title: In Vivo Remote Control of Reactions in Caenorhabditis elegans by Using Supramolecular Nanohybrids of Carbon Nanotubes and Liposomes.
abstract: A supramolecular nanohybrid based on carbon nanotubes and liposomes that is highly biocompatible and capable of permeation through cells is described. The nanohybrid can be loaded with a variety of functional molecules and is structurally controlled by near-infrared laser irradiation for the release of molecules from the nanohybrids in a targeted manner via microscopy. We implemented the controlled release of molecules from the nanohybrids and demonstrated remote regulation of the photoinduced nanohybrid functions. As a proof of principle, nanohybrids loaded with amiloride were successfully used in the spatiotemporally targeted blocking of amiloride-sensitive mechanosensory neurons in living Caenorhabditis elegans. Our prototype could inspire new designs for biomimetic parasitism and symbiosis, and biologically active nanorobots for the higher-level manipulation of organisms.
authors.affiliation: Department of Materials and Chemistry, Nanomaterial Research Institute (NMRI), National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565 (Japan). e-miyako@aist.go.jp.
authors.forename: Eijiro
authors.initials: E
authors.lastname: Miyako
authors.affiliation: Department of Materials and Chemistry, Nanomaterial Research Institute (NMRI), National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565 (Japan).
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Chechetka
authors.affiliation: Department of Life Science and Biotechnology, Biomedical Research Institute (BRI) & DAILAB, AIST, Central 6, 1-1-1 Higashi, Tsukuba 305-8566 (Japan).
authors.forename: Motomichi
authors.initials: M
authors.lastname: Doi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 (Japan).
authors.forename: Eiji
authors.initials: E
authors.lastname: Yuba
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 (Japan).
authors.forename: Kenji
authors.initials: K
authors.lastname: Kono
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D008081:Liposomes; D037742:Nanotubes, Carbon
keywords: carbon nanotubes; controlled delivery; liposomes; nanotechnology; neurons