title: Cross-talks between microRNAs and mRNAs in pancreatic tissues of streptozotocin-induced type 1 diabetic mice.
abstract: Network cross-talks between microRNAs (miRNAs) and mRNAs may be useful to elucidate the pathological mechanisms of pancreatic islet cells in diabetic individuals. The aim of the present study was to investigate the cross-talks between miRNAs and mRNAs in pancreatic tissues of streptozotocin-induced diabetic mice through microarray and bioinformatic methods. Based on the miRNA microarray, 64 upregulated and 72 downregulated miRNAs were observed in pancreatic tissues in diabetic mice compared to the normal controls. Based on the mRNA microarrray, 507 upregulated mRNAs and 570 downregulated mRNAs were identified in pancreatic tissues in diabetic mice compared to the normal controls. Notably, there were 246 binding points between upregulated miRNA and downregulated mRNAs; simultaneously, there were 583 binding points between downregulated miRNA and upregulated mRNAs. These changed mRNA may potentially involve the following signaling pathways: Insulin secretion, pancreatic secretion, mammalian target of rapamycin signaling pathway, forkhead box O signaling pathway and phosphatidylinositol 3-kinase-protein kinase B signaling. The fluctuating effects of miRNAs and matched mRNAs indicated that miRNAs may have wide cross-talks with mRNAs in pancreatic tissues of type 1 diabetic mice. The cross-talks may play important roles in contributing to impaired islet functions and the development of diabetes. However, further functional validation should be conducted in the future.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, P.R. China ; Shenzhen Key Laboratory of Health Science and Technology, Division of Life Science and Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, P.R. China.
authors.forename: Caiming
authors.initials: C
authors.lastname: Tian
authors.affiliation: Department of Health Inspection and Quarantine, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, P.R. China ; School of Life Sciences, Tsinghua University, Beijing 100084, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Shenzhen Key Laboratory of Health Science and Technology, Division of Life Science and Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, P.R. China.
authors.forename: Yaou
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shenzhen Key Laboratory of Health Science and Technology, Division of Life Science and Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, P.R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Xie
keywords: diabetes; islet cells; microRNA; microarray; network