title: Rapid detection of urinary long non-coding RNA urothelial carcinoma associated one using a PCR-free nanoparticle-based assay.
abstract: We developed a specific hybridization assay for direct detection of long non-coding RNA urothelial carcinoma associated-1 (lncRNA-UCA1). Total RNA was extracted from urine pellet samples (bladder carcinoma patients and controls). Then, we compared the developed nanoassay with quantitative real time polymerase chain reaction (qRT-PCR) results in detection of urine UCA1 in bladder cancer and control samples. The sensitivity and the specificity of UCA1 nanoassay were 92.1% and 93.3%, respectively. The concordance of the two methods was 98%. Interestingly, all bilharzial benign cases showed negative lncRNA-UCA1 using both methods. UCA1-nanoassay is a valid test for direct detection of urine UCA1 for bladder cancer detection.
authors.affiliation: a Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt .
authors.forename: Sanaa
authors.initials: S
authors.lastname: Eissa
authors.forename: Marwa
authors.initials: M
authors.lastname: Matboli
authors.forename: Nada O E
authors.initials: NO
authors.lastname: Essawy
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Shehta
authors.forename: Youssef M
authors.initials: YM
authors.lastname: Kotb
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001681:Biological Assay; D014408:Biomarkers, Tumor / Q000235:genetics / Q000652:urine*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D053758:Nanoparticles / Q000737:chemistry; D062085:RNA, Long Noncoding / Q000235:genetics / Q000652:urine*; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C515159:UCA1 RNA, human
keywords: Bilharziasis; bladder cancer; gold nanoparticles; long non-coding RNA-UCA1; urine biomarkers; urine cytology