title: MUC1-Targeted Cancer Cell Photothermal Ablation Using Bioinspired Gold Nanorods.
abstract: Recent studies have highlighted the overexpression of mucin 1 (MUC1) in various epithelial carcinomas and its role in tumorigenesis. These mucins present a novel targeting opportunity for nanoparticle-mediated photothermal cancer treatments due to their unique antenna-like extracellular extension. In this study, MUC1 antibodies and albumin were immobilized onto the surface of gold nanorods using a "primer" of polydopamine (PD), a molecular mimic of catechol- and amine-rich mussel adhesive proteins. PD forms an adhesive platform for the deposition of albumin and MUC1 antibodies, achieving a surface that is stable, bioinert and biofunctional. Two-photon luminescence confocal and darkfield scattering imaging revealed targeting of MUC1-BSA-PD-NRs to MUC1+ MCF-7 breast cancer and SCC-15 squamous cell carcinoma cells lines. Treated cells were exposed to a laser encompassing the near-infrared AuNR longitudinal surface plasmon and assessed for photothermal ablation. MUC1-BSA-PD-NRs substantially decreased cell viability in photoirradiated MCF-7 cell lines vs. MUC1- MDA-MB-231 breast cancer cells (p < 0.005). Agents exhibited no cytotoxicity in the absence of photothermal treatment. The facile nature of the coating method, combined with targeting and photoablation efficacy, are attractive features of these candidate cancer nanotherapeutics.
authors.affiliation: Department of Biomedical Engineering, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America; Department of Bioengineering, University of California, Berkeley, Berkeley, California, United States of America.
authors.forename: Daria C
authors.initials: DC
authors.lastname: Zelasko-Leon
authors.affiliation: Department of Biomedical Engineering, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America; Department of Bioengineering, University of California, Berkeley, Berkeley, California, United States of America.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Fuentes
authors.affiliation: Department of Biomedical Engineering, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America; Department of Bioengineering, University of California, Berkeley, Berkeley, California, United States of America; Department of Materials Science and Engineering, University of California, Berkeley, Berkeley, California, United States of America.
authors.forename: Phillip B
authors.initials: PB
authors.lastname: Messersmith
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D018396:Mucin-1 / Q000378:metabolism*; D043942:Nanotubes; D010789:Phototherapy; D011108:Polymers / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D007211:Indoles; C060192:MUC1 protein, human; D018396:Mucin-1; D011108:Polymers; C568283:polydopamine; D012710:Serum Albumin, Bovine; D006046:Gold