title: Anti-fatigue activity of a novel polysaccharide conjugates from Ziyang green tea.
abstract: The aim of this study was to investigate the anti-fatigue activity of polysaccharides from Ziyang green tea. Polysaccharides were isolated from Ziyang green tea and its physicochemical properties were analyzed. Meanwhile, a 4-week weight-loaded swimming test of mice was established and polysaccharides were orally administrated during exercise. The biochemical parameters related to fatigue were determined, such as exhaustive time, blood urea nitrogen (BUN), blood lactate acid (Bla) levels and lactic dehydrogenase (LDH) activity in serum, Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px) activities, Malondialdehyde (MDA) and glycogen levels in skeletal muscle. The results demonstrated that polysaccharide from Ziyang green tea was a selenium-polysaccharide-protein conjugate (Se-TP), and Se-TP administration significantly prolonged exhaustive time and increased glycogen level and GSH-Px activity in muscle, in addition, markedly decreased BUN, Bla levels and LDH activity in serum and MDA level in muscle. In conclusion, Se-TP treatment can significantly improve exercise-induced fatigue and decrease the oxidative stress induced by the exhaustive exercise.
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China. Electronic address: chimu@snnu.edu.cn.
authors.forename: Aiping
authors.initials: A
authors.lastname: Chi
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Chenzhe
authors.initials: C
authors.lastname: Kang
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005221:Fatigue / Q000188:drug therapy / Q000209:etiology; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D010805:Physical Conditioning, Animal; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D013662:Tea
keywords: Exercise-induced fatigue; Polysaccharide; Selenium