title: Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation.
abstract: Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform ("CherryOne") capable of real-time monitoring, metering, and control of the dynamic liquid-liquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based phase metering apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real-time display of stationary phase retention (Sf) and eluent partition coefficients (K), which represent critical parameters of CCS reproducibility. When coupled with the elution-extrusion operational mode (EECCC), automated Sf and K determination empowers untargeted and targeted applications ranging from metabolomic analysis to preparative purifications.
authors.forename: Guido F
authors.initials: GF
authors.lastname: Pauli
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Pro
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: Lucas R
authors.initials: LR
authors.lastname: Chadwick
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: Thomas
authors.initials: T
authors.lastname: Burdick
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: Luke
authors.initials: L
authors.lastname: Pro
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: Warren
authors.initials: W
authors.lastname: Friedl
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: Nick
authors.initials: N
authors.lastname: Novak
authors.affiliation: §Cherry Instruments, 1134 West Granville Avenue, Suite 1109, Chicago, Illinois 60660, United States.
authors.forename: John
authors.initials: J
authors.lastname: Maltby
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.affiliation: ⊥Physical Sciences Department, Dominican University, River Forest, Illinois 60305, United States.
authors.forename: J Brent
authors.initials: JB
authors.lastname: Friesen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D027582:Humulus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry*; D013997:Time Factors
chemical_list: D010936:Plant Extracts