title: Kindlin-2 interacts with α-actinin-2 and β1 integrin to maintain the integrity of the Z-disc in cardiac muscles.
abstract: Kindlin-2, as an integrin-interacting protein, was known to be required for the maintenance of cardiac structure and function in zebrafish. However, the mechanism remains unclear. We found that Kindlin-2 interacts and colocalizes with α-actinin-2 at the Z-disc of mouse cardiac muscles and there Kindlin-2 also interacts with β1 integrin. Knockdown of Kindlin-2 influences the association of β1 integrin with α-actinin-2 and disrupts the structure of the Z-disc and leads to cardiac dysfunction. Our data indicated that Kindlin-2 is a novel α-actinin-2-interacting protein and plays an important role in the regulation of cardiac structure and function.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Qi
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Chi
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Xu
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Danyu
authors.initials: D
authors.lastname: Lu
authors.affiliation: Institute for Cardiovascular Research, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Song
authors.affiliation: Institute for Cardiovascular Research, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China. Electronic address: Hongquan.Zhang@bjmu.edu.cn.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000185:Actinin / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism*; D008297:Male; D008813:Mice, Inbred ICR; D009124:Muscle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D021381:Protein Transport; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism* / Q000648:ultrastructure; D013318:Stroke Volume; D014463:Ultrasonography; D018754:Ventricular Dysfunction / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism
chemical_list: C498529:Actn2 protein, mouse; C498530:Actn2 protein, rat; D003598:Cytoskeletal Proteins; D019012:Integrin beta1; D009124:Muscle Proteins; D011993:Recombinant Fusion Proteins; C527802:kindlin-2 protein, mouse; D000185:Actinin; D005982:Glutathione Transferase
keywords: Cardiac structure; Kindlin-2; The Z-disc; α-Actinin-2