title: Myogenic induction of adult and pluripotent stem cells using recombinant proteins.
abstract: Met Activating Genetically Improved Chimeric Factor 1 (Magic-F1) is a human recombinant protein, derived from dimerization of the receptor-binding domain of hepatocyte growth factor. Previous experiments demonstrate that in transgenic mice, the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, improving running performance and accelerating muscle regeneration after injury. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells. In murine mesoangioblasts (adult vessel-associated stem cells), the presence of Magic-F1 did not alter their osteogenic, adipogenic or smooth muscle differentiation ability. However, when analyzing their myogenic potential, mesoangioblasts expressing Magic-F1 differentiated spontaneously into myotubes. Finally, Magic-F1 inducible cassette was inserted into a murine embryonic stem cell line by homologous recombination. When embryonic stem cells were subjected to myogenic differentiation, the presence of Magic-F1 resulted in the upregulation of Pax3 and Pax7 that enhanced the myogenic commitment of transgenic pluripotent stem cells. Taken together our results candidate Magic-F1 as a potent myogenic stimulator, able to enhance muscular differentiation from both adult and pluripotent stem cells.
authors.affiliation: Translational Cardiomyology Laboratory, Embryo and Stem Cell Biology Unit, Dept of Development and Regeneration, KU Leuven, Belgium.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Perini
authors.affiliation: Translational Cardiomyology Laboratory, Embryo and Stem Cell Biology Unit, Dept of Development and Regeneration, KU Leuven, Belgium; Vesalius Research Center, Belgium and Department of Oncology, VIB, KU Leuven, Belgium.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Elia
authors.affiliation: Mediterranean Institute for Transplantation and Advanced Specialized Therapies (IRCCS-ISMETT), Regenerative Medicine and Biomedical Technologies Unit, Palermo, Italy; Embryo and Stem Cell Biology Unit, Dept of Development and Regeneration, KU Leuven, Belgium.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lo Nigro
authors.affiliation: Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Switzerland.
authors.forename: Flavio
authors.initials: F
authors.lastname: Ronzoni
authors.affiliation: Translational Cardiomyology Laboratory, Embryo and Stem Cell Biology Unit, Dept of Development and Regeneration, KU Leuven, Belgium.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Berardi
authors.affiliation: Translational Cardiomyology Laboratory, Embryo and Stem Cell Biology Unit, Dept of Development and Regeneration, KU Leuven, Belgium.
authors.forename: Hanne
authors.initials: H
authors.lastname: Grosemans
authors.affiliation: Laboratory of Molecular and Cellular Physiology, Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: So-ichiro
authors.initials: S
authors.lastname: Fukada
authors.affiliation: Translational Cardiomyology Laboratory, Embryo and Stem Cell Biology Unit, Dept of Development and Regeneration, KU Leuven, Belgium; Division of Human Anatomy, Dept of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy. Electronic address: maurilio.sampaolesi@med.kuleuven.be.
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D024510:Muscle Development / Q000235:genetics / Q000502:physiology*; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor / Q000235:genetics; D051761:Paired Box Transcription Factors / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C066855:HGF protein, human; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; C497322:Pax7 protein, mouse; D011994:Recombinant Proteins; C533045:magic-factor 1 protein, mouse; C068686:Pax3 protein, mouse; D049452:Green Fluorescent Proteins; D017228:Hepatocyte Growth Factor
keywords: Embryonic stem cells; Magic-F1; Mesoangioblast; Myogenic differentiation; Recombinant protein