title: Expression of various protection of telomeres 1 variants is associated with telomere length and radiosensitivity in colon and gastric adenocarcinoma cellsin vitro.
abstract: Protection of telomeres 1 (POT1) is a telomere-binding protein, which binds to the single-stranded DNA extensions of telomeres and regulates telomere length. DifferentPOT1mRNA variants were examined and compared with telomere length and radiosensitivity in colon and gastric adenocarcinoma cells.POT1production and telomere lengths were assessed using 10 human cancer cell lines by quantitative polymerase chain reaction (qPCR).POT1mRNA levels, which were relatively stable, were significantly correlated with telomere length in gastric cancer cells and colon cancer cells, except for HT29 (P<0.01).POT1v5 indexes were closely associated with radiosensitivity in colon cancer cells and gastric cancer cells (P<0.05). In conclusion,POT1may be a good marker for the examination of cell-specific telomere length and radiosensitivity.
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China ; Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China ; Hubei Clinical Cancer Study Center, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Fu-Xiang
authors.initials: FX
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Peng
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Zhang
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Wang
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China ; Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Yu
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China ; Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Cong-Hua
authors.initials: CH
authors.lastname: Xie
authors.affiliation: Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China ; Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China ; Hubei Clinical Cancer Study Center, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Zhou
keywords: chromosome protection; protection of telomeres 1 variant; radiosensitivity; telomere length