title: The circadian clock rephases during lateral root organ initiation in Arabidopsis thaliana.
abstract: The endogenous circadian clock enables organisms to adapt their growth and development to environmental changes. Here we describe how the circadian clock is employed to coordinate responses to the key signal auxin during lateral root (LR) emergence. In the model plant, Arabidopsis thaliana, LRs originate from a group of stem cells deep within the root, necessitating that new organs emerge through overlying root tissues. We report that the circadian clock is rephased during LR development. Metabolite and transcript profiling revealed that the circadian clock controls the levels of auxin and auxin-related genes including the auxin response repressor IAA14 and auxin oxidase AtDAO2. Plants lacking or overexpressing core clock components exhibit LR emergence defects. We conclude that the circadian clock acts to gate auxin signalling during LR development to facilitate organ emergence.
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Ute
authors.initials: U
authors.lastname: Voß
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Wilson
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Kim
authors.initials: K
authors.lastname: Kenobi
authors.affiliation: Department of Comparative and Functional Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Gould
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK.
authors.forename: Fiona C
authors.initials: FC
authors.lastname: Robertson
authors.affiliation: Department of Environmental Science &Technology, College of Agriculture &Natural Resources, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Peer
authors.affiliation: 1] Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK [2] Institut de Recherche pour le Développement, UMR DIADE, Montpellier 34394, France.
authors.forename: Mikaël
authors.initials: M
authors.lastname: Lucas
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Kamal
authors.initials: K
authors.lastname: Swarup
authors.affiliation: Departamento Anatomía, Biología Celular Y Zoología, Facultad de Ciencias, Universidad de Extremadura, Badajoz 06006, Spain.
authors.forename: Ilda
authors.initials: I
authors.lastname: Casimiro
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Tara J
authors.initials: TJ
authors.lastname: Holman
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Darren M
authors.initials: DM
authors.lastname: Wells
authors.affiliation: 1] Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK [2] UMR 7265 CEA, CNRS, Université d'Aix-Marseille, Laboratoire de Biologie du Développement des Plantes, Saint-Paul-lez-Durance 13108, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Péret
authors.affiliation: 1] Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK [2] Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Goh
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Fukaki
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: T Charlie
authors.initials: TC
authors.lastname: Hodgman
authors.affiliation: Institut de Recherche pour le Développement, UMR DIADE, Montpellier 34394, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Laplaze
authors.affiliation: 1] School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JH, UK [2] SynthSys, CH Waddington Building, University of Edinburgh, Edinburgh EH9 3JD, UK.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Halliday
authors.affiliation: Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea SE-901 83, Sweden.
authors.forename: Karin
authors.identifier: 000000032901189X
authors.initials: K
authors.lastname: Ljung
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, Maryland 20742, USA.
authors.forename: Angus S
authors.initials: AS
authors.lastname: Murphy
authors.affiliation: Department of Comparative and Functional Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Hall
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK.
authors.forename: Alex A R
authors.initials: AA
authors.lastname: Webb
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Nottingham LE12 5RD, UK.
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
mesh_terms: D017360:Arabidopsis / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D057906:Circadian Clocks / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018522:Gravitropism; D007210:Indoleacetic Acids / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; C507566:IAA14 protein, Arabidopsis; D007210:Indoleacetic Acids; C411822:TOC1 protein, Arabidopsis; D014157:Transcription Factors; C000606697:DAO2 protein, Arabidopsis; D010088:Oxidoreductases