title: Chondrogenic capability of osteoarthritic chondrocytes from the trapeziometacarpal and hip joints.
abstract: Osteoarthritis is the most common degenerative disease of joints like the hip and the trapeziometacarpal joint (rhizarthrosis). In this in vitro study, we compared the chondrogenesis of chondrocytes derived from the trapezium and the femoral head cartilage of osteoarthritic patients to have a deeper insight on trapezium chondrocyte behavior as autologous cell source for the repair of cartilage lesions in rhizarthrosis. Chondrocytes collected from trapezium and femoral head articular cartilage were cultured in pellets and analyzed for chondrogenic differentiation, cell proliferation, glycosaminoglycan production, gene expression of chondrogenic and fibrous markers, histological and immunohistochemical analyses. Our results showed a higher cartilaginous matrix deposition and a lower fibrocartilaginous phenotype of the femoral chondrocytes with respect to the trapezium chondrocytes assessed by a higher absolute glycosaminoglycan and type II collagen production, thus demonstrating a superior chondrogenic potential of the femoral with respect to the trapezium chondrocytes. The differences in chondrogenic potential between trapezium and femoral head chondrocytes confirmed a lower regenerative capability in the trapezium than in the femoral head cartilage due to the different environment and loading acting on these joints that affects the metabolism of the resident cells. This could represent a limitation to apply the cell therapy for rhizoarthrosis.
authors.affiliation: Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy.
authors.forename: Arianna B
authors.initials: AB
authors.lastname: Lovati
authors.affiliation: Laboratory of Experimental Biochemistry and Molecular Biology, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Colombini
authors.affiliation: MAP Lab, Filarete Foundation, Milan, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Recordati
authors.affiliation: Laboratory of Experimental Biochemistry and Molecular Biology, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ceriani
authors.affiliation: Hip Department, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Zagra
authors.affiliation: Hand Surgery Unit, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Berzero
authors.affiliation: Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy. matteo.moretti@grupposandonato.it.
authors.forename: Matteo
authors.initials: M
authors.lastname: Moretti
mesh_terms: D000368:Aged; D052737:Carpometacarpal Joints / Q000000981:diagnostic imaging / Q000473:pathology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000473:pathology*; D020219:Chondrogenesis; D005270:Femur Head / Q000473:pathology; D005786:Gene Expression Regulation; D006621:Hip Joint / Q000000981:diagnostic imaging / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000473:pathology*
keywords: Cellular behaviour; Chondrocytes; Differentiation; Osteoarthritis; Trapezium