title: Direct high-throughput amplification and sequencing of immunoglobulin genes from single human B cells.
authors.affiliation: Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Murugan
authors.affiliation: Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.|Division of B Cell Immunology, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Imkeller
authors.affiliation: Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.|Division of B Cell Immunology, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Christian E
authors.initials: CE
authors.lastname: Busse
authors.affiliation: Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.|Division of B Cell Immunology, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Hedda
authors.initials: H
authors.lastname: Wardemann
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D017931:DNA Primers / Q000138:chemical synthesis / Q000378:metabolism; D005803:Genes, Immunoglobulin; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000235:genetics* / Q000276:immunology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D059010:Single-Cell Analysis
chemical_list: D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains
keywords: B cell; Human antibody repertoire; Ig heavy chain; Ig light chain; Single cell PCR