title: Alterations in Hippocampal Oxidative Stress, Expression of AMPA Receptor GluR2 Subunit and Associated Spatial Memory Loss by Bacopa monnieri Extract (CDRI-08) in Streptozotocin-Induced Diabetes Mellitus Type 2 Mice.
abstract: Bacopa monnieri extract has been implicated in the recovery of memory impairments due to various neurological disorders in animal models and humans. However, the precise molecular mechanism of the role of CDRI-08, a well characterized fraction of Bacopa monnieri extract, in recovery of the diabetes mellitus-induced memory impairments is not known. Here, we demonstrate that DM2 mice treated orally with lower dose of CDRI-08 (50- or 100 mg/kg BW) is able to significantly enhance spatial memory in STZ-DM2 mice and this is correlated with a significant decline in oxidative stress and up regulation of the AMPA receptor GluR2 subunit gene expression in the hippocampus. Treatment of DM2 mice with its higher dose (150 mg/kg BW or above) shows anti-diabetic effect in addition to its ability to recover the spatial memory impairment by reversing the DM2-induced elevated oxidative stress and decreased GluR2 subunit expression near to their values in normal and CDRI-08 treated control mice. Our results provide evidences towards molecular basis of the memory enhancing and anti diabetic role of the Bacopa monnieri extract in STZ-induced DM2 mice, which may have therapeutic implications.
authors.affiliation: Biochemistry & Molecular Biology Lab, Centre of Advanced Study in Zoology, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
authors.forename: Surya P
authors.initials: SP
authors.lastname: Pandey
authors.affiliation: Lumen Research Foundation, Ashok Nagar, Chennai, 600083, Tamilnadu, India.
authors.forename: Hemant K
authors.initials: HK
authors.lastname: Singh
authors.affiliation: Biochemistry & Molecular Biology Lab, Centre of Advanced Study in Zoology, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
authors.forename: S
authors.initials: S
authors.lastname: Prasad
mesh_terms: D000818:Animals; D034681:Bacopa / Q000737:chemistry; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism* / Q000523:psychology; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism* / Q000523:psychology; D004353:Drug Evaluation, Preclinical; D015870:Gene Expression / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018782:Maze Learning; D008569:Memory Disorders / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D065852:Spatial Memory / Q000187:drug effects; D013311:Streptozocin
chemical_list: C569478:BESEB CDRI-08; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D018091:Receptors, AMPA; D008315:Malondialdehyde; D013311:Streptozocin; C104722:glutamate receptor ionotropic, AMPA 2