title: HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
abstract: A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots. Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis. The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %. Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR). The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group. PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group. The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla. As a result, AR possessed a similar immunoregulation function as NR.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Hongfa
authors.initials: H
authors.lastname: Li
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wu
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D001688:Biological Products / Q000276:immunology*; D019149:Bioreactors; D053898:Biosynthetic Pathways; D029748:Caryophyllaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000473:pathology; D051379:Mice; D010456:Peptides, Cyclic / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D001688:Biological Products; D010456:Peptides, Cyclic; D011134:Polysaccharides; D012503:Saponins