title: A ratiometric fluorescent system for carboxylesterase detection with AIE dots as FRET donors.
abstract: A ratiometric fluorescent system for CaE detection with AIE dots as the FRET donors was designed. Upon enzymatic reaction, electrostatic interaction between the cationic TPE-N(+) dots and the enzymatic reaction product - the negatively charged fluorescein molecules - allows the FRET process to proceed, thus affording the ratiometric fluorescence CaE assay.
authors.affiliation: College of Materials Science and Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, P. R. China. mcfzeng@scut.edu.cn shzhwu@scut.edu.cn.
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Wu
authors.forename: Shuailing
authors.initials: S
authors.lastname: Huang
authors.forename: Fang
authors.initials: F
authors.lastname: Zeng
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Changmin
authors.initials: C
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Huiting
authors.initials: H
authors.lastname: Xie
authors.forename: Shuizhu
authors.initials: S
authors.lastname: Wu
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D055672:Static Electricity
chemical_list: D005456:Fluorescent Dyes; D002265:Carboxylic Ester Hydrolases; D019793:Fluorescein