title: Supported Molecular Matrix Electrophoresis.
abstract: Mucins are difficult to separate using conventional gel electrophoresis methods such as SDS-PAGE and agarose gel electrophoresis, owing to their large size and heterogeneity. On the other hand, cellulose acetate membrane electrophoresis can separate these molecules, but is not compatible with glycan analysis. Here, we describe a novel membrane electrophoresis technique, termed "supported molecular matrix electrophoresis" (SMME), in which a porous polyvinylidene difluoride (PVDF) membrane filter is used to achieve separation. This description includes the separation, visualization, and glycan analysis of mucins with the SMME technique.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), OSL, Central2, 1-1-1 Umesono, Tsukuba, 305-8568, Japan.
authors.forename: Yu-Ki
authors.initials: YK
authors.lastname: Matsuno
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kameyama
mesh_terms: D000423:Alcian Blue / Q000737:chemistry; D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D008567:Membranes, Artificial; D009077:Mucins / Q000032:analysis* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D011145:Polyvinyls / Q000737:chemistry*; D013552:Swine; D005465:Fluorocarbon Polymers
chemical_list: D000423:Alcian Blue; D004396:Coloring Agents; D008567:Membranes, Artificial; D009077:Mucins; D011134:Polysaccharides; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers