title: Surface-enhanced laser desorption ionization time-of-flight mass spectrometry used to screen serum diagnostic markers of colon cancer recurrencein situfollowing surgery.
abstract: The aim of the present study was to identify specific serum biomarkers in patients with colon cancer recurrencein situfollowing surgery. The study was conducted at the Renmin Hospital of Wuhan University (Wuhan, China) between January 2012 and January 2014. Surface-enhanced laser desorption ionization time-of-flight mass spectrometry was used to compare and analyze the serum protein profiles of patients with (n=50) and patients without (n=50) recurrencein situ. Biomarker Wizard software was used to analyze and obtain the protein spectrum. In total, nine protein peaks demonstrated statistically significant differences between the recurrence and non-recurrence group (P<0.05), which included two protein peaks (7,731.3 Da and 8,266.5 Da). The two protein peaks were highly expressed in patients with colon cancer recurrencein situfollowing surgery, but lowly expressed in patients without recurrence. Therefore, the two protein peaks may represent potential biomarkers for the prediction of colon cancer recurrencein situfollowing surgical treatment.
authors.affiliation: Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Zhong-Yin
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Ji
authors.affiliation: Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Luo
keywords: biological marker; colon cancer; recurrence; surface-enhanced laser desorption/ionization time-of-flight mass spectrometry