title: Monitoring Tumor Targeting and Treatment Effects of IRDye 800CW and GX1-Conjugated Polylactic Acid Nanoparticles Encapsulating Endostar on Glioma by Optical Molecular Imaging.
abstract: Molecular imaging used in cancer diagnosis and therapeutic response monitoring is important for glioblastoma (GBM) research. Antiangiogenic therapy currently is one of the emerging approaches for GBM treatment. In this study, a multifunctional nanoparticle was fabricated that can facilitate the fluorescence imaging of tumor and deliver a therapeutic agent to the tumor region in vivo and therefore possesses broad application in cancer diagnosis and treatment. This particle was polylactic acid (PLA) nanoparticles encapsulating Endostar, which was further conjugated with GX1 peptide and the near-infrared (NIR) dye IRDye 800CW (IGPNE). We demonstrated noninvasive angiogenesis targeting and therapy of IGPNE on U87MG xenografts in vivo using dual-modality optical molecular imaging including NIR fluorescence molecular imaging (FMI) and bioluminescence imaging (BLI). The NIR FMI results demonstrated that IGPNE had more accumulation to the tumor site compared to free IRDye 800CW. To further evaluate the antitumor treatment efficacy of IGPNE, BLI and immunohistochemistry analysis were performed on tumor-bearing mice. With the aid of molecular imaging, the results confirmed that IGPNE enhanced antitumor treatment efficacy compared to free Endostar. In conclusion, IGPNE realizes real-time imaging of U87MG tumors and improves the antiangiogenic therapeutic efficacy in vivo.
authors.forename: Yaqian
authors.initials: Y
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Du
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Lijia
authors.initials: L
authors.lastname: Jing
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liang
authors.forename: Chongwei
authors.initials: C
authors.lastname: Chi
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Dai
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D001557:Benzenesulfonates / Q000627:therapeutic use*; D001835:Body Weight; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D043169:Endostatins / Q000627:therapeutic use*; D005453:Fluorescence; D005910:Glioma / Q000098:blood supply / Q000188:drug therapy*; D006801:Humans; D007211:Indoles / Q000627:therapeutic use*; D019344:Lactic Acid / Q000737:chemistry; D008163:Luminescent Measurements; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D061848:Optical Imaging; D010455:Peptides / Q000627:therapeutic use*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D011994:Recombinant Proteins; D019265:Spectroscopy, Near-Infrared; D014018:Tissue Distribution; D016896:Treatment Outcome; D047368:Tumor Burden
chemical_list: D001557:Benzenesulfonates; D043169:Endostatins; C562366:IRDye 800CW; D007211:Indoles; D010455:Peptides; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D019344:Lactic Acid; C033616:poly(lactide); C522911:endostar protein