title: Enzyme Inhibitory Radicinol Derivative from Endophytic fungus Bipolaris sorokiniana LK12, Associated with Rhazya stricta.
abstract: Endophytes, living inside plant tissues, play an essential role in plant growth and development, whilst producing unique bioactive secondary metabolites. In the current study, the endophytic fungus Bipolaris sorokiniana LK12 was isolated from the leaves of ethno-medicinal and alkaloidal rich Rhazya stricta. The bulk amount of ethyl acetate extract of fungus was subjected to advance column chromatographic techniques, which resulted in the isolation of a new radicinol derivative, bipolarisenol (1). It was found to be a derivative of radicinol. The structure elucidation was carried out by the combined use of 1D and 2D nuclear magnetic resonance, infrared spectroscopy, mass, and UV spectrometric analyses. The bipolarisenol was assessed for its potential role in enzyme inhibition of urease and acetyl cholinesterase (AChE). Results showed that bipolarisenol significantly inhibited the AChE activity with low IC50 (67.23 ± 5.12 µg·mL-1). Bipolarisenol inhibited urease in a dose-dependent manner with high IC50 (81.62 ± 4.61 µg·mL-1). The new compound also showed a moderate anti-lipid peroxidation potential (IC50 = 168.91 ± 4.23 µg·mL-1). In conclusion, endophytes isolated from medicinal plants possess a unique potential to be considered for future drug discovery.
authors.affiliation: UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. abdullatif@unizwa.edu.om.
authors.forename: Abdul Latif
authors.initials: AL
authors.lastname: Khan
authors.affiliation: UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. malikhejric@gmail.com.
authors.forename: Liaqat
authors.initials: L
authors.lastname: Ali
authors.affiliation: UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. javidhej@unizwa.edu.om.|Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. javidhej@unizwa.edu.om.
authors.forename: Javid
authors.initials: J
authors.lastname: Hussain
authors.affiliation: UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. tania@unizwa.edu.om.
authors.forename: Tania Shamim
authors.initials: TS
authors.lastname: Rizvi
authors.affiliation: UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. aharrasi@unizwa.edu.om.|Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman. aharrasi@unizwa.edu.om.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Harrasi
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea. ijlee@knu.ac.kr.
authors.forename: In-Jung
authors.initials: IJ
authors.lastname: Lee
mesh_terms: D029062:Apocynaceae / Q000382:microbiology*; D001203:Ascomycota / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D011753:Pyrones / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D011753:Pyrones; C487590:radicinol
keywords: Bipolaris sorokiniana LK12; Rhazya stricta; bipolarisenol; enzyme inhibition; lipid peroxidation; radicinol