title: MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma.
abstract: OBJECTIVE
  Altered microRNA expression has been found in many cancer types, including laryngeal squamous cell carcinoma (LSCC). We investigated the association of LSCC-related miR-34c-5p with disease-free survival and overall survival.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  Expression levels of miR-34c-5p were detected in 90 LSCC formalin-fixed paraffin-embedded tissues by reverse-transcription quantitative polymerase chain reaction. Overall survival and disease-free survival were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using Cox proportional hazard analysis.
  
  
  RESULTS
  A downregulation of miR-34c-5p expression significantly correlated with worse disease-free and overall survival. In the multivariate analysis, low miR-34c-5p expression was associated with an increased risk of recurrence.
  
  
  CONCLUSIONS
  A downregulation of miR-34c-5p in LSCC is independently associated with unfavorable disease-free survival, suggesting that miR-34c-5p might be a promising marker for evaluating the risk of recurrences.
  
  
  METHODS
  NA.
authors.affiliation: Department of Otorhinolaryngology, Umberto I University General Hospital, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Re
authors.affiliation: Department of Molecular and Clinical Sciences, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Artan
authors.initials: A
authors.lastname: Çeka
authors.affiliation: Pathologic Anatomy and Histopathology Division, Department of Biomedical Sciences and Public Health, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Corrado
authors.initials: C
authors.lastname: Rubini
authors.affiliation: Department of Biomedical Sciences and Public Health, Section of Medical Statistics, Faculty of Medicine, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Ferrante
authors.affiliation: Pathologic Anatomy and Histopathology Division, Department of Biomedical Sciences and Public Health, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Zizzi
authors.affiliation: Department of Otorhinolaryngology, Umberto I University General Hospital, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Federico M
authors.initials: FM
authors.lastname: Gioacchini
authors.affiliation: Department of Otorhinolaryngology, Umberto I University General Hospital, Polytechnic University of the Marches, Ancona, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Tulli
authors.affiliation: Center of Biostatistics, National Institute of Repose and Cure for Elderly-Scientific Institute for Hospitalization and Treatment (INRCA-IRCCS) Italian National Institute, Ancona, Italy.
authors.forename: Liana
authors.initials: L
authors.lastname: Spazzafumo
authors.affiliation: Department of Otorhinolaryngology, University of Pisa, Pisa, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Sellari-Franceschini
authors.affiliation: Department of Molecular and Clinical Sciences, Polytechnic University of the Marches, Ancona, Italy.|Center of Clinical Pathology and Innovative Therapy, INRCA-IRCCS Italian National Institute, Ancona, Italy.
authors.forename: Antonio D
authors.initials: AD
authors.lastname: Procopio
authors.affiliation: Department of Molecular and Clinical Sciences, Polytechnic University of the Marches, Ancona, Italy.|Center of Clinical Pathology and Innovative Therapy, INRCA-IRCCS Italian National Institute, Ancona, Italy.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Olivieri
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007558:Italy / Q000453:epidemiology; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D012334:RNA, Neoplasm / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm
keywords: Laryngeal squamous cell carcinoma; biomarkers; miR-34c-5p; microRNA; prognosis; recurrence