title: Role of microRNA-101a in the regulation of goat skeletal muscle satellite cell proliferation and differentiation.
abstract: MicroRNAs are a class of small, non-coding RNAs that participate in the regulation of diverse biological processes including skeletal muscle development. However, the underlying molecular mechanisms are not fully understood, particularly in goat. Here, we identified goat miR-101a as a novel myogenic microRNA mediating myogenic differentiation. The expression of miR-101a was enriched in goat skeletal muscles and up-regulated during satellite cell differentiation. After transfection with a miR-101a mimic and culturing in differentiation medium, satellite cell differentiation was promoted, accompanied by a significant increase in mRNA expression of the myogenic marker, MyoG, and decreased expression of MyoD. In contrast, blocking the function of miR-101a with a 2'-O-methylated antisense oligonucleotide inhibitor repressed satellite cell differentiation. However, both gain- and loss-of-function studies demonstrated that miR-101a had no significant influence on satellite cell proliferation. Therefore, our results provide a new insight on miR-101a in the regulation of goat skeletal muscle satellite cell proliferation and differentiation.
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Zhan
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Linjie
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Herbivorous, Sichuan Province Academy of Animal Husbandry, Chengdu, China.
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Fan
authors.affiliation: Institute of Herbivorous, Sichuan Province Academy of Animal Husbandry, Chengdu, China.
authors.forename: Chaorui
authors.initials: C
authors.lastname: Xiong
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China. Electronic address: zhp@sicau.edu.cn.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006041:Goats / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D018482:Muscle, Skeletal / Q000502:physiology*; D017570:MyoD Protein / Q000235:genetics*; D018008:Myogenin / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D017570:MyoD Protein; D018008:Myogenin; D016376:Oligonucleotides, Antisense
keywords: Goat; MicroRNA; Myoblast differentiation; Myogenesis; miR-101a