title: Short-chain polyisoprenoids in the yeast Saccharomyces cerevisiae - New companions of the old guys.
abstract: Dolichols are, among others, obligatory cofactors of protein glycosylation in eukaryotic cells. It is well known that yeast cells accumulate a family of dolichols with Dol-15/16 dominating while upon certain physiological conditions a second family with Dol-21 dominating is noted. In this report we identified the presence of additional short-chain length polyprenols - all-trans Pren-7 in three yeast strains (SS328, BY4741 and L5366), Pren-7 was accompanied by traces of putative Pren-6 and -8. Moreover, in two of these strains a single polyprenol mainly-cis-Pren-11 was synthesized at the stationary phase of growth. Identity of polyprenols was confirmed by HR-HPLC/MS, NMR and metabolic labeling. Additionally, simvastatin inhibited their biosynthesis.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland. Electronic address: surmacz@ibb.waw.pl.
authors.forename: Liliana
authors.initials: L
authors.lastname: Surmacz
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Wojcik
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kania
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16, S-106 91 Stockholm, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Bentinger
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Danikiewicz
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16, S-106 91 Stockholm, Sweden.
authors.forename: Gustav
authors.initials: G
authors.lastname: Dallner
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Surowiecki
authors.affiliation: Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Cmoch
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Swiezewska
mesh_terms: D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*
chemical_list: D013729:Terpenes
keywords: Dolichol; Polyisoprenoid alcohols; Prenol; Saccharomyces cerevisiae; yeast