title: MiR-499 Enhances the Cisplatin Sensitivity  of Esophageal Carcinoma Cell Lines by  Targeting DNA Polymerase β.
abstract: BACKGROUND
  Human DNA polymerase β (DNA polymerase β, polβ) is a small monomeric protein essential for short-patch base excision repair (BER). It plays an important role in regulating the sensitivity of tumor cells to chemotherapy.
  
  
  METHODS
  Luciferase reporter and western blot assays were used to determine whether polβ is a major target of miR-499. CCK-8, colony-forming survival and in vivo tumor growth assays were conducted to evaluate if miR- 499 can potentially enhance the cisplatin sensitivity and therefore inhibit the proliferation of esophageal cancer (EC) cells. Flow cytometry and immunofluorescence microscopy assays were performed to evaluate whether miR-499 enhance the cisplatin sensitivity and the corresponding apoptosis in EC cells.
  
  
  RESULTS
  polβ was pinpointed as a target gene of miR- 499. Additionally, we identified that miR-499 can enhance cisplatin's function of inhibiting proliferation and of promoting apoptosis in EC9706 and KYSE30 cell lines.
  
  
  CONCLUSIONS
  We first investigated whether miR-499 modulates polβ, and observed the influence of miR-499 up-regulation on the sensitivity of EC cell lines to cisplatin treatment. Our study paves the way for more insightful understanding and application of chemotherapy in esophageal cancer in the future.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianfang
authors.initials: J
authors.lastname: Feng
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Sun
authors.forename: Ziming
authors.initials: Z
authors.lastname: Dong
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001483:Base Sequence; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology* / Q000627:therapeutic use; D019951:DNA Polymerase beta / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D004947:Esophagus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D000970:Antineoplastic Agents; C548683:MIRN499 microRNA, human; D035683:MicroRNAs; D019951:DNA Polymerase beta; D002945:Cisplatin