title: Identification of Hop stunt viroid infecting Citrus limon in China using small RNAs deep sequencing approach.
abstract: BACKGROUND
  The advent of next generation sequencing technology has allowed for significant advances in plant virus discovery, particularly for identification of covert viruses and previously undescribed viruses. The Citrus limon Burm. f. (C. limon) is a small evergreen tree native to Asia, and . China is the world's top lemon-producing nation.
  
  
  RESULTS
  In this work, lemon samples were collected from southwestern of China, where an unknown disease outbreak had caused huge losses in the lemon production industry. Using high-throughput pyrosequencing and the assembly of small RNAs, we showed that the Hop stunt viroid (HSVd) was present in C. limon leaf sample. The majority of it is a main lemon producing agricultural cultivarHop stunt viroid derived siRNAs (HSVd-siRNAs) in C. limon were 21 nucleotides in length, and nearly equal amount of HSVd-siRNAs originated from the plus-genomic RNA strand as from the complementary strand. A bias of HSVd-siRNAs toward sequences beginning with a 5'-Guanine was observed. Furthermore, hotspot analysis showed that a large amount of HSVd-siRNAs derived from the central and variant domains of the HSVd genome.
  
  
  CONCLUSIONS
  Our results suggest that C. limon could set up a small RNA-mediated gene silencing response to Hop stunt viroid, Interestingly, based on bioinformatics analysis, our results also suggest that the large amounts of HSVd-siRNAs from central and variant domains might be involved in interference with host gene expression and affect symptom development.
authors.affiliation: Institute of Biotechnology, State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, 310029, People's Republic of China. suxiu@zafu.edu.cn.
authors.forename: Xiu
authors.initials: X
authors.lastname: Su
authors.affiliation: Institute of Biotechnology, State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, 310029, People's Republic of China. 450682659@qq.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Fu
authors.affiliation: Institute of Biotechnology, State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, 310029, People's Republic of China. yjqian@zju.edu.cn.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Institute of Biotechnology, State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, 310029, People's Republic of China. xuyiqdpd@zju.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Biotechnology, State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, 310029, People's Republic of China. zzhou@zju.edu.cn.|Institute of Plant Protection, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China. zzhou@zju.edu.cn.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002681:China; D002957:Citrus / Q000821:virology*; D020868:Gene Silencing; D059014:High-Throughput Nucleotide Sequencing; D010935:Plant Diseases / Q000821:virology*; D058727:RNA, Small Untranslated / Q000737:chemistry* / Q000235:genetics*; D017385:Sequence Homology; D014772:Viroids / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D058727:RNA, Small Untranslated