title: IL-10 gene polymorphism and influence of chemotherapy on cytokine plasma levels in childhood acute lymphoblastic leukemia patients: IL-10 polymorphism and plasma levels in leukemia patients.
abstract: Acute Lymphoblastic Leukemia is the leading form of cancer in infancy, and compelling evidences suggest an involvement of altered immune competence on this malignancy pathogenesis. Interleukin 10 (IL-10) is a pleiotropic cytokine designated as an immunosuppressive molecule, but may act as an immunostimulant factor in cancer development and progression. An IL-10 single nucleotide polymorphism (SNP) rs1800896 has been associated with disease progression to ALL, and might influence cytokine expression. This study analyzed the IL-10 rs1800896 polymorphism and performed a case-control study to determine the significant associations with ALL susceptibility and prognosis. IL-10 plasma levels were determined and associated with genotypes and disease phase. The study consisted of 67 childhood ALL patients and 75 age-related healthy controls. The rs1800896 was not associated with ALL susceptibility or risk of relapse. No significant association was observed between different genotypes of the rs1800896 and plasma levels of IL-10. Cytokine plasma levels were significantly higher in the diagnosis group (9.71 pg/mL ± 3.7), comparing to the treatment (3.48 pg/mL ± 1.3; p=0.01) and remission phase (0.12 pg/mL ± 0.1; p=0.0001) groups. This work indicates that the IL-10 plasma expression is altered from ALL disease diagnosis and remission. Moreover, prospective studies will establish the functional role of IL-10 in immune modulation in childhood ALL.
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Carlos Hiroji
authors.initials: CH
authors.lastname: Hiroki
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Marla Karine
authors.initials: MK
authors.lastname: Amarante
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Diego Lima
authors.initials: DL
authors.lastname: Petenuci
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Alberto Yoichi
authors.initials: AY
authors.lastname: Sakaguchi
authors.affiliation: Londrina Cancer Hospital and University Hospital of State University of Londrina, Medical Clinic Department, Londrina, PR, Brazil.
authors.forename: Fausto Celso
authors.initials: FC
authors.lastname: Trigo
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Maria Angelica Ehara
authors.initials: MA
authors.lastname: Watanabe
authors.affiliation: Laboratory of Study and Application of DNA Polymorphisms, Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil. Electronic address: kaducoral@gmail.com.
authors.forename: Carlos Eduardo Coral
authors.initials: CE
authors.lastname: de Oliveira
mesh_terms: D000483:Alleles; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics*; D008297:Male; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000188:drug therapy / Q000235:genetics*
chemical_list: D016753:Interleukin-10
keywords: Acute Lymphoblastic Leukemia; IL-10; Plasma; Polymorphism; Prognosis