title: Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows.
abstract: The objective of this study was to evaluate the progression of the uterine microbiota from calving until establishment of metritis. Uterine swabs (n = 72) collected at 0, 2, and 6 ± 2 days postpartum (dpp) from 12 metritic and 12 healthy cows were used for metagenomic sequencing of the 16S rRNA gene on the Illumina MiSeq platform. A heat map showed that uterine microbiota was established at calving. The microbiota changed rapidly from 0 to 6 ± 2 dpp, with a decrease in the abundance of Proteobacteria and an increase in the abundance of Bacteroidetes and Fusobacteria, which were dominant in metritic cows. Uterine microbiota composition was shared; however, metritic and healthy cows could be discriminated using relative abundance of bacterial genera at 0, 2, and 6 ± 2 dpp. Bacteroides was the main genus associated with metritis because it was the only genus that showed significantly greater abundance in cows with metritis. As the abundance of Bacteroides organisms increased, the uterine discharge score, a measure of uterine health, worsened. Fusobacterium was also an important genus associated with metritis because Fusobacterium abundance increased as Bacteroides abundance increased and the uterine discharge score worsened as the abundance increased. The correlation with uterine discharge score and the correlation with Bacteroides or Fusobacterium showed that other bacteria, such as Helcoccocus, Filifactor, and Porphyromonas, were also associated with metritis. There were also bacteria associated with uterine health, such as "Candidatus Blochmannia," Escherichia, Sneathia, and Pedobacter.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Jeon
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
authors.forename: Achilles
authors.initials: A
authors.lastname: Vieira-Neto
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Mohanathas
authors.initials: M
authors.lastname: Gobikrushanth
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Daetz
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
authors.forename: Rodolfo D
authors.initials: RD
authors.lastname: Mingoti
authors.affiliation: Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Ana Carolina Brigolin
authors.initials: AC
authors.lastname: Parize
authors.affiliation: Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Sabrina Lucas
authors.initials: SL
authors.lastname: de Freitas
authors.affiliation: Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Antonio Nelson Lima
authors.initials: AN
authors.lastname: da Costa
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
authors.forename: Rodrigo C
authors.initials: RC
authors.lastname: Bicalho
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Lima
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida, USA Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA.
authors.forename: K Casey
authors.initials: KC
authors.lastname: Jeong
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, Florida, USA galvaok@ufl.edu.
authors.forename: Klibs N
authors.initials: KN
authors.lastname: Galvão
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D041963:Bacteroidetes / Q000302:isolation & purification; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000382:microbiology*; D004716:Endometritis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D045182:Fusobacteria / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing; D056265:Microbial Interactions; D064307:Microbiota; D049590:Postpartum Period; D020560:Proteobacteria / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013997:Time Factors; D014599:Uterus / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S