title: Analysis of the UHRF1 expression in serum and tissue for gastric cancer detection.
abstract: OBJECTIVE
  To analyze the Ubiquitin-like with PHD and ring finger domains (UHRF) 1 expression in gastric cancer (GC).
  
  
  METHODS
  The concentrations of UHRF1 DNA in serum were compared between 187 GC patients and 56 healthy controls using real-time PCR. Immunohistochemical analysis using tissue microarrays was performed.
  
  
  RESULTS
  UHRF1 DNA levels were significantly higher in GC patients compared to healthy controls (p < 0.001) and have associations with age and lymph node metastasis (LNM). The UHRF1 expression was significantly higher in tumor tissue than matched normal tissues (p < 0.001).
  
  
  CONCLUSIONS
  The UHRF1 expression in serum and tissue may represent a novel biomarker for GC diagnosis and prognosis.
authors.affiliation: a Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing Medical University Cancer Hospital & Jiangsu Cancer Hospital , Nanjing , China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Ge
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gui
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D022762:CCAAT-Enhancer-Binding Proteins / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D046888:Tissue Array Analysis; D044767:Ubiquitin-Protein Ligases
chemical_list: D014408:Biomarkers, Tumor; D022762:CCAAT-Enhancer-Binding Proteins; D004273:DNA, Neoplasm; C406364:UHRF1 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Serum; UHRF1; stomach cancer; tissue microarray; tumor marker