title: Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2 in Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL.
abstract: A novel powdery mildew resistance gene and a new allele of Pm1 were identified and fine mapped. DNA markers suitable for marker-assisted selection have been identified. Powdery mildew caused by Blumeria graminis is one of the most important foliar diseases of wheat and causes significant yield losses worldwide. Diploid A genome species are an important genetic resource for disease resistance genes. Two powdery mildew resistance genes, identified in Triticum boeoticum (A(b)A(b)) accession pau5088, PmTb7A.1 and PmTb7A.2 were mapped on chromosome 7AL. In the present study, shotgun sequence assembly data for chromosome 7AL were utilised for fine mapping of these Pm resistance genes. Forty SSR, 73 resistance gene analogue-based sequence-tagged sites (RGA-STS) and 36 single nucleotide polymorphism markers were designed for fine mapping of PmTb7A.1 and PmTb7A.2. Twenty-one RGA-STS, 8 SSR and 13 SNP markers were mapped to 7AL. RGA-STS markers Ta7AL-4556232 and 7AL-4426363 were linked to the PmTb7A.1 and PmTb7A.2, at a genetic distance of 0.6 and 6.0 cM, respectively. The present investigation established that PmTb7A.1 is a new powdery mildew resistance gene that confers resistance to a broad range of Bgt isolates, whereas PmTb7A.2 most probably is a new allele of Pm1 based on chromosomal location and screening with Bgt isolates showing differential reaction on lines with different Pm1 alleles. The markers identified to be linked to the two Pm resistance genes are robust and can be used for marker-assisted introgression of these genes to hexaploid wheat.
authors.affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Chhuneja
authors.affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
authors.forename: Bharat
authors.initials: B
authors.lastname: Yadav
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Stirnweis
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Severine
authors.initials: S
authors.lastname: Hurni
authors.affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
authors.forename: Satinder
authors.initials: S
authors.lastname: Kaur
authors.affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.|Wheat Research Department, Field Crops Research Institute, Agriculture Research Center, Giza, 12619, Egypt.
authors.forename: Ahmed Fawzy
authors.initials: AF
authors.lastname: Elkot
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Beat
authors.initials: B
authors.lastname: Keller
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Wicker
authors.affiliation: Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.|Department of Plant Science, South Dakota State University, Brookings, 57007, USA.
authors.forename: Sunish
authors.initials: S
authors.lastname: Sehgal
authors.affiliation: Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.
authors.forename: Bikram S
authors.initials: BS
authors.lastname: Gill
authors.affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India. kuldeep35@pau.edu.
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Singh
mesh_terms: D000483:Alleles; D001203:Ascomycota; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D004171:Diploidy; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D016324:Sequence Tagged Sites; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000382:microbiology
chemical_list: D005819:Genetic Markers