title: Bacteriological assessment of the hospital environment in two referral hospitals in Yaoundé-Cameroon.
abstract: BACKGROUND
  Many studies still show significant numbers of surgical patients contracting nosocomial infections each year globally with high morbidity and mortality. The aim of this study was to identify potential bacteria reservoirs that may be responsible for nosocomial infection in surgical services in the Yaoundé University Teaching Hospital (YUTH) and the Central Hospital Yaoundé (CHY).
  
  
  METHODS
  A cross sectional descriptive study was conducted from June to August 2012. Air, water, and surface samples were collected from two surgical services and subjected to standard bacteriological analysis.
  
  
  RESULTS
  A total of 143 surface samples were collected. Bacteria were isolated in all surfaces except from one trolley sample and a surgical cabinet sample. The predominant species in all services was coagulase negative Staphylococcus (CNS). The average number of colonies was 132. 82 CFU/25 cm(2). The bacteria isolated in the air were similar to those isolated from surfaces. From the 16 water samples cultured, an average of 50.93 CFU/100ml bacteria were isolated. The distribution of isolated species showed a predominance of Burkholderia cepacia.
  
  
  CONCLUSIONS
  These results showed the importance of the hospital environment as a potential reservoir and source of nosocomial infections amongst surgical patient at YUTH and CHY, thus we suggest that Public health policy makers in Cameroon must define, publish guidelines and recommendations for monitoring environmental microbiota in health facilities.
authors.affiliation: Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Kamga Hortense
authors.initials: KH
authors.lastname: Gonsu
authors.affiliation: School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon.
authors.forename: Etienne
authors.initials: E
authors.lastname: Guenou
authors.affiliation: Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Michel
authors.initials: M
authors.lastname: Toukam
authors.affiliation: Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Valantine Ngum
authors.initials: VN
authors.lastname: Ndze
authors.affiliation: Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Calixte Didier
authors.initials: CD
authors.lastname: Mbakop
authors.affiliation: Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Dongmo Norbert
authors.initials: DN
authors.lastname: Tankeu
authors.affiliation: Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Francois Xavier
authors.initials: FX
authors.lastname: Mbopi-Keou
authors.affiliation: Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Samuel
authors.initials: S
authors.lastname: Takongmo
mesh_terms: D000391:Air Microbiology; D001419:Bacteria / Q000302:isolation & purification; D002163:Cameroon; D003428:Cross Infection / Q000382:microbiology*; D003430:Cross-Sectional Studies; D004866:Equipment Contamination; D004865:Equipment and Supplies, Hospital / Q000382:microbiology*; D006761:Hospitals; D006801:Humans; D014871:Water Microbiology
keywords: Cameroon; Hospital environnement; nosocomial infection; reservoir; surgery