title: In Situ Proximity Ligation Assay (PLA).
abstract: In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.
authors.affiliation: Department of Neuroscience, Uppsala biomedicinska centrum BMC, Uppsala University, Husarg. 3, 593, Uppsala, 751 24, Sweden.
authors.forename: Sonchita
authors.initials: S
authors.lastname: Bagchi
authors.forename: Robert
authors.initials: R
authors.lastname: Fredriksson
authors.forename: Åsa
authors.initials: Å
authors.lastname: Wallén-Mackenzie
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D001923:Brain Chemistry; D005453:Fluorescence; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D057054:Molecular Imaging; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D025941:Protein Interaction Mapping; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D009419:Nerve Tissue Proteins; D009841:Oligonucleotides