title: Magnetic Capture of a Molecular Biomarker from Synovial Fluid in a Rat Model of Knee Osteoarthritis.
abstract: Biomarker development for osteoarthritis (OA) often begins in rodent models, but can be limited by an inability to aspirate synovial fluid from a rodent stifle (similar to the human knee). To address this limitation, we have developed a magnetic nanoparticle-based technology to collect biomarkers from a rodent stifle, termed magnetic capture. Using a common OA biomarker--the c-terminus telopeptide of type II collagen (CTXII)--magnetic capture was optimized in vitro using bovine synovial fluid and then tested in a rat model of knee OA. Anti-CTXII antibodies were conjugated to the surface of superparamagnetic iron oxide-containing polymeric particles. Using these anti-CTXII particles, magnetic capture was able to estimate the level of CTXII in 25 μL aliquots of bovine synovial fluid; and under controlled conditions, this estimate was unaffected by synovial fluid viscosity. Following in vitro testing, anti-CTXII particles were tested in a rat monoiodoacetate model of knee OA. CTXII could be magnetically captured from a rodent stifle without the need to aspirate fluid and showed tenfold changes in CTXII levels from OA-affected joints relative to contralateral control joints. Combined, these data demonstrate the ability and sensitivity of magnetic capture for post-mortem analysis of OA biomarkers in the rat.
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, Biomedical Sciences Building, JG56, Gainesville, FL, 32610, USA.|Department of Medicine, University of Florida, 1600 SW Archer Rd, Gainesville, FL, 32610, USA.
authors.forename: Elena G
authors.initials: EG
authors.lastname: Yarmola
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, Biomedical Sciences Building, JG56, Gainesville, FL, 32610, USA.
authors.forename: Yash
authors.initials: Y
authors.lastname: Shah
authors.affiliation: Department of Electrical and Computer Engineering, University of Florida, 216 Larsen Hall, Gainesville, FL, 32611, USA.|Institute for Cell Engineering and Regenerative Medicine, University of Florida, 300 Weil Hall, Gainesville, FL, 32611, USA.|Nanoscience Institute for Medical and Engineering Technology (NIMET), University of Florida, 1041 Center Dr, Gainesville, FL, 32611, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Arnold
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, Biomedical Sciences Building, JG56, Gainesville, FL, 32610, USA.|Department of Materials Science and Engineering, University of Florida, 100 Rhines Hall, Gainesville, FL, 32611, USA.|Institute for Cell Engineering and Regenerative Medicine, University of Florida, 300 Weil Hall, Gainesville, FL, 32611, USA.|Nanoscience Institute for Medical and Engineering Technology (NIMET), University of Florida, 1041 Center Dr, Gainesville, FL, 32611, USA.
authors.forename: Jon
authors.initials: J
authors.lastname: Dobson
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, Biomedical Sciences Building, JG56, Gainesville, FL, 32610, USA. kyle.allen@bme.ufl.edu.|Institute for Cell Engineering and Regenerative Medicine, University of Florida, 300 Weil Hall, Gainesville, FL, 32611, USA. kyle.allen@bme.ufl.edu.|Nanoscience Institute for Medical and Engineering Technology (NIMET), University of Florida, 1041 Center Dr, Gainesville, FL, 32611, USA. kyle.allen@bme.ufl.edu.|Department of Biomedical Engineering, University of Florida, PO Box 116131, Gainesville, FL, 32611, USA. kyle.allen@bme.ufl.edu.
authors.forename: Kyle D
authors.initials: KD
authors.lastname: Allen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D024043:Collagen Type II / Q000276:immunology / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D019807:Iodoacetic Acid; D060328:Magnetic Phenomena; D008297:Male; D020370:Osteoarthritis, Knee / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013264:Stifle / Q000378:metabolism / Q000473:pathology; D013582:Synovial Fluid / Q000737:chemistry / Q000378:metabolism; D014783:Viscosity
chemical_list: D000906:Antibodies; D015415:Biomarkers; D024043:Collagen Type II; D005290:Ferric Compounds; D010446:Peptide Fragments; C000499:ferric oxide; D019807:Iodoacetic Acid
keywords: Biomarkers; Magnetic nanoparticles; Osteoarthritis; Rodent model; Synovial fluid