title: The influence of biofilms on the mobility of bare and capped zinc oxide nanoparticles in saturated sand and glass beads.
abstract: Biofilms are a common constituent of the subsurface and are known to influence contaminant transport; however only a few studies to date have addressed microbial controls on nanoparticle mobility in porous media. The impact of a 3-day Pantoea agglomerans biofilm on the mobility of zinc oxide (ZnO) nanoparticles was studied in column experiments containing sand and glass beads at near-neutral pH and constant ionic strength. Bare ZnO nanoparticles (bZnO-NPs) and ZnO nanoparticles capped with tri-aminopropyltriethoxysilane (cZnO-NPs) were used in the experiments. Breakthrough curves demonstrate that the biofilm particularly slowed nanoparticle migration of bZnO-NPs in glass bead columns and cZnO-NPs in sand columns. With the exception of bZnO-NPs in sand columns, biofilm-coated porous media retained more nanoparticles than those of controls without biofilm. The biofilm may bear an impact on the surface charge of the porous medium, nullifying porous medium-specific effects. Although viable cell counts (VCCs) decreased after the introduction of electrolyte and before nanoparticle transport experiments, SEM and CLSM imaging of porous medium samples taken from columns after nanoparticle transport experiments, as well as total organic carbon (TOC) measurements reveal that biofilm was present in the columns throughout the experiments. Hence, it can be concluded that even a thin amount of biofilm can hinder nanoparticle migration in small-scale porous medium experiments. Moreover, nanoparticle mobility is dependent on the binding capacity of biofilms, rather than the type of porous media.
authors.affiliation: Mott MacDonald Polska Sp. z o.o., ul. Waliców 11, 00-851 Warsaw, Poland. Electronic address: hanna.kurlanda@yahoo.com.
authors.forename: H
authors.initials: H
authors.lastname: Kurlanda-Witek
authors.affiliation: School of GeoSciences, University of Edinburgh Kings Buildings, West Mains Rd, EH9 3JW Edinburgh, United Kingdom.
authors.forename: B T
authors.initials: BT
authors.lastname: Ngwenya
authors.affiliation: School of GeoSciences, University of Edinburgh Kings Buildings, West Mains Rd, EH9 3JW Edinburgh, United Kingdom.
authors.forename: I B
authors.initials: IB
authors.lastname: Butler
mesh_terms: D018441:Biofilms; D002244:Carbon / Q000032:analysis; D005898:Glass; D006863:Hydrogen-Ion Concentration; D062070:Hydrology / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D020636:Pantoea / Q000502:physiology*; D016062:Porosity; D012822:Silicon Dioxide; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis; D015034:Zinc Oxide
chemical_list: D014874:Water Pollutants, Chemical; D002244:Carbon; D012822:Silicon Dioxide; D015034:Zinc Oxide
keywords: Biofilms; Nanoparticles; Porous media