title: Analyte-Size-Dependent Ionization and Quantification of Monosaccharides in Human Plasma Using Cation-Exchanged Smectite Layers.
abstract: Smectite, a synthetic inorganic polymer with a saponite structure, was subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Typical organic matrix molecules 2,4,6-trihydroxyacetophenone (THAP) and 2,5-dihydroxybenzoic acid (DHBA) were intercalated into the layer spacing of cation-exchanged smectite, and the complex was used as a new matrix for laser desorption/ionization mass spectrometry. Because of layer spacing limitations, only a small analyte that could enter the layer and bind to THAP or DHBA could be ionized. This was confirmed by examining different analyte/matrix preparation methods and by measuring saccharides with different molecular sizes. Because of the homogeneous distribution of THAP molecules in the smectite layer spacing, high reproducibility of the analyte peak intensity was achieved. By using isotope-labeled (13)C6-d-glucose as the internal standard, quantitative analysis of monosaccharides in pretreated human plasma sample was performed, and the value of 8.6 ± 0.3 μg/mg was estimated.
authors.affiliation: †Department of Food Inspection, Zhejiang Institute for Food and Drug Control, No. 86, Lane 1, Jichang Road, Jianggan District, Hangzhou, Zhejiang, China.|‡Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: ‡Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Kawakita
authors.affiliation: ‡Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Xu
authors.affiliation: ‡Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Akiyama
authors.affiliation: ‡Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujino
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007477:Ions / Q000737:chemistry; D008970:Molecular Weight; D009005:Monosaccharides / Q000097:blood*; D015203:Reproducibility of Results; D017640:Silicates / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007477:Ions; D009005:Monosaccharides; D017640:Silicates; C033214:Smectite