title: The Role of the Two-Component System BaeSR in Disposing Chemicals through Regulating Transporter Systems in Acinetobacter baumannii.
abstract: Bacterial two-component regulatory systems (TCSs) facilitate changes in gene expression in response to environmental stimuli. TCS BaeR regulons influence tigecycline susceptibility in Acinetobacter baumannii through positively regulating the pump genes adeA and adeB. In this study, we demonstrate that an additional two transport systems, AdeIJK and MacAB-TolC, are also regulated by BaeSR. In the wild type and clinical tigecycline-resistant A. baumannii strains, gene expression of AdeIJK and MacAB-TolC increased after tigecycline induction, implicating their importance to tigecycline resistance in addition to AdeABC. Phenotypic microarray results showed that A. baumannii is vulnerable to certain chemicals, especially tannic acid, after deleting baeR, which was confirmed using the spot assay. The wild-type strain of A. baumannii also exhibited 1.6-fold and 4.4-fold increase in gene expression of adeJ and macB in the medium with 100 μg/mL tannic acid, but the increase was fully inhibited by baeR deletion. An electrophoretic motility shift assay based on an interaction between His-BaeR and the adeA, adeI and macA promoter regions did not demonstrate direct binding. In conclusion, A. baumannii can use the TCS BaeSR in disposing chemicals, such as tannic acid and tigecycline, through regulating the efflux pumps.
authors.affiliation: Department of Medicine, National Taiwan University Hospital Chu-Tung Branch, Hsin-Chu City, Taiwan; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsin-Chu City, Taiwan.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Lin
authors.affiliation: Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsin-Chu City, Taiwan.
authors.forename: Yun-You
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsin-Chu City, Taiwan; Department of Life Science, National Tsing Hua University, Hsin-Chu City, Taiwan.
authors.forename: Chung-Yu
authors.initials: CY
authors.lastname: Lan
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives* / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D013634:Tannins / Q000494:pharmacology; D000078304:Tigecycline
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D013634:Tannins; D000078304:Tigecycline; D008911:Minocycline