title: A role for the small GTPase Rac1 in vaccinia actin-based motility.
abstract: Vaccinia virus dissemination relies on the recruitment of the nucleation promoting factor N-WASP underneath cell-associated extracellular virus (CEVs) and subsequent recruitment and activation of the ARP2/3 complex, a major actin nucleator of the host cell. We have recently discovered that, in addition to the N-WASP/ARP2/3 pathway, vaccinia actin-based motility also relies on the small GTPase Rac1 and its downstream effector the formin-type actin nucleator FHOD1. Here we discuss the potential signaling mechanisms supporting the integration of the N-WASP/ARP2/3 and Rac1/FHOD1 pathways. We suggest the existence of a receptor tyrosine kinase family member that would integrate the Src-dependent activation of the N-WASP/ARP2/3 pathway and the GTP exchange factor-dependent activation of the Rac1/FHOD1 pathway.
authors.affiliation: a Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Universidad Nacional de San Martín-CONICET ; San Martín , Buenos Aires , Argentina.
authors.forename: Diego E
authors.initials: DE
authors.lastname: Alvarez
authors.forename: Hervé
authors.initials: H
authors.lastname: Agaisse
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D006801:Humans; D014615:Vaccinia / Q000378:metabolism* / Q000821:virology; D014616:Vaccinia virus / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism; D057074:Virus Release; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D014764:Viral Proteins; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; D020830:rac1 GTP-Binding Protein
keywords: ARP2/3 complex; Dissemination; FHOD1; N-WASP; Rac1; actin-based motility; spread from cell to cell; vaccinia