title: Intracellular dynamics and fate of polystyrene nanoparticles in A549 Lung epithelial cells monitored by image (cross-) correlation spectroscopy and single particle tracking.
abstract: Novel insights in nanoparticle (NP) uptake routes of cells, their intracellular trafficking and subcellular targeting can be obtained through the investigation of their temporal and spatial behavior. In this work, we present the application of image (cross-) correlation spectroscopy (IC(C)S) and single particle tracking (SPT) to monitor the intracellular dynamics of polystyrene (PS) NPs in the human lung carcinoma A549 cell line. The ensemble kinetic behavior of NPs inside the cell was characterized by temporal and spatiotemporal image correlation spectroscopy (TICS and STICS). Moreover, a more direct interpretation of the diffusion and flow detected in the NP motion was obtained by SPT by monitoring individual NPs. Both techniques demonstrate that the PS NP transport in A549 cells is mainly dependent on microtubule-assisted transport. By applying spatiotemporal image cross-correlation spectroscopy (STICCS), the correlated motions of NPs with the early endosomes, late endosomes and lysosomes are identified. PS NPs were equally distributed among the endolysosomal compartment during the time interval of the experiments. The cotransport of the NPs with the lysosomes is significantly larger compared to the other cell organelles. In the present study we show that the complementarity of ICS-based techniques and SPT enables a consistent elaborate model of the complex behavior of NPs inside biological systems.
authors.affiliation: Biomedical Research Unit, Hasselt University, Agoralaan Building C, 3590 Diepenbeek, Belgium; Flemish Institute For Technological Research, Environmental Risk and Health Unit, Boeretang 200, 2400 Mol, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Deville
authors.affiliation: Biomedical Research Unit, Hasselt University, Agoralaan Building C, 3590 Diepenbeek, Belgium; Department of Radiation Oncology, School of Medicine, University of Pennsylvania, 3400 Civic Boulevard, Philadelphia, PA 19104, USA.
authors.forename: Rozhin
authors.initials: R
authors.lastname: Penjweini
authors.affiliation: Biomedical Research Unit, Hasselt University, Agoralaan Building C, 3590 Diepenbeek, Belgium; Flemish Institute For Technological Research, Environmental Risk and Health Unit, Boeretang 200, 2400 Mol, Belgium.
authors.forename: Nick
authors.initials: N
authors.lastname: Smisdom
authors.affiliation: Biomedical Research Unit, Hasselt University, Agoralaan Building C, 3590 Diepenbeek, Belgium.
authors.forename: Kristof
authors.initials: K
authors.lastname: Notelaers
authors.affiliation: Flemish Institute For Technological Research, Environmental Risk and Health Unit, Boeretang 200, 2400 Mol, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Nelissen
authors.affiliation: Flemish Institute For Technological Research, Environmental Risk and Health Unit, Boeretang 200, 2400 Mol, Belgium; Theoretical Physics, Hasselt University, Agoralaan Building D, 3590 Diepenbeek, Belgium.
authors.forename: Jef
authors.initials: J
authors.lastname: Hooyberghs
authors.affiliation: Biomedical Research Unit, Hasselt University, Agoralaan Building C, 3590 Diepenbeek, Belgium. Electronic address: marcel.ameloot@uhasselt.be.
authors.forename: Marcel
authors.initials: M
authors.lastname: Ameloot
mesh_terms: D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D053758:Nanoparticles; D011137:Polystyrenes; D013057:Spectrum Analysis
chemical_list: D011137:Polystyrenes
keywords: Colocalization; Diffusion; Image correlation spectroscopy; Polystyrene nanoparticles; Single particle tracking