title: EphB1 and EphB2 intracellular domains regulate the formation of the corpus callosum and anterior commissure.
abstract: The two cortical hemispheres of the mammalian forebrain are interconnected by major white matter tracts, including the corpus callosum (CC) and the posterior branch of the anterior commissure (ACp), that bridge the telencephalic midline. We show here that the intracellular signaling domains of the EphB1 and EphB2 receptors are critical for formation of both the ACp and CC. We observe partial and complete agenesis of the corpus callosum, as well as highly penetrant ACp misprojection phenotypes in truncated EphB1/2 mice that lack intracellular signaling domains. Consistent with the roles for these receptors in formation of the CC and ACp, we detect expression of these receptors in multiple brain regions associated with the formation of these forebrain structures. Taken together, our findings suggest that a combination of forward and reverse EphB1/2 receptor-mediated signaling contribute to ACp and CC axon guidance.
authors.affiliation: Department of Psychiatry, Harvard Medical School, McLean Hospital, Belmont, Massachusetts, 02478.|Department of Psychiatry, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Robichaux
authors.affiliation: Department of Psychiatry, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390.|Developmental Biology, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390.
authors.forename: George
authors.initials: G
authors.lastname: Chenaux
authors.affiliation: Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, 02115.
authors.forename: Hsin-Yi Henry
authors.initials: HY
authors.lastname: Ho
authors.affiliation: Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, 02115.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Soskis
authors.affiliation: Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, 02115.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Greenberg
authors.affiliation: Developmental Biology, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390.
authors.forename: Mark
authors.initials: M
authors.lastname: Henkemeyer
authors.affiliation: Department of Psychiatry, Harvard Medical School, McLean Hospital, Belmont, Massachusetts, 02478.|Department of Psychiatry, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Cowan
mesh_terms: D000818:Animals; D066240:Anterior Commissure, Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D001369:Axons / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D003337:Corpus Callosum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D055879:Gene Knock-In Techniques; D055786:Gene Knockout Techniques; D007150:Immunohistochemistry; D042541:Intracellular Space; D008822:Mice, Transgenic; D056347:Neuroanatomical Tract-Tracing Techniques; D000072417:Protein Domains; D036225:Receptor, EphB1 / Q000235:genetics / Q000378:metabolism*; D036183:Receptor, EphB2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C586311:Ephb2 protein, mouse; D036225:Receptor, EphB1; D036183:Receptor, EphB2
keywords: EphB; anterior commissure; axon guidance; corpus callosum; ephrin