title: Effect of the molecular structure of lignin-based polyoxyethylene ether on enzymatic hydrolysis efficiency and kinetics of lignocelluloses.
abstract: Effect of the molecular structure of lignin-based polyoxyethylene ether (EHL-PEG) on enzymatic hydrolysis of Avicel and corn stover was investigated. With the increase of PEG contents and molecular weight of EHL-PEG, glucose yield of corn stover increased. EHL-PEG enhanced enzymatic hydrolysis of corn stover significantly at buffer pH 4.8-5.5. Glucose yield of corn stover at 20% solid content increased from 32.8% to 63.8% by adding EHL-PEG, while that with PEG4600 was 54.2%. Effect of EHL-PEG on enzymatic hydrolysis kinetics of cellulose film was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). An enhancing mechanism of EHL-PEG on enzymatic hydrolysis kinetics of cellulose was proposed. Cellulase aggregates dispersed by EHL-PEG excavated extensive cavities into the surface of cellulose film, making the film become more loose and exposed. After the maximum enzymatic hydrolysis rate, the film was mainly peeled off layer by layer until equilibrium.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Duming
authors.initials: D
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Ningxin
authors.initials: N
authors.lastname: Zhan
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Jieyi
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: cehmlou@scut.edu.cn.
authors.forename: Hongming
authors.initials: H
authors.lastname: Lou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Mingsong
authors.initials: M
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Yang
mesh_terms: D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D004987:Ethers / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D008031:Lignin / Q000737:chemistry*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry
chemical_list: D004987:Ethers; C036909:lignocellulose; D011092:Polyethylene Glycols; D002482:Cellulose; D008031:Lignin; D002480:Cellulase
keywords: Avicel; Corn stover; Enzymatic hydrolysis; Kinetic model; Lignin-based polyoxyethylene ether