title: Effects of a Terrified-Sound Stress on Serum Proteomic Profiling in Mice.
abstract: The serum proteomic profiles of mice exposed to terrified-sound-induced stress and after stress release were investigated. Serum samples from 32 mice were divided into four groups (n = 8 each) and analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry techniques (MALDI-TOF MS) combined with magnetic bead-based weak cation-exchange chromatography. ClinProTools software identified several distinct markers that differed between the stressed and control groups and between the stress released and stressed released controls. Of 33 m/z peaks that differed among the four groups, 17 were significantly different (P < 0.05). Five peaks (m/z: 2793.37, 2924.86, 1979.90, 3492.49, 3880.24) showed significant differences in expression after exposure to terrified-sound stress and returned to control levels after stress release. These were sequence identified as peptide regions of dimethylaniline monooxygenase, myosin-9, uncharacterized protein in Rattus norvegicus, apolipoprotein C-I, and plasma serine protease inhibitor (Serpina 5). Our study provides the first evidence of significant changes in serum proteomic profiles in mice exposed to terrified-sound stress, which suggests that protein expression profiles are affected by the stress. Normal expression levels were restored after stress release, suggesting the activation of self-adjustment mechanisms for the recovery of protein expression levels altered by this stress.
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.|Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Assisted Reproduction Center, Maternal & Child Health Care Hospital, Xi'an, Shaanxi, 710003, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Liying
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China.
authors.forename: Tusheng
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Cell Biology and Genetics, Basic Medical School, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China. hchen@mail.xjtu.edu.cn.|Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, Xi'an Jiaotong University, 76 Western Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China. hchen@mail.xjtu.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D053302:Apolipoprotein C-I / Q000097:blood; D005239:Fear; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009218:Myosins / Q000097:blood; D010105:Oxygenases / Q000097:blood; D018046:Protein C Inhibitor / Q000097:blood; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D013016:Sound / Q000009:adverse effects*; D013315:Stress, Psychological / Q000097:blood* / Q000209:etiology
chemical_list: D053302:Apolipoprotein C-I; D018046:Protein C Inhibitor; D020543:Proteome; C513537:SERPINA5 protein, mouse; C113469:myosin IXB; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming); D009218:Myosins
keywords: Protein expression; Serum proteomic profiling; Stress release; Terrified-sound-induced stress