title: Developmental inhibition and DNA damage of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) by gamma radiation.
abstract: OBJECTIVE
  Gamma radiation on Helicoverpa armigera Hübner was performed to assess developmental inhibition and to identify a potential quarantine treatment dose of the radiation.
  
  
  METHODS
  Gamma radiation ((60)Co) treatment at different doses of 50, 100, 200, 300, and 400 Gy was carried out with egg, larvae, pupae and adults of H. armigera.
  
  
  RESULTS
  Gamma radiation induced developmental inhibition of all stages of H. armigera. The effective dose values required for inhibition 99% (ED(99)) of hatching, pupation and adult emergence from the irradiated eggs were 550.7, 324.9 and 136.4 Gy, respectively. ED(99) values for inhibition of the larvae to adult emergence was 200.0 Gy. Irradiation on pupae could not completely inhibit adult emergence even at 400 Gy. ED(99) value for inhibition of F(1) egg hatchability from the irradiated adults was estimated to be 229.5 Gy.
  
  
  CONCLUSIONS
  This study suggests that gamma radiation is a possible alternative to phytosanitary treatments. Irradiation treatment with minimum dose of 200 Gy can be suggested as optimum dose for larval treatment in quarantine.
authors.affiliation: a Institute of Agriculture and Life Science (BK21+program), Gyeongsang National University , Jinju, Gyeongnam , Republic of Korea.
authors.forename: Junheon
authors.initials: J
authors.lastname: Kim
authors.affiliation: b Division of Applied Life Science (BK21+program), Gyeongsang National University , Jinju, Gyeongnam , Republic of Korea.
authors.forename: Soon-Oh
authors.initials: SO
authors.lastname: Chung
authors.affiliation: b Division of Applied Life Science (BK21+program), Gyeongsang National University , Jinju, Gyeongnam , Republic of Korea.
authors.forename: Miyeon
authors.initials: M
authors.lastname: Jang
authors.affiliation: c Institute of Life Science, Gyeongsang National University , Jinju, Gyeongnam , Republic of Korea.
authors.forename: Sin Ae
authors.initials: SA
authors.lastname: Jang
authors.affiliation: a Institute of Agriculture and Life Science (BK21+program), Gyeongsang National University , Jinju, Gyeongnam , Republic of Korea.|b Division of Applied Life Science (BK21+program), Gyeongsang National University , Jinju, Gyeongnam , Republic of Korea.
authors.forename: Chung Gyoo
authors.initials: CG
authors.lastname: Park
mesh_terms: D000818:Animals; D001947:Breeding; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D005720:Gamma Rays; D007814:Larva / Q000254:growth & development / Q000528:radiation effects; D008297:Male; D009036:Moths / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects*; D010571:Pest Control; D011679:Pupa / Q000254:growth & development / Q000528:radiation effects
keywords: Gamma radiation; comet assay; cotton bollworm; developmental inhibition; phytosanitary; quarantine