title: Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model.
abstract: Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15 J/cm(2) . Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10 J/cm(2) reached wound closure after 72 h, followed by 15 J/cm(2) after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15 J/cm(2) maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.
authors.affiliation: Rizzoli Orthopaedic Institute, Laboratory of Preclinical and Surgical Studies, via di Barbiano 1/10, Bologna 40136, Italy.
authors.forename: Matilde
authors.initials: M
authors.lastname: Tschon
authors.affiliation: University of Bologna, Unit of Orthodontics, Department of Biomedical and Neuromotor Sciences, via San Vitale 59, Bologna 40125, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Incerti-Parenti
authors.affiliation: Rizzoli Orthopaedic Institute, Laboratory of Preclinical and Surgical Studies, via di Barbiano 1/10, Bologna 40136, ItalycUniversity of Bologna, Department of Medical and Surgical Sciences, via Massarenti 9, Bologna 40138, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Cepollaro
authors.affiliation: University of Bologna, Unit of Periodontology, Department of Biomedical and Neuromotor Sciences, via San Vitale 59, Bologna 40125, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Checchi
authors.affiliation: Rizzoli Orthopaedic Institute, Laboratory of Preclinical and Surgical Studies, via di Barbiano 1/10, Bologna 40136, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D010006:Osteoblasts / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects*