title: Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler optical coherence tomography.
abstract: Computing microvascular cerebral blood flow ([Formula: see text]) in real cortical angiograms is challenging. Here, we investigated whether the use of Doppler optical coherence tomography (DOCT) flow measurements in individual vessel segments can help in reconstructing [Formula: see text] across the entire vasculature of a truncated cortical angiogram. A [Formula: see text] computational framework integrating DOCT measurements is presented. Simulations performed on a synthetic angiogram showed that the addition of DOCT measurements, especially close to large inflowing or outflowing vessels, reduces the impact of pressure boundary conditions and estimated vessel resistances resulting in a more accurate reconstruction of [Formula: see text]. Our technique was then applied to reconstruct microvascular flow distributions in the mouse cortex down to [Formula: see text] by combining two-photon laser scanning microscopy angiography with DOCT.
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States ; Harvard-MIT Division of Health Sciences and Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States ; Ecole Polytechnique de Montreal , Department of Electrical Engineering, 2900 Boulevard Edouard-Montpetit, Montreal, Quebec H3T 1J4, Canada.
authors.forename: Louis
authors.initials: L
authors.lastname: Gagnon
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States.
authors.forename: Sava
authors.initials: S
authors.lastname: Sakadžić
authors.affiliation: Ecole Polytechnique de Montreal , Department of Electrical Engineering, 2900 Boulevard Edouard-Montpetit, Montreal, Quebec H3T 1J4, Canada.
authors.forename: Fréderic
authors.initials: F
authors.lastname: Lesage
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States.
authors.forename: Emiri T
authors.initials: ET
authors.lastname: Mandeville
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Yaseen
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States ; Harvard-MIT Division of Health Sciences and Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: David A
authors.initials: DA
authors.lastname: Boas
keywords: cerebral blood flow; microvasculature; multimodal imaging; optical coherence tomography; two-photon microscopy