title: Preliminary In Vitro Assessment of Stem Cell Compatibility with Cross-Linked Poly(ε-caprolactone urethane) Scaffolds Designed through High Internal Phase Emulsions.
abstract: By using a high internal phase emulsion process, elastomeric poly(ε-caprolactone urethane) (PCLU) scaffolds were designed with pores size ranging from below 150 μm to 1800 μm and a porosity of 86% making them suitable for bone tissue engineering applications. Moreover, the pores appeared to be excellently interconnected, promoting cellularization and future bone ingrowth. This study evaluated the in vitro cytotoxicity of the PCLU scaffolds towards human mesenchymal stem cells (hMSCs) through the evaluation of cell viability and metabolic activity during extract test and indirect contact test at the beginning of the scaffold lifetime. Both tests demonstrated that PCLU scaffolds did not induce any cytotoxic response. Finally, direct interaction of hMSCs and PCLU scaffolds showed that PCLU scaffolds were suitable for supporting the hMSCs adhesion and that the cells were well spread over the pore walls. We conclude that PCLU scaffolds may be a good candidate for bone tissue regeneration applications using hMSCs.
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, France ; Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 74 rue Marcel Cachin, 93017 Bobigny, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Changotade
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, France ; Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 74 rue Marcel Cachin, 93017 Bobigny, France.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Radu Bostan
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, France ; Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 74 rue Marcel Cachin, 93017 Bobigny, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Consalus
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, France ; Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 74 rue Marcel Cachin, 93017 Bobigny, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Poirier
authors.affiliation: Institut de Recherche Biomédicale des Armées, Unité de Thérapie Cellulaire et Réparation Tissulaire, Site du Centre de Transfusion Sanguine des Armées "Jean Julliard" de Clamart, BP 73 91223 Brétigny-sur-Orge Cedex, France ; Ecole du Val de Grâce, 1 place Alphonse Lavéran, 75005 Paris Cedex, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Peltzer
authors.affiliation: Institut de Recherche Biomédicale des Armées, Unité de Thérapie Cellulaire et Réparation Tissulaire, Site du Centre de Transfusion Sanguine des Armées "Jean Julliard" de Clamart, BP 73 91223 Brétigny-sur-Orge Cedex, France ; Ecole du Val de Grâce, 1 place Alphonse Lavéran, 75005 Paris Cedex, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Lataillade
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, France ; Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 74 rue Marcel Cachin, 93017 Bobigny, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Lutomski
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 99 avenue JB Clément, 93430 Villetaneuse, France ; Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, UMR CNRS 7244, 74 rue Marcel Cachin, 93017 Bobigny, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Rohman