title: A Trypsin Inhibitor from Clitoria fairchildiana Cotyledons is Active Against Digestive Enzymes of Aedes aegypti Larvae.
abstract: Aedes aegypti, the principal mosquito vector of yellow fever, dengue fever and chikungunya fever virus-transmitted diseases, is an insect closely associated with humans and their housing habitats. As there is no commercially available vaccine, prevention is the most suggested form of avoiding disease spreading and a number of studies are being developed in order to give support to vector control operations. The present study reports on the identification of a trypsin inhibitor isolated from cotyledons of the Clitoria fairchildiana amazonic tree seeds, which was able to reduce by 87.93 % the activity of digestive enzymes of fourth instar A. aegypti larva. A partial amino acid sequence showed strong similarity with sequences from several trypsin inhibitors already reported in the literature. The 13,000 Da isolated inhibitor was seen to be active solely against trypsin-like enzymes, neither acting on papain, α-amylase nor on other serine proteases, such as elastase, chymotrypsin or subtilisin. At least six from seven active digestive proteases from A. aegypti larvae, visualized by zymography, were severely affected soon after exposed to the inhibitor. The strong and specific action of the isolated inhibitor against trypsin digestive enzymes of this insect vector led us to believe that this protein may be a good candidate for a prospective alternative biocontrol method.
authors.forename: Lucilene O
authors.initials: LO
authors.lastname: de Oliveira
authors.forename: Kátia V S
authors.initials: KV
authors.lastname: Fernandes
authors.forename: Dayanni de Souza
authors.initials: Dde S
authors.lastname: Pádua
authors.forename: André de O
authors.initials: Ade O
authors.lastname: Carvalho
authors.forename: Francisco J A
authors.initials: FJ
authors.lastname: Lemos
authors.forename: Valdirene M
authors.initials: VM
authors.lastname: Gomes
authors.forename: Antônia E A
authors.initials: AE
authors.lastname: Oliveira
authors.forename: André T da Silva
authors.initials: AT
authors.lastname: Ferreira
authors.affiliation: Laboratorio de Quimica e Funcao de Proteinas e Peptideos, CBB, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Avenida Alberto Lamego, 2000 - Parque California, CEP: 28013-602, Campos dos Goytacazes, RJ, Brasil. cowpkat@uenf.br.
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
mesh_terms: D000330:Aedes / Q000201:enzymology*; D000818:Animals; D031292:Clitoria / Q000737:chemistry*; D018548:Cotyledon / Q000737:chemistry*; D006801:Humans; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D007814:Larva / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism*; D010572:Pest Control, Biological; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D019476:Insect Proteins; D014361:Trypsin Inhibitors; D010447:Peptide Hydrolases