title: Marine n-3 PUFA protects hearts from I/R injury  via restoration of mitochondrial function.
abstract: OBJECTIVE
  Overwhelming evidence shows that dietary supplementation with n-3 polyunsaturated fatty acids (PUFAs) elicits protective effects on patients with cardiovascular disease. However, the detailed mechanisms underlying n-3 PUFA-mediated cardioprotection are unknown, and examined in the present study.
  
  
  METHODS
  We evaluated heart performances with Langendorff perfusion apparatus. Meanwhile, whole mitochondria were purified from non-perfused hearts for functional assessment, and lipid peroxidation level was measured as well.
  
  
  RESULTS
  Compared with control groups, hearts from n-3 PUFA-supplemented rats showed improved functional recovery and reduced tissue injury following ischemia/reperfusion (I/R). Furthermore, the mitochondrial function of PUFA-treated hearts was significantly enhanced, as demonstrated by biochemical analysis of respiratory chain activity. In addition, thiobarbituric acid-reactive substance or TBARS assay revealed that lipid peroxidation product, malondialdehyde or MDA, in the mitochondria was significantly reduced by PUFA treatment.
  
  
  CONCLUSIONS
  Taken together, our data indicate that marine n-3 PUFA could improve cardiac performance after I/R injury by restoring mitochondrial respiratory activities and attenuating lipid peroxidation.
authors.affiliation: Department of Cardiology, Fu Xing Hospital, Capital Medical University, Beijing, China
authors.forename: Kang
authors.initials: K
authors.lastname: Gao
authors.forename: Liwei
authors.initials: L
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.forename: Sun
authors.initials: S
authors.lastname: Yiguang
authors.forename: Han
authors.initials: H
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Wenze
authors.initials: W
authors.lastname: Hu
authors.forename: Haifeng
authors.initials: H
authors.lastname: Liang
authors.forename: Jingguang
authors.initials: J
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology / Q000627:therapeutic use*; D005395:Fish Oils / Q000494:pharmacology / Q000627:therapeutic use*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects*; D009200:Myocardial Contraction / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D017207:Rats, Sprague-Dawley
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils