title: Non-Invasive Quantification of Cartilage Using a Novel In Vivo Bioluminescent Reporter Mouse.
abstract: Mouse models are common tools for examining post-traumatic osteoarthritis (OA), which involves cartilage deterioration following injury or stress. One challenge to current mouse models is longitudinal monitoring of the cartilage deterioration in vivo in the same mouse during an experiment. The objective of this study was to assess the feasibility for using a novel transgenic mouse for non-invasive quantification of cartilage. Chondrocytes are defined by expression of the matrix protein aggrecan, and we developed a novel mouse containing a reporter luciferase cassette under the inducible control of the endogenous aggrecan promoter. We generated these mice by crossing a Cre-dependent luciferase reporter allele with an aggrecan creERT2 knockin allele. The advantage of this design is that the targeted knockin retains the intact endogenous aggrecan locus and expresses the tamoxifen-inducible CreERT2 protein from a second IRES-driven open reading frame. These mice display bioluminescence in the joints, tail, and trachea, consistent with patterns of aggrecan expression. To evaluate this mouse as a technology for non-invasive quantification of cartilage loss, we characterized the relationship between loss of bioluminescence and loss of cartilage after induction with (i) ex vivo collagenase digestion, (ii) an in vivo OA model utilizing treadmill running, and (iii) age. Ex vivo experiments revealed that collagenase digestion of the femur reduced both luciferase signal intensity and pixel area, demonstrating a link between cartilage degradation and bioluminescence. In an in vivo model of experimental OA, we found decreased bioluminescent signal and pixel area, which correlated with pathological disease. We detected a decrease in both bioluminescent signal intensity and area with natural aging from 2 to 13 months of age. These results indicate that the bioluminescent signal from this mouse may be used as a non-invasive quantitative measure of cartilage. Future studies may use this reporter mouse to advance basic and preclinical studies of murine experimental OA with applications in synovial joint biology, disease pathogenesis, and drug delivery.
authors.affiliation: Molecular Biosciences Program, Montana State University, Bozeman, MT, United States of America; Department of Mechanical and Industrial Engineering, Montana State University, Bozeman, MT, United States of America.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Mailhiot
authors.affiliation: Department of Mechanical and Industrial Engineering, Montana State University, Bozeman, MT, United States of America.
authors.forename: Donald L
authors.initials: DL
authors.lastname: Zignego
authors.affiliation: Department of Microbiology and Immunology, Montana State University, Bozeman, MT, United States of America.
authors.forename: Justin R
authors.initials: JR
authors.lastname: Prigge
authors.affiliation: Department of Mechanical and Industrial Engineering, Montana State University, Bozeman, MT, United States of America.
authors.forename: Ella R
authors.initials: ER
authors.lastname: Wardwell
authors.affiliation: Molecular Biosciences Program, Montana State University, Bozeman, MT, United States of America; Department of Microbiology and Immunology, Montana State University, Bozeman, MT, United States of America.
authors.forename: Edward E
authors.initials: EE
authors.lastname: Schmidt
authors.affiliation: Molecular Biosciences Program, Montana State University, Bozeman, MT, United States of America; Department of Mechanical and Industrial Engineering, Montana State University, Bozeman, MT, United States of America; Department of Cell Biology and Neuroscience, Affiliate Faculty, Montana State University, Bozeman, MT, United States of America.
authors.forename: Ronald K
authors.initials: RK
authors.lastname: June
mesh_terms: D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D005240:Feasibility Studies; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010003:Osteoarthritis / Q000235:genetics / Q000378:metabolism*; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D053674:Aggrecans; D050071:Bone Density Conservation Agents; D013629:Tamoxifen; D008156:Luciferases