title: MA104 Cell line presents characteristics suitable for enterovirus A71 isolation and proliferation.
abstract: Enterovirus A71 (EV-A71), one of the most important causative agents of hand, foot and mouth disease (HFMD) in children, can lead to severe clinical outcomes, even death. However, the infection spectrum of EV-A71 in different cell lines remains unknown. Therefore, in this study, the biological characteristics of EV-A71 Subgroup C4 in different cell lines were investigated. To this end, the infectivity of EV-A71Jinan1002 isolated from children with severe HFMD was assessed in 18 different host cell lines. It was found that the MA104 cell line displayed biological characteristics suitable for EV-A71 Subgroup C4 strain isolation and proliferation; indeed, it was found that a broad spectrum of cell lines can be infected by EV-A71Jinan1002. Among the screened cells, four cell lines (HEK293, RD, MA104 and Marc145) produced high 50% tissue culture infective dose (TCID50 ) values calculated in viral proliferations (ranged from 10(7.6) to 10(7.8) ); the TCID50 being negatively associated with the time to appearance of CPE. Proliferation curves demonstrated that EV-A71Jinan1002 amplifies more efficiently in MA104, Hep-2 and RD cells. Remarkably, the virus isolation rate was much higher in MA104 cells than in RD cells. Thus this study, to our knowledge, is for the first to explore the infection spectrum of EV-A71 subgroup C4 in such a large number of different cell lines. Our data provide useful reference data for facilitating further study of EV-A71.
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Yue
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Song
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Li
authors.affiliation: Jinan Infectious Disease Hospital, 22029 Jingshi Road, Jinan 250021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Hong
authors.initials: H
authors.lastname: Meng
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Jiang
authors.affiliation: Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Rare and Uncommon Diseases, 18877 Jingshi Road, Jinan 250062.
authors.forename: Lizeng
authors.initials: L
authors.lastname: Qin
mesh_terms: D000818:Animals; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D003588:Cytopathogenic Effect, Viral; D029821:Enterovirus A, Human / Q000254:growth & development* / Q000302:isolation & purification*; D006232:Hand, Foot and Mouth Disease / Q000821:virology; D006801:Humans; D014776:Virus Cultivation / Q000379:methods*
keywords: biological characteristics; enterovirus A71 (EV-A71); host cells