title: Antioxidant potential of indigenous cyanobacterial strains in relation with their phenolic and flavonoid contents.
abstract: Antioxidant activities of eight indigenous cyanobacterial strains belonging to the genera Oscillatoria, Chroococcidiopsis, Leptolyngbya, Calothrix, Nostoc and Phormidium were studied in relation with their phenolic and flavonoid contents, ranging 3.9-12.6 mg GAE g(-1) and 1.7-3.44 mg RE g(-1). The highest activities were shown by Leptolyngbya sp. SI-SM (EC50 = 63.45 and 67.49 μg mL(-1)) and Calothrix sp. SI-SV (EC50 = 65.79 and 69.38 μg mL(-1)) calculated with ABTS and DPPH assays. Significant negative correlations were seen between total phenolic and flavonoid contents and the antioxidant activities in terms of EC50 values. Furthermore, HPLC detected 15 phenolic compounds with total concentrations ranging from 277.3 to 829.7 μg g(-1). The prevalent compounds in most of the strains were rutin, tannic acid, orcinol, phloroglucinol and protocatechuic acid. Cyanobacterial strains showed high potential as a good source of phenolic compounds with potent antioxidative potential which could be beneficial for food, cosmetic and pharmaceutical industries.
authors.affiliation: a Department of Microbiology and Molecular Genetics , University of the Punjab , Quaid-e-Azam Campus, Lahore 54590 , Pakistan.
authors.forename: Saadia
authors.initials: S
authors.lastname: Ijaz
authors.affiliation: a Department of Microbiology and Molecular Genetics , University of the Punjab , Quaid-e-Azam Campus, Lahore 54590 , Pakistan.|b Department of Microbiology and Molecular Genetics , The Women University , Multan 60000 , Punjab , Pakistan.
authors.forename: Shahida
authors.initials: S
authors.lastname: Hasnain
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D062385:Hydroxybenzoates / Q000302:isolation & purification; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010696:Phloroglucinol / Q000302:isolation & purification; D012118:Resorcinols / Q000302:isolation & purification; D012431:Rutin / Q000302:isolation & purification; D013634:Tannins / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; D012118:Resorcinols; D013634:Tannins; C009091:protocatechuic acid; C005282:orcinol; D012431:Rutin; D010696:Phloroglucinol
keywords: ABTS; DPPH; HPLC; antioxidation; flavonoids; phenolics