title: Increased expression of Notch 1 in a rat liver transplantation model.
abstract: Liver transplantation is the standard treatment for end‑stage liver failure; however, rejection can result in allograft failure. In order to investigate the role of Notch 1 during rejection, the present study evaluated Notch 1 expression, as well as the levels of immune reactivity, in rat liver allografts. A heterotopic liver transplantation model was established using Dark Agouti (DA) rats as donors and Lewis rats as recipients (DA/Lewis), with DA recipient rats serving as controls (DA/DA). The concentration levels of immune reactivity markers and serum Notch 1 were measured on days 3, 5, and 7. The overall survival was significantly shorter (<10 days) in the DA/Lewis group, as compared with the DA/DA group (P<0.0001). The concentration levels of serum alanine aminotransferase and total bilirubin were significantly higher 5 and 7 days following transplantation in the DA/Lewis group, as compared with the DA/DA group (P<0.001). The concentration levels of serum Notch 1 were significantly higher in the DA/Lewis group, as compared with the DA/DA group on days 3, 5, and 7 following transplantation (P<0.0001). These results indicate that the expression levels of serum Notch 1 significantly increase during liver allograft rejection, suggesting that Notch 1 is involved in the mechanism underlying liver allograft rejection. Notch 1 may serve as a marker of acute rejection in a rat liver transplantation model.
authors.affiliation: Department of Pathology, School of Basic Medical Sciences of Fujian Medical University, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Provincial Clinical Medical College, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Wubing
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Pathology, School of Basic Medical Sciences of Fujian Medical University, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Kaida
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences of Fujian Medical University, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Pathology, School of Basic Medical Sciences of Fujian Medical University, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Pathology, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian 350025, P.R. China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathology, School of Basic Medical Sciences of Fujian Medical University, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Huang
mesh_terms: D000818:Animals; D015415:Biomarkers; D015870:Gene Expression; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D016031:Liver Transplantation; D008297:Male; D023421:Models, Animal; D051381:Rats; D051881:Receptor, Notch1 / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D051881:Receptor, Notch1