title: Altered glutathione system is associated with the presence of distal symmetric peripheral polyneuropathy in type 2 diabetic subjects.
abstract: Distal symmetric peripheral polyneuropathy (DSPN) is a highly prevalent complication of diabetes. However, underlying pathophysiological mechanisms are multiple and not well understood. The aim of our study was to analyze the oxidative stress levels in circulating mononuclear cells by measuring the glutathione system, malondialdehyde and oxidized-LDL, in 60 type 2 diabetic patients from a well-characterized cohort of 196 type 2 diabetic patients. Using a nested case-control design, we studied 30 type 2 diabetic patients with distal symmetric polyneuropathy and 30 diabetic controls without this complication, according to the Neuropathy Disability Score. We have found that diabetic patients with distal symmetric polyneuropathy showed significantly lower values of reduced glutathione (GSH) and reduced glutathione/oxidized glutathione (GSH/GSSG) ratio. These data indicate an increased consumption of glutathione in mononuclear cells from patients with distal symmetric polyneuropathy. No significant differences were found in malondialdehyde or in oxidized-LDL levels comparing both groups. These data show an altered glutathione response in circulating monocytes from diabetic patients with distal symmetric polyneuropathy.
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain.
authors.forename: Mercedes Molina
authors.initials: MM
authors.lastname: Mendez
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Folgado
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Valencia and CIBER de Obesidad, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Tormo
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Artero
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Ascaso
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain; CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Barcelona, Spain; Laboratorio de Estudios Genéticos, Fundación para la Investigación. Instituto de Investigación INCLIVA, Valencia, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Martinez-Hervás
authors.affiliation: CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Barcelona, Spain; Laboratorio de Estudios Genéticos, Fundación para la Investigación. Instituto de Investigación INCLIVA, Valencia, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Chaves
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain; CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Barcelona, Spain; Laboratorio de Estudios Genéticos, Fundación para la Investigación. Instituto de Investigación INCLIVA, Valencia, Spain; Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Ascaso
authors.affiliation: Service of Endocrinology and Nutrition, Hospital Clínico Universitario de Valencia, Valencia, Spain; CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Barcelona, Spain; Laboratorio de Estudios Genéticos, Fundación para la Investigación. Instituto de Investigación INCLIVA, Valencia, Spain; Department of Medicine, University of Valencia, Valencia, Spain. Electronic address: jtreal@uv.es.
authors.forename: Jose T
authors.initials: JT
authors.lastname: Real
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000175:diagnosis / Q000188:drug therapy; D003929:Diabetic Neuropathies / Q000453:epidemiology / Q000209:etiology* / Q000503:physiopathology; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D015994:Incidence; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D011379:Prognosis; D018570:Risk Assessment; D012720:Severity of Illness Index
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D005978:Glutathione
keywords: Glutathione; Neuropathy disability score; Oxidative stress; Peripheral polyneuropathy; Type 2 diabetes mellitus