title: Noradrenaline modulates the presence of gonadotropin-releasing hormone in ovary. The importance of its interrelation on the ovarian steroidogenesis and apoptosis on dioestrus II in rat.
abstract: The aim of this work was to investigate if noradrenaline (NA), added in the coeliac ganglion -superior ovarian nerve- ovary system (CG-SON-O) and in ovary incubation, modifies the release of ovarian progesterone (P4), gonadotropin-releasing hormone (GnRH) and oestradiol (E2), and the expression of 3β-HSD and 20α-HSD and proapoptotic bax and antiapoptotic bcl-2 on dioestrus II in the rat. The CG-SON-O system and the ovary were removed and placed in one cuvette containing Krebs-Ringer solution (control groups), and NA was added to the ganglion compartment in the ex vivo system and in the ovary compartment in the ovary incubation (experimental groups). P4, GnRH and E2 were measured by RIA, and gene expression was measured by RT-PCR. In the ex-vivo system, the release of ovarian P4 and GnRH and the expression of 3β-HSD and bax decreased; E2 and bcl-2 increased, and the bax/bcl-2 ratio decreased. However, in the ovary incubation, P4, GnRH, the expression of 3β-HSD and bax increased; E2, the expression of 20α-HSD and bcl-2 decreased while the bax/bcl-2 ratio increased, thus favoring apoptosis. The peripheral nervous system protected the ovary from the apoptotic mechanisms while in the ovary incubation the effect was reverted. Our results indicate that NA regulates ovarian steroidogenesis and apoptosis by modulating GnRH release from the coeliac ganglion and ovary, being NA a possible generator of a GnRH-gonadotropins axis in the ovary. This work is expected to contribute with new evidence of the clinical importance of catecholamines and GnRH in therapy and prevention of ovarian pathologies.
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina; Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejercito de Los Andes 950- 1er Bloque 1er piso ala Norte D5700HHW, San Luis, República Argentina. Electronic address: cdbronzi@hotmail.com.
authors.forename: Cynthia D
authors.initials: CD
authors.lastname: Bronzi
authors.affiliation: Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejercito de Los Andes 950- 1er Bloque 1er piso ala Norte D5700HHW, San Luis, República Argentina.
authors.forename: Adriana S Vega
authors.initials: AS
authors.lastname: Orozco
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Rodriguez
authors.affiliation: Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejercito de Los Andes 950- 1er Bloque 1er piso ala Norte D5700HHW, San Luis, República Argentina.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Rastrilla
authors.affiliation: Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejercito de Los Andes 950- 1er Bloque 1er piso ala Norte D5700HHW, San Luis, República Argentina.
authors.forename: Zulema Y
authors.initials: ZY
authors.lastname: Sosa
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina; Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejercito de Los Andes 950- 1er Bloque 1er piso ala Norte D5700HHW, San Luis, República Argentina; Instituto de Biología y Medicina Experimental (IByME-CONICET), Argentina.
authors.forename: Marilina
authors.initials: M
authors.lastname: Casais
mesh_terms: D043607:20-alpha-Hydroxysteroid Dehydrogenase / Q000235:genetics; D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D015096:3-Hydroxysteroid Dehydrogenases; D043607:20-alpha-Hydroxysteroid Dehydrogenase; D009638:Norepinephrine
keywords: Apoptosis; Coeliac ganglion; GnRH; Noradrenaline; Ovary