title: Fe65 Suppresses Breast Cancer Cell Migration and Invasion through Tip60 Mediated Cortactin Acetylation.
abstract: Fe65 is a brain-enriched adaptor protein known for its role in the action of the Aβ amyloid precursor protein in neuronal cells and Alzheimer's disease, but little is known about its functions in cancer cells. The present study documents for the first time a role of Fe65 in suppressing breast cancer cell migration and invasion. Mechanistic studies suggest that the suppression is mediated through its phosphotyrosine binding domain 1 that mediates the recruitment of Tip60 to cortactin to stimulate its acetylation. The studies identify the Tip60 acetyltransferase as a cytoplasmic drug target for the therapeutic intervention of metastatic breast cancers.
authors.affiliation: Departments of Pathology and Cell Biology, University of South Florida Morsani College of Medicine, Tampa, FL 33612.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Comprehensive Melanoma Research Center and Department of Tumor Biology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Sun
authors.affiliation: Departments of Pathology and Cell Biology, University of South Florida Morsani College of Medicine, Tampa, FL 33612.
authors.forename: Panida
authors.initials: P
authors.lastname: Lungchukiet
authors.affiliation: Departments of Pathology and Cell Biology, University of South Florida Morsani College of Medicine, Tampa, FL 33612.
authors.forename: Waise
authors.initials: W
authors.lastname: Quarni
authors.affiliation: Comprehensive Melanoma Research Center and Department of Tumor Biology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Yang
authors.affiliation: 1] Departments of Pathology and Cell Biology, University of South Florida Morsani College of Medicine, Tampa, FL 33612 [2] Department of Oncological Sciences, University of South Florida Morsani College of Medicine, Tampa, FL 33612 [3] Program of Cancer Biology and Evolution, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1] Departments of Pathology and Cell Biology, University of South Florida Morsani College of Medicine, Tampa, FL 33612 [2] Department of Oncological Sciences, University of South Florida Morsani College of Medicine, Tampa, FL 33612 [3] Program of Cancer Biology and Evolution, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Bai
mesh_terms: D000107:Acetylation; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D051356:Cortactin / Q000378:metabolism*; D005260:Female; D057809:HEK293 Cells; D051548:Histone Acetyltransferases / Q000378:metabolism*; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D000074443:Lysine Acetyltransferase 5; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: C091042:APBB1 protein, human; D051356:Cortactin; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D051548:Histone Acetyltransferases; C098489:KAT5 protein, human; D000074443:Lysine Acetyltransferase 5; C433811:HDAC6 protein, human; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases