title: Real-Time Visualization of Platelet Interaction With Micro Structured Surfaces.
abstract: Improving the hemocompatibility of artificial implants by micro structuring their surfaces has shown promising results, but the mechanisms which lead to this improvement are not yet understood. Therefore, we built a test setup for real-time visualization of platelet interaction with a plain and two micro structured surfaces. The micro structures, defined by the distance of the plain surface area between the structures, were chosen to be 3 and 30 μm, representing a positive and a negative effect on the hemocompatibility. The main part of the test setup was a flow chamber containing films of low density polyethylene (LDPE) with the differently structured surfaces. For different wall shear stresses, no considerable differences were observed in the platelet-surface interaction for all surface types. Whereas, major differences in flow behavior were observed when comparing the surfaces to each other. The platelets "rolled" along the smooth surface, being in constant contact with the surface material. Although the platelets "rolled" over the surface with small structures as well, they were only in contact with the tips of the structure and therefore had less surface contact with the foreign material. The increased distance and height of the structures of the last surface led to a trapping of platelets between the structures. This resulted in a longer contact time with the foreign material as well as a larger contact area, which both increase the risk of platelet activation, adhesion, and finally clotting. Our results showed the mechanisms which lead to these effects and thus revealed why micro structuring of surfaces impacts the hemocompatibility. Furthermore, we established a test setup which can be used for future investigations on the platelet-structure interactions.
authors.affiliation: Department of Cardiovascular Engineering, Institute of Applied Medical Engineering-Helmholtz Institute-RWTH Aachen University, Aachen, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Gester
authors.affiliation: Department of Cardiovascular Engineering, Institute of Applied Medical Engineering-Helmholtz Institute-RWTH Aachen University, Aachen, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Birtel
authors.affiliation: Department of Cardiovascular Engineering, Institute of Applied Medical Engineering-Helmholtz Institute-RWTH Aachen University, Aachen, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Clauser
authors.affiliation: Department of Cardiovascular Engineering, Institute of Applied Medical Engineering-Helmholtz Institute-RWTH Aachen University, Aachen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Steinseifer
authors.affiliation: Department of Cardiovascular Engineering, Institute of Applied Medical Engineering-Helmholtz Institute-RWTH Aachen University, Aachen, Germany.
authors.forename: Simon Johannes
authors.initials: SJ
authors.lastname: Sonntag
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001792:Blood Platelets / Q000166:cytology*; D008422:Materials Testing; D010973:Platelet Adhesiveness; D020959:Polyethylene / Q000737:chemistry; D019736:Prostheses and Implants; D013499:Surface Properties; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D020959:Polyethylene
keywords: Flow chamber; Hemocompatibility; Live cell imaging; Micro structured surfaces; Visualization of platelets