title: Impact on red blood cell immunity patterns in postoperative phase following total hip arthroplasty.
abstract: OBJECTIVE
  In this study, we aimed to measure changes in red blood cell (RBC) immunity and cytokine levels after performing total hip replacement surgery.
  
  
  METHODS
  Twenty patients receiving total hip arthroplasty were investigated by measuring presurgical and postoperative RBC natural tumor erythrocyte rosette rate (NTERR), RBC C3b receptor rosette rate (RC3bRR), RBC membrane CD35, CD58 and CD59 expression and cytokine levels [including tumor necrosis factor α (TNF-α), interleukin 2 (IL-2), interferon γ (IFN-γ), interleukin 10 (IL-10) and prostaglandin E2 (PGE2)]. Blood samples were collected on the day before surgery and on the first day after hip arthroplasty.
  
  
  RESULTS
  Postoperative NTERR and RC3bRR were significantly lower than presurgical levels (p < 0.05). The RBC membrane CD35, CD58 and CD59 expressions were significantly decreased in the postoperative phase compared to pre-operative levels. Importantly, RBC promoting lymphocyte proliferation rates were significantly reduced after surgery. In addition, postoperative TNF-α, IL-2 and IFN-γ levels in RBC and lymphocyte culture fluid were lower than those pre-operation, whereas IL-10 and PGE2 were significantly increased compared to presurgical levels (p < 0.05).
  
  
  CONCLUSIONS
  The modification of RBC immune function may be involved in the occurrence and development of the infection following hip arthroplasty, and this suggests a novel strategy to prevent such infection.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Anhui, PR China.
authors.forename: Defu
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Anhui, PR China.
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Anhui, PR China.
authors.forename: Changma
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Anhui, PR China.
authors.forename: Runze
authors.initials: R
authors.lastname: Yu
keywords: immune function; red blood cell (RBC); total hip arthroplasty