title: A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNAIle(GAU).
abstract: Plastid gene expression is crucial for organelle function, but the factors that control it are still largely unclear. Members of the so-called mitochondrial transcription termination factor (mTERF) family are found in metazoans and plants and regulate organellar gene expression at different levels. Arabidopsis (Arabidopsis thaliana) mTERF6 is localized in chloroplasts and mitochondria, and its knockout perturbs plastid development and results in seedling lethality. In the leaky mterf6-1 mutant, a defect in photosynthesis is associated with reduced levels of photosystem subunits, although corresponding messenger RNA levels are unaffected, whereas translational capacity and maturation of chloroplast ribosomal RNAs (rRNAs) are perturbed in mterf6-1 mutants. Bacterial one-hybrid screening, electrophoretic mobility shift assays, and coimmunoprecipitation experiments reveal a specific interaction between mTERF6 and an RNA sequence in the chloroplast isoleucine transfer RNA gene (trnI.2) located in the rRNA operon. In vitro, recombinant mTERF6 bound to its plastid DNA target site can terminate transcription. At present, it is unclear whether disturbed rRNA maturation is a primary or secondary defect. However, it is clear that mTERF6 is required for the maturation of trnI.2. This points to an additional function of mTERFs.
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Isidora
authors.identifier: http://orcid.org/0000-0003-3001-5767
authors.initials: I
authors.lastname: Romani
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Nikolay
authors.identifier: http://orcid.org/0000-0003-2740-5991
authors.initials: N
authors.lastname: Manavski
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Arianna
authors.initials: A
authors.lastname: Morosetti
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Luca
authors.identifier: http://orcid.org/0000-0003-2315-7695
authors.initials: L
authors.lastname: Tadini
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Swetlana
authors.initials: S
authors.lastname: Maier
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Kristina
authors.identifier: http://orcid.org/0000-0001-6747-783X
authors.initials: K
authors.lastname: Kühn
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Hannes
authors.initials: H
authors.lastname: Ruwe
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Christian
authors.initials: C
authors.lastname: Schmitz-Linneweber
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wanner
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.).
authors.forename: Dario
authors.identifier: http://orcid.org/0000-0003-1897-8421
authors.initials: D
authors.lastname: Leister
authors.affiliation: Plant Molecular Biology (Botany), Department Biology I (I.R., N.M., A.M., L.T., D.L., T.K.), and Ultrastrukturforschung, Department Biology I (G.W.), Ludwig-Maximilians-Universität München, 81252 Planegg-Martinsried, Germany;Mathematisch-Naturwissenschaftliche Fakultät I/Biologie, Molekulare Zellbiologie der Pflanzen, Humboldt-Universität zu Berlin, 10099 Berlin, Germany (S.M., K.K.); andInstitute of Biology, Molecular Genetics, Humboldt-University of Berlin, 10115 Berlin, Germany (H.R., C.S.-L.) tatjana.kleine@lmu.de.
authors.forename: Tatjana
authors.identifier: http://orcid.org/0000-0001-6455-3470
authors.initials: T
authors.lastname: Kleine
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D049148:Aminoacylation; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D056426:Genetic Loci; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D010788:Photosynthesis; D011485:Protein Binding; D021381:Protein Transport; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism; D012639:Seeds / Q000648:ultrastructure; D061806:Transcription Termination, Genetic
chemical_list: D020121:5' Untranslated Regions; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D004269:DNA, Bacterial; D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012355:RNA, Transfer, Ile; C036483:T-DNA; C000608152:mTERF6 protein, Arabidopsis