title: MoS2 nanosheet-based fluorescent biosensor for protein detection via terminal protection of small-molecule-linked DNA and exonuclease III-aided DNA recycling amplification.
abstract: A new MoS2 nanosheet-based fluorescent biosensor for protein detection is developed. This method combines the terminal protection of small-molecule-linked DNA (TPSMLD) and exonuclease III (Exo III)-aided DNA recycling amplification to convert protein assay into the highly sensitive detection of DNA. Taking the streptavidin (SA)-biotin system as a model, a detection limit of 0.67 ng mL(-1) SA is obtained with a good selectivity. The study demonstrated here not only offers simple, sensitive and selective detection method for protein assay, but also will expand the application of the emerging 2D nanomaterials into biological assay.
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, 430064 Hubei Province, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Fenghong
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Qianchun
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Department of Product Processing and Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
authors.forename: Jiqu
authors.initials: J
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D004220:Disulfides / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D019809:Streptavidin / Q000032:analysis* / Q000097:blood
chemical_list: D004220:Disulfides; D001710:Biotin; D008982:Molybdenum; D004247:DNA; D019809:Streptavidin; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; C082964:molybdenum disulfide
keywords: Exonuclease III; Fluorescent biosensor; MoS(2) nanosheet; Protein detection; Terminal protection of small-molecule-linked DNA