title: Generation of recombinant bioluminescent Escherichia coli for quantitative determination of bacterial adhesion.
abstract: Bacterial adhesion to urinary catheter was evaluated by measuring the light emitted from a recombinant bioluminescent glycocalyx producer Escherichia coli strain. Generation of the bioluminescent strain was carried out by transforming the bacterial cells with pUCP18-GFP plasmid that contains a green fluorescence gene. Light emission measurement was closely correlated with the number of the adherent cells, giving a detectable signal from 1.2 X 10² cells. The efficiency of this assay was confirmed by testing the antiadherent effect of subinhibitory concentrations of ciprofloxacin with the aid of a model for in-vitro catheter colonization. There was no significant difference in the percentage reduction of adherent cells obtained by both light emission measurement and viable cell count techniques.
authors.affiliation: Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, KSA.
authors.forename: Khalid Abdul Rahman
authors.initials: KA
authors.lastname: AlLuhaybi
authors.affiliation: Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, KSA.
authors.forename: Ghadah Yazeed
authors.initials: GY
authors.lastname: Alghaith
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Giza, Cairo, Egypt.
authors.forename: Nayera Ahmed
authors.initials: NA
authors.lastname: Moneib
authors.affiliation: Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, KSA.
authors.forename: Mahmoud Abdul Magead
authors.initials: MA
authors.lastname: Yassien
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D002939:Ciprofloxacin / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology*; D019276:Glycocalyx / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D008163:Luminescent Measurements
chemical_list: D049452:Green Fluorescent Proteins; D002939:Ciprofloxacin