title: High-Concentration Piperine: Capsaicin-Sensitive and -Insensitive Effects on Isolated Organs.
abstract: Piperine (P), a sensory stimulant in black pepper, is an agonist on TRPV1 receptors. Earlier work has showed capsaicin-sensitive and -insensitive mechanisms of the contractile action of P on the intestine. The current isolated organ study in the guinea-pig ileum, urinary bladder and trachea (a) confirms the presence of such components of effect (ileum and bladder); (b) indicates TRPV1 involvement in the effect of 5 or 30 µmol/l of P on the basis of an inhibitory action of the antagonist BCTC (ileum); (c) indicates that HC 030031-sensitive TRPA1 receptors and nifedipine-sensitive Ca(2+) channels contribute to the capsaicin-resistant contraction to 30 µmol/l P (ileum) and (d) shows that the contractile effect of P up to 100 µmol/l (guinea-pig trachea) or 30 µmol/l (guinea-pig urinary bladder) is capsaicin-sensitive and mediated by TRPV1 receptors/channels.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, University of Pécs Medical School, Pécs, Hungary.
authors.forename: Timea
authors.initials: T
authors.lastname: Bencsik
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Sandor
authors.forename: Lorand
authors.initials: L
authors.lastname: Bartho
mesh_terms: D000083:Acetanilides / Q000494:pharmacology; D000470:Alkaloids / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D052117:Benzodioxoles / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002211:Capsaicin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009543:Nifedipine / Q000494:pharmacology; D010880:Piperidines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D050916:TRPV Cation Channels / Q000819:agonists / Q000037:antagonists & inhibitors; D014132:Trachea / Q000187:drug effects*; D001743:Urinary Bladder / Q000187:drug effects*
chemical_list: C552888:2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide; D000083:Acetanilides; D000470:Alkaloids; D052117:Benzodioxoles; C475772:N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carboxamide; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D011687:Purines; D011719:Pyrazines; D011725:Pyridines; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D009543:Nifedipine; D002211:Capsaicin; C008922:piperine