title: Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.
abstract: BACKGROUND
  This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.
  
  
  METHODS
  CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.
  
  
  RESULTS
  The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.
  
  
  CONCLUSIONS
  Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Song
authors.initials: S
authors.lastname: Feng
authors.affiliation: Department of Pathology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029 PR China.
authors.forename: Chuan-Tao
authors.initials: CT
authors.lastname: Yuan
authors.affiliation: Department of Pathology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029 PR China.
authors.forename: Ren-Ya
authors.initials: RY
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Guang-Kui
authors.initials: GK
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Gen-Hua
authors.initials: GH
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Xi-Zhen
authors.initials: XZ
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.
authors.forename: Ling-Sheng
authors.initials: LS
authors.lastname: Kong
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022 PR China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022 PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
keywords: Animal model; CD133; Cancer stem-like cell; Glioblastoma; Transplanted tumor