title: Distinct expression patterns of HCN channels in HL-1 cardiomyocytes.
abstract: BACKGROUND
  Cardiac rhythmic activity is initiated in functionally specialized areas of the heart. Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels are fundamental for these processes of cardiac physiology.
  
  
  RESULTS
  Here we investigated transcript and protein expression patterns of HCN channels in HL-1 cardiomyocytes using a combination of quantitative PCR analysis and immunocytochemistry. Gene expression profiles of hcn1, hcn2 and hcn4 were acutely affected during HL-1 cell propagation. In addition, distinct expression patterns were uncovered for HCN1, HCN2 and HCN4 proteins.
  
  
  CONCLUSIONS
  Our results suggest that HCN channel isoforms might be involved in the concerted differentiation of HL-1 cells and may indirectly affect the occurrence of contractile HL-1 cell activity. We expect that these findings will promote studies on other molecular markers that contribute to cardiac physiology.
authors.affiliation: Institute of Complex Systems, Cellular Biophysics (ICS-4), Wilhelm-Johnen-Straße, Forschungszentrum Jülich, D-52425, Jülich, Germany. a.guenther@fz-juelich.de.
authors.forename: Anne
authors.initials: A
authors.lastname: Günther
authors.affiliation: Institute of Complex Systems, Cellular Biophysics (ICS-4), Wilhelm-Johnen-Straße, Forschungszentrum Jülich, D-52425, Jülich, Germany. a.baumann@fz-juelich.de.
authors.forename: Arnd
authors.initials: A
authors.lastname: Baumann
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: C578666:Hcn1 protein, mouse; C578711:Hcn2 protein, mouse; C578706:Hcn4 protein, mouse; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D015221:Potassium Channels