title: A multicolor time-resolved fluorescence aptasensor for the simultaneous detection of multiplex Staphylococcus aureus enterotoxins in the milk.
abstract: Food safety is one of the most important public health issues worldwide. Foodborne illnesses caused by Staphylococcus aureus enterotoxins (SEs) commonly occur, affecting both developing and developed countries. In this study, multicolor lanthanide-doped time-resolved fluorescence nanoparticles labeled with aptamers were used as bioprobes, and graphene oxide (GO) was employed as a resonance energy acceptor. Based on the "turn down" strategy, the simultaneous detection of multiplex SEs was realized in a homogeneous solution. Under the optimal conditions, the developed method exhibited high sensitivity and selectivity to three serological types of enterotoxins, including type A, B, C1, with limits of detection below 1 ng mL(-1). The application of this bioassay in milk analysis with no sample dilution was also investigated, and the results of recovery rates covered from 92.76% to 114.58%, revealing that the developed method was accurate. Therefore, this detection aptasnesor can be a good candidate for multiplex analysis and screening with simple and effective operations.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food and Bioengineering, Xihua University, Chengdu 610039, China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: China Rural Technology Development Center, Beijing 100045, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Lab Meat Processing & Quality Control, Yurun Group, Nanjing 210041, China.
authors.forename: Baocai
authors.initials: B
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004768:Enterotoxins / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D052157:Aptamers, Nucleotide; D004768:Enterotoxins; D010087:Oxides; D006108:Graphite
keywords: Aptasensor; Fluorescence resonance energy transfer; Multicolor time-resolved fluorescence; Multiplexed Staphylococcus aureus enterotoxins; Simultaneous detection