title: Miniaturization of spectrophotometry based on micro flow analysis using norfloxacin as less-toxic reagent for iron determination.
abstract: A micro flow analysis (μFA) system has been designed and fabricated for determination of total iron. The system consists of a microchannels fabricated by etching the polymethyl methacrylate (PMMA) by using laser ablation techniques and a sealed polydimethylsiloxane (PDMS) as top plate. The PMMA micro-flow was topped with a home-made polydimethylsiloxane (PDMS) micro-flow through cell, which was integrated with light emitting diode (LED) as light source and a USB 2000 spectrometer as detector. The proposed μFA system was applied to determination of Fe(III) using norfloxacin as a less-toxic complexing agent in an acetate buffer solution pH 4.0, resulting in a yellow colored complex which gave the maximum absorption at 430nm. Under the optimum conditions, a linear calibration graph was obtained in the concentration range of 0.20-5.00mgL(-1). The limit of detection (LOD, defined as 3σ) and limit of quantification (LOQ, defined as 10σ) were 0.12 and 0.45mgL(-1), respectively. The relative standard deviation (R.S.D.) for repeatability and reproducibility were less than 1.50% and 1.24% (n=11) for 0.2mgL(-1) and 1.0mgL(-1) Fe(III), respectively. The proposed method was successfully applied to the determination of total iron in water samples, validated by the FAAS standard method after digestion by HNO3 and H2O2.
authors.affiliation: Department of Chemistry and Center of Excellent for Innovation in Chemistry (PERCH-CIC) Together with Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Kanyarak
authors.initials: K
authors.lastname: Prasertboonyai
authors.affiliation: Department of Chemistry and Center of Excellent for Innovation in Chemistry (PERCH-CIC) Together with Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Orn-Anong
authors.initials: OA
authors.lastname: Arqueropanyo
authors.affiliation: Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Boonsom
authors.initials: B
authors.lastname: Liawraungrath
authors.affiliation: Department of Chemistry and Center of Excellent for Innovation in Chemistry (PERCH-CIC) Together with Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Saisunee
authors.initials: S
authors.lastname: Liawraungrath
authors.affiliation: Department of Chemistry and Center of Excellent for Innovation in Chemistry (PERCH-CIC) Together with Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: teraboon2@gmail.com.
authors.forename: Teraboon
authors.initials: T
authors.lastname: Pojanakaroon
mesh_terms: D017022:Flow Injection Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis*; D008904:Miniaturization / Q000379:methods*; D009643:Norfloxacin / Q000737:chemistry*; D013053:Spectrophotometry; D059005:Topoisomerase II Inhibitors / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D059005:Topoisomerase II Inhibitors; D014874:Water Pollutants, Chemical; D007501:Iron; D009643:Norfloxacin
keywords: Iron(III); Micro flow analysis; Norfloxacin