title: Enterobacter Strains Might Promote Colon Cancer.
abstract: Many studies have been performed to determine the interaction between bacterial species and cancer. However, there has been no attempts to demonstrate a possible relationship between Enterobacter spp. and colon cancer so far. Therefore, in the present study, it is aimed to investigate the effects of Enterobacter strains on colon cancer. Bacterial proteins were isolated from 11 Enterobacter spp., one Morganella morganii, and one Escherichia coli strains, and applied onto NCM460 (Incell) and CRL1790 (ATCC) cell lines. Cell viability and proliferation were determined in MTS assay. Flow Cytometry was used to detect CD24 level and apoptosis. Real-Time PCR studies were performed to determine NFKB and Bcl2 expression. Graphpad Software was used for statistical analysis. The results showed that proteins, isolated from the Enterobacter spp., have significantly increased cell viability and proliferation, while decreasing the apoptosis of the cell lines tested. The data in the present study indicated that Enterobacter strains might promote colon cancer. Moreover, Enterobacter spp. could be a clinically important factor for colon cancer initiation and progression. Studies can be extended on animal models in order to develop new strategies for treatment.
authors.affiliation: Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
authors.forename: Dilşad
authors.initials: D
authors.lastname: Yurdakul
authors.forename: Ayten
authors.initials: A
authors.lastname: Yazgan-Karataş
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Şahin
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D051927:CD24 Antigen / Q000032:analysis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000209:etiology; D004754:Enterobacter / Q000737:chemistry*; D004756:Enterobacteriaceae Infections / Q000150:complications; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology*; D004926:Escherichia coli / Q000737:chemistry; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D020613:Morganella morganii / Q000737:chemistry; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D051927:CD24 Antigen; C497889:CD24 protein, human; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2