title: Unique gene program of rat small resistance mesenteric arteries as revealed by deep RNA sequencing.
abstract: Deep sequencing of RNA samples from rat small mesenteric arteries (MA) and aorta (AO) identified common and unique features of their gene programs. ~5% of mRNAs were quantitatively differentially expressed in MA versus AO. Unique transcriptional control in MA smooth muscle is suggested by the selective or enriched expression of transcription factors Nkx2-3, HAND2, and Tcf21 (Capsulin). Enrichment in AO of PPAR transcription factors and their target genes of mitochondrial function, lipid metabolism, and oxidative phosphorylation is consistent with slow (oxidative) tonic smooth muscle. In contrast MA was enriched in contractile and calcium channel mRNAs suggestive of components of fast (glycolytic) phasic smooth muscle. Myosin phosphatase regulatory subunit paralogs Mypt1 and p85 were expressed at similar levels, while smooth muscle MLCK was the only such kinase expressed, suggesting functional redundancy of the former but not the latter in accordance with mouse knockout studies. With regard to vaso-regulatory signals, purinergic receptors P2rx1 and P2rx5 were reciprocally expressed in MA versus AO, while the olfactory receptor Olr59 was enriched in MA. Alox15, which generates the EDHF HPETE, was enriched in MA while eNOS was equally expressed, consistent with the greater role of EDHF in the smaller arteries. mRNAs that were not expressed at a level consistent with impugned function include skeletal myogenic factors, IKK2, nonmuscle myosin, and Gnb3. This screening analysis of gene expression in the small mesenteric resistance arteries suggests testable hypotheses regarding unique aspects of small artery function in the regional control of blood flow.
authors.affiliation: Department of Medicine, Division of Cardiovascular Medicine, University of Maryland-Baltimore, Baltimore, Maryland, 21201.
authors.forename: John J
authors.initials: JJ
authors.lastname: Reho
authors.affiliation: Institute for Genome Sciences, University of Maryland-Baltimore, Baltimore, Maryland, 21201.
authors.forename: Amol
authors.initials: A
authors.lastname: Shetty
authors.affiliation: Department of Medicine, Division of Cardiovascular Medicine, University of Maryland-Baltimore, Baltimore, Maryland, 21201.
authors.forename: Rachael P
authors.initials: RP
authors.lastname: Dippold
authors.affiliation: Institute for Genome Sciences, University of Maryland-Baltimore, Baltimore, Maryland, 21201.
authors.forename: Anup
authors.initials: A
authors.lastname: Mahurkar
authors.affiliation: Department of Medicine, Division of Cardiovascular Medicine, University of Maryland-Baltimore, Baltimore, Maryland, 21201 sfisher1@medicine.umaryland.edu.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Fisher
keywords: Aorta; Nkx2‐3; mesenteric artery; mitochondria; vascular smooth muscle