title: Extracellular cyclophilin A possesses chemotaxic activity in cattle.
abstract: Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. satoru.takanashi.p2@dc.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. satoru.takanashi.p2@dc.tohoku.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Takanashi
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. nochi@m.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. nochi@m.tohoku.ac.jp.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Nochi
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. miku.abe.s2@dc.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. miku.abe.s2@dc.tohoku.ac.jp.
authors.forename: Miku
authors.initials: M
authors.lastname: Abe
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. vo1pe_d1_frago1a-02@docomo.ne.jp.
authors.forename: Nanami
authors.initials: N
authors.lastname: Itaya
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. urakawa@bios.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. urakawa@bios.tohoku.ac.jp.
authors.forename: Megumi
authors.initials: M
authors.lastname: Urakawa
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. katsuyoshi-sato@akita-pu.ac.jp.
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Sato
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. zhuang.tao.r3@dc.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. zhuang.tao.r3@dc.tohoku.ac.jp.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhuang
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. gmag.529@gmail.com.
authors.forename: Saori
authors.initials: S
authors.lastname: Umemura
authors.affiliation: National Agriculture and Food Research Organization, National Institute of Animal Health, Hokkaido, 062-0045, Japan. hayatomo@affrc.go.jp.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: National Agriculture and Food Research Organization, National Institute of Animal Health, Hokkaido, 062-0045, Japan. yokiku@affrc.go.jp.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiku
authors.affiliation: International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. haruki@bios.tohoku.ac.jp.|Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. haruki@bios.tohoku.ac.jp.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, SY23 3DA, UK. mir@aber.ac.uk.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Rose
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. watakoh@bios.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. watakoh@bios.tohoku.ac.jp.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Laboratory of Mucosal Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. asosan@bios.tohoku.ac.jp.|International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 981-8555, Japan. asosan@bios.tohoku.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
mesh_terms: D000818:Animals; D002417:Cattle; D002633:Chemotaxis; D021984:Cyclophilin A / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000276:immunology / Q000502:physiology*; D008414:Mastitis, Bovine / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D021984:Cyclophilin A