title: The complete mitochondrial genome of the endemic and threatened killifish Orestias ascotanensis Parenti, 1984 (Cyprinodontiformes, Cyprinodontidae) from the High Andes.
abstract: The killifish Orestias ascotanensis is endemic to the small isolated springs of Ascotán salt pan in the Central High Andes, Chile. Due to small populations, mining activity, and increasing aridity, this species is catalogued in danger of extinction. The complete mitochondrial genome of O. ascotanesis was assembled with an Ion Torrent sequencer (chip 318) that produced 2.61 million of reads. The 16 617 bp of the entire genome consisted of 22 transfer RNAs, 2 ribosomal RNAs, 13 protein-coding genes, and a control region, showing that the gene composition and arrangement match to that reported for most fishes.
authors.affiliation: a Fish and Isotope Ecology Laboratory , Instituto de Ciencias Naturales Alexander von Humboldt, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta , Antofagasta , Chile .
authors.forename: Claudio
authors.initials: C
authors.lastname: Quezada-Romegialli
authors.affiliation: b Departamento de Ciencias Ecológicas , Universidad de Chile , Santiago , Chile .|c Escuela de Medicina Veterinaria sede Talca, Facultad de Recursos Naturales y Medicina Veterinaria, Universidad Santo Tomás , Talca , Chile , and.
authors.forename: Claudia Jimena
authors.initials: CJ
authors.lastname: Guerrero
authors.affiliation: b Departamento de Ciencias Ecológicas , Universidad de Chile , Santiago , Chile .|d Instituto de Ecología y Biodiversidad, Núcleo Milenio de Ecología y Manejo Sustentable de Islas Oceánicas, Universidad de Chile , Santiago , Chile.
authors.forename: David
authors.initials: D
authors.lastname: Véliz
authors.affiliation: b Departamento de Ciencias Ecológicas , Universidad de Chile , Santiago , Chile .
authors.forename: Irma
authors.initials: I
authors.lastname: Vila
mesh_terms: D000818:Animals; D005618:Fresh Water; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D007695:Killifishes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Altiplano; NGS; freshwater fish; molecular phylogenetics; next-generation sequencing