title: Plasmid borne BAC-IB17: Localization of a potential antibacterial positive marker (Bac+) encoded broad inhibitory spectrum bacteriocin.
abstract: Production of antimicrobial compounds is considered as ubiquitous anti-competitor strategy in bacterial ecosystem. Bacteriocins are heterogeneous; highly specific and efficient anti-competitor agents and the gene responsible for the production of bacteriocins mostly exist in an autosomal state and associated with plasmids. BAC-IB17 is a broad spectrum bacteriocin and its production was observed at different stages of the growth cycle from Bacillus subtilis KIBGE-IB17. Growth kinetics of B. subtilis KIBGE-IB17 along with the production of BAC-IB17 showed that it exhibited secondary metabolite kinetics. Plasmid curing technique revealed that the gene responsible for the bacteriocinogenecity in B. subtilis KIBGE-IB17 was located on the plasmid of the bacterium. Overlay method also demonstrated the plasmid-mediated bacteriocinogenesis of the isolated colonies. With the advancement in genomics and proteomics, the plasmid borne BAC-IB17 can play a significant role in the transfer of bacteriocinogenic factor to other incompetent cells and also in the maintenance of plasmid in bacterial population.
authors.affiliation: The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Asma
authors.initials: A
authors.lastname: Ansari
authors.affiliation: The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Nadir Naveed
authors.initials: NN
authors.lastname: Siddiqui
authors.affiliation: The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Maria
authors.initials: M
authors.lastname: Ghani
authors.affiliation: The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.affiliation: The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Shah Aliul
authors.initials: SA
authors.lastname: Qader
mesh_terms: D000900:Anti-Bacterial Agents; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins