title: Low ozone concentrations stimulate cytoskeletal organization, mitochondrial activity and nuclear transcription.
abstract: Ozone therapy is a modestly invasive procedure based on the regeneration capabilities of low ozone concentrations and used in medicine as an alternative/adjuvant treatment for different diseases. However, the cellular mechanisms accounting for the positive effects of mild ozonization are still largely unexplored. To this aim, in the present study the effects of low ozone concentrations (1 to 20 µg O3/mL O2) on structural and functional cell features have been investigated in vitro by using morphological, morphometrical, cytochemical and immunocytochemical techniques at bright field, fluorescence and transmission electron microscopy. Cells exposed to pure O2 or air served as controls. The results demonstrated that the effects of ozoneadministration are dependent on gas concentration, and the cytoskeletal organization, mitochondrial activity and nuclear transcription may be differently affected. This suggests that, to ensure effective and permanent metabolic cell activation, ozone treatments should take into account the cytological and cytokinetic features of the different tissues.
authors.affiliation: University of Verona. manuela.costanzo@univr.it.
authors.forename: M
authors.initials: M
authors.lastname: Costanzo
authors.forename: B
authors.initials: B
authors.lastname: Cisterna
authors.forename: A
authors.initials: A
authors.lastname: Vella
authors.forename: T
authors.initials: T
authors.lastname: Cestari
authors.forename: V
authors.initials: V
authors.lastname: Covi
authors.forename: G
authors.initials: G
authors.lastname: Tabaracci
authors.forename: M
authors.initials: M
authors.lastname: Malatesta
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure*; D004305:Dose-Response Relationship, Drug; D006046:Gold; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D053758:Nanoparticles; D010126:Ozone / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010126:Ozone; D006046:Gold; D001973:Bromodeoxyuridine