title: Antibiotic Resistance of Campylobacter jejuni and C. coli Isolated from Children with Diarrhea in Thailand and Japan.
abstract: A total of 29 Campylobacter jejuni and C. coli strains were isolated from Thai and Japanese children with diarrhea using the Loop-mediated Isothermal Amplification method. The samples were evaluated for mutations in gyrA and 23S rRNA in order to assess resistance against fluoroquinolones and macrolides, respectively. Among the isolated strains, 9 (8 C. jejuni and 1 C. coli) were from Thai children, and the other 20 (C. jejuni) were isolated from Japanese children. High fluoroquinolone resistance rates were observed in Thai (66.7%) and Japanese (90%) children. Macrolide resistance was not observed in Japanese children but was observed at a considerable rate of 12.5% of C. jejuni isolated in the Thai cohort. The results indicate that continuous monitoring of resistance of Campylobacter strains to fluoroquinolones and macrolides is definitely necessary.
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.
authors.forename: Ngan Thi Kim
authors.initials: NT
authors.lastname: Pham
authors.forename: Aksara
authors.initials: A
authors.lastname: Thongprachum
authors.forename: Dinh Nguyen
authors.initials: DN
authors.lastname: Tran
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nishimura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shimizu-Onda
authors.forename: Quang Duy
authors.initials: QD
authors.lastname: Trinh
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Nuthapong
authors.initials: N
authors.lastname: Ukarapol
authors.forename: Tipachan
authors.initials: T
authors.lastname: Kongsricharoern
authors.forename: Shihoko
authors.initials: S
authors.lastname: Komine-Aizawa
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D017000:Campylobacter coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D027081:DNA Gyrase / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D018942:Macrolides / Q000494:pharmacology; D009154:Mutation; D015995:Prevalence; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013785:Thailand / Q000453:epidemiology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D018942:Macrolides; D012338:RNA, Ribosomal, 23S; D027081:DNA Gyrase