title: Two new ent-atisanes from the root of Euphorbia fischeriana Steud.
abstract: Two new ent-atisanes ent-1β,3β,16β,17-tetrahydroxyatisane (1), ent-1β,3α,16β,17-tetrahydroxyatisane (2) together with 11 known diterpenes were isolated from the anti-tumour activity fraction of Euphorbia fischeriana Steud. The compounds were identified by detailed spectroscopic analysis, including extensive 2D-NMR experiments. X-ray analysis was applied to determine the structure of compound 2. All 13 compounds were screened for cytotoxicity in vitro against human tumour MCF-7, HepG-2 and SGC-7901 cell lines. Compounds 1 and 2 showed the inhibitory effects against MCF-7 with IC50 levels of 23.21 and 15.42 μM; simultaneously, compounds 4, 6, 8 and 11 also had definite inhibitory effect against different cell lines.
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wei
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Bingyou
authors.initials: B
authors.lastname: Yang
authors.affiliation: a Key Laboratory of Chinese Materia Medica (Ministry of Education) , Heilongjiang University of Chinese Medicine , No. 24, Heping Road, Xiangfang District, Harbin 150040 , P.R. China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C000609494:ent-1beta,3beta,16beta,17-tetrahydroxyatisane
keywords: Euphorbia fischeriana Steud; X-ray; anti-tumour activity; ent-atisane; stereoismers