title: New flavonoid from Patrinia villosa.
abstract: BACKGROUND
  Patrinia villosa (Thunb.) Juss (Valerianaceae) is an important ancient herbal medicine widely used for inflammation, wound healing, and abdominal pain. But little is known of the phytochemical constituents of this herbal plant.
  
  
  OBJECTIVE
  The objective of this study is to isolate and identify the bioactive components from P. villosa.
  
  
  METHODS
  A 70% EtOH extract of P. villosa was subjected to normal-phase silica, ODS silica gel column chromatography, and semi-preparative HPLC chromatography after partitioned successively with light petroleum, dichloromethane and n-BuOH. Chemical structures of the compounds were elucidated by spectroscopic methods including UV, 1D-NMR, 2D-NMR, HR-ESI-MS, and CD spectra. The cytotoxic activity of the new component was determined with the SMMC-7721 cell line using the MTT method after incubation for 48 h.
  
  
  RESULTS
  A new flavonoid named patriniaflavanone A (1) along with four known compounds was isolated from P. villosa. The four known compounds were identified as luteolin 7-O-glucuronide-6″-methyl ester (2), p-hydroxyphenylacetic acid methyl ester (3), trans-caffeic acid (4), and trans-caffeic acid methylate (5) by comparison of their spectral data with the reported data. The IC50 value of patriniaflavanone A (1) on SMMC-7721 was 61.27 μM.
  
  
  CONCLUSIONS
  This is the first report on the isolation and identification of patriniaflavanone A (1), and compounds 2-5 were isolated for the first time from the title plant. Patriniaflavanone A (1) exhibited moderate cytotoxic activity.
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Tan
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ji
authors.affiliation: a Center of Research on Life Science and Environmental Science, Harbin University of Commerce , Harbin , PR China.
authors.forename: Wen-Lan
authors.initials: WL
authors.lastname: Li
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D032405:Patrinia / Q000737:chemistry*; D008517:Phytotherapy; D018515:Plant Leaves; D010946:Plants, Medicinal; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids
keywords: Caffeic acid; cytotoxic activity; nuclear magnetic resonance spectroscopy