title: Tartary buckwheat on nitric oxide-induced inflammation in RAW264.7 macrophage cells.
abstract: We investigated the effects of tartary buckwheat (TB, Fagopyrum tataricum) on the production of pro-inflammatory mediators in lipopolysaccharide (LPS)- and interferon (IFN)-γ-stimulated RAW264.7 cells. We evaluated the anti-inflammatory effects of TB against the LPS- and IFN-γ-stimulated inflammatory response in RAW264.7 macrophage cells. We fractionated TB to obtain 4 fractions including the n-hexane, methylene chloride, ethyl acetate (EtOAc), and n-butanol fractions. In addition, rutin was isolated and identified from the EtOAc fraction. The 4 fractions and rutin effectively inhibited the production of reactive oxygen species, nitric oxide (NO), and interleukin-6. In addition, the mRNA expression levels of pro-inflammatory factors including nuclear factor kappa B, cyclooxygenase-2, and inducible NO synthase were down-regulated in LPS- and IFN-γ-stimulated RAW264.7 cells following treatment with the 4 fractions and rutin. The present study suggests that TB could induce anti-inflammation by regulating the expression of inflammatory mediators.
authors.affiliation: Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea. ejcho@pusan.ac.kr.
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Choi
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Choi
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Cho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000276:immunology; D019612:Fagopyrum / Q000737:chemistry*; D007249:Inflammation / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D009569:Nitric Oxide / Q000009:adverse effects; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D010936:Plant Extracts; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2