title: Biological activity of alginate and its effect on pancreatic lipase inhibition as a potential treatment for obesity.
abstract: Alginates are classed as a dietary fibre and have been shown to inhibit digestive enzymesin vitro, and therefore could be used as an obesity treatment. The current study aims to assess whether alginate in a bread vehicle maintains its inhibition properties despite cooking and digestion, and may therefore be used as a potential treatment for obesity. After 180 min in a model gut that replicates digestion in the mouth, stomach and small intestines alginate bread (AB), control bread (CB), CB with Manucol®DM alginate, free DM alginate and model gut solution were collected. DM, LFR 5/60 and SF200 were heated at 37 °C and 200 °C, with DM also heated at 50, 100 and 150 °C. Samples from the model gut and heated alginate were assessed for molecular size and inhibition properties using viscosity, gel filtration and a lipase turbidity assay. AB does not significantly increase viscosity in the model gut. Viscosity of alginate reduces beyond 100 °C, although alginate retains its inhibition properties up to 150 °C. Cooking into the bread does not reduce the molecular size of the alginate or affect its inhibition properties. These data demonstrate the robustness of alginates lipase inhibition despite the cooking process and digestion. Therefore adding alginate to a bread vehicle may have the potential in the treatment for obesity.
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Houghton
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Wilcox
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: Peter I
authors.initials: PI
authors.lastname: Chater
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: Iain A
authors.initials: IA
authors.lastname: Brownlee
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom ; Human Nutrition Research Centre, School of Agriculture, Food & Rural Development, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Seal
authors.affiliation: Institute for Cell and Molecular Bioscience, Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Pearson
keywords: AB, alginate bread; Alginate; Biological; CB, control bread; DB, dextran blue; GI, gastrointestinal tract; Inhibition; Lipase; MO, methyl orange; Pancreatic; WHO, World Health Organisation