title: Polyclonal neural cell adhesion molecule antibody prolongs the effective duration time of botulinum toxin in decreasing muscle strength.
abstract: This study aimed to investigate if the effective duration time of botulinum toxin A (Btx-A) could be prolonged by polyclonal neural cell adhesion molecule antibody (P-NCAM-Ab). 175 male SD rats were randomly divided into three major groups: control group (n = 25), Btx-A group (n = 25), and P-NCAM-Ab groups. P-NCAM-Ab groups were composed of five sub-groups, with 25 rats each in the dose-response study. Muscle strength of rat lower limbs was determined using a survey system. The expressions of muscle-specific receptor tyrosine kinase (MuSK) and neural cell adhesion molecule (NCAM) were determined by real-time polymerase chain reactions (RT-PCR) and western blotting (WB). The muscle strength was significantly decreased by Btx-A in Btx-A/P-NCAM-Ab groups compared with normal control group. Besides, the muscle strength of P-NCAM-Ab group was significantly decreased compared with the Btx-A group. The recovery time of muscle strength in P-NCAM-Ab group was significantly longer compared with Btx-A group. RT-PCR and WB assay showed that PNCAM-Ab delayed the increase of MuSK and NCAM after Btx-A injection. P-NCAM-Ab prolongs the effective duration time of Btx-A in decreasing muscle strength, which could provide a novel enhancement in clinical application.
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China.
authors.forename: Wuchao
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China.
authors.forename: Yougui
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China. nzhiyu2002@sina.com.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Nie
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China. lingjingjin@hotmail.com.
authors.forename: Lingjing
authors.initials: L
authors.lastname: Jin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D015153:Blotting, Western; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D007273:Injections, Intramuscular; D008297:Male; D053580:Muscle Strength / Q000187:drug effects* / Q000502:physiology*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology; D019006:Neural Cell Adhesion Molecules / Q000276:immunology* / Q000378:metabolism; D009465:Neuromuscular Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism; D013995:Time
chemical_list: D000906:Antibodies; D019006:Neural Cell Adhesion Molecules; D009465:Neuromuscular Agents; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; C475048:MuSK protein, rat; D020794:Receptor Protein-Tyrosine Kinases; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; C096326:botulinum toxin type E
keywords: Botulinum toxin A; Muscle strength; Muscle-specific kinase; Neural cell adhesion molecule; Polyclonal neural cell adhesion molecule antibody