title: The role of Pin1 protein in aging of human tendon stem/progenitor cells.
abstract: Aging of tendon stem/progenitor cells (TSPCs) can lead to tissue degeneration and subsequent injury. However, the molecular mechanisms controlling TSPC aging are not completely understood. In the present study, we investigated the role of Pin1 in aging of human TSPCs. Pin1 mRNA and protein expression levels were significantly decreased during prolonged in vitro culture of human TSPCs. Furthermore, overexpression of Pin1 delayed the progression of cellular senescence, as confirmed by downregulation of senescence-associated β-galactosidase, increased telomerase activity and decreased levels of the senescence marker, p16(INK4A). Conversely, Pin1 siRNA transfection promoted senescence in TSPCs. In addition, miR-140-5p regulated Pin1 expression at the translational level via directly targeting its 3'UTR. Our results collectively demonstrate that Pin1 acts as an important regulator of TSPC aging.
authors.affiliation: Department of Orthopaedics Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Junpeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Tao
authors.affiliation: Department of Orthopaedics Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan, China. Electronic address: qingwangchina@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan, China. Electronic address: liuximing2010@163.com.
authors.forename: Ximing
authors.initials: X
authors.lastname: Liu
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000502:physiology; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*; D013710:Tendons / Q000166:cytology*
chemical_list: D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase
keywords: Aging; Pin1; Tendon stem/progenitor cells; miR-140-5p