title: [Ultrasound molecular detection of immediately blood-mediated inflammatory reaction induced by  islets transplantation in vitro].
abstract: OBJECTIVE
  To study the feasibility of ultrasonic molecular imaging of immediately blood-mediated inflammatory reaction (IBMIR) in vitro.
  
  
  METHODS
  IBMIR models in vitro were divided into 3 groups: Group A, no microbubbles were added; Group B, non-targeted micro-bubbles were added; Group C, Lys-Gly-Asp-Ser (KGDS)-targeted microbubbles (MBK) were added. The ultrasonic enhancement of IBMIR in loops by ultrasonic contrast imaging was evaluated.
  
  
  RESULTS
  The contrast-enhanced US imaging did not show thrombus formation in the group A, whereas the thrombus was found in the Group B and C with a change in filling defects or ring enhancement, respectively. The time for detecting thrombosis was (7.3 ± 0.5) min and (13.2 ± 0.6) min in Group B and Group C, respectively (P<0.05). The average-gray scales of thrombus in Group B and Group C were 31.22 ± 3.56 and 75.85 ± 5.21, respectively (P<0.05). The fluorescence microscope also showed that MBK was attached to thrombus surrounding islets.
  
  
  CONCLUSIONS
  IBMIR model in vitro showed that KGDS-targeted ultrasound contrast agent could adhere to thrombus shell surrounding islets and molecular target ultrasonography could image these thrombi noninvasively and effectively.
authors.affiliation: Power Metallurgy Research Institute, Central South University, Changsha 410083; Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Ma
authors.affiliation: Institute of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D003287:Contrast Media; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging*; D016381:Islets of Langerhans Transplantation; D045423:Microbubbles; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014463:Ultrasonography
chemical_list: D003287:Contrast Media