title: Improved differentiation of oligodendrocyte precursor cells and neurological function after spinal cord injury in rats by oscillating field stimulation.
abstract: Oscillating field stimulation (OFS) has been used in attempts to treat spinal cord injury (SCI) and has been shown to improve remyelination after SCI in rats. However, some controversies regarding the effects of OFS have been presented in previous papers. Oligodendrocytes (OLs) are the main cell for remyelination and are derived from the differentiation of oligodendrocyte precursor cells (OPCs). To date, it has been unclear whether the differentiation of OPCs can be regulated by OFS. The goal of this study was to determine if OFS can improve the differentiation of OPCs and promote the recovery of neurological function after SCI in rats. Immature and mature OLs were observed in spinal cord slices through immunofluorescence staining. Levels of adenosine triphosphate (ATP) and the cytokine leukemia inhibitory factor (LIF) were detected by enzyme-linked immunosorbent assay (ELISA). Basso-Beattie-Bresnahan (BBB) scores and transcranial magnetic motor-evoked potentials (tcMMEPs) were used to evaluate the locomotor outcomes of rats after SCI. Our results showed a significant improvement in the differentiation of OPCs and the content of ATP and LIF in the injured spinal cord in the OFS group. Furthermore, BBB scores and tcMMEPs were significantly improved in the rats stimulated by OFS. These findings suggest that OFS can improve the differentiation of OPCs and promote the recovery of neurological function following SCI in rats.
authors.affiliation: Department of Orthopaedics, The Second Hospital of AnHui Medical University, Furong Road 678, Hefei 230601, Anhui, China. Electronic address: jjhpaper@sina.com.
authors.forename: J-H
authors.initials: JH
authors.lastname: Jing
authors.affiliation: Department of Orthopaedics, The Second Hospital of AnHui Medical University, Furong Road 678, Hefei 230601, Anhui, China. Electronic address: qjpaper@sina.cn.
authors.forename: J
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Orthopaedics, The Second Hospital of AnHui Medical University, Furong Road 678, Hefei 230601, Anhui, China. Electronic address: zhunan1984@aliyun.com.cn.
authors.forename: N
authors.initials: N
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, The Second Hospital of AnHui Medical University, Furong Road 678, Hefei 230601, Anhui, China. Electronic address: tdspaper@sina.com.
authors.forename: W-B
authors.initials: WB
authors.lastname: Chou
authors.affiliation: Department of Orthopaedics, The Second Hospital of AnHui Medical University, Furong Road 678, Hefei 230601, Anhui, China. Electronic address: nilipaper@sohu.com.
authors.forename: X-J
authors.initials: XJ
authors.lastname: Huang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology / Q000503:physiopathology; D002454:Cell Differentiation / Q000502:physiology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D019054:Evoked Potentials, Motor / Q000502:physiology; D005260:Female; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D009043:Motor Activity / Q000502:physiology; D004676:Myelin Basic Protein / Q000378:metabolism; D009422:Nervous System Diseases / Q000209:etiology / Q000628:therapy*; D009836:Oligodendroglia / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000150:complications / Q000473:pathology* / Q000628:therapy; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors; D065908:Transcranial Direct Current Stimulation / Q000295:instrumentation
chemical_list: D053605:Leukemia Inhibitory Factor; D004676:Myelin Basic Protein; D000255:Adenosine Triphosphate
keywords: ATP; LIF; differentiation; oligodendrocyte progenitor cell; oscillating field stimulation; spinal cord injury