title: Characterization of 12 Novel Microsatellite Markers of Sogatella furcifera (Hemiptera: Delphacidae) Identified From Next-Generation Sequence Data.
abstract: The white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), is a major pest of rice and has long-range migratory behavior in Asia. Microsatellite markers (simple sequence repeats) have been widely used to determine the origins and genetic diversity of insect pests. We identified novel microsatellite loci for S. furcifera samples collected from Laos, Vietnam, and three localities in Bangladesh from next-generation Roche 454 pyrosequencing data. Size polymorphism at 12 microsatellite loci was verified for 40 adult individuals collected from Shinan, South Korea. The average number of alleles per locus was 7.92. The mean values of observed (H(o)) and expected heterozygosities (H(E)) were 0.615 and 0.757, respectively. These new microsatellite markers will be a resource for future ecological genetic studies of S. furcifera samples across more broad geographic regions in Asia and may assist in estimations of genetic differentiation and gene flow among populations for implementation of more effective management strategies to control this serious rice pest.
authors.affiliation: Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hwa Yeun
authors.initials: HY
authors.lastname: Nam
authors.affiliation: USDA-ARS, Corn Insects & Crop Genetics Research Unit, 113 Genetics Laboratory, Iowa State University, Ames, IA 50011.
authors.forename: Brad
authors.initials: B
authors.lastname: Coates
authors.affiliation: USDA-ARS, Corn Insects & Crop Genetics Research Unit, 113 Genetics Laboratory, Iowa State University, Ames, IA 50011.
authors.forename: Kyung Seok
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Marana
authors.initials: M
authors.lastname: Park
authors.affiliation: Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea jh7lee@snu.ac.kr.
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001210:Asia, Southeastern; D001459:Bangladesh; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D056910:Republic of Korea
keywords: Sogatella furcifera; genetic diversity; microsatellite marker