title: The Diagnostic Value of Plasma SCUBE1, a Novel Biomarker of Platelet Activation, in Testicular Torsion: A Randomized, Controlled, Experimental Study.
abstract: OBJECTIVE
  To investigate the potential diagnostic value of plasma signal peptide, CUB (complement proteins C1r/C1s, Uegf, Bmp1) domain, epidermal growth factor (EGF)-like 1 (SCUBE1) protein in experimentally induced testicular torsion (TT).
  
  
  METHODS
  In this randomized, controlled, experimental study, 24 mature male Wistar rats were divided into four groups: 2- and 4-hour control (groups I and III, respectively), and 2- and 4-hour torsion (groups II and IV, respectively) groups. Torsion was performed by rotating the left testis 720° clockwise and maintained by fixing the testis. Plasma SCUBE1 levels and histopathological damage scores were compared.
  
  
  RESULTS
  There was significantly greater histopathological damage in the 4-hour torsion group compared with the other groups. SCUBE1 levels in this group were also higher than those in the other groups, and the difference was significant. There were significant correlations between histopathological scores and SCUBE1 levels.
  
  
  CONCLUSIONS
  SCUBE1, a novel marker of platelet activation, is elevated in TT. According to our results, platelet activation may play an important pathological role in tissue injury associated with testicular ischemia. Plasma SCUBE1 measurement may have diagnostic, therapeutic, or prognostic value in TT.
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. Electronic address: suleymanturedi@hotmail.com.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Turedi
authors.affiliation: Department of Emergency Medicine, Kanuni Training and Research Hospital, Trabzon, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Tatli
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
authors.affiliation: Department of Clinical Urology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Ersagun
authors.initials: E
authors.lastname: Karaguzel
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Yunus
authors.initials: Y
authors.lastname: Karaca
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Suha
authors.initials: S
authors.lastname: Turkmen
authors.affiliation: Department of Histology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Esin
authors.initials: E
authors.lastname: Yulug
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Sumer
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Diler Us
authors.initials: DU
authors.lastname: Altay
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Mentese
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002352:Carrier Proteins / Q000097:blood*; D004195:Disease Models, Animal; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D015539:Platelet Activation / Q000502:physiology*; D011237:Predictive Value of Tests; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013086:Spermatic Cord Torsion / Q000097:blood* / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D008565:Membrane Proteins; C528568:Scube1 protein, rat