title: Dual-Color Monitoring Overcomes the Limitations of Single Bioluminescent Reporters in Fast-Growing Microbes and Reveals Phase-Dependent Protein Productivity during the Metabolic Rhythms of Saccharomyces cerevisiae.
abstract: Luciferase is a useful, noninvasive reporter of gene regulation that can be continuously monitored over long periods of time; however, its use is problematic in fast-growing microbes like bacteria and yeast because rapidly changing cell numbers and metabolic states also influence bioluminescence, thereby confounding the reporter's signal. Here we show that these problems can be overcome in the budding yeast Saccharomyces cerevisiae by simultaneously monitoring bioluminescence from two different colors of beetle luciferase, where one color (green) reports activity of a gene of interest, while a second color (red) is stably expressed and used to continuously normalize green bioluminescence for fluctuations in signal intensity that are unrelated to gene regulation. We use this dual-luciferase strategy in conjunction with a light-inducible promoter system to test whether different phases of yeast respiratory oscillations are more suitable for heterologous protein production than others. By using pulses of light to activate production of a green luciferase while normalizing signal variation to a red luciferase, we show that the early reductive phase of the yeast metabolic cycle produces more luciferase than other phases.
authors.affiliation: Department of Biology, Middle Tennessee State University, Murfreesboro, Tennessee, USA.
authors.forename: Archana
authors.initials: A
authors.lastname: Krishnamoorthy
authors.affiliation: Department of Biology, Middle Tennessee State University, Murfreesboro, Tennessee, USA james.robertson@mtsu.edu.
authors.forename: J Brian
authors.initials: JB
authors.lastname: Robertson
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D002452:Cell Count; D003116:Color; D017930:Genes, Reporter; D008027:Light; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D008156:Luciferases