title: Comparison of three media for transport and storage of the samples collected for detection of avian influenza virus.
abstract: Detection of avian influenza viruses (AIVs) is important for diagnosis, surveillance and control of avian influenza which is of great economic and public health significance. Proper transport and storage of samples is critical for the detection when the samples cannot be detected immediately. As recommended by some international or national authoritative entities and some publications, phosphate buffered saline (PBS), PBS-glycerol and brain heart infusion broth (BHIB) are frequently used for transport and storage of the samples collected for detection of AIVs worldwide. In this study, we compared these three media for transport and storage of simulated and authentic swab and feces samples collected for detection of AIVs using virus isolation and reverse transcription-PCR. The results suggest that PBS-glycerol is superior to PBS and BHIB as the sample transport and storage media. The results also suggest that the samples collected for detection of AIVs should be detected as soon as possible because the virus concentration of the samples may decline rapidly during storage within days at 4 or -20°C.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Zhang
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Liu
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Hou
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Qing-Ye
authors.initials: QY
authors.lastname: Zhuang
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Kai-Cheng
authors.initials: KC
authors.lastname: Wang
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Jiang
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Su-Chun
authors.initials: SC
authors.lastname: Wang
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Li
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Yu
authors.affiliation: China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Du
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; China Animal Health & Epidemiology Center, Qingdao 266032, China.
authors.forename: Bao-Xu
authors.initials: BX
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: jmchen678@qq.com.
authors.forename: Ji-Ming
authors.initials: JM
authors.lastname: Chen
mesh_terms: D000818:Animals; D001717:Birds; D002021:Buffers; D003470:Culture Media / Q000737:chemistry*; D009980:Influenza A virus / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D050296:Microbial Viability; D011200:Poultry; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D002021:Buffers; D003470:Culture Media
keywords: Avian influenza virus; Detection; Medium; Sample; Storage