title: Report: Bioconversion of agriculture waste to lysine with UV mutated strain of brevibacterium flavum and its biological evaluation in broiler chicks.
abstract: Lysine executes imperative structural and functional roles in body and its supplementation in diet beneficial to prevent the escalating threat of protein deficiency. The physical mutagenesis offers new fascinating avenues of research for overproduction of lysine through surplus carbohydrate containing agriculture waste especially in developing countries. The current study was aimed to investigate the potential of UV mutated strain of Brevibacterium flavum at 254 nm for lysine production. The physical and nutritional parameters were optimized and maximum lysine production was observed with molasses (4% substrate water ratio). Moreover, supplementation of culture medium with metal cations (i.e. 0.4% CaSO₄, 0.3% NaCl, 0.3% KH₂PO₄, 0.4% MgSO₄, and 0.2% (NH₄) ₂SO₄w/v) together with 0.75% v/v corn steep liquor significantly enhanced the lysine production up to 26.71 ± 0.31 g/L. Though, concentrations of urea, ammonium nitrate and yeast sludge did not exhibit any profound effect on lysine production. Biological evaluation of lysine enriched biomass in terms of weight gain and feed conversion ratio reflected non-significant difference for experimental and control (+ve) groups. Conclusively, lysine produced in the form of biomass was compatible to market lysine in its effectiveness and have potential to utilize at commercial scale.
authors.affiliation: Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Alia
authors.initials: A
authors.lastname: Tabassum
authors.affiliation: Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Abu Saeed
authors.initials: AS
authors.lastname: Hashmi
authors.affiliation: Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Faiza
authors.initials: F
authors.lastname: Masood
authors.affiliation: Department of Food Science and Human Nutrition, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Muhammad Aamir
authors.initials: MA
authors.lastname: Iqbal
authors.affiliation: Department of Food Science and Human Nutrition, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Tayyab
authors.affiliation: Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Amber
authors.initials: A
authors.lastname: Nawab
authors.affiliation: Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
authors.forename: Asif
authors.initials: A
authors.lastname: Nadeem
authors.affiliation: Department of Production and Utilization of Medicinal Plants, Faculty of Agricultural and Natural Resources, Higher Educational Complex of Saravan, Saravan, PO Box 9951634145, Sistan and Baluchistan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Sadeghi
authors.affiliation: Environmental Biology and Ecotoxicology Laboratory, Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan.
authors.forename: Adeel
authors.initials: A
authors.lastname: Mahmood
mesh_terms: D000383:Agriculture; D000818:Animals; D018533:Biomass; D040142:Brevibacterium flavum / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D002645:Chickens / Q000254:growth & development*; D008239:Lysine / Q000096:biosynthesis*; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D012965:Sodium Chloride; D008239:Lysine; D009584:Nitrogen