title: Astrocyte-elevated gene-1 mediates insulin-like growth factor 1-induced the progression of cardiac myxoma.
abstract: Recently, astrocyte-elevated gene-1 (AEG-1) and insulin-like growth factor 1 (IGF-1) have been involved in the regulation of multiple signaling pathways in tumorigenesis. To date, the detailed mechanisms underlying IGF-1-AEG-1 pathway-induced proliferation and apoptosis in cardiac myxoma (CM) was not reported. In the present study, we used immnohistochemistry, immunoblotting, and qRT-PCR to detect the expression profile of IGF-1 and AEG-1 in 90 CM tissues, and then cultured CM cells were subjected to si-AEG-1, in vitro, and in vivo assays. Our findings showed that IGF-1 and AEG-1 were obviously upregulated in CM tissues and markedly associated with tumor size. When CM cells were treated with si-AEG-1, si-AEG-1 attenuated IGF-1-induced CM cell growth and enhanced cell apoptosis. Mechanically, we validated the expression of AEG-1, p-Erk1/2, and p-Akt increased in CM cells in response to IGF-1 treatment in a time-dependent manner. However, si-AEG-1 affected the expression of these proteins. Functionally, we found the knockdown of AEG-1-inhibited G1/S transition and tumor formation of CM cells. In conclusion, AEG-1 regulates IGF-1-induced proliferation and apoptosis via Erk1/2 and Akt signaling in CM development, which suggests IGF-1-AEG-1 signaling could be recommended to be a useful target to exert anti-tumor effects on CM.
authors.affiliation: Department of Cardiosurgery, Qilu Hospital of Shandong University, No.107 West Wenhua Road, 250012, Jinan, China.
authors.forename: Changcun
authors.initials: C
authors.lastname: Fang
authors.affiliation: Department of Cardiosurgery, Qilu Hospital of Shandong University, No.107 West Wenhua Road, 250012, Jinan, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Cardiosurgery, Qilu Hospital of Shandong University, No.107 West Wenhua Road, 250012, Jinan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qi
authors.affiliation: Department of Cardiosurgery, Qilu Hospital of Shandong University, No.107 West Wenhua Road, 250012, Jinan, China.
authors.forename: Zhengqin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Cardiosurgery, Qilu Hospital of Shandong University, No.107 West Wenhua Road, 250012, Jinan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cardiosurgery, Qilu Hospital of Shandong University, No.107 West Wenhua Road, 250012, Jinan, China. pangxinyanmd@163.com.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Pang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006338:Heart Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008875:Middle Aged; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics; D009232:Myxoma / Q000235:genetics* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D016601:RNA-Binding Proteins; D023041:Xenograft Model Antitumor Assays
chemical_list: D015815:Cell Adhesion Molecules; C583557:IGF1 protein, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D007334:Insulin-Like Growth Factor I; D051057:Proto-Oncogene Proteins c-akt; D048052:Mitogen-Activated Protein Kinase 3
keywords: AEG-1; Apoptosis; Cardiac myxoma; IGF-1; Proliferation