title: Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping.
abstract: The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under continuous cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa.
authors.affiliation: College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China. heauzycxw@126.com.
authors.forename: Fengqing
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China. suoyanfei1990@163.com.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Institute of Industrial Crops, Henan Academy of Agricultural Science, Zhengzhou 450002, China. weihezj123@126.com.
authors.forename: He
authors.initials: H
authors.lastname: Wei
authors.affiliation: College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. xinyuzszj@163.com.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Li
authors.affiliation: School of medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China. nanyangxcx@126.com.
authors.forename: Caixia
authors.initials: C
authors.lastname: Xie
authors.affiliation: School of medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China. lnsdhz2012@126.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China. chenxinjian3978@126.com.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China. zyzhang@fafu.edu.cn.|College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. zyzhang@fafu.edu.cn.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016468:Forestry; D065928:Forests; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D032267:Rehmannia / Q000235:genetics* / Q000502:physiology; D013472:Sunlight; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: MYB transcription factor; Rehmannia glutinosa; continuous cropping; shading