title: Molecular epidemiology and phylogenetic analysis of Dengue virus type-1 and 2 isolated in Malaysia.
abstract: OBJECTIVE
  Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus.
  
  
  METHODS
  Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree.
  
  
  RESULTS
  A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype.
  
  
  CONCLUSIONS
  The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.
authors.affiliation: Muhammad Hasyim Chew, Ph.D scholar, Laboratory Public Health Kota Kinabalu, Sabah, Ministry of Health, Malaysia.
authors.forename: Muhd Hasyim
authors.initials: MH
authors.lastname: Chew
authors.affiliation: Dr. Md. Mostafizur Rahman, Professor, Department of Medical Microbiology and Immunology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
authors.forename: Md Mostafizur
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Dr. Salasawati Hussin, Associate Professor, Department of Medical Microbiology and Immunology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
authors.forename: Salasawati
authors.initials: S
authors.lastname: Hussin
keywords: DENV; E gene; Genotype; Molecular epidemiology; Phylogenetic analysis