title: Simultaneous determination of telmisartan and amlodipine in dog plasma by LC-MS-MS.
abstract: A simple, rapid and sensitive method was established for the simultaneous determination of telmisartan and amlodipine in dog plasma by a HPLC-MS-MS analysis. The plasma sample preparation was a simple deproteinization by the addition of three volumes of methanol/acetonitrile mixture followed by centrifugation. The analytes and internal standard diphenhydramine were separated on a Zorbax SB-C18 column with a mobile phase of acetonitrile : water (45 : 55, v/v) at a flow rate of 0.2 mL/min with an operating temperature of 25°C. Detection was carried out by electrospray ionization in positive-ion multiple reaction monitoring mode. The calibration plots in dog plasma were linear over the ranges of 0.5-2,000 ng/mL for telmisartan and 0.5-500 ng/mL for amlodipine. The lower limit of quantification was 0.5 ng/mL for two analytes. The intra- and interday precisions (RSD%) were within 9.0%. The average recoveries of analytes were >85.0%. The method was successfully applied to the pharmacokinetic study of the two compounds after oral administration of telmisartan-amlodipine combination preparation to dogs.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Perking Union Medical College, Beijing 100050, PR China.
authors.forename: Baolian
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Perking Union Medical College, Beijing 100050, PR China shengli@imm.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Sheng
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Perking Union Medical College, Beijing 100050, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D017311:Amlodipine / Q000097:blood*; D000818:Animals; D001562:Benzimidazoles / Q000097:blood*; D001565:Benzoates / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077333:Telmisartan
chemical_list: D001562:Benzimidazoles; D001565:Benzoates; D017311:Amlodipine; D000077333:Telmisartan