title: Antitumor effects evaluation of a novel porphyrin derivative in photodynamic therapy.
abstract: In this paper, the antitumor activity of a novel porphyrin-based photosensitizer 5,10,15,20-tetrakis[(5-diethylamino)pentyl] porphyrin (TDPP) was reported in vitro and in vivo. The photophysical and cellular properties of TDPP were investigated. The singlet oxygen generation quantum yield of TDPP was detected; it showed a high singlet oxygen quantum yield of 0.52. The intracellular distribution of photosensitizer was detected with laser scanning confocal microscopy. The efficiency of TDPP-photodynamic therapy (PDT) in vitro was analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and in situ trypan blue exclusion test. Treated with a 630-nm laser, TDPP can kill cultured human esophageal cancer cell line (Eca-109) cells and reduce the growth of Eca-109 xenograft tumors significantly in BABL/c nude mice. And histopathological study was also used to confirm the antitumor effect. It has the perspective to be developed as a new antitumor drug in photodynamic therapy and deserves further investigation.
authors.affiliation: Yiwu City Central Hospital, Zhejiang, 322000, People's Republic of China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Li
authors.affiliation: Yiwu City Central Hospital, Zhejiang, 322000, People's Republic of China.
authors.forename: Zhong-Ming
authors.initials: ZM
authors.lastname: Wu
authors.affiliation: Division of organic chemistry and biochemistr, Rudjer Boskovic Institute, Zagreb, Croatia.
authors.forename: Davor
authors.initials: D
authors.lastname: Magetic
authors.affiliation: Department of Pharmaceutical Science & Technology, College of Chemistry and Biology, Donghua University, Shanghai, 201620, People's Republic of China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Science & Technology, College of Chemistry and Biology, Donghua University, Shanghai, 201620, People's Republic of China. zlchen1967@yahoo.com.
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D051379:Mice; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage*; D023041:Xenograft Model Antitumor Assays
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins
keywords: Photodynamic therapy; Photosensitizer; Porphyrin; TDPP; Tumor