title: Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana.
abstract: Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.
authors.affiliation: †Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yin-Shiou
authors.initials: YS
authors.lastname: Lin
authors.affiliation: §Department of Life Science, National Taitung University, Taitung, Taiwan.
authors.forename: Chiy-Rong
authors.initials: CR
authors.lastname: Chen
authors.affiliation: #Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors.forename: Wei-Hau
authors.initials: WH
authors.lastname: Wu
authors.affiliation: ⊥Taiwan Seed Improvement and Propagation Station, Council of Agriculture, Taichung, Taiwan.|‡Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
authors.forename: Chi-Luan
authors.initials: CL
authors.lastname: Wen
authors.affiliation: #Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors.forename: Chi-I
authors.initials: CI
authors.lastname: Chang
authors.affiliation: †Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.|‡Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Hou
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000494:pharmacology*; D018149:Glucose Intolerance / Q000188:drug therapy; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009765:Obesity / Q000150:complications; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013552:Swine; D027843:Vitis; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D001572:Benzofurans; D054873:Dipeptidyl-Peptidase IV Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010636:Phenols; D010936:Plant Extracts; D013267:Stilbenes; C518233:hopeaphenol; C480486:malvidin-3-O-glucoside-4 vinyl; C412469:vitisin A; D000520:alpha-Glucosidases; D018819:Dipeptidyl Peptidase 4
keywords: (+)-vitisin A; (−)-vitisin B; Vitis thunbergii var. taiwaniana (VTT); blood sugar; dipeptidyl peptidase-IV (DPP-IV); impaired glucose tolerance; α-glucosidase