title: Identification of dopamine- and serotonin-related genes modulated by bisphenol A in the prefrontal cortex of male rats.
abstract: There is concern that exposure of embryos and/or infants to bisphenol A (BPA) may lead to neurological and behavioral disorders with unknown prefrontal cortex (PFC) involvement. Critical PFC functions are modulated by dopamine (DA) and serotonin (5-HT) systems, whose alterations have been associated with psychopathologies that may appear in youth and/or adulthood. This study aims to determine in the PFC of male rats exposed to a low dose of BPA (10μgkg(-1)d(-1)) from gestational day 12 (GD12) to postnatal day 21 (PND21): (i) DA- and 5-HT-related genes modulated by BPA at the juvenile stage (PND21); (ii) reversible and irreversible transcriptional effects; (iii) long-term consequences (effects in adult rats, PND90). In juvenile rats, BPA altered significantly the transcription of 12 out of the 84 genes analyzed using PCR-array techniques. Interestingly, transcript levels of the neurotrophic factor Gdnf were decrease by BPA in both juvenile and adult rats. At adulthood, disruptions in genes encoding rate-limiting enzymes for DA and 5-HT synthesis emerged. Overall, the results indicate that early-life exposure to BPA has consequences on DA and 5-HT systems in both juvenile- and adult-life stages. Additionally, we reveal molecular targets that could provide the foundation for future BPA neurotoxicity studies.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Granada, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Castro
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Granada, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Sánchez
authors.affiliation: Department of Biostatistics, Faculty of Medicine, University of Granada, Granada, Spain.
authors.forename: María T
authors.initials: MT
authors.lastname: Miranda
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Granada, Spain; Institute of Neurosciences, University of Granada, Granada, Spain. Electronic address: torrespi@ugr.es.
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Torres
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Granada, Spain; Institute of Neurosciences, University of Granada, Granada, Spain. Electronic address: esortega@ugr.es.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Ortega
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001559:Benzhydryl Compounds / Q000633:toxicity*; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics; D008297:Male; D008431:Maternal-Fetal Exchange; D010636:Phenols / Q000633:toxicity*; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004298:Dopamine; D051100:Glial Cell Line-Derived Neurotrophic Factor; D010636:Phenols; D012701:Serotonin; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Cortex; Dopamine; PCR-array; Rat; Serotonin