title: Determination of galangin in rat plasma by UPLC and pharmacokinetic study.
abstract: In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of galangin in rat plasma using diazepam as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm particle size) and ultraviolet detection set at a wavelength of 360nm. The method was linear over the concentration range 10-1000ng/mL with a lower limit of quantification (LLOQ) of 10ng/mL. Inter- and intra-day precision (RSD %) were all within 9.5% and the accuracy (RE %) was equal or lower than 8.9%. Recoveries of galangin and IS were more than 78.3%. Stability studies showed that galangin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of galangin to rats.
authors.affiliation: Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of Orthopedics, Luoyang 471002, China.
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Feng
authors.affiliation: Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of Orthopedics, Luoyang 471002, China. Electronic address: hnzhanghh@126.com.
authors.forename: Hang-hang
authors.initials: HH
authors.lastname: Zhang
authors.affiliation: Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of Orthopedics, Luoyang 471002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Jun-long
authors.initials: JL
authors.lastname: Niu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D059625:Liquid-Liquid Extraction; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005419:Flavonoids; C037032:galangin
keywords: Galangin; Pharmacokinetic; Rat plasma; UPLC