title: Apoptosis induced in MCF-7 human breast cancer cells by 2',4'-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated fromEugenia aqueaBurm f. leaves.
abstract: During a previous study that aimed to identify anticancer agents within primate-consumed plants, the present group identified thatEugenia aquea(E. aquea) possessed potential as a source of anticancer agents. The ethanol extract ofE. aquealeaves exhibited strong inhibitory activity against the proliferation of the human breast adenocarcinoma MCF-7 cell line. The inhibition of proliferation was determined using an MTT assay. The present study was performed to isolate the active compound within theE. aquealeaves that generated the aforementioned activity, and resulted in the isolation of 2',4'-dihydroxy-6-methoxy-3,5-dimethylchalcone, which was identified through the analysis of spectroscopic data. This compound was examined for its inhibitory activity against the MCF-7 cell line using a MTT assay, and the ability of 2',4'-dihydroxy-6-methoxy-3,5-dimethylchalcone to induce apoptosis through the activation of the poly(adenosine diphosphate-ribose) polymerase (PARP) protein was also investigated. The results of the present study revealed that the isolated compound inhibited cell proliferation in a dose-dependent manner, possessed an IC50of 74.5 µg/ml (250 µM) and promoted apoptosis via the activation of PARP. It was concluded that these results indicated a requirement for additional investigations into 2',4'-dihydroxy-6-methoxy-3,5-dimethylchalcone in order to provide a basis for the use of this compound in the management of cancer.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia.
authors.forename: Anas
authors.initials: A
authors.lastname: Subarnas
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia.
authors.forename: Ajeng
authors.initials: A
authors.lastname: Diantini
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia ; Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Rizky
authors.initials: R
authors.lastname: Abdulah
authors.affiliation: Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia.
authors.forename: Ade
authors.initials: A
authors.lastname: Zuhrotun
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Yuni E
authors.initials: YE
authors.lastname: Hadisaputri
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia ; Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Irma M
authors.initials: IM
authors.lastname: Puspitasari
authors.affiliation: Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Chiho
authors.initials: C
authors.lastname: Yamazaki
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
authors.affiliation: Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koyama
keywords: Eugenia aquea; apoptosis; cancer; primates