title: Structural and ultrastructural evaluation of the aortic wall after transplantation of bone marrow-derived cells (BMCs) in a model for atherosclerosis.
abstract: Stem cells are characterized by their ability to differentiate into multiple cell lineages and display the paracrine effect. The aim of this work was to evaluate the effect of therapy with bone marrow-derived cells (BMCs) on glucose, lipid metabolism, and aortic wall remodeling in mice through the administration of a high-fat diet and subsequent BMCs transplantation. C57BL/6 mice were fed a control diet (CO group) or an atherogenic diet (AT group). After 16 weeks, the AT group was divided into 4 subgroups: an AT 14 days group and AT 21 days group that were given an injection of vehicle and sacrificed after 14 and 21 days, respectively, and an AT-BMC 14 days group and AT-BMC 21 days group that were given an injection of BMCs and sacrificed after 14 and 21 days, respectively. The BMCs transplant had reduced blood glucose, triglycerides, and total cholesterol. There was no significant difference in relation to body mass between the transplanted groups and non-transplanted groups, and all were different than CO. There was no significant difference in the glycemic curve among AT 14 days, AT-BMC 14 days, and AT 21 days, and these were different than the CO and the AT-BMC 21 days groups. The increased thickness of the aortic wall was observed in all atherogenic groups, but was significantly smaller in group AT-BMC 21 days compared to AT 14 days and AT 21 days. Vacuoles in the media tunic, delamination and the thinning of the elastic lamellae were observed in AT 14 days and AT 21 days. The smallest number of these was displayed on the AT-BMC 14 days and AT-BMC 21 days. Marking to CD105, CD133, and CD68 were observed in AT 14 days and AT 21 days. These markings were not observed in AT-BMC 14 days or in AT-BMC 21 days. Electron micrographs show the beneficial remodeling in AT-BMC 14 days and AT-BMC 21 days, and the structural organization was similar to the CO group. Vesicles of pinocytosis, projection of smooth muscle cells, and delamination of the internal elastic lamina are seen in groups AT 14 days and AT 21 days. Endothelial cells were preserved, and regular and continuous contour in internal elastic lamelae were observed in the CO, the AT-BMC 14 days, and AT-BMC 21 days groups. In conclusion, in an atherosclerotic model using mice and atherogenic diet, the injection of BMCs improves glucose, lipid metabolism, and causes a beneficial remodeling of the aortic wall.
authors.affiliation: a Laboratory of Ultrastructure and Tissue Biology, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, (UERJ), Bouvelard 28 de Setembro Avn, 77, Vila Isabel, Cep 20.551-030 Rio de Janeiro, Brazil.
authors.forename: Alyne Souza
authors.initials: AS
authors.lastname: Felix
authors.affiliation: a Laboratory of Ultrastructure and Tissue Biology, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, (UERJ), Bouvelard 28 de Setembro Avn, 77, Vila Isabel, Cep 20.551-030 Rio de Janeiro, Brazil.
authors.forename: Nemesis
authors.initials: N
authors.lastname: Monteiro
authors.affiliation: c Department of Veterinary medicine, Federal University of Juiz de Fora, Minas Gerais, Brazil.
authors.forename: Vinícius Novaes
authors.initials: VN
authors.lastname: Rocha
authors.affiliation: b Research Laboratory of Stem Cells, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Genilza
authors.initials: G
authors.lastname: Oliveira
authors.affiliation: a Laboratory of Ultrastructure and Tissue Biology, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, (UERJ), Bouvelard 28 de Setembro Avn, 77, Vila Isabel, Cep 20.551-030 Rio de Janeiro, Brazil.
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Nascimento
authors.affiliation: b Research Laboratory of Stem Cells, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Laís
authors.initials: L
authors.lastname: de Carvalho
authors.affiliation: b Research Laboratory of Stem Cells, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Thole
authors.affiliation: a Laboratory of Ultrastructure and Tissue Biology, Histology and Embryology Department, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, (UERJ), Bouvelard 28 de Setembro Avn, 77, Vila Isabel, Cep 20.551-030 Rio de Janeiro, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology* / Q000648:ultrastructure; D050197:Atherosclerosis / Q000628:therapy*; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D016026:Bone Marrow Transplantation; D002784:Cholesterol / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D014280:Triglycerides; D002784:Cholesterol
keywords: C57BL/6; aorta remodeling; bone marrow cells; cellules de moelle osseuse; diète riche en gras; high fat diet; remodelage aortique