title: [Preventive effect of misoprostol against nonsteroidal anti-inflammatory drug-induced enteropathy in mice].
abstract: OBJECTIVE
  To investigate the preventive effect of misoprostol against non-steroidal anti-inflammatory drug (NSAID)-induced intestinal injury in mice.
  
  
  METHODS
  NSAID-induced intestinal injury model was established through diclofenac sodium. Sixty specific-pathogen-free (SPF) BABL/c male mice were randomly divided into the following five groups: normal, model and three misoprostol groups with different concentrations (200, 400, 800 μg/kg). Misoprostol was given to aforementioned three misoprostol groups by gavage once a day for 6 days. In the fourth day afternoon, 5 mg/kg (10 mL/kg) diclofenac was fed to all mice by gavage except for normal group. On the seventh day, all mice were sacrificed and intestinal permeability was detected using FITC labeled dextran. The intestinal tissues were taken for detecting the mRNA and protein expressions of intestinal glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP) and tumor necrosis factor alpha (TNF-α) through HE staining, reverse transcription PCR and Western blotting, respectively.
  
  
  RESULTS
  Compared with the normal group, intestinal mucosa in the model group was seriously damaged and intestinal permeability significantly increased. The intestinal mucosal villus degeneration, necrosis, shedding, and inflammatory cell infiltration occurred in the model group. Yet, intestinal mucosal injury in different misoprostol groups was less severe. Their intestinal mucosal permeability was improved. The expressions of GRP78 protein and TNF-α, CHOP mRNAs on intestine were significantly reduced compared with those of the model group.
  
  
  CONCLUSIONS
  Misoprostol has preventive effect against NSAID-induced intestinal diseases.
authors.affiliation: Department of Gastroenterology, Third Clinical Medical College, Gezhouba Central Hospital, Yichang 443002, China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Gastroenterology, Third Clinical Medical College, Gezhouba Central Hospital, Yichang 443002, China.
authors.forename: Faming
authors.initials: F
authors.lastname: Wu
authors.affiliation: Institute of Molecular Biology, China Three Gorges University, Yichang 443002, China.
authors.forename: Zhaozhao
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Institute of Molecular Biology, China Three Gorges University, Yichang 443002, China.
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Third Clinical Medical College, Gezhouba Central Hospital, Yichang 443002, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Gastroenterology, Third Clinical Medical College, Gezhouba Central Hospital, Yichang 443002, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics; D007410:Intestinal Diseases / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016595:Misoprostol / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C497250:Ddit3 protein, mouse; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016595:Misoprostol; D051742:Transcription Factor CHOP