title: Fucose-Mediated Transcriptional Activation of the fcs Operon by FcsR in Streptococcus pneumoniae.
abstract: In this study, we explore the impact of fucose on the transcriptome of S. pneumoniae D39. The expression of various genes and operons, including the fucose uptake PTS and utilization operon (fcs operon) was altered in the presence of fucose. By means of quantitative RT-PCR and β-galactosidase analysis, we demonstrate the role of the transcriptional regulator FcsR, present upstream of the fcs operon, as a transcriptional activator of the fcs operon. We also predict a 19-bp putative FcsR regulatory site (5'-ATTTGAACATTATTCAAGT-3') in the promoter region of the fcs operon. The functionality of this predicted FcsR regulatory site was further confirmed by promoter-truncation experiments, where deletion of half of the FscR regulatory site or full deletion led to the abolition of expression of the fcs operon.
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
authors.forename: Irfan
authors.initials: I
authors.lastname: Manzoor
authors.forename: Sulman
authors.initials: S
authors.lastname: Shafeeq
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D005643:Fucose / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D005810:Multigene Family; D009876:Operon; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D013296:Streptococcus pneumoniae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D059467:Transcriptome; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors; D005643:Fucose; D001616:beta-Galactosidase