title: Enhanced fluorescence detection of miRNA-16 on a photonic crystal.
abstract: We report a novel sensing method for fluorescence-labelled microRNAs (miRNAs) spotted on an all-dielectric photonic structure. Such a photonic structure provides an enhanced excitation and a directional beaming of the emitted fluorescence, resulting in a significant improvement of the overall signal collected. As a result, the Limit of Detection (LoD) is demonstrated to decrease by a factor of about 50. A compact read-out system allows a wide-field imaging-based detection, with little or no optical alignment issues, which makes this approach particularly interesting for further development for example in microarray-type bioassays.
authors.affiliation: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy. emiliano.descrovi@polito.it.
authors.forename: F
authors.initials: F
authors.lastname: Frascella
authors.forename: S
authors.initials: S
authors.lastname: Ricciardi
authors.forename: L
authors.initials: L
authors.lastname: Pasquardini
authors.forename: C
authors.initials: C
authors.lastname: Potrich
authors.forename: A
authors.initials: A
authors.lastname: Angelini
authors.forename: A
authors.initials: A
authors.lastname: Chiadò
authors.forename: C
authors.initials: C
authors.lastname: Pederzolli
authors.forename: N
authors.initials: N
authors.lastname: De Leo
authors.forename: P
authors.initials: P
authors.lastname: Rivolo
authors.forename: C F
authors.initials: CF
authors.lastname: Pirri
authors.forename: E
authors.initials: E
authors.lastname: Descrovi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C505362:MIRN16 microRNA, human; D035683:MicroRNAs