title: Functional effects of distinct innervation styles of pyramidal cells by fast spiking cortical interneurons.
abstract: Inhibitory interneurons target precise membrane regions on pyramidal cells, but differences in their functional effects on somata, dendrites and spines remain unclear. We analyzed inhibitory synaptic events induced by cortical, fast-spiking (FS) basket cells which innervate dendritic shafts and spines as well as pyramidal cell somata. Serial electron micrograph (EMg) reconstructions showed that somatic synapses were larger than dendritic contacts. Simulations with precise anatomical and physiological data reveal functional differences between different innervation styles. FS cell soma-targeting synapses initiate a strong, global inhibition, those on shafts inhibit more restricted dendritic zones, while synapses on spines may mediate a strictly local veto. Thus, FS cell synapses of different sizes and sites provide functionally diverse forms of pyramidal cell inhibition.
authors.affiliation: Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Yoshiyuki
authors.identifier: http://orcid.org/0000-0002-0950-7460
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kondo
authors.affiliation: Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Nomura
authors.affiliation: Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Sayuri
authors.initials: S
authors.lastname: Hatada
authors.affiliation: Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamaguchi
authors.affiliation: Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Alsayed A
authors.initials: AA
authors.lastname: Mohamed
authors.affiliation: Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Fuyuki
authors.initials: F
authors.lastname: Karube
authors.affiliation: Institute of Neuroscience and Medicine, Research Centre Jülich, Jülich, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Lübke
authors.affiliation: Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kawaguchi
mesh_terms: D000818:Animals; D066146:Cell Body / Q000648:ultrastructure; D002450:Cell Communication; D003712:Dendrites / Q000648:ultrastructure; D007395:Interneurons / Q000502:physiology*; D008854:Microscopy, Electron; D009433:Neural Inhibition; D017966:Pyramidal Cells / Q000502:physiology*; D017208:Rats, Wistar; D013569:Synapses / Q000648:ultrastructure; D059008:Time-Lapse Imaging
keywords: FS basket cell; IPSC; inhibitory synapse; neuroscience; pyramidal cell; rat; spine; synaptic conductance