title: A New Organic Dye-Based Staining for The Detection of Plant DNA in Agarose Gels.
abstract: Ethidium bromide (EtBr) is used to stain DNA in agarose gel electrophoresis, but this dye is mutagenic and carcinogenic. We investigated N-719, which is a visible, reliable and organic Ruthenium-based dye, and five fluorescent alternatives for staining plant DNA. For prestaining and poststaining, N-719, GelRed, and SYBR Safe stained both DNA and PCR product bands as clearly as EtBr. SYBR Green I, methylene blue, and crystal violet were effective for poststaining only. The organic dye N-719 stained DNA bands as sensitively and as clearly as EtBr. Consequently, organic dyes can be used as alternatives to EtBr in plant biotechnology studies.
authors.affiliation: a Karamanoglu Mehmetbey University , Department of Bioengineering , Karaman , Turkey.
authors.forename: Özlem Ateş
authors.initials: ÖA
authors.lastname: Sönmezoğlu
authors.forename: Kerime
authors.initials: K
authors.lastname: Özkay
mesh_terms: D004396:Coloring Agents / Q000737:chemistry*; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013861:Thiocyanates / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D018744:DNA, Plant; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D013861:Thiocyanates; C506095:bis(2,2'-bipyridyl-4,4'-dicarboxylate)ruthenium(II) bis(tetrabutylammonium) bis(thiocyanate)
keywords: DNA staining; gel electrophoresis; organic dye