title: Spectroscopic analysis and the excellent reusability of sphere-capped ferrocene in the oxidation of glucose oxidase.
abstract: Sphere-capped ferrocene nanospheres with Schiff base spacers have been prepared using a template, and used as carriers to immobilize glucose oxidase (GOx). GOx immobilized on spheres with one C-spacer (APS-Fc) exhibited high binding affinity to the substrate, which was attributed to appropriate position for the GOx conformation. When glucose oxidase was immobilized with spacers of different lengths, it was found that storage stability decreased with increasing the length of the spacer. It has been found that nanospheres, including capped ferrocene, exhibit good performance as the immobilized supporters of GOx. (APS-EtFc-GOx) retain more than 10% of the initial activity after forty-two successive cycles, which is a remarkable result.
authors.affiliation: a Department of Chemistry , Faculty of Science, Gazi University , Ankara , Turkiye.
authors.forename: Esin
authors.initials: E
authors.lastname: Antepli
authors.affiliation: a Department of Chemistry , Faculty of Science, Gazi University , Ankara , Turkiye.
authors.forename: Nurşen
authors.initials: N
authors.lastname: Sarı
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D000075163:Metallocenes; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D005656:Fungal Proteins; D000075163:Metallocenes; D005949:Glucose Oxidase; C004998:ferrocene
keywords: biocatalysts; ferrocene; glucose oxidase; spheres