title: Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies.
abstract: We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.
authors.affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK; Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Isaac
authors.initials: I
authors.lastname: Vizcaino-Caston
authors.affiliation: School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Kelly
authors.affiliation: School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Annabel V L
authors.initials: AVL
authors.lastname: Fitzgerald
authors.affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Leeke
authors.affiliation: School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Mike
authors.initials: M
authors.lastname: Jenkins
authors.affiliation: School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK; Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. Electronic address: t.w.overton@bham.ac.uk.
authors.forename: Tim W
authors.initials: TW
authors.lastname: Overton
mesh_terms: D002152:Calorimetry, Differential Scanning; D002725:Chloroform / Q000737:chemistry; D020118:Cupriavidus necator / Q000737:chemistry* / Q000166:cytology; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry* / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D054813:Polyhydroxyalkanoates; D012997:Solvents; D002725:Chloroform; D004121:Dimethyl Sulfoxide
keywords: Bioplastic; Dimethyl sulphoxide; Polyhydroxyalkanoate; Solvent extraction