title: Modulation of superoxide dismutase (SOD) isozymes by organ development and high long-term salinity in the halophyte Cakile maritima.
abstract: Superoxide dismutase (SOD) activity catalyzes the disproportionation of superoxide radicals into hydrogen peroxide and oxygen. This enzyme is considered to be a first line of defense for controlling the production of reactive oxygen species (ROS). In this study, the number and type of SOD isozymes were identified in the principal organs (roots, stems, leaves, flowers, and seeds) of Cakile maritima. We also analyzed the way in which the activity of these SOD isozymes is modulated during development and under high long-term salinity (400 mM NaCl) stress conditions. The data indicate that this plant contains a total of ten SOD isozymes: two Mn-SODs, one Fe-SOD, and seven CuZn-SODs, with the Fe-SOD being the most prominent isozyme in the different organs analyzed. Moreover, the modulation of SOD isozymes, particularly CuZn-SODs, was only detected during development and under severe salinity stress conditions. These data suggest that, in C. maritima, the occurrence of these CuZn-SODs in roots and leaves plays an adaptive role since this CuZn-SOD isozyme might replace the diminished Fe-SOD activity under salinity stress to overcome this adverse environmental condition.
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080, Granada, Spain.|Laboratory of Extremophile Plants, Center of Biotechnology of Borj Cedria, PO Box 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Hayet
authors.initials: H
authors.lastname: Houmani
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080, Granada, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Rodríguez-Ruiz
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080, Granada, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Palma
authors.affiliation: Laboratory of Extremophile Plants, Center of Biotechnology of Borj Cedria, PO Box 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.affiliation: Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080, Granada, Spain. javier.corpas@eez.csic.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Corpas
mesh_terms: D019607:Brassicaceae / Q000201:enzymology* / Q000254:growth & development*; D035264:Flowers / Q000201:enzymology / Q000254:growth & development; D007527:Isoenzymes / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000254:growth & development; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology / Q000254:growth & development; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000201:enzymology / Q000254:growth & development; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D013482:Superoxide Dismutase
keywords: Cakile maritima; Fe-SOD; Halophyte; SOD isozymes; Salt stress