title: Double and zero quantum filtered (2)H NMR analysis of D2O in intervertebral disc tissue.
abstract: The analysis of double and zero quantum filtered (2)H NMR spectra obtained from D2O perfused in the nucleus pulposus of human intervertebral disc tissue samples is reported. Fitting the spectra with a three-site model allows for residual quadrupolar couplings and T2 relaxation times to be measured. The analysis reveals changes in both the couplings and relaxation times as the tissue begins to show signs of degradation. The full analysis demonstrates that information about tissue hydration, water collagen interactions, and sample heterogeneity can be obtained and used to better understand the biochemical differences between healthy and degraded tissue.
authors.affiliation: Department of Chemistry, The King's University, Edmonton, Alberta, Canada. Electronic address: kris.ooms@kingsu.ca.
authors.forename: Kristopher J
authors.initials: KJ
authors.lastname: Ooms
authors.affiliation: Brown Laboratories, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Vega
authors.affiliation: Brown Laboratories, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Polenova
authors.affiliation: Department of Materials Science and Engineering, Drexel University, Philadelphia, PA, USA.
authors.forename: Marco
authors.initials: M
authors.lastname: Cannella
authors.affiliation: Department of Materials Science and Engineering, Drexel University, Philadelphia, PA, USA.
authors.forename: Michele
authors.initials: M
authors.lastname: Marcolongo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000465:Algorithms; D001834:Body Water / Q000737:chemistry*; D017666:Deuterium Oxide / Q000032:analysis*; D006801:Humans; D006859:Hydrogen / Q000032:analysis; D007403:Intervertebral Disc / Q000737:chemistry*; D007405:Intervertebral Disc Displacement / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D055815:Young Adult
chemical_list: D006859:Hydrogen; D017666:Deuterium Oxide
keywords: Collagen hydration; DQF/ZQF NMR; Degenerative disc disease; Residual quadrupolar couplings; Spinal disc tissue; T(2) relaxation