title: Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic medium.
abstract: A commercially available convective interaction media (CIM) Protein A monolithic column was fully characterized in view of its application for the affinity capture of IgG in monoclonal antibody production processes. By means of moment analysis, the interstitial porosity and axial dispersion coefficient were determined. The frontal analysis method of characteristic points was employed, for the first time with monolithic media, to determine the dynamic binding capacity. The effects of the flow rate and pH on the total recovery of polyclonal IgG and elution profile were evaluated. A comparison with literature data for Protein A chromatography beads demonstrate the superior bed utilization of monolithic media, which gave better performance at lower residence times.
authors.affiliation: Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Università di Bologna, via Terracini 28, 40132 Bologna, Italy.
authors.forename: M Omon
authors.initials: MO
authors.lastname: Herigstad
authors.affiliation: Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Università di Bologna, via Terracini 28, 40132 Bologna, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Dimartino
authors.affiliation: Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Università di Bologna, via Terracini 28, 40132 Bologna, Italy. Electronic address: cristiana.boi@unibo.it.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Boi
authors.affiliation: Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Università di Bologna, via Terracini 28, 40132 Bologna, Italy.
authors.forename: Giulio C
authors.initials: GC
authors.lastname: Sarti
mesh_terms: D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification; D002845:Chromatography / Q000592:standards*; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification*; D016062:Porosity; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A
keywords: Affinity; Elution; IgG; Monoliths; Protein A; Transport properties