title: Quality control data of physiological and immunological biomarkers measured in serum and plasma.
abstract: In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years. The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples. Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values. A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up. Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.
authors.affiliation: Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: eugene.jansen@rivm.nl.
authors.forename: Eugène
authors.initials: E
authors.lastname: Jansen
authors.affiliation: Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: piet.beekhof@rivm.nl.
authors.forename: Piet
authors.initials: P
authors.lastname: Beekhof
authors.affiliation: Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: hans.cremers@rivm.nl.
authors.forename: Johannes
authors.initials: J
authors.lastname: Cremers
authors.affiliation: Leopold-Franzens-Universität Innsbruck, Institute for Biomedical Aging Research, Rennweg 10, 6020 Innsbruck, Austria. Electronic address: birgit.weinberger@uibk.ac.at.
authors.forename: Birgit
authors.initials: B
authors.lastname: Weinberger
authors.affiliation: Institute for Nutritional Sciences and Physiology, University for Health Sciences, Medical Informatics and Technology, Eduard Wallnoefer-Zentrum 1, 6060 Hall in Tirol, Austria. Electronic address: simone.fiegl@umit.at.
authors.forename: Simone
authors.initials: S
authors.lastname: Fiegl
authors.affiliation: Unit of Cellular Biochemistry and Biology, The University of Namur, Rue de Bruxelles 61, 5000 Namur, Belgium. Electronic address: olivier.toussaint@fundp.ac.be.
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
authors.affiliation: BioTeSys GmbH, Schelztorstraße 54-56, 73,728 Esslingen, Germany. Electronic address: j.bernhardt@biotesys.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bernhard
authors.affiliation: National Hellenic Research Foundation (NHRF) Institute of Biological Research and Biotechnology, 48 Vas. Constantinou Ave., Athens 11635, Greece. Electronic address: sgonos@eie.gr.
authors.forename: Efstathios
authors.initials: E
authors.lastname: Gonos
authors.affiliation: DIMES-Department of Experimental, Diagnostic and Specialty Medicine, CIG-Interdepartmentall Centre "L.Galvani", Alma Mater Studiorum, University of Bologna, 40,126 Bologna, Italy. Electronic address: miriam.capri@unibo.it.
authors.forename: Miriam
authors.initials: M
authors.lastname: Capri
authors.affiliation: DIMES-Department of Experimental, Diagnostic and Specialty Medicine, CIG-Interdepartmentall Centre "L.Galvani", Alma Mater Studiorum, University of Bologna, 40,126 Bologna, Italy. Electronic address: claudio.franceschi@unibo.it.
authors.forename: Claudio
authors.initials: C
authors.lastname: Franceschi
authors.affiliation: Nencki Institute of Experimental Biology, Polish Academy of Sciences, St. Pasteura 302-093 Warsaw, Poland. Electronic address: e.sikora@nencki.gov.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sikora
authors.affiliation: Molecular Toxicology, Department of Biology, Box 628, University of Konstanz, 78,457 Konstanz, Germany. Electronic address: maria.moreno-villanueva@uni-konstanz.de.
authors.forename: María
authors.initials: M
authors.lastname: Moreno-Villanueva
authors.affiliation: Institute of Nutritional Medicine (180c), University of Hohenheim, Fruwirthstraße 12, 70,599 Stuttgart, Germany. Electronic address: breusing@uni-hohenheim.de.
authors.forename: Nicolle
authors.initials: N
authors.lastname: Breusing
authors.affiliation: German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Arthur-Scheunert-Allee 114-116, 14,558 Nuthetal, Germany. Electronic address: scientific.director@dife.de.
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
authors.affiliation: Molecular Toxicology, Dept of Biology, Box 628, University of Konstanz, 78,457 Konstanz, Germany. Electronic address: alexander.buerkle@uni-konstanz.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Bürkle
authors.affiliation: Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: martijn.dolle@rivm.nl.
authors.forename: Martijn E T
authors.initials: ME
authors.lastname: Dollé
mesh_terms: D000375:Aging / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D000068598:Data Accuracy; D005260:Female; D006801:Humans; D008297:Male
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Hemolysis; Quality control