title: High serum levels of extracellular vesicles expressing malignancy-related markers are released in patients with various types of hematological neoplastic disorders.
abstract: Many cell types release extracellular vesicles (EVs), including exosomes, microvesicles (MVs), and apoptotic bodies, which play a role in physiology and diseases. Presence and phenotype of circulating EVs in hematological malignancies (HMs) remain largely unexplored.The aim of this study was to characterize EVs in peripheral blood of HM patients compared to healthy subjects (controls). We isolated serum EVs from patients with chronic lymphocytic leukemia (CLL), non-Hodgkin's lymphoma (NHL), Waldenstrom's macroglobulinemia (WM), Hodgkin's lymphoma (HL), multiple myeloma (MM), acute myeloid leukemia (AML), myeloproliferative neoplasms (MPNs), myelodysplastic syndromes (MDS), and controls. EVs were isolated from serum of peripheral blood by ultracentrifuge steps and analyzed by flow cytometry to define count, size, and immunophenotype. MV levels were significantly elevated in WM, HL, MM, AML, and some MPNs and, though at a lesser degree, in CLL and NHL as compared to healthy controls. HL, MM, and MPNs generated a population of MVs characterized by lower size (below 0.3 μm) when compared to controls. MVs from patients specifically expressed tumor-related antigens, such as CD19 in B cell neoplasms, CD38 in MM, CD13 in myeloid tumors, and CD30 in HL. Both total and antigen-specific count of MVs significantly correlated with different HM clinical features such as Rai stage in CLL, International Prognostic Scoring System in WM, International Staging System in MM, and clinical stage in HL. MVs may represent a novel biomarker in HMs.
authors.affiliation: Laboratory of Preclinical and Translational Research, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. caivanoa@libero.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Caivano
authors.affiliation: Laboratory of Preclinical and Translational Research, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. ilaria.laurenzana@crob.it.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Laurenzana
authors.affiliation: Laboratory of Preclinical and Translational Research, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. luciana.deluca@crob.it.
authors.forename: Luciana
authors.initials: L
authors.lastname: De Luca
authors.affiliation: Laboratory of Preclinical and Translational Research, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. francesco.larocca@crob.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: La Rocca
authors.affiliation: Laboratory of Preclinical and Translational Research, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. vittorio.simeon@crob.it.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Simeon
authors.affiliation: Laboratory of Preclinical and Translational Research, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. stefania.trino@crob.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Trino
authors.affiliation: Laboratory of Clinical Research and Advanced Diagnostics, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. fiorella.dauria@crob.it.
authors.forename: Fiorella
authors.initials: F
authors.lastname: D'Auria
authors.affiliation: Unit of Clinical Pathology, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. antonio.traficante@crob.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Traficante
authors.affiliation: Unit of Clinical Pathology, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. maddalena.maietti@crob.it.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Maietti
authors.affiliation: Department of Onco-Hematology, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. tiziana.izzo@crob.it.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Izzo
authors.affiliation: Department of Onco-Hematology, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. giovanni.darena@crob.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: D'Arena
authors.affiliation: Department of Onco-Hematology, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. giovanna.mansueto@crob.it.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mansueto
authors.affiliation: Department of Onco-Hematology, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. ematologia45@alice.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pietrantuono
authors.affiliation: Department of Hematology, Catholic University of the Sacred Heart, Rome, Italy. l.laurenti@rm.unicatt.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Laurenti
authors.affiliation: Scientific Direction, IRCCS-CROB, Via Padre Pio, 1, 85128, Rionero in Vulture, Italy. p.musto@crob.it.
authors.forename: Pellegrino
authors.initials: P
authors.lastname: Musto
authors.affiliation: CEINGE-Biotecnologie Avanzate s.c.a.r.l, "Federico II" University, Naples, Italy. luigi.delvecchio@unina.it.|Department of Molecular Medicine and Medical Biotechnology, "Federico II" University, Naples, Italy. luigi.delvecchio@unina.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Vecchio
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D000067128:Extracellular Vesicles / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000097:blood* / Q000145:classification / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor
keywords: Extracellular vesicles; Flow cytometry; Hematological malignancies; Microvesicles