title: Embedded 3D Photopatterning of Hydrogels with Diverse and Complex Architectures for Tissue Engineering and Disease Models.
abstract: Techniques that can create three-dimensional (3D) structures to provide architectural support for cells have a significant impact in generating complex and hierarchically organized tissues/organs. In recent times, a number of technologies, including photopatterning, have been developed to create such intricate 3D structures. In this study, we describe an easy-to-implement photopatterning approach, involving a conventional fluorescent microscope and a simple photomask, to encapsulate cells within spatially defined 3D structures. We have demonstrated the ease and the versatility of this approach by creating simple to complex as well as multilayered structures. We have extended this photopatterning approach to incorporate and spatially organize multiple cell types, thereby establishing coculture systems. Such cost-effective and easy-to-use approaches can greatly advance tissue engineering strategies.
authors.affiliation: 1 Department of Bioengineering, University of California-San Diego , La Jolla, California.
authors.forename: Shruti Krishna
authors.initials: SK
authors.lastname: Davey
authors.affiliation: 1 Department of Bioengineering, University of California-San Diego , La Jolla, California.
authors.forename: Aereas
authors.initials: A
authors.lastname: Aung
authors.affiliation: 1 Department of Bioengineering, University of California-San Diego , La Jolla, California.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Agrawal
authors.affiliation: 1 Department of Bioengineering, University of California-San Diego , La Jolla, California.
authors.forename: Han Liang
authors.initials: HL
authors.lastname: Lim
authors.affiliation: 1 Department of Bioengineering, University of California-San Diego , La Jolla, California.
authors.forename: Mrityunjoy
authors.initials: M
authors.lastname: Kar
authors.affiliation: 1 Department of Bioengineering, University of California-San Diego , La Jolla, California.|2 Institute of Engineering in Medicine, University of California-San Diego , La Jolla, California.
authors.forename: Shyni
authors.initials: S
authors.lastname: Varghese
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D018914:Cells, Immobilized / Q000166:cytology; D004195:Disease Models, Animal; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D008027:Light; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D020100:Hydrogels