title: Optical recording of calcium currents during impulse conduction in cardiac tissue.
abstract: We explore the feasibility of obtaining a spatially resolved picture of [Formula: see text] inward currents ([Formula: see text]) in multicellular cardiac tissue by differentiating optically recorded [Formula: see text] transients that accompany propagating action potentials. Patterned growth strands of neonatal rat ventricular cardiomyocytes were stained with the [Formula: see text] indicators Fluo-4 or Fluo-4FF. Preparations were stimulated at 1 Hz, and [Formula: see text] transients were recorded with high spatiotemporal resolution ([Formula: see text], 2 kHz analog bandwidth) with a photodiode array. Signals were differentiated after appropriate digital filtering. Differentiation of [Formula: see text] transients resulted in optically recorded calcium currents (ORCCs) that carried the temporal and pharmacological signatures of L-type [Formula: see text] inward currents: the time to peak amounted to [Formula: see text] (Fluo-4FF) and [Formula: see text] (Fluo-4), full-width at half-maximum was [Formula: see text], and ORCCs were completely suppressed by [Formula: see text][Formula: see text]. Also, and as reported before from patch-clamp studies, caffeine reversibly depressed the amplitude of ORCCs. The results demonstrate that the differentiation of [Formula: see text] transients can be used to obtain a spatially resolved picture of the initial phase of [Formula: see text] in cardiac tissue and to assess relative changes of activation/fast inactivation of [Formula: see text] following pharmacological interventions.
authors.affiliation: University of Bern , Department of Physiology, Bühlplatz 5, CH-3012 Bern, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Jousset
authors.affiliation: University of Bern , Department of Physiology, Bühlplatz 5, CH-3012 Bern, Switzerland.
authors.forename: Stephan
authors.initials: S
authors.lastname: Rohr
keywords: action potential; calcium current; cardiac tissue; fast optical measurement; fluorescent calcium indicator; heart; impulse conduction