title: Genetic polymorphisms of hspa1b and hspa1l in infertile men.
abstract: OBJECTIVE
  To investigate the relationship between the HSP 70 genepolymorphism and primary infertility in males with normal sperm-parameters.
  
  
  METHODS
  The case-control study was conducted in Sanliurfa, Turkey, from September 2010 to August 2011and comprised infertile males as cases and healthy fertile controls. Deoxyribonucleicacid was isolated from the blood of both groups, and polymorphisms of the HSPA1B gene (NM_005346.4, GI: 3304):c.1059G>A, (PstI G>A; dbSNP: rs1061581G>A) and HSPA1L gene (NM_005527.3, GI: 3305) c.1478C>T (NcoIC>T, dbSNP: rs2227956) were analysed with polymerase chain reaction-restriction fragment length polymorphism technique. SPSS version 11.5 was used for statistical analysis.
  
  
  RESULTS
  Of the 140males in the study, 68(28.5%) were infertile cases and 72(51.4%) fertile controls. There was no statistically significant difference between GA (heterozygous) and AA (homozygous, polymorphic) genotypes of the c.1059G>A polymorphic point of the HSPA1B gene or between the A allele of the cases and controls (p>0.05). There was no statistically significant difference between the CT (heterozygote) and TT (homozygous, polymorphic) genotypes of the c.1478C>T polymorphic area of the HSPA1Lgene or between the T alleles of the cases and the controls (p>0.05).
  
  
  CONCLUSIONS
  Infertility in males with normal sperm parameters was not significantly associated with HSPA1B:c.1059G>A and HSPA1L:c.1478C>T gene polymorphisms. Further prospective studies with larger sample sizes and different gene groups are required to clarify the issue.
authors.affiliation: Department of Urology, Harran University, Sanliurfa, Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Ciftci
authors.affiliation: Department of Urology, Harran University, Sanliurfa, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Celepkolo
authors.affiliation: Department of Medical Biology, Harran University, Sanliurfa, Turkey.
authors.forename: Fuat
authors.initials: F
authors.lastname: Dilmec
authors.affiliation: Department of Histology and Embryology, Harran University, Sanliurfa, Turkey.
authors.forename: Mete
authors.initials: M
authors.lastname: Köksal
authors.affiliation: Department of Urology, Harran University, Sanliurfa, Turkey.
authors.forename: Ercan
authors.initials: E
authors.lastname: Yeni
authors.affiliation: Department of Pathology, Erzurum Region Education and Research Hospital, Erzurum, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Yagmur
authors.affiliation: Department of Urology, Harran University, Sanliurfa, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Gümüs
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D055101:Semen Analysis; D014421:Turkey; D055815:Young Adult
chemical_list: D018840:HSP70 Heat-Shock Proteins; C525621:HSPA1B protein, human; C543454:HSPA1L protein, human
keywords: Infertility, HSP70, Gene, Polymorphism, PCR, RFLP.