title: Octadecaneuropeptide ODN prevents hydrogen peroxide-induced oxidative damage of biomolecules in cultured rat astrocytes.
abstract: Oxidative stress, associated with a variety of disorders including neurodegenerative diseases, is a major cause of cellular dysfunction and biomolecule damages which play a crucial role in neuronal apoptosis. Astrocytes specifically synthesize and release endozepines, a family of regulatory peptides, including the octadecaneuropeptide ODN. We have recently shown that ODN is a potent glioprotective agent that prevents hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis. The purpose of the present study was to investigate the potential protective effect of ODN on oxidative-generated damage of biomolecules in cultured rat astrocytes. Incubation of cells with subnanomolar concentrations of ODN (0.1fM-0.1nM) inhibited H2O2-evoked reactive oxygen species accumulation and cell death in a concentration-dependent manner. Exposure of H2O2-treated cells to 0.1nM ODN inhibited superoxide anion generation and blocked oxidative damage of cell molecules caused by H2O2i.e. formation and accumulation of lipid oxidation products, malondialdehydes and conjugated dienes, and protein carbonyl compounds. Taken together, these data demonstrate for the first time that ODN prevents oxidative stress-induced alteration of cellular constituents. ODN is thus a potential candidate to reduce neuronal damage in various pathological conditions involving oxidative neurodegeneration.
authors.affiliation: Laboratory of Functional Neurophysiology and Pathology, Research Unit UR/11ES09, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, 2092 Tunis, Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Hamdi
authors.affiliation: Laboratory of Functional Neurophysiology and Pathology, Research Unit UR/11ES09, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, 2092 Tunis, Tunisia; CIRB, CNRS UMR 7241, INSERM U1050, College de France, 11 place Marcelin Berthelot, 75231 Paris Cedex 05, France; Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris City, 75205 Paris Cedex, France.
authors.forename: Hadhemi
authors.initials: H
authors.lastname: Kaddour
authors.affiliation: Inserm U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, 76128, Mont-Saint-Aignan, France; Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), Institute for Biomedical Research and Innovation, University of Rouen, Mont-Saint-Aignan, France.
authors.forename: David
authors.initials: D
authors.lastname: Vaudry
authors.affiliation: Inserm U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, 76128, Mont-Saint-Aignan, France; Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), Institute for Biomedical Research and Innovation, University of Rouen, Mont-Saint-Aignan, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.affiliation: UR12ES05, Laboratory of Nutrition-Functional Food & Vascular Health, Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia.
authors.forename: Amira
authors.initials: A
authors.lastname: Zarrouk
authors.affiliation: UR12ES05, Laboratory of Nutrition-Functional Food & Vascular Health, Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
authors.affiliation: Inserm U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, 76128, Mont-Saint-Aignan, France; Regional Platform for Cell Imaging of Haute-Normandie (PRIMACEN), Institute for Biomedical Research and Innovation, University of Rouen, Mont-Saint-Aignan, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.affiliation: Inserm U982, Laboratory of Neuronal and Neuroendocrine Communication and Differentiation, University of Rouen, 76128, Mont-Saint-Aignan, France.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Tonon
authors.affiliation: Laboratory of Functional Neurophysiology and Pathology, Research Unit UR/11ES09, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, 2092 Tunis, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Amri
authors.affiliation: Laboratory of Functional Neurophysiology and Pathology, Research Unit UR/11ES09, Department of Biological Sciences, Faculty of Science of Tunis, University Tunis El Manar, 2092 Tunis, Tunisia. Electronic address: olfa.masmoudi@fst.rnu.tn.
authors.forename: Olfa
authors.initials: O
authors.lastname: Masmoudi-Kouki
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D026261:Diazepam Binding Inhibitor / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009479:Neuropeptides / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats
chemical_list: D026261:Diazepam Binding Inhibitor; D009479:Neuropeptides; D010446:Peptide Fragments; C067529:diazepam binding inhibitor (33-50); D006861:Hydrogen Peroxide
keywords: Astrocytes; Cell death; Lipid peroxidation; Octadecaneuropeptide; Oxidative stress; Protein carbonylation