title: A Dual Electrochemical Sensor Based on a Test-strip Assay for the Quantitative Determination of Albumin and Creatinine.
abstract: A dual-electrochemical sensor based on a test-strip assay with immunochemistry and enzyme reactions has been developed for the determination of albumin and creatinine. Each nitrocellulose membrane with an immobilization area of an anti-albumin antibody or three enzymes was prepared in the device with three working electrodes for measuring albumin, creatinine, and ascorbic acid, as well as an Ag/AgCl electrode used as a counter/pseudo-reference electrode. The reactions of three enzymes were initiated by flowing a solution containing creatinine to detect an oxidation current of hydrogen peroxide. A sandwich-type immunocomplex was formed by albumin and antibody labeled with glucose oxidase (GOx). Captured GOx catalyzed the reduction of Fe(CN)6(3-) to Fe(CN)6(4-), which was oxidized electrochemically to determine the captured albumin. The responses for creatinine and albumin increased with the concentrations in millimolar order and over the range 18.75 - 150 μg mL(-1), respectively. The present sensor would be a distinct demonstration for producing quantitative dual-assays for various biomolecules used for clinical diagnoses.
authors.affiliation: University of Hyogo.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasukawa
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kiba
authors.forename: Fumio
authors.initials: F
authors.lastname: Mizutani
mesh_terms: D000418:Albumins / Q000032:analysis* / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002846:Chromatography, Affinity; D003101:Collodion / Q000737:chemistry; D003404:Creatinine / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005292:Ferricyanides / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057446:Hydrodynamics; D011934:Reagent Strips / Q000737:chemistry*
chemical_list: D000418:Albumins; D055910:Antibodies, Immobilized; D004800:Enzymes, Immobilized; D005292:Ferricyanides; D011934:Reagent Strips; C007931:hexacyanoferrate III; D003101:Collodion; D003404:Creatinine; D005949:Glucose Oxidase