title: Comparison of the artus HIV-1 QS-RGQ and VERSANT HIV-1 RNA 1.0 assays for quantitative detection of human immunodeficiency virus type 1 in plasma samples.
abstract: Several integrated diagnostic platforms to quantify human immunodeficiency virus type-1 viremia have been developed in recent years. We evaluated the performances of the Artus HIV-1 QS-RGQ assay, using the complete QIAsymphony RGQ workflow. 192 clinical plasma specimens and external control panel samples were analyzed, using the Artus assay and the routine Siemens VERSANT HIV-1 RNA 1.0 assay. Three samples were excluded due to amplification inhibition. Among the remaining 189 specimens, 130 samples were detected as positive (above the limit of detection by both assays; median log10 difference: 0.01) and 18 samples were detected as negative. Eight samples (4.2%), all slightly above the limit of detection of the Versant assay, were negative with the Artus assay. The remaining 33 samples (beside 3 negative by Artus assay) were positive by both assays, but below the limit of detection at least in one of them. Results from the external panel samples showed a mean Log10 variation of -0.18 and -0.45 for the Versant and the Artus assays, respectively. As both assays showed highly correlated results, the QIAsymphony RGQ system, using the Artus HIV-1 QS-RGQ assay, could be considered a potential platform for HIV-1 RNA quantification in plasma.
authors.affiliation: Laboratory of Microbiology, Hospital San Raffaele IRCCS, Milan Italy.
authors.forename: Roee
authors.initials: R
authors.lastname: Dvir
authors.affiliation: Laboratory of Microbiology, Hospital San Raffaele IRCCS, Milan Italy.|University of Insubria, Dept. of Biotechnology and Life Sciences, Varese, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Canducci
authors.affiliation: Laboratory of Microbiology, Hospital San Raffaele IRCCS, Milan Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Racca
authors.affiliation: Laboratory of Microbiology, Hospital San Raffaele IRCCS, Milan Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Rolla
authors.affiliation: Laboratory of Microbiology, Hospital San Raffaele IRCCS, Milan Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Stucchi
authors.affiliation: Laboratory of Microbiology, Hospital San Raffaele IRCCS, Milan Italy.|University Vita-Salute San Raffaele, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Clementi
mesh_terms: D015658:HIV Infections / Q000175:diagnosis / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D019562:Viral Load
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic
keywords: Artus HIV-1 QS-RGQ assay; Human immunodeficiency virus type-1 (HIV-1); Quantitative real-time polymerase chain reaction (qPCR)