title: Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model.
abstract: The aim of this study was to investigate the possible therapeutic effects of Doxycycline (Dox) on endometriotic lesions in an experimental rat model. Thirty-seven female Wistar albino rats with surgically induced endometriosis were randomized and divided into four groups. The rats were administered 5 mg/kg/day oral Dox in Group 1 (low-dose Dox group, n=9), 20 mg/kg/day oral Dox in Group 2 (high-dose Dox group, n=10) and 1 mg/kg single dose, subcutaneous leuprolide acetate in Group 3 (leuprolide acetate group, n=9). The rats in Group 4 (control group, n=9) were given no medication. The rats received medication for three weeks and were then sacrificed to evaluate the morphological and histological features of the implants. Matrix metalloproteinase (MMP)-9 immunoreactivity of the implants was also evaluated. The size of the endometriotic implants decreased in Groups 1-3 but statistically significant differences were not observed among the groups. The mean surface area of the endometriotic implants decreased from 69.3±30.8 to 52.1±27.0 mm² in Group 1 (P>0.05), from 60.2±18.9 to 38.6±28.7 mm² in Group 2 (P>0.05) and from 58.1±33.1 to 26±9.0 mm² in Group 3 (P=0.03). The epithelial MMP-9 immunohistochemical score was significantly higher in Group 1 and lower in Group 3 when compared with the control group (Group 4) (P=0.042 and P=0.014, respectively). When the stromal MMP-9 immunohistochemical and histopathological scores of the endometriotic implants were compared, no statistically significant differences were found among the groups. Although there was no statistically significant difference, Dox reduced the endometriotic implant area in the rat endometriosis model. Further studies are required to investigate the potential efficacy of Dox in endometriosis due to its widespread use and tolerability.
authors.affiliation: Department of Obstetrics and Gynecology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Umit
authors.initials: U
authors.lastname: Goktolga
authors.affiliation: Department of Obstetrics and Gynecology, Zekai Tahir Burak Women's Health Education and Research Hospital, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Sabri
authors.initials: S
authors.lastname: Cavkaytar
authors.affiliation: Department of Obstetrics and Gynecology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Sadiman Kiykac
authors.initials: SK
authors.lastname: Altinbas
authors.affiliation: Department of Obstetrics and Gynecology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Omer Lutfi
authors.initials: OL
authors.lastname: Tapisiz
authors.affiliation: Department of Pediatric Infectious Diseases, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Anil
authors.initials: A
authors.lastname: Tapisiz
authors.affiliation: Department of Pathology, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Erdem
keywords: Doxycycline; endometriosis; leuprolide acetate; matrix metalloproteinase 9; rat