title: Tissue performance of bladder following stretched electrospun silk fibroin matrix and bladder acellular matrix implantation in a rabbit model.
abstract: The goal of this study was to investigate the tissue performance of bladder following stretched electrospun silk fibroin matrix (SESFM) implantation compared with bladder acellular matrix (BAM). We compared SESFM with BAM based on porosity and pore size. Scaffolds were separately transplanted into opposite walls of the bladder of 30 rabbits after stripping the bladder mucosa and smooth muscle (1.5 × 2.0 cm(2)). Gross anatomical observation, histological analysis and muscle contractility studies were performed at 2, 4, and 8 weeks post-op. SESFM has higher porosity and larger pore size compared with BAM (p < 0.05). At 2 weeks, the presence of vesical calculus was evident in 7/10 rabbits. Histological analysis showed that SESFM and BAM promoted similar degree of urothelium regeneration (p > 0.05). However, SESFM promoted a higher degree of smooth muscle and vessel regeneration compared to BAM (p < 0.05). In addition, muscle strips supported by SESFM displayed higher contractile responses to carbachol, KCl, and phenylephrine compared with BAM. At 8 weeks, both matrices elicited similar mild acute and chronic inflammatory reactions. Our results demonstrated that SESFM has greater ability to promote bladder tissue regeneration with structural and functional properties compared to BAM, and with similar biocompatibility.
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Huang
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China.
authors.forename: Zhao-Bo
authors.initials: ZB
authors.lastname: Li
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157.
authors.forename: Sean V
authors.initials: SV
authors.lastname: Murphy
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China.
authors.forename: Qiang-Qiang
authors.initials: QQ
authors.lastname: Liu
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhu
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China.
authors.forename: Yao-Peng
authors.initials: YP
authors.lastname: Zhang
authors.affiliation: Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
authors.forename: Lu-Jie
authors.initials: LJ
authors.lastname: Song
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005349:Fibroins / Q000494:pharmacology*; D007150:Immunohistochemistry; D023421:Models, Animal; D009119:Muscle Contraction / Q000187:drug effects; D016062:Porosity; D019919:Prosthesis Implantation; D011817:Rabbits; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
chemical_list: D005349:Fibroins
keywords: bladder acellular matrix; bladder reconstruction; electrospinning; silk fibroin; stretching