title: Molecular and serotyping characterization of shiga toxogenic Escherichia coli associated with food collected from Saudi Arabia.
abstract: Shiga toxin-producing Escherichia coli (STEC) strains are considered as one of the major food-borne disease agents in humans worldwide. STEC strains, also called verotoxin-producing E. coli strains. The objectives of the present study were serotyping and molecular characterization of shiga toxigenic E. coli associated with raw meat and milk samples collected from Riyadh, Saudi Arabia. A total of 540 milk samples were collected from 5 dairy farms and 150 raw meat samples were collected from different abattoirs located in Riyadh, Saudi Arabia. E. coli were recovered from 86 milk samples (15.93%), serotyping of E. coli isolates revealed, 26 (4.81%) strains O157: H7, 23 (4.26%) strains O111, 20 (3.70%) strains O113: H21, 10 (1.85%) strains O22: H8 and 7 (1.3%) strains O172: H21. Meanwhile, 17 (11.33%) strains of E. coli were recovered from raw meat samples, serotyping of E. coli isolates revealed, 6 (4%) strains O157: H7, 5 (3.33%) strains O111 and 4 (2.67%) strains O174: H2 and only two (1.33%) strains were identified as O22: H8. Shiga toxin2 was detected in 58 (67.44%) serotypes of E. coli recovered from milk samples and 16 (94.12%) serotypes of E. coli recovered from meat samples, while intimin gene was detected in 38 (44.186%) serotypes of E. coli recovered from milk samples and in 10 (58.82%) serotypes of E. coli recovered from meat samples. The results of this study revealed the efficiency of combination between serotyping and molecular typing of E. coli isolates recovered from food of animal origin for rapid detection and characterization of STEC.
authors.affiliation: Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
authors.forename: Onizan G
authors.initials: OG
authors.lastname: Al-Zogibi
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia ; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P.O. 2446, Cairo, 14242 Giza, Egypt.
authors.forename: Moussa I
authors.initials: MI
authors.lastname: Mohamed
authors.affiliation: Department of Health Science, College of Applied Studies and Community Service, King Saud University, P.O. Box 22459, Riyadh 11495, Saudi Arabia ; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P.O. 2446, Cairo, 14242 Giza, Egypt.
authors.forename: Ashgan M
authors.initials: AM
authors.lastname: Hessain
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P.O. 2446, Cairo, 14242 Giza, Egypt.
authors.forename: Jakeen K
authors.initials: JK
authors.lastname: El-Jakee
authors.affiliation: Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Kabli
keywords: E. coli; Foodborne; Intimin gene; Milk samples; Raw meat; Shiga toxin genes