title: Metabolomics and Natural-Products Strategies to Study Chemical Ecology in Nematodes.
abstract: This review provides an overview of two complementary approaches to identify biologically active compounds for studies in chemical ecology. The first is activity-guided fractionation and the second is metabolomics, particularly focusing on a new liquid chromatography-mass spectrometry-based method called isotopic ratio outlier analysis. To illustrate examples using these approaches, we review recent experiments using Caenorhabditis elegans and related free-living nematodes.
authors.affiliation: *Department of Biochemistry and Molecular Biology and Southeast Center for Integrated Metabolomics, University of Florida, Gainesville, FL 32610-0245, USA; aedison@ufl.edu.
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Edison
authors.affiliation: *Department of Biochemistry and Molecular Biology and Southeast Center for Integrated Metabolomics, University of Florida, Gainesville, FL 32610-0245, USA;
authors.forename: Chaevien S
authors.initials: CS
authors.lastname: Clendinen
authors.affiliation: *Department of Biochemistry and Molecular Biology and Southeast Center for Integrated Metabolomics, University of Florida, Gainesville, FL 32610-0245, USA;
authors.forename: Ramadan
authors.initials: R
authors.lastname: Ajredini
authors.affiliation: *Department of Biochemistry and Molecular Biology and Southeast Center for Integrated Metabolomics, University of Florida, Gainesville, FL 32610-0245, USA; IROA Technologies, Ann Arbor, MI, USA;
authors.forename: Chris
authors.initials: C
authors.lastname: Beecher
authors.affiliation: *Department of Biochemistry and Molecular Biology and Southeast Center for Integrated Metabolomics, University of Florida, Gainesville, FL 32610-0245, USA;
authors.forename: Francesca V
authors.initials: FV
authors.lastname: Ponce
authors.affiliation: The Scripps Research Institute, Department of Molecular and Experimental Medicine, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Stupp
mesh_terms: D000818:Animals; D001688:Biological Products / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000502:physiology; D005591:Chemical Fractionation / Q000379:methods*; D002633:Chemotaxis; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D009348:Nematoda / Q000502:physiology*
chemical_list: D001688:Biological Products