title: Noise induced reversible changes of cochlear ribbon synapses contribute to temporary hearing loss in mice.
abstract: CONCLUSIONS
  Noise exposure can cause a decline in cochlear ribbon synapses and result in consequent hearing loss. The reduction of synaptic puncta appears reversible and may contribute to hearing restoration in mice after noise exposure.
  
  
  OBJECTIVE
  To detect whether noise induced reversible changes of cochlear ribbon synapses contribute to temporary hearing loss in C57BL/6J mice.
  
  
  METHODS
  The mice were assigned randomly to five groups and exposed to white noise at 110 dB SPL for 2 h except the control group. ABR thresholds were acquired before noise exposure (control), immediately following exposure (Day 0), or on Days 4, 7, or 14 after noise exposure. Light microscopy, scanning emission microscopy, and whole mounts examination was utilized to study whether there is morphology change of outer hair cells (OHC), inner hair cells (IHC), or spiral ganglion cells (SGN) due to the 110 dB white noise. Moreover, experimental approaches, including immunostaining and confocal microcopy, were used to detect whether ribbon synapses were the primary targets of noise-induced temporary hearing loss.
  
  
  RESULTS
  Exposure to 110 dB white noise for 2 h induced TTS in mice, with the maximal ABR threshold elevations seen on the 4(th) day after noise exposure. There were no significant morphological changes in the cochlea. Paralleled changes of pre-synaptic ribbons in both the number and post-synaptic density (PSDs) during this noise exposure were detected. The number of pre-synaptic ribbon, post-synaptic density (PSDs), and co-localized puncta correlated with the shifts of ABR thresholds. Moreover, a complete recovery of ABR thresholds and synaptic puncta was seen on the 14(th) day after the noise stimulations.
authors.affiliation: a 1 Department of Otorhinolaryngology, The First Affiliated Hospital, China Medical University , Shenyang 110001, PR China.|c 3 Department of Otorhinolaryngology, The First Affiliated Hospital, Dalian Medical University , Dalian 116013, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shi
authors.affiliation: b 2 Department of Otolaryngology, Head & Neck Surgery, The Institute of Otolaryngology, Chinese PLA General Hospital , 28 Fuxing Road, Beijing, 100853, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: c 3 Department of Otorhinolaryngology, The First Affiliated Hospital, Dalian Medical University , Dalian 116013, PR China.
authors.forename: Haolin
authors.initials: H
authors.lastname: Wang
authors.affiliation: b 2 Department of Otolaryngology, Head & Neck Surgery, The Institute of Otolaryngology, Chinese PLA General Hospital , 28 Fuxing Road, Beijing, 100853, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: c 3 Department of Otorhinolaryngology, The First Affiliated Hospital, Dalian Medical University , Dalian 116013, PR China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Hong
authors.affiliation: c 3 Department of Otorhinolaryngology, The First Affiliated Hospital, Dalian Medical University , Dalian 116013, PR China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: b 2 Department of Otolaryngology, Head & Neck Surgery, The Institute of Otolaryngology, Chinese PLA General Hospital , 28 Fuxing Road, Beijing, 100853, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: a 1 Department of Otorhinolaryngology, The First Affiliated Hospital, China Medical University , Shenyang 110001, PR China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jiang
authors.affiliation: b 2 Department of Otolaryngology, Head & Neck Surgery, The Institute of Otolaryngology, Chinese PLA General Hospital , 28 Fuxing Road, Beijing, 100853, PR China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Yang
mesh_terms: D000161:Acoustic Stimulation / Q000009:adverse effects; D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D003051:Cochlea / Q000378:metabolism / Q000503:physiopathology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology*; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism / Q000648:ultrastructure*; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism / Q000648:ultrastructure*; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009473:Neuronal Plasticity; D013136:Spiral Ganglion / Q000378:metabolism / Q000648:ultrastructure; D013569:Synapses
keywords: Noise exposure; hearing impairment; inner hair cell; ribbon synapse plasticity