title: Increased expression of the NLRP3 inflammasome components in patients with Behçet's disease.
abstract: BACKGROUND
  Behçet's disease (BD) is a systemic inflammatory disease with manifestations including recurrent oral and genital ulcerations, and vasculitis involving the skin, mucosa, joints, eyes, veins, arteries, nervous and gastrointestinal systems. BD is seen as a disease at the crossroad between autoimmune and autoinflammatory syndromes, possibly triggered by an aberrant response to infectious stimuli. The relevance of Gram negative bacteria-mediated oral inflammation with the increased expression of NACHT, LRR, and PYD domains-containing protein 3 (NLRP3), leading to systemic inflammation, prompted us to investigate the expression of NLRP3 inflammasome components and its link with IL-1β hypersecretion.
  
  
  RESULTS
  When peripheral blood mononuclear cells (PBMCs) from 15 active, 15 stable BD patients and 15 healthy volunteers were stimulated, the basal and LPS-induced expressions of NLRP3 inflammasome components were significantly increased at both mRNA and protein levels in BD patients compared to healthy controls. Also, increased expression of NLRP3 and ASC was observed in 25 BD skin lesions compared to 25 erythema nodosum patients. Compatible with this, secretion of IL-1β by PBMCs stimulated with LPS alone or LPS plus ATP was increased in BD compared to healthy controls, which was suppressed by caspase-1 inhibitor.
  
  
  CONCLUSIONS
  Our findings suggest the possible link between increased IL-1β secretion and increased expression of NLRP3 inflammasome components in BD patients with skin manifestations.
authors.affiliation: Department of Dermatology, Ajou University School of Medicine, 164 Worldcup-ro, Yeongtong-Gu, Suwon, 443-380 South Korea ; Present address: Department of Dermatology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: En Hyung
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Ajou University School of Medicine, 164 Worldcup-ro, Yeongtong-Gu, Suwon, 443-380 South Korea.
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Department of Biomedical Sciences, The Graduate School, Ajou University and Department of Microbiology, Ajou University School of Medicine, Suwon, South Korea.
authors.forename: Sun
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Dermatology, Ajou University School of Medicine, 164 Worldcup-ro, Yeongtong-Gu, Suwon, 443-380 South Korea.
authors.forename: Eun-So
authors.initials: ES
authors.lastname: Lee
keywords: Behçet’s disease; Inflammasome; Interleukin-1β; NLRP3