title: Carposina sasakii (Lepidoptera: Carposinidae) in its Native Range Consists of Two Sympatric Cryptic Lineages as Revealed by Mitochondrial COI Gene Sequences.
abstract: The genetic differentiation and genetic structure of the peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), was investigated in China, where the moth is native. The mitochondrial cytochrome c oxidase I (COI) gene of 180 individuals from 16 collections were sequenced and analyzed. The results showed that two sympatric and cryptic mtDNA lineages existed within C. sasakii in China. The genetic differentiation has significant correlation with the geographical distance, but has no evidence for host plant associations. Our results of haplotype distribution suggest that the C. sasakii individuals can naturally move between areas, while the movement of individuals between long-distance locations may be associated with human activities such as the transport of fruit. Finally, an mitochondrial COI gene PCR-RFLP method was developed to differentiate the two cryptic mtDNA lineages within C. sasakii, which provides rapid and reliable tool for the future research of the two lineages.
authors.affiliation: Key Laboratory for Plant Virology of Shandong Province, Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory for Plant Virology of Shandong Province, Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory for Plant Virology of Shandong Province, Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China chudong1977@hotmail.com zbsli3@163.com.
authors.forename: L-L
authors.initials: LL
authors.lastname: Li
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: D
authors.initials: D
authors.lastname: Guo
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Tao
authors.affiliation: Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China chudong1977@hotmail.com zbsli3@163.com.
authors.forename: D
authors.initials: D
authors.lastname: Chu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D003576:Electron Transport Complex IV / Q000235:genetics; D049810:Genetic Speciation; D019476:Insect Proteins / Q000235:genetics; D007814:Larva / Q000145:classification / Q000254:growth & development; D024101:Mitochondrial Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D016415:Sequence Alignment; D061350:Sympatry
chemical_list: D019476:Insect Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV
keywords: Carposina sasakii Matsumura; cryptic mtDNA lineages; genetic differentiation; mitochondrial COI gene