title: Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders.
abstract: Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.
authors.affiliation: Centre Hospitalier Universitaire Vaudois (CHUV) , Centre de Neurosciences Psychiatriques, Département de Psychiatrie, Site de Cery, Prilly/Lausanne CH-1008, Switzerland ; Brain Mind Institute , École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Marquet
authors.affiliation: Brain Mind Institute , École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland ; King Abdullah University of Science and Technology (KAUST) , Division of Biological and Environmental Sciences and Engineering, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Christian
authors.initials: C
authors.lastname: Depeursinge
authors.affiliation: Centre Hospitalier Universitaire Vaudois (CHUV) , Centre de Neurosciences Psychiatriques, Département de Psychiatrie, Site de Cery, Prilly/Lausanne CH-1008, Switzerland ; Brain Mind Institute , École Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland ; King Abdullah University of Science and Technology (KAUST) , Division of Biological and Environmental Sciences and Engineering, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Magistretti
keywords: absolute cell volume; cell imaging; co-transporters; dendritic spines; diffraction tomography; digital holographic microscopy; early cell biomarkers; glutamate; high-throughput screening; neuronal activities; psychiatry disorders; quantitative phase microscopy; transmembrane water movements