title: Hybrid core/shell microparticles and their use for understanding biological processes.
abstract: Hybrid micro and nanoparticles have become a topic of intense research in recent years. This is due to the special properties of these materials that open new avenues in advanced applications. Herein, we report a novel method for the generation of hybrid particles utilising plasma polymerization. Poly (methyl methacrylate) (PMMA) beads were first coated with a thin allylamine based plasma polymer layer. Gold nanoparticles of engineered size and surface structure were then attached in a controlled manner to the plasma polymer coated beads. To generate uniform chemistry on the outermost surface and to preserve the nanotopography, we deposited a 5-10 nm thin layer of Acpp. We demonstrated that these particles can be utilized in in vivo models to interrogate important biological phenomena. Specifically, we used them in mice to study the inflammatory and foreign body responses to surface nanotopography. The data strongly indicates that surface nanotopography and chemistry can modulate collagen production and the number of adhering immune cells. The method for generating hybrid particles reported here is solvent free and can open new opportunities in fields such as tissue engineering, drug delivery, biosensors, and regenerative medicine.
authors.affiliation: Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Akash
authors.initials: A
authors.lastname: Bachhuka
authors.affiliation: Experimental Therapeutics Laboratory, Royal Adelaide Hospital, Adelaide, SA 5000, Australia; Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Susan N
authors.initials: SN
authors.lastname: Christo
authors.affiliation: Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Engineering, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Cavallaro
authors.affiliation: Experimental Therapeutics Laboratory, Royal Adelaide Hospital, Adelaide, SA 5000, Australia; Robinson Research Institute, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Kerrilyn R
authors.initials: KR
authors.lastname: Diener
authors.affiliation: The Australian Wine Research Institute, Urrbrae, Adelaide, SA 5000, Australia.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Mierczynska
authors.affiliation: Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Engineering, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Louise E
authors.initials: LE
authors.lastname: Smith
authors.affiliation: School of Engineering, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Romeo
authors.initials: R
authors.lastname: Marian
authors.affiliation: Centre for Neurological Diseases, SA Pathology, Adelaide, SA 5000, Australia.
authors.forename: Jim
authors.initials: J
authors.lastname: Manavis
authors.affiliation: Experimental Therapeutics Laboratory, Royal Adelaide Hospital, Adelaide, SA 5000, Australia; Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Medicine, University of Adelaide, Adelaide, SA 5005, Australia. Electronic address: john.hayball@unisa.edu.au.
authors.forename: John D
authors.initials: JD
authors.lastname: Hayball
authors.affiliation: Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia; School of Engineering, University of South Australia, Adelaide, SA 5000, Australia. Electronic address: krasimir.vasilev@unisa.edu.au.
authors.forename: Krasimir
authors.initials: K
authors.lastname: Vasilev
mesh_terms: D000499:Allylamine / Q000737:chemistry*; D000818:Animals; D005549:Foreign-Body Reaction; D006046:Gold / Q000737:chemistry*; D007249:Inflammation / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D008832:Microchemistry; D010316:Particle Size; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000499:Allylamine; D006046:Gold; D019904:Polymethyl Methacrylate
keywords: Hybrid particles; Macrophages; Nanoparticles; Nanotopography; Neutrophils; Plasma polymerization; Surface chemistry