title: Bioorthogonal Oxime Ligation MediatedIn VivoCancer Targeting.
abstract: Current cancer targeting relying on specific biological interaction between cell surface antigen and respective antibody or its analogue has proven to be effective in the treatment of different cancers; however, this strategy has its own limitations, such as heterogeneity of cancer cells and immunogenicity of the biomacromolecule binding ligands. Bioorthogonal chemical conjugation has emerged as an attractive alternative to biological interaction for in vivo cancer targeting. Here, we report an in vivo cancer targeting strategy mediated by bioorthogonal oxime ligation. Oxyamine group, the artificial target, is introduced onto 4T1 murine breast cancer cells through liposome delivery and fusion. Poly(ethylene glycol) -polylactide (PEG-PLA) nanoparticle (NP) is surface-functionalized with aldehyde groups as targeting ligands. The improvedin vivocancer targeting of PEG-PLA NPs is achieved through specific and efficient chemical reaction between the oxyamine and aldehyde groups.
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA. ; Tel: +1 217-244-3924.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA. ; Tel: +1 217-244-3924.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA. ; Tel: +1 217-244-3924.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Science, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA. ; Tel: +1 217-244-3924.
authors.forename: Kaimin
authors.initials: K
authors.lastname: Cai
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA. ; Tel: +1 217-244-3924.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Yen
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Lawrence W
authors.initials: LW
authors.lastname: Dobrucki
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA. ; Tel: +1 217-244-3924 ; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Cheng