title: C6 Peptide-Based Multiplex Phosphorescence Analysis (PHOSPHAN) for Serologic Confirmation of Lyme Borreliosis.
abstract: BACKGROUND
  A single-tier immunoassay using the C6 peptide of VlsE (C6) from Borrelia burgdorferi sensu stricto (Bb) has been proposed as a potential alternative to conventional two-tier testing for the serologic diagnosis of Lyme disease in the United States and Europe.
  
  
  OBJECTIVE
  To evaluate the performance of C6 peptide based multiplex Phosphorescence Analysis (PHOSPHAN) for the serologic confirmation of Lyme borreliosis (LB) in Russian patients.
  
  
  METHODS
  Serum samples (n = 351) were collected from 146 patients with erythema migrans (EM); samples from 131 of these patients were taken several times prior to treatment and at different stages of recovery. The control group consisted of 197 healthy blood donors and 31 patients with other diseases, all from the same highly endemic region of Russia. All samples were analyzed by PHOSPHAN for IgM and IgG to Bb C6, recombinant OspC and VlsE proteins, and C6 peptides from B. garinii and B. afzelii.
  
  
  RESULTS
  IgM and IgG to Bb C6 were identified in 43 and 95 out of 131 patients (32.8 and 72.5%, respectively); seroconversion of IgM antibodies was observed in about half of the patients (51.2%), and of IgG antibodies, in almost all of them (88.4%). Additional detection of OspC-IgM and VlsE-IgM or IgG to C6 from B. garinii or B. afzelii did not contribute significantly to the overall sensitivity of the multiplex immunoassay.
  
  
  CONCLUSIONS
  The multiplex phosphorescence immunoassay is a promising method for simultaneously revealing the spectrum of antibodies to several Borrelia antigens. Detection of IgM and IgG to Bb C6 in the sera of EM patients provides effective serologic confirmation of LB and, with high probability, indicates an active infection process.
authors.affiliation: Laboratory of Molecular Diagnostics, Department of Biological Microassay, State Research Institute of Biological Engineering, Moscow, Russian Federation.
authors.forename: Vera G
authors.initials: VG
authors.lastname: Pomelova
authors.affiliation: Department of Infections with Natural Focality, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation.
authors.forename: Edward I
authors.initials: EI
authors.lastname: Korenberg
authors.affiliation: Laboratory of Clinical Immunology, Clinical Infectious Diseases Hospital, Perm, Russian Federation.
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Kuznetsova
authors.affiliation: Laboratory of Molecular Diagnostics, Department of Biological Microassay, State Research Institute of Biological Engineering, Moscow, Russian Federation.
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Bychenkova
authors.affiliation: Laboratory of Molecular Diagnostics, Department of Biological Microassay, State Research Institute of Biological Engineering, Moscow, Russian Federation.
authors.forename: Natalya I
authors.initials: NI
authors.lastname: Bekman
authors.affiliation: Immunoscreen Closed Joint Stock Company, Moscow, Russian Federation.
authors.forename: Nikolay S
authors.initials: NS
authors.lastname: Osin
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology; D016022:Case-Control Studies; D015787:Erythema Chronicum Migrans; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology; D008163:Luminescent Measurements / Q000379:methods; D008193:Lyme Disease / Q000276:immunology*; D008875:Middle Aged; D010455:Peptides / Q000276:immunology*; D012426:Russia; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008074:Lipoproteins; C079616:OspC protein; D010455:Peptides; C105546:VlsE protein, Borrelia burgdorferi