title: Amyloid-β in the Cerebrospinal Fluid of APP Transgenic Mice Does not Show Prion-like Properties.
abstract: Early diagnosis of Alzheimer`s disease (AD) is currently difficult and involves a complex approach including clinical assessment, neuroimaging, and measurement of amyloid-β (Aβ) and tau levels in cerebrospinal fluid (CSF). A better mechanistic understanding is needed to develop more accurate and even presymptomatic diagnostic tools. It has been shown that Aβ derived from amyloid-containing brain tissue has prion-like properties: it induces misfolding and aggregation of Aβ when injected into human amyloid precursor protein (APP) transgenic mice. In contrast, Aβ in the CSF has been less studied, and it is not clear whether it also exhibits prion-like characteristics, which might provide a sensitive diagnostic tool. Therefore, we collected CSF from APP transgenic mice carrying the Swedish mutation (APP23 mice), and injected it intracerebrally into young mice from the same transgenic line. We found that CSF derived Aβ did not induce increased β-amyloidosis, even after long incubation periods and additional concentration. This suggests that Aβ present in the CSF does not have the same prion-like properties as the Aβ species in the brain.
authors.forename: Zhiva
authors.initials: Z
authors.lastname: Skachokova
authors.forename: Frederik
authors.initials: F
authors.lastname: Sprenger
authors.forename: Karin
authors.initials: K
authors.lastname: Breu
authors.forename: Dorothee
authors.initials: D
authors.lastname: Abramowski
authors.forename: Florence
authors.initials: F
authors.lastname: Clavaguera
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hench
authors.forename: Matthias
authors.initials: M
authors.lastname: Staufenbiel
authors.forename: Markus
authors.initials: M
authors.lastname: Tolnay
authors.affiliation: Institute of Pathology, University Hospital Basel, Basel, Switzerland. david.winkler@usb.ch.
authors.forename: David T
authors.initials: DT
authors.lastname: Winkler
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000008:administration & dosage* / Q000134:cerebrospinal fluid*; D000686:Amyloidosis / Q000378:metabolism; D000818:Animals; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011328:Prions / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D011328:Prions