title: Nitroreductase-triggered activation of a novel caged fluorescent probe obtained from methylene blue.
abstract: A near-infrared fluorescent probe based on methylene blue (p-NBMB) was developed for the detection of nitroreductase. Conjugating methylene blue with a p-nitrobenzyl moiety enables it to be activated by nitroreductase-catalyzed 1,6-elimination, resulting in the release of an active methylene blue fluorophore.
authors.affiliation: Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. seongjo@olemiss.edu.
authors.forename: Jungeun
authors.initials: J
authors.lastname: Bae
authors.forename: Louis E
authors.initials: LE
authors.lastname: McNamara
authors.forename: Manal A
authors.initials: MA
authors.lastname: Nael
authors.forename: Fakhri
authors.initials: F
authors.lastname: Mahdi
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Doerksen
authors.forename: Gene L
authors.initials: GL
authors.lastname: Bidwell
authors.forename: Nathan I
authors.initials: NI
authors.lastname: Hammer
authors.forename: Seongbong
authors.initials: S
authors.lastname: Jo
mesh_terms: D015195:Drug Design; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008751:Methylene Blue / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009601:Nitroreductases / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D005456:Fluorescent Dyes; C000607553:p-nitrobenzyl 3,7-bis(dimethylamino)-10H-phenothiazine-10-carboxylate; D009601:Nitroreductases; D008751:Methylene Blue