title: Estimation of Salivary Glucose and Glycogen Content in Exfoliated Buccal Mucosal Cells of Patients with Type II Diabetes Mellitus.
abstract: BACKGROUND
  Diabetes mellitus is a common metabolic disorder which shows an increasing incidence worldwide. Constant monitoring of blood glucose in diabetic patient is required which involves painful invasive techniques. Saliva is gaining acceptance as diagnostic tool for various systemic diseases which can be collected noninvasively and by individuals with limited training.
  
  
  OBJECTIVE
  The aim of the present study was to analyse the possibility of using salivary glucose and glycogen content of buccal mucosal cells as a diagnostic marker in Type II Diabetes mellitus patients which can be considered as adjuvant diagnostic tool to the gold standards.
  
  
  METHODS
  Sample consists of 30 study and 30 control groups. Saliva was collected by passive drool method.Intravenous blood samples were collected for glucose estimation. Exfoliated buccal mucosal cells were collected from apparently normal buccal mucosa, smeared on dry glass slide and stained with PAS. Blood and salivary glucose are estimated by Glucose Oxidase endpoint method. For Glycogen estimation, number of PAS positive cells in fifty unfolded cells was analysed.
  
  
  RESULTS
  The results of the present study revealed a significant increase in the salivary glucose level and the number of PAS positive buccal mucosal cells in the diabetics than in the controls. The correlation between the fasting serum glucose and fasting salivary glucose and also that between fasting serum glucose and PAS positive cells was statistically significant. But the correlation between the staining intensity and fasting serum glucose was statistically insignificant.
  
  
  CONCLUSIONS
  With the results of the present study it is revealed that salivary glucose and PAS positive cells are increased in diabetics which can be considered as adjuvant diagnostic tool for Diabetes mellitus.
authors.affiliation: Professor and HOD, Department of Oral and Maxillofacial Pathology, Azeezia College of Dental Science & Research , Meeyannoor, Kerala, India .
authors.forename: Rathy
authors.initials: R
authors.lastname: Ravindran
authors.affiliation: Post Graduate Student, Department of Oral & Maxillofacial Pathology, Azeezia College of Dental Science & Research , Meeyannoor, Kerala, India .
authors.forename: Deepa Moothedathu
authors.initials: DM
authors.lastname: Gopinathan
authors.affiliation: Professor, Department of Oral & Maxillofacial Pathology, Azeezia College of Dental Science & Research , Meeyannoor, Kerala, India .
authors.forename: Sunil
authors.initials: S
authors.lastname: Sukumaran
keywords: Diagnostic tool; PAS positive cells; Saliva