title: Multiscale vision model for event detection and reconstruction in two-photon imaging data.
abstract: Reliable detection of calcium waves in multiphoton imaging data is challenging because of the low signal-to-noise ratio and because of the unpredictability of the time and location of these spontaneous events. This paper describes our approach to calcium wave detection and reconstruction based on a modified multiscale vision model, an object detection framework based on the thresholding of wavelet coefficients and hierarchical trees of significant coefficients followed by nonlinear iterative partial object reconstruction, for the analysis of two-photon calcium imaging data. The framework is discussed in the context of detection and reconstruction of intercellular glial calcium waves. We extend the framework by a different decomposition algorithm and iterative reconstruction of the detected objects. Comparison with several popular state-of-the-art image denoising methods shows that performance of the multiscale vision model is similar in the denoising, but provides a better segmenation of the image into meaningful objects, whereas other methods need to be combined with dedicated thresholding and segmentation utilities.
authors.affiliation: Moscow State University , Faculty of Biology, Moscow 119234, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Brazhe
authors.affiliation: Copenhagen University , Institute for Neuroscience and Pharmacology, Copenhagen 2200, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Mathiesen
authors.affiliation: Copenhagen University , Institute for Neuroscience and Pharmacology, Copenhagen 2200, Denmark.
authors.forename: Barbara
authors.initials: B
authors.lastname: Lind
authors.affiliation: Moscow State University , Faculty of Biology, Moscow 119234, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Rubin
authors.affiliation: Copenhagen University , Institute for Neuroscience and Pharmacology, Copenhagen 2200, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Lauritzen
keywords: astrocyte; calcium imaging; calcium wave; multiscale vision model; wavelet transforms