title: Genetic determinants of pathogenicity of Escherichia coli isolated from children with acute diarrhea in Maputo, Mozambique.
abstract: BACKGROUND
  Diarrheagenic Escherichia coli (DEC) represents one of the leading cause of diarrhoea in developing countries. In this study a molecular approach was applied for the detection of diarrheagenic Escherichia coli (DEC) circulating in Maputo, Mozambique.
  
  
  METHODS
  All isolates were PCR tested for species-specific genes and for 11 molecular markers: stx1, stx2, eae, bfpA, lt, st, ipaH, aap, aggR CVD432 and daaE.
  
  
  RESULTS
  Of the 80 E. coli isolated, 74% were potential DEC: 21% EIEC, 19% EPEC, 15% EAEC, 13% ETEC, 5% DAEC and 1% hybrids.
  
  
  CONCLUSIONS
  This study revealed the complexity of the etiology of diarrhea caused by pathogenic E. coli in Mozambique, and the risk of the emergence of new pathogenic variants due to the horizontal transmission of pathogenicity factors.
authors.affiliation: Faculdade de Ciências, UEM, Moçambique. sumbanaj@yahoo.com.br.
authors.forename: Jose
authors.initials: J
authors.lastname: Sumbana
authors.forename: Elisa
authors.initials: E
authors.lastname: Taviani
authors.forename: Alice
authors.initials: A
authors.lastname: Manjate
authors.forename: Bianca
authors.initials: B
authors.lastname: Paglietti
authors.forename: Antonella
authors.initials: A
authors.lastname: Santona
authors.forename: Mauro M
authors.initials: MM
authors.lastname: Colombo
mesh_terms: D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D009073:Mozambique / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors