title: Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater.
abstract: Acinetobacter junii YB was found to exhibit efficient heterotrophic nitrification and aerobic denitrification ability, with the maximum ammonium, nitrite and nitrate removal rate of 8.82, 8.45 and 7.98 mg/L/h, respectively. Meanwhile, ammonium was found to be removed preferentially in the process of simultaneous nitrification and denitrification in mixed N-sources. The successful PCR amplification of hao, napA and nirS genes further provided additional evidence of heterotrophic nitrification and aerobic denitrification by strain YB. In addition, orthogonal test showed that dissolved oxygen was the most important determinant of nitrite removal, and the optimal conditions were C/N 15, pH 7.0, 37 °C and 200 rpm. Furthermore, stable nitrogen and organics removal were achieved by one-time dosing of enriched bacteria in a sequencing batch reactor. The inoculation of strain YB significantly improved the denitrification efficiency with minimal accumulation of nitrified products, which demonstrated high potential of the isolate for future practical applications.
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: ryx@xauat.edu.cn.
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Ren
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Liang
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Si-Qi
authors.initials: SQ
authors.lastname: Zhao
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Wang
authors.affiliation: Shaanxi Architectural Design & Research Institute Co., Ltd., Xi'an 710003, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Xia
mesh_terms: D000150:Acinetobacter / Q000378:metabolism / Q000502:physiology*; D000332:Aerobiosis / Q000502:physiology; D064751:Ammonium Compounds / Q000378:metabolism; D058440:Denitrification / Q000502:physiology*; D052836:Heterotrophic Processes / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism; D058465:Nitrification / Q000502:physiology; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013696:Temperature; D062065:Wastewater / Q000382:microbiology*
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D010100:Oxygen; D062065:Wastewater
keywords: Acinetobacter junii YB; Heterotrophic nitrification–aerobic denitrification; Nitrite removal; Nitrogen removal mechanism