title: [The influence of salvia miltiorhiza on the expression of TGF-β1 in periodontal tissue during orthodontic tooth movement in rats].
abstract: OBJECTIVE
  To investigate the effect of salvia miltiorhiza on the expression of TGF-β1 within periodontal tissues during orthodontic tooth movement in rat.
  
  
  METHODS
  One hundred and ninety-two male Sprague-Dawley rats were randomly divided into 4 groups (group A, B, C and D). Group A and B were used as orthodontic tooth movement model based on afterburner time. Group A, C and B, D were injected salvia miltiorhiza and NS under the buccal mucosa of left maxillary first molar every other day. Animals in each group were sacrificed at 6 time points (day 1,3,5,7,10 and 14) after appliance placement for collection of specimens. The distance of the tooth movement was measured by stereomicroscope, periodontal tissue was investigated by H-E staining and the protein expression of TGF-β1 was examined by immunohistochemical techniques. SPSS17.0 software package was used for statistical analysis.
  
  
  RESULTS
  Except for the first day, the protein expression of TGF-β1 in group A was highest and there was significant up-regulation on day 3, with a peak on day 5. Compared with group B, the average optical density of TGF-β1 in group A was 0.5181±0.0037 on day 3, 0.5857±0.00235 on day 5 and 0.4363±0.0021 on day 7, both with significant differences (P<0.01). Compared with group C and group D, significant differences were formed at each time point in group A (P<0.05).
  
  
  CONCLUSIONS
  Salvia injection may improve periodontal tissue microcirculation,thus promote the expression of TGF-β1 protein,which may be one of the mechanisms to accelerate orthodontic tooth movement. Supported by Research Fund of Science and Technology of Honghuagang District, Zunyi City[(2010)11].
authors.affiliation: Department of Orthodontic, Zunyi Medical College Affiliated Stomatology Hospital. Zunyi 563003, Guizhou Province, China.E-mail:zhangjiangtao75@sina.com.
authors.forename: Jiang-tao
authors.initials: JT
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Mei
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Yue-rong
authors.initials: YR
authors.lastname: Zhang
authors.forename: Ce
authors.initials: C
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Jia-jia
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D008297:Male; D008963:Molar; D010519:Periodontium; D051381:Rats; D017207:Rats, Sprague-Dawley; D027544:Salvia; D027561:Salvia miltiorrhiza; D014087:Tooth Movement Techniques; D053773:Transforming Growth Factor beta1
chemical_list: D053773:Transforming Growth Factor beta1