title: Expression of Na+/H+ exchanger regulatory factor 1 in autosomal-dominant polycystic kidney disease.
abstract: OBJECTIVE
  To explore the role of Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) in autosomal-dominant polycystic kidney disease (ADPKD).
  
  
  METHODS
  NHERF1 and β-catenin protein were detected by immunohistochemistry and Western blotting of kidney tissue samples from patients with ADPKD and controls (normal kidney tissue [>5 cm from the foci] collected from patients undergoing unilateral nephrectomy for kidney cancer). NHERF1 and β-catenin protein and mRNA were quantified by Western blot and real-time fluorescent quantitative polymerase chain reaction, respectively, in kidney tissue samples from Han:SPRD (+/+) and (cy/+) rats. The effects of human recombinant NHERF1 on proliferation and cell cycle of ADPKD cyst-lining epithelial cells (WT9-12) were evaluated by MTT assay and flow cytometry, respectively.
  
  
  RESULTS
  Levels of NHERF1 protein and mRNA were significantly lower, and β-catenin levels significantly higher, in patients with ADPKD and Han:SPRD (cy/+) rats, compared with control subjects and (+/+) rats, respectively. Exogenous recombinant NHERF1 significantly inhibited proliferation of WT9-12 cells and increased the proportion of cells in G(0)/G(1) phase.
  
  
  CONCLUSIONS
  ADPKD is associated with a decrease in NHERF1 protein and mRNA levels. Supplementing exogenous NHERF1 inhibited the proliferation of WT9-12 cells.
authors.affiliation: Department of Nephrology, Second Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, First People's Hospital of Yuhang District, Hangzhou, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Qin
authors.affiliation: Department of Rheumatology, Second Affiliated Hospital of Zhejiang University, Hangzhou, China zhuliangdr@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers; D051176:beta Catenin; C093376:sodium-hydrogen exchanger regulatory factor
keywords: Na+/H+ exchanger regulatory factor 1; autosomal-dominant polycystic kidney disease (ADPKD; cell cycle; cell proliferation