title: In vitro antifungal activity of extracts obtained from Hypericum perforatum adventitious roots cultured in a mist bioreactor against planktonic cells and biofilm of Malassezia furfur.
abstract: Xanthone-rich extracts from Hypericum perforatum root cultures grown in a Mist Bioreactor as antifungal agents against Malassezia furfur. Extracts of Hypericum perforatum roots grown in a bioreactor showed activity against planktonic cells and biofilm of Malassezia furfur. Dried biomass, obtained from roots grown under controlled conditions in a ROOTec mist bioreactor, has been extracted with solvents of increasing polarity (i.e. chloroform, ethyl acetate and methanol). The methanolic fraction was the richest in xanthones (2.86 ± 0.43 mg g(-1) DW) as revealed by HPLC. The minimal inhibitory concentration of the methanol extract against M. furfur planktonic cells was 16 μg mL(-1). The inhibition percentage of biofilm formation, at a concentration of 16 μg mL(-1), ranged from 14% to 39%. The results show that H. perforatum root extracts could be used as new antifungal agents in the treatment of Malassezia infections.
authors.affiliation: a Department of Public Health and Infectious Diseases , Sapienza Università di Roma , Rome , Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Simonetti
authors.affiliation: b Department of Environmental Biology , Sapienza Università di Roma , Rome , Italy.
authors.forename: Noemi
authors.initials: N
authors.lastname: Tocci
authors.affiliation: b Department of Environmental Biology , Sapienza Università di Roma , Rome , Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Valletta
authors.affiliation: b Department of Environmental Biology , Sapienza Università di Roma , Rome , Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Brasili
authors.affiliation: a Department of Public Health and Infectious Diseases , Sapienza Università di Roma , Rome , Italy.
authors.forename: Felicia Diodata
authors.initials: FD
authors.lastname: D'Auria
authors.affiliation: c ROOTec Bioactives AG , Benkenstrasse 254, 4108 Witterswil , Switzerland.
authors.forename: Alicia
authors.initials: A
authors.lastname: Idoux
authors.affiliation: b Department of Environmental Biology , Sapienza Università di Roma , Rome , Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pasqua
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D020902:Hypericum / Q000737:chemistry*; D008290:Malassezia / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts; D044004:Xanthones
keywords: Hypericum perforatum; Malassezia furfur; antifungal activity; biofilm; bioreactor; root cultures; xanthones