title: Guidance for improving comparability and relevance of oil toxicity tests.
abstract: The complex nature and limited aqueous solubility of petroleum substances pose challenges for consistently characterizing exposures in aquatic life hazard assessments. This paper reviews important considerations for the design, conduct and interpretation of laboratory toxicity tests with physically and chemically dispersed oils based on an understanding of the behavior and toxicity of the hydrocarbons that comprise these substances. Guiding principles are provided that emphasize the critical need to understand and, when possible, characterize dissolved hydrocarbon exposures that dictate observed toxicity in these tests. These principles provide a consistent framework for interpreting toxicity studies performed using different substances and test methods by allowing varying dissolved exposures to be expressed in terms of a common metric based on toxic units (TUs). The use of passive sampling methods is also advocated since such analyses provide an analytical surrogate for TUs. The proposed guidance is translated into a series of questions that can be used in evaluating existing data and in guiding design of future studies. Application of these questions to a number of recent publications indicates such considerations are often ignored, thus perpetuating the difficulty of interpreting and comparing results between studies and limiting data use in objective hazard assessment. Greater attention to these principles will increase the comparability and utility of oil toxicity data in decision-making.
authors.affiliation: ExxonMobil Biomedical Sciences, Inc., 1545 US Highway 22 East, Annandale, NJ 08801, USA. Electronic address: aaron.d.redman@exxonmobil.com.
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Redman
authors.affiliation: ExxonMobil Biomedical Sciences, Inc., 22777 Springwood Village Parkway, Spring, TX 77339, USA.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Parkerton
mesh_terms: D054750:Ecotoxicology / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D006838:Hydrocarbons / Q000633:toxicity; D010578:Petroleum / Q000633:toxicity*; D012995:Solubility; D018675:Toxicity Tests / Q000379:methods*; D014867:Water; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D014874:Water Pollutants, Chemical; D014867:Water
keywords: Crude oil; Hazard assessment; Petroleum substances; QSAR; Toxicity; Water Accomodated Fraction