title: Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats.
abstract: Collagen tripeptide (CTP) is a collagen-derived compound containing a high concentration of tripeptides with a Gly-X-Y sequence. In this study, the concentrations and metabolites of CTP were monitored in rat plasma after its administration. We performed a quantitative analysis using high-performance liquid chromatography tandem mass spectrometry according to the isotopic dilution method with stable isotopes. We confirmed that the tripeptides Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp were transported into the plasma. Dipeptides, which are generated by degradation of the N- or C-terminus of the tripeptides Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp, were also present in plasma. The plasma kinetics for peroral and intraperitoneal administration was similar. In addition, tripeptides and dipeptides were detected in no-administration rat blood. The pharmacokinetics were monitored in rats perorally administered with Gly-[(3)H]Pro-Hyp. Furthermore, CTP was incorporated into tissues including skin, bone, and joint tissue. Thus, administering collagen as tripeptides enables efficient absorption of tripeptides and dipeptides.
authors.affiliation: a Central Research Institute, Jellice Co.,Ltd. , Tagajo , Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: a Central Research Institute, Jellice Co.,Ltd. , Tagajo , Japan.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Hayasaka
authors.affiliation: b Medical Care Proteomics Biotechnology Co.,Ltd. , Kahoku , Japan.
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Deguchi
authors.affiliation: c Department of Anatomy , Kanagawa Dental College , Yokosuka , Japan.
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Okudera
authors.affiliation: d Department of Oral Anatomy , Kanagawa Dental College , Yokosuka , Japan.
authors.forename: Toshitake
authors.initials: T
authors.lastname: Furusawa
authors.affiliation: a Central Research Institute, Jellice Co.,Ltd. , Tagajo , Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sakai
mesh_terms: D065966:Absorption, Physicochemical; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry*; D007274:Injections, Intraperitoneal; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D009842:Oligopeptides / Q000008:administration & dosage* / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009842:Oligopeptides; D003094:Collagen
keywords: Gly-Pro-Hyp; HPLC-MS/MS; collagen tripeptide; plasma kinetics