title: Enhancement of enzymatic saccharification of corn stover with sequential Fenton pretreatment and dilute NaOH extraction.
abstract: In this study, an effective method by the sequential Fenton pretreatment and dilute NaOH extraction (FT-AE) was chosen for pretreating corn stover. Before dilute NaOH (0.75 wt%) extraction at 90 °C for 1h, Fenton reagent (0.95 g/L of FeSO4 and 29.8 g/L of H2O2) was employed to pretreat CS at a solid/liquid ratio of 1/20 (w/w) at 35 °C for 30 min. The changes in the cellulose structural characteristics (porosity, morphology, and crystallinity) of the pretreated solid residue were correlated with the enhancement of enzymatic saccharification. After being enzymatically hydrolyzed for 72 h, the reducing sugars and glucose from the hydrolysis of 60 g/L FT-AE-CS pretreated could be obtained at 40.96 and 23.61 g/L, respectively. Finally, the recovered hydrolyzates containing glucose had no inhibitory effects on the ethanol fermenting microorganism. In conclusion, the sequential Fenton pretreatment and dilute NaOH extraction has high potential application in future.
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Xue
authors.affiliation: Department of Biological Systems Engineering, Bioproducts, Sciences and Engineering Laboratory, Washington State University, Richland, WA 99354, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Zheng-Zhong
authors.initials: ZZ
authors.lastname: Zhu
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Qing
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D007501:Iron / Q000494:pharmacology*; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013997:Time Factors; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
chemical_list: C045076:Fenton's reagent; D000431:Ethanol; D012972:Sodium Hydroxide; D006861:Hydrogen Peroxide; D007501:Iron; D002480:Cellulase; D005947:Glucose
keywords: Combination pretreatment; Corn stover; Fenton reagent; NaOH extraction; Saccharification