title: Near infrared in vivo flow cytometry for tracking fluorescent circulating cells.
abstract: The in vivo flow cytometry (IVFC) is now a powerful technique in biomedical research, especially for tracking specific cells in circulatory system. The current fluorescence-based IVFC is limited to visible spectrum, while near infrared (NIR) dyes have their advantages, such as deeper penetration, less absorption and less scattering for NIR fluorescence. Here, using an NIR in vivo flow cytometer with a 785 nm laser excitation, the measurement of fluorescent dye IR-780 labeled circulating cells is demonstrated. Representative peaks corresponding to NIR fluorescent circulating cells are detected and quantified. In addition, blood flow information, including the blood flow velocity and flow volume per unit time, is obtained. By simultaneous detection of IR-780 and enhanced green fluorescent protein (EGFP) signals from dual labeled cells, the IR-780 is shown to be a suitable fluorescent dye for multicolor detection by IVFC, including NIR. Thus, the IVFC is extended to the NIR range and shows potential application in biomedical research.
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: Yuanzhen
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: Chengying
authors.initials: C
authors.lastname: Xie
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Tan
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Liao
authors.initials: L
authors.lastname: Wu
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wang
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.affiliation: Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu, 610209, China.|Chinese Academy of Sciences Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu, 610209, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Shi
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: Xunbin
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Chunmeng
authors.initials: C
authors.lastname: Shi
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D058948:Cell Tracking / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D049452:Green Fluorescent Proteins / Q000032:analysis*; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins
keywords: In vivo flow cytometry; circulating cells; near infrared fluorescent dyes; stem cells