title: Characterization of a novel cometabolic degradation carbazole pathway by a phenol-cultivated Arthrobacter sp. W1.
abstract: Arthrobacter sp. W1 was used to characterize the pathways involved in cometabolic degradation of carbazole (CA) with phenol as the primary substrate. To clarify the upper pathway of cometabolic degradation CA, Escherichia coli strain BL21 expressing phenol hydroxylase from strain W1 (PHIND) was investigated to degrade CA. Firstly, CA was initially monohydroxylated at C-2 and C-4 positions to produce 2- and 4-hydroxycarbazole, followed by successively hydroxylated to the corresponding 1,2- and 3,4-dihydroxycarbazole, of which 3,4-dihydroxycarbazole was unequivocally identified for the first time. To characterize the downstream cometabolic degradation CA pathway, purified 3,4-dihydroxycarbazole was used as the substrate for phenol-grown W1, and a series of novel indole derivatives were identified. These results suggested that a novel pathway of CA catabolism was employed by strain W1 via a successive hydroxylation and meta-cleavage pathway. These findings provide new insights into the cometabolic degradation CA process and have potential applications in biotechnology and bioremediation.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; School of Life Science, Liaoning Normal University, Dalian 116081, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Shi
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: hit_dlut@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
mesh_terms: D001173:Arthrobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D002227:Carbazoles / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D019800:Phenol / Q000378:metabolism*
chemical_list: D002227:Carbazoles; C041514:carbazole; D019800:Phenol; C571814:4-hydroxycarbazole; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase
keywords: Arthrobacter sp. W1; Carbazole; Cometabolic degradation; Pathway