title: Protective efficacy of adenovirus/protein vaccines against SIV challenges in rhesus monkeys.
abstract: Preclinical studies of viral vector-based HIV-1 vaccine candidates have previously shown partial protection against neutralization-resistant virus challenges in rhesus monkeys. In this study, we evaluated the protective efficacy of adenovirus serotype 26 (Ad26) vector priming followed by purified envelope (Env) glycoprotein boosting. Rhesus monkeys primed with Ad26 vectors expressing SIVsmE543 Env, Gag, and Pol and boosted with AS01B-adjuvanted SIVmac32H Env gp140 demonstrated complete protection in 50% of vaccinated animals against a series of repeated, heterologous, intrarectal SIVmac251 challenges that infected all controls. Protective efficacy correlated with the functionality of Env-specific antibody responses. Comparable protection was also observed with a similar Ad/Env vaccine against repeated, heterologous, intrarectal SHIV-SF162P3 challenges. These data demonstrate robust protection by Ad/Env vaccines against acquisition of neutralization-resistant virus challenges in rhesus monkeys.
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA 02139, USA. dbarouch@bidmc.harvard.edu.
authors.forename: Dan H
authors.initials: DH
authors.lastname: Barouch
authors.affiliation: Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA 02139, USA.
authors.forename: Galit
authors.initials: G
authors.lastname: Alter
authors.affiliation: Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA 02139, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Broge
authors.affiliation: Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA 02139, USA.
authors.forename: Caitlyn
authors.initials: C
authors.lastname: Linde
authors.affiliation: Thayer School of Engineering at Dartmouth, Hanover, NH 03755, USA.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Ackerman
authors.affiliation: Thayer School of Engineering at Dartmouth, Hanover, NH 03755, USA.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Brown
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Erica N
authors.initials: EN
authors.lastname: Borducchi
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Kaitlin M
authors.initials: KM
authors.lastname: Smith
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Nkolola
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Shields
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Lily
authors.initials: L
authors.lastname: Parenteau
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: James B
authors.initials: JB
authors.lastname: Whitney
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Abbink
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Ng'ang'a
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Seaman
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Christy L
authors.initials: CL
authors.lastname: Lavine
authors.affiliation: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Perry
authors.affiliation: University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.affiliation: New England Primate Research Center, Southborough, MA 01772, USA.
authors.forename: Arnaud D
authors.initials: AD
authors.lastname: Colantonio
authors.affiliation: Bioqual, Rockville, MD 20852, USA.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Lewis
authors.affiliation: Children's Hospital, Boston, MA 02115, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: JPT Peptide Technologies GmbH, 12489 Berlin, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Wenschuh
authors.affiliation: JPT Peptide Technologies GmbH, 12489 Berlin, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Reimer
authors.affiliation: AIDS and Cancer Virus Program, Leidos Biomedical Research, Frederick National Laboratory, Frederick, MD 21702, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Piatak
authors.affiliation: AIDS and Cancer Virus Program, Leidos Biomedical Research, Frederick National Laboratory, Frederick, MD 21702, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Lifson
authors.affiliation: Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Handley
authors.affiliation: Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Herbert W
authors.initials: HW
authors.lastname: Virgin
authors.affiliation: GSK Vaccines, 1330 Rixensart, Belgium.
authors.forename: Marguerite
authors.initials: M
authors.lastname: Koutsoukos
authors.affiliation: GSK Vaccines, 1330 Rixensart, Belgium.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Lorin
authors.affiliation: GSK Vaccines, 1330 Rixensart, Belgium.
authors.forename: Gerald
authors.initials: G
authors.lastname: Voss
authors.affiliation: Janssen Infectious Diseases and Vaccines (formerly Crucell), 2301 Leiden, Netherlands.
authors.forename: Mo
authors.initials: M
authors.lastname: Weijtens
authors.affiliation: Janssen Infectious Diseases and Vaccines (formerly Crucell), 2301 Leiden, Netherlands.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Pau
authors.affiliation: Janssen Infectious Diseases and Vaccines (formerly Crucell), 2301 Leiden, Netherlands.
authors.forename: Hanneke
authors.initials: H
authors.lastname: Schuitemaker
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D062705:Adenovirus Vaccines / Q000276:immunology*; D019264:Adoptive Transfer; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology; D015684:Gene Products, pol / Q000276:immunology; D005822:Genetic Vectors / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology; D007117:Immunization, Secondary; D008253:Macaca mulatta; D008297:Male; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D062705:Adenovirus Vaccines; D057134:Antibodies, Neutralizing; D015686:Gene Products, env; D015683:Gene Products, gag; D015684:Gene Products, pol; D015395:Histocompatibility Antigens Class I; D018449:SAIDS Vaccines