title: Cross-reactivity of a new food ingredient, dun pea, with legumes, and risk of anaphylaxis in legume allergic children.
abstract: BACKGROUND
  Legume allergy is the fifth food allergy in Europe. The dun pea (Pisum sativum sativum var. arvense), a pea belonging to the same subspecies as green pea, has been recently introduced as an ingredient in the human food industry. The aims of this study were to evaluate the cross-reactivity between dun pea and other legumes and to search for modification of allergenicity induced by food technologies.
  
  
  METHODS
  A series of 36 patients with legume and/or peanut allergy was studied. They underwent skin tests to peanut and a panel of legumes including dun pea. Specific IgE to dun pea and cross-reactivity to peanut allergens, particularly to Ara h 1, were evaluated by ELISA. Proteins and allergens of different pea extracts were studied by SDS-PAGE and immunoblots.
  
  
  RESULTS
  In France and Belgium, 7.7% of severe food anaphylaxis cases were due to legumes. Patients with isolated legume allergy had positive prick tests to dun pea, whereas patients with isolated peanut allergy had negative prick tests. Cross-reactivity between sIgE to peanut and dun pea was observed, and more frequently than expected (96%) peanut-allergic patients with legume sensitization or allergy had sIgE to Ara h 1. Analysis of dun pea allergens suggested that protein epitopes were presented differently in dun pea seeds, isolate and flour.
  
  
  CONCLUSIONS
  This study identifies, for the first time, a risk of dun pea allergy in legume-allergic patients and in a subset of peanut-allergic patients.
authors.affiliation: Genclis SAS, Vandoeuvre-Lès-Nancy, France. Genclis sas, 15 rue du bois de la Champelle 54500 Vandoeuvre-Lès-Nancy Cedex, France. E-mail: c.richard@genclis.com.
authors.forename: C
authors.initials: C
authors.lastname: Richard
authors.affiliation: Genclis SAS, Vandoeuvre-Lès-Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Jacquenet
authors.affiliation: Internal Medicine, Clinical Immunology and Allergology, Nancy University Hospital, France.
authors.forename: P
authors.initials: P
authors.lastname: Sergeant
authors.affiliation: Lorraine-Nancy University, Durkheim Hospital, Epinal, France. AllergyVigilance Network, Vandoeuvre-Lès-Nancy, France.
authors.forename: D A
authors.initials: DA
authors.lastname: Moneret-Vautrin
mesh_terms: D000293:Adolescent; D000707:Anaphylaxis / Q000209:etiology*; D010367:Arachis / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D007887:Fabaceae / Q000276:immunology*; D005260:Female; D005512:Food Hypersensitivity / Q000209:etiology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D012306:Risk; D012882:Skin Tests
chemical_list: D007073:Immunoglobulin E
keywords: Cross-reactivity; dun pea; legume allergy; peanut allergy; specific IgE