title: A histomorphometric assessment of collagen-stabilized anorganic bovine bone mineral in maxillary sinus augmentation - a randomized controlled trial in sheep.
abstract: OBJECTIVE
  To histomorphometrically compare the use of collagen-stabilized anorganic bovine bone (ABBM-C) (test) to anorganic bovine bone + autogenous bone (ABBM + AB) (control) in maxillary sinus augmentation.
  
  
  METHODS
  Nine sheep underwent bilateral sinus augmentation. Each sinus was randomized to receive either control or test bone graft. Three animals were sacrificed at 8 weeks, and six animals were sacrificed at 16 weeks post-grafting. The 18 sinuses were processed for histomorphometry, which assessed the area fraction of new bone (%NB), residual graft (%RG) and soft tissue components (% STM), as well as graft particle osseointegration (% OI), within three zones equally distributed from the augmented sinus floor.
  
  
  RESULTS
  At week 16, a significant increase in %NB was evident across all three zones in the control group when compared to week 8. A significantly greater %NB was evident in the control group when compared to the test group in zones 2 (P < 0.001) and 3 (P < 0.001). There was a significant increase in %OI in week 16 when compared to week 8 across all three zones in the control group (P < 0.001). %OI in the control group was significantly greater across all three zones when compared to the test group at week 16 (P < 0.001). Zone was found to be a significant main effect (P < 0.001) that was independent of time and treatment with decreasing %OI in distant zones. %RG did not significantly change with time for both groups. There was a significant reduction in %ST in week 16 when compared to week 8 across all three zones in the control group (P < 0.001). %ST in the test group was significantly greater across all zones when compared to the control group at week 16 (P < 0.001).
  
  
  CONCLUSIONS
  Both groups exhibited very similar histomorphometric measurements in the zones proximal to the resident sinus wall. The % NB and % OI were greatest in the zones proximal to resident bony walls and gradually decreased as the distance from the proximal walls increased. There was greater % NB and % OI in the control group when compared to the test group in the distant zone.
authors.affiliation: School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Southport, Qld, Australia.
authors.forename: Jamil
authors.initials: J
authors.lastname: Alayan
authors.affiliation: Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Qld, Australia.
authors.forename: Cedryck
authors.initials: C
authors.lastname: Vaquette
authors.affiliation: Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Qld, Australia.
authors.forename: Siamak
authors.initials: S
authors.lastname: Saifzadeh
authors.affiliation: Institute for Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Qld, Australia.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Hutmacher
authors.affiliation: School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Southport, Qld, Australia.
authors.forename: Saso
authors.initials: S
authors.lastname: Ivanovski
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D003094:Collagen / Q000494:pharmacology*; D008443:Maxillary Sinus / Q000473:pathology* / Q000601:surgery; D008903:Minerals / Q000494:pharmacology*; D023421:Models, Animal; D012756:Sheep; D059546:Sinus Floor Augmentation / Q000379:methods*
chemical_list: C077540:Bio-Oss; D008903:Minerals; D003094:Collagen
keywords: animal model; bone grafting; bone regeneration; bone substitute; histomorphometry; maxillary sinus augmentation