title: In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiota of mice.
abstract: Lactobacillus plantarum ZDY 2013, a novel strain isolated from Chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (pH, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. Its effect on mice gut microbiota was also investigated by quantitative PCR and PCR-denaturing gradient gel electrophoresis. The results showed that ZDY 2013 can grow at pH 3.5 and survive at pH 2.0 for 6 h and at 0.45% bile salt for 3 h. The exopolysaccharide yield was up to 204±7.68 mg/L. The survival rate of ZDY 2013 in a simulated gastrointestinal tract was as high as 65.84%. Antagonism test with a supernatant of ZDY 2013 showed maximum halo of 28 mm against Listeria monocytogenes. The inhibition order was as follows: Listeria monocytogenes, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Shigella sonnei, Enterobacter sakazakii, and Staphylococcus aureus. Lactobacillus plantarum ZDY 2013 was sensitive to some antibiotics (e.g., macrolide, sulfonamides, aminoglycoside, tetracyclines and β-lactams), whereas it was resistant to glycopeptides, quinolones, and cephalosporins antibiotics. Denaturing gradient gel electrophoresis profile demonstrated that ZDY 2013 administration altered the composition of the microbiota at various intestinal loci of the mice. Moreover, the quantitative PCR test showed that the administration of ZDY 2013 enhanced the populations of Bifidobacterium and Lactobacillus in either the colon or cecum, and reduced the potential enteropathogenic bacteria (e.g., Enterococcus, Enterobacterium, and Clostridium perfringens). Lactobacillus plantarum ZDY 2013 exhibited high resistance against low pH, bile salt, and gastrointestinal fluid, and possessed antibacterial and gut microbiota modulation properties with a potential application in the development of dairy food and nutraceuticals.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Renhui
authors.initials: R
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Hengyi
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Food and Nutritional Science, School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong, China. Electronic address: npshah@hku.hk.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Shah
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China. Electronic address: weihua114@live.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001644:Bifidobacterium / Q000187:drug effects / Q000302:isolation & purification; D001647:Bile Acids and Salts / Q000378:metabolism; D002511:Cephalosporins / Q000494:pharmacology; D044083:Cronobacter sakazakii / Q000187:drug effects / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology; D006020:Glycopeptides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D019936:Probiotics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification; D015363:Quinolones / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000302:isolation & purification; D012764:Shigella sonnei / Q000187:drug effects / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D013754:Tetracyclines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D002511:Cephalosporins; D006020:Glycopeptides; D015363:Quinolones; D013754:Tetracyclines; C012235:cephalosporin 112384
keywords: Lactobacillus plantarum ZDY 2013; antagonistics; quantitative PCR; stress resistance