title: Dimensionality of Rolled-up Nanomembranes Controls Neural Stem Cell Migration Mechanism.
abstract: We employ glass microtube structures fabricated by rolled-up nanotechnology to infer the influence of scaffold dimensionality and cell confinement on neural stem cell (NSC) migration. Thereby, we observe a pronounced morphology change that marks a reversible mesenchymal to amoeboid migration mode transition. Space restrictions preset by the diameter of nanomembrane topography modify the cell shape toward characteristics found in living tissue. We demonstrate the importance of substrate dimensionality for the migration mode of NSCs and thereby define rolled-up nanomembranes as the ultimate tool for single-cell migration studies.
authors.affiliation: †Institute for Integrative Nanosciences, Leibniz Institute for Solid State and Materials Research Dresden, D-01069 Dresden, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Koch
authors.affiliation: †Institute for Integrative Nanosciences, Leibniz Institute for Solid State and Materials Research Dresden, D-01069 Dresden, Germany.|‡Division of Neurodegenerative Diseases, Department of Neurology, Technische Universität Dresden, D-01307 Dresden, Germany.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Meyer
authors.affiliation: †Institute for Integrative Nanosciences, Leibniz Institute for Solid State and Materials Research Dresden, D-01069 Dresden, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Helbig
authors.affiliation: †Institute for Integrative Nanosciences, Leibniz Institute for Solid State and Materials Research Dresden, D-01069 Dresden, Germany.
authors.forename: Stefan M
authors.initials: SM
authors.lastname: Harazim
authors.affiliation: ‡Division of Neurodegenerative Diseases, Department of Neurology, Technische Universität Dresden, D-01307 Dresden, Germany.|§German Center for Neurodegenerative Diseases (DZNE) Dresden, D-01307 Dresden, Germany.|⊥Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, D-01307 Dresden, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Storch
authors.affiliation: †Institute for Integrative Nanosciences, Leibniz Institute for Solid State and Materials Research Dresden, D-01069 Dresden, Germany.|¶Max Planck Institute for Intelligent Systems, D-70569 Stuttgart, Germany.
authors.forename: Samuel
authors.initials: S
authors.lastname: Sanchez
authors.affiliation: †Institute for Integrative Nanosciences, Leibniz Institute for Solid State and Materials Research Dresden, D-01069 Dresden, Germany.|∥Material Systems for Nanoelectronics, Technische Universität Chemnitz, D-09107 Chemnitz, Germany.|#Center for Advancing Electronics Dresden, Technische Universität Dresden, D-01187 Dresden, Germany.
authors.forename: Oliver G
authors.initials: OG
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D005898:Glass / Q000737:chemistry; D008567:Membranes, Artificial; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D058953:Neural Stem Cells / Q000166:cytology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial
keywords: 3D cell culture scaffold; Nanomembranes; cell migration; neural stem cells; rolled-up nanotechnology; spatial confinement