title: Real-time monitoring of geosmin and 2-methylisoborneol, representative odor compounds in water pollution using bioelectronic nose with human-like performance.
abstract: A bioelectronic nose for the real-time assessment of water quality was constructed with human olfactory receptor (hOR) and single-walled carbon nanotube field-effect transistor (swCNT-FET). Geosmin (GSM) and 2-methylisoborneol (MIB), mainly produced by bacteria, are representative odor compounds and also indicators of contamination in the water supply system. For the screening of hORs which respond to these compounds, we performed CRE-luciferase assays of the two odorants in heterologous cell system. Human OR51S1 for GSM and OR3A4 for MIB were selected, and nanovesicles expressing the hORs on surface were produced from HEK-293 cell. Carbon nanotube field-effect transistor was functionalized with the nanovesicles. The bioelectronic nose was able to selectively detect GSM and MIB at concentrations as low as a 10 ng L(-1). Furthermore, detection of these compounds from the real samples such as tap water, bottled water and river water was available without any pretreatment processes.
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Manki
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Dong-guk
authors.initials: DG
authors.lastname: Cho
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Lim
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea; Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
authors.affiliation: N-Bio Institute, Seoul National University, Seoul 151-818, Republic of Korea. Electronic address: hwijinko@snu.ac.kr.
authors.forename: Hwi Jin
authors.initials: HJ
authors.lastname: Ko
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea; Advanced Institutes of Convergence Technology, Suwon 433-270, Republic of Korea. Electronic address: thpark@snu.ac.kr.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001892:Camphanes / Q000032:analysis* / Q000378:metabolism; D062609:Electronic Nose; D004867:Equipment Design; D057809:HEK293 Cells; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D009284:Naphthols / Q000032:analysis* / Q000378:metabolism; D009812:Odorants / Q000032:analysis*; D018035:Receptors, Odorant / Q000378:metabolism; D014876:Water Pollution / Q000032:analysis*; D060753:Water Quality
chemical_list: D001892:Camphanes; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D009284:Naphthols; D018035:Receptors, Odorant; C005536:2-methylisoborneol; C001278:geosmin
keywords: Bioelectronic nose; Carbon nanotube; Field-effect transistor; Human olfactory receptor; Water quality monitoring