title: MicroRNA-183 increases osteoclastogenesis by repressing heme oxygenase-1.
abstract: Emerging evidence suggests that microRNAs (miRs) influence skeletal structure by modulating osteoclastogenesis and bone resorption. We have demonstrated previously that the up-regulation of heme oxygenase-1 (HO-1) attenuated osteoclastogenesis in bone marrow-derived macrophages (BMMs). RANKL-induced osteoclastogenesis elevates microRNA-183 (miR-183) in BMM. We show here that HO-1 is a target gene of miR-183 and that this miRNA binds to the 3'-UTR of HO-1. We find that a synthetic inhibitor that binds to miR-183 decreases osteoclast (OC) differentiation and increases the expression of HO-1, while a mimic of endogenous mature miR-183 has the opposite effect. Moreover, the HO-1 inducers, resveratrol and piceatannol decrease the expression of miR-183, resulting in attenuated osteoclastogenesis. Our findings reveal how miR-183 affects OC formation.
authors.affiliation: Department of Biological Sciences (BK21 Program), University of Ulsan, Ulsan 680-749, South Korea.
authors.forename: Ke
authors.initials: K
authors.lastname: Ke
authors.affiliation: Department of Biological Sciences (BK21 Program), University of Ulsan, Ulsan 680-749, South Korea.
authors.forename: Ok-Joo
authors.initials: OJ
authors.lastname: Sul
authors.affiliation: Department of Biological Sciences (BK21 Program), University of Ulsan, Ulsan 680-749, South Korea.
authors.forename: Monisha
authors.initials: M
authors.lastname: Rajasekaran
authors.affiliation: Department of Biological Sciences (BK21 Program), University of Ulsan, Ulsan 680-749, South Korea. Electronic address: hschoi@mail.ulsan.ac.kr.
authors.forename: Hye-Seon
authors.initials: HS
authors.lastname: Choi
mesh_terms: D000818:Animals; D002460:Cell Line; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis*; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D053245:RANK Ligand / Q000494:pharmacology
chemical_list: D035683:MicroRNAs; C543802:Mirn183 microRNA, mouse; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D051547:Heme Oxygenase-1
keywords: Heme oxygenase-1; Osteoclast; Piceatannol; Resveratrol; miR-183