title: Molecular cloning and sequencing analysis of the interferon β from Coturnix.
abstract: One pair of primers was designed according to Gallus and Meleagris gallopavo interferon β (IFN-β) sequences published in GenBank. The primers and RNA extraction from the spleen of Coturnix were used to amplify Coturnix IFN-β cDNA by real-time polymerase chain reaction (RT-PCR). The product was cloned into pEasy-T1 vector. Evaluating recombinant plasmid by PCR and restriction enzyme digestion. Sequence the cloning sequences, comparing the sequencing results by NCBI. We successfully got a Coturnix IFN-β partial sequence. The sequence was subtyped and put to homologous analysis. The results suggested the homology of IFN-β gene of Coturnix and gene of Coturnix and chicken (88.7%), the homology of IFN-β gene of Coturnix and chicken (88.7%), the homology of IFN-β gene of Coturnix and Anas platyrhynchos (72.5%), the homology of IFN-β sequence registered in GenBank. The analysis of the genetic tree showed that the relationship of Coturnix and chicken IFN-β had a high homology. It can be seen that in this study we successfully got a partial sequence of IFN-β of quail.
authors.affiliation: Shandong Agriculture University, Taian, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Shandong Agriculture University, Taian, PR China.
authors.forename: Wei-Shan
authors.initials: WS
authors.lastname: Chang
keywords: IFN-β; coturnix; gene cloning; gene sequence analysis