title: Microvessel permeability correlates with diabetic peripheral neuropathy in early stage of streptozotocin-induced diabetes rats.
abstract: OBJECTIVE
  This study aims to explore the alterations in microvessel permeability in the sciatic nerve and whether they are associated with the development of diabetic peripheral neuropathy (DPN) during the early stage of diabetes in rats.
  
  
  METHODS
  Sprague-Dawley rats were injected with streptozotocin and assessed at 0, 2, 4, and 8 weeks. Rats in the control group received the vehicle. Changes in sciatic nerve pathology, nerve conductive velocity (NCV), permeability of microvessel, and levels of vascular endothelial growth factor (VEGF) were examined.
  
  
  RESULTS
  The morphology and NCV of sciatic nerves showed signs of abnormality as early as 2 weeks after streptozotocin injection. The microvessel permeability as monitored by water and Evans blue content of sciatic nerve had increased dramatically at 4 and 8 weeks. The water and Evans blue content both negatively correlated with NCV. VEGF was found in axons as well as the myelin sheaths in diabetic rats but not in control rats.
  
  
  CONCLUSIONS
  The permeability of sciatic nerves was associated with the development of DPN in the early stage of diabetes in rats. Increased expression of VEGF may have a crucial role in changes in microvessel permeability and DPN.
authors.affiliation: Department of Endocrinology, Zunyi Medical College Affiliated Longgang Central Hospital, Shenzhen 518116, Guangdong, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Pathology, Zunyi Medical College Affiliated Longgang Central Hospital, Shenzhen 518116, Guangdong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, Zunyi Medical College Affiliated Longgang Central Hospital, Shenzhen 518116, Guangdong, China.
authors.forename: Fanglong
authors.initials: F
authors.lastname: Zhai
authors.affiliation: Department of Nursing, Zunyi Medical College Affiliated Longgang Central Hospital, Shenzhen 518116, Guangdong, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Endocrinology, Zunyi Medical College Affiliated Longgang Central Hospital, Shenzhen 518116, Guangdong, China. Electronic address: 13670298213@163.com.
authors.forename: Hailan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D002199:Capillary Permeability / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003929:Diabetic Neuropathies / Q000473:pathology*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D055806:Microvessels / Q000473:pathology* / Q000648:ultrastructure; D009431:Neural Conduction; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012307:Risk Factors; D012584:Sciatic Nerve / Q000098:blood supply* / Q000473:pathology; D012720:Severity of Illness Index; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Diabetes; Microvessel permeability; Peripheral neuropathy; VEGF; nerve conductive velocity