title: Comparative study of antibody immobilization mediated by lipid and polymer fibers.
abstract: Antibody immobilization and function retention are important to a variety of applications, including proteomics, drug discovery, diagnostics, and biosensors. The present study investigates antibody immobilization mediated by cholesteryl succinyl silane (CSS) fibers, in comparison to hydrophobic polycaprolactone (PCL) fibers and hydrophilic plasma-treated PCL fibers. When incubated with a model protein, the formation of protein aggregates is observed on hydrophobic PCL fibers but not on the more hydrophobic CSS fibers, indicating that CSS fibers immobilize proteins through mechanisms other than hydrophobic interaction. When exposed to a limited amount of antibody, CSS fibers immobilize more antibodies than plasma-treated PCL fibers and no fewer antibodies than PCL fibers. The function retention of antibodies immobilized on the fibers is analyzed using a cell-capture assay, which shows that the antibody-functionalized CSS fibrous matrices capture 6- or 7-fold more cells than the antibody-functionalized PCL or plasma-treated PCL fibrous matrices, respectively. Data collected from the study show that the lipid fiber-mediated immobilization of antibody not only maintains the advantages of physical immobilization such as easiness and rapidness of operation but also improves function retention.
authors.affiliation: Biomedical Engineering GIDP, University of Arizona, Thomas W. Keating Bioresearch Building, 1657 E Helen Street, Tucson, AZ 85721, USA.
authors.forename: Celine
authors.initials: C
authors.lastname: Cohn
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Arizona, 1130N Mountain Ave, Tucson, AZ 85721, USA.
authors.forename: Siu Ling
authors.initials: SL
authors.lastname: Leung
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Arizona, 1130N Mountain Ave, Tucson, AZ 85721, USA.
authors.forename: Zhengbao
authors.initials: Z
authors.lastname: Zha
authors.affiliation: Biomedical Engineering GIDP, University of Arizona, Thomas W. Keating Bioresearch Building, 1657 E Helen Street, Tucson, AZ 85721, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Crosby
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Arizona, 1130N Mountain Ave, Tucson, AZ 85721, USA.
authors.forename: Weibing
authors.initials: W
authors.lastname: Teng
authors.affiliation: Biomedical Engineering GIDP, University of Arizona, Thomas W. Keating Bioresearch Building, 1657 E Helen Street, Tucson, AZ 85721, USA; Department of Aerospace and Mechanical Engineering, University of Arizona, 1130N Mountain Ave, Tucson, AZ 85721, USA. Electronic address: xwu@email.arizona.edu.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wu
mesh_terms: D000906:Antibodies / Q000276:immunology*; D008055:Lipids / Q000276:immunology*; D011108:Polymers
chemical_list: D000906:Antibodies; D008055:Lipids; D011108:Polymers
keywords: Antibody immobilization; Cholesterol; Function retention; Lipid fibers; Physical adsorption