title: miR-1275: A single microRNA that targets the three IGF2-mRNA-binding proteins hindering tumor growth in hepatocellular carcinoma.
abstract: This study aimed to identify a single miRNA or miR (microRNA) which regulates the three insulin-like growth factor-2-mRNA-binding proteins (IGF2BP1, 2 and 3). Bioinformatics predicted miR-1275 to simultaneously target the three IGF2BPs, and screening revealed miR-1275 to be underexpressed in hepatocellular carcinoma (HCC) tissues. Transfection of HuH-7 cells with miR-1275 suppressed IGF2BPs expression and all three IGF2BPs were confirmed as targets of miR-1275. Ectopic expression of miR-1275 and knockdown of IGF2BPs inhibited malignant cell behaviors, and also reduced IGF1R protein and mRNA. Finally IGF1R was validated as a direct target of miR-1275. These findings indicate that the tumor-suppressor miR-1275 can control HCC tumor growth partially through simultaneously regulating the oncogenic IGF2BPs and IGF1R.
authors.affiliation: The Molecular Pathology Research Group, Department of Pharmacology, German University in Cairo, Main Entrance Al Tagamoa Al Khames, 11835 Cairo, Egypt.
authors.forename: Injie Omar
authors.initials: IO
authors.lastname: Fawzy
authors.affiliation: Department of Clinical Pathology, Ain Shams University, Khalifa El-Maamoun St., Abbasiya Square, 11566 Cairo, Egypt.
authors.forename: Mohammed Tarif
authors.initials: MT
authors.lastname: Hamza
authors.affiliation: Department of Endemic Medicine and Hepatology, Cairo University, Kasr El-Aini St., 11562 Cairo, Egypt.
authors.forename: Karim Adel
authors.initials: KA
authors.lastname: Hosny
authors.affiliation: Department of Endemic Medicine and Hepatology, Cairo University, Kasr El-Aini St., 11562 Cairo, Egypt.
authors.forename: Gamal
authors.initials: G
authors.lastname: Esmat
authors.affiliation: The Molecular Pathology Research Group, Department of Pharmacology, German University in Cairo, Main Entrance Al Tagamoa Al Khames, 11835 Cairo, Egypt.
authors.forename: Hend Mohamed
authors.initials: HM
authors.lastname: El Tayebi
authors.affiliation: The Molecular Pathology Research Group, Department of Pharmacology, German University in Cairo, Main Entrance Al Tagamoa Al Khames, 11835 Cairo, Egypt. Electronic address: ahmed.abdel-aziz@guc.edu.eg.
authors.forename: Ahmed Ihab
authors.initials: AI
authors.lastname: Abdelaziz
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D047368:Tumor Burden / Q000235:genetics; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; C000597236:IGF1R protein, human; C406144:IGF2BP1 protein, human; C498779:IGF2BP2 protein, human; C498780:IGF2BP3 protein, human; C000602308:MIRN1275 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D017451:Receptors, Somatomedin; D017526:Receptor, IGF Type 1
keywords: Hepatocellular carcinoma; Insulin-like growth factor 1 receptor (IGF1R); Insulin-like growth factor-2-mRNA-binding protein (IGF2BP or IMP); Posttranscriptional regulation; miR-1275; miRNA or miR