title: Susceptibility of northern corn rootworm Diabrotica barberi (Coleoptera: Chrysomelidae) to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins.
abstract: The susceptibility of the northern corn rootworm Diabrotica barberi (Smith & Lawrence) to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis (Bt) was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Larval mortality was evaluated after 7 d. The mCry3A and eCry3.1Ab proteins were found to be toxic to the northern corn rootworm larvae. The LC50and LC99values for mCry3A were 5.13 and 2482.31 μg/mL, respectively. For eCry3.1Ab, the LC50and LC99values were 0.49 and 213.01 μg/mL. Based on the estimated lethal concentrations, eCry3.1Ab protein was more efficacious to northern corn rootworm larvae than mCry3A. These lethal concentration values will be used as diagnostic doses for routine annual monitoring for change in susceptibility of field collected northern corn rootworm to mCry3A, and eCry3.1Ab toxins.
authors.affiliation: Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA.
authors.forename: Isaac O
authors.initials: IO
authors.lastname: Oyediran
authors.affiliation: Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA.
authors.forename: Phillip
authors.initials: P
authors.lastname: Matthews
authors.affiliation: Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA.
authors.forename: Narendra
authors.initials: N
authors.lastname: Palekar
authors.affiliation: USDA ARS, North Central Agricultural Research Laboratory, Brookings, SD, 57006, USA.
authors.forename: Wade
authors.initials: W
authors.lastname: French
authors.affiliation: Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA.
authors.forename: Jared
authors.initials: J
authors.lastname: Conville
authors.affiliation: Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA.
authors.forename: Tony
authors.initials: T
authors.lastname: Burd
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism* / Q000633:toxicity; D001517:Coleoptera; D004731:Endotoxins / Q000378:metabolism* / Q000633:toxicity; D006460:Hemolysin Proteins / Q000378:metabolism* / Q000633:toxicity; D007814:Larva
chemical_list: D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins
keywords: Bacillus thuringiensis; Diabrotica barberi; eCry3.1Ab; mCry3A; susceptibility