title: Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction.
abstract: We develop a ligation-mediated polymerase chain reaction (PCR) for real-time detection of small biological molecules in a high-throughput format. This method is extremely sensitive with a detection limit of as low as 18.8 fM for ATP and 17.3 fM for NAD(+), and it can discriminate target molecules from their analogues as well.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis* / Q000378:metabolism; D057230:Limit of Detection; D009243:NAD / Q000032:analysis* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D009243:NAD; D000244:Adenosine Diphosphate