title: Effects of hypergravity on adipose-derived stem cell morphology, mechanical property and proliferation.
abstract: Alteration in specific inertial conditions can lead to changes in morphology, proliferation, mechanical properties and cytoskeleton of cells. In this report, the effects of hypergravity on morphology of Adipose-Derived Stem Cells (ADSCs) are indicated. ADSCs were repeatedly exposed to discontinuous hypergravity conditions of 10 g, 20 g, 40 g and 60 g by utilizing centrifuge (three times of 20 min exposure, with an interval of 40 min at 1 g). Cell morphology in terms of length, width and cell elongation index and cytoskeleton of actin filaments and microtubules were analyzed by image processing. Consistent changes observed in cell elongation index as morphological change. Moreover, cell proliferation was assessed and mechanical properties of cells in case of elastic modulus of cells were evaluated by Atomic Force Microscopy. Increase in proliferation and decrease in elastic modulus of cells are further results of this study. Staining ADSC was done to show changes in cytoskeleton of the cells associated to hypergravity condition specifically in microfilament and microtubule components. After exposing to hypergravity, significant changes were observed in microfilaments and microtubule density as components of cytoskeleton. It was concluded that there could be a relationship between changes in morphology and MFs as the main component of the cells.
authors.affiliation: Medical Nanotechnology and Tissue Engineering Research Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Tavakolinejad
authors.affiliation: Department of Biomedical Engineering, University of Isfahan, Isfahan, Iran. Electronic address: m.rabbani@eng.ui.ac.ir.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Rabbani
authors.affiliation: Medical Nanotechnology and Tissue Engineering Research Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Janmaleki
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D018471:Hypergravity; D018625:Microscopy, Atomic Force; D013234:Stem Cells / Q000166:cytology*
keywords: Adipose derived stem cells; Cell morphology; Cytoskeleton; Hypergravity; Mechanical property; Proliferation