title: Rana catesbeianaribonuclease induces cell apoptosis via the caspase-9/-3 signaling pathway in human glioblastoma DBTRG, GBM8901 and GBM8401 cell lines.
abstract: Human glioblastoma multiforme is one of the most aggressive malignant brain tumor types, and the mean survival time of patients with a brain tumor is <2 years when traditional therapies are administered. Thus, numerous studies have focused on the development of novel treatments for brain tumors. Frog ribonucleases, such as Onconase andRana catesbeianaribonuclease (RC-RNase), exert antitumor effects on various tumor cells, including cervical cancer, breast cancer, hepatoma, leukemia, pancreatic cancer and prostate cancer cells. In addition, frog Onconase has been applied as a treatment in clinical trials. However, the antitumor effects of frog ribonucleases on brain tumors are unclear. Previous studies have indicated that RC-RNase demonstrates a decreased cytotoxic effect in normal cells compared with Onconase. Therefore, the present study investigated the ability of RC-RNase to exert antitumor activities on human glioblastoma. It was found that RC-RNase inhibits the growth of the human glioblastoma DBTRG, GBM8901 and GBM8401 cells. In addition, the present study revealed that RC-RNase induces caspase-9/-3 activity and triggers the apoptotic cell death pathway in human glioblastoma cells. Notably, it was also demonstrated that RC-RNase effectively inhibits the growth of human glioblastoma tumors in a nude mouse model. Overall, the present study indicates that RC-RNase may be a potential agent for the treatment of human glioblastoma.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, R.O.C. ; Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, Taichung 433, Taiwan, R.O.C.
authors.forename: Jen-Ni
authors.initials: JN
authors.lastname: Chen
authors.affiliation: Department of Emergency Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei 231, Taiwan, R.O.C. ; Department of Emergency Medicine, School of Medicine, Tzu Chi University, Hualien 970, Taiwan, R.O.C.
authors.forename: Giou-Teng
authors.initials: GT
authors.lastname: Yiang
authors.affiliation: Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan, R.O.C.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Lin
authors.affiliation: Division of Allergy-Immunology-Rheumatology, Department of Internal Medicine, Saint Mary's Hospital Luodong, Luodong, Yilan 265, Taiwan, R.O.C. ; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan, R.O.C.
authors.forename: Pei-Lun
authors.initials: PL
authors.lastname: Chou
authors.affiliation: The PhD Program for Cancer Biology and Drug Discovery, China Medical University, Taichung 404, Taiwan, R.O.C. ; Division of Renal Medicine, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan, R.O.C.
authors.forename: Tsai-Kun
authors.initials: TK
authors.lastname: Wu
authors.affiliation: Graduate Institute of Cancer Biology, Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan, R.O.C.
authors.forename: Wei-Jung
authors.initials: WJ
authors.lastname: Chang
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, R.O.C.
authors.forename: Chinshuh
authors.initials: C
authors.lastname: Chen
authors.affiliation: The PhD Program for Cancer Biology and Drug Discovery, China Medical University, Taichung 404, Taiwan, R.O.C. ; Graduate Institute of Cancer Biology, Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan, R.O.C. ; Department of Biotechnology, Asia University, Taichung 413, Taiwan, R.O.C.
authors.forename: Yung-Luen
authors.initials: YL
authors.lastname: Yu
keywords: apoptosis; brain tumor; caspase; frog ribonulease