title: Modeling and estimation of production rate for the production phase of non-growth-associated high cell density processes.
abstract: This paper addresses the estimation of the specific production rate of intracellular products and the modeling of the bioreactor volume dynamics in high cell density fed-batch reactors. In particular, a new model for the bioreactor volume is proposed, suitable to be used in high cell density cultures where large amounts of intracellular products are stored. Based on the proposed volume model, two forms of a high-order sliding mode observer are proposed. Each form corresponds to the cases with residual biomass concentration or volume measurement, respectively. The observers achieve finite time convergence and robustness to process uncertainties as the kinetic model is not required. Stability proofs for the proposed observer are given. The observer algorithm is assessed numerically and experimentally.
authors.affiliation: Group of Control Applications, LEICI, Faculty of Engineering, La Plata National University, CONICET, C.C.91 (1900), La Plata, Argentina. martin.jamilis@ing.unlp.edu.ar.
authors.forename: Martín
authors.identifier: http://orcid.org/0000-0002-7101-2661
authors.initials: M
authors.lastname: Jamilis
authors.affiliation: Group of Control Applications, LEICI, Faculty of Engineering, La Plata National University, CONICET, C.C.91 (1900), La Plata, Argentina.
authors.forename: Fabricio
authors.initials: F
authors.lastname: Garelli
authors.affiliation: Department of Biosystems Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium.|Business Unit Separation and Conversion Technology, Flemish Institute for Technological Research (VITO), Boeretang 200, 2400, Mol, Belgium.
authors.forename: Md Salatul Islam
authors.initials: MS
authors.lastname: Mozumder
authors.affiliation: Research and Development Center for Industrial Fermentations (CINDEFI), School of Science, La Plata National University, CONICET, 47 and 115, B1900ASH, La Plata, Argentina.
authors.forename: Teresita
authors.initials: T
authors.lastname: Castañeda
authors.affiliation: Group of Control Applications, LEICI, Faculty of Engineering, La Plata National University, CONICET, C.C.91 (1900), La Plata, Argentina.
authors.forename: Hernán
authors.initials: H
authors.lastname: De Battista
mesh_terms: D058491:Bacterial Load / Q000379:methods*; D018407:Bacterial Physiological Phenomena; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D002452:Cell Count; D049109:Cell Proliferation / Q000502:physiology; D003198:Computer Simulation; D008954:Models, Biological
chemical_list: D002244:Carbon
keywords: Estimation; High cell density; Observer; Sliding mode; Volume model