title: Diversification of memory B cells drives the continuous adaptation of secretory antibodies to gut microbiota.
abstract: Secretory immunoglobulin A (SIgA) shields the gut epithelium from luminal antigens and contributes to host-microbe symbiosis. However, how antibody responses are regulated to achieve sustained host-microbe interactions is unknown. We found that mice and humans exhibited longitudinal persistence of clonally related B cells in the IgA repertoire despite major changes in the microbiota during antibiotic treatment or infection. Memory B cells recirculated between inductive compartments and were clonally related to plasma cells in gut and mammary glands. Our findings suggest that continuous diversification of memory B cells constitutes a central process for establishing symbiotic host-microbe interactions and offer an explanation of how maternal antibodies are optimized throughout life to protect the newborn.
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lindner
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Irene
authors.initials: I
authors.lastname: Thomsen
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wahl
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Milas
authors.initials: M
authors.lastname: Ugur
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Maya K
authors.initials: MK
authors.lastname: Sethi
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Friedrichsen
authors.affiliation: Laboratory for Animal Science, Hannover Medical School, Hannover, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Smoczek
authors.affiliation: Department for Internal Medicine I, University Hospital Schleswig Holstein, Kiel, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Ott
authors.affiliation: Pediatric Gastroenterology, Hannover Medical School, Hannover, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Baumann
authors.affiliation: Institute of Medical Microbiology and Hospital Epidemiology, Hannover Medical School, Hannover, Germany.
authors.forename: Sebastian
authors.identifier: 0000000195837505
authors.initials: S
authors.lastname: Suerbaum
authors.affiliation: 1] Department for Internal Medicine I, University Hospital Schleswig Holstein, Kiel, Germany. [2] Institute of Clinical Molecular Biology, University Hospital Schleswig-Holstein, Kiel, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schreiber
authors.affiliation: Laboratory for Animal Science, Hannover Medical School, Hannover, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Bleich
authors.affiliation: INSERM UMR1163, Laboratory of Intestinal Immunity, Institut Imagine, Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gaboriau-Routhiau
authors.affiliation: INSERM UMR1163, Laboratory of Intestinal Immunity, Institut Imagine, Paris, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Cerf-Bensussan
authors.affiliation: The Mina &Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Helena
authors.initials: H
authors.lastname: Hazanov
authors.affiliation: The Mina &Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Ramit
authors.initials: R
authors.lastname: Mehr
authors.affiliation: Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, Oslo, Norway.
authors.forename: Preben
authors.initials: P
authors.lastname: Boysen
authors.affiliation: Institute of Clinical Molecular Biology, University Hospital Schleswig-Holstein, Kiel, Germany.
authors.forename: Philip
authors.initials: P
authors.lastname: Rosenstiel
authors.affiliation: 1] Institute of Immunology, Hannover Medical School, Hannover, Germany. [2] Institute of Molecular Medicine, RWTH Aachen, Aachen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Pabst
mesh_terms: D000222:Adaptation, Physiological / Q000276:immunology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000906:Antibodies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D054884:Host-Pathogen Interactions / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology; D008321:Mammary Glands, Animal / Q000276:immunology / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D064307:Microbiota / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D009154:Mutation; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013559:Symbiosis / Q000187:drug effects / Q000276:immunology; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D012336:RNA, Ribosomal, 16S