title: Generation of late-born neurons in the ventral spinal cord requires the coordination of retinoic acid and Notch signaling.
abstract: Neural progenitor cells generate various types of neurons and glia in a tightly regulated manner. During primary neurogenesis, retinoic acid (RA) acts earlier than Notch signaling and regulates differentiation and proliferation by upregulating proneural and neurogenic genes in the neural plate. However, the relationship between Notch signaling and the retinoid pathway during late neurogenesis remains unclear. We investigated the role of Mindbomb (Mib)-mediated Notch signaling in the differentiation of neural progenitors during late neurogenesis by overexpressing Mib and administering RA to Tg[hsp70-Mib:EGFP]. The majority of cells in the p3 domain differentiated into GABAergic Kolmer-Agduhr (KA) cells in Tg[hsp70-mib:EGFP] embryos heat-shocked during late neurogenesis, whereas these phenotypes were suppressed by exogenous RA. Our observations suggest that Mib-mediated Notch signaling plays a critical role in the temporal differentiation of neural progenitors, and that the generation of late-born KA″ cells is regulated by the interplay between Mib and RA.
authors.affiliation: Department of Chemical and Biological Engineering, Hanbat National University, Daejeon 305-719, South Korea; Genomic Design Bioengineering Company, Daejeon 306-230, South Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Ryu
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, South Korea.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, South Korea.
authors.forename: Jung Youn
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Lim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, South Korea.
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Science, Jeju National University, Jeju 690-756, South Korea.
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Hanbat National University, Daejeon 305-719, South Korea. Electronic address: yeosy@hanbat.ac.kr.
authors.forename: Sang-Yeob
authors.initials: SY
authors.lastname: Yeo
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian; D059330:GABAergic Neurons / Q000166:cytology / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051880:Receptors, Notch / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000502:physiology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D014212:Tretinoin; D044767:Ubiquitin-Protein Ligases; C471932:mib1 protein, zebrafish
keywords: Citral; Kolmer–Agduhr neuron; Mindbomb; Retinoic acid; Transgenic zebrafish