title: A nanoplasmonic biosensor for label-free multiplex detection of cancer biomarkers.
abstract: The development of highly sensitive, selective and multiplex sensors has become an important challenge for disease diagnosis. In this study, we describe a multiplex biosensor for the detection of cancer biomarkers based on unique plasmon response of single gold nanoparticles (AuNPs) and antibody-antigen binding activity. To demonstrate the ability of the plasmon biosensor to detect and quantify cancer biomarkers: a panel of biomarkers, including α-fetoprotein (AFP), carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) was used as a model analyte for multiple detection. A novel and sensitive multiplex biosensor was developed by immobilizing plasmonic nanoparticles in a site-specific manner and functionalized with monoclonal antibodies that recognize the target protein on hydrophilic-hydrophobic patterned glass slide. The proposed multi-analyte biosensor exhibited outstanding selectivity and sensitivity. The limit of detection was determined to be 91 fM, 94 fM and 10 fM for AFP, CEA and PSA from patient-mimicked serum, respectively. Finally, using this sensing strategy, this platform presents an excellent approach for versatile molecular diagnostics in both research and clinical medical fields.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Jong Uk
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Anh H
authors.initials: AH
authors.lastname: Nguyen
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea; Green School, Korea University, Seoul 136-713, Republic of Korea. Electronic address: simsj@korea.ac.kr.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation; D045424:Complex Mixtures / Q000097:blood; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis*; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D014408:Biomarkers, Tumor; D045424:Complex Mixtures; D006046:Gold
keywords: Cancer biomarkers; Gold nanoparticles; Localized surface plasmon resonance; Multiplex detection