title: The effect of treadmill training and N-acetyl-l-cysteine intervention on biogenesis of cytochrome c oxidase (COX).
abstract: Mitochondrial biogenesis refers to increased content of mitochondria, which has been shown to be promoted by aerobic exercise. During this process, oxidative stress is considered the essential initiator. Even though some studies have addressed the issue as to whether antioxidants would hamper the effects of exercise on mitochondrial biogenesis, no consensus has been achieved. Therefore, the purpose of the present study was to investigate the effects of exercise and antioxidant intervention on mitochondrial biogenesis, as well as COX biogenesis. Thirty-two clean-grade male ICR mice were randomly assigned to a control group (Con), exercise group (Ex), N-acetyl-l-cysteine group (NAC), or NAC plus exercise group (NEx). The NAC and NEx groups were injected with NAC (0.1 mg/g/2 days) intraperitoneally for 3 weeks, whereas the Con and Ex groups were administered saline for the same period of time. Mice assigned to Ex and NEx groups started exercise training 1 week before drug intervention was initiated. After 1 week of acclimatization, the mice were allowed to run at a speed of 28 m/min for 60 min, 6 days a week. The results showed that exercise training caused an increase in mRNA and protein levels of COXIV, whereas NAC intervention lowered the two so significantly that even exercise training could not reverse the effect of NAC intervention. Our data suggest that even though antioxidant intervention could alleviate oxidative damage caused by exercise, it was not necessarily beneficial for mitochondrial biogenesis.
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Qi
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China.
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China.
authors.forename: D
authors.initials: D
authors.lastname: Cui
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China.
authors.forename: S
authors.initials: S
authors.lastname: Qian
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China. Electronic address: lji@tyxx.ecnu.edu.cn.
authors.forename: L
authors.initials: L
authors.lastname: Ji
authors.affiliation: Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, China; School of Physical Education & Health Care, East China Normal University, Shanghai 200241, China. Electronic address: szding@tyxx.ecnu.edu.cn.
authors.forename: S
authors.initials: S
authors.lastname: Ding
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D045304:Cytochromes c; D003576:Electron Transport Complex IV; D000111:Acetylcysteine
keywords: COX biogenesis; Exercise; NAC; Oxidative stress