title: Glial cell line-derived neurotrophic factor in combination with insulin-like growth factor 1 and basic fibroblast growth factor promote in vitro culture of goat spermatogonial stem cells.
abstract: Growth factors are increasingly considered as important regulators of spermatogonial stem cells (SSCs). This study investigated the effects of various growth factors (GDNF, IGF1, bFGF, EGF and GFRalpha-1) on purification and colonization of undifferentiated goat SSCs under in vitro and in vivo conditions. Irrespective of the culture condition used, the first signs of developing colonies were observed from day 4 of culture onwards. The number of colonies developed in GDNF + IGF1 + bFGF culture condition was significantly higher than the other groups (p < 0.05). In contrast, the size of colonies developed in GDNF + EGF + LIF culture condition was significantly higher than the other groups (p < 0.05). Immunocytochemical stationing for specific biomarkers of somatic cells (vimentin, alpha-inhibin and α-SMA) and spermatogonial cells (PLZF, THY 1, VASA, alpha-1 integrin, bet-1 integrin and DBA) revealed that both cell types existed in developing colonies, irrespective of the culture condition used. Even though, the relative abundance of VASA, FGFR3, OCT4, PLZF, BCL6B and THY1 transcription factors in GDNF + IGF1 + bFGF treatment group was significantly higher than the other groups (p < 0.05). Additionally, goat SSCs developed in the latter culture condition could colonize within the seminiferous tubules of the germ-cell depleted recipient mice following xenotransplantation. Obtained results demonstrated that combination of GDNF with IGF1 and bFGF promote in vitro culture of goat SSCs while precludes uncontrolled proliferation of somatic cells.
authors.affiliation: a Department of Biology , Falavarjan Branch, Islamic Azad University , Isfahan , Iran and.
authors.forename: M
authors.initials: M
authors.lastname: Bahadorani
authors.affiliation: b Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center , Royan Institute for Biotechnology , ACECR , Isfahan , Iran.
authors.forename: S M
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: b Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center , Royan Institute for Biotechnology , ACECR , Isfahan , Iran.
authors.forename: P
authors.initials: P
authors.lastname: Abedi
authors.affiliation: b Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center , Royan Institute for Biotechnology , ACECR , Isfahan , Iran.
authors.forename: H
authors.initials: H
authors.lastname: Abbasi
authors.affiliation: b Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center , Royan Institute for Biotechnology , ACECR , Isfahan , Iran.
authors.forename: M H
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology*; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000378:metabolism; D006041:Goats; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology; D009457:Neuroglia / Q000378:metabolism; D012671:Seminiferous Tubules / Q000166:cytology; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000166:cytology; D014157:Transcription Factors / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D009414:Nerve Growth Factors; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I
keywords: GDNF; IGF1; bFGF; goat; spermatogonia