title: Comprehensive Analysis of a Vibrio parahaemolyticus Strain Extracellular Serine Protease VpSP37.
abstract: Proteases play an important role in the field of tissue dissociation combined with regenerative medicine. During the years new sources of proteolytic enzymes have been studied including proteases from different marine organisms both eukaryotic and prokaryotic. Herein we have purified a secreted component of an isolate of Vibrio parahaemolyticus, with electrophoretic mobilities corresponding to 36 kDa, belonging to the serine proteases family. Sequencing of the N-terminus enabled the in silico identification of the whole primary structure consisting of 345 amino acid residues with a calculated molecular mass of 37.4 KDa. The purified enzyme, named VpSP37, contains a Serine protease domain between residues 35 and 276 and a canonical Trypsin/Chimotrypsin 3D structure. Functional assays were performed to evaluate protease activity of purified enzyme. Additionally the performance of VpSP37 was evaluated in tissue dissociations experiments and the use of such enzyme as a component of enzyme blend for tissue dissociation procedures is strongly recommended.
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola 91021, Trapani, Sicily, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Salamone
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola 91021, Trapani, Sicily, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Nicosia
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola 91021, Trapani, Sicily, Italy.
authors.forename: Carmelo
authors.initials: C
authors.lastname: Bennici
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF),Università di Palermo, Viale delle Scienze, edificio 16, Palermo, Sicily, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Quatrini
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF),Università di Palermo, Viale delle Scienze, edificio 16, Palermo, Sicily, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Catania
authors.affiliation: National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Calata porta di Massa, 80133, Napoli, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mazzola
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF),Università di Palermo, Viale delle Scienze, edificio 16, Palermo, Sicily, Italy; National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Calata porta di Massa, 80133, Napoli, Italy; ABIEL S.r.l., Via del Mare 3, Torretta Granitola 91021, Trapani, Sicily, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Ghersi
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola 91021, Trapani, Sicily, Italy; Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF),Università di Palermo, Viale delle Scienze, edificio 16, Palermo, Sicily, Italy; National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Calata porta di Massa, 80133, Napoli, Italy; ABIEL S.r.l., Via del Mare 3, Torretta Granitola 91021, Trapani, Sicily, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Cuttitta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004524:Eels / Q000382:microbiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D057057:Serine Proteases / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D057057:Serine Proteases