title: Nucleotide Substitution in 3' Arm of Bovine MIR-2467 in Five Cattle Breeds.
abstract: The T > C single nucleotide polymorphism (SNP) in the MIR2467 gene was investigated in order to confirm its presence in cattle genome and to check for possible differences in its genotype distribution among different breeds. Additional purpose of the study was to investigate in silico potential effect of that substitution on the structure and stability of precursor mir-2467. The study involved 634 individuals of five cattle breeds: Angus, Hereford, Holstein-Friesian, Jersey, and Limousin, which were genotyped using PCR-RFLP assay. In this study, the presence of T > C polymorphism at position 24 was observed in all the cattle breeds excepting Hereford. In addition, the differences in the genotype distribution among analyzed breeds were indicated. On the basis of minimum free energy structure prediction, the C allele was indicated to have possible impact on decreasing the stability of the pre-mir-2467, thus altering its ability to regulate target genes expression.
authors.affiliation: a Laboratory of Molecular Cytogenetics , West Pomeranian University of Technology , Szczecin , Szczecin , Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Łukaszewicz
authors.forename: Szymon
authors.initials: S
authors.lastname: Basiak
authors.forename: Witold Stanisław
authors.initials: WS
authors.lastname: Proskura
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dybus
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D003198:Computer Simulation; D005838:Genotype; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics*; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D020871:RNA Stability / Q000235:genetics; D013816:Thermodynamics
chemical_list: D035683:MicroRNAs
keywords: Cattle; MIR2467; Minimum free energy; Polymorphism