title: A highly selective and sensitive ON-OFF-ON fluorescence chemosensor for cysteine detection in endoplasmic reticulum.
abstract: A new complex between coumarin-based ligand (CL) and copper ion has been prepared and applied to be an ON-OFF-ON reversible fluorescence chemosensor for the detection of Cys with high sensitivity and specificity. In HEPES buffer, CL displayed high affinity towards Cu(2+) ion over other physiological and environmental metal ions, accompanied with a 98.4% of fluorescence quenching. In the presence of Cys, the detachment of Cu(2+) ion of CL-Cu(2+) ensemble led to the liberation of the fluorophore, CL, and thus fluorescence was recovered. The results of absorption and emission spectroscopy analyses confirmed that the fluorescence turn ON response of CL-Cu(2+) ensemble was selective towards Cys only with high sensitivity (detection of limit, 7.2×10(-7) M). Confocal microscopy studies indicated that the lipophilic CL targets the endoplasmic reticulum (ER) of live cells, where it could be functioned as a fluorescent chemosensor for visualization of Cys in this organelle. Quantitative fluorescence detection of Cys in ER was successfully realized by flow cytometry analysis. The developed chemosensor was further applied to detect Cys in real urine samples with great recoveries ranges from 95.41% to 107.40%.
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, PR China.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, PR China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Jia
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Succar
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian High-Tech Industrial Zone, 116024, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia. Electronic address: run.zhang@mq.edu.au.
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian High-Tech Industrial Zone, 116024, PR China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Duan
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, PR China. Electronic address: zhangzhiqiang@ustl.edu.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D003300:Copper / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis*; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D015336:Molecular Probe Techniques / Q000295:instrumentation; D015335:Molecular Probes / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D003374:Coumarins; D015335:Molecular Probes; D003300:Copper; C030123:coumarin; D003545:Cysteine
keywords: Cell imaging; Coumarin; Cysteine; Endoplasmic reticulum; Fluorescence chemosensor; Quantification