title: Solid-phase extraction coupled with HPLC-DAD for determination of B vitamin concentrations in halophytes.
abstract: In our study, a simple and sensitive method was proposed for determination of vitamins B group in leaves of Suaeda aegyptiaca and Suaeda Vera (Halophytes) by HPLC-DAD with solid-phase extraction (SPE) as the preconcentration step. The variables affecting the extraction and determination conditions were investigated and optimized. A preconcentration step based on SPE is necessary to remove interfering components. The limit of detection obtained was <5 mg kg(-1), the relative standard deviation (RSD) was <4%, and the recovery was in the range of 91-102%. The linear correlation coefficient was 0.999. The method was successfully applied to determine group B vitamins in the halophyte samples. As a whole, these findings may confirm the interesting potential of this halophyte as a valuable source of natural bioactive molecules.
authors.affiliation: Abadan School of Medical Sciences, Abadan, Iran narges.chamkouri@gmail.com.
authors.forename: Narges
authors.initials: N
authors.lastname: Chamkouri
authors.affiliation: Department of Chemistry, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Khodadoust
authors.affiliation: Abadan School of Medical Sciences, Abadan, Iran.
authors.forename: Forogh
authors.initials: F
authors.lastname: Ghalavandi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D052616:Solid Phase Extraction / Q000379:methods*; D014803:Vitamin B Complex / Q000737:chemistry*
chemical_list: D014803:Vitamin B Complex