title: Detection of an inversion in the Ty-2 region between S. lycopersicum and S. habrochaites by a combination of de novo genome assembly and BAC cloning.
abstract: A chromosomal inversion associated with the tomato Ty - 2 gene for TYLCV resistance is the cause of severe suppression of recombination in a tomato Ty - 2 introgression line. Among tomato and its wild relatives inversions are often observed, which result in suppression of recombination. Such inversions hamper the transfer of important traits from a related species to the crop by introgression breeding. Suppression of recombination was reported for the TYLCV resistance gene, Ty-2, which has been introgressed in cultivated tomato (Solanum lycopersicum) from the wild relative S. habrochaites accession B6013. Ty-2 was mapped to a 300-kb region on the long arm of chromosome 11. The suppression of recombination in the Ty-2 region could be caused by chromosomal rearrangements in S. habrochaites compared with S. lycopersicum. With the aim of visualizing the genome structure of the Ty-2 region, we compared the draft de novo assembly of S. habrochaites accession LYC4 with the sequence of cultivated tomato ('Heinz'). Furthermore, using populations derived from intraspecific crosses of S. habrochaites accessions, the order of markers in the Ty-2 region was studied. Results showed the presence of an inversion of approximately 200 kb in the Ty-2 region when comparing S. lycopersicum and S. habrochaites. By sequencing a BAC clone from the Ty-2 introgression line, one inversion breakpoint was identified. Finally, the obtained results are discussed with respect to introgression breeding and the importance of a priori de novo sequencing of the species involved.
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University & Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Anne-Marie A
authors.initials: AM
authors.lastname: Wolters
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University & Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Myluska
authors.initials: M
authors.lastname: Caro
authors.affiliation: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancunnandajie 12, Beijing, 100081, People's Republic of China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Dong
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University & Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Richard
authors.initials: R
authors.lastname: Finkers
authors.affiliation: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancunnandajie 12, Beijing, 100081, People's Republic of China.
authors.forename: Jianchang
authors.initials: J
authors.lastname: Gao
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University & Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.affiliation: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancunnandajie 12, Beijing, 100081, People's Republic of China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancunnandajie 12, Beijing, 100081, People's Republic of China.
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Du
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University & Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands. bai.yuling@wur.nl.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Bai
mesh_terms: D007446:Chromosome Inversion; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers; D018551:Solanum lycopersicum / Q000235:genetics* / Q000821:virology; D009029:Mosaic Viruses; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D032322:Solanum / Q000235:genetics* / Q000821:virology
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers