title: Coadministration of the Human Umbilical Cord Matrix-Derived Mesenchymal Cells and Aspirin Alters Postischemic Brain Injury in Rats.
abstract: BACKGROUND
  Ischemic stroke is an acute brain insult that induces dramatic changes in the neurons. Treatment of brain stroke is one of the main therapeutic targets of neuroprotective therapies. The aim of this study was to evaluate the protective potential of implanted human umbilical cord mesenchymal stem (hUCMs) cells with/without aspirin (ASA) against focal cerebral ischemia.
  
  
  METHODS
  We assessed the migration and distribution of PKH26-labeled cells after transplantation. After day 10 of transient occlusion, we evaluated the effect of ASA and hUCMs on the recovery of learning and memory in rats by Morris water maze. Afterward, animals were sacrificed, and the infarct area in the brain was evaluated using 2, 3, 5-triphenyltetrazolium chloride staining and also by hematoxylin and eosin.
  
  
  RESULTS
  The recovery of learning and memory in ischemic animals that received ASA and hUCM cells improved significantly compared with the untreated ischemic animals. Coadministration of ASA and hUCM cells did not improve the outcome at a comparable rate with ASA and hUCM cells alone. PKH26-labeled cells were detectable in the ischemic area of the brain tissue sections. 2,3,5-Triphenyltetrazolium chloride staining and histologic examinations showed that treatment with ASA and hUCM cells could significantly alter the ischemic area.
  
  
  CONCLUSIONS
  The results of the present study suggest that ASA and hUCM cells can withstand degenerative changes induced by artificial stroke in the rat. Also the learning and memory disturbance in the ASA and cell-treated animals is less pronounced than ischemic animals. Coadministration of ASA and hUCM cells did not raise the outcome higher than administration of ASA and hUCM cells alone.
authors.affiliation: Department of Anatomy, Afzalipour School of Medicine, Kerman, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shams ara
authors.affiliation: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Sheibani
authors.affiliation: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Khadije
authors.initials: K
authors.lastname: Esmaeilpour
authors.affiliation: Pharmaceutical Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Touba
authors.initials: T
authors.lastname: Eslaminejad
authors.affiliation: Department of Anatomy, Afzalipour School of Medicine, Kerman, Iran; Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: nnematollahi@kmu.ac.ir.
authors.forename: Seyed N
authors.initials: SN
authors.lastname: Nematollahi-Mahani
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D001241:Aspirin / Q000627:therapeutic use*; D001930:Brain Injuries / Q000188:drug therapy* / Q000209:etiology / Q000601:surgery*; D002545:Brain Ischemia / Q000150:complications; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D065853:Spatial Learning; D065852:Spatial Memory / Q000187:drug effects / Q000502:physiology; D016896:Treatment Outcome
chemical_list: D005343:Fibrinolytic Agents; D001241:Aspirin
keywords: Brain ischemia; TTC staining; aspirin; human umbilical cord matrix-derived mesenchymal cells; learning and memory