title: Protective effects of aloperine on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion.
abstract: Aloperine (ALO), one of the alkaloids isolated from Sophora alopecuroides L., is traditionally used for various diseases including neuronal disorders. This study investigated the protective effects of ALO on neonatal rat primary-cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion (OGD/RP). Treatment with ALO (25, 50, and 100 mg/l) attenuated neuronal damage (p < 0.01), with evidence of increased cell viability (p < 0.01) and decreased cell morphologic impairment. Furthermore, ALO increased mitochondrial membrane potential (p < 0.01), but inhibited intracellular-free calcium [Ca(2+)] i (p  < 0.01) elevation in a dose-dependent manner at OGD/RP. ALO also reduced the intracellular reactive oxygen species and malondialdehyde production and enhanced the antioxidant enzymatic activities of catalase, superoxide dismutase, glutathione peroxidase and the total antioxidant capacity. The results suggested that ALO has significant neuroprotective effects that can be attributed to anti-oxidative stress.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, 750004, China.
authors.forename: Ning-Tian
authors.initials: NT
authors.lastname: Ma
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
authors.forename: Ren-Yuan
authors.initials: RY
authors.lastname: Chang
authors.forename: Yin-Ju
authors.initials: YJ
authors.lastname: Hao
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Shao-Ju
authors.initials: SJ
authors.lastname: Jin
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Niu
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Yu
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D005947:Glucose / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D010100:Oxygen; D010880:Piperidines / Q000008:administration & dosage / Q000627:therapeutic use*; D054837:Quinolizidines; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D018696:Neuroprotective Agents; D010880:Piperidines; D054837:Quinolizidines; C062701:aloperine; D005947:Glucose; D010100:Oxygen