title: Potential DMSP-degrading Roseobacter clade dominates endosymbiotic microflora of Pyrodinium bahamense var. compressum (Dinophyceae) in vitro.
abstract: Many aspects of the biology and ecology of the toxic dinoflagellate Pyrodinium bahamense var. compressum are still poorly understood. In this brief note, we present identification of its associated intracellular bacteria or endosymbionts via PCR cloning and 16s rRNA gene sequencing and their localization by confocal microscopy, a first for Pyrodinium. The most frequently observed species in the endosymbiotic microflora were from Roseobacter clade (Alphaproteobacteria, 68%) and Gilvibacter sediminis (Flavobacteriaceae, 20%). Roseobacter lineage, the most abundant taxa in this study, is known to be involved in dimethylsulfoniopropionate metabolism which is highly produced in dinoflagellates-a possible strong factor shaping the structure of the associated bacterial community.
authors.affiliation: The Marine Science Institute, University of the Philippines, 1101, Diliman, Quezon City, Philippines.
authors.forename: Deo Florence L
authors.initials: DF
authors.lastname: Onda
authors.forename: Rhodora V
authors.initials: RV
authors.lastname: Azanza
authors.forename: Arturo O
authors.initials: AO
authors.lastname: Lluisma
mesh_terms: D004141:Dinoflagellida / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041904:Roseobacter / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D013452:Sulfonium Compounds / Q000378:metabolism; D013559:Symbiosis
chemical_list: D012336:RNA, Ribosomal, 16S; D013452:Sulfonium Compounds; C068078:dimethylpropiothetin