title: Dynamic Expression Profiles of Marker Genes in Osteogenic Differentiation of Human Bone Marrow-derived Mesenchymal Stem Cells.
abstract: OBJECTIVE
  To observe the expression profiles of osteoblast-related genes in human mesenchymal stem cells (MSCs) derived from bone marrow during osteogenic differentiation.
  
  
  METHODS
  MSCs were induced to differentiate with MSC osteogenic differentiation medium for 7, 14, 21 and 28 days respectively. Alizarin Red staining was used to detect matrix mineralization. Expression of osteoblast-related genes, including osteocalcin, osteopontin, Runt-related transcription factor 2 (Runx2), alkaline phosphatase and collagen type 1, was assessed with quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  On day 14 after induction of differentiation, cells were stained positively with Alizarin Red. The expression levels of these genes exhibited an upward trend as induction time was prolonged. Exposure to osteogenic differentiation medium less than 21 days did not significantly induce osteocalcin expression; osteocalcin expression levels in the differentiated cells induced for 21 and 28 days were 1.63 and 2.46 times as high as the undifferentiated cells respectively (all P<0.05). Stimulation with MSC osteogenic differentiation medium over 14 days significantly enhanced bone marrow-derived MSCs to express osteopontin and Runx2 genes (all P<0.05). Osteogenic differentiation medium could significantly induce the expressions of alkaline phosphatase and collagen type1 genes (all P<0.05). Their expressions reached the peak levels on day 21, which were increased more than 4- and 3-fold respectively.
  
  
  CONCLUSIONS
  Human bone marrow-derived MSCs could exhibit the sequential expression pattern of osteoblast marker genes during osteogenic differentiation in vitro.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Zheng-yao
authors.initials: ZY
authors.lastname: Li
authors.forename: Yi-peng
authors.initials: YP
authors.lastname: Wang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics; D010012:Osteogenesis; D059467:Transcriptome
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D005819:Genetic Markers; C493709:RUNX2 protein, human; D015675:Osteocalcin; D000469:Alkaline Phosphatase