title: Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
abstract: Drug resistance is a global threat and one of the main contributing factors to tuberculosis (TB) outbreaks. The goal of this study was to analyse the molecular profile of multidrug-resistant TB (MDR-TB) in the state of Santa Catarina in southern Brazil. Fifty-three MDR Mycobacterium tuberculosis clinical isolates were analysed by spoligotyping and a partial region of the rpoB gene, which is associated with rifampicin resistance (RMP-R), was sequenced. Some isolates were also distinguished by their mycobacterial interspersed repetitive units (MIRU). S531L was the most prevalent mutation found within rpoB in RMP-R isolates (58.5%), followed by S531W (20.8%). Only two MDR isolates showed no mutations within rpoB. Isolates of the Latin American Mediterranean (LAM) family were the most prevalent (45.3%) found by spoligotyping, followed by Haarlem (9.4%) and T (7.5%) families. SIT106 was found in 26.4% of isolates and all SIT106 isolates typed by MIRU-12 (5 out of 14) belong to MIT251. There was a high correlation between the S531W mutation and the LAM family mainly because all SIT2263 (LAM9) isolates carry this mutation. Among isolates with the S531W mutation in rpoB MIRU demonstrates a cluster formed by four isolates (SIT2263 and MIT163) and very similar profiles were observed between eight of the nine isolates. Better characterisation of TB isolates may lead to new ways in which to control and treat TB in this region of Brazil.
authors.affiliation: Laboratório de Biologia Molecular, Sorologia e Micobactérias, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
authors.forename: Rodrigo Ivan
authors.initials: RI
authors.lastname: Prim
authors.affiliation: Laboratório de Biologia Molecular, Sorologia e Micobactérias, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
authors.forename: Marcos André
authors.initials: MA
authors.lastname: Schörner
authors.affiliation: Laboratório de Biologia Molecular, Sorologia e Micobactérias, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
authors.forename: Simone Gonçalves
authors.initials: SG
authors.lastname: Senna
authors.affiliation: Laboratório de Biologia Molecular, Sorologia e Micobactérias, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
authors.forename: Christiane Lourenço
authors.initials: CL
authors.lastname: Nogueira
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, BR.
authors.forename: Anna Carolina Cançado
authors.initials: AC
authors.lastname: Figueiredo
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, BR.
authors.forename: Jaquelline Germano de
authors.initials: JG
authors.lastname: Oliveira
authors.affiliation: Laboratório Central de Saúde Pública de Santa Catarina, Florianópolis, SC, BR.
authors.forename: Darcita Bürger
authors.initials: DB
authors.lastname: Rovaris
authors.affiliation: Laboratório de Biologia Molecular, Sorologia e Micobactérias, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Bazzo
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001938:Brazil; D004269:DNA, Bacterial; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial