title: The detection of Dichelobacter nodosus and Fusobacterium necrophorum from ovine footrot in Kashmir, India.
abstract: In a study conducted, a total of 450 swab samples from footrot lesions of naturally infected sheep were collected in all the ten districts of the Kashmir valley and were examined for the presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum), in order to determine if F. necrophorum was associated with ovine footrot. The detection of F. necrophorum and D. nodosus was carried out by polymerase chain reaction targeting the leukotoxin (lktA) and 16S rRNA genes, respectively. In this study, only less than 50% of positive samples contained both the bacteria, so it is not possible to conclude with certainty that both bacteria are together required for the disease manifestation.
authors.affiliation: KVK-Kupwara, Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST) of Kashmir, Jammu and Kashmir, India. Electronic address: shaheen_mvsc@yahoo.co.in.
authors.forename: Shaheen
authors.initials: S
authors.lastname: Farooq
authors.affiliation: Division of Veterinary Microbiology & Immunology, SKUAST of Kashmir, Shuhama (Alusteng), Jammu and Kashmir, India.
authors.forename: Shakil A
authors.initials: SA
authors.lastname: Wani
authors.affiliation: Department of Sheep Husbandry, Government of Jammu and Kashmir, India.
authors.forename: Mir Nadeem
authors.initials: MN
authors.lastname: Hassan
authors.affiliation: SKUAST of Kashmir, Shuhama (Alusteng), Jammu and Kashmir, India.
authors.forename: Nazima
authors.initials: N
authors.lastname: Nazir
authors.affiliation: Division of Veterinary Microbiology & Immunology, SKUAST of Kashmir, Shuhama (Alusteng), Jammu and Kashmir, India.
authors.forename: Qazi Javed
authors.initials: QJ
authors.lastname: Nyrah
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000235:genetics; D020376:Dichelobacter nodosus / Q000302:isolation & purification*; D005098:Exotoxins / Q000235:genetics; D005534:Foot Diseases / Q000382:microbiology / Q000662:veterinary*; D005675:Fusobacterium necrophorum / Q000302:isolation & purification*; D007194:India; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005098:Exotoxins; D012336:RNA, Ribosomal, 16S; C018579:leukotoxin
keywords: Dichelobacter nodosus; Fusobacterium necrophorum; Ovine footrot; PCR