title: Investigating the Potential of Amnion-Based Scaffolds as a Barrier Membrane for Guided Bone Regeneration.
abstract: Guided bone regeneration is a new concept of large bone defect therapy, which employs a barrier membrane to afford a protected room for osteogenesis and prevent the invasion of fibroblasts. In this study, we developed a novel barrier membrane made from lyophilized multilayered acellular human amnion membranes (AHAM). After decellularization, the AHAM preserved the structural and biomechanical integrity of the amnion extracellular matrix (ECM). The AHAM also showed minimal toxic effects when cocultured with mesenchymal stem cells (MSCs), as evidenced by high cell density, good cell viability, and efficient osteogenic differentiation after 21-day culturing. The effectiveness of the multilayered AHAM in guiding bone regeneration was evaluated using an in vivo rat tibia defect model. After 6 weeks of surgery, the multilayered AHAM showed great efficiency in acting as a shield to avoid the invasion of the fibrous tissues, stabilizing the bone grafts and inducing the massive bone growth. We hence concluded that the advantages of the lyophilized multilayered AHAM barrier membrane are as follows: preservation of the structural and mechanical properties of the amnion ECM, easiness for preparation and handling, flexibility in adjusting the thickness and mechanical properties to suit the application, and efficiency in inducing bone growth and avoiding fibrous tissues invasion.
authors.affiliation: †Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Liaoning 116001, China.
authors.forename: Wuwei
authors.initials: W
authors.lastname: Li
authors.affiliation: †Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Liaoning 116001, China.
authors.forename: Guowu
authors.initials: G
authors.lastname: Ma
authors.affiliation: ‡Department of Biological Engineering and College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Bryn
authors.initials: B
authors.lastname: Brazile
authors.affiliation: †Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Liaoning 116001, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: §Department of Operation Room, The First Affiliated Hospital, Dalian Medical University, Liaoning 116001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
authors.affiliation: ‡Department of Biological Engineering and College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: J Ryan
authors.initials: JR
authors.lastname: Butler
authors.affiliation: ‡Department of Biological Engineering and College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Claude
authors.affiliation: ∥Comprehensive Transplant Center and Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, United States.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Wertheim
authors.affiliation: ‡Department of Biological Engineering and College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Jun
authors.initials: J
authors.lastname: Liao
authors.affiliation: †Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Liaoning 116001, China.|∥Comprehensive Transplant Center and Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
mesh_terms: D000650:Amnion / Q000378:metabolism*; D000818:Animals; D001861:Bone Regeneration; D002470:Cell Survival; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D048091:Guided Tissue Regeneration; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*