title: Altered post-mortem metabolism identified in very fast chilled lamb M. longissimus thoracis et lumborum using metabolomic analysis.
abstract: The aim of this experiment was to use metabolomic techniques to investigate the energy metabolism in lamb M. longissimus thoracis et lumborum subjected to very fast chilling (VFC) post-mortem. The tissue was prepared by 2 different operators and subjected to very fast chilling (less than 0°C within 1.5h of slaughter) or typical chilling regimes (Control; 0°C within 22h of slaughter). Non-targeted metabolomic analysis ((1)H NMR) and targeted analysis ((31)P NMR, HPLC-PDA and HPLC-MS/MS) were used to examine the change in muscle metabolites post-mortem. One VFC treatment, which resulted in a colder core temperature and more tender meat, had higher levels of glycolytic intermediate metabolites pre-rigor as well as more of the end-products of adenosine and nicotine nucleotide metabolism pre-rigor, relative to conventionally chilled treatments. In conclusion, VFC to less than 0°C within 1.5h of slaughter causes considerable changes in metabolism and rigor onset, which are associated with tender meat.
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Vic 3010, Australia; Cooperative Animal Research Centre for Sheep Industry Innovation, CJ Hawkins Homestead Building, University of New England, Armidale, NSW 2351, Australia; Future Farming Research Division, Department of Primary Industries, 600 Sneydes Rd, Werribee, Vic 3030, Australia. Electronic address: Robyn.Warner@unimelb.edu.au.
authors.forename: Robyn D
authors.initials: RD
authors.lastname: Warner
authors.affiliation: Cooperative Animal Research Centre for Sheep Industry Innovation, CJ Hawkins Homestead Building, University of New England, Armidale, NSW 2351, Australia; Department of Agriculture and Food WA, Baron Hay Court South Perth, WA 6151, Australia.
authors.forename: Robin H
authors.initials: RH
authors.lastname: Jacob
authors.affiliation: AgResearch Limited, Ruakura Research Centre, East Street, Private Bag 3123, Hamilton 3240, New Zealand.
authors.forename: Katja
authors.initials: K
authors.lastname: Rosenvold
authors.affiliation: Cooperative Animal Research Centre for Sheep Industry Innovation, CJ Hawkins Homestead Building, University of New England, Armidale, NSW 2351, Australia; Biosciences Research Division, Department of Primary Industries, 1 Park Drive, Bundoora, Vic 3083, Australia.
authors.forename: Simone
authors.initials: S
authors.lastname: Rochfort
authors.affiliation: Future Farming Research Division, Department of Primary Industries, 1 Park Drive, Bundoora, Vic 3083, Australia.
authors.forename: Craige
authors.initials: C
authors.lastname: Trenerry
authors.affiliation: Future Farming Research Division, Department of Primary Industries, 1 Park Drive, Bundoora, Vic 3083, Australia.
authors.forename: Tim
authors.initials: T
authors.lastname: Plozza
authors.affiliation: Cooperative Animal Research Centre for Sheep Industry Innovation, CJ Hawkins Homestead Building, University of New England, Armidale, NSW 2351, Australia; Biosciences Research Division, Department of Primary Industries, 1 Park Drive, Bundoora, Vic 3083, Australia.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: McDonagh
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008460:Meat / Q000032:analysis*; D055432:Metabolomics; D018482:Muscle, Skeletal / Q000737:chemistry*; D010725:Phosphocreatine / Q000378:metabolism; D011180:Postmortem Changes; D012518:Sarcomeres / Q000737:chemistry; D034561:Sheep, Domestic; D053719:Tandem Mass Spectrometry
chemical_list: D010725:Phosphocreatine; D000241:Adenosine
keywords: Lamb; Meat tenderness; Metabolomics; Rapid chilling