title: Ipl1/Aurora-B is necessary for kinetochore restructuring in meiosis I in Saccharomyces cerevisiae.
abstract: In mitosis, the centromeres of sister chromosomes are pulled toward opposite poles of the spindle. In meiosis I, the opposite is true: the sister centromeres move together to the same pole, and the homologous chromosomes are pulled apart. This change in segregation patterns demands that between the final mitosis preceding meiosis and the first meiotic division, the kinetochores must be restructured. In budding yeast, unlike mammals, kinetochores are largely stable throughout the mitotic cycle. In contrast, previous work with budding and fission yeast showed that some outer kinetochore proteins are lost in early meiosis. We use quantitative mass spectrometry methods and imaging approaches to explore the kinetochore restructuring process that occurs in meiosis I in budding yeast. The Ndc80 outer kinetochore complex, but not other subcomplexes, is shed upon meiotic entry. This shedding is regulated by the conserved protein kinase Ipl1/Aurora-B and promotes the subsequent assembly of a kinetochore that will confer meiosis-specific segregation patterns on the chromosome.
authors.affiliation: Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.
authors.forename: Régis E
authors.initials: RE
authors.lastname: Meyer
authors.affiliation: Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.
authors.forename: Hoa H
authors.initials: HH
authors.lastname: Chuong
authors.affiliation: Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.
authors.forename: Marrett
authors.initials: M
authors.lastname: Hild
authors.affiliation: Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.
authors.forename: Christina L
authors.initials: CL
authors.lastname: Hansen
authors.affiliation: Program in Free Radical Biology and Aging, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.
authors.forename: Michael
authors.initials: M
authors.lastname: Kinter
authors.affiliation: Program in Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104 Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104 dawsond@omrf.org.
authors.forename: Dean S
authors.initials: DS
authors.lastname: Dawson
mesh_terms: D064127:Aurora Kinases / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D059447:Cell Cycle Checkpoints / Q000235:genetics; D002503:Centromere / Q000378:metabolism; D020090:Chromosome Segregation; D018386:Kinetochores / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D008540:Meiosis / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism
chemical_list: C112472:NDC80 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D064127:Aurora Kinases; C576961:IPL1 protein, S cerevisiae