title: Preparation of "dummy" l-phenylalanine molecularly imprinted microspheres by using ionic liquid as a template and functional monomer.
abstract: In this study, dummy imprinting technology was employed for the preparation of l-phenylalanine-imprinted microspheres. Ionic liquids were utilized as both a "dummy" template and functional monomer, and 4-vinylpyridine and ethylene glycol dimethacrylate were used as the assistant monomer and cross-linker, respectively, for preparing a surface-imprinted polymer on poly(divinylbenzene) microspheres. By the results obtained by theoretical investigation, the interaction between the template and monomer complex was improved as compared with that between the template and the traditional l-phenylalanine-imprinted polymer. The batch experiments indicated that the imprinting factor reached 2.5. Scatchard analysis demonstrated that the obtained "dummy" molecularly imprinted microspheres exhibited an affinity of 77.4 M·10-4, significantly higher that of a traditional polymer directly prepared by l-phenylalanine, which is in agreement with theoretical results. Competitive adsorption experiments also showed that the molecularly imprinted polymer with the dummy template effectively isolated l-phenylalanine from l-histidine and l-tryptophan with separation factors of 5.68 and 2.68, respectively. All these results demonstrated that the polymerizable ionic liquid as the dummy template could enhance the affinity and selectivity of molecularly imprinted polymer, thereby promoting the development of imprinting technology for biomolecules.
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guan
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Dongmen
authors.initials: D
authors.lastname: Song
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Qian
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Du
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Renyuan
authors.initials: R
authors.lastname: Song
authors.affiliation: Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Department of Chemistry, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710129, P. R. China.
authors.forename: Chaoli
authors.initials: C
authors.lastname: Wang
keywords: Dummy; Ionic liquids; L-phenylalanine; Molecular imprinting; Molecular simulation