title: Micelle-Encapsulated Quantum Dot-Porphyrin Assemblies as in Vivo Two-Photon Oxygen Sensors.
abstract: Micelles have been employed to encapsulate the supramolecular assembly of quantum dots with palladium(II) porphyrins for the quantification of O2 levels in aqueous media and in vivo. Förster resonance energy transfer from the quantum dot (QD) to the palladium porphyrin provides a means for signal transduction under both one- and two-photon excitation. The palladium porphyrins are sensitive to O2 concentrations in the range of 0-160 Torr. The micelle-encapsulated QD-porphyrin assemblies have been employed for in vivo multiphoton imaging and lifetime-based oxygen measurements in mice with chronic dorsal skinfold chambers or cranial windows. Our results establish the utility of the QD-micelle approach for in vivo biological sensing applications.
authors.affiliation: †Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.|‡Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Lemon
authors.affiliation: ‡Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Karnas
authors.affiliation: §Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Cox-7, Boston, Massachusetts 02114, United States.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Han
authors.affiliation: ‡Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Oliver T
authors.initials: OT
authors.lastname: Bruns
authors.affiliation: †Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Kempa
authors.affiliation: §Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Cox-7, Boston, Massachusetts 02114, United States.
authors.forename: Dai
authors.initials: D
authors.lastname: Fukumura
authors.affiliation: ‡Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Moungi G
authors.initials: MG
authors.lastname: Bawendi
authors.affiliation: §Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Cox-7, Boston, Massachusetts 02114, United States.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
authors.affiliation: §Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Cox-7, Boston, Massachusetts 02114, United States.
authors.forename: Dan G
authors.initials: DG
authors.lastname: Duda
authors.affiliation: †Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Nocera
mesh_terms: D000818:Animals; D002214:Capsules; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D008665:Metalloporphyrins / Q000737:chemistry*; D051379:Mice; D008823:Micelles; D008958:Models, Molecular; D008968:Molecular Conformation; D057054:Molecular Imaging; D010100:Oxygen / Q000032:analysis*; D010165:Palladium / Q000737:chemistry; D017785:Photons; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D002214:Capsules; D008665:Metalloporphyrins; D008823:Micelles; D010165:Palladium; D010100:Oxygen