title: Clinical Usefulness of Immunohistochemical Staining of p57 kip2 for the Differential Diagnosis of Complete Mole.
abstract: OBJECTIVE
  Can polymer-based immunohistochemical staining of p57(kip2) replace DNA analysis as an inexpensive means of differentiating complete mole from partial mole or hydropic abortion?
  
  
  METHODS
  Original paraffin-embedded tissue blocks from 14 equivocal cases were turned over to our laboratory and examined by immunohistochemical staining of p57(kip2).
  
  
  RESULTS
  Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining. The remaining 10 cases showed apparently positive staining in cytotrophoblasts and villous stromal cells. Without DNA analysis we are able to clearly differentiate the 4 cases of complete mole among the 14 equivocal cases. During follow-up, secondary low-risk gestational trophoblastic neoplasia (GTN) developed in 1 of the 4 cases of complete mole: the GTN was treated by single-agent chemotherapy. No subsequent changes were observed during follow-up in the other cases.
  
  
  CONCLUSIONS
  Polymer-based immunohistochemical staining of p57(kip2) (paternally imprinted gene, expressed from maternal allele) is a very effective method that can be used to differentiate androgenetic complete mole from partial mole and hydropic abortion. We might be able to avoid the cost of DNA analysis.
authors.affiliation: Department of Obstetrics and Gynecology, Iino Hospital, 4-3-2 Fuda, Chōfu, Tokyo 182-0024, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sasaki
authors.affiliation: Department of Obstetrics and Gynecology, Showa University Northern Yokohama Hospital and Showa University School of Medicine, 35-1 Chigasaki Chuo, Tsuduki-Ku, Yokohama, Kanagawa 224-8503, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sasaki
authors.affiliation: Department of Pathology, Showa University School of Medicine and Showa University Northern Yokohama Hospital, 35-1 Chigasaki Chuo, Tsuduki-Ku, Yokohama, Kanagawa 224-8503, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kunimura
authors.affiliation: Department of Obstetrics and Gynecology, Showa University Northern Yokohama Hospital and Showa University School of Medicine, 35-1 Chigasaki Chuo, Tsuduki-Ku, Yokohama, Kanagawa 224-8503, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
authors.affiliation: Maternity Clinic Kojima, 4-1-27 Asahi-Cho, Akishima, Tokyo 196-0025, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kojima
authors.affiliation: Department of Obstetrics and Gynecology, Iino Hospital, 4-3-2 Fuda, Chōfu, Tokyo 182-0024, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Iino
mesh_terms: D000022:Abortion, Spontaneous / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D000328:Adult; D000818:Animals; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D005260:Female; D031901:Gestational Trophoblastic Disease / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D007150:Immunohistochemistry; D008875:Middle Aged; D011247:Pregnancy
chemical_list: D050761:Cyclin-Dependent Kinase Inhibitor p57