title: Six stroma-based RNA markers diagnostic for prostate cancer in European-Americans validated at the RNA and protein levels in patients in China.
abstract: We previously analyzed human prostate tissue containing stroma near to tumor and from cancer-negative tissues of volunteers. Over 100 candidate gene expression differences were identified and used to develop a classifier that could detect nearby tumor with an accuracy of 97% (sensitivity = 98% and specificity = 88%) based on 364 independent test cases from primarily European American cases. These stroma-based gene signatures have the potential to identify cancer patients among those with negative biopsies. In this study, we used prostate tissues from Chinese cases to validate six of these markers (CAV1, COL4A2, HSPB1, ITGB3, MAP1A and MCAM). In validation by real-time PCR, four genes (COL4A2, HSPB1, ITGB3, and MAP1A) demonstrated significantly lower expression in tumor-adjacent stroma compared to normal stroma (p value ≤ 0.05). Next, we tested whether these expression differences could be extended to the protein level. In IHC assays, all six selected proteins showed lower expression in tumor-adjacent stroma compared to the normal stroma, of which COL4A2, HSPB1 and ITGB3 showed significant differences (p value ≤ 0.05). These results suggest that biomarkers for diagnosing prostate cancer based on tumor microenvironment may be applicable across multiple racial groups.
authors.affiliation: Guizhou Provincial Key Laboratory of Computational Nano-Material Science, Guizhou Normal College, Guiyang, China.|Department of Urology, Guizhou Provincial People's Hospital, Guizhou, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Guizhou Provincial Key Laboratory of Computational Nano-Material Science, Guizhou Normal College, Guiyang, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Chemical Physics, University of Science and Technology of China, Hefei, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Guizhou Provincial Key Laboratory of Computational Nano-Material Science, Guizhou Normal College, Guiyang, China.
authors.forename: Mingsen
authors.initials: M
authors.lastname: Deng
authors.affiliation: National Engineering Center for Biochip at Shanghai, Shanghai, China.
authors.forename: Hengjun
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA, USA.
authors.forename: Bozhao
authors.initials: B
authors.lastname: Men
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: McClelland
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Irvine, Irvine, CA, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Mercola
authors.affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Wei-D
authors.initials: WD
authors.lastname: Zhong
authors.affiliation: Guizhou Provincial Key Laboratory of Computational Nano-Material Science, Guizhou Normal College, Guiyang, China.|Department of Statistics, The University of Akron, Akron, OH, USA.|Department of Family and Community Medicine, Northeast Ohio Medical University, Rootstown, OH, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jia
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002681:China; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D059016:Tumor Microenvironment; D044465:White People / Q000235:genetics; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor
keywords: diagnosis; microenvironment; prostate cancer; race; stroma