title: Influence of the antimicrobial compound allyl isothiocyanate against the Aspergillus parasiticus growth and its aflatoxins production in pizza crust.
abstract: Aflatoxins (AFs) are secondary metabolites produced by different species of Aspergillus, such as Aspergillus ﬂavus and Aspergillus parasiticus, which possess mutagenic, teratogenic and carcinogenic activities in humans. In this study, active packaging devices containing allyl isothiocyanate (AITC) or oriental mustard flour (OMF) + water were tested to inhibit the growth of A. parasiticus and AFs production in fresh pizza crust after 30 d. The antimicrobial and anti-aflatoxin activities were compared to a control group (no antimicrobial treatment) and to a group added with commercial preservatives (sorbic acid + sodium propionate). A. parasiticus growth was only inhibited after 30 d by AITC in filter paper at 5 μL/L and 10 μL/L, AITC sachet at 5 μL/L and 10 μL/L and OMF sachet at 850 mg + 850 μL of water. However, AFs production was inhibited by all antimicrobial treatments in a dose-dependent manner. More importantly, AITC in a filter paper at 10 μL/L, AITC sachet at 10 μL/L, OMF sachet at 850 mg + 850 μL of water and sorbic acid + sodium propionate at 0.5-2.0 g/Kg completely inhibited AFs formation. The use of AITC in active packaging devices could be a natural alternative to avoid the growth of mycotoxinogenic fungi in refrigerated bakery products in substitution of common commercial preservatives.
authors.affiliation: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Quiles
authors.affiliation: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: Manyes
authors.affiliation: School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica, BR 376 Km 14, São José dos Pinhais, PR, 83010-500, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Luciano
authors.affiliation: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Mañes
authors.affiliation: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain. Electronic address: giuseppe.meca@uv.es.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Meca
mesh_terms: D000348:Aflatoxins / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity; D000890:Anti-Infective Agents / Q000378:metabolism*; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D001939:Bread / Q000032:analysis / Q000382:microbiology*; D002273:Carcinogens / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D015169:Colony Count, Microbial; D005506:Food Contamination / Q000517:prevention & control*; D005511:Food Handling; D018857:Food Packaging; D005520:Food Preservatives / Q000378:metabolism*; D017879:Isothiocyanates / Q000378:metabolism*; D009149:Mustard Plant / Q000737:chemistry; D009153:Mutagens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D009822:Oils, Volatile / Q000378:metabolism; D028321:Plant Preparations / Q000378:metabolism; D012034:Refrigeration; D012639:Seeds / Q000737:chemistry; D013030:Spain; D017365:Spices / Q000032:analysis; D013723:Teratogens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity
chemical_list: D000348:Aflatoxins; D000890:Anti-Infective Agents; D002273:Carcinogens; D005520:Food Preservatives; D017879:Isothiocyanates; D009153:Mutagens; D009822:Oils, Volatile; D028321:Plant Preparations; D013723:Teratogens; C004471:allyl isothiocyanate
keywords: Aflatoxins; Antifungal activity; Aspergillus parasiticus; Fresh pizza crust; Mustard essential oil; Sinigrin