title: Detection of Marteilia refringens infecting the European flat oyster Ostrea edulis and the dwarf oyster Ostrea stentina in southern Portugal and Spain.
abstract: In the present study, Marteilia sp. was detected by histological examination and in situ hybridisation in Ostrea edulis and Ostrea stentina collected in southern Iberian Peninsula. Marteilia refringens DNA was detected by PCR in O. edulis (collected in southern Portugal) and O. stentina (collected in southern Spain and Portugal). Sequencing analysis revealed the presence of M. refringens type O in O. edulis, and type O and M in O. stentina. This is the first confirmed detection of M. refringens in Portugal and the first report on the occurrence of M. refringens infecting O. stentina in Europe.
authors.affiliation: IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3,8, 21459 Cartaya, Huelva, Spain. Electronic address: monserrats@yahoo.es.
authors.forename: Monserrat
authors.initials: M
authors.lastname: López-Sanmartín
authors.affiliation: Instituto Português do Mar e da Atmosfera, Divisão de Aquicultura e Valorização, Av. de Brasília, 1449-006 Lisboa, Portugal; Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Frederico M
authors.initials: FM
authors.lastname: Batista
authors.affiliation: IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3,8, 21459 Cartaya, Huelva, Spain.
authors.forename: María
authors.initials: M
authors.lastname: del Carmen Marín
authors.affiliation: IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3,8, 21459 Cartaya, Huelva, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Garrido
authors.affiliation: IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3,8, 21459 Cartaya, Huelva, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Quintero
authors.affiliation: Instituto Português do Mar e da Atmosfera, Divisão de Aquicultura e Valorização, Av. de Brasília, 1449-006 Lisboa, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Grade
authors.affiliation: Instituto Português do Mar e da Atmosfera, Divisão de Aquicultura e Valorização, Av. de Brasília, 1449-006 Lisboa, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Ruano
authors.affiliation: Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: de la Herrán
authors.affiliation: IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3,8, 21459 Cartaya, Huelva, Spain.
authors.forename: José I
authors.initials: JI
authors.lastname: Navas
mesh_terms: D000818:Animals; D056907:Cercozoa; D016054:DNA, Protozoan / Q000032:analysis; D006790:Host-Parasite Interactions; D017403:In Situ Hybridization; D049895:Ostrea / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D011174:Portugal; D013030:Spain
chemical_list: D016054:DNA, Protozoan
keywords: Histology; In situ hybridization; Marteilia refringens; Ostrea edulis; Ostrea stentina; PCR