title: Protection of mice against Staphylococcus aureus infection by a recombinant protein ClfA-IsdB-Hlg as a vaccine candidate.
abstract: Staphylococcus aureus is one of the most important causes of nosocomial infections. An effective vaccine to prevent S. aureus infections is urgently required due to the dramatic increase in the number of antibiotic-resistant strains. In this report, we evaluated a newly recombinant protein composed of selected antigenic regions of clumping factor A (ClfA), iron surface determinant B (IsdB) and gamma hemolysin B (HlgB) of S. aureus and sequence coding for hydrophobic linkers between three domains. The recombinant gene was constructed in pET-28a (+) and expressed in Escherichia coli BL21. In addition, sequence coding for a His(6)-tag was added followed by a hybrid procedure of nickel chelate protein purification. Immunization of BALB/c mice with the recombinant protein ClfA-IsdB-Hlg evoked antigen-specific antibodies that could opsonize S. aureus cells, enhancing in vitro phagocytosis by macrophages. Vaccination with the recombinant protein also reduced the bacterial load recovered from mice spleen samples and increased survival following the intraperitoneal challenge with pathogenic S. aureus compared to the control mice. Our results showed that the recombinant protein ClfA-IsdB-Hlg is a promising vaccine candidate for the prevention of S. aureus bacteremia infections.
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14111-115, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Delfani
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14111-115, Tehran, Iran. mmmobarez@modares.ac.ir.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Mohabati Mobarez
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Imani Fooladi
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Amani
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. emaneini@tums.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D027682:Cation Transport Proteins / Q000235:genetics / Q000276:immunology*; D003030:Coagulase / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D027682:Cation Transport Proteins; C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D006460:Hemolysin Proteins; C510010:IsdB protein, Staphylococcus aureus; D011993:Recombinant Fusion Proteins; D013209:Staphylococcal Vaccines; D014614:Vaccines, Synthetic; C037956:gamma-hemolysin, Staphylococcus aureus
keywords: Clumping factor A (ClfA); Gamma hemolysin B (HlgB) recombinant protein; Immunogenicity; Iron surface determinant B (IsdB); Staphylococcus aureus