title: A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis.
abstract: We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.
authors.affiliation: Department of Chemistry, Research Institute for Natural Sciences, Korea University, Seoul 136-701, South Korea. sw_lee@korea.ac.kr.
authors.forename: Hangyeore
authors.initials: H
authors.lastname: Lee
authors.forename: Dong-Gi
authors.initials: DG
authors.lastname: Mun
authors.forename: Jingi
authors.initials: J
authors.lastname: Bae
authors.forename: Hokeun
authors.initials: H
authors.lastname: Kim
authors.forename: Se Yeon
authors.initials: SY
authors.lastname: Oh
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Park
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Lee
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
mesh_terms: D001331:Automation; D002851:Chromatography, High Pressure Liquid; D040901:Proteomics / Q000295:instrumentation*