title: Effect of method of caries induction on aged resin-dentin bond of primary teeth.
abstract: BACKGROUND
  To investigate the influence of chemical and microbiological methods of caries induction on bond degradation of adhesive systems to primary dentin.
  
  
  METHODS
  Flat dentin surfaces from 36 primary molars were assigned to three groups (n = 12) according to method to induce caries-affected dentin: (1) control (sound dentin); (2) pH-cycling; and (3) microbiological caries induction model. Teeth were submitted to caries induction for 14 days for both methods, and the sound dentin was stored in distilled water during the same period. Specimens from each experimental group were then randomly reassigned to two subgroups (n = 6) according to the adhesive system tested: two-step etch-and-rinse adhesive (Adper Single Bond 2 - SB) or two-step self-etch system (Clearfil SE Bond - CSEB). Composite buildups were constructed and sectioned to obtain bonded sticks to be subjected to microtensile (μTBS) testing immediately or after 12 months of water aging. The μTBS means were analyzed by three-way repeated measures ANOVA and Tukey's tests (α = 0.05).
  
  
  RESULTS
  The μTBS values obtained to artificially-created caries-affected dentin were lower compared with sound dentin, but were not affected by method of caries induction. Water storage for 12 months reduced bond strengths, except to CSEB bonded to sound dentin.
  
  
  CONCLUSIONS
  Chemical and microbiological methods affect similarly the stability of resin-dentin bonds in primary teeth.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, 05508-000, Brazil. tathilenzi@usp.br.
authors.forename: Tathiane Larissa
authors.initials: TL
authors.lastname: Lenzi
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, 05508-000, Brazil. anacalvo@usp.br.
authors.forename: Ana Flávia Bissoto
authors.initials: AF
authors.lastname: Calvo
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, 05508-000, Brazil. tamarakt@usp.br.
authors.forename: Tamara Kerber
authors.initials: TK
authors.lastname: Tedesco
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, Universidade Estadual Paulista (Unesp), Rua Humaitá 1680, 14801-903, Araraquara, SP, Brazil. hericaricci@yahoo.com.br.
authors.forename: Hérica Adad
authors.initials: HA
authors.lastname: Ricci
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, Universidade Estadual Paulista (Unesp), Rua Humaitá 1680, 14801-903, Araraquara, SP, Brazil. jhebling@foar.unesp.br.
authors.forename: Josimeri
authors.initials: J
authors.lastname: Hebling
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, 05508-000, Brazil. danielar@usp.br.
authors.forename: Daniela Prócida
authors.initials: DP
authors.lastname: Raggio
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D018441:Biofilms; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003731:Dental Caries / Q000209:etiology* / Q000382:microbiology; D003738:Dental Cements / Q000737:chemistry; D003764:Dental Materials / Q000737:chemistry; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000382:microbiology / Q000648:ultrastructure*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055115:Light-Curing of Dental Adhesives / Q000379:methods; D008422:Materials Testing; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry*; D013295:Streptococcus mutans / Q000502:physiology; D013314:Stress, Mechanical; D013718:Tensile Strength; D013997:Time Factors; D014094:Tooth, Deciduous / Q000382:microbiology / Q000648:ultrastructure*; D014867:Water / Q000737:chemistry
chemical_list: C554641:Adper single bond 2; C438313:Clearfil SE Bond; D003188:Composite Resins; D003738:Dental Cements; D003764:Dental Materials; C444701:Filtek Z250; D019279:Resin Cements; D014867:Water