title: Production of technical-grade sodium citrate from glycerol-containing biodiesel waste by Yarrowia lipolytica.
abstract: The production of technical-grade sodium citrate from the glycerol-containing biodiesel waste by Yarrowia lipolytica was studied. Batch experiments showed that citrate was actively produced within 144 h, then citrate formation decreased presumably due to inhibition of enzymes involved in this process. In contrast, when the method of repeated batch cultivation was used, the formation of citrate continued for more than 500 h. In this case, the final concentration of citrate in the culture liquid reached 79-82 g/L. Trisodium citrate was isolated from the culture liquid filtrate by the addition of a small amount of NaOH, so that the pH of the filtrate increased to 7-8. This simple and economic isolation procedure gave the yield of crude preparation containing trisodium citrate 5.5-hydrate up to 82-86%.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Kamzolova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Natalia G
authors.initials: NG
authors.lastname: Vinokurova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Julia N
authors.initials: JN
authors.lastname: Lunina
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Nina F
authors.initials: NF
authors.lastname: Zelenkova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia. Electronic address: morgunovs@rambler.ru.
authors.forename: Igor G
authors.initials: IG
authors.lastname: Morgunov
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D000077559:Sodium Citrate; D025062:Yarrowia / Q000502:physiology*
chemical_list: D056804:Biofuels; D002951:Citrates; D003470:Culture Media; C514290:trisodium citrate; D000077559:Sodium Citrate; D019343:Citric Acid; D005990:Glycerol
keywords: Biodiesel-derived waste glycerol; Citric acid production; Phosphate-free detergents; Sodium citrate; Yarrowia lipolytica yeast