title: Structure activity characterization of Bordetella petrii lipid A, from environment to human isolates.
abstract: Bordetella petrii, a facultative anaerobic species, is the only known member of the Bordetella genus with environmental origin. However it was also recently isolated from humans. The structures of the B. petrii lipid A moieties of the endotoxins were characterized here for the first time for an environmental strain and compared to that of human isolates. Characterization was achieved using chemical analyses, gas chromatography-mass spectrometry, and Matrix Assisted Laser Desorption Ionisation mass spectrometry. The analyses revealed that the different lipid A structures contain a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide as well at the C-3' in ester linkages. Similar to Bordetella pertussis and Bordetella bronchiseptica lipids A, the hydroxytetradecanoic acid at the C-2' position was substituted by tetradecanoic acid. Unlike B. pertussis, the hydroxytetradecanoic acid at the C-2 position was substituted with either 12:0 or 14:0 and/or their 2-OH forms. Depending on the environmental or human origin the structures differed in the length and degree of fatty acid acylation and impacted the IL-6 and TNF-α inflammatory responses tested. In one isolate we showed the presence at the C-3 position of the short-chain 10:0(3-OH), which according to our previous analyses is more characteristic of the human pathogens in the genus like B. pertussis and Bordetella parapertussis.
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 409, 91405 Orsay, France.
authors.forename: Soorej M
authors.initials: SM
authors.lastname: Basheer
authors.affiliation: Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, URA CNRS 3012, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Bouchez
authors.affiliation: LPS-BioSciences, Bâtiment 409, Université de Paris-Sud, F-91405 Orsay, France.
authors.forename: Alexey
authors.initials: A
authors.lastname: Novikov
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 409, 91405 Orsay, France.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Augusto
authors.affiliation: Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, URA CNRS 3012, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Bâtiment 409, 91405 Orsay, France; LPS-BioSciences, Bâtiment 409, Université de Paris-Sud, F-91405 Orsay, France. Electronic address: martine.caroff@u-psud.fr.
authors.forename: Martine
authors.initials: M
authors.lastname: Caroff
mesh_terms: D001884:Bordetella / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008050:Lipid A / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D008297:Male; D013058:Mass Spectrometry; D009000:Monocytes / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D008050:Lipid A; D014409:Tumor Necrosis Factor-alpha
keywords: Bordetella; Bordetella petrii; Endotoxin; Lipid A; Structure–activity