title: l-Histidyl-glycyl-glycyl-l-histidine. Amino-acid structuring of the bleomycin-type pentadentate metal-binding environment capable of efficient double-strand cleavage of plasmid DNA.
abstract: A tetrapeptide, l-histidyl-glycyl-glycyl-l-histidine (HGGH), was synthesized and the pUC19 plasmid DNA cleaving activity by copper(II) complex of HGGH (Cu(II)-HGGH) was investigated. Cu(II)-HGGH showed bleomycin-like DNA cleaving activity and, at 50nM, converted a supercoiled DNA efficiently to a linear DNA in the presence of 500μM H2O2/sodium ascorbate through an oxidative pathway.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Ida
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Iwamaru
authors.affiliation: School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Fujita
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kudo
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Kurosaki
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: motsuka@gpo.kumamoto-u.ac.jp.
authors.forename: Masami
authors.initials: M
authors.lastname: Otsuka
mesh_terms: D001205:Ascorbic Acid; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D053837:DNA Cleavage; D004278:DNA, Superhelical; D006861:Hydrogen Peroxide; D009690:Nucleic Acid Conformation; D009842:Oligopeptides / Q000138:chemical synthesis*; D010084:Oxidation-Reduction; D010957:Plasmids
chemical_list: D056831:Coordination Complexes; D004278:DNA, Superhelical; D009842:Oligopeptides; C000607261:histidyl-glycyl-glycyl-l-histidine; D003300:Copper; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: Cleavage; Copper; DNA; Tetrapeptide