title: Effects of high-fat diet on 1,2-dimethylhydrazine-induced aberrant crypt foci and colorectal tumours in rats.
abstract: Obesity is one of the leading causes of numerous types of cancer. The present study investigated the impact of a high-fat diet on 1,2-dimethylhydrazine (DMH)-induced colorectal cancer (CRC) in F344 rats. A total of 16 male F344 rats aged 4 weeks were randomly divided into two groups (8 rats/group). Rats in group A were fed a basal diet with a moderate fat (MF) content, while rats in group B were fed a high-fat diet. Upon reaching 5 weeks of age, the rats were injected subcutaneously with DMH (20 mg/kg body weight). DMH was administered once a week for 8 consecutive weeks. All the rats were sacrificed 34 weeks after the first DMH injection and dissected to obtain samples of colorectal tissues. The tissues were examined under a microscope for the presence of aberrant crypt foci (ACFs) and subjected to histopathological analysis. The results showed that at the end of the 34-week experiment, body weights and visceral fat levels were significantly higher in the high-fat diet group compared to the basal diet group. In addition, the incidences of colorectal ACF, adenoma and adenocarcinoma were markedly elevated in the high-fat diet group compared to the basal diet group. These results indicate that the consumption of a high-fat diet promotes the development and progression of CRC and the control of fat intake may prevent CRC.
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China ; Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Guangying
authors.initials: G
authors.lastname: Qi
authors.affiliation: Department of Hepatobiliary and Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors.forename: B O
authors.initials: BO
authors.lastname: Tang
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China ; Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Jikihara
authors.affiliation: Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Atsumi
authors.initials: A
authors.lastname: Kiwata
authors.affiliation: Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sakamoto
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Hepatobiliary and Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors.forename: Zhenran
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Sien
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimamoto
keywords: F344 rats; antitumour; colorectal cancer; high-fat diet