and Western blotting, respectively. With regard to the XRCC6 promoter T-991C, the TC and CC genotypes were associated with a significantly increased risk of NPC compared with wild-type TT genotype (adjusted odds ratio 2.02 and 3.42, 95% confidence interval 1.21-3.32 and 1.28-8.94, P=0.0072 and 0.0165, respectively). The mRNA and protein expression levels for NPC tissues revealed significantly lower XRCC6 mRNA and protein expression in the NPC samples with TC/CC genotypes compared to those with the TT genotype (P=0.0210 and 0.0164, respectively). These findings suggest that XRCC6 may play an important role in the carcinogenesis of NPC and could serve as a chemotherapeutic target for personalized medicine and therapy.
authors.affiliation: Graduate Institute of Medical Sciences, National Defence Medical Centre, Taipei, Taiwan, ROC; Taichung Armed Forces General Hospital, Taichung, Taiwan, ROC.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, ROC; Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC.
authors.forename: C-W
authors.initials: CW
authors.lastname: Tsai
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC.
authors.forename: C-M
authors.initials: CM
authors.lastname: Hsu
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC.
authors.forename: L-C
authors.initials: LC
authors.lastname: Shih
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC; Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, ROC.
authors.forename: W-S
authors.initials: WS
authors.lastname: Chang
authors.affiliation: Graduate Institute of Medical Sciences, National Defence Medical Centre, Taipei, Taiwan, ROC.
authors.forename: H-A
authors.initials: HA
authors.lastname: Shui
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, ROC; Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, ROC; Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, ROC. Electronic address: artbau2@gmail.com.
authors.forename: D-T
authors.initials: DT
authors.lastname: Bau
mesh_terms: D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000235:genetics*; D015153:Blotting, Western; D002277:Carcinoma; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D000072200:Ku Autoantigen; D008297:Male; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D011795:Surveys and Questionnaires; D013624:Taiwan
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen
keywords: XRCC6/Ku70; genotype; immunohistochemistry; nasopharyngeal carcinoma; non-homologous end-joining; polymorphism; real-time quantitative reverse transcription