title: Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization.
abstract: The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-β-M and F4H5-β-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-β-maltoside (DD-β-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar interactions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.
authors.affiliation: Institut des Biomolécules Max Mousseron/CBSA, UMR 5247, Avignon University, 33 Rue Louis Pasteur, 84000 Avignon,France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Dahani
authors.affiliation: Institut des Biomolécules Max Mousseron/CBSA, UMR 5247, Avignon University, 33 Rue Louis Pasteur, 84000 Avignon,France.
authors.forename: Laurie Anne
authors.initials: LA
authors.lastname: Barret
authors.affiliation: Institut des Biomolécules Max Mousseron/CBSA, UMR 5247, Avignon University, 33 Rue Louis Pasteur, 84000 Avignon,France.
authors.forename: Simon
authors.initials: S
authors.lastname: Raynal
authors.affiliation: Laboratoire de Bioénergétique Cellulaire/Biologie Végétale et Microbiologie Environnementales, UMR 7265, 13108 Saint-Paul-lez-Durance, France.
authors.forename: Colette
authors.initials: C
authors.lastname: Jungas
authors.affiliation: European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Pétra
authors.initials: P
authors.lastname: Pernot
authors.affiliation: Institut des Biomolécules Max Mousseron/CBSA, UMR 5247, Avignon University, 33 Rue Louis Pasteur, 84000 Avignon,France.
authors.forename: Ange
authors.initials: A
authors.lastname: Polidori
authors.affiliation: Institut des Biomolécules Max Mousseron/CBSA, UMR 5247, Avignon University, 33 Rue Louis Pasteur, 84000 Avignon,France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Bonneté
mesh_terms: D003460:Crystallization; D000067493:Dynamic Light Scattering / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry*; D053838:Scattering, Small Angle; D012996:Solutions; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D008565:Membrane Proteins; D012996:Solutions; D013501:Surface-Active Agents
keywords: crystallization; membrane protein; phase diagram; scattering techniques; surfactant micelle