title: Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway.
abstract: Girdin, an actin‑binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound‑healing assay, transwell invasion assay, reverse transcription‑quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol‑3‑kinase/protein kinase B (PI3K‑Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.
authors.affiliation: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Ni
authors.affiliation: Department of Human Anatomy, Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Zhaoxue
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Fuxin
authors.initials: F
authors.lastname: Yi
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Tong
mesh_terms: D017209:Apoptosis; D001681:Biological Assay; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015194:Diffusion Chambers, Culture; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D033921:Vesicular Transport Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: C502451:CCDC88A protein, human; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9