title: Transplanted Neural Stem Cells: Playing a Neuroprotective Role by Ceruloplasmin in the Substantia Nigra of PD Model Rats?
abstract: Although mounting evidence suggests that ceruloplasmin (CP) deficiency and iron deposition are pivotal factors responsible for exacerbating demise of dopaminergic neurons in the substantia nigra (SN) of the Parkinsonism and neural stem cells (NSCs) are believed to be excellent candidates for compensating the lost dopaminergic neurons, there are few researches to explore the change of CP expression and of iron deposition in the pathological microenvironment of SN after NSCs transplantation and the ability of grafted NSCs to differentiate directionally into dopaminergic neurons under the changed homeostasis. With substantia nigral stereotaxic technique and NSCs transplantation, we found that tyrosine hydroxylase and CP expression decreased and iron deposition increased in the lesioned SN after 6-OHDA administration compared with control, while tyrosine hydroxylase and CP expression increased and iron deposition decreased after NSCs transplantation compared to 6-OHDA administration alone. Only a small number of embedding NSCs are able to differentiate into dopaminergic neurons. These results suggest that grafted NSCs have an influence on improving the content of CP expression, which may play a neuroprotective role by decreasing iron deposition and ameliorating damage of dopaminergic neurons and possibly underline the iron-related common mechanism of Parkinson's disease and Wilson's disease.
authors.affiliation: Department of Neurology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Xiao
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yin
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ze-Jian
authors.initials: ZJ
authors.lastname: Wang
authors.affiliation: Department of Neurology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wang
mesh_terms: D018663:Adrenergic Agents / Q000008:administration & dosage; D000818:Animals; D001921:Brain / Q000473:pathology; D002570:Ceruloplasmin / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000637:transplantation*; D016627:Oxidopamine / Q000008:administration & dosage; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013378:Substantia Nigra / Q000378:metabolism*; D014184:Transplantation, Homologous; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D018663:Adrenergic Agents; D012333:RNA, Messenger; D016627:Oxidopamine; D007501:Iron; D014446:Tyrosine 3-Monooxygenase; D002570:Ceruloplasmin