title: Preparation and Characterization of Naringenin-Loaded Elastic Liposomes for Topical Application.
abstract: Excessive production of radical oxygen species in skin is a contributor to a variety of skin pathologies. Naringenin is a potent antioxidant. The purpose of the present study was to develop elastic liposomes for naringenin topical application. Naringenin-loaded elastic liposomes containing different amounts of Tween 80 and cholesterol were prepared. The physicochemical properties including vesicle size, surface charge, encapsulation efficiency, and permeability capacity were determined to evaluate the effect of components. The stability of formulation and skin irritation caused by drug-loaded elastic liposomes were also evaluated for assessment of the clinical utility of elastic liposomes. Saturated aqueous solution of naringenin and naringenin dissolved in 10% Tween 80 solution (5 mg/mL) were used as the control group. The result showed that in using elastic liposomes as carrier, the deposition amounts in the skin of naringenin were significantly increased about 7.3~11.8-fold and 1.2~1.9-fold respectively, when compared with the saturated aqueous solution and Tween 80 solution-treated groups. The level of drug was more than 98.89±3.90% after 3 months of storage at 4℃. In a skin irritation test, the result showed experimental formulation exhibit considerably less irritating than the positive control (paraformaldehyde-treated) group, suggesting its potential therapeutic application.
authors.affiliation: Department of Neurology, China Medical University Hospital, Taichung, Taiwan, ROC; School of Medicine, Medical College, China Medical University, Taichung, Taiwan, ROC.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Tsai
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Yaw-Bin
authors.initials: YB
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Jhih-Wun
authors.initials: JW
authors.lastname: Fang
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Yaw-Syan
authors.initials: YS
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Pao-Chu
authors.initials: PC
authors.lastname: Wu
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D004339:Drug Compounding; D004355:Drug Stability; D004548:Elasticity; D044950:Flavanones / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D008081:Liposomes / Q000737:chemistry; D010539:Permeability; D011136:Polysorbates / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D012882:Skin Tests
chemical_list: D000975:Antioxidants; D044950:Flavanones; D008081:Liposomes; D011136:Polysorbates; C005273:naringenin