title: Defining Mononuclear Phagocyte Subset Homology Across Several Distant Warm-Blooded Vertebrates Through Comparative Transcriptomics.
abstract: Mononuclear phagocytes are organized in a complex system of ontogenetically and functionally distinct subsets, that has been best described in mouse and to some extent in human. Identification of homologous mononuclear phagocyte subsets in other vertebrate species of biomedical, economic, and environmental interest is needed to improve our knowledge in physiologic and physio-pathologic processes, and to design intervention strategies against a variety of diseases, including zoonotic infections. We developed a streamlined approach combining refined cell sorting and integrated comparative transcriptomics analyses which revealed conservation of the mononuclear phagocyte organization across human, mouse, sheep, pigs and, in some respect, chicken. This strategy should help democratizing the use of omics analyses for the identification and study of cell types across tissues and species. Moreover, we identified conserved gene signatures that enable robust identification and universal definition of these cell types. We identified new evolutionarily conserved gene candidates and gene interaction networks for the molecular regulation of the development or functions of these cell types, as well as conserved surface candidates for refined subset phenotyping throughout species. A phylogenetic analysis revealed that orthologous genes of the conserved signatures exist in teleost fishes and apparently not in Lamprey.
authors.affiliation: UM2, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université , Marseille , France ; U1104, INSERM , Marseille , France ; UMR7280, CNRS , Marseille , France.
authors.forename: Thien-Phong
authors.initials: TP
authors.lastname: Vu Manh
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Jamila
authors.initials: J
authors.lastname: Elhmouzi-Younes
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Céline
authors.initials: C
authors.lastname: Urien
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Suzana
authors.initials: S
authors.lastname: Ruscanu
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Luc
authors.initials: L
authors.lastname: Jouneau
authors.affiliation: IFR87 La Plante et son Environnement, IMAGIF CNRS , Gif-sur-Yvette , France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Bourge
authors.affiliation: CRB GADIE, Génétique Animale et Biologie Intégrative, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Marco
authors.initials: M
authors.lastname: Moroldo
authors.affiliation: UMR1225, Université de Toulouse, INPT, ENVT , Toulouse , France ; UMR1225, Interactions Hôtes-Agents Pathogènes, INRA , Toulouse , France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Foucras
authors.affiliation: UMR1282, Infectiologie et Santé Publique, INRA , Nouzilly , France ; UMR1282, Université François Rabelais de Tours , Tours , France.
authors.forename: Henri
authors.initials: H
authors.lastname: Salmon
authors.affiliation: UMR1282, Infectiologie et Santé Publique, INRA , Nouzilly , France ; UMR1282, Université François Rabelais de Tours , Tours , France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Marty
authors.affiliation: UMR1282, Infectiologie et Santé Publique, INRA , Nouzilly , France ; UMR1282, Université François Rabelais de Tours , Tours , France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Quéré
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertho
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudinot
authors.affiliation: UM2, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université , Marseille , France ; U1104, INSERM , Marseille , France ; UMR7280, CNRS , Marseille , France.
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
authors.affiliation: UR892, Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert , Jouy-en-Josas , France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
keywords: comparative biology; dendritic cells; genomic and bio-informatic methods; immunology; macrophages; monocytes