title: An"ON-OFF" switchable power output of enzymatic biofuel cell controlled by thermal-sensitive polymer.
abstract: A novel "ON-OFF" switchable enzymatic biofuel cell (EBFC), controlled by in situ thermal-stimuli signal, has been consciously designed. Poly (N-isopropylacrylamide) (PNIPAm) chains were used to act as "ON" and "OFF" channels. Consecutively switching of temperature below and above the lower critical solution temperature (LCST), the reversible conformation changing of the PNIPAm chains between superhydrophilicity and superhydrophobicity was achieved, which constructed the "ON" and "OFF" channel for the transfer of the electrochemical probe to the underlying electrode correspondingly. Gold nanoparticles (AuNPs) protected glucose oxidase and laccase were successfully entrapped into the intelligent thermal-sensitive PNIPAm chains, and performed as the catalysts for the oxidation of glucose and the reduction of O2, respectively. Below the LCST, the fuels and the mediator could access to the catalytic centers of enzymes (set as "ON" state); while above the LCST, the reaction was impeded because the process of reactant transmission was blocked (set as "OFF" state). By switching the "valve" of mass transfer, the fabricated EBFC displayed the obvious "ON-OFF" controllable behavior. At the "ON" state, the open circuit voltage (Ecell(ocv)) and maximal power output density (Pmax) could reach to 0.70 V and 20.52 μW cm(-2), respectively; while at the "OFF" state, the Ecell(ocv) and Pmax were only 0.30 V and 3.28 μW cm(-2) correspondingly. The switchable process was repeatable, and the response time was only several minutes.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Gai
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; School of Chemistry and Life Science, Nanjing University Jinling College, Nanjing 210089, PR China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology*; D001674:Bioelectric Energy Sources; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D013696:Temperature; D055454:Trametes / Q000201:enzymology*
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; C052970:poly-N-isopropylacrylamide; D006046:Gold; D005949:Glucose Oxidase; D042845:Laccase
keywords: Enzymatic biofuel cell; Gold nanoparticles; Lower critical solution temperature; Poly (N-isopropylacrylamide); “ON–OFF” switchable power output