title: Enhancing biological analyses with three dimensional field asymmetric ion mobility, low field drift tube ion mobility and mass spectrometry (μFAIMS/IMS-MS) separations.
abstract: Multidimensional high throughput separations are ideal for analyzing distinct ion characteristics simultaneously in one analysis. We report on the first evaluation of a platform coupling a high speed field asymmetric ion mobility spectrometry microchip (μFAIMS) with drift tube ion mobility and mass spectrometry (IMS-MS). The μFAIMS/IMS-MS platform was used to analyze biological samples and simultaneously acquire multidimensional FAIMS compensation fields, IMS drift times, and accurate ion masses for the detected features. These separations thereby increased the overall measurement separation power, resulting in greater information content and more complete characterization of the complex samples. The separation conditions were optimized for sensitivity and resolving power by the selection of gas compositions and pressures in the FAIMS and IMS separation stages. The resulting performance provided three dimensional separations, benefitting both broad complex mixture studies and targeted analyses by improving isomeric separations and allowing detection of species obscured by interfering peaks.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. erin.baker@pnnl.gov.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Yehia M
authors.initials: YM
authors.lastname: Ibrahim
authors.forename: Tsung-Chi
authors.initials: TC
authors.lastname: Chen
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Kyle
authors.forename: Randolph V
authors.initials: RV
authors.lastname: Norheim
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Monroe
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
mesh_terms: D000818:Animals; D002417:Cattle; D013058:Mass Spectrometry / Q000379:methods*; D009038:Motion; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D059748:Proteolysis; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; D014357:Trypsin