title: Prophylactic Ozone Administration Reduces Intestinal Mucosa Injury Induced by Intestinal Ischemia-Reperfusion in the Rat.
abstract: OBJECTIVE
  Intestinal ischemia-reperfusion injury is associated with mucosal damage and has a high rate of mortality. Various beneficial effects of ozone have been shown. The aim of the present study was to show the effects of ozone in ischemia reperfusion model in intestine.
  
  
  METHODS
  Twenty eight Wistar rats were randomized into four groups with seven rats in each group. Control group was administered serum physiologic (SF) intraperitoneally (ip) for five days. Ozone group was administered 1 mg/kg ozone ip for five days. Ischemia Reperfusion (IR) group underwent superior mesenteric artery occlusion for one hour and then reperfusion for two hours. Ozone + IR group was administered 1 mg/kg ozone ip for five days and at sixth day IR model was applied. Rats were anesthetized with ketamine∖xyzlazine and their intracardiac blood was drawn completely and they were sacrificed. Intestinal tissue samples were examined under light microscope. Levels of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), malondyaldehide (MDA), and protein carbonyl (PCO) were analyzed in tissue samples. Total oxidant status (TOS), and total antioxidant capacity (TAC) were analyzed in blood samples. Data were evaluated statistically by Kruskal Wallis test.
  
  
  RESULTS
  In the ozone administered group, degree of intestinal injury was not different from the control group. IR caused an increase in intestinal injury score. The intestinal epithelium maintained its integrity and decrease in intestinal injury score was detected in Ozone + IR group. SOD, GSH-Px, and CAT values were high in ozone group and low in IR. TOS parameter was highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group.
  
  
  CONCLUSIONS
  In the present study, IR model caused an increase in intestinal injury.In the present study, ozone administration had an effect improving IR associated tissue injury. In the present study, ozone therapy prevented intestine from ischemia reperfusion injury. It is thought that the therapeutic effect of ozone is associated with increase in antioxidant enzymes and protection of cells from oxidation and inflammation.
authors.affiliation: Department of Anesthesiology and Reanimation, Selçuk University Medical Faculty, 42100 Konya, Turkey.
authors.forename: Ozkan
authors.initials: O
authors.lastname: Onal
authors.affiliation: Department of General Surgery, Atatürk Education and Research Hospital, 06600 Ankara, Turkey.
authors.forename: Fahri
authors.initials: F
authors.lastname: Yetisir
authors.affiliation: Department of Anesthesiology and Reanimation, Atatürk Education and Research Hospital, 06600 Ankara, Turkey.
authors.forename: A Ebru Salman
authors.initials: AE
authors.lastname: Sarer
authors.affiliation: Department of Histology and Embryology, Hacettepe University Faculty of Medicine, 06012 Ankara, Turkey.
authors.forename: N Dilara
authors.initials: ND
authors.lastname: Zeybek
authors.affiliation: Ankara 4th Tuberculosis Dispensary, 06021 Ankara, Turkey.
authors.forename: C Oztug
authors.initials: CO
authors.lastname: Onal
authors.affiliation: Department of Endocrinology, Bozyaka Education and Research Hospital, 35022 İzmir, Turkey.
authors.forename: Banu
authors.initials: B
authors.lastname: Yurekli
authors.affiliation: Department of Biochemistry, Turgut Özal University Medical Faculty, 06010 Ankara, Turkey.
authors.forename: H Tugrul
authors.initials: HT
authors.lastname: Celik
authors.affiliation: Department of Histology and Embryology, Hacettepe University Faculty of Medicine, 06012 Ankara, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Sirma
authors.affiliation: Department of General Surgery, Atatürk Education and Research Hospital, 06600 Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kılıc
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress; D010126:Ozone / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D010126:Ozone