title: Enhanced effect of photodynamic therapy in ovarian cancer using a nanoparticle drug delivery system.
abstract: Nanoparticles are promising novel drug delivery carriers that allow tumor targeting and controlled drug release. In the present study, we prepared poly butyl-cyanoacrylate nanoparticles (PBCA-NP) entrapped with hypocrellin B (HB) to improve the effect of photodynamic therapy (PDT) in ovarian cancer. An ovarian cancer ascites model using Fischer 344 rats and PBCA-NP entrapped with HB (HB-PBCA-NP) were formed successfully. The pharmacodynamic characteristics and biodistribution of the HB-PBCA-NP system were evaluated by comparison with HB dimethyl sulfoxide (HB-DMSO) and testing at various time-points following intraperitoneal drug administration. HB-PBCA-NP-based PDT combined with cytoreductive surgery was then administrated to the tumor-bearing animals. Kaplan-Meier survival analysis was performed to assess the therapeutic effect of the nanoparticle system. The serum HB concentration peaked 4 h after drug administration in the nanoparticle system, and 1 h with HB-DMSO. The peak exposure time of tumor tissues was also extended (4 vs. 2 h), and PBCA-NP remained present for much longer than HB-DMSO. Although PDT combined with surgery prolonged the survival time significantly compared with surgery alone (84 days, P<0.05), there was no significant difference in the survival time of animals that received either HB-PBCA-NP- or HB-DMSO-based PDT after cytoreductive surgery (99 vs. 95 days, P=0.293). PBCA-NP exhibited potential advantages in controlled drug release and tumor targeting, which was beneficial for HB-based PDT. PDT combined with surgery prolonged the survival time, suggesting that this might be an alternative treatment option for ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong 250061, P.R. China.
authors.forename: Xuantao
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Basic Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Xun
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Beihua
authors.initials: B
authors.lastname: Kong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004659:Enbucrilate / Q000737:chemistry; D005260:Female; D007274:Injections, Intraperitoneal; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000601:surgery*; D010569:Perylene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011809:Quinones / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051381:Rats; D014018:Tissue Distribution; D016896:Treatment Outcome
chemical_list: D017319:Photosensitizing Agents; D011809:Quinones; D010569:Perylene; D004659:Enbucrilate; C064522:hypocrellin B; D004121:Dimethyl Sulfoxide