title: Application of Intermittent Microwave Irradiation to Western Blot Analysis.
abstract: We established a shortened protocol for Western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.
authors.affiliation: Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Liu
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004867:Equipment Design; D008297:Male; D008872:Microwaves; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000199:Actins; D000906:Antibodies