title: Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach.
abstract: A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8%) and accuracy (recoveries ≥96.7%). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
authors.affiliation: REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal.
authors.forename: Rita C
authors.initials: RC
authors.lastname: Alves
authors.forename: Filipa B
authors.initials: FB
authors.lastname: Pimentel
authors.forename: Henri P A
authors.initials: HP
authors.lastname: Nouws
authors.forename: Wagner
authors.initials: W
authors.lastname: Correr
authors.forename: María Begoña
authors.initials: MB
authors.lastname: González-García
authors.forename: M Beatriz P P
authors.initials: MB
authors.lastname: Oliveira
authors.forename: Cristina
authors.initials: C
authors.lastname: Delerue-Matos
mesh_terms: D055516:2S Albumins, Plant / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D059022:Food Safety / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D055516:2S Albumins, Plant; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C492737:Ara h 6 allergen, Arachis hypogaea