title: Establishment and characterization of a novel orthotopic mouse model for human uterine sarcoma with different metastatic potentials.
abstract: Uterine sarcomas are rare and aggressive gynecologic tumors with a poor prognosis because of recurrence and metastasis. However, the mechanisms of uterine sarcoma metastasis are largely unknown. To investigate this mechanism, we developed a novel uterine sarcoma tissue-derived orthotopic and metastatic model in KSN nude mice using a green fluorescent protein stably expressed uterine sarcoma cell line, MES-SA. Histological analysis showed that all orthotopic primary tumors were undifferentiated sarcoma. Primary tumors were characterized by high (18)F-fluorodeoxyglucose uptake with a positive correlation to the number of pulmonary metastases. In addition, we generated uterine sarcoma cell sublines with high or low metastatic potentials by serial in vivo selection. Microarray analysis between orthotopic tumors with high and low metastatic potentials revealed differential expression of genes related to cell proliferation and migration (TNNT1, COL1A2, and ZIC1). Our model would be useful to compensate for the limited clinical cases of uterine sarcoma and to investigate the molecular mechanisms of metastatic uterine sarcoma.
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan; Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kawabe
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan; Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan. Electronic address: mizutani@u-fukui.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Ishikane
authors.affiliation: Biomedical Imaging Research Center, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Miguel Ernesto
authors.initials: ME
authors.lastname: Martinez
authors.affiliation: Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan; Biomedical Imaging Research Center, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kiyono
authors.affiliation: National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Miura
authors.affiliation: National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoda
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan; Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Imamichi
authors.affiliation: National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan; Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Miyamoto
authors.affiliation: Organization for Life Science Advancement Programs, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan; Department of Obstetrics and Gynecology, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D008819:Mice, Nude; D049268:Positron-Emission Tomography; D012509:Sarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D014157:Transcription Factors / Q000378:metabolism; D020107:Troponin T / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D014157:Transcription Factors; D020107:Troponin T; C492123:ZIC1 protein, human; C047515:2-fluoro-2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate
keywords: GFP; Gene expression; Histological analysis; MES-SA; PET imaging