title: Regulation of type I-interferon responses in the human epidermal melanocyte cell line SKMEL infected by the Ross River alphavirus.
abstract: Melanocytes are melanin-producing cells and with emerging innate immune functions including the expression of antiviral interferon-type I cytokines. We herein ascertained the susceptibility of the human melanocytes to Ross River alphavirus (RRV) infection and analyzed the subsequent immune responses. We demonstrated for the first time that (1) SKMEL-28 melanocyte cell line was susceptible to RRV infection and displaying major cytopathic activities and (2) RRV interfered with the interferon-type I response by altering nuclear translocation of pSTAT1 and pSTAT2 in infected SKMEL-28. These results suggest that the human melanoma cell line SKMEL-28 is a valuable model to analyze the mechanisms involved in severe skin manifestations and melanocyte's immunity at the portal of entry of major infection by arboviruses.
authors.affiliation: UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Université de la Réunion, INSERM 1187, CNRS 9192, IRD 249, Reunion.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Assi
authors.affiliation: UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Université de la Réunion, INSERM 1187, CNRS 9192, IRD 249, Reunion; Biology Laboratory, CHU Félix Guyon, Reunion.
authors.forename: Vincent Gérard
authors.initials: VG
authors.lastname: Thon-Hon
authors.affiliation: Biology Laboratory, CHU Félix Guyon, Reunion.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Jaffar-Bandjee
authors.affiliation: UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Université de la Réunion, INSERM 1187, CNRS 9192, IRD 249, Reunion.
authors.forename: Audrey
authors.initials: A
authors.lastname: Martinez
authors.affiliation: UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Université de la Réunion, INSERM 1187, CNRS 9192, IRD 249, Reunion; Biology Laboratory, CHU Félix Guyon, Reunion. Electronic address: philippe.gasque@gmail.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: Gasque
mesh_terms: D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D008544:Melanocytes / Q000378:metabolism* / Q000821:virology; D012398:Ross River virus / Q000472:pathogenicity*
chemical_list: D007370:Interferon Type I
keywords: Arbovirus; Innate immunity; Interferon; Melanocyte