title: Expression Levels of Myostatin and Matrix Metalloproteinase 14 mRNAs in Uterine Leiomyoma are Correlated With Dysmenorrhea.
abstract: Uterine leiomyoma is the most common benign neoplasm of female reproductive system, found in about 50% of women in reproductive age. The mechanisms of leiomyoma growth include cell proliferation, which is modulated by growth factors, and deposition of extracellular matrix (ECM). Activin A and myostatin are growth factors that play a role in proliferation of leiomyoma cells. Matrix metalloproteinases (MMPs) are known for their ability to remodel the ECM in different biological systems. The aim of this study was to evaluate the expression levels of activin βA-subunit, myostatin, and MMP14 messenger RNAs (mRNAs) in uterine leiomyomas and the possible correlation of these factors with clinical features of the disease. Matrix metalloproteinase 14 was highly expressed in uterine leiomyoma and correlated with myostatin and activin A mRNA expression. Moreover, MMP14 and myostatin mRNA expression correlated significantly and directly with the intensity of dysmenorrhea. Overall, the present findings showed that MMP14 mRNA is highly expressed in uterine leiomyoma, where it correlates with the molecular expression of growth factors and is further increased in cases of intense dysmenorrhea.
authors.affiliation: Department of Biological Sciences, Xi'an Jiaotong Liverpool University, Suzhou, China anastasia.tsigkou@xjtlu.edu.cn.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Tsigkou
authors.affiliation: Division of Human Reproduction, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Reis
authors.affiliation: Department of Experimental and Clinical Medicine, Polytechnic University of Marche, Ancona, Italy Department of Information Engineering, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Pasquapina
authors.initials: P
authors.lastname: Ciarmela
authors.affiliation: Department of Biological Sciences, Xi'an Jiaotong Liverpool University, Suzhou, China.
authors.forename: Meng H
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Guangzhou University, Panyu, Guangzhou, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Molecular and Developmental Medicine, Obstetrics and Gynecology, University of Siena, Siena, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Tosti
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Shen
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Zhendan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: You-Guo
authors.initials: YG
authors.lastname: Chen
authors.affiliation: Department of Molecular and Developmental Medicine, Obstetrics and Gynecology, University of Siena, Siena, Italy felice.petraglia@unisi.it.
authors.forename: Felice
authors.initials: F
authors.lastname: Petraglia
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D004412:Dysmenorrhea / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D028322:Inhibin-beta Subunits / Q000235:genetics; D007889:Leiomyoma / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D053511:Matrix Metalloproteinase 14 / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D055435:Myostatin / Q000032:analysis / Q000235:genetics*; D011446:Prospective Studies; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D014594:Uterine Neoplasms / Q000150:complications / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C525911:MSTN protein, human; D055435:Myostatin; D012333:RNA, Messenger; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: MMP14; abnormal uterine bleeding; activin-A; dysmenorrhea; leiomyoma; matrix metalloproteinase; myostatin