title: New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs.
abstract: From October 2013 to date, approximately 1,000 outbreaks of porcine epidemic diarrhoea virus (PEDV) have occurred in Japan. Porcine epidemic diarrhoea with non-lethal effects in piglets was identified in Tottori prefecture in October 2014. Complete genome analysis revealed that the causative pathogen, Tottori2, is a new PEDV variant with a large (582 nt) deletion in the spike gene. Phylogenetic analysis indicated that the Tottori2 PEDV strain might have been derived from the current PEDV strains circulating in domestic pigs. Moreover, the Tottori2 PEDV strain was successfully isolated in Vero cells by serial passage.
authors.affiliation: Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, Japan.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Masuda
authors.affiliation: Life Technologies Japan Ltd., Thermo Fisher Scientific, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.affiliation: Life Technologies Japan Ltd., Thermo Fisher Scientific, Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Takahashi
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ohashi
authors.affiliation: Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasato
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan. t_suzuki@affrc.go.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D064370:Spike Glycoprotein, Coronavirus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D034421:Sus scrofa / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D064370:Spike Glycoprotein, Coronavirus