title: Imaging stress.
abstract: Recent innovations in cell biology and imaging approaches are changing the way we study cellular stress, protein misfolding, and aggregation. Studies have begun to show that stress responses are even more variegated and dynamic than previously thought, encompassing nano-scale reorganization of cytosolic machinery that occurs almost instantaneously, much faster than transcriptional responses. Moreover, protein and mRNA quality control is often organized into highly dynamic macromolecular assemblies, or dynamic droplets, which could easily be mistaken for dysfunctional "aggregates," but which are, in fact, regulated functional compartments. The nano-scale architecture of stress-response ranges from diffraction-limited structures like stress granules, P-bodies, and stress foci to slightly larger quality control inclusions like juxta nuclear quality control compartment (JUNQ) and insoluble protein deposit compartment (IPOD), as well as others. Examining the biochemical and physical properties of these dynamic structures necessitates live cell imaging at high spatial and temporal resolution, and techniques to make quantitative measurements with respect to movement, localization, and mobility. Hence, it is important to note some of the most recent observations, while casting an eye towards new imaging approaches that offer the possibility of collecting entirely new kinds of data from living cells.
authors.affiliation: Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, 91904, Israel.|Alexander Grass Center for Bioengineering, Hebrew University of Jerusalem, Jerusalem, Israel, 91904.
authors.forename: Shlomi
authors.initials: S
authors.lastname: Brielle
authors.affiliation: Department of Physics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Rotem
authors.initials: R
authors.lastname: Gura
authors.affiliation: Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, 91904, Israel. dan@cc.huji.ac.il.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kaganovich
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods*; D002479:Inclusion Bodies / Q000378:metabolism; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*
keywords: Aggregation; Chaperone; Dynamic droplets; FLIP; FRAP; IPOD; Inclusion; JUNQ; Misfolded protein; P-bodies; PhoC; Stress foci; Stress granules