title: Effects of Deep Electroacupuncture Stimulation at "Huantiao" (GB 30) on Expression of Apoptosis-Related Factors in Rats with Acute Sciatic Nerve Injury.
abstract: SD rats were randomly divided into normal control, model, deep EA, and shallow EA groups. The model was established by mechanical clamping of the sciatic nerve stem. For deep and shallow EA, the needles were inserted into "Huantiao" (GB 30) by about 16 mm and 7 mm, respectively, once daily for 14 days. The results showed that, compared with the normal control group, the nerve-muscle excitability of rat's hip muscle decreased and the nerve conduction velocity of sciatic nerve slowed down in the model group; meanwhile, the number of apoptotic cells and the expression level of Bax protein in the injured nerve increased significantly, and the expression level of Bcl-2 protein and the ratio of Bcl-2/Bax decreased considerably. Compared with the model group, the indices mentioned above were reversed in the two treatment groups, and the changes in the deep EA group were more significant than those in the shallow EA group. These results indicate that EA stimulation at GB 30 can improve the function of injured sciatic nerve, which is closely associated with its effects in upregulating the expression of apoptosis inhibitive factor Bcl-2 and downregulating apoptosis promotive factor Bax. Deep EA is relatively better.
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China ; Liaoning Health Vocational and Technical College, Shenyang 110101, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Dai
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Han
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
authors.forename: Tieming
authors.initials: T
authors.lastname: Ma
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Ren
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
authors.forename: Zenghua
authors.initials: Z
authors.lastname: Bai
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Benxi Campus, Benxi 117000, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li