title: Integrated process of two stage cultivation of Nannochloropsis sp. for nutraceutically valuable eicosapentaenoic acid along with biodiesel.
abstract: The marine eustigmatophyte Nannochloropsis is one of the potential producers of eicosapentaenoic acid (EPA), a valued nutraceutical. Nannochloropsis sp. was cultivated under photoautotrophic condition utilizing CO2 in a two phase cultivation process in order to enhance the eicosapentaenoic acid (EPA) productivity. It was cultivated in a photobioreactor up to late log phase for cell growth (phase I). Then, the culture was harvested and confronted to relatively low temperature (10 °C) and low light (30 μmol photons m(-2) s(-1)) in both photobioreactor and Erlenmeyer flask (phase II), thus augmenting EPA% by 3.4 fold. Lower temperature with low light favored the synthesis of EPA although, biomass productivity, lipid content and lipid productivity were slightly decreased relative to phase I. The total lipids extracted from Nannochloropsis sp. fractionated into neutral lipids (NLs), glycolipids (GLs) and phospholipids (PLs) and a major proportion of EPA was found in phospholipids. Results suggested that low temperature and low light may ameliorate partitioning towards EPA in phospholipids.
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Madhusree
authors.initials: M
authors.lastname: Mitra
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Shailesh Kumar
authors.initials: SK
authors.lastname: Patidar
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000032:analysis; D019587:Dietary Supplements; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D009930:Organic Chemicals / Q000032:analysis; D058433:Photobioreactors / Q000382:microbiology; D010860:Pigments, Biological / Q000032:analysis; D058009:Stramenopiles / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D056804:Biofuels; D009930:Organic Chemicals; D010860:Pigments, Biological; D002244:Carbon; D015118:Eicosapentaenoic Acid
keywords: Eicosapentaenoic acid; Light intensity; Nannochloropsis sp.; Temperature; Two phase cultivation