title: Optical Sectioning and High Resolution in Single-Slice Structured Illumination Microscopy by Thick Slice Blind-SIM Reconstruction.
abstract: The microscope image of a thick fluorescent sample taken at a given focal plane is plagued by out-of-focus fluorescence and diffraction limited resolution. In this work, we show that a single slice of Structured Illumination Microscopy (two or three beam SIM) data can be processed to provide an image exhibiting tight sectioning and high transverse resolution. Our reconstruction algorithm is adapted from the blind-SIM technique which requires very little knowledge of the illumination patterns. It is thus able to deal with illumination distortions induced by the sample or illumination optics. We named this new algorithm thick slice blind-SIM because it models a three-dimensional sample even though only a single two-dimensional plane of focus was measured.
authors.affiliation: Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller University Jena, Jena, Germany; Leibniz-Institute of Photonic Technology, Jena, Germany.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Jost
authors.affiliation: Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller University Jena, Jena, Germany; Leibniz-Institute of Photonic Technology, Jena, Germany.
authors.forename: Elen
authors.initials: E
authors.lastname: Tolstik
authors.affiliation: Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller University Jena, Jena, Germany; Leibniz-Institute of Photonic Technology, Jena, Germany.
authors.forename: Polina
authors.initials: P
authors.lastname: Feldmann
authors.affiliation: Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller University Jena, Jena, Germany; Leibniz-Institute of Photonic Technology, Jena, Germany; Carl Zeiss AG, Corporate Research and Technology, Jena, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Wicker
authors.affiliation: Institut Fresnel, CNRS, Aix-Marseille Université, Marseille, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Sentenac
authors.affiliation: Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller University Jena, Jena, Germany; Leibniz-Institute of Photonic Technology, Jena, Germany; King's College London, Randall Division, London, United Kingdom.
authors.forename: Rainer
authors.initials: R
authors.lastname: Heintzmann
mesh_terms: D000199:Actins / Q000032:analysis; D000465:Algorithms; D016477:Artifacts; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008029:Lighting; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D051419:Paxillin / Q000032:analysis
chemical_list: D000199:Actins; D051419:Paxillin