title: Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus.
abstract: Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain, and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Tan
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
authors.affiliation: Realbio Genomics Institute, Shanghai 200050, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Sheng
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China.
authors.forename: Beiwen
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Kunming Institute of Zoology, Chinese Academy of Science, Kunming 650223, China.
authors.forename: Zhanshan
authors.initials: Z
authors.lastname: Ma
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Peng
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Aiqun
authors.initials: A
authors.lastname: Jia
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D020869:Gene Expression Profiling; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D012329:RNA, Bacterial / Q000737:chemistry; D000077185:Resveratrol; D017423:Sequence Analysis, RNA; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013267:Stilbenes / Q000494:pharmacology; D059467:Transcriptome; D014315:Triterpenes / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology; D000097245:Ursolic Acid
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D000077185:Resveratrol; D013267:Stilbenes; D014315:Triterpenes; D014640:Vancomycin; D000097245:Ursolic Acid