title: Preparation and characterization of alginate-gelatin microencapsulated Bacillus subtilis SL-13 by emulsification/internal gelation.
abstract: Gelatin was blended with sodium alginate (NaALG) to obtain a novel microbial fungicide, and dispersed micron Bacillus subtilis SL-13 microspheres prepared by emulsification/internal gelation method. Microscopic examination revealed that microcapsules were nearly spherical in shape. Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction confirmed that the electrostatic interaction was occurred when gelatin added into NaALG. The maximum encapsulation efficiency was 93.44% at a gelatin concentration of 1.5%. Particle size, swelling, and biodegradation of beads increased with gelatin content increase. Furthermore, the viability of encapsulated SL-13 could be preserved at more than 10(8) CFU/mL after 120 d storage at 25 °C. The number of viable cells released from microcapsules presented an initial rapid increase followed by a gradual increase, and reached the maximum as 10(10) CFU/mL on day 35. Thus, it is feasible to prepare uniform, rounded shape, and well-dispersed micron microcapsules of SL-13 via emulsification/internal gelation using NaALG and gelatin composites. This encapsulation strategy could be considered as a potential alternative to future applications in the agricultural industry.
authors.affiliation: a School of Chemistry and Chemical Engineering , Shihezi University , Shihezi 832003 , P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tu
authors.forename: Yanhui
authors.initials: Y
authors.lastname: He
authors.forename: Hongbing
authors.initials: H
authors.lastname: Yang
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Wu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yi
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D004339:Drug Compounding; D065546:Drug Liberation; D004655:Emulsions; D005780:Gelatin / Q000737:chemistry*; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D050296:Microbial Viability; D008863:Microspheres; D010316:Particle Size; D014867:Water / Q000032:analysis
chemical_list: D000464:Alginates; D004337:Drug Carriers; D004655:Emulsions; D005782:Gels; D006603:Hexuronic Acids; D014867:Water; D020723:Glucuronic Acid; D005780:Gelatin
keywords: Bacillus subtilis SL-13; emulsification internal gelation; gelatin; microcapsule; sodium alginate