title: A novel Alaska pollack-derived peptide, which increases glucose uptake in skeletal muscle cells, lowers the blood glucose level in diabetic mice.
abstract: We found that the tryptic digest of Alaska pollack protein exhibits a glucose-lowering effect in KK-Ay mice, a type II diabetic model. We then searched for glucose-lowering peptides in the digest. Ala-Asn-Gly-Glu-Val-Ala-Gln-Trp-Arg (ANGEVAQWR) was identified from a peak of the HPLC fraction selected based on the glucose-lowering activity in an insulin resistance test using ddY mice. ANGEVAQWR (3 mg kg(-1)) decreased the blood glucose level after intraperitoneal administration. Among its fragment peptides, the C-terminal tripeptide, Gln-Trp-Arg (QWR, 1 mg kg(-1)), lowered the blood glucose level, suggesting that the C-terminal is critical for glucose-lowering activity. QWR also enhanced glucose uptake into C2C12, a mouse skeletal muscle cell line. QWR did not induce the phosphorylation of serine/threonine protein kinase B (Akt) and adenosine monophosphate-activated protein kinase (AMPK). We also demonstrated that QWR lowered the blood glucose level in NSY and KK-Ay, type II diabetic models.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Ayabe
authors.forename: Takafumi
authors.initials: T
authors.lastname: Mizushige
authors.forename: Wakana
authors.initials: W
authors.lastname: Ota
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kawabata
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Hayamizu
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsuji
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Kanamoto
authors.forename: Kousaku
authors.initials: K
authors.lastname: Ohinata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry*; D048388:Gadiformes; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D029941:Fish Proteins; D007328:Insulin; D010455:Peptides