title: Immunomodelling Characteristics of Mature Dendritic Cells Stimulated by Colon Cancer Cells Lysates.
abstract: Application of cells with high TAA (tumor associated antigen) presentation potential seems to be crucial in neoplasia immunotherapy. Such feature is distributed in dendritic cells, which present peptides from processed TAA - MHC molecules complex to the T cells of a host. The aim of the study was to assess the influence of colon neoplasia tissue lysate on functioning of generated autologous DC's in the field of autologous CD4+ lymphocytes immunological response towards Th1/Th2 under in vitro environment together with comparison and assessment of DCs' immunosuppressive properties acquired from patients with colon cancer.
  
  
  METHODS
  The population of this study consisted of 16 healthy- controls, 36colon cancer patients. Blood samples were collected 24h before planned surgery and preventive antibiotic therapy. Neoplastic tissue sample, was digested for cell lysates preparation. DC's generation from PBMC was carried out in standard conditionsand medium enriched with rhGM-CSF and rhIL-4. Mature DC`s and cocultured autologous CD4 lymphocytes immunophenotype assessment was analyzed with flow cytometer. Intracellular and culture medium cytokines concentration was analyzed with ELISA and FACS method.
  
  
  RESULTS
  DC`s generated from colon cancer patients stimulated with lysates presented greater maturity, lower expression of CD206 antigen, significantly higher expression of HLA-DR, CD208 and CD209 and high intracellular expression of IL-12, compared to non-stimulated cells.
  
  
  CONCLUSIONS
  The neoplastic tissue in vivo produces a number of substances having an unfavorable effect on immune system, our results suggests using lysates as good dendritic cells stimulators that possibly could have application in colon cancer immunotherapy.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Radej
authors.forename: Jacek
authors.initials: J
authors.lastname: Roliński
authors.forename: Karol
authors.initials: K
authors.lastname: Rawicz-Pruszyński
authors.forename: Paweł
authors.initials: P
authors.lastname: Bury
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Borowski
authors.forename: Jacek
authors.initials: J
authors.lastname: Furmaga
authors.forename: Andrzej
authors.initials: A
authors.lastname: Chrościcki
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wallner
authors.forename: Ryszard
authors.initials: R
authors.lastname: Maciejewski
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging