title: Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil.
abstract: An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed.
authors.affiliation: Laboratório de Parasitologia e Epidemiologia Molecular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Cláudio
authors.initials: C
authors.lastname: Mafra
authors.affiliation: Laboratório de Parasitologia e Epidemiologia Molecular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Mantovani
authors.affiliation: Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Juliana Novo
authors.initials: JN
authors.lastname: Borges
authors.affiliation: Laboratório de Parasitologia e Epidemiologia Molecular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Rafael Mazioli
authors.initials: RM
authors.lastname: Barcelos
authors.affiliation: Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Cláudia Portes
authors.initials: CP
authors.lastname: Santos
mesh_terms: D000818:Animals; D017191:Ascaridida Infections / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000648:ultrastructure; D001938:Brazil; D005393:Fish Diseases / Q000175:diagnosis* / Q000469:parasitology*; D019649:Food Industry; D048388:Gadiformes / Q000469:parasitology*; D007814:Larva; D008855:Microscopy, Electron, Scanning; D025202:Molecular Diagnostic Techniques