title: Differential Expression of Durum Wheat Gluten Proteome under Water Stress during Grain Filling.
abstract: Environmental stress during grain filling may affect wheat protein composition, thus influencing its final quality. A proteomic approach was used to evaluate changes in storage protein composition under water stress of two Italian durum wheat (Triticum turgidum ssp. durum) cultivars, Ciccio and Svevo. The high-molecular-weight glutenin region increased progressively in both cultivars and under two water regimens. The L48-35 region, corresponding to low-molecular-weight (LMW) glutenin subunits, increased slightly during grain development and decreased under water stress in both cultivars. In particular, an s-type LMW related to superior technological quality was down-expressed in the early-mid period in Svevo and in the mid-late period in Ciccio. Finally, the L<35 region, corresponding to gliadin-like proteins, decreased slightly during grain development and increased under stress in both cultivars. Several α-gliadins, associated with immunological potential, increased their expression under water stress, especially in Svevo in the early-mid stage of grain filling.
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Marcella Michela
authors.initials: MM
authors.lastname: Giuliani
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Palermo
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Michele Andrea
authors.initials: MA
authors.lastname: De Santis
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mentana
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Pompa
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Luigia
authors.initials: L
authors.lastname: Giuzio
authors.affiliation: §Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia (DAFNE), Università degli Studi della Tuscia, Via S. Camillo de Lellis snc, 01100 Viterbo, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Masci
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Centonze
authors.affiliation: †Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente (SAFE), Università degli Studi di Foggia, Via Napoli 25, 71122 Foggia, Italy.
authors.forename: Zina
authors.initials: Z
authors.lastname: Flagella
mesh_terms: D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D005983:Glutens / Q000032:analysis*; D007558:Italy; D010940:Plant Proteins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry / Q000254:growth & development*; D013312:Stress, Physiological / Q000502:physiology*; D014908:Triticum / Q000737:chemistry*; D014867:Water
chemical_list: D010940:Plant Proteins; D014867:Water; D005983:Glutens; C037302:glutenin
keywords: durum wheat; grain filling; proteomics; storage proteins; water stress