title: Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among Mycobacterium tuberculosis Isolates in a High-HIV-Prevalence Setting.
abstract: In a head-to-head comparison of the MTBDRplus version 2.0 (Hain Lifescience), the Xpert MTB/RIF (Cepheid), and the Anyplex MTB/NTM (Seegene) assays, we demonstrated equal sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampin resistance with further analysis of discordances. The Xpert assay does not detect isoniazid resistance while the Anyplex assay showed high false positivity.
authors.affiliation: Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa strydom.kathy@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Strydom
authors.affiliation: Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa.
authors.forename: F
authors.initials: F
authors.lastname: Ismail
authors.affiliation: Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa.
authors.forename: M M Z
authors.initials: MM
authors.lastname: Matabane
authors.affiliation: National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa.
authors.forename: O
authors.initials: O
authors.lastname: Onwuegbuna
authors.affiliation: National Institute for Communicable Diseases: Centre for TB, Johannesburg, South Africa.
authors.forename: S V
authors.initials: SV
authors.lastname: Omar
authors.affiliation: Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Institute for Communicable Diseases: Centre for TB, Johannesburg, South Africa.
authors.forename: N
authors.initials: N
authors.lastname: Ismail
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D060005:Genotyping Techniques / Q000379:methods*; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin