title: Unravelling the one-carbon metabolism of the acetogen Sporomusa strain An4 by genome and proteome analysis.
abstract: The Sporomusa genus comprises anaerobic spore-forming acetogenic bacteria that stain Gram-negative. Sporomusa species typically grow with one-carbon substrates and N-methylated compounds. In the degradation of these compounds methyltransferases are involved. In addition, Sporomusa species can grow autotrophically with H2 and CO2 , and use a variety of sugars for acetogenic growth. Here we describe a genome analysis of Sporomusa strain An4 and a proteome analysis of cells grown under five different conditions. Comparison of the genomes of Sporomusa strain An4 and Sporomusa ovata strain H1 indicated that An4 is a S. ovata strain. Proteome analysis showed a high abundance of several methyltransferases, predominantly trimethylamine methyltransferases, during growth with betaine, whereas trimethylamine is one of the main end-products of betaine degradation. In methanol degradation methyltransferases are also involved. In methanol-utilizing methanogens, two methyltransferases catalyse methanol conversion, methyltransferase 1 composed of subunits MtaB and MtaC and methyltransferase 2, also called MtaA. The two methyltransferase 1 subunits MtaB and MtaC were highly abundant when strain An4 was grown with methanol. However, instead of MtaA a methyltetrahydrofolate methyltransferase was synthesized. We propose a novel methanol degradation pathway in Sporomusa strain An4 that uses a methyltetrahydrofolate methyltransferase instead of MtaA.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands. michael.visser@wur.nl.
authors.forename: Michael
authors.initials: M
authors.lastname: Visser
authors.affiliation: Department of Biotechnology, Technische Universiteit Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands.|Netherlands Proteomics Centre, Julianalaan 67, 2628 BC, Delft, The Netherlands.
authors.forename: Mervin M
authors.initials: MM
authors.lastname: Pieterse
authors.affiliation: Department of Biotechnology, Technische Universiteit Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands.|Netherlands Proteomics Centre, Julianalaan 67, 2628 BC, Delft, The Netherlands.
authors.forename: Martijn W H
authors.initials: MW
authors.lastname: Pinkse
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: Nijsse
authors.affiliation: Department of Biotechnology, Technische Universiteit Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands.|Netherlands Proteomics Centre, Julianalaan 67, 2628 BC, Delft, The Netherlands.
authors.forename: Peter D E M
authors.initials: PD
authors.lastname: Verhaert
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.|Department of Veterinary Biosciences, Helsinki University, Helsinki, Finland.|Department of Bacteriology and Immunology, Helsinki University, Helsinki, Finland.
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schaap
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.|CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Stams
mesh_terms: D001622:Betaine / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D016680:Genome, Bacterial; D000432:Methanol / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D020543:Proteome; D045854:Veillonellaceae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D008744:Methylamines; D020543:Proteome; D001622:Betaine; D002244:Carbon; D008780:Methyltransferases; C023336:trimethylamine; D000432:Methanol