title: Enhancing the Bioconversion of Winery and Olive Mill Waste Mixtures into Lignocellulolytic Enzymes and Animal Feed by Aspergillus uvarum Using a Packed-Bed Bioreactor.
abstract: Wineries and olive oil industries are dominant agro-industrial activities in southern European regions. Olive pomace, exhausted grape marc, and vine shoot trimmings are lignocellulosic residues generated by these industries, which could be valued biotechnologically. In the present work these residues were used as substrate to produce cellulases and xylanases through solid-state fermentation using Aspergillus uvarum MUM 08.01. For that, two factorial designs (3(2)) were first planned to optimize substrate composition, temperature, and initial moisture level. Subsequently, the kinectics of cellulolytic enzyme production, fungal growth, and fermented solid were characterized. Finally, the process was performed in a packed-bed bioreactor. The results showed that cellulase activity improved with the optimization processes, reaching 33.56 U/g, and with the packed-bed bioreactor aeration of 0.2 L/min, reaching 38.51 U/g. The composition of fermented solids indicated their potential use for animal feed because cellulose, hemicellulose, lignin, and phenolic compounds were partially degraded 28.08, 10.78, 13.3, and 28.32%, respectively, crude protein was increased from 8.47 to 17.08%, and the mineral contents meet the requirements of main livestock.
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho , Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Salgado
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho , Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: Abrunhosa
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho , Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Armando
authors.initials: A
authors.lastname: Venâncio
authors.affiliation: Department of Chemical Engineering, Sciences Faculty, University of Vigo (Campus Ourense) , As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Domínguez
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho , Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Belo
mesh_terms: D000821:Animal Feed / Q000032:analysis* / Q000382:microbiology; D000818:Animals; D001230:Aspergillus / Q000201:enzymology / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors / Q000382:microbiology*; D044602:Cellulases / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism; D007218:Industrial Microbiology; D008031:Lignin / Q000378:metabolism; D031658:Olea / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D027843:Vitis / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D014866:Waste Products / Q000032:analysis*
chemical_list: D056804:Biofuels; D005656:Fungal Proteins; D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D044602:Cellulases
keywords: animal feed; cellulases; olive mill wastes; packed-bed bioreactor; solid-state fermentation; winery wastes