title: Cultivation of Nannochloropsis for eicosapentaenoic acid production in wastewaters of pulp and paper industry.
abstract: The eicosapentaenoic acid (EPA) containing marine microalga Nannochloropsis oculata was grown in an effluent from anaerobic digestion of excess activated sludge from a wastewater treatment plant serving a combination of a pulp and a paper mill and a municipality (digester effluent, DE), mixed with the effluent of the same wastewater treatment plant. The maximum specific growth rate and photosynthesis of N. oculata were similar in the DE medium and in artificial sea water medium (ASW) but after 7 days, algae grown in the DE medium contained seven times more triacylglycerols (TAGs) per cell than cells grown in ASW, indicating mild stress in the DE medium. However, the volumetric rate of EPA production was similar in the ASW and DE media. The results suggest that N. oculata could be used to produce EPA, utilizing the nutrients available after anaerobic digestion of excess activated sludge of a pulp and paper mill.
authors.affiliation: Department of Biochemistry/Molecular Plant Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Anna
authors.initials: A
authors.lastname: Polishchuk
authors.affiliation: Department of Biochemistry/Molecular Plant Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Valev
authors.affiliation: Department of Biochemistry/Food Chemistry and Food Development, University of Turku, 20014 Turku, Finland.
authors.forename: Marko
authors.initials: M
authors.lastname: Tarvainen
authors.affiliation: Department of Biochemistry/Molecular Plant Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Sujata
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, P.O. Box 541, 33101 Tampere, Finland.
authors.forename: Viljami
authors.initials: V
authors.lastname: Kinnunen
authors.affiliation: Department of Biochemistry/Molecular Plant Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Taras
authors.initials: T
authors.lastname: Antal
authors.affiliation: Department of Biochemistry/Food Chemistry and Food Development, University of Turku, 20014 Turku, Finland.
authors.forename: Baoru
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, P.O. Box 541, 33101 Tampere, Finland.
authors.forename: Jukka
authors.initials: J
authors.lastname: Rintala
authors.affiliation: Department of Biochemistry/Molecular Plant Biology, University of Turku, 20014 Turku, Finland.
authors.forename: Esa
authors.initials: E
authors.lastname: Tyystjärvi
mesh_terms: D001673:Biodegradation, Environmental; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D007220:Industrial Waste / Q000032:analysis*; D019216:Metals, Heavy / Q000032:analysis; D010209:Paper; D058433:Photobioreactors / Q000382:microbiology; D010788:Photosynthesis; D012623:Seawater; D058009:Stramenopiles / Q000254:growth & development* / Q000378:metabolism; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*
chemical_list: D015118:Eicosapentaenoic Acid; D007220:Industrial Waste; D019216:Metals, Heavy; D062065:Wastewater
keywords: Eicosapentaenoic acid; Nannochloropsis; Paper mill; Pulp mill; Wastewater