title: The Cryptococcus neoformans capsule: lessons from the use of optical tweezers and other biophysical tools.
abstract: The fungal pathogen Cryptococcus neoformans causes life-threatening infections in immunocompromised individuals, representing one of the leading causes of morbidity and mortality in AIDS patients. The main virulence factor of C. neoformans is the polysaccharide capsule; however, many fundamental aspects of capsule structure and function remain poorly understood. Recently, important capsule properties were uncovered using optical tweezers and other biophysical techniques, including dynamic and static light scattering, zeta potential and viscosity analysis. This review provides an overview of the latest findings in this emerging field, explaining the impact of these findings on our understanding of C. neoformans biology and resistance to host immune defenses.
authors.affiliation: Laboratório de Pinças Óticas da Coordenação de Programas de Estudos Avançados, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro , Rio de Janeiro, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pontes
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro , Rio de Janeiro, Brazil.
authors.forename: Susana
authors.initials: S
authors.lastname: Frases
keywords: Cryptococcus neoformans; capsule; light scattering; optical tweezers; polysaccharide; viscosity; zeta potential