title: Efficacy of hyperbaric oxygen therapy combined with mild hyperthermia for improving the anti-tumour effects of carboplatin.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of hyperbaric oxygen therapy (HBO) on the enhancement of hyperthermic chemosensitisation to carboplatin at mild temperatures in experimental tumours.
  
  
  METHODS
  SCCVII carcinoma in C3H/He mice was used to assess tumour growth delay. The mice received intraperitoneal injections of carboplatin. For HBO treatment, the mice were exposed to HBO at 2.0 atmospheres of absolute oxygen for 60 min. For mild hyperthermia (HT), treatment at 41.5 °C for 30 min was performed. The tumour tissue pO2 levels were measured with a digital pO2 monitor during and immediately after treatment.
  
  
  RESULTS
  The average time taken to reach a threefold relative tumour size was significantly longer after treatment with carboplatin combined with mild HT and HBO than after treatment with carboplatin and mild HT. The relative sizes of the tumours after the combined treatment were smallest when the treatment sequence was carboplatin, mild HT, and HBO. The tumour tissue pO2 values were significantly higher immediately after mild HT followed by HBO than immediately after HBO followed by mild HT. The tumour tissue pO2 levels during mild HT and HBO generally increased, although the patterns of the increases varied.
  
  
  CONCLUSIONS
  The administration of HBO increased the effects of hyperthermic chemosensitisation to carboplatin at mild temperatures on experimental tumours, particularly when given in the sequence of carboplatin, mild HT, and HBO, a finding that supports previous clinical outcomes for a novel combined therapy using carboplatin plus HT and HBO.
authors.affiliation: a Department of Radiology , University of Occupational and Environmental Health , Kitakyushu , Japan .
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ohguri
authors.affiliation: b Department of Environmental Health , National Institute of Public Health , Wako , Japan .
authors.forename: Naoki
authors.initials: N
authors.lastname: Kunugita
authors.affiliation: a Department of Radiology , University of Occupational and Environmental Health , Kitakyushu , Japan .
authors.forename: Katsuya
authors.initials: K
authors.lastname: Yahara
authors.affiliation: c Department of Cancer Therapy Centre , Tobata Hospital , Kitakyushu , Japan .
authors.forename: Hajime
authors.initials: H
authors.lastname: Imada
authors.affiliation: d Department of Medical Electronics , University of Occupational and Environmental Health , Kitakyushu , Japan .
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Uemura
authors.affiliation: c Department of Cancer Therapy Centre , Tobata Hospital , Kitakyushu , Japan .
authors.forename: Nadayoshi
authors.initials: N
authors.lastname: Shinya
authors.affiliation: c Department of Cancer Therapy Centre , Tobata Hospital , Kitakyushu , Japan .
authors.forename: Gotou
authors.initials: G
authors.lastname: Youjirou
authors.affiliation: c Department of Cancer Therapy Centre , Tobata Hospital , Kitakyushu , Japan .
authors.forename: Chijiwa
authors.initials: C
authors.lastname: Takashi
authors.affiliation: e Department of Radiological Health Science , University of Occupational and Environmental Health , Kitakyushu , Japan , and.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Okazaki
authors.affiliation: f Department of Radiation Biology and Health , University of Occupational and Environmental Health , Kitakyushu , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ootsuyama
authors.affiliation: a Department of Radiology , University of Occupational and Environmental Health , Kitakyushu , Japan .
authors.forename: Yukunori
authors.initials: Y
authors.lastname: Korogi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D016190:Carboplatin / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism / Q000628:therapy*; D003131:Combined Modality Therapy; D005260:Female; D006931:Hyperbaric Oxygenation; D006979:Hyperthermia, Induced; D008809:Mice, Inbred C3H; D010100:Oxygen / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D016190:Carboplatin; D010100:Oxygen
keywords: Carboplatin; chemotherapy; hyperbaric oxygen; hyperthermia