title: Reaction analysis on Yb(3+) and DNA based on quantum dots: The design of a fluorescent reversible off-on mode.
abstract: Even though various strategies have reported for DNA detection, development of a simple, time-saving and specific fluorescent sensing platform still remains a desired goal. In this work, a quantum dots (QDs) based fluorescent reversible "off-on" mode was developed for sensitively recognition of herring sperm DNA (hsDNA). Firstly, in the "turn off" stage, the fluorescence of glutathione (GSH) capped CdTe QDs could be effectively quenched by ytterbium ion (Yb(3+)) was due to the occurrence of the electron transfer between Yb(3+) and the photoexcited QDs. And then, in the following "turn on" stage, with the effective binding reaction of Yb(3+) to hsDNA, the fluorescence intensity of GSH-capped CdTe QDs enhanced. Under the optimal conditions, the linear range of fluorescence versus the concentration of hsDNA was 0.010-12 μg/mL, and the detection limit was 3.033 ng/mL. In addition, the reaction mechanism among GSH-capped CdTe QDs, Yb(3+) and hsDNA were investigated by fluorescence spectroscopy, UV-vis spectrophotometry, fluorescence lifetime measurement and viscosity measurements. This analytical fluorescent reversible "off-on" pattern offered a way with good sensitivity and selectivity for the detection of hsDNA.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Shaopu
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Chenxia
authors.initials: C
authors.lastname: Hao
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Nianxi
authors.initials: N
authors.lastname: Kuang
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: heyq@swu.edu.cn.
authors.forename: Youqiu
authors.initials: Y
authors.lastname: He
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D008297:Male; D008968:Molecular Conformation; D010316:Particle Size; D045663:Quantum Dots; D013094:Spermatozoa / Q000737:chemistry; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*; D015018:Ytterbium / Q000032:analysis* / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D004247:DNA; D015018:Ytterbium; D013691:Tellurium; C028337:cadmium telluride
keywords: Herring sperm DNA; Intercalative binding; Off–on mode; Quantum dots; Ytterbium ion