title: Protective immune-response of aluminium hydroxide gel adjuvanted phage lysate of Brucella abortus S19 in mice against direct virulent challenge with B. abortus 544.
abstract: The prophylactic efficacies of plain and alum adsorbed lysate were evaluated by direct virulent challenge in mice model. A recently isolated brucellaphage 'ϕLd' was used for generation of lysates. Twenty four h incubated Brucella abortus S19 broth cultures standardized to contain approximately 10(8) CFU/ml were found suitable for generation of lysates. Three lysate batches produced through separate cycles did not show any significant variation with respect to protein and polysaccharide contents, endotoxin level and phage counts, indicating that compositionally stable lysate preparations can be generated through an optimized production process. Three polypeptides of ∼16, 19 and 23 kDa could be identified as immuno-dominant antigens of the lysate which induced both humoral and cell-mediated immune responses in a dose dependent manner. Results of efficacy evaluation trial confirmed dose-dependent protective potencies of lysate preparation. The lysate with an antigenic dose of 0.52 μg protein and 60 μg CHO adsorbed on aluminium gel (0.1 percent aluminium concentration) exhibited the highest protective potency which was greater than that induced by standard S19 vaccine. Phage lysate methodology provides a very viable option through which an improved immunizing preparation with all desirable traits can be developed against brucellosis, and integrated with immunization programmes in a more efficient manner.
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Lata
authors.initials: L
authors.lastname: Jain
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP 243122, India. Electronic address: mayankvet9@gmail.com.
authors.forename: Mayank
authors.initials: M
authors.lastname: Rawat
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Awadhesh
authors.initials: A
authors.lastname: Prajapati
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Tiwari
authors.affiliation: Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Bablu
authors.initials: B
authors.lastname: Kumar
authors.affiliation: Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Chaturvedi
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Guru Angad Dev Veterinary and Animal Science University, Ludhiana, Punjab, India.
authors.forename: H M
authors.initials: HM
authors.lastname: Saxena
authors.affiliation: Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Sarvanan
authors.initials: S
authors.lastname: Ramakrishnan
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Jatin
authors.initials: J
authors.lastname: Kumar
authors.affiliation: Department of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, UP 243122, India.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Kerketta
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000536:Aluminum Hydroxide / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001435:Bacteriophages; D002003:Brucella abortus / Q000276:immunology* / Q000472:pathogenicity / Q000821:virology; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005782:Gels; D007111:Immunity, Cellular; D051379:Mice; D014774:Virulence
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D016207:Cytokines; D005782:Gels; D000536:Aluminum Hydroxide
keywords: Brucellosis; Cell mediate immune response; Humoral response; Phage lysate; Protective efficacy