title: Fluorescence-guided surgery of human prostate cancer experimental bone metastasis in nude mice using anti-CEA DyLight 650 for tumor illumination.
abstract: The present report demonstrates efficacy of fluorescence-guided surgery (FGS) to resect and prevent recurrence of experimental skeletal metastasis in a nude-mouse model of human prostate cancer. Green fluorescent protein (GFP)-expressing PC-3 human prostate cancer cells were injected into the intramedullary cavity of the tibia in 25 nude mice. One week after implantation, monoclonal antibodies, specific for carcinoembryonic antigen (CEA), labeled with DyLight 650, were injected into the tail vein of 13 mice. Thirteen mice underwent FGS and 12 mice underwent bright-light surgery (BLS). Weekly GFP fluorescence imaging of the mice was performed to observe tumor recurrence. The extent of residual tumor after BLS was 13-fold greater than after FGS (p < 0.001). Time-course imaging visualized rapid growth of the residual tumor in the BLS group, whereas the FGS group showed only slight tumor growth and significantly improved disease-free survival of the treated mice. Our study demonstrated that FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis. The present results suggest that FGS will be effective for resection of skeletal metastases in selected patients with prostate cancer.
authors.affiliation: AntiCancer, Inc., San Diego, California.|Department of Surgery, University of California, San Diego, San Diego, California.|Department of Orthopedic Surgery, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.affiliation: Department of Surgery, University of California, San Diego, San Diego, California.
authors.forename: Nzola
authors.initials: N
authors.lastname: De Magalhães
authors.affiliation: AntiCancer, Inc., San Diego, California.|Department of Surgery, University of California, San Diego, San Diego, California.
authors.forename: Makoto
authors.initials: M
authors.lastname: Toneri
authors.affiliation: AntiCancer, Inc., San Diego, California.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: AntiCancer, Inc., San Diego, California.
authors.forename: Wenluo
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Surgery, University of California, San Diego, San Diego, California.
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: Department of Orthopedic Surgery, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.affiliation: AntiCancer, Inc., San Diego, California.|Department of Surgery, University of California, San Diego, San Diego, California.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000556:secondary / Q000601:surgery*; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D008819:Mice, Nude; D009364:Neoplasm Recurrence, Local / Q000453:epidemiology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000601:surgery; D061848:Optical Imaging / Q000379:methods*; D011471:Prostatic Neoplasms / Q000473:pathology*; D011897:Random Allocation
keywords: CEA; GFP; bone metastasis; fluorescence-guided surgery; prostate cancer