title: Interferon-α curbs production of interleukin-22 by human peripheral blood mononuclear cells exposed to live Borrelia burgdorferi.
abstract: Cytokine networks initiated by means of innate immunity are regarded as a major determinant of host defence in response to acute infection by bacteria including Borrelia burgdorferi. Herein, we demonstrate that interferon (IFN)-α, either endogenously produced after exposure of cells to toll-like receptor-9-activating CpG oligonucleotides or provided as recombinant cytokine, weakens activation of the anti-bacterial interleukin (IL)-1/IL-22 axis in human peripheral blood mononuclear cells exposed to viable B. burgdorferi. As IFN-α has been related to pathological dissemination of the spirochaete, data suggest an immunoregulatory role of type I IFN in this context that is able to significantly modify cytokine profiles thereby possibly determining early course of B. burgdorferi infection.
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, University Hospital Goethe-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Berner
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, University Hospital Goethe-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Malte
authors.initials: M
authors.lastname: Bachmann
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, University Hospital Goethe-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Pfeilschifter
authors.affiliation: Institute of Medical Microbiology and Infection Control, University Hospital Goethe-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kraiczy
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, University Hospital Goethe-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Mühl
mesh_terms: D025065:Borrelia burgdorferi / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007378:Interleukins / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D000097043:Interleukin-22
chemical_list: D016898:Interferon-alpha; D007378:Interleukins; D009838:Oligodeoxyribonucleotides; D000097043:Interleukin-22; C408982:CPG-oligonucleotide
keywords: Borrelia burgdorferi; host defence; immune response; inflammation; interferon-α; interleukin-1; interleukin-22; peripheral blood mononuclear cells