title: Scaffold electrodes based on thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and Azure A films for high performance biosensor.
abstract: Nanometric size gold nanoparticles capped with thiotic acid are used to coordinate with the Zn (II) present in the catalytic center of Alcohol Dehydrogenase (ADH). In combination with the NADH oxidation molecular catalyst Azure A, electrografted onto carbon screen-printed electrodes, they are used as scaffold electrodes for the construction of a very efficient ethanol biosensor. The final biosensing device exhibits a highly efficient ethanol oxidation with low overpotential of -0.25 V besides a very good analytical performance with a detection limit of 0.14±0.01 μM and a stable response for more than one month.
authors.affiliation: Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: cesar.anquela@uam.es.
authors.forename: C
authors.initials: C
authors.lastname: Gómez-Anquela
authors.affiliation: Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: tania.garcia@uam.es.
authors.forename: T
authors.initials: T
authors.lastname: García-Mendiola
authors.affiliation: Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: josemaria.abad@uam.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Abad
authors.affiliation: Instituto de Catalisis y Petroleoquimica, CSIC. C/ Marie Curie, 2, L10., 28049 Madrid, Spain. Electronic address: marcospita@icp.csic.es.
authors.forename: M
authors.initials: M
authors.lastname: Pita
authors.affiliation: Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain. Electronic address: felix.pariente@uam.es.
authors.forename: F
authors.initials: F
authors.lastname: Pariente
authors.affiliation: Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049, Spain; IMDEA Nanociencia, Madrid, Spain. Electronic address: encarnacion.lorenzo@uam.es.
authors.forename: E
authors.initials: E
authors.lastname: Lorenzo
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry; D001399:Azure Stains / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002384:Catalysis; D004566:Electrodes; D004867:Equipment Design; D000431:Ethanol / Q000032:analysis*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D006046:Gold; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D008063:Thioctic Acid / Q000737:chemistry; D014920:Wine / Q000032:analysis
chemical_list: D001399:Azure Stains; D000431:Ethanol; D008063:Thioctic Acid; D006046:Gold; D000426:Alcohol Dehydrogenase; C025818:Azure A
keywords: Alcohol dehydrogenase; Biosensor; Electrografting; Gold nanoparticles