title: Ikaros could be a key factor in the maintenance of "B-side" of B-1 cells.
abstract: Ikaros, a zinc finger transcription factor, is an important regulator of the hematopoietic system. Several studies have suggested the role of Ikaros in the development, maturation, activation and differentiation of lymphocytes. To elucidate this mechanism, it is important to understand how this transcription factor works in the dichotomy of the hematopoietic system, a topic that remains uncertain. Herein, we investigated the role of Ikaros in the control of the lymphomyeloid phenotype of B-1 lymphocytes. We found that Ikaros, as well as its target genes, are expressed in B-1 cells,. Moreover, Ikaros positively regulates the expression of Flt3, Gfi and Il7r, while it down-regulates PU.1. During the induction of differentiation of B-1 cells toward phagocytes, Ikaros transcription was reduced. Taken together, these data pointed to the relevance of Ikaros in the maintenance of the promiscuous gene profile of B-1 cells. It could be suggested that Ikaros functions as a guardian of B-1 lymphoid pattern, and that its absence directs the differentiation of B-1 cells into phagocytes.
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil. Electronic address: vi_bi22@hotmail.com.
authors.forename: Vivian Cristina
authors.initials: VC
authors.lastname: Oliveira
authors.affiliation: Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil. Electronic address: nilbio85@gmail.com.
authors.forename: Nilmar Silvo
authors.initials: NS
authors.lastname: Moretti
authors.affiliation: Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil. Electronic address: leonardo05augusto@gmail.com.
authors.forename: Leonardo da Silva
authors.initials: Lda S
authors.lastname: Augusto
authors.affiliation: Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil. Electronic address: sschenkman@unifesp.br.
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil; Programa de Pós-Graduação em Patologia Ambiental e Experimental-Instituto de Ciências da Saúde Universidade Paulista (UNIP), Brazil. Electronic address: mmariano@unifesp.br.
authors.forename: Mario
authors.initials: M
authors.lastname: Mariano
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil. Electronic address: afpopi@unifesp.br.
authors.forename: Ana Flavia
authors.initials: AF
authors.lastname: Popi
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D051740:Ikaros Transcription Factor / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D010586:Phagocytes / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D051740:Ikaros Transcription Factor; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1
keywords: B-1 cells; Ikaros; PU.1