title: Live imaging of β-1,3-glucan synthase FKS-1 in Neurospora crassa hyphae.
abstract: The subcellular localization and dynamics of FKS-1, the putative catalytic subunit of the β-1,3-glucan synthase complex, was analyzed in growing hyphae of Neurospora crassa by live confocal microscopy. GFP-tagged FKS-1 accumulated at the outer layer of the Spitzenkörper (Spk), and at the apical plasma membrane (PM). Fluorescence recovery after photobleaching analysis revealed arrival of FKS-1-containing carriers first at the immediate surroundings of the core region of the Spk, and thereafter to the Spk most outer region. The results obtained here and previous data suggest that FKS-1 is transported to the Spk in macrovesicles.
authors.affiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Baja California 22760, Mexico.
authors.forename: Eddy
authors.initials: E
authors.lastname: Sánchez-León
authors.affiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Baja California 22760, Mexico. Electronic address: riquelme@cicese.mx.
authors.forename: Meritxell
authors.initials: M
authors.lastname: Riquelme
mesh_terms: D005964:Glucosyltransferases / Q000378:metabolism*; D025301:Hyphae / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D014741:Video Recording; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D047071:beta-Glucans; C033363:beta-1,3-glucan; D005964:Glucosyltransferases; C040232:glucan synthase
keywords: Cell wall; FKS-1; Spitzenkörper