title: Whole-genome expression analysis of Rice black-streaked dwarf virus in different plant hosts and small brown planthopper.
abstract: Rice black-streaked dwarf virus (RBSDV) can infect a number of gramineous plants and cause severe crop yield losses in southeast Asian countries. The virus is transmitted by small brown planthopper (SBPH) in a persistent circulative manner. The interactions between RBSDV and its different hosts remain unknown. Besides, how the virus adjusts itself to infect different hosts is unclear. In the present study, the relative RNA levels of the thirteen RBSDV genes in rice, maize, wheat, and SBPH were measured by real-time quantitative PCR. P7-1 and P10 genes were predominantly expressed whereas P8 and P7-2 genes were expressed at low levels in plant hosts. Similar to the expression in rice, P7-1 was the most abundantly expressed gene and P8 was expressed at the lowest level in SBPH, indicating that RBSDV adopts the same strategy to infect distinct hosts. The high expression levels of the P7-1 gene in both plants and insect suggest that it can be used as the target gene for disease diagnostics. However, the expression levels of some genes varied from host to host. P5-1, P6 and P9-1, the components of the RBSDV viroplasm, are differentially expressed in different hosts. Moreover, western blot analysis showed that the quantity of the P9-1 protein was more abundant in SBPH than in plant hosts. These data indicate that the virus may adjust its own gene expression to replicate in different hosts. Analysis of time course of gene expression revealed that P7-1 stands out as the only gene highly expressed at the earliest time point and its expression precedes all others throughout infection from 8 to 24days post-inoculation. The high expression levels of the P7-1 gene suggest that it plays a significant role in RBSDV-host interactions.
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China. Electronic address: xuqiufang@jaas.ac.cn.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Ni
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ren
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China. Electronic address: yjzhou@jaas.ac.cn.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D006430:Hemiptera / Q000821:virology*; D058507:Host Specificity; D012275:Oryza / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D012087:Reoviridae / Q000235:genetics* / Q000502:physiology; D014908:Triticum / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000821:virology*
chemical_list: D014764:Viral Proteins
keywords: Gene expression; Insect; Plant; Real-time quantitative PCR; Rice black-streaked dwarf virus