title: [Comparison for ELISA and CLIA of serum 25-hydroxy vitamin D determination].
abstract: OBJECTIVE
  To estimate the bias between the enzyme linked immunosorbent assay (ELISA) and chemiluminescence immunoassay (CLIA) of serum 25-hydroxy vitamin D (25 (OH) D) determination, and to evaluate the correspondence of two methods.
  
  
  METHODS
  Forty human serum samples were collected, and the serum 25 (OH) D were determined by immunodiagnostic systems 25-hydroxy vitamin D EIA and immunodiagnostic systems iSYS analyser. According to the standards of NCCLS EP9-A2 files, the within and without outlier, test adequate range, used linear regression procedure, and compare average and maximum bias between these two methods were excluded.
  
  
  RESULTS
  In the linear regression analysis, the results of the two methods were correlated, y = 0.995x + 2.3928, r = 0.981. The relative standard deviation between these two methods was smaller with the higher serum 25(OH) D levels.
  
  
  CONCLUSIONS
  The correlation and repeatability of serum 25 (OH) D determination by ELISA and CLIA are fine.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Yun
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Piao
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
mesh_terms: D002112:Calcifediol; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D011237:Predictive Value of Tests; D012015:Reference Standards; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D; D002112:Calcifediol