title: A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.
abstract: Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. nataliacybelle.89@gmail.com.
authors.forename: Natália
authors.initials: N
authors.lastname: Oliveira
authors.affiliation: Universidade Federal de Alagoas (UFAL), Campus Arapiraca, Av. Manoel Severino Barbosa, s/n, Bom Sucesso, 57.309-005 Arapiraca, AL, Brazil. elainevms@yahoo.com.br.
authors.forename: Elaine
authors.initials: E
authors.lastname: Souza
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. daniellylsantos@hotmail.com.
authors.forename: Danielly
authors.initials: D
authors.lastname: Ferreira
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. deborahzanforin@gmail.com.
authors.forename: Deborah
authors.initials: D
authors.lastname: Zanforlin
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. wessullas@yahoo.com.br.
authors.forename: Wessulla
authors.initials: W
authors.lastname: Bezerra
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. mariameliaborba@gmail.com.
authors.forename: Maria Amélia
authors.initials: MA
authors.lastname: Borba
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. mariana.s.arruda@gmail.com.
authors.forename: Mariana
authors.initials: M
authors.lastname: Arruda
authors.affiliation: Departamento de Virologia e Terapia Experimental (LAVITE), Centro de Pesquisas Aggeu Magalhães (CPqAM), Fundação Oswaldo Cruz (Fiocruz)-Pernambuco, Av. Professor Moraes Rego, s/n, Campus da UFPE, 50.670-420 Recife, PE, Brazil. kennya.genne@bol.com.br.
authors.forename: Kennya
authors.initials: K
authors.lastname: Lopes
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. galvesn23@gmail.com.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Nascimento
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. Marli@cpqam.fiocruz.br.|Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Professor Moraes Rego, s/n, Campus da UFPE, CEP: 50670-901 Recife, PE, Brazil. Marli@cpqam.fiocruz.br.
authors.forename: Danyelly
authors.initials: D
authors.lastname: Martins
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Professor Moraes Rego, s/n, Campus da UFPE, CEP: 50670-901 Recife, PE, Brazil. Marli@cpqam.fiocruz.br.
authors.forename: Marli
authors.initials: M
authors.lastname: Cordeiro
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. Marli@cpqam.fiocruz.br.|Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Professor Moraes Rego, s/n, Campus da UFPE, CEP: 50670-901 Recife, PE, Brazil. Marli@cpqam.fiocruz.br.
authors.forename: José
authors.initials: J
authors.lastname: Lima-Filho
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000097:blood / Q000235:genetics*; D003715:Dengue; D003716:Dengue Virus / Q000235:genetics*; D055664:Electrochemical Techniques; D006147:Guanine; D006801:Humans; D057230:Limit of Detection; D010084:Oxidation-Reduction
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D006147:Guanine
keywords: DNA biosensors; dengue fever; differential pulse voltammetry; electrochemical detection; guanine oxidation