title: [The Relationship Study between Expressions of P2X5 Receptor and Deficiency-cold Syndrome/Deficiency-heat Syndrome at Various Ambient Temperatures].
abstract: OBJECTIVE
  To detect the expression of the peripheral blood P2X5 receptor at various ambient temperatures, and to explore its relationship with deficiency-cold syndrome and deficiency-heat syndrome.
  
  
  METHODS
  Subjects were selected by questionnaire and expert diagnosis, and assigned to the normal control group, the deficiency-cold syndrome group, and the deficiency-heat syndrome group, 20 in each group. 5 mL venous blood was collected at room temperature (25 °C) and cold temperature (-4-5 °C) respectively. Then the expression of P2X5 receptor was relatively quantified by real-time fluorescence quantitative PCR, and compared at room temperature and cold temperature respectively.
  
  
  RESULTS
  The expression of P2X5 receptor in deficiency-cold syndrome and deficiency-heat syndrome groups was lower than that in the normal control group at room temperature (P < 0.05). It decreased more at cold temperature in the deficiency-cold syndrome group than in the normal control group (P < 0.01) as well as in the deficiency-heat syndrome group (P < 0.05). The expression of P2X5 receptor showed no difference in all groups at two different temperatures (P > 0.05).
  
  
  CONCLUSIONS
  The expression of P2X5 receptor was different in different syndrome groups at various ambient temperatures. Ambient temperatures had insignificant effect on the expression of P2X5 receptor of the population with the same syndrome.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Yang
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Yu
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Xin-min
authors.initials: XM
authors.lastname: Li
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: Zhan
authors.forename: Jun-lin
authors.initials: JL
authors.lastname: Hou
mesh_terms: D003080:Cold Temperature; D006358:Hot Temperature; D006801:Humans; D008516:Medicine, Chinese Traditional; D058485:Receptors, Purinergic P2X5 / Q000378:metabolism*; D013577:Syndrome
chemical_list: D058485:Receptors, Purinergic P2X5