title: The composition and functional properties of whey protein concentrates produced from buttermilk are comparable with those of whey protein concentrates produced from skimmed milk.
abstract: The demand for whey protein is increasing in the food industry. Traditionally, whey protein concentrates (WPC) and isolates are produced from cheese whey. At present, microfiltration (MF) enables the utilization of whey from skim milk (SM) through milk protein fractionation. This study demonstrates that buttermilk (BM) can be a potential source for the production of a WPC with a comparable composition and functional properties to a WPC obtained by MF of SM. Through the production of WPC powder and a casein- and phospholipid (PL)-rich fraction by the MF of BM, sweet BM may be used in a more optimal and economical way. Sweet cream BM from industrial churning was skimmed before MF with 0.2-µm ceramic membranes at 55 to 58°C. The fractionations of BM and SM were performed under the same conditions using the same process, and the whey protein fractions from BM and SM were concentrated by ultrafiltration and diafiltration. The ultrafiltration and diafiltration was performed at 50°C using pasteurized tap water and a membrane with a 20-kDa cut-off to retain as little lactose as possible in the final WPC powders. The ultrafiltrates were subsequently spray dried, and their functional properties and chemical compositions were compared. The amounts of whey protein and PL in the WPC powder from BM (BMWPC) were comparable to the amounts found in the WPC from SM (SMWPC); however, the composition of the PL classes differed. The BMWPC contained less total protein, casein, and lactose compared with SMWPC, as well as higher contents of fat and citric acid. No difference in protein solubility was observed at pH values of 4.6 and 7.0, and the overrun was the same for BMWPC and SMWPC; however, the BMWPC made less stable foam than SMWPC.
authors.affiliation: TINE SA R&D, Kalbakken, PO Box 7, Kalbakken, 0901, Oslo, Norway; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, 1432 Ås, Norway. Electronic address: sigrid.svanborg@tine.no.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Svanborg
authors.affiliation: TINE SA R&D, Kalbakken, PO Box 7, Kalbakken, 0901, Oslo, Norway; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, 1432 Ås, Norway.
authors.forename: Anne-Grethe
authors.initials: AG
authors.lastname: Johansen
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, 1432 Ås, Norway.
authors.forename: Roger K
authors.initials: RK
authors.lastname: Abrahamsen
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, 1432 Ås, Norway.
authors.forename: Siv B
authors.initials: SB
authors.lastname: Skeie
mesh_terms: D000818:Animals; D002364:Caseins / Q000032:analysis; D002611:Cheese; D043302:Cultured Milk Products / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis; D012995:Solubility; D013649:Taste; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins; D007785:Lactose
keywords: buttermilk; microfiltration; milk fat globule membrane; phospholipid; whey protein concentrate