title: Effect of carnosine, methylprednisolone and their combined application on irisin levels in the plasma and brain of rats with acute spinal cord injury.
abstract: Spinal cord injury (SCI) might occur to anybody at any time and any age. In its treatment, methylprednisolone (MP) is a first choice worldwide, but there is still no significant breakthrough in truly beneficial treatment due to SCI's complex pathophysiology. We investigated the effect of carnosine, methylprednisolone (MP) and its combination on irisin levels in the plasma, brain and medulla spinalis tissues in SCI using a rat model. The rats were divided into 6 groups: I (Control, saline); II (sham animals with laminectomy without cross-clamping); III (SCI); IV (SCI treated with 150mg/kg carnosine); V (SCI treated with 30mg/kg methylprednisolone); and VI (SCI treated with a combination of carnosine and MP). The animals were given traumatic SCI after laminectomy, using 70-g closing force aneurysm clips (Yasargil FE 721). Irisin concentration was measured by ELISA. The distribution of irisin in brain and spinal cord tissues was examined by immunochemistry. Irisin was mainly expressed in the astrocytes and microglia of brain tissues, and multipolar neurones of the anterior horn of spinal cord tissue in rats of all groups, indicating that irisin is physiologically indispensable. MP and carnosine and the combination of the two, significantly increased irisin in plasma and were accompanied by a significant rise in irisin immunoreactivity of brain and spinal cord tissues of the injured rats compared with control and sham. This finding raises the possibility that methylprednisolone and carnosine regulate the brain and spinal cord tissues in SCI by inducing irisin expression, and may therefore offer a better neurological prognosis.
authors.affiliation: Department of Neurosurgery, Elazig Education and Research Hospital, 23100, Elazig, Turkey.
authors.forename: Serdal
authors.initials: S
authors.lastname: Albayrak
authors.affiliation: Department of Neurosurgery, Elazig Education and Research Hospital, 23100, Elazig, Turkey.
authors.forename: İbrahim Burak
authors.initials: İB
authors.lastname: Atci
authors.affiliation: Laboratory of Medical Biochemistry, Elazig Education and Research Hospital, Elazig 23100, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kalayci
authors.affiliation: Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormones Research Group), 23119 Elazig, Turkey.
authors.forename: Musa
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Firat University, School of Medicine, Department of Histology&Embryology, 23119, Elazig, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kuloglu
authors.affiliation: Cardiovascular Surgery- Anatomy, Elazig Education and Research Hospital, 23100, Elazig, Turkey.
authors.forename: Suna
authors.initials: S
authors.lastname: Aydin
authors.affiliation: Firat University, Veterinary of Medicine, Department of Surgery, Elazig 23119, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kom
authors.affiliation: Department of Neurosurgery, Elazig Education and Research Hospital, 23100, Elazig, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Ayden
authors.affiliation: Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormones Research Group), 23119 Elazig, Turkey. Electronic address: saydin1@hotmail.com.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Aydin
mesh_terms: D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002336:Carnosine / Q000008:administration & dosage*; D005353:Fibronectins / Q000097:blood / Q000378:metabolism*; D007796:Laminectomy; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control
chemical_list: C583676:FNDC5 protein, rat; D005353:Fibronectins; D002336:Carnosine; D008775:Methylprednisolone
keywords: Carnosine; Irisin; Methylprednisolone; Spinal cord injury