title: Association and Prognostic Significance of the Functional -1562C/T Polymorphism in the Promoter Region of MMP-9 in Turkish Patients with Gastric Cancer.
abstract: Matrix metalloproteinases (MMPs) are a group of zinc-dependent peptidases that participate in matrix turnover in solid malignancies. The aim of this study was twofold. First, we sought to investigate under a case-control design the association between the functional -1562C/T polymorphism in the promoter region of MMP-9 and gastric cancer (GC) in a Turkish sample. Second, we examined its prognostic significance in GC patients. A total of 144 subjects were enrolled in the case-control study (79 GC cases and 65 controls). Overall survival (OS) and progression-free survival (PFS) served as the main outcome measures in the longitudinal study. The MMP-9 -1562C/T polymorphism was genotyped using a polymerase chain reaction-restriction fragment length polymorphism method. The odds ratio (OR) of GC for the CC genotype relative to the CT+TT genotypes was not significant (OR = 0.89, 95 % confidence interval [CI] = 0.44-1.82, P = 0.75). These results did not change after allowance for age and sex in multivariable regression analysis (OR = 0.81, 95 % CI = 0.40-1.94, P = 0.84). When the MMP-9 -1562C/T polymorphism was analyzed among GC patients in relation to OS and PFS, we found no significant differences between subjects with the CC and CT+TT genotypes. In conclusion, the results of our study did not point toward a major role of the MMP-9 -1562C/T polymorphism in the pathogenesis and clinical course of GC in Turkish subjects.
authors.affiliation: Department of Medical Oncology, Ali Osman Sonmez Oncology Hospital, Bursa, Turkey, drniluferavci@hotmail.com.
authors.forename: Nilufer
authors.initials: N
authors.lastname: Avcı
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ture
authors.forename: Adem
authors.initials: A
authors.lastname: Deligonul
authors.forename: Erdem
authors.initials: E
authors.lastname: Cubukcu
authors.forename: Omer Fatih
authors.initials: OF
authors.lastname: Olmez
authors.forename: Serdar
authors.initials: S
authors.lastname: Sahinturk
authors.forename: Ali
authors.initials: A
authors.lastname: Topak
authors.forename: Ender
authors.initials: E
authors.lastname: Kurt
authors.forename: Turkkan
authors.initials: T
authors.lastname: Evrensel
authors.forename: Ahmet Bilgehan
authors.initials: AB
authors.lastname: Şahin
authors.forename: Tahsin
authors.initials: T
authors.lastname: Yakut
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D018572:Disease-Free Survival; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*; D014421:Turkey
chemical_list: C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9