title: Induction of human umbilical Wharton's jelly-derived mesenchymal stem cells toward motor neuron-like cells.
abstract: The most important property of stem cells from different sources is the capacity to differentiate into various cells and tissue types. However, problems including contamination, normal karyotype, and ethical issues cause many limitations in obtaining and using these cells from different sources. The cells in Wharton's jelly region of umbilical cord represent a pool source of primitive cells with properties of mesenchymal stem cells (MSCs). The aim of this study was to determine the potential of human Wharton's jelly-derived mesenchymal stem cells (WJMSCs) for differentiation to motor neuron cells. WJMSCs were induced to differentiate into motor neuron-like cells by using different signaling molecules and neurotrophic factors in vitro. Differentiated neurons were then characterized for expression of motor neuron markers including nestin, PAX6, NF-H, Islet 1, HB9, and choline acetyl transferase (ChAT) by quantitative reverse transcription PCR and immunocytochemistry. Our results showed that differentiated WJMSCs could significantly express motor neuron biomarkers in RNA and protein levels 15 d post induction. These results suggested that WJMSCs can differentiate to motor neuron-like cells and might provide a potential source in cell therapy for neurodegenerative disease.
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Bagher
authors.affiliation: Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Ebrahimi-Barough
authors.affiliation: Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Azami
authors.affiliation: Polymer Engineering Faculty, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Mirzadeh
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.|Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mansooreh
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
authors.affiliation: Tissue Engineering Division, Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nourani
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. joghataei.mt@iums.ac.ir.|Department of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, Iran. joghataei.mt@iums.ac.ir.|Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. joghataei.mt@iums.ac.ir.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Joghataei
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009046:Motor Neurons / Q000166:cytology* / Q000502:physiology; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D015415:Biomarkers; D005136:Eye Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C577484:NES protein, human; D064231:Nestin; D016900:Neurofilament Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; C051774:neurofilament protein H
keywords: Differentiation; Human umbilical Wharton’s jelly-derived MSCS; Motor neuron