title: Chemerin inhibition of myogenesis and induction of adipogenesis in C2C12 myoblasts.
abstract: Chemerin is an adipocyte-secreted adipokine that regulates the differentiation and metabolism of adipose through auto-/paracrine signaling. Its function in the differentiation of multipotent myoblast cells has thus far received little attention. In this study, C2C12 myoblast cells were cultured in the medium with Chemerin, and the differentiation potential of C2C12 myoblasts was analyzed. The results showed that Chemerin increased ROS levels and TG content of C2C12 cells. At the same time, the mRNA expressions and protein concentrations of the adipogenic factors PPARγ, C/EBPα and UCP1 were up-regulated, while the muscle specific transcription factors MyoD, Myogenin and MyHC were decreased in cultured C2C12 cells. In conclusion, the adipokine Chemerin promoted the adipogenic differentiation potential and altered the fate of myoblast cells from myogenesis to adipogenesis, which contributed in part to the up-regulated adipocyte genes. Our study reveals the importance of functional Chemerin signaling in adipogenesis and in directing the differentiation of multipotent myoblast cells.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: lihuixia@njau.edu.cn.
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Kun-Lin
authors.initials: KL
authors.lastname: Chen
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Naesudong-ro, Seowon-gu, Cheongju-si 362-763, Chungcheongbuk-do, Republic of Korea.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Wang
authors.affiliation: National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China; Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chang-Bo
authors.initials: CB
authors.lastname: Tang
authors.affiliation: National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China; Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xing-Lian
authors.initials: XL
authors.lastname: Xu
authors.affiliation: National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China; Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: ghzhou@njau.edu.cn.
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Zhou
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D018925:Chemokines / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D051379:Mice; D024510:Muscle Development / Q000187:drug effects*; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; D017382:Reactive Oxygen Species; D014280:Triglycerides; C523686:chemerin protein, mouse
keywords: Adipogenesis; Brown adipose; C2C12 myoblast cells; Chemerin; Myogenesis