title: Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells.
abstract: Arrays of protein nanodots with dot-size tuned independently of spacing (e.g., ∼100 to 600 nm diameter for 900 nm spacing) are fabricated. The mechanism of size control is demonstrated, by numerical simulations, to arise from shadow effects during deposition of a sacrificial metal mask. We functionalize the nanodots with antibodies and embed them in a polymer-cushion or in lipid-bilayers or transfer them to soft elastomers. Their ability to influence cell architecture and local membrane organization is demonstrated in T-lymphocytes, using reflection interference contrast and total internal reflection fluorescence microscopy.
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Pi
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.|‡Laboratoire Adhésion and Inflammation, Aix-Marseille Université/Inserm U1067/CNRS-UMR7333, Marseille13288, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Dillard
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Ranime
authors.initials: R
authors.lastname: Alameddine
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Benard
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Astrid
authors.initials: A
authors.lastname: Wahl
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Igor
authors.initials: I
authors.lastname: Ozerov
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Charrier
authors.affiliation: ‡Laboratoire Adhésion and Inflammation, Aix-Marseille Université/Inserm U1067/CNRS-UMR7333, Marseille13288, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Limozin
authors.affiliation: †Aix-Marseille Université, CNRS, CINaM-UMR 7325, Marseille 13288, France.
authors.forename: Kheya
authors.initials: K
authors.lastname: Sengupta
keywords: Nanobiopatterning; T lymphocyte; antibody nanodots; nanosphere lithography