title: Decrease in fluorescence lifetime by glycation of collagen and its application in determining advanced glycation end-products in human dentin.
abstract: Advanced Glycation End-products (AGEs) are produced by the Maillard reaction, which causes cross-linking of collagen and results in changes in the mechanical properties of collagen tissues. Several types of AGE fluoresce, and measurement of this fluorescence is effective for determining the presence of AGEs. Because fluorescence intensity by steady-state fluorometry is affected by sample surface condition and light source, we focused on fluorescence lifetime measurement (FLM). We found that fluorescence lifetime of collagen gel decreased with glycation progress. In vivo application of FLM for determination of AGEs was confirmed in human dentin.
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan ; These authors contributed equally to the work.
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Fukushima
authors.affiliation: Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Japan ; These authors contributed equally to the work.
authors.forename: Masato
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Miura
authors.affiliation: Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsuda
authors.affiliation: Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kubo
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Aoki
authors.affiliation: Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Takeshige
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
keywords: (000.1430) Biology and medicine; (030.5260) Photon counting; (170.1850) Dentistry; (170.2520) Fluorescence microscopy; (170.3650) Lifetime-based sensing