title: Dietary Fiber Intake is Associated with Increased Colonic Mucosal GPR43+ Polymorphonuclear Infiltration in Active Crohn's Disease.
abstract: G protein-coupled receptor 43/free fatty acid receptor 2 (GPR43/FFAR2) is essential for polymorphonuclear (PMN) recruitment. We investigated the expression of GPR43/FFAR2 in the colon from Crohn's disease patients and whether dietary fiber in enteral nutrition increases GPR43+ polymorphonuclear infiltration in mucosa. Segments of ascending colon and white blood cells from peripheral blood were obtained from 46 Crohn's disease patients and 10 colon cancer patients. The Crohn's disease patients were grouped by the activity of disease (active or remission) and enteral nutrition with or without dietary fiber. Histological feature, expression and location of GPR43/FFAR2 and level of tumor necrosis factor-α (TNF-α), interleukine-6 (IL-6) and myeloperoxidase were assessed. The results of hematoxylin-eosin and immunohistochemistry staining revealed that the infiltration of immune cells, including GPR43+ PMN, was more severe in active Crohn's disease patients who consumed normal food or enteral nutrition with dietary fiber than in remission patients and colon cancer patients. This finding was supported by the results of GPR43 and myeloperoxidase expression. Active Crohn's disease (CD) patients who consumed enteral nutrition without dietary fiber exhibited severe immune cell infiltration similar to the other active CD patients, but GPR43+ PMNs were rarely observed. The level of TNF-α mRNA in active Crohn's disease patients was higher than those of the other patients. In conclusion, the use of dietary fiber in enteral nutrition by active Crohn's disease patients might increase GPR43+ PMNs infiltration in colon mucosa. This effect was not observed in Crohn's disease patients in remission.
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. zhao_ming_li@163.com.|Department of General Surgery, Nanfang Hospital, South Medical University, No. 1838 North Guangzhoudadao Rd., Guangzhou 510515, China. zhao_ming_li@163.com.
authors.forename: Mingli
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. dr_zhuweiming@126.com.
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. gongjianfeng@aliyun.com.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. zuolugen@126.com.
authors.forename: Lugen
authors.initials: L
authors.lastname: Zuo
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. zhj880616@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. jingdianjing99@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. liningnanjingjqzy@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of General Surgery, Jinling Hospital, No. 305 East Zhongshan Rd., Nanjing 210002, China. lijieshou@163.com.
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D044682:Colon, Ascending / Q000276:immunology / Q000378:metabolism; D003110:Colonic Neoplasms / Q000097:blood / Q000178:diet therapy / Q000276:immunology; D003424:Crohn Disease / Q000097:blood / Q000178:diet therapy / Q000276:immunology*; D004043:Dietary Fiber / Q000008:administration & dosage*; D004750:Enteral Nutrition / Q000379:methods; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D012074:Remission Induction; D012189:Retrospective Studies; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled
chemical_list: D004043:Dietary Fiber; D015850:Interleukin-6; D009195:Peroxidase; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; C473103:FFAR2 protein, human; D043562:Receptors, G-Protein-Coupled; C508600:IL6 protein, human; C439524:TNF protein, human
keywords: Crohn’s disease; GPR43/FFAR2; dietary fibre; polymorphonuclear