title: The promotion of salinomycin delivery to hepatocellular carcinoma cells through EGFR and CD133 aptamers conjugation by PLGA nanoparticles.
abstract: OBJECTIVE
  To develop salinomycin-loaded poly(lactic-co-glycolic acid) nanoparticles conjugated with both CD133 aptamers A15 and EGFR aptamers CL4 (CESN), to target hepatocellular carcinoma (HCC) cells simultaneously expressing EGFR and CD133.
  
  
  METHODS
  The antitumor activity and mechanism of CESN were investigated.
  
  
  CONCLUSIONS
  The cytotoxicity of CESN in HCC cells and CD133(+) HCC cells was superior to that of A15 or CL4-conjugted or nontargeted salinomycin-loaded nanoparticles. The antitumor assay in mice bearing HCC xenograft tumors confirmed the superior antitumor activity of CESN over other controls. We speculated that the improved therapeutic effect of CESN may be attributed to both targeting a higher percentage of HCC cells and increased delivery of salinomycin to HCC cells.
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Road, Guizhou 550001, Guiyang, Guizhou, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Road, Guizhou 550001, Guiyang, Guizhou, China.|International Join Cancer Institute, The Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Huaiwen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Road, Guizhou 550001, Guiyang, Guizhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: College of Pharmacy, The Second Military Medical University, 325 Guo He Road, Shanghai 200433, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Road, Guizhou 550001, Guiyang, Guizhou, China.
authors.forename: Meiyuan
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Road, Guizhou 550001, Guiyang, Guizhou, China.
authors.forename: She
authors.initials: S
authors.lastname: Tian
authors.affiliation: Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Road, Guizhou 550001, Guiyang, Guizhou, China.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Sun
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics*; D000970:Antineoplastic Agents / Q000008:administration & dosage; D052157:Aptamers, Nucleotide / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D066246:ErbB Receptors / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D058990:Molecular Targeted Therapy / Q000379:methods; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D010455:Peptides / Q000235:genetics*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011714:Pyrans / Q000008:administration & dosage* / Q000737:chemistry; D016896:Treatment Outcome; D000095662:Polyether Polyketides
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D066246:ErbB Receptors; D006023:Glycoproteins; D019344:Lactic Acid; D053769:Nanocapsules; D058726:Nanoconjugates; D010455:Peptides; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011714:Pyrans; C000606023:PROM1 protein, human; C000606024:Prom1 protein, mouse; C010327:salinomycin; D000095662:Polyether Polyketides; C512478:EGFR protein, human
keywords: CD133; EGFR; hepatocellular carcinoma; nanoparticles; salinomycin