title: Vibrio cholerae pathogen from the freshwater-cultured whiteleg shrimp Penaeus vannamei and control with Bdellovibrio bacteriovorus.
abstract: Vibriosis has become a major global economic problem in freshwater-farmed whiteleg shrimp (Penaeus vannamei). The prevention and control of vibriosis are now priority research topics. In this study, a pathogenic strain (QH) was isolated from vibriosis-infected freshwater-farmed P. vannamei that resulted in leg yellowing and was identified as a Vibrio cholerae isolate through phylogenetic analysis and the API 32GN system. A phylogenetic tree that was constructed using the neighbor-joining method further confirmed the QH isolate as a V. cholerae strain. A virulent outer membrane protein (ompU) gene was found to be present in the QH isolate, which further confirmed its pathogenicity. In addition, Bdellovibrio bacteriovorus conferred significant protection against V. cholerae: B. bacteriovorus exhibited significant bacteriolytic effects on the V. cholerae pathogen, possessed a wide prey range that included Vibrio pathogens, and displayed a positive protective efficacy against experimental V. cholerae infection in P. vannamei. To the best of our knowledge, this is the first report of the control of shrimp pathogen V. cholerae with B. bacteriovorus.
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture of PR China, Shanghai Engineering Research Center of Aquaculture, Shanghai University Knowledge Service Platform, Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206), National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, PR China. Electronic address: hpcao@shou.edu.cn.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Cao
authors.affiliation: Marine and Fisheries Research Institute of Lianyungang, Lianyungang 222044, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: An
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture of PR China, Shanghai Engineering Research Center of Aquaculture, Shanghai University Knowledge Service Platform, Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206), National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, PR China. Electronic address: 397746729@qq.com.
authors.forename: Shan
authors.initials: S
authors.lastname: He
mesh_terms: D000818:Animals; D001501:Bdellovibrio; D005798:Genes, Bacterial; D033561:Penaeidae / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics* / Q000472:pathogenicity*
keywords: Bdellovibrio bacteriovorus; Control; Freshwater-cultured Penaeus vannamei; Leg yellowing; Vibrio cholerae