title: Selective Involvement of the Checkpoint Regulator VISTA in Suppression of B-Cell, but Not T-Cell, Responsiveness by Monocytic Myeloid-Derived Suppressor Cells from Mice Infected with an Immunodeficiency-Causing Retrovirus.
abstract: Inhibition of T-cell responses in tumor microenvironments by myeloid-derived suppressor cells (MDSCs) is widely accepted. We demonstrated augmentation of monocytic MDSCs whose suppression of not only T-cell, but also B-cell, responsiveness paralleled the immunodeficiency during LP-BM5 retrovirus infection. MDSCs inhibited T cells by inducible nitric oxide synthase (iNOS)/nitric oxide (NO), but uniquely, inhibition of B cells was ~50% dependent each on iNOS/NO and the MDSC-expressed negative-checkpoint regulator VISTA. Blockade with a combination of iNOS/NO and VISTA caused additive or synergistic abrogation of MDSC-mediated suppression of B-cell responsiveness.
authors.affiliation: Geisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, and Norris Cotton Cancer Center, Lebanon, New Hampshire, USA kathy.a.green@Dartmouth.edu william.r.green@Dartmouth.edu.
authors.forename: Kathy A
authors.identifier: http://orcid.org/0000-0002-0681-2937
authors.initials: KA
authors.lastname: Green
authors.affiliation: Geisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, and Norris Cotton Cancer Center, Lebanon, New Hampshire, USA Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, Milwaukee, Wisconsin, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Geisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, and Norris Cotton Cancer Center, Lebanon, New Hampshire, USA Department of Nephrology and Transplantation, Medical Research Council Centre for Transplantation, King's College London Guy's Hospital, London, United Kingdom.
authors.forename: Randolph J
authors.initials: RJ
authors.lastname: Noelle
authors.affiliation: Geisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, and Norris Cotton Cancer Center, Lebanon, New Hampshire, USA kathy.a.green@Dartmouth.edu william.r.green@Dartmouth.edu.
authors.forename: William R
authors.initials: WR
authors.lastname: Green
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000276:immunology* / Q000473:pathology; D009569:Nitric Oxide / Q000235:genetics / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D012192:Retroviridae Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
chemical_list: D008565:Membrane Proteins; C548983:Vsir protein, mouse; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse