title: Denaturation and Oxidative Stability of Hemp Seed (Cannabis sativa L.) Protein Isolate as Affected by Heat Treatment.
abstract: The present study investigated the impact of heat treatments on the denaturation and oxidative stability of hemp seed protein during simulated gastrointestinal digestion (GID). Heat-denatured hemp protein isolate (HPI) solutions were prepared by heating HPI (2 mg/ml, pH 6.8) to 40, 60, 80 and 100 °C for 10 min. Heat-induced denaturation of the protein isolates was monitored by polyacrylamide gel electrophoresis. Heating HPI at temperatures above 80 °C significantly reduced solubility and led to the formation of large protein aggregates. The isolates were then subjected to in vitro GID and the oxidative stability of the generated peptides was investigated. Heating did not significantly affect the formation of oxidation products during GID. The results suggest that heat treatments should ideally remain below 80 °C if heat stability and solubility of HPI are to be preserved.
authors.affiliation: Natural Products Group, Rowett Institute of Nutrition and Health, University of Aberdeen, AB21 9SB, Scotland, UK, v.raikos@abdn.ac.uk.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Raikos
authors.forename: Garry
authors.initials: G
authors.lastname: Duthie
authors.forename: Viren
authors.initials: V
authors.lastname: Ranawana
mesh_terms: D002188:Cannabis / Q000737:chemistry*; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel; D041981:Gastrointestinal Tract; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010455:Peptides / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011489:Protein Denaturation; D012639:Seeds / Q000737:chemistry*; D012995:Solubility; D012996:Solutions
chemical_list: D004044:Dietary Proteins; D010455:Peptides; D010940:Plant Proteins; D012996:Solutions