title: [Markers for Immunofluorescence Analysis Based on Europium Complex with Fluorinated β-Diketones Carbazole Series].
abstract: In order to develop new markers for immunofluorescence time-resolved luminescence analysis tetraketodiester carbazole series was obtained. Compound contains methoxycarbonyl reactive groups, separated from the 1,3-dicarbonyl chelating fragment by difluoromethylene spacers (CF2)4. Complexes of this compound with the ions Eu3+ are stable in aqueous solution and possess a long-living intensive luminescence with the main peaks: emission--in the area of 615 nm and excitation--in the 380-390 nm, that distinguishes them from most used analogues. Conjugate of streptavidin to compound was obtained and its fluorescent spectral characteristics allow to use it as a universal reagent for various schemes of biological microanalysis.
authors.forename: T S
authors.initials: TS
authors.lastname: Kostryukova
authors.forename: N P
authors.initials: NP
authors.lastname: Ivanovskaya
authors.forename: G V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: N S
authors.initials: NS
authors.lastname: Osin
authors.forename: N V
authors.initials: NV
authors.lastname: Vasilev
mesh_terms: D002227:Carbazoles / Q000737:chemistry*; D002614:Chelating Agents / Q000138:chemical synthesis* / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D019084:Fluorescent Antibody Technique, Indirect; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D002227:Carbazoles; D002614:Chelating Agents; D006845:Hydrocarbons, Fluorinated; D005063:Europium