title: The influence of number of counts in the myocardium in the determination of reproducible functional parameters in gated-SPECT studies simulated with GATE.
abstract: Myocardial perfusion gated-single photon emission computed tomography (gated-SPECT) imaging is used for the combined evaluation of myocardial perfusion and left ventricular (LV) function. The aim of this study is to analyze the influence of counts/pixel and concomitantly the total counts in the myocardium for the calculation of myocardial functional parameters.
  
  
  METHODS
  Gated-SPECT studies were performed using a Monte Carlo GATE simulation package and the NCAT phantom. The simulations of these studies use the radiopharmaceutical (99m)Tc-labeled tracers (250, 350, 450 and 680 MBq) for standard patient types, effectively corresponding to the following activities of myocardium: 3, 4.2, 5.4-8.2 MBq. All studies were simulated using 15 and 30s/projection. The simulated data were reconstructed and processed by quantitative-gated-SPECT software, and the analysis of functional parameters in gated-SPECT images was done by using Bland-Altman test and Mann-Whitney-Wilcoxon test.
  
  
  RESULTS
  In studies simulated using different times (15 and 30s/projection), it was noted that for the activities for full body: 250 and 350 MBq, there were statistically significant differences in parameters Motility and Thickness. For the left ventricular ejection fractio n (LVEF), end-systolic volume (ESV) it was only for 250 MBq, and 350 MBq in the end-diastolic volume (EDV), while the simulated studies with 450 and 680MBq showed no statistically significant differences for global functional parameters: LVEF, EDV and ESV.
  
  
  CONCLUSIONS
  The number of counts/pixel and, concomitantly, the total counts per simulation do not significantly interfere with the determination of gated-SPECT functional parameters, when using the administered average activity of 450 MBq, corresponding to the 5.4 MBq of the myocardium, for standard patient types.
authors.affiliation: Instituto de Biofísica e Engenharia Biomédica, Faculdade de Ciências, Universidade de Lisboa, Portugal; Área Científica de Medicina Nuclear, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Portugal. Electronic address: vieira.lina@sapo.pt.
authors.forename: L
authors.initials: L
authors.lastname: Vieira
authors.affiliation: HPP Medicina Molecular, SA, Porto, Portugal; Medicina Nuclear/Radiofarmacologia - Fundação Champalimaus, Lisboa, Portugal.
authors.forename: D C
authors.initials: DC
authors.lastname: Costa
authors.affiliation: Instituto de Biofísica e Engenharia Biomédica, Faculdade de Ciências, Universidade de Lisboa, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Almeida
mesh_terms: D055426:Cardiac-Gated Single-Photon Emission Computer-Assisted Tomography / Q000379:methods*; D003198:Computer Simulation; D006334:Heart Function Tests / Q000295:instrumentation / Q000379:methods; D007091:Image Processing, Computer-Assisted; D008955:Models, Cardiovascular; D009010:Monte Carlo Method; D055414:Myocardial Perfusion Imaging / Q000379:methods*; D019047:Phantoms, Imaging; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D012984:Software; D013667:Technetium; D016277:Ventricular Function, Left
chemical_list: D019275:Radiopharmaceuticals; D013667:Technetium
keywords: Functional parameters; GATE; Gated-SPECT; NCAT; Number of counts; Número de cuentas; Parámetros funcionales