title: Comprehensive comparison of large-scale tissue expression datasets.
abstract: For tissues to carry out their functions, they rely on the right proteins to be present. Several high-throughput technologies have been used to map out which proteins are expressed in which tissues; however, the data have not previously been systematically compared and integrated. We present a comprehensive evaluation of tissue expression data from a variety of experimental techniques and show that these agree surprisingly well with each other and with results from literature curation and text mining. We further found that most datasets support the assumed but not demonstrated distinction between tissue-specific and ubiquitous expression. By developing comparable confidence scores for all types of evidence, we show that it is possible to improve both quality and coverage by combining the datasets. To facilitate use and visualization of our work, we have developed the TISSUES resource (http://tissues.jensenlab.org), which makes all the scored and integrated data available through a single user-friendly web interface.
authors.affiliation: Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Alberto
authors.initials: A
authors.lastname: Santos
authors.affiliation: Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Tsafou
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Sydney, Australia.
authors.forename: Christian
authors.initials: C
authors.lastname: Stolte
authors.affiliation: Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Sune
authors.initials: S
authors.lastname: Pletscher-Frankild
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Sydney, Australia.|Garvan Institute of Medical Research, Sydney, Australia.
authors.forename: Seán I
authors.initials: SI
authors.lastname: O'Donoghue
authors.affiliation: Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Lars Juhl
authors.initials: LJ
authors.lastname: Jensen
keywords: Databases; Immunohistochemistry; Mass spectrometry; Microarrays; RNA sequencing; Tissue expression; Tissue-specificity