title: Expression pattern of immediate early genes in the cerebellum of D1R KO, D2R KO, and wild type mice under vestibular-controlled activity.
abstract: We previously reported the different motor abilities of D1R knockout (KO), D2R KO and wild-type (WT) mice. To understand the interaction between the cerebellum and the striatal direct and indirect pathways, we examined the expression patterns of immediate early genes (IEG) in the cerebellum of these three genotypes of mice. In the WT naive mice, there was little IEG expression. However, we observed a robust expression of c-fos mRNA in the vermis and hemisphere after running rota-rod tasks. In the vermis, c-fos was expressed throughout the lobules except lobule 7, and also in crus 1 of the ansiform lobule (Crus1), copula of the pyramis (Cop) and most significantly in the flocculus in the hemisphere. jun-B was much less expressed but more preferentially expressed in Purkinje cells. In addition, we observed significant levels of c-fos and jun-B expressions after handling mice, and after the stationary rota-rod task in naive mice. Surprisingly, we observed significant expression of c-fos and jun-B even 30 min after single weighing. Nonetheless, certain additional c-fos and jun-B expressions were observed in three genotypes of the mice that experienced several sessions of motor tasks 24 h after stationary rota-rod task and on days 1 and 5 after rota-rod tasks, but no significant differences in expressions after the running rota-rod tasks were observed among the three genotypes. In addition, there may be some differences 24 h after the stationary rota-rod task between the naive mice and the mice that experienced several sessions of motor tasks.
authors.affiliation: Division of Brain Biology, National Institute for Basic Biology Okazaki, Japan ; Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI) Okazaki, Japan ; KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University Suita, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Laboratory of Neurochemistry, National Institute for Basic Biology Okazaki, Japan ; Department of Laboratory Animal Science, Kitasato University School of Medicine Sagamihara, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Sato
authors.affiliation: Division of Brain Biology, National Institute for Basic Biology Okazaki, Japan ; KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University Suita, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kitsukawa
authors.affiliation: Laboratory of Neurochemistry, National Institute for Basic Biology Okazaki, Japan ; Department of Laboratory Animal Science, Kitasato University School of Medicine Sagamihara, Japan ; Department of Comparative and Experimental Medicine, Brain Research Institute, Niigata University Niigata, Japan.
authors.forename: Toshikuni
authors.initials: T
authors.lastname: Sasaoka
authors.affiliation: Division of Brain Biology, National Institute for Basic Biology Okazaki, Japan ; Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI) Okazaki, Japan ; Laboratory of Molecular Analysis for Higher Brain Function, RIKEN Brain Science Institute Wako, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamamori
keywords: IEG; c-fos; jun-B; rota-rod; striatum