title: Error filtering, pair assembly and error correction for next-generation sequencing reads.
abstract: BACKGROUND
  Next-generation sequencing produces vast amounts of data with errors that are difficult to distinguish from true biological variation when coverage is low.
  
  
  RESULTS
  We demonstrate large reductions in error frequencies, especially for high-error-rate reads, by three independent means: (i) filtering reads according to their expected number of errors, (ii) assembling overlapping read pairs and (iii) for amplicon reads, by exploiting unique sequence abundances to perform error correction. We also show that most published paired read assemblers calculate incorrect posterior quality scores.
  
  
  METHODS
  These methods are implemented in the USEARCH package. Binaries are freely available at http://drive5.com/usearch.
  
  
  BACKGROUND
  robert@drive5.com
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Tiburon, CA 94920, USA and.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Edgar
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Flyvbjerg
mesh_terms: D000465:Algorithms; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D012984:Software