title: Serotonin, but not dopamine, controls the stress response and anxiety-like behavior in the crayfish Procambarus clarkii.
abstract: In the animal kingdom, biogenic amines are widespread modulators of the nervous system that frequently interact to control mood. Our previous investigations in crayfish (Procambarus clarkii) have established that stress induces changes in brain serotonin (5-HT) concentrations that are responsible for the appearance of anxiety-like behavior (ALB). Here, we further analyze the roles of 5-HT and another biogenic amine, dopamine (DA), on the crayfish response to stress. We show that the intensity of crayfish ALB depends on the intensity of stressful stimulation and is associated with increased concentrations of 5-HT in the brain. These 5-HT levels were significantly correlated, before, as well as after stress, with those of DA, which were approximately 3- to 5-times less abundant. However, whereas the degree of ALB was clearly correlated with brain 5-HT concentrations, it was not significantly correlated with DA. Moreover, in contrast to injections of 5-HT, DA injections were not able to elicit a stress response or ALB. In addition, 5-HT and DA levels were not modified by treatment with the anxiolytic chlordiazepoxide, confirming that suppression of ALB by this GABA-A receptor ligand acts downstream and is independent of changes in crayfish bioamine levels. Our study also provides evidence that the anxiogenic effect of 5-HT injections can be prevented by a preliminary injection of 5-HT antagonists. Altogether, our results emphasize that the rises in brain concentrations of 5-HT, but not DA, play a role in controlling the induction and the intensity of crayfish ALB.
authors.affiliation: Université de Bordeaux, 33400 Talence, France Centre National de la Recherche Scientifique (CNRS), UMR 5287, Institut des Neurosciences Cognitives et Intégratives d'Aquitaine, Avenue des Facultés, 33405 Talence Cedex, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Fossat
authors.affiliation: Université de Bordeaux, 33400 Talence, France Centre National de la Recherche Scientifique (CNRS), UMR 5287, Institut des Neurosciences Cognitives et Intégratives d'Aquitaine, Avenue des Facultés, 33405 Talence Cedex, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Bacqué-Cazenave
authors.affiliation: Université de Bordeaux, 33400 Talence, France CNRS UMR 5293, Institut des Maladies Neurodégénératives, Rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: De Deurwaerdère
authors.affiliation: Université de Bordeaux, 33400 Talence, France Centre National de la Recherche Scientifique (CNRS), UMR 5287, Institut des Neurosciences Cognitives et Intégratives d'Aquitaine, Avenue des Facultés, 33405 Talence Cedex, France daniel.cattaert@u-bordeaux.fr.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cattaert
authors.affiliation: Université de Bordeaux, 33400 Talence, France Centre National de la Recherche Scientifique (CNRS), UMR 5287, Institut des Neurosciences Cognitives et Intégratives d'Aquitaine, Avenue des Facultés, 33405 Talence Cedex, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Delbecque
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000187:drug effects* / Q000502:physiology*; D001522:Behavior, Animal / Q000187:drug effects; D001923:Brain Chemistry; D002707:Chlordiazepoxide / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology*; D004558:Electric Stimulation; D008297:Male; D011963:Receptors, GABA-A; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D011963:Receptors, GABA-A; D012702:Serotonin Antagonists; D012701:Serotonin; D002707:Chlordiazepoxide; D004298:Dopamine
keywords: 5-HT; Anxiety; Benzodiazepines; Crustacea; GABA; Octapamine