title: Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss.
abstract: MTA, Bio-Oss, and dentin chips have been successfully used in endodontics. The aim of this study was to assess the adhesion and migration of dental stem cells on human pulp ceiling cavities filled with these endodontic materials in an experimental model, which mimics the clinical conditions of regenerative endodontics. Cavities were formed, by a homemade mold, on untouched third molars, filled with endodontic materials, and observed with electron microscopy. Cells were seeded on cavities' surface and their morphology and number were analysed. The phenomenon of tropism was assessed in a migration assay. All three materials demonstrated appropriate microstructures for cell attachment. Cells grew on all reagents, but they showed a differential morphology. Moreover, variations were observed when comparing cells numbers on cavity's filling versus the surrounding dentine disc. The highest number of cells was recorded on dentin chips whereas the opposite was true for Bio-Oss. This was confirmed in the migration assay where a statistically significant lower number of cells migrated towards Bio-Oss as compared to MTA and dentin chips. This study highlights that MTA and dentin chips have a greater potential compared to Bio-Oss regarding the attraction of dental stem cells and are good candidates for bioengineered pulp regeneration.
authors.affiliation: Department of Endodontics, Dental School, University of Athens, Athens, Greece ; Department of Genetics and Gene Therapy, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
authors.forename: Stefania
authors.initials: S
authors.lastname: Lymperi
authors.affiliation: Department of Genetics and Gene Therapy, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Taraslia
authors.affiliation: Department of Endodontics, Dental School, University of Athens, Athens, Greece.
authors.forename: Ioannis N
authors.initials: IN
authors.lastname: Tsatsoulis
authors.affiliation: University of Oslo, Postboks 1103, Blindern, 0317 Oslo, Norway.
authors.forename: Athina
authors.initials: A
authors.lastname: Samara
authors.affiliation: Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece.
authors.forename: Athanasios D
authors.initials: AD
authors.lastname: Velentzas
authors.affiliation: Department of Endodontics, Dental School, University of Athens, Athens, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Agrafioti
authors.affiliation: Department of Genetics and Gene Therapy, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
authors.forename: Ema
authors.initials: E
authors.lastname: Anastasiadou
authors.affiliation: Department of Endodontics, Dental School, University of Athens, Athens, Greece.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Kontakiotis
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology*; D003786:Dental Pulp Cavity / Q000378:metabolism / Q000473:pathology; D003804:Dentin / Q000378:metabolism; D006801:Humans; D008903:Minerals / Q000008:administration & dosage*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: C077540:Bio-Oss; D008903:Minerals