title: The Quinovic Acid Glycosides Purified Fraction from Uncaria tomentosa Protects against Hemorrhagic Cystitis Induced by Cyclophosphamide in Mice.
abstract: Uncaria tomentosa is widely used in folk medicine for the treatment of numerous diseases, such as urinary tract disease. Hemorrhagic cystitis (HE) is an inflammatory condition of the bladder associated with the use of anticancer drugs such as cyclophosphamide (CYP). Sodium 2-mercaptoethanesulfonate (Mesna) has been used to prevent the occurrence of HE, although this compound is not effective in established lesions. It has been demonstrated that the purinergic system is involved in several pathophysiological events. Among purinergic receptors, P2X7 deserves attention because it is involved in HE induced by CYP and, therefore, can be considered a therapeutic target. The objective of this study was to investigate the potential therapeutic effect of the quinovic acid glycosides purified fraction (QAPF) from U. tomentosa in the mouse model of CYP-induced HE. Pretreatment with QAPF not only had a protective effect on HE-induced urothelial damage (edema, hemorrhage and bladder wet weight) but was also able to control visceral pain, decrease IL-1β levels and down-regulates P2X7 receptors, most likely by inhibit the neutrophils migration to the bladder. This research clearly demonstrates the promising anti-inflammatory properties of QAPF, supporting its use as complementary therapy. QAPF represents a promising therapeutic option for this pathological condition.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Fabrícia
authors.initials: F
authors.lastname: Dietrich
authors.affiliation: Laboratório de Farmacologia Aplicada, Faculdade de Farmácia, PUCRS, Porto Alegre, RS, Brazil.
authors.forename: Jerônimo
authors.initials: J
authors.lastname: Pietrobon Martins
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Samuel
authors.initials: S
authors.lastname: Kaiser
authors.affiliation: Laboratório de Farmacologia Aplicada, Faculdade de Farmácia, PUCRS, Porto Alegre, RS, Brazil; Instituto de Toxicologia e Farmacologia, PUCRS, Porto Alegre, RS, Brazil.
authors.forename: Rodrigo Braccini
authors.initials: RB
authors.lastname: Madeira Silva
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Liliana
authors.initials: L
authors.lastname: Rockenbach
authors.affiliation: Faculdade de Medicina, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Albano Edelweiss
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: George González
authors.initials: GG
authors.lastname: Ortega
authors.affiliation: Laboratório de Farmacologia Aplicada, Faculdade de Farmácia, PUCRS, Porto Alegre, RS, Brazil; Instituto de Toxicologia e Farmacologia, PUCRS, Porto Alegre, RS, Brazil.
authors.forename: Fernanda Bueno
authors.initials: FB
authors.lastname: Morrone
authors.affiliation: Instituto de Toxicologia e Farmacologia, PUCRS, Porto Alegre, RS, Brazil; Faculdade de Odontologia, PUCRS, Porto Alegre, RS, Brazil.
authors.forename: Maria Martha
authors.initials: MM
authors.lastname: Campos
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil; Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Ana Maria Oliveira
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D020948:Cat's Claw / Q000737:chemistry*; D003520:Cyclophosphamide / Q000009:adverse effects*; D003556:Cystitis / Q000139:chemically induced / Q000150:complications* / Q000188:drug therapy* / Q000503:physiopathology; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006470:Hemorrhage / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D059225:Nociception / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001743:Urinary Bladder / Q000187:drug effects; D014781:Viscera / Q000187:drug effects
chemical_list: D006027:Glycosides; D053583:Interleukin-1beta; D020011:Protective Agents; D058486:Receptors, Purinergic P2X7; D014315:Triterpenes; D003520:Cyclophosphamide; C472605:quinovic acid; D009195:Peroxidase