title: Protein profiles in mucosal and systemic compartments in response to Vibrio cholerae in a mouse pulmonary infection model.
abstract: We have recently shown that a mouse lung infection model resulting in acute pneumonia could be used for evaluating the protective immunity induced by mucosal vaccines against Vibrio cholerae. In order to gain insight and better understanding of the pathogenicity of V. cholerae infection, we identified and compared proteins induced by V. cholerae in nasal washes, bronchoalveolar lavages (BAL), and sera. Intranasal administration of V. cholerae increased the concentration of total proteins in nasal washes and BAL fluids, but not in sera. LTQ-Orbitrap hybrid Fourier transform mass spectrometry showed that cytoskeletal proteins, protease inhibitors and anti-inflammatory mediators were present in nasal washes from uninfected mice. The distinctly expressed proteins in nasal washes in response to V. cholerae mainly consisted of protease inhibitors, anti-inflammatory proteins, and anti-microbial proteins. A number of protease inhibitors and anti-inflammatory proteins were selectively expressed in BAL fluids from V. cholerae-infected mice, while cytoskeletal proteins and heat shock proteins were mainly observed in BAL fluids from uninfected mice. A large number of serum complements, protease inhibitors, and acute phase proteins were expressed in V. cholerae-infected mice. Collectively, these results suggest that intranasal administration of V. cholerae leading to acute pneumonia elicited alterations of protein profiles associated with immune homeostasis and host protection in both the mucosal and systemic compartments.
authors.affiliation: Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Seok-Seong
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Baik
authors.affiliation: Clinical Immunology Section, Laboratory Sciences Division, International Vaccine Institute, Seoul 151-919, Republic of Korea.
authors.forename: Jae Seung
authors.initials: JS
authors.lastname: Yang
authors.affiliation: Division of Mass Spectrometry Research, Korea Basic Science Institute, Ochang 863-883, Republic of Korea.
authors.forename: Kun
authors.initials: K
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Cheol-Heui
authors.initials: CH
authors.lastname: Yun
authors.affiliation: Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea. Electronic address: shhan-mi@snu.ac.kr.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Han
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002771:Cholera / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D012141:Respiratory Tract Infections / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D044967:Serum / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D020543:Proteome
keywords: Cholera; Inflammation; Mouse pulmonary infection model; Protein profiling; Vibrio cholerae