title: Regulation of Stearoyl Coenzyme A Desaturase 1 Gene Promoter in Bovine Mammary Cells.
abstract: Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Bovine mammary epithelial cells (BME-UV1), cultured in vitro, have been proposed as a model to reproduce the biology of the mammary gland. The present study was designed to investigate the responsiveness of bovine SCD1 promoter to serum, insulin, oleic acid, and NFY transcription factor in BME-UV1 cells. A luciferase-based reporter assay was used to monitor the transcriptional activity of the SCD1 promoter region in BME-UV1 cells treated or not with insulin and/or oleic acid. The level of endogenous SCD1 mRNA was evaluated by Real time PCR. Insulin (20 ng/mL) induced a 2.0 to 2.5-fold increase of SCD1 promoter activity. Additionally, the effect of insulin was inhibited by oleic acid, serum components, and NFY enforced expression. Serum and NFY showed no synergistic or additive effect on SCD1 promoter activity suggesting that they repress SCD1 transcription through the same responsive element.
authors.affiliation: a Department of Biology , University of Naples "Federico II" , Naples , Italy.
authors.forename: O
authors.initials: O
authors.lastname: di Martino
authors.forename: A
authors.initials: A
authors.lastname: Troiano
authors.forename: L
authors.initials: L
authors.lastname: Addi
authors.forename: A
authors.initials: A
authors.lastname: Guarino
authors.forename: S
authors.initials: S
authors.lastname: Calabrò
authors.forename: R
authors.initials: R
authors.lastname: Tudisco
authors.forename: N
authors.initials: N
authors.lastname: Murru
authors.forename: M I
authors.initials: MI
authors.lastname: Cutrignelli
authors.forename: F
authors.initials: F
authors.lastname: Infascelli
authors.forename: V
authors.initials: V
authors.lastname: Calabrò
mesh_terms: D000818:Animals; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000201:enzymology*; D008969:Molecular Sequence Data; D019301:Oleic Acid / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D013230:Stearoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism
chemical_list: D023081:CCAAT-Binding Factor; D005227:Fatty Acids; D007328:Insulin; C402244:nuclear factor Y; D019301:Oleic Acid; D013230:Stearoyl-CoA Desaturase
keywords: Bovine mammary cells; Fatty acids; Insulin; Regulation of gene expression; Stearoyl coenzyme a desaturase