title: Development of a multimodal imaging probe by encapsulating iron oxide nanoparticles with functionalized amphiphiles for lymph node imaging.
abstract: OBJECTIVE
  We tried to develop a multimodal iron oxide nanoparticles (IO NP) imaging probe by an encapsulation method using specific amphiphiles for (68)Ga-labeling and lymph node-targeting.
  
  
  METHODS
  Nanoparticles (NPs) were encapsulated with a solution containing polysorbate 60 and the amphiphiles. The prepared NPs were labeled with (68)Ga and tested in vitro and in vivo.
  
  
  RESULTS
  Prepared 1,4,7-triazacyclononane-1,4,7-triacetic acid-IO-Mannose (NOTA-IO-Man) showed a narrow size distribution, and no significant aggregation or degradation under harsh conditions. The relaxivity coefficient of (68)Ga-NOTA-IO-Man was higher than that of ferumoxide. The accumulation of (68)Ga-NOTA-IO-Man in the lymph node after injection into rat's footpad was confirmed by both positron emission tomography and MRI.
  
  
  CONCLUSIONS
  We successfully developed PET/MRI dual-modality imaging probe targeting lymph nodes by using the facile encapsulation method.
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: Bo Yeun
authors.initials: BY
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: Sudhakara Reddy
authors.initials: SR
authors.lastname: Seelam
authors.affiliation: Department of Radiology, Seoul National University Hospital, 110-744, Seoul, South Korea.
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Jeon
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: Yun-Sang
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.|Cancer Research Institute, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: June-Key
authors.initials: JK
authors.lastname: Chung
authors.affiliation: Department of Radiology, Seoul National University Hospital, 110-744, Seoul, South Korea.
authors.forename: Young Il
authors.initials: YI
authors.lastname: Kim
authors.affiliation: Department of Nuclear Medicine & Institute of Radiation Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea.|Cancer Research Institute, Seoul National University College of Medicine, 110-799, Seoul, South Korea.
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Jeong
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D005710:Gallium Radioisotopes / Q000737:chemistry; D008198:Lymph Nodes / Q000166:cytology* / Q000000981:diagnostic imaging*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D064847:Multimodal Imaging / Q000379:methods; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D049268:Positron-Emission Tomography / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000138:chemical synthesis
chemical_list: D003287:Contrast Media; D005710:Gallium Radioisotopes; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D013501:Surface-Active Agents
keywords: 68Ga; PET/MRI; encapsulation; iron oxide nanoparticles; tween60