title: Control of oxygen release from peroxides using polymers.
abstract: An important limitation in cell therapy for the regeneration of tissue is the initial lack of oxygen. After implantation of large 3D cell-seeded structures, cells die rather than contribute to tissue regenerating. Here we've tested oxygen-releasing materials to improve cell survival and growth after implantation. Calcium peroxide (CaO2) in a polymer matrix was used as source of oxygen. Two polymers were tested in order to slow down and extend the period of oxygen release, poly(D,L-lactic acid) and poly(lactic-co-glycolic acid). Compared to CaO2 particles, both releasing systems showed an initially higher and shorter oxygen release. Human mesenchymal stromal cells cultured on casted films of these oxygen-releasing composites required catalase to proliferate, indicating the production of cytotoxic hydrogen peroxide as intermediate. Poly(D,L-lactic acid) and poly(lactic-co-glycolic acid) are less suited for slowly oxygen-releasing materials. Catalase was able to reduce the cytotoxic effect of H2O2.
authors.affiliation: Department of Biomedical Engineering (FB40), University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
authors.forename: Hilde
authors.initials: H
authors.lastname: Steg
authors.forename: Arina T
authors.initials: AT
authors.lastname: Buizer
authors.forename: Willem
authors.initials: W
authors.lastname: Woudstra
authors.forename: Albert G
authors.initials: AG
authors.lastname: Veldhuizen
authors.forename: Sjoerd K
authors.initials: SK
authors.lastname: Bulstra
authors.forename: Dirk W
authors.initials: DW
authors.lastname: Grijpma
authors.forename: Roel
authors.initials: R
authors.lastname: Kuijer
mesh_terms: D010100:Oxygen / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D010545:Peroxides; D011108:Polymers; D010100:Oxygen