title: Layer-by-layer engineered nanocapsules of curcumin with improved cell activity.
abstract: Nanocarriers based on electrostatic Layer-by-layer (LbL) assembly of CaCO3 nanoparticles (CaCO3 NPs) was investigated. These inorganic nanoparticles was used as templates to construct nanocapsules made from films based on two oppositely charged polyelectrolytes, poly(diallyldimethylammonium chloride), and poly (sodium 4-styrene-sulfonate sodium salt), followed by core dissolution. The naked CaCO3 NPs, CaCO3 NPs coated with the polyelectrolytes and hollow nanocapsules were found with hexagonal shape with average sizes of 350-400 nm. A reversal of the surface charge between positive to negative zeta potential values was found, confirming the adsorption of polyelectrolytes. The loading efficiency and release of curcumin were controlled by the hydrophobic interactions between the drug and the polyelectrolyte matrix of the hollow nanocapsules. The quantity of curcumin released from hollow nanocapsules was found to increase under acidic environments, which is a desirable for anti-cancer drug delivery. The hollow nanocapsules were found to localize in the cytoplasm and nucleus compartment of Hela cancer cells after 24 h of incubation. Hollow nanocapsules were non-toxic to human fibroblast cells. Furthermore, curcumin loaded hollow nanocapsules exhibited higher in vitro cell inhibition against Hela cells than that of free curcumin, suggesting that polyelectrolyte based-hollow nanocapsules can be utilized as new carriers for drug delivery.
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Paveenuch
authors.initials: P
authors.lastname: Kittitheeranun
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Warayuth
authors.initials: W
authors.lastname: Sajomsang
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Sarunya
authors.initials: S
authors.lastname: Phanpee
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Alongkot
authors.initials: A
authors.lastname: Treetong
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Tuksadon
authors.initials: T
authors.lastname: Wutikhun
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
authors.forename: Kunat
authors.initials: K
authors.lastname: Suktham
authors.affiliation: Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Satit
authors.initials: S
authors.lastname: Puttipipatkhachorn
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand. Electronic address: uracha@nanotec.or.th.
authors.forename: Uracha Rungsardthong
authors.initials: UR
authors.lastname: Ruktanonchai
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D065546:Drug Liberation; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D053769:Nanocapsules; D011095:Polyethylenes; D011137:Polystyrenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; C003321:polystyrene sulfonic acid; D002119:Calcium Carbonate; D003474:Curcumin
keywords: Calcium carbonate; Curcumin; Hollow nanocapsules; Layer-by-layer; Polyelectrolyte multilayer