title: Optimization of high solids fed-batch saccharification of sugarcane bagasse based on system viscosity changes.
abstract: Viscosity trends in alkali-pretreated sugarcane bagasse (SCB) slurries undergoing high solids fed-batch enzymatic hydrolysis were measured for a range of solids loading from 15% to 36%. Solids liquefaction times were related to system viscosity changes. The viscosity decreased quickly for low solids loading, and increased with increasing solids content. Fed-batch hydrolysis was initiated with 15% solids loading, and an additional 8%, 7% and 6% were successively added after the system viscosity decreased to stable values to achieve a final solids content of 36%. Two enzyme-adding modes with 8.5FPU/g solid were investigated. The batch mode with all enzyme being added at the beginning of the reaction produced the highest yields, with approximately 231.7g/L total sugars and 134.9g/L glucose being obtained after 96h with nearly 60% of the final glucan conversion rate. This finding indicates that under the right conditions, the fed-batch strategy might be a plausible way to produce high sugars under high solids.
authors.affiliation: Institute of New Energy and New Materials, South China Agriculture University, Guangzhou 510642, PR China; Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China. Electronic address: yuanzh@ms.giec.ac.cn.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Institute of New Energy and New Materials, South China Agriculture University, Guangzhou 510642, PR China. Electronic address: xiejun@scau.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
mesh_terms: D000468:Alkalies / Q000494:pharmacology; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002482:Cellulose / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D031786:Saccharum / Q000737:chemistry* / Q000187:drug effects; D013997:Time Factors; D014783:Viscosity; D014994:Xylose / Q000032:analysis
chemical_list: D000468:Alkalies; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D005947:Glucose
keywords: Alkali-pretreated SCB; Enzymatic saccharification; Fed-batch strategy; High concentration sugars; Viscosity