title: Translocation of positively and negatively charged polystyrene nanoparticles in an in vitro placental model.
abstract: To obtain insight in translocation of nanoparticles across the placental barrier, translocation was studied for one positively and two negatively charged polystyrene nanoparticles (PS-NPs) of similar size in an in vitro model. The model consisted of BeWo b30 cells, derived from a human choriocarcinoma grown on a transwell insert forming a cell layer that separates an apical from a basolateral compartment. PS-NPs were characterized with respect to size, surface charge, morphology and protein corona. Translocation of PS-NPs was not related to PS-NP charge. Two PS-NPs were translocated across the BeWo transwell model to a lower extent than amoxicillin, a model compound known to be translocated over the placental barrier to only a limited extent, whereas one PS-NP showed a slightly higher translocation. Studies on the effect of transporter inhibitors on the translocation of the PS-NPs indicated that their translocation was not mediated by known transporters and mainly dependent on passive diffusion. It is concluded that the BeWo b30 model can be used as an efficient method to get an initial qualitative impression about the capacity of NPs to translocate across the placental barrier and set priorities in further in vivo studies on translocation of NPs to the fetus.
authors.affiliation: Department of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands. Electronic address: samantha.kloet@wur.nl.
authors.forename: Samantha K
authors.initials: SK
authors.lastname: Kloet
authors.affiliation: Department of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands; RIKILT - Wageningen UR, Wageningen, The Netherlands.
authors.forename: Agata P
authors.initials: AP
authors.lastname: Walczak
authors.affiliation: Department of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands.
authors.forename: Jochem
authors.initials: J
authors.lastname: Louisse
authors.affiliation: Department of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands.
authors.forename: Hans H J
authors.initials: HH
authors.lastname: van den Berg
authors.affiliation: RIKILT - Wageningen UR, Wageningen, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Bouwmeester
authors.affiliation: TNO, Department of Applied Environmental Sciences, Princetonlaan 6, 3584 CB Utrecht, The Netherlands.
authors.forename: Peter
authors.initials: P
authors.lastname: Tromp
authors.affiliation: Laboratory of Physical Chemistry and Colloid Science, Wageningen University, Wageningen, The Netherlands.
authors.forename: Remco G
authors.initials: RG
authors.lastname: Fokkink
authors.affiliation: Department of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands.
authors.forename: Ivonne M C M
authors.initials: IM
authors.lastname: Rietjens
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D053758:Nanoparticles / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D011137:Polystyrenes
keywords: BeWo b30 cells; Physico-chemical characteristics; Placental translocation; Protein corona