title: Uptake of antimonite and antimonate by arsenic hyperaccumulator Pteris vittata: Effects of chemical analogs and transporter inhibitor.
abstract: Antimonite (SbIII) is transported into plants via aquaglyceroporin channels but it is unknown in As-hyperaccumulator Ptreis vittata (PV). We tested the effects of SbIII analogs (arsenite-AsIII, glycerol, silicic acid-Si, and, glucose), antimonate (SbV) analog (phosphate-P), and aquaglyceroporin transporter inhibitor (silver, Ag) on the uptake of SbIII or SbV by PV gametophytes. PV gametophytes were grown in 20% Hoagland solution containing 65 μM SbIII or SbV and increasing concentrations of analogs at 65-6500 μM for 2 h or 4 h under sterile condition. After exposing to 65 μM Sb for 2 h, PV accumulated 767 mg/kg Sb in SbIII treatment and 419 mg/kg in SbV treatment. SbIII uptake by PV gametophytes was not impacted by glycerol or AsIII nor aquaglyceroporin inhibitor Ag during 2 h exposure. While Si increased SbIII uptake and glucose decreased SbIII uptake by PV gametophytes, the impact disappeared during 4 h exposure. Under P-sufficient condition, P increased SbIII uptake and decreased SbV uptake during 2 h exposure, but the effect again disappeared after 4 h. After being P-starved for 2 weeks, P decreased SbIII with no effect on SbV uptake during 2 h exposure. Our results indicated that: 1) PV gametophytes could serve as an efficient model to study Sb uptake, and 2) unique SbIII uptake by PV may be related to its trait of As hyperaccumulation.
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Rujira
authors.initials: R
authors.lastname: Tisarum
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China.
authors.forename: Yanshan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Dong
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Lessl
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA. Electronic address: lqma@ufl.edu.
authors.forename: Lena Q
authors.initials: LQ
authors.lastname: Ma
mesh_terms: D000965:Antimony / Q000378:metabolism*; D051397:Aquaglyceroporins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D032495:Pteris / Q000187:drug effects / Q000378:metabolism*; D012834:Silver / Q000494:pharmacology*
chemical_list: D051397:Aquaglyceroporins; D010940:Plant Proteins; C061374:antimonite; D012834:Silver; D000965:Antimony; D001151:Arsenic
keywords: Antimony; Gametophyte; Glucose; Phosphate; Pteris vittata; Silicic acid