title: Molecular Epidemiology and Characterization of Genotypes of Acinetobacter baumannii Isolates from Regions of South China.
abstract: The aim of this study was to analyze the molecular epidemiologic characteristics of Acinetobacter baumannii. A total of 398 isolates were collected in 7 regions of South China from January to June of 2012. Drug sensitivity was tested toward 15 commonly used antibiotics; thus, 146 multi-drug-resistant strains (resistant to more than 7 drugs) were identified, representing 36.7% of all isolates. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular subtyping. According to the PFGE results (with a cutoff of 70% similarity for the DNA electrophoretic bands), 146 strains were subdivided into 15 clusters, with cluster A being the largest (33.6%, distributed in all districts except Jiaxing). Cluster B was also widespread and included 14.4% of all strains. In addition, MLST results revealed 11 sequence types (ST), with ST208 being the most prevalent, followed by ST191 and ST729. Furthermore, 4 novel alleles and 6 novel STs were identified. Our results showed that multi-drug-resistant A. baumannii in South China shares the origin with other widespread strains in other countries. The nosocomial infections caused by A. baumannii have been severe in South China. Continuous monitoring and judicious antibiotic use are required.
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.forename: Junwan
authors.initials: J
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Zong
authors.forename: Ailing
authors.initials: A
authors.lastname: Li
authors.forename: Ruowang
authors.initials: R
authors.lastname: Pan
authors.forename: Cong
authors.initials: C
authors.lastname: Cheng
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Li
authors.forename: Liqiang
authors.initials: L
authors.lastname: Chen
authors.forename: Jianchao
authors.initials: J
authors.lastname: Ying
authors.forename: Huifen
authors.initials: H
authors.lastname: Tou
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Zhu
authors.forename: Teng
authors.initials: T
authors.lastname: Xu
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yi
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
authors.forename: Liyan
authors.initials: L
authors.lastname: Ni
authors.forename: Zuyuan
authors.initials: Z
authors.lastname: Xu
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Bao
authors.forename: Peizhen
authors.initials: P
authors.lastname: Li
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000483:Alleles; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D005810:Multigene Family; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D000900:Anti-Bacterial Agents