title: Identification of Paenibacillus as a Symbiont in Acanthamoeba.
abstract: Amoebae of the genus Acanthamoeba occur worldwide and in addition to being pathogens, are important vehicles for microorganisms with clinical and environmental importance. This study aimed to evaluate the profiling of endosymbionts in 12 isolates of Acanthamoeba using V3 region of 16S rDNA denaturing gradient gel electrophoresis (DGGE) and sequencing. The DGGE enabled us to characterize the endosymbionts diversity in isolates of Acanthamoeba, and to identify Paenibacillus sp., an emerging pathogen, as an amoebic endosymbiont. The results of this study demonstrated that Acanthamoeba is capable of transporting a large number of endosymbionts. This is the first study that reports, the presence of Paenibacillus sp. as amebic symbiont.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Vinicius José
authors.initials: VJ
authors.lastname: Maschio
authors.forename: Gertrudes
authors.initials: G
authors.lastname: Corção
authors.forename: Francielle
authors.initials: F
authors.lastname: Bücker
authors.forename: Karin
authors.initials: K
authors.lastname: Caumo
authors.forename: Marilise Brittes
authors.initials: MB
authors.lastname: Rott
mesh_terms: D000048:Acanthamoeba / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000302:isolation & purification* / Q000502:physiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S