title: Optimization of biostimulant for bioremediation of contaminated coastal sediment by response surface methodology (RSM) and evaluation of microbial diversity by pyrosequencing.
abstract: The present study aims to optimize the slow release biostimulant ball (BSB) for bioremediation of contaminated coastal sediment using response surface methodology (RSM). Different bacterial communities were evaluated using a pyrosequencing-based approach in contaminated coastal sediments. The effects of BSB size (1-5cm), distance (1-10cm) and time (1-4months) on changes in chemical oxygen demand (COD) and volatile solid (VS) reduction were determined. Maximum reductions of COD and VS, 89.7% and 78.8%, respectively, were observed at a 3cm ball size, 5.5cm distance and 4months; these values are the optimum conditions for effective treatment of contaminated coastal sediment. Most of the variance in COD and VS (0.9291 and 0.9369, respectively) was explained in our chosen models. BSB is a promising method for COD and VS reduction and enhancement of SRB diversity.
authors.affiliation: Department of Environmental Engineering, Korea Maritime and Ocean University, Busan, South Korea.
authors.forename: Bakthavachallam
authors.initials: B
authors.lastname: Subha
authors.affiliation: Department of Environmental Engineering, Korea Maritime and Ocean University, Busan, South Korea. Electronic address: soyc@kmou.ac.kr.
authors.forename: Young Chae
authors.initials: YC
authors.lastname: Song
authors.affiliation: Nuclear Power Equipment Research Center, Korea Maritime and Ocean University, Busan, South Korea.
authors.forename: Jung Hui
authors.initials: JH
authors.lastname: Woo
mesh_terms: D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019015:Geologic Sediments / Q000382:microbiology*; D059013:Microbial Consortia / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D056910:Republic of Korea
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Biostimulant ball; COD reduction; Coastal sediment; Pyrosequencing; Response surface methodology; Sulfate reducing bacteria