title: Investigating the Role of the Posttranscriptional  Gene Regulator MiR-24-  3p in the Proliferation, Migration and  Apoptosis of Human Arterial Smooth  Muscle Cells in Arteriosclerosis Obliterans.
abstract: OBJECTIVE
  To explore the expression of miR-24-3p in human arteries with arteriosclerosis obliterans (ASO) as well as the role of miR-24-3p in the pathogenesis of ASO.
  
  
  METHODS
  We used quantitative real-time PCR (qRT-PCR) and in situ hybridization to monitor miR-24-3p expression in human arteries. To investigate the effect of miR-24-3p on human arterial smooth muscle cells (HASMCs), we applied cell counting and EdU assays to monitor proliferation and transwell and wound healing assays to investigate migration and flow cytometry to investigate apoptosis. Furthermore, we applied 3'-untranslated region (3'-UTR) luciferase assays to investigate the role of miR-24-3p in targeting platelet-derived growth factor receptor B (PDGFRB) and c-Myc.
  
  
  RESULTS
  MiR-24-3p was mainly located in the media of arteries and was downregulated in ASO arteries compared with normal arteries. Platelet-derived growth factor BB (PDGF-BB) treatment reduced the expression of miR-24-3p in primary cultured HASMCs. MiR-24-3p mimic oligos inhibited the proliferation and migration, and promotes apoptosis of HASMCs. Our 3'-UTR luciferase assays confirmed that PDGFRB and c-Myc were targets of miR-24-3p.
  
  
  CONCLUSIONS
  The results suggest that miR-24-3p regulates the proliferation and migration of HASMCs by targeting PDGFRB and c-Myc. The PDGF/miR-24-3p/PDGFRB and PDGF/miR-24-3p/c-Myc pathways may play critical roles in the pathogenesis of ASO. These findings highlight the potential for new therapeutic targets for ASO.
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Zhu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shan
authors.forename: Jie-yi
authors.initials: JY
authors.lastname: Ma
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.forename: Chun-xiang
authors.initials: CX
authors.lastname: Zhang
authors.forename: Rui-ming
authors.initials: RM
authors.lastname: Liu
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Wu
authors.forename: Ya-wei
authors.initials: YW
authors.lastname: Shi
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Shen-ming
authors.initials: SM
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D001162:Arteriosclerosis Obliterans / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics
chemical_list: C543845:MIRN24 microRNA, human; C489427:MYC protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D020797:Receptor, Platelet-Derived Growth Factor beta