title: Lentivirus-mediated RNA interference targeting UbcH10 reduces cell growth and invasion of human osteosarcoma cells via inhibition of Ki-67 and matrix metalloproteinases.
abstract: Osteosarcoma (OS) is the most commonly diagnosed primary malignancy affecting the bone. UbcH10 is a cancer-related E2-ubiquitin-conjugating enzyme. An overexpression of UbcH10 is significantly associated with tumor grade and cellular proliferation. However, limited evidence exists with regard to the biological function of UbcH10 in OS. The present study created a UbcH10 knockdown OS cell line using lentivirus-mediated RNA interference. The expression of UbcH10 was significantly reduced in UbcH10-targeted small hairpin RNA-expressing lentivirus OS cells. The downregulation of UbcH10 suppressed OS cell proliferation and colony formation ability via decreased Ki-67 expression. UbcH10 knockdown OS cells exhibited impaired invasion and migration abilities. Furthermore, knockdown of UbcH10 led to decreased levels of matrix metalloproteinase-3 and -9 in OS cells. The present study demonstrated the role of UbcH10 in OS cell proliferation, invasion and migration, which suggests that UbcH10 may be a potential candidate for OS therapy.
authors.affiliation: Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong 510012, P.R. China ; Department of Orthopedics, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China.
authors.forename: Shan-Tao
authors.initials: ST
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China.
authors.forename: Dan-Zhi
authors.initials: DZ
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong 510012, P.R. China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Orthopedics, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China.
authors.forename: Hua-Zhuang
authors.initials: HZ
authors.lastname: Li
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310006, P.R. China.
authors.forename: Pei-Jian
authors.initials: PJ
authors.lastname: Tong
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310006, P.R. China.
authors.forename: Fu-Cun
authors.initials: FC
authors.lastname: Liu
keywords: Ki-67; UbcH10; growth; invasion; matrix metalloproteinases; osteosarcoma