title: Double-site recognition of pathogenic bacterial whole cells based on an antibiotic-affinity strategy.
abstract: An antibiotic-affinity strategy was designed to detect pathogenic bacterial whole cells based on the strong affinity of the antibiotic agent to bind to the cell wall of the bacterium. Vancocin, a powerful glycopeptide antibiotic, was used as a molecular recognition agent for Staphylococcus aureus. To improve its specificity, IgG was utilized as the second recognition agent based on the binding between the Fc region of IgG and protein A in the cell wall.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. fuzf@swu.edu.cn.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Gao
authors.forename: Shijia
authors.initials: S
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.forename: Guojian
authors.initials: G
authors.lastname: Liao
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001681:Biological Assay; D002473:Cell Wall / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008892:Milk / Q000382:microbiology; D044967:Serum / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D014640:Vancomycin