title: Antigenotoxic effects of resveratrol: assessment of in vitro and in vivo response.
abstract: Experiments were performed to evaluate the in vitro and in vivo dose response for antigenotoxic effects of resveratrol (RES). For the in vitro study, HL-60 cells were co-treated with the test genotoxin and three concentrations of RES. Thereafter, genotoxic effects were assessed in the cytokinesis-block micronucleus test. Results of the in vitro experiments using genotoxins nitroquinoline-1-oxide (NQO) and mitomycin C (MMC) showed maximum inhibition of genotoxicity with the lowest test concentration of RES. The mouse bone marrow micronucleus assay was used for evaluating the in vivo antigenotoxic effects of RES against genotoxins diepoxybutane (DEB), MMC, methyl methanesulfonate and procarbazine (PCB). The experimental animals received RES pre-treatment by gavage 30min, 24 and 48h before injecting the genotoxin intraperitoneally. The in vivo studies demonstrated efficacy of the lowest test dose of RES for exerting maximum protection against chromosomal damage induced by all four genotoxins. The antigenotoxic effect observed with 6.25mg/kg RES was significantly higher than that of 100mg/kg RES against PCB and DEB. In conclusion, the findings from the present study indicate that lower test concentrations/doses of RES are more effective in exerting antigenotoxic effects.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Abraham
authors.forename: Nidhi
authors.initials: N
authors.lastname: Khandelwal
authors.affiliation: Institut für Pharmakologie und Toxikologie, Universität Würzburg 97078, Würzburg, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Hintzsche
authors.affiliation: Institut für Pharmakologie und Toxikologie, Universität Würzburg 97078, Würzburg, Germany Stopper@toxi.uni-wuerzburg.de.
authors.forename: Helga
authors.initials: H
authors.lastname: Stopper
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D004249:DNA Damage; D004852:Epoxy Compounds / Q000633:toxicity; D018922:HL-60 Cells; D006801:Humans; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D011344:Procarbazine / Q000633:toxicity; D015363:Quinolones / Q000633:toxicity; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C120195:4-nitroquinolone-1-oxide; D016587:Antimutagenic Agents; D004852:Epoxy Compounds; D015363:Quinolones; D013267:Stilbenes; D011344:Procarbazine; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide; C007366:diepoxybutane; D008741:Methyl Methanesulfonate; D000077185:Resveratrol