title: The efficacy of chimeric vaccines constructed with PEP-1 and Ii-Key linking to a hybrid epitope from heterologous viruses.
abstract: The heterologous epitope-peptide from different viruses may represent an attractive candidate vaccine. In order to evaluate the role of cell-permeable peptide (PEP-1) and Ii-Key moiety from the invariant chain (Ii) of MHC on the heterologous peptide chimeras, we linked the two vehicles to hybrid epitopes on the VP2 protein (aa197-209) of the infectious bursal disease virus and HN protein (aa345-353) of the Newcastle disease virus. The chimeric vaccines were prepared and injected into mice. The immune effects were measured by indirect ELISA. The results showed that the vehicle(s) could significantly boost immune effects against the heterologous epitope peptide. The Ii-Key-only carrier induced more effective immunological responses, compared with the PEP-1 and Ii-Key hybrid vehicle. The carrier-peptide hybrids all showed strong colocalization with major histocompatibility complex (MHC) class II molecules compared with the epitope-peptide (weakly-binding) after co-transfection into 293T cells. Together, our results lay the groundwork for designing new hybrid vaccines based on Ii-Key and/or PEP-1 peptides.
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, 130 Changjiang Ave, Hefei 230036, Anhui, China. Electronic address: liuxuelan203@163.com.
authors.forename: Xue-lan
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, 130 Changjiang Ave, Hefei 230036, Anhui, China.
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Shan
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, 130 Changjiang Ave, Hefei 230036, Anhui, China.
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Xu
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, 130 Changjiang Ave, Hefei 230036, Anhui, China.
authors.forename: Jin-jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, 130 Changjiang Ave, Hefei 230036, Anhui, China.
authors.forename: Fa-zhi
authors.initials: FZ
authors.lastname: Xu
authors.affiliation: Center of Animal Epidemic Disease Prevention and Control, 460 Liming Ave, Wuhu 241000, Anhui, China.
authors.forename: Sheng-lin
authors.initials: SL
authors.lastname: Xia
authors.affiliation: Anhui Animal Husbandry and Veterinary Research Institute, Anhui Academy of Agricultural Sciences, 40 Nongke Ave, Hefei 230031, Anhui, China. Electronic address: daiyin2020@163.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000276:immunology; D000939:Epitopes / Q000737:chemistry*; D057809:HEK293 Cells; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D009522:Newcastle disease virus / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology*; D014765:Viral Vaccines / Q000737:chemistry / Q000276:immunology*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; C503924:Pep-1 peptide; D010455:Peptides; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C045084:invariant chain; D003543:Cysteamine
keywords: Heterologous epitope-peptide; Ii-Key; PEP-1; The vehicle effect; Vaccine