title: Characterization of Changes in Global Genes Expression in the Distal Colon of Loperamide-Induced Constipation SD Rats in Response to the Laxative Effects of Liriope platyphylla.
abstract: To characterize the changes in global gene expression in the distal colon of constipated SD rats in response to the laxative effects of aqueous extracts of Liriope platyphylla (AEtLP), including isoflavone, saponin, oligosaccharide, succinic acid and hydroxyproline, the total RNA extracted from the distal colon of AEtLP-treated constipation rats was hybridized to oligonucleotide microarrays. The AEtLP treated rats showed an increase in the number of stools, mucosa thickness, flat luminal surface thickness, mucin secretion, and crypt number. Overall, compared to the controls, 581 genes were up-regulated and 216 genes were down-regulated by the constipation induced by loperamide in the constipated rats. After the AEtLP treatment, 67 genes were up-regulated and 421 genes were down-regulated. Among the transcripts up-regulated by constipation, 89 were significantly down-regulated and 22 were recovered to the normal levels by the AEtLP treatment. The major genes in the down-regulated categories included Slc9a5, klk10, Fgf15, and Alpi, whereas the major genes in the recovered categories were Cyp2b2, Ace, G6pc, and Setbp1. On the other hand, after the AEtLP treatment, ten of these genes down-regulated by constipation were up-regulated significantly and five were recovered to the normal levels. The major genes in the up-regulated categories included Serpina3n, Lcn2 and Slc5a8, whereas the major genes in the recovered categories were Tmem45a, Rerg and Rgc32. These results indicate that several gene functional groups and individual genes as constipation biomarkers respond to an AEtLP treatment in constipated model rats.
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan, 609-735, Korea.
authors.forename: So Hae
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Moon Hwa
authors.initials: MH
authors.lastname: Kwak
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Go
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Koh
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Song
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Sung
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan, 609-735, Korea.
authors.forename: Hee Seob
authors.initials: HS
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Chungju, 361-763, Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D003248:Constipation / Q000139:chemically induced / Q000188:drug therapy*; D015536:Down-Regulation / Q000187:drug effects*; D054769:G-Protein-Coupled Receptor Kinase 2; D054368:Laxatives / Q000627:therapeutic use*; D031424:Liriope Plant / Q000737:chemistry* / Q000378:metabolism; D008139:Loperamide / Q000633:toxicity; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D054368:Laxatives; D010936:Plant Extracts; D008139:Loperamide; D020794:Receptor Protein-Tyrosine Kinases; C080577:muscarinic receptor kinase; D054769:G-Protein-Coupled Receptor Kinase 2