title: Detection of methicillin-resistant Staphylococcus pseudintermedius ST169 and novel ST354 SCCmec II-III isolates related to the worldwide ST71 clone.
abstract: The recent appearance of methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a concern for both veterinary and human healthcare. MRSP clonal lineages with sequence type (ST) 71-spa t02-staphylococcal cassette chromosome mec (SCCmec) II-III and ST68-spa t06-SCCmec V have spread throughout Europe and North America, respectively. The current study compared the molecular characteristics of 43 MRSP isolates from dogs in Japan with those of MRSP from previous reports using multilocus sequence typing based on seven housekeeping genes, SCCmec typing, and detection of antimicrobial resistance genes. Three related clonal lineages, ST71, ST169, and the newly registered ST354, were observed in SCCmec II-III isolates from Japan, despite MRSP SCCmec II-III isolates being thought to belong to a single clonal lineage. The majority of SCCmec II-III isolates belonging to ST169 (9/11) and ST354 (3/3), but not ST71 (0/11), harboured tetM. Four STs were observed for the SCCmec V isolates; however, neither ST68 nor related STs were found in the Japanese MRSP isolates. In conclusion, MRSP SCCmec II-III isolates from Japan belonged to ST71 and related STs (ST169 and ST354). A variety of MRSP SCCmec V clones, including some novel clones, were identified.
authors.affiliation: Department of Veterinary Medicine,Tokyo University of Agriculture and Technology,Fuchu,Tokyo,Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.affiliation: School of Veterinary Medicine,Rakuno Gakuen University,Ebetsu,Hokkaido,Japan.
authors.forename: A
authors.initials: A
authors.lastname: Koizumi
authors.affiliation: School of Veterinary Medicine,Rakuno Gakuen University,Ebetsu,Hokkaido,Japan.
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.affiliation: School of Veterinary Medicine,Rakuno Gakuen University,Ebetsu,Hokkaido,Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Muramatsu
authors.affiliation: School of Veterinary Medicine,Rakuno Gakuen University,Ebetsu,Hokkaido,Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology; D004285:Dogs; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D007564:Japan; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000662:veterinary; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008712:Methicillin
keywords: Japan; MLST; Staphylococcus pseudintermedius; methicillin resistance