title: Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes in rat primary white adipocytes.
abstract: It is widely accepted that lipolysis in adipocytes are regulated through the enzymatic activation of both hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) via their phosphorylation events. Accumulated evidence shows that habitual exercise training (HE) enhances the lipolytic response in primary white adipocytes with changes in the subcellular localization of lipolytic molecules. However, no study has focused on the effect that HE exerts on the phosphorylation of both HSL and ATGL in primary white adipocytes. It has been shown that the translocation of HSL from the cytosol to lipid droplet surfaces requires its phosphorylation at Ser-563. In primary white adipocytes obtained from HE rats, the level of HSL and ATGL proteins was higher than that in primary white adipocytes obtained from sedentary control (SC) rats. In HE rats, the level of phosphorylated ATGL and HSL was also significantly elevated compared with that in SC rats. These differences were confirmed by Phos-tag SDS-PAGE, a technique used to measure the amount of total phosphorylated proteins. Our results suggest that HE can consistently increase the activity of both lipases, thereby enhancing the lipolysis in white fat cells. Thus, HE helps in the prevention and treatment of obesity-related diseases by enhancing the lipolytic capacity.
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan. Electronic address: junetsu@ks.kyorin-u.ac.jp.
authors.forename: Junetsu
authors.initials: J
authors.lastname: Ogasawara
authors.affiliation: Graduate School of Health and Sports Sciences, Doshisha University, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Izawa
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Shirato
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Ishibashi
authors.affiliation: Graduate School of Health and Sports Sciences, Doshisha University, Kyotanabe, Kyoto 610-0394, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ohira
authors.affiliation: Department of Third Internal Medicine, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishida
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kizaki
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism; D008066:Lipolysis / Q000235:genetics; D008297:Male; D009765:Obesity / Q000517:prevention & control*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D061251:Primary Cell Culture; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism*; D000217:Acyltransferases
chemical_list: D008049:Lipase; D002787:Sterol Esterase; C571275:PNPLA2 protein, rat; D000217:Acyltransferases
keywords: Adipose triglyceride lipase; Hormone-sensitive lipase; Phosphorylation; Primary white adipocytes; Rat