title: [Effects of lipopolysaccharide and phytohaemagglutinin on the level of microRNAs in mouse peripheral blood mononuclear cells].
abstract: OBJECTIVE
  To investigate the effects of lipopolysaccharide (LPS) and phytohaemagglutinin (PHA) on the level of microRNAs in mouse peripheral blood mononuclear cells (PBMCs).
  
  
  METHODS
  The mouse PBMCs were cultured in vitro and stimulated with 100 ng/mL LPS for 1 hour or 2 hours and 2.5 μg/mL phytohaemagglutinin (PHA) for 2 hours or 4 hours, respectively. The levels of miR-9, miR-122-3p, miR-181d and miR-342-3p in both PBMCs and culture supernatants were determined by real-time quantitative PCR (qRT-PCR).
  
  
  RESULTS
  The levels of miR-9, miR-122-3p, miR-181d and miR-342-3p in PBMCs and culture supernatants had no significant difference between blank control and LPS stimulation group, nor did between LPS stimulation 2 hours group and 1 hour group. The levels of miR-9, miR-122-3p and miR-342-3p in PBMCs and culture supernatant had no significant difference between blank control and PHA stimulation group, nor did between PHA stimulation 4 hours group and 2 hours group. However, compared with blank control, the expression of miR-181d significantly increased after PHA stimulation, while the time of stimulation did not affect the miR-181d level significantly.
  
  
  CONCLUSIONS
  The stimulation of PHA could promote the expression and secretion of miR-181d in mouse PBMCs.
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Pathogenic Biology, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Wenhong
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Qiugui
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yuyun
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Jiajing
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, School of Medicine, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Jiao
mesh_terms: D000818:Animals; D017077:Culture Media, Conditioned / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000097:blood*; D010835:Phytohemagglutinins / Q000494:pharmacology*
chemical_list: D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D035683:MicroRNAs; D010835:Phytohemagglutinins