title: The type II histidine triad protein HtpsC is a novel adhesion with the involvement of Streptococcus suis virulence.
abstract: Streptococcal histidine triad proteins HTPs are widely distributed within the Streptococcus genus. Based on the phylogenetic relationship and domain composition, HTPs are classified into type I and type II subfamilies. Previous studies revealed that several pathogenic streptococci contain more than one htp gene. We found that the highly virulent strain of Streptococcus suis 2 (S. suis 2), 05ZYH33 encodes 3 HTPs, designated HtpsA (previously described as HtpS), HtpsB, and HtpsC. Among them, HtpsC is the only member that contains leucine-rich repeat (LRR) domains at the C-terminal. In this study, we demonstrated that the recombinant HtpsC could bind to 2 different components of human ECM complex laminin and fibronectin in vitro, suggesting that it is a novel adhesin of S. suis 2. Having constructed an htpsC mutant, we evaluated its role in the pathogenesis of the highly virulent S. suis 2 strain 05ZYH33. Our data showed that inactivation of htpsC significantly affected adherence of S. suis 2 to Hep-2 cells and shortened the survival of the bacteria in whole blood. Furthermore, deletion of htpsC significantly attenuated the virulence of S. suis 2 in mice. These results demonstrated that htpsC was involved in the pathogenesis of the highly virulent S. suis 2 strain 05ZYH33. In line with the observation, immunization with HtpsC significantly prolonged mice's survival after S. suis 05ZYH33 challenge, indicating its potential use in the vaccine development against S. suis.
authors.affiliation: a School of Laboratory Medicine and Life Science ; Wenzhou Medical University ; Wenzhou , China.|b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.|c State Key Laboratory of Pharmaceutical Biotechnology ; School of Life Sciences ; Nanjing University ; Nanjing , China.
authors.forename: Zhu-Qing
authors.initials: ZQ
authors.lastname: Shao
authors.affiliation: a School of Laboratory Medicine and Life Science ; Wenzhou Medical University ; Wenzhou , China.|b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.|d School of Life Sciences ; Nanjing Normal University ; Nanjing , China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Hou
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Hu
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zheng
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.|e Institute of Laboratory Medicine ; Jinling Hospital ; Nanjing , China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Tang
authors.affiliation: b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Wang
authors.affiliation: f Center for Infection & Immunity ; Department of Medical Microbiology & Parasitology ; Zhejiang University School of Medicine ; Hangzhou, Zhejiang , China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Feng
authors.affiliation: a School of Laboratory Medicine and Life Science ; Wenzhou Medical University ; Wenzhou , China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Gao
authors.affiliation: a School of Laboratory Medicine and Life Science ; Wenzhou Medical University ; Wenzhou , China.|b Department of Epidemiology ; Research Institute for Medicine of Nanjing Command ; Nanjing , China.
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Pan
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D001769:Blood / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D055786:Gene Knockout Techniques; D006801:Humans; D006867:Hydrolases / Q000172:deficiency / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D011485:Protein Binding; D013290:Streptococcal Infections / Q000517:prevention & control; D017011:Streptococcus suis / Q000502:physiology*; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D001428:Bacterial Vaccines; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; C097552:histidine triad protein; D006867:Hydrolases
keywords: Streptococcus suis serotype 2; histidine triad protein; virulence