title: The structure of the Cyberlindnera jadinii genome and its relation to Candida utilis analyzed by the occurrence of single nucleotide polymorphisms.
abstract: The yeast Cyberlindnera jadinii is a close relative of Candida utilis that is being used in the food and feed industries. Here, we present the 12.7Mb genome sequence of C. jadinii strain CBS 1600 generated by next generation sequencing. The deduced draft genome sequence consists of seven large scaffolds analogous to the seven largest chromosomes of C. utilis. An automated annotation of the C. jadinii genome identified 6147 protein-coding sequences. The level of ploidy for both genomes was analyzed by calling single nucleotide polymorphisms (SNPs) and was verified measuring nuclear DNA contents by florescence activated cell sorting (FACS). Both analyses determined the level of ploidy to diploid for C. jadinii and to triploid for C. utilis. However, SNP calling for C. jadinii also identified scaffold regions that seem to be haploid, triploid or tetraploid.
authors.affiliation: Institut für Bioinformatik und Systembiologie, Justus-Liebig-University Gießen, Gießen, Germany; Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Bielefeld, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Rupp
authors.affiliation: Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Bielefeld, Germany. Electronic address: kbrinkro@cebitec.uni-bielefeld.de.
authors.forename: Karina
authors.initials: K
authors.lastname: Brinkrolf
authors.affiliation: Institut für Molekulare Mykologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Buerth
authors.affiliation: Institut für Molekulare Mykologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
authors.forename: Maya
authors.initials: M
authors.lastname: Kunigo
authors.affiliation: Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Bielefeld, Germany.
authors.forename: Jessica
authors.initials: J
authors.lastname: Schneider
authors.affiliation: Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Bielefeld, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Jaenicke
authors.affiliation: Institut für Bioinformatik und Systembiologie, Justus-Liebig-University Gießen, Gießen, Germany; Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Bielefeld, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.affiliation: Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Bielefeld, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.affiliation: Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany; Institut für Bio- und Geowissenschaften IBG-1: Biotechnologie, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
authors.affiliation: Institut für Molekulare Mykologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
authors.forename: Joachim F
authors.initials: JF
authors.lastname: Ernst
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D001482:Base Composition / Q000235:genetics; D002175:Candida / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004171:Diploidy; D005434:Flow Cytometry; D063990:Gene Ontology; D005800:Genes, Fungal; D016681:Genome, Fungal; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal
keywords: Candida utilis; Cyberlindnera jadinii; Genome sequence; Level of ploidy; SNP (single nucleotide polymorphism) calling