title: Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria.
abstract: The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small-scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2, 2, 20, 200 and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e., increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role, since it explains the differences observed between the test performed at laboratory scale and in industrial conditions.
authors.affiliation: Groupe de Biochimie industrielle and Produits naturels, Université de Ton Duc Thang, 19 Nguyen Huu Tho, Tan Phong, Dis. 7, Ho Chi Minh Ville, Vietnam, nguyenhuuthanh@tdt.edu.vn.
authors.forename: Huu Thanh
authors.initials: HT
authors.lastname: Nguyen
authors.forename: Hary
authors.initials: H
authors.lastname: Razafindralambo
authors.forename: Aurore
authors.initials: A
authors.lastname: Richel
authors.forename: Nicolas
authors.initials: N
authors.lastname: Jacquet
authors.forename: Pol
authors.initials: P
authors.lastname: Evrard
authors.forename: Patrice
authors.initials: P
authors.lastname: Antoine
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
authors.forename: Frank
authors.initials: F
authors.lastname: Delvigne
mesh_terms: D001644:Bifidobacterium / Q000254:growth & development* / Q000378:metabolism; D019149:Bioreactors; D046508:Culture Techniques; D005612:Freeze Drying; D018869:Heat-Shock Response; D050296:Microbial Viability; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D019936:Probiotics; D013696:Temperature
chemical_list: D011135:Polysaccharides, Bacterial