title: Ascorbic acid and protein glycation in vitro.
abstract: The aim of the study was to compare the effects of ascorbic acid (AA) in vitro in the absence and in the presence of cell-dependent recycling. In a cell-free system, AA enhanced glycoxidation of bovine serum albumin (BSA) by glucose and induced BSA glycation in the absence of sugars. On the other hand, AA did not affect erythrocyte hemolysis, glycation of hemoglobin and erythrocyte membranes, and inactivation of catalase, protected against inactivation of acetylcholinesterase of erythrocytes incubated with high glucose concentrations and enhanced the loss of glutathione. These results can be explained by assumption that AA acts as a proglycating agent in the absence of recycling while is an antiglycating agent when metabolic recycling occurs.
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza St. 4, PL 35-601 Rzeszow, Poland. Electronic address: isadowska@poczta.fm.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sadowska-Bartosz
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza St. 4, PL 35-601 Rzeszow, Poland; Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D002474:Cell-Free System; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D012710:Serum Albumin, Bovine / Q000378:metabolism*
chemical_list: D012710:Serum Albumin, Bovine; D001205:Ascorbic Acid
keywords: Antioxidant; Ascorbic acid; Bovine serum albumin; Erythrocyte; Glycation