title: Gene expression of estrogen receptor-alpha in orbital fibroblasts in Graves' ophthalmopathy.
abstract: Graves' ophthalmopathy (GO) is one of the most severe clinical manifestations of Graves' disease (GD), and its treatment might involve high-dose glucocorticoid therapy. The higher incidence of GO among females, and the reported association between polymorphisms of estrogen receptor (ER) and GD susceptibility have led us to question the role of estrogen and its receptor in GO pathogenesis. We, thus, assessed estrogen receptor-alpha (ERA) gene expression in cultures of orbital fibroblasts from a patient with GO before (controls) and after treatment with 10 nM and 100 nM dexamethasone (DEX). Orbital fibroblasts showed ERA gene expression. In the cells treated with 10 nM and 100 nM DEX, ERA gene expression was, respectively, 85% higher and 74% lower, than in the control group. We concluded that ERA gene expression is found in the orbital fibroblasts of patient with GO, which may be affected by glucocorticoids in a dose-related manner. Arch Endocrinol Metab. 2015;59(3):273-6.
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Sarah Santiloni
authors.initials: SS
authors.lastname: Cury
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Miriane
authors.initials: M
authors.lastname: Oliveira
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Síbio
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Sueli
authors.initials: S
authors.lastname: Clara
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Renata De Azevedo Melo
authors.initials: Rde A
authors.lastname: Luvizotto
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Sandro
authors.initials: S
authors.lastname: Conde
authors.affiliation: Unesp, Botucatu, SP, Brazil.
authors.forename: Edson Nacib
authors.initials: EN
authors.lastname: Jorge
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Vania Dos Santos
authors.initials: Vdos S
authors.lastname: Nunes
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Célia Regina
authors.initials: CR
authors.lastname: Nogueira
authors.affiliation: Internal Medicine Department, Botucatu Medical School, Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Gláucia Maria Ferreira da Silva
authors.initials: GM
authors.lastname: Mazeto
mesh_terms: D002478:Cells, Cultured; D003907:Dexamethasone / Q000627:therapeutic use; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005938:Glucocorticoids / Q000627:therapeutic use; D049970:Graves Ophthalmopathy / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D005938:Glucocorticoids; D003907:Dexamethasone