title: Diversity index of mucosal resident T lymphocyte repertoire predicts clinical prognosis in gastric cancer.
abstract: A characteristic immunopathology of human cancers is the induction of tumor antigen-specific T lymphocyte responses within solid tumor tissues. Current strategies for immune monitoring focus on the quantification of the density and differentiation status of tumor-infiltrating T lymphocytes; however, properties of the TCR repertoire ‒ including antigen specificity, clonality, as well as its prognostic significance ‒ remain elusive. In this study, we enrolled 28 gastric cancer patients and collected tumor tissues, adjacent normal mucosal tissues, and peripheral blood samples to study the landscape and compartmentalization of these patients' TCR β repertoire by deep sequencing analyses. Our results illustrated antigen-driven expansion within the tumor compartment and the contracted size of shared clonotypes in mucosa and peripheral blood. Most importantly, the diversity of mucosal T lymphocytes could independently predict prognosis, which strongly underscores critical roles of resident mucosal T-cells in executing post-surgery immunosurveillance against tumor relapse.
authors.affiliation: Department of General Surgery and Center of Minimal Invasive; Southwest Hospital; Third Military Medical University ; Chongqing, China ; Biomedical Analysis Center; Third Military Medical University ; Chongqing, China ; Chongqing Key Laboratory of Cellomics ; Chongqing, China.
authors.forename: Qingzhu
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Department of General Surgery and Center of Minimal Invasive; Southwest Hospital; Third Military Medical University ; Chongqing, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Biomedical Analysis Center; Third Military Medical University ; Chongqing, China ; Chongqing Key Laboratory of Cellomics ; Chongqing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of General Surgery and Center of Minimal Invasive; Southwest Hospital; Third Military Medical University ; Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Biomedical Analysis Center; Third Military Medical University ; Chongqing, China ; Chongqing Key Laboratory of Cellomics ; Chongqing, China.
authors.forename: Haili
authors.initials: H
authors.lastname: Yu
authors.affiliation: Biomedical Analysis Center; Third Military Medical University ; Chongqing, China ; Chongqing Key Laboratory of Cellomics ; Chongqing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guan
authors.affiliation: Department of Immunology; Duke University Medical Center ; Durham, NC USA.
authors.forename: Regina
authors.initials: R
authors.lastname: Lin
authors.affiliation: Department of Biomedical Engineering; Cockrell School of Engineering; University of Texas at Austin ; Austin, TX USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of General Surgery and Center of Minimal Invasive; Southwest Hospital; Third Military Medical University ; Chongqing, China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Yu
authors.affiliation: Chongqing Key Laboratory of Cellomics ; Chongqing, China ; Department of Immunology; Duke University Medical Center ; Durham, NC USA.
authors.forename: Qi-Jing
authors.initials: QJ
authors.lastname: Li
authors.affiliation: Biomedical Analysis Center; Third Military Medical University ; Chongqing, China ; Chongqing Key Laboratory of Cellomics ; Chongqing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
keywords: T-cell repertoire; TCR, T-cell receptor; gastric cancer; mucosal-residence; prognosis; repertoire sequencing