title: Phenolic, flavonoid contents, anticholinesterase and antioxidant evaluation of Iris germanica var; florentina.
abstract: This study was designed to investigate antioxidant and anticholinesterase potential of Iris germanica var; florentina. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory potential of plant samples were investigated by Ellman's assay. Antioxidant activity was performed using DPPH, H2O2 and ABTS free radical scavenging assays. Total phenolics and flavonoids contents were expressed in mg GAE/g dry weight and mg RTE/g, respectively. In AChE inhibition assay, Ig.Fl, Ig.Sp and Ig.Cf fractions exhibited highest activity with IC50 values of < 0.1, 5.64 and 19 μg/mL, respectively. In BChE inhibitory assay, Ig.Fl, Ig.Sp, Ig.Cf and Ig.Cr were most active with IC50 of < 0.1, < 0.1, 31 and 78 μg/mL, respectively. In DPPH assay, Ig.Fl and Ig.Cf exhibited highest inhibition of free radicals, 80.52% (IC50 = 9 μg/mL) and 78.30% (IC50 = 8 μg/mL), respectively. In ABTS assay Ig.Cr, Ig.Cf, Ig.Fl and Ig.Sp exhibited IC50 values of < 0.1, 2, 2 and 3 μg/mL, respectively.
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Farhat
authors.initials: F
authors.lastname: Ullah
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ayaz
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Sadiq
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Abid
authors.initials: A
authors.lastname: Hussain
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Imran
authors.affiliation: a Department of Pharmacy , University of Malakand , Chakdara, Dir Lower, Khyber Pakhtoonkhwa 18000 , Pakistan.
authors.forename: Anwar
authors.initials: A
authors.lastname: Zeb
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000032:analysis; D005609:Free Radicals / Q000378:metabolism; D020128:Inhibitory Concentration 50; D031323:Iris Plant / Q000737:chemistry*; D010636:Phenols / Q000032:analysis
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D005419:Flavonoids; D005609:Free Radicals; D010636:Phenols; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase
keywords: ABTS; Acetylcholinesterase (AChE); DPPH; Ellman’s assay; H2O2; Iris germanica; butyrylcholinesterase (BChE)