title: Aging changes agonist induced contractile responses in permeabilized rat bladder.
abstract: Aging alters bladder functions where a decrease in filling, storage and emptying is observed. These changes cause urinary incontinence, especially in women. The aim of this study is to examine how aging affects the intracellular calcium movements due to agonist-induced contractions in permeabilized female rat bladder. Urinary bladder isolated from young and old female Sprague-Dawley rats were used. Small detrusor strips were permeabilized with β-escin. The contractile responses induced with agonists were compared between young and old groups. Carbachol-induced contractions were decreased in permeabilized detrusor from old rats compared to young group. Heparin and ryanodine decreased carbachol-induced contractions in young rats where only heparin inhibited these contractions in olds. Caffeine-induced contractions but not inositol triphosphate (IP3)-induced contractions were decreased in old group compared to youngs. The cumulative calcium response curves (pCa 8-4) were also decreased in old rats. Carbachol-induced calcium sensitization responses did not alter by age where GTP-β-S and GF-109203X but not Y-27632 inhibited these responses. Carbachol-induced contractions decrease with aging in rat bladder detrusor. It can be postulated as IP3-induced calcium release (IICR) is primarily responsible for the contractions in older rats where the decrease in carbachol contractions in aging may be as a result of a decrease in calcium-induced calcium release (CICR), rather than carbachol-induced calcium sensitization.
authors.affiliation: Faculty of Pharmacy, Department of Pharmacology, Hacettepe University, 06100, Sıhhiye, Ankara, Turkey, ndurlu@hacettepe.edu.tr.
authors.forename: N Tugba
authors.initials: NT
authors.lastname: Durlu-Kandilci
authors.forename: Merve
authors.initials: M
authors.lastname: Denizalti
authors.forename: Inci
authors.initials: I
authors.lastname: Sahin-Erdemli
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects*; D002217:Carbachol / Q000494:pharmacology*; D018679:Cholinergic Agonists / Q000494:pharmacology*; D004928:Escin; D005260:Female; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D010539:Permeability; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D046509:Tissue Culture Techniques; D001743:Urinary Bladder / Q000187:drug effects* / Q000503:physiopathology*
chemical_list: D018679:Cholinergic Agonists; D011189:Potassium Chloride; D004928:Escin; D002217:Carbachol