title: 2,4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp.
abstract: The volatile organic compound 2,4-di-tert-butyl phenol (2,4 DTBP) was purified from the cell free supernatant of a newly isolated Lactococcus sp. by solvent extraction and chromatographic techniques. Molecular characterization of the compound by ESI-MS, (1)H NMR and FTIR analysis revealed the structure, C14H22O. Fungicidal activity was demonstrated against Aspergillus niger, Fusarium oxysporum and Penicillium chrysogenum by disc diffusion assay. Among the cell lines tested for cytotoxicity of this compound (normal cell line H9c2 and cancer cell lines HeLa and MCF-7), a remarkable cytotoxicity against HeLa cells with an IC50 value of 10 μg/mL was shown. A biocontrol experiment with 2,4 DTBP supplemented fraction prevented growth of the abovementioned fungi on wheat grains. The study further strengthens the case for development of biopreservatives and dietary antioxidants from lactic acid bacteria for food applications.
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
authors.forename: Kontham Kulangara
authors.initials: KK
authors.lastname: Varsha
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
authors.forename: Leena
authors.initials: L
authors.lastname: Devendra
authors.affiliation: Agroprocessing Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Shilpa
authors.affiliation: Agroprocessing Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
authors.forename: Sulochana
authors.initials: S
authors.lastname: Priya
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India. Electronic address: madhavan85@hotmail.com.
authors.forename: Kesavan Madhavan
authors.initials: KM
authors.lastname: Nampoothiri
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D045744:Cell Line, Tumor; D002470:Cell Survival; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D017045:Lactococcus / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D010636:Phenols; C056559:2,4-di-tert-butylphenol
keywords: 2,4-Di-tert-butyl phenol; Antifungal; Antioxidant; Biopreservation; Cytotoxicity; Lactic acid bacteria