title: Supramolecular interaction of gemifloxacin and hydroxyl propyl β-cyclodextrin spectroscopic characterization, molecular modeling and analytical application.
abstract: The solid inclusion complex of gemifloxacin (GFX) and hydroxyl propyl β-cyclodextrin (HPβ-CD) was prepared and examined by UV-visible, FTIR, NMR, electrospray ionization mass spectrometry (ESI-MS) and fluorescence spectroscopy. The formation of inclusion complex has been confirmed on the basis of changes of spectroscopic properties. Further the interaction between GFX and HPβ-CD was studied using molecular modeling approaches. The results showed that HPβCD reacted with GFX to form a 1:1 host-guest inclusion complex. Based on the enhancement of the fluorescence intensity of GFX produced through complex formation, a simple, accurate, rapid and highly sensitive spectrofluorometric method for the determination of GFX in pharmaceutical formulation was developed. The linear relationships between the intensity and GFX concentration was obtained in the concentration range of 20-140 ng/mL with good correlation coefficients (0.9997). The limit of detection (LOD) was found to be 4 ng/mL. The proposed method was successfully applied to the analysis of GFX in pharmaceutical preparation.
authors.affiliation: University of Khartoum, Faculty of Science, Chemistry Department, Khartoum 11115, Sudan.
authors.forename: Nuha Fathi Ali
authors.initials: NF
authors.lastname: Dsugi
authors.affiliation: University of Khartoum, Faculty of Science, Chemistry Department, Khartoum 11115, Sudan. Electronic address: aaelbashir@uofk.edu.
authors.forename: Abdalla A
authors.initials: AA
authors.lastname: Elbashir
authors.affiliation: Department of Chemistry, College of Science, Sultan Qaboos University, Al-Khod 123, Oman.
authors.forename: Fakhr Eldin Osman
authors.initials: FE
authors.lastname: Suliman
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D005079:Excipients / Q000737:chemistry / Q000378:metabolism; D024841:Fluoroquinolones / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D000077735:Gemifloxacin; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D009287:Naphthyridines / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D059005:Topoisomerase II Inhibitors / Q000737:chemistry / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005079:Excipients; D024841:Fluoroquinolones; D009287:Naphthyridines; D059005:Topoisomerase II Inhibitors; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000077735:Gemifloxacin
keywords: Gemifloxacin; HPβCD; Inclusion complex; Molecular modeling; Supramolecular