title: Alginate-polyester comacromer based hydrogels as physiochemically and biologically favorable entities for cardiac tissue engineering.
abstract: The physiochemical and biological responses of tissue engineering hydrogels are crucial in determining their desired performance. A hybrid comacromer was synthesized by copolymerizing alginate and poly(mannitol fumarate-co-sebacate) (pFMSA). Three bimodal hydrogels pFMSA-AA, pFMSA-MA and pFMSA-NMBA were synthesized by crosslinking with Ca(2+) and vinyl monomers acrylic acid (AA), methacrylic acid (MA) and N,N'-methylene bisacrylamide (NMBA), respectively. Though all the hydrogels were cytocompatible and exhibited a normal cell cycle profile, pFMSA-AA exhibited superior physiochemical properties viz non-freezable water content (58.34%) and water absorption per unit mass (0.97 g water/g gel) and pore length (19.92±3.91 μm) in comparing with other two hydrogels. The increased non-freezable water content and water absorption of pFMSA-AA hydrogels greatly influenced its biological performance, which was evident from long-term viability assay and cell cycle proliferation. The physiochemical and biological favorability of pFMSA-AA hydrogels signifies its suitability for cardiac tissue engineering.
authors.affiliation: Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Thiruvananthapuram 695 012, Kerala State, India.
authors.forename: Finosh G
authors.initials: FG
authors.lastname: Thankam
authors.affiliation: Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Thiruvananthapuram 695 012, Kerala State, India. Electronic address: mjayabalan52@gmail.com.
authors.forename: Jayabalan
authors.initials: J
authors.lastname: Muthu
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002460:Cell Line; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000166:cytology*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D001798:Blood Proteins; D006603:Hexuronic Acids; D020100:Hydrogels; D011091:Polyesters; D020723:Glucuronic Acid
keywords: Cardiac tissue engineering; Cell cycle proliferation; Hybrid hydrogels; Long-term cell viability; Non-freezable water content