title: Distribution of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in different stages of gestation sows: HP-PRRSV distribution in gestation sows.
abstract: Highly pathogenic PRRSV (HP-PRRSV) emerged in China in 2006 and caused severe reproductive losses, particularly in late-term sows. To determine whether these reproductive failures were related to the susceptibility of late-term sows to HP-PRRV, 60- and 90-days of gestation sows were infected with HP-PRRSV isolate TA-12 (GenBank accession HQ417620). A monoclonal antibody specific to the C-terminal of the nucleocapsid protein was used to evaluate viral distribution by IHC. This showed that HP-PRRSV had a similar distribution in both sets of sows. However, HP-PRRSV infection led to dramatically decreased serum levels of luteinizing hormone (LH) and 17-β-estradiol (E2) in late-term sows, while only E2 was decreased in the 60-day sows. These results indicate that HP-PRRSV-induced reproductive failure is more likely due to reproductive hormone level imbalances rather than tissue tropism differences.
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Hongkai
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Minxia
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Xinna
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Yajin
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Enmin
authors.initials: E
authors.lastname: Zhou
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: liusid@sdau.edu.cn.
authors.forename: Sidang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: xiaoyihong01@163.com.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D005865:Gestational Age; D007986:Luteinizing Hormone / Q000097:blood; D019318:Porcine Reproductive and Respiratory Syndrome / Q000503:physiopathology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D011374:Progesterone / Q000097:blood; D013552:Swine
chemical_list: D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Anti-N monoclonal antibody; Gestated sows; Highly pathogenic porcine reproductive and respiratory syndrome virus; Reproductive hormone; Virus distribution