title: Intron-based genomic editing: a highly efficient method for generating knockin zebrafish.
abstract: The TALEN and CRISPR/Cas9 nuclease systems have been extensively utilized in genomic engineering of model organisms. In zebrafish, the nuclease systems have been successfully applied in generating loss-of-function knockout lines. However, genome-specific knockin techniques in zebrafish are still at the beginning. In this perspective, we briefly summarize the recent progresses on knockin approaches in zebrafish with a special focus on the newly developed intron-based knockin method.
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.|University of Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Baibing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Bu
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.|School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China.|University of Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Jiulin
authors.initials: J
authors.lastname: Du
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D059766:DNA End-Joining Repair; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter; D005838:Genotype; D007438:Introns; D010641:Phenotype; D059767:Recombinational DNA Repair; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
keywords: CRISPR/Cas9; knockin; zebrafish