title: Cytoskeletal and mitochondrial properties of bovine oocytes obtained by Ovum Pick-Up: the effects of follicle stimulation and in vitro maturation.
abstract: Follicle stimulation by follicular stimulating hormone (FSH) is known to improve developmental competence of bovine oocytes obtained by Ovum Pick-Up (OPU); however, the exact factors in oocytes affected by this treatment have remained unclear. We compared in vitro matured (IVM) oocytes obtained at the immature stage from cows by OPU either without or with stimulation with FSH (non-stimulated and stimulated OPU, respectively) to those obtained by superstimulation and in vivo maturation in terms of cytoskeleton morphology, mitochondrial distribution, intracellular adenosine triphosphate (ATP) content and H2 O2 levels at the metaphase-II stage and intracellular Ca(2+) levels after in vitro fertilization (IVF). Confocal microscopy after immunostaining revealed reduced size of the meiotic spindle, associated with increased tendencies of microfilament degradation and insufficient mitochondrial re-distribution in non-stimulated OPU-derived IVM oocytes compared with those collected by stimulated OPU, which in turn resembled in vivo matured oocytes. However, there was no difference in mitochondrial functions between oocytes obtained by stimulated or non-stimulated OPU in terms of ATP content, cytoplasmic H2 O2 levels, base Ca(2+) levels and the frequencies and amplitudes of Ca(2+) oscillations after IVF. Larger size of metaphase spindles in oocytes obtained by stimulated OPU may reflect and potentially contribute to their high developmental competence.
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Tamás
authors.initials: T
authors.lastname: Somfai
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Matoba
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Inaba
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Nakai
authors.affiliation: National Livestock Breeding Center, Nishigo, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Imai
authors.affiliation: Food and Fertilizer Technology Center, Taipei, Taiwan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Geshi
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D003599:Cytoskeleton; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D059471:In Vitro Oocyte Maturation Techniques; D008928:Mitochondria; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure*; D006080:Ovarian Follicle
chemical_list: D000255:Adenosine Triphosphate; D005640:Follicle Stimulating Hormone; D002118:Calcium
keywords: bovine oocyte; cytoskeleton; in vitro maturation; in vivo maturation; mitochondria