title: Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.
abstract: The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shiraiwa
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Eguchi
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hikono
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kusumoto
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan; Research Institute for Biological Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address: shimoji@affrc.go.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004888:Erysipelothrix / Q000145:classification* / Q000235:genetics*; D004889:Erysipelothrix Infections / Q000382:microbiology*; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial
keywords: Erysipelothrix rhusiopathiae; Genome sequencing; SNP-based PCR; Strain-specific detection; Vaccine strain