title: A new anti-inflammatory β-carboline alkaloid from the hairy-root cultures of Eurycoma longifolia.
abstract: One new β-carboline alkaloid 7-methoxy-(9H-β-carbolin-1-il)-(E)-1-propenoic acid (1) together with 9-methoxycanthin-6-one (2) and 9-hydroxycanthin-6-one (3) were isolated from the hairy-root cultures of Eurycoma longifolia. The effects of these compounds on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells were investigated. Compound 1 strongly inhibited the production of NO while 2 and 3 having weak or inactive effect. Consistently, compound 1 decreased the expression of cyclooxygenase-2 and inducible nitric oxide synthase.
authors.affiliation: a Institute of Biotechnology , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet, Cau Giay, Hanoi , Vietnam.
authors.forename: Pham Bich
authors.initials: PB
authors.lastname: Ngoc
authors.affiliation: b Institute of Marine Biochemistry , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet Cau Giay, Hanoi , Vietnam.
authors.forename: Thanh Binh
authors.initials: TB
authors.lastname: Pham
authors.affiliation: b Institute of Marine Biochemistry , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet Cau Giay, Hanoi , Vietnam.
authors.forename: Hai Dang
authors.initials: HD
authors.lastname: Nguyen
authors.affiliation: a Institute of Biotechnology , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet, Cau Giay, Hanoi , Vietnam.
authors.forename: Thu Trang
authors.initials: TT
authors.lastname: Tran
authors.affiliation: a Institute of Biotechnology , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet, Cau Giay, Hanoi , Vietnam.
authors.forename: Hoang Ha
authors.initials: HH
authors.lastname: Chu
authors.affiliation: b Institute of Marine Biochemistry , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet Cau Giay, Hanoi , Vietnam.
authors.forename: Van Minh
authors.initials: VM
authors.lastname: Chau
authors.affiliation: c Department of Biochemistry , College of Natural Sciences, Kangwon National University , Chuncheon , Gangwon-Do 200-701 , Korea.
authors.forename: Jeong-Hyung
authors.initials: JH
authors.lastname: Lee
authors.affiliation: b Institute of Marine Biochemistry , Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet Cau Giay, Hanoi , Vietnam.
authors.forename: Tien Dat
authors.initials: TD
authors.lastname: Nguyen
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D002243:Carbolines / Q000302:isolation & purification / Q000494:pharmacology*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D035701:Eurycoma / Q000737:chemistry* / Q000166:cytology; D026121:Indole Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000166:cytology; D010946:Plants, Medicinal / Q000737:chemistry / Q000166:cytology; D007769:Lactams
chemical_list: D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002243:Carbolines; D052246:Cyclooxygenase 2 Inhibitors; D026121:Indole Alkaloids; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C000619091:7-methoxy-(9H-beta-carbolin-1-il)-(E)-1-propenoic acid; C574274:9-hydroxycanthin-6-one; C459628:9-methoxycanthin-6-one; D007769:Lactams; C496317:Nos2 protein, mouse
keywords: Eurycoma longifolia; hairy-root cultures; β-carboline alkaloid