title: Establishment and characterization of SUIT-58 pancreas cancer cell line and its subline S58-SF adapted to serum-free condition derived from metastatic liver tumor.
abstract: A new pancreas cancer cell line, SUIT-58, was established from metastatic liver tumor. The cultured cells exhibited polygonal shape, and proliferated in a form of sheet-structure showing prominent nucleoli and frequent mitotic features. Chromosome count ranged from 54 to 73 with modal chromosome numbers 72 and 73. It was noteworthy that this cell line grew in the serum-free media and maintained in this condition for 30 passages (designated as S58-SF). Both SUIT-58 and S58-SF cell lines were successfully transplanted into nude mice, and their tumor doubling times in xenografts were calculated as 5.4 and 2.8 days, respectively. Histopathologically, the xenografts formed glandular structure that resembled the original tumor. In culture media, the doubling time of SUIT-58 and S58-SF cell lines was calculated as 32 and 35.7 h, respectively. Although the cellular arrangements of SUIT-58 and S58-SF cell lines are different to some extent, their subcellular structures under electron microscope were similar with a large number of lysosomes and distinct desmosomes at cell-cell adhesion sites. The present SUIT-58 and its derivative cell line S58-SF will be applicable for biological studies to develop a new clinical treatment of refractory pancreatic cancer.
authors.affiliation: Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Aoyama
authors.affiliation: Surgical Oncology and Regulation of Organ Function, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Ohuchida
authors.affiliation: Division of Pathophysiology, Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sameshima
authors.affiliation: Division of Pathophysiology, Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Asada
authors.affiliation: Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan. akira_sawaguchi@med.miyazaki-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Sawaguchi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary* / Q000648:ultrastructure; D000368:Aged; D000592:Amino Acid Metabolism, Inborn Errors; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D016895:Culture Media, Serum-Free; D003744:Dental Enamel Hypoplasia; D003920:Diabetes Mellitus; D004392:Dwarfism; D005260:Female; D064593:Heterografts; D006801:Humans; D008607:Intellectual Disability; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000556:secondary* / Q000648:ultrastructure; D008819:Mice, Nude; D008831:Microcephaly; D064347:Microscopy, Electrochemical, Scanning; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016895:Culture Media, Serum-Free
keywords: Cell line; Electron microscopy; Liver metastasis; Pancreas cancer; Protein-free culture