title: Characterization of the Prokaryotic Sodium Channel NavSp Pore with a Microfluidic Bilayer Platform.
abstract: This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (Na(v)Sp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC50 determined.
authors.affiliation: Electronics and Computer Science, University of Southampton, Southampton, SO17 1BJ, United Kingdom.
authors.forename: Shimul Chandra
authors.initials: SC
authors.lastname: Saha
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, WC1E 7HX, United Kingdom.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Henderson
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, WC1E 7HX, United Kingdom.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Powl
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, WC1E 7HX, United Kingdom.
authors.forename: B A
authors.initials: BA
authors.lastname: Wallace
authors.affiliation: Electronics and Computer Science, University of Southampton, Southampton, SO17 1BJ, United Kingdom; Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, United Kingdom.
authors.forename: Maurits R R
authors.initials: MR
authors.lastname: de Planque
authors.affiliation: Electronics and Computer Science, University of Southampton, Southampton, SO17 1BJ, United Kingdom; Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, United Kingdom.
authors.forename: Hywel
authors.initials: H
authors.lastname: Morgan
mesh_terms: D015640:Ion Channel Gating / Q000502:physiology*; D008051:Lipid Bilayers / Q000378:metabolism; D044085:Microfluidics; D011510:Proteolipids / Q000378:metabolism; D041902:Rhodobacteraceae / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D011510:Proteolipids; D015222:Sodium Channels; C020485:proteoliposomes