title: Glutathione Supplementation Attenuates Oxidative Stress and Improves Vascular Hyporesponsiveness in Experimental Obstructive Jaundice.
abstract: We investigated the protective effects and mechanism of glutathione (GSH) on vascular hyporesponsiveness induced by bile duct ligation (BDL) in a rat model. Seventy-two male Sprague-Dawley rats were randomly divided into four groups: a NS group, a GSH group, a BDL + NS group, and a BDL + GSH group. GSH was administrated into rats in the GSH and BDL + GSH groups by gastric gavage. An equal volume of normal saline was, respectively, given in the NS group and BDL + NS group. Blood was gathered for serological determination and thoracic aorta rings were isolated for measurement of isometric tension. Obstructive jaundice led to a significant increase in the serum total bilirubin, AST, and ALT levels. The proinflammatory cytokines levels (TNF-α and IL-1β), concentration of NO, and oxidative stress markers (MDA and 3-NT) were increased as well. All of those were reduced by the treatment of GSH. Meanwhile, contraction of aorta rings to NA and vasorelaxation to ACh or SNP in the BDL group rats were markedly decreased, while GSH administration reversed this change. Our findings suggested that GSH supplementation attenuated overexpressed ONOO(-) from the reaction of excessive NO with O2 (∙-) and protected against obstructive jaundice-induced vascular hyporesponsiveness in rats.
authors.affiliation: Department of Anesthesia & Intensive Care, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China ; Department of Anesthesiology, 81st Hospital of the Chinese PLA, Nanjing 210002, China.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Anesthesia & Intensive Care, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China.
authors.forename: Feixiang
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Anesthesia & Intensive Care, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Long
authors.affiliation: Department of Anesthesia & Intensive Care, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001652:Bile Ducts / Q000293:injuries; D001663:Bilirubin / Q000097:blood; D005978:Glutathione / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D041781:Jaundice, Obstructive / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D053583:Interleukin-1beta; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D001663:Bilirubin