title: Three Dimensional Nano "Langmuir Trough" for Lipid Studies.
abstract: A three-dimensional-phospholipid monolayer with tunable molecular structure was created on the surface of oil nanodroplets from a mixture of phospholipids, oil, and water. This simple nanoemulsion preparation technique generates an in situ prepared membrane model system with controllable molecular surface properties that resembles a lipid droplet. The molecular interfacial structure of such a nanoscopic system composed of hexadecane, 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC), and water was determined using vibrational sum frequency scattering and second harmonic scattering techniques. The droplet surface structure of DPPC can be tuned from a tightly packed liquid condensed phase like monolayer to a more dilute one that resembles the liquid condensed/liquid expanded coexistence phase by varying the DPPC/oil/water ratio. The tunability of the chemical structure, the high surface-to-volume ratio, and the small sample volume make this system an ideal model membrane for biochemical research.
authors.affiliation: †Laboratory for Fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), School of Engineering (STI), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
authors.forename: Yixing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: †Laboratory for Fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), School of Engineering (STI), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.|‡Department of Physics, Indian Institute of Technology Ropar, Rupnagar, 140001, India.
authors.forename: Kailash C
authors.initials: KC
authors.lastname: Jena
authors.affiliation: †Laboratory for Fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), School of Engineering (STI), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
authors.forename: Cornelis
authors.initials: C
authors.lastname: Lütgebaucks
authors.affiliation: †Laboratory for Fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), School of Engineering (STI), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
authors.forename: Halil I
authors.initials: HI
authors.lastname: Okur
authors.affiliation: †Laboratory for Fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), School of Engineering (STI), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Roke
mesh_terms: D000473:Alkanes / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000473:Alkanes; D004655:Emulsions; D009821:Oils; D010743:Phospholipids; D014867:Water; C007932:n-hexadecane
keywords: Langmuir monolayers; Lipids; emulsion; lipid droplets; second harmonic generation; sum frequency generation