title: Severe Acquired Idiopathic Thrombocytopenia in a Female Cynomolgus Macaque (Macaca fascicularis).
abstract: A 4-y-old female cynomolgus macaque presented for veterinary evaluation prior to placement in a preclinical study showed markedly low platelet counts that continued to decrease over time. Physical examination over the next several days showed areas of pale red discoloration in forelimbs, anterior thorax, and inguinal area and multifocal pinpoint areas of erythema or scabs. An area of dark red discoloration approximately 2 cm in diameter on the dorsal surface of the tongue was discovered on day 9. The macaque was euthanized, and histopathologic evaluation showed multifocal, ulcerative or erosive, hemorrhagic, lymphohistiocytic and neutrophilic glossitis and tonsillitis. The lesions on the tongue were associated with opportunistic fungi consistent with Candida albicans. The bone marrow showed megakaryocytic hyperplasia. There was no evidence of increased consumption of platelets, sequestration of platelets, or bone marrow suppression. The monkey was serologically negative for simian retrovirus, SIV, and simian T-lymphotropic virus. In light of cases reported in humans, immune-mediated destruction of platelets due to autoantibodies secondary to Candida albicans infection was considered. However, we were unable to detect antiplatelet antibodies on the platelet surface or in serum to support this etiology; therefore idiopathic thrombocytopenia was diagnosed. To our knowledge, this case represents the second reported observation of acquired thrombocytopenia in a nonhuman primate and the first reported observation in a cynomolgus macaque.
authors.affiliation: Department of Pathology, Bristol-Myers Squibb Company, New Brunswick, New Jersey, USA. cecilia.parrula@bms.com.
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Parrula
authors.affiliation: Department of Pathology, Bristol-Myers Squibb Company, New Brunswick, New Jersey, USA.
authors.forename: Jagannatha
authors.initials: J
authors.lastname: Mysore
authors.affiliation: Department of Veterinary Sciences, Bristol-Myers Squibb Company, New Brunswick, New Jersey, USA.
authors.forename: Holly
authors.initials: H
authors.lastname: Burr
authors.affiliation: Department of Immunotoxicology, Drug Safety Evaluation, Research, and Development, Bristol-Myers Squibb Company, New Brunswick, New Jersey, USA.
authors.forename: Wendy
authors.initials: W
authors.lastname: Freebern
authors.affiliation: Department of Pathology, Bristol-Myers Squibb Company, New Brunswick, New Jersey, USA.
authors.forename: Natasha
authors.initials: N
authors.lastname: Neef
mesh_terms: D000818:Animals; D005260:Female; D008252:Macaca fascicularis; D012720:Severity of Illness Index; D013921:Thrombocytopenia / Q000175:diagnosis* / Q000473:pathology