title: Revealing Three Stages of DNA-Cisplatin Reaction by a Solid-State Nanopore.
abstract: The dynamic structural behavior in DNA due to interaction with cisplatin is essential for the functionality of platinum-based anti-cancer drugs. Here we report a novel method to monitor the interaction progress in DNA-cisplatin reaction in real time with a solid-state nanopore. The interaction processes are found to be well elucidated by the evolution of the capture rate of DNA-cisplatin complex, which is defined as the number of their translocation events through the nanopore in unit time. In the first stage, the capture rate decreases rapidly due to DNA discharging as the positive-charged hydrated cisplatin molecules initially bond to the negative-charged DNA and form mono-adducts. In the second stage, by forming di-adducts, the capture rate increases as DNA molecules are softened, appears as the reduced persistence length of the DNA-cisplatin adducts. In the third stage, the capture rate decreases again as a result of DNA aggregation. Our study demonstrates a new single-molecule tool in exploring dynamic behaviors during drug-DNA reactions and may have future application in fast drug screening.
authors.affiliation: Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Shan
authors.affiliation: Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: 1] Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China [2] Collaborative Innovation Center of Quantum Matter, Beijing 100190, People's Republic of China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Bai
authors.affiliation: Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Pengye
authors.initials: P
authors.lastname: Wang
authors.affiliation: 1] Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China [2] Collaborative Innovation Center of Quantum Matter, Beijing 100190, People's Republic of China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Lu
mesh_terms: D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry; D008962:Models, Theoretical; D058608:Nanopores; D009690:Nucleic Acid Conformation
chemical_list: D018736:DNA Adducts; C057270:cisplatin-DNA adduct; D004247:DNA; D002945:Cisplatin