title: Folate-modified, cisplatin-loaded lipid carriers for cervical cancer chemotherapy.
abstract: BACKGROUND
  Cervical cancer chemotherapy calls for the efficiently delivery of anticancer drug into cancer cells by nanoparticles. In this study, folate (FA) modified, cisplatin (CIS)-loaded nanostructured lipid carriers (NLCs) were constructed and evaluated.
  
  
  METHODS
  FA containing polyethylene glycol (PEG)-distearoylphosphatidylethanolamine (DSPE) (FA-PEG-DSPE) was synthesized. FA-PEG-DSPE modified, CIS-loaded NLCs (FA-CIS-NLCs) were prepared. Their particle size, zeta potential, drug encapsulation efficiency (EE) and in vitro delivery behavior were evaluated. In vitro cytotoxicity study of FA-CIS-NLCs was tested in human cervix adenocarcinoma cell line (HeLa cells). In vivo anti-tumor efficacies of the carriers were evaluated on a mice-bearing cervical cancer model.
  
  
  RESULTS
  The optimum FA-CIS-NLCs formulations have a particle size of 143.2 nm and a +25.7 mV surface charge. FA-CIS-NLCs displayed the best anti-tumor activity than other formulations in vitro and in vivo.
  
  
  CONCLUSIONS
  The results demonstrated that FA-CIS-NLCs were efficient in selective delivery to cancer cells over-expressing FA receptors (FRs). FA-CIS-NLCs targeted transfer CIS to the cervical cancer cells, enhance the anti-tumor capacity. The novel constructed NLCs could function as outstanding nanocarriers for the delivery of drugs for the targeted treatment of cervical cancers.
authors.affiliation: a Department of Gynecology and Obstetrics , The Fourth People's Hospital of Ji'nan , Ji'nan, Shandong , People's Republic of China and.
authors.forename: Guilian
authors.initials: G
authors.lastname: Zhang
authors.affiliation: a Department of Gynecology and Obstetrics , The Fourth People's Hospital of Ji'nan , Ji'nan, Shandong , People's Republic of China and.
authors.forename: Fengying
authors.initials: F
authors.lastname: Liu
authors.affiliation: a Department of Gynecology and Obstetrics , The Fourth People's Hospital of Ji'nan , Ji'nan, Shandong , People's Republic of China and.
authors.forename: Erxia
authors.initials: E
authors.lastname: Jia
authors.affiliation: b Department of Gynecology and Obstetrics , Qilu Hospital of Shandong University , Ji'nan, Shandong , People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jia
authors.affiliation: b Department of Gynecology and Obstetrics , Qilu Hospital of Shandong University , Ji'nan, Shandong , People's Republic of China.
authors.forename: Youzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; D010714:Phosphatidylethanolamines; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D005492:Folic Acid; D002945:Cisplatin
keywords: Cervical cancer; HeLa cells; chemotherapy; folate modified; nanostructured lipid carriers