title: The Effects of Platelet-Rich Plasma on Halting the Progression in Porcine Intervertebral Disc Degeneration.
abstract: Disc degeneration and the subsequent herniation and/or rupture of the intervertebral disc (IVD) are due to a failure of the extracellular matrix of the annulus to contain the contents of the nucleus. This results from inadequate maintenance of the matrix components as well as the proteolytic activity of matrix metalloproteinases (MMPs) that degrade matrix molecules. Arresting progression of disc degeneration in the annulus holds greater clinical potential at this point than prevention of its onset in the nucleus. Therefore, in this study, we have therapeutic aims that would decrease levels of the cytokines and growth factors that indirectly lead to disc degeneration via stimulating MMP and increase levels of several beneficial growth factors, such as transforming growth factor-β, with the addition of platelet-rich plasma (PRP) that would stimulate cell growth and matrix synthesis. For this study, we attempted to address these imbalances of metabolism by using tumor necrosis factor-α treated annulus fibrosus cells isolated from porcine IVD tissue and incubating the cells in a growth factor rich environment with PRP. These results indicate that the PRP in vitro increased the production of the major matrix components (type II collagen and aggrecan) and decreased the inhibitory collagenase MMP-1. This application will address a therapeutic approach for intervening early in the degenerative process.
authors.affiliation: Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center, Memphis, TN, USA.|Veterans Affairs Medical Center, Memphis, TN, USA.
authors.forename: Hongsik
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Holt
authors.affiliation: Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center, Memphis, TN, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Smith
authors.affiliation: Department of Biological Sciences, Kongju National University, Gongju-si, South Korea.
authors.forename: Song-Ja
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Campbell Clinic, Memphis, TN, USA.
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Gardocki
authors.affiliation: Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center, Memphis, TN, USA.|Veterans Affairs Medical Center, Memphis, TN, USA.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Hasty
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D007403:Intervertebral Disc / Q000378:metabolism / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013552:Swine
chemical_list: D020782:Matrix Metalloproteinases
keywords: Annulus fibrosus; Degeneration; Intervertebral disc; Matrix metalloproteinases; Platelet rich plasma