title: [Application of Coagulant in the Analysis of Lycorine and Galanthamine in Processed Foods].
abstract: Analytical method by HPLC and LC-MS/MS for determining lycorine and galanthamine in processed food was newly developed. In this method, coagulant which has never been used in food analysis was applied on cleanup process. With coagulant approach, removal of interfering substances on determination for analytes was easily achieved. The method using HPLC showed recovery of 95.4-102.9% on both analytes with repeatability of less than 2.9% and reproducibility of less than 2.9%. The method using LC-MS/MS showed recovery of 97.4-107.6% with repeatability of less than 5.7% and reproducibility of less than 5.7%. On HPLC method, limit of quantification for lycorine was 0.004 g/kg and that of galanthamine was 0.006 g/kg. On LC-MS/MS method, limit of quantification for lycorine was 0.0008 g/kg and that of galanthamine was 0.0005 g/kg.
authors.affiliation: Nagoya City Public Health Research Institute.
authors.forename: Jun
authors.initials: J
authors.lastname: Sugiura
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Noguchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003029:Coagulants; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D005702:Galantamine / Q000032:analysis*; D010617:Phenanthridines / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047151:Amaryllidaceae Alkaloids; D003029:Coagulants; D010617:Phenanthridines; D005702:Galantamine; C015330:lycorine