title: Different chronological patterns of appearance of blood derived milk components during mastitis indicate different mechanisms of transfer from blood into milk.
abstract: This study aimed to describe chronological patterns of changes of various candidate blood components in milk during the acute phase of a mammary immune response in detail. Eight dairy cows were challenged with Escherichia coli lipopolysaccharide in one udder quarter. Milk from challenged and control quarters and blood samples were taken before, and 1 and 2 h after challenge and then every 15 min until 5 h after challenge. The SCC, serum albumin, immunoglobulin (Ig)G1, IgG2, lactate dehydrogenase (LDH), and L-lactate in milk and blood, and α-lactalbumin in blood were analysed. All selected parameters in milk increased in challenged quarters but did not increase in control quarters. Milk IgG1, IgG2, serum albumin, and LDH were already significantly increased at 2 h after challenge whereas a significant increase of SCC was detectable at 2.75 h and L-lactate was increased at 2.25 h after challenge. In blood L-lactate was increased at 3.75 h after challenge, however, other factors in blood did not change significantly within the 5 h of experiment. In conclusion, the increase of blood components in milk during inflammation follows two different patterns: There is a rapid increase for IgG1, IgG2, or LDH, before the increase of SCC, and their concentrations reach a plateau within 3 h. On the other hand, SCC and L-lactate show a slower but consistent increase not reaching a plateau within 5 h after LPS challenge. L-lactate increases to higher concentrations in milk than in blood. This clearly shows that the increase of blood components follows different patterns and is therefore a controlled and compound-specific process and not exclusively an unspecific type of leakage.
authors.affiliation: Vetsuisse Faculty University of Zurich,Clinic of Reproductive Medicine,Switzerland.
authors.forename: Olga
authors.initials: O
authors.lastname: Wellnitz
authors.affiliation: Vetsuisse Faculty University of Bern,Veterinary Physiology,Switzerland.
authors.forename: Christina
authors.initials: C
authors.lastname: Zbinden
authors.affiliation: Vetsuisse Faculty University of Zurich,Clinic of Reproductive Medicine,Switzerland.
authors.forename: Johannes
authors.initials: J
authors.lastname: Lüttgenau
authors.affiliation: Vetsuisse Faculty University of Zurich,Clinic of Reproductive Medicine,Switzerland.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Bollwein
authors.affiliation: Vetsuisse Faculty University of Bern,Veterinary Physiology,Switzerland.
authors.forename: Rupert M
authors.initials: RM
authors.lastname: Bruckmaier
mesh_terms: D000210:Acute-Phase Reaction / Q000097:blood / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002452:Cell Count; D004926:Escherichia coli; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000097:blood; D007768:Lactalbumin / Q000097:blood; D019344:Lactic Acid / Q000032:analysis / Q000097:blood; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000276:immunology; D008414:Mastitis, Bovine / Q000097:blood* / Q000378:metabolism*; D008892:Milk / Q000737:chemistry* / Q000166:cytology; D012709:Serum Albumin / Q000032:analysis; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D012709:Serum Albumin; D019344:Lactic Acid; D007768:Lactalbumin; D007770:L-Lactate Dehydrogenase
keywords: blood-milk barrier; dairy cow; mastitis