title: Tuberculous pleurisy drives marked effector responses of γδ, CD4+, and CD8+ T cell subpopulations in humans.
abstract: Although tuberculous pleurisy (TP) presumably involves a hypersensitivity reaction, there is limited evidence indicating overreactive effector responses of γδ T cells and αβ T cells and their interrelation with Foxp3(+) Tregs in pleural and other compartments. We found that TP induced reciprocal representations of Foxp3(+) Tregs and Mtb phosphoantigen-specific Vγ2Vδ2 T cells in different anatomic compartments. Patients with TP exhibited appreciable numbers of "proliferating" Ki-67(+) Vγ2Vδ2 T cells in the airway where Foxp3(+) Tregs were not dominant, whereas striking increases in Foxp3(+) Tregs in the blood and pleural compartments coincided with low frequencies of Vγ2Vδ2 T cells. Interestingly, anti-tuberculosis chemotherapy control of Mtb infection in patients with TP reversed reciprocal representations of Foxp3(+) Tregs and proliferating Vγ2Vδ2 T cells. Surprisingly, despite high-level Foxp3(+) Tregs, TP appeared to drive overreactive responses of IFN-γ-producing Vγ2Vδ2, CD4(+)CD25(+), and CD8(+)CD25(+) T effector subpopulations, whereas IL-22-producing Vγ2Vδ2 T cells increased subtly. Th1 effector responses were sustained despite remarkable declines in Foxp3(+) Tregs at 1 mo after the treatment. Overreactive T effector responses of Mtb-reactive γδ T cells, αβ CD25(+)CD4(+), and CD25(+)CD8(+) T cell subpopulations appear to be immune features for TP. Increased Foxp3(+) Tregs might be responsive to overreactive TP but unable to influence T effector responses despite having an inverse relation with proliferating Vγ2Vδ2 T cells.
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Zeng
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Song
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Cai
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Su
authors.initials: S
authors.lastname: Huang
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Wandang
authors.initials: W
authors.lastname: Wang
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Xiang
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Dongzi
authors.initials: D
authors.lastname: Lin
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Ganbin
authors.initials: G
authors.lastname: Liu
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Junai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Crystal Y
authors.initials: CY
authors.lastname: Chen
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Shen
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Shen
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Lailong
authors.initials: L
authors.lastname: Yi
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA imxujunfa@163.com.
authors.forename: Junfa
authors.initials: J
authors.lastname: Xu
authors.affiliation: *Department of Clinical Immunology, Institute of Laboratory Medicine, and Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan China; Department of Respiration, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China; Department of Respiration, Affiliated Houjie Hospital of Guangdong Medical College, Dongguan, China; Dongguan Hospital for Prophylaxis and Treatment of Chronic Disease, Dongguan, China; Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.
authors.forename: Zheng W
authors.initials: ZW
authors.lastname: Chen
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology; D006801:Humans; D007378:Interleukins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010994:Pleura / Q000276:immunology* / Q000473:pathology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D014396:Tuberculosis, Pleural / Q000276:immunology* / Q000473:pathology; D000097043:Interleukin-22
chemical_list: D051858:Forkhead Transcription Factors; D007378:Interleukins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000097043:Interleukin-22; C418974:FOXP3 protein, human
keywords: Foxp3+ Treg; Mycobacterium tuberculosis; Th1; Th22; human Vγ2Vδ2 T cells