title: Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach.
abstract: Despite the recent advances in diagnostic and therapeutic strategies, oral squamous cell carcinoma (OSCC) remains a major health burden. Protein biomarker discovery for early detection will help to improve patient survival rate in OSCC. Mass spectrometry-based proteomics has emerged as an excellent approach for detection of protein biomarkers in various types of cancers. In the current study, we have used 4-Plex isobaric tags for relative and absolute quantitation (iTRAQ)-based shotgun quantitative proteomic approach to identify proteins that are differentially expressed in cancerous tissues compared to normal tissues. The high-resolution mass spectrometric analysis resulted in identifying 2,074 proteins, among which 288 proteins were differentially expressed. Further, it was noticed that 162 proteins were upregulated, while 125 proteins were downregulated in OSCC-derived cancer tissue samples as compared to the adjacent normal tissues. We identified some of the known molecules which were reported earlier in OSCC such as MMP-9 (8.4-fold), ZNF142 (5.6-fold), and S100A7 (3.5-fold). Apart from this, we have also identified some novel signature proteins which have not been reported earlier in OSCC including ras-related protein Rab-2A isoform, RAB2A (4.6-fold), and peroxiredoxin-1, PRDX1 (2.2-fold). The immunohistochemistry-based validation using tissue microarray slides in OSCC revealed overexpression of the RAB2A and PRDX1 gene in 80 and 68 % of the tested clinical cases, respectively. This study will not only serve as a resource of candidate biomarkers but will contribute towards the existing knowledge on the role of the candidate molecules towards disease progression and therapeutic potential.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. kaushik1011@gmail.com.
authors.forename: Kaushik Kumar
authors.initials: KK
authors.lastname: Dey
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. ipsita.pal1984@gmail.com.
authors.forename: Ipsita
authors.initials: I
authors.lastname: Pal
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. rashmi21bharti@gmail.com.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Bharti
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. goutamju24@gmail.com.
authors.forename: Goutam
authors.initials: G
authors.lastname: Dey
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. prasanthkumar999@gmail.com.
authors.forename: B N Prashanth
authors.initials: BN
authors.lastname: Kumar
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. shashirajput23@gmail.com.
authors.forename: Shashi
authors.initials: S
authors.lastname: Rajput
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. adityaparekh101@gmail.com.
authors.forename: Aditya
authors.initials: A
authors.lastname: Parekh
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. sparida020@gmail.com.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Parida
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. priyankahalder2005@yahoo.co.in.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Halder
authors.affiliation: Bankura Sammilani Medical College, Bankura, West Bengal, 722101, India. ikulavi2008@yahoo.com.
authors.forename: Indranil
authors.initials: I
authors.lastname: Kulavi
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India. mahitosh@smst.iitkgp.ernet.in.
authors.forename: Mahitosh
authors.initials: M
authors.lastname: Mandal
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D054464:Peroxiredoxins / Q000096:biosynthesis* / Q000235:genetics; D020543:Proteome / Q000235:genetics; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome; C515940:PRDX1 protein, human; D054464:Peroxiredoxins; D020691:rab GTP-Binding Proteins
keywords: OSCC; Oral squamous cell carcinoma; PRDX1; RAB2A; iTRAQ