title: Mucosal Alpha-Papillomavirus (HPV89) in a rare skin lesion.
abstract: BACKGROUND
  Apocrine acrosyringeal keratosis is a rare skin lesion showing a unique benign keratotic lesion associated with syringocystoadenoma papilliferum. It is characterized by an exophytic proliferation of the epidermis with two distinct keratinocytic structures: a) columns of hyperkeratotic mass surrounded by acanthotic epidermis and b) papillated and/or cystic invaginations typical of syringocystoadenoma papilliferum. No causative agents were reported.
  
  
  RESULTS
  The present report describes a typical case of apocrine acrosyringeal keratosis localized in the right retro-auricular area of 57-year-old man in which the presence of HPV was evaluated. PCR analysis and direct sequencing revealed the presence of HPV 89. The presence of this low risk mucosal HPV in a skin localization was never reported as well as in association with this rare tumor. Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.
  
  
  CONCLUSIONS
  Taken together our results suggest that HPV89 plays a role in apocrine acrosyringeal keratosis with syringocystoadenoma papilliferum, in consideration of the documented biological activity of the virus. The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management. Further studies on samples from other patients are needed to confirm this association.
authors.affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. paolinifrancesca@hotmail.com.
authors.forename: Francesca
authors.initials: F
authors.lastname: Paolini
authors.affiliation: Laboratory of Cutaneous Histopathology, San Gallicano Dermatologic Institute, Rome, Italy. cota@ifo.it.
authors.forename: Carlo
authors.initials: C
authors.lastname: Cota
authors.affiliation: Laboratory of Cutaneous Histopathology, San Gallicano Dermatologic Institute, Rome, Italy. adamantea@ifo.it.
authors.forename: Ada
authors.initials: A
authors.lastname: Amantea
authors.affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. gianfrancacurzio@gmail.com.
authors.forename: Gianfranca
authors.initials: G
authors.lastname: Curzio
authors.affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. venuti@ifo.it.
authors.forename: Aldo
authors.initials: A
authors.lastname: Venuti
mesh_terms: D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D007642:Keratosis / Q000175:diagnosis* / Q000473:pathology* / Q000821:virology; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000473:pathology* / Q000821:virology; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017193:Skin Diseases, Viral / Q000175:diagnosis* / Q000473:pathology* / Q000821:virology
chemical_list: D004279:DNA, Viral