title: Glucose-induced glutathione reduction in mitochondria is involved in the first phase of pancreatic β-cell insulin secretion.
abstract: Glucose can acutely reduce mitochondrial glutathione redox state in rat islets. However, whether glucose-stimulated mitochondrial glutathione redox state relates to glucose-stimulated insulin secretion (GSIS) remains unknown. We used genetically encoded redox-sensitive GFPs to target the mitochondria to monitor glutathione redox changes during GSIS in rat pancreatic β-cells. The results showed that mitochondrial glutathione was more reduced during GSIS, whereas inhibition of this glutathione reduction impaired insulin secretion. In isolated rat pancreatic islets glutathione reduction in mitochondria and the first phase of GSIS were concurrence at the early stage of glucose-stimulation. Our results suggest that the glucose-induced glutathione reduction in mitochondria is primarily required for the first phase of GSIS.
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, Shanghai 200092, China. Electronic address: xjliu@sibcb.ac.cn.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: shan@sibcb.ac.cn.
authors.forename: Shuai
authors.initials: S
authors.lastname: Han
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: yyang01@sibcb.ac.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, Shanghai 200092, China. Electronic address: jhkang@tongji.edu.cn.
authors.forename: Jiuhong
authors.initials: J
authors.lastname: Kang
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China; Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China. Electronic address: wujr@sibs.ac.cn.
authors.forename: Jiarui
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D054477:Glutaredoxins / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C516007:Glrx protein, rat; D054477:Glutaredoxins; D007328:Insulin; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005978:Glutathione; D005947:Glucose
keywords: Glutathione; Insulin secretion; Mitochondria; Pancreatic beta-cell; Redox