title: (1)H, (13)C, and (15)N backbone resonance assignments of the full-length 40 kDa S. acidocaldarius Y-family DNA polymerase, dinB homolog.
abstract: The dinB homolog (Dbh) is a member of the Y-family of translesion DNA polymerases, which are specialized to accurately replicate DNA across from a wide variety of lesions in living cells. Lesioned bases block the progression of high-fidelity polymerases and cause detrimental replication fork stalling; Y-family polymerases can bypass these lesions. The active site of the translesion synthesis polymerase is more open than that of a replicative polymerase; consequently Dbh polymerizes with low fidelity. Bypass polymerases also have low processivity. Short extension past the lesion allows the high-fidelity polymerase to switch back onto the site of replication. Dbh and the other Y-family polymerases have been used as structural models to investigate the mechanisms of DNA polymerization and lesion bypass. Many high-resolution crystal structures of Y-family polymerases have been reported. NMR dynamics studies can complement these structures by providing a measure of protein motions. Here we report the (15)N, (1)H, and (13)C backbone resonance assignments at two temperatures (35 and 50 °C) for Sulfolobus acidocaldarius Dbh polymerase. Backbone resonance assignments have been obtained for 86 % of the residues. The polymerase active site is assigned as well as the majority of residues in each of the four domains.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
authors.forename: Sean L
authors.initials: SL
authors.lastname: Moro
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA. mcocco@uci.edu.|Department of Pharmaceutical Sciences, University of California, 1218 Natural Sciences I, Irvine, CA, 92697-3900, USA. mcocco@uci.edu.
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Cocco
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009587:Nitrogen Isotopes; D066244:Proton Magnetic Resonance Spectroscopy; D017386:Sequence Homology, Amino Acid; D016944:Sulfolobus / Q000201:enzymology*
chemical_list: D019843:Archaeal Proteins; D009587:Nitrogen Isotopes; D004259:DNA-Directed DNA Polymerase
keywords: Bypass polymerase; Dbh (dinB homolog); Sulfolobus acidocaldarius; Translesion synthesis; Y-family polymerase