title: Nucleolin enhances the proliferation and migration of heat-denatured human dermal fibroblasts.
abstract: Denatured dermis, a part of dermis in burned skin, has the ability to restore its normal morphology and functions after their surrounding microenvironment is improved. However, the cellular and molecular mechanisms by which the denatured dermis could improve wound healing are still unclear. This study aimed to investigate the role of nucleolin during the recovery of heat-denatured human dermal fibroblasts. Nucleolin mRNA and protein expression were significantly increased time-dependently during the recovery of heat-denatured human dermal fibroblasts (52 °C, 30 seconds). Heat-denaturation promoted a time-dependent cell proliferation, migration, chemotaxis, and scratched wound healing during the recovery of human dermal fibroblasts. These effects were prevented by knockdown of nucleolin expression with small interference RNA (siRNA), whereas overexpression of nucleolin enhanced cell proliferation, migration, and chemotaxis of human dermal fibroblasts with heat-denaturation. In addition, the expression of transforming growth factor-beta 1(TGF-β1) was significantly increased during the recovery of heat-denatured dermis and human dermal fibroblasts. TGF-β1 expression was up-regulated by nucleolin in human dermal fibroblasts. The results suggest that nucleolin expression is up-regulated, and play an important role in promoting cell proliferation, migration, and chemotaxis of human dermal fibroblasts during the recovery of heat-denatured dermis with a mechanism probably related to TGF-β1.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410008, People's Republic of China.
authors.forename: Bimei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410008, People's Republic of China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410008, People's Republic of China.
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410008, People's Republic of China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Pihong
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Dorothy M. Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Dorothy M. Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USA.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D002056:Burns / Q000503:physiopathology*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D020405:Dermis / Q000293:injuries / Q000378:metabolism / Q000503:physiopathology*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006801:Humans; D010750:Phosphoproteins / Q000494:pharmacology*; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D015854:Up-Regulation; D014945:Wound Healing; D000097796:Nucleolin
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D053773:Transforming Growth Factor beta1; D000097796:Nucleolin