title: Monoacylglycerol lipase promotes Fcγ receptor-mediated phagocytosis in microglia but does not regulate LPS-induced upregulation of inflammatory cytokines.
abstract: Monoacylglycerol lipase (MAGL) is important for neuroinflammation. However, the regulatory mechanisms underlying its expression and function remain unknown. Lipopolysaccharide (LPS) treatment post-translationally upregulated MAGL expression, whereas it downregulated MAGL transcription through a Stat6-mediated mechanism in microglia. Neither MAGL knockdown nor JZL-184, a selective MAGL inhibitor, suppressed LPS-induced upregulation of inflammatory cytokines in microglia. Moreover, exogenous expression of MAGL in BV-2 microglial cell line, which lacks endogenous MAGL, did not promote the induction of inflammatory cytokines by LPS treatment. Interestingly, MAGL knockdown reduced Fcγ receptor-mediated phagocytosis in primary microglia, and introduction of MAGL into the BV-2 cells increased Fcγ receptor-mediated phagocytosis. Collectively, these results suggest that MAGL regulates phagocytosis, but not LPS-mediated cytokine induction in microglia.
authors.affiliation: Department of Pathology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-cho, Kasugai, Aichi 480-0392, Japan. Electronic address: zkouchi@inst-hsc.jp.
authors.forename: Zen
authors.initials: Z
authors.lastname: Kouchi
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008994:Monoacylglycerol Lipases / Q000235:genetics / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D017452:Receptors, IgG / Q000502:physiology*; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D017452:Receptors, IgG; D008994:Monoacylglycerol Lipases
keywords: Cytokine; Lipopolysaccharide; Microglia; Monoacylglycerol lipase (MAGL); Phagocytosis; Stat6