title: Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities.
abstract: A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.
authors.affiliation: Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, United States.
authors.forename: Brett E
authors.initials: BE
authors.lastname: Arenz
authors.affiliation: Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, United States.
authors.forename: Dan C
authors.initials: DC
authors.lastname: Schlatter
authors.affiliation: Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, United States.
authors.forename: James M
authors.initials: JM
authors.lastname: Bradeen
authors.affiliation: Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, United States.
authors.forename: Linda L
authors.initials: LL
authors.lastname: Kinkel
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D002736:Chloroplasts / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D060026:Endophytes / Q000235:genetics* / Q000302:isolation & purification; D056186:Metagenomics / Q000379:methods*; D008928:Mitochondria / Q000235:genetics; D010944:Plants / Q000235:genetics / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant
keywords: Blocking; Chloroplast; Endophyte; Metagenomics; Mitochondria