title: Defensive Armor of Potato Tubers: Nonpolar Metabolite Profiling, Antioxidant Assessment, and Solid-State NMR Compositional Analysis of Suberin-Enriched Wound-Healing Tissues.
abstract: The cultivation, storage, and distribution of potato tubers are compromised by mechanical damage and suboptimal healing. To investigate wound-healing progress in cultivars with contrasting russeting patterns, metabolite profiles reported previously for polar tissue extracts were complemented by GC/MS measurements for nonpolar extracts and quantitative (13)C NMR of interfacial solid suspensions. Potential marker compounds that distinguish cultivar type and wound-healing time point included fatty acids, fatty alcohols, alkanes, glyceryl esters, α,ω-fatty diacids, and hydroxyfatty acids. The abundant long-chain fatty acids in nonpolar extracts and solids from the smooth-skinned Yukon Gold cultivar suggested extensive suberin biopolymer formation; this hypothesis was supported by high proportions of arenes, alkenes, and carbonyl groups in the solid and among the polar markers. The absence of many potential marker classes in nonpolar Atlantic extracts and interfacial solids suggested a limited extent of suberization. Modest scavenging activities of all nonpolar extracts indicate that the majority of antioxidants produced in response to wounding are polar.
authors.affiliation: †Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies, New York, New York 10031, United States.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Dastmalchi
authors.affiliation: †Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies, New York, New York 10031, United States.
authors.forename: Linda
authors.initials: L
authors.lastname: Kallash
authors.affiliation: †Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies, New York, New York 10031, United States.
authors.forename: Isabel
authors.initials: I
authors.lastname: Wang
authors.affiliation: ̂Department of Natural Sciences, Hostos Community College, 500 Grand Concourse, Bronx, New York 10451, United States.
authors.forename: Van C
authors.initials: VC
authors.lastname: Phan
authors.affiliation: †Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies, New York, New York 10031, United States.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Huang
authors.affiliation: ‡Laboratori del Suro, Departament de Biologia, Facultat de Ciències, University of Girona, Campus Montilivi s/n, Girona, E-17071 Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Serra
authors.affiliation: †Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies, New York, New York 10031, United States.
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Stark
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D008055:Lipids / Q000032:analysis* / Q000096:biosynthesis; D009682:Magnetic Resonance Spectroscopy; D035281:Plant Tubers / Q000737:chemistry* / Q000502:physiology; D011198:Solanum tuberosum / Q000737:chemistry / Q000145:classification / Q000502:physiology*; D014945:Wound Healing
chemical_list: D000975:Antioxidants; D008055:Lipids; C065875:suberin
keywords: ABTS•+ scavenging activity; GC/MS; Solanum tuberosum; closing layer; potential markers; solid-state NMR; suberin; wound periderm