title: Entomotoxic properties of Dioclea violacea lectin and its effects on digestive enzymes of Anagasta kuehniella (Lepidoptera).
abstract: Entomotoxic plant lectins have been extensively studied in the past two decades, yet the exact mechanisms underlying their toxic effects remain unknown. This study investigated the effects of Dioclea violacea lectin (DVL) on larval development in Anagasta kuehniella. Chronic exposure of larvae (from neonates to the fourth instar) demonstrated that DVL interfered with larval growth, retarding development and decreasing larval mass without affecting survival. DVL decreased trypsin-like, chymotrypsin-like, and α-amylase activities and proved resistant to proteolysis by midgut proteases up to 24h. Shorter exposures to dietary DVL had no effect on midgut enzyme activity. Feeding fourth-instar larvae with fluorescently-labeled DVL revealed lectin binding to the peritrophic membrane.
authors.affiliation: Programa de Pós-Graduação em Ecologia e Conservação, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil; Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular, SBBq, Brazil.
authors.forename: Carolina Turatti
authors.initials: CT
authors.lastname: Oliveira
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, C.P. 476, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Daniele
authors.initials: D
authors.lastname: Kunz
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, C.P. 476, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Carlos Peres
authors.initials: CP
authors.lastname: Silva
authors.affiliation: Programa de Pós-Graduação em Ecologia e Conservação, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil; Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular, SBBq, Brazil; Unidade de Tecnologia de Alimentos e Saúde Pública, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, C.P. 549, 79070-900 Campo Grande, MS, Brazil. Electronic address: bioplant@terra.com.br.
authors.forename: Maria Lígia Rodrigues
authors.initials: ML
authors.lastname: Macedo
mesh_terms: D000818:Animals; D001835:Body Weight; D052598:Dioclea; D041981:Gastrointestinal Tract / Q000201:enzymology; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D009036:Moths / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D037121:Plant Lectins / Q000378:metabolism* / Q000633:toxicity; D059748:Proteolysis
chemical_list: D037121:Plant Lectins
keywords: Anagasta kuehniella; Bioinsecticides; Dioclea violacea; Enzyme activity; Insect gut; Lectin