title: Effects of different roughage sources and feeding levels on adipogenesis of ovine adipocytes.
abstract: The objective of the present study was to conduct an adipogenic evaluation of different roughage sources and feeding levels during ruminant adipocyte differentiation in vitro. Six wether sheep were divided into a timothy hay feeding group (TFG, n = 3) and an Italian ryegrass straw feeding group (IFG, n = 3). The sheep were fed high-roughage (HR), medium roughage (MR) and low-roughage (LR) diets in a one-way layout design each over a 6-day period. Sheep serum samples collected on the last day of each dietary treatment were added to an adipogenic induction medium for differentiation of preadipocytes derived from sheep subcutaneous adipose tissue. The cytoplasmic lipid accumulations in the TFG serum-treated preadipocytes were significantly higher than those of the IFG-serum treated preadipocytes on day 12. Messenger RNA expression of CCAAT/enhancer-binding protein (C/EBP)-α, C/EBP-β, C/EBP-δ, fatty-acid-binding protein (aP2) and stearoyl-coenzyme A desaturase (SCD) were regulated by each serum treatment. This study shows that different roughage source diets and roughage-to-concentrate ratio diets can regulate adipocyte differentiation via ruminant blood composition.
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.|The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan.
authors.forename: Da-Hye
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, RDA, Chungnam, Republic of Korea.
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Toshiyoshi
authors.initials: T
authors.lastname: Ichinohe
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Sang-Houn
authors.initials: SH
authors.lastname: Song
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology; D000821:Animal Feed; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D004043:Dietary Fiber; D050356:Lipid Metabolism; D008297:Male; D012756:Sheep / Q000378:metabolism*; D040521:Subcutaneous Tissue
chemical_list: D003470:Culture Media; D004043:Dietary Fiber
keywords: adipocyte differentiation; lipid accumulation; serum treatment