title: A target-induced three-way G-quadruplex junction for 17β-estradiol monitoring with a naked-eye readout.
abstract: A label-free and enzyme-free three-way G-quadruplex junction sensing system for the amplified detection of 17β-estradiol has been constructed by the ingenious coupling of split G-quadruplex DNAzyme with toehold-mediated strand displacement. The biosensor is ultrasensitive, enabling the visual detection of 17β-estradiol concentrations as low as 1 fM without instrumentation.
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China. sgzhou@soil.gd.cn.
authors.forename: Junhua
authors.initials: J
authors.lastname: Chen
authors.forename: Junlin
authors.initials: J
authors.lastname: Wen
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yang
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
mesh_terms: D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry; D004958:Estradiol / Q000032:analysis*; D004967:Estrogens / Q000032:analysis*; D054856:G-Quadruplexes
chemical_list: D021881:DNA, Catalytic; D004967:Estrogens; D004958:Estradiol