title: Multitarget super-resolution microscopy with high-density labeling by exchangeable probes.
abstract: We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.
authors.affiliation: Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.
authors.forename: Tai
authors.initials: T
authors.lastname: Kiuchi
authors.affiliation: Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.
authors.forename: Makio
authors.initials: M
authors.lastname: Higuchi
authors.affiliation: Laboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Sendai, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takamura
authors.affiliation: Laboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Sendai, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Maruoka
authors.affiliation: 1] Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan. [2] Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies, Kyoto, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D000199:Actins / Q000737:chemistry; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D020224:Expressed Sequence Tags; D005456:Fluorescent Dyes / Q000737:chemistry*; D022001:Focal Adhesions / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D008870:Microtubules / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D014982:Xenopus laevis
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D010455:Peptides; D049452:Green Fluorescent Proteins; D010100:Oxygen