title: Comparison of industrially viable pretreatments to enhance soybean straw biodegradability.
abstract: This study explores acid and alkaline pretreatments in order to enhance soybean straw biodegradability. The effects of sulfuric acid and sodium hydroxide for different pretreatment times at 30°C and 121°C on biomass dissolution and the subsequent enzymatic hydrolysis were investigated. The highest total conversion to reducing sugars of 93.9% was attained when soybean straw was pretreated with acid (4% H2SO4, 121°C, 1 h) and subsequently subjected to the enzymatic process. However, conversion of 86.5%, were reached only with the hydrolysis of the pretreated residue using mild conditions, (0.5% NaOH, 30°C, 48 h), involving the reduction cost of the process. In addition to this, this result was dramatically decreased when pectinase was removed from the enzyme cocktail. It has been also demonstrated that the reduction of the enzyme loading to less than half allowed obtaining about 96% of the reducing sugars attained with the highest enzyme dose.
authors.affiliation: Departamento de Ingeniería Química, Instituto Superior Politécnico José Antonio Echeverría, Cujae, Ave. 114 No. 11901, Marianao 19390, Cuba. Electronic address: ecabrera@quimica.cujae.edu.cu.
authors.forename: Emir
authors.initials: E
authors.lastname: Cabrera
authors.affiliation: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real, 11510 Cádiz, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Muñoz
authors.affiliation: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real, 11510 Cádiz, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Martín
authors.affiliation: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real, 11510 Cádiz, Spain.
authors.forename: Ildefonso
authors.initials: I
authors.lastname: Caro
authors.affiliation: Departamento de Ingeniería Química, Instituto Superior Politécnico José Antonio Echeverría, Cujae, Ave. 114 No. 11901, Marianao 19390, Cuba.
authors.forename: Caridad
authors.initials: C
authors.lastname: Curbelo
authors.affiliation: Departamento de Ingeniería Química y Tecnología de Alimentos, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real, 11510 Cádiz, Spain.
authors.forename: Ana B
authors.initials: AB
authors.lastname: Díaz
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D006868:Hydrolysis; D018547:Plant Stems / Q000737:chemistry; D011096:Polygalacturonase / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry*; D014866:Waste Products
chemical_list: D011134:Polysaccharides; D013464:Sulfuric Acids; D014866:Waste Products; D012972:Sodium Hydroxide; D011096:Polygalacturonase; C033158:sulfuric acid
keywords: Acid pretreatment; Agricultural residue; Alkaline pretreatment; Enzymatic hydrolysis; Soybean straw