title: Enzyme and Mediator-coadsorbed Carbon Felt Electrode for Electrochemical Detection of Glucose Covered with Polymer Layers Based on Layer-by-Layer Technique.
abstract: Glucose dehydrogenase (GlDH) and ferrocene were coadsorbed on a carbon felt (CF) sheet (5 × 10 mm, 2 mm thickness), which was used to construct an electrode for the electrochemical detection of glucose. A potential of +0.3 V vs. Ag/AgCl was applied on the base CF, and the current was measured. After the addition of glucose, the current increased and reached a steady state within 50 s. The current response was proportional to the glucose concentration up to 20 μM, with a lower detection limit of 1 μM. The surface of the CF electrode was covered by layers of polystyrene sulfonate and poly-L-lysine using layer-by-layer technique. Again the current response was proportional to glucose concentration up to 20 μM, with a lower detection limit of 2 μM. The oxidation current owing to electrochemical interferents such as L-ascorbate and acetaminophen was 1/8 times of the current observed on the unprotected electrode. In addition, the protection imparted stability to the electrode. Our work demonstrates that a GlDH/ferrocene CF electrode, protected with polystyrene sulfonate and poly-L-lysine, could be used for the electrochemical detection of glucose.
authors.affiliation: Division of Biomedical Research, National Institute of Advanced Industrial Science and Technology (AIST).
authors.forename: Soichi
authors.initials: S
authors.lastname: Yabuki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hirata
mesh_terms: D000327:Adsorption; D016477:Artifacts; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D011107:Polylysine / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D000077482:Carbon Fiber; D011137:Polystyrenes; D011107:Polylysine; C003321:polystyrene sulfonic acid; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose