title: Calcitriol Suppressed Inflammatory Reactions in IL-1β-Stimulated Human Periodontal Ligament Cells.
abstract: Vitamin D has important roles on control of calcium and phosphate levels in the body. However, the role of vitamin D on the pathogenesis of periodontal disease is still uncertain. Therefore, we examined the effect of the hormonal form of vitamin D, calcitriol, on inflammatory responses of human periodontal ligament cells (HPDLC). We detected vitamin D receptor expression in non-stimulated HPDLC. Calcitriol inhibited interleukin (IL)-6, IL-8, CC chemokine ligand (CCL) 20, CXC chemokine ligand (CXCL) 10, and matrix metalloproteinase (MMP)-3 release from IL-1β-stimulated HPDLC. Tissue inhibitor of metalloproteinase (TIMP)-1 production did not change by calcitriol. Moreover, we found c-jun N-terminal kinase (JNK) phosphorylation and IκB-α degradation in IL-1β-stimulated HPDLC were inhibited by calcitriol, and JNK and nuclear factor (NF)-κB inhibitors could decrease IL-6, IL-8, CCL20, CXCL10, and MMP-3 productions in IL-1β-treated HPDLC. These findings suggest that vitamin D could modulate inflammatory response in periodontal tissues.
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan. hosokawa@tokushima-u.ac.jp.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hosokawa
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Hosokawa
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Shindo
authors.affiliation: Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Ozaki
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D054418:Chemokine CCL20 / Q000378:metabolism; D054357:Chemokine CXCL10 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D010513:Periodontal Ligament / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010518:Periodontitis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D059748:Proteolysis; D018167:Receptors, Calcitriol / Q000819:agonists / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; C108355:CCL20 protein, human; C114753:CXCL10 protein, human; C508606:CXCL8 protein, human; D054418:Chemokine CCL20; D054357:Chemokine CXCL10; D020672:I-kappa B Proteins; C508585:IL1B protein, human; C508600:IL6 protein, human; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; C000606204:NFKBIA protein, human; D018167:Receptors, Calcitriol; C579457:VDR protein, human; D000072000:NF-KappaB Inhibitor alpha; D048031:JNK Mitogen-Activated Protein Kinases; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; D002117:Calcitriol
keywords: MAPKs; NF-κB; calcitriol; human periodontal ligament cells; inflammatory mediators