title: Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four "Kidney-Tonifying" Traditional Chinese Herbs.
abstract: Traditional Chinese medicine can promote the proliferation of bone marrow-derived mesenchymal stem cells (BMSCs). We chose four "Kidney-tonifying" Chinese herbal medicines, Radix Astragali, Salvia, Herba Epimedii, and Saussurea Involucrata, to evaluate whether they had positive effects on the proliferation of BMSCs and TGF-β1-induced chondrogenic differentiation of BMSCs. The four Chinese herbal medicines were intragastrically administered to Sprague-Dawley rats, respectively, to prepare drug-containing serums of corresponding Chinese herbs. BMSCs were isolated, cultured, and exposed to culture solution containing 1%, 5%, 10%, and 15% (v/v) Radix Astragali-, Salvia-, Herba Epimedii-, and Saussurea Involucrata-containing serum, respectively. TGF-β1-induced BMSCs were addressed in the same manner. Collagen type II protein was assessed by immunofluorescence methods. To assess whether the drug-containing serums had positive effects on the proliferation of BMSCs and TGF-β1-induced BMSCs, MTT method was assessed. The proliferation of BMSCs was significantly enhanced when exposed to culture solutions containing 1% and 5% Radix Astragali-, 1% and 5% Salvia-, 5% Herba Epimedii-, and 1%, 5%, and 10% Saussurea Involucrata-containing serum. The proliferation of TGF-β1-induced BMSCs was significantly enhanced when exposed to 1%, 5%, and 15% Radix Astragali-, 10% and 15% Salvia-, 5%, and 15% Herba Epimedii-, and 1%, 5%, and 10% Saussurea Involucrata-containing serum.
authors.affiliation: Department of Colorectal Surgery, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cai
authors.affiliation: Department of Pediatric Surgery, Wuxi People's Hospital, Wuxi, Jiangsu 214000, China.
authors.forename: Ai-guo
authors.initials: AG
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Wuxi Hospital of Traditional Chinese Medicine, Wuxi, Jiangsu 214000, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Wuxi Hospital of Traditional Chinese Medicine, Wuxi, Jiangsu 214000, China.
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Ge
authors.affiliation: Department of Orthopedics, Wuxi Hospital of Traditional Chinese Medicine, Wuxi, Jiangsu 214000, China.
authors.forename: Guo-da
authors.initials: GD
authors.lastname: Dai
authors.affiliation: Life School, Nantong University, Nantong, Jiangsu 226000, China.
authors.forename: Xiang-ling
authors.initials: XL
authors.lastname: Tan
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Guangxi Medical University, Nanningm, Guangxi 530021, China.
authors.forename: Gopaul
authors.initials: G
authors.lastname: Roodrajeetsing
authors.affiliation: Department of Orthopedics, Wuxi Hospital of Traditional Chinese Medicine, Wuxi, Jiangsu 214000, China.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Cai
mesh_terms: D000818:Animals; D027885:Astragalus propinquus; D001854:Bone Marrow Cells / Q000166:cytology; D001892:Camphanes; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D053723:Panax notoginseng; D051381:Rats; D027561:Salvia miltiorrhiza
chemical_list: D001892:Camphanes; D004365:Drugs, Chinese Herbal; C000604051:danshen dripping pill; C027492:Huang Qi