title: Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide.
abstract: The application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction between antihypertensive drug captopril and human serum albumin in the absence and presence of diuretic drug hydrochlorothiazide was presented in this work. At near-physiological conditions (67mM phosphate buffer, pH 7.4, I=0.17, 37°C), the individual solution of 100μM captopril and the co-binding solution with 60μM hydrochlorothiazide added were pre-equilibrated with series concentrations of HSA (10-475μM) respectively, introducing hydrodynamically into an uncoated fused silica capillary (35cm×50μm I.D. with 26.5cm effective length). The values of number of binding sites, the binding constant for captopril and hydrochlorothiazide binding to HSA were obtained, respectively. It can be found that both drugs exhibit moderate binding properties towards HSA and there does not exist significant competitive binding effects between them.
authors.affiliation: Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Rd. Zuchongzhi, Zhangjiang Hi-Tech Park, Shanghai 201203, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Liu
authors.affiliation: Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Rd. Zuchongzhi, Zhangjiang Hi-Tech Park, Shanghai 201203, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xiang
authors.affiliation: Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Rd. Zuchongzhi, Zhangjiang Hi-Tech Park, Shanghai 201203, China.
authors.forename: Jian-Ling
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Rd. Zuchongzhi, Zhangjiang Hi-Tech Park, Shanghai 201203, China. Electronic address: dychen00@hotmail.com.
authors.forename: Dong-Ying
authors.initials: DY
authors.lastname: Chen
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry*; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000737:chemistry*; D001667:Binding, Competitive; D002216:Captopril / Q000008:administration & dosage / Q000737:chemistry*; D004338:Drug Combinations; D019075:Electrophoresis, Capillary; D006801:Humans; D006852:Hydrochlorothiazide / Q000008:administration & dosage / Q000737:chemistry*; D008956:Models, Chemical; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D004338:Drug Combinations; D012709:Serum Albumin; D006852:Hydrochlorothiazide; D002216:Captopril
keywords: Binding interaction; Capillary electrophoresis-frontal analysis; Captopril; Human serum albumin; Hydrochlorothiazide