title: Enteric epithelial cells support growth of Hymenolepis diminuta in vitro and trigger TH2-promoting events in a species-specific manner.
abstract: Knowledge of the gut epithelium in modulating immune responses to cestode parasites is scant. Hymenolepis diminuta causes no damage to its rodent host and is expelled from mice. Hymenolepis diminuta (scolex+2cm strobila) was cultured with rat (IEC6), human (T84) or mouse (IEC4) epithelial cell lines: all promoted worm survival, but those cultured on IEC6 (rat is a permissive host) were the healthiest. In contrast, production of Th2 polarising cytokines, IL-17e, IL-33 and TSLP, was greatest in IEC4 cells, less in T84 epithelia and almost negligible in IEC6 cells. Thus, the enteric epithelium is a key determinant of the response to infection with H. diminuta and is predictive of host permissiveness.
authors.affiliation: Gastrointestinal Research Group and Inflammation Research Network, Department of Physiology and Pharmacology, Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Fernando
authors.initials: F
authors.lastname: Lopes
authors.affiliation: Gastrointestinal Research Group and Inflammation Research Network, Department of Physiology and Pharmacology, Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Reyes
authors.affiliation: Gastrointestinal Research Group and Inflammation Research Network, Department of Physiology and Pharmacology, Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Arthur
authors.initials: A
authors.lastname: Wang
authors.affiliation: Gastrointestinal Research Group and Inflammation Research Network, Department of Physiology and Pharmacology, Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Leung
authors.affiliation: Gastrointestinal Research Group and Inflammation Research Network, Department of Physiology and Pharmacology, Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address: dmckay@ucalgary.ca.
authors.forename: Derek M
authors.initials: DM
authors.lastname: McKay
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology* / Q000469:parasitology*; D006801:Humans; D048190:Hymenolepis diminuta / Q000254:growth & development* / Q000276:immunology*; D007113:Immunity, Innate; D020381:Interleukin-17 / Q000378:metabolism; D000067596:Interleukin-33 / Q000378:metabolism; D051379:Mice; D051381:Rats; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D000067596:Interleukin-33; D000094632:Thymic Stromal Lymphopoietin
keywords: Cestode; IL-25; IL-33; In vitro culture; TSLP