title: Alpha-synuclein immunoreactivity patterns in the enteric nervous system.
abstract: We aimed to compare immunoreactivity patterns of four different anti-α-syn antibodies in surgical specimens of the gastrointestinal tract of Parkinson disease and control cases. Surgical specimens from stomach, small and large bowel of 6 PD cases and 12 controls were studied. Primary antibodies: anti-α-syn clone KM51, anti-phosphorylated α-syn Ser129, anti-α-syn clone 15G7 and anti-nitrated α-syn505. We found different immunoreactivity patterns: (a) coarse, Lewy-body-like aggregates labelled by the 4 antibodies and detected in 4/6 PD cases and in 1/12 controls; (b) distinct punctate cytoplasmic staining of ganglion cells labelled by anti-phosphorylated-α-syn and detected in 3/6 PD cases and 3/12 controls; (c) fine diffuse, synaptic-type staining of neural structures labelled by anti-α-syn-15G7 and anti-nitrated-α-syn505 and detected in all subjects. We conclude that different specific and non-specific immunoreactivity patterns are detected in surgical specimens of gastrointestinal tract when using different anti-α-syn antibodies, as they recognize different epitopes and states of alpha-synuclein protein. Coarse aggregates in neural structures seem to be the most promising marker for the diagnosis of Lewy-body parkinsonism when evaluating abnormal α-syn in the gastrointestinal tract.
authors.affiliation: Department of Pathology, Barcelona, Spain.
authors.forename: Iban
authors.initials: I
authors.lastname: Aldecoa
authors.affiliation: Parkinson's Disease and Movement Disorders Unit Neurology Service Institut de Neurociències Hospital Clínic, University of Barcelona, Barcelona, Spain; Institut d'Investigacions Biomèdiques August Pi I Sunyer-IDIBAPS, Barcelona, Spain.
authors.forename: Judith
authors.initials: J
authors.lastname: Navarro-Otano
authors.affiliation: Department of Neurology, Medical University of Innsbruck, Austria.
authors.forename: Nadia
authors.initials: N
authors.lastname: Stefanova
authors.affiliation: Department of Neurology, Medical University of Innsbruck, Austria.
authors.forename: Fabienne S
authors.initials: FS
authors.lastname: Sprenger
authors.affiliation: Department of Neurology, Medical University of Innsbruck, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Seppi
authors.affiliation: Department of Neurology, Medical University of Innsbruck, Austria.
authors.forename: Werner
authors.initials: W
authors.lastname: Poewe
authors.affiliation: Department of Pathology, Barcelona, Spain; Tumour Bank of the Biobank, Hospital Clinic, IDIBAPS, Barcelona, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Cuatrecasas
authors.affiliation: Parkinson's Disease and Movement Disorders Unit Neurology Service Institut de Neurociències Hospital Clínic, University of Barcelona, Barcelona, Spain; Institut d'Investigacions Biomèdiques August Pi I Sunyer-IDIBAPS, Barcelona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Valldeoriola
authors.affiliation: Neurological Tissue Bank of the Biobank, Hospital Clinic, IDIBAPS, Barcelona, Spain.
authors.forename: Ellen
authors.initials: E
authors.lastname: Gelpi
authors.affiliation: Parkinson's Disease and Movement Disorders Unit Neurology Service Institut de Neurociències Hospital Clínic, University of Barcelona, Barcelona, Spain; Institut d'Investigacions Biomèdiques August Pi I Sunyer-IDIBAPS, Barcelona, Spain; Centro en Red para la Investigación de Enfermedades Neurodegenerativas CIBERNED, Spain. Electronic address: etolosa@clinic.ub.es.
authors.forename: Eduard
authors.initials: E
authors.lastname: Tolosa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000378:metabolism / Q000601:surgery; D017615:Enteric Nervous System / Q000378:metabolism*; D005260:Female; D041981:Gastrointestinal Tract / Q000294:innervation / Q000378:metabolism / Q000601:surgery; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism / Q000601:surgery; D016631:Lewy Bodies / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009197:Myenteric Plexus / Q000378:metabolism; D010300:Parkinson Disease / Q000378:metabolism* / Q000601:surgery; D066329:Protein Aggregates; D055815:Young Adult; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D066329:Protein Aggregates; D051844:alpha-Synuclein
keywords: Alpha-synuclein; Enteric nervous system; Gastrointestinal tract; Lewy pathology; Parkinson disease