title: Influence of glucose fermentation on CO₂ assimilation to acetate in homoacetogen Blautia coccoides GA-1.
abstract: Fermentation of glucose influences CO2 assimilation to acetate in homoacetogens. Blautia coccoides was investigated for a better understanding of the metabolic characteristics of homoacetogens in mixotrophic cultures. Batch cultures of the strain with H2/CO2 as a sole carbon source reached an acetate yield of 5.32 g/g dry cell weight after 240 h of incubation. Autotrophic metabolism was inhibited as glucose was added into the culture: the higher the glucose concentration the lower the autotrophic ability of the bacterium. Autotrophy was inhibited by high glucose concentration, probably due to the competition for coenzyme A between the Embden-Meyerhof-Parnas pathway and the Wood-Ljungdahl carbon fixation pathway, the energy (adenosine triphosphate) allocation for synthesis of cell carbon and reduction of CO2, in combination with the low pH caused by the accumulation of acetate.
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Liu
authors.forename: Jianzheng
authors.initials: J
authors.lastname: Li
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Antwi
authors.initials: A
authors.lastname: Philip
authors.forename: En
authors.initials: E
authors.lastname: Shi
authors.forename: Xue
authors.initials: X
authors.lastname: Chi
authors.forename: Jia
authors.initials: J
authors.lastname: Meng
mesh_terms: D000085:Acetates / Q000378:metabolism*; D052818:Autotrophic Processes; D057486:Carbon Cycle; D002245:Carbon Dioxide / Q000378:metabolism*; D000068016:Eubacteriales / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D062065:Wastewater / Q000382:microbiology
chemical_list: D000085:Acetates; D002245:Carbon Dioxide; D005947:Glucose; D062065:Wastewater