title: Zn/Ag micro-galvanic couples formed on titanium and osseointegration effects in the presence of S. aureus.
abstract: Titanium implants possessing simultaneous osseointegration and antibacterial ability are desirable. In this work, three types of Zn/Ag micro-galvanic couples are fabricated on titanium by plasma immersion ion implantation to investigate the osseointegration and antibacterial effects as well as the involved mechanisms. The in vitro findings disclose enhanced proliferation, osteogenic differentiation, and gene expressions of the rat bone mesenchymal stem cells (rBMSCs), as well as good antibacterial ability on all three micro-galvanic couples. Excellent antimicrobial ability is also observed in vivo and the micro-CT and histological results reveal notable osseointegration in vivo despite the presence of bacteria. The Zn/Ag micro-galvanic couple formed on Zn/Ag dual-ion co-implanted titanium shows the best osseointegration as well as good antibacterial properties in vivo obtained from a rabbit tibia model. The difference among the three Zn/Ag micro-galvanic couples can be ascribed to the contact between the Ag NPs and Zn film, which affects the corrosion process. Our results indicate that the biological behavior can be controlled by the corrosion process of the Zn/Ag micro-galvanic couples.
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Jin
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qin
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Cao
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
authors.forename: Yaochao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. Electronic address: zhangxianl197826@163.com.
authors.forename: Xianlong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: xyliu@mail.sic.ac.cn.
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D019736:Prostheses and Implants; D011817:Rabbits; D051381:Rats; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D018786:Bone Substitutes; D012834:Silver; D014025:Titanium; D015032:Zinc
keywords: Antibacterial ability; Micro-galvanic couple; Osseointegration; Silver; Titanium; Zinc