title: A novel long non-coding natural antisense RNA is a negative regulator of Nos1 gene expression.
abstract: Long non-coding natural antisense transcripts (NATs) are widespread in eukaryotic species. Although recent studies indicate that long NATs are engaged in the regulation of gene expression, the precise functional roles of the vast majority of them are unknown. Here we report that a long NAT (Mm-antiNos1 RNA) complementary to mRNA encoding the neuronal isoform of nitric oxide synthase (Nos1) is expressed in the mouse brain and is transcribed from the non-template strand of the Nos1 locus. Nos1 produces nitric oxide (NO), a major signaling molecule in the CNS implicated in many important functions including neuronal differentiation and memory formation. We show that the newly discovered NAT negatively regulates Nos1 gene expression. Moreover, our quantitative studies of the temporal expression profiles of Mm-antiNos1 RNA in the mouse brain during embryonic development and postnatal life indicate that it may be involved in the regulation of NO-dependent neurogenesis.
authors.affiliation: Sussex Neuroscience, School of Life Science, University of Sussex, Brighton BN1 9QG, UK.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Korneev
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Maconochie
authors.affiliation: Sussex Neuroscience, School of Life Science, University of Sussex, Brighton BN1 9QG, UK.
authors.forename: Souvik
authors.initials: S
authors.lastname: Naskar
authors.affiliation: Sussex Neuroscience, School of Life Science, University of Sussex, Brighton BN1 9QG, UK.
authors.forename: Elena I
authors.initials: EI
authors.lastname: Korneeva
authors.affiliation: Sussex Neuroscience, School of Life Science, University of Sussex, Brighton BN1 9QG, UK.
authors.forename: Guy P
authors.initials: GP
authors.lastname: Richardson
authors.affiliation: Sussex Neuroscience, School of Life Science, University of Sussex, Brighton BN1 9QG, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: O'Shea
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000235:genetics*; D014176:Protein Biosynthesis; D016372:RNA, Antisense / Q000737:chemistry / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D016372:RNA, Antisense; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D052248:Nitric Oxide Synthase Type I