title: Mycobacterium tuberculosis Rv1265 promotes mycobacterial intracellular survival and alters cytokine profile of the infected macrophage.
abstract: Mycobacterium tuberculosis cAMP and underlying regulatory network are crucial for its survival and thrive in the presence of numerous stresses mounted by the host. Our studies mainly focus on the cAMP-induced M. tuberculosis gene Rv1265, which was shown to be up-regulated under hypoxia and during macrophage infection by addition of exogenous cAMP. To explore the role of Rv1265 in host-pathogen interactions, Rv1265 was expressed in a non-pathogenic Mycobacterium smegmatis. We found that Rv1265 was associated with cell envelope and can up-regulate some cell wall fatty acid components, especially the C26:0. The survival of the recombinant Ms_Rv1265 was enhanced within macrophages and under stress conditions such as low pH and SDS. Macrophages infected with Ms_Rv1265 increased transcription of pro-inflammatory cytokines IL-1β, IL-6, and IL-12 P40 and anti-inflammatory cytokine IL-10 possibly through activation of NF-κB and ERK1/2 pathway. Our findings indicate that Rv1265 can enhance mycobacterial survival within macrophages, and perturb the cytokine profile of macrophage.
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Luo
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zeng
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Minqiang
authors.initials: M
authors.lastname: Liu
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.|b Department of Clinical Microbiology , College of Medical Laboratory Sciences, Omdurman Islamic University , Omdurman , Khartoum , Sudan.
authors.forename: Abualgasim Elgaili
authors.initials: AE
authors.lastname: Abdalla
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Longxiang
authors.initials: L
authors.lastname: Xie
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Institute of Modern Biopharmaceuticals , Southwest University , Chongqing 400715 , China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology*; D050296:Microbial Viability; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D015398:Signal Transduction; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D005227:Fatty Acids; D016328:NF-kappa B
keywords: Mycobacterium tuberculosis; Rv1265; cytokines; fatty acids; macrophage