title: Fabrication of doxorubicin-loaded ellipsoid micelle based on diblock copolymer with a linkage of enzyme-cleavable peptide.
abstract: In this study, a novel micellar drug carrier was fabricated from an amphiphilic diblock copolymer containing poly (ethylene glycol) monomethyl ether (mPEG) and stearic moiety (C18) with a linkage of valine-citrulline (VC), which can be cleaved by cathepsin B (CB) enriched in lysosome of tumor cell. Moreover, the self-assembled micelles were observed as ellipsoid shape with major and minor axis of ca. 169 and 103nm, respectively. Such drug carrier was used to encapsulate anti-cancer drug doxorubicin (DOX), and hence showed a faster drug release behavior in a mimic lysosome condition containing cathepsin B. It was ascribed to the lysosome-sensitivity of the valine-citrulline linkage, which was verified by the size distribution curve shifted to greater size under the same mimic lysosome condition. Furthermore, in comparison with pristine doxorubicin, the encapsulation strategy of as-fabricated micellar carrier resulted in a predominant decrease of cytotoxicity. On the whole, a micellar drug carrier, which can be disassembled by cathepsin B, has been emerging as a potential of specific drug release in lysosome. Additionally, the controlled nanoscale together with elongated structure of such assembled ellipsoid micelles might contribute passive targeting function to tumor tissue and faster cellular uptake behavior (Zhou et al.).
authors.affiliation: Department of Radiology, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
authors.forename: Haitao
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jianqi
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Radiology, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
authors.forename: Jafar
authors.initials: J
authors.lastname: Golzarian
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, BeiBei District, Chongqing 400715, China; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China. Electronic address: huangjin@iccas.ac.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China. Electronic address: jhyu@sist.ecnu.edu.cn.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Yu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D000903:Antibiotics, Antineoplastic; D004798:Enzymes; D008823:Micelles; D010455:Peptides; D011108:Polymers; D004317:Doxorubicin
keywords: Cytotoxicity; Lysosome-sensitive peptide; Micellar drug carrier; Self-assembly; Valine-citrulline linkage