title: A member of the HSP90 family from ovine Babesia in China: molecular characterization, phylogenetic analysis and antigenicity.
abstract: Heat shock protein 90 (HSP90) is a key component of the molecular chaperone complex essential for activating many signalling proteins involved in the development and progression of pathogenic cellular transformation. A Hsp90 gene (BQHsp90) was cloned and characterized from Babesia sp. BQ1 (Lintan), an ovine Babesia isolate belonging to Babesia motasi-like group, by screening a cDNA expression library and performing rapid amplification of cDNA ends. The full-length cDNA of BQHsp90 is 2399 bp with an open reading frame of 2154 bp encoding a predicted 83 kDa polypeptide with 717 amino acid residues. It shows significant homology and similar structural characteristics to Hsp90 of other apicomplex organisms. Phylogenetic analysis, based on the HSP90 amino acid sequences, showed that the Babesia genus is clearly separated from other apicomplexa genera. Five Chinese ovine Babesia isolates were divided into 2 phylogenetic clusters, namely Babesia sp. Xinjiang (previously designated a new species) cluster and B. motasi-like cluster which could be further divided into 2 subclusters (Babesia sp. BQ1 (Lintan)/Babesia sp. Tianzhu and Babesia sp. BQ1 (Ningxian)/Babesia sp. Hebei). Finally, the antigenicity of rBQHSP90 protein from prokaryotic expression was also evaluated using western blot and enzyme-linked immunosorbent assay (ELISA).
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Guiquan
authors.initials: G
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Aihong
authors.initials: A
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Youquan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Luo
authors.affiliation: LUNAM Université,Oniris,Ecole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique,UMR Biologie,Epidémiologie et Analyse de Risque en santé animale,CS 40706,F-44307 Nantes,France.
authors.forename: Alain
authors.initials: A
authors.lastname: Chauvin
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1,Lanzhou,Gansu 730046,People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
authors.affiliation: LUNAM Université,Oniris,Ecole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique,UMR Biologie,Epidémiologie et Analyse de Risque en santé animale,CS 40706,F-44307 Nantes,France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Moreau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001403:Babesia / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001404:Babesiosis / Q000469:parasitology*; D001483:Base Sequence; D002681:China; D000939:Epitopes; D015723:Gene Library; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*
chemical_list: D000939:Epitopes; D018841:HSP90 Heat-Shock Proteins; D015800:Protozoan Proteins
keywords: Babesia motasi; Babesia sp. BQ1 (Lintan); HSP90; antigenicity; phylogenetic analysis