title: Metabolic regulation of magnolol on the nuclear receptor, liver X receptor.
abstract: The aim of the present study was to investigate whether magnolol, the essential component of the traditional Chinese medicine,Magnolia officinalis, can pass through liver X receptor α (LXRα), to subsequently play an important role in the lipid metabolic balance. Using a HepG2 human hepatoma cell line, mammalian cellular one-hybridization and mammalian cell transcriptional activation experiments were performed to detect the combination degree of magnolol at different concentrations with LXRα, and assess the transcriptional activity. In addition, using a THP-1 human monocytic cell line, quantitative polymerase chain reaction was performed to assess the effect on the expression levels of downstream genes. Magnolol was shown to dose-dependently combine with LXRα, and subsequently regulate the transcriptional activity of LXRα. In addition, magnolol was found to adjust the expression of associated LXRα downstream genes in the macrophages. In conclusion, magnolol was demonstrated to affect LXRα, which may outline a new molecular mechanism through which magnolol exerts a lipid-lowering function.
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China.
authors.forename: N A
authors.initials: NA
authors.lastname: Xie
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Internal Medicine, Luyi Xian People's Hospital, Zhoukou, Henan 477200, P.R. China.
authors.forename: Anran
authors.initials: A
authors.lastname: Guo
authors.affiliation: Department of Internal Medicine, Luyi Xian People's Hospital, Zhoukou, Henan 477200, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: DU
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China.
authors.forename: Guotian
authors.initials: G
authors.lastname: Yin
keywords: Magnolia officinalis; cholesterol; liver X receptor α; magnolol