title: Antimicrobial susceptibilities and random amplified polymorphic DNA-PCR fingerprint characterization of Lactococcus lactis ssp. lactis and Lactococcus garvieae isolated from bovine intramammary infections.
abstract: In total, 181 streptococci-like bacteria isolated from intramammary infections (IMI) were submitted by a veterinary clinic to Quality Milk Production Services (QMPS, Cornell University, Ithaca, NY). The isolates were characterized by sequence analysis, and 46 Lactococcus lactis ssp. lactis and 47 Lactococcus garvieae were tested for susceptibility to 17 antibiotics. No resistant strains were found for β-lactam antibiotics widely used in clinical practice (penicillin, ampicillin, and amoxicillin), and all minimum inhibitory concentrations (MIC) were far from the resistance breakpoints. Eight strains had MIC intermediate to cefazolin. The random amplification of polymorphic DNA (RAPD)-PCR fingerprint patterns showed a slightly higher heterogeneity for Lc. lactis ssp. lactis isolates than for Lc. garvieae isolates.
authors.affiliation: Food Biotechnology, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, PO Box 1627, FI-70210 Kuopio, Finland.
authors.forename: C
authors.initials: C
authors.lastname: Plumed-Ferrer
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Venezie, Sez. terr. Vicenza viale Fiume 78, 36100 Vicenza, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Barberio
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853.
authors.forename: R
authors.initials: R
authors.lastname: Franklin-Guild
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853.
authors.forename: B
authors.initials: B
authors.lastname: Werner
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853.
authors.forename: P
authors.initials: P
authors.lastname: McDonough
authors.affiliation: Northern Valley Dairy Production Medicine Center, 900 N Wabasha, Plainview, MN 55964.
authors.forename: J
authors.initials: J
authors.lastname: Bennett
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853.
authors.forename: G
authors.initials: G
authors.lastname: Gioia
authors.affiliation: Università degli Studi di Milano, Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Via Celoria 10, 20133 Milan, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Rota
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853.
authors.forename: F
authors.initials: F
authors.lastname: Welcome
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853.
authors.forename: D V
authors.initials: DV
authors.lastname: Nydam
authors.affiliation: Cornell University, Animal Health Diagnostic Center, 240 Farrier Road, Ithaca, NY 14853; Università degli Studi di Milano, Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Via Celoria 10, 20133 Milan, Italy. Electronic address: pm389@cornell.edu.
authors.forename: P
authors.initials: P
authors.lastname: Moroni
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology; D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002437:Cefazolin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D017045:Lactococcus / Q000187:drug effects / Q000302:isolation & purification*; D013294:Lactococcus lactis / Q000187:drug effects / Q000302:isolation & purification*; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D000667:Ampicillin; D000658:Amoxicillin; D002437:Cefazolin
keywords: Lactococcus garvieae; Lactococcus lactis ssp. lactis; intramammary infection; minimum inhibitory concentration; random amplification of polymorphic DNA (RAPD)-PCR fingerprint