title: Antitumor activity of tatariside F isolated from roots of Fagopyrum tataricum (L.) Gaertn against H22 hepatocellular carcinoma via up-regulation of p53.
abstract: BACKGROUND
  Fagopyrum tataricum (L.) Gaertn is a famous drinking food and herbal medicine in China, and have been commonly used for treating various diseases.
  
  
  OBJECTIVE
  This study was designed to investigate the antitumor effect of tatariside F (TF) isolated from the roots of F. tataricum against H22 hepatocellular carcinoma (HCC) in vitro and in vivo and explore the possible mechanisms.
  
  
  METHODS
  In our present study, the anti-proliferative effect of TF against H22 cells was evaluated by MTT method. Furthermore, a mice xenograft model was established to investigate the antitumor effect of TF on HCC in vivo, and the possible mechanisms were determined by western blot and fluorescence polarization binding assay. In addition, the protective effect of TF on liver was also investigated by examining the histopathological changes and determining the liver biochemical parameters.
  
  
  RESULTS
  Our results demonstrated that TF possessed notable antitumor effect against HCC both in vivo and in vitro, and the possible mechanism might be related to up-regulation of the protein expressions of Bax and p53, and down-regulation of Bcl-2. What's more, TF also exhibited protective effects against CTX (cyclophosphamide)-induced liver damages when co-administrated with CTX.
  
  
  CONCLUSIONS
  Our study suggested that TF possess notable antitumor effects against HCC and might play a favorable role in drug combination therapy against tumors with protective effect on liver.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China; Department of Natural Products Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, P.R. China.
authors.forename: Changling
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Shu
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China. Electronic address: qinsmmu@126.com.
authors.forename: Luping
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, P.R. China. Electronic address: zhengchengjian@smmu.edu.cn.
authors.forename: Chengjian
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000187:drug effects; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; C000595707:BCL2 protein, human; D005419:Flavonoids; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C000601574:tatariside F
keywords: Apoptosis; Fagopyrum tataricum (L.) Gaertn; H22 cell line; Hepatocellular carcinoma; Tatariside F; p53