title: Expression ofMALAT1in the peripheral whole blood of patients with lung cancer.
abstract: A blood-based biomarker assay is a non-invasive way to screen that can identify lung cancer at an earlier stage to improve the clinical outcome.MALAT1is a broadly expressed, long non-coding RNA in human tissues and is overexpressed in numerous human carcinomas. The potential ofMALAT1in the whole blood of lung cancer was evaluated. In the present study, blood samples of patients with lung cancer and healthy volunteers (controls) were recruited and analyzed by quantitative polymerase chain reaction (qPCR) forMALAT1expression and clinicopathological data. Lung cancer tissues were also analyzed by qPCR. The expression ofMALAT1in the whole blood of lung cancer was lower compared to the control. The area under the receiver operator curve was 0.718 (P<0.001). Relatively, the expression ofMALAT1was stronger in the whole blood of lung cancer with metastasis compared to non-metastasis. Additionally, the whole blood with bone or brain metastasis exhibited a higher expression ofMALAT1compared to the blood with lymph node or pleura metastasis. Subsequently, a lower expression ofMALAT1was detected in metastatic lymph node tissues than that of the carcinomain situof the lung. Taken together, these results indicate thatMALAT1as a biomarker to screen lung cancer may represent a host response to lung cancer.
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Laboratories, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China ; Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China ; Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
keywords: MALAT1; lung cancer; quantitative polymerase chain reaction