title: Evaluation of a new rapid diagnostic test for the detection of influenza and RSV.
abstract: BACKGROUND
  Influenza viruses and respiratory syncytial virus (RSV) can cause an acute respiratory disease that occurs seasonally in epidemic waves. This retrospective study was conducted to evaluate the Sofia(®) Influenza A+B and the Sofia(®) RSV fluorescence immunoassays (FIAs), two novel rapid detection tests (RDTs) for influenza A and B and RSV.
  
  
  METHODS
  Two hundred and nine breath samples were selected from patients with respiratory symptoms determined to be positive/negative for influenza A, influenza B or RSV using one of the reference diagnostic techniques, cell culture and/or RT-PCR (Simplexa™Flu A/B & RSV). The Sofia Influenza A+B FIA was tested on 123 samples (63 from children and 60 from adults) and the Sofia RSV FIA was tested on 86 pediatric samples. Sensitivity and specificity values of both assays were calculated assuming the reference techniques as the gold standard.
  
  
  RESULTS
  Sensitivity and specificity values for the Sofia Influenza A+B FIA were 73.1% and 97.8%, respectively. Sensitivity and specificity values for the Sofia RSV FIA were 87.5% and 86.7%, respectively.
  
  
  CONCLUSIONS
  The sensitivity results obtained for the two assays were considerably higher than those reported for other RDTs. In conclusion, the Sofia Influenza A+B and the Sofia RSV FIAs are appropriate tools for the rapid diagnosis of these viruses.
authors.affiliation: Laboratorio de Virología, Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, Spain. Electronic address: microsaragg@gmail.com.
authors.forename: Sara
authors.initials: S
authors.lastname: Gómez
authors.affiliation: Laboratorio de Virología, Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Columbiana
authors.initials: C
authors.lastname: Prieto
authors.affiliation: Laboratorio de Virología, Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Vera
authors.affiliation: Laboratorio de Virología, Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: R Otero
authors.affiliation: Laboratorio de Virología, Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Lola
authors.initials: L
authors.lastname: Folgueira
mesh_terms: D001944:Breath Tests; D002460:Cell Line; D003955:Diagnostic Tests, Routine; D006801:Humans; D009980:Influenza A virus; D009981:Influenza B virus; D007251:Influenza, Human / Q000175:diagnosis*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D012136:Respiratory Syncytial Viruses; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
keywords: Immunofluorescence; Infección respiratoria; Influenza viruses; Inmunofluorescencia; RSV; Rapid diagnostic tests; Respiratory infection; Test rápido; VRS; Virus influenza