title: Biohydrogen production by co-fermentation of crude glycerol and apple pomace hydrolysate using co-culture of Enterobacter aerogenes and Clostridium butyricum.
abstract: Co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production. This study evaluated potential of apple pomace hydrolysate (APH) co-fermented with crude glycerol (CG) for increased H2 production and decreased by-products formation. The central composite design (CCD) along with response surface methodology (RSM) was used as tool for optimization and 15 g/L of CG, 5 g/L of APH and 15% (v/v) inoculum were found to be optimum to produce as high as 26.07 ± 1.57 mmol H2/L of medium. The p-value of 0.0017 indicated that APH at lower concentration had a significant effect on H2 production. By using CG as sole carbon source, reductive pathway of glycerol metabolism was favored with 19.46 mmol H2/L. However, with APH, oxidative pathway was favored with higher H2 production (26.07 ± 1.57 mmol/L) and decrease in reduced by-products (1,3-propanediol and ethanol) formation. APH inclusion enhanced H2 production, and decreased substrate inhibition.
authors.affiliation: Institut National de la Recherche Scientifique, Centre - Eau Terre Environnement, 490, Rue de la Couronne, Québec, QC G1K 9A9, Canada.
authors.forename: Vinayak Laxman
authors.initials: VL
authors.lastname: Pachapur
authors.affiliation: Institut National de la Recherche Scientifique, Centre - Eau Terre Environnement, 490, Rue de la Couronne, Québec, QC G1K 9A9, Canada.
authors.forename: Saurabh Jyoti
authors.initials: SJ
authors.lastname: Sarma
authors.affiliation: Institut National de la Recherche Scientifique, Centre - Eau Terre Environnement, 490, Rue de la Couronne, Québec, QC G1K 9A9, Canada. Electronic address: satinder.brar@ete.inrs.ca.
authors.forename: Satinder Kaur
authors.initials: SK
authors.lastname: Brar
authors.affiliation: Centre de Recherche Industrielle du Québec (CRIQ), Québec, QC, Canada.
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Bihan
authors.affiliation: Centre de Recherche Industrielle du Québec (CRIQ), Québec, QC, Canada.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Buelna
authors.affiliation: CO(2) Solutions Inc., 2300, Rue Jean-Perrin, Québec, QC G2C 1T9, Canada.
authors.forename: Mausam
authors.initials: M
authors.lastname: Verma
mesh_terms: D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D046972:Clostridium butyricum / Q000378:metabolism*; D018920:Coculture Techniques / Q000379:methods; D021902:Enterobacter aerogenes / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D005990:Glycerol / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D027845:Malus / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism
chemical_list: D056804:Biofuels; D011409:Propylene Glycols; D000431:Ethanol; C041787:1,3-propanediol; D002244:Carbon; D006859:Hydrogen; D005990:Glycerol
keywords: Apple pomace hydrolysate; Biohydrogen; Co-culture; Co-substrate; Crude glycerol