title: Berberine Prolongs Life Span and Stimulates Locomotor Activity ofDrosophila melanogaster.
abstract: Drosophila melanogastermutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KYN metabolism, prolonged life span in wild-type flies. Both inhibitors are not available for human use. Berberine, an isoquinoline alkaloid isolated fromBerberis aristata, is known as the herb widely used in traditional Chinese and Indian medicine. Berberin is a strong inhibitor of the enzyme catalyzing TRP conversion into KYN. Considering this particular feature we investigated the effect of berberine on life- and health-span in wild-typeDrosophila melanogaster. The results of our study showed that Berberine extended mean, median and maximum life span of female flies. Berberine did not affect the number of pupae of filial generation and decreased their lethality. Berberine increased locomotor activity (vertical climbing). The results of the study suggest that berberine prolongs life- and improves health-span ofDrosophila melanogaster. Berberine might be a candidate drug for prevention and treatment of aging and aging-associated medical and psychiatric disorders.
authors.affiliation: V. N. Karazin Kharkiv National University, Kharkiv, Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Navrotskaya
authors.affiliation: Tufts University, Tufts Medical Center, Boston, USA.
authors.forename: G
authors.initials: G
authors.lastname: Oxenkrug
authors.affiliation: V. N. Karazin Kharkiv National University, Kharkiv, Ukraine.
authors.forename: L I
authors.initials: LI
authors.lastname: Vorobyova
authors.affiliation: Tufts University, Tufts Medical Center, Boston, USA.
authors.forename: P
authors.initials: P
authors.lastname: Summergrad
keywords: Berberine; Drosophila melanogaster; Kynurenine; Life Span; Vertical Climbing; Viability