title: Intramyocardial delivery of VEGF165 via a novel biodegradable hydrogel induces angiogenesis and improves cardiac function after rat myocardial infarction.
abstract: Vascular endothelial growth factor (VEGF), an independent mitogen, has been reported to induce angiogenesis and thus attenuates the damage induced by myocardial infarction (MI). VEGF165 is the most abundant and predominant isoform of VEGF. This study investigates whether this effect could be strengthened by local intramyocardial injection of VEGF165 along with a novel biodegradable Dex-PCL-HEMA/PNIPAAm hydrogel and ascertains its possible mechanism of action. Rat models of myocardial infarction were induced by coronary artery ligation. Phosphate-buffered saline (PBS group), Dex-PCL-HEMA/PNIPAAm hydrogel (Gel group), phosphate-buffered saline containing VEGF165 (VP group), and hydrogel containing VEGF165 (VPG group) were injected into a peri-infarcted area of cardiac tissue immediately after myocardial infarction, respectively. The sham group was thoracic but without myocardial infarction. The injection of VEGF165 along with a hydrogel induced angiogenesis, reduced collagen content and MI area, inhibited cell apoptosis, increased the level of VEGF165 protein and the expression of flk-1 and flt-1, and improved cardiac function compared with the injection of either alone after MI in rats. The results suggest that injection of VEGF165 along with a hydrogel acquires more cardioprotective effects than either alone in rat with MI by sustained release of VEGF165, then may enhance the feedback between VEGF and its receptors flk-1 and flt-1.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. xjjiang@whu.edu.cn.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China. xjjiang@whu.edu.cn.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Miaoyang
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Wan
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Yiyu
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, People's Republic of China.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry; D004195:Disease Models, Animal; D004337:Drug Carriers; D004339:Drug Compounding; D020100:Hydrogels; D015552:Injections, Intralesional; D007700:Kinetics; D008297:Male; D008689:Methacrylates / Q000737:chemistry; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D015398:Signal Transduction / Q000187:drug effects; D012995:Solubility; D013318:Stroke Volume / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage* / Q000737:chemistry; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D016277:Ventricular Function, Left / Q000187:drug effects*
chemical_list: D000180:Acrylic Resins; D043925:Angiogenesis Inducing Agents; D003911:Dextrans; D004337:Drug Carriers; D020100:Hydrogels; D008689:Methacrylates; D011091:Polyesters; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; C016240:polycaprolactone; C052970:poly-N-isopropylacrylamide; C005044:hydroxyethyl methacrylate; D003094:Collagen; C501164:Flt1 protein, rat; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: Angiogenesis; Hydrogel; Myocardial infarction; VEGF165