title: Comparison of the anti-diabetic effects of resveratrol, gliclazide and losartan in streptozotocin-induced experimental diabetes.
abstract: OBJECTIVE
  The aim of this study was to compare the effect of the resveratrol with gliclazide and losartan in streptozotocin induced diabetic rats.
  
  
  METHODS
  Adult male Wistar albino rats were divided into five groups of seven rats each. Diabetes was induced with a single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Rats with blood glucose levels above 250 mg/dl after 48 h of streptozotocin injection were included in the diabetic group. Gliclazide and resveratrol were administered for 3 weeks at 5 mg/kg per day and losartan was administered for 3 weeks at 30 mg/kg per day in an oral aqueous suspension. At the end of the third week all rats were euthanized and fasting blood glucose, HbA1c and the metabolic activity of the hepatic enzymes hexokinase and glucose-6 phosphate dehydrogenase were measured in tail blood and liver specimens. All parameters were quantified using an ELISA plate reader.
  
  
  RESULTS
  Resveratrol and gliclazide significantly reduced both blood glucose levels and HbA1c levels in diabetic rats (p < 0.001). However, losartan did not exhibit the same effects (p < 0.05). The enzymatic activity of the liver enzymes hexokinase, glucose-6 phosphate dehydrogenase, fructose 1,6-biphosphatase, pyruvate kinase and glucose-6 phosphatase were enhanced by resveratrol and gliclazide, while losartan treatment was not associated with significant changes in liver carbohydrate metabolism.
  
  
  CONCLUSIONS
  Resveratrol was not effective in improving liver carbohydrate metabolism relative to gliclazide, a drug widely used to treat diabetes. Dose-response profile of resveratrol remains indeterminate and additional studies may be necessary to determine effective dosing in diabetes.
authors.affiliation: a Zirve University, School of Medicine, Department of Physiology , Gaziantep , Turkey .
authors.forename: Ümit Can
authors.initials: ÜC
authors.lastname: Yazgan
authors.affiliation: b Medical Park Hospital, Department of Internal Medicine , Antalya , Turkey .
authors.forename: Ezel
authors.initials: E
authors.lastname: Taşdemir
authors.affiliation: c Dicle University, School of Medicine, Department of Physiology , Diyarbakır , Turkey , and.
authors.forename: Hakkı Murat
authors.initials: HM
authors.lastname: Bilgin
authors.affiliation: c Dicle University, School of Medicine, Department of Physiology , Diyarbakır , Turkey , and.
authors.forename: Basra
authors.initials: B
authors.lastname: Deniz Obay
authors.affiliation: c Dicle University, School of Medicine, Department of Physiology , Diyarbakır , Turkey , and.
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Şermet
authors.affiliation: d Dicle University, School of Medicine, Department of Immunology , Diyarbakır , Turkey.
authors.forename: Bilal
authors.initials: B
authors.lastname: Elbey
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D005907:Gliclazide / Q000494:pharmacology / Q000627:therapeutic use; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D019808:Losartan / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D001786:Blood Glucose; D005907:Gliclazide; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D019808:Losartan; D000077185:Resveratrol; D013267:Stilbenes
keywords: Diabetes; gliclazide; resveratrol; streptozotocin