title: Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation.
abstract: The effects of the suppression of UDP-glucose pyrophosphorylase (UGPase) on chrysolaminaran biosynthesis and carbon allocation were investigated in Phaeodactylum tricornutum. The 69% decrease in UGPase activity was accompanied by a 4.89 fold reduction in Ugp transcript abundance. Inactivation of UGPase in P. tricornutum led to a significant decrease in chrysolaminaran content and an increase in lipid synthesis. These findings suggest that UGPase is a rate-limiting enzyme and may play an important role in chrysolaminarin biosynthesis and carbon allocation. Our results support a theoretical deduction that Ugp is a good candidate for improving lipid synthesis in diatoms.
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China. Electronic address: zhubaohua@ouc.edu.cn.
authors.forename: Bao-Hua
authors.initials: BH
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Shi
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Guan-Pin
authors.initials: GP
authors.lastname: Yang
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Lv
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China. Electronic address: qdkhpan@126.com.
authors.forename: Ke-Hou
authors.initials: KH
authors.lastname: Pan
mesh_terms: D001483:Base Sequence; D002244:Carbon / Q000378:metabolism*; D017377:Diatoms / Q000201:enzymology* / Q000254:growth & development; D020868:Gene Silencing; D005822:Genetic Vectors / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D002244:Carbon; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase