title: (1)H, (15)N and (13)C backbone resonance assignments of the N-terminal, tandem KH domains of human hnRNP E1.
abstract: Heterogeneous nuclear ribonucleoproteins (hnRNPs) can be divided into subgroups based on their RNA-binding characteristics. One subgroup in mammalian cells are the Poly(C)-binding proteins (PCBPs) comprised of hnRNP K/J and hnRNP E1-4 [the latter also known as PCBP 1-4 or α-complex proteins (α-CP) 1-4]. Each subgroup member has three K homology (KH) nucleic acid-binding domains. Individual KH domains bind short single-stranded (ss), poly-pyrimidine-rich nucleic acid sequences with rather weak affinity. In this study, we report the (1)H, (13)C and (15)N backbone resonance assignments of the first and second KH domains of hnRNP E1, which plays a pivotal role in posttranscriptional and translational regulation of RNA targets. Our NMR assignments lay the foundation for a detailed investigation of the dynamic cooperation of the tandem KH1 and KH2 domains to bind nucleic acids.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, 29425, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Nutrition, University of North Carolina at Chapel Hill/Nutrition Research Institute, Room 3151, 500 Laureate Way, Kannapolis, NC, 28081, USA. mirko_hennig@unc.edu.
authors.forename: Mirko
authors.initials: M
authors.lastname: Hennig
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000737:chemistry*; D006801:Humans; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D016601:RNA-Binding Proteins
chemical_list: D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009587:Nitrogen Isotopes; C094604:PCBP1 protein, human; D016601:RNA-Binding Proteins
keywords: Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1); K homology (KH) domain; Nucleic acid binding; Poly(C)-binding protein 1 (PCBP1)