title: Preparation and Characterization of Dictyophora indusiata Polysaccharide-Zinc Complex and Its Augmented Antiproliferative Activity on Human Cancer Cells.
abstract: Dictyophora indusiata polysaccharide (DP1) was successfully chelated with zinc chloride to achieve its enhanced antiproliferative activity. The obtained DP1-Zn complex showed significant antiproliferative activity (18.1 ± 2.84% viability of MCF-7 cells at 250 μg/mL) on a group of human cancer cell lines through induction of apoptosis. The pro-apoptotic action of DP1-Zn was confirmed by morphological changes including chromatin condensation, DNA breakage, and S phase cell cycle arrest in human breast adenocarcinoma cells (MCF-7). The DP1-Zn-induced apoptotic pathways were characterized by the activation of caspases-3, -8, and -9, mitochondrial dysfunction, and reactive oxygen species (ROS) overproduction (305 ± 7.06% production of control at 250 μg/mL). This study suggested that DP1-Zn can be developed as a candidate for cancer treatment and prevention, especially human breast adenocarcinoma.
authors.affiliation: †College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou 510640, China.
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Liao
authors.affiliation: †College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou 510640, China.
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Lu
authors.affiliation: §Department of Food Science and Engineering, Jinan University, 601 West Huangpu Road, Guangzhou 510632, China.
authors.forename: Junning
authors.initials: J
authors.lastname: Fu
authors.affiliation: †College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou 510640, China.
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Ning
authors.affiliation: †College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou 510640, China.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Yang
authors.affiliation: †College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou 510640, China.
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001487:Basidiomycota / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D015032:Zinc
keywords: Dictyophora indusiata polysaccharides; antiproliferative activity; apoptosis; human breast adenocarcinoma cells; zinc chloride