title: [Comparison of chemotaxis of different concentrations of SDF1,bFGF,IL-8 on bone marrow mesenchymal stem cell].
abstract: OBJECTIVE
  To investigate chemotaxis of different concentrations of chemokines stromal cell-derived factor-1 (SDF-1), basic fibroblast growth factor (bFGF) and interleukin-8 (IL-8) on bone marrow mesenchymal stem cells (BMSCs).
  
  
  METHODS
  The experiment group was divided into 3 groups according to different culture media: SDF1, bFGF and IL-8. The chemokine concentrations of each group was 0 ng/mL (control concentration),10 ng/mL, 50 ng/mL, 100 ng/mL, 200ng/mL and 400 ng/mL (experimental concentration). BMSCs were isolated from SD rats and cultured in vitro. 24-well plates with an 8 μm pore size polycarbonate membrane were used to examine the chemotaxis of SDF1, bFGF, IL-8 on BMSCs, respectively, and to obtain the best chemotactic concentrations of 3 chemokines. The data was analyzed with SPSS 16.0 software package.
  
  
  RESULTS
  Compared with 0 ng/mL group, all chemokines could chemoattract BMSCs (P<0.05). At the concentration of 50 ng/mL, 100 ng/mL and 400 ng/mL, SDF1 significantly recruited more cells than bFGF (P<0.05); SDF1 significantly recruited more cells than IL-8 at the concentration of 200 ng/mL; The best chemotactic concentration was 100 ng/mL for SDF1 and IL-8 and 200 ng/mL for bFGF.
  
  
  CONCLUSIONS
  The use of SDF1,bFGF,IL-8 can chemoattract BMSCs. The chemotaxis of SDF1 and IL-8 are better than bFGF. Supported by Medical Guide Project of Science and Technology Committee of Shanghai Municipality (124119b0101).
authors.affiliation: Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:yujianyuexing@126.com.
authors.forename: Yu-jian
authors.initials: YJ
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Zheng-bin
authors.initials: ZB
authors.lastname: Chi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D002633:Chemotaxis; D016222:Fibroblast Growth Factor 2; D016209:Interleukin-8; D059630:Mesenchymal Stem Cells; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; D016209:Interleukin-8; D016222:Fibroblast Growth Factor 2