title: Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform.
abstract: Herein, for the first time, we presented a simple and general approach by using personal glucose meters (PGM) for portable and ultrasensitive detection of microbial pathogens. Upon addition of pathogenic bacteria, glucoamylase-quaternized magnetic nanoparticles (GA-QMNPS) conjugates were disrupted by the competitive multivalent interactions between bacteria and QMNPS, resulting in the release of GA. After magnetic separation, the free GA could catalyze the hydrolysis of amylose into glucose for quantitative readout by PGM. In such way, PGM was transmuted into a bacterial detection device and extremely low detection limits down to 20 cells mL(-1) was achieved. More importantly, QMNPS could inhibit the growth of the bacteria and destroy its cellular structure, which enabled bacteria detection and inhibition simultaneously. The simplicity, portability, sensitivity and low cost of presented work make it attractive for clinical applications.
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Gao
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D001431:Bacteriological Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D015190:Blood Glucose Self-Monitoring / Q000191:economics / Q000295:instrumentation*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000648:ultrastructure; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D050296:Microbial Viability; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D058185:Magnetite Nanoparticles
keywords: antibacterial agents; glucoamylase; nanoparticles; pathogen detection; personal glucose meters