title: [Determination of dimethyl fumarate in bakery food by d-SPE-HPLC-PDA].
abstract: OBJECTIVE
  To establish a simple and rapid pretreatment method with dispersive solid phase extraction ( d-SPE) by HPLC for determination of dimethyl fumarate in bakery foods.
  
  
  METHODS
  Dimethyl fumarate in samples was ultrasonically extracted by methanol, and cleaned up with d-SPE. Then, it was separated on C18 chromatographic column (4.6 mm x 25 mm, 5 μm) with a mixture of methanol--0.03 mol/L sodium acetate and 0.008 mol/L tetrabutyl ammonium bromide (40: 60, V/V) as mobile phase. The photodiode array detector was used in the determination under λ = 220 nm.
  
  
  RESULTS
  In the linear range of 0.1 -25 μg/ml, the correlation coefficients was r > 0.999, and the average recoveries of the spiked samples were in the range of 82.8% - 107.5% with relative standard deviations (RSD) in the range of 3.30% - 7.30% (n = 6). The limit of detection ( LOD) was 0.4 mg/kg, and the limit of quantification was 1.0 mg/kg.
  
  
  CONCLUSIONS
  The method is simple, rapid, sensitive and accurate, and suitable for determine dimethyl fumarate in bakery foods.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Mengtian
authors.initials: M
authors.lastname: Luo
authors.forename: Di
authors.initials: D
authors.lastname: Feng
authors.forename: Hong
authors.initials: H
authors.lastname: Miao
authors.forename: Shufeng
authors.initials: S
authors.lastname: Song
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000069462:Dimethyl Fumarate; D005502:Food; D005506:Food Contamination / Q000032:analysis*; D005650:Fumarates / Q000032:analysis*; D007202:Indicators and Reagents; D057230:Limit of Detection; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D005650:Fumarates; D007202:Indicators and Reagents; D000069462:Dimethyl Fumarate