title: Influence of Dietary Selenium Species on Selenoamino Acid Levels in Rainbow Trout.
abstract: Two forms of selenium (Se) supplementation of fish feeds were compared in two different basal diets. A 12-week feeding trial was performed with rainbow trout fry using either a plant-based or a fish meal-based diet. Se yeast and selenite were used for Se supplementation. Total Se and Se speciation were determined in both diets and whole body of trout fry using inductively coupled plasma mass spectrometry (ICP MS) and high-performance liquid chromatography (HPLC). The two selenoamino acids, selenomethionine (SeMet) and selenocysteine (SeCys), were determined in whole body of fry after enzymatic digestion using protease type XIV with a prior derivatization step in the case of SeCys. The plant-based basal diet was found to have a much lower total Se than the fish meal-based basal diet with concentrations of 496 and 1222 μg(Se) kg(-1), respectively. Dietary Se yeast had a higher ability to raise whole body Se compared to selenite. SeMet concentration in the fry was increased only in the case of Se yeast supplementation, whereas SeCys levels were similar at the end of the feeding trial for both Se supplemented forms. The results show that the fate of dietary Se in fry is highly dependent on the form brought through supplementation and that a plant-based diet clearly benefits from Se supplementation.
authors.affiliation: †LCABIE, UMR 5254 CNRS, 2 Avenue Pierre Angot, F-64053 Pau Cedex 09, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Godin
authors.affiliation: §INRA, UR1067 NuMéA, Route Départementale 918, F-64310 Saint-Pée-sur-Nivelle, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Fontagné-Dicharry
authors.affiliation: †LCABIE, UMR 5254 CNRS, 2 Avenue Pierre Angot, F-64053 Pau Cedex 09, France.
authors.forename: Maïté
authors.initials: M
authors.lastname: Bueno
authors.affiliation: #Lesaffre Feed Additives, 137 Rue Gabriel Péri, F-59700 Marcq-en-Barœul, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Tacon
authors.affiliation: §INRA, UR1067 NuMéA, Route Départementale 918, F-64310 Saint-Pée-sur-Nivelle, France.
authors.forename: Philip Antony Jesu
authors.initials: PA
authors.lastname: Prabhu
authors.affiliation: §INRA, UR1067 NuMéA, Route Départementale 918, F-64310 Saint-Pée-sur-Nivelle, France.
authors.forename: Sachi
authors.initials: S
authors.lastname: Kaushik
authors.affiliation: §INRA, UR1067 NuMéA, Route Départementale 918, F-64310 Saint-Pée-sur-Nivelle, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Médale
authors.affiliation: †LCABIE, UMR 5254 CNRS, 2 Avenue Pierre Angot, F-64053 Pau Cedex 09, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Bouyssiere
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D005396:Fish Products; D013058:Mass Spectrometry; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010944:Plants; D020887:Selenious Acid / Q000008:administration & dosage; D012643:Selenium / Q000008:administration & dosage*; D017279:Selenocysteine / Q000032:analysis*; D012645:Selenomethionine / Q000032:analysis*
chemical_list: D017279:Selenocysteine; D012645:Selenomethionine; D020887:Selenious Acid; D012643:Selenium
keywords: fish; selenium speciation; selenocysteine; selenomethionine; supplementation