title: Enzymatic Saccharification of Lignocellulosic Residues by Cellulases Obtained from Solid State Fermentation Using Trichoderma viride.
abstract: The aim of this study was to verify the viability of lignocellulosic substrates to obtain renewable energy source, through characterization of the cellulolytic complex, which was obtained by solid state fermentation using Trichoderma viride. Enzymatic activity of the cellulosic complex was measured during saccharification of substrates filter paper, eucalyptus sawdust, and corncob, and compared with the activity of commercial cellulase. The characterization of the enzymes was performed by a 2(2) Full Factorial Design, where the pH and temperature were the variables of study. Enzymatic saccharification of different substrates appearedviable until 12 to be viable until 12 h; after this period the activity decreased for both enzymatic forms (cellulolytic complex and commercial cellulase). The enzymatic activity of the commercial cellulase was favored with the use of corncob as substrate, while the cellulolytic complex does not show any difference in its specificity by the substrates studied. The largest activities of both enzymes were obtained in the temperature and pH range between 40°C and 50°C and 4.8 and 5.2, respectively. The cellulolytic complex obtained appeared to be viable for the saccharification of lignocellulosic residues compared with the commercial cellulase.
authors.affiliation: Laboratory of Fermentations, Course of Food Engineering, School of Engineering and Architecture, University of Passo Fundo, Campus I, 99052900 Passo Fundo, RS, Brazil ; Department of Food Engineering, School of Food Engineering, University of Campinas, Campinas, SP, Brazil.
authors.forename: Tanara
authors.initials: T
authors.lastname: Sartori
authors.affiliation: Laboratory of Fermentations, Course of Food Engineering, School of Engineering and Architecture, University of Passo Fundo, Campus I, 99052900 Passo Fundo, RS, Brazil ; Department of Food Engineering, School of Food Engineering, University of Campinas, Campinas, SP, Brazil.
authors.forename: Heloisa
authors.initials: H
authors.lastname: Tibolla
authors.affiliation: Laboratory of Fermentations, Course of Food Engineering, School of Engineering and Architecture, University of Passo Fundo, Campus I, 99052900 Passo Fundo, RS, Brazil.
authors.forename: Elenizi
authors.initials: E
authors.lastname: Prigol
authors.affiliation: Laboratory of Fermentations, Course of Food Engineering, School of Engineering and Architecture, University of Passo Fundo, Campus I, 99052900 Passo Fundo, RS, Brazil.
authors.forename: Luciane Maria
authors.initials: LM
authors.lastname: Colla
authors.affiliation: Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University Foundation of Rio Grande, Rio Grande, RS, Brazil.
authors.forename: Jorge Alberto Vieira
authors.initials: JA
authors.lastname: Costa
authors.affiliation: Laboratory of Fermentations, Course of Food Engineering, School of Engineering and Architecture, University of Passo Fundo, Campus I, 99052900 Passo Fundo, RS, Brazil.
authors.forename: Telma Elita
authors.initials: TE
authors.lastname: Bertolin
mesh_terms: D044602:Cellulases / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D059205:Renewable Energy; D013696:Temperature; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D008031:Lignin; D044602:Cellulases