title: The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling.
abstract: OBJECTIVE
  To investigate the effect of CXCL13 (C-X-C motif chemokine 13) on hepatocellular carcinoma and clarify the potential mechanisms.
  
  
  METHODS
  32 patients with hepatocellular carcinoma and 12 healthy controls were recruited for analyzing the expression of CXCL13 by RT-PCR (reverse transcription-polymerase chain reaction). ELISA (enzyme-linked immune-sorbent assay) was used to test the concentration of serum CXCL13. The interaction between CXCL13 and Wnt signaling was analyzed by western blot. In vitro PBMCs cultured with HepG2 supernatant, the levels of IL-12, IL4, IL-6, and IL-17, and four IgG subclasses were detected by ELISA.
  
  
  RESULTS
  The rate of high expression CXCL13 was 63.4% in advanced HCC patients, and the serum CXCL13 was also at a high level in stage IV HCC patients. Meanwhile CXCL13 level was positively correlated with serum ALT (Alanine Transaminase) and AST (Aspartate Aminotransferase). CXCL13 and Wnt/β-catenin signaling shared a positive feedback loop. Furthermore, CXCL13 could obviously promote the expressions of IL-12 and IL-17, and induce IgG4 secreted by B cells.
  
  
  CONCLUSIONS
  The effect of CXCL13 on promoting liver cancer is related to the activation of Wnt/β-catenin pathway and the facilitation of IL-12, IL-17 and IgG4. CXCL13 plays an important role in the progression of HCC, and it may act as a potential target for the diagnosis and treatment of HCC.
authors.affiliation: Department of Pediatric Respiratory, The First Affiliated Hospital of Jilin University, Changchun 130000, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: Huiqiao Department, South Hospital of Southern Medical University, Guangdong 510000, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Kang
authors.affiliation: Department of Hand Surgery, The First Affiliated Hospital of Jilin University, Changchun 130000, China.
authors.forename: Xiguang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Pediatric Digestive Department, The First Affiliated Hospital of Jilin University, Changchun 130000, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiratory, The Third Affiliated Hospital of Southern Medical University, Guangdong 510000, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Hepatobiliary and Pancreatic Diseases, The First Affiliated Hospital of Jilin University, Changchun 130000, China.
authors.forename: Pujun
authors.initials: P
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000378:metabolism*; D016764:Cell Polarity / Q000187:drug effects; D054382:Chemokine CXCL13 / Q000097:blood / Q000494:pharmacology*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D008113:Liver Neoplasms / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D007074:Immunoglobulin G; D020381:Interleukin-17; D051176:beta Catenin; D018664:Interleukin-12