title: New insight to IL-23/IL-17 axis in Iranian infected adult patients with gastritis: effects of genes polymorphisms on expression of cytokines.
abstract: OBJECTIVE
  Chronic inflammation is the hallmark of the pathogenesis of H. pylori-induced gastric cancer. IL-17A and IL-17F are inflammatory cytokines expressed by a novel subset of CD4+Th cells and play critical function in inflammation. We evaluated the relationship between IL-17A G197A, IL-17F A7488G and IL23R+2199 A/C polymorphisms with IL-6, IL-17, IL-21, IL-23 and TGF-β1 mRNAs expression in regard to H. pylori infection with chronic gastritis.
  
  
  METHODS
  Total RNA and genomic DNA were extracted from gastric biopsies of 58 H. pylori-infected patient with gastritis. Afterward, mucosal IL-6, IL-17, IL-21, IL-23 and TGF-β1 mRNAs expression and polymorphisms in IL-17A G197A, IL-17F A7488G and IL-23R +2199A/Cin gastric biopsies were determined by real-time PCR and PCR-RFLP.
  
  
  RESULTS
  Our results show that IL-17A G197A, IL-17F A7488G andIL23R +2199A/C polymorphisms have no effect on mucosal expression of IL-6, IL-17, IL-21 and TGF-β1 mRNAs expression in H. pylori-infected patients with chronic gastritis.
  
  
  CONCLUSIONS
  These results suggest that IL-17A G197A, IL-17F A7488G and IL23R +2199A/C polymorphisms no alter mucosal cytokine pattern in Iranian patients with H. pylori-associated gastritis diseases.
authors.forename: H
authors.initials: H
authors.lastname: Shirzad
authors.forename: N
authors.initials: N
authors.lastname: Bagheri
authors.forename: F
authors.initials: F
authors.lastname: Azadegan-Dehkordi
authors.forename: B
authors.initials: B
authors.lastname: Zamanzad
authors.forename: E
authors.initials: E
authors.lastname: Izadpanah
authors.forename: M
authors.initials: M
authors.lastname: Abdi
authors.forename: G
authors.initials: G
authors.lastname: Ramazani
authors.forename: M H
authors.initials: MH
authors.lastname: Sanei
authors.forename: H
authors.initials: H
authors.lastname: Ayoubian
authors.forename: A
authors.initials: A
authors.lastname: Ahmadi
authors.forename: S
authors.initials: S
authors.lastname: Jamalzehi
authors.forename: P
authors.initials: P
authors.lastname: Aslani
authors.forename: F
authors.initials: F
authors.lastname: Zandi
mesh_terms: D000328:Adult; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016480:Helicobacter pylori; D006801:Humans; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D007492:Iran; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D007378:Interleukins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1