title: Comparing Two Intestinal Porcine Epithelial Cell Lines (IPECs): Morphological Differentiation, Function and Metabolism.
abstract: The pig shows genetical and physiological resemblance to human, which predestines it as an experimental animal model especially for mucosal physiology. Therefore, the intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2)--spontaneously immortalised cell lines from the porcine intestine--are important tools for studying intestinal function. A microarray (GeneChip Porcine Genome Array) was performed to compare the genome wide gene expression of IPECs. Different significantly up-regulated pathways were identified, like "lysosome", "pathways in cancer", "regulation of actin cytoskeleton" and "oxidative phosphorylation" in IPEC-J2 in comparison to IPEC-1. On the other hand, "spliceosome", "ribosome", "RNA-degradation" and "tight junction" are significantly down-regulated pathways in IPEC-J2 in comparison to IPEC-1. Examined pathways were followed up by functional analyses. ATP-, oxygen, glucose and lactate-measurement provide evidence for up-regulation of oxidative phosphorylation in IPEC-J2. These cells seem to be more active in their metabolism than IPEC-1 cells due to a significant higher ATP-content as well as a higher O2- and glucose-consumption. The down-regulated pathway "ribosome" was followed up by measurement of RNA- and protein content. In summary, IPEC-J2 is a morphologically and functionally more differentiated cell line in comparison to IPEC-1. In addition, IPEC-J2 cells are a preferential tool for in vitro studies with the focus on metabolism.
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Nossol
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Anicò
authors.initials: A
authors.lastname: Barta-Böszörményi
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kahlert
authors.affiliation: Leibniz Institute of Neurobiology, 39120, Magdeburg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Zuschratter
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Heidi
authors.initials: H
authors.lastname: Faber-Zuschratter
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Reinhardt
authors.affiliation: Leibniz Institute of Farm Animal Biology (FBN) Dummerstorf, 18196, Dummerstorf, Germany.
authors.forename: Siriluk
authors.initials: S
authors.lastname: Ponsuksili
authors.affiliation: Leibniz Institute of Farm Animal Biology (FBN) Dummerstorf, 18196, Dummerstorf, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Diesing
authors.affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, 39120, Magdeburg, Germany.
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Rothkötter
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D048430:Cell Shape / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D007422:Intestines / Q000166:cytology*; D010766:Phosphorylation; D013552:Swine; D015854:Up-Regulation