title: MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice.
abstract: Skeletal muscle stem cells, called satellite cells, are a quiescent heterogeneous population. Their heterogeneity is influenced by Pax7, a well-defined transcriptional regulator of satellite cell functions that defines two subpopulations: Pax7(Hi) and Pax7(Lo). However, the mechanisms by which these subpopulations are established and maintained during myogenesis are not completely understood. Here we show that miR-431, which is predominantly expressed in the skeletal muscle, mediates satellite cell heterogeneity by fine-tuning Pax7 levels during muscle development and regeneration. In miR-431 transgenic mice, the Pax7(Lo) subpopulation is enriched, enhances myogenic differentiation and accelerates muscle regeneration. Notably, miR-431 attenuates the muscular dystrophic phenotype in mdx mice and may be a potential therapeutic target in muscular diseases. miR-431 transgenic mice are a unique genetic model for investigating the cellular features and biological functions of Pax7(Lo) satellite cells during muscle development and regeneration.
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Rimao
authors.initials: R
authors.lastname: Wu
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhai
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Zou
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Meng
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhong
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Changyin
authors.initials: C
authors.lastname: Li
authors.affiliation: Gladstone Institute of Cardiovascular Disease, Roddenberry Center for Stem Cell Biology and Medicine, 1650 Owens Street, San Francisco, California 94158, USA.
authors.forename: Haixia
authors.initials: H
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D051379:Mice; D018101:Mice, Inbred mdx; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000293:injuries / Q000473:pathology* / Q000502:physiology*; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology
chemical_list: C000599703:MIRN431 microRNA, mouse; D035683:MicroRNAs; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse