title: A new method for evaluating the risk of transferring leukemic cells with transplanted cryopreserved ovarian tissue.
abstract: OBJECTIVE
  This study aimed to develop a method to detect ovarian residual disease by multicolor flow cytometry in acute leukemia patients.
  
  
  METHODS
  We designed an experimental model consisting in adding acute leukemia cells to a cell suspension obtained from healthy ovarian cortex. Leukemic cell detection within the ovarian cell suspension required the development of a specific myeloid antibody panel different from that commonly used for minimal residual disease (MRD) monitoring in bone marrow. The method was then used to detect ovarian residual disease in 11 acute leukemia patients.
  
  
  RESULTS
  Multicolor flow cytometry is able to evaluate the presence of viable leukemic cells in the ovarian cortex with good specificity and robust sensitivity of 10-4. We observed a good correlation between multicolor flow cytometry and quantitative polymerase chain reaction results. Ovarian residual disease detection by multicolor flow cytometry was positive in 3 out of 11 acute leukemia patients.
  
  
  CONCLUSIONS
  Multicolor flow cytometry can potentially be applied to ovarian tissue from all acute leukemia patients and is essential to evaluate the risk of cancer re-seeding before autograft of ovarian tissue in case of acute leukemia.
authors.affiliation: INSERM U 1098, CIC1431, 8, rue du Docteur Jean-François-Xavier GIROD, F-25020, Besançon Cedex, France, t1zver@chu-besancon.fr.
authors.forename: Tristan
authors.initials: T
authors.lastname: Zver
authors.forename: Magalie
authors.initials: M
authors.lastname: Alvergnas-Vieille
authors.forename: Francine
authors.initials: F
authors.lastname: Garnache-Ottou
authors.forename: Christophe
authors.initials: C
authors.lastname: Roux
authors.forename: Clotilde
authors.initials: C
authors.lastname: Amiot
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D018365:Neoplasm, Residual; D010051:Ovarian Neoplasms / Q000473:pathology* / Q000556:secondary*; D010053:Ovary / Q000637:transplantation*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D016044:Fusion Proteins, bcr-abl