title: Caldariomyces fumago DSM1256 Contains Two Chloroperoxidase Genes, Both Encoding Secreted and Active Enzymes.
abstract: Inspection of transcriptome data from the chloroperoxidase (CPO)-producing fungus Caldariomyces fumago DSM1256 led to the discovery of two distinct CPO mRNA sequences. This strain could be shown to contain the newly identified isogene as well as produce and secrete both isoenzymes. The CPO2 enzyme bears high sequence similarity to the well-characterized CPO (87% identity for the mature proteins). It shows two insertions in the signal peptide and in the C-terminal propeptide, and one deletion in the mature polypeptide close to the C-terminus. Furthermore, it lacks one of the serine residues known to be O-glycosylated in the CPO sequence. The demonstration of a CPO isogene which is expressed as a secreted and active CPO clarifies the nature of this isoenzyme already identified in earlier reports. A structure model comparison shows a high conservation of the active site and the substrate channel, suggesting very similar catalytic properties.
authors.affiliation: DECHEMA Research Institute, Frankfurt am Main, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Buchhaupt
authors.forename: Sonja
authors.initials: S
authors.lastname: Hüttmann
authors.forename: Christian Carsten
authors.initials: CC
authors.lastname: Sachs
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bormann
authors.forename: Achim
authors.initials: A
authors.lastname: Hannappel
authors.forename: Jens
authors.initials: J
authors.lastname: Schrader
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D002711:Chloride Peroxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins; D007527:Isoenzymes; D002711:Chloride Peroxidase