title: Integrin LFA-1 regulates cell adhesion via transient clutch formation.
abstract: Integrin LFA-1 regulates immune cell adhesion and trafficking by binding to ICAM-1 upon chemokine stimulation. Integrin-mediated clutch formation between extracellular ICAM-1 and the intracellular actin cytoskeleton is important for cell adhesion. We applied single-molecule tracking analysis to LFA-1 and ICAM-1 in living cells to examine the ligand-binding kinetics and mobility of the molecular clutch under chemokine-induced physiological adhesion and Mn(2+)-induced tight adhesion. Our results show a transient LFA-1-mediated clutch formation that lasts a few seconds and leads to a transient lower-mobility is sufficient to promote cell adhesion. Stable clutch formation was observed for Mn(2+)-induced high affinity LFA-1, but was not required for physiological adhesion. We propose that fast cycling of the clutch formation by intermediate-affinity integrin enables dynamic cell adhesion and migration.
authors.affiliation: Laboratory for Cell Signaling Dynamics, QBiC (Quantitative Biology Center), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Munenori
authors.initials: M
authors.lastname: Ishibashi
authors.affiliation: Laboratory for Cell Signaling Dynamics, QBiC (Quantitative Biology Center), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Miyanaga
authors.affiliation: Laboratory for Cell Signaling Dynamics, QBiC (Quantitative Biology Center), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Matsuoka
authors.affiliation: Laboratory for Cell Signaling Dynamics, QBiC (Quantitative Biology Center), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kozuka
authors.affiliation: Research Center for the Mathematics on Chromatin Live Dynamics, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Togashi
authors.affiliation: Department of Molecular Genetics, Institute of Biomedical Science, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka 573-1010, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kinashi
authors.affiliation: Laboratory for Cell Signaling Dynamics, QBiC (Quantitative Biology Center), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. Electronic address: ueda@bio.sci.osaka-u.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ueda
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007700:Kinetics; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism / Q000502:physiology*
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1
keywords: Cell adhesion; Chemokine; Immune cell; Integrin; Single-molecule