title: [Isolation and gene screening for collagen phagocytic subpopulation of fibroblasts and non-collagen  phagocytic subpopulation of fibroblasts].
abstract: OBJECTIVE
  To isolate the collagen phagocytic subpopulation of fibroblast (CPSF) and non-collagen phagocytic subpopulation of fibroblast (nCPSF) and to identify their differentially expressed genes.
  
  
  METHODS
  The CPSF and nCPSF was isolated by using collagen-fluorescein-isothiocynate-latex bead (COL-FITC-LB) phagocytosis technique and FCM sorting method. Microarray analysis was used to screen the differentially expressed genes, which were verified by real-time PCR.
  
  
  RESULTS
  CPSF and nCPSF was successfully isolated. Seventeen differentially expressed genes were identified. Compared with nCPSF, the expression of 12 or 5 genes was up-regulated or down-regulated in CPSF. Three of the 12 up-regulated genes were urokinase plasminogen activator receptor-associated protein (uPARAP), cytochrome b-245, beta polypeptide (CYBB) and Hook homolog 1 (HOOK1), which were confirmed by real-time PCR. uPARAP mRNA expression level in CPSF was 2788 times of that in nCPSF. CYBB mRNA expression in CPSF was only 0.85 times of that in nCPSF. HOOK1 mRNA expression in CPSF was 1.96 times of that in nCPSF (P<0.05).
  
  
  CONCLUSIONS
  A novel method is successfully established to isolate CPSF and nCPSF. uPARAP is the main differentially expressed gene in CPSF and nCPSF, which is obviously involved in the fibroblast collagen phagocytosis. It might be a potential biomarker for treatment of collagen diseases.
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Endodontics, Beijing Stomatological Hospital, Capital Medical University, BeiJing 100050, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Su
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Xinchun
authors.initials: X
authors.lastname: Jian
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Cong
authors.initials: C
authors.lastname: Mu
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Changyun
authors.initials: C
authors.lastname: Fang
mesh_terms: D003094:Collagen / Q000235:genetics*; D015536:Down-Regulation; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D046228:Microarray Analysis; D010587:Phagocytosis; D015854:Up-Regulation
chemical_list: D003094:Collagen