title: A comparative study for adsorption of lysozyme from aqueous samples onto Fe3O4 magnetic nanoparticles using different ionic liquids as modifier.
abstract: In this paper, nanoparticles of Fe3O4 as well as their modified forms with different ionic liquids (IL-Fe3O4) were prepared and used for adsorption of lysozyme. The mean size and the surface morphology of the nanoparticles were characterized by TEM, XRD and FTIR techniques. Adsorption studies of lysozyme were performed under different experimental conditions in batch system on different modified magnetic nanoparticles such as, lysozyme concentration, pH of the solution, and contact time. Experimental results were obtained under the optimum operational conditions of pH 9.0 and a contact time of 10 min when initial protein concentrations of 0.05-2.0 mg mL(-1) were used. The isotherm evaluations revealed that the Langmuir model attained better fits to the equilibrium data than the Freundlich model. The maximum obtained adsorption capacities were 370.4, 400.0 500.0 and 526.3 mg of lysozyme for adsorption onto Fe3O4 and modified magnetic nanoparticles by [C4MIM][Br], [C6MIM][Br] and [C8MIM][Br] per gram of adsorbent, respectively. The Langmuir adsorption constants were 0.004, 0.019, 0.024 and 0.012 L mg(-1) for adsorptions of lysozyme onto Fe3O4 and modified magnetic nanoparticles by [C4MIM][Br], [C6MIM][Br] and [C8MIM][Br], respectively. The adsorption capacity of lysozyme was found to be dependent on its chemical structure, pH of the solution, temperature and type of ionic liquid as modifier. The applicability of two kinetic models including pseudo-first order and pseudo-second order model was estimated. Furthermore, the thermodynamic parameters were calculated. Protein could desorb from IL-Fe3O4 nanoparticles by using NaCl solution at pH 9.5 and was reused.
authors.affiliation: Professor Massoumi Laboratory, Department of Chemistry, College of Science, Shiraz University, 71454, Shiraz, Islamic Republic of Iran.|Department of Chemistry, Payame Noor University, Tehran, Islamic Republic of Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Kamran
authors.affiliation: Professor Massoumi Laboratory, Department of Chemistry, College of Science, Shiraz University, 71454, Shiraz, Islamic Republic of Iran. gubsulun@yahoo.com.
authors.forename: Ghodratollah
authors.initials: G
authors.lastname: Absalan
authors.affiliation: Professor Massoumi Laboratory, Department of Chemistry, College of Science, Shiraz University, 71454, Shiraz, Islamic Republic of Iran.
authors.forename: Mozaffar
authors.initials: M
authors.lastname: Asadi
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D004529:Egg White; D005290:Ferric Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics
chemical_list: D005290:Ferric Compounds; D052578:Ionic Liquids; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D012965:Sodium Chloride; D009113:Muramidase
keywords: Adsorption; Ionic liquid; Lysozyme; Magnetic nanoparticle