title: Rapid Screening of Multiclass Syrup Adulterants in Honey by Ultrahigh-Performance Liquid Chromatography/Quadrupole Time of Flight Mass Spectrometry.
abstract: Honey adulteration with sugar syrups is a widespread problem. Several types of syrups have been used in honey adulteration, and there is no available method that can simultaneously detect all of these adulterants. In this study, we generated a small-scale database containing the specific chromatographic and mass spectrometry information on sugar syrup markers and developed a simple, rapid, and effective ultrahigh-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) method for the detection of adulterated honey. Corn syrup, high-fructose corn syrup, inverted syrup, and rice syrup were used as honey adulterants; polysaccharides, difructose anhydrides, and 2-acetylfuran-3-glucopyranoside were used as detection markers. The presence of 10% sugar syrup in honey could be easily detected in <30 min using the developed method. The results revealed that UHPLC/Q-TOF-MS was simple and rapid.
authors.affiliation: †Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.|‡Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Du
authors.affiliation: §Risk Assessment Laboratory for Bee Products Quality and Safety, Ministry of Agriculture, Beijing 100093, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wu
authors.affiliation: ‡Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.|§Risk Assessment Laboratory for Bee Products Quality and Safety, Ministry of Agriculture, Beijing 100093, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Xue
authors.affiliation: ‡Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.|§Risk Assessment Laboratory for Bee Products Quality and Safety, Ministry of Agriculture, Beijing 100093, China.
authors.forename: Lanzhen
authors.initials: L
authors.lastname: Chen
authors.affiliation: §Risk Assessment Laboratory for Bee Products Quality and Safety, Ministry of Agriculture, Beijing 100093, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: ⊗Bee Product Quality Supervisionand Testing Center, Ministry of Agriculture, Beijing 102202, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: †Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004187:Disaccharides / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D004187:Disaccharides; D011134:Polysaccharides
keywords: UHPLC/Q-TOF-MS; adulterated honey; multiclass sugar syrups