title: SLC6A14 and 5-HTR2C polymorphisms are associated with food intake and nutritional status in children.
abstract: BACKGROUND
  Serotonin plays a critical role in the regulation of food intake. The solute carrier family 6 member 14 (SLC6A14) and serotonin receptor 2C (5-HTR2C) genes are involved in the bioavailability and action of this neurotransmitter.
  
  
  OBJECTIVE
  Evaluation of the association of six polymorphisms in these genes with food intake and nutritional status in children followed to 7-8years of age.
  
  
  METHODS
  Blood samples and the biodemographic data of 344 children were collected at three development stages, in a cross-sectional study undertaken with the cohort from a randomized trial. Polymorphisms were analyzed using polymerase chain reaction-based techniques.
  
  
  RESULTS
  At 7 to 8years of age, carriers of the A alleles for both the SLC6A14 rs2312054 and SLC6A14 rs12391221 polymorphisms showed higher food intake, except for the sugar-dense food (SDF) intake parameter, than T/T and C/C homozygotes, respectively. Boy carriers of the C allele of rs2071877 had a higher sum of triceps and subscapular folds than T allele carriers at 7 to 8years old. For 5-HTR2C gene variants, A allele carriers (rs3813928) and T allele carriers (rs3813929) had higher food intake at 3 to 4years old than G/G and C/C homozygotes, respectively, except for SDF. At this age, the intake of energy-dense foods was higher in carriers of the T allele (rs3813929) than in C/C homozygotes.
  
  
  CONCLUSIONS
  This study provides evidence that genetic variants of these proteins might be involved in the determination of food intake and nutritional status in children.
authors.affiliation: Laboratório de Biologia Molecular, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245/Anexo 2 - sala 07, CEP 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Raquel C K
authors.initials: RC
authors.lastname: Miranda
authors.affiliation: Laboratório de Biologia Molecular, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245/Anexo 2 - sala 07, CEP 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Vetter
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245 - sala 309, CEP 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Júlia P
authors.initials: JP
authors.lastname: Genro
authors.affiliation: Núcleo de Pesquisa em Nutrição (NUPEN), Departamento de Nutrição, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245/Anexo 2 - sala 03, CEP 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Paula D B
authors.initials: PD
authors.lastname: Campagnolo
authors.affiliation: Laboratório de Biologia Molecular, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245/Anexo 2 - sala 07, CEP 90050-170, Porto Alegre, RS, Brazil; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245 - sala 309, CEP 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Mattevi
authors.affiliation: Núcleo de Pesquisa em Nutrição (NUPEN), Departamento de Nutrição, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245/Anexo 2 - sala 03, CEP 90050-170, Porto Alegre, RS, Brazil.
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Vitolo
authors.affiliation: Laboratório de Biologia Molecular, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245/Anexo 2 - sala 07, CEP 90050-170, Porto Alegre, RS, Brazil; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245 - sala 309, CEP 90050-170, Porto Alegre, RS, Brazil. Electronic address: silvana.almeida@pq.cnpq.br.
authors.forename: Silvana
authors.initials: S
authors.lastname: Almeida
mesh_terms: D000483:Alleles; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004435:Eating / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007223:Infant; D008297:Male; D009752:Nutritional Status / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D016032:Randomized Controlled Trials as Topic; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics*
chemical_list: D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D044405:Receptor, Serotonin, 5-HT2C; C503995:SLC6A14 protein, human
keywords: Children; Eating; Genetic polymorphism; Obesity; Serotonin