title: A study of the role of glucose transporter 1 (Glut1) in white spot syndrome virus (WSSV) infection.
abstract: White spot syndrome virus (WSSV) is a large enveloped DNA virus, and it causes a serious disease that has led to severe mortalities of cultured shrimps in many countries. To determine the mechanism of virus entry into the cell and to establish an antiviral strategy, the cell receptor for virus entry and receptor binding protein should be identified. A shrimp cell surface protein, glucose transporter1 (Glut1), was found to interact with WSSV in previous study. In this study, this Glut1 was confirmed to have the ability of transporting glucose, and this gene can also be found in other shrimp species. The interaction between Glut1 and some other WSSV envelope proteins in the infectome structure was verified by far western blot and His pull down assay. In vitro and in vivo neutralization using recombinant partial Glut1 revealed that the large extracellular portion of Glut1 could delay WSSV infection. Also, shrimps which were knocked-down Glut1 gene by treated with dsRNA before WSSV challenge showed decreased mortality. These results indeed provide a direction to develop efficient antiviral strategies or therapeutic methods by using Glut1.
authors.affiliation: Institute of Marine Biology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan.
authors.forename: Huai-Ting
authors.initials: HT
authors.lastname: Huang
authors.affiliation: Department of Bioscience and Biotechnology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan.
authors.forename: Hoi-Ling
authors.initials: HL
authors.lastname: Chan
authors.affiliation: Department of Bioscience and Biotechnology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan.
authors.forename: Tsai-Yen
authors.initials: TY
authors.lastname: Shih
authors.affiliation: Institute of Marine Biology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan; Department of Bioscience and Biotechnology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan. Electronic address: joechen@ntou.edu.tw.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Chen
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D051272:Glucose Transporter Type 1; D014764:Viral Proteins
keywords: Glucose transporter1 (Glut1); Infectome; In vitro neutralization; In vivo neutralization; White spot syndrome virus (WSSV); dsRNA knock-down