title: Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism.
abstract: The study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism. The subjects were PC12 cells. They were randomly divided into six groups: control, MPP(+) (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP(+) (250 μmol/L), Z-YVAD-CHO (10 nM) + MPP(+) group, and Z-DEVD-CHO (10 nM) + MPP(+) group. Cell viability was measured by MTT methods; the content of tyrosine hydroxylase was measured by immunocytochemistry method of avidinbiotin peroxidase complex; apoptosis ratio was measured by flow cytometry. The results showed that cell viability in the MPP(+) group was lower than in all other five groups. There was no difference in cell viability between isoflavone + MPP(+) and control group. Optical density of TH positive cells in isoflavone group was higher than in control, isoflavone + MPP(+), and MPP(+) only groups. The apoptosis ratio in the isoflavone + MPP(+) group and control group and the Z-YVAD-CHO + MPP(+) and Z-DEVD-CHO + MPP(+) group was similar, which was lower than in the MPP(+) group. The lowest apoptosis ratio was found in the isoflavone only group.
authors.affiliation: Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Xu
authors.affiliation: Department of General Surgery, The Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Song
authors.affiliation: Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.
authors.forename: Qing-Xia
authors.initials: QX
authors.lastname: Bu
authors.affiliation: Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.
authors.forename: De-Peng
authors.initials: DP
authors.lastname: Feng
authors.affiliation: Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Xu
authors.affiliation: Department of Neuroimmune Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.
authors.forename: Qian-Ru
authors.initials: QR
authors.lastname: Sun
authors.affiliation: Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020170:Caspase 1 / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007529:Isoflavones / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D020734:Parkinsonian Disorders / Q000188:drug therapy / Q000201:enzymology*; D051381:Rats
chemical_list: D007529:Isoflavones; D018696:Neuroprotective Agents; C505410:Casp3 protein, rat; D053148:Caspase 3; D020170:Caspase 1