title: Propagation of human iPS cells in alginate-based microcapsules prepared using reactions catalyzed by horseradish peroxidase and catalase.
abstract: Cell encapsulation has been investigated as a bioproduction system in the biomedical and pharmaceutical fields. We encaps-ulated human induced pluripotent stem (hiPS) cells in duplex microcapsules prepared from an alginate derivative possessing phenolic hydroxyl moieties, in a single-step procedure based on two competing enzymatic reactions catalyzed by horseradish peroxidase (HRP) and catalase. The encapsulated cells maintained 91.4% viability and proliferated to fill the microcapsules following 19 days of culture. Encapsulated hiPS cells showed pluripotency comparable to that of unencapsulated cells during the cultures, as demonstrated by the expression of the SSEA-4 marker.
authors.affiliation: a Division of Chemical Engineering, Department of Materials Engineering Science , Graduate School of Engineering Science, Osaka University , Toyonaka-shi, Osaka , Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ashida
authors.affiliation: a Division of Chemical Engineering, Department of Materials Engineering Science , Graduate School of Engineering Science, Osaka University , Toyonaka-shi, Osaka , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.affiliation: a Division of Chemical Engineering, Department of Materials Engineering Science , Graduate School of Engineering Science, Osaka University , Toyonaka-shi, Osaka , Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules; D002374:Catalase / Q000737:chemistry*; D002384:Catalysis; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D006735:Horseradish Peroxidase; D002374:Catalase
keywords: bioencapsulation; catalase; duplex microcapsule; enzymatic reactions; horseradish peroxidase; iPS cells; pluripotency; stem cells; tissue engineering