title: Neurokinin-2 receptor polymorphism predicts lymph node metastasis in colorectal cancer patients.
abstract: To analyze the single nucleotide polymorphisms (SNPs) of two subtypes of neurokinin (NK) receptors,NK1RandNK2R(also known asTAC1RandTAC2R), in colorectal cancer (CRC), peripheral blood samples were collected from 199 CRC patients. Direct-sequencing was performed to identify theNK1Rrs10198644 andNK2Rrs4644560 SNPs. Genotype results were correlated with clinical factors. The allele frequencies ofNK1Rrs10198644 GC, CC and GG were 52, 17 and 31%, respectively, while that ofNK2Rrs4644560 GC, CC, and GG were 36, 50 and 14%, respectively. Patients withNK2Rrs4644560 GC exhibited more positive lymph nodes than those with CC (mean, 2.2 vs. 1.3; P=0.016). Further analysis highlighted that the number of positive lymph nodes was also increased in theNK2Rrs4644560 GC/NK1Rrs10198644 GG group compared with theNK2Rrs4644560 GG/NK1Rrs10198644 GG group (mean, 2.2 vs. 0.9; P=0.04). These data suggested that theNK2Rrs4644560 GC polymorphism alone or combination withNK1Rrs10198644 GG may be a promising prognostic marker of lymph node metastasis in CRC patients.
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Fang
authors.affiliation: Lab of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, P.R. China ; Institute for Cell-based Drug Development of Zhejiang Province, Hangzhou, Zhejiang 310058, P.R. China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Fu
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China ; Institute for Cell-based Drug Development of Zhejiang Province, Hangzhou, Zhejiang 310058, P.R. China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Mou
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Fanlong
authors.initials: F
authors.lastname: Liu
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Qian
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Y I
authors.initials: YI
authors.lastname: Zheng
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Anorectum, People's Hospital of Shaoxing, Shaoxing, Zhejiang 312000, P.R. China.
authors.forename: Pingjiang
authors.initials: P
authors.lastname: Ye
authors.affiliation: Second People's Hospital of Jiaxing, Jiaxing, Zhejiang 314000, P.R. China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biotherapy Center, Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
keywords: colorectal cancer; neurokinin 2 receptor; positive lymph node; single nucleotide polymorphisms