title: Comparison between cryopreserved and glycerol-preserved allografts in a partial-thickness porcine wound model.
abstract: Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clinical conditions, we used a porcine wound model. In addition, we evaluated the macroscopic and microscopic scoring of graft performance for each method. Porcine cadaver skin 1 mm thick was obtained from one pig. Cryopreserved skin cell viability was 20.8 %, glycerol-preserved skin was 9.08 %, and fresh skin was 58.6 %. We made ten partial-thickness wounds each in two pigs. The take rates on day 2 were 96.23 and 82.65 % in the GPA and CPA group (both n = 9), respectively. After 1 week, the take rates of both groups were nearly equal. The removal rate at week 5 was 98.87 and 94.41 % in the GPA and CPA group, respectively. On microscopic findings at week 2, inflammation was greater in the CPA group. Other findings such as fibroblast hyperplasia and neovascularization were not significantly different between both groups. At week 5, the score of collagen fiber synthesis was 2.67 ± 0.47 and 2.33 ± 0.47 in the GPA and CPA group, respectively. The epidermal-dermal junction was 2.22 ± 0.79 and 2.00 ± 0.47 in the GPA and CPA group, respectively. These findings suggest that wound healing takes longer in the CPA group. The preservation method of allografts is not a absolute factor in the wound healing process in this wound model.
authors.affiliation: Department of Emergency Medicine, Bestian Seoul Hospital, Seoul, Korea.
authors.forename: Cheonjae
authors.initials: C
authors.lastname: Yoon
authors.affiliation: Medical Group Bestian, Bestian R&D Center, Daejeon, Korea.
authors.forename: Kihwan
authors.initials: K
authors.lastname: Lim
authors.affiliation: Medical Group Bestian, Bestian R&D Center, Daejeon, Korea.
authors.forename: Sungjun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Emergency Medicine, Bestian Daejeon Hospital, Daejeon, Korea.
authors.forename: Yanghwan
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Emergency Medicine, Bestian Seoul Hospital, Seoul, Korea.
authors.forename: Youngwhan
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Medical Group Bestian, Bestian R&D Center, Daejeon, Korea. nostalgie@naver.com.
authors.forename: Jungsuk
authors.initials: J
authors.lastname: Lee
mesh_terms: D064591:Allografts / Q000187:drug effects*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D004195:Disease Models, Animal; D005990:Glycerol / Q000494:pharmacology*; D007249:Inflammation / Q000473:pathology; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature; D014022:Tissue Survival / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects; D014947:Wounds and Injuries / Q000473:pathology*
chemical_list: D003094:Collagen; D005990:Glycerol
keywords: Cryopreserved skin allograft; Glycerol-preserved skin allograft; Porcine; Wound healing