title: Celecoxib and Ibuprofen Restore the ATP  Content and the Gluconeogenesis Activity  in the Liver of Walker-256 Tumor-Bearing  Rats.
abstract: OBJECTIVE
  The main purpose of this study was to investigate the effects of celecoxib and ibuprofen, both non-steroidal anti-inflammatory drugs (NSAIDs), on the decreased gluconeogenesis observed in liver of Walker-256 tumor-bearing rats.
  
  
  METHODS
  Celecoxib and ibuprofen (both at 25 mg/Kg) were orally administered for 12 days, beginning on the same day when the rats were inoculated with Walker-256 tumor cells.
  
  
  RESULTS
  Celecoxib and ibuprofen treatment reversed the reduced production of glucose, pyruvate, lactate and urea from alanine as well as the reduced production of glucose from pyruvate and lactate in perfused liver from tumor-bearing rats. Besides, celecoxib and ibuprofen treatment restored the decreased ATP content, increased triacylglycerol levels and reduced mRNA expression of carnitine palmitoyl transferase 1 (CPT1), while ibuprofen treatment restored the reduced mRNA expression of peroxisome proliferator-activated receptor alpha (PPARα) in the liver of tumor-bearing rats. Both treatments tended to decrease TNFα, IL6 and IL10 in the liver of tumor-bearing rats. Finally, the treatment with celecoxib, but not with ibuprofen, reduced the growth of Walker-256 tumor.
  
  
  CONCLUSIONS
  Celecoxib and ibuprofen restored the decreased gluconeogenesis in the liver of Walker-256 tumor-bearing rats. These effects did not involve changes in tumor growth and probably occurred by anti-inflammatory properties of these NSAIDs, which increased expression of genes associated with fatty acid oxidation (PPARα and CPT1) and consequently the ATP production, normalizing the energy status in the liver of tumor-bearing rats.
authors.forename: Camila Oliveira
authors.initials: CO
authors.lastname: de Souza
authors.forename: Mirian Ayumi
authors.initials: MA
authors.lastname: Kurauti
authors.forename: Flaviane de Fatima
authors.initials: Fde F
authors.lastname: Silva
authors.forename: Hely
authors.initials: H
authors.lastname: de Morais
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
authors.forename: Sandro Massao
authors.initials: SM
authors.lastname: Hirabara
authors.forename: José Cesar
authors.initials: JC
authors.lastname: Rosa Neto
authors.forename: Helenir Medri
authors.initials: HM
authors.lastname: de Souza
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D000068579:Celecoxib / Q000494:pharmacology / Q000627:therapeutic use*; D005943:Gluconeogenesis / Q000187:drug effects*; D007052:Ibuprofen / Q000494:pharmacology / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009369:Neoplasms / Q000150:complications / Q000188:drug therapy / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000255:Adenosine Triphosphate; D000068579:Celecoxib; D007052:Ibuprofen