title: Nanostructured gold dsDNA sensor for early detection of breast cancer by beta protein 1 (BP1).
abstract: Beta protein 1 (BP1) is a homeobox protein expressed in 80% of breast cancer cells in either estrogen receptor (ER) positive or ER negative breast cancer. However, it is barely detectable in normal breast tissues. In this project we present an electrochemical DNA nanostructured gold biosensor for detection of BP1. The gold sensor is first electrochemically nanostructured in 0.5 M sulfuric acid to reach superior conductivity, larger surface area, and higher stability. Nanostructured gold surface was characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The nanostructured gold sensor is then modified with double-stranded (ds) DNA mapping the genomic sequence that contains the binding site for BP1. A redox-active probe (methylene blue) was intercalated in dsDNA to monitor the binding event of BP1. A linear correlation of the electrochemical response by concentration of BP1was obtained (R2= 0.998) with a limit of detection of 1.2 nM. This nanostructured gold dsDNA sensor is shown to be sensitive, selective, stable, and reusable allowing for its potential clinical use.
authors.affiliation: Department of Chemistry, University of Puerto Rico, PO Box 9019 Mayagüez, PR 00681.
authors.forename: Wanda I
authors.initials: WI
authors.lastname: Pérez
authors.affiliation: Industrial Biotechnology Program, University of Puerto Rico, PO Box 9019 Mayagüez, PR 00681.
authors.forename: Yarelys
authors.initials: Y
authors.lastname: Soto
authors.affiliation: Engineering Science and Materials Department, University of Puerto Rico, PO Box 9019 Mayagüez, PR 00681.
authors.forename: Jaime E
authors.initials: JE
authors.lastname: Ramirez-Vick
authors.affiliation: Department of Chemistry, University of Puerto Rico, PO Box 9019 Mayagüez, PR 00681.
authors.forename: Enrique
authors.initials: E
authors.lastname: Meléndez
keywords: DNA sensor; Gold nanostructed sensor; beta protein 1; biosensor