title: Preparation and characterization of an immunoaffinity column for the selective extraction of aflatoxin B1 in 13 kinds of foodstuffs.
abstract: A rapid and reliable immunoaffinity column (IAC) clean-up based ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of aflatoxin B1 (AFB1) in cereals, peanuts, vegetable oils and Chinese traditional food products like sufu and lobster sauce. The immunoaffinity column of AFB1 (AFB1-IAC) was prepared by coupling CNBr-activated Sepharose-4B with the anti-AFB1 monoclonal antibody. The column capacity of IAC was over 260ng/mL gel. Samples were extracted with methanol-water (60:40, v/v) and the extracts were then purified on an AFB1-IAC before UPLC-MS/MS analysis. The average recoveries of AFB1 in spiked samples at levels of 1.0, 5.0 and 10.0μg/kg ranged from 72% to 98%, with the relative standard deviations of 1.2-9.3% (n=6). The limits of qualification ranged from 0.07 to 0.23μg/kg, which were below the MRLs of AFB1 in the matrices evaluated. In this work, the developed method was suitable for the determination of trace AFB1 residues in 13 kinds of foodstuffs.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xie
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: He
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Fan
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Health Science Center, Shenzhen University, Shenzhen 518060, China.
authors.forename: Wen-Xiao
authors.initials: WX
authors.lastname: Jiang
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xing-Yao
authors.initials: XY
authors.lastname: Pei
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Shuang-Yang
authors.initials: SY
authors.lastname: Ding
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: haiyang@cau.edu.cn.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Jiang
mesh_terms: D000348:Aflatoxins / Q000737:chemistry* / Q000302:isolation & purification*; D010367:Arachis / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014675:Vegetables / Q000737:chemistry*
chemical_list: D000348:Aflatoxins
keywords: Aflatoxin B(1); Foodsutffs; Immunoaffinity column; UPLC–MS/MS