title: AMP/GMP Analogs as Affinity ESIPT Probes for Highly Selective Sensing of Alkaline Phosphatase Activity in Living Systems.
abstract: Current probes for alkaline phosphatase (ALP) detection had been developed mainly by adding a phosphate group to a dye, which would lead to indistinct performance when implemented in a living system as several phosphatases exist together. In this study, the nucleotides adenosine monophosphate (AMP) and guanosine monophosphate (GMP) were introduced into 2'-(2'-hydroxyphenyl)-benzothiazole-based probes, and highly fluorescent turn-on probes with good selectivity towards ALP over several phosphatases, as well as high affinity and low toxicity were obtained. In the presence of L-phenylalanine, an ALP inhibitor, a strong decrease in fluorescence recovery was observed. These probes allowed for real-time imaging of endogenous ALP activity in living cells as well as in a zebrafish model.
authors.affiliation: State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian, 116023, P. R. China.|Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian, 116023, P. R. China.|Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian, 116023, P. R. China. klhan@dicp.ac.cn.|Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P. R. China. klhan@dicp.ac.cn.
authors.forename: Keli
authors.initials: K
authors.lastname: Han
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D015027:Zebrafish
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D000469:Alkaline Phosphatase; C005465:benzothiazole
keywords: AMP; GMP; alkaline phophatase; fluorescence; selectivity