title: HISTONE DEACETYLASE6-Defective Mutants Show Increased Expression and Acetylation of ENHANCER OF TRIPTYCHON AND CAPRICE1 and GLABRA2 with Small But Significant Effects on Root Epidermis Cellular Pattern.
abstract: Cellular patterning in the Arabidopsis (Arabidopsis thaliana) root epidermis is dependent on positional information, the transmission of which involves histone acetylation. Here, we report that HISTONE DEACETYLASE6 (HDA6) has significant effects on this cellular patterning. Mutation of HDA6 led to ectopic hair cells in the nonhair positions of root epidermis in Arabidopsis, based on an analysis of paraffin sections stained with Toluidine Blue. While HDA6 was present throughout the root tip, epidermis-specific complementation with HDA6 could rescue the hda6 phenotype. Both transcript levels and expression patterns of ENHANCER OF TRIPTYCHON AND CAPRICE1 (ETC1) and GLABRA2 (GL2) in the root tip were affected in hda6. Consistent with these changes in expression, HDA6 directly bound to the promoter regions of ETC1 and GL2, and acetylation of histone H3 on these promoter regions and acetylation of histone H4 on the ETC1 promoter region was increased in the hda6 mutant. Taken together, these results indicate that HDA6 affects the cellular patterning of Arabidopsis root epidermis through altering the histone acetylation status of ETC1 and GL2 promoters and thereby affects the expression of these two components of the core transcription factor network determining epidermal cell fates. Our findings thus provide new insights into the role of histone acetylation in root epidermis cell patterning.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Science, Peking University, Beijing 100871, China; andNational Center of Plant Gene Research, Beijing 100871, China.
authors.forename: Dong-Xu
authors.initials: DX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Science, Peking University, Beijing 100871, China; andNational Center of Plant Gene Research, Beijing 100871, China.
authors.forename: Wen-Qian
authors.initials: WQ
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Science, Peking University, Beijing 100871, China; andNational Center of Plant Gene Research, Beijing 100871, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Science, Peking University, Beijing 100871, China; andNational Center of Plant Gene Research, Beijing 100871, China shunongb@pku.edu.cn.
authors.forename: Shu-Nong
authors.initials: SN
authors.lastname: Bai
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D009154:Mutation; D019441:Plant Epidermis / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D049452:Green Fluorescent Proteins; D006655:Histone Deacetylases; D018398:Homeodomain Proteins; C485326:ETC1 protein, Arabidopsis; C089991:GL2 protein, Arabidopsis; C432452:AT5G63110 protein, Arabidopsis