title: Phosphoproteomics technologies and applications in plant biology research.
abstract: Protein phosphorylation has long been recognized as an essential mechanism to regulate many important processes of plant life. However, studies on phosphorylation mediated signaling events in plants are challenged with low stoichiometry and dynamic nature of phosphorylated proteins. Significant advances in mass spectrometry based phosphoproteomics have taken place in recent decade, including phosphoprotein/phosphopeptide enrichment, detection and quantification, and phosphorylation site localization. This review describes a variety of separation and enrichment methods for phosphoproteins and phosphopeptides, the applications of technological innovations in plant phosphoproteomics, and highlights significant achievement of phosphoproteomics in the areas of plant signal transduction, growth and development.
authors.affiliation: College of Life Sciences, Heilongjiang University Harbin, China.
authors.forename: Jinna
authors.initials: J
authors.lastname: Li
authors.affiliation: Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida Gainesville, FL, USA.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Silva-Sanchez
authors.affiliation: Plant Molecular and Cellular Biology Program, Department of Biology, UF Genetics Institute, University of Florida Gainesville, FL, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Heilongjiang University Harbin, China ; Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida Gainesville, FL, USA ; Plant Molecular and Cellular Biology Program, Department of Biology, UF Genetics Institute, University of Florida Gainesville, FL, USA.
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Heilongjiang University Harbin, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
keywords: enrichment; phosphoproteomics; phosphorylation site mapping; plant biology; quantification