title: Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies.
abstract: With the introduction of next-generation sequencing (NGS) technologies in 2005, the domination of microarrays in genomics quickly came to an end due to NGS's superior technical performance and cost advantages. By enabling genetic analysis capabilities that were not possible previously, NGS technologies have started to play an integral role in all areas of biomedical research. This chapter outlines the low-quantity DNA and cDNA sequencing capabilities and applications developed with the Helicos single molecule DNA sequencing technology.
authors.affiliation: Helicos BioSciences Corporation Cambridge, MA, USA.
authors.forename: Fatih
authors.initials: F
authors.lastname: Ozsolak
mesh_terms: D015894:Genome, Human; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*