title: Extracellular vesicles from women with breast cancer promote an epithelial-mesenchymal transition-like process in mammary epithelial cells MCF10A.
abstract: Extracellular vesicles (EVs) mediate many stages of tumor progression including angiogenesis, escape from immune surveillance, and extracellular matrix degradation. We studied whether EVs from plasma of women with breast cancer are able to induce an epithelial-mesenchymal transition (EMT) process in mammary epithelial cells MCF10A. Our findings demonstrate that EVs from plasma of breast cancer patients induce a downregulation of E-cadherin expression and an increase of vimentin and N-cadherin expression. Moreover, EVs induce migration and invasion, as well as an increase of NFκB-DNA binding activity and MMP-2 and MMP-9 secretions. In summary, our findings demonstrate, for the first time, that EVs from breast cancer patients induce an EMT-like process in human mammary non-tumorigenic epithelial cells MCF10A.
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, Mexico, D.F., 07360, Mexico.
authors.forename: Octavio
authors.initials: O
authors.lastname: Galindo-Hernandez
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, Mexico, D.F., 07360, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gonzales-Vazquez
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, Mexico, D.F., 07360, Mexico.
authors.forename: Pedro
authors.initials: P
authors.lastname: Cortes-Reynosa
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, Mexico, D.F., 07360, Mexico.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Reyes-Uribe
authors.affiliation: Laboratorio de Genetica y Diagnostico Molecular, Unidad de Investigacion, Hospital Juarez de Mexico, Mexico, D.F., Mexico.
authors.forename: Sonia
authors.initials: S
authors.lastname: Chavez-Ocaña
authors.affiliation: Laboratorio de Genetica y Diagnostico Molecular, Unidad de Investigacion, Hospital Juarez de Mexico, Mexico, D.F., Mexico.
authors.forename: Octavio
authors.initials: O
authors.lastname: Reyes-Hernandez
authors.affiliation: Laboratorio de Genetica y Diagnostico Molecular, Unidad de Investigacion, Hospital Juarez de Mexico, Mexico, D.F., Mexico.
authors.forename: Mónica
authors.initials: M
authors.lastname: Sierra-Martinez
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, Mexico, D.F., 07360, Mexico. jperez@cell.cinvestav.mx.
authors.forename: Eduardo Perez
authors.initials: EP
authors.lastname: Salazar
mesh_terms: D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology; D002460:Cell Line; D058750:Epithelial-Mesenchymal Transition; D000067128:Extracellular Vesicles / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D042361:Mammary Glands, Human / Q000473:pathology*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010949:Plasma / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins
keywords: Breast cancer; EMT; Extracellular vesicles; MCF10A