title: Urine survivin, E-cadherin and matrix metalloproteinases as novel biomarkers in children with chronic kidney disease.
abstract: BACKGROUND
  Epithelial-mesenchymal transition (EMT) leads to renal fibrosis and chronic kidney disease (CKD).
  
  
  OBJECTIVE
  The aim of this study was to assess the usefulness of survivin, E-cadherin and metalloproteinases (MMPs) as biomarkers of CKD-related complications.
  
  
  METHODS
  Survivin, E-cadherin, MMP-2, MMP-9 and TGFbeta1 were assessed by ELISA in 41 children with CKD stages 3 to 5 and in 23 controls.
  
  
  RESULTS
  The serum and urine values of analyzed parameters were significantly elevated in CKD patients versus controls and correlated with each other.
  
  
  CONCLUSIONS
  The observed parameter changes indicate apoptosis, tissue remodeling and fibrosis in CKD children. Urine survivin may become a new biomarker of kidney-specific EMT.
authors.affiliation: a Department of Pediatric Nephrology , Wrocław Medical University , Wrocław , Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Musiał
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bargenda
authors.forename: Danuta
authors.initials: D
authors.lastname: Zwolińska
mesh_terms: D000293:Adolescent; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood / Q000652:urine; D015820:Cadherins / Q000097:blood / Q000652:urine*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005355:Fibrosis; D005919:Glomerular Filtration Rate; D006801:Humans; D007223:Infant; D051034:Inhibitor of Apoptosis Proteins / Q000097:blood / Q000652:urine*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000652:urine*; D020780:Matrix Metalloproteinase 9 / Q000097:blood / Q000652:urine*; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000175:diagnosis / Q000473:pathology / Q000652:urine*; D000077022:Survivin; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000652:urine
chemical_list: C107489:BIRC5 protein, human; D015415:Biomarkers; D015820:Cadherins; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D053773:Transforming Growth Factor beta1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Apoptosis; Hsp27; TGFbeta1; epithelial–mesenchymal transition; fibrosis; sFas