title: Age-related decrease in mtDNA content as a consequence of mtDNA 4977 bp deletion.
abstract: As one of the most frequent somatic mutations accumulated during aging in human mitochondrial DNA, the 4977 bp deletion has intrigued scientific interest in recent years. Although many studies have shown a significant increase in the amount of 4977 bp deletion, the findings with respect to an age-dependent escalate of ΔmtDNA4977 bp in blood are still disputatious. Therefore, we investigated the presence of common deletion and mtDNA deletion level in whole blood samples of 100 old individuals (60-90 years). We detected the accumulation of common deletion in 46 old individuals. Consequently, there was statistically significant difference between the aged and young individuals in mitochondrial content (p = 0.01) and deletion levels ranged from 2% to 17% of the total mtDNA (mean: 10% ± 0.02%). We conclude that common deletion has decreased the mtDNA content; however, it is not clearly detectable in the blood as one of the fast replicating tissues comparing with tissues with low mitotic activity.
authors.affiliation: a Department of Biochemistry and Clinical Laboratory , Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran and.
authors.forename: Leila
authors.initials: L
authors.lastname: Zabihi Diba
authors.affiliation: a Department of Biochemistry and Clinical Laboratory , Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran and.
authors.forename: Seyed Mojtaba
authors.initials: SM
authors.lastname: Mohaddes Ardebili
authors.affiliation: a Department of Biochemistry and Clinical Laboratory , Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran and.
authors.forename: Jalal
authors.initials: J
authors.lastname: Gharesouran
authors.affiliation: b Department of Medical Genetics , National Institute for Genetic Engineering and Biotechnology , Tehran , Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Houshmand
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics*; D004272:DNA, Mitochondrial; D054629:Genome, Mitochondrial; D006801:Humans; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
chemical_list: D004272:DNA, Mitochondrial
keywords: Aging; common deletion; mitochondria; mtDNA deletion; ΔmtDNA 4977 bp