title: Galactose engineered solid lipid nanoparticles for targeted delivery of doxorubicin.
abstract: The present investigation reports the preparation, optimization, and characterization of surface engineered solid lipid nanoparticles (SLNs) encapsulated with doxorubicin (DOX). Salient features such as biocompatibility, controlled release, target competency, potential of penetration, improved physical stability, low cost and ease of scaling-up make SLNs viable alternative to liposomes for effective drug delivery. Galactosylation of SLNs instructs some gratifying characteristic, which leads to the evolution of promising delivery vehicles. The impendence of lectin receptors on different cell surfaces makes the galactosylated carriers admirable for targeted delivery of drugs to ameliorate their therapeutic index. Active participation of some lectin receptors in immune responses to antigen overlaid the application of galactosylated carriers in delivery of antigen and immunotherapy for treatment of maladies like cancer. These advantages revealed the promising potential of galactosylated carriers in each perspective of drug delivery. The developed DOX loaded galactosylated SLNs formulation was found to have particle size 239 ± 2.40 nm, PDI 0.307 ± 0.004, entrapment efficiency 72.3 ± 0.9%. Higher cellular uptake, cytotoxicity, and nuclear localization of galactosylated SLNs against A549 cells revealed higher efficiency of the formulation. In a nutshell, the galactosylation strategy with SLNs could be a promising approach in improving the delivery of DOX for cancer therapy.
authors.affiliation: Drug Delivery Research Group, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University, Chandigarh 160014, India; Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
authors.forename: Ashay
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India; Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA. Electronic address: prashant_pharmacy04@rediffmail.com.
authors.forename: Prashant
authors.initials: P
authors.lastname: Kesharwani
authors.affiliation: Drug Delivery Research Group, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University, Chandigarh 160014, India; Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
authors.forename: Neeraj K
authors.initials: NK
authors.lastname: Garg
authors.affiliation: Drug Delivery Research Group, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University, Chandigarh 160014, India; Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
authors.forename: Som Akshay
authors.initials: SA
authors.lastname: Jain
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Jain
authors.affiliation: Drug Delivery Research Group, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University, Chandigarh 160014, India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Nirbhavane
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
authors.forename: Raksha
authors.initials: R
authors.lastname: Ghanghoria
authors.affiliation: Department of Periodontics, College of Dental Medicine Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USA; Biosafety Support Unit, Regional Centre for Biotechnology-DBT, C.G.O. Complex, Lodhi Road, New Delhi 110003, India.
authors.forename: Rajeev Kumar
authors.initials: RK
authors.lastname: Tyagi
authors.affiliation: Drug Delivery Research Group, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University, Chandigarh 160014, India. Electronic address: drkatare@yahoo.com.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Katare
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004317:Doxorubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D005690:Galactose / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D000903:Antibiotics, Antineoplastic; D008055:Lipids; D004317:Doxorubicin; D005690:Galactose
keywords: Cytotoxicity; Doxorubicin; Galactose; Lectin; Solid lipid nanoparticles; Targeting