title: Mechanical clearance of red blood cells by the human spleen: Potential therapeutic applications of a biomimetic RBC filtration method.
abstract: During their lifespan, circulating RBC are frequently checked for their deformability. This mechanical quality control operates essentially in the human spleen. RBC unable to squeeze though narrow splenic slits are retained and cleared from the blood circulation. Under physiological conditions this prevents microvessels from being clogged by senescent, rigid RBC. Retention of poorly deformable RBC is an important determinant of pathogenesis in malaria and may also impact the clinical benefit of transfusion. Modulating the splenic retention of RBC has already been proposed to support therapeutic approaches in these research fields. To this aim, the development of microplates for high throughput filtration of RBC through microsphere layers (microplate-based microsphiltration) has been undertaken. This review focuses on potential therapeutic applications provided by this technology in malaria chemotherapy and transfusion.
authors.affiliation: CIMI-Paris U1135, équipe 4, hôpital La Pitié-Salpêtrière, 75013 Paris, France; Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France; HRA Pharma Laboratoires, 15, rue de Béranger, 75003 Paris, France; Eskitis Institute for Drug Discovery, Griffith University, Brisbane Innovation Park, Don Young Road, Nathan, QLD 4111, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Duez
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane Innovation Park, Don Young Road, Nathan, QLD 4111, Australia.
authors.forename: J P
authors.initials: JP
authors.lastname: Holleran
authors.affiliation: CIMI-Paris U1135, équipe 4, hôpital La Pitié-Salpêtrière, 75013 Paris, France; Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France.
authors.forename: P A
authors.initials: PA
authors.lastname: Ndour
authors.affiliation: CIMI-Paris Plateforme post-génomique de la Pitié Salpêtrière, P3S, hôpital La Pitié-Salpêtrière, 75013 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Pionneau
authors.affiliation: CIMI-Paris U1135, équipe 4, hôpital La Pitié-Salpêtrière, 75013 Paris, France; Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Diakité
authors.affiliation: Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France; Inserm U1163/CNRS ERL 8254, Imagine Institute, Paris Descartes-Sorbonne Paris Cité University, 75015 Paris, France; Institut national de la transfusion sanguine (INTS), 75015 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Roussel
authors.affiliation: Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France; Inserm U1163/CNRS ERL 8254, Imagine Institute, Paris Descartes-Sorbonne Paris Cité University, 75015 Paris, France; Institut national de la transfusion sanguine (INTS), 75015 Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Dussiot
authors.affiliation: Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France; Inserm U1163/CNRS ERL 8254, Imagine Institute, Paris Descartes-Sorbonne Paris Cité University, 75015 Paris, France; Institut national de la transfusion sanguine (INTS), 75015 Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Amireault
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane Innovation Park, Don Young Road, Nathan, QLD 4111, Australia.
authors.forename: V M
authors.initials: VM
authors.lastname: Avery
authors.affiliation: CIMI-Paris U1135, équipe 4, hôpital La Pitié-Salpêtrière, 75013 Paris, France; Laboratoire d'excellence GR-Ex, 24, boulevard du Montparnasse, 75015, Paris, France. Electronic address: pabuffet@gmail.com.
authors.forename: P A
authors.initials: PA
authors.lastname: Buffet
mesh_terms: D000962:Antimalarials / Q000494:pharmacology / Q000627:therapeutic use; D032701:Biomimetics; D001793:Blood Preservation / Q000009:adverse effects; D001803:Blood Transfusion; D004353:Drug Evaluation, Preclinical; D004867:Equipment Design; D004905:Erythrocyte Aging; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology; D004913:Erythrocytes, Abnormal; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008288:Malaria / Q000097:blood / Q000188:drug therapy / Q000628:therapy; D008863:Microspheres; D018512:Parasitemia / Q000097:blood / Q000188:drug therapy / Q000628:therapy; D010961:Plasmodium / Q000187:drug effects / Q000254:growth & development; D013154:Spleen / Q000502:physiology*
chemical_list: D000962:Antimalarials
keywords: Applications; Applications thérapeutiques; Deformability; Déformabilité; Erythrocyte; Filtration; Malaria; Microspheres; Microsphères; Paludisme; Rate; Spleen; Transfusion; Érythrocyte