title: Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from grapevine cultivar Muscat Bailey A (Vitis labrusca × V. vinifera).
abstract: 2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape. Furaneol glucoside was recently isolated as an important furaneol derivative from the hybrid grapevine cultivar, Muscat Bailey A (V. labrusca × V. vinifera), and this was followed by its isolation from some fruits such as strawberry and tomato. Furaneol glucoside is a significant 'aroma precursor of wine' because furaneol is liberated from it during alcoholic fermentation. In this study, a glucosyltransferase gene from Muscat Bailey A (UGT85K14), which is responsible for the glucosylation of furaneol was identified. UGT85K14 was expressed in the representative grape cultivars regardless of species, indicating that furaneol glucoside content is regulated by the biosynthesis of furaneol. On the other hand, furaneol glucoside content in Muscat Bailey A berry during maturation might be controlled by the expression of UGT85K14 along with the biosynthesis of furaneol. Recombinant UGT85K14 expressed in Escherichia coli is able to transfer a glucose moiety from UDP-glucose to the hydroxy group of furaneol, indicating that this gene might be UDP-glucose: furaneol glucosyltransferase in Muscat Bailey A.
authors.affiliation: Laboratory, New Product & Process Developments, Mercian Corporation, 4-9-1 Johnan, Fujisawa, Kanagawa 251-0057, Japan sasaki-kn@mercian.co.jp.
authors.forename: Kanako
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Laboratory, New Product & Process Developments, Mercian Corporation, 4-9-1 Johnan, Fujisawa, Kanagawa 251-0057, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Takase
authors.affiliation: Château Mercian,1425-1 Shimoiwasaki, Katsunuma, Koshu, Yamanashi 409-1313, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Château Mercian,1425-1 Shimoiwasaki, Katsunuma, Koshu, Yamanashi 409-1313, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Matsuo
authors.affiliation: Laboratory, New Product & Process Developments, Mercian Corporation, 4-9-1 Johnan, Fujisawa, Kanagawa 251-0057, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Takata
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005663:Furans / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
chemical_list: D005663:Furans; D010940:Plant Proteins; D011994:Recombinant Proteins; C058427:furaneol; D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose
keywords: Furaneol; Muscat Bailey A; Vitis; glucosyltransferase; grape; wine