title: Metabolic shifts induced by human H460 cells in tumor-bearing mice.
abstract: Tumor markers are most popularly used in diagnosis of various cancers clinically. However, the confounding factors of individual background diversities, such as genetics, food preferences, living styles, physical exercises, etc., greatly challenge the identification of tumor markers. Study of the metabolic impact of inoculated tumors on model animals can facilitate the identification of metabolomic markers relevant to tumor insult. In this study, serum metabolites from nude mice (n = 14) inoculated with human H460 cells (human nonsmall cell lung carcinoma) were profiled using gas chromatography time-of-flight mass spectrometry. The mice with inoculated tumors showed an obviously different metabolic pattern from the control; identification of the discriminatory metabolites suggested the metabolic perturbation of free fatty acids, amino acids, glycolysis and tricarboxylic acid (TCA) cycle turnover. The significantly decreased TCA intermediates, free fatty acids, 3-hydroxybutyric acid and fluctuating amino acids (t-test, p < 0.05) in serum of tumor-bearing mice characterized the metabolic impact of local inoculated H460 tumor cells on the whole system. This indicates that they are candidate metabolomic markers for translational study of lung cancer, clinically.
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.|Clinical Pharmacology Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Suzhou Institute for Food and Drug Control, Suzhou, 215104, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Cao
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.affiliation: Nanjing Medical University, Nanjing, 211166, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Aa
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Aa
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism*; D025341:Principal Component Analysis; D014233:Tricarboxylic Acids / Q000032:analysis / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D014233:Tricarboxylic Acids
keywords: H460 tumor cells; metabolomics; nude mice; tumor markers