title: Investigation of biochemical property changes in activation-induced CD8+ T cell apoptosis using Raman spectroscopy.
abstract: The study was to investigate the changes in biochemical properties of activated mature CD8+ T cells related to apoptosis at a molecular level. We confirmed the activation and apoptosis of CD8+ T cells by fluorescence-activated cell sorting and atomic force microscopy and then performed Raman spectral measurements on activated mature CD8+ T cells and cellular deoxyribose nucleic acid (DNA). In the activated mature CD8+ T cells, there were increases in protein spectra at 1002 and 1234 cm -1 . In particular, to assess the apoptosis-related DNA spectral signatures, we investigated the spectra of the cellular DNA isolated from resting and activated mature CD8+ T cells. Raman spectra at 765 to 786  cm -1 and 1053 to 1087  cm -1 were decreased in activated mature DNA. In addition, we analyzed Raman spectrum using the multivariate statistical method including principal component analysis. Raman spectra of activated mature DNA are especially well-discriminated from those of resting DNA. Our findings regarding the biochemical and structural changes associated with apoptosis in activated mature T cells and cellular DNA according to Raman spectroscopy provide important insights into allospecific immune responses generated after organ transplantation, and may be useful for therapeutic manipulation of the immune response
authors.affiliation: Kyung Hee University, Department of Biomedical Engineering and Healthcare Industry Research Institute, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Kyung Hee University, Department of Surgery, School of Medicine, Seoul 130-872, Republic of Korea.
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Ahn
authors.affiliation: Kyung Hee University, Department of Biomedical Engineering and Healthcare Industry Research Institute, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of KoreacGraduate School Kyung Hee University, Department of Medical Engineering, 1 Hoegi-dong, Don.
authors.forename: Gi-Ja
authors.initials: GJ
authors.lastname: Lee
authors.affiliation: Kyung Hee University, Department of Biomedical Engineering and Healthcare Industry Research Institute, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Gyeong Bok
authors.initials: GB
authors.lastname: Jung
authors.affiliation: Kyung Hee University, Department of Physiology, College of Korean Medicine, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Gihyun
authors.initials: G
authors.lastname: Lee
authors.affiliation: Myongji University, Department of Industrial and Management Engineering, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggi 449-72, Republic of Korea.
authors.forename: Dohyun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Kyung Hee University, Department of Ophthalmology, School of Medicine, Seoul 130-701, Republic of Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Kyung Hee University, Department of Ophthalmology, School of Medicine, Seoul 130-701, Republic of Korea.
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Jin
authors.affiliation: Kyung Hee University, Department of Biomedical Engineering and Healthcare Industry Research Institute, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of KoreacGraduate School Kyung Hee University, Department of Medical Engineering, 1 Hoegi-dong, Don.
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry* / Q000502:physiology; D002478:Cells, Cultured; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D057054:Molecular Imaging / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*