title: Histone H2B Monoubiquitination Mediated by HISTONE MONOUBIQUITINATION1 and HISTONE MONOUBIQUITINATION2 Is Involved in Anther Development by Regulating Tapetum Degradation-Related Genes in Rice.
abstract: Histone H2B monoubiquitination (H2Bub1) is an important regulatory mechanism in eukaryotic gene transcription and is essential for normal plant development. However, the function of H2Bub1 in reproductive development remains elusive. Here, we report rice (Oryza sativa) HISTONE MONOUBIQUITINATION1 (OsHUB1) and OsHUB2, the homologs of Arabidopsis (Arabidopsis thaliana) HUB1 and HUB2 proteins, which function as E3 ligases in H2Bub1, are involved in late anther development in rice. oshub mutants exhibit abnormal tapetum development and aborted pollen in postmeiotic anthers. Knockout of OsHUB1 or OsHUB2 results in the loss of H2Bub1 and a reduction in the levels of dimethylated lysine-4 on histone 3 (H3K4me2). Anther transcriptome analysis revealed that several key tapetum degradation-related genes including OsC4, rice Cysteine Protease1 (OsCP1), and Undeveloped Tapetum1 (UDT1) were down-regulated in the mutants. Further, chromatin immunoprecipitation assays demonstrate that H2Bub1 directly targets OsC4, OsCP1, and UDT1 genes, and enrichment of H2Bub1 and H3K4me2 in the targets is consistent to some degree. Our studies suggest that histone H2B monoubiquitination, mediated by OsHUB1 and OsHUB2, is an important epigenetic modification that in concert with H3K4me2, modulates transcriptional regulation of anther development in rice.
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Meng
authors.initials: M
authors.lastname: Ding
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Fengqin
authors.initials: F
authors.lastname: Dong
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Fengying
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Li'an
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: James
authors.initials: J
authors.lastname: Doughty
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.).
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Molecular Physiology (H.C., X.L., Z.W., M.D., Y.S., F.D., F.C., Y.-X.L.) and Beijing Botanical Garden (L.L.), Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;University of Chinese Academy of Sciences, Beijing 100049, China (X.L., M.D.);Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom (J.D.); andDepartment of Internal Medicine IV, University of Hospital Freiburg, 79106 Freiburg, Germany (Y.L.) yongxiu@ibcas.ac.cn.
authors.forename: Yong-Xiu
authors.initials: YX
authors.lastname: Liu
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics; D044127:Epigenesis, Genetic; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D042421:Histone Code; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D008954:Models, Biological; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; D010940:Plant Proteins; C521255:HUB1 protein, Arabidopsis; C521256:HUB2 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases; D008239:Lysine