title: De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil.
abstract: BACKGROUND
  The blue crab, Callinectes sapidus, is economically and ecologically important in western Atlantic and Gulf of Mexico coastal estuaries. In 2010 blue crabs in the northern Gulf of Mexico were exposed to crude oil and chemical dispersants from the Deepwater Horizon oil spill. To characterize the blue crab transcriptome and identify genes that could be regulated in response to oil exposure we sequenced transcriptomes from hepatopancreas and gill tissues of juvenile blue crabs after exposing them to a water-accommodated fraction of surrogate Macondo crude oil in the laboratory and compared them to transcriptomes from an unexposed control group.
  
  
  RESULTS
  Illumina sequencing provided 42.5 million paired-end sequencing reads for the control group and 44.9 million paired-end reads for the treatment group. From these, 73,473 transcripts and 52,663 genes were assembled. Comparison of control and treatment transcriptomes revealed about 100 genes from each tissue type that were differentially expressed. However, a much larger number of transcripts, approximately 2000 from each tissue type, were differentially expressed. Several examples of alternatively spliced transcripts were verified by qPCR, some of which showed significantly different expression patterns. The combined transcriptome from all tissues and individuals was annotated to assign putative gene products to both major gene ontology categories as well as specific roles in responses to cold and heat, metabolism of xenobiotic compounds, defence, hypoxia, osmoregulation and ecdysis. Among the annotations for upregulated and alternatively-spliced genes were candidates for the metabolism of oil-derived compounds.
  
  
  CONCLUSIONS
  Previously, few genomic resources were available for blue crabs or related brachyuran crabs. The transcriptome sequences reported here represent a major new resource for research on the biology of blue crabs. These sequences can be used for studies of differential gene expression or as a source of genetic markers. Genes identified and annotated in this study include candidates for responses of the blue crab to xenobiotic compounds, which could serve as biomarkers for oil exposure. Changes in gene expression also suggest other physiological changes that may occur as the result of exposure to oil.
authors.affiliation: South Slough National Estuarine Research Reserve, Charleston, OR, USA. breeyednock@gmail.com.
authors.forename: Bree K
authors.initials: BK
authors.lastname: Yednock
authors.affiliation: Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA. tsulli1998@gmail.com.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Sullivan
authors.affiliation: Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA. jneigel_ull@agelus.net.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Neigel
mesh_terms: D000818:Animals; D003386:Brachyura / Q000235:genetics*; D063366:Estuaries; D008800:Mexico; D010578:Petroleum / Q000009:adverse effects*; D059392:Petroleum Pollution; D059467:Transcriptome / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D010578:Petroleum; D014874:Water Pollutants, Chemical