title: Imaging membrane remodeling during regulated exocytosis in live mice.
abstract: In this mini-review we focus on the use of time-lapse light microscopy to study membrane remodeling during protein secretion in live animals. In particular, we highlight how subcellular intravital microscopy has enabled imaging the dynamics of both individual secretory vesicles and the plasma membrane, during different steps in the exocytic process. This powerful approach has provided us with the unique opportunity to unravel the role of the actin cytoskeleton in regulating this process under physiological conditions, and to overcome the shortcomings of more reductionist model systems.
authors.affiliation: Intracellular Membrane Trafficking Section, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, Bethesda, MD 20892-4340, United States.
authors.forename: Akiko
authors.initials: A
authors.lastname: Shitara
authors.affiliation: Intracellular Membrane Trafficking Section, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, Bethesda, MD 20892-4340, United States. Electronic address: weigertr@mail.nih.gov.
authors.forename: Roberto
authors.initials: R
authors.lastname: Weigert
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D005089:Exocytosis / Q000502:physiology*; D051379:Mice; D059008:Time-Lapse Imaging / Q000379:methods*