title: Antimicrobial efficacy of combined clarithromycin plus daptomycin against biofilms-formed methicillin-resistant Staphylococcus aureus on titanium medical devices.
abstract: In vitro efficacy of combined eradication therapy with clarithromycin and daptomycin against biofilm-formed methicillin-resistant Staphylococcus aureus on the orthopedic titanium devices was evaluated. The bactericidal effect of this antibiotic was investigated by a re-culture test, the scanning electron microscopy, and fluorescence microscopy using a double-staining dyes. Clarithromycin decreased the amount to half in 24 h. Although MRSA biofilms were not eradicated with clarithromycin or daptomycin alone, clarithromycin combined with daptomycin was useful to sterilize titanium devices within 72 h. This in vitro study showed that combined treatment with clarithromycin plus daptomycin is useful to eradicate staphylococcal biofilms formed on orthopedic devices.
authors.affiliation: Division of Clinical Infectious Diseases & Chemotherapy, Tohoku Pharmaceutical University, Sendai, Japan; Research Division for Development of Anti-infective Agents, Institute of Development Aging and Cancer, Tohoku University, Sendai, Japan. Electronic address: sfuji@tohoku-pharm.ac.jp.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Fujimura
authors.affiliation: Department of Orthopedics, Sendai Orthopedic Hospital, Sendai, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sato
authors.affiliation: Division of Clinical Infectious Diseases & Chemotherapy, Tohoku Pharmaceutical University, Sendai, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hayakawa
authors.affiliation: Division of Clinical Infectious Diseases & Chemotherapy, Tohoku Pharmaceutical University, Sendai, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kawamura
authors.affiliation: Division of Clinical Infectious Diseases & Chemotherapy, Tohoku Pharmaceutical University, Sendai, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Furukawa
authors.affiliation: Research Division for Development of Anti-infective Agents, Institute of Development Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D017291:Clarithromycin / Q000494:pharmacology*; D017576:Daptomycin / Q000494:pharmacology*; D004357:Drug Synergism; D004866:Equipment Contamination / Q000517:prevention & control; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009989:Orthotic Devices; D014025:Titanium
chemical_list: D000900:Anti-Bacterial Agents; D014025:Titanium; D017291:Clarithromycin; D017576:Daptomycin
keywords: Biofilms; Clarithromycin; Combination treatment; Daptomycin; MRSA