title: Bactericidal Effect of Photolysis of H2O2 in Combination with Sonolysis of Water via Hydroxyl Radical Generation.
abstract: The bactericidal effect of hydroxyl radical (·OH) generated by combination of photolysis of hydrogen peroxide (H2O2) and sonolysis of water was examined under the condition in which the yield of ·OH increased additively when H2O2 aqueous solution was concomitantly irradiated with laser and ultrasound. The suspension of Staphylococcus aureus mixed with the different concentrations of H2O2 was irradiated simultaneously with a laser light (wavelength: 405 nm, irradiance: 46 and 91 mW/cm2) and ultrasound (power: 30 w, frequency: 1.65 MHz) at 20 ± 1°C of the water bulk temperature for 2 min. The combination of laser and ultrasound irradiation significantly reduced the viable bacterial count in comparison with the laser irradiation of H2O2 alone. By contrast, the ultrasound irradiation alone exerted almost no bactericidal effect. These results suggested that the combination effect of photolysis of H2O2 and sonolysis of water on bactericidal activity was synergistic. A multi-way analysis of variance also revealed that the interaction of H2O2 concentration, laser power and ultrasound irradiation significantly affected the bactericidal activity. Since the result of oxidative DNA damage evaluation demonstrated that the combination of laser and ultrasound irradiation significantly induced oxidative damage of bacterial DNA in comparison with the laser irradiation of H2O2 alone, it was suggested that the combination effect of photolysis of H2O2 and sonolysis of water on bactericidal activity would be exerted via oxidative damage of cellular components such as DNA.
authors.affiliation: Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan.
authors.forename: Hong
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Laboratory for Redox Regulation, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Kanno
authors.affiliation: Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Laboratory for Redox Regulation, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Niwano
mesh_terms: D004249:DNA Damage; D004269:DNA, Bacterial / Q000378:metabolism*; D006861:Hydrogen Peroxide; D007834:Lasers; D010084:Oxidation-Reduction; D010782:Photolysis; D013211:Staphylococcus aureus / Q000378:metabolism*; D000069453:Ultrasonic Waves; D014867:Water / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D014867:Water; D006861:Hydrogen Peroxide