title: Cold Microwave-Enabled Protein Detection and Quantification.
abstract: Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio is highly beneficial in many research areas. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, Western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 24-45 min.
authors.affiliation: Gastrointestinal Laboratory, Department Small Animal Clinic, Texas A&M University, College Station, TX, 77843, USA.
authors.forename: Niels
authors.initials: N
authors.lastname: Grützner
authors.forename: Romy M
authors.initials: RM
authors.lastname: Heilmann
authors.forename: Aaron G
authors.initials: AG
authors.lastname: Smith
authors.forename: Carol B
authors.initials: CB
authors.lastname: Johnson
authors.forename: Stanislav
authors.initials: S
authors.lastname: Vitha
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
authors.forename: Andreas K
authors.initials: AK
authors.lastname: Holzenburg
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods*; D008872:Microwaves; D011506:Proteins / Q000032:analysis*; D059629:Signal-To-Noise Ratio
chemical_list: D011506:Proteins