title: Induction of Angiogenesis by Matrigel Coating of VEGF-Loaded PEG/PCL-Based Hydrogel Scaffolds for hBMSC Transplantation.
abstract: hBMSCs are multipotent cells that are useful for tissue regeneration to treat degenerative diseases and others for their differentiation ability into chondrocytes, osteoblasts, adipocytes, hepatocytes and neuronal cells. In this study, biodegradable elastic hydrogels consisting of hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(ε-caprolactone) (PCL) scaffolds were evaluated for tissue engineering because of its biocompatibility and the ability to control the release of bioactive peptides. The primary cultured cells from human bone marrow are confirmed as hBMSC by immunohistochemical analysis. Mesenchymal stem cell markers (collagen type I, fibronectin, CD54, integrin1β, and Hu protein) were shown to be positive, while hematopoietic stem cell markers (CD14 and CD45) were shown to be negative. Three different hydrogel scaffolds with different block compositions (PEG:PCL=6:14 and 14:6 by weight) were fabricated using the salt leaching method. The hBMSCs were expanded, seeded on the scaffolds, and cultured up to 8 days under static conditions in Iscove's Modified Dulbecco's Media (IMDM). The growth of MSCs cultured on the hydrogel with PEG/PCL= 6/14 was faster than that of the others. In addition, the morphology of MSCs seemed to be normal and no cytotoxicity was found. The coating of the vascular endothelial growth factor (VEGF) containing scaffold with Matrigel slowed down the release of VEGF in vitro and promoted the angiogenesis when transplanted into BALB/c nude mice. These results suggest that hBMSCs can be supported by a biode gradable hydrogel scaffold for effective cell growth, and enhance the angiogenesis by Matrigel coating.
authors.affiliation: Department of Pharmacology and Medical Research Center, Ewha Womans University School of Medicine, Seoul 158-710, Korea.
authors.forename: Yeon Joo
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Heo
authors.affiliation: Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon 301-747, Korea.|Research Institute of Medical School, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Kaipeng
authors.initials: K
authors.lastname: Jing
authors.affiliation: Department of Pharmacology and Medical Research Center, Ewha Womans University School of Medicine, Seoul 158-710, Korea.
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Lee
authors.affiliation: Department of Pharmacology and Medical Research Center, Ewha Womans University School of Medicine, Seoul 158-710, Korea.
authors.forename: Jun Seok
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Kyu
authors.initials: K
authors.lastname: Lim
authors.affiliation: Division of Hematology/Oncology Department of Internal Medicine, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Deog-Yeon
authors.initials: DY
authors.lastname: Jo
authors.affiliation: Advanced Analysis Center, Korea Institute of Science and Technology, Seoul 136-791, Korea.
authors.forename: Jae Pyoung
authors.initials: JP
authors.lastname: Ahn
authors.affiliation: Department of Pathology, School of Medicine, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Polymer Science and Engineering, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Kang Moo
authors.initials: KM
authors.lastname: Huh
authors.affiliation: Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon 301-747, Korea.|Research Institute of Medical School, Chungnam National University, Daejeon 301-747, Korea.
authors.forename: Jong-Il
authors.initials: JI
authors.lastname: Park
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D017690:Cell Transplantation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism* / Q000633:toxicity; D004338:Drug Combinations; D006801:Humans; D020100:Hydrogels / Q000378:metabolism / Q000633:toxicity; D007797:Laminin / Q000378:metabolism* / Q000633:toxicity; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D011091:Polyesters / Q000378:metabolism* / Q000633:toxicity; D011092:Polyethylene Glycols / Q000378:metabolism* / Q000633:toxicity; D011509:Proteoglycans / Q000378:metabolism* / Q000633:toxicity; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D004338:Drug Combinations; D020100:Hydrogels; D007797:Laminin; D011091:Polyesters; D011509:Proteoglycans; D042461:Vascular Endothelial Growth Factor A; C059485:matrigel; C016240:polycaprolactone; D011092:Polyethylene Glycols; D003094:Collagen
keywords: VEGF; angiogenesis; controlled release; hBMSC; hydrogel