title: Metabolic faecal fingerprinting of trans-resveratrol and quercetin following a high-fat sucrose dietary model using liquid chromatography coupled to high-resolution mass spectrometry.
abstract: Faecal non-targeted metabolomics deciphers metabolic end-products resulting from the interactions among food, host genetics, and gut microbiota. Faeces from Wistar rats fed a high-fat sucrose (HFS) diet supplemented with trans-resveratrol and quercetin (separately or combined) were analysed by liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Metabolomics in faeces are categorised into four clusters based on the type of treatment. Tentative identification of significantly differing metabolites highlighted the presence of carbohydrate derivatives or conjugates (3-phenylpropyl glucosinolate and dTDP-D-mycaminose) in the quercetin group. The trans-resveratrol group was differentiated by compounds related to nucleotides (uridine monophosphate and 2,4-dioxotetrahydropyrimidine D-ribonucleotide). Marked associations between bacterial species (Clostridium genus) and the amount of some metabolites were identified. Moreover, trans-resveratrol and resveratrol-derived microbial metabolites (dihydroresveratrol and lunularin) were also identified. Accordingly, this study confirms the usefulness of omics-based techniques to discriminate individuals depending on the physiological effect of food constituents and represents an interesting tool to assess the impact of future personalized therapies.
authors.affiliation: Department of Nutrition, Food Science and Physiology, University of Navarra, C/Irunlarrea 1, 31008 Pamplona, Spain. jalfmtz@unav.es.
authors.forename: Usune
authors.initials: U
authors.lastname: Etxeberria
authors.forename: Noemi
authors.initials: N
authors.lastname: Arias
authors.forename: Noemí
authors.initials: N
authors.lastname: Boqué
authors.forename: Ana
authors.initials: A
authors.lastname: Romo-Hualde
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Macarulla
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
authors.forename: Fermín I
authors.initials: FI
authors.lastname: Milagro
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004041:Dietary Fats / Q000378:metabolism*; D019422:Dietary Sucrose / Q000378:metabolism*; D019587:Dietary Supplements; D005243:Feces / Q000737:chemistry*; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D011794:Quercetin / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D019422:Dietary Sucrose; D013267:Stilbenes; D011794:Quercetin; D000077185:Resveratrol