title: The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing.
abstract: INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants.
authors.affiliation: Laboratorio de Agrobiotecnología, Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA-CONICET-UBA), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Carlotto
authors.affiliation: Laboratorio de Agrobiotecnología, Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA-CONICET-UBA), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Sonia
authors.initials: S
authors.lastname: Wirth
authors.affiliation: Laboratorio de Agrobiotecnología, Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA-CONICET-UBA), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Furman
authors.affiliation: Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA) - CONICET - Partner Institute of the Max Planck Society, Buenos Aires, Argentina.
authors.forename: Nazarena
authors.initials: N
authors.lastname: Ferreyra Solari
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France.
authors.forename: Federico
authors.initials: F
authors.lastname: Ariel
authors.affiliation: Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Crespi
authors.affiliation: Laboratorio de Agrobiotecnología, Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA-CONICET-UBA), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Ken
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002736:Chloroplasts / Q000201:enzymology; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D007438:Introns / Q000235:genetics; D009154:Mutation; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D031425:Plasmodesmata / Q000378:metabolism*; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D018747:RNA, Chloroplast / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018747:RNA, Chloroplast; C523445:INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases
keywords: Retrograde signals; cell-to-cell communication; chloroplast