title: Hybrid, multiplexed, functional DNA nanotechnology for bioanalysis.
abstract: We herein aim to report on the fabrication of DNA nano-heterostructures usable as a robust multi-functional analytical system to obtain multiple and complex data in parallel format from a single sample with unprecedented analytical performances. The ability of chemical information contained in the sequences of programmed DNA structures to organize matter made DNA become a unique material in "the nanoworld". Such carefully designed DNA nanostructures can then be functionalized/templated with different biomolecules/nanomaterials as different as nanoparticles, nanowires, organic molecules, peptides, and proteins with controlled spacing on the nanometer scale (<10 nm). In this way, it is possible to combine the properties of both DNA and nanomaterials for exposing the designed functionality and customizable geometrical hetero-nanostructures. By coupling automated on-chip high yield DNA synthesis with low cost detection methods, DNA-nanotechnology can enable the realization of high-sensitivity, multiplexed bioanalytical assays for many different applications like diagnostics, drug screening, toxicology, immunology and biosensors.
authors.affiliation: Department of Chemical Science and Technologies & NAST Center, University of Rome Tor Vergata, via della Ricerca Scientifica 1, 00133 Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Arrabito
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D015374:Biosensing Techniques; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000737:chemistry / Q000378:metabolism; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D035683:MicroRNAs; D015341:Nucleic Acid Probes; D011506:Proteins; D004247:DNA