title: Piper betle-mediated synthesis, characterization, antibacterial and rat splenocyte cytotoxic effects of copper oxide nanoparticles.
abstract: The study reports a simple, inexpensive, and eco-friendly synthesis of copper oxide nanoparticles (CuONPs) using Piper betle leaf extract. Formation of CuONPs was confirmed by UV-visible spectroscopy at 280 nm. Transmission electron microscopy (TEM) images showed that the CuONPs were spherical, with an average size of 50-100 nm. The scanning electron microscopy (SEM)-energy dispersive spectroscopy (EDS) peak was observed approximately at 1 and 8 keV. The X-ray diffraction (XRD) studies indicated that the particles were crystalline in nature. CuONPs effectively inhibited the growth of phytopathogens Ralstonia solanacearum and Xanthomonas axonopodis. The cytotoxic effect of the synthesized CuONPs was analyzed using rat splenocytes. The cell viability was decreased to 94% at 300 μg/mL.
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Loganathan
authors.initials: L
authors.lastname: Praburaman
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Jum-Suk
authors.initials: JS
authors.lastname: Jang
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Govarthanan
authors.initials: G
authors.lastname: Muthusamy
authors.affiliation: b PG & Research Department of Biotechnology , Mahendra Arts and Science College , Kalippatti, Namakkal , Tamil Nadu , India.
authors.forename: Sengottaiyan
authors.initials: S
authors.lastname: Arumugam
authors.affiliation: c Department of Botany , Raja Duraisingam Government Arts College , Sivagangai , Tamil Nadu , India.
authors.forename: Koildhasan
authors.initials: K
authors.lastname: Manoharan
authors.affiliation: d National Institute of Crop Science, RDA , Iksan , Jeonbuk , South Korea.
authors.forename: Kwang-Min
authors.initials: KM
authors.lastname: Cho
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Cho
authors.initials: C
authors.lastname: Min
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Seralathan
authors.initials: S
authors.lastname: Kamala-Kannan
authors.affiliation: a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
authors.forename: Oh
authors.initials: O
authors.lastname: Byung-Taek
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003300:Copper / Q000737:chemistry*; D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D031721:Piper betle / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D043368:Ralstonia solanacearum / Q000254:growth & development*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D053532:Xanthomonas axonopodis / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D003603:Cytotoxins; D010936:Plant Extracts; D003300:Copper; C030973:cupric oxide
keywords: antibacterial activity; cytotoxicity; hemolysis; phytopathogens; piper betle