title: A Significant Upregulation of miR-886-5p in High Grade and Invasive Bladder Tumors.
abstract: To investigate the expression alteration of  miR-886-5p in bladder tumors and evaluating its expression level as a potential biomarker in this type of cancer.
  
  
  
  Formalin-fixed paraffin-embedded (FFPE) samples of bladder tumors belonging to 70 patients whom had been referred to the Shahid Labbafi-Nejad medical center were obtained from the archival collection of pathology department. After RNA extraction and cDNA synthesis, expression levels of  miR-886-5p were quantified by a real-time reverse transcription polymerase chain reaction (RT-PCR) approach.
  
  
  
  Our data revealed a significant upregulation (~3 times) of  miR-886-5p in high grade bladder tumors, compared to the low grade ones (P < .05). Moreover, its expression level could significantly discriminate noninvasive (Ta, T1) from invasive (T-2T4) tumor stages.
  
  
  
  Our data suggests a potential role for  miR-886-5p in progression of bladder cancer.
authors.affiliation: Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Khoshnevisan
authors.affiliation: Department of Pathology, Labbafi-Nejad Medical Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Parvin
authors.affiliation: Department of Anatomy, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Ghorbanmehr
authors.affiliation: Department of Pathology, Labbafi-Nejad Medical Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Hatefi
authors.affiliation: Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Galehdari
authors.affiliation: Urology and Nephrology Research Center, Labbafi-Nejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. samziaee@gmail.com.
authors.forename: Seyed Amir Mohsen
authors.initials: SA
authors.lastname: Ziaee
authors.affiliation: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. sjmowla@modares.ac.ir.
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012334:RNA, Neoplasm / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C559060:MIRN886 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm