title: Correlation analysis of gene polymorphisms and β-lactam allergy.
abstract: A total of 64 patients with β-lactam allergy and 30 control subjects were enrolled in a case-control study. This study is aimed to analyze the relationship between β-lactam allergy and 10 single nucleotide polymorphisms (SNPs) in interleukin-10 (IL-10), IL-13, IL-4Rα, high-affinity immunoglobulin E-receptor β chain (FcεRIβ), interferon γ receptor 2 (IFNGR2), and CYP3A4, and within the Han Chinese population of Northwest China. Genotyping for the SNPs was conducted using the Sequenom MassARRAY(®) platform. SPSS 17.0 was employed to analyze the statistical data and SHEsis was used to perform the haplotype reconstruction and analyze linkage disequilibrium of SNPs of IL-10 and IL-13. The results showed that the genotype distribution of CYP3A4 rs2242480/CT differed significantly between case and control groups of males (P=0.022; odds ratio (OR)=0.167, 95% confidence interval (CI): 0.032-0.867). Further analysis showed that CCA, CCG, and TAA haplotypes of IL-10 had no significant correlation in patients with β-lactam allergy. The correlation between CCT and CAC haplotypes of IL-13 and β-lactam allergy needs to be further studied. The analysis did not reveal any differences in the distribution of others gene polymorphisms between cases and controls.
authors.affiliation: Centre of Laboratory Medicine, Lanzhou University Second Hospital, Lanzhou 730030, China; School of Basic Medical Science, Lanzhou University, Lanzhou 730030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xin-yue
authors.initials: XY
authors.lastname: Liu
authors.forename: Lin-jing
authors.initials: LJ
authors.lastname: Li
authors.forename: Chong-ge
authors.initials: CG
authors.lastname: You
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Shang-di
authors.initials: SD
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Ze-jing
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Ting-hong
authors.initials: TH
authors.lastname: Lv
mesh_terms: D000328:Adult; D004342:Drug Hypersensitivity / Q000209:etiology / Q000235:genetics* / Q000276:immunology*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D018793:Interleukin-13 / Q000235:genetics* / Q000276:immunology*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013223:Statistics as Topic; D047090:beta-Lactams / Q000009:adverse effects*
chemical_list: C508609:IL10 protein, human; C578095:IL13 protein, human; D018793:Interleukin-13; D047090:beta-Lactams; D016753:Interleukin-10
keywords: Allergy; Interleukin (IL); Pharmacogenomics; Single nucleotide polymorphism (SNP); β-Lactam