title: Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum.
abstract: Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, Chaetoceros neogracile (Cn-AFP), to prevent or delay ice formation. To coat Al with Cn-AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with Cn-AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. Cn-AFP-coated Al (Cn-AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface-coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.
authors.affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.
authors.forename: Yunho
authors.initials: Y
authors.lastname: Gwak
authors.affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.
authors.forename: Ji-In
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.
authors.forename: Minjae
authors.initials: M
authors.lastname: Kim
authors.affiliation: DMC R&D Center, Samsung Electronics co. Ltd., (Meatan dong) 129, Samsung-ro Yeongtong-gu, Suwon-si, Gyeonggi-do 443-742, South Korea.
authors.forename: Hong Suk
authors.initials: HS
authors.lastname: Kim
authors.affiliation: DMC R&D Center, Samsung Electronics co. Ltd., (Meatan dong) 129, Samsung-ro Yeongtong-gu, Suwon-si, Gyeonggi-do 443-742, South Korea.
authors.forename: Myong Jong
authors.initials: MJ
authors.lastname: Kwon
authors.affiliation: DMC R&D Center, Samsung Electronics co. Ltd., (Meatan dong) 129, Samsung-ro Yeongtong-gu, Suwon-si, Gyeonggi-do 443-742, South Korea.
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Oh
authors.affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.|Institute of Nano Science and Technology, Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, 133-791, South Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Kim
authors.affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.
authors.forename: EonSeon
authors.initials: E
authors.lastname: Jin
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D021301:Antifreeze Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014199:Trehalose / Q000737:chemistry
chemical_list: D021301:Antifreeze Proteins; D014199:Trehalose; D000535:Aluminum