title: Selenium supplementation induces mitochondrial biogenesis in trophoblasts.
abstract: BACKGROUND
  Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium.
  
  
  METHODS
  Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by western blotting.
  
  
  RESULTS
  Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.
  
  
  CONCLUSIONS
  These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts.
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Alisha
authors.initials: A
authors.lastname: Khera
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Lan-Feng
authors.initials: LF
authors.lastname: Dong
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia. Electronic address: o.holland@griffith.edu.au.
authors.forename: Olivia
authors.initials: O
authors.lastname: Holland
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Vanderlelie
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Elham A
authors.initials: EA
authors.lastname: Pasdar
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia; Institute of Biotechnology, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Neuzil
authors.affiliation: School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Anthony V
authors.initials: AV
authors.lastname: Perkins
mesh_terms: D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D051265:NF-E2-Related Factor 1 / Q000378:metabolism; D001678:Organelle Biogenesis; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage*; D051140:Selenoproteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004268:DNA-Binding Proteins; D051265:NF-E2-Related Factor 1; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species; D051140:Selenoproteins; D014157:Transcription Factors; C519118:selenoprotein H, human; D012643:Selenium
keywords: Antioxidant; Mitochondrial biogenesis; Reactive oxygen species; Selenium; Trophoblast