title: Synthesis of self-reporting polymeric nanoparticles for in situ monitoring of endocytic microenvironmental pH.
abstract: The bis(pyrene) conjugated pH-sensitive polymers () were synthesized and self-assembled into nanoparticles through hydrophobic interactions. The Nile red (NR) loaded nanoparticles showed red emission due to the FRET effect. The nanoparticles entered cells via endocytosis, and the microenvironmental pH in the endocytosis process was monitored in situ by the simultaneous dual-wavelength fluorescence changes.
authors.affiliation: CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), Beijing 100190, China. wanghao@nanoctr.cn wanglei@nanoctr.cn.
authors.forename: Zeng-Ying
authors.initials: ZY
authors.lastname: Qiao
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Hou
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Zhao
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry
chemical_list: D010078:Oxazines; D011108:Polymers; D011721:Pyrenes; C030984:pyrene; C044808:nile red