title: Protection of retinal function by sulforaphane following retinal ischemic injury.
abstract: Sulforaphane, a precursor of glucosinolate in cruciferous vegetables such as broccoli and cauliflower, has been shown to protect brain ischemic injury. In this study, we examined the effect of systemic administration of sulforaphane on retinal ischemic reperfusion injury. Intraocular pressure was elevated in two groups of C57BL/6 mice (n = 8 per group) for 45 min to induce retinal ischemic reperfusion injury. Following retinal ischemic reperfusion injury, vehicle (1% DMSO saline) or sulforaphane (25 mg/kg/day) was administered intraperitoneally daily for 5 days. Scotopic electroretinography (ERG) was used to quantify retinal function prior to and one-week after retinal ischemic insult. Retinal morphology was examined one week after ischemic insult. Following ischemic reperfusion injury, ERG a- and b-wave amplitudes were significantly reduced in the control mice. Sulforaphane treatment significantly attenuated ischemic-induced loss of retinal function as compared to vehicle treated mice. In vehicle treated mice, ischemic reperfusion injury produced marked thinning of the inner retinal layers, but the thinning of the inner retinal layers appeared significantly less with sulforaphane treatment. Thus, sulforaphane may be beneficial in the treatment of retinal disorders with ischemic reperfusion injury.
authors.affiliation: Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA.
authors.forename: Lindsay A
authors.initials: LA
authors.lastname: Ambrecht
authors.affiliation: Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA; Department of Pathology, Loyola University Chicago, Maywood, IL 60153, USA; Surgery Service, Edward Hines, Jr. VA Hospital, Hines, IL 60141, USA.
authors.forename: Jay I
authors.initials: JI
authors.lastname: Perlman
authors.affiliation: Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA.
authors.forename: James F
authors.initials: JF
authors.lastname: McDonnell
authors.affiliation: Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA.
authors.forename: Yougang
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA; Research Service, Edward Hines, Jr. VA Hospital, Hines, IL 60141, USA. Electronic address: pbu@luc.edu.
authors.forename: Ping
authors.initials: P
authors.lastname: Bu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004596:Electroretinography; D007274:Injections, Intraperitoneal; D017879:Isothiocyanates / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D012160:Retina / Q000503:physiopathology*; D012164:Retinal Diseases / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D013454:Sulfoxides
chemical_list: D017879:Isothiocyanates; D018696:Neuroprotective Agents; D013454:Sulfoxides; C016766:sulforaphane
keywords: Electroretinography; Neuroprotection; Retinal ischemic reperfusion injury; Retinal morphology; Sulforaphane