title: Chicken heat shock protein HSPB1 increases and interacts with αB-crystallin in aged skeletal muscle.
abstract: International trading markets of meat require the animal's age information to prevent cross-contamination of ineligible meat products. Individual livestock age is either evaluated from physiological features or verified by breeding history. However, it remains impossible to perform age verification on meat when a suspicion of error occurred in the importing country. To investigate an age-related protein in skeletal muscle of livestock, we compared protein expression among chicken pectoralis major of different ages. Results indicated that the level of expression of chicken HSPB1, one of the small heat shock proteins, was increased in aged muscles. On the other hand, other heat shock proteins, heat shock factors, and myosin heavy chain isoform did not change the expression levels in aged chicken muscle. In addition, we identified that αB-crystallin interacted with HSPB1 in aged chicken muscle. These results suggest that HSPB1 protein forms complexes with αB-crystallin in aged chicken muscle and suppose to become the candidate of age-related bio-marker for verifying the age of chicken meat.
authors.affiliation: a Department of Agrobioscience, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Ueda
authors.affiliation: a Department of Agrobioscience, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Kokaji
authors.affiliation: a Department of Agrobioscience, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Simizu
authors.affiliation: b Department of Bioresource Science, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Honda
authors.affiliation: c Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University , Kobe , Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yoshino
authors.affiliation: b Department of Bioresource Science, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamisoyama
authors.affiliation: a Department of Agrobioscience, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shirai
authors.affiliation: a Department of Agrobioscience, Graduate School of Agricultural Science , Kobe University , Kobe , Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Yamanoue
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002645:Chickens; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008461:Meat Products / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D038203:alpha-Crystallin B Chain / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D055551:HSP27 Heat-Shock Proteins; D038203:alpha-Crystallin B Chain
keywords: Small heat shock protein; age-related protein; poultry; skeletal muscle; slaughter age