title: Decrease of miR-146a is associated with the aggressiveness of human oral squamous cell carcinoma.
abstract: With the aim to identify microRNAs that may contribute to oral squamous cell carcinoma (OSCC) progression, we compared the microRNA expression profiles of two related cell lines that form tumors with differential aggressiveness. A panel of 28 microRNAs was found to be more than 1.5-fold altered, among which miR-146a was the most significantly changed (-4.6-fold). Loss of miR-146a expression was validated in human high-grade tumors, while normal oral mucosa retained expression, using fluorescence in situ hybridization on a tissue microarray. Restoration of miR-146a in SCC25 and UMSCC1 cells decreased in vitro invasive activity, suppressed tumor growth in vivo, and decreased the incidence of UMSCC1 lung metastasis. The transcription factor Sox2 was found to be a putative target of miR-146a. In conclusion, the loss or decrease of miR-146a is a new feature that is associated with more aggressive behaviour in oral squamous carcinoma.
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, United States; Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, United States. Electronic address: zshi2@nd.edu.
authors.forename: Zonggao
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, United States; Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, United States.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Johnson
authors.affiliation: Emory University, Atlanta, GA 30322, United States.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, United States; Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, United States.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, United States; Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, United States.
authors.forename: M Sharon
authors.initials: MS
authors.lastname: Stack
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D064593:Heterografts; D006801:Humans; D066298:In Vitro Techniques; D008156:Luciferases; D008163:Luminescent Measurements; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000473:pathology*; D016133:Polymerase Chain Reaction
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D008156:Luciferases
keywords: Metastasis; MicroRNA; Oral cancer; miR-146a