title: Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity.
abstract: In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanno
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Kumi Y
authors.initials: KY
authors.lastname: Inoue
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suda
authors.forename: Ryota
authors.initials: R
authors.lastname: Kunikata
authors.forename: Masahki
authors.initials: M
authors.lastname: Matsudaira
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002449:Cell Aggregation; D004563:Electrochemistry / Q000295:instrumentation*; D058732:Embryoid Bodies / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D010084:Oxidation-Reduction; D018511:Systems Integration
chemical_list: D000469:Alkaline Phosphatase