title: Antibacterial monoterpenoid indole alkaloids from Alstonia scholaris cultivated in temperate zone.
abstract: Three new monoterpenoid indole alkaloids, named normavacurine-21-one (1), 5-hydroxy-19, 20-E-alschomine (2), and 5-hydroxy-19, 20-Z-alschomine (3), together with thirteen known indole alkaloids (4-16) were isolated from the leaves of Alstonia scholaris cultivated in Kunming. Their structures were elucidated on the basis of extensive spectroscopic analysis, as well as by comparison with the reported spectroscopic data. The leaves of A. scholaris cultivated in Kunming, contained picrinine-type alkaloids, scholaricin-type alkaloids and nareline as major alkaloids. New compounds 1-3 might be derived from a common biogenetic precursor (5). Compounds 1, 5 and 10 exhibited significant antibacterial activity against Enterococcus faecalis, and 3, 9 and 14 against Pseudomonas aeruginosa with an MIC value of 0.781 μg/mL, while 14 showed moderate activity against Klepsiella pneumonia with an MIC value of 1.56 μg/mL.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China; University of Chinese Academy of Science, Beijing 100049, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China; University of Chinese Academy of Science, Beijing 100049, People's Republic of China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China; University of Chinese Academy of Science, Beijing 100049, People's Republic of China.
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China; University of Chinese Academy of Science, Beijing 100049, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China; University of Chinese Academy of Science, Beijing 100049, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China. Electronic address: liuyaping@mail.kib.ac.cn.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming institute of botany, Chinese Academy of Science, Kunming 650201, People's Republic of China. Electronic address: xdluo@mail.kib.ac.cn.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Luo
mesh_terms: D031051:Alstonia / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D046948:Secologanin Tryptamine Alkaloids
keywords: Alstonia scholaris; Antibacterial activity; Apocynaceae; Monoterpenoid indole alkaloids