title: Interleukin 35 (IL-35) and IL-37: Intestinal and peripheral expression by T and B regulatory cells in patients with Inflammatory Bowel Disease.
abstract: The aim of the study was to characterize and to quantify peripheral and tissue. IL-35- and IL-37-producing cells in Inflammatory Bowel Disease (IBD) patients. We studied a total of 38 active UC, 31 inactive UC, 17 active CD, and 13 inactive CD and 50 non-inflamed tissues as control group. Gene expression was measured by real time polymerase chain reaction (RT-PCR) and protein expression was evaluated in tissue by immunohistochemistry and in peripheral blood mononuclear cells by flow cytometry. Higher levels of IL-35 was produced by intestinal regulatory B cells and circulating regulatory CD4(+) and CD8(+) T cells in active vs. inactive disease or healthy donors (P<0.05). The IL-37 was conspicuously synthesized by circulating B cells, active natural killer cells and monocytes. These results suggest that down-regulation of inflammation in active IBD patients might be based on the increased expression of IL-35 and IL-37.
authors.affiliation: Inflammatory Bowel Disease Clinic, Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, Vasco de Quiroga No. 15, Col. Sección XVI, CP 14000 Mexico City, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Fonseca-Camarillo
authors.affiliation: Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, Vasco de Quiroga No. 15, Col. Sección XVI, CP 14000 Mexico City, Mexico.
authors.forename: Janette
authors.initials: J
authors.lastname: Furuzawa-Carballeda
authors.affiliation: Inflammatory Bowel Disease Clinic, Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, Vasco de Quiroga No. 15, Col. Sección XVI, CP 14000 Mexico City, Mexico. Electronic address: kazuofurusho@hotmail.com.
authors.forename: Jesús K
authors.initials: JK
authors.lastname: Yamamoto-Furusho
mesh_terms: D000328:Adult; D000368:Aged; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003093:Colitis, Ulcerative / Q000276:immunology* / Q000473:pathology; D003424:Crohn Disease / Q000276:immunology* / Q000473:pathology; D003430:Cross-Sectional Studies; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000096:biosynthesis; D007249:Inflammation / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology*; D016753:Interleukin-10 / Q000096:biosynthesis; D053650:Interleukin-3 Receptor alpha Subunit / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; C508587:IL37 protein, human; C115022:IL3RA protein, human; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007375:Interleukin-1; D053650:Interleukin-3 Receptor alpha Subunit; D007378:Interleukins; C532417:interleukin-35, human; D016753:Interleukin-10
keywords: Crohn’s disease; IDO; IL-37; Interleukin (IL)-35; Ulcerative colitis