title: Extended spectrum β-lactamase producing Escherichia coli in broiler breeding roosters: Presence in the reproductive tract and effect on sperm motility.
abstract: Extended spectrum β-lactamases (ESBL)-producing Escherichia coli have emerged worldwide in animal husbandry and they were reported from different ecosystems. The purpose of this study was firstly, to investigate the presence of ESBL-producing E. coli in the gastrointestinal (GIT) and reproductive (RT) tracts of broiler breeding roosters, and secondly to study the impact of an ESBL-producing E. coli on artificially infected semen. A total of seventeen ESBL-producing E. coli strains were isolated from the gastrointestinal and reproductive tracts of nine broiler breeding roosters. All isolates were identified to the species level by API 20E system and MALDI-TOF, serotyped, and genetically characterized for ESBL production. Semen was artificially infected with E. coli ATCC25922 or with an ESBL-producing E. coli strain recovered from the reproductive tract. A computer aided semen analyzer (CASA) was used to compare different spermatozoa motility parameters in each sample. All ESBL-producing E. coli isolates could not be typed with the currently used sera and they were harboring a blaCTX-M gene alone or in combination with a blaTEM gene. The semen quality was notably less affected in samples infected with ESBL-producing E. coli strain compared to the control and sample infected with E. coli ATCC25922. The present study revealed that ESBL-producing E. coli can be isolated from both reproductive and digestive tracts of broiler breeding roosters. Contamination of the reproductive tract with ESBL-producing E. coli could lead to contamination of semen and could be an important factor in the dissemination of ESBL-producing E. coli in poultry.
authors.affiliation: Laboratoire d'Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria. Electronic address: salima_mezhoud@yahoo.fr.
authors.forename: Halima
authors.initials: H
authors.lastname: Mezhoud
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Boyen
authors.affiliation: Laboratoire Associé en Ecosystèmes Marin et Aquacole, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Leg-Hel
authors.initials: LH
authors.lastname: Touazi
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Garmyn
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium; Tropical Veterinary Institute, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
authors.forename: Nassim
authors.initials: N
authors.lastname: Moula
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Annemieke
authors.initials: A
authors.lastname: Smet
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesbrouck
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Martel
authors.affiliation: Laboratoire Associé en Ecosystèmes Marin et Aquacole, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Mokrane
authors.initials: M
authors.lastname: Iguer-Ouada
authors.affiliation: Laboratoire d'Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Touati
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000150:complications / Q000662:veterinary*; D005837:Genitalia, Male / Q000382:microbiology; D008297:Male; D011201:Poultry Diseases / Q000382:microbiology*; D013081:Sperm Motility / Q000502:physiology; D018440:beta-Lactam Resistance
keywords: ESBL E. coli; Reproductive tract; Rooster; Semen motility