title: In vitro evaluation of the cytotoxicity and modulation of mechanisms associated with inflammation induced by perfluorooctanesulfonate and perfluorooctanoic acid in human colon myofibroblasts CCD-18Co.
abstract: Perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA) are the most notable members of an emerging class of persistent organic pollutants (POPs), poly- and perfluoroalkyl acids (PFASs). In this study, the CCD-18Co myofibroblasts were selected as a cell model to investigate the cytotoxic effects of PFOS and PFOA. The aim was to perform an in vitro evaluation of the ability of these compounds to induce cytotoxicity and modulate mechanisms associated with inflammation as measured by (i) colon fibroblasts viability, (ii) colon fibroblasts proliferation, and (iii) IL-6 production. The data provided in this study suggest that PFOS and PFOA can have cytotoxic potential and modulate processes associated with intestinal inflammation such as myofibroblasts proliferation and IL-6 production at concentrations similar to those detected in vivo. Our results also highlight the influence of culture serum concentration in cytotoxic in vitro studies, which should be considered in future toxicity studies involving PFOS and PFOA. The results contribute to a better knowledge of the effects of PFOS and PFOA in human cells, a phenomenon still not fully examined.
authors.affiliation: Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. Electronic address: juan.gimenez-bastida@pan.olsztyn.pl.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Giménez-Bastida
authors.affiliation: Malopolska Centre of Food Monitoring, Faculty of Food Technology, University of Agriculture in Krakow, Balicka St. 122, 30-149 Krakow, Poland. Electronic address: m.surma@ur.krakow.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Surma
authors.affiliation: Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. Electronic address: h.zielinski@pan.oldztyn.pl.
authors.forename: Henryk
authors.initials: H
authors.lastname: Zieliński
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D002210:Caprylates / Q000633:toxicity*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003106:Colon / Q000166:cytology; D004785:Environmental Pollutants / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D058628:Myofibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D017738:Alkanesulfonic Acids; D002210:Caprylates; D004785:Environmental Pollutants; D005466:Fluorocarbons; C508600:IL6 protein, human; D015850:Interleukin-6; C023036:perfluorooctanoic acid; C076994:perfluorooctane sulfonic acid
keywords: Inflammation; Myofibroblasts CCD18-Co; Perfluorooctanesulfonate; Perfluorooctanoic acid; Polyfluoroalkyl and perfluoroalkyl acids