title: All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation.
abstract: We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain.
authors.affiliation: National University of Singapore , Yong Loo Lin School of Medicine, Department of Physiology, Singapore 117597, Singapore ; Nanyang Technological University , Lee Kong Chian School of Medicine, Proteos, Biopolis, Level 4, 61 Biopolis Drive, #04-06/07, Singapore 138673, Singapore ; Institute of Molecular and Cell Biology , ASTAR, Proteos, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore ; Marine Biological Laboratory , 7 MBL Street, Woods Hole, Massachusetts 02543, United States.
authors.forename: Shun Qiang
authors.initials: SQ
authors.lastname: Lo
authors.affiliation: National University of Singapore , Graduate School of Integrative Sciences and Engineering, Singapore 117456, Singapore ; National University of Singapore , Yong Loo Lin School of Medicine, Department of Pharmacology, Singapore 117599, Singapore ; Singapore Institute of Clinical Sciences (SICS) , ASTAR, Brenner Centre for Molecular Medicine, 30 Medical Drive, Singapore 117609, Singapore.
authors.forename: Dawn X P
authors.initials: DX
authors.lastname: Koh
authors.affiliation: National University of Singapore , Yong Loo Lin School of Medicine, Department of Pharmacology, Singapore 117599, Singapore ; Singapore Institute of Clinical Sciences (SICS) , ASTAR, Brenner Centre for Molecular Medicine, 30 Medical Drive, Singapore 117609, Singapore.
authors.forename: Judy C G
authors.initials: JC
authors.lastname: Sng
authors.affiliation: National University of Singapore , Yong Loo Lin School of Medicine, Department of Physiology, Singapore 117597, Singapore ; Nanyang Technological University , Lee Kong Chian School of Medicine, Proteos, Biopolis, Level 4, 61 Biopolis Drive, #04-06/07, Singapore 138673, Singapore ; Institute of Molecular and Cell Biology , ASTAR, Proteos, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore ; Marine Biological Laboratory , 7 MBL Street, Woods Hole, Massachusetts 02543, United States ; Korea Institute of Science and Technology , Center for Functional Connectomics, 39-1 Hawolgokdong, Seongbukgu, Seoul 136-791, Republic of Korea.
authors.forename: George J
authors.initials: GJ
authors.lastname: Augustine
keywords: barrel cortex; channelrhodopsin; optogenetics; voltage-sensitive dye