title: Serum Lysozyme (Muramidase) Levels in Intra-Abdominal Abscesses: An Experimental Study.
abstract: The role of serum lysozyme in the presence of intra-abdominal sepsis was studied to act as an adjunct to various other modalities used for diagnosing intra-abdominal abscesses. Lysozyme (muramidase) is a bacteriolytic enzyme located within phagocytic cells including leukocytes. We measured serum concentrations of lysozyme by a standard turbidimetric method on three groups of murine models. One group underwent caecal ligation and puncture (CLP), the second group underwent a sham operation, and the third group was used as controls. In mice with intra-abdominal abscesses secondary to caecal ligation and perforation (n = 30), the serum lysozyme levels were increased compared to the sham-operated mice (n = 30) and controls (n = 30) (p = <0.001). In this study, serum lysozyme levels have a high sensitivity and specificity related to the presence of an intra-abdominal abscess in mice. Clinical studies are required to demonstrate its role in humans.
authors.affiliation: Department of Surgery, Christian Medical College and Hospital, Brown Road, Ludhiana, 141008 Punjab India.
authors.forename: Mathew P
authors.initials: MP
authors.lastname: Daniel
authors.affiliation: Department of Surgery, Christian Medical College and Hospital, Brown Road, Ludhiana, 141008 Punjab India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Gaikwad
authors.affiliation: Department of Surgery, Christian Medical College and Hospital, Brown Road, Ludhiana, 141008 Punjab India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Verghese
authors.affiliation: Department of Biochemistry, Christian Medical College and Hospital, Brown Road, Ludhiana, 141008 Punjab India.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Abraham
authors.affiliation: Department of Surgery, Christian Medical College and Hospital, Brown Road, Ludhiana, 141008 Punjab India.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Kapoor
keywords: Intra-abdominal abscesses; Lysozyme; Muramidase