title: Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus).
abstract: Infection with Helicobacter pylori strains containing high number of EPIYA-C phosphorylation sites in the CagA is associated with significant gastritis and increased risk of developing pre-malignant gastric lesions and gastric carcinoma. However, these findings have not been reproduced in animal models yet. Therefore, we investigated the effect on the gastric mucosa of Mongolian gerbil (Meriones unguiculatus) infected with CagA-positive H. pylori strains exhibiting one or three EPIYA-C phosphorilation sites. Mongolian gerbils were inoculated with H. pylori clonal isolates containing one or three EPIYA-C phosphorylation sites. Control group was composed by uninfected animals challenged with Brucella broth alone. Gastric fragments were evaluated by the modified Sydney System and digital morphometry. Clonal relatedness between the isolates was considered by the identical RAPD-PCR profiles and sequencing of five housekeeping genes, vacA i/d region and of oipA. The other virulence markers were present in both isolates (vacA s1i1d1m1, iceA2, and intact dupA). CagA of both isolates was translocated and phosphorylated in AGS cells. After 45 days of infection, there was a significant increase in the number of inflammatory cells and in the area of the lamina propria in the infected animals, notably in those infected by the CagA-positive strain with three EPIYA-C phosphorylation sites. After six months of infection, a high number of EPIYA-C phosphorylation sites was associated with progressive increase in the intensity of gastritis and in the area of the lamina propria. Atrophy, intestinal metaplasia, and dysplasia were also observed more frequently in animals infected with the CagA-positive isolate with three EPIYA-C sites.  We conclude that infection with H. pylori strain carrying a high number of CagA EPIYA-C phosphorylation sites is associated with more severe gastric lesions in an animal model of H. pylori infection.
authors.affiliation: Federal University of Minas Gerais. moacirferreirasantana@yahoo.com.br.
authors.forename: M
authors.initials: M
authors.lastname: Ferreira Júnior
authors.forename: S A
authors.initials: SA
authors.lastname: Batista
authors.forename: P V T
authors.initials: PV
authors.lastname: Vidigal
authors.forename: A A C
authors.initials: AA
authors.lastname: Cordeiro
authors.forename: F M S
authors.initials: FM
authors.lastname: Oliveira
authors.forename: L O
authors.initials: LO
authors.lastname: Prata
authors.forename: A E T
authors.initials: AE
authors.lastname: Diniz
authors.forename: C M
authors.initials: CM
authors.lastname: Barral
authors.forename: R C
authors.initials: RC
authors.lastname: Barbuto
authors.forename: A D
authors.initials: AD
authors.lastname: Gomes
authors.forename: I D
authors.initials: ID
authors.lastname: Araújo
authors.forename: D M M
authors.initials: DM
authors.lastname: Queiroz
authors.forename: M V
authors.initials: MV
authors.lastname: Caliari
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D018450:Disease Progression; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D021381:Protein Transport; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology; D014510:Urease / Q000032:analysis / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori; D014510:Urease