title: [Composites of Peptide Nucleic Acids with Titanium Dioxide Nanoparticles. IV+. Antiviral Activity of Nanocomposites Containing DNA/PNA Duplexes].
abstract: Antiviral activity of TiO2 · PL · DNA/PNA nanobiocomposites was studied on the MDCK cell culture infected with influenza A virus (subtype H3N2). PNA fragment in nanocomposites as a DNA/PNA heteroduplex is electrostatically bound to titanium dioxide nanoparticles precovered with polylysine (TiO2 · PL). It was shown that TiO2 · PL · DNA1/PNA1 nanobiocomposit bearing PNA1 fragment targeted to the 3'-end of the noncoding region of segment 5 of viral RNA specifically inhibited the virus reproduction with the efficiency of 99.8%. It was determined that the 50% cytotoxic concentration (TC50) of the TiO2 · PL · DNA1/PNA1 nanocomposite is more than 1200 mg/mL. And 50% effective inhibitory concentration (IC50) is less than 0.003 mg/mL. Based on these data, the selectivity index (SI) for TiO2 · PL · DNA1/PNA1 nanobiocomposite defined as the ratio TC50/LC50, is more than 400. Thus TiO2 · PL · DNA/PNA nanobiocomposites can not only penatrate through cell membrane, but and are able to exhibit a high specific antisense activity, without causing toxic effects on the living cells.
authors.forename: R N
authors.initials: RN
authors.lastname: Amirkhanov
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: N V
authors.initials: NV
authors.lastname: Amirkhanov
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D004285:Dogs; D009980:Influenza A virus / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D053761:Nanocomposites / Q000737:chemistry*; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D020135:Peptide Nucleic Acids; C009495:titanium dioxide; D004247:DNA; D014025:Titanium