title: Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment.
abstract: This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe(2+), Mg(2+), Ni(2+), and Zn(2+) further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe(2+) addition.
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R. China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R. China.
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R. China.|School of Environment and Resource, Renmin University of China, Beijing 100872, P.R. China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D000622:Aminolevulinic Acid / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D006863:Hydrogen-Ion Concentration; D008027:Light; D008670:Metals / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000254:growth & development* / Q000378:metabolism*; D014131:Trace Elements / Q000378:metabolism; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification
chemical_list: D000622:Aminolevulinic Acid; D008670:Metals; D014131:Trace Elements; D062065:Wastewater
keywords: 5-aminolevulinic acid; Rhodobacter sphaeroides;; influencing factors; optimization; wastewater treatment