title: Downregulation of FOXP2 promoter human hepatocellular carcinoma cell invasion.
abstract: Hepatocellular carcinoma (HCC) is a major health concern with a high morbidity and mortality rate worldwide. However, the mechanism underlying hepatocarcinogenesis remains unclear. Forkhead box P2 (FOXP2) has been implicated in various human cancer types. However, the role of FOXP2 in HCC remains unknown. Western blot and immunohistochemistry were used to measure the expression of FOXP2 protein in HCC and adjacent normal tissues in 50 patients. Wound healing and transwell assays were used to determine the cell invasion ability. We showed that the level of FOXP2 was significantly reduced in HCC compared with the adjacent non-tumorous tissue. There was statistical significance between the expression of FOXP2 and vein invasion (P = 0.017), number of tumor nodes (P = 0.028), and AFP (P = 0.033). Low expression of FOXP2 correlated with poor survival. Moreover, wound healing and transwell assays showed that FOXP2 could decrease cell invasion and affect the expression of vimentin and E-cadherin. Our results suggested that FOXP2 expression was downregulated in HCC tumor tissues, and reduced FOXP2 expression was associated with poor overall survival. In addition, downregulation of FOXP2 significantly enhanced cell invasiveness. These findings uncover that FOXP2 might be a new prognostic factor and be closely correlated with HCC cell invasion.
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yan
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Xu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Shusen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Runzhou
authors.initials: R
authors.lastname: Ni
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China. ntdxzty@126.com.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226019, Jiangsu, People's Republic of China. ntdxzty@126.com.|Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. ntdxzty@126.com.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015820:Cadherins / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D005260:Female; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D014746:Vimentin / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C465448:FOXP2 protein, human; D051858:Forkhead Transcription Factors; D014746:Vimentin
keywords: Cell invasion; FOXP2; Human hepatocellular carcinoma (HCC); Prognosis