title: Optimization of ultrasonic extraction of polysaccharides from Hovenia dulcis peduncles and their antioxidant potential.
abstract: An ultrasonic-assisted extraction of polysaccharides from the ripe peduncles of Hovenia dulcis (HDPs) was investigated. Response surface methodology along with a Box-Behnken design based on single-factor experiments was employed to optimize and model the extraction conditions of HDPs, namely extraction temperature (40-60 °C), ultrasonic power (320-480 W) and extraction time (35-65 min). The optimized conditions were extraction temperature 60 °C, ultrasonic power 362 W and extraction time 65 min. Under these conditions, the maximal yield of crude HDPs was 25.12±0.145 mg/g DW, which is consistent with the predictive yield of 25.33 mg/g DW. The polysaccharides were graded by an ethanol precipitation method and three fractions (HDPs1, HDPs2 and HDPs3) were harvested. These had final ethanol concentrations of 40%, 60% and 80%, respectively and were acidic polysaccharides. The preliminary characterization was mainly composed of Ara, Rha, Glu and Gal, and exhibited an almost similar characteristic absorption peak by gas chromatograph and infrared spectra analysis. The antioxidant activity assays in vitro revealed that HDPs can be used as natural antioxidants in functional foods and pharmaceutical industries.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: Liuy99999@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Qiang
authors.affiliation: College of Life Science, Zaozhuang University, Zaozhuang 277160, China.
authors.forename: Zhongguan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Du
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification*; D005609:Free Radicals / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D027823:Rhamnaceae / Q000737:chemistry*; D013010:Sonication; D013696:Temperature
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activities; Hovenia dulcis; Polysaccharides; Response surface methodology; Ultrasonic extraction