title: Integration of PK/PD for dose optimization of Cefquinome against Staphylococcus aureus causing septicemia in cattle.
abstract: Cefquinome is a fourth generation cephalosporin with antimicrobial activity against gram negative and gram positive bacterial species, including Staphylococcus aureus. The aim of our study was to observe the ex-vivo activity of cefquinome against Staphylococcus aureus strains by using bovine serum from intravenously treated cattle. Cefquinome kinetics were measured by liquid chromatography and UV detection. In vitro post antibiotic effects (PAEs) and mutant prevention concentrations were determined with S. aureus strain ATCC 12598. Cefquinome exhibited time-dependent killing and produced in vitro PAEs increasing with concentration and time of exposure. A pharmacokinetic-pharmacodynamic model was established to simulate the efficacy of cefquinome for different dosage regimens. A dosage of 2 mg/kg every 12 h for 3 days was expected to reach a bactericidal activity against S. aureus in case of septicemia.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues and MAO Key Laboratory for Drug Residues Detection, Huazhong Agriculture University Wuhan, China.
authors.forename: Ijaz
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Hao
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China ; Hubei Collaborative Innovation Centre for feed Safety and Animal Nutrition, Huazhong Agricultural University Wuhan, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Huang
authors.affiliation: Laboratory of Fougères, French Agency for Food, Environmental and Occupational Safety Maisons-Alfort, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Sanders
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues and MAO Key Laboratory for Drug Residues Detection, Huazhong Agriculture University Wuhan, China ; Hubei Collaborative Innovation Centre for feed Safety and Animal Nutrition, Huazhong Agricultural University Wuhan, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues and MAO Key Laboratory for Drug Residues Detection, Huazhong Agriculture University Wuhan, China ; Hubei Collaborative Innovation Centre for feed Safety and Animal Nutrition, Huazhong Agricultural University Wuhan, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Tao
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China ; Hubei Collaborative Innovation Centre for feed Safety and Animal Nutrition, Huazhong Agricultural University Wuhan, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Xie
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China.
authors.forename: Kuang
authors.initials: K
authors.lastname: Xiuhua
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Dan
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues and MAO Key Laboratory for Drug Residues Detection, Huazhong Agriculture University Wuhan, China ; MOA Laboratory for Risk Assessment of Safety and Quality of Livestock and Poultry Products, Huazhong Agriculture University Wuhan, China ; Hubei Collaborative Innovation Centre for feed Safety and Animal Nutrition, Huazhong Agricultural University Wuhan, China.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
keywords: PK/PD; Staphylococcus aureus; cattle; cefquinome; septicemia