title: Enhanced daidzin production from jasmonic and acetyl salicylic acid elicited hairy root cultures of Psoralea corylifolia L. (Fabaceae).
abstract: Daidzin (7-O-glucoside of daidzein) has several pharmacological benefits in herbal remedy, as antioxidant and shown antidipsotropic activity. Hairy root culture of Psoralea corylifolia L. was developed for biomass and enhanced daidzin production using signalling compounds such as jasmonic acid (JA) and acetyl salicylic acid (ASA). Best response of 2.8-fold daidzin (5.09% DW) with 1 μM JA treatment after second week and 7.3-fold (3.43% DW) with 10 μM JA elicitation after 10th week was obtained from hairy roots compared to untreated control. ASA at 10 μM promoted 1.7-fold increase in daidzin (1.49% DW) content after seventh week compared to control (0.83% DW). Addition of 25 μM ASA resulted in 1.44% DW daidzin (1.5-fold increase) with 0.91% DW in control after fifth week and 1.44% DW daidzin (2.3-fold increase) after eighth week when compared to untreated control (0.62% DW). Reduced biomass with increased daidzin content was facilitated by elicited hairy root cultures.
authors.affiliation: a Centre for Plant Molecular Biology (CPMB) , Osmania University , Hyderabad 500007 , Telangana , India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Zaheer
authors.affiliation: a Centre for Plant Molecular Biology (CPMB) , Osmania University , Hyderabad 500007 , Telangana , India.
authors.forename: Vudem Dashavantha
authors.initials: VD
authors.lastname: Reddy
authors.affiliation: a Centre for Plant Molecular Biology (CPMB) , Osmania University , Hyderabad 500007 , Telangana , India.
authors.forename: Charu Chandra
authors.initials: CC
authors.lastname: Giri
mesh_terms: D001241:Aspirin / Q000494:pharmacology*; D018533:Biomass; D003517:Cyclopentanes / Q000494:pharmacology*; D007529:Isoflavones / Q000096:biosynthesis*; D054883:Oxylipins / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism; D029907:Psoralea / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D007529:Isoflavones; D054883:Oxylipins; C013908:daidzin; C011006:jasmonic acid; D001241:Aspirin
keywords: acetyl salicylic acid; daidzin; elicitation; hairy root culture; jasmonic acid; yield enhancement