title: Biotunable acoustic node assembly of organoids.
abstract: Bioengineering of 3D microtissues from cell spheroids is demonstrated by employing the vibration of acoustic standing waves and its hydrodynamic effect at the bottom of a liquid-carrier chamber. A large number of cell spheroids (>10(4) ) are assembled in seconds into a closely packed structure in a scaffold-free fashion under nodal pattern of the standing waves in a fluidic environment.
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center at Stanford for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Pu
authors.initials: P
authors.lastname: Chen
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center at Stanford for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Sinan
authors.initials: S
authors.lastname: Güven
authors.affiliation: Center for Engineering in Medicine at Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 02114, USA.
authors.forename: Osman Berk
authors.initials: OB
authors.lastname: Usta
authors.affiliation: Center for Engineering in Medicine at Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 02114, USA.|Department of Biomedical Engineering, Rutgers University, 599 Taylor Rd., Piscataway, NJ, 08854, USA.
authors.forename: Martin L
authors.initials: ML
authors.lastname: Yarmush
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center at Stanford for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
mesh_terms: D000162:Acoustics; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009940:Organoids / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D018874:Spheroids, Cellular / Q000166:cytology; D023822:Tissue Engineering
keywords: 3D co-culture; cell spheroids; scaffold-free assembly; spheroid fusion; tissue engineering