title: ADIPOQ single nucleotide polymorphism: Association with adiponectin and lipoproteins levels restricted to men.
abstract: Adiponectin is an adipokine inversely correlated with obesity, which has beneficial effect on insulin resistance and lipid metabolism. Considering its potential as a therapeutic target in the metabolic disorder contexts, and in order to add knowledge in the area, our study evaluated the ADIPOQ 276G > T polymorphism effect on adiponectin levels, and on lipoproteins of clinical interest in a population sample composed of 211 healthy individuals. Significant effects were observed only among men: the carriers of heterozygous genotype (GT) showed high levels of adiponectin (p = 0.018), while the rare homozygous genotype (TT) gave its carriers a negative phenotype, represented by higher levels of low density lipoprotein cholesterol (LDL-C) (p = 0.004 and p = 0.005) and total cholesterol (TC) (p = 0.010 and p = 0.005) compared to carriers of other genotypes (GG and GT respectively), the independent effect of SNP on LDL-C and TC levels was confirmed by multiple regression analysis (p = 0.008 and p = 0.044). We found no evidence of correlation between circulating adiponectin levels and biochemical markers, which suggests, therefore, an SNP 276G > T independent effect on adiponectin levels and on lipoprotein metabolism in men enrolled in this study.
authors.affiliation: Polymorphism and Linkage Laboratory, Department of Genetics, Federal University of Paraná, Brazil.
authors.forename: Luciane Viater
authors.initials: LV
authors.lastname: Tureck
authors.affiliation: Department of Physical Education, Federal University of Paraná, Brazil.
authors.forename: Neiva
authors.initials: N
authors.lastname: Leite
authors.affiliation: Polymorphism and Linkage Laboratory, Department of Genetics, Federal University of Paraná, Brazil.
authors.forename: Ricardo Lehtonen Rodrigues
authors.initials: RL
authors.lastname: Souza
authors.affiliation: Department of Physical Education, Federal University of Paraná, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: da Silva Timossi
authors.affiliation: Department of Physical Education, Federal University of Paraná, Brazil.
authors.forename: Ana Claudia Vecchi
authors.initials: AC
authors.lastname: Osiecki
authors.affiliation: Department of Physical Education, Federal University of Paraná, Brazil.
authors.forename: Raul
authors.initials: R
authors.lastname: Osiecki
authors.affiliation: Polymorphism and Linkage Laboratory, Department of Genetics, Federal University of Paraná, Brazil.
authors.forename: Lupe Furtado
authors.initials: LF
authors.lastname: Alle
keywords: 276G > T SNP; ADIPOQ gene; AMPK, adenosine monophosphate-activated protein kinase; Adiponectin; BMI, body mass index; ELISA, enzyme-Linked Immunosorbent assay; GWA study, genome-wide association study; Gender effect; HDL-C, high density lipoprotein cholesterol; HL, hepatic lipase; IDL, intermediate density lipoproteins; LDL-C, low density lipoprotein cholesterol; LDLR, LDL-C receptor; LPL, lipoprotein lipase; Lipid metabolism; SNP, single-nucleotide polymorphism; TC, total cholesterol; TG, triglycerides; VLDL, very low density lipoproteins