title: Catecholaminergic neurons projecting to the paraventricular nucleus of the hypothalamus are essential for cardiorespiratory adjustments to hypoxia.
abstract: Brainstem catecholamine neurons modulate sensory information and participate in control of cardiorespiratory function. These neurons have multiple projections, including to the paraventricular nucleus (PVN), which contributes to cardiorespiratory and neuroendocrine responses to hypoxia. We have shown that PVN-projecting catecholaminergic neurons are activated by hypoxia, but the function of these neurons is not known. To test the hypothesis that PVN-projecting catecholamine neurons participate in responses to respiratory challenges, we injected IgG saporin (control; n = 6) or anti-dopamine β-hydroxylase saporin (DSAP; n = 6) into the PVN to retrogradely lesion catecholamine neurons projecting to the PVN. After 2 wk, respiratory measurements (plethysmography) were made in awake rats during normoxia, increasing intensities of hypoxia (12, 10, and 8% O2) and hypercapnia (5% CO2-95% O2). DSAP decreased the number of tyrosine hydroxylase-immunoreactive terminals in PVN and cells counted in ventrolateral medulla (VLM; -37%) and nucleus tractus solitarii (nTS; -36%). DSAP produced a small but significant decrease in respiratory rate at baseline (during normoxia) and at all intensities of hypoxia. Tidal volume and minute ventilation (VE) index also were impaired at higher hypoxic intensities (10-8% O2; e.g., VE at 8% O2: IgG = 181 ± 22, DSAP = 91 ± 4 arbitrary units). Depressed ventilation in DSAP rats was associated with significantly lower arterial O2 saturation at all hypoxic intensities. PVN DSAP also reduced ventilatory responses to 5% CO2 (VE: IgG = 176 ± 21 and DSAP = 84 ± 5 arbitrary units). Data indicate that catecholamine neurons projecting to the PVN are important for peripheral and central chemoreflex respiratory responses and for maintenance of arterial oxygen levels during hypoxic stimuli.
authors.affiliation: Department of Biomedical Sciences.
authors.forename: T Luise
authors.initials: TL
authors.lastname: King
authors.affiliation: Department of Biomedical Sciences.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Ruyle
authors.affiliation: Department of Biomedical Sciences, Dalton Cardiovascular Research Center, and.
authors.forename: David D
authors.initials: DD
authors.lastname: Kline
authors.affiliation: Department of Biomedical Sciences, Dalton Cardiovascular Research Center, and.
authors.forename: Cheryl M
authors.initials: CM
authors.lastname: Heesch
authors.affiliation: Department of Biomedical Sciences, Dalton Cardiovascular Research Center, and Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri HasserE@missouri.edu.
authors.forename: Eileen M
authors.initials: EM
authors.lastname: Hasser
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001933:Brain Stem / Q000166:cytology / Q000502:physiology; D002395:Catecholamines / Q000502:physiology*; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors; D006339:Heart Rate / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology*; D006935:Hypercapnia / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000502:physiology; D015656:Respiratory Mechanics / Q000502:physiology*; D054789:Ribosome Inactivating Proteins, Type 1 / Q000494:pharmacology; D000076985:Saporins; D013686:Telemetry
chemical_list: D002395:Catecholamines; D054789:Ribosome Inactivating Proteins, Type 1; D004299:Dopamine beta-Hydroxylase; D000076985:Saporins
keywords: anti-dopamine β-hydroxylase saporin; blood pressure; brainstem; chemoreflex; ventilation