title: Isolation of Foreign Material-Free Endothelial Progenitor Cells Using CD31 Aptamer and Therapeutic Application for Ischemic Injury.
abstract: Endothelial progenitor cells (EPCs) can be isolated from human bone marrow or peripheral blood and reportedly contribute to neovascularization. Aptamers are 40-120-mer nucleotides that bind to a specific target molecule, as antibodies do. To utilize apatmers for isolation of EPCs, in the present study, we successfully generated aptamers that recognize human CD31, an endothelial cell marker. CD31 aptamers bound to human umbilical cord blood-derived EPCs and showed specific interaction with human CD31, but not with mouse CD31. However, CD31 aptamers showed non-specific interaction with CD31-negative 293FT cells and addition of polyanionic competitor dextran sulfate eliminated non-specific interaction without affecting cell viability. From the mixture of EPCs and 293FT cells, CD31 aptamers successfully isolated EPCs with 97.6% purity and 94.2% yield, comparable to those from antibody isolation. In addition, isolated EPCs were decoupled from CD31 aptamers with a brief treatment of high concentration dextran sulfate. EPCs isolated with CD31 aptamers and subsequently decoupled from CD31 aptamers were functional and enhanced the restoration of blood flow when transplanted into a murine hindlimb ischemia model. In this study, we demonstrated isolation of foreign material-free EPCs, which can be utilized as a universal protocol in preparation of cells for therapeutic transplantation.
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Yoon
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Il Ho
authors.initials: IH
authors.lastname: Jang
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Soon Chul
authors.initials: SC
authors.lastname: Heo
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Yang Woo
authors.initials: YW
authors.lastname: Kwon
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.affiliation: Departments of Obstetrics and Gynecology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Suh
authors.affiliation: Departments of Obstetrics and Gynecology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Seungmin
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.affiliation: Aptamer Sciences Inc. Postech Biotech Center, San 31 Hyoja-Dong, Pohang 790-784, South Korea.
authors.forename: Jongha
authors.initials: J
authors.lastname: Jung
authors.affiliation: Aptamer Sciences Inc. Postech Biotech Center, San 31 Hyoja-Dong, Pohang 790-784, South Korea.
authors.forename: Kiseok
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea; Research Institute of Convergence Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 626-770, Gyeongsangnam-do, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000378:metabolism / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism; D057809:HEK293 Cells; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007511:Ischemia / Q000378:metabolism / Q000628:therapy*; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D033581:Stem Cell Transplantation / Q000379:methods
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D019408:Platelet Endothelial Cell Adhesion Molecule-1