title: Heijiangdan ointment relieves oxidative stress from radiation dermatitis induced by (60)Co γ-ray in mice.
abstract: OBJECTIVE
  To investigate the effects of Heijiangdan Ointment ( HJD) on oxidative stress in (60)Co γ-ray radiation-induced dermatitis in mice.
  
  
  METHODS
  Female Wistar mice with grade 4 radiation dermatitis induced by (60)Co γ-rays were randomly divided into four groups (n=12 per group); the HJD-treated, recombinant human epidermal growth factor (rhEGF)-treated, Trolox-treated, and untreated groups, along with a negative control group. On the 11th and 21st days after treatment, 6 mice in each group were chosen for evaluation. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), and lactate dehydrogenase (LDH) were detected using spectrophotometric methods. The fibroblast mitochondria were observed by transmission electron microscopy (TEM). The expressions of fibroblast growth factor 2 (FGF-2) and transforming growth factor β1 (TGF-β1) were analyzed by western blot.
  
  
  RESULTS
  Compared with the untreated group, the levels of SOD, MDA and LDH, on the 11th and 21st days after treatment showed significant difference (P<0.05). TEM analysis indicated that fibroblast mitochondria in the untreated group exhibited swelling and the cristae appeared fractured, while in the HJD group, the swelling of mitochondria was limited and the rough endoplasmic reticulum appeared more relaxed. The expressions of FGF-2 and TGF-β1 increased in the untreated group compared with the negative control group (P<0.05). After treatment, the expression of FGF-2, rhEGF and Trolox in the HJD group were significantly increased compared with the untreated group (P<0.05), or compared with the negative control group (P<0.05). The expression of TGF-β1 showed significant difference between untreated and negative control groups (P<0.05). HJD and Trolox increased the level of TGF-β1 and the difference was marked as compared with the untreated and negative control groups (P<0.05).
  
  
  CONCLUSIONS
  HJD relieves oxidative stress-induced injury, increases the antioxidant activity, mitigates the fibroblast mitochondrial damage, up-regulates the expression of growth factor, and promotes mitochondrial repair in mice.
authors.affiliation: Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, 100010, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, 100010, China.
authors.forename: Ming-wei
authors.initials: MW
authors.lastname: Yu
authors.affiliation: Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, 100010, China. ntxm100@sina.com.
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, 100010, China.
authors.forename: Guo-wang
authors.initials: GW
authors.lastname: Yang
authors.affiliation: Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, 100010, China.
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Luo
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, 100039, China.
authors.forename: Rui-yun
authors.initials: RY
authors.lastname: Peng
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, 100039, China.
authors.forename: Ya-bing
authors.initials: YB
authors.lastname: Gao
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, 100039, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, 100039, China.
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000818:Animals; D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D003872:Dermatitis / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D009824:Ointments; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D004364:Pharmaceutical Preparations; D011832:Radiation Injuries / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000528:radiation effects
chemical_list: D001688:Biological Products; D003037:Cobalt Radioisotopes; D004365:Drugs, Chinese Herbal; D009824:Ointments; D004364:Pharmaceutical Preparations; D053773:Transforming Growth Factor beta1; C000612393:xueyu danhuang; D016222:Fibroblast Growth Factor 2; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase
keywords: Chinese medicine; Heijiangdan Ointment; antioxidant; fibroblast mitochondria; growth factor; oxidative stress; radiation dermatitis; transmission electron microscope