title: Human unrestricted somatic stem cells loaded in nanofibrous PCL scaffold and their healing effect on skin defects.
abstract: Unrestricted somatic stem cells (USSCs) loaded in nanofibrous polycaprolactone (PCL) scaffolds can be used for skin regeneration when grafted onto full-thickness skin defects of rats. Nanofibrous PCL scaffolds were designed by the electrospinning method and crosslinked with laminin protein. Afterwards, the scaffolds were evaluated by scanning electron microscopy, and physical and mechanical assays. In this study, nanofibrous PCL scaffolds loaded with USSCs were grafted onto the skin defects. The wounds were subsequently investigated 21 days after grafting. Results of mechanical and physical analyses showed good resilience and compliance to movement as a skin graft. In animal models; study samples exhibited the most pronounced effect on wound closure, with statistically significant improvement in wound healing being seen at 21 days post-operatively. Histological examinations of healed wounds from all samples showed a thin epidermis plus recovered skin appendages in the dermal layer for samples with cell. Thus, the graft of nanofibrous PCL scaffolds loaded with USSC showed better results during the healing process of skin defects in rat models.
authors.affiliation: a Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Hoda
authors.initials: H
authors.lastname: Bahrami
authors.affiliation: a Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences , Tehran , Iran.|b Proteomics Research Center, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Saeed Heidari
authors.initials: SH
authors.lastname: Keshel
authors.affiliation: c Faculty of Medical Sciences, Guilan University of Medical Sciences , Guilan , Iran.
authors.forename: Aliakbar Jafari
authors.initials: AJ
authors.lastname: Chari
authors.affiliation: d Department of Biomaterials Engineering , Tonekabon Branch, Islamic Azad University , Tonekabon , Iran.
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Biazar
mesh_terms: D000818:Animals; D064593:Heterografts; D006801:Humans; D008297:Male; D057139:Nanofibers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D017768:Skin, Artificial; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
keywords: nanofibrous pcl scaffold; rat; unrestricted somatic stem cells; wound healing