title: Possible Association of IFN-γ Gene -316A/G SNP with Humoral Immune Response to Killed H5N1 HPAI Vaccine in a Red Junglefowl Population.
abstract: To understand the role of interferon (IFN)-γ gene (IFNG) single-nucleotide polymorphisms (SNPs) in the resistance to H5N1 highly pathogenic avian influenza (HPAI), we determined the SNPs, the log2-transformed specific antibody titers, and ex vivo recall antigen-specific IFN-γ production by peripheral blood mononuclear cells (PBMCs) in 62 red junglefowls (Gallus gallus) immunized twice with inactivated H5N1 HPAI vaccine. Consequently, 52 SNPs were detected in the amplified 1137-bp length covering the promoter region and all exons, with +2133A/T SNP in the coding DNA sequence that caused a missense mutation and was identified in G. gallus for the first time. For -316A/G SNP genotypes, the chi-squared test showed that the bird sample was in the Hardy-Weinberg equilibrium (P=0.369>0.05), and the least squares analysis revealed an increasing tendency in the antibody titers with G to A substitution so that a significant difference occurred between the AA genotype (8.031±0.247) and the GG genotype (6.571±0.528) (P=0.015<0.05, 95% confidence interval [CI]: 0.0004-0.0866), as basically consistent with the antigen-induced IFN-γ protein expression, which indicated possible association of the -316A/G SNP with a secondary humoral immune response to the HPAI vaccine in the bird population. These findings may help to improve genetic resistance using cross-breeding and enhance HPAI vaccine-induced immunity in chicken production.
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Sun
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Ma
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Qiong-Fen
authors.initials: QF
authors.lastname: Lu
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Hu
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Zong-Hui
authors.initials: ZH
authors.lastname: Jian
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Lab of Veterinary Public Health of Yunnan Higher Education Institutes, Division of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University , Kunming, People's Republic of China .
authors.forename: Pei-Fu
authors.initials: PF
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000821:virology; D056724:Immunity, Humoral / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology* / Q000821:virology; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D017422:Sequence Analysis, DNA; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D007371:Interferon-gamma