title: Acid heteropolysaccharides with potent antileishmanial effects.
abstract: The present study investigated in vitro the effects of sulphated heterorhamnan (Go3), iota-/nu-carrageenans (G3d and EHW-I) and arabinogalactan (ARAGAL) polysaccharides on macrophage activation and inhibition of intracellular amastigotes of Leishmania (L.) amazonensis. All the sulphated polysaccharides (Go3, G3d and EHW-I) promoted increased nitric oxide production varying from 71 to 110%. The leishmanicidal activity of all compounds was compared to the inhibition effect of Meglumine Antimoniate at 300μg/mL (∼79%), used as positive control. Inhibition of Leishmania (L.) amazonensis growth was 55% with 5μg/mL of Go3, 50% and 98% to G3d and EHW-I, respectively at 10μg/mL, and 88% with 10μg/mL of ARAGAL. The superoxide anion scavenging activity for the sulphated polysaccharides varied from approximately 30-55% at 10μg/mL. In conclusion, the results of the present study indicate the promising potential of these polysaccharides for the development of new alternative therapeutic agents against leishmaniasis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Monica Mendes
authors.initials: MM
authors.lastname: Kangussu-Marcolino
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Marianna Maia Taulois
authors.initials: MM
authors.lastname: do Rosário
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Miguel Daniel
authors.initials: MD
authors.lastname: Noseda
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Maria Eugenia Rabello
authors.initials: ME
authors.lastname: Duarte
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Diogo Ricardo Bazan
authors.initials: DR
authors.lastname: Ducatti
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Juliana Emanuela Fogari
authors.initials: JE
authors.lastname: Cassolato
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil. Electronic address: iacomini@ufpr.br.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Glaucia Regina
authors.initials: GR
authors.lastname: Martinez
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Maria Eliane Merlin
authors.initials: ME
authors.lastname: Rocha
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Silvia Maria Suter Correia
authors.initials: SM
authors.lastname: Cadena
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Guilhermina Rodrigues
authors.initials: GR
authors.lastname: Noleto
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D007891:Leishmania / Q000187:drug effects* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D021261:Parasitic Sensitivity Tests; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D014344:Trypanocidal Agents / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; D014344:Trypanocidal Agents; D013481:Superoxides; D009569:Nitric Oxide
keywords: Leishmania; Macrophages; Polysaccharides