title: RPS24 knockdown inhibits colorectal cancer cell migration and proliferation in vitro.
abstract: Besides new proteins synthesis, ribosomal protein has a role in extra-ribosomal functions, which are related to many diseases, such as Diamond-Blackfan anemia, hypoplasia, and cell apoptosis. However, the importance of RPS24 in human colon cancer is largely unknown. In this study, RPS24 gene expression was significantly inhibited in human colon cancer HCT116 and HT-29 cells using a lentivirus shRNA approach. Knockdown of RPS24 expression significantly inhibited cell proliferation, colony formation, cell migration and arrested cell in S phase. The results demonstrated for the first time that RPS24 gene had a critical role in human colon cancer. Therefore, our findings indicated that RPS24 gene may be a promising biomarker for therapy in human colon cancer and may have a potential application in the diagnosis or treatment of human colon cancer.
authors.affiliation: Department of General Surgery, 309 Hospital of People's Liberation Army, 17 Heishanhu Road, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, 168 Changhai Road, Shanghai, China.
authors.forename: Jinke
authors.initials: J
authors.lastname: Sui
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, 168 Changhai Road, Shanghai, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, 168 Changhai Road, Shanghai, China.
authors.forename: Fuao
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, 168 Changhai Road, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Department of General Surgery, 309 Hospital of People's Liberation Army, 17 Heishanhu Road, Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Colorectal Surgery, Changhai Hospital, 168 Changhai Road, Shanghai, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, 309 Hospital of People's Liberation Army, 17 Heishanhu Road, Beijing, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of General Surgery, 309 Hospital of People's Liberation Army, 17 Heishanhu Road, Beijing, China. Electronic address: ydpu2000@hotmail.com.
authors.forename: Y D
authors.initials: YD
authors.lastname: Pu
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C489053:RPS24 protein, human; D012269:Ribosomal Proteins
keywords: Cell cycle; Colon cancer; Migration; PS24; Proliferation