title: Evaluation environment for digital and analog pathology: a platform for validation studies.
abstract: We present a platform for designing and executing studies that compare pathologists interpreting histopathology of whole slide images (WSIs) on a computer display to pathologists interpreting glass slides on an optical microscope. eeDAP is an evaluation environment for digital and analog pathology. The key element in eeDAP is the registration of the WSI to the glass slide. Registration is accomplished through computer control of the microscope stage and a camera mounted on the microscope that acquires real-time images of the microscope field of view (FOV). Registration allows for the evaluation of the same regions of interest (ROIs) in both domains. This can reduce or eliminate disagreements that arise from pathologists interpreting different areas and focuses on the comparison of image quality. We reduced the pathologist interpretation area from an entire glass slide (10 to [Formula: see text]) to small ROIs ([Formula: see text]). We also made possible the evaluation of individual cells. We summarize eeDAP's software and hardware and provide calculations and corresponding images of the microscope FOV and the ROIs extracted from the WSIs. The eeDAP software can be downloaded from the Google code website (project: eeDAP) as a MATLAB source or as a precompiled stand-alone license-free application.
authors.affiliation: FDA/CDRH/OSEL , Division of Imaging, Diagnostics, and Software Reliability, 10903 New Hampshire Avenue, Building 62, Room 3124, Silver Spring, Maryland 20993-0002, United States.
authors.forename: Brandon D
authors.initials: BD
authors.lastname: Gallas
authors.affiliation: FDA/CDRH/OSEL , Division of Imaging, Diagnostics, and Software Reliability, 10903 New Hampshire Avenue, Building 62, Room 3124, Silver Spring, Maryland 20993-0002, United States.
authors.forename: Marios A
authors.initials: MA
authors.lastname: Gavrielides
authors.affiliation: National Cancer Institute , National Institutes of Health, Center for Cancer Research, Laboratory of Pathology, 10 Center Drive, MSC 1500, Bethesda, Maryland 20892, United States.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Conway
authors.affiliation: FDA/CDRH/OSEL , Division of Imaging, Diagnostics, and Software Reliability, 10903 New Hampshire Avenue, Building 62, Room 3124, Silver Spring, Maryland 20993-0002, United States.
authors.forename: Adam
authors.initials: A
authors.lastname: Ivansky
authors.affiliation: FDA/CDRH/OSEL , Division of Imaging, Diagnostics, and Software Reliability, 10903 New Hampshire Avenue, Building 62, Room 3124, Silver Spring, Maryland 20993-0002, United States.
authors.forename: Tyler C
authors.initials: TC
authors.lastname: Keay
authors.affiliation: FDA/CDRH/OSEL , Division of Imaging, Diagnostics, and Software Reliability, 10903 New Hampshire Avenue, Building 62, Room 3124, Silver Spring, Maryland 20993-0002, United States.
authors.forename: Wei-Chung
authors.initials: WC
authors.lastname: Cheng
authors.affiliation: National Cancer Institute , National Institutes of Health, Center for Cancer Research, Laboratory of Pathology, 10 Center Drive, MSC 1500, Bethesda, Maryland 20892, United States.
authors.forename: Jason
authors.initials: J
authors.lastname: Hipp
authors.affiliation: National Cancer Institute , National Institutes of Health, Center for Cancer Research, Laboratory of Pathology, 10 Center Drive, MSC 1500, Bethesda, Maryland 20892, United States.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
keywords: digital pathology; microscopy; reader studies; technology evaluation; validation; whole slide imaging