title: Association of gene polymorphisms in ABO blood group chromosomal regions and menstrual disorders.
abstract: This study aimed to investigate whether single nucleotide polymorphisms (SNPs) located near the gene of the ABO blood group play an important role in the genetic aetiology of menstrual disorders (MDs). Polymerase chain reaction-ligase detection reaction technology was used to detect eight SNPs near the ABO gene location on the chromosomes in 250 cases of MD and 250 cases of normal menstruation. The differences in the distribution of each genotype, as well as the allele frequency in the normal and control groups, were analysed using Pearson's χ2test to search for disease-associated loci. SHEsis software was used to analyse the linkage disequilibrium and haplotype frequencies and to inspect the correlation between haplotypes and the disease. Compared with the control group, the experimental group exhibited statistically significant differences in the genotype distribution frequencies of the rs657152 locus of the ABO blood group gene and the rs17250673 locus of the tumour necrosis factor cofactor 2 (TRAF2) gene, which is located downstream of the ABO gene. The allele distribution frequencies of rs657152 and rs495828 loci in the ABO blood group gene exhibited significant differences between the groups. Dominant and recessive genetic model analysis of each locus revealed that the experimental group exhibited statistically significant differences from the control group in the genotype distribution frequencies of rs657152 and rs495828 loci, respectively. These results indicate that the ABO blood group gene and TRAF2 gene may be a cause of MDs.
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Gui-Lian
authors.initials: GL
authors.lastname: Kong
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Su
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Lv
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Huang
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Rui-Zhi
authors.initials: RZ
authors.lastname: Zheng
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Quan-Zhong
authors.initials: QZ
authors.lastname: Li
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Yuan
authors.affiliation: Department of Endocrinology, People's Hospital of Zhengzhou University (Henan Province People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhao
keywords: ABO blood group gene; gene polymorphism; menstrual disorders