title: Metabolism of Chuanxiong Rhizoma decoction: Identification of the metabolites in WZS-miniature pig urine.
abstract: Chuanxiong Rhizoma (CR), a well-known traditional Chinese medicine originated from the rhizome of Ligusticum chuanxiong Hort., was effective for treating various vascular diseases. To identify the metabolites of CR in vivo, the drug-containing urine samples of WZS-miniature pigs after orally administrated CR decoction were collected, after sequential column chromatography 17 metabolites (M1-M17) were isolated from the methanol extract of the urine samples. Their structures, including nine phthalides (M1-M9) and eight phenolic acids (M10-M17), were identified by spectroscopic means. Among them, 8 were new ones (M1-M6, M11-M12). On the basis of the structures of identified metabolites, seven original constituents, including 2 phthalides (senkyunolideI/H) and 5 phenolic acids (ferulic acid, isoferulic acid, caffeic acid, 3-hydroxycinnamoyl acid and 4-hydroxybenzonic acid) were deduced to be the major absorbed original constituents of CR in vivo. This is the first study on the metabolites of CR decoction in non-rodent animal (WZS-miniature pig), the results will give an insight into the metabolism profiles of phthalides and phenolic acids in CR decoction in vivo.
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China. Electronic address: ydhui@bjmu.edu.cn.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Yang
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Qi-Le
authors.initials: QL
authors.lastname: Zhou
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, PR China. Electronic address: xwyang@bjmu.edu.cn.
authors.forename: Xiu-Wei
authors.initials: XW
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001572:Benzofurans / Q000652:urine; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000652:urine; D030004:Ligusticum / Q000737:chemistry*; D008297:Male; D015394:Molecular Structure; D027343:Rhizome / Q000737:chemistry*; D013552:Swine; D013556:Swine, Miniature; D016482:Urinalysis
chemical_list: D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C082022:phthalide; C017616:phenolic acid
keywords: Ligusticum chuanxiong; Metabolites in vivo; Phenolic acids; Phthalides; WZS-miniature pig