title: Bioassay-guided isolation and POM analyses of a new immunomodulatory polyphenolic constituent from Callistemon viridiflorus.
abstract: Chromatographic separation of 80% EtOH extract of Callistemon viridiflorus leaves led to the isolation of six known constituents (1-6) along with a new polyphenolic compound 7 identified as apigenin 4'-O-β-d-glucopyranosyl-(1″' → 4″)-O-β-d-glucopyranoside. The ethanolic extract of C. viridiflorus leaves and isolated compounds were evaluated for in vitro immunomodulatory activity by means of RAW 264.7 macrophages proliferation (MTT) assay. Ethanolic extract of leaves and compounds 1, 3, 4, 6 and 7 caused a significant increase in macrophage proliferation; these findings may suggest that this medicinal plant could be utilised as an excellent source of compounds for immunomodulatory activity.
authors.affiliation: a Department of Pharmacognosy, Faculty of Pharmacy , Umm Al-Qura University , Makkah 21955 , Saudi Arabia.|b Department of Pharmacognosy, Faculty of Pharmacy , Helwan University , Cairo 11795 , Egypt.
authors.forename: Mohamed I S
authors.initials: MI
authors.lastname: Abdelhady
authors.affiliation: b Department of Pharmacognosy, Faculty of Pharmacy , Helwan University , Cairo 11795 , Egypt.
authors.forename: Amel M
authors.initials: AM
authors.lastname: Kamal
authors.affiliation: c Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.affiliation: d Department of Chemistry , The University of Jordan , Amman 11942 , Jordan.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Mubarak
authors.affiliation: e Department of Chemistry, Materials Chemistry Laboratory , Mohammed First University, Faculty of Sciences , Oujda 60000 , Morocco.
authors.forename: Taibi Ben
authors.initials: TB
authors.lastname: Hadda
mesh_terms: D000818:Animals; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D000067996:RAW 264.7 Cells
chemical_list: D005960:Glucosides; D007155:Immunologic Factors; D010936:Plant Extracts; C000611768:apigenin 4'-O-beta-D-glucopyranosyl-(1''-4')-O-beta-D-glucopyranoside; D047310:Apigenin
keywords: Callistemon viridiflorus; POM analyses; flavonoids; immunomodulatory