title: Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress.
abstract: One of the critical alarming constraints for agriculture is water scarcity. In the current scenario, global warming due to climate change and unpredictable rainfall, drought is going to be a master player and possess a big threat to stagnating gene pool of staple food crops. So it is necessary to understand the mechanisms that enable the plants to cope with drought stress. In this study, effort was made to prospect the role of EcDehydrin7 protein from normalized cDNA library of drought tolerance finger millet in transgenic tobacco. Biochemical and molecular analyses of T0 transgenic plants were done for stress tolerance. Leaf disc assay, seed germination test, dehydration assay, and chlorophyll estimation showed EcDehydrin7 protein directly link to drought tolerance. Northern and qRT PCR analyses shows relatively high expression of EcDehydrin7 protein compare to wild type. T0 transgenic lines EcDehydrin7(11) and EcDehydrin7(15) shows superior expression among all lines under study. In summary, all results suggest that EcDehydrin7 protein has a remarkable role in drought tolerance and may be used for sustainable crop breeding program in other food crops.
authors.affiliation: National Research Centre on Plant Biotechnology, LBS Building, Pusa Campus, New Delhi, 110012, India.
authors.forename: Rajiv Kumar
authors.initials: RK
authors.lastname: Singh
authors.forename: Vivek Kumar
authors.initials: VK
authors.lastname: Singh
authors.forename: Sanagala
authors.initials: S
authors.lastname: Raghavendrarao
authors.forename: Mullapudi Lakshmi Venkata
authors.initials: ML
authors.lastname: Phanindra
authors.forename: K
authors.initials: K
authors.lastname: Venkat Raman
authors.forename: Amolkumar U
authors.initials: AU
authors.lastname: Solanke
authors.forename: Polumetla Ananda
authors.initials: PA
authors.lastname: Kumar
authors.forename: Tilak Raj
authors.initials: TR
authors.lastname: Sharma
mesh_terms: D000222:Adaptation, Physiological; D002734:Chlorophyll / Q000378:metabolism; D003001:Cloning, Molecular; D055864:Droughts; D031748:Eleusine / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005822:Genetic Vectors / Q000378:metabolism; D018525:Germination; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000254:growth & development; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics* / Q000502:physiology*; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant; D002734:Chlorophyll