title: Physical Activity Increases the Total Number of Bone-Marrow-Derived Mesenchymal Stem Cells, Enhances Their Osteogenic Potential, and Inhibits Their Adipogenic Properties.
abstract: Aging and sedentary lifestyle are common nowadays and are associated with the increasing number of chronic diseases. Thus, physical activity is recommended as one of three healthy behavior factors that play a crucial role in health prophylaxis. In the present study, we were interested whether physical activity influences the number and potential of bone-marrow-derived mesenchymal stem cells BMMSCs. In this study, four-week-old male C57Bl/6 mice were trained on a treadmill at progressive speeds over a 5-week period. Comparisons made between exercised (EX) and sedentary animal groups revealed (i) significantly higher number of MSCs in EX animals, (ii) elevated alkaline phosphatase (ALP) activity, (iii) increased level of osteopontin (OPN) and osteocalcin (OCL), and (iv) reduced marrow cavity fat. The results obtained support the thesis that EX may play a substantial role in the regeneration of mesenchymal tissues. Therefore, EX may represent a novel, nonpharmacological strategy of slowing down age-related decline of the musculoskeletal functions.
authors.affiliation: Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Norwida 31 Street, 50-375 Wroclaw, Poland ; Wroclaw Research Centre EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: Wroclaw Research Centre EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland ; Faculty of Biology, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śmieszek
authors.affiliation: Faculty of Biology, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Chrząstek
authors.affiliation: Faculty of Biology, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Basinska
authors.affiliation: Wroclaw Research Centre EIT+, Stablowicka 147 Street, 54-066 Wroclaw, Poland ; Faculty of Biology, University of Environmental and Life Sciences, Kożuchowska 5b Street, 50-631 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz