title: Identification of proteins involved in desiccation tolerance in the red seaweed Pyropia orbicularis (Rhodophyta, Bangiales).
abstract: Extreme reduction in cellular water content leads to desiccation, which, if persistent, affects the physiology of organisms, mainly through oxidative stress. Some organisms are highly tolerant to desiccation, including resurrection plants and certain intertidal seaweeds. One such species is Pyropia orbicularis, a rhodophycean that colonizes upper intertidal zones along the Chilean coast. Despite long, daily periods of air exposure due to tides, this alga is highly tolerant to desiccation. The present study examined the proteome of P. orbicularis by 2DE and LC-MS/MS analyses to determine the proteins associated with desiccation tolerance (DT). The results showed that, under natural conditions, there were significant changes in the protein profile during low tide as compared to naturally hydrated plants at high tide. These changes were mainly in newly appeared proteins spots such as chaperones, monodehydroascorbate reductase, and manganese superoxide dismutase, among others. Previously undescribed proteins under desiccation conditions included phycobiliproteins, glyoxalase I, and phosphomannomutase. These changes evidenced that several physiological responses involved in DT are activated during low tide, including decreased photosynthetic activity, increased antioxidant capacity, and the preservation of cell physiology by regulating water content, cell wall structure, and cell volume. Similar responses have been observed in resurrection plants and bryophytes exposed to desiccation. Therefore, the coordinated activation of different desiccation tolerance pathways in P. orbicularis could explain the successful biological performance of this seaweed in the upper intertidal rocky zones.
authors.affiliation: Departamento de Ecología y Biodiversidad, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Santiago, Chile.|Departament de Biologia Vegetal, Universitat de Barcelona, Barcelona, España.
authors.forename: Camilo
authors.initials: C
authors.lastname: López-Cristoffanini
authors.affiliation: Departamento de Ecología y Biodiversidad, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Santiago, Chile.
authors.forename: Javier
authors.initials: J
authors.lastname: Zapata
authors.affiliation: Centre de Ressources de Biologie Marine, MetaboMer Mass Spectrometry Core Facility, CNRS, Université Pierre et Marie Curie, Roscoff cedex, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Gaillard
authors.affiliation: Sorbonne Universités, CNRS, Université Pierre et Marie Curie UMR 8227, Station Biologique, Roscoff, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Potin
authors.affiliation: Departamento de Ecología, and Center of Applied Ecology and Sustainability (CAPES), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile and UMI 3614, Evolutionary Biology and Ecology of Algae, Station Biologique de Roscoff, France.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Correa
authors.affiliation: Departamento de Ecología y Biodiversidad, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Santiago, Chile.|Center of Applied Ecology and Sustainability (CAPES), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Loretto
authors.initials: L
authors.lastname: Contreras-Porcia
mesh_terms: D000222:Adaptation, Physiological; D003890:Desiccation; D018384:Oxidative Stress / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D000461:Rhodophyta / Q000378:metabolism / Q000502:physiology; D012624:Seaweed / Q000378:metabolism* / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D010940:Plant Proteins
keywords: Desiccation tolerance; Oxidative stress; Pyropia; Seaweeds