title: LCN2 Promoter Methylation Status as Novel Predictive Marker for Microvessel Density and Aggressive Tumor Phenotype in Breast Cancer Patients.
abstract: LCN2 (Lipocalin 2) is a 25 KD secreted acute phase protein, reported to be a novel regulator of angiogenesis in breast cancer. Up regulation of LCN2 had been observed in multiple cancers including breast cancer, pancreatic cancer and ovarian cancer. However, the role of LCN2 promoter methylation in the formation of microvessels is poorly understood. The aim of this study was to analyze the association of LCN 2 promoter methylation with microvessel formation and tumor cell proliferation in breast cancer patients. The LCN2 promoter methylation status was studied in 64 breast cancer tumors by methylation specific PCR (MSP). Evaluation of microvessel density (MVD) and Ki67 cell proliferation index was achieved by immunohistochemical staining using CD34 and MIB-1 antibodies, respectively. LCN2 promoter unmethylation status was observed in 43 (67.2%) of breast cancer patients whereas LCN2 methylation status was seen in 21 (32.8%). Further, LCN2 promoter unmethylation status was associated with aggressive tumor phenotype and elevated mean MVD in breast cancer patients.
authors.affiliation: Department of Genetics, Osmania University, Kanchanbagh, India E-mail : sattivishnupriya@gmail.com.
authors.forename: Phanni bhushann
authors.initials: Pb
authors.lastname: Meka
authors.forename: Sarika
authors.initials: S
authors.lastname: Jarjapu
authors.forename: Santhoshi Rani
authors.initials: SR
authors.lastname: Nanchari
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Vishwakarma
authors.forename: Prajitha Mohandas
authors.initials: PM
authors.lastname: Edathara
authors.forename: Manjula
authors.initials: M
authors.lastname: Gorre
authors.forename: Anuradha
authors.initials: A
authors.lastname: Cingeetham
authors.forename: Sugunakar
authors.initials: S
authors.lastname: Vuree
authors.forename: Sandhya
authors.initials: S
authors.lastname: Annamaneni
authors.forename: Nageswara Rao
authors.initials: NR
authors.lastname: Dunna
authors.forename: Srinivasulu
authors.initials: S
authors.lastname: Mukta
authors.forename: Triveni
authors.initials: T
authors.lastname: B
authors.forename: Vishnupriya
authors.initials: V
authors.lastname: Satti
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics*; D000328:Adult; D000974:Antibodies, Antinuclear / Q000235:genetics; D000911:Antibodies, Monoclonal / Q000235:genetics; D018952:Antigens, CD34 / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics; D000071068:Lipocalin-2; D054834:Lipocalins / Q000235:genetics*; D055806:Microvessels / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000209:Acute-Phase Proteins; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; C440408:MIB-1 antibody; D011518:Proto-Oncogene Proteins