title: [Analyses of Binding Profiles of the GII. 12 Norovirus with Human Histo-blood Group Antigens].
abstract: Interactions between noroviruses (NoVs) and the receptors of histo-blood group antigens (HB-GAs) affect the infectivity and host susceptibility of NoVs. We elucidated the binding profile of a GII. 12 NoV to HBGAs. First, we synthesized the P domain sequence of the GII. 12 NoV strain Pune (GenBank accession number EU921353). Protein of the P domain was expressed in a prokaryotic system. Formation of the P particle was monitored by gel-filtration chromatography. Antiserum was prepared by immunization of mice with GII. 12 P particles. The binding profile of the GII. 12 NoV Pune strain was determined by binding of the P particle with a panel of saliva samples with various known HBGAs phenotypes. The GII. 12 NoV was bound strongly to saliva samples of subjects with B and AB types and weakly to A, O secretor, and non-secretor saliva samples, suggesting higher affinity with B antigen by GII. 12 NoV. These results were consistent with those determined by a previous crystallography study of GII. 12 NoV. These data suggested that individuals with B and AB blood types may be more susceptible to infection by GII. 12 NoV compared with those with other blood types. Our findings may provide a basis for the prevention and control of an epidemic of GII. 12 NoV.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Kena
authors.initials: K
authors.lastname: Chen
authors.forename: Jingdong
authors.initials: J
authors.lastname: Song
authors.forename: Huiying
authors.initials: H
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Kong
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Duan
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000378:metabolism*; D017250:Caliciviridae Infections / Q000378:metabolism* / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000378:metabolism* / Q000821:virology*; D005838:Genotype; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D029322:Norovirus / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001789:Blood Group Antigens; D011991:Receptors, Virus; D014764:Viral Proteins