title: Cultured Cells from the Human Oocyte Cumulus Niche Are Efficient Feeders to Propagate Pluripotent Stem Cells.
abstract: Pluripotency is at the crossroads of stem cell research and biology of reproduction. The mature metaphase II oocyte contains the key factors for pluripotency induction and maintenance as assessed by its capacity to reprogram somatic nuclei. The cumulus cells (CCs) niche that surrounds the oocyte is crucial for its maturation and presumably for the oocyte to acquire its competence to confer pluripotency. In this study, we examined whether cells cultured from the human mature metaphase II oocyte CC niche (hCC) could be used as feeders for the propagation of human induced pluripotent stem cells. The induced pluripotent (iPS) cells cultured on hCC (hCC-iPS) were assessed for their pluripotency potential by their expression of pluripotency-associated genes such as Oct4, Nanog, and TRA1-60 and their competence to differentiate into the three germ layers in vitro (embryoid bodies) as well as in vivo (teratoma formation). We show that not only the hCC-iPS cells maintained their pluripotency potential, but they also exhibited much better self-renewal performance in terms of proliferation rate compared to the same cells cultured on human foreskin fibroblast (hFF) feeders (hFF-iPS). A comparative gene expression profile study of hCC and hFF revealed significant differences (P < 0.05) in expression of cellular matrix components and an upregulation in hCC of genes known to be important players in cell proliferation such as interleukin 6 gene (IL6).
authors.affiliation: 1 Université Montpellier , UFR de Médecine, Montpellier, France .|2 Institute for Regenerative Medicine and Biotherapy, INSERM U1203, CHRU Montpellier, Hôpital Saint-Eloi , Montpellier, France .
authors.forename: Said
authors.initials: S
authors.lastname: Assou
authors.affiliation: 2 Institute for Regenerative Medicine and Biotherapy, INSERM U1203, CHRU Montpellier, Hôpital Saint-Eloi , Montpellier, France .
authors.forename: Emilie
authors.initials: E
authors.lastname: Pourret
authors.affiliation: 3 Institut des Neurosciences de Montpellier, INSERM U1051, Hôpital Saint-Eloi , Montpellier, France .
authors.forename: Marie
authors.initials: M
authors.lastname: Péquignot
authors.affiliation: 4 Pathology Department, University Hospital Gui de Chauliac , Montpellier, France .
authors.forename: Valérie
authors.initials: V
authors.lastname: Rigau
authors.affiliation: 3 Institut des Neurosciences de Montpellier, INSERM U1051, Hôpital Saint-Eloi , Montpellier, France .
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Kalatzis
authors.affiliation: 2 Institute for Regenerative Medicine and Biotherapy, INSERM U1203, CHRU Montpellier, Hôpital Saint-Eloi , Montpellier, France .
authors.forename: Ounissa
authors.initials: O
authors.lastname: Aït-Ahmed
authors.affiliation: 1 Université Montpellier , UFR de Médecine, Montpellier, France .|2 Institute for Regenerative Medicine and Biotherapy, INSERM U1203, CHRU Montpellier, Hôpital Saint-Eloi , Montpellier, France .|5 ART-PGD Department, CHU Montpellier, Hôpital Arnaud de Villeneuve , Montpellier, France .
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D000071858:Epithelial Cell Adhesion Molecule; D061252:Feeder Cells / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D009865:Oocytes / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013724:Teratoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014183:Transplantation, Heterologous; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D014746:Vimentin