title: Retrieval of functional TCRs from single antigen-specific T cells: Toward individualized TCR-engineered therapies.
abstract: We have developed a highly versatile platform for the systematic retrieval of T-cell receptors (TCRs) from single-antigen-reactive T cells and for characterization of their function and specificity. This approach enables rapid extraction of multiple TCRs from repertoires in individuals and not only broadens the diversity of TCRs suitable for clinical use, but also sets the stage for actively personalized immunotherapeutic strategies.
authors.affiliation: TRON - Translational Oncology at the University Medical Center of Johannes Gutenberg University ; Mainz, Germany ; BioNTech Cell & Gene Therapies GmbH ; Mainz, Germany.
authors.forename: Tana
authors.initials: T
authors.lastname: Omokoko
authors.affiliation: TRON - Translational Oncology at the University Medical Center of Johannes Gutenberg University ; Mainz, Germany ; BioNTech Cell & Gene Therapies GmbH ; Mainz, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Simon
authors.affiliation: TRON - Translational Oncology at the University Medical Center of Johannes Gutenberg University ; Mainz, Germany.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Türeci
authors.affiliation: TRON - Translational Oncology at the University Medical Center of Johannes Gutenberg University ; Mainz, Germany ; BioNTech Cell & Gene Therapies GmbH ; Mainz, Germany ; Research Center for Immunotherapy (FZI) at the University Medical Hospital; Johannes Gutenberg-University ; Mainz, Germany.
authors.forename: Ugur
authors.initials: U
authors.lastname: Sahin
keywords:  adoptive cell transfer; T cell engineering; T cell receptor; epitope; personalized immunotherapy