title: Neonatal streptozotocin-induced diabetes in mothers promotes metabolic programming of adipose tissue in male rat offspring.
abstract: OBJECTIVE
  Maternal hyperglycemia during pregnancy can lead to fetal changes, like macrosomia or obesity in adultlife. Experimentalmodels of diabetes have been studied to evaluate the consequences of offspring lipidmetabolism. This study aimed to investigate the metabolic changes in adipose tissue of offspring of streptozotocininduced diabetic mothers during neonatal period.
  
  
  METHODS
  Diabetes was induced in female rats by streptozotocin administration on 5th day of life. In adulthood, female rats were bred with control male rats. Male puppies were sacrificed on 12th week of life and epididymal (EP) and subcutaneous (SC) adipose fat pads were excised and weighted. Adipocytes were isolated and evaluated for basal and insulin-stimulated 2-deoxyglucose uptake, oxidation of glucose into CO2, and incorporationof glucose into lipids and lipolytic capacity.
  
  
  RESULTS
  Bodyweight, EP fat padweight and diameter of adipocytes fromoffspring of diabeticmothers were increased in comparison to offspring of control mothers. EP adipocytes from offspring of diabetic mothers presented increased basal and insulin stimulated glucose uptake in comparison to control ones. Similar pattern was observed for glucose oxidation into CO2 and incorporation into lipids. However, significant difference in lipolytic capacity in vitrowas not observed. Protein content of GLUT4, insulin receptor and acetyl-CoA carboxylase was significantly increased in EP fat pad of offspring of diabetic mothers in relation to control group.
  
  
  CONCLUSIONS
  Metabolic programming occurred in the adipose tissue of offspring of diabetic mothers, increasing its capacity to store lipids with no changes in lipolytic capacity.
authors.affiliation: Superior Institute of Biomedical Sciences, State University of Ceará, Brazil; Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil. Electronic address: ariclecio@hotmail.com.
authors.forename: Ariclécio C
authors.initials: AC
authors.lastname: Oliveira
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Sandra
authors.initials: S
authors.lastname: Andreotti
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Chimin
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Rogério A L
authors.initials: RA
authors.lastname: Sertié
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Talita da S M
authors.initials: Tda S
authors.lastname: Farias
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil; Department of Biophysics and Physiology, Federal University of Piauí, Brazil.
authors.forename: Francisco L
authors.initials: FL
authors.lastname: Torres-Leal
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: André R G
authors.initials: AR
authors.lastname: de Proença
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Campaña
authors.affiliation: Superior Institute of Biomedical Sciences, State University of Ceará, Brazil.
authors.forename: Liohanna S P
authors.initials: LS
authors.lastname: D'Avila
authors.affiliation: Superior Institute of Biomedical Sciences, State University of Ceará, Brazil.
authors.forename: Keciany A
authors.initials: KA
authors.lastname: Oliveira
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Fábio B
authors.initials: FB
authors.lastname: Lima
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D016640:Diabetes, Gestational / Q000139:chemically induced / Q000378:metabolism*; D005260:Female; D007328:Insulin / Q000097:blood; D008066:Lipolysis; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017208:Rats, Wistar; D013311:Streptozocin; D050151:Subcutaneous Fat / Q000378:metabolism* / Q000473:pathology
chemical_list: D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin
keywords: Adipose tissue; Insulin; Metabolic programming; Offspring of diabetic mothers; Streptozotocin