title: Proteomic analysis of Arabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer).
abstract: Herbivorous insects can cause severe cellular changes to plant foliage following infestations, depending on feeding behaviour. Here, a proteomic study was conducted to investigate the influence of green peach aphid (Myzus persicae Sulzer) as a polyphagous pest on the defence response of Arabidopsis thaliana (L.) Heynh after aphid colony establishment on the host plant (3 days). Analysis of about 574 protein spots on 2-DE gels revealed 31 differentially expressed protein spots. Twenty out of these 31 differential proteins were selected for analysis by mass spectrometry. In 12 of the 20 analysed spots, we identified seven and nine proteins using MALDI-TOF-MS and LC-ESI-MS/MS, respectively. Of the analysed spots, 25% contain two proteins. Different metabolic pathways were modulated in Arabidopsis leaves according to aphid feeding: most corresponded to carbohydrate, amino acid and energy metabolism, photosynthesis, defence response and translation. This paper has established a survey of early alterations induced in the proteome of Arabidopsis by M. persicae aphids. It provides valuable insights into the complex responses of plants to biological stress, particularly for herbivorous insects with sucking feeding behaviour.
authors.affiliation: Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
authors.forename: D-H
authors.initials: DH
authors.lastname: Truong
authors.affiliation: Functional & Evolutionary Entomology, University of Liège, Gembloux Agro-Bio Tech, Gembloux, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Bauwens
authors.affiliation: Plant Biology, University of Liège, Gembloux Agro-Bio Tech, Gembloux, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Delaplace
authors.affiliation: Mass Spectrometry Laboratory, University of Liège, Liège, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Mazzucchelli
authors.affiliation: Analytical Chemistry Laboratory, University of Liège, Gembloux Agro-Bio Tech, Gembloux, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Lognay
authors.affiliation: Functional & Evolutionary Entomology, University of Liège, Gembloux Agro-Bio Tech, Gembloux, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Francis
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001042:Aphids; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D060434:Herbivory; D010935:Plant Diseases; D018515:Plant Leaves; D020543:Proteome; D040901:Proteomics; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome
keywords: 2-DE; Arabidopsis thaliana; LC-ESI-MS/MS; MALDI-TOF MS; Myzus persicae; proteomic expression