title: Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report.
abstract: Leptospirosis is a zoonotic infection for which diagnosis is difficult. It has appeared as a global emerging infectious disease over recent years. Genotype determination often requires a Leptospira strain obtained by culture, which is a long and fastidious technique. A method based on multilocus variable number tandem repeat analysis (MLVA) to determine the genotype of Leptospira interrogans, performed directly on blood or urine samples, is proposed. This method was applied to a fatal case of leptospirosis for which the geographical origin of infection was unknown. This technique will allow a genotype to be obtained for L. interrogans, even when cultures remain negative.
authors.affiliation: CHU d'Angers, Laboratoire de Bactériologie, Institut de Biologie en Santé, Angers, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Pailhoriès
authors.affiliation: Service de Bactériologie-Virologie, Hôpital Bretonneau - CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Buzelé
authors.affiliation: Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Centre and WHO Collaborating Centre for Leptospirosis, Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Picardeau
authors.affiliation: Service de Bactériologie-Virologie, Hôpital Bretonneau - CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Robert
authors.affiliation: CHRU de Tours, Service de Réanimation Médicale, Tours, France; Clinical Research in Intensive Care and Sepsis (CRICS) Group, Tours, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Mercier
authors.affiliation: Service de Bactériologie-Virologie, Hôpital Bretonneau - CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, France; Université François Rabelais de Tours, UMR1282 ISP, Faculté de Médecine, Tours, France; INRA, UMR1282 ISP, Equipe Bactéries et Risque Materno-foetal, Nouzilly, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Mereghetti
authors.affiliation: Service de Bactériologie-Virologie, Hôpital Bretonneau - CHRU de Tours, 2 boulevard Tonnellé, F-37044 Tours, France; Université François Rabelais de Tours, UMR1282 ISP, Faculté de Médecine, Tours, France; INRA, UMR1282 ISP, Equipe Bactéries et Risque Materno-foetal, Nouzilly, France. Electronic address: philippe.lanotte@univ-tours.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lanotte
mesh_terms: D004587:Electrophoresis, Agar Gel; D017809:Fatal Outcome; D005838:Genotype; D006801:Humans; D007921:Leptospira interrogans / Q000235:genetics* / Q000302:isolation & purification; D007922:Leptospirosis / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing
keywords: Genotype; Leptospirosis; MLVA; VNTR