title: Mitochondrial DNA Haplogroup A Decreases the Risk of Drug Addiction but Conversely Increases the Risk of HIV-1 Infection in Chinese Addicts.
abstract: Drug addiction is one of the most serious social problems in the world today and addicts are always at a high risk of acquiring HIV infection. Mitochondrial impairment has been reported in both drug addicts and in HIV patients undergoing treatment. In this study, we aimed to investigate whether mitochondrial DNA (mtDNA) haplogroup could affect the risk of drug addiction and HIV-1 infection in Chinese. We analyzed mtDNA sequence variations of 577 Chinese intravenous drug addicts (289 with HIV-1 infection and 288 without) and compared with 2 control populations (n = 362 and n = 850). We quantified the viral load in HIV-1-infected patients with and without haplogroup A status and investigated the potential effect of haplogroup A defining variants m.4824A > G and m.8794C > T on the cellular reactive oxygen species (ROS) levels by using an allotopic expression assay. mtDNA haplogroup A had a protective effect against drug addiction but appeared to confer an increased risk of HIV infection in addicts. HIV-1-infected addicts with haplogroup A had a trend for a higher viral load, although the mean viral load was similar between carriers of haplogroup A and those with other haplogroup. Hela cells overexpressing allele m.8794 T showed significantly decreased ROS levels as compared to cells with the allele m.8794C (P = 0.03). Our results suggested that mtDNA haplogroup A might protect against drug addiction but increase the risk of HIV-1 infection. The contradictory role of haplogroup A might be caused by an alteration in mitochondrial function due to a particular mtDNA ancestral variant.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China.
authors.forename: A-Mei
authors.initials: AM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China.
authors.forename: Qiu-Xiang
authors.initials: QX
authors.lastname: Hu
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China.
authors.forename: Feng-Liang
authors.initials: FL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Bi
authors.affiliation: Department of Laboratory Medicine Science, Dehong Prefecture People's Hospital of Yunnan Province, Yunnan, China.
authors.forename: Bi-Qing
authors.initials: BQ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China.|Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.affiliation: Exmouth, Devon, UK.
authors.forename: Ian
authors.initials: I
authors.lastname: Logan
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China. zhengyt@mail.kiz.ac.cn.|Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, China. zhengyt@mail.kiz.ac.cn.
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China. yaoyg@mail.kiz.ac.cn.|Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Yao
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000502:physiology*; D006239:Haplotypes / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008297:Male; D025341:Principal Component Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D012307:Risk Factors; D015819:Substance Abuse, Intravenous / Q000235:genetics; D019966:Substance-Related Disorders / Q000235:genetics*; D019562:Viral Load / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species
keywords: Chinese; Drug addiction; HIV-1 infection; mtDNA haplogroup