title: Multilayer Hydrophilic Poly(phenol-formaldehyde resin)-Coated Magnetic Graphene for Boronic Acid Immobilization as a Novel Matrix for Glycoproteome Analysis.
abstract: Capturing glycopeptides selectively and efficiently from mixed biological samples has always been critical for comprehensive and in-depth glycoproteomics analysis, but the lack of materials with superior capture capacity and high specificity still makes it a challenge. In this work, we introduce a way first to synthesize a novel boronic-acid-functionalized magnetic graphene@phenolic-formaldehyde resin multilayer composites via a facile process. The as-prepared composites gathered excellent characters of large specific surface area and strong magnetic responsiveness of magnetic graphene, biocompatibility of resin, and enhanced affinity properties of boronic acid. Furthermore, the functional graphene composites were shown to have low detection limit (1 fmol) and good selectivity, even when the background nonglycopeptides has a concentration 100 fold higher. Additionally, enrichment efficiency of the composites was still retained after being used repeatedly (at least three times). Better yet, the practical applicability of this approach was evaluated by the enrichment of human serum with a low sample volume of 1 μL. All the results have illustrated that the magG@PF@APB has a great potential in glycoproteome analysis of complex biological samples.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D001897:Boronic Acids / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D005557:Formaldehyde / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010636:Phenols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D066330:Printing, Three-Dimensional; D020543:Proteome / Q000032:analysis*
chemical_list: D001897:Boronic Acids; D020099:Coated Materials, Biocompatible; D006023:Glycoproteins; D058185:Magnetite Nanoparticles; D010636:Phenols; D011108:Polymers; D020543:Proteome; D005557:Formaldehyde; D006108:Graphite; C011529:phenol-formaldehyde resin
keywords: MALDI-TOF mass spectrometry; boronic-acid-functionalized magnetic graphene; enrichment; glycoproteome; phenolic-formaldehyde resin