title: Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats.
abstract: BACKGROUND
  Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are less invasive than bone marrow mesenchymal stem cells to obtain for cell therapy.
  
  
  OBJECTIVE
  The aims of this study were to evaluate the germinal cells characteristics and repairs in seminiferous tubules of busulfan-induced azoospermic rats after AT-MSCs transplantation.
  
  
  METHODS
  Experimental case-control study.
  
  
  METHODS
  In the present experimental study, donors AT-MSCs were isolated from subcutaneous adipose tissue of two Sprague-Dawley rats. The recipients (n = 5) were received two doses of 10 mg/kg of busulfan with 21 days interval to stop endogenous spermatogenesis. After induction of azoospermia by busulfan, rats were injected with the AT-MSCs into the efferent duct of right testes. After 60 days, the right testes were injected AT-MSCs were compared to left azoospermic testes. Five untreated male rats served as negative control.
  
  
  METHODS
  Stereological indices were analyzed by one-way ANOVA and LSD post-hoc test. The spermatogenesis index was compared using Mann-Whitney U test.
  
  
  RESULTS
  After stereological analyses, the seminiferous tubules treated with AT-MSCs had normal morphology. The untreated seminiferous tubules were empty. Spermatogenesis was observed in most cell-treated seminiferous tubules.
  
  
  CONCLUSIONS
  The testis of busulfan-induced azoospermic rats accepted transplanted AT-MSCs. The transplanted AT-MSCs could induce spermatogenesis in seminiferous tubules of the rat.
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Hassanshahi
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Tamadon
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Zare
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Saeideh
authors.initials: S
authors.lastname: Keshavarz
authors.affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Rahmanifar
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dianatpour
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khodabandeh
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: ImanRazeghian
authors.initials: I
authors.lastname: Jahromi
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Tanideh
authors.affiliation: Hematology and Bone Marrow Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mani
authors.initials: M
authors.lastname: Ramzi
authors.affiliation: Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.
authors.forename: Heydar
authors.initials: H
authors.lastname: Aqababa
authors.affiliation: Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Kuhi-Hoseinabadi
keywords: Adipose tissue; azoospermia; busulfan; cell therapy; mesenchymal stem cell; rat