title: MiR-1271 Inhibits Cell Proliferation,  Invasion and EMT in Gastric Cancer by  Targeting FOXQ1.
abstract: OBJECTIVE
  FOXQ1 overexpression has been reported to enhance tumor growth and invasion. However, the biological function of FOXQ1 and the mechanism underlying its upregulation in gastric cancer (GC) remain unknown.
  
  
  METHODS
  QPCR was used to detect the expression of miR-1271 and FOXQ1 in specimens from GC patients. FOXQ1-siRNA, and miR- 1271 mimics and inhibitor were transfected into human MGC-803 and SGC-7901 cells. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter assay. Markers of epithelial-mesenchymal transition (EMT) were detected by western blot analysis.
  
  
  RESULTS
  MiR-1271 was downregulated in both GC tissues and GC cell lines. The expression of miR-1271 was inversely correlated with tumor size (P = 0.017), tumor stage (P = 0.035), lymph node metastasis (P = 0.018), and TNM stage (P = 0.025). Ectopic expression of miR-1271 dramatically suppressed GC cell proliferation, invasion, and EMT. Furthermore, FOXQ1 was identified as a direct target of miR-1271. Knockdown of FOXQ1 inhibited GC cell malignant behavior, whereas FOXQ1 overexpression partially restored the suppression effects of miR-1271. Additionally, miR-1271 expression was negatively correlated with FOXQ1 in GC tissues.
  
  
  CONCLUSIONS
  MiR-1271 inhibits cell proliferation, invasion, and EMT in GC by directly suppressing FOXQ1 expression.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Xiang
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Liu
authors.forename: Lu-Ying
authors.initials: LY
authors.lastname: Wan
authors.forename: Miao
authors.initials: M
authors.lastname: Feng
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xiong
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C484579:FOXQ1 protein, human; D051858:Forkhead Transcription Factors; C556309:MIRN1271 microRNA, human; D035683:MicroRNAs