title: Evaluation of Dye Compounds' Decolorization Capacity of SelectedH. haematococcaandT. harzianumStrains by Principal Component Analysis (PCA).
abstract: The selected strains of microscopic fungi,Haematonectria haematococca(BwIII43, K37) andTrichoderma harzianum(BsIII33), decolorized the following monoathraquinone dyes with different efficiency: 0.03 % Alizarin Blue Black B, 0.01 % Carminic Acid, 0.01 % Poly R-478, and 0.2 % post-industrial lignin. The most effective was the removal of 0.03 % Alizarin Blue Black B (50-60 %) and 0.01 % Carminic Acid (55-85 %). The principal component analysis (PCA) method was applied to determine the main enzyme responsible for the biodecolorization process of the dye substrates and indicated that horseradish-type (HRP-like), lignin (LiP), and manganese-dependent (MnP) peroxidases were responsible for the decolorization of anthraquinone dyes by the strains tested. The participation of particular enzymes in the decolorization of monoanthraquinone dyes ranged from 44.48 to 51.70 % for 0.01 % Carminic Acid and from 38.46 to 61.12 % for Poly R-478. The highest precipitation in decolorization of these dyes showed HRP-like peroxidase, respectively, 54-74 and 70-95 %. The degree of decolorization of 0.2 % post-industrial lignin by the selected strains ofH. haematococcaandT. harzianumamounted to 58.20, 61.38, and 65.13 %, respectively. The rate of 0.2 % post-industrial lignin decolorization was conditioned by the activity of HRP-like (71-90 %) and LiP (87-94 %) peroxidases.
authors.affiliation: Department of Environmental Microbiology, Laboratory of Mycology, The University of Life Sciences, ul. Leszczyńskiego 7, 20-069 Lublin, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Rybczyńska
authors.affiliation: Department of Environmental Microbiology, Laboratory of Mycology, The University of Life Sciences, ul. Leszczyńskiego 7, 20-069 Lublin, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Korniłłowicz-Kowalska
keywords: Anthraquinone dyes; Biodecolorization; H. haematococca; Peroxidases; Post-industrial lignin; T. harzianum