title: Biochanin A inhibits lipopolysaccharide-induced inflammation in human umbilical vein endothelial cells.
abstract: OBJECTIVE
  Biochanin A, an isoflavone isolated from red clover, cabbage or alfalfa, has been reported to have anti-inflammatory activity. However, the effects of biochanin A on vascular inflammation have not been investigated. In this study, we investigate the anti-inflammatory effects of biochanin A on lipopolysaccharide (LPS)-induced inflammatory response in human umbilical vein endothelial cells (HUVEC cells).
  
  
  METHODS
  The HUVEC cells were treated with biochanin A for 12h before exposure to LPS. The expression of ECAMs, including VCAM-1, ICAM-1, E-selectin, NF-κB and PPAR-γ was detected by Western blotting. The expression of cytokines TNF-α and IL-8 was detected by ELISA.
  
  
  RESULTS
  The results showed that biochanin A inhibited LPS-induced TNF-α and IL-8 production. Meanwhile, biochanin A also suppressed VCAM-1, ICAM-1, and E-selectin expression induced by LPS. We also found that biochanin A inhibited NF-κB activation induced by LPS. Furthermore, biochanin A could activate PPAR-γ and the anti-inflammatory effects of biochanin A can be reversed by GW9662, a specific antagonist for PPAR-γ.
  
  
  CONCLUSIONS
  In conclusion, the anti-inflammatory effect of biochanin A is associated with activating PPAR-γ, thereby attenuating NF-κB activation and LPS-induced inflammatory response. These findings suggest that biochanin A may be a therapeutic agent for inflammatory cardiovascular disease.
authors.affiliation: The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ming
authors.affiliation: The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Ding
authors.affiliation: The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhai
authors.affiliation: The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xiao
authors.affiliation: The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China.
authors.forename: Taikui
authors.initials: T
authors.lastname: Piao
authors.affiliation: The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, People's Republic of China. Electronic address: liuminglaos@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D019040:E-Selectin; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; D047495:PPAR gamma; C546936:SELE protein, human; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019833:Genistein; C004541:biochanin A
keywords: Biochanin A; Cytokine; Human umbilical vein endothelial cells; NF-κB; PPAR-γ