title: Intracellular localizations of the Dead End protein in Xenopus primordial germ cells.
abstract: We investigated the intracellular localization of Xenopus Dead end protein (Dnd1) in primordial germ cells during early development by expressing the tagged protein in transgenic Xenopus embryos, with the germ plasm visualized. Dnd1 initially localized to the germ plasm in the cortex, moved to the perinuclear region together with the germ plasm after the midblastula transition, and then entered the nucleus. Using Dnd1 deletion mutants, we identified two distinct but overlapping regions of Dnd1 that were responsible for localization to either the germ plasm or nucleus. These Dnd1 regions appeared to function in primordial germ cell- and stage-specific manners.
authors.affiliation: Department of Life Science, University of Hyogo, Kamigori, Akou-gun, Hyogo, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Taguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Orii
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D036703:Blastula / Q000196:embryology / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D005854:Germ Cells / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008870:Microtubules / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis
chemical_list: C000624414:Dnd1 protein, Xenopus; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D029867:Xenopus Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins