title: Use of Nonradioactive Detection Method for North- and South-Western Blot.
abstract: Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here we describe the use of a North-western and South-western blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
authors.affiliation: Institut für Immunologie, Carl Gustav Carus Technische Universität Dresden, Germany, Fetscherstrasse 74, Dresden, 01307, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Franke
authors.forename: Daniel
authors.initials: D
authors.lastname: Gräfe
authors.forename: Holger
authors.initials: H
authors.lastname: Bartsch
authors.forename: Michael P
authors.initials: MP
authors.lastname: Bachmann
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000379:methods*; D026501:Blotting, Southwestern / Q000379:methods*; D015153:Blotting, Western / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D015342:DNA Probes / Q000378:metabolism; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D012313:RNA; D004247:DNA; D008780:Methyltransferases