title: Systematic characterization and simultaneous quantification of the multiple components of Rhododendron dauricum based on high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry.
abstract: Rhododendron dauricum L. has been used as a traditional Chinese medicine to treat cough and asthma and relieve phlegm and bronchitis. In this study, a reliable method based on high-performance liquid chromatography with diode array detection and quadrupole time-of-flight tandem mass spectrometry was established to systematically identify and quantify the components in this herb for the first time. A total of 33 compounds were identified, including 24 flavonoids, six phenolic acids, two coumarins and one terpene. Among them, poriolin (17), farrerol-7-O-β-d-glucopyranoside (20), and syzalterin (30) were isolated from this plant for the first time, and quercetin-3-β-d-(6-p-hydroxy benzoyl) galactoside (19), quercetin-3-β-d-(6-p-coumaroyl) galactoside (21), and myrciacetin (23) were identified from this genus for the first time. Fragmentation pathways of flavonoids also have been investigated by electrospray ionization mass spectrometry. Moreover, seven bioactive constituents, namely, gallic acid (1), scopoletin (6), dihydroquercetin (7), quercetin (22), kaempferol (25), 8-desmethyl farrerol (27), and farrerol (28), were simultaneously quantified. The developed method has been validated and applied to analyze ten samples of R. dauricum from Hebei Province successfully. The contents of the seven compounds have been detected and compared.
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Lou
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zheng
authors.affiliation: International Department of Jinling High School, Hexi Campus, Nanjing, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.affiliation: Jiangxi Qingfeng Pharmaceutical Corporation, Ganzhou, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
keywords: Fragmentation patterns; Rhododendron dauricum; Simultaneous quantification; Systematic characterization