title: Fibre-coupled multiphoton microscope with adaptive motion compensation.
abstract: To address the challenge of sample motion during in vivo imaging, we present a fibre-coupled multiphoton microscope with active axial motion compensation. The position of the sample surface is measured using optical coherence tomography and fed back to a piezo actuator that adjusts the axial location of the objective to compensate for sample motion. We characterise the system's performance and demonstrate that it can compensate for axial sample velocities up to 700 µm/s. Finally we illustrate the impact of motion compensation when imaging multiphoton excited autofluorescence in ex vivo mouse skin.
authors.affiliation: Department of Physics, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Ben
authors.initials: B
authors.lastname: Sherlock
authors.affiliation: Department of Physics, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Sean
authors.initials: S
authors.lastname: Warren
authors.affiliation: Department of Physics, University of Bath, Bath, BA2 7AY, UK.
authors.forename: James
authors.initials: J
authors.lastname: Stone
authors.affiliation: Department of Physics, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Neil
authors.affiliation: Department of Physics, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Carl
authors.initials: C
authors.lastname: Paterson
authors.affiliation: Department of Physics, University of Bath, Bath, BA2 7AY, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Knight
authors.affiliation: Department of Physics, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: French
authors.affiliation: Department of Physics, Imperial College London, London, SW7 2AZ, UK ; Centre for Histopathology, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Dunsby
keywords: (180.4315) Nonlinear microscopy; (330.4150) Motion detection