title: Yin Yang 1 is a multi-functional regulator of adipocyte differentiation in 3T3-L1 cells.
abstract: Yin Yang 1 (YY1) is an ubiquitously distributed transcription factor that belongs to the GLI-Kruppel class of zinc finger proteins. The mechanism by which YY1 regulates adipocyte differentiation remains unclear. In this study, we investigated the functional role of YY1 during adipocyte differentiation. During the early stage, YY1 gene and protein expression was transiently downregulated upon the induction of differentiation, however, it was consistently induced during the later stage. YY1 overexpression decreased adipocyte differentiation and blocked cell differentiation at the preadipocyte stage, while YY1 knockdown by RNA interference increased adipocyte differentiation. YY1 physically interacted with PPARγ (Peroxisome proliferator-activated receptor gamma) and C/EBPβ (CCAAT/enhancer-binding protein beta) respectively in 3T3-L1 cells. Through its interaction with PPARγ, YY1 directly decreased PPARγ transcriptional activity. YY1 ectopic expression prevented C/EBPβ from binding to the PPARγ promoter, resulting in the downregulation of PPARγ transcriptional activity. These results indicate that YY1 repressed adipocyte differentiation by repressing the activity of adipogenic transcriptional factors in 3T3-L1 cells.
authors.affiliation: College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea.
authors.forename: Younho
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea.
authors.forename: You Hee
authors.initials: YH
authors.lastname: Choi
authors.affiliation: College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea.
authors.forename: Yun-Hye
authors.initials: YH
authors.lastname: Jin
authors.affiliation: Research Institute, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 410-769, South Korea. Electronic address: heesunch@ncc.re.kr.
authors.forename: Heesun
authors.initials: H
authors.lastname: Cheong
authors.affiliation: College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea. Electronic address: kwanglee@chonnam.ac.kr.
authors.forename: Kwang Youl
authors.initials: KY
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D050986:YY1 Transcription Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D047495:PPAR gamma; D050986:YY1 Transcription Factor; C494699:Yy1 protein, mouse
keywords: 3T3-L1; Adipocyte differentiation; C/EBPα; C/EBPβ; PPARγ; YY1