title: In vitro antitumor immune response induced by dendritic cells transduced with human livin α recombinant adenovirus.
abstract: Transduction with recombinant, replication-defective adenoviral (rAd) vectors encoding a transgene is an efficient method for gene transfer into human dendritic cells (DCs). Livin is a good candidate for cancer immunotherapy since it is overexpressed in most common human cancers, poorly expressed in most normal adult tissues. Two splicing variants of livin, designated livin α and livin β, have been identified. In this study, we used human livin α recombinant adenovirus (rAd-hlivin α) to transduced DCs. We found that DCs transduced with rAd-hlivin α (rAd-hlivin α DCs) could effectively induce human livin α specific cytotoxic T lymphocytes (CTL) in vitro against various tumor cell lines.
authors.affiliation: Department of Respiratory Disease, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: junpingxie@sina.com.
authors.forename: Junping
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Respiratory Disease, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China; The Key Laboratory of Molecular Medicine of Jiangxi Province, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Respiratory Disease, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China; The Key Laboratory of Molecular Medicine of Jiangxi Province, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Respiratory Disease, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Respiratory Disease, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Duan
authors.affiliation: Department of Respiratory Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Tao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000256:Adenoviridae; D019496:Cancer Vaccines / Q000276:immunology; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D061986:MCF-7 Cells; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D020033:Protein Isoforms / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D019496:Cancer Vaccines; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D007371:Interferon-gamma
keywords: Dendritic cell; Gene therapy; Livin α; Recombinant adenoviral vector; Tumor cell