title: Effect of the Conjugation Density of Triphenylphosphonium Cation on the Mitochondrial Targeting of Poly(amidoamine) Dendrimers.
abstract: Many clinically relevant diseases with known poor therapeutic outcomes, including cancer and neurodegenerative disorders, have been directly linked to mitochondrial dysfunction. The ability to efficiently target therapeutics to intracellular organelles such as mitochondria may represent new opportunities for the effective treatment of such ailments. The present study reports the synthesis, cellular uptake, cytotoxicity, and mitochondrial colocalization of conjugates of triphenylphosphonium cation (TPP) to amine-terminated, generation 4, poly(amidoamine) (PAMAM) dendrimer (G4NH2) nanocarriers. The mitochondrial-targeting moiety TPP was either directly conjugated to G4NH2 (G4NH2-TPP) or to the dendrimer through a flexible polyethylene glycol (PEG) linker (G4NH2-PEGTPP). Conjugation was done at various TPP densities to assess their biological activity and potential for mitochondrial-targeted drug delivery. Tests in an in vitro model of the human alveolar carcinoma (A549 cells) showed that even at a low TPP density (∼5 TPP) both the cellular internalization and mitochondrial targeting increase significantly, as determined by fluorescence activated cell sorting (FACS) and confocal microscopy (CM), respectively. At a density of ∼10 TPP per G4NH2, further increase in cellular internalization and mitochondrial targeting was achieved. However, at this higher density, the nanocarriers also showed pronounced cytotoxicity. It was observed that the toxicity of the conjugates is decreased upon the addition of a PEG linker between the dendrimer and TPP (G4NH2-PEGTPP), while the mitochondrial targeting ability of the nanocarriers is not affected as the PEG density increases. The proposed strategies indicate that TPP-conjugated G4NH2 dendrimers represent a potentially viable strategy for the targeting of therapeutic molecules to mitochondria, which may help improve therapeutic outcomes of diseases related to mitochondrial dysfunction.
authors.affiliation: Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, Michigan 48202, United States.
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Bielski
authors.affiliation: Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, Michigan 48202, United States.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhong
authors.affiliation: Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, Michigan 48202, United States.
authors.forename: Matthew
authors.initials: M
authors.lastname: Brown
authors.affiliation: Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, Michigan 48202, United States.
authors.forename: Sandro R P
authors.initials: SR
authors.lastname: da Rocha
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D050091:Dendrimers / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008928:Mitochondria / Q000187:drug effects* / Q000473:pathology; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011073:Polyamines / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D009943:Organophosphorus Compounds; C531249:Poly(amidoamine); D011073:Polyamines; D011092:Polyethylene Glycols
keywords: PAMAM dendrimers; PEG; TPP; drug delivery; mitochondrial targeting; polyethylene glycol; triphenylphosphonium cation