title: Nitrate removal by organotrophic anaerobic ammonium oxidizing bacteria with C2/C3 fatty acid in upflow anaerobic sludge blanket reactors.
abstract: In anaerobic ammonium oxidation (Anammox) process, a harsh ratio of nitrite to ammonia in influent was demanded, and the max nitrogen removal efficiency could only achieve to 89%, both of which limited the development of Anammox. The aim of this work was to study the nitrate removal by organotrophic anaerobic ammonium oxidizing bacteria (AAOB) with C2/C3 fatty acid in upflow anaerobic sludge blanket (UASB) reactors. In this study, organotrophic AAOB was successfully enriched by adding acetate and propionate with the total organic carbon to nitrogen (TOC/N) ratio of 0.1. In the condition of low substrate, the TN removal efficiency reached 90%, with the effluent TN of around 11.8 mg L(-1). After the addition of acetate and propionate, the predominant species in Anammox granular sludge transformed to Candidatus Jettenia that belonging to organotrophic AAOB from the Candidatus Kuenenia relating to general AAOB.
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: bjut_lidong@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D005227:Fatty Acids / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D009566:Nitrates / Q000302:isolation & purification*; D009584:Nitrogen / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification
chemical_list: D064751:Ammonium Compounds; D005227:Fatty Acids; D009566:Nitrates; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater
keywords: Granular sludge; High-throughput pyrosequencing; Nitrate removal; Organotrophic Anammox