title: Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway.
abstract: Investigations into the role of hypoxia have concentrated on hypoxic damage to cells and the associated adaptative mechanisms, however, the effects of hypoxia on cell proliferation and differentiation have received less attention. The present study aimed to investigate the role of hypoxia on the proliferation of MC3T3‑E1 cells and examine the molecular mechanism involved. Cells treated with low levels of hypoxia had an increased percentage of S phase cells and a decreased percentage of G1 phase cells, promoted the level of cell proliferation‑associated proteins, proliferating cell nuclear antigen and cyclin D. In addition, hypoxia increased cell proliferation by upregulating the expression of hypoxia‑inducible factor (HIF)‑1α. The phosphoinositide 3‑kinase/Akt and mitogen‑activated protein kinase/extracellular signal‑regulated kinase pathways augmented the expression of HIF‑1α in the MC3T3‑E1 cells. The present study demonstrated that hypoxia induces positive effects on osteoblast proliferation, suggesting a novel strategy in the treatment of osteoporosis.
authors.affiliation: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Buling
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Tian
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015870:Gene Expression; D000860:Hypoxia / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases