title: [Expression of interferon-induced protein with tetratricopetide repeats 1 and liver cell apoptosis in  mice with severe burns].
abstract: OBJECTIVE
  To explore the relationship between the expression of interferon-induced protein with tetratricopetide repeats 1 (IFIT1) and liver cell apoptosis in the acute stress period after severe burns.
  
  
  METHODS
  A total of 25 C57/129 adult mice were randomly divided into the normal control group (0 h) and the groups at 1, 6, 12 or 24 after severe burns (n=5 per group). A model with third degree (20% of the total body surface area) burn injury was established and then liver tissues were taken. IFIT1 expression was examined by Western blot. The expression of caspase-3 and -8 was measured by immunohistochemistry. Liver cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL).
  
  
  RESULTS
  After burns, IFIT1 expression was increased at 1 h, which reached the highest level at  6 h followed by a decrease at 12 h, which reached minimum level at 24 h. The differences between groups were significant (P<0.01). The caspase-3 and -8 levels significantly increased after burns in a time-dependent manner (P<0.01). Although at 0 h and 1 h there was no significant increase in liver cell apoptosis, the increase reached significance from 6 h to 24 h (P<0.01).
  
  
  CONCLUSIONS
  The increase in IFIT1 expression after severe burns promotes liver cell apoptosis.
authors.affiliation: Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000; State Key Laboratory of Trauma, Burns and Combined Injury; Institute of Combined Injury, College of Military Preventive Medicine, Th ird Military Medical University, Chongqing 400038,China.
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology, Affi liated Central Hospital of Xinxiang Medical University, Xinxiang Henan 453000, China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury; Institute of Combined Injury, College of Military Preventive Medicine, Th ird Military Medical University, Chongqing 400038,China.
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury; Institute of Combined Injury, College of Military Preventive Medicine, Th ird Military Medical University, Chongqing 400038,China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000,China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000,China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002056:Burns / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology*; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000166:cytology; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D016601:RNA-Binding Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C092573:Ifit1 protein, mouse; D016601:RNA-Binding Proteins; C505409:Casp3 protein, mouse; C505526:Casp8 protein, mouse; D053148:Caspase 3; D053181:Caspase 8