title: The Western Blot.
abstract: Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative or semiquantitative identification of specific proteins and their molecular weight from a complex mixture. This chapter will outline the requisite steps including gel electrophoresis of a protein sample, transfer of protein from a gel to a membrane support, and immunodetection of a target antigen.
authors.affiliation: Department of Neurosciences, School of Medicine, University of California San Diego (UCSD), 9500 Gilman Drive (BRF2), La Jolla, CA, 92093, USA, thnasko@ucsd.edu.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Hnasko
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hnasko
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008970:Molecular Weight; D011145:Polyvinyls; D011506:Proteins / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D005465:Fluorocarbon Polymers
chemical_list: D000906:Antibodies; D003101:Collodion; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers