title: Cloning and characterization of a novel PE_PGRS60 protein (Rv3652) of Mycobacterium tuberculosis H37 Rv exhibit fibronectin-binding property.
abstract: The binding of pathogenic bacteria to extracellular matrix components enhances adhesion and invasion of host cells. The host receptor proteins such as fibronectin (Fn) targeted to pathogenic ligands that have clinical importance. In the present study, we cloned, expressed, purified, and identified a novel Fn-binding protein from PE_PGRS60 (Rv3652) of Mycobacterium tuberculosis H37 Rv. The protein product of Rv3652 showed optimum binding efficiency to 10 ng Fn at 0.2 µg purified protein of PE_PGRS60 and 20 ng Fn at 0.2 µg concentrations, respectively. PE_PGRS60 protein (primary sequences) of different pathogenic mycobacterium species retrieved from NCBI exhibited complete homology at the 104 residues on multiple sequence alignment. The primary sequence of protein from H37 Rv was further used to predict cleavage signals. The secondary structure prediction method revealed a number of residues responsible for alpha helices formation and percentage of residues participating in the random coils and extended strands. In addition, online prediction tools such as B- and T-cell epitopes showed the surface probability scale and antigenic propensity scale. The current finding opens new opportunity to mycobacterial survival and pathogenesis research of PE-polymorphic GC-rich repetitive sequences (PE-PGRS) family proteins.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Delhi, India.
authors.forename: Laxman S
authors.initials: LS
authors.lastname: Meena
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Delhi, India.
authors.forename: Jaishree
authors.initials: J
authors.lastname: Meena
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005353:Fibronectins / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011485:Protein Binding; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D005353:Fibronectins; D021382:Protein Sorting Signals
keywords: B- and T-cell epitopes; Mycobacterium tuberculosis; PE-PGRS; fibronectin-binding protein; host bacterial interaction; pathogenesis