title: Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice.
abstract: Rice (Oryza sativa L.) production, essential for global food security, is threatened by the brown planthopper (BPH). The breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. An indica rice introgression line RBPH54, derived from wild rice Oryza rufipogon, has been identified with sustainable resistance to BPH, which is governed by recessive alleles at two loci. In this study, a map-based cloning approach was used to fine-map one resistance gene locus to a 24kb region on the short arm of chromosome 6. Through genetic analysis and transgenic experiments, BPH29, a resistance gene containing a B3 DNA-binding domain, was cloned. The tissue specificity of BPH29 is restricted to vascular tissue, the location of BPH attack. In response to BPH infestation, RBPH54 activates the salicylic acid signalling pathway and suppresses the jasmonic acid/ethylene-dependent pathway, similar to plant defence responses to biotrophic pathogens. The cloning and characterization of BPH29 provides insights into molecular mechanisms of plant-insect interactions and should facilitate the breeding of rice host-resistant varieties.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and College of Agriculture, Guangxi University, Nanning 530004, China.
authors.forename: Yuexiong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Changxiang
authors.initials: C
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and College of Agriculture, Guangxi University, Nanning 530004, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
authors.forename: Fengkuan
authors.initials: F
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and College of Agriculture, Guangxi University, Nanning 530004, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and College of Agriculture, Guangxi University, Nanning 530004, China luoxj@fudan.edu.cn lirongbai@126.com.
authors.forename: Rongbai
authors.initials: R
authors.lastname: Li
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China luoxj@fudan.edu.cn lirongbai@126.com.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Lou
mesh_terms: D000818:Animals; D000898:Antibiosis; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005808:Genes, Recessive; D006430:Hemiptera / Q000502:physiology*; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D057865:Plant Immunity; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000276:immunology / Q000502:physiology
chemical_list: D010940:Plant Proteins
keywords: B3 domain protein; BPH29; Oryza sativa L.; brown planthopper resistance; map-based cloning; plant–insect defence; recessive gene.