title: Immunoblotting Using Radiolabeled Reagents for Detection.
abstract: Development of immunoblots is commonly performed using enzyme-labeled antibodies which convert soluble substrates into insoluble colored products. A simple, rapid, and sensitive alternative method which produces low background and allows a rapid quantitative evaluation is the use of radiolabeled antibodies or protein A conjugates. Here we describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification.
authors.affiliation: Institut für Immunologie, Carl Gustav Carus Technische Universität Dresden, Germany, Fetscherstrasse 74, 01307, Dresden, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Bartsch
authors.forename: Claudia
authors.initials: C
authors.lastname: Franke
authors.forename: Michael P
authors.initials: MP
authors.lastname: Bachmann
mesh_terms: D001324:Autoantigens / Q000032:analysis* / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D054879:Halogenation; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D007457:Iodine Radioisotopes / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000032:analysis* / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000737:chemistry; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D018796:Immunoconjugates; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D012261:Ribonucleoproteins; D013205:Staphylococcal Protein A; D000097585:SS-B Antigen