title: Antibacterial activity of herbal extracts against multi-drug resistant Escherichia coli recovered from retail chicken meat.
abstract: Increasing incidence rate of multiple drug resistance in Escherichia coli (E. coli) due to extensive uses of antibiotics is a serious challenge to disease treatment. Contaminated retail chicken meat is one of the major sources of spread of multi drug resistant (MDR) E. coli. Current study has been conducted to study the prevalence of MDR E. coli in retail chicken meat samples from Lahore city of Pakistan and it was found that 73.86% of E. coli isolates have MDR pattern. In vitro evaluation of antibacterial activity of crude ethanolic extracts of six herbs against MDR E. coli phenotypes has revealed that clove and cinnamon have maximum zones of inhibition as compared to other herbal extracts. Mint and coriander gave the intermediate results while garlic and kalonji showed the least antibacterial activity against the MDR E. coli phenotypes using the agar well diffusion technique. Average Minimum Inhibitory Concentrations (MICs) for clove, mint, cinnamon, coriander, kalonji and garlic extracts were 1.15, 1.38, 0.5, 1.99, 2.41, 8.60 mg/mL respectively using the broth micro dilution method. The results obtained in present study were revealed that crude ethanol extracts of selected herbs have had significant antibacterial activity. Hence they can be used as promising alternatives of antimicrobials against MDR E. coli species and can be used for cooked food preservation.
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Arfat Yousaf
authors.initials: AY
authors.lastname: Shaheen
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Ali Ahmad
authors.initials: AA
authors.lastname: Sheikh
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Masood
authors.initials: M
authors.lastname: Rabbani
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Asim
authors.initials: A
authors.lastname: Aslam
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Tasra
authors.initials: T
authors.lastname: Bibi
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Fakhra
authors.initials: F
authors.lastname: Liaqat
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Javed
authors.initials: J
authors.lastname: Muhammad
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Shafqat Fatima
authors.initials: SF
authors.lastname: Rehmani
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects*; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts