title: Peripheral blood-derived cytokine gene polymorphisms and metabolic profile in women with polycystic ovary syndrome.
abstract: BACKGROUND
  The imbalance between proinflammatory and anti-inflammatory pathways plays a role in polycystic ovary syndrome (PCOS) etiology. We aimed to investigate the relationship between polymorphisms of genes encoding inflammation-associated cytokines and the metabolic profile of Brazilian women with PCOS.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  The study included 196 women - 97 with PCOS (diagnosed based on Rotterdam criteria, 2003) and 99 age-matched, healthy women (controls). It was investigated polymorphisms in cytokines genes from peripheral blood-derived DNA by using PCR.
  
  
  RESULTS
  The frequencies of alleles, genotypes, and phenotypes were similar between women with PCOS and controls. The GG genotype of the -179C/G polymorphism (IL6) was associated with higher glucose levels, while the GA and AA genotypes of the -1082A/G polymorphism (IL10), CT and TT genotypes of the -819A/T polymorphism (IL10), CA and AA genotypes of the -522A/G (IL10) polymorphism, and TA genotype of the +874T/A polymorphism (IFN-γ) were associated with lower total cholesterol and triglycerides levels. The GA genotype of the -1082A/G polymorphism (IL10) and the CC genotype of the 10T/C polymorphism (TGF-β1) were associated with lower and higher Ferriman indices, respectively, in women with PCOS. The AA genotype of the -1082A/G polymorphism (IL10) was associated with lower glucose levels, while the TC genotype of the 10T/C polymorphism (TGF-β1) was associated with a lower lipid accumulation product index and higher high-density lipoprotein cholesterol levels in the PCOS group.
  
  
  CONCLUSIONS
  The genetic polymorphisms of cytokines are not associated with PCOS development, but may contribute to common metabolic disorders associated with PCOS.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Mirelle O
authors.initials: MO
authors.lastname: Sóter
authors.affiliation: Colégio Técnico, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Cláudia N
authors.initials: CN
authors.lastname: Ferreira
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Sales
authors.affiliation: Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Candido
authors.affiliation: Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Reis
authors.affiliation: Simile, Laboratório de Imunologia, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Kátia S
authors.initials: KS
authors.lastname: Milagres
authors.affiliation: Simile, Laboratório de Imunologia, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Ronda
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ieda O
authors.initials: IO
authors.lastname: Silva
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Marinez O
authors.initials: MO
authors.lastname: Sousa
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: karinabgb@gmail.com.
authors.forename: Karina B
authors.initials: KB
authors.lastname: Gomes
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D001938:Brazil; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance; D008075:Lipoproteins, HDL / Q000097:blood; D055442:Metabolome; D008875:Middle Aged; D010641:Phenotype; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D016207:Cytokines; D008075:Lipoproteins, HDL
keywords: Cytokine; Gene; Metabolism; Polycystic ovary syndrome; Polymorphisms