title: Enantioseparation tuned by solvent polarity on a β-cyclodextrin clicked chiral stationary phase.
abstract: The efficient enantioseparation of 26 racemates has been achieved with the perphenylcarbamoylated cyclodextrin clicked chiral stationary phase by screening the optimum composition of mobile phase in high-performance liquid chromatography. The chromatographic results indicate that both the retention and chiral resolution of racemates are closely related to the polarity of the mobile phases and the structures of analytes. The addition of alcohols can significantly tune the enantioseparation in normal-phase high-performance liquid chromatography. The addition of methanol and the ratio of ethanol/methanol or isopropanol/methanol played a key role on the resolution of flavonoids in ternary eluent systems. The chiral separation of flavonoids with pure organic solvent as mobile phase indicates the preferential order for chiral resolution is methanol>ethanol>isopropanol>n-propanol>acetonitrile.
authors.affiliation: College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Pang
authors.affiliation: College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Tang
keywords: Chiral separation; Chiral stationary phases; Cyclodextrins; High-performance liquid chromatography