title: Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases.
abstract: CagA of Helicobacter pylori undergoes tyrosine phosphorylation in a region containing differing numbers of repeat sequences (EPIYAs), which can result in a modulation of the inflammatory response. This study investigated whether the presence of CagA EPIYA variations in strains of H. pylori that are positive for this region contributes to differing degrees of disease severity in the gastric mucosa. In this study, 157 H. pylori-positive patients were included, and of those, 40.8% (64/157) were infected with cagA-positive strains, which were assayed for the presence of CagA EPIYA-ABC, EPIYA-ABCC, and EPIYA-ABCCC. Peptic ulcers were significantly more prevalent in patients infected with strains containing CagA EPIYA-ABCC/ABCCC than in those with CagA EPIYA ABC strains (P=0.044). This suggests that the number of repetitions of EPIYA-C influences the development of gastroduodenal lesions, highlighting the importance and usefulness of evaluating the cagA gene sequence when making therapeutic intervention decisions in patients infected with H. pylori.
authors.affiliation: Programa de Pós Graduação em Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário, Pelotas, Rio Grande do Sul, Brazil. Electronic address: jusvianna@hotmail.com.
authors.forename: Júlia Silveira
authors.initials: JS
authors.lastname: Vianna
authors.affiliation: Núcleo de Pesquisas em Microbiologia Médica, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Ivy Bastos
authors.initials: IB
authors.lastname: Ramis
authors.affiliation: Programa de Pós Graduação em Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário, Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Priscila Cristina Bartolomeu
authors.initials: PC
authors.lastname: Halicki
authors.affiliation: Hospital Universitário São Francisco de Paula (HUSFP), Universidade Católica de Pelotas, Rua Marechal Deodoro 1123, Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Otávio Leite
authors.initials: OL
authors.lastname: Gastal
authors.affiliation: Hospital Universitário São Francisco de Paula (HUSFP), Universidade Católica de Pelotas, Rua Marechal Deodoro 1123, Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Renato Azevedo
authors.initials: RA
authors.lastname: Silva
authors.affiliation: Hospital Universitário Dr. Miguel Riet Côrrea Jr, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: José Salomão
authors.initials: JS
authors.lastname: Junior
authors.affiliation: Hospital Universitário Dr. Miguel Riet Côrrea Jr, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Deise Machado
authors.initials: DM
authors.lastname: Dos Santos
authors.affiliation: Hospital Universitário Dr. Miguel Riet Côrrea Jr, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Chaves
authors.affiliation: Hospital Universitário Dr. Miguel Riet Côrrea Jr, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Carlos Renan
authors.initials: CR
authors.lastname: Juliano
authors.affiliation: Hospital Universitário São Francisco de Paula (HUSFP), Universidade Católica de Pelotas, Rua Marechal Deodoro 1123, Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Heitor Alberto
authors.initials: HA
authors.lastname: Jannke
authors.affiliation: Núcleo de Pesquisas em Microbiologia Médica, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Lande Vieira
authors.initials: LV
authors.lastname: da Silva
authors.affiliation: Núcleo de Pesquisas em Microbiologia Médica, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Von Groll
authors.affiliation: Núcleo de Pesquisas em Microbiologia Médica, Universidade Federal do Rio Grande (FURG), Rua General Osório, S/N, Rio Grande, Rio Grande do Sul, Brazil.
authors.forename: Pedro Eduardo Almeida
authors.initials: PE
authors.lastname: da Silva
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020816:Amino Acid Motifs; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001706:Biopsy; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D013272:Stomach Diseases / Q000382:microbiology* / Q000473:pathology*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori
keywords: Amino acid repeat sequence; Gastritis; Helicobacter pylori; Peptic ulcer; cagA protein