title: Human turbinate mesenchymal stromal cell sheets with bellows graft for rapid tracheal epithelial regeneration.
abstract: Rapid functional epithelial regeneration on the luminal surface is essential when using artificial tracheal grafts to repair tracheal defects. In this study, we imposed human turbinate mesenchymal stromal cell (hTMSC) sheets for tracheal epithelial regeneration, and then assessed their potential as a new clinical cell source. In vitro, hTMSCs sheets showed high capacity to differentiate into tracheal epithelium. We fabricated a poly(ε-caprolactone) (PCL) tracheal graft by indirect three-dimensional (3D) printing technique and created a composite construct by transplanting the hTMSC sheets to its luminal surface of the tracheal graft, then applied this tissue-engineered tracheal graft to non-circumferential tracheal reconstruction in a rabbit model. 4 weeks after implantation, the luminal surface of tissue-engineered tracheal graft was covered by a mature and highly-ciliated epithelium, whereas tracheal grafts without hTMSC sheets were covered by only a thin, immature epithelium. Therefore, hTMSC sheets on the luminal surface of a tissue-engineered tracheal graft can accelerate the tracheal epithelial regeneration, and the tissue-engineered tracheal graft with hTMSC sheets provides a useful clinical alternative for tracheal epithelial regeneration.
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Otolaryngology and HNS, College of Medicine, The Catholic University of Korea, Republic of Korea.
authors.forename: Inn-Chul
authors.initials: IC
authors.lastname: Nam
authors.affiliation: Department of Otolaryngology and HNS, College of Medicine, The Catholic University of Korea, Republic of Korea.
authors.forename: Se-Hwan
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Department of Otolaryngology and HNS, College of Medicine, The Catholic University of Korea, Republic of Korea.
authors.forename: Choung-Soo
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jung
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Jinah
authors.initials: J
authors.lastname: Jang
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Hyungseok
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Yeongjin
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Otolaryngology and HNS, College of Medicine, The Catholic University of Korea, Republic of Korea.
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Otolaryngology and HNS, College of Medicine, The Catholic University of Korea, Republic of Korea; Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Republic of Korea. Electronic address: kswent@catholic.ac.kr.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea. Electronic address: dwcho@postech.ac.kr.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D009093:Mucus / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014132:Trachea / Q000502:physiology*; D014420:Turbinates / Q000166:cytology*
chemical_list: D011091:Polyesters; C016240:polycaprolactone
keywords: Human turbinate mesenchymal stromal cell (hTMSC) sheets; Indirect three-dimensional (3D) printing; Tissue engineering; Tracheal bellows graft; Tracheal epithelial regeneration