title: The Impact of Gene Polymorphisms on the Success of Anticholinergic Treatment in Children with Overactive Bladder.
abstract: OBJECTIVE
  To determine the impact of gene polymorphisms on detrusor contraction-relaxation harmony in children with lower urinary tract symptoms (LUTS).
  
  
  METHODS
  Toilet trained children older than 5 years of age with LUTS and normal neurological examination underwent videourodynamic study. The control group was composed of age matched children with no voiding complaints. The study group who filled out the voiding dysfunction symptom score before and after the treatment received standard oxybutynin treatment and was reevaluated 1 year after treatment. Genomic DNA was isolated from all patients and subjected to PCR for amplification. Genotyping of ARGHEF10, ROCK2, ADRB3, and CYP3A4 was carried out with Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  34 (45%) and 42 (55%) patients were enrolled in the study and control group, respectively. ARGEF10 GG, ADRB3 TC, and CYP3A4 AG genotype patients displayed insignificant difference between pre- and posttreatment voiding dysfunction symptom score and bladder volumes.
  
  
  CONCLUSIONS
  The polymorphism of genes in the cholinergic pathway did not significantly differ clinical parameters. On the other hand, polymorphic patients in the adrenergic pathway seemed to suffer from clinical disappointment. For this reason, we think that the neglected adrenergic pathway could be a new therapeutic target for the treatment of anticholinergic resistant LUTS in children.
authors.affiliation: Department of Urology, Gazi University School of Medicine, 06500 Ankara, Turkey ; Department of Medical Biology and Genetics, Gazi University School of Medicine, 06500 Ankara, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Gurocak
authors.affiliation: Department of Medical Biology and Genetics, Gazi University School of Medicine, 06500 Ankara, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Konac
authors.affiliation: Department of Urology, Osmangazi University School of Medicine, 26040 Eskisehir, Turkey.
authors.forename: Iyimser
authors.initials: I
authors.lastname: Ure
authors.affiliation: Department of Urology, Gazi University School of Medicine, 06500 Ankara, Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Senol
authors.affiliation: Department of Medical Biology and Genetics, Gazi University School of Medicine, 06500 Ankara, Turkey.
authors.forename: Ilke Hacer
authors.initials: IH
authors.lastname: Onen
authors.affiliation: Department of Urology, Gazi University School of Medicine, 06500 Ankara, Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Sozen
authors.affiliation: Department of Medical Biology and Genetics, Gazi University School of Medicine, 06500 Ankara, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Menevse
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D018680:Cholinergic Antagonists / Q000627:therapeutic use*; D051544:Cytochrome P-450 CYP3A / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008333:Mandelic Acids / Q000627:therapeutic use*; D012150:Polymorphism, Restriction Fragment Length; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics; D064067:Rho Guanine Nucleotide Exchange Factors / Q000235:genetics; D016896:Treatment Outcome; D053201:Urinary Bladder, Overactive / Q000188:drug therapy* / Q000235:genetics; D054460:rho-Associated Kinases / Q000235:genetics
chemical_list: C568534:ADRB3 protein, human; C577232:ARHGEF10 protein, human; D018680:Cholinergic Antagonists; D008333:Mandelic Acids; D022702:Receptors, Adrenergic, beta-3; D064067:Rho Guanine Nucleotide Exchange Factors; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases; C005419:oxybutynin