title: Bicarbonate supplementation enhanced biofuel production potential as well as nutritional stress mitigation in the microalgae Scenedesmus sp. CCNM 1077.
abstract: The aim of the present study was to find out the optimum sodium bicarbonate concentration to produce higher biomass with higher lipid and carbohydrate contents in microalgae Scenedesmus sp. CCNM 1077. The role of bicarbonate supplementation under different nutritional starvation conditions was also evaluated. The results clearly indicate that 0.6 g/L sodium bicarbonate was optimum concentration resulting in 20.91% total lipid and 25.56% carbohydrate along with 23% increase in biomass production compared to normal growth condition. Addition of sodium bicarbonate increased the activity of nutrient assimilatory enzymes, biomass, lipid and carbohydrate contents under different nutritional starvation conditions. Nitrogen starvation with bicarbonate supplementation resulted in 54.03% carbohydrate and 34.44% total lipid content in microalgae Scenedesmus sp. CCNM 1077. These findings show application of bicarbonate grown microalgae Scenedesmus sp. CCNM 1077 as a promising feedstock for biodiesel and bioethanol production.
authors.affiliation: Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Pancha
authors.affiliation: Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Kaumeel
authors.initials: K
authors.lastname: Chokshi
authors.affiliation: Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Tonmoy
authors.initials: T
authors.lastname: Ghosh
authors.affiliation: Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Chetan
authors.initials: C
authors.lastname: Paliwal
authors.affiliation: Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Rahulkumar
authors.initials: R
authors.lastname: Maurya
authors.affiliation: Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D056804:Biofuels; D018533:Biomass; D008055:Lipids / Q000032:analysis; D058086:Microalgae / Q000187:drug effects / Q000502:physiology*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000172:deficiency*; D010758:Phosphorus / Q000172:deficiency*; D010860:Pigments, Biological / Q000032:analysis; D044444:Scenedesmus / Q000187:drug effects / Q000502:physiology*; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D056804:Biofuels; D008055:Lipids; D009566:Nitrates; D010860:Pigments, Biological; D010758:Phosphorus; D017693:Sodium Bicarbonate; D009584:Nitrogen
keywords: Carbohydrate; Microalgae; Neutral lipid; Nutritional starvation; Sodium bicarbonate