title: [Kinetic Characteristics of Degradation of Geosmin and 2-Methylisoborneol by Bacillus subtilis].
abstract: The earthy and musty odor problem in aquaculture systems has been a worldwide problem, especially in freshwater aquaculture systems. Geosmin (GSM) and 2-methylisoborneol (2-MIB), the most common causative agents of the off-flavor in fish, are lipophilic secondary metabolites of cyanobacteria, actinomycetes, and other microorganisms. The odor threshold concentrations for 2-MIB and GSM are approximately 9-42 ng x L(-1) and 4-10 ng x L(-1), and 600 ng x kg(-1) and 900 ng x kg(-1) in the aquaculture water and fish, respectively. With such a low odor threshold concentration, the off-flavor compounds greatly reduce the quality and economic value of aquatic products. This renders the fish, especially some valuable fish produced in recirculating aquaculture systems (RAS), unmarketable. The study reported the kinetic characteristics of degradation of GSM and 2-MIB by Bacillus subtilis and discussed the impacts of the initial concentration of GSM and 2-MIB (T1, T2) and inoculation amount (T1, T3 ) on the biodegradation rate. The result demonstrated that these two compounds could be degraded by B. subtilis effectively and the biodegradation rate reached more than 90% in T1, T2 and T3 treatments. The biodegradation of these two compounds behaved as a pseudo-first-order kinetics with rate constants (K) in the range of 0.14-0.41. K values indicated that the degradation rate was dependent on the inoculation amount but the start concentration of GSM and MIB. The degradation kinetics showed the maximum specific rate value (u(max)) and the Monod constant (K(s)) were 0.311 and 1.73, however, 2-MIB degradation process did not meet the Monod microbial growth equation (R2 = 0.781).
authors.forename: Nian-nian
authors.initials: NN
authors.lastname: Ma
authors.forename: Guo-zhi
authors.initials: GZ
authors.lastname: Luo
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Tan
authors.forename: Miao-lan
authors.initials: ML
authors.lastname: Yao
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: Wang
mesh_terms: D039903:Actinobacteria; D000818:Animals; D017756:Aquaculture; D001412:Bacillus subtilis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001892:Camphanes / Q000737:chemistry*; D000458:Cyanobacteria; D005399:Fishes; D005618:Fresh Water; D009284:Naphthols / Q000737:chemistry*; D009812:Odorants; D014867:Water
chemical_list: D001892:Camphanes; D009284:Naphthols; D014867:Water; C005536:2-methylisoborneol; C001278:geosmin