title: Gradual Rarefaction of Hematopoietic Precursors and Atrophy in a Depleted microRNA 29a, b and c Environment.
abstract: BACKGROUND
  The self-renewing ability of HSCs is fundamental for the maintenance of a pool of bone marrow precursors throughout the life of an individual. The genetic mechanisms underlying such a complex process are still poorly understood.
  
  
  CONCLUSIONS
  Here, we show that constitutive in vivo deletion of miR29ab1 leads to reduced number of HSCs and that miR29ab1 deficient bone marrow cannot repopulate the bone marrow of irradiated mice. An Affymetrix analysis of the miR29ab1 knockout mice identifies key proteins that could be responsible for this phenotype, as DNMT3a and b. Moreover, our findings reveal that whereas miR29b2c knockout mice do not exhibit any spontaneous abnormality, the double knock out--miR29ab1b2c--has marked generalized atrophy, raising the possibility that the two bi-cistrons might cooperate in order to maintain the stem cell number in general, not only limited to the bone marrow.
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, United States of America.
authors.forename: Lauren
authors.initials: L
authors.lastname: Kauffman
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, United States of America.
authors.forename: Veronica
authors.initials: V
authors.lastname: Balatti
authors.affiliation: Institute of Oncology Research, Bellinzona, Switzerland.
authors.forename: Luciano
authors.initials: L
authors.lastname: Cascione
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, United States of America.
authors.forename: Paolo
authors.initials: P
authors.lastname: Fadda
authors.affiliation: Department of Pathology, Ohio State Wexner Medical Center, Columbus, United States of America.
authors.forename: Frederick
authors.initials: F
authors.lastname: Racke
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, United States of America.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Santhanam
authors.affiliation: Department of Pathology, Ohio State Wexner Medical Center, Columbus, United States of America.
authors.forename: Stefan
authors.initials: S
authors.lastname: Costinean
mesh_terms: D000818:Animals; D001284:Atrophy / Q000378:metabolism; D016026:Bone Marrow Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs