title: Fish Oil Accelerates Diet-Induced Entrainment of the Mouse Peripheral Clock via GPR120.
abstract: The circadian peripheral clock is entrained by restricted feeding (RF) at a fixed time of day, and insulin secretion regulates RF-induced entrainment of the peripheral clock in mice. Thus, carbohydrate-rich food may be ideal for facilitating RF-induced entrainment, although the role of dietary oils in insulin secretion and RF-induced entrainment has not been described. The soybean oil component of standard mouse chow was substituted with fish or soybean oil containing docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA). Tuna oil (high DHA/EPA), menhaden oil (standard), and DHA/EPA dissolved in soybean oil increased insulin secretion and facilitated RF-induced phase shifts of the liver clock as represented by the bioluminescence rhythms of PER2::LUCIFERASE knock-in mice. In this model, insulin depletion blocked the effect of tuna oil and fish oil had no effect on mice deficient for GPR120, a polyunsaturated fatty acid receptor. These results suggest food containing fish oil or DHA/EPA is ideal for adjusting the peripheral clock.
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Furutani
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ikeda
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Misa
authors.initials: M
authors.lastname: Itokawa
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nagahama
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Teiji
authors.initials: T
authors.lastname: Ohtsu
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Furutani
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Mayo
authors.initials: M
authors.lastname: Kamagata
authors.affiliation: Central Research Laboratory, Nippon Suisan Kaisha Ltd., Nanakuni 1-32-3, Hachioji, Tokyo, Japan.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yang
authors.affiliation: Department of Genomic Drug Discovery Sciences, Kyoto University, 46-29, Yoshida, Sakyo-ku, Kyoto, Japan; Institute for Integrated Medical Sciences, Tokyo Women's Medical University, Kawada-cho 8-1, Shinjuku-ku, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hirasawa
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tahara
authors.affiliation: Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Wakamatsu-cho 2-2, Shinjuku-ku, Tokyo, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Shibata
mesh_terms: D000284:Administration, Oral; D000818:Animals; D057906:Circadian Clocks / Q000187:drug effects* / Q000235:genetics; D004032:Diet; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007267:Injections; D007328:Insulin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency / Q000378:metabolism*; D013024:Soybean Oil / Q000494:pharmacology; D013311:Streptozocin; D013493:Suprachiasmatic Nucleus / Q000187:drug effects / Q000502:physiology
chemical_list: C498007:FFAR4 protein, mouse; D005395:Fish Oils; D007328:Insulin; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D004281:Docosahexaenoic Acids; D013311:Streptozocin; D013024:Soybean Oil; D015118:Eicosapentaenoic Acid