title: Lack of Connection Between Midgut Cell Autophagy Gene Expression and BmCPV Infection in the Midgut of Bombyx mori.
abstract: Autophagy is associated with multiple biological processes and has protective and defensive functions with respect to immunity, inflammation, and resistance to microbial infection. In this experiment, we wished to investigate whether autophagy is a factor in the midgut cell response of Bombyx mori to infection by the B. mori cytoplasmic polyhedrosis virus (BmCPV). Our results indicated that the expression of three autophagy-related genes (BmAtg8, BmAtg5, and BmAtg7) in the midgut did not change greatly after BmCPV infection in B. mori. Basal ATG8/ATG8PE protein expression was detected in different B. mori tissues by using western blot analysis. Immunohistochemistry showed that the ATG8/ATG8PE proteins were located mainly in the cytoplasm. ATG8/ATG8PE protein levels decreased at 12 and 16 h after BmCPV infection. Our results indicate that autophagy responded slightly to BmCPV infection, but could not prevent the invasion and replication of the virus.
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forest University, Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forest University, Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Suli
authors.initials: S
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forest University, Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Yongpeng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forest University, Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forest University, Xinong Road, Yangling, Shaanxi, 712100, China qyh@nwsuaf.edu.cn fjiao@nwsuaf.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Qian
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forest University, Xinong Road, Yangling, Shaanxi, 712100, China qyh@nwsuaf.edu.cn fjiao@nwsuaf.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiao
mesh_terms: D000818:Animals; D001343:Autophagy; D012831:Bombyx / Q000235:genetics / Q000502:physiology* / Q000821:virology*; D004064:Digestive System / Q000821:virology; D004068:Digestive System Physiological Phenomena; D005786:Gene Expression Regulation; D012087:Reoviridae / Q000502:physiology*
keywords: autophagy; cytoplasmic polyhedrosis virus (CPV); immunohistochemistry; silkworm (Bombyx mori) midgut; western blotting