title: Multiplex MicroRNA Detection on a Power-free Microfluidic Chip with Laminar Flow-assisted Dendritic Amplification.
abstract: MicroRNA (miRNA) profile-based point-of-care (POC) diagnostic methods have attracted considerable attention. In our laboratory, singleplex miRNA detection on a power-free poly(dimethylsiloxane) (PDMS) microfluidic chip with laminar flow-assisted dendritic amplification (LFDA) has been developed. In this study, to obtain the miRNA profile and to improve the reliability of the diagnosis, multiplex miRNA detection on the same system is demonstrated without compromising any advantages of the singleplex miRNA detection. The limit of detection (LOD) was at the femto- to picomolar level and the assay time was 20 min. The sensitivity, rapidity, and portability of the microfluidic chip are adequate for POC diagnosis.
authors.affiliation: Bioengineering Laboratory, RIKEN.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ishihara
authors.forename: Kazuki
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hosokawa
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D001483:Base Sequence; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D019095:Point-of-Care Systems
chemical_list: D004129:Dimethylpolysiloxanes; D035683:MicroRNAs; C013830:baysilon