title: Edaravone injection ameliorates cognitive deficits in rat model of Alzheimer's disease.
abstract: Oxidative stress plays important role in the pathogenesis of Alzheimer's disease (AD). Edaravone is a potent free radical scavenger that exerts antioxidant effects. Therefore, in this study we aimed to investigate neuroprotective effects of edaravone for AD. Wistar rats were randomly divided into three groups (n = 15): control group, model group, and treatment group, which were injected with phosphate buffered saline, Aβ1-40, and Aβ1-40 together with 5 mg/kg edaravone, respectively, into the right hippocampal dentate gyrus. Spatial learning and memory of the rats were examined by Morris water maze test. 4-Hydroxynonenal (4-HNE) level in rat hippocampus was analyzed by immunohistochemistry. Acetylcholinesterase (AChE) and choline acetylase (ChAT) activities were assayed by commercial kits. We found that edaravone ameliorated spatial learning and memory deficits in the rats. 4-HNE level in the hippocampus as well as AChE and ChAT activities in the hippocampus was significantly lower in treatment group than in model group. In conclusion, edaravone may be developed as a novel agent for the treatment of AD for improving cholinergic system and protecting neurons from oxidative toxicity.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Liaoning Medical University, No. 2, 5th Duan, People's Street, Guta District, Jinzhou, 121001, China. yangruijz@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Liaoning Medical University, Jinzhou, 121001, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Liaoning Medical University, No. 2, 5th Duan, People's Street, Guta District, Jinzhou, 121001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Liaoning Medical University, No. 2, 5th Duan, People's Street, Guta District, Jinzhou, 121001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Liaoning Medical University, No. 2, 5th Duan, People's Street, Guta District, Jinzhou, 121001, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Liu
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000523:psychology; D016229:Amyloid beta-Peptides; D000818:Animals; D000983:Antipyrine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D018891:Dentate Gyrus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D000077553:Edaravone; D007150:Immunohistochemistry; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D018697:Nootropic Agents / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments; D011897:Random Allocation; D017208:Rats, Wistar; D065852:Spatial Memory / Q000187:drug effects / Q000502:physiology
chemical_list: D000447:Aldehydes; D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D018697:Nootropic Agents; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; C027576:4-hydroxy-2-nonenal; D000077553:Edaravone; D000983:Antipyrine
keywords: 4-Hydroxynonenal; Alzheimer’s disease; Edaravone; Oxidative stress