title: Role of protein kinase C in cytokine secretion by lung epithelial cells during infection with Paracoccidioides brasiliensis.
abstract: In this study, we investigated the role of protein kinases C (PKCs) in interleukin (IL)-6 and IL-8 secretion by human lung epithelial A549 cells during infection with the fungal pathogen Paracoccidioides brasiliensis. Rottlerin and the broad spectrum PKC inhibitor Go 6983 reduced cytokine levels in A549 cell-P. brasiliensis cultures. Next, by western blot, we verified that infection with this fungus led to phosphorylation of PKC δ (Thr(505)). By using a peptide inhibitor for PKC δ or PKC δ short interfering RNA technique, IL-6 and IL-8 levels in A549-P. brasiliensis cultures were also reduced. Together, these results indicate that P. brasiliensis promotes IL-6 and IL-8 secretion by A549 cells in a PKC δ-dependent manner.
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 6° andar, São Paulo, SP 04023-062, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Alcantara
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 6° andar, São Paulo, SP 04023-062, Brazil.
authors.forename: Paloma Korehisa
authors.initials: PK
authors.lastname: Maza
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 6° andar, São Paulo, SP 04023-062, Brazil.
authors.forename: Bianca Carla Silva Campitelli
authors.initials: BC
authors.lastname: Barros
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 6° andar, São Paulo, SP 04023-062, Brazil erika.suzuki@unifesp.br.
authors.forename: Erika
authors.initials: E
authors.lastname: Suzuki
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008962:Models, Theoretical; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000473:pathology*; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D011493:Protein Kinase C
keywords: cell signaling; cytokine; epithelial cell; fungi; innate immunity; protein kinase C