title: Recognition of microbial glycans by human intelectin-1.
abstract: The glycans displayed on mammalian cells can differ markedly from those on microbes. Such differences could, in principle, be 'read' by carbohydrate-binding proteins, or lectins. We used glycan microarrays to show that human intelectin-1 (hIntL-1) does not bind known human glycan epitopes but does interact with multiple glycan epitopes found exclusively on microbes: β-linked D-galactofuranose (β-Galf), D-phosphoglycerol-modified glycans, heptoses, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). The 1.6-Å-resolution crystal structure of hIntL-1 complexed with β-Galf revealed that hIntL-1 uses a bound calcium ion to coordinate terminal exocyclic 1,2-diols. N-acetylneuraminic acid (Neu5Ac), a sialic acid widespread in human glycans, has an exocyclic 1,2-diol but does not bind hIntL-1, probably owing to unfavorable steric and electronic effects. hIntL-1 marks only Streptococcus pneumoniae serotypes that display surface glycans with terminal 1,2-diol groups. This ligand selectivity suggests that hIntL-1 functions in microbial surveillance.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Darryl A
authors.initials: DA
authors.lastname: Wesener
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Kittikhun
authors.identifier: 0000000271902955
authors.initials: K
authors.lastname: Wangkanont
authors.affiliation: 1] Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California, USA. [2] Department of Chemical Physiology, Scripps Research Institute, La Jolla, California, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: McBride
authors.affiliation: 1] Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA. [2] Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Kraft
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Heather L
authors.initials: HL
authors.lastname: Hodges
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Lucas C
authors.initials: LC
authors.lastname: Zarling
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Splain
authors.affiliation: 1] Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA. [2] Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.affiliation: 1] Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA. [2] Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
authors.affiliation: 1] Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California, USA. [2] Department of Chemical Physiology, Scripps Research Institute, La Jolla, California, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Katrina T
authors.initials: KT
authors.lastname: Forest
authors.affiliation: 1] Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA. [2] Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Kiessling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D018360:Crystallography, X-Ray; D016207:Cytokines / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry* / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008024:Ligands; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D016207:Cytokines; D000939:Epitopes; D058851:GPI-Linked Proteins; C432397:ITLN1 protein, human; D037102:Lectins; D008024:Ligands; D008070:Lipopolysaccharides; D009005:Monosaccharides; D011134:Polysaccharides