title: Interleukin-33 promotes inflammatory cytokine production in chronic airway inflammation.
abstract: Interleukin (IL)-33, belonging to the IL-1 family, is a novel cytokine that plays an important role in several chronic inflammatory diseases. Its role in chronic airway inflammation that develops into COPD is widely unknown. To determine this, we identified the expression of IL-33 in human bronchial epithelial layer and detected the inflammatory effects of IL-33 stimulation and the relative signaling pathways in human bronchial epithelial (HBE) cells and peripheral blood mononuclear cells (PBMCs), respectively. In this study, the expression of IL-33 in human bronchial epithelial layer was upregulated in COPD patients compared with normal controls. The expressions of IL-6 and IL-8 were also increased in both HBE cells and PBMCs, stimulated by IL-33 alone or combining the cigarette smoke extract (CSE). And the increased expressions could be partially blocked by ST2-Fc and IL-1RacP-Fc in both HBE cells and PBMCs. The p42/p44 ERK inhibitor in HBE cells and the p38 MAPK inhibitor in PBMCs exerted similar effects. Our data showed that IL-33 could induce and enhance the expression of IL-6 and IL-8 in HBE cells and PBMCs of COPD patients via ST2/IL-1RacP pathway and MAPKs pathway. Thus, the IL-33 is a promoter of chronic airway inflammation that contributes to COPD development.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.|Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.|Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.
authors.forename: Junling
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.|Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.|Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.|Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.
authors.forename: Jungang
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.|Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P. R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000368:Aged; D001991:Bronchitis / Q000378:metabolism*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D000067596:Interleukin-33 / Q000502:physiology*; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism; D011897:Random Allocation
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D000067596:Interleukin-33
keywords: Interleukin-33; cellules mononucléaires du sang périphérique; chronic obstructive pulmonary disease; cigarette smoke extract; extrait de fumée de cigarette; human bronchial epithelial; interleuline-33; maladie pulmonaire obstructive chronique; peripheral blood mononuclear cell; épithélium bronchique humain