title: Thymoquinone Inhibits IL-1β-Induced Inflammation in Human Osteoarthritis Chondrocytes by Suppressing NF-κB and MAPKs Signaling Pathway.
abstract: Thymoquinone, an active ingredient isolated from Nigella sativa, has been reported to have anti-inflammatory effects. However, the anti-inflammatory effect of thymoquinone on IL-1β-stimulated osteoarthritis chondrocytes remains unclear. In this study, we designed to investigate the anti-inflammatory effects and elucidated the underlying mechanism of thymoquinone on IL-1β-stimulated human osteoarthritis chondrocytes. The effects of thymoquinone on inflammatory mediators COX-2, iNOS, NO, PGE2, as well as MMP-1, MMP3, MMP13 production were detected. The results demonstrated that thymoquinone concentration-dependently inhibited IL-1β-induced COX-2, iNOS, NO, and PGE2 production. Thymoquinone also suppressed IL-1β-induced MMP-1, MMP3, and MMP13 production. We found that thymoquinone significantly inhibited IL-1β-induced NF-κB activation and IκBα degradation. In addition, thymoquinone was found to suppress IL-1β-induced mitogen-activated protein kinases (MAPKs) activation. In conclusion, thymoquinone inhibited IL-1β-induced inflammatory mediator production by inhibition of NF-κB and MAPKs signaling pathways in osteoarthritis chondrocytes. Thymoquinone may be a potential agent in the treatment of osteoarthritis.
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jiutao
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Tianxin
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. guandehonglb@163.com.
authors.forename: Dehong
authors.initials: D
authors.lastname: Guan
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000188:drug therapy* / Q000201:enzymology / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D016227:Benzoquinones; D020672:I-kappa B Proteins; C508585:IL1B protein, human; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha; D020928:Mitogen-Activated Protein Kinases; C003466:thymoquinone
keywords: IL-1β; NF-κB; osteoarthritis chondrocyte; thymoquinone