title: Approaching the Golden Fleece a Molecule at a Time: Biophysical Insights into Argonaute-Instructed Nucleic Acid Interactions.
abstract: Argonaute proteins act at the core of nucleic acid-guided interference pathways that regulate gene expression and defend organisms against foreign genetic elements in all domains of life. Here, we review recent biophysical studies on how Argonaute proteins instruct oligonucleotides in the process of target finding, binding, cleavage, and release, as measured at high spatiotemporal resolution by single-molecule approaches. In the context of previous structural, biochemical, and computational studies, a model emerges for how Argonaute proteins manipulate the thermodynamic rules for nucleic acid hybridization to convey efficiency and specificity to RNA- and DNA-guided regulatory processes.
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
authors.forename: Veronika A
authors.initials: VA
authors.lastname: Herzog
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohr-Gasse 3, 1030 Vienna, Austria. Electronic address: stefan.ameres@imba.oeaw.ac.at.
authors.forename: Stefan L
authors.initials: SL
authors.lastname: Ameres
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics*; D012313:RNA / Q000235:genetics; D013816:Thermodynamics
chemical_list: D060565:Argonaute Proteins; D009841:Oligonucleotides; D012313:RNA; D004247:DNA