title: Skin Biopsy and Patient-Specific Stem Cell Lines.
abstract: The generation of patient-specific induced pluripotent stem (iPS) cells permits the development of next-generation patient-specific systems biology models reflecting personalized genomics profiles to better understand pathophysiology. In this chapter, we describe how to create a patient-specific iPS cell line. There are three major steps: (1) performing a skin biopsy procedure on the patient; (2) extracting human fibroblast cells from the skin biopsy tissue; and (3) reprogramming patient-specific fibroblast cells into the pluripotent stem cell stage.
authors.affiliation: Barbara & Donald Jonas Laboratory of Stem Cell and Regenerative Medicine, Department of Ophthalmology, Columbia University, New York, NY, USA.|Bernard & Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University, New York, NY, USA.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, NY, USA.
authors.forename: Huy V
authors.initials: HV
authors.lastname: Nguyen
authors.affiliation: New York Presbyterian Hospital/Columbia University Medical Center, New York, NY, USA. sht2@columbia.edu.|Department of Pathology and Cell Biology and Institute, Columbia University, New York, NY, USA. sht2@columbia.edu.|Edward Harkness Eye Institute, 160 Fort Washington Avenue, Research Annex, Room 513, New York, NY, 10032, USA. sht2@columbia.edu.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Tsang
mesh_terms: D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D001706:Biopsy; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D029082:Sendai virus / Q000235:genetics; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013724:Teratoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019076:Transgenes
chemical_list: D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489427:MYC protein, human; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: Fibroblast; Human iPS; Reprogramming; Sendai virus; Skin biopsy