title: Propionic acid and butyric acid inhibit lipolysis and de novo lipogenesis and increase insulin-stimulated glucose uptake in primary rat adipocytes.
abstract: Fermentation of dietary fibers by colonic microbiota generates short-chain fatty acids (SCFAs), e.g., propionic acid and butyric acid, which have been described to have "anti-obesity properties" by ameliorating fasting glycaemia, body weight and insulin tolerance in animal models. In the present study, we therefore investigate if propionic acid and butyric acid have effects on lipolysis, de novo lipogenesis and glucose uptake in primary rat adipocytes. We show that both propionic acid and butyric acid inhibit isoproterenol- and adenosine deaminase-stimulated lipolysis as well as isoproterenol-stimulated lipolysis in the presence of a phosphodiesterase (PDE3) inhibitor. In addition, we show that propionic acid and butyric acid inhibit basal and insulin-stimulated de novo lipogenesis, which is associated with increased phosphorylation and thus inhibition of acetyl CoA carboxylase, a rate-limiting enzyme in fatty acid synthesis. Furthermore, we show that propionic acid and butyric acid increase insulin-stimulated glucose uptake. To conclude, our study shows that SCFAs have effects on fat storage and mobilization as well as glucose uptake in rat primary adipocytes. Thus, the SCFAs might contribute to healthier adipocytes and subsequently also to improved energy metabolism with for example less circulating free fatty acids, which is beneficial in the context of obesity and type 2 diabetes.
authors.affiliation: Department of Experimental Medical Science; Lund University ; Lund, Sweden.
authors.forename: Emilia
authors.initials: E
authors.lastname: Heimann
authors.affiliation: Department of Applied Nutrition and Food Chemistry; Lund University ; Lund, Sweden.
authors.forename: Margareta
authors.initials: M
authors.lastname: Nyman
authors.affiliation: Department of Experimental Medical Science; Lund University ; Lund, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Degerman
keywords: ACC, acetyl-CoA carboxylase; ADA, adenosine deaminase; AMPK, AMP-activated protein kinase; BA, butyric acid; BSA, bovine serum albumin; FFAR, free fatty acid receptor; GLUT, glucose transporter; GPCR, G-protein-coupled receptor; HSL, hormone-sensitive lipase; ISO, isoproterenol; KRBH, Krebs-Ringer bicarbonate-HEPES; KRH, Krebs Ringer-HEPES; PA, propionic acid; PDE, cyclic nucleotide phosphodiesterase; SCFAs, short-chain fatty acids; T2D, type 2 diabetes; adipocyte; metabolism; obesity; short-chain fatty acid; type 2 diabetes