title: miR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factor.
abstract: This study aims to investigate the regulative effects of microRNA-451a (miR-451a) on cell proliferation and sensitivity to tamoxifen in breast cancer cells. In cell culture experiments, the lentiviral vectors of pHBLV-miR-451a and pHBLV-miR-451a sponge were constructed and used to transfect MCF-7 and LCC2 cells. The transfection efficiency was tested by fluorescent observation, and cell lines with stable over- or downregulated expression of miR-451a were established. The expression of miR-451a and the target gene macrophage migration inhibitory factor (MIF) were detected by real-time reverse transcriptase polymerase chain reaction and/or western blot. Moreover, MTT assay, colony formation, and Transwell invasion assays were also performed. Data showed that the recombinant lentiviral vectors were constructed correctly, and the virus titer was 1 × 10(8) CFU/mL. The stable transfected cells were obtained. Overexpression of miR-451a downregulated MIF expression in mRNA and protein levels and inhibited cell proliferation, colony formation, and invasion of breast cancer cells. Downregulation of miR-451a upregulated MIF expression and increased breast cancer cell growth, invasion, and tamoxifen sensitivity. In summary, the miR-451a/MIF pathway may play important roles in the biological properties of breast cancer cells and may be a potential therapeutic target for breast cancer.
authors.affiliation: Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China ; Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Zhenru
authors.initials: Z
authors.lastname: Liu
authors.affiliation: West China School of Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Miao
authors.affiliation: Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
authors.affiliation: West China School of Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Zhouhua
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: West China School of Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China ; 985 Science and Technology Platform for Innovative Drugs of Sichuan University, Chengdu 610041, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000378:metabolism; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013629:Tamoxifen / Q000494:pharmacology / Q000627:therapeutic use*; D014161:Transduction, Genetic; D014162:Transfection; D019065:Virus Assembly / Q000187:drug effects
chemical_list: C531201:MIRN451 microRNA, human; D008263:Macrophage Migration-Inhibitory Factors; D035683:MicroRNAs; D013629:Tamoxifen; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human