title: Gene Variant and Level of IL-1β in Ischemic Stroke Patients With and Without Type 2 Diabetes Mellitus.
abstract: Evidence is emerging that inflammation plays a key role in the pathophysiology of ischemic stroke (IS). The aim of this study was to explore, for the first time, the relationship between IL-1β -31 T/C polymorphism and the risk of ischemic stroke (IS) among patients with type 2 diabetes mellitus (T2DM). One hundred ninety-six patients with IS (117 diabetics and 79 nondiabetics) and 192 controls were recruited to enroll in this study. IL-1β genotyping was performed by PCR-RFLP technique. After adjusting for sex, age, smoking, obesity, dyslipidemia, and hypertension, there was no significant difference in the distribution of IL-1β -31 T/C genotypes and allele frequencies between IS patients with or without type 2 diabetes mellitus and control group (p > 0.05). Moreover, a significant positive correlation between serum IL-1β level and glucose (p1 = 0.044) was showed. In addition, serum levels of IL-1β were found to be higher among TT genotype carriers than TC and CC genotype carriers in ischemic stroke patients with or without T2DM but these differences were not significant. These results indicate that IL-1β gene polymorphism might not be a risk factor in the development of ischemic stroke in Tunisian population.
authors.affiliation: Research Unit: UR 12ES09 Dyslipidemia and Atherogenesis, Faculty of Medicine, Monastir, 5019, Tunisia. khouloudchehaibi5@gmail.com.
authors.forename: Khouloud
authors.initials: K
authors.lastname: Chehaibi
authors.affiliation: ResearchUnit: UR 07/06, Faculty of Pharmacy, Monastir, 5019, Tunisia.
authors.forename: Mohamed Yahia
authors.initials: MY
authors.lastname: Hrira
authors.affiliation: Research Unit: UR 12ES09 Dyslipidemia and Atherogenesis, Faculty of Medicine, Monastir, 5019, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Trabelsi
authors.affiliation: Institut d'Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain.|Departament de Bioquímica i Biología Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Escolà-Gil
authors.affiliation: Research Unit: UR 12ES09 Dyslipidemia and Atherogenesis, Faculty of Medicine, Monastir, 5019, Tunisia.
authors.forename: Mohamed Naceur
authors.initials: MN
authors.lastname: Slimane
mesh_terms: D000368:Aged; D000483:Alleles; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D002545:Brain Ischemia / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D015897:Comorbidity; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007249:Inflammation; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking / Q000453:epidemiology; D014416:Tunisia / Q000453:epidemiology
chemical_list: D001786:Blood Glucose; D053583:Interleukin-1beta; D008055:Lipids; D008074:Lipoproteins
keywords: IL-1β polymorphism; Inflammation; Ischemic stroke; Type 2 diabetes mellitus