title: Efficient hydrolysis of corncob residue through cellulolytic enzymes from Trichoderma strain G26 and L-lactic acid preparation with the hydrolysate.
abstract: To prepare fermentable hydrolysate from corncob residue (CCR), Trichoderma strain G26 was cultured on medium containing CCR for production of cellulolytic enzymes through solid-state fermentation (SSF), resulting in 71.3 IU/g (FPA), 136.2 IU/g (CMCase), 85.1 IU/g (β-glucosidase) and 11,344 IU/g (xylanase), respectively. Through a three-stage saccharification strategy, CCR was hydrolyzed by the enzymatic solution (6.5 FPU/ml) into fermentable hydrolysate containing 60.1g/l glucose (81.2% cellulose was converted at solid loading of 12.5%), 21.4% higher than that by the one-stage method. And then the hydrolysate was used to produce L-lactic acid by a previous screened strain Bacillus coagulans ZX25 in the submerged fermentation. 52.0 g/l L-lactic acid was obtained after fermentation for 44 h, with 86.5% glucose being converted to L-lactic acid. The results indicate that the strains and the hydrolysis strategy are promising for commercial production of L-lactic acid from CCR and other biomass.
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Xie
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Gao
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Zhewei
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Lei
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Cuiyun
authors.initials: C
authors.lastname: Ma
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Huanfei
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Fengwei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Guo
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, China. Electronic address: daiguifu@zzu.edu.cn.
authors.forename: Guifu
authors.initials: G
authors.lastname: Dai
mesh_terms: D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D019344:Lactic Acid / Q000096:biosynthesis*; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology*; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents; D014866:Waste Products; D019344:Lactic Acid; D002482:Cellulose; D002480:Cellulase
keywords: Cellulase; Corncob residue; Saccharification; Solid-state fermentation; Trichoderma longibrachiatum