title: DNA Aptamer Selected against Pancreatic Ductal Adenocarcinoma for in vivo Imaging and Clinical Tissue Recognition.
abstract: In this work, we have developed a truncated DNA aptamer, termed XQ-2d, with high affinity and specificity for pancreatic ductal adenocarcinoma (PDAC). Aptamer XQ-2d selectively binds to PL45 cells with a dissociation constant in the nanomolar range, as determined by its recognition of PL45 tumor cells in mice. Moreover, XQ-2d shows better recognition ratio for 40 tissue sections of clinical PDAC samples (82.5%) compared to the initial cell-SELEX selection library (5%). Therefore, XQ-2d can be considered a promising candidate as a tool for PDAC diagnosis and treatment.
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Wu
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Bai
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China ; 2. Departments of Chemistry, Department of Physiology and Functional Genomics, Center for Research at Bio/Nano Interface, Shands Cancer Center, University of Florida Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Fu
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China ; 3. College of Life and Environmental Science, National Navel Orange Engineering Research Center, Gannan Normal University, Economic & Technological Development Zone, Ganzhou 341000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Liu
authors.affiliation: 2. Departments of Chemistry, Department of Physiology and Functional Genomics, Center for Research at Bio/Nano Interface, Shands Cancer Center, University of Florida Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Carole
authors.initials: C
authors.lastname: Champanhac
authors.affiliation: 2. Departments of Chemistry, Department of Physiology and Functional Genomics, Center for Research at Bio/Nano Interface, Shands Cancer Center, University of Florida Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: I-Ting
authors.initials: IT
authors.lastname: Teng
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: 1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China ; 2. Departments of Chemistry, Department of Physiology and Functional Genomics, Center for Research at Bio/Nano Interface, Shands Cancer Center, University of Florida Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D000818:Animals; D052157:Aptamers, Nucleotide / Q000302:isolation & purification* / Q000378:metabolism*; D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis*; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D061848:Optical Imaging / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide
keywords: aptamer; cell-SELEX; molecular probe; pancreatic ductal adenocarcinoma