title: Road blocks in making platelets for transfusion.
abstract: The production of laboratory-generated human platelets is necessary to meet present and future transfusion needs. This manuscript will identify and define the major roadblocks that must be overcome to make human platelet production possible for clinical use, and propose solutions necessary to accelerate development of laboratory-generated human platelets to market.
authors.affiliation: Hematology Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.|Harvard Medical School, Boston, MA, USA.|Platelet BioGenesis, Chestnut Hill, MA, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Thon
authors.affiliation: Hematology Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.|Harvard Medical School, Boston, MA, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Medvetz
authors.affiliation: Platelet BioGenesis, Chestnut Hill, MA, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Karlsson
authors.affiliation: Hematology Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.|Harvard Medical School, Boston, MA, USA.|Platelet BioGenesis, Chestnut Hill, MA, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Italiano
mesh_terms: D019149:Bioreactors; D001792:Blood Platelets / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D008533:Megakaryocytes / Q000502:physiology*; D010641:Phenotype; D017713:Platelet Transfusion; D055153:Stem Cell Niche; D034061:Thrombopoiesis / Q000235:genetics
keywords: biomedical engineering; bioreactors; hematopoietic stem cells; megakaryocytes; platelets