title: Isotope-Coded Labeling for Accelerated Protein Interaction Profiling Using MS.
abstract: Protein interaction surface mapping using MS is widely applied but comparatively resource-intensive. Here, a workflow adaptation for use of isotope-coded tandem mass tags for the purpose is reported. The key benefit of improved throughput derived from sample acquisition multiplexing and automated analysis is shown to be maintained in the new application. Mapping of the epitopes of two monoclonal antibodies on their respective targets serves to illustrate the novel approach. We conclude that the approach enables mapping of interactions by MS at significantly larger scales than hereto possible.
authors.affiliation: †Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.
authors.forename: John D
authors.initials: JD
authors.lastname: Venable
authors.affiliation: †Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.|‡Joint Center for Structural Genomics, La Jolla, California 92037, United States, http://www.jcsg.org.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Steckler
authors.affiliation: †Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.
authors.forename: Weijia
authors.initials: W
authors.lastname: Ou
authors.affiliation: †Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.
authors.forename: Jan
authors.initials: J
authors.lastname: Grünewald
authors.affiliation: †Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Agarwalla
authors.affiliation: †Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.
authors.forename: Ansgar
authors.initials: A
authors.lastname: Brock
mesh_terms: D001665:Binding Sites; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D008958:Models, Molecular; D025941:Protein Interaction Mapping / Q000379:methods*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins