title: Validation of a sensitive HPLC/fluorescence method for assessment of ciprofloxacin levels in plasma and prostate microdialysate samples from rats.
abstract: Chronic bacterial prostatitis treatment consists of broad-spectrum antibiotic therapy for long periods of time. Drug penetration into the prostate makes the treatment a challenged. Ciprofloxacin is one of the most prescribed drugs for this treatment. A liquid chromatography with fluorescence detection method was developed and validated for determining ciprofloxacin concentrations in two different matrices: plasma and prostate microdialysate. Ciprofloxacin was separated on a C18 column eluted with a mobile phase constituted of a mixture of 0.4% aqueous triethylamine:methanol:acetonitrile (75:15:10, v/v/v) and 0.4% aqueous triethylamine:acetonitrile (88:12, v/v) for microdialysate and plasma samples, respectively. Linearity was obtained over a concentration range of 5-1000 ng/mL (microdialysate) and 10-2000 ng/mL (plasma), with coefficients of determination ≥0.9956. Precision was determined from the analysis of six quality control samples and showed RSD values <11.1 and 7.4% for intra and inter-assay precision, respectively. The accuracy ranged from 85.6 to 114.3%. The method was applied to a preliminary pharmacokinetic study to investigate ciprofloxacin concentrations in prostate, sampled by microdialysis, and plasma after a 7 mg/kg intravenous dose to Wistar rats. The method showed high sensitivity using only protein precipitation as plasma sample clean-up and was successfully applied to investigate ciprofloxacin prostate penetration.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas/UFRGS, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90.610-000, Brazil.|Centro Bioanalítico de Medicamentos, Faculdade de Farmácia, UFRGS, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90.610-000, Brazil.
authors.forename: Estevan Sonego
authors.initials: ES
authors.lastname: Zimmermann
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas/UFRGS, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90.610-000, Brazil.|Centro Bioanalítico de Medicamentos, Faculdade de Farmácia, UFRGS, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90.610-000, Brazil.
authors.forename: Bruna Gaelzer Silva
authors.initials: BG
authors.lastname: Torres
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas/UFRGS, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90.610-000, Brazil.|Centro Bioanalítico de Medicamentos, Faculdade de Farmácia, UFRGS, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90.610-000, Brazil.
authors.forename: Teresa
authors.initials: T
authors.lastname: Dalla Costa
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000097:blood* / Q000493:pharmacokinetics; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D017551:Microdialysis; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D002939:Ciprofloxacin
keywords: ciprofloxacin; liquid chromatography/fluorescence; microdialysis; pharmacokinetics; validation