title: The Role of α-CTD in the Genome-Wide Transcriptional Regulation of the Bacillus subtilis Cells.
abstract: The amino acid sequence of the RNA polymerase (RNAP) α-subunit is well conserved throughout the Eubacteria. Its C-terminal domain (α-CTD) is important for the transcriptional regulation of specific promoters in both Escherichia coli and Bacillus subtilis, through interactions with transcription factors and/or a DNA element called the "UP element". However, there is only limited information regarding the α-CTD regulated genes in B. subtilis and the importance of this subunit in the transcriptional regulation of B. subtilis. Here, we established strains and the growth conditions in which the α-subunit of RNAP was replaced with a C-terminally truncated version. Transcriptomic and ChAP-chip analyses revealed that α-CTD deficiency reduced the transcription and RNAP binding of genes related to the utilization of secondary carbon sources, transition state responses, and ribosome synthesis. In E. coli, it is known that α-CTD also contributes to the expression of genes related to the utilization of secondary carbon sources and ribosome synthesis. Our results suggest that the biological importance of α-CTD is conserved in B. subtilis and E. coli, but that its specific roles have diversified between these two bacteria.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Satohiko
authors.initials: S
authors.lastname: Murayama
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Onuma
authors.initials: O
authors.lastname: Chumsakul
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Naotake
authors.initials: N
authors.lastname: Ogasawara
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Oshima
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D002244:Carbon / Q000494:pharmacology; D047369:Chromatin Immunoprecipitation; D004269:DNA, Bacterial / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D056426:Genetic Loci; D016680:Genome, Bacterial; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012808:Sigma Factor / Q000378:metabolism; D061805:Transcription Elongation, Genetic / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D014994:Xylose / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D046912:Multiprotein Complexes; D012808:Sigma Factor; D007544:Isopropyl Thiogalactoside; D002244:Carbon; D014994:Xylose; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit