title: The Polymerization of Aeromonas hydrophila AH-3 O-Antigen LPS: Concerted Action of WecP and Wzy.
abstract: The repeat units of heteropolymeric O antigen are synthesized at the cytosolic side of the inner bacterial membrane via the Wzx/Wzy-dependent assembly pathway. After being translocated across the membrane by Wzx, each repeat unit is polymerized by Wzy to form a glycan chain. In this study, we demonstrate the need of the corresponding enzyme transferring the initial HexNAc to undecaprenol phosphate (lipid carrier), together with the corresponding O-antigen polymerase (Wzy), to produce the Aeromonas hydrophila O:34-antigen. We suggest, the concerted action of WecA or P enzyme (UDP-HexNAc: polyprenol-P HexNAc-1-P transferase) and Wzy is involved in the mechanism responsible for the A. hydrophila O-antigen polymerization.
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 643, 08071, Barcelona, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 643, 08071, Barcelona, Spain.
authors.forename: Victor
authors.initials: V
authors.lastname: Gonzalez
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 643, 08071, Barcelona, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D016980:Aeromonas hydrophila / Q000201:enzymology* / Q000276:immunology / Q000472:pathogenicity; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000502:physiology; D053898:Biosynthetic Pathways; D045744:Cell Line, Tumor; D005260:Female; D016695:Glycosyltransferases / Q000502:physiology; D006801:Humans; D051379:Mice; D019081:O Antigens / Q000096:biosynthesis* / Q000276:immunology; D017686:Oncorhynchus mykiss; D058105:Polymerization; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D019081:O Antigens; D016695:Glycosyltransferases