title: Stability of engineered nanomaterials in complex aqueous matrices: Settling behaviour of CeO2 nanoparticles in natural surface waters.
abstract: The stability of engineered nanoparticles (ENPs) in complex aqueous matrices is a key determinant of their fate and potential toxicity towards the aquatic environment and human health. Metal oxide nanoparticles, such as CeO2 ENPs, are increasingly being incorporated into a wide range of industrial and commercial applications, which will undoubtedly result in their (unintentional) release into the environment. Hereby, the behaviour and fate of CeO2 ENPs could potentially serve as model for other nanoparticles that possess similar characteristics. The present study examined the stability and settling of CeO2 ENPs (7.3±1.4 nm) as well as Ce(3+) ions in 10 distinct natural surface waters during 7d, under stagnant and isothermal experimental conditions. Natural water samples were collected throughout Flanders (Belgium) and were thoroughly characterized. For the majority of the surface waters, a substantial depletion (>95%) of the initially added CeO2 ENPs was observed just below the liquid surface of the water samples after 7d. In all cases, the reduction was considerably higher for CeO2 ENPs than for Ce(3+) ions (<68%). A first-order kinetics model was able to describe the observed time-dependant removal of both CeO2 ENPs (R(2)≥0.998) and Ce(3+) ions (R(2)≥0.812) from the water column, at least in case notable sedimentation occurred over time. Solution-pH appeared to be a prime parameter governing nanoparticle colloidal stability. Moreover, the suspended solids (TSS) content also seemed to be an important factor affecting the settling rate and residual fraction of CeO2 ENPs as well as Ce(3+) ions in natural surface waters. Correlation results also suggest potential association and co-precipitation of CeO2 ENPs with aluminium- and iron-containing natural colloidal material. The CeO2 ENPs remained stable in dispersion in surface water characterized by a low pH, ionic strength (IS), and TSS content, indicating the eventual stability and settling behaviour of the nanoparticles was likely determined by a combination of physicochemical parameters. Finally, ionic release from the nanoparticle surface was also examined and appeared to be negligible in all of the tested natural waters.
authors.affiliation: Laboratory of Analytical Chemistry and Applied Ecochemistry, Department of Applied Analytical and Physical Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium. Electronic address: Frederik.VanKoetsem@UGent.be.
authors.forename: Frederik
authors.initials: F
authors.lastname: Van Koetsem
authors.affiliation: Laboratory of Analytical Chemistry and Applied Ecochemistry, Department of Applied Analytical and Physical Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium.
authors.forename: Simon
authors.initials: S
authors.lastname: Verstraete
authors.affiliation: Particle and Interfacial Technology Group, Department of Applied Analytical and Physical Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: Van der Meeren
authors.affiliation: Laboratory of Analytical Chemistry and Applied Ecochemistry, Department of Applied Analytical and Physical Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium. Electronic address: Gijs.DuLaing@UGent.be.
authors.forename: Gijs
authors.initials: G
authors.lastname: Du Laing
mesh_terms: D001530:Belgium; D002563:Cerium / Q000032:analysis*; D004784:Environmental Monitoring; D005618:Fresh Water / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D002563:Cerium; C030583:ceric oxide
keywords: Cerium dioxide; Nanoparticles; Sedimentation; Stability; Surface water