title: [The capacity of proliferation and migration is higher in Tca8113 cells with up-regulated TNFSF14 expression].
abstract: OBJECTIVE
  To construct a lentiviral vector carrying tumor necrosis factor superfamily member 14 (TNFSF14) gene, infect tongue cancer Tca8113 cells in vitro, and observe the effect on infected Tca8113 cells.
  
  
  METHODS
  A lentiviral vector containing TNFSF14 gene was constructed and used to infect the Tca8113 cells. After selected by puromycin, the level of TNFSF14 mRNA in Tca8113 cells was detected by real-time quantitative PCR. Cell proliferation activity and cell circle were determined respectively by MTT assay and flow cytometry (FCM). And the cell migration ability was measured by Transwell(TM) assay.
  
  
  RESULTS
  Compared with the control group, the expression of TNFSF14 mRNA increased in the infected cells. MTT assay and FCM showed TNFSF14 promoted the proliferation of Tca8113 cells. Transwell™ assay showed TNFSF14 boosted the migration ability of Tca8113 cells.
  
  
  CONCLUSIONS
  The proliferation and migration would be enhanced in Tca8113 cells with over-expressed TNFSF14.
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Weiyue
authors.initials: W
authors.lastname: Gao
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Jiakui
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Cheng
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Zishun
authors.initials: Z
authors.lastname: Qin
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Yin
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Zhanhai
authors.initials: Z
authors.lastname: Yu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D016193:G1 Phase / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D016196:S Phase / Q000235:genetics; D014062:Tongue Neoplasms / Q000473:pathology*; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D053326:Tumor Necrosis Factor Ligand Superfamily Member 14