title: Extracellular protein analysis of activated sludge and their functions in wastewater treatment plant by shotgun proteomics.
abstract: In this work, proteins in extracellular polymeric substances extracted from anaerobic, anoxic and aerobic sludges of wastewater treatment plant (WWTP) were analyzed to probe their origins and functions. Extracellular proteins in WWTP sludges were identified using shotgun proteomics, and 130, 108 and 114 proteins in anaerobic, anoxic and aerobic samples were classified, respectively. Most proteins originated from cell and cell part, and their most major molecular functions were catalytic activity and binding activity. The results exhibited that the main roles of extracellular proteins in activated sludges were multivalence cations and organic molecules binding, as well as in catalysis and degradation. The catalytic activity proteins were more widespread in anaerobic sludge compared with those in anoxic and aerobic sludges. The structure difference between anaerobic and aerobic sludges could be associated with their catalytic activities proteins. The results also put forward a relation between the macro characteristics of activated sludges and micro functions of extracellular proteins in biological wastewater treatment process.
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: 1] Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China [2] Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Guo
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1] Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China [2] Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: You-Peng
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yan
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Ji-Xiang
authors.initials: JX
authors.lastname: Yang
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D007526:Isoelectric Point; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry; D014865:Waste Disposal, Fluid
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D012722:Sewage