title: [Molecular identification of hairy antler by analysis of high resolution melting].
abstract: High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further optimized. The results showed that the Tm values of Cervus nippon were (81.96 ± 0.07), (84.51 ± 0.03) °C and Cervus elaphus was(82.58 ± 0.13), (85.95 ± 0.05) °C with 10-100 mg · L(-1) DNA template, 0.2 µLmol · L(-1) primer, 2.0 mmol · L(-1) Mg2+. This method can authenticate of hairy antler and is simple, fast, high-throughput, visualization.
authors.forename: Kang
authors.initials: K
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003670:Deer / Q000145:classification / Q000235:genetics*; D005838:Genotype; D008516:Medicine, Chinese Traditional / Q000592:standards; D016133:Polymerase Chain Reaction; D044366:Transition Temperature
chemical_list: D017931:DNA Primers; D004247:DNA