title: Development and clinical evaluation of a new gold-immunochromatographic assay for the detection of antibodies against field strains of pseudorabies virus.
abstract: An immunochromatographic strip (ICS) was developed for the detection of swine antibodies against glycoprotein E (gE) in Pseudorabies Virus (PRV). In this test, Staphylococcal Protein A (SPA) labeled with colloidal gold was dispensed on a conjugate pad as the detector. Purified PRV-gE and pig-IgG were blotted on a nitrocellulose membrane for the test (T) and control lines (C), respectively. If the tested serum contains IgG antibodies against PRV-gE, the IgG will interact with the colloidal gold-SPA to form a complex (gold-SPA-swine IgG). The complex will react with the immobilized PRV-gE on the T line and the Pig-IgG in the C line of the ICS to form two visible red bands. If there is no IgG antibody against PRV-gE in the sample serum, only the C line will be visible. The ICS was capable of specifically detecting PRV-gE antibody within 5 min, and its stability and reproducibility were quite good after storage at 4°C and use within 4 months. Using an IDEXX Pseudorabies Virus gE Antibody Test Kit (IDEXX PRV gE Ab test) as a reference, the relative specificity and sensitivity of the ICS were determined to be 81.6% and 90.7%, respectively. Furthermore, there was a good agreement between the results obtained by the commercial product and the ICS (kappa=0.7289).
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Dian-lei
authors.initials: DL
authors.lastname: Guo
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Pan
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Kun-peng
authors.initials: KP
authors.lastname: Li
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Jun-qing
authors.initials: JQ
authors.lastname: Li
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Han-wei
authors.initials: HW
authors.lastname: Shen
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Xiang-ling
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Xun-yun
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Xue-song
authors.initials: XS
authors.lastname: Li
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fu
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Nangang District, Harbin 150001, China. Electronic address: lx2005@126.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002846:Chromatography, Affinity / Q000379:methods*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D011557:Pseudorabies / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1
keywords: Antibody detection; ICS; PRV