title: miR-503 suppresses metastasis of hepatocellular carcinoma cell by targeting PRMT1.
abstract: Accumulating evidence indicates that microRNAs function as oncogenes or tumor suppressor genes in human cancer. MiR-503 is deregulated in various human cancers and has been associated with hepatocellular carcinoma (HCC) progression. However, the underlying mechanisms of miR-503 involvement in the development and progression of HCC remains poorly understood. In the present study, we report that miR-503 suppresses cell metastasis in HCC through targeting the protein arginine methyl transferase 1 (PRMT1) mRNA. Notably, we identiﬁed that miR-503 was able to target 3'-untranslated region (3'-UTR) of PRMT1 mRNA by luciferase reporter gene assays. Then, we revealed that miR-503 was able to reduce the expression of PRMT1 at the levels of mRNA and protein using RT-PCR and Western blotting analysis. The expression levels of miR-503 were negatively related to those of PRMT1 mRNA in clinical HCC tissues. In terms of function, transwell and wound healing assays demonstrated that the miR-503 remarkably inhibited invasion and migration of HCC cells, which was reversed by overexpressed PRMT1. Furthermore, exogenous expression of miR-503 dramatically suppressed epithelial-mesenchymal transition (EMT) via PRMT1 in HCC cells. In conclusion, we denomstrated PRMT1 as a novel target gene of miR-503 and miR-503-mediated PRMT1 could also emerge as a potential important biomarker for HCC.
authors.affiliation: Department of Surgery, Shandong Provincial Hospital, 4 Duanxingxi Road, Jinan 250022, PR China. Electronic address: bingshouli2324@163.com.
authors.forename: Bingshou
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Surgery, Shandong Provincial Hospital, 4 Duanxingxi Road, Jinan 250022, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Surgery, Shandong Provincial Hospital, 4 Duanxingxi Road, Jinan 250022, PR China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Surgery, Shandong Provincial Hospital, 4 Duanxingxi Road, Jinan 250022, PR China.
authors.forename: Lingtong
authors.initials: L
authors.lastname: Wu
mesh_terms: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism* / Q000556:secondary; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000235:genetics / Q000517:prevention & control; D011484:Protein-Arginine N-Methyltransferases / Q000037:antagonists & inhibitors* / Q000235:genetics*; D012097:Repressor Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; C545473:MIRN503 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases
keywords: EMT; HCC; PRMT1; miR-503