title: Hydrogen sulfide protects spinal cord and induces autophagy via miR-30c in a rat model of spinal cord ischemia-reperfusion injury.
abstract: BACKGROUND
  Hydrogen sulfide (H2S), a novel gaseous mediator, has been recognized as an important neuromodulator and neuroprotective agent in the nervous system. The present study was undertaken to study the effects of exogenous H2S on ischemia/reperfusion (I/R) injury of spinal cord and the underlying mechanisms.
  
  
  METHODS
  The effects of exogenous H2S on I/R injury were examined by using assessment of hind motor function, spinal cord infarct zone by Triphenyltetrazolium chloride (TTC) staining. Autophagy was evaluated by expressions of Microtubule associated protein 1 light chain 3 (LC3) and Beclin-1 which were determined by using Quantitative Real-Time PCR and Western blotting, respectively.
  
  
  RESULTS
  Compared to I/R injury groups, H2S pretreatment had reduced spinal cord infarct zone, improved hind motor function in rats. Quantitative Real-Time PCR or Western blotting results showed that H2S pretreatment also downregulated miR-30c expression and upregulated Beclin-1 and LC3II expression in spinal cord. In vitro, miR-30c was showed to exert negative effect on Beclin-1 expression by targeting its 3'UTR in SY-SH-5Y cells treated with Oxygen, Glucose Deprivation (OGD). In rat model of I/R injury, pretreatment of pre-miR-30c or 3-MA (an inhibitor for autophagy) can abrogated spinal cord protective effect of H2S.
  
  
  CONCLUSIONS
  H2S protects spinal cord and induces autophagy via miR-30c in a rat model of spinal cord hemia-reperfusion injury.
authors.affiliation: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004, China. LeiLi1100@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Hong-kun
authors.initials: HK
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
authors.forename: Yun-peng
authors.initials: YP
authors.lastname: Li
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Guo
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D006862:Hydrogen Sulfide / Q000008:administration & dosage*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D018696:Neuroprotective Agents / Q000008:administration & dosage; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D018696:Neuroprotective Agents; D006862:Hydrogen Sulfide