title: The auxin transporter, OsAUX1, is involved in primary root and root hair elongation and in Cd stress responses in rice (Oryza sativa L.).
abstract: Auxin and cadmium (Cd) stress play critical roles during root development. There are only a few reports on the mechanisms by which Cd stress influences auxin homeostasis and affects primary root (PR) and lateral root (LR) development, and almost nothing is known about how auxin and Cd interfere with root hair (RH) development. Here, we characterize rice osaux1 mutants that have a longer PR and shorter RHs in hydroponic culture, and that are more sensitive to Cd stress compared to wild-type (Dongjin). OsAUX1 expression in root hair cells is different from that of its paralogous gene, AtAUX1, which is expressed in non-hair cells. However, OsAUX1, like AtAUX1, localizes at the plasma membrane and appears to function as an auxin tranporter. Decreased auxin distribution and contents in the osaux1 mutant result in reduction of OsCyCB1;1 expression and shortened PRs, LRs and RHs under Cd stress, but may be rescued by treatment with the membrane-permeable auxin 1-naphthalene acetic acid. Treatment with the auxin transport inhibitors 1-naphthoxyacetic acid and N-1-naphthylphthalamic acid increased the Cd sensitivity of WT rice. Cd contents in the osaux1 mutant were not altered, but reactive oxygen species-mediated damage was enhanced, further increasing the sensitivity of the osaux1 mutant to Cd stress. Taken together, our results indicate that OsAUX1 plays an important role in root development and in responses to Cd stress.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: ChenLiang
authors.initials: C
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: ChenDong
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Chenjia
authors.initials: C
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Suikang
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: YunLong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Chuanyou
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.affiliation: Department of Biology - Plant Biology, University of Fribourg, Rue Albert Gockel 3, CH-1700, Fribourg, Switzerland.
authors.forename: Bibek
authors.initials: B
authors.lastname: Aryal
authors.affiliation: Department of Biology - Plant Biology, University of Fribourg, Rue Albert Gockel 3, CH-1700, Fribourg, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Geisler
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: De An
authors.initials: de A
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: YanHua
authors.initials: Y
authors.lastname: Qi
mesh_terms: D001692:Biological Transport; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006016:Glycolates / Q000494:pharmacology; D018527:Hydroponics / Q000379:methods; D007210:Indoleacetic Acids / Q000378:metabolism; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000502:physiology*; D010797:Phthalimides / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D006016:Glycolates; D007210:Indoleacetic Acids; D010797:Phthalimides; D010940:Plant Proteins; D002104:Cadmium; C002687:alpha-naphthylphthalamic acid; C018610:1-naphthoxyacetic acid
keywords: Cd stress; OsAUX1; Primary root; auxin; rice (Oryza sativa L.); root hair