title: Aflatoxin B1 in eggs and chicken livers by dispersive liquid-liquid microextraction and HPLC.
abstract: A rapid, low-cost and simple technique has been developed for the determination of aflatoxin B1 (AFB1) in eggs and livers using high-performance liquid chromatography (HPLC) with UV detection. In this study, the presence of AFB1 was investigated in 150 eggs and 50 chicken livers from the local market of Tabriz, Iran. AFB1 was extracted with a mixture of acetonitrile:water (80:20) and cleaned up by dispersive liquid-liquid microextraction which is a very economical, fast and sensitive method. AFB1 was quantified by HPLC-UV without need for any complex derivatisation in samples to enhance the detection. The results showed that 72% of the liver and 58% of the egg samples were contaminated with AFB1 ranging from 0.30 to 16.36 µg kg (̶1). limit of detection and limit of quantification for AFB1 were 0.08 and 0.28 µg kg (̶ 1), respectively. The proposed method is suitable for fast analysing of AFB1 in egg and liver samples.
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Faculty of Nutrition , Tabriz University of Medical Sciences , Tabriz , Iran.|c Students' Research Committee, Faculty of Nutrition , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Amirkhizi
authors.affiliation: b Faculty of Nutrition , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Seyed Rafie
authors.initials: SR
authors.lastname: Arefhosseini
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Ansarin
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|d Faculty of Pharmacy , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mahboob
authors.initials: M
authors.lastname: Nemati
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004032:Diet; D004531:Eggs / Q000032:analysis*; D004781:Environmental Exposure; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D007492:Iran; D059627:Liquid Phase Microextraction / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*
chemical_list: D016604:Aflatoxin B1
keywords: HPLC; aflatoxin B1; dispersive liquid–liquid microextraction; egg; liver