title: Nucleic Acid Aptamers: An Emerging Tool for Biotechnology and Biomedical Sensing.
abstract: Detection of small molecules or proteins of living cells provides an exceptional opportunity to study genetic variations and functions, cellular behaviors, and various diseases including cancer and microbial infections. Our aim in this review is to give an overview of selected research activities related to nucleic acid-based aptamer techniques that have been reported in the past two decades. Limitations of aptamers and possible approaches to overcome these limitations are also discussed.
authors.affiliation: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA 92093-0407, USA. tiku@eng.ucsd.edu.
authors.forename: Ti-Hsuan
authors.initials: TH
authors.lastname: Ku
authors.affiliation: Materials Science and Engineering Program, University of California San Diego, La Jolla, CA 92093-0418, USA. tiz022@eng.ucsd.edu.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA 92093-0407, USA. luohuabox@gmail.com.|College of Basic Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China. luohuabox@gmail.com.|College of Manufacturing Science and Engineering, Sichuan University, Chengdu 610041, China. luohuabox@gmail.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Bioengineering, University of California San Diego, La Jolla, CA 92093-0412, USA. tmyen@eng.ucsd.edu.
authors.forename: Tony M
authors.initials: TM
authors.lastname: Yen
authors.affiliation: Chemical Engineering Program, University of California San Diego, La Jolla, CA 92093-0448, USA. pingwei320@gmail.com.
authors.forename: Ping-Wei
authors.initials: PW
authors.lastname: Chen
authors.affiliation: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA 92093-0407, USA. yuh054@ucsd.edu.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA 92093-0407, USA. ylo@ece.ucsd.edu.
authors.forename: Yu-Hwa
authors.initials: YH
authors.lastname: Lo
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide; D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008081:Liposomes; D051379:Mice; D012313:RNA / Q000032:analysis / Q000737:chemistry; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D008081:Liposomes; D012313:RNA; D004247:DNA
keywords: DNA; RNA; SELEX; aptamer; biomarkers; liposome; polymer