title: Impact of zinc supplementation on the improved fructose/xylose utilization and butanol production during acetone-butanol-ethanol fermentation.
abstract: Lignocellulosic biomass and dedicated energy crops such as Jerusalem artichoke are promising alternatives for biobutanol production by solventogenic clostridia. However, fermentable sugars such as fructose or xylose released from the hydrolysis of these feedstocks were subjected to the incomplete utilization by the strains, leading to relatively low butanol production and productivity. When 0.001 g/L ZnSO4·7H2O was supplemented into the medium containing fructose as sole carbon source, 12.8 g/L of butanol was achieved with butanol productivity of 0.089 g/L/h compared to only 4.5 g/L of butanol produced with butanol productivity of 0.028 g/L/h in the control without zinc supplementation. Micronutrient zinc also led to the improved butanol production up to 8.3 g/L derived from 45.2 g/L xylose as sole carbon source with increasing butanol productivity by 31.7%. Moreover, the decreased acids production was observed under the zinc supplementation condition, resulting in the increased butanol yields of 0.202 g/g-fructose and 0.184 g/g-xylose, respectively. Similar improvements were also observed with increasing butanol production by 130.2 % and 8.5 %, butanol productivity by 203.4% and 18.4%, respectively, in acetone-butanol-ethanol fermentations from sugar mixtures of fructose/glucose (4:1) and xylose/glucose (1:2) simulating the hydrolysates of Jerusalem artichoke tubers and corn stover. The results obtained from transcriptional analysis revealed that zinc may have regulatory mechanisms for the sugar transport and metabolism of Clostridium acetobutylicum L7. Therefore, micronutrient zinc supplementation could be an effective way for economic development of butanol production derived from these low-cost agricultural feedstocks.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: You-Duo
authors.initials: YD
authors.lastname: Wu
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Xue
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China. Electronic address: ljchen@dlut.edu.cn.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Feng-Wu
authors.initials: FW
authors.lastname: Bai
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism*; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006368:Helianthus / Q000737:chemistry / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000096:Acetone; D005632:Fructose; D000431:Ethanol; D020001:1-Butanol; D014994:Xylose; D005947:Glucose; D015032:Zinc
keywords: Acetone–butanol–ethanol fermentation; Clostridium acetobutylicum; Fructose; Xylose; Zinc supplementation