title: Molecular cloning and expression analysis of the retinoid X receptor (RXR) gene in golden pompano Trachinotus ovatus fed Artemia nauplii with different enrichments.
abstract: The retinoid X receptors (RXRs) are involved in the skeletal development and other biological process such as blood vessel formation and metabolism. Partial sequences of RXRα and β genes were obtained, and their expressions were quantified on golden pompano Trachinotus ovatus at 28 days post hatching (DPH) to explore the molecular response to nutritional manipulation in fish larvae. As live food, Artemia nauplii were separately enriched with Nannochloropsis and Algamac 3080 and non-enriched Artemia nauplii (control) for fish feeding. The expressions of RXRs were detected in the embryos and fish larvae at early stages, suggesting that the skeletal development in golden pompano initiated before yolk re-sorption completion. Fish fed non-enriched Artemia nauplii ended up with higher jaw malformation. The highest specific growth rate was obtained when fish were fed with the Artemia nauplii enriched with Algamac 3080, and the lowest growth rate was observed when fish were fed with unenriched Artemia nauplii. The highest survival was obtained when fish were fed with non-enriched or Nannochloropsis-enriched Artemia nauplii. This study indicates that the use of enriched formula for Artemia nauplii can significantly affect the expression levels of RXRs and jaw malformation of golden pompano larvae, but there is no clear correlation between RXRs expressions and malformation rates when fish are subjected to nutrient challenge.
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, China.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Panlong
authors.initials: P
authors.lastname: Zheng
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China. zhenhua.ma@hotmail.com.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ma
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Jiang
authors.affiliation: School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, 5001, Australia.
authors.forename: Jian G
authors.initials: JG
authors.lastname: Qin
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001156:Artemia; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007569:Jaw Abnormalities; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics / Q000254:growth & development*; D047488:Retinoid X Receptors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D047488:Retinoid X Receptors
keywords: Artemia nauplii; Enrichment; Gene expression; Golden pompano larvae; Jaw malformation; RXRα; RXRβ