title: A water-soluble pH fluorescence probe based on quaternary ammonium salt for bioanalytical applications.
abstract: A novel fluorescence probe Rhodamine-Ethanediamine-Iodomethane (REI) was successfully prepared to serve as an efficient sensing platform for H(+) with fully reversibility mainly between the pH 4.2 and 7.2 in simple buffer solution. The introduction of quaternary ammonium salt with positive charge can not only manage to increase the solubility and sensitivity of probe REI, but also avoid the "alkalizing effect" due to charge-induced effect compared to the reference probe Rhodamine-Ethanediamine (RE). In particular, probe REI was well used for monitoring the weak acid pH fluctuations in lysosome of the live HeLa cells due to its excellent biological properties, including low cytotoxicity, high selectivity, good sensitivity and membrane permeability.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Xuan-Xuan
authors.initials: XX
authors.lastname: Zhao
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China; School of Biological Science and Technology, University of Jinan, Jinan 250022, PR China. Electronic address: gedi_blue@126.com.
authors.forename: Di
authors.initials: D
authors.lastname: Ge
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Dai
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Wu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D000644:Quaternary Ammonium Compounds; D014867:Water
keywords: Alkalizing effect; Fluorescence probe; H(+); Lysosome; Quaternary ammonium salt