title: Differential production of lignocellulolytic enzymes by a white rot fungus Termitomyces sp. OE147 on cellulose and lactose.
abstract: White-rot fungi are the only organisms known to degrade all basic wood polymers using different strategies of employing a variety of hydrolytic and oxidative enzymes. A comparative secretome analysis of Termitomyces sp. OE147 cultivated on cellulose and lactose was carried out by two-dimensional gel electrophoresis followed by MALDI-TOF/TOF-MS analysis to identify the enzymes coordinately expressed on cellulose. A total of 29 proteins, belonging to CAZy hydrolases (11), CAZy oxidoreductases (13) and some 'other' (5) proteins were identified. Among the CAZy hydrolases, a distinct repertoire of cellulolytic and hemicellulolytic enzymes were produced while among the CAZy oxidoreductases, cellobiose dehydrogenase and laccase were the predominant enzymes along with H2O2 dependent peroxidases. This coordinated expression indicated a unique and integrated system for degradation of not only crystalline cellulose but also other components of lignocellulolytic substrates, namely lignin and xylan. Activities of the identified proteins were confirmed by plate assays and activity measurements. Many of the enzyme activities were also correlated with reduction in the crystallinity index of cellulose. Based on the enhanced production of CDH, β-glucosidases and several oxidoreductases, a more prominent role of these enzymes is indicated in this fungus in cellulose breakdown.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India.
authors.forename: Humayra
authors.initials: H
authors.lastname: Bashir
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India.
authors.forename: Rishabh
authors.initials: R
authors.lastname: Gangwar
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India. Electronic address: saroj98@hotmail.com.
authors.forename: Saroj
authors.initials: S
authors.lastname: Mishra
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism; D044602:Cellulases / Q000302:isolation & purification / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism; D007785:Lactose / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055434:Termitomyces / Q000737:chemistry / Q000201:enzymology*; D014934:Wood / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D014990:Xylans; D002482:Cellulose; D008031:Lignin; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D042845:Laccase; D010544:Peroxidases; D044602:Cellulases; D007785:Lactose
keywords: Lignocellulolytic enzymes; MALDI–TOF/TOF–MS; Secretome; Termitomyces sp. OE147; Two-dimensional gel electrophoresis