title: Malathion-induced hepatotoxicity in male Wistar rats: biochemical and histopathological studies.
abstract: The increasing use of organophosphorus pesticides in the environment constitutes an ecotoxicological hazard especially for humans and non-target animals. Hereby, we analyzed the toxic effects of malathion on the histological structure of liver and biochemical parameters in male rats. Three groups received daily different amounts of malathion: 1/1000, 1/100, and 1/10 LD50 for 30 days. The weights of treated rat's liver have increased. Analyzed tissues showed centrilobular and sinusoidal congestion, hepatocyte hypertrophy, cellular vacuolization, anucleated hepatocytes, depletion of organelles affecting the majority of cells, and presence of necrotic foci into the hepatic parenchyma. Histological sections of the liver showed important hepatocyte glycogen storage. We conclude that malathion stimulates the filing of glycogen in a dose-dependent manner. Biochemical parameters showed that alanine transaminase (ALT), aspartate transaminase (AST), gamma glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) levels increased in the treated groups when the level of total protein decreased in intoxicated groups.
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Flehi-Slim
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia.
authors.forename: Issam
authors.initials: I
authors.lastname: Chargui
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia. sbgh@mail.com.
authors.forename: Sonia
authors.initials: S
authors.lastname: Boughattas
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia.
authors.forename: Aymen
authors.initials: A
authors.lastname: El Mabrouk
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Belaïd-Nouira
authors.affiliation: Laboratory of Biochemistry-Toxicology, University Hospital of Monastir, Monastir, Tunisia.
authors.forename: Fadwa
authors.initials: F
authors.lastname: Neffati
authors.affiliation: Laboratory of Biochemistry-Toxicology, University Hospital of Monastir, Monastir, Tunisia.
authors.forename: Mohamed Fadhel
authors.initials: MF
authors.lastname: Najjar
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia.
authors.forename: Zohra
authors.initials: Z
authors.lastname: Haouas
authors.affiliation: Laboratory of Histology and Cytogenetic, Faculty of Medicine, Research Unity of Genetic (02/UR/08-03), Avenue Ibnou Sina 5000, Monastir, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Ben Cheikh
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000209:etiology / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D006003:Glycogen / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008294:Malathion / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004785:Environmental Pollutants; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D008294:Malathion
keywords: Biochemical parameters; Gavage; Glycemia; Histopathology; Liver; Malathion; Rat