title: Confirmation of delayed canopy wilting QTLs from multiple soybean mapping populations.
abstract: QTLs for delayed canopy wilting from five soybean populations were projected onto the consensus map to identify eight QTL clusters that had QTLs from at least two independent populations. Quantitative trait loci (QTLs) for canopy wilting were identified in five recombinant inbred line (RIL) populations, 93705 KS4895 × Jackson, 08705 KS4895 × Jackson, KS4895 × PI 424140, A5959 × PI 416937, and Benning × PI 416937 in a total of 15 site-years. For most environments, heritability of canopy wilting ranged from 0.65 to 0.85 but was somewhat lower when averaged over environments. Putative QTLs were identified with composite interval mapping and/or multiple interval mapping methods in each population and positioned on the consensus map along with their 95% confidence intervals (CIs). We initially found nine QTL clusters with overlapping CIs on Gm02, Gm05, Gm11, Gm14, Gm17, and Gm19 identified from at least two different populations, but a simulation study indicated that the QTLs on Gm14 could be false positives. A QTL on Gm08 in the 93705 KS4895 × Jackson population co-segregated with a QTL for wilting published previously in a Kefeng1 × Nannong 1138-2 population, indicating that this may be an additional QTL cluster. Excluding the QTL cluster on Gm14, results of the simulation study indicated that the eight remaining QTL clusters and the QTL on Gm08 appeared to be authentic QTLs. QTL × year interactions indicated that QTLs were stable over years except for major QTLs on Gm11 and Gm19. The stability of QTLs located on seven clusters indicates that they are possible candidates for use in marker-assisted selection.
authors.affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 Altheimer Drive, Fayetteville, AR, 72704, USA.
authors.forename: Sadal
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 Altheimer Drive, Fayetteville, AR, 72704, USA.
authors.forename: C Andy
authors.initials: CA
authors.lastname: King
authors.affiliation: Crop Genetics and Production Research Unit, USDA-ARS, Stoneville, MS, 38776, USA.
authors.forename: Jeffery D
authors.initials: JD
authors.lastname: Ray
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARR, BARC-West, Beltsville, MD, 20705-2350, USA.
authors.forename: Perry B
authors.initials: PB
authors.lastname: Cregan
authors.affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 Altheimer Drive, Fayetteville, AR, 72704, USA.
authors.forename: Pengyin
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Crop Science, North Carolina State University, USDA-ARS, Raleigh, NC, 27695, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Carter
authors.affiliation: Department of Crop and Soil Sciences and Center for Applied Genetic Technologies, The University of Georgia, 111 Riverbend Rd., Athens, GA, 30602-6810, USA.
authors.forename: Zenglu
authors.initials: Z
authors.lastname: Li
authors.affiliation: US Arid-Land Agricultural Research Center, USDA-ARS, 21881 North Cardon Lane, Maricopa, AZ, 85138, USA.
authors.forename: Hussein
authors.initials: H
authors.lastname: Abdel-Haleem
authors.affiliation: Global Soybean Breeding, Monsanto Company, St. Louis, MO, 63167, USA.
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Matson
authors.affiliation: Department of Agronomy, Kansas State University, 2004C Throckmorton Hall, Manhattan, KS, 6506-5501, USA.
authors.forename: William
authors.initials: W
authors.lastname: Schapaugh
authors.affiliation: Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 Altheimer Drive, Fayetteville, AR, 72704, USA. lpurcell@uark.edu.
authors.forename: Larry C
authors.initials: LC
authors.lastname: Purcell
mesh_terms: D002874:Chromosome Mapping; D055864:Droughts; D004777:Environment; D005828:Genetics, Population; D005838:Genotype; D010641:Phenotype; D000069600:Plant Breeding; D040641:Quantitative Trait Loci; D013025:Glycine max / Q000235:genetics* / Q000502:physiology*