title: Metabolomic profiles reveal key metabolic changes in heat stress-treated mouse Sertoli cells.
abstract: Heat stress (HS) is a potential harmful factor for male reproduction. However, the effect of HS on Sertoli cells is largely unknown. In this study, the metabolic changes in Sertoli cell line were analyzed after HS treatment. Metabolomic analysis revealed that carnitine, 2-hydroxy palmitic acid, nicotinic acid, niacinamide, adenosine monophosphate, glutamine and creatine were the key changed metabolites. We found the expression levels of BTB factors (Connexin43, ZO-1, Vimentin, Claudin1, Claudin5) were disrupted in TM-4 cells after HS treatment, which were recovered by the addition of carnitine. RT-PCR indicated that the mRNA levels of inflammatory cytokines (IL-1α, IL-1β, IL-6) were increased after HS treatment, and their related miRNAs (miR-132, miR-431, miR-543) levels were decreased. Our metabolomic data provided a novel understanding of metabolic changes in male reproductive cells after HS treatment and revealed that HS-induced changes of BTB factors and inflammatory status might be caused by the decreased carnitine after HS treatment.
authors.affiliation: Department of Endocrinology, Jiangyin People's Hospital, School of Medicine, Southeast University, Jiangyin 214400, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China. Electronic address: hanxiao@njmu.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Endocrinology, Jiangyin People's Hospital, School of Medicine, Southeast University, Jiangyin 214400, China; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China. Electronic address: drtangwei@aliyun.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002331:Carnitine / Q000378:metabolism; D002453:Cell Cycle; D002460:Cell Line; D062445:Claudin-1 / Q000235:genetics; D062507:Claudin-5 / Q000235:genetics; D018031:Connexin 43 / Q000235:genetics; D003401:Creatine / Q000378:metabolism; D016207:Cytokines / Q000235:genetics; D005973:Glutamine / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects*; D008297:Male; D055432:Metabolomics; D051379:Mice; D009525:Niacin / Q000378:metabolism; D009536:Niacinamide / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism*; D014746:Vimentin / Q000235:genetics; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: D062445:Claudin-1; D062507:Claudin-5; C568113:Cldn1 protein, mouse; C491511:Cldn5 protein, mouse; D018031:Connexin 43; D016207:Cytokines; C543076:GJA1 protein, mouse; D012333:RNA, Messenger; C569043:Tjp1 protein, mouse; D014746:Vimentin; D062826:Zonula Occludens-1 Protein; D005973:Glutamine; D009536:Niacinamide; D009525:Niacin; D019308:Palmitic Acid; D000249:Adenosine Monophosphate; D003401:Creatine; D002331:Carnitine
keywords: Blood-testis barrier factors; Carnitine; Heat stress; Metabolomics; Sertoli cells