title: Non-polar lipids characterization of Quinoa (Chenopodium quinoa) seed by comprehensive two-dimensional gas chromatography with flame ionization/mass spectrometry detection and non-aqueous reversed-phase liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection.
abstract: A chemical characterization of major lipid components, namely, triacylglycerols, fatty acids and the unsaponifiable fraction, in a Quinoa seed lipids sample is reported. To tackle such a task, non-aqueous reversed-phase high-performance liquid chromatography with mass spectrometry detection was employed. The latter was interfaced with atmospheric pressure chemical ionization for the analysis of triacylglycerols. The main triacylglycerols (>10%) were represented by OLP, OOL and OLL (P = palmitoyl, O = oleoyl, L = linoleoyl); the latter was present in the oil sample at the highest percentage (18.1%). Furthermore, fatty acid methyl esters were evaluated by gas chromatography with flame ionization detection. 89% of the total fatty acids was represented by unsaturated fatty acid methyl esters with the greatest percentage represented by linoleic and oleic acids accounting for approximately 48 and 28%, respectively. An extensive characterization of the unsaponifiable fraction of Quinoa seed lipids was performed for the first time, by using comprehensive two-dimensional gas chromatography with dual mass spectrometry/flame ionization detection. Overall, 66 compounds of the unsaponifiable fraction were tentatively identified, many constituents of which (particularly sterols) were confirmed by using gas chromatography with high-resolution time-of-flight mass spectrometry.
authors.affiliation: University Campus Bio-Medico, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Fanali
authors.affiliation: Dipartimento di Scienze del Farmaco e deiProdotti per la Salute, University of Messina, Messina, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Beccaria
authors.affiliation: Dipartimento di Scienze del Farmaco e deiProdotti per la Salute, University of Messina, Messina, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Salivo
authors.affiliation: Dipartimento di Scienze del Farmaco e deiProdotti per la Salute, University of Messina, Messina, Italy.
authors.forename: Peter
authors.initials: P
authors.lastname: Tranchida
authors.affiliation: University Campus Bio-Medico, Rome, Italy.
authors.forename: Giusy
authors.initials: G
authors.lastname: Tripodo
authors.affiliation: Diabetes Research Institute, Division of Cellular Transplantation of Surgery, University of Miami, Miami, FL, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Farnetti
authors.affiliation: University Campus Bio-Medico, Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Dugo
authors.affiliation: University Campus Bio-Medico, Rome, Italy.|Dipartimento di Scienze del Farmaco e deiProdotti per la Salute, University of Messina, Messina, Italy.|Chromaleont S.r.l. A Spin-off of the University of Messina, Messina, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.affiliation: University Campus Bio-Medico, Rome, Italy.|Dipartimento di Scienze del Farmaco e deiProdotti per la Salute, University of Messina, Messina, Italy.|Chromaleont S.r.l. A Spin-off of the University of Messina, Messina, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
keywords: Chenopodium Quinoa; Lipids; Mass spectrometry; Triacylglycerols; Unsaponifiable lipids