title: [Simultaneous determination of four flavonoids in Wikstroemia indica by HPLC].
abstract: The HPLC method was established to simultaneously determine the contents of myricetin, luteolin, apigenin and kaempferol in Wikstroemia indica ( L. ) C. A. Mey. The method was carried out on a Diamonsil C18 column (4. 6 mm x 250 mm, 5 µm) eluted with the mobile phases of water containing 0.15% phosphoric acid and acetonitrile in gradient mode. The UV detection wavelength was 365 nm. The flow rate was 1.0 mL · min(-1) and the column temperature was set at 30 °C. All the standard compounds showed a good linearity in the range of 0.100 8-1.008 (r = 0.999 2), 0.484 8-4.848 (r = 0.999 0) , 1. 354-13. 54 (r = 0.999 6), 0.316 8-3.168 mg · L(-1) (r = 0.999 0) for myricetin, luteolin, apigenin and kaempferol, respectively. The average recoveries of these four flavonoids were 98.5%, 100.9%, 99.7% and 98.9% with RSD 1.2%, 1.7%, 0.81% and 1.6%, respectively. In conclusion, the method is simple, rapid and accurate. It can be applied for the quality control of Wikstroemia indica.
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Sun
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Sun
authors.forename: Shan-xue
authors.initials: SX
authors.lastname: Mu
authors.forename: Lan
authors.initials: L
authors.lastname: Wei
authors.forename: Cui-ling
authors.initials: CL
authors.lastname: Xu
authors.forename: Ming-jing
authors.initials: MJ
authors.lastname: Jia
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D032365:Wikstroemia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids