title: Identification of rice blast resistance genes in the elite hybrid rice restorer line Yahui2115.
abstract: Rice blast, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most serious rice diseases worldwide. We previously developed an elite hybrid rice restorer line with high resistance to rice blast, Yahui2115 (YH2115). To identify the blast resistance genes in YH2115, we first performed expression profiling on previously reported blast resistance genes and disease assay on monogenic lines, and we found that Pi2, Pi9, and Pikm were the most likely resistance candidates in YH2115. Furthermore, RNA interference and linkage analysis demonstrated that silencing of Pi2 reduced the blast resistance of YH2115 and a Pi2 linkage marker was closely associated with blast resistance in an F2 population generated from YH2115. These data suggest that the broad-spectrum blast resistance gene Pi2 contributes greatly to the blast resistance of YH2115. Thus, YH2115 could be used as a new germplasm to facilitate rice blast resistance breeding in hybrid rice breeding programs.
authors.affiliation: a Department of Plant Pathology, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.|b Mianyang Academy of Agricultural Sciences, Mianyang, Sichuan 621023, China.|c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.affiliation: a Department of Plant Pathology, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Deqiang
authors.initials: D
authors.lastname: Li
authors.affiliation: c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Department of Plant Pathology, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.|e Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences / Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Deyang 618000, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Guo
authors.affiliation: a Department of Plant Pathology, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yiwan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yuangen
authors.initials: Y
authors.lastname: Lu
authors.affiliation: a Department of Plant Pathology, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yongju
authors.initials: Y
authors.lastname: Xu
authors.affiliation: c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.affiliation: a Department of Plant Pathology, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Huang
authors.affiliation: c Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.|d Provincial Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Wang
mesh_terms: D002678:Chimera; D032461:Chromosomes, Plant; D060467:Disease Resistance; D020869:Gene Expression Profiling; D017343:Genes, Plant; D008040:Genetic Linkage; D012275:Oryza / Q000235:genetics*; D000069600:Plant Breeding; D034622:RNA Interference
keywords: blast resistance; hybrid rice; lignée monogénique; lignée restauratrice; monogenic line; restorer line; riz hybride; résistance à la pyriculariose