title: Characterization of microRNA in bovine in vitro culture media associated with embryo quality and development.
abstract: Dairy cattle fertility has declined over time due to factors including reduced fertilization and early embryonic loss. To counter fertility problems and better study preimplantation embryonic development, in vitro production systems have been developed. These systems largely assess embryos based on their morphology, which is not a strong indicator of developmental potential. Currently, no biomarkers can be used to noninvasively survey an embryo's potential in terms of its development and ability to establish a pregnancy. Thus, the objective of this study was to characterize and identify microRNA (miRNA) in culture media of embryos of differing developmental competence for future development as noninvasive biomarkers of embryo quality. The MiRNA sequencing of media conditioned by blastocyst and degenerate (those that failed to develop from the morula to blastocyst stage) embryos, revealed 11 differentially expressed miRNA; all were higher in concentration in degenerate conditioned media. Differential expression of mature microRNA (miR)-24, miR-191, and miR-148a was further validated using quantitative real-time PCR. Functional analysis of miR-24 revealed that addition of a mimic miRNA to culture media of morulae embryos resulted in a 27.3% decrease in development to the blastocyst stage. Furthermore, expression of miR-24 was 44.29-fold higher in blastocysts cultured with a miR-24 mimic compared with control blastocysts. Interestingly, the expression of CDKN1b, a target gene of miR-24 was repressed in embryos grown in the presence of the miRNA mimic. Mimic supplementation experiments suggest that miRNA are taken up by the embryo and that extracellular miRNA affect embryonic development. Overall, identification of a rich extracellular milieu in conditioned media sets the framework for future studies to determine the long-term predictive ability of embryo-based miRNA biomarkers on pregnancy outcome.
authors.affiliation: Department of Animal Sciences, University of Wisconsin, Madison 53706.
authors.forename: Jenna
authors.initials: J
authors.lastname: Kropp
authors.affiliation: Department of Animal Sciences, University of Wisconsin, Madison 53706. Electronic address: hkhatib@wisc.edu.
authors.forename: Hasan
authors.initials: H
authors.lastname: Khatib
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D003470:Culture Media / Q000737:chemistry*; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D005819:Genetic Markers; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009028:Morula / Q000378:metabolism; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D003470:Culture Media; D005819:Genetic Markers; D035683:MicroRNAs; D050760:Cyclin-Dependent Kinase Inhibitor p27
keywords: embryonic development; in vitro fertilization; media; microRNA; small RNA sequencing