title: Girdin/GIV is upregulated by cyclic tension, propagates mechanical signal transduction, and is required for the cellular proliferation and migration of MG-63 cells.
abstract: To explore how Girdin/GIV is regulated by cyclic tension and propagates downstream signals to affect cell proliferation and migration. Human osteoblast-like MG-63 cells were exposed to cyclic tension force at 4000 μstrain and 0.5 Hz for 6 h, produced by a four-point bending system. Cyclic tension force upregulated Girdin and Akt expression and phosphorylation in cultured MG-63 cells. Girdin and Akt each promoted the phosphorylation of the other under stimulated tension. In vitro MTT and transwell assays showed that Girdin and Akt are required for cell proliferation and migration during cellular quiescence. Moreover, STAT3 was determined to be essential for Girdin expression under stimulated tension force in the physiological condition, as well as for osteoblast proliferation and migration during quiescence. These findings suggest that the STAT3/Girdin/Akt pathway activates in osteoblasts in response to mechanical stimulation and may play a significant role in triggering osteoblast proliferation and migration during orthodontic treatment.
authors.affiliation: Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Jiang-Tian
authors.initials: JT
authors.lastname: Hu
authors.affiliation: Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Guo-Jie
authors.initials: GJ
authors.lastname: Gao
authors.affiliation: Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: song_li59@126.com.
authors.forename: Song
authors.initials: S
authors.lastname: Li
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D006801:Humans; D008840:Microfilament Proteins / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation; D033921:Vesicular Transport Proteins / Q000502:physiology*
chemical_list: C502451:CCDC88A protein, human; D008840:Microfilament Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D033921:Vesicular Transport Proteins; D051057:Proto-Oncogene Proteins c-akt
keywords: Cell migration assays; Cell proliferation; Cellular mechanotransduction; Human Girdin protein; Osteoblasts