title: High Frequency of JCV DNA Detection in Prostate Cancer Tissues.
abstract: BACKGROUND
  Prostate cancer (PC) represents the most frequently diagnosed cancer in men. Exposure to infectious agents has been considered to induce prostatic inflammation and cancerous transformation. Controversial data exist concerning the role of the human polyomaviruses BK (BKV) and JC (JCV) in PC etiology. Therefore, a possible association between these polyomaviruses and PC was investigated.
  
  
  METHODS
  Urine, blood and fresh prostatic tissue specimens were collected from 26 patients with PC. The presence of BKV and JCV, the possible non-coding control region (NCCR) variations and the genotyping analysis of viral protein 1 (VP1) of both viruses were assessed.
  
  
  RESULTS
  Data showed a preferential viral re-activation in the urinary compartment and a statistically significant prevalence of JC viruria and of BKV in PC tissues. A BKV DDP-like NCCR sequence was isolated in two patients, whereas JCV NCCR was consistently of an archetypal structural organization. A prevalence of the European genotypes was observed for both viruses.
  
  
  CONCLUSIONS
  Our data demonstrated the presence of JCV DNA in 14/24 (58.3%) cancerous prostatic tissue specimens, confirming the results obtained in a previous study, in which JCV has been defined as common inhabitant of the prostate, and opening the discussion about its potential role in PC.
authors.affiliation: Department of Health Sciences and Infectious Diseases, Sapienza University, Rome, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Anzivino
authors.affiliation: Department of Health Sciences and Infectious Diseases, Sapienza University, Rome, Italy.
authors.forename: Donatella Maria
authors.initials: DM
authors.lastname: Rodio
authors.affiliation: Department of Health Sciences and Infectious Diseases, Sapienza University, Rome, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Mischitelli
authors.affiliation: Department of Public Health and Infectious Diseases, Pasteur Institute, Cenci-Bolognetti Foundation, Sapienza University of Rome, Rome, Italy Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA, U.S.A.
authors.forename: Anna
authors.initials: A
authors.lastname: Bellizzi
authors.affiliation: Department of Obstretics, Gynecology and Urological Sciences, Sapienza University, Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sciarra
authors.affiliation: Department of Obstretics, Gynecology and Urological Sciences, Sapienza University, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Salciccia
authors.affiliation: Department of Obstretics, Gynecology and Urological Sciences, Sapienza University, Rome, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Gentile
authors.affiliation: Department of Health Sciences and Infectious Diseases, Sapienza University, Rome, Italy Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA, U.S.A. valeria.pietropaolo@uniroma1.it.
authors.forename: Valeria
authors.initials: V
authors.lastname: Pietropaolo
mesh_terms: D000368:Aged; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D021901:DNA, Intergenic / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D007577:JC Virus / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D019562:Viral Load / Q000235:genetics
chemical_list: D021901:DNA, Intergenic; D004279:DNA, Viral
keywords: BKV; JCV; NCCR; Prostate cancer; sequence analysis