title: The ecdysone receptor coactivator Taiman links Yorkie to transcriptional control of germline stem cell factors in somatic tissue.
abstract: The Hippo pathway is a conserved signaling cascade that modulates tissue growth. Although its core elements are well defined, factors modulating Hippo transcriptional outputs remain elusive. Here we show that components of the steroid-responsive ecdysone (Ec) pathway modulate Hippo transcriptional effects in imaginal disc cells. The Ec receptor coactivator Taiman (Tai) interacts with the Hippo transcriptional coactivator Yorkie (Yki) and promotes expression of canonical Yki-responsive genes. Tai enhances Yki-driven growth, while Tai loss, or a form of Tai unable to bind Yki, suppresses Yki-driven tissue growth. This growth suppression is not correlated with impaired induction of canonical Hippo-responsive genes but with suppression of a distinct pro-growth program of Yki-induced/Tai-dependent genes, including the germline stem cell factors nanos and piwi. These data reveal Hippo/Ec pathway crosstalk in the form a Yki-Tai complex that collaboratively induces germline genes as part of a transcriptional program that is normally repressed in developing somatic epithelia.
authors.affiliation: Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Can
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Pathology & Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Robinson
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Yao
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA.
authors.forename: Heya
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Graduate Program in Genetics and Molecular Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Phil K
authors.initials: PK
authors.lastname: Byun
authors.affiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA.
authors.forename: Alexey
authors.initials: A
authors.lastname: Veraksa
authors.affiliation: Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: kmoberg@emory.edu.
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Moberg
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster; D004848:Epithelium / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D011987:Receptors, Steroid / Q000378:metabolism*; D019089:Stem Cell Factor; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000196:embryology; D000091102:YAP-Signaling Proteins; D000099317:Hippo Kinases
chemical_list: D060565:Argonaute Proteins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D011987:Receptors, Steroid; D019089:Stem Cell Factor; D015534:Trans-Activators; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C422002:TAI protein, Drosophila; C503814:Yki protein, Drosophila; C055469:ecdysone receptor; C116498:piwi protein, Drosophila; C070574:nos protein, Drosophila; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases