title: Asymmetric synthesis of optically active methyl-2-benzamido-methyl-3-hydroxy-butyrate by robust short-chain alcohol dehydrogenases from Burkholderia gladioli.
abstract: Three short-chain alcohol dehydrogenases from Burkholderia gladioli were discovered for their great potential in the dynamic kinetic asymmetric transformation of methyl 2-benzamido-methyl-3-oxobutanoate, and their screening against varied organic solvents and substrates. This is the first report of recombinant enzymes capable of achieving this reaction with the highest enantio- and diastereo-selectivity.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China. zhengyg@zjut.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Huang
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Lin
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D001549:Benzamides / Q000737:chemistry / Q000378:metabolism*; D042725:Burkholderia gladioli / Q000201:enzymology*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D055620:Optical Phenomena
chemical_list: D001549:Benzamides; D006885:Hydroxybutyrates; D000426:Alcohol Dehydrogenase