title: Simple determination of fluoride in biological samples by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry.
abstract: A simple and convenient method to detect fluoride in biological samples was developed. This method was based on derivatization with 2-(bromomethyl)naphthalene, headspace solid phase microextraction (HS-SPME) in a vial, and gas chromatography-tandem mass spectrometric detection. The HS-SPME parameters were optimized as follows: selection of CAR/PDMS fiber, 0.5% 2-(bromomethyl)naphthalene, 250 mg/L 15-crown-5-ether as a phase transfer catalyst, extraction and derivatization temperature of 95 °C, heating time of 20 min and pH of 7.0. Under the established conditions, the lowest limits of detection were 9 and 11 μg/L in 1.0 ml of plasma and urine, respectively, and the intra- and inter-day relative standard deviation was less than 7.7% at concentrations of 0.1 and 1.0 mg/L. The calibration curve showed good linearity of plasma and urine with r=0.9990 and r=0.9992, respectively. This method is simple, amenable to automation and environmentally friendly.
authors.affiliation: Department of Environmental Science, Kongju National University, Kongju, 314-701, South Korea.
authors.forename: Sun-Myung
authors.initials: SM
authors.lastname: Kwon
authors.affiliation: Department of Environmental Education, Kongju National University, Kongju 314-701, South Korea. Electronic address: hshin@kongju.ac.kr.
authors.forename: Ho-Sang
authors.initials: HS
authors.lastname: Shin
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D005459:Fluorides / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D052617:Solid Phase Microextraction; D013696:Temperature; D016482:Urinalysis / Q000379:methods*
chemical_list: D005459:Fluorides
keywords: Biological samples; Fluoride; Gas chromatography–tandem mass spectrometry; Headspace solid phase microextraction