title: Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer.
abstract: Breast cancer is the leading cause of cancer-related mortality for females worldwide.1Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in anMMTV-ErbB2mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplasticMMTV-ErbB2female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potential even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).
authors.affiliation: Department of Pathology, University of Iowa, Iowa City, IA 52242-1109 ; Medical Science Training Program, University of Iowa, Iowa City, IA 52242-1109 ; Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA 52242-1109 ; Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Borcherding
authors.affiliation: Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Bormann
authors.affiliation: Department of Pathology, University of Iowa, Iowa City, IA 52242-1109 ; Molecular and Cellular Biology Program, University of Iowa, Iowa City, IA 52242-1109.
authors.forename: David
authors.initials: D
authors.lastname: Kusner
authors.affiliation: Department of Pathology, University of Iowa, Iowa City, IA 52242-1109 ; Immunology Program, University of Iowa, Iowa City, IA 52242-1109.
authors.forename: Ryan
authors.initials: R
authors.lastname: Kolb
authors.affiliation: Department of Pathology, University of Iowa, Iowa City, IA 52242-1109 ; Medical Science Training Program, University of Iowa, Iowa City, IA 52242-1109 ; Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA 52242-1109 ; Molecular and Cellular Biology Program, University of Iowa, Iowa City, IA 52242-1109 ; Immunology Program, University of Iowa, Iowa City, IA 52242-1109 ; Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109.
authors.forename: Weizhou
authors.initials: W
authors.lastname: Zhang
keywords: Breast cancer; luminal progenitors; mammary stem cells; myoepithelial cells; tumor-initiating cells