title: Isorhamnetin Protects Human Keratinocytes against Ultraviolet B-Induced Cell Damage.
abstract: Isorhamnetin (3-methylquercetin) is a flavonoid derived from the fruits of certain medicinal plants. This study investigated the photoprotective properties of isorhamnetin against cell damage and apoptosis resulting from excessive ultraviolet (UV) B exposure in human HaCaT keratinocytes. Isorhamnetin eliminated UVB-induced intracellular reactive oxygen species (ROS) and attenuated the oxidative modification of DNA, lipids, and proteins in response to UVB radiation. Moreover, isorhamnetin repressed UVB-facilitated programmed cell death in the keratinocytes, as evidenced by a reduction in apoptotic body formation, and nuclear fragmentation. Additionally, isorhamnetin suppressed the ability of UVB light to trigger mitochondrial dysfunction. Taken together, these results indicate that isorhamnetin has the potential to protect human keratinocytes against UVB-induced cell damage and death.
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Xia
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Ki Cheon
authors.initials: KC
authors.lastname: Kim
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Susara Ruwan Kumara
authors.initials: SR
authors.lastname: Madduma Hewage
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Yoo
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.affiliation: Department of Mechanical Engineering & Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701.
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Shin
authors.affiliation: National Fusion Research Institute, Plasma Technology Research Center, Gunsan 573-540.
authors.forename: Yeunsoo
authors.initials: Y
authors.lastname: Park
authors.affiliation: National Fusion Research Institute, Plasma Technology Research Center, Gunsan 573-540.
authors.forename: Suk Jae
authors.initials: SJ
authors.lastname: Yoo
authors.affiliation: Aging Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
keywords: Human keratinocyte; Isorhamnetin; Programmed cell death; Reactive oxygen species; Ultraviolet B