title: 6-Deoxy-6-aminoethyleneamino cellulose: synthesis and study of hemocompatibility.
abstract: Hemocompatibility of aqueous solutions of antimicrobial 6-deoxy-6-aminoethyleneamino (AEA) cellulose with different degrees of substitution (DS, 0.54-0.92) was investigated in vitro. The AEA cellulose derivatives were synthesized by tosylation of cellulose and subsequent nucleophilic substitution with 1,2-diaminoethane. The structure was confirmed by elemental analysis as well as by FTIR and NMR spectroscopies. Markers for coagulation (thrombin generation, aPTT, PT, blood clotting, thrombocyte activation) and membrane integrity (hemolysis) were measured in human whole blood, human platelet-rich plasma, human pooled plasma, and erythrocytes suspension. AEA cellulose with a low DS of 0.54 showed the highest hemocompatibility in vitro, suggesting the possibility of biomedical applications.
authors.affiliation: a Department of Dermatology , University Medical Center Jena , Erfurter Straße 35, 07743 Jena , Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Zieger
authors.forename: Michael
authors.initials: M
authors.lastname: Wurlitzer
authors.forename: Cornelia
authors.initials: C
authors.lastname: Wiegand
authors.forename: Kirsten
authors.initials: K
authors.lastname: Reddersen
authors.forename: Susanne
authors.initials: S
authors.lastname: Finger
authors.forename: Peter
authors.initials: P
authors.lastname: Elsner
authors.forename: Peggy
authors.initials: P
authors.lastname: Laudeley
authors.forename: Tim
authors.initials: T
authors.lastname: Liebert
authors.forename: Thomas
authors.initials: T
authors.lastname: Heinze
authors.forename: Uta-Christina
authors.initials: UC
authors.lastname: Hipler
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry*; D001777:Blood Coagulation; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002482:Cellulose / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000502:physiology; D005029:Ethylenediamines / Q000737:chemistry; D005434:Flow Cytometry; D006461:Hemolysis; D006801:Humans; D008422:Materials Testing; D015394:Molecular Structure; D010949:Plasma / Q000502:physiology; D015539:Platelet Activation; D013917:Thrombin / Q000378:metabolism; D013997:Time Factors; D014105:Tosyl Compounds / Q000737:chemistry
chemical_list: C000607228:6-deoxy-6-aminoethyleneamino cellulose; D001672:Biocompatible Materials; D005029:Ethylenediamines; D014105:Tosyl Compounds; C031234:ethylenediamine; D002482:Cellulose; D013917:Thrombin; D004121:Dimethyl Sulfoxide
keywords: 6-deoxy-6-aminoethyleneamino cellulose; degree of substitution; hemocompatibility