title: Antioxidant property of synbiotic combination of Lactobacillus sp. and wheat bran xylo-oligosaccharides.
abstract: Wheat bran water unextractable portion (WB-WUP) was subjected to xylanase treatment to obtain a mixture of xylo-oligosaccharides (XOS). XOS mixture was purified on charcoal-celite column and the individual oligosaccharides were separated on a Bio-Gel P-2 column. The sugar composition of the purified oligosaccharides was determined by GLC and their structure was deduced by ESI-MS and (1)H and (13)C NMR. The major oligosaccharides identified were xylobiose and xylotriose (consisting of arabinose). Five strains of lactobacilli (probiotics), XOS (prebiotics) and a combination of both (synbiotics) in milk (as medium) were monitored for antioxidant activity. DPPH radical scavenging activity (~70 %) as well as ferric reducing power (~80 mg/100 ml FeSO4eq) were significantly higher (p < 0.05) in all the synbiotic preparations compared to that of control. The present study indicated that the synbiotic preparations consisting of XOS and lactobacilli can be effectively used as dietary supplement.
authors.affiliation: Department of Biochemistry and Nutrition, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysore, 570020, Karnataka India.
authors.forename: Lyned D
authors.initials: LD
authors.lastname: Lasrado
authors.affiliation: Department of Biochemistry and Nutrition, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysore, 570020, Karnataka India.
authors.forename: Muralikrishna
authors.initials: M
authors.lastname: Gudipati
keywords: Antioxidant; Probiotic; Synbiotic; Xylo-oligosaccharides