title: [Urinary protein detection by iTRAQ® associated with renal transplant complications and its modification with therapy].
abstract: BACKGROUND
  After renal transplant, surgical, infection complications, as well as graft rejection may occur; early detection through non-invasive markers is the key to change therapy and avoid biopsy.
  
  
  OBJECTIVE
  The aime of the study is to determine urine protein profiles in patients undergoing renal transplant with complications and detect its variation when therapy is modified.
  
  
  METHODS
  Urine samples were collected from patients prior the transplant and various postoperative stages. Urinary protein profiles were obtained by peptide labeling using isobaric isotopes for relative quantification (iTRAQ(®)).
  
  
  RESULTS
  A total of 22 patients were included, of whom 12 developed post-transplant complication: 2 with graft rejection (one male and one female) and 10 (6 males and 4 females) in the group of post-transplant infections. Using iTRAQ(®) 15/345 and 28/113 proteins were identified and fulfilled the acceptance criteria, in graft rejection and post-transplant infections group, respectively.
  
  
  CONCLUSIONS
  Albumin was the only protein found in both groups, the remaining proteins were different. The 5 proteins with higher scores in graft rejection were: alpha-1-microglobulin, 5'-nucleotidase cytosolic III, retinol-binding protein 4, membrane protein palmitoylated 4, and serine carboxypeptidase, while post-transplant infections were: mitochondrial acetyl-coenzyme A synthetase, putative adenosyl homocysteinase 2, zinc finger protein GLIS1, putative protein FAM157B, and zinc finger protein 615. It remains to elucidate the involvement of each of these in patients with renal transplantation.
authors.affiliation: Servicio de Trasplantes, Departamento de Cirugía, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Miguel Mariano
authors.initials: MM
authors.lastname: Escobedo-Villarreal
authors.affiliation: Unidad de Hígado, Departamento de Medicina Interna, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Amanda Berenice
authors.initials: AB
authors.lastname: Mercado-Moreira
authors.affiliation: Unidad de Hígado, Departamento de Medicina Interna, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Linda Elsa
authors.initials: LE
authors.lastname: Muñoz-Espinosa
authors.affiliation: Unidad de Hígado, Departamento de Medicina Interna, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Mariana
authors.initials: M
authors.lastname: Gamboa-Esparza
authors.affiliation: Servicio de Trasplantes, Departamento de Cirugía, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
authors.forename: Edelmiro
authors.initials: E
authors.lastname: Pérez-Rodríguez
authors.affiliation: Unidad de Hígado, Departamento de Medicina Interna, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México. Electronic address: paucordero@yahoo.com.mx.
authors.forename: Paula
authors.initials: P
authors.lastname: Cordero-Pérez
mesh_terms: D000328:Adult; D000419:Albuminuria / Q000209:etiology / Q000652:urine; D015415:Biomarkers / Q000652:urine; D005260:Female; D006084:Graft Rejection / Q000652:urine; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007554:Isotopes / Q000652:urine; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011183:Postoperative Complications / Q000209:etiology / Q000652:urine*; D011446:Prospective Studies; D011507:Proteinuria / Q000209:etiology / Q000652:urine*; D011933:Reagent Kits, Diagnostic; D053719:Tandem Mass Spectrometry / Q000379:methods*; D016482:Urinalysis / Q000379:methods*; D014552:Urinary Tract Infections / Q000209:etiology / Q000652:urine*
chemical_list: D015415:Biomarkers; D007554:Isotopes; D010455:Peptides; D011933:Reagent Kits, Diagnostic
keywords: Complicaciones postrasplante renal; Infecciones; Infections; Proteínas urinarias; Rechazo; Rejection; Renal post-transplant complications; Renal transplant; Trasplante renal; Urinary proteins; iTRAQ(®)