title: Characterization of Leishmania (Leishmania) waltoni n.sp. (Kinetoplastida: Trypanosomatidae), the Parasite Responsible for Diffuse Cutaneous Leishmaniasis in the Dominican Republic.
abstract: Leishmania parasites isolated, between 1979 and 1988 by the late Bryce Walton, from Dominican Republic (DR) patients with diffuse cutaneous leishmaniasis, were characterized using a panel of 12 isoenzymes, 23 monoclonal antibodies, small subunit ribosomal DNA (SSu rDNA), and multilocus sequence analysis (MLSA). The isoenzyme and monoclonal antibody profiles and the MLSA results showed that the Dominican Republic parasites were distinct from other described Leishmania species. This new species belongs to the mexicana complex, which is distributed in central and parts of northern South America. It is suggested that the parasites uniqueness from other members of the mexicana complex is related to it being isolated on an island for millions of years. If Leishmania (Leishmania) waltoni fails to adapt to some imported mammal, such as the house rat, it will be the only Leishmania to be classified as an endangered species. The excessive destruction of habitats on Hispaniola threatens the survival of its vectors and presumed natural reservoirs, such as the rodent hutias and the small insectivorous mammal solenodon. The concept of Leishmania species is discussed in the light of recent evaluations on criteria for defining bacterial species.
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England jayusp@hotmail.com parasito@univ-montp1.fr.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Shaw
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England.
authors.forename: Francine
authors.initials: F
authors.lastname: Pratlong
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England.
authors.forename: Lucile
authors.initials: L
authors.lastname: Floeter-Winter
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England.
authors.forename: Edna
authors.initials: E
authors.lastname: Ishikawa
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England.
authors.forename: Fouad
authors.initials: F
authors.lastname: El Baidouri
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England.
authors.forename: Christophe
authors.initials: C
authors.lastname: Ravel
authors.affiliation: Parasitology Department, Biomedical Sciences Institute, São Paulo University, São Paulo, Brazil; French National Reference Centre on Leishmaniasis, Montpellier University, Montpellier, France; Biology Department, BioSciences Institute, São Paulo University, São Paulo, Brazil; Tropical Medicine Nucleus, Pará Federal University, Brazil; School of Life Sciences, University of Lincoln, Lincoln, England jayusp@hotmail.com parasito@univ-montp1.fr.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Dedet
mesh_terms: D000913:Antibodies, Protozoan / Q000276:immunology; D001483:Base Sequence; D004293:Dominican Republic / Q000453:epidemiology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007891:Leishmania / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D016774:Leishmaniasis, Diffuse Cutaneous / Q000453:epidemiology / Q000469:parasitology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D000913:Antibodies, Protozoan; D007527:Isoenzymes