title: Prevalence of ESBL and MBL encoding genes in Acinetobacter baumannii strains isolated from patients of intensive care units (ICU).
abstract: The aim of this study was to investigate the prevalence of ESBL and MBL encoding genes among A. baumannii isolates. In this cross sectional study, 100 A. baumannii strains were isolated from ICU wards of 3 educational hospitals of Hamadan City, Iran in 2011. Phenotypic identification of the production of ESBLs and MBLs has been carried out by using E-test and DDST methods, respectively. PCR technique was used for amplification of the ESBL and MBL encoding genes, namely: CTX-M, SHV, TEM, OXA-51, VIM-Family, IMP-Family, SPM-1, SIM-1, and GIM-1. Eighty seven (87%), 95 (95%), 98 (98%) and 95 (95%) out of 100 A. baumannii isolates were resistant to imipenem, meropenem, ceftazidime and cefotaxime, respectively. Also, 99% and 7% of the isolates were MBLs and ESBLs produced phenotypically. Thirty (30%), 20 (20%) and 58 (58%) out of 100 A. baumannii isolates have been confirmed to harbor the bla VIM-family, TEM and SHV genes, respectively. Our results show no significant relationship between the detected gens with production of MBLs and ESBLs in spite of high prevalence of MBL encoding and drug resistant A. baumannii. Probably some other genes rather than what we studied are involved in phenotypic production of MBLs and ESBLs and subsequent drug resistance in Hamadan area, Iran.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Safari
authors.affiliation: Young Researchers and Elite Club, Sari Branch, Islamic Azad University, Sari, Iran ; Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Amir Sasan
authors.initials: AS
authors.lastname: Mozaffari Nejad
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abas
authors.initials: A
authors.lastname: Bahador
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Rasool
authors.initials: R
authors.lastname: Jafari
authors.affiliation: Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ; Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad Yousef
authors.initials: MY
authors.lastname: Alikhani
keywords: Acinetobacter baumannii; Drug resistance; ESBL; MBL