title: Metallome of Pseudomonas aeruginosa: a role for siderophores.
abstract: In this paper, we describe the total metal composition (metallome) of Pseudomonas aeruginosa. Inductively coupled plasma atomic emission spectroscopy analyses showed that P. aeruginosa cells concentrate each metal of the metallome from the extracellular media with different efficiencies. Growth in nutrient-restricted media did not substantially affect the overall profile of the metallome; however, the uptake of some metals was strongly stimulated, showing the high potential of some metal acquisition pathways to adapt to changing growth conditions. We also investigated the role of the two major siderophores produced by P. aeruginosa, pyoverdine and pyochelin, in iron uptake and more generally in metallome homeostasis. In addition to their role in iron acquisition, siderophore production also significantly prevented the accumulation of toxic metals in P. aeruginosa cells, thus preserving the equilibrium of the metallome in a polluted environment.
authors.affiliation: Université de Strasbourg, ESBS, Blvd Sébastien Brant, Illkirch, F-67413, France.|CNRS, UMR7242, Blvd Sébastien Brant, Illkirch, F-67413, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Cunrath
authors.affiliation: Université de Strasbourg, ESBS, Blvd Sébastien Brant, Illkirch, F-67413, France.|CNRS, UMR7242, Blvd Sébastien Brant, Illkirch, F-67413, France.
authors.forename: Valérie A
authors.initials: VA
authors.lastname: Geoffroy
authors.affiliation: Université de Strasbourg, ESBS, Blvd Sébastien Brant, Illkirch, F-67413, France. isabelle.schalk@unistra.fr.|CNRS, UMR7242, Blvd Sébastien Brant, Illkirch, F-67413, France. isabelle.schalk@unistra.fr.
authors.forename: Isabelle J
authors.initials: IJ
authors.lastname: Schalk
mesh_terms: D001693:Biological Transport, Active; D008670:Metals / Q000378:metabolism* / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*; D013054:Spectrophotometry, Atomic; D013844:Thiazoles / Q000378:metabolism*
chemical_list: D008670:Metals; D009842:Oligopeptides; D010636:Phenols; D017262:Siderophores; D013844:Thiazoles; C025316:pyochelin; C042453:pyoverdin