title: Upregulation of miR-372 and -373 associates with lymph node metastasis and poor prognosis of oral carcinomas.
abstract: OBJECTIVE
  Oral squamous cell carcinoma (OSCC) is prevalent worldwide, and survival in OSCC has not improved significantly in the last few decades. MicroRNAs (miRNAs) have an important regulatory role in human cancer, including oral carcinogenesis. MiR-372 and miR-373 perform oncogenic and tumor-suppressive functions of between different human malignancies. This study investigated the miR-372 and miR-373 expression and their clinical implication in OSCC.
  
  
  METHODS
  Fifty patients with primary OSCC were included in the study. Primary cancer cells and matched normal oral epithelium were purified by laser capture microdissection. RNA were extracted from these samples. The expression levels of miR-372 and miR-373 in the tissue of OSCC patients were measured by quantitative reverse transcription polymerase chain reaction. The large tumor suppressor kinase 2 (LATS2) protein expression level was measured by Western blotting.
  
  
  RESULTS
  Both miR-372 and miR-373 was up-regulated in OSCC tissue relative to control mucosa. Among different clinical variables, over-expression of miR-372 and miR-373 were associated with nodal metastasis, lymphovascular invasion, and poor survival. Multivariate analysis showed that both high miR-372 and miR-373 expression were independent predictors for poor survival in OSCC. MiR-372 regulated LATS2 expression in OSCC cell lines. LATS2 expression levels are inversely correlated miR-372 in OSCC tissues.
  
  
  CONCLUSIONS
  Over-expression of miR-372 and miR-373 indicate worse survival in OSCC.
  
  
  METHODS
  N/A.
authors.affiliation: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, MacKay Memorial Hospital, Taipei, Taiwan.|Department of Dentistry, National Yang-Ming University Hospital, Yi-Lan, Taiwan.
authors.forename: Hsi-Feng
authors.initials: HF
authors.lastname: Tu
authors.affiliation: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, MacKay Memorial Hospital, Taipei, Taiwan.|Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan.
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Chang
authors.affiliation: Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Cheng
authors.affiliation: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, MacKay Memorial Hospital, Taipei, Taiwan.|Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.|Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan.
authors.forename: Chung-Ji
authors.initials: CJ
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000401:mortality*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000503:physiopathology*; D042601:Lymphatic Vessels / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000737:chemistry / Q000401:mortality*; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D048348:Reverse Transcription; D025521:Tumor Suppressor Proteins / Q000032:analysis; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C561021:MIRN372 microRNA, human; C527114:MIRN373 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: LATS2; miR-372; miR-373; oral carcinoma; prognosis