title: Carbon Quantum Dots for Zebrafish Fluorescence Imaging.
abstract: Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Kang
authors.affiliation: Tianjin Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, School of Medicine, Nankai University, Tianjin, 300071, China.
authors.forename: Yu-Hao
authors.initials: YH
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, School of Medicine, Nankai University, Tianjin, 300071, China.
authors.forename: Yang-Wu
authors.initials: YW
authors.lastname: Fang
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Xiao-Mi
authors.initials: XM
authors.lastname: Wei
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Yin
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000166:cytology; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D008297:Male; D008845:Microinjections; D008856:Microscopy, Fluorescence; D045663:Quantum Dots / Q000737:chemistry*; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D002244:Carbon