title: A Computational Approach for Designing a Universal Epitope-Based Peptide Vaccine Against Nipah Virus.
abstract: Nipah virus (NiV) is highly pathogenic single-stranded negative sense RNA virus. It can cause severe encephalitis and respiratory disease in humans. In addition, NiV infects a large range of host including mammals. As a result of its higher zoonotic potential and pathogenicity for human, it has been rated as an alert in recent days. A therapeutic treatment or vaccines has become elusive to fight against this virus. In this study, the attachment (G) and fusion (F) glycoproteins of NiV, responsible for the viral attachment and entry to the host cell, were selected to develop epitope-based vaccine against Nipah virus. Epitopes were identified from the conserved region of G and F protein of NiV. Both B-cell and T-cell immunity were checked to affirm it that these epitopes will be able to induce humoral and cellular immunity. A total of 6 T-cell epitopes and 19 significant HLA-epitope interactions were identified. Eventually it has shown an acceptable percentage in population coverage (46.45 %) and efficient binding with HLA molecule by molecular docking study.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
authors.forename: Mohammad Tuhin
authors.initials: MT
authors.lastname: Ali
authors.forename: Mohammed Monzur
authors.initials: MM
authors.lastname: Morshed
authors.forename: Faizule
authors.initials: F
authors.lastname: Hassan
mesh_terms: D017076:Computer-Aided Design; D015195:Drug Design; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D045464:Henipavirus Infections / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D000071497:Immunogenicity, Vaccine; D008024:Ligands; D018448:Models, Immunological; D062105:Molecular Docking Simulation; D045405:Nipah Virus / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D006680:HLA Antigens; D008024:Ligands; D011993:Recombinant Fusion Proteins; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014765:Viral Vaccines