title: Nuclear versus cytosolic activity of the yeast Hog1 MAP kinase in response to osmotic and tunicamycin-induced ER stress.
abstract: We examined the physiological significance of the nuclear versus cytosolic localization of the MAPK Hog1p in the ability of yeast cells to cope with osmotic and ER (endoplasmic reticulum) stress. Our results indicate that nuclear import of Hog1p is not critical for osmoadaptation. Plasma membrane-anchored Hog1p is still able to induce increased expression of GPD1 and glycerol accumulation. This is a key osmoregulatory event, although a small production of the osmolyte coupled with the nuclear import of Hog1p is sufficient to provide osmoresistance. On the contrary, the nuclear activity of Hog1p is dispensable for ER stress adaptation.
authors.affiliation: Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Agustín Escardino, 7, 46980 Paterna, Valencia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: García-Marqués
authors.affiliation: Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Agustín Escardino, 7, 46980 Paterna, Valencia, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Randez-Gil
authors.affiliation: Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Agustín Escardino, 7, 46980 Paterna, Valencia, Spain. Electronic address: prieto@iata.csic.es.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Prieto
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000222:Adaptation, Physiological; D000890:Anti-Infective Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D050505:Mutant Proteins / Q000378:metabolism; D009994:Osmolar Concentration; D064587:Osmoregulation; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012462:Saline Solution, Hypertonic / Q000737:chemistry; D013012:Sorbitol / Q000737:chemistry; D014415:Tunicamycin / Q000494:pharmacology; D056811:Unfolded Protein Response / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D050505:Mutant Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D012462:Saline Solution, Hypertonic; D014415:Tunicamycin; D049452:Green Fluorescent Proteins; D013012:Sorbitol; C568531:GPD1 protein, S cerevisiae; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases; D005990:Glycerol
keywords: GPD1,2; Glycerol; HOG pathway; Osmotolerance; Unfolded proteins stress