title: Cy5.5 conjugated MnO nanoparticles for magnetic resonance/near-infrared fluorescence dual-modal imaging of brain gliomas.
abstract: The fusion of molecular and anatomical modalities facilitates more reliable and accurate detection of tumors. Herein, we prepared the PEG-Cy5.5 conjugated MnO nanoparticles (MnO-PEG-Cy5.5 NPs) with magnetic resonance (MR) and near-infrared fluorescence (NIRF) imaging modalities. The applicability of MnO-PEG-Cy5.5 NPs as a dual-modal (MR/NIRF) imaging nanoprobe for the detection of brain gliomas was investigated. In vivo MR contrast enhancement of the MnO-PEG-Cy5.5 nanoprobe in the tumor region was demonstrated. Meanwhile, whole-body NIRF imaging of glioma bearing nude mouse exhibited distinct tumor localization upon injection of MnO-PEG-Cy5.5 NPs. Moreover, ex vivo CLSM imaging of the brain slice hosting glioma indicated the preferential accumulation of MnO-PEG-Cy5.5 NPs in the glioma region. Our results therefore demonstrated the potential of MnO-PEG-Cy5.5 NPs as a dual-modal (MR/NIRF) imaging nanoprobe in improving the diagnostic efficacy by simultaneously providing anatomical information from deep inside the body and more sensitive information at the cellular level.
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shao
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, PR China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, PR China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Qu
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China. Electronic address: weigu@ccmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, PR China. Electronic address: cjyu1955@sina.com.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China. Electronic address: lingye@ccmu.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D002232:Carbocyanines / Q000008:administration & dosage / Q000737:chemistry*; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry; D005453:Fluorescence; D005910:Glioma / Q000175:diagnosis* / Q000473:pathology; D007259:Infrared Rays; D008279:Magnetic Resonance Imaging; D008297:Male; D017895:Manganese Compounds / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000175:diagnosis; D010087:Oxides / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry; D013499:Surface Properties
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D003287:Contrast Media; D017895:Manganese Compounds; D010087:Oxides; D011092:Polyethylene Glycols; C027424:manganese oxide
keywords: Brain gliomas; Cy5.5 conjugated MnO nanoparticles; Dual-modal nanoprobe; Magnetic resonance (MR) imaging; Near-infrared fluorescence (NIR) imaging