title: Automated identification of keratinization and keratin pearl area from in situ oral histological images.
abstract: Oral squamous cell carcinoma (OSCC) has contributed 90% of oral cancer worldwide. In situ histological evaluation of tissue sections is the gold standard for oral cancer detection. Formation of keratinization and keratin pearl is one of the most important histological features for OSCC grading. This paper aims at developing a computer assisted quantitative microscopic methodology for automated identification of keratinization and keratin pearl area from in situ oral histological images. The proposed methodology includes colour space transform in YDbDr channel, enhancement of keratinized area in most significant bit (MSB) plane of Db component, segmentation of keratinized area using Chan-Vese model. The proposed methodology achieves 95.08% segmentation accuracy in comparison with (manually) experts-based ground truths. In addition, a grading index describing keratinization area is explored for grading OSCC cases (poorly, moderately and well differentiated).
authors.affiliation: School of Medical Science and Technology, IIT Kharagpur, India.
authors.forename: Dev Kumar
authors.initials: DK
authors.lastname: Das
authors.affiliation: School of Medical Science and Technology, IIT Kharagpur, India. Electronic address: chandanc@smst.iitkgp.ernet.in.
authors.forename: Chandan
authors.initials: C
authors.lastname: Chakraborty
authors.affiliation: Tata Medical Center, Kolkata, India.
authors.forename: Satyakam
authors.initials: S
authors.lastname: Sawaimoon
authors.affiliation: Midnapur Medical College and Hospital, West Bengal, India.
authors.forename: Asok Kumar
authors.initials: AK
authors.lastname: Maiti
authors.affiliation: Tata Medical Center, Kolkata, India.
authors.forename: Sanjoy
authors.initials: S
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000473:pathology; D003952:Diagnostic Imaging; D006801:Humans; D007633:Keratins / Q000302:isolation & purification*; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000473:pathology; D060787:Neoplasm Grading
chemical_list: D007633:Keratins
keywords: Bit-plane slicing; Chan–Vese model; Grading index; Keratin pearl; Keratinization; Oral cancer; Squamous cell carcinoma