title: Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation.
abstract: Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the putative causes for the collapse of this species. Among their possible effects, contaminants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. The aim of this study was to investigate the effects of organic and inorganic contaminants on the gonad development of wild female silver eels. Female silver eels from two sites with differing contamination levels were artificially matured. Transcriptomic analyses by means of a 1000 candidate gene cDNA microarray were performed on gonads after 11weeks of maturation to get insight into the mechanisms of toxicity of contaminants. The transcription levels of several genes, that were associated to the gonadosomatic index (GSI), were involved in mitotic cell division but also in gametogenesis. Genes associated to contaminants were mainly involved in the mechanisms of protection against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated fish.
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400 Talence, France; CNRS, EPOC, UMR 5805, F-33400 Talence, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Baillon
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400 Talence, France; CNRS, EPOC, UMR 5805, F-33400 Talence, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Oses
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400 Talence, France; CNRS, EPOC, UMR 5805, F-33400 Talence, France. Electronic address: f.pierron@epoc.u-bordeaux1.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Pierron
authors.affiliation: Irtsea, UR EABX, 50 avenue de Verdun-Gazinet, 33612 Cestas, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Bureau du Colombier
authors.affiliation: Institut National de la Recherche Scientifique, Centre Eau Terre Environnement, 490 de la Couronne, Québec, Québec G1K 9A9, Canada.
authors.forename: Antoine
authors.initials: A
authors.lastname: Caron
authors.affiliation: Muséum National d'Histoire Naturelle, UMR BOREA UPMC, CNRS 7208, IRD 207, UCBN, 7 rue Cuvier CP 32, F-75231 Paris, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Normandeau
authors.affiliation: Irtsea, UR EABX, 50 avenue de Verdun-Gazinet, 33612 Cestas, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lambert
authors.affiliation: Institut National de la Recherche Scientifique, Centre Eau Terre Environnement, 490 de la Couronne, Québec, Québec G1K 9A9, Canada.
authors.forename: Patrice
authors.initials: P
authors.lastname: Couture
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400 Talence, France; CNRS, EPOC, UMR 5805, F-33400 Talence, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Labadie
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400 Talence, France; CNRS, EPOC, UMR 5805, F-33400 Talence, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.affiliation: Muséum National d'Histoire Naturelle, UMR BOREA UPMC, CNRS 7208, IRD 207, UCBN, 7 rue Cuvier CP 32, F-75231 Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dufour
authors.affiliation: Département de biologie, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Québec G1V 0A6, Canada.
authors.forename: Louis
authors.initials: L
authors.lastname: Bernatchez
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400 Talence, France; CNRS, EPOC, UMR 5805, F-33400 Talence, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Baudrimont
mesh_terms: D000811:Anguilla / Q000378:metabolism; D000818:Animals; D001822:Body Burden; D056727:Endangered Species; D005260:Female; D020869:Gene Expression Profiling; D006846:Hydrocarbons, Halogenated / Q000032:analysis* / Q000493:pharmacokinetics / Q000633:toxicity; D019216:Metals, Heavy / Q000032:analysis* / Q000493:pharmacokinetics / Q000633:toxicity; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D012733:Sex Differentiation; D012741:Sexual Maturation / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D006846:Hydrocarbons, Halogenated; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical
keywords: Anguilla anguilla; Contamination; Gonad development; Silver stage; Transcriptomic