title: In vitro efficacy of liposomal amphotericin B, micafungin and fluconazole against non-albicans Candida species biofilms.
abstract: Non-albicans Candida species are being isolated with increasing frequency. In this study, biofilm formation by Candida tropicalis, Candida parapsilosis and Candida glabrata was evaluated and the activities of liposomal amphotericin B (LAB), micafungin (MFG) and fluconazole (FLC) against these biofilms were assessed using a clinically relevant in vitro model system. LAB exhibited strong activities against the three non-albicans Candida species and showed dose-dependent efficacy. MFG displayed a paradoxical growth effect against the C. tropicalis biofilm. FLC was ineffective for non-albicans biofilms. This study shows that Candida biofilms have unique susceptibility to LAB. The dose-dependent effects of LAB indicate that this drug may be a useful treatment for biofilm formation by non-albicans Candida species in cases in which the catheter cannot be removed for clinical reasons.
authors.affiliation: Department of Clinical Infectious Diseases, Aichi Medical University Graduate School of Medicine, Aichi, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kawai
authors.affiliation: Department of Clinical Infectious Diseases, Aichi Medical University Graduate School of Medicine, Aichi, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yamagishi
authors.affiliation: Department of Clinical Infectious Diseases, Aichi Medical University Graduate School of Medicine, Aichi, Japan. Electronic address: mikamo@aichi-med-u.ac.jp.
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Mikamo
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D041221:Candida glabrata / Q000187:drug effects / Q000502:physiology; D041022:Candida tropicalis / Q000187:drug effects / Q000502:physiology; D054714:Echinocandins / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology*; D066298:In Vitro Techniques; D055666:Lipopeptides / Q000494:pharmacology*; D000077551:Micafungin; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; C068538:liposomal amphotericin B; D000666:Amphotericin B; D015725:Fluconazole; D000077551:Micafungin
keywords: Antifungals; Biofilms; Candida glabrata; Candida parapsilosis; Candida tropicalis; Paradoxical growth effect (PGE)