title: Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene.
abstract: GRP is a vitamin K-dependent protein with orthologs in all vertebrate taxonomic groups and two paralogs in teleosts. However, no data is available about GRP transcriptional gene regulation. We report a functional promoter for zebrafish grp2 gene regulated by Sox9b, Sox10, Ets1 and Mef2ca as determined by in vitro assays. This was confirmed in vivo for Sox9b and Sox10. Due to the high conservation between human GRP and grp2, its zebrafish ortholog, our results are relevant for the study of human GRP gene regulation and provide new insights towards understanding GRP function.
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal; PhD Program in Biomedical Sciences, University of Algarve, Faro, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Fazenda
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal; Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal. Electronic address: nconcei@ualg.pt.
authors.forename: N
authors.initials: N
authors.lastname: Conceição
authors.affiliation: Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal; Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal. Electronic address: lcancela@ualg.pt.
authors.forename: M L
authors.initials: ML
authors.lastname: Cancela
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D064326:MEF2 Transcription Factors / Q000235:genetics / Q000378:metabolism; D009990:Oryzias / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional; D055747:SOX Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D055757:SOXE Transcription Factors / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D015533:Transcriptional Activation; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D064326:MEF2 Transcription Factors; C588168:Mef2ca protein, zebrafish; D011506:Proteins; D050783:Proto-Oncogene Proteins c-ets; D055747:SOX Transcription Factors; D055758:SOX9 Transcription Factor; D055757:SOXE Transcription Factors; C504919:Sox9b protein, zebrafish; C528959:Ucma protein, human; D029961:Zebrafish Proteins; C442229:sox10 protein, zebrafish
keywords: Gene regulation; Gla-rich protein; Sox family; Transcription factors