title: Novel phenol biosensor based on laccase immobilized on reduced graphene oxide supported palladium-copper alloyed nanocages.
abstract: Developing new nanomaterials is of key importance to improve the analytical performances of electrochemical biosensors. In this work, palladium-copper alloyed nanocages supported on reduced graphene oxide (RGO-PdCu NCs) were facilely prepared by a simple one-pot solvothermal method. A novel phenol biosensor based on laccase has been constructed for rapid detection of catachol, using RGO-PdCu NCs as electrode material. The as-developed phenol biosensor greatly enhanced the electrochemical signals for catechol. Under the optimal conditions, the biosensor has two linear ranges from 0.005 to 1.155 mM and 1.655 to 5.155 mM for catachol detection at 0.6 V, the sensitivity of 12.65 µA mM(-1) and 5.51 µA mM(-1), respectively. This biosensor showed high selectivity, low detection limit, good reproducibility, and high anti-interference ability.
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Mei
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Jiu-Ju
authors.initials: JJ
authors.lastname: Feng
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Chen
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: ajwang@zjnu.cn.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D002396:Catechols / Q000032:analysis* / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002396:Catechols; D004800:Enzymes, Immobilized; D010087:Oxides; D010165:Palladium; D006108:Graphite; D003300:Copper; D042845:Laccase; C034221:catechol
keywords: Alloyed nanocages; Biosensor; Catechol; Laccase; Reduced graphene oxide