title: Development and preclinical evaluation of a new galactomannan-based dressing with antioxidant properties for wound healing.
abstract: We describe a novel wound dressing (HR006) with two components: a lyophilized matrix of the galactomannan from locust bean gum (LBG) and an antioxidant hydration solution (AHsol) containing curcumin and N-acetyl-L-cysteine (NAC). Physico-structural analyses of the LBG matrix revealed homogeneous interconnected pores with high absorbing capacity showing excellent properties for moist wound care (MWC). In an in vitro oxidative stress fibroblast injury model, the AHsol showed relevant protective effects reducing intracellular reactive oxygen species (ROS) production, rescuing cell viability, and regulating expression of inflammation-related genes (COX-2, TNF-α, IL-1α, IL-1β). The new dressing showed good biocompatibility profile as demonstrated by cytotoxicity, hemocompatibility, and skin irritation tests. Moreover, in an in vivo skin wound model in pigs, this dressing enhanced the production of healthy and organized granulation tissue and re-epithelization. In summary, HR006 exhibits significant antioxidant activity, good biocompatibility, and excellent repair capabilities improving tissue remodeling and the healing of wounds.
authors.affiliation: Histocell, Bizkaia Science and Technology Park, Derio, Bizkaia, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Castro
authors.affiliation: Faculty of Medicine and Dentistry, University of the Basque Country, UPV/EHU, Spain.
authors.forename: Teodoro
authors.initials: T
authors.lastname: Palomares
authors.affiliation: Histocell, Bizkaia Science and Technology Park, Derio, Bizkaia, Spain.
authors.forename: Iker
authors.initials: I
authors.lastname: Azcoitia
authors.affiliation: ArtinVet, Bizkaia Science and Technology Park, Derio, Bizkaia, Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Bastida
authors.affiliation: Histocell, Bizkaia Science and Technology Park, Derio, Bizkaia, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: del Olmo
authors.affiliation: Hospital de San Pedro, Servicio Riojano de Salud, La Rioja, Spain.
authors.forename: Javier J
authors.initials: JJ
authors.lastname: Soldevilla
authors.affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country, UPV/EHU, Spain. ana.alonsovarona@ehu.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Alonso-Varona
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000627:therapeutic use*; D001458:Bandages / Q000009:adverse effects; D003474:Curcumin / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects; D005685:Galactans; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics; D007509:Irritants; D008351:Mannans / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D008422:Materials Testing; D018384:Oxidative Stress / Q000187:drug effects; D053149:Plant Gums; D017382:Reactive Oxygen Species / Q000378:metabolism; D034421:Sus scrofa; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D005685:Galactans; D007509:Irritants; D008351:Mannans; D053149:Plant Gums; D017382:Reactive Oxygen Species; C012990:galactomannan; D003474:Curcumin; C017471:locust bean gum; D000111:Acetylcysteine; D005690:Galactose