title: [C3H10 T1/2 cells over-expressing CCR7 have intensive immunosuppressive effect].
abstract: OBJECTIVE
  To evaluate whether the chemokine (C-C motif) receptor 7 (CCR7) over-expressing C3H10 T1/2 mesenchymal stem cells are more efficient in immunosuppression effects than the original ones in skin grafts in mice.
  
  
  METHODS
  The C3H10 T1/2 cells were amplified and transfered with EGFP-CCR7 gene using lentivirus. The skin grafts were harvested from donators (C57BL/6 mice) and then transplanted to recipients (BALB/c mice). The recipients were divided into 4 groups randomly: CCR7⁺ C3H10 T1/2 group (1 × 10⁶ CCR7⁺ C3H10 T1/2 cells suspended in 1 mL PBS were injected into the recipient mice by caudal vein), C3H10 T1/2 group (1 × 10⁶ C3H10 T1/2 cells were injected into the recipient mice after skin graft), allogenic control group (the mice were injected with 1 mL normal saline), and congenic control group (the BALB/c mice that had received the skin from the congenic ones were injected with 1 mL normal saline). The skin graft survival condition and histopathological changes were observed on the 12th postoperative day. The Th17 cells and regulatory T cells (Tregs) in the lymphocytes of spleen were detected by mouse Th17/Treg phenotyping kit.
  
  
  RESULTS
  On the 12th postoperative day, the skin surface features, histopathologic changes and flow cytometry results indicated that the skin graft survival condition of the allografts treated with CCR7⁺ C3H10 T1/2 or C3H10 T1/2 cells were better than that of the allogenic control group. Compared with C3H10 T1/2 cells, CCR7⁺ C3H10 T1/2 cells had better skin graft survival condition and weaker immune response.
  
  
  CONCLUSIONS
  CCR7⁺ C3H10 T1/2 cells induce more intensive immunosuppressive effects than original C3H10 T1/2 cells. CCR7⁺ C3H10 T1/2 cells significantly inhibit inflammatory reaction and improve the living state of skin allograft.
authors.affiliation: Department of Plastic and Reconstructive Surgery, PLA General Hospital, Beijing 100853, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medicine, Beijing 100850, China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medicine, Beijing 100850, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
authors.affiliation: Military Institute of Organ Transplantation, PLA 309 Hospital, Beijing Key Laboratory of Organ Transplantation and Immunoregulation, Beijing 100091, China.
authors.forename: Zhijia
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Military Institute of Organ Transplantation, PLA 309 Hospital, Beijing Key Laboratory of Organ Transplantation and Immunoregulation, Beijing 100091, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Military Institute of Organ Transplantation, PLA 309 Hospital, Beijing Key Laboratory of Organ Transplantation and Immunoregulation, Beijing 100091, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medicine, Beijing 100850, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Plastic and Reconstructive Surgery, PLA General Hospital, Beijing 100853, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
mesh_terms: D000818:Animals; D002452:Cell Count; D015870:Gene Expression; D006085:Graft Survival / Q000276:immunology; D049452:Green Fluorescent Proteins / Q000235:genetics; D007165:Immunosuppression Therapy / Q000379:methods*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D054400:Receptors, CCR7 / Q000235:genetics*; D016038:Skin Transplantation / Q000009:adverse effects; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology; D014162:Transfection
chemical_list: D054400:Receptors, CCR7; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins