title: Robust optical fiber patch-cords for in vivo optogenetic experiments in rats.
abstract: In vivo optogenetic experiments commonly employ long lengths of optical fiber to connect the light source (commonly a laser) to the optical fiber implants in the brain. Commercially available patch cords are expensive and break easily. Researchers have developed methods to build these cables in house for in vivo experiments with rodents [1-4]. However, the half-life of those patch cords is greatly reduced when they are used with behaving rats, which are strong enough to break the delicate cable tip and to bite through the optical fiber and furcation tubing. Based on [3] we have strengthened the patch-cord tip that connects to the optical implant, and we have incorporated multiple layers of shielding to produce more robust and resistant cladding. Here, we illustrate how to build these patch cords with FC or M3 connectors. However, the design can be adapted for use with other common optical-fiber connectors. We have saved time and money by using this design in our optical self-stimulation experiments with rats, which are commonly several months long and last four to eleven hours per session. The main advantages are: •Long half-life.•Resistant to moderate rodent bites.•Suitable for long in vivo optogenetic experiments with large rodents.
authors.affiliation: Center for Studies in Behavioral Neurobiology (CSBN)/Groupe de recherche en neurobiologie comportementale, Department of Psychology. Concordia University, 7141 Sherbrooke Street West, Science Pavilion, room #244, H4B 1R6 Montréal, QC, Canada.
authors.forename: Ivan
authors.initials: I
authors.lastname: Trujillo-Pisanty
authors.affiliation: Center for Studies in Behavioral Neurobiology (CSBN)/Groupe de recherche en neurobiologie comportementale, Department of Psychology. Concordia University, 7141 Sherbrooke Street West, Science Pavilion, room #244, H4B 1R6 Montréal, QC, Canada.
authors.forename: Christian
authors.initials: C
authors.lastname: Sanio
authors.affiliation: Center for Studies in Behavioral Neurobiology (CSBN)/Groupe de recherche en neurobiologie comportementale, Department of Psychology. Concordia University, 7141 Sherbrooke Street West, Science Pavilion, room #244, H4B 1R6 Montréal, QC, Canada.
authors.forename: Nadia
authors.initials: N
authors.lastname: Chaudhri
authors.affiliation: Center for Studies in Behavioral Neurobiology (CSBN)/Groupe de recherche en neurobiologie comportementale, Department of Psychology. Concordia University, 7141 Sherbrooke Street West, Science Pavilion, room #244, H4B 1R6 Montréal, QC, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Shizgal
keywords: Behavior; Construction of resistant optical-fiber cables for behavioral optogenetics; Optical fiber; Optogenetics