title: Evolved pesticide tolerance in amphibians: Predicting mechanisms based on pesticide novelty and mode of action.
abstract: We examined 10 wood frog populations distributed along an agricultural gradient for their tolerance to six pesticides (carbaryl, malathion, cypermethrin, permethrin, imidacloprid, and thiamethoxam) that differed in date of first registration (pesticide novelty) and mode-of-action (MOA). Our goals were to assess whether: 1) tolerance was correlated with distance to agriculture for each pesticide, 2) pesticide novelty predicted the likelihood of evolved tolerance, and 3) populations display cross-tolerance between pesticides that share and differ in MOA. Wood frog populations located close to agriculture were more tolerant to carbaryl and malathion than populations far from agriculture. Moreover, the strength of the relationship between distance to agriculture and tolerance was stronger for older pesticides compared to newer pesticides. Finally, we found evidence for cross-tolerance between carbaryl and malathion (two pesticides that share MOA). This study provides one of the most comprehensive approaches for understanding patterns of evolved tolerance in non-pest species.
authors.affiliation: Biological Sciences Department, Binghamton University (SUNY), Binghamton, NY 13902, USA. Electronic address: jhua@binghamton.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Hua
authors.affiliation: Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Devin K
authors.initials: DK
authors.lastname: Jones
authors.affiliation: Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Mattes
authors.affiliation: Department of Biological Sciences, Southwestern Oklahoma State University, Weatherford, OK 73096, USA.
authors.forename: Rickey D
authors.initials: RD
authors.lastname: Cothran
authors.affiliation: Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Rick A
authors.initials: RA
authors.lastname: Relyea
authors.affiliation: Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Hoverman
mesh_terms: D000818:Animals; D012721:Carbaryl / Q000494:pharmacology; D004361:Drug Tolerance; D005260:Female; D008294:Malathion / Q000494:pharmacology; D008297:Male; D010575:Pesticides / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology; D011898:Ranidae / Q000502:physiology*
chemical_list: D010575:Pesticides; D011722:Pyrethrins; C017160:cypermethrin; D012721:Carbaryl; D008294:Malathion
keywords: Carbamates; Lithobates sylvaticus; Neonicotinoid; Organophosphates; Pyrethroids