title: Antioxidative and Anticanceric Activities of Magnolia (Magnolia denudata) Flower Petal Extract Fermented by Pediococcus acidilactici KCCM 11614.
abstract: In this study, the effects of magnolia (Magnolia (M.) denudata) extract fermentation in increasing the extract's antioxidative and anticancer activities were investigated. Magnolia was fermented by Pediococcus acidilactici KCCM 11614. The total phenolic content was determined by the Folin-Ciocalteu's method and the antioxidative effects by 1,1-diphenyl-2-picrylhydrazy (DPPH) and ferric reducing ability of plasma (FRAP) assay. Anticancer activity against cancer and normal cells was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). Total phenolic content during fermentation increased from 38.1 to 47.0 mg gallic acid equivalent (GAE)/g of solid matter. The radical scavenging activity was 91.4% after 72 h fermentation. Fermented magnolia's antioxidative effect was threefold higher than that of the (non-fermented) control. Fermentation (48 h) increased anticanceric activity against AGS, LoVo, and MCF-7 cancer cells 1.29- to 1.36-fold compared with that of the control, but did not affect MRC-5 (normal) cells, suggesting that fermented magnolia could be used as a natural antioxidative and anticancer agent.
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea.
authors.forename: Hye
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea. zmzm852@naver.com.
authors.forename: Hyun-Suk
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea. insomnia@daum.net.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Eom
authors.affiliation: Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea. richard44@hanmail.net.
authors.forename: Kee-Tae
authors.initials: KT
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea. hdpaik@konkuk.ac.kr.|Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea. hdpaik@konkuk.ac.kr.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005285:Fermentation; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D031566:Magnolia / Q000737:chemistry*; D010376:Pediococcus / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010936:Plant Extracts
keywords: Magnolia denudate; Pediococcus acidilactici; anticanceric activity; antioxidative effect; flavonoid