title: Comparison of different blood compartments for the detection of circulating DNA using a rat model of Pneumocystis pneumonia.
abstract: Pneumocystis is mostly found in the alveolar spaces, but circulation of viable organisms also occurs and suggests that the detection of DNA in blood could be used as a noninvasive procedure to improve the diagnosis of Pneumocystis pneumonia (PcP). In order to determine the optimal compartment for Pneumocystis DNA detection, we used a rat model of PcP and tested the presence of Pneumocystis with a quantitative mtLSU targeting real-time PCR in four blood compartments: whole blood, clot, serum and Platelet-Rich-Plasma (PRP). All samples from 4 Pneumocystis-free control rats were negative. Pneumocystis was detected in 79, 64, 57, and 57% of samples from 14 PcP rats, respectively, but DNA release was not related to pulmonary loads. These data confirm the potential usefulness of Pneumocystis DNA detection in the blood for PcP diagnosis and suggest that whole blood could be the most appropriate compartment for Pneumocystis detection.
authors.affiliation: Centre d'Infection et d'Immunité de Lille (CIIL), Inserm U1019 - CNRS UMR 8204 - Université de Lille, IFR142, Institut Pasteur de Lille, France Laboratoire de Parasitologie-Mycologie, CHRU de Lille & Faculté de Médecine de Lille - Université de Lille, France emilie.frealle-2@univ-lille2.fr.
authors.forename: E
authors.initials: E
authors.lastname: Fréalle
authors.affiliation: Centre d'Infection et d'Immunité de Lille (CIIL), Inserm U1019 - CNRS UMR 8204 - Université de Lille, IFR142, Institut Pasteur de Lille, France.
authors.forename: N
authors.initials: N
authors.lastname: Gantois
authors.affiliation: Centre d'Infection et d'Immunité de Lille (CIIL), Inserm U1019 - CNRS UMR 8204 - Université de Lille, IFR142, Institut Pasteur de Lille, France Laboratoire de Parasitologie, Faculté de Pharmacie de Lille - Université de Lille, France.
authors.forename: C M
authors.initials: CM
authors.lastname: Aliouat-Denis
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHRU de Lille & Faculté de Médecine de Lille - Université de Lille, France.
authors.forename: S
authors.initials: S
authors.lastname: Leroy
authors.affiliation: Laboratoire d'Hématologie & EA2693 Interface sang - vaisseaux et réparation cardiovasculaire, CHRU de Lille & Faculté de Médecine de Lille - Université de Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Zawadzki
authors.affiliation: University of Applied Sciences Tyrol, Innsbruck, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Perkhofer
authors.affiliation: Centre d'Infection et d'Immunité de Lille (CIIL), Inserm U1019 - CNRS UMR 8204 - Université de Lille, IFR142, Institut Pasteur de Lille, France Laboratoire de Parasitologie, Faculté de Pharmacie de Lille - Université de Lille, France.
authors.forename: E M
authors.initials: EM
authors.lastname: Aliouat
authors.affiliation: Centre d'Infection et d'Immunité de Lille (CIIL), Inserm U1019 - CNRS UMR 8204 - Université de Lille, IFR142, Institut Pasteur de Lille, France Laboratoire de Parasitologie-Mycologie, CHRU de Lille & Faculté de Médecine de Lille - Université de Lille, France.
authors.forename: E
authors.initials: E
authors.lastname: Dei-Cas
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology*; D004271:DNA, Fungal / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004195:Disease Models, Animal; D045363:Pneumocystis carinii / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D011923:Rats, Nude; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
keywords: Pneumocystis; clot; diagnosis; platelet-rich-plasma; real-time PCR; serum; whole blood