title: Effect of tyrosol on adhesion of Candida albicans and Candida glabrata to acrylic surfaces.
abstract: The prevention of adhesion of Candida cells to acrylic surfaces can be regarded as an alternative to prevent denture stomatitis. The use of quorum sensing molecules, such as tyrosol, could potentially interfere with the adhesion process. Therefore, the aim of this study was to assess the effect of tyrosol on adhesion of single and mixed cultures of Candida albicans and Candida glabrata to acrylic resin surfaces. Tyrosol was diluted in each yeast inoculum (10(7) cells/ml in artificial saliva) at 25, 50, 100, and 200 mM. Then, each dilution was added to wells of 24-well plates containing the acrylic specimens, and the plates were incubated at 37°C for 2 h. After, the effect of tyrosol was determined by total biomass quantification, metabolic activity of the cells and colony-forming unit counting. Chlorhexidine gluconate (CHG) was used as a positive control. Data were analyzed using analysis of variance (ANOVA) and the Holm-Sidak post hoc test (α = 0.05). The results of total biomass quantification and metabolic activity revealed that the tyrosol promoted significant reductions (ranging from 22.32 to 86.16%) on single C. albicans and mixed cultures. Moreover, tyrosol at 200 mM and CHG significantly reduced (p < 0.05) the number of adhered cells to the acrylic surface for single and mixed cultures of both species, with reductions ranging from 1.74 to 3.64-log10. In conclusion, tyrosol has an inhibitory effect on Candida adhesion to acrylic resin, and further investigations are warranted to clarify its potential against Candida infections.
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba/São Paulo, Brazil douglasrmonteiro@hotmail.com.
authors.forename: Douglas Roberto
authors.initials: DR
authors.lastname: Monteiro
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba/São Paulo, Brazil.
authors.forename: Leonardo Perina
authors.initials: LP
authors.lastname: Feresin
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba/São Paulo, Brazil.
authors.forename: Laís Salomão
authors.initials: LS
authors.lastname: Arias
authors.affiliation: Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), 13414-903 Piracicaba/São Paulo, Brazil.
authors.forename: Valentim Adelino Ricardo
authors.initials: VA
authors.lastname: Barão
authors.affiliation: Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba/São Paulo, Brazil.
authors.forename: Debora Barros
authors.initials: DB
authors.lastname: Barbosa
authors.affiliation: Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba/São Paulo, Brazil.
authors.forename: Alberto Carlos Botazzo
authors.initials: AC
authors.lastname: Delbem
mesh_terms: D000179:Acrylates; D018533:Biomass; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D041221:Candida glabrata / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D004783:Environmental Microbiology; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism; D013696:Temperature; D013995:Time
chemical_list: D000179:Acrylates; C011867:4-hydroxyphenylethanol; D010626:Phenylethyl Alcohol
keywords: Candida albicans; Candida glabrata; acrylic surface; adhesion; tyrosol