title: Microbial community analysis in a combined anaerobic and aerobic digestion system for treatment of cellulosic ethanol production wastewater.
abstract: This study investigated the microbial diversity established in a combined system composed of a continuous stirred tank reactor (CSTR), expanded granular sludge bed (EGSB) reactor, and sequencing batch reactor (SBR) for treatment of cellulosic ethanol production wastewater. Excellent wastewater treatment performance was obtained in the combined system, which showed a high chemical oxygen demand removal efficiency of 95.8% and completely eliminated most complex organics revealed by gas chromatography-mass spectrometry (GC-MS). Denaturing gradient gel electrophoresis (DGGE) analysis revealed differences in the microbial community structures of the three reactors. Further identification of the microbial populations suggested that the presence of Lactobacillus and Prevotella in CSTR played an active role in the production of volatile fatty acids (VFAs). The most diverse microorganisms with analogous distribution patterns of different layers were observed in the EGSB reactor, and bacteria affiliated with Firmicutes, Synergistetes, and Thermotogae were associated with production of acetate and carbon dioxide/hydrogen, while all acetoclastic methanogens identified belonged to Methanosaetaceae. Overall, microorganisms associated with the ability to degrade cellulose, hemicellulose, and other biomass-derived organic carbons were observed in the combined system. The results presented herein will facilitate the development of an improved cellulosic ethanol production wastewater treatment system.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Shan
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.|School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Zebing
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Haiman
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: John J
authors.initials: JJ
authors.lastname: Ambuchi
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China. yujief@hit.edu.cn.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D002482:Cellulose / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D064307:Microbiota / Q000235:genetics*; D058889:Molecular Typing; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000032:analysis; D018508:Water Purification
chemical_list: D002482:Cellulose; D000431:Ethanol; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
keywords: Cellulosic ethanol; Continuous stirred tank reactor; Expanded granular sludge bed; Microbial community; Sequencing batch reactor; Wastewater treatment