title: Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.
abstract: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Montesi
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Panseri
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Iafisco
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Adamiano
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
mesh_terms: D000818:Animals; D015519:Bone Density; D002454:Cell Differentiation; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D007781:Lactoferrin / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D017886:Durapatite; D007781:Lactoferrin