title: Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection.
abstract: A label-free fluorescence assay for rapid and sensitive detection of heparin (Hep) or chondroitin sulfate (CS) was developed by guanidine-terminated poly (amidoanime) (PAMAM-Gu(+)) dendrimers induced aggregation of graphene quantum dots (GQDs). The fluorescence of GQDs was obviously quenched after mixing with PAMAM-Gu(+). However, the addition of highly negatively charged Hep or CS into the fluorescence sensing system resulted in the fluorescence recovery. Because the multi-positively charged PAMAM-Gu(+) would prefer to bind with highly negatively charged Hep or CS, resulting in the deaggregation of GQDs. Under the optimized experimental conditions, the recovery of fluorescence intensity ratio I/I0 (I0 and I were the fluorescence intensity of the sensing system in the absence or presence of target analytes, respectively) was proportional to the concentration of target analytes in the range of 0.04-1.6 μg mL(-1) for Hep and 0.1-2.5 μg mL(-1) for CS. In addition, this method afforded high sensitivity with the detection limit as low as 0.02 μg mL(-1) and 0.05 μg mL(-1) for Hep and CS, respectively. All results suggested that the fluorescence turn-on method could be successfully employed for sensitive and selective detection of heparin analogs.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Fanping
authors.initials: F
authors.lastname: Shi
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Lehui
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000925:Anticoagulants / Q000032:analysis*; D002809:Chondroitin Sulfates / Q000032:analysis*; D050091:Dendrimers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D019791:Guanidine / Q000031:analogs & derivatives; D006493:Heparin / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000925:Anticoagulants; D050091:Dendrimers; D005456:Fluorescent Dyes; C104700:PAMAM Starburst; D006108:Graphite; D006493:Heparin; D002809:Chondroitin Sulfates; D019791:Guanidine
keywords: Chondroitin sulfate.; Fluorescence; Graphene quantum dots; Guanidyl dendrimer; Heparin