title: Protein secondary-structure description with a coarse-grained model.
abstract: A coarse-grained geometrical model for protein secondary-structure description and analysis is presented which uses only the positions of the C(α) atoms. A space curve connecting these positions by piecewise polynomial interpolation is constructed and the folding of the protein backbone is described by a succession of screw motions linking the Frenet frames at consecutive C(α) positions. Using the ASTRAL subset of the SCOPe database of protein structures, thresholds are derived for the screw parameters of secondary-structure elements and demonstrate that the latter can be reliably assigned on the basis of a C(α) model. For this purpose, a comparative study with the widely used DSSP (Define Secondary Structure of Proteins) algorithm was performed and it was shown that the parameter distribution corresponding to the ensemble of all pure C(α) structures in the RCSB Protein Data Bank matches that of the ASTRAL database. It is expected that this approach will be useful in the development of structure-refinement techniques for low-resolution data.
authors.affiliation: Centre de Biophysique Moléculaire, CNRS, Rue Charles Sadron, 45071 Orléans, France.
authors.forename: Gerald R
authors.initials: GR
authors.lastname: Kneller
authors.affiliation: Centre de Biophysique Moléculaire, CNRS, Rue Charles Sadron, 45071 Orléans, France.
authors.forename: Konrad
authors.initials: K
authors.lastname: Hinsen
mesh_terms: D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D050661:Sperm Whale; D050995:Voltage-Dependent Anion Channel 1 / Q000737:chemistry
chemical_list: D009211:Myoglobin; D011506:Proteins; C494762:VDAC1 protein, human; D050995:Voltage-Dependent Anion Channel 1
keywords: ScrewFit; coarse-grained model; secondary-structure description