title: Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.
abstract: In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2|Ratio| ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.
authors.affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Xin-Zheng
authors.initials: XZ
authors.lastname: Huang
authors.affiliation: Institute of Agro-food Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Jie-Yin
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Xiao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Xiao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China.
authors.forename: Jun-Xiang
authors.initials: JX
authors.lastname: Wu
authors.affiliation: Department of Biological Chemistry, Rothamsted Research, Harpenden, AL5 2JQ, UK.
authors.forename: Jing-Jiang
authors.initials: JJ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D003368:Gossypium / Q000737:chemistry / Q000378:metabolism*; D060434:Herbivory; D007814:Larva / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D013729:Terpenes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D010937:Plant Growth Regulators; D013729:Terpenes; D014157:Transcription Factors; D055549:Volatile Organic Compounds