title: Methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and diabetic nephropathy susceptibility in patients with type 2 diabetes mellitus.
abstract: Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme that regulates nucleotide synthesis and DNA methylation. The MTHFR C677T gene polymorphism (rs1801133), a C → T transition at nucleotide 677 in exon 4, is a common gene variant of MTHFR and has been implicated in diabetic nephropathy, albeit with inconsistent results. Here, we performed a meta-analysis to assess the common effect size of this polymorphism on DN susceptibility. Case-control studies on the association of the MTHFR C677T gene polymorphism with DN risk were retrieved from databases up to August 1, 2013, and eligible studies were recruited into the meta-analysis and further analyzed. Of 132 studies, 33 were identified as suitable for this analysis. The results showed that T allele and TT genotype were distinctly associated with DN susceptibility in the overall population and Asians, and might be a risk factor in Caucasians and Africans (T allele: Overall population: p < 0.00001, Asians: p = 0.0002, Caucasians: p = 0.02, Africans: p < 0.00001; TT genotype: Overall population: p < 0.00001, Asians: p = 0.0003, Caucasians: p = 0.008, Africans: p = 0.0003). Furthermore, the analysis suggested that the CC genotype might play a protective role against DN onset in patients with type 2 diabetes for the overall population, Asians, Caucasian and Africans. However, due to the limited sample size in the African population, these results should be interpreted with care. In conclusion, the MTHFR C677T T allele or TT genotype might be a significant genetic molecular marker to determine the risk of DN in patients with type 2 diabetes and help to develop suitable disease prevention and management strategies.
authors.affiliation: a Department of Nephrology , The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou , China .
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
authors.affiliation: b Cellular Stress and Ageing Program, Bionanoscience and Bio-Imaging Program, Bio&Nano-Solutions , Düsseldorf , Germany , and.
authors.forename: Gregor P C
authors.initials: GP
authors.lastname: Drummen
authors.affiliation: a Department of Nephrology , The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou , China .
authors.forename: Zong-Pei
authors.initials: ZP
authors.lastname: Jiang
authors.affiliation: c Department of Nephrology , Huadu District People's Hospital, Southern Medical University , Guangzhou , China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Li
mesh_terms: D000483:Alleles; D015415:Biomarkers; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D003928:Diabetic Nephropathies / Q000175:diagnosis / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044469:Racial Groups / Q000235:genetics*; D012307:Risk Factors
chemical_list: D015415:Biomarkers; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: C677T; MTHFR; diabetic nephropathy; gene polymorphism; meta-analysis; methylenetetrahydrofolate reductase