title: Effects of moderate- and high-intensity chronic exercise on brain-derived neurotrophic factor expression in fast and slow muscles.
abstract: BACKGROUND
  Brain-derived neurotrophic factor (BDNF) protein expression is sensitive to cellular activity. In the sedentary state, BDNF expression is affected by the muscle phenotype.
  
  
  METHODS
  Eighteen Wistar rats were divided into the following 3 groups: sedentary (S); moderate-intensity training (MIT); and high-intensity training (HIT). The training protocol lasted 8 weeks. Forty-eight hours after training, total RNA and protein levels in the soleus and plantaris muscles were obtained.
  
  
  RESULTS
  In the plantaris, the BDNF protein level was lower in the HIT than in the S group (P < 0.05). A similar effect was found in the soleus (without significant difference). In the soleus, higher Bdnf mRNA levels were found in the HIT group (P < 0.001 vs. S and MIT groups). In the plantaris muscle, similar Bdnf mRNA levels were found in all groups.
  
  
  CONCLUSIONS
  These results indicate that high-intensity chronic exercise reduces BDNF protein level in fast muscles and increases Bdnf mRNA levels in slow muscles.
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 965 Ave. 25 de Julio, Col. Villas San Sebastián, Colima, 28045, México.
authors.forename: Alberto
authors.initials: A
authors.lastname: Jiménez-Maldonado
authors.affiliation: Facultad de Medicina, Universidad de Colima, Colima, México.
authors.forename: Joel
authors.initials: J
authors.lastname: Cerna-Cortés
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 965 Ave. 25 de Julio, Col. Villas San Sebastián, Colima, 28045, México.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Castro-Rodríguez
authors.affiliation: Facultad de Medicina, Universidad de Colima, Colima, México.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Montero
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 965 Ave. 25 de Julio, Col. Villas San Sebastián, Colima, 28045, México.
authors.forename: Jesús
authors.initials: J
authors.lastname: Muñiz
authors.affiliation: Facultad de Medicina, Universidad de Colima, Colima, México.
authors.forename: Alejandrina
authors.initials: A
authors.lastname: Rodríguez-Hernández
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 965 Ave. 25 de Julio, Col. Villas San Sebastián, Colima, 28045, México.
authors.forename: Mónica
authors.initials: M
authors.lastname: Lemus
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 965 Ave. 25 de Julio, Col. Villas San Sebastián, Colima, 28045, México.
authors.forename: Elena Roces
authors.initials: ER
authors.lastname: De Álvarez-Buylla
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger
keywords: BDNF expression; brain-derived neurotrophic factor; chronic exercise; gene; skeletal muscle