title: [Effect of different dietary fat intake on blood lipids, body fat, adiponectin and leptin on energy balance status in rats].
abstract: OBJECTIVE
  To investigate the effects of different dietary fat intake on body fat, adiponectin and leptin on energy balance status in rats.
  
  
  METHODS
  Forty male SD rats were randomly assigned to four groups. Rats in low fat, normal fat, medium fat and high fat group were fed equal energy diets of low fat diet (5% energy from fat), normal diet (15% energy from fat), medium fat diet (25% energy from fat) and high fat diet (40% energy from fat) respectively. Blood glucose and lipids were analyzed at 0, 5 and 10 weeks. The level of serum adiponectin and leptin was tested at 0 and 10 weeks. At the end of 10 weeks, the rats were sacrificed, the perirenal and periepididymis fat were separated and weighed. The mRNA of adiponectin and leptin in fat tissues were determined by realtime PCR.
  
  
  RESULTS
  After the 5 and 10 weeks, the levels of serum triglyceride of rats in medium fat group and high fat group were lower than those in low fat group and normal fat group. At the end of 10 weeks, the expression of adiponectin mRNA in fat tissues in medium fat group was lower than those in low fat group. There were no significant differences among four groups in body fat, blood glucose, blood cholesterol, serum adiponectin and leptin, and the expression of leptin mRNA in fat tissues.
  
  
  CONCLUSIONS
  In energy balance status, different dietary fat intake had no effects on body fat, blood glucose, blood cholesterol, serum adiponectin and leptin in rats.
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhuo
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Piao
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D001786:Blood Glucose; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D004734:Energy Metabolism; D020738:Leptin / Q000097:blood* / Q000235:genetics; D008055:Lipids / Q000097:blood*; D008297:Male; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D004041:Dietary Fats; D020738:Leptin; D008055:Lipids; D012333:RNA, Messenger