title: Preliminary investigation of differential tapelifting for sampling forensically relevant layered deposits.
abstract: The analysis of DNA mixtures can be problematic, especially when in trace quantities such as when a biological sample is deposited onto a substrate which contains background DNA (for example, in the case of touch DNA deposited onto a garment containing the wearer's DNA). We conducted a preliminary investigation into the possibility of removing such multi-donor deposits layer by layer using a differential tape-lifting method. Two types of tape were tested using two different numbers of applications for sampling layered deposits of touch DNA/touch DNA and touch DNA/saliva, both on the same polyester-cotton plain woven material. The data showed that there was no significant increase in the ratio of secondary to primary deposit when sampled in this manner, compared to direct extraction from cuttings of the touched fabric. A similar result was also obtained even when the deposits were on opposing surfaces of the fabric and the sampling was carried out on the secondary deposit side. These findings indicate that biological material, whether touch DNA or saliva, does not predominantly remain on the side of the fabric on which it is deposited (at least for plain-woven polyester-cotton). They also highlight the importance of considering substrate properties when making assumptions as to the resulting location of biological materials from a deposition event, and the necessity to conduct further research on the interactions between substrates and deposits.
authors.affiliation: Office of the Chief Forensic Scientist, Victoria Police Forensic Services Centre, 31 Forensic Drive, Macleod, Victoria 3085, Australia; Department of Genetics, La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, Victoria 3086, Australia. Electronic address: timjverdon@gmail.com.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Verdon
authors.affiliation: Department of Genetics, La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, Victoria 3086, Australia.
authors.forename: R John
authors.initials: RJ
authors.lastname: Mitchell
authors.affiliation: Office of the Chief Forensic Scientist, Victoria Police Forensic Services Centre, 31 Forensic Drive, Macleod, Victoria 3085, Australia.
authors.forename: Roland A H
authors.initials: RA
authors.lastname: van Oorschot
mesh_terms: D000269:Adhesives; D004247:DNA / Q000302:isolation & purification*; D005554:Forensic Medicine; D006801:Humans; D013048:Specimen Handling / Q000379:methods*; D014110:Touch
chemical_list: D000269:Adhesives; D004247:DNA
keywords: DNA mixtures; DNA sampling; Differential sampling; Forensic biology; Tapelifting; Targeted sampling; Trace DNA