title: The Application of Micropipette Aspiration in Molecular Mechanics of Single Cells.
abstract: Micropipette aspiration is arguably the most classical technique in mechanical measurements and manipulations of single cells. Despite its simplicity, micropipette aspiration has been applied to a variety of experimental systems that span different length scales to study cell mechanics, nanoscale molecular mechanisms in single cells, bleb growth, and nucleus dynamics, to name a few. Enabled by micro/nanotechnology, several novel microfluidic devices have been developed recently with better accuracy, sensitivity, and throughput. Further technical advancements of microfluidics-based micropipette aspiration would have broad applications in both fundamental cell mechanics studies and for disease diagnostics.
authors.affiliation: Department of Mechanical Engineering, University of Michigan , 2350 Hayward Street , Ann Arbor, MI 48109-2125 e-mail:  melmlee@umich.edu.
authors.forename: Lap Man
authors.initials: LM
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Biomedical Engineering, Cell and Molecular Biology Program, Biophysics Program, University of Michigan , 2350 Hayward Street , Ann Arbor, MI 48109-2125 e-mail:  allenliu@umich.edu.
authors.forename: Allen P
authors.initials: AP
authors.lastname: Liu