title: Anticancer and Anti-Inflammatory Activity of Probiotic Lactococcus lactis NK34.
abstract: The anticancer and anti-inflammatory activities of probiotic Lactococcus lactis NK34 were demonstrated. Treatment of cancer cells such as SK-MES-1, DLD-1, HT-29, LoVo, AGS, and MCF-7 cells with 10(6) CFU/well of L. lactis NK34 resulted in strong inhibition of proliferation (>77% cytotoxicity, p < 0.05). The anti-inflammatory activity of L. lactis NK34 was also demonstrated in lipopolysaccharide-induced RAW 264.7 cells, where the production of nitric oxide and proinflammatory cytokines (tumor necrosis factor-α, interleukin-18, and cyclooxygenase-2) was reduced. These results suggest that L. lactis NK34 could be used as a probiotic microorganism to inhibit the proliferation of cancer cells and production of proinflammatory cytokines.
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Kyoung Jun
authors.initials: KJ
authors.lastname: Han
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Na-Kyoung
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Department of Food Science, Sun Moon University, Asan 336-708, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.|Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D013294:Lactococcus lactis / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D016207:Cytokines; D009569:Nitric Oxide
keywords: Lactococcus lactis; Probiotic; anti-inflammatory activity; anticancer activity; cytokine