title: YvoA and CcpA Repress the Expression of chiB in Bacillus thuringiensis.
abstract: Bacillus thuringiensis produces chitinases, which are involved in its antifungal activity and facilitate its insecticidal activity. In our recent work, we found that a 16-bp sequence, drechiB (AGACTTCGTGATGTCT), downstream of the minimal promoter region of the chitinase B gene (chiB) was a critical site for the inducible expression of chiB in B. thuringiensis Bti75. In this work, we show that a GntR family transcriptional regulator (named YvoABt), which is homologous to YvoA of Bacillus subtilis, can specifically bind to the drechiB oligonucleotide sequences in vitro by using electrophoretic mobility shift assays (EMSAs) and isothermal titration calorimetry (ITC) assays. The results of quantitative real-time reverse transcription-PCR (qRT-PCR) and Western blotting indicated that deletion of yvoA caused an ∼7.5-fold increase in the expression level of chiB. Furthermore, binding of purified YvoABt to its target DNA could be abolished by glucosamine-6-phosphate (GlcN-6-P). We also confirmed, in the presence of the phosphoprotein Hpr-Ser₄₅-P, that purified CcpABt bound specifically to the promoter of chiB, which contains the "crechiB" sequence (ATAAAGCGTTTACA). According to the results of qRT-PCR and Western blotting, deletion of ccpA resulted in a 39-fold increase in the chiB expression level, and glucose no longer influenced the expression of chiB. We confirm that chiB is negatively controlled by both CcpABt and YvoABt in Bti75.
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Li
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Pan
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Wang
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China.
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China caijun@nankai.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
mesh_terms: D001413:Bacillus thuringiensis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D002688:Chitinases