title: Sirtuin 1 suppresses nuclear factor κB induced transactivation and pro-inflammatory cytokine expression in cat fibroblast cells.
abstract: Nuclear factor κB (NF-κB) is a key factor in the development of chronic inflammation and is deeply involved in age-related and metabolic diseases development. These diseases have become a serious problem in cats. Sirtuin 1 (SIRT1) is associated with aging and metabolism through maintaining inflammation via NF-κB. In addition, fibroblasts are considered an important factor in the development of chronic inflammation. Therefore, we aimed to examine the effect of cat SIRT1 (cSIRT1) on NF-κB in cat fibroblast cells. The up-regulation of NF-κB transcriptional activity and pro-inflammatory cytokine mRNA expression by p65 subunit of NF-κB and lipopolysaccharide was suppressed by cSIRT1 in cat fibroblast cells. Our findings show that cSIRT1 is involved in the suppression of inflammation in cat fibroblast cells.
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takemitsu
authors.forename: Makoto
authors.initials: M
authors.lastname: Habara
authors.forename: Nobuko
authors.initials: N
authors.lastname: Mori
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
mesh_terms: D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D016207:Cytokines; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D056564:Sirtuin 1 / Q000494:pharmacology*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D012333:RNA, Messenger; D056564:Sirtuin 1