title: Biochemical Modulation of Lipid Pathway in Microalgae Dunaliella sp. for Biodiesel Production.
abstract: Exploitation of renewable sources of energy such as algal biodiesel could turn energy supplies problem around. Studies on a locally isolated strain of Dunaliella sp. showed that the mean lipid content in cultures enriched by 200 mg L(-1) myoinositol was raised by around 33% (1.5 times higher than the control). Similarly, higher lipid productivity values were achieved in cultures treated by 100 and 200 mg L(-1) myoinositol. Fluorometry analyses (microplate fluorescence and flow cytometry) revealed increased oil accumulation in the Nile red-stained algal samples. Moreover, it was predicted that biodiesel produced from myoinositol-treated cells possessed improved oxidative stability, cetane number, and cloud point values. From the genomic point of view, real-time analyses revealed that myoinositol negatively influenced transcript abundance of AccD gene (one of the key genes involved in lipid production pathway) due to feedback inhibition and that its positive effect must have been exerted through other genes. The findings of the current research are not to interprete that myoinositol supplementation could answer all the challenges faced in microalgal biodiesel production but instead to show that "there is a there there" for biochemical modulation strategies, which we achieved, increased algal oil quantity and enhanced resultant biodiesel quality.
authors.affiliation: Microbial Biotechnology Department, Semnan University, Semnan 35131-19111, Iran.
authors.forename: Ahmad Farhad
authors.initials: AF
authors.lastname: Talebi
authors.affiliation: Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran ; Biofuel Research Team (BRTeam), Karaj 31438-44693, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Tohidfar
authors.affiliation: Genomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran.
authors.forename: Seyedeh Mahsa
authors.initials: SM
authors.lastname: Mousavi Derazmahalleh
authors.affiliation: Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran ; Tropical Agro-Biomass (TAB) Group, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Malaysia.
authors.forename: Alawi
authors.initials: A
authors.lastname: Sulaiman
authors.affiliation: Faculty of Engineering, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia.
authors.forename: Azhari Samsu
authors.initials: AS
authors.lastname: Baharuddin
authors.affiliation: Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran ; Biofuel Research Team (BRTeam), Karaj 31438-44693, Iran.
authors.forename: Meisam
authors.initials: M
authors.lastname: Tabatabaei
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids