title: A common variant near BDNF is associated with dietary calcium intake in adolescents.
abstract: Specific targets for most obesity candidate genes discovered by genomewide association studies remain unknown. Such genes are often highly expressed in the hypothalamus, indicating their role in energy homeostasis. We aimed to evaluate the associations of selected gene variants with adiposity and dietary traits. Anthropometric parameters, fat mass, dietary intake (total energy, fat, protein, carbohydrate, fiber, and calcium) and 10 gene variants (in/near TMEM18, SH2B1, KCTD15, PCSK1, BDNF, SEC16B, MC4R and FTO) were analyzed in 1953 Czech individuals aged 10.0 to 18.0 years (1035 nonoverweight and 918 overweight: body mass index [BMI] ≥90th percentile). Obesity risk alleles of TMEM18 rs7561317, SEC16B rs10913469, and FTO rs9939609 were related to increased body weight and BMI (P < .005). The FTO variant also showed a significant positive association with waist circumference and fat mass (P < .001). Overweight adolescents had a lower total energy intake (P < .001) but a higher percentage of fat (P = .009) and protein intake (P < .001) than the nonoverweight subjects. There was also a lower calcium intake in the overweight group (P < .001). An association with at least one component of dietary intake was found in 3 of 10 studied gene variants. The MC4R rs17782313 was associated negatively with protein (P = .012) and positively associated with fiber (P = .032) intakes. The obesity risk alleles of BDNF rs925946 and FTO rs9939609 were related to a lower calcium intake (P = .001 and .037). The effects of FTO and MC4R variants, however, disappeared after corrections for multiple testing. Our results suggest that the common BDNF variant may influence dietary calcium intake independent of BMI.
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic; Faculty of Science, Charles University in Prague, Albertov 6, CZ-128 43 Prague, Czech Republic. Electronic address: ldusatkova@gmail.com.
authors.forename: Lenka
authors.initials: L
authors.lastname: Dušátková
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Zamrazilová
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic; Department of Pediatrics and Center for Research of Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University in Prague, Šrobárova 50, CZ-100 34 Prague, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Aldhoon-Hainerová
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic; Faculty of Science, Charles University in Prague, Albertov 6, CZ-128 43 Prague, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Sedláčková
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Včelák
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Hlavatý
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic.
authors.forename: Běla
authors.initials: B
authors.lastname: Bendlová
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Kunešová
authors.affiliation: Institute of Endocrinology, Národní 8, CZ-116 94 Prague, Czech Republic.
authors.forename: Vojtěch
authors.initials: V
authors.lastname: Hainer
mesh_terms: D000293:Adolescent; D000483:Alleles; D015992:Body Mass Index; D001835:Body Weight; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D002136:Calcium, Dietary / Q000008:administration & dosage*; D002648:Child; D018153:Czech Republic; D002149:Energy Intake; D005247:Feeding Behavior; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D050177:Overweight; D020641:Polymorphism, Single Nucleotide; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002136:Calcium, Dietary; C470072:MC4R protein, human; D044105:Receptor, Melanocortin, Type 4; C579455:BDNF protein, human
keywords: Adolescence; BDNF; Dietary intake; FTO; Obesity; Single nucleotide polymorphism