title: Evaluation of a Commercial ELISA for the Detection of Antibodies to Sarcoptes scabiei in Wild Boar (Sus scrofa).
abstract: Sarcoptic mange occurs in free-ranging wild boar (Sus scrofa) but has been poorly described in this species. We evaluated the performance of a commercial indirect enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of sarcoptic mange in domestic swine when applied to wild boar sera. We tested 96 sera from wild boar in populations without mange history ("truly noninfected") collected in Switzerland between December 2012 and February 2014, and 141 sera from free-ranging wild boar presenting mange-like lesions, including 50 live animals captured and sampled multiple times in France between May and August 2006 and three cases submitted to necropsy in Switzerland between April 2010 and February 2014. Mite infestation was confirmed by skin scraping in 20 of them ("truly infected"). We defined sensitivity of the test as the proportion of truly infected that were found ELISA-positive, and specificity as the proportion of truly noninfected that were found negative. Sensitivity and specificity were 75% and 80%, respectively. Success of antibody detection increased with the chronicity of lesions, and seroconversion was documented in 19 of 27 wild boar sampled multiple times that were initially negative or doubtful. In conclusion, the evaluated ELISA has been successfully applied to wild boar sera. It appears to be unreliable for early detection in individual animals but may represent a useful tool for population surveys.
authors.affiliation: 1  Centre for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, Postfach 8466, 3001 Bern, Switzerland.
authors.forename: Chloé
authors.initials: C
authors.lastname: Haas
authors.affiliation: 2  Office National de la Chasse et de la Faune Sauvage, Unité Sanitaire de la Faune, Micropolis, la Bérardie, Belle Aureille, 05000 Gap, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Rossi
authors.affiliation: 1  Centre for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, Postfach 8466, 3001 Bern, Switzerland.
authors.forename: Roman
authors.initials: R
authors.lastname: Meier
authors.affiliation: 1  Centre for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, Postfach 8466, 3001 Bern, Switzerland.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Ryser-Degiorgis
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000276:immunology / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D008297:Male; D012520:Sarcoptes scabiei / Q000276:immunology*; D012532:Scabies / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D034421:Sus scrofa / Q000276:immunology / Q000469:parasitology*; D013557:Switzerland
keywords: ELISA; Sarcoptes scabiei; evaluation; sarcoptic mange; seroconversion; wild boar