title: Antioxidant and Anti-Inflammatory Phenolic Glycosides from Clematis tashiroi.
abstract: From the 95% EtOH extract of dried aerial parts of Clematis tashiroi, eight new and four known phenolic (caffeic acid, coumaric acid, ferrulic acid) glycosides were isolated and characterized. The structures of the new isolates (clematisides A-H) were elucidated by spectroscopic data interpretation as trans-4-O-(6-O-trans-caffeoyl-β-D- glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (1), trans-4-O-(6-O-trans-feruloyl-β-D-glucopyranosyll)-9-O-β-D-glucopyranosyl caffeic acid (2), trans-4-O-(6-O-trans-p-coumaroyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (3), trans-4-O-(6-O-trans-caffeoyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl p-coumaric acid (4), trans-3-O-(6-O-trans-caffeoyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (5), trans-3-O-(6-O-trans-p-coumaroyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (6), 6-(3',4'-dihydroxystyryl)-2-pyrone-4-O-(6-O-trans-caffeoyl)-β-D-glucopyranoside (7), and 6-(3',4'-dihydroxystyryl)-2-pyrone-4-O-{6-O-[4-O-(6-O-trans-caffeoyl)-β-D-glucopyranosyl]-trans-caffeoyl}-β-D-glucopyranoside (8), respectively. In a DPPH radical-scavenging test, compounds 1, 7, and 8 showed more potent antioxidant activity than that of the positive control, vitamin E. In addition, compound 7 also showed inhibitory activity in an antinitric oxide release assay.
authors.affiliation: †National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan, Republic of China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: †National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan, Republic of China.
authors.forename: Hung-Tse
authors.initials: HT
authors.lastname: Huang
authors.affiliation: ‡Endemic Species Research Institute, Nantou County 552, Taiwan, Republic of China.
authors.forename: Shih-Yen
authors.initials: SY
authors.lastname: Huang
authors.affiliation: †National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan, Republic of China.
authors.forename: Zhi-Hu
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: †National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan, Republic of China.
authors.forename: Chien-Chang
authors.initials: CC
authors.lastname: Shen
authors.affiliation: †National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan, Republic of China.
authors.forename: Wei-Jern
authors.initials: WJ
authors.lastname: Tsai
authors.affiliation: †National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan, Republic of China.|§Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan, Republic of China.|⊥Ph.D. Program for the Clinical Drug Discovery from Botanical Herbs, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan, Republic of China.
authors.forename: Yao-Haur
authors.initials: YH
authors.lastname: Kuo
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002109:Caffeic Acids / Q000494:pharmacology; D039324:Clematis / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010851:Picrates / Q000494:pharmacology; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D002109:Caffeic Acids; D006027:Glycosides; D010636:Phenols; D010851:Picrates; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl; C040048:caffeic acid