title: Antibacterial and antioxidant properties of mixed linkage beta-oligosaccharides from extracted β-glucan hydrolysed by Penicillium occitanis EGL lichenase.
abstract: The aim of this study was first to ascertain the chemical composition and the physicochemical properties of cereal extracted β-glucan from barley flour. Secondly, to assess the antioxidant properties and the antibacterial properties of extracted β-glucan hydrolysates. The proximate composition, FT-IR and scanning electron microscopy of extracted β-Glucan were studied. Hydrolysates from extracted β-glucan, obtained by lichenase EGL from Penicillium occitanis, were a mixed linkage beta-oligosaccharides (MLBO) of trisaccharides and tetrasaccharides. MLBO showed a DPPH radical scavenger with IC50 about 1.8 ± 0.01 mg/mL whereas the IC50 of extracted β-glucan was about 5 ± 0.01 mg/mL. MLBO showed a high antioxidative capacity (175 μmol/mL α-tocopherol equivalents) at 5 mg/mL. The antimicrobial activity was confirmed against all tested bacteria especially at 20 mg/mL of MLBO while no inhibition was observed for all the strains used after the addition of either EGL or extracted β-glucan.
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Chaari
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Lilia
authors.initials: L
authors.lastname: Belghith-Fendri
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Zaouri-Ellouzi
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Dorra
authors.initials: D
authors.lastname: Driss
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Monia
authors.initials: M
authors.lastname: Blibech
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kallel
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Bouaziz
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Mehdi
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.|b Unité de service commun bioréacteur couplé à un ultrafiltre , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouz-Chaabouni
authors.affiliation: a Unité Enzymes et Bioconversion, Biology Department , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.|b Unité de service commun bioréacteur couplé à un ultrafiltre , Sfax National School of Engineers, Sfax University , B.P 1173 - 3038 Sfax Cedex , Tunisia.
authors.forename: Raoudha
authors.initials: R
authors.lastname: Ghorbel
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D005433:Flour; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D001467:Hordeum / Q000737:chemistry; D006868:Hydrolysis; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology*; D010407:Penicillium / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D024502:alpha-Tocopherol / Q000494:pharmacology; D047071:beta-Glucans / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D009844:Oligosaccharides; D047071:beta-Glucans; D006026:Glycoside Hydrolases; C028020:licheninase; D024502:alpha-Tocopherol
keywords: DPPH radical-scavenging; Extracted β-glucan; antibacterial activity; total antioxidant capacity; β-glucan hydrolysates