title: Diagnostic and prognostic significance of glypican 5 and glypican 6 gene expression levels in gastric adenocarcinoma.
abstract: Gastric Cancer is one of the most common malignancies worldwide and the second most common cause of cancer-related mortality. Previous studies revealed several genetic alterations specific to gastric cancer. In this study, we aimed to investigate the diagnostic and prognostic significance of the expression levels of the glypican 5 and glypican 6 genes (GPC5andGPC6, respectively) in gastric cancer. For this purpose,GPC5andGPC6expression was quantitatively determined by quantitative polymerase chain reaction method in normal gastric mucosa and intestinal type gastric adenocarcinoma samples from 35 patients. The expression levels ofGPC5andGPC6were compared between normal and tumor tissues. Additionally, the association of the expression levels in tumor tissues with several clinicopathological parameters was evaluated. AlthoughGPC5was not expressed in any of the samples, the expression ofGPC6, which was detected in both groups, was found to be significantly higher in tumor tissues compared to that in normal samples (P=0.039). However, there was no statistically significant association betweenGPC6expression and any of the clinicopathological parameters investigated (P>0.05). Our findings suggested that an increase inGPC6expression levels may be implicated in gastric cancer development, but not in cancer progression.
authors.affiliation: Biotechnology Institute, Ankara University, Golbasi, Ankara 06830, Turkey.
authors.forename: Melike
authors.initials: M
authors.lastname: Dinccelik-Aslan
authors.affiliation: Brain Research Centre, Ankara University, Mamak, Ankara 06900, Turkey.
authors.forename: Guvem
authors.initials: G
authors.lastname: Gumus-Akay
authors.affiliation: Department of Biostatistics, Faculty of Medicine, Ankara University, Sihhiye, Ankara 06100, Turkey.
authors.forename: Atilla Halil
authors.initials: AH
authors.lastname: Elhan
authors.affiliation: Department of Surgical Oncology, Research and Training Hospital, Faculty of Medicine, Ankara University, Cebeci, Ankara 06580, Turkey.
authors.forename: Ekrem
authors.initials: E
authors.lastname: Unal
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ankara University, Sihhiye, Ankara 06100, Turkey.
authors.forename: Ajlan
authors.initials: A
authors.lastname: Tukun
keywords: expression; gastric cancer; glypican 5; glypican 6; quantitative polymerase chain reaction