title: Self-reported student confidence in troubleshooting ability increases after completion of an inquiry-based PCR practical.
abstract: Inquiry-based learning (IBL) activities are complementary to the processes of laboratory discovery, as both are focused on producing new findings through research and inquiry. Here, we describe the results of student surveys taken pre- and postpractical to an IBL undergraduate practical on PCR. Our analysis focuses primarily student perceptions of knowledge acquisition and their ability to troubleshoot problems. The survey results demonstrate significant self-reported gains in knowledge related to DNA structure and PCR, and an increase in confidence with "troubleshooting problems during scientific experiments." We conclude that the IBL-based approach that combines PCR primer design with wet laboratory experimentation using student-designed primers, provides students a sense of confidence by imparting workplace and research skills that are integral to diverse forms and applications of laboratory practices.
authors.affiliation: School of Health Sciences, Faculty of Health, University of Tasmania, Tasmania, Australia.
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Cook
authors.affiliation: School of Health Sciences, Faculty of Health, University of Tasmania, Tasmania, Australia.
authors.forename: Elizabeth T
authors.initials: ET
authors.lastname: Snow
authors.affiliation: School of Health Sciences, Faculty of Health, University of Tasmania, Tasmania, Australia.
authors.forename: Henrica
authors.initials: H
authors.lastname: Binns
authors.affiliation: School of Social Sciences, Faculty of Arts, University of Tasmania, Tasmania, Australia.
authors.forename: Peta S
authors.initials: PS
authors.lastname: Cook
mesh_terms: D003479:Curriculum; D017931:DNA Primers; D004521:Educational Measurement / Q000379:methods*; D005260:Female; D006801:Humans; D019359:Knowledge; D007858:Learning; D008297:Male; D016133:Polymerase Chain Reaction; D012106:Research / Q000193:education*; D012649:Self Concept; D057566:Self Report; D013334:Students / Q000523:psychology*; D055815:Young Adult
chemical_list: D017931:DNA Primers
keywords: inquiry-based learning; polymerase chain reaction; student self confidence; undergraduate