title: CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.
abstract: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.
authors.affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Liang
authors.affiliation: 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Bao
authors.affiliation: 3. Center for molecular imaging and Translational medicine of Xiamen University, Xiamen 361102, P. R. China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Wang
authors.affiliation: 3. Center for molecular imaging and Translational medicine of Xiamen University, Xiamen 361102, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 4. Suzhou Kowloon Hospital Shanghai Jiaotong University School of Medicine.
authors.forename: Fengchun
authors.initials: F
authors.lastname: Zhang
authors.affiliation: 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems / Q000379:methods*; D006046:Gold / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology*; D006979:Hyperthermia, Induced / Q000379:methods*; D007259:Infrared Rays; D028022:Low-Level Light Therapy; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage; D061848:Optical Imaging / Q000379:methods; D061088:Photoacoustic Techniques / Q000379:methods*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000188:drug therapy*; D016019:Survival Analysis; D000068936:Theranostic Nanomedicine / Q000379:methods; D016896:Treatment Outcome
chemical_list: D000911:Antibodies, Monoclonal; C112697:CD44v6 antigen; D018960:Hyaluronan Receptors; D006046:Gold
keywords: CD44v6 monoclonal antibody; gastric cancer stem cells (GCSCs); gold nanostars; photoacoustic imaging; photothermal therapy