title: Polyunsaturated fatty acids influence differential biosynthesis of oxylipids and other lipid mediators during bovine coliform mastitis.
abstract: Coliform mastitis is a severe and sometimes fatal disease characterized by an unregulated inflammatory response. The initiation, progression, and resolution of inflammatory responses are regulated, in part, by potent oxylipid metabolites derived from polyunsaturated fatty acids. The purpose of this study was to characterize the biosynthesis and diversity of oxylipid metabolites during acute bovine coliform mastitis. Eleven cows diagnosed with naturally occurring acute systemic coliform mastitis and 13 healthy control cows, matched for lactation number and days in milk, were selected for comparison of oxylipid and free fatty acid concentrations in both milk and plasma. Oxylipids and free fatty acids were quantified using liquid chromatography-tandem mass spectrometry. All polyunsaturated fatty acids quantified in milk were elevated during coliform mastitis with linoleic acid being the most abundant. Oxylipids synthesized through the lipoxygenase and cytochrome P450 pathways accounted for the majority of the oxylipid biosynthesis. This study demonstrated a complex and diverse oxylipid network, most pronounced at the level of the mammary gland. Substrate availability, biosynthetic pathways, and degree of metabolism influence the biosynthesis of oxylipids during bovine coliform mastitis. Further studies are required to identify targets for novel interventions that modulate oxylipid biosynthesis during coliform mastitis to optimize inflammation.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, East Lansing 48824.
authors.forename: Vengai
authors.initials: V
authors.lastname: Mavangira
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, East Lansing 48824.
authors.forename: Jeffery C
authors.initials: JC
authors.lastname: Gandy
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing 48824.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, East Lansing 48824.
authors.forename: Valerie E
authors.initials: VE
authors.lastname: Ryman
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing 48824; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing 48824.
authors.forename: A
authors.initials: A
authors.lastname: Daniel Jones
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, East Lansing 48824. Electronic address: sordillo@msu.edu.
authors.forename: Lorraine M
authors.initials: LM
authors.lastname: Sordillo
mesh_terms: D000208:Acute Disease; D000818:Animals; D016022:Case-Control Studies; D002417:Cattle; D002853:Chromatography, Liquid; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000662:veterinary; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D005260:Female; D019787:Linoleic Acid / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D008414:Mastitis, Bovine / Q000188:drug therapy* / Q000382:microbiology; D008892:Milk / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D019787:Linoleic Acid
keywords: inflammation; lipids; mastitis; oxylipid