title: Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles.
abstract: Fragmin/protamine microparticles (F/P MPs) approximately 0.5-1 µM in diameter were prepared by the simple mixing of fragmin with protamine. This study investigated the effects of F/P MP-containing collagen gels as a hormone carrier on the formation of antral follicle-like structures and on the development of growing bovine oocytes. The supplementation of F/P MPs in collagen gels contributed to the beneficial effects of follicle stimulating hormone (FSH) on the formation and size of antral follicle-like structures. The F/P MPs may serve as potential hormone carriers for the growth of cultured bovine oocytes from early antral follicles.
authors.affiliation: a Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science , Yamaguchi University , Yamaguchi , Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Morita
authors.forename: Ni Wayan Kurniani
authors.initials: NW
authors.lastname: Karja
authors.forename: Lanh Thi Kim
authors.initials: LT
authors.lastname: Do
authors.forename: Fuminori
authors.initials: F
authors.lastname: Tanihara
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Takeshige
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000818:Animals; D002417:Cattle; D054885:Cumulus Cells / Q000187:drug effects*; D017985:Dalteparin / Q000494:pharmacology*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D009865:Oocytes / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development*; D010316:Particle Size; D011479:Protamines / Q000494:pharmacology*
chemical_list: D011479:Protamines; D017985:Dalteparin
keywords: FSH; Follicular formation; In vitro culture; Nanoparticles; Oocyte