title: Involvement of microRNA214 and transcriptional regulation in reductions in mevalonate pyrophosphate decarboxylase mRNA levels in stroke-prone spontaneously hypertensive rat livers.
abstract: Hypocholesterolemia has been epidemiologically identified as one of the causes of stroke (cerebral hemorrhage). We previously reported that lower protein levels of mevalonate pyrophosphate decarboxylase (MPD), which is responsible for reducing serum cholesterol levels in stroke-prone spontaneously hypertensive rats (SHRSP), in the liver were caused by a reduction in mRNA levels. However, the mechanism responsible for reducing MPD expression levels in the SHRSP liver remains unclear. Thus, we compared microRNA (miR)-214 combined with the 3'-untranslated region of MPD mRNA and heterogeneous nuclear RNA (hnRNA) between SHRSP and normotensive Wistar Kyoto rats (WKY). miR-214 levels in the liver were markedly higher in SHRSP than in WKY, whereas hnRNA levels were significantly lower. These results indicate that the upregulation of miR-214 and downregulation of MPD transcription in the liver both play a role in the development of hypocholesterolemia in SHRSP.
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Michihara
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Norie
authors.initials: N
authors.lastname: Ide
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Yurika
authors.initials: Y
authors.lastname: Mizutani
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Maya
authors.initials: M
authors.lastname: Uchida
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuoka
authors.affiliation: a Faculty of Pharmacy & Pharmaceutical Sciences , Fukuyama University , Fukuyama , Hiroshima , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Akasaki
mesh_terms: D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002784:Cholesterol / Q000172:deficiency* / Q000378:metabolism; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D020521:Stroke / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D014158:Transcription, Genetic
chemical_list: D035683:MicroRNAs; C559947:Mirn214 microRNA, rat; D012333:RNA, Messenger; D002784:Cholesterol; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase
keywords: liver; mevalonate pyrophosphate decarboxylase; microRNA; rat; stroke