title: Blind source separation of ex-vivo aorta tissue multispectral images.
abstract: Blind Source Separation methods (BSS) aim for the decomposition of a given signal in its main components or source signals. Those techniques have been widely used in the literature for the analysis of biomedical images, in order to extract the main components of an organ or tissue under study. The analysis of skin images for the extraction of melanin and hemoglobin is an example of the use of BSS. This paper presents a proof of concept of the use of source separation of ex-vivo aorta tissue multispectral Images. The images are acquired with an interference filter-based imaging system. The images are processed by means of two algorithms: Independent Components analysis and Non-negative Matrix Factorization. In both cases, it is possible to obtain maps that quantify the concentration of the main chromophores present in aortic tissue. Also, the algorithms allow for spectral absorbance of the main tissue components. Those spectral signatures were compared against the theoretical ones by using correlation coefficients. Those coefficients report values close to 0.9, which is a good estimator of the method's performance. Also, correlation coefficients lead to the identification of the concentration maps according to the evaluated chromophore. The results suggest that Multi/hyper-spectral systems together with image processing techniques is a potential tool for the analysis of cardiovascular tissue.
authors.affiliation: Grupo de Materiales Avanzados y Energía -MatyEr-. Línea Electromedicina. Instituto Tecnológico Metropolitano. Calle 54A No. 30-01 Medellín- Colombia.
authors.forename: July
authors.initials: J
authors.lastname: Galeano
authors.affiliation: Grupo de Dinámica Cardiovascular. Universidad Pontificia Bolivariana, Circular 1 No. 73-76, Medellín- Colombia ; Grupo de Óptica y Espectroscopía -GOE- Universidad Pontificia Bolivariana, Circular 1 No. 73-76, Medellín- Colombia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Perez
authors.affiliation: Grupo de Materiales Avanzados y Energía -MatyEr-. Línea Electromedicina. Instituto Tecnológico Metropolitano. Calle 54A No. 30-01 Medellín- Colombia.
authors.forename: Yonatan
authors.initials: Y
authors.lastname: Montoya
authors.affiliation: Grupo de Materiales Avanzados y Energía -MatyEr-. Línea Electromedicina. Instituto Tecnológico Metropolitano. Calle 54A No. 30-01 Medellín- Colombia.
authors.forename: Deivid
authors.initials: D
authors.lastname: Botina
authors.affiliation: Grupo de Óptica y Espectroscopía -GOE- Universidad Pontificia Bolivariana, Circular 1 No. 73-76, Medellín- Colombia.
authors.forename: Johnson
authors.initials: J
authors.lastname: Garzón
keywords: (000.1430) Biology and medicine; (000.2170) Equipment and techniques; (110.4234) Multispectral and hyperspectral imaging; (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine; (170.6935) Tissue characterization