title: Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time.
abstract: The immuno-PCR (IPCR) assay combines the versatility and robustness of immunoassays with the exponential signal amplification power of the polymerase chain reaction (PCR). Typically, IPCR allows a 10-1,000-fold increase in sensitivity over the analogous enzyme-linked immunosorbent assay (ELISA). This is achieved by replacing the signal-producing antibody-enzyme conjugate of an ELISA with an antibody-DNA conjugate that serves as a marker for PCR amplification. The amplification power of the PCR allows for the detection of even single molecules of nucleic acid templates, making it well suited for a broad range of applications. Here, we describe the application of an IPCR assay for detection of trace amount of antigens using ricin as an example.
authors.affiliation: Foodborne Toxin Detection and Prevention Research Unit (FTDP), Western Regional Research Center (WRRC), Pacific West Area (PWA), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 800 Buchanan St., Albany, CA, 94710, USA, xiaohua.he@ars.usda.gov.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: He
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Patfield
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D000941:Antigens / Q000032:analysis*; D001710:Biotin / Q000737:chemistry; D002021:Buffers; D004247:DNA / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012276:Ricin / Q000032:analysis*; D012278:Ricinus / Q000737:chemistry; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D002021:Buffers; D018796:Immunoconjugates; D001710:Biotin; D004247:DNA; D012276:Ricin; D019809:Streptavidin; D004262:DNA Restriction Enzymes