title: Synchronisation of the follicular wave with GnRH and PGF2α analogue for a timed breeding programme in dromedary camels (Camelus dromedarius).
abstract: This study was conducted to develop a hormone protocol that precisely synchronises follicular development for a timed breeding (TB) programme in dromedary camels. To examine the effect of GnRH treatment at four known stages of follicular development, animals were treated with GnRH when the largest follicle of the wave was 4-7, 8-11, 12-17 and 18-27 mm in diameter. Transrectal ultrasonography was carried out daily up to 20 days after treatment. A hormone protocol (FWsynch) for the synchronisation of follicular wave and TB consisting of GnRH-1 (GnRH) on Day 0, PG-1 (PGF2α) on Day 7, GnRH-2 on Day 10 and PG-2 on Day 17 was initiated at four known stages of follicular development. Ovarian structures were monitored by ultrasonography. The FWsynch protocol was initiated at random stages of follicle development and animals were bred by natural mating at a fixed time at the research facility and in field. The pregnancy was diagnosed by ultrasonography. GnRH treatment in animals with a dominant follicle (DF) of ≥ 11 mm in diameter resulted in synchronous new follicular wave emergence, whereas in animals with a DF ≤ 10 mm, the treatment did not alter the development of the existing follicular wave. The FWsynch protocol was effective in synchronising the follicular wave for TB irrespective of the stage of follicular development at the beginning of the protocol. TB using FWsynch protocol resulted in a pregnancy rate of 60.2% in a research facility and 53.6% and 45.6% in normal and infertile camels respectively under field conditions.
authors.affiliation: Division of Reproduction, Laboratories and Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, P.O. Box 64, P.C. 111 Muscat, Oman. Electronic address: drmanjunathvet@gmail.com.
authors.forename: B M
authors.initials: BM
authors.lastname: Manjunatha
authors.affiliation: Division of Reproduction, Laboratories and Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, P.O. Box 64, P.C. 111 Muscat, Oman.
authors.forename: Samir
authors.initials: S
authors.lastname: Al-Bulushi
authors.affiliation: Division of Reproduction, Laboratories and Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, P.O. Box 64, P.C. 111 Muscat, Oman.
authors.forename: N
authors.initials: N
authors.lastname: Pratap
mesh_terms: D000818:Animals; D002162:Camelus / Q000502:physiology*; D015237:Dinoprost / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects*; D010060:Ovulation / Q000187:drug effects*; D011247:Pregnancy
chemical_list: D007987:Gonadotropin-Releasing Hormone; D015237:Dinoprost
keywords: Dromedary camel; GnRH; Synchronisation of follicular wave; Timed breeding