title: [Exploring the stem cell surface markers expressed in human dental pulp stem cells].
abstract: OBJECTIVE
  To explore the stem cell surface markers expressed in human dental pulp stem cells which were selected and isolated by magnetic beads.
  
  
  METHODS
  Human dental pulp cells (hDPCs) were separated and cultured from dental pulp of healthy third molars for orthodontic purpose. HDPSCs were isolated from cultured hDPCs by magnetic-activated cell sorting's (MACS) indirect magnetic cell labeling and positive selection strategy with antibody STRO-1 in the 2nd generation. Then the stem cell surface markers (CD73, CD90, CD105, CD166 and STRO-1) were respectively detected in 3, 4, 5, 6, 7 and 8 generation of dental pulp stem cells. HDPSCs were induced to differentiation by adipogenic medium and osteogenic medium in the 3rd generation. Adipogenic differentiation was assessed by oil red O staining in day 21, and osteogenic differentiation was assessed by alizarin red staining in day 21.
  
  
  RESULTS
  HDPSCs could differentiate into adipocyte and osteoblasts. Oil red O staining and alizarin red staining were positively expressed after induction of HDPSCs. STRO-1's expression was decreased with the increase of generation. The expressions of CD73, CD90, CD105 and CD166 were relatively stable.
  
  
  CONCLUSIONS
  The expression of STRO-1 is declined with the increase of generation, and the expressions of CD73, CD90, CD105 and CD166 are relatively stable with the changes of generation. Supported by National Natural Science Foundation of China (81070826/81371143) and Shanghai Rising-Star Program (12QH1401400).
authors.affiliation: epartment of Endodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011; China. E-mail:1127239423@sjtu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Wen-xin
authors.initials: WX
authors.lastname: Jiang
authors.forename: Cheng-guang
authors.initials: CG
authors.lastname: Niu
authors.forename: Zhi-yan
authors.initials: ZY
authors.lastname: He
authors.forename: Cai-lian
authors.initials: CL
authors.lastname: Zhu
authors.forename: Zheng-wei
authors.initials: ZW
authors.lastname: Huang
mesh_terms: D015415:Biomarkers; D002454:Cell Differentiation; D002469:Cell Separation; D003782:Dental Pulp; D006801:Humans; D010006:Osteoblasts; D010012:Osteogenesis; D013234:Stem Cells
chemical_list: D015415:Biomarkers