title: Angiotensin II receptor antagonist olmesartan and NF-kappaB inhibitor as cytotoxic and apoptotic agents in MCF-7 human cell line.
abstract: OBJECTIVE
  Over expression of renin-angiotensin system (RAS) and nuclear factor-kappaB (NF-kappaB) have major role in many cancers. In this study, role of angiotensin II (Ag II) and NF-kappaB pathway in breast cancer cell line (MCF-7) proliferation were studied using olmesartan (as a novel Ag II antagonist) and Bay11-7082 (as NF-kappaB inhibitor).
  
  
  METHODS
  Cells were treated with different concentrations of olmesartan and Bay11-7082.Cell proliferation was determined by 4, 5-Dimethylthiazol-2-yl, 2, 5-diphenyl tetrazolium (MTT) assay. Apoptotic cells were evaluated using PI staining of DNA fragmentation.
  
  
  RESULTS
  Olmesartan and Bay11-7082 decreased cell viability. Combination of olmesartan with Bay11-7082 also decreased cell viability as compared with single agent treatments. Results showed that apoptosis is involved in olmesartan and Bay11-7082-induced toxicity.
  
  
  CONCLUSIONS
  Olmesartan and Bay11-7082 inhibit the MCF-7 cells growth indicating RAS and NF-kappaB pathway blockade lead to cytotoxicity and apoptosis induction against tumour cells. So ARBs and NF-kappaB pathway inhibitors could be considered as anticancer drugs in future.
authors.affiliation: a Department of Pharmacology, School of Medicine , Mashhad University of Medical Sciences , Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Bakhtiari
authors.affiliation: b Pharmacological Research Center of Medicinal Plants, School of Medicine , Mashhad University of Medical Sciences , Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Hosseini
authors.affiliation: a Department of Pharmacology, School of Medicine , Mashhad University of Medical Sciences , Iran.|b Pharmacological Research Center of Medicinal Plants, School of Medicine , Mashhad University of Medical Sciences , Iran.
authors.forename: Mohammad Taher
authors.initials: MT
authors.lastname: Boroushaki
authors.affiliation: a Department of Pharmacology, School of Medicine , Mashhad University of Medical Sciences , Iran.|b Pharmacological Research Center of Medicinal Plants, School of Medicine , Mashhad University of Medical Sciences , Iran.|c Medical Toxicology Research Center, School of Medicine , Mashhad University of Medical Sciences , Iran.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Mousavi
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000633:toxicity*; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007093:Imidazoles / Q000633:toxicity*; D061986:MCF-7 Cells; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009570:Nitriles / Q000633:toxicity*; D013450:Sulfones / Q000633:toxicity*; D013777:Tetrazoles / Q000633:toxicity*
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D047228:Angiotensin II Type 1 Receptor Blockers; D000970:Antineoplastic Agents; D007093:Imidazoles; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; D013777:Tetrazoles; C437965:olmesartan
keywords: Bay11-7082; Cytotoxicity; MCF-7; Olmesartan