title: Antioxidant Activity and Induction of mRNA Expressions of Antioxidant Enzymes in HEK-293 Cells of Moringa oleifera Leaf Extract.
abstract: The leaves of Moringa oleifera, collected in different provinces in Thailand, were determined for the contents of total phenolics, total flavonoids, major components, and antioxidant activity. The extract and its major active components were investigated for the inhibition of H2O2-induced reactive oxygen species production and the effects on antioxidant enzymes mRNA expression. The extract, crypto-chlorogenic acid, isoquercetin and astragalin, significantly reduced the reactive oxygen species production inducing by H2O2 in HEK-293 cells. Treatment with isoquercetin significantly increased the mRNA expression levels of antioxidant enzymes such as superoxide dismutase, catalase and heme oxygenase 1. These results confirm that M. oleifera leaves are good sources of natural antioxidant with isoquercetin as an active compound.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Boonyadist
authors.initials: B
authors.lastname: Vongsak
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Supachoke
authors.initials: S
authors.lastname: Mangmool
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Wandee
authors.initials: W
authors.lastname: Gritsanapan
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002726:Chlorogenic Acid / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D057809:HEK293 Cells; D051547:Heme Oxygenase-1 / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D044949:Kaempferols / Q000378:metabolism; D029588:Moringa oleifera / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D044949:Kaempferols; D010636:Phenols; D010936:Plant Extracts; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; C001579:astragalin; D006861:Hydrogen Peroxide; D002374:Catalase; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase