title: Enhanced lignocellulosic biomass hydrolysis by oxidative lytic polysaccharide monooxygenases (LPMOs) GH61 from Gloeophyllum trabeum.
abstract: Lignocellulose is a renewable resource that is extremely abundant, and the complete enzymatic hydrolysis of lignocellulose requires a cocktail containing a variety of enzyme groups that act synergistically. The hydrolysis efficiency can be improved by introducing glycoside hydrolase 61 (GH61), a new enzyme that belongs to the auxiliary activity family 9 (AA9). GH61was isolated from Gloeophyllum trabeum and cleaves the glycosidic bonds on the cellulose surface via oxidation of various carbons. In this study, we investigated the properties of GH61. GtGH61 alone did not exhibit any notable activity, but the synergistic activity of GtGH61 with xylanase (GtXyl10G) or cellulase (GtCel5B) showed efficient bioconversion rates of 56 and 174% in pretreated kenaf (Hibiscus cannabinus L.) and oak (Quercus spp.), respectively. Furthermore, the GtGH61 activity was strongly accelerated in the presence of cobalt Co(2+). Enzyme cocktails (GtXyl10G, GtCel5B, and GtGH61) increased the amount of sugar released by 7 and 6% for pretreated oak and kenaf, respectively, and the addition of Co(2+) stimulated bioconversion by 12 and 11% in pretreated oak and kenaf, respectively.
authors.affiliation: Department of Wood Science and Landscape Architechture, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Sera
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Younho
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Ho Myeong
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Wood Science and Landscape Architechture, Chonnam National University, Gwangju 500-757, Republic of Korea; Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: baehj@chonnam.ac.kr.
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D056804:Biofuels; D018533:Biomass; D062610:Fungal Polysaccharides / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D006868:Hydrolysis; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D056804:Biofuels; D062610:Fungal Polysaccharides; D005656:Fungal Proteins; C036909:lignocellulose; D008031:Lignin; D006899:Mixed Function Oxygenases
keywords: AA9; Bioethanol; GH61; Lignocellulose bioconversion; Lytic polysaccharide monooxygenase